LOCUS       DAWFKN010000001        95191 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFKN010000001 DAWFKN010000000
VERSION     DAWFKN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 95191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.001"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            52..1005
                     /locus_tag="WGR46_000001"
     CDS             52..1005
                     /locus_tag="WGR46_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0855036.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WGR46_000002"
     CDS             complement(1116..1439)
                     /locus_tag="WGR46_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855037.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WGR46_000003"
     CDS             complement(1612..2973)
                     /locus_tag="WGR46_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0855038.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGR46_000004"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGR46_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0855039.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5752)
                     /locus_tag="WGR46_000005"
     CDS             complement(5039..5752)
                     /locus_tag="WGR46_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855040.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTFMLWGMNDPFSQTTDRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5799..6311)
                     /locus_tag="WGR46_000006"
     CDS             complement(5799..6311)
                     /locus_tag="WGR46_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0855041.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6458..7789
                     /gene="pmbA"
                     /locus_tag="WGR46_000007"
     CDS             6458..7789
                     /gene="pmbA"
                     /locus_tag="WGR46_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0855042.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8086..8289)
                     /locus_tag="WGR46_000008"
     CDS             complement(8086..8289)
                     /locus_tag="WGR46_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0855043.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8532..8834
                     /gene="clpS"
                     /locus_tag="WGR46_000009"
     CDS             8532..8834
                     /gene="clpS"
                     /locus_tag="WGR46_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0855044.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8836..11115
                     /gene="clpA"
                     /locus_tag="WGR46_000010"
     CDS             8836..11115
                     /gene="clpA"
                     /locus_tag="WGR46_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0855045.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11162..13033)
                     /locus_tag="WGR46_000011"
     CDS             complement(11162..13033)
                     /locus_tag="WGR46_000011"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0855046.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(12994..13365)
                     /locus_tag="WGR46_000012"
                     /pseudo
     CDS             complement(12994..13365)
                     /locus_tag="WGR46_000012"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13384..14259)
                     /locus_tag="WGR46_000013"
     CDS             complement(13384..14259)
                     /locus_tag="WGR46_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0855047.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14338..15558)
                     /locus_tag="WGR46_000014"
     CDS             complement(14338..15558)
                     /locus_tag="WGR46_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0855048.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGIADGYKGRLKDVVWKTLGEVF
                     DLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNSLKHISKSAVKGGKLFPAKS
                     IMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLI
                     NTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTLTHSISEGLPHEIALRRKQY
                     EYYREQLLAFPKAT"
     gene            complement(15724..16734)
                     /locus_tag="WGR46_000015"
     CDS             complement(15724..16734)
                     /locus_tag="WGR46_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0855049.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16731..18275)
                     /locus_tag="WGR46_000016"
     CDS             complement(16731..18275)
                     /locus_tag="WGR46_000016"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0855050.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WGR46_000017"
     CDS             complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WGR46_000017"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0855051.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19393..20511)
                     /locus_tag="WGR46_000018"
     CDS             complement(19393..20511)
                     /locus_tag="WGR46_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0855052.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WGR46_000019"
     CDS             complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WGR46_000019"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0855053.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WGR46_000020"
     CDS             complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WGR46_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0855054.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WGR46_000021"
     CDS             complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WGR46_000021"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0855055.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WGR46_000022"
     CDS             complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WGR46_000022"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0855056.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24939..25778
                     /gene="htpX"
                     /locus_tag="WGR46_000023"
     CDS             24939..25778
                     /gene="htpX"
                     /locus_tag="WGR46_000023"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0855057.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSQGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26079..26639)
                     /locus_tag="WGR46_000024"
     CDS             complement(26079..26639)
                     /locus_tag="WGR46_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855058.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGFTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(26845..27237)
                     /locus_tag="WGR46_000025"
     CDS             complement(26845..27237)
                     /locus_tag="WGR46_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0855059.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(27354..27764)
                     /locus_tag="WGR46_000026"
     CDS             complement(27354..27764)
                     /locus_tag="WGR46_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0855060.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(27778..28215)
                     /locus_tag="WGR46_000027"
     CDS             complement(27778..28215)
                     /locus_tag="WGR46_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0855061.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(28628..29926)
                     /locus_tag="WGR46_000028"
     CDS             complement(28628..29926)
                     /locus_tag="WGR46_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0855062.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30029..31180)
                     /locus_tag="WGR46_000029"
     CDS             complement(30029..31180)
                     /locus_tag="WGR46_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0855063.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            31515..32894
                     /gene="norM"
                     /locus_tag="WGR46_000030"
     CDS             31515..32894
                     /gene="norM"
                     /locus_tag="WGR46_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0855064.1"
                     /translation="MLLDLNRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            32938..33978
                     /gene="murB"
                     /locus_tag="WGR46_000031"
     CDS             32938..33978
                     /gene="murB"
                     /locus_tag="WGR46_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0855065.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            33990..34640
                     /locus_tag="WGR46_000032"
     CDS             33990..34640
                     /locus_tag="WGR46_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0855066.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            34695..35471
                     /locus_tag="WGR46_000033"
     CDS             34695..35471
                     /locus_tag="WGR46_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0855067.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHSSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSSDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECDETALPDPQTESVRQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNILGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            35522..36088
                     /locus_tag="WGR46_000034"
     CDS             35522..36088
                     /locus_tag="WGR46_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0855068.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            36108..36998
                     /locus_tag="WGR46_000035"
     CDS             36108..36998
                     /locus_tag="WGR46_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0855069.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            37023..37781
                     /locus_tag="WGR46_000036"
     CDS             37023..37781
                     /locus_tag="WGR46_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0855070.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(37836..38501)
                     /locus_tag="WGR46_000037"
     CDS             complement(37836..38501)
                     /locus_tag="WGR46_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0855071.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(38615..39511)
                     /locus_tag="WGR46_000038"
     CDS             complement(38615..39511)
                     /locus_tag="WGR46_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0855072.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQYSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVCLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            39580..40737
                     /locus_tag="WGR46_000039"
     CDS             39580..40737
                     /locus_tag="WGR46_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0855073.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(40800..41801)
                     /gene="hemB"
                     /locus_tag="WGR46_000040"
     CDS             complement(40800..41801)
                     /gene="hemB"
                     /locus_tag="WGR46_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0855074.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(41984..42268)
                     /gene="yhbY"
                     /locus_tag="WGR46_000041"
     CDS             complement(41984..42268)
                     /gene="yhbY"
                     /locus_tag="WGR46_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0855075.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            42378..42998
                     /locus_tag="WGR46_000042"
     CDS             42378..42998
                     /locus_tag="WGR46_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0855076.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            43062..45029
                     /gene="ftsH"
                     /locus_tag="WGR46_000043"
     CDS             43062..45029
                     /gene="ftsH"
                     /locus_tag="WGR46_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0855077.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            45251..45682
                     /locus_tag="WGR46_000044"
     CDS             45251..45682
                     /locus_tag="WGR46_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0855078.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            45675..45953
                     /locus_tag="WGR46_000045"
     CDS             45675..45953
                     /locus_tag="WGR46_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0855079.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            46006..46584
                     /gene="pth"
                     /locus_tag="WGR46_000046"
     CDS             46006..46584
                     /gene="pth"
                     /locus_tag="WGR46_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0855080.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            46645..46851
                     /locus_tag="WGR46_000047"
     CDS             46645..46851
                     /locus_tag="WGR46_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0855081.1"
                     /translation="MTELKQLIQTESILVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            46848..47210
                     /locus_tag="WGR46_000048"
     CDS             46848..47210
                     /locus_tag="WGR46_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0855082.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            47441..48856
                     /locus_tag="WGR46_000049"
     CDS             47441..48856
                     /locus_tag="WGR46_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0855083.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            48975..49484
                     /locus_tag="WGR46_000050"
     CDS             48975..49484
                     /locus_tag="WGR46_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0855084.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNHRSKEMYGRTVVQDWGYAVFGKVVGGFDVVDAIEGVSTKRHGYHDDVPTEP
                     VIITKAEAV"
     gene            49647..51029
                     /locus_tag="WGR46_000051"
     CDS             49647..51029
                     /locus_tag="WGR46_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0855085.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSTMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGRRVEFPDGFGLMRTSNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(51049..51804)
                     /locus_tag="WGR46_000052"
     CDS             complement(51049..51804)
                     /locus_tag="WGR46_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855086.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(51814..52530)
                     /locus_tag="WGR46_000053"
     CDS             complement(51814..52530)
                     /locus_tag="WGR46_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0855087.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(52520..53347)
                     /locus_tag="WGR46_000054"
     CDS             complement(52520..53347)
                     /locus_tag="WGR46_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0855088.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            53632..54540
                     /locus_tag="WGR46_000055"
     CDS             53632..54540
                     /locus_tag="WGR46_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0855089.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            54773..58387
                     /gene="recB"
                     /locus_tag="WGR46_000056"
     CDS             54773..58387
                     /gene="recB"
                     /locus_tag="WGR46_000056"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0855090.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            58426..58785
                     /locus_tag="WGR46_000057"
     CDS             58426..58785
                     /locus_tag="WGR46_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0855091.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            59027..59506
                     /locus_tag="WGR46_000058"
     CDS             59027..59506
                     /locus_tag="WGR46_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0855092.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(59782..61161)
                     /gene="radA"
                     /locus_tag="WGR46_000059"
     CDS             complement(59782..61161)
                     /gene="radA"
                     /locus_tag="WGR46_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0855093.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(61350..62414)
                     /locus_tag="WGR46_000060"
     CDS             complement(61350..62414)
                     /locus_tag="WGR46_000060"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0855094.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(62489..63706)
                     /locus_tag="WGR46_000061"
     CDS             complement(62489..63706)
                     /locus_tag="WGR46_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0855095.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(63703..65037)
                     /locus_tag="WGR46_000062"
     CDS             complement(63703..65037)
                     /locus_tag="WGR46_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0855096.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(65052..65813)
                     /locus_tag="WGR46_000063"
     CDS             complement(65052..65813)
                     /locus_tag="WGR46_000063"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0855097.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(65831..66253)
                     /locus_tag="WGR46_000064"
     CDS             complement(65831..66253)
                     /locus_tag="WGR46_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855098.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(66504..66824)
                     /locus_tag="WGR46_000065"
     CDS             complement(66504..66824)
                     /locus_tag="WGR46_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0855099.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(66847..67473)
                     /gene="upp"
                     /locus_tag="WGR46_000066"
     CDS             complement(66847..67473)
                     /gene="upp"
                     /locus_tag="WGR46_000066"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0855100.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(67579..67890)
                     /gene="grxD"
                     /locus_tag="WGR46_000067"
     CDS             complement(67579..67890)
                     /gene="grxD"
                     /locus_tag="WGR46_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0855101.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(67991..68206)
                     /locus_tag="WGR46_000068"
     CDS             complement(67991..68206)
                     /locus_tag="WGR46_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0855102.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(68232..69011)
                     /locus_tag="WGR46_000069"
     CDS             complement(68232..69011)
                     /locus_tag="WGR46_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0855103.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(69016..69366)
                     /locus_tag="WGR46_000070"
     CDS             complement(69016..69366)
                     /locus_tag="WGR46_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0855104.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(69370..70347)
                     /locus_tag="WGR46_000071"
     CDS             complement(69370..70347)
                     /locus_tag="WGR46_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0855105.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(70381..71493)
                     /locus_tag="WGR46_000072"
     CDS             complement(70381..71493)
                     /locus_tag="WGR46_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0855106.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            71679..72380
                     /locus_tag="WGR46_000073"
     CDS             71679..72380
                     /locus_tag="WGR46_000073"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0855107.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            72524..74317
                     /gene="lepA"
                     /locus_tag="WGR46_000074"
     CDS             72524..74317
                     /gene="lepA"
                     /locus_tag="WGR46_000074"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0855108.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            74460..75479
                     /gene="lepB"
                     /locus_tag="WGR46_000075"
     CDS             74460..75479
                     /gene="lepB"
                     /locus_tag="WGR46_000075"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0855109.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            75577..75683
                     /locus_tag="WGR46_000076"
                     /pseudo
     CDS             75577..75683
                     /locus_tag="WGR46_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(75744..76643)
                     /locus_tag="WGR46_000077"
     CDS             complement(75744..76643)
                     /locus_tag="WGR46_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0855110.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(77209..78141)
                     /gene="cysK"
                     /locus_tag="WGR46_000078"
     CDS             complement(77209..78141)
                     /gene="cysK"
                     /locus_tag="WGR46_000078"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0855111.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(78335..78940)
                     /locus_tag="WGR46_000079"
     CDS             complement(78335..78940)
                     /locus_tag="WGR46_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855112.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(78937..79788)
                     /gene="dapF"
                     /locus_tag="WGR46_000080"
     CDS             complement(78937..79788)
                     /gene="dapF"
                     /locus_tag="WGR46_000080"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0855113.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIISRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            79925..80221
                     /locus_tag="WGR46_000081"
     CDS             79925..80221
                     /locus_tag="WGR46_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855114.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            80235..80903
                     /locus_tag="WGR46_000082"
     CDS             80235..80903
                     /locus_tag="WGR46_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0855115.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(80976..83096)
                     /gene="pnp"
                     /locus_tag="WGR46_000083"
     CDS             complement(80976..83096)
                     /gene="pnp"
                     /locus_tag="WGR46_000083"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0855116.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(83333..84196)
                     /locus_tag="WGR46_000084"
     CDS             complement(83333..84196)
                     /locus_tag="WGR46_000084"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0855117.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPTDQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            84395..85258
                     /gene="rfbD"
                     /locus_tag="WGR46_000085"
     CDS             84395..85258
                     /gene="rfbD"
                     /locus_tag="WGR46_000085"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0855118.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFIR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(85733..85966)
                     /locus_tag="WGR46_000086"
     CDS             complement(85733..85966)
                     /locus_tag="WGR46_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0855119.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(86132..86332)
                     /locus_tag="WGR46_000087"
     CDS             complement(86132..86332)
                     /locus_tag="WGR46_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0855120.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(86504..87379)
                     /gene="xerD"
                     /locus_tag="WGR46_000088"
     CDS             complement(86504..87379)
                     /gene="xerD"
                     /locus_tag="WGR46_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0855121.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(87431..87871)
                     /locus_tag="WGR46_000089"
     CDS             complement(87431..87871)
                     /locus_tag="WGR46_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0855122.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            88192..89130
                     /locus_tag="WGR46_000090"
     CDS             88192..89130
                     /locus_tag="WGR46_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0855123.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            89259..89552
                     /gene="hfq"
                     /locus_tag="WGR46_000091"
     CDS             89259..89552
                     /gene="hfq"
                     /locus_tag="WGR46_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0855124.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            89637..90203
                     /locus_tag="WGR46_000092"
     CDS             89637..90203
                     /locus_tag="WGR46_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0855125.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCHLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            90257..90631
                     /locus_tag="WGR46_000093"
     CDS             90257..90631
                     /locus_tag="WGR46_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0855126.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPKKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            90641..91135
                     /gene="folK"
                     /locus_tag="WGR46_000094"
     CDS             90641..91135
                     /gene="folK"
                     /locus_tag="WGR46_000094"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0855127.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(91263..91742)
                     /locus_tag="WGR46_000095"
     CDS             complement(91263..91742)
                     /locus_tag="WGR46_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0855128.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(91962..92699)
                     /gene="ubiE"
                     /locus_tag="WGR46_000096"
     CDS             complement(91962..92699)
                     /gene="ubiE"
                     /locus_tag="WGR46_000096"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0855129.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(92742..93110)
                     /locus_tag="WGR46_000097"
     CDS             complement(92742..93110)
                     /locus_tag="WGR46_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0855130.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            93230..94849
                     /gene="mnmC"
                     /locus_tag="WGR46_000098"
     CDS             93230..94849
                     /gene="mnmC"
                     /locus_tag="WGR46_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0855131.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPEINLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(94846..94995)
                     /locus_tag="WGR46_000099"
     CDS             complement(94846..94995)
                     /locus_tag="WGR46_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855132.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT        22797 a        26723 c        23187 g        22484 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcttt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgccacat cgaaatcctt
     2761 gggttcgatg ccgagcagca gatccctgat cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc agacggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcgggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggccgtctg aaacgcggga
     3661 atgttgtgtt cgagtatgct caagcccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaagtgaag attgggaatg
     3781 gaacgccccg aaccgcgcac ttcaccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcaacg atgccgacca cgccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt caggaaaagc attcagcgcg
     3961 tcttgcagca tagactgctc gatttcgcga cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaaggtcgcc cagattcggc
     4321 tctttgatgc cgtctgaaaa ataatcgcgg cggcgcaatt cggcacgcaa cgccgtcaac
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccaca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtatcggcag gcaaatggtg gtcggttaca
     4501 atgacatcca aacccaattc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tcaaccgtaa tcagcaaatc cacgccttgc gcggcggcga tttcggccag ctcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acgaggaaat ccactttcgc gcccatcgcc
     4681 gccaaaccgt ccaaaccgac ggcacacgcc gtcgcgccgt cggcatcata gtcggcgaca
     4741 atcaggattt tttcctgacg ctcaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcagcg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaggcaca aagccgggcg attaaaggat cggcgccggc ggtgagcaaa
     4921 tgattgaaaa cgtcggtatt gacggatcgg gtttggattt tgacggacat agcgtggaac
     4981 tttacagtgc ggatgtgttt gaaatgccgt ctgaaggctt ttcagacggc attgccgttc
     5041 atttgtctga ggaattcgtg gctgcaatca acgccgcgac aggcaaggcc agcaccgcgc
     5101 ccaccgcatc cagtatcaac gtggtgggcg tatatgcaat attttcaaac aacttggatt
     5161 tgtcggtatg ttttttcgta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 gataatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gagacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagttcga ccgcttcaaa ctcaagctgt ttcagcttgg
     5341 cgatgtcggc aggtctgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctggcggg cgattcgagt ttgaccggca gggcttggta ggcaaagcgc gggttgggct
     5461 caaccatttg aaactgcttg tccaacccgg ccttcaaaat gcccgtcagc ttcgccgaat
     5521 cttcaggatt gacgacgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
     5581 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtccttg tcaacgtgtt
     5641 tgcggtcggt cgtttggctg aacgggtcgt tcatacccca caacataaac gtacagccat
     5701 tcagcatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatcct
     5761 ccaatctaaa taacggcgga tgtcgtctga aaatatgcct aaatttccgc gtccccgttt
     5821 tccataacgg ttttcaattc ttgaaacagg gcgcggaaat ttttcggcgg tttgttttgc
     5881 tcctgctctt ttttggtgtt gcggatcaag gtacgcaatt taccggcgtc cgcatgcgga
     5941 aaatccgaca taaactgcgt caacgcgccg tcgtctgcca acagccgtac gcgcgcctgt
     6001 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaagaaac
     6061 gcctcgatgg gcgcgggatc cgtgtcgcgc atcagcctgc cgataaattg cgcctgccgc
     6121 ttgagcgcgc cgttggatgt gattttttta taggcggtta ccgcctcgta caaatccgca
     6181 tccaaaccga tttttttcag cgtatcgttt gagagcttgg tcaacgccat acccaaatcc
     6241 tgcaaatcgt tcatctgctt tttcatttgg gttttgctga tccattcgtc ttcttgttca
     6301 aacatcttga ttctcgaatt tttccaatag cgcattttaa cagaatgccg ccgcgttcag
     6361 acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt tgcccctgcc gcccgaaacg
     6421 gttaaaatgc cgcaaagcat catcaagaaa gatttctatg ctgttcaacc acaccgcttc
     6481 cgaactcctc gacctctgcc gccgcacgct cgacttggca aaagcagcgg gcgcgaccgc
     6541 cgccgaagcc gatttcagcg aatccatcgg acaaagcgtc agcgtgcggc tgggcgaaat
     6601 cgaacaaatc gagttccagc aggacaagtc gctggacatt accgtttacg tcggcaaacg
     6661 caaaggccgc gctggcaccg ccgatttctc cgaacaagcc ctgcgcgaca ccgtccgcgc
     6721 cgccgccgac atcgcccgcc acaccgccga agacggttgc gccggacttg ccgaccccga
     6781 gttgatggcg caacacatcg gcgaccccga cctttaccac gaatgggatt tggatacgga
     6841 agccgccgtc ggcttggcaa aacaatgcga acaagccgcc ctgaacgagg atgagcgcat
     6901 cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac caatacgttt acggcaacac
     6961 ccacggtttt gccgcacacc ggcaaggcac gcaccacagc atttcctgca gcgtcgttgc
     7021 cgccgacgaa aacgggatgc agcgcgacta ctggtacgat tccgcctgcc gccatccgga
     7081 tatggacagc cccgaaacca tcggtcaaac cgccgcccgc cgcactttgc gccgactcgg
     7141 cagccgcagc atcccgacag gcagctaccc cgtcctcttc gataccacca tttcgggcgg
     7201 tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg ctctaccgcc aaagcagttt
     7261 cctgatcgac agcatcggca aaaaagtcct gcccgatttc ctcaacctgc gcgaagaacc
     7321 gcacatcccc cgctctttcc gcagcagcta ttttgatgcg gaaggcgttg ccaccgcacc
     7381 gcgcttcgtg attcaaaacg gcattgtcga aggctatttc ctcagcagtt acagcgcgcg
     7441 caaactcggt atgcagacca cgggcaacgc cggcggcgcg cacaacctgt atttgaacca
     7501 tacgcacgaa acgcaatccg acctgctgaa agaaatgggg acgggattat tggttaccga
     7561 actgatggga cagggcgcga acaccattac cggtgactac tcgcgcggcg cggcgggttt
     7621 ttgggtggaa aacggcgtga ttgcctaccc cgtccacgag attaccgtag ccggacgctt
     7681 gcaggatatg taccgcgaca ttgtcggcgt ggcggatgac gctttgcggc gttcgtccaa
     7741 caaaatcggc tcgattctga ttgcggggat gacggttgcc ggaagctgaa cgccctgccg
     7801 atgccgtctg aagacgaagg cagcacgttt tgccccgtgc ctacccgttt gccgttttat
     7861 gccgcaccgc cttttgccga caacattgca cattttttca tcagcactga ttttccctta
     7921 tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca ggataaggcg
     7981 tgcggggatt ggggtttttc cgccgtcggc aaaacggcag acaaaaacgg cgggacattg
     8041 cctgtcccgc cgttttcaaa tatacatttg accgtacatc aggaattaag cagcctgaat
     8101 gttggcggcc tgtttgcctt tagggccggt ggttacgtcg aaagagacgc gttggccttc
     8161 tttcagggtt ttgaaacctt ccatattgat tgctgagaag tgagcgaaca aatcttcgcc
     8221 gccttcatca ggagtgatga aaccaaaacc tttagcgtcg ttaaaccatt ttacgatacc
     8281 ggttgccatt agaaacttcc tatactcaaa aattaacaaa atcagcaaaa caaggcatac
     8341 ggcaaaccga gccgtctggc gttccttcca gcttgatata ctcggcggat gcccaatcct
     8401 gcttaaccgt ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg
     8461 ggaaaatagg caaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa
     8521 tgggacacac tatgaccgcg caacaccaat ccgacacgct cttgcaccgt ctgaacaccc
     8581 tgccgccgaa acgttacggc gttttcctat tgaacgacga ttacaccacg atggaatttg
     8641 tcgtcgaaat cctgaccgaa atctttatgc tcggacaaga acaggcggta gcggtgatgc
     8701 tcttggttca tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat attgcccaaa
     8761 ccaaacaaca acaagtcatg cagcgggcaa aagccgaagg gcatccgctg caatgtattg
     8821 tcgaggagat ttaatatgct tgcacccgaa ttggaacaga ttttgcagca gctttaccgc
     8881 gaggcgcgta aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc
     8941 gaagaagatg cctccgtgcc gaacgtctta aaactatgcg gcgcggattt gaaagtggtg
     9001 tccgaacagc tcgccgccag cgttgccgaa aacacccccc tgattcccga acacctttta
     9061 gacacggtcg aaacccggcc cacgctcggc ttccaacgcg tgatgcaacg ggcgatggtg
     9121 catacccagt ctgccggaaa agccgcagtc gaaccgttgg acgttttggt cgcgctgatg
     9181 agcgaaaccg acagccacac cgtctatttc ctcaagctgc aatcggttac gcgttttgaa
     9241 gttttgcgct gtattgccca tggctcgccc gatgacgagg atggcaatga ttcagacggc
     9301 ctgggacgcg agggcgaaga agcggaacaa aaaaccggct ccctttccga ctacaccgtc
     9361 aacctcaaca ccgaagtcaa agccggccgt atcgaccctt tgattggtcg caaacacgaa
     9421 atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct tttggtcggc
     9481 gaagcaggcg tgggcaaaac cgcgctggcg gaaggtttgg cacatcaaat cgtcaacggc
     9541 ggcattccag acgcgcttaa agatgccgaa gtgtacgcgc tggatatggg ctcgctgttg
     9601 gcgggcacga aataccgtgg cgactttgaa gcgcgggtca aatccgtctt gaaacagctc
     9661 gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat cggcgcaggc
     9721 agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct ggcaaaaggt
     9781 tcgctgcgct gcatcggcgc aaccacctac gacgaatacc gcaccatttt cgacaaagac
     9841 tatgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt ttccgaaacc
     9901 gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt ccgctatacg
     9961 caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga gcgtttcctg
    10021 cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat tctgcccaaa
    10081 tccaaacaga aaaaagtcat cggcaaagcg caaatcgaaa ccgtcatcgc caaagtcgcg
    10141 cggattccag aaaaaaccgt gtcgcacgac gacaaacagg tgctgcaatt cctcggccgc
    10201 gatttgaaaa acatggttta cggtcaagaa aacgccatcg acgcgctggt tgccgccgtc
    10261 aaaatgtcgc gttcgggctt gggactgccc gacaaaccga taggcagctt cctcttctcc
    10321 ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc ttgcctactc gatgggcgta
    10381 ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc gcgcctcatc
    10441 ggcgcgcctc cgggctacgt cggcttcgaa caaggcggcc ttttgaccga agccgtcaac
    10501 aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc cgacattttc
    10561 aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg caagagtgcc
    10621 gatttccgca acgtcatcct gattatgacc accaacgcgg gcgcggaaag cctcagccgc
    10681 cccggcctcg gctttaccgc caagcgcgaa cgcggcgacg aaatgcaggc catcaacaaa
    10741 ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccgtttgc acccctgtcc
    10801 gaacccatta tcgccaaagt cgtggacaaa ttcctgctcc ggctcgaaca ccggctcctc
    10861 gacaagaaag tcgaagccga attcacaccg gcattgcgca aatatctggc gagaaaaggt
    10921 ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga ttcaggaaaa aatccgcaaa
    10981 ccgctcgccg acgaactcct gttcggcaaa ctatccaacg gcggcttcgt gcggatagac
    11041 tgggatgcgg caaaagaaga agccgtgttg aaatttaaga aaagcaaggt taaacctgaa
    11101 acagaaactg tttaaccgat tgaaaaataa aatgccgtct gaaatttcag acggcatttt
    11161 tttaaatatc tgtccctatt ccggcaaact tttccacaaa cgccgcaatc ttttgaaaaa
    11221 cactttgttt cttcgtcaga tattgcgggt tgagcgggct cattttcggc agggtttcgg
    11281 tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc aaataacgtt
    11341 tggcggcggt ttcgttcagg tcttcggcgg caatcagctc tgctgcttcg cgcctcatca
    11401 cctcttgagc gtaggtgtaa aaggtttcca gtatggcagg aacgtcgggg actttgtcca
    11461 aatccgtatc gttgatgaaa tccacaatca ggctctcttt ggcgcggtgg ccgatgctgg
    11521 cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt tttttgtggt
    11581 gctcgaaaac cagttgcaag atgtaatcca gattgatttc ctgcgatttg agcaaatcca
    11641 cctcaaaaac cacatcgtcc caatcgattt ttgattgttc tttctgttcg cctgcttttt
    11701 ggcggcgcag ccagtcgcgg atgtcgttat aggcagaacg gtagtcctgc actgcccttt
    11761 cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcagg tagtattttt
    11821 cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac tcgcgcagcg
    11881 cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg ccgaagagtt
    11941 tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg aaacgctcgc
    12001 gcaattcttt tgccacatcc aaataaccgc gccgcacttc gcccgtccga tcatcgatat
    12061 agccgttcat gtattcttcg tagctttttt ccaacaccac gtttttggtg tttttatcgc
    12121 cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag acaatattgc
    12181 cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc atcaggccgt
    12241 gatagcgcag gtttttatcg acaaacagcg tgttcagcgt cggcgcgtca aaacccgtca
    12301 aaaacatacc gaccacaatc agcaaatcca cttcctggtt tttcacccgt tttgccaaat
    12361 cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg aaacaggcgt
    12421 tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc gcttccggtt
    12481 caaaggtctc atcaacaatt tcaccgacgg cgttctgctc ttcgttggcc gcaaaggaaa
    12541 aaatggtggc cactttcagc gggtgcaggc tgcctgcctg ttgtgttttg aacgcttcgt
    12601 aatagcactt cgccgcatcc acgctgctga ccgcaaacat ggcgttgaag cctttaccgc
    12661 ccgcattcag gcggtgcgtt ttctgcctga actggttcag gatatattgc gtgatttcgc
    12721 ggatgcgttc ggggtgcagc agggcttggc ggttttcggc agcactcagt ttcttctcat
    12781 cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc actttgaatt
    12841 tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcaac tcacgcccga
    12901 acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc gtaccggtaa
    12961 aaccgaactg gcagaatttc ttaaatttct ttttcaggtt ttttgcgctt cgccgaattg
    13021 cgagcggtgg cattcgtcga aaataaatac aacctgctga tggtaaaccg gcagattatc
    13081 ttcactcttc atcaggttgt tcagcttttg gatggtggta acgatgattt tgttgtcgtc
    13141 tttttccaaa ttgcgtttca agcccgccgt gctttccgaa ccattcacgc tgtcgggcga
    13201 aaaacgttgg tattccttca tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa
    13261 aaccttgtcg ataaacgccg attccgtcgc cagacgcgcc gccttaaagc tggtcagcgt
    13321 tttgccgctg cccgtggtat gccaaacata gccgccgctt tccggtttgc tccaattctt
    13381 tacctacgcc gagctgttga ttttccacaa aatgcgttcg gcggcggcaa tctgatacgg
    13441 ccgcataatc agcagcgtat cattcgcatc gaacacgctg taatgcagca atacgcccag
    13501 caatacgctt ttctgcagga acgtggcggt aaagtctttc aaatccttaa tcggatgatt
    13561 gtccgaccgc gcccaattca tcgtgaaatc aaagctgttt ttgtcgcgct tggtggtgtt
    13621 ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag atttgcagga atttgaacag
    13681 cgaattttcg ctgttgaagc tctctttgct gtaacggtgc acctgattga atgcttcgcg
    13741 caccgccacg ccgcgctttt tcaattcaat ctgcaccagc ggcaggccgt ttaccagcac
    13801 ggtaacgtca tagcggtttg catacgtgcc cgtctgctca aactgattga taatctgcac
    13861 atggttgcgg gcaaggtttt tcttgtccag cagataaatg tttttcagac ggccgtcatc
    13921 aaaagcgaaa tcataaatat ggtcgtcatg gattttgcgg gttttatcgg taatgttttc
    13981 agacggcctg tccagatatt ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac
    14041 atcgttcagc cgctgcaact gcgcgcgcag gttttccagc agcctgcttt ggctgttcaa
    14101 atccttgcgg tattcgtaac cctgattctg caaatcggcg attaactccg cttccaactg
    14161 gttttccgat tggtagctgc ccgactgttc gattttttca tattggtcga gcacgatgaa
    14221 attcggcgtt tcggcgatgg gtttggtttc gaggttcatg ggtcgtcctt tgcagtatcg
    14281 ggttttaaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc tgctttttca
    14341 ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag
    14401 ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc agggtatcga atttgtctag
    14461 gatggcggca attttttcct gttcggggag tggtgggatg gggattttta atttctttaa
    14521 ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc tctgctccgc
    14581 aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa attcatcttt
    14641 aattgtaaaa ttggtcagtt gttggttact aatataatta acttttatta acgcatgctc
    14701 tcctatggtt gccgttgtcg acatcattat tgatttagca ggaaacagct tgccgccctt
    14761 aactgcgctt tttgatatat gcttcaatga attatctaaa atacggctat tttccctaat
    14821 atcttccatt ctaaaccaag ggattgagcc attttcccaa tattctttat ttgatttgga
    14881 aggggtatag ccattcttca agtcaaacac ctcccccaac gtcttccaaa ccacatcttt
    14941 cagacggcct ttatagccat cagctatccc ccccccgatt tgattgtcaa aatctaaaag
    15001 aaagtcgcgg taataccggt attggcgttt gcgcagggct aattcagctt ccagctcggt
    15061 gaatttgtca agtattttta caattttttg ttgggtttcc aggggtggga tagggattaa
    15121 taattttttc aagttactgg catttagagc aggaatacct gtaaatgtac ataaggcatg
    15181 aatctcttgt tcaaattcaa gtaatatatg atagagaaaa cgaacatcca attctgttct
    15241 attcttaata gtaactgcat aatttaagtt gccacgaaaa tatcttcctt cttgccacgt
    15301 tattgagccg actcccgctc ctcttgttgt gatgccgata gggtcatttt cagtgttcca
    15361 ttcatcaata tagccaagcg gctctttgcc actgttaata actggataac taccaatatt
    15421 atttgagatt ttctgcttac ttacagcttg tcctgttttt atgatagcta tcccattttc
    15481 tccacccaac ggcttccact ccaccggtgc cgtctgaatc atctcaatca attttttcgc
    15541 tttgctttgc atatccatgg ttgttttccg tttctttccc gctgtggcgg gatgtggggc
    15601 ggttgtgggg tgcttcggct cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc
    15661 gtcctgcttt caggctgcct gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg
    15721 tagttatttt gtgcggtctt ttttctgttt gatctgttgc acggctgttt tttccaacgc
    15781 tttaacgctt tccagatagg cttgctccac cggcgacagg atgcgcgctt cataggcgcg
    15841 gtattcgcgt tcggctttct gctctgcctg tttgcggctg atgctgcctg caccctgtaa
    15901 tgtgccttgt ccgtacagcc cggaaaattt gtccaattcg gctatccagt cgcgcatata
    15961 catggggctt tgctcctgcg ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg
    16021 gttcaggcgg aacagttcgt cttcggtcag atagtttttg gcgattttgg cttcattcag
    16081 cgtggggatt gcgccttgaa aggtggtcag ccccataaag tctttgctgc tgtcggcacg
    16141 gctgtatatc agctcagctg cggtttgccg gctggcggca tagtgcagtt tgttttgaac
    16201 ggcggcaaaa aaggtttggc tttcgctgct tttggggttg tagtcttggc tggtggcata
    16261 taaatcaagc acttgccggt atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt
    16321 gagcagttct ttccaatagt cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc
    16381 aaagcctttg gtcagatatt cgtccagccg ttcggttgcc cattggcgga attggatgcc
    16441 ccgcgcggaa cggacgcggt agccgacggc aataatcatg ggcagggaat aatgggcgat
    16501 tttgcggttt accttgcgcc cgttttcatt ttgaactgtc aactggaagt tgacagttgc
    16561 cttctcttcc aattcttgct ctgcaagaat ggttttaatg tgtttgctga tattttgttt
    16621 ggtggtttgg tacagttctg ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc
    16681 ctgcaaggca aattgggcag tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca
    16741 atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt cgattttggc aacggtttcg
    16801 ctgatttcgg cgttaagctg tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca
    16861 tagctgctga cggcgaggtt gtagccgttg tctttgacgg tttgctgggc ggcgttttgg
    16921 gcgatatgcg gcacatcggc tttatcggcg aagagtttga cgatttcggc aatgtgttct
    16981 tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg
    17041 atgtcggtat tgtctttgtg tttggacaaa accagtatat tgacggcgat gccggtgccg
    17101 taaaagagat tgggcgcaag ggcgatgacg gtttccacgt agttgccctc caccagatat
    17161 tggcggattt tctgctctgc gccgccgcga tagaaaatgc cggggaatga gacgatggcg
    17221 gcgcggcctc tgccggaaag gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg
    17281 gatttcgggg cgagtacgcc tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg
    17341 ccgctgccta tccagttgat ggaatagggc ggattggaaa cgacggcatc aaagggtttg
    17401 ctgtctttga gtttgggatt ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa
    17461 ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc
    17521 tgcccgaaga agccttcttc gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc
    17581 aggctgcccg agccgcaagc tgggtcgtag attttgttta ctttctcctg cccgtgcacc
    17641 gccagccgcg caatcagctt ggatacgctt tgcggggtga aaaattcgcc gccggatttg
    17701 cctgcgttgg cagcgtagtt ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg
    17761 tggtagtctt caaaactgcc gaaatcgagt tccgccacgc cttttaggac ggcggcaagg
    17821 cgtttgttct tgtcggcaac ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca
    17881 aacaggcctt taatgtcttg ttcggacgga tagccggagg cggagctttc aatcgcggta
    17941 aaaatttctt tcagcttggt gttgagctct tcgttttgat gggcttcggc ggcaatattg
    18001 caaaaaagct ggccggggta gatgaaatag cctttgactt tgacggcatc gtctttgatt
    18061 tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc
    18121 atatagtcgg taaagttttc gctgataaag cggtagaaaa gtgtgccgag aacgtattgt
    18181 ttaaagtccc agccgtccac cgcgccgcgt acttcgtcgg caattttcca aatttggcgg
    18241 tgcagttggg cgcgttgttg catttcggtc atcatcgaaa tccatataaa agttaaacaa
    18301 atcgaaatcg cctgatattt tcagacgatt ttttacgggc attcaaatta agccaaattt
    18361 tccagcatcc atttgacgta gcgctcgacg cctgctttga catcgaaaaa ttcttccgta
    18421 taacctgcct cgcgcaattt ggtgatgtcg gcttgggtga agctttggta tttgcctttg
    18481 agcgcgtcgg gaaatgggat gtagcggata aattcttctt ctaccaactc ttttaaactc
    18541 atttcaggtt tgccttcggc ggcgcggcag gcgttgacgg tggcagcggc gagttcgtta
    18601 aactgttggc tgcgacctgt tcctaggttg tagatgccgg aaagttcggg atggtcgaag
    18661 aagtagaggt tgactttggc gacgtcttcg acgctgacga agtcgcgggt ttgttcgccg
    18721 ttgccgtagc catcgttgct gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg
    18781 aaatggtgga aggcgacgga tgccatgcga cctttgtgtt gttcgtgttg tccgtaaaca
    18841 ttgaagtagc ggaagccgac gacttgggcg gtcagccttt ctttcatgcg gcggcgcaat
    18901 acttggtcaa acaggaattt ggaatagccg tacacattaa gcggtttttc cagttcgcgc
    18961 tcttcgcgga agatttcgcc tttgccgtaa accgccgcac tggaggcata aaggaaggga
    19021 atgcgttcgt cctgacacca gtccaacaaa tccagcgtgt actggtagtt gttttccatc
    19081 atatacaaac cgtcgtgatt catggtgtcg gaacacgcgc cttgatggaa aacggcttcg
    19141 atgttttgat aaggtaaaat gtgttccctc acttggcgga tgaattcatg tttgtcgaga
    19201 taatgggcga tttcgcactc ggcaaggttt ttgaattttt cgcctttgct caaattatcg
    19261 acggcaacaa tgtcagtaat accgcgttga ttaagtgctt tgacgatgtt gctgccgata
    19321 aagccggccg cgcctgttac gatgatggtc atattcggtt tcctttgttt tgagtaaaat
    19381 taataaactt agttacaaga aatcatcgat attgtcctga aacacaaatt tgcgatgata
    19441 ttccaaatat ttttctcttc cttcgggata tattctcatc ttattttttg catttggatt
    19501 gatattaagt ttttcccatg tgtaacggct tagtcgagta ccgcaaatca aacgaccatc
    19561 atccaagaaa gtaatataac cttgatcgaa caaccaatca taagtcggcg ttaaagctaa
    19621 cccatttaaa taatctaatg cctctttctc atttttctca gtaatacaaa ccatataagg
    19681 tttaatatga ctagccctta acaaaatctc atctgtaatt aatgtaaatg gacattgcgg
    19741 catatgattt ataacatcct tgcgatattt ctgctgccct tttctacttt tctgtttaat
    19801 gatttcttgc tctacgaatt gattaaattc tgaagccggt atttctaatt tttctcttga
    19861 taagagttgc gggtgcacga tagagcgaaa ttgataatcc aaaaatattc ggaaataaaa
    19921 taatggttct gaatcttcta tatctttgac aggcaatagt ttaagaattg ataaataact
    19981 aattttgggc aatacaatct tacgccaaat tgaccatata tcatcctccg aacggatata
    20041 acccctacgg taacctttag aattttccaa atggtctgat acatcatgga ttgaaaagaa
    20101 aatattttct gtaggcagat tattaatact taaaacgtaa tcatcataaa accttgaaat
    20161 atcatgatga tatttctgtt cttgtttgtg atattcaact tttgcatcaa ccaaataacg
    20221 ctgtaaattt ttcttattaa aaaaacaatt tttctgaatg agaggatatt ctaccctcct
    20281 tccatttttt gtctcagtaa tcccccaatt ttcatactca aaaaatgtac ttagttttcg
    20341 accactatca tcaatataac tacctacata tttgcgtgct tccccattac caccaaaaac
    20401 agataacttg ttattcctgt gaataaaact atcttccgca cgtaaatttt gaatactatc
    20461 gacaataaaa tactctatgt tttcaatagt aattctttga aaatccggca ttttactctc
    20521 cataaagcgt aatatccatt tgttttaata tagcgattaa tacatcaaca acaatactgt
    20581 ttcccgcttg gcgatacatt tgagtatcac taactaaaat tttaaaatca tctctaaaac
    20641 ccattagccg caaacattcc ctaggtgtta atttacgaat acgtccacga ttatgagtaa
    20701 cataattatc aaccccggca cgatgcattt tatgcatagt ttgcaataat gggcgagcta
    20761 caggcaaatc agtttccgta ctggttttaa aattttttgt tcctcctgcc aaaacataat
    20821 ttttgatttt ttcagaaaga taatattttt cctcaacgtt atttacatca aaaataaagt
    20881 catcaaattc agattcgcgg gctgcctgaa aaataaaatc accatgccaa ttaaattgtt
    20941 gatttgcttt ttggcataag gcaatttctc cattaatctg tgtataacgt ttttgtctat
    21001 ttttagaact ggtaacaaat ttcacaccct tttcacgtaa aaaatatttg ctatcagtat
    21061 agtcctccaa aaaatcttgc atagtatgtt ctaattcaat cttttcagga aactgaaaac
    21121 catttatagg aggggtatga aagccaacaa caaaaatacg ctcacgatgt tgaggaatcc
    21181 cataatcctt actattcatt atttggaaat ataagtcata accaagtgaa taaaaaacac
    21241 tttttacaac tttccaagtt tttccattat catgattgag caagcccttt acattttcat
    21301 aaataaatat ctttggttgg acttcatcca caacgcgagc aaattcatag aataatgttc
    21361 ctcgtgtatc ttctaatcct gcacgtttgc caaccatgga aaatgcttgg caaggactgc
    21421 ctccaactaa aatatcaact tgatttctaa actttctcgc atcaaattga gtaatgtcgt
    21481 tatgccaaaa atcttcattt aatttatagt ttccaagata acttttttta acgtatggat
    21541 caatatctcc tgcaaaaaca atggtatggt ttaaatttaa tcggtggaat gcctgttcaa
    21601 ccgcaccaat cccgctgaat acggttgcta atctaatatg tgaatcaggt ttaagaaaag
    21661 ttttagattt ccaaccttgt tgactgggaa agagcaaagt tttttgtaat cgagtatcgt
    21721 gtgtctgtgc cattgtcgaa atagtcatac ttatatcgtt ctgtttatct tatcaatatg
    21781 aaaactacat tgttgattgc cctgacaatg ccttgatcaa ttcggcaaac gagcaaaccg
    21841 ccgtaccgag tttcgccacg acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg
    21901 gcatggtgca gcctgccgcc aaacccaagc ccattccggc aatgacggtg tcgcccgcac
    21961 cggatacgtc gtaaacttct tgggcgcggg tgggctggta aatcggttcg ccttcgctga
    22021 acaaggtcat gccttcttcg cttcgggtca gtaaaacggc ggtcaggtcg aggtggcggc
    22081 gcaggttttg cgctttttcg gtcagctcgc tttcgttttt ccaactgccg accacttctt
    22141 tcaattcggc gcggttaggc gtaatcagag ttgcaccgac atatttttcg taatcgtcgc
    22201 ctttggggtc gattaagacg gttttgccgg cgtgtttcgc ccaatcgatc atatcggaga
    22261 tatgcgacag gccgcctttg ccgtagtctg aaaaaatgat tgcgtcgtat tcgggcaaga
    22321 tttcgcggta tttctgcttg atttgttcca acacttcgca gttgggatgt tcttcaaaat
    22381 caagacggat aagctgctgg ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt
    22441 gtttgtcgcg catcagatag gaggcgacgc cgtcctgcac catcagcgca tcgagcgcgt
    22501 cggcggcttc gtcgttgccg gttacggaca acagccctgc cctgccgccc aacgaagcga
    22561 tgttgcgcgc gacatttgcc gctccgcccg cgcgttggtc gattcgtccg attttcgcca
    22621 ccggcacggg ggcttcgggc gaaatacggg acacatcgcc gaaccaatag cggtcgagca
    22681 tcacgtcgcc gacaaccagg actttggctt gcgcgaaacg ggatttgagg gtttcttgtt
    22741 ggaacttggc ggacatattg cctccgttgt attttttcag acggcctcag cctgtaaggc
    22801 cgtctgaaaa tcagtttgct tcaaggtttg ccacgcggtt cacttcgcgc aatacggcta
    22861 ccggatcggc agcttgggtt acaggacgac ccattaccaa ataagtcgaa gcggcagcca
    22921 aggcttcggc cggtgtcatg atgcggcgct ggtcatcatt attgccggca acgtccaagc
    22981 ggatgccggg cgtgaccaag acaaaatctt gtcccaactc gcggcgcagc ggtgcggctt
    23041 cttgggcgga acaaaccacg ccgtccaagc ccgaactttg cgccagtttt gccaagcgga
    23101 tgacttgttc ttcaggggcg gtgttcaaac cgatttccgc caaatcgctt tgttccatgc
    23161 tggtcaacac ggttacgccg attaagagcg gctttgtacc gtatccggca acggcttctg
    23221 ctgcggcttc catcatacgg cggccgcccg atgcgtgcat atcgaccatc caaacgccca
    23281 tatcggcagc gactttgcag gcttgcgcga cggtgtgggg aatatcgtgg tatttcaaat
    23341 cgaggaaaag tttgaaacct tgattgatta agctttctgc caaattgcgt cccgtcgcgg
    23401 taaacagctc tttgccgatt ttgatttgac acaatgtcgg gtcaaggttg cggacgaatc
    23461 cgagcgtgtc tttttcgttg gaaaaatcaa gggcgacgat gacgggggtg cgttgttgcg
    23521 gagtttggaa gtcggagatt aaaggattca tggtgttttc gtttcgggtt ttcagacgac
    23581 ctttatcgtc tttttcgcag tatagtggat taactttaaa ccggtacggc gttgtctcgc
    23641 cttagttcaa agagaacgct tctctaaggt gctgaagcac aaagtgaatc ggttccgtac
    23701 tatctgtact gcctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataacaa
    23761 cgtaaattca aaccgtaggt cgggcattga tgtccgacct acgcggactc aagcctccga
    23821 caaactgttt cggataaatt tgaaccggta catcagccgt cattcccgcg caggcgggaa
    23881 tccattgata aaactcagtt catcagattt aaacagcgat tgcccaaatt cagaaatgga
    23941 ttcccgcctg cgcgggaatg acgccaaatg attatttttc ggttgcaaca cgctgtttat
    24001 caaaaacagg tcgtctgaaa acctgatttt ctgttttcag agtgcgaagc ctgcccgtgg
    24061 gtacgacctt ttcgatttat ttgcccaatg cgcccaatac ttcggctttc acggcttcta
    24121 ccggctgagt gccgtcaact ttgatgtatt taggcgcgtg ttcgccttcc agtttgctgt
    24181 aaaaatcgac caaaacttcg gtttgctcgt ggtaaacggc aaggcgtttt ttcacggttt
    24241 cttctttgtc gtcgtcgcgc tgaatcaaat cttcgccggt tacgtcgtct ttgccttcaa
    24301 ctttgggcgg gttgtaggta acgtggtaag tacggccgga agccaaatgc acgcggcggc
    24361 cgctcatacg gtcgacaatc acgctgtcag gcacgtcgat ttcaacgact gcatccaaat
    24421 ccacgcctgc ttcaaccatc gcttcggctt gtgccaatgt gcgcgggaaa ccgtcgaaca
    24481 ggaaaccgtt tttgcagtcg tcttgcgcga tgcgttcttt gaccatgccg ataatgatgt
    24541 cgtcgcgcac caagccgcct tcgtcaatga tttttttcgc ttccaaaccc aagggcgtgc
    24601 cagccttaat cgcggcgcgg agcatgtcgc cggtagagat ttgcggaatg ccgaacgcgg
    24661 cggtgatgaa ttgcgcctga gtgcctttgc ccgcgcccgg cgcgcctaaa agtaatgctt
    24721 tcatgaagtg atcctttgaa atgtgtttat tggaatcaaa cagatggttt acgattctac
    24781 acggcttggc acaatagttc aaaaaccaac tgcacaaact gccatttgca agcaaatgtt
    24841 ttgcatacct ttgccgagtc tgatattcac aagccggcaa ctttttggga caatccccaa
    24901 aatttcaaaa catcacaccc taccaaaaag gaacatccgt gaaacgaatt tttctgtttt
    24961 tggctaccaa tatcgctgtt ttggtcgtaa tcaacattgt tttggcggtt ttgggcatca
    25021 acagccaggg cggcacgggc agcctgttgg cgtattccgc cgtcgtcggc ttcactggtt
    25081 cgattatttc gctgctgatg tccaaattca tcgccaaaca atcggtcggc gcggaagtta
    25141 tcgacacgcc gcgcaccgaa gaagaagcct ggcttttgaa cactgtcgaa gcccaagcgc
    25201 ggcaatggaa cctgaaaacg cccgaagtcg ccatctacca ctcccccgaa cccaatgcct
    25261 ttgccacggg cgcatcgaga aacagctccc tgatcgccgt cagcaccggt ttgctcgacc
    25321 atatgacgcg tgacgaagtg gaagccgtat tggcgcacga aatggcacac gtcggcaacg
    25381 gcgatatggt tacgctgacg ctgattcaag gcgtggtcaa tacctttgtc gtgttcctgt
    25441 cgcgcattat tgccaacctg attgcccgaa acaacgacgg cagccagtcc cagggaactt
    25501 atttcctggt cagcatggta ttccaaatcc tgttcggctt ccttgccagc ttaattgtca
    25561 tgtggttcag ccgacaacgc gaataccgcg ccgacgcggg cgcggcaaaa ctggtcggcg
    25621 cgccgaaaat gatttccgcc ctgcaaaggc ttaaaggcaa cccggtcgat ttgcccgaag
    25681 aaatgaacgc aatgggcatc gccggagata cgcgcgactc cctgctcagc acccaccctt
    25741 cgctggacaa ccgaatcgcc cgcctcaaat cgctttaaac cgatttgaaa cggcaaaaac
    25801 cgtacgcgca agcagtacgg ttttttgtat atgctgtctg aaaacatcag gtttccatac
    25861 cgacaccaac cgtatccatt tccgcaccgg atacgccgca ttgatatacc ctgataaacc
    25921 gtatccggac agaaaatata gtggattaac aaaaaccagt acggcgttgc ctcgccttag
    25981 ctcaaagaaa acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt
    26041 atactgtctg cggcttcgtc gccttgtcct gatttttgtt aatcccctat atcagataaa
    26101 acagcggtaa aattgccgtc tgaaaccaac tctgcgccat acgggacacg ctctgcacgt
    26161 ctatttgcct ccgatatatc ctccctactg taggaacgtc tgccgatttg cacgaaatcg
    26221 gcacctctag cacccttcag gatggcggac gttatccgtt cttgggattc tgcaagacgg
    26281 tcgacggcat tatacagacg ctcggtctga tacccttccg aagcaacctg cttcaggtat
    26341 tccaaataac cgtaacccaa cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt
    26401 gccgaacgga ttttaggggg catatcttga agcatatctc taccgaaccg aaccaaagtc
    26461 tctttaaagt cggactctac acgcgtaaaa ccctcggaaa tcttgaagtt caactcatac
    26521 tgcttccgtt tgtccgctgt caaccgcttg aggttgtcat ccccgtattg gacggttgac
    26581 acaaccctgt acagactgtc ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata
    26641 actggattcc caacccttgc cgccgattct gatactcaaa acggtatcca ggcgttccag
    26701 cagggcttcg ggagacagca gtgcgtattc ttcaatattt tccataggtt gattatagca
    26761 gaaccttata cctgttacag acaaggttgt cgggatttga acgtccgtcg agcttggttc
    26821 gtaggattta gggtgttgtg aaccttacct atcgtaccca agcactttgt acctgtttga
    26881 aataaggttg tcgggcgacg ttcgaccctt gagcctgtga ttgatttgtt ggaaatgtac
    26941 acctaaagca gcacctgcgg cagtaacgct tgggaacgtg agtgtttcgt tggtcgttgt
    27001 gtcgaggatg aggaggggct tccctacatt tggaggtgtt tgggtttttg acatcttagc
    27061 acactcagga cacccgtact ttgactttat aagggcatta aatgtcgaat aacgaacgtt
    27121 cccgtgcata ggacaattaa cggttactgg gtaattcaca ccgtcaaagt cggttagaga
    27181 gatattaggg aatatatcag taagttcaga agctgcttgg ttgaaagatt tggccatttg
    27241 agactccaaa attgattgtc aaatggattt aatagcaaat ttataaaaat gtcaaatttt
    27301 tcgagaagca cctgatagtg gcgaggggta tacactgtta aaaagtgtat acgtcattta
    27361 gtgcagatgt gaaccttata cctccctgca agtaaagtct ctgtgtgaac ccgacctttt
    27421 atacggtcag tgatagctgc tgggtttata cccaatgacc ttgcagcaga tgctcttgag
    27481 gggaatgtaa gtgtttcgcc agttacagta tcctccaata ctgtattttt agataattta
    27541 tctattgtat cttctggaat tttgtgggaa ttgacaccat aagatgcaca ttttggacac
    27601 ccacttttag agtccaacat acttttgaat gtagagcaag taaccctccc gtgtatagga
    27661 cagacgatta ccgtcgggta acgaacgcca ttgaactcca caagattcaa atgtggaaat
    27721 ttagaggtta gcttagaaac tgcttgagta aatgtaagtg ccatagtggt tgtgaactta
    27781 ctttaccaac ctataacgat acctaatcaa tcggtcaaac ggagtgcgtc cgcttaaacg
    27841 tacagctaaa gtgctgttgg agcaaccgat aaatttactg gcagccagtg tacttgcaaa
    27901 ctcgtagatt tcccctgtct ctgtgtcctc caacctaatg gcaacggcgt ttggagaggg
    27961 cttttcggga tttcgagtac attcaggaca tcctgttgag gatttcatgg cagcttgtag
    28021 attcccgtaa acaaccgacc catgcaaagg gcattggatt gttaccggtt cgtagttttt
    28081 agtgaacttc aaaaccgtct tatcaggaaa tttactggaa aaccttaact gaacatcttg
    28141 tagcgagagt gattttggca ttatagacct ttctaaaatg aaagcattat acaaccaaaa
    28201 tatagaaatt ttcaaatttt ctctgaacgc actggtatcc gatggagcta aatgtaaaga
    28261 aaaatggtat acgtctttac taaatcttta cacattttaa ctgtatagtg tttgacaaac
    28321 aacaggcata ggcgcataat gcacccgtag ttagatagat agtaaggaga taaccaatga
    28381 cttagcacct gccccccctc tctctcgcgc accccgcccc tcgcttgcgg ctaatgggct
    28441 ttgcgcgaca tatacacttt catctcctgc atggcttaac tgcctatgca atagtacaca
    28501 catcaacaaa tcaggctggc acgtcttggc attggctgac gtaccattgt agcccgaaat
    28561 gttgataccc tactatttat gaccaacggc ggattagggc ttcagacggc aattttactg
    28621 caaaacctta tgcgaacgga tgatgcagca caatcgtttc ttcgcggtcg gggccggtgg
    28681 agacgatggc gaccggcgca ccgcagactt cttcaatccg tttcaaatat gctttggcat
    28741 tggcaggtaa tgcgtcgtag cttttcacgc cgaaagtgga ttcgctccaa ccgggcatgg
    28801 tttcgtaaat cggcttgcag gcttctaccg catcagagcc gcaaggcagg atgtcggttt
    28861 tgccgccgtc gggcaattcg tagccgacgc agatattgat ggtttcaacg ccgtccatta
    28921 catcgagttt ggtaatacac ataccggaaa tgccgttgat ttggatggag cgtttcaggg
    28981 cggcggcatc aaaccagccg cagcggcgtg cgcgtccggt taccgaaccg aattcgtgtc
    29041 cgcgttctgc caaacctgcg ccgacttcgt cgaacaattc ggtcgggaac gggcccgaac
    29101 cgacgcgcgt ggtataggct ttgacgatgc ccaaaacata atccagcatt tgagggccta
    29161 cgcccgcgcc tgccgaagcc gcgccggcga ggcagttgga cgaagtaacg aaggggtaag
    29221 tgccgtagtc gatgtccaac aacgcacctt gcgcgccttc aaacagcagt ttttcgccgt
    29281 ttttgttttt ttcgttcaac acgcgggaca cgtcggtaat catcggcgta atgcgcggcg
    29341 cgactttttc gataaccgcc atcacgtctt ccgctttcac cgactcggca ttgtgcagat
    29401 gttgcagttg gacgttgtaa taagcaagga cggcatccag tttttcacgc agtttttcag
    29461 gatgcagcaa atcggcggcg cgaatggcgc ggcgtgccac tttgtcttcg taggcagggc
    29521 cgatgccgcg gccggtcgtg ccgattttgc ctttgccgcg cgatgcttct cgggcttggt
    29581 cgagcgcgat gtgataaggc aggatcagcg ggcaggtcgg cgcgattttc agacggcctt
    29641 cgacgttttt cacgcctgcc gcgttcaact cgtcgatttc gcccaacagg gcttcggggg
    29701 agacgacaac gcccgaaccg atgaagcagt ccagactttc atgcaggatg ccgctcggaa
    29761 tcaggcgcaa aatggttttt ttgccgccga caaccaaggt atgacccgca ttgtggccgc
    29821 cttggaagcg caccacgcct ccggcttctt ccgccagcca gtcaacgatt ttacctttac
    29881 cctcgtcgcc ccactgtgcg ccgattacta caacattttt agccatagcc atataacctt
    29941 cgatattaaa aattattgcg gaaatcccca aaccggtgcg gctttgcgcc ccgccacgat
    30001 ttgcctttca tccgccatga acgggtattt agcgtttcac gacctgccaa acgccgtccg
    30061 tctttttcag acggcctgca agctcttccg aaacattgtg tccgataccg taatcgatta
    30121 cgacacactg cccttgttca cgcaaggctt cgaccgcttc gtgcgccgct tcggcatctt
    30181 ccgcatcgac caacacggcg ggctgccgtt cgatggcggg caaacgcccg ataaagctgc
    30241 gcaagtcgaa actgaatccc gttgccgggc gcgccctgcc gaaatatccg cccaatccgt
    30301 cataacgccc gccgcgcgcg accgcgtcgt ggaaatcggc ggcataggcg gcatacagca
    30361 agcccgtgtg gtaattgtcg acgcgcagct cggacaagtc gatatggatt tcacaatcgg
    30421 ggaatgcgtc gcacaccgcc tgcaattcgc ccaacgcgcc gccgaccgcc gacaaatccg
    30481 gcaaccgtcc gcgcgcgtcg gacaacactt cacgcccgcc gtacaggcgc ggcagcagcg
    30541 agaatgcttt tgcccacata ccgtccagct tccaagcctt gacctgcgct tcgaccgtct
    30601 cggtatcttt atcctgcatc aaggcaagca gcgttgcaga ctgccccgca tccaaatgcg
    30661 ccgcatcgga caaggcgcga aatatgccga tatgccccag cgaaagcagc actttgccca
    30721 tatcggcaat tttcatgctt ttcagcatca ggtctatcag ctcgatgtcg ccacggatgt
    30781 cggcaaaacc gtacatttcc gcccctgcct gcaagggttc gcgcatattc agcagaccgt
    30841 cggactgcgc gtgcaacacc ggaccggcat aacacaaccg gttaatccct tggttggcgg
    30901 ataaaagatg ggcatcgata cgcgccacct gcggcgtgat gtcggcgcgt atgcccaact
    30961 gcctgccgct gagcctgtcc gttaccaaaa tggttttcag ggaaagcccc gcatcgatat
    31021 gcgtcagcag ggaatgtgcg tactccatca gcggaggctg taccagttca taaccgtgta
    31081 cgcggaacag tgccaacaac tgctccctcg cgctttcaag ctgccgcgcg ttcgtgggca
    31141 gtacgtcggc gatatgttcc ggaagctgcc atgtctgcat gagaatctgc cttctatttt
    31201 atcctgtaaa cacaaaagac tgcccgatcc gcaatcagac ggcatctttg ccgcaacgca
    31261 tcacgccgct gtcaaaacgg cggtgcttcc ttccgattca gaatccgaac agcccctgtc
    31321 cgacggctca atgccgtcaa gtcgttaaaa ccaaacttta ccataaaata cacacaatct
    31381 gaatatactt ttataccctg tatccgccgt ctgacggcac ggcatttttt tactatataa
    31441 tgtgcgccat tatttctcat cacttcctgc tgccgttttc tgcttcagac ggcatttttg
    31501 ttgattgaac acttatgctg ctcgacctca accgcttttc cttttccgtc ttcctgaaag
    31561 aaatccgcct gctgaccgcc cttgccctgc ccatgctgtt ggcgcaagtc gcacaggtgg
    31621 gcatcggttt tgtcgatact gtgatggcgg gcggtgcggg caaggaagac ttggcggcgg
    31681 tggctttggg cagcagcgcg tttgccacgg tttatattac ctttatgggc attatggcgg
    31741 cgctgaaccc gatgattgcc cagctttacg gcgcaggtaa aaccgacgaa gtgggcgaaa
    31801 cggggcggca ggggatttgg ttcgggctgt ttttgggcgt gttcggcatg gtcttgatgt
    31861 gggcggcgat tacgccgttc cgcaactggc tgaccttgag cgattatgtg gaaggcacaa
    31921 tggcgcagta tatgctgttc accagcttgg cgatgccggc ggcaatggta caccgcgcac
    31981 tgcacgccta cgcctccagc ctgaaccgcc cgcgcctgat tatgttggtc agctttgcgg
    32041 cgtttgtgtt gaacgtgccg ctgaactata ttttcgttta cggcaaattc ggtatgcccg
    32101 ctttgggcgg cgcaggctgc ggactggcga cgatggcggt gttttggttc agcgcgctgg
    32161 cattgtggat ttatatcgcc aaggaaaatt tcttccgccc attcggactg acggcgaaat
    32221 tcggcaaacc ggattgggcg gtgttcaaac agatttggaa aatcggcgca cccatcgggc
    32281 tgtcttattt tttggaagcc agcgcgtttt cgtttatcgt gtttttgatt gcgcctttcg
    32341 gcgaggatta tgtggcggcg cagcaggtcg gcatcagttt gtcggggatt ctctatatga
    32401 ttccgcaaag cgtcggctcg gcggggacgg tgcgcatcgg cttttcgctt gggcggcgcg
    32461 aattttcgcg ggcgcgttat atttcgggcg tgtcgctggt gttaggatgg atgctcgccg
    32521 tgattaccgt gctttccttg gtattattcc gttcgccgct ggtaagtatg tacaacaatg
    32581 atccggcggt tttaagcatc gccgccaccg tcttactgtt cgccggcttg ttccaaccgg
    32641 cagacttcac ccaatgtatc gcctcctacg ccttgcgcgg ctacaaagtt acaaaggtgc
    32701 cgatgttcat ccacgccgcc gccttttggg gctgcggtct gctgccgggc tacctgctcg
    32761 cctaccgttt cgatatgggc atttacggct tctggacggc attgattgcc tcgctcacca
    32821 tcgccgccat cgccttggtg tggtgcttgg aattgtgcag tagggagatg gtcagatcgc
    32881 ataaggctgt ctgaaaactc agtacacttc aattcaaata cagttaaagt tcaaaccatg
    32941 caacccatcc gataccgaac cgacctcacc ccctacaaca ccttcggtct tcgcgcccaa
    33001 gcccgagcct ttattgcgct tgaacacgcc gacgggttgc gcgacatcgt ccggctaccc
    33061 gagttcaacc gagatactgt tttatggctg ggcggcggca gcaatatcct tttgatggag
    33121 gactacgccg gtctggtcgt acatatggaa aacaaaggca tacgcgaaat tgctcgttca
    33181 gacggcatgg ttctgattga agcgcaggcg ggcgaaatct ggcacgattt tgtcctgcac
    33241 gccgtcgcgc tgggtttgaa cggtttggaa aacctgagcc tgattccggg tacggtcggc
    33301 gcgtcgcccg tgcagaacat cggcgcatac ggcgtagagg cgaaagatgt gattcacagc
    33361 gtgcgctgct tcgatttgga tacggaaacc tttgtaaccc tttccaatgc cgactgccgc
    33421 ttcgcctacc gcgaaagcct gttcaagcag gaaggtaaag ggcgttatgt gattgtttcg
    33481 gtcgtatttg cattaaaaac gcattttgtg ccgaacttgg gttacggcga tttggcggcc
    33541 gccgttgccg aactgagcgc gggcagggtg ccgacggcga aagatgtttc cgatgcagtg
    33601 tgtgcaatcc gcaacagtaa acttcctaat cctaacgtgc ttggcaatgt cggcagtttc
    33661 tttaaaaacc ccgtcgtcag cgcagaaaaa gccgccacct tgttgcagcg gcatcctgat
    33721 atgccgcgct atccgcagcc cgacggttcg gtcaaactcg ccgccggctg gctgatcgac
    33781 caatgtcgtc tgaaaggctt tcaaatcggc ggcgcggcgg tacatgacag gcaggctttg
    33841 gtcttagtga acaaaaacaa tgcctcggca aacgatgtcc ggcagttggc gcaacacatc
    33901 aaatttacag tatttgctcg gtttcaggta gaattgcacg ccgaacccaa ttggctgcct
    33961 gcttcgttca gcctgtaaat ccaaggagta tgcccgtgac ccgccccgcc aaaatcaata
    34021 cttacacacg catcatcgac gccagccttg cgctcttcaa cgaggaaggc gagcgcaaca
    34081 tcagcaccaa ccatatcgcg gcgcatttgg gcatcagtcc gggcaatctc tattaccact
    34141 tccgcaacaa agacgaaatc atcgtccagc tcttcaaacg ttacagcgaa gccctgcttg
    34201 cctacctgaa tgaagccgtg ttgccgtctg atgtggaaga ctccatcaat tatatggccg
    34261 gtatttatga tgtgatgtgg gaataccgct tcctattcag cgacgtgaac accctgcttg
    34321 cacgcagtgc cgaactattg ggcgaacaca ataccttcac ccaagccaaa gtctccccgc
    34381 tcttggtcaa cctgctgacc cagctcaacg gtctgaacat catccaagcc gaccaaaccg
    34441 ccatgaacga tctcgccgtc aatatgtgga tggtcacgaa atactggttc gacttcgaca
    34501 gctccctgcg cggccgcacc aagctgaccg aagactccaa agcacgcggc atccgccgca
    34561 ccttaagcct ccttcgtcct tatcttttgc ccgaacaccg cgagaaatac gaccggaaaa
    34621 tcggcaacgg caacccgtaa acccacagcg tccgaatcag ccttcagacg gcacttaccc
    34681 gacggtatca gcgaatgcgc gcagtccccc cgccccatat ctccatcaca ggcctaggtt
    34741 acctcggcct gccgctggca caaaagtttt accaacacag cagccgcgtc gccgccataa
    34801 aacgcagcct gacttcggac gacatcaacc tgcccataca tctcgacacc ttcgacctcg
    34861 gcagcagcga tgcctttcag acggcattgt ggcggcacca cgccgacaaa cccgtctggt
    34921 tcttcctgct accgccctcc tcgctgacgc attacgccga caccgtcaaa caatgggcag
    34981 agcttgcccg ggcgtgcaac gtgcaacacc tgattttcac gagcagcacc agcgtttacg
    35041 gcgataaggc gcgcgaatgc gacgaaaccg ccctacccga tccgcaaacc gagtctgtcc
    35101 gccaaatcct cgccgccgaa caacacctgc tcgacagcgg catctccaac atcgacatcc
    35161 tgcgacttgg tgggctttat tgcgccgaac gccatcccgt cagccgcctt gttcaaaagc
    35221 aaaacatcct gggcggcaac cgccccatca acatcgtcca ccgtaatatc gccgtcgaaa
    35281 gcctgtttca gacggcattt aaccccggcg gcaggcggct gaaaaacatt atcgaaccgc
    35341 gccacccgac acgacgcgaa ttctatacgg aagaagccgc caaactcggc ttgccggctc
    35401 ccgatttcgc acccgacgac agcgtgggca aaatcatccg taccgtttgc gataacggct
    35461 taagcctgta aaataagcgt caacagcaac aaacaggcat tctccgcccc gatcagaaaa
    35521 tatgtccgcc ctcctcccca tcatcaaccg cctgattctg caaagcccgg acagccgctc
    35581 ggaacttgcc gcctttgcag gcaaaacact gaccctgaac attgccgggc tgaaactggc
    35641 gggacgcatc acggaagacg gtttgctctc ggcgggaaac ggctttgcag acaccgaaat
    35701 caccttccgc aacagcgcgg tacagaaaat cctccaaggc ggcgaacccg gggcgggcga
    35761 catcgggctc gaaggcgacc tcatcctcgg catcgcggta ctgtccctgc tcggcagcct
    35821 gcgttcccgc gcatcggacg aattggcacg gattttcggc acgcaggcag acatcggcag
    35881 ccgtgccgcc gacatcggac acggcatcaa acaaatcggc aggaacatcg ccgaacaaat
    35941 cggcggattt tcccgcgaat ccgagtccgc aaacatcggc aacgaagccc ttgccgactg
    36001 cctcgacgaa ataagcagac tgcgcgacgg cgtggtacgc ctcaacgaac gcctcgaccg
    36061 gctcgaacgc gatatttgga tagactaacc ttcagacggc atccgacatg aacagccctt
    36121 ttcacaacat cggcatcgta acccgcccca acacgcccga catccaagac accgcgcaca
    36181 cgctgattac ctttttgaag cagcacggct ttaccgtcta tctcgacgaa gtcggcataa
    36241 aggaaggctg catctatacc caagacaccg tcggctgcca tatcgtcaac aagaccgaac
    36301 tggggcaata ctgcgacctg gtcgccgttt taggcggaga cggcaccttt ctctccgtcg
    36361 cccgcgaaat cgccctgcgc gccgttccga ttatcggcat caaccaaggg catttgggct
    36421 tcctgaccca aattccccgc gaatatatga cggacaagct attgcccgtt ttagaaggga
    36481 aataccttgc cgaagagcgc atcctgattg aggccgcact catccgcgaa ggcaaaaccg
    36541 ccgaacgcgc catcgccctc aacgatgccg tcctctcccg tggcggcgct ggacagatga
    36601 ttgagtttga agtcttcgtc aatcgggaat tcgtctatac ccagcgttcg gacgggctga
    36661 ttgtctccac ccccaccgga tcgaccgcct attcgcttgc cgccggcggc cccatcatgc
    36721 aggcaggatt acacgccttc acactcgtac ccatctgccc gcaatccatg accaaccgcc
    36781 ccatcgccat tcccgacacg tctgaaatcg aaatcctcgt tacccaaggc ggcgacgcgc
    36841 gcgtccattt cgacggtcaa acccatatcg acgtgcaaaa cctcgaccgc atcaccatcc
    36901 gccgctaccg aaatccctta cgcattttgc acccgaccga ctaccaatat ttcaaaaccc
    36961 tgcgccaaaa actgcactgg ggcgaacaat tggtctgaac cgacccttac cggaaaacac
    37021 ctatgaatcc aaaaactccc cccgaccata cagaagatgc cgtgcgcctc tccaaacgca
    37081 tggcgcaact ggggctttgt tcgcgccgcg aagccgacgg ctacatcgaa cagggctggg
    37141 taacggtcaa cggcaaaacc gccgtactcg gtcagaaagt ttcaccggca gaccgtatcg
    37201 aactgaacaa gaaagcccac gaacagcagg cggcacgcat taccatcctg ttgaacaaac
    37261 ccgtcggcta tgtcagcgca caagcggaaa aaggctataa atccgccgcc gaactgatta
    37321 cccctgaaaa tcactgggaa ggcgataccg gccgcatccg tttcgatccg aaacacaaaa
    37381 tcggcctcgc ccccgccggc aggctggaca tcgactcggt cggattgctg gtattgactc
    37441 aggacggccg tatcgccaag cagcttatcg gcgaaaacag cggcagtgaa aaagaatatt
    37501 tggtgcgcgt gcgcggcaaa ttggacgaaa aaggacttgc cttactgaat cacggattga
    37561 gtttggacgg cgagaaactg cgtcccgccc aagtagaatg gcaaaacgaa gaccaactgc
    37621 gtttcgtatt gaaacaaggt aaaaagcggc aaatccgccg tatgtgcgaa ctggtcggac
    37681 tgcgcgtcgt cgggctgaaa cgcatccgca tgggtaaggt caagctcggc aggctgccgc
    37741 ccggcaaatg gcgttatctt gctcccggcg aatcgtttta aataagcatt aaaaaatgcc
    37801 gtctgaacgt ccttacgttt tcagacggca ttgccttatg cccaaataac ggtttcttcc
    37861 aagggcctgc gcgctttcgt ggcgatttcc tgaacgcggt agccgaatgt cgcggcaacg
    37921 gacacgcccc attctgccgc atcgaacaaa ccgaactgcc cggacaatac gcgttccata
    37981 tcggcatagt tgaaaccttc caccgggcag gaatcgatac ctgccatcgc cgcacccgtc
    38041 atcatgttgc ccaacgcgat ataggtctgg cggcaacacc agtcaaacaa ggcgcgcgaa
    38101 tcgtccaaaa tcttgatgtc gtcggcttga aacgcctgat acctcgccaa agattttgcc
    38161 atggcatccg gttcggtaac gccgcgccgt ttgaggcttt ccaacataaa cgggctgtcg
    38221 aagcgggcat ttttctttgc caaaaacacc accaaatgac tggcggtatc caaagcatcc
    38281 gccatacccc aagaaaacgg cttgattgcc tgtcggattt cggggttttg aaccacaaca
    38341 aactgccaag gctccgaacc gaccgaactg ggcgacaaac gcccgagttc taaaataaac
    38401 tgaaaatcct cggcactgat tttacgtgcc gcatcgtaat gccggcatga tttgcggttt
    38461 ttaaatgcgg atagaacctg ctctttgctt aatactgcca tcgcacatcc tttcattact
    38521 tgtattgcaa attgatttca aaccgatacg gcggcagatt cgccaataac gcccaaacgc
    38581 ctttcagacg gcatttggtt catcgcaaac acgcctacgg gtaggcgata taggcataag
    38641 ccgaatggtt gtggatagac tcgaaattct cgctctcgac gacgaaactc ttgatgcgtt
    38701 tgtcggcgat cagcgaagta gcgacatcgc gcaccatatc ttccacgaat ttcgggtttt
    38761 cgtaggcctt ttcggtaacg tatttttcat cggggcgttt gagcaggccg tagagttggc
    38821 agctcgcctg cgcctccaca taatcgatga cttcctcgat accgacttcg gcatcggcag
    38881 tcaggctgac ggtaacgtgc gaacgctggt tgtgcgcgcc gtattgggaa atttctttgg
    38941 aacacgggca aagcgaggtt acgggaatca tgaccttcat actgtggccg tatgcaccgt
    39001 ctttcatttc gcccgtgagg cagacatcat aatccagtaa agactgaata ccggaaaccg
    39061 gggcggtttt cttgcggaaa aatgggaaag aaacgctgat tttaccggcg cgggaatcca
    39121 aaagcgcgac catttcggca gtcagcctat gcaattgtgc gaaatccaag gcttcagaat
    39181 attgctccat caatgcgaca aagcgcgaca tatgcgtacc cttctgctcg gcgggcaggt
    39241 aaaccgtcat ggtcagacgg gcaatggtgg actggatgcc ttctgcagtt tgcagggtaa
    39301 tcggaaagcg caggtctttg ataccgacct gattgatcgg cagattgcgc aaatctcggc
    39361 tggattgcac gtctgcaata gtgttcatga gttgtttttc cttaatattc atgtaattgt
    39421 tgagatgtgt gcatggtatg gcgtttccgc cggggaatat accgtcaatc tgcaaatgcg
    39481 agattatatc actcgctttt agcagctgca ttgattgttt ctatttttca gatagtgata
    39541 aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc cacgaaagcc
    39601 attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa
    39661 aacagtatgt ttgcgttgca cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg
    39721 agcaacccga cccgtcagat tttagaagac accgttgccg atttggaaca cggtgcggca
    39781 ggttttgcgt tttccagcgg tatggcggga attgatgccg tatggcgcac tttcctgcgc
    39841 ccgggcgata ccattgtcgc cgtcgccgat atttacggcg gcgcttatga tttattggtc
    39901 gatgtttatc aaaaatgggg ggtgaacgtt gtttttgccg atttaggcaa tccggataat
    39961 ttggacgaac tgcttaaagc gcacaaggtc aaactggttt ggctggaaac gccgtccaat
    40021 ccacttttac gcttggtaga catcaaagtc cttgccgcaa aagccaaagc agccggtgcg
    40081 ctggtcggta tcgacaacac ttttgccacg ccgtatctgc aacagccgtt ggatatgggt
    40141 tgcgattttg tattccattc cgctaccaaa tatttgtgcg gccattccga cgtgttgatg
    40201 ggcatcgtcg ttgccaaaac caaagaactg gcgcagcctt tgcacgatat gatggtgcat
    40261 accggcgcgg ttgccggccc gctggactgc tggctggtgt tgcgcggcat caaaacactg
    40321 gctctgcgca tgaacgccca ttgccaaaac gcactcgaaa tcgcgcgccg tttggaagcc
    40381 catcctgcca ttgaaaaagt gttccatccc ggcctgccgt ctcacgaaca ttacgaactg
    40441 gcgaaaacac aaatgcccaa aggcatcggc ggcgtggtta cggtttatct caaaaacgac
    40501 acgcgtgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc
    40561 ctcggcggtg tggaaagttt ggtcaaccat tgctattccc agtcccacag cggcgtaccg
    40621 catgatgtga aaatggaaat gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc
    40681 gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacgac tttgtaaact
    40741 gtaaaaatgc cgtctgaaac catggtttca gacggcattt caattaaccc ggccgaaaat
    40801 caacgcttca acatctttgc cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc
    40861 gcacgtttga atgccagcag gctttccaaa accactttgc cgccgtccag ccagccgttg
    40921 gcaatcgctg cctgcaacat cgcgtattct cccgaaacct gataggcata agtcggtacg
    40981 ccgaactcgt ccttcacgcg gcggacaacg tccaaatacg gtaaaccggg cttcaccatc
    41041 accatatccg caccttcccg aatatcgagc gccacttcat tcagcgcctc atcggtattt
    41101 gccggatcca tctggtaggt tttcttgtcc gccttgccca aattgcccga actgcccacc
    41161 gcatcgcgga acggcccgta aaacgccgaa gcatatttgg cagaatacgc cataatccgc
    41221 gtatggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc
    41281 atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc
    41341 aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag cccgtcctga
    41401 ccgtgaaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc gggaaacctc
    41461 tcgcgcaagg cgcggacagt tgacggcacg agtccttcgg gattgtacgc ctcctgcgca
    41521 cgctcggttt tgtttgccgt aaccacgggg aacagtgcca acatcggaat accgagcttt
    41581 accgcctctt ccgctgtaaa cagcagcctg tccaaacttt gacgcttcac acccggcata
    41641 gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata aatcaaatca
    41701 tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg
    41761 cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc aatcatcctg
    41821 tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg gcggcaatca
    41881 aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga aggacggcgt
    41941 cccgcccatt aaagaacaat agcaaaacaa cagcttcagg ctgtcagcct tcgatattct
    42001 tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca accgcctcac
    42061 ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg cgcactttaa
    42121 tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct
    42181 gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt
    42241 ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa gaataatgcg
    42301 gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc ggtttccatt
    42361 ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac
    42421 acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat
    42481 acaaactttt ggaaatcaac gaaaaagaca aattaatcaa acccggcacg gtacttgccg
    42541 acttgggcag cgcaccggga agctggtcgc aggttgccgc caagctgacg ggtacttccg
    42601 gagcagtttt cgccttggac atcctgccta tggaagccat agggggcgtc tccttcattc
    42661 agggcgactt ccgcgagaac gacgtactgg cacaattcga aaccttgttg gacaaccgcc
    42721 cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc
    42781 aggcacgcag cttttatctg tgcgaactgg ctttagactt cgcctcgcaa cacctgaaaa
    42841 ccggcggcag ctttttggtc aaagtctttc agggtgcagg ctatcaggaa tacatggcag
    42901 ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctcg cgcaatcgct
    42961 ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg cgtgctttac
    43021 aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac acctttaaat
    43081 caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac gctttagacg
    43141 gtaaaaaaga agacaacggg caaatcgaat attctcagtt catccaacag gtcaacaacg
    43201 gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg attaagggcg
    43261 agcgcaccga caaaagcacc ttcttcacca acgcgccttt ggacgacaac ctgattaaaa
    43321 cactgctcga caaaaacgtc cgtgtaaaag taacgccgga agaaaaaccg agcgcgctgg
    43381 ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcgtgg ttctacttta
    43441 tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggc aaaagccgcg
    43501 cccgcctact ggacaaagat gccaacaaag ttacctttgc cgatgtcgcc ggctgcgacg
    43561 aagccaaaga agaagtgcag gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa
    43621 gcctgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt acgggtaaga
    43681 cgcttttggc gaaagcgatt gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt
    43741 ccgactttgt cgaaatgttc gtcggtgtcg gtgcaagccg cgtccgcgat atgttcgagc
    43801 aggcgaagaa aaacgccccc tgcatcatct ttatcgacga gattgacgca gtcggccgcc
    43861 aacgcggcgc aggtttgggc ggcggtaatg atgagcgcga gcaaacatta aaccaattgt
    43921 tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg gcaaccaacc
    43981 gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc caagtggttg
    44041 tccccctgcc ggacatccgg gggcgcgaac agattttgaa cgtccactct aaaaaagtgc
    44101 ctttggacaa atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg
    44161 cggatttggc gaacttggtc aacgaagccg ccctgtttgc cggccgccgc aataaagtca
    44221 aagtcgatca gagcgatttt gaagacgcca aagacaaaat ctatatgggt ccggaacgcc
    44281 gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg
    44341 cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc attatgccgc
    44401 gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc agtatgtata
    44461 aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc gaagacatct
    44521 tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc caaatggcgc
    44581 gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa
    44641 acgaaggcga agtcttcttg ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa
    44701 cccagcagga catcgatgcg gaaatccgcc ggattttgga cgagcagtat caggttgcct
    44761 acaaaatcct cgatgaaaac cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat
    44821 gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa cccagcccgc
    44881 ccaaagatta cagccacaac ctgcgcgaga atgcggacgc ggcggaagat aacgcgccgc
    44941 acgctccgac tcgggaagaa accgaagcac ctgccccggc agacaccgct tcgacagagt
    45001 ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc
    45061 cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga aacgccaaag
    45121 ccgtatcggt ttttgcgaat ctgcgatatg gcggtttaaa ttgaaatcag aacggggcaa
    45181 ggctgtaccg gtttaaaatt aaaccgctat aataccgccc ttcgaataca cgcccgcatt
    45241 ggtgatgccc gtgaaaaaag ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat
    45301 gttcgagctg gtcgataagg ttgaggatta cccgaacttt ctgccgtggt acagcaaaac
    45361 cgaggtcatc ggacgcaacg gcaacgaact gaaggcgcgg ctgtttatgg actatatgca
    45421 cgttcgccaa tcattcgcca cgcacaaccg caacattccg ggcagggaaa tccgtatgga
    45481 actgctcgaa ggtccgttca aaaccttacg tggaacgtgg aaatttatcg atttgggcga
    45541 cgatatgtgc aaaatcgaat tcaatttgga atacgatttt tccaatgccg ttttgtccgc
    45601 cttaatttcc cccgtcttca accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga
    45661 ggcagaccgc cgttatgctt gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt
    45721 tgaaaaccat gcagcttgcc gagggaacaa ccgtccgcgc cgccgcactg caaagcggtt
    45781 tggacggcat atttgaagat ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag
    45841 ccgtcaaaga cgacacgccg ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt
    45901 tgatcgaccc caaagaagcg cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc
    45961 gtctgaagcc ttcagacggc attcggcaga aacacggaaa atatcatgtc aaacacaatc
    46021 aaaatggttg tcggcttggg caacccgggc aaagaatacg aacagacccg ccataatgca
    46081 ggcttttggt tcctcgacga actggcttgg aagtggaagg cttcgtttaa agaagaaaaa
    46141 aaattcttcg gcgaagtcgc ccgtgctacc ctgcccgacg gcgatgtctg gctgctcaag
    46201 ccgaccacgt tcatgaaccg ttccggacag gcagttgccg cccttgcgca gttttataaa
    46261 atcaaacccg aagaaatcct cgtcgtccac gacgaactcg acattccctg cggacggatc
    46321 aaattcaaac tcggcggcgg caacggtgga cacaacggct tgaaagacat tcaggcacgg
    46381 ctcggcacgg ccgactatta ccgcctgcgc ctcggtatcg accacccggg cgaccgcaac
    46441 ctcgtcgtcg gctatgtcct gaacaaaccc agtacggaac accgccgaca gattgacgat
    46501 gccgtcgcca aatccctgca ggccataccc gacatccttg ccggcaaatg ggaagaggca
    46561 acccgcttcc tgcacagcaa atgacccgat gccgtctgaa gccctttcag acggcatgtt
    46621 cccgatttcc atatccgaac agtcatgacc gaactcaagc agcttatcca aaccgaatcc
    46681 atcctcgtca tcgaagaaac cctcgatttc ctgctgtacg aatgcagcat cgacgacgca
    46741 ccatccgccg aagaagtggc acaatggcgc gacatacttg ccgcacgcgg cggcaaattc
    46801 ctgcgcctgt ccaaaatctg ccaaacgtgg ctggacgagg aggcggcatg aagctgccgc
    46861 gcaaccgctt cagcctgctt tccgcattgt ggtttgccgg cggcatctat tcgctgctct
    46921 tcaaagctgc cgacaccgcg ccgccgccgt ttccgcattt cgacaaagca gcacaccttg
    46981 ccctgttttt cgcacaaatc tggcttttga ccaaagcatt caaaaccgga aaacttccca
    47041 tcccctaccg cagcctgatg gtctttgccc tctgtttcgc cctcttcagc gaatgcgcgc
    47101 aggcatggtt taccgcaacg agaaccggca gtttgggcga tgttcttgcc gatatggcag
    47161 gtacggttct cgcactcttt gccgcccgcg ccgccgaccg cccggactga atcggtttat
    47221 ttcccccaaa caggatgcac ctcttcccga tacatcctat ttttcccctt acatatcaat
    47281 cccatctcat aaacaaaacc gacaaatcgt ttacaatata tttacactac atcagattac
    47341 aaatatactc gaaccagttg cagaatcggc ggttccacaa accgtttcgg attgcacgac
    47401 acaaaagaaa aaccgatttt attgccttaa aggagtattc atgaacctgc atgcaaagga
    47461 caaaacccag catcccgaaa acgtcgagct gctcagtgcg cagaagccga ttaccgactt
    47521 taagggcctg ctgaccacca ttatttccgc cgtcgtctgt ttcggcattt accacatcct
    47581 gccttacagc cccgatgcca ataaaggtat cgcgttgctg attttcgttg ccgcactttg
    47641 gtttaccgaa gccgtccaca ttaccgttac cgcgctgatg atccctatcc tcgccgtcgt
    47701 actcggtttc cccgacatgg acatcaaaaa ggcgatggct gatttttcca acccgattat
    47761 ctacattttt ttcggcggct tcgcgcttgc caccgccctg catatgcagc ggctggaccg
    47821 taaaatcgcc gtcgccctgc tgcggctgtc gcgcggcagt atgaaggccg ccgtgctgat
    47881 gttgttcctc gttaccgcct ttctgtccat gtggatcagc aacaccgcca ccgccgcgat
    47941 gatgctgcct ctggcaatgg gtatgctgag ccacctcgac cgggaaaaag aacgcaaaac
    48001 ctacgtcttc gtcctgctcg gcatcgccta ttgcgccagc atcggcggct tgggcacgct
    48061 cgtcggctcg ccgcccaacc tgattgccgc caaagcccta aatctggact tcgtcggctg
    48121 gatgaagctc ggcctgccga tgatgctgtt gattctgccc ttgatgctgc tctccctgta
    48181 cgtcatcctc aaacctaatt tgaacgaacg cgtggaaatc aaagccgaat ccatcccttg
    48241 gacgctgcac cgcgtgatcg cgctgttgat tttccttgcc acagccgccg cgtggatatt
    48301 cagctccaaa atcaaaaccg ccttcggcat ttccaatccc gacaccgtta tcgccctgag
    48361 tgccgccgtc gccgtcgtcg tcttcggcgt ggcgcaatgg aaggaagtcg cccgcaatac
    48421 cgactggggc gtgttgatgc tcttcggcgg cggcatcagc ctgagcacgc tgttgaaaac
    48481 atccggcgcg tccgaagcct tgggacagca ggttgccgcc accttttccg gcgcgcccgc
    48541 atttttggtg atactcatcg tcgccgcctt cattattttt ctgaccgagt tcaccagcaa
    48601 caccgcctcc gccgcattgc ttgtaccgat tttctccggc atcgctatgc agatggggct
    48661 gcccgaacaa gtcttggtat tcgtcatcgg catcggcgca tcttgtgcct tcatgctgcc
    48721 ggttgccaca ccgcctaacg cgattgtgtt cggcacgggc ttaatcaagc aacgcgaaat
    48781 gatgaatgtc ggcatactgc tgaacatcct ctgcgtagta ttggttgctc tgtgggctta
    48841 tgctgtactg atgtaaacca tcgacctaaa caacaagacc gtctgaaaga atatttttca
    48901 gacgaccttg aagtttgtcg ttcagacaca atttgtcgaa tcattcaaaa ccggattcta
    48961 acgaaaggaa acccatgatt atcctgcaca ccaacaaagg cgacatcaaa atcgaactcg
    49021 atttcgacaa agcccccgtt accgccaaaa acttcgagca atacgtcaaa gacggcttct
    49081 acgacggcgt aatcttccac cgcgtcatca aaggcttcat gattcaaggc ggcggcatgg
    49141 atgaaaacat gaacgaaaaa gaaacccgcg atccgattca aaacgaagcg tccaacggcc
    49201 tgcccaacga taaatacacc atcgccatgg cacgtacttc cgacccccat tccgctggcg
    49261 cgcaattctt catcaacacc gccgacaatg ccttcctcaa ccaccgctcc aaagaaatgt
    49321 acggcagaac cgtcgtccaa gactggggtt atgccgtatt cggcaaggta gtcggcggtt
    49381 ttgacgtggt cgatgccatc gaaggcgtat ccaccaaacg ccacggctac catgatgatg
    49441 tgccgacaga acctgtcatc atcactaaag ccgaagcggt ataaaccggc aatccgaaag
    49501 cagcctgcac aaaggctgct ttttttgttt caggcggcat aaaatccaag cacatatgcc
    49561 gtctgaacac ctcccgcaag ccttcggaca atgttgcaac gtgttaaaat agcctccttt
    49621 cctgaaacca caacaaggaa accatcatgg caaatattgc ccgcgacatc ttcaaagcct
    49681 acgacatccg aggcattgtc ggcaaaaccc tgactgacga tgccgcctat ctcatcggca
    49741 gggccatcgc cgccaaagcc gccgaaaaag gcatgacccg catcgcgctc ggacgcgacg
    49801 gacgcttgag cggtcccgaa ctgatggagc acatccaacg cggcctgacc gacagcggta
    49861 tcggcgtcct caatgtcggc atggttgcca cgcccatgct ctattttgcc gccatcaacg
    49921 aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcctgat tacaacggtt
    49981 tcaaaatgat gctcggcggc gacacgcttg ccggcgaagc catccaagag cttttgtcca
    50041 tcattgaaaa agacggtttt gctgccgccg gcaaacaagg cagcgtaacc gaaaaagaca
    50101 tctccggcga ataccacaac cacatcgtcg gacacgtcaa actcaaacgc ccgatgaaca
    50161 tcgctatcga cgcgggcaac ggcgtgggcg gcgcatttgc aggcaaactc taccgcgctt
    50221 tgaggtgcgg cgttaccgaa cttttctgcg acgtggacgg caatttcccc aaccaccatc
    50281 ccgacccatc caaacccaaa aacctgcaag atttgattgc cgcgctgaaa aacggcgatg
    50341 ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt accaaagacg
    50401 gcaacattat ttatcccgac cgccaactga tgctgttcgc gcaagacgtg ttgagccgca
    50461 accccggcgc gaaagtcatc ttcgacgtga agtccacccg cctgcttgcc ccgtggatta
    50521 aagaacacgg cggcgaagcc ataatggaaa aaaccggcca cagcttcatc aaatcgacca
    50581 tgaaaaaaac cggcgcactc gtcgccggcg aaatgagcgg acacatcttc ttcaaagaac
    50641 gctggttcgg attcgacgac ggtctgtacg ccggcgcacg cctcttggaa atcctgtctg
    50701 cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc acgcccgaac
    50761 tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa ctcgccgcca
    50821 aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtcggcgc gttgaatttc
    50881 ccgacggctt cggtctgatg cgtacttcca ataccacgcc gattctggtg ttgcgttttg
    50941 aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc gtcatcgaaa
    51001 gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc aaatcttggt
    51061 agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt cgaacaactc
    51121 tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg tcgtggcaac
    51181 ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt cccgcgccaa
    51241 ttccttcatg gcgttcaaca cgtcttgcac caactcgggg tcaagcgcgg aagtgggttc
    51301 gtcaaacaac atcagctcgg gctgaatcgc caatgctcgg gcaatgccga cgcgctgctg
    51361 ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc cgactttttc
    51421 cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta cggcaaccgg
    51481 tccttccatc acgttttcca aggcggtttt gtgcggaaag aggttgtatt gttgaaacac
    51541 catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt tagaaaaatc
    51601 gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca tttccaacgc
    51661 gtttaggcat cgcagaaacg tcgttttgcc tgagccggaa ggcccgagga tgacgaccac
    51721 ctgccctttg cacacatcca aattgatgcc gcgcaaaatg gtatttttgc cgaaggtctt
    51781 atggatattg cggattttaa tcatgacaac tccttatttg gcgacgtagc ggtcgaaacg
    51841 tttttccaaa cgcgcctgaa tcaggaacag cactttacaa aaacaccagt aaaccaaagc
    51901 ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct gcgcgacgcg
    51961 gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca aaccgataaa
    52021 ctcgttgctc aaaggcggca cggcaacgcg gaatgcctgc ggcgcaacga tgcggcggaa
    52081 cgtctgcata taggtcatgc cgatggagaa acctgcttcc cattggcctt tcggtacgga
    52141 caaaattgcc gcgcgtatgg tttcggaagc atatgcgccg acattgagcg aaaagccgat
    52201 gatggcggca ggaatcgggt cgatatagat gccgacggaa ggcagcccgt aaaacacaat
    52261 cacaagctga accaacagcg gcgtaccgcg aatgacggaa atataaaatt ccaccaattt
    52321 cagcaggatt ttccgcacga tgccgccggc gggcataatc cgcaccaaag ccacggctac
    52381 cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa ggcagagaga ccgcgaagcc
    52441 ggctttgacc ataggcaaaa acgcgctgac aatcatatcg gcgcgtgttt ccgtcataaa
    52501 cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt tccgaagaat tgttcgccca
    52561 gttttttcag cgtaccgtcg gctttcagct cgttgattgc cgtactgaat ttcgccacgg
    52621 cttcgtcatt gcccttgttg acaatcaggc cggaaccgac tttttcatcg gcaggtgcgg
    52681 accaaacgat tttcacgccc gcattcgggt ttttcttcag atagtccaaa accgccaatt
    52741 cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag ggtcagcgat tgcgccaaac
    52801 cgtccactgc caccaaatct gcgcctgcag ctttggcttt ttcgccgtag ttgctggtca
    52861 gggattgtgc ggttttcacg cctttgatgt cgtcgataga tttgatgttg ctgtcgttac
    52921 gggcaaccaa tacggcaccg ctccagctgt aaggatcgga tttgtcgaat gtcgcttggc
    52981 gttcggggct ggtcagaccg acttggtttg ccaccacgtc gaaacgcccc gccttcaaac
    53041 ccgccatcat cgaatcccat tgcgtttctt taaactcgac tttcacgccc agtttttccg
    53101 ccacggcgcg ggttacttcc acatcgtaac cggtcagttt gccgtccttg tcgtggtagg
    53161 taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt gcctttattg ttgatgcgct
    53221 cgattaaaga accggaaact gccgattgtg caggcgcgga agatgctccg ctgccgcttt
    53281 ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc gccgagtacg aattttttca
    53341 acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt ctaagtgaaa ttgcggcaaa
    53401 cacaaagaat acttttttta tatggttatt ccaaatcgga atggaaaaga tatgccgaag
    53461 ccgccggaag cggaaataaa gggggcggga aaggcggcgg gcggcacgaa accgaaaagc
    53521 ggcatcaggt atgcaaacgg cggattcacg cacgccgttc cggcaaaaat ccgcctgccg
    53581 attcggtgct ataatccatc cgaaacagat gacaccaaaa aggaacatct catgaacacg
    53641 ccccaatccg ccatcattcc cgaccacgcc caagccggca tctttatcga agccgacttc
    53701 gccgccaacc gcctcaacga tattaaagcc gcctgccgcg cttcgctcga cgcgttatcc
    53761 gccttgaaaa cccgctttcc ggacgatatt ttgggtctga ccatcgcctt cggcagcaaa
    53821 gcctgggcaa cattcggaca tacggacgaa ggcagtgaaa tcaaaccctt ctccgaaatg
    53881 ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca tccacatcca atctttccgc
    53941 caaaacgccg cctacgcgct tgcccaatcc gttttgggcg cgttcggcga cagcatatgc
    54001 gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc gcgggctgga cggtttcgtc
    54061 gacggcacgg aaaacccgca gggcgatgaa aatgtccgcg aagtcgccat catccccgaa
    54121 ggaaaacccg atgcgggcgg cagctatgtc ctgctgcaaa aatacctgca cgatttgaaa
    54181 aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg tcggacgcgg caaggaaacc
    54241 gacgacgaat tcggccacga tgtccgcctg cccgattcgc acctcggccg cgtcaacctg
    54301 aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc tgcccttcgg caaaatcagc
    54361 ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc tgcacaatat cgaagcgcaa
    54421 ttattgagta tgttcggcga cacggatggc aaaaccgacc tgctgctccg gcacctctcc
    54481 gccgccgttt cgggcggata ctactacgcg ccgtctgtcg aacgcctgca aaacctctaa
    54541 gccagacgat gccgtctgaa accatttttc agacggcatt ttcccgtttt atagtggatt
    54601 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
    54661 tgaagcacca agtgaatcgg ttccgtacta tttgcactgt ctgcggcttc gtcgccttgt
    54721 cctgattttt gttaatccac tataccgtcc aaccgttttc cgagcgcgca ctatgtctgc
    54781 aagcatccaa gcattcgacc cgctaaccgt ccccatttcc ggcaccaacc tgattgaggc
    54841 ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacacgcc tgatcgtatt
    54901 agaacaaaaa aacgtcgaac gcgtattggt cgtaaccttc accaaagccg ccaccgccga
    54961 gctgaaaaca cgcctgcgcg ggcgtttgga cgatgtgtta caagttttag aaagcaaagg
    55021 aattgccaaa cttggagacg acacgctttc agacggcatt gccgcctact gcgccgaaca
    55081 ccacgaaggc gacaccttcc tgcccgaact cttaaaacag gctttgcaaa aagagggccg
    55141 gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg ccgccatcta
    55201 taccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcctgt gccaagcacc
    55261 gttcgatgtc gaaatgaccg aagaagacgg cgaccgcctg cttatcccgg cgcaagattt
    55321 ttggcgggaa cgcgtcagca atgatccggt gcttgccgca ttggcgctta aacgcaaagc
    55381 tgtgccgcaa accgtccttg cccaaatttc gcgctatctt tcccgcccgt acctgaattt
    55441 ccgccgtccg caggcggatt tgaaacaggc gcagcgcaac gccgaaacct catggcaaac
    55501 catctgccgc ctgctgccag aactggaagc cggcttttgg cgcattcatc ccgacctcaa
    55561 cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg cacaaaaatc
    55621 cgccgccgga caactgccct ttctggacaa agacacacac gacagactct tgaaacttgc
    55681 atccgacaaa ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg cagtatttgc
    55741 cgaattgcag aaactggcag acttcgggcg cgatttgaac gcactcgaag aagcggaaga
    55801 agcaacaatg atccggctgc aactggattt aatcgaatac ctcaaccgca gccttgccga
    55861 gatgaaaaaa tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg tccacaccgc
    55921 gctgaccgac aatccgcacg ccgacactct cgcccgcgcc gttgccgaaa actgggaaac
    55981 cgcgctgatc gacgagttcc aagacaccga cccgctgcaa tacgaaatct tccaaaagat
    56041 tttcatcgcc caaaaccgac cgctgttcct cgtcggcgat cccaaacagg cgatttacag
    56101 ctttcgcgga gcggacattt acgcctacct tcaggcggcg gaagacgcgc ggcaccgcta
    56161 cacgctcgcc accaactacc gcagccacgc cgcgcttatc ggcagcatag gcgcgctgtt
    56221 ccgcctcaaa gaacgcccgt tcgttttgga aaacatcggc tattcggaag tcggtgcggc
    56281 gcgtgccgaa agcaggctgt cccccgaacg tcctgccgta caggttcgtt ggctgcacga
    56341 aaacgacaat gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg actattgcgc
    56401 cgacgaaatc gcccacgcgc tcaacgaagc cgccagaggc cgtctgaatt tcaaaggctg
    56461 cccgttgcag tcgggcgata ttgccgtgct ggtacgcacg cacaacgagg cggtgatggt
    56521 ttccgccgcc ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg aatccgtgtt
    56581 cgcctcgccc gaagccgccg ccctgtccgc actcatcggc ttctggctcg aaccgcgccg
    56641 cgccggaacg ctgcgttttg tcctgacaag cagcatattc ggttatgacg cgcagcaatt
    56701 gcacgacttc aaccaaaacg aaagcgagat tttgcattgg gcggaatctg cccgaaccgc
    56761 actcgacaac tggaataaat acggcatttt cgccgctatg cagcaatttt cccaaacaca
    56821 cggcatcgaa acgcgcctct taagccggaa caacgggcgc agtctgacca attatttcca
    56881 actgctcgaa ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg cgctgcacaa
    56941 atggctgggc gaccaaatca gccttgccga aaacaacagc ggcgacaacc gcgccatccg
    57001 tctggaaagc gacgaagatt tggtcaaaat cgttaccatg cacgcctcga aaggtttgca
    57061 gtatccgctg gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac cgtccgactg
    57121 gcaaatcctc caccaaggcg caaaccgaac cgaactgttg gcaaaggcgc aactgtcgga
    57181 agacgaacag aaacaatacg ccgatgaaga aatggcggaa cgcctgcgcc tgctttatgt
    57241 cgcgctgacg cgtgccgagg aacagctcaa catctacgcc gcgtactcca ccaatactgc
    57301 cgacaacccc ctcgcctacc tgattgaagg ctcgccccaa gacagccgtg aaaccgtccg
    57361 ccgtgcctat gcgtgtgaaa aagacggcat cgcgatgctc aaacgcaact ggcggcgcgt
    57421 ggcggacaac gccccttccg gcacaaattt cgccttcacc gaaaatgcgc cgccccctgc
    57481 cgcctatcgc agcaacgccg gtcaaaccgc cgaatttgcc gcaaacagca ttcccgaacg
    57541 cggattccga tttgtccgcc acactagctt taccgcctta agccgccata cccaaacgcc
    57601 cgacggcggc gaagaagatg cctgcccgtc cttggatgcc gccgaaacct cggtgccggc
    57661 gatgccgtct gaaacgccga cggcttcaga cggcatatcg atacacgatt ttccgaaagg
    57721 cacgcaggcg gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg gacaagcagc
    57781 cgccggacag gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg aagaaatatg
    57841 gctgcccgcc gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga cgggggcata
    57901 cgacctgtcc gacatctcac ccgagtgccg ctgtcccgaa atgggcttta ccctccacac
    57961 cgaagacttc agcctcaaac gcctgcgcga ctggtttgcc cgcgacgaca tcaggctgcc
    58021 cgaagtctgc cgtgccgctg ccgaaacgct cgacttccac accgtcaacg gctttttaaa
    58081 cggctttgtc gatatggtct gccaagaccc cgacggcaat atctgcgtca tcgactacaa
    58141 atcaaaccac ctcggtacgg acgcatccgc ctacacgcaa caggcaatgg acgaagccgt
    58201 cgcacaccac cactattacc ttcaggcact gatttacgcc gttgccgccg cacgctactt
    58261 caagctgcgc ggacaaccgc ccgccgccgt ttccgtccgc tacctgtttt tgcgcggatt
    58321 ggatggcaaa ggcggcggcg tttggcgttg ggacatagat gccgccgctt tggaacagat
    58381 aaaataactt cccgtccttt cggacaacaa ccgcaggaaa accctatgaa tatcaaacac
    58441 ctgattaccg ccgcactcat tgcctcagcc gcctttgccg cgcaggcagc cccgcaaaaa
    58501 cccgtatccg ccgcccaaac cgcgcaacat ccggccgttt ggatcgatgt ccgttccgaa
    58561 caggaattta gcgaagggca tttgcacaac gcggtcaaca tccccgtcga ccaaatcgtc
    58621 cgccgcatac acgaagccgc gcccgacaaa gacacgccgg tcaacctcta ctgccgcagc
    58681 ggacggcgtg ccaaagccgc ccttcaggag ctgaaaaaag caggttatac aaatgttgcc
    58741 aatcacggcg gttatgaaga cctgctcaaa aaagggatga aatgacgcaa tgccgtctga
    58801 aaggacaggc tttcagacgg catacggcgt tccggcacgc ggcgcaacat cgggcgcggc
    58861 gcgcgccgcc ccgtccgaag cctgataaac aaaccgataa ggaacaatga tgtttcacga
    58921 cacataaagc accgccctat gtgttgccct gatttggaaa gggttacacc cctcccgaat
    58981 aaagtctgat tctaccgttt caaccaaaaa ggaaaaagga aatacgatga aacacatact
    59041 ccccctgatt gccgcatccg cactctgcat ttcaaccgct tcggcacatc ctgccagcga
    59101 accgtccacc caaaacgaaa ccgctatgac cacgcatacc ctcacctcaa aatacagttt
    59161 tgacgaaacc gtcagtcgcc ttgaaaccgc cataaaaagc aaagggatgg acatttttgc
    59221 cgtcatcgac catcaggaag ccgcccgccg aaacggctta acgatgcagc cggcaaaagt
    59281 catcgtcttc ggcacgccca aagccggcac gccgctgatg gtcaaagacc ccgccttcgc
    59341 cctgcaactg cccctacgcg tcctcgttac cgaaacggac ggcaaagtac gcgccgccta
    59401 taccgatacg cgcgccctca tcgccggcag ccgcatcggt ttcgacgaag tggcaaacac
    59461 tttggcaaac gccgaaaaac tgatacaaaa aaccgtaggc gaataagccc gtcgccgaca
    59521 tatggcggat ggaaatcaaa tgcacacgcc cctcattccc acgaagtcgg gaatccaatc
    59581 ggttgggtta ccgtgatttt caatcattgc cgaaacggac gattccggat tcccaccctt
    59641 gcggggatga cgggcgtgtg tgtttttgcc acatcacagg caactttgcc gcaaatctta
    59701 tctgtcgccg gtatgccttc ttgtttcaat ccgccacaaa ccaaatgccg tctgaaaccc
    59761 gatttcagac ggcatttccg tttattccct gctgtcgcgg caaatatcga tggcttcctg
    59821 caaactcgaa acgccgtaga ttttcaggtt cggaaactct ttggcgttgc gcggcatatt
    59881 ggctttgggg acgatggcgc gtttgaagcc gagtttttcc gcttctttga gccgctcttg
    59941 cccgcgtgcg acggggcgga cttcgccgct taagccgatt tcgccgaaaa ccacggtttt
    60001 ttcaggcata gggcggttgc ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc
    60061 cgccggttcg ccgattttca cgccgccgac ggcgttgagg aacacatcct gatcgaaaca
    60121 ggcgatgccg ccgtggcggt ttaacacggc aagcagcatc gcaagacggt tttgttccag
    60181 tccgacggtg aggcgtttgg gcgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc
    60241 gaccaaaagc gggcggctgc cttcctgtgt aaccaaaacg cacgagccgg gcgtatcgtc
    60301 gcggtagctg gcgaggaaga tggcggacgg gttggacaca cctttcaaac cgttttccgt
    60361 catcgcgaac acgcccagtt cgtttgccgc gccgaagcgg tttttgatgg cgcgtatcat
    60421 gcggtagttg gaatgttggt cgccctcgaa atacagcacg gtatcaacca tgtgttccag
    60481 cacgcgcggg ccggcaatcg cgccgtcttt ggtcacgtgt ccgaccagta tcatggcgat
    60541 gcccatctgt ttcgccatgc gcgtcagttg ggcggcacac tcgcgcacct gcgacacgga
    60601 gccgggggcg gacgtgattt ggtcggaata catggtttgg atagagtcga tgacgacaac
    60661 ttcgggctga tgctgtttca aggccgcctg aatcgcttcc atgcggattt cggcaagaag
    60721 gtttacgccg tcggtcggca gttccaaacg ctgcgcgcgc agggcgactt gttgggcgga
    60781 ttcttcgccg gaaacgtata gcactttacg gctttgcgcc attttggcga tggtttgcaa
    60841 cagcagcgtg gatttgccga tgccggggtc gccgccgagc aggatgaccg caccatcgac
    60901 caaaccgccg cccaatacgc ggtcgagttc gcccataccg gtcggattgc gcggcacttc
    60961 ggtggcggta acggcggaga gggattggac ggtcgaagtg tccgccgccc aagattggaa
    61021 acgggcgttt ttcggctcgg gcgcggcaag gctttcctga agcgtgttcc actcgccgca
    61081 atgcgggcat ttgccttgcc atttcgggga ggtgccgccg cattcggtgc attggtaaag
    61141 ggttttaagc gtttttgcca tcggttttcc ctgcatctga aaacaaaaat gccgtctgaa
    61201 aatagggacg gttgggattg cgccaatacg acaacgccgc cgccgtcatg tactatccat
    61261 acacggcgga tattgccgca ttgccttatt tttaccctaa ccggccatct ctcggcttca
    61321 gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg agacagctcg
    61381 tgtacggtat cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt gatgccgtgt
    61441 ccgagattga agacgtggcc gctgccgtgt ccgtagtcgg ctaggatgcg cgcgacttcg
    61501 gtgcggatgg attcgggtgt gccaaagaga gcaaacgggt cgaagttgcc ttgcagggcg
    61561 acttgcttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc caagcccaat
    61621 gcgtctgcgc cgatttctgc catgctttcc agccacagcc cgccgccttt ggcaaacacg
    61681 atgacaggca cgcgcctgcc ttcgctttcg cgtttcagtc cggcaacaat ttggcggatg
    61741 tatttgaggc tgaattcttt aaacgccgca tcgctcaaga cgccgcccca agtgtcgaaa
    61801 atctgcacag cctgcgctcc cgcgtcgatt tgggcgttga ggtaggcggt aacggcttgg
    61861 gcgttggtat cgaggatttt gtgcagcaaa tcggggcgcg agtacatcat ggttttgatg
    61921 gtgcggaatt ctttgctgct gccgccttcg accatgtaac aggcgagcgt gaacggactg
    61981 ccggagaagc cgataagcgg tacgcggccg tccaatgctt tacggatgga agttaccgcg
    62041 tcgaaaacgt attgcagttt ttccatatcg gggacgtgca atttggcaat gtcgtcctcg
    62101 tgttgcaggg cgcgtttgaa tttcgggcct tcgccttcgg caaaatacag tcccaagccc
    62161 attgcgtcgg ggacggtcag gatgtcggaa aacaaaatcg ccgcgtccaa atcgaaacgt
    62221 tccaaaggtt ggatggtaac ttcggtcgcc aattcggtgt ttttgcacaa atcgaggaag
    62281 ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc ctgacgcatc
    62341 atccaaatcg gcgtgtattc gacgggctgt ttaagcaggg cgcggaggaa ggtatcgttt
    62401 tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg gttttcagac
    62461 ggcatttcaa cgatgccgtc tgaaggtttc aatgggtttc ggcggaaggt cggttttcct
    62521 cggcaacgct tgccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccggttt
    62581 cgtcaaaaac ctttgccaga accaaacgcg cggaaatact cggctttaat gcaatgctcg
    62641 cttcaaggta gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat
    62701 acatcagcag aagcgcgtta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg
    62761 ctttctgctg ttcgcgttcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagct
    62821 cggggcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc
    62881 ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg
    62941 gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc
    63001 ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc gccttggaaa
    63061 gtttttcggt ttttgccaga acctgcaacg cgtcgcccct gtcgaaagcg taacgaagtt
    63121 gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcatga agattggctt
    63181 ccgccgcttc gtaatcgcgc cggttcaacg ccgattccgc caacaaaaga taacgggaaa
    63241 gctgctgctt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc agctcgatgt
    63301 tttccatctg cccggcggca tgtgcgccca acatcaatgc caaagtccgg ttgtctccgg
    63361 cctctttgtt gaccaacacg cgtgaggctt ctagttccgc cttttcaaaa cgcccttcaa
    63421 aatacgccaa acccgccttg ttcaaggcaa gcgcggcctt gcggccttta cgcgccgaac
    63481 cgaaacgctg catcttttcg gggatattga atacgccgat aatgaattta aacaggaaat
    63541 accacaccac gacggcaatc agcgaaccta acacaaaggc gtgcaggttg attctgagca
    63601 tggtctgtcc gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc aatgccagcc
    63661 cgacggcggc ggcaaacagg acgacaatcc agactaccgt tttcatgcgc gttctccttt
    63721 agcggacggc gaacgccctg ccttttccgg tacgggctgt ttttttgcag gctgttccgg
    63781 tttgcgttcc gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccgg
    63841 ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttctgctt cttgattttc
    63901 cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaggc tgtttttcaa
    63961 accgtcatcc gccgtcatgc gcacatccaa cgccttcaat tccgccagtt ctttcagcca
    64021 cgactgcgtg gcgggagact tggcatcgaa atactgtctg acggcggctt cggcattgtt
    64081 caaatcgccc tgatagactt cgctgttgcg ctgcattaat gcagtccgcg catccaaaag
    64141 gcggaggcgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca gcatggcatc
    64201 gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg atttttccca
    64261 tacgttctgc caccatgaag cagaagcggc ttcgttcttc acctgtacgc ccggcttcaa
    64321 tacgccgtcc aaaatcagcg gcagtccgga tacggcggtt tccagcctgt cgaggcgcaa
    64381 tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaagt cgctgctgac
    64441 tgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc tgtcgatatg
    64501 ctccaatacg ccgactgccg tttggatatt gccggtcaac accagctgtt gcgccgccag
    64561 attgagtatg gtctcggttt cgtccaccag ccaatcggcg cgtcctttgg tcaactcgcg
    64621 ataggatttt tgcatttcca agatttgttc gccgtttgct ttgacgtttc cgtccaaacg
    64681 gtcgagctct gattgtatgg cggcttgccg gttgaggttg tctttcaaca gggcggcgtt
    64741 ttccgactcg cccaaggcgg ctttgtcgat tttttggttg aatgccagct cttggttttt
    64801 caagacattc tgtccttgga caaacaaaaa accacttgta cccaaaccca atgccgccaa
    64861 taccagggcg cagactgcca aagcgttgct gccggactgt ttgacgataa aaggagcggc
    64921 aggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag acggcatttc
    64981 ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt cgcccaccgt
    65041 tttgctcctt attaatttga ggttccggga aagaccatgc cgtctgaaac ggaaatggaa
    65101 gaatgggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac gccttcgcgc
    65161 ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt gaagaatcgg
    65221 gaaaattgcg gcggaagctg tccgaacagc aagcgcacga gttctgtgga cgtaatatag
    65281 gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg ccgtctgaaa
    65341 tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat cagaaaatcc
    65401 cgcccgccgt gtccgcgcac aaacaatacg cgcgcacctt cgggcagact gttccaaacc
    65461 gtcaggcgca aaaccgcctc gctgtcgttg ccgtcttcag gcgcgatgac cgttctgccc
    65521 aaacagcgtt ccaatacgcg gcggctgccc tgccctacgg aaatgtgcat ctttatgccg
    65581 tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg tcggactgac ccaaaacacg
    65641 gcatccgcac gggcatattg ttcggacaaa agttccagtc cggcagcatc tgcttcgatt
    65701 tcaatcgggc tcaacacttc cgcctgccat ccggcacgac ggcaggtttc cacatcgtcc
    65761 ttcgccctgc ccgacggtcg gacaatcagc atgatgggtt tcactgtttc catcgcctta
    65821 tccgccccga ttaccgggaa aaatcagcat cgttgaaatt cgcctgcgcg gtttgcggcg
    65881 tacagccaac ccgtgcgact tccttgcccc tacgctccac aacaacaccg ctgctttccg
    65941 tatgagtcac ggaatcgaag cccgtccata cggtgtacat aaattcgccg ttttggaatt
    66001 tcatcgttgc ccggcgtccg ctacccatgc cttgccacct gtcggaacgc cccaacaggt
    66061 cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac gaatattcgt
    66121 aaagattgcg gttgattttt tgaacttcta tatatttgtt gttgtccgtt ttacaggaaa
    66181 acaccgtatc cgcccacacc gtttgggcgg cagccgacag aagcaataca gcagaaagga
    66241 acattttttt catcgtattt ccttaatggt tgaaactccg ccatttggac atccgtcctt
    66301 cgtggcagca gcatcagatg ttatttgaga ggggtgtaac cccttccaaa ttagggcaac
    66361 acatagggca acgctttatg tgtcgtcctg tgtgttgaaa catcattgcc cgtatcctga
    66421 aatagaaaag aaaatgcccg ttttacggtc ttctttccga caaaatgccg tctgaaacgg
    66481 atttcagacg gcatggtcga aaattattgc gcggcaagcg gcttgagcag caaggtttcg
    66541 ccgctttgca caactttttc aagcaggtcg atattgggat gcttgccggg atgggcgcgc
    66601 gcgtcggcaa cgtgttcggc aaaccatatc aaaccctgtt ctgccgcttc tttgtccaat
    66661 tttccgtcaa aacgttctgc caaagcgttg tacaacttga gcgaaccgag cttaccctga
    66721 acggcaggaa tgtggtgaac cgttttgcca tctgcatcct gaacgtccaa accgcccaga
    66781 tggtcgattg aaggaaatgt ggcgagatag tcttgaaaat tcatatcaac tccgaaaatc
    66841 agtcagttag cgcgtgccga aaatcttgtc gcccgcatcg cccaagccgg ggatgatgta
    66901 gccgttctcg ttcaagtggc tgtcgagcgc ggcggtgtaa atcgtaacgt cgggatgcgc
    66961 gtcgttgacc gccttcacac cctcgggcgc ggcaaccagc accagtgcct tgatgttttt
    67021 gcagcccttg gctttcaaaa ggtcgatggt ggcaaccatc gaaccgcccg tcgccagcat
    67081 cgggtcgata atcagggcag gacgttcgtc catgctgtcc acgaatttct cgaagtagga
    67141 aacgggtttc agcgtttcct cgtcgcgctg cagtccgact acgctgattt tggcagtcgg
    67201 aatcaggtcg agtacgccgt caagcatacc caaacctgca cgcaggatgg gaacgacggt
    67261 caatgttttg cccttgatgc ggtcgccttc aatctgaccg caccatccgt cgataaggta
    67321 tttttcgatt tcaaaatcac ggcttgcctc gtatgccatc aggcgcgcca gctcgatggc
    67381 aagcgtccgg aatttgtagg tgctgcaatc cgcctccctc atcagggtta atttgtggcg
    67441 gacgagcgga tggttgataa cattaacgtt catcgcggtg ttccagtgtg attttgagac
    67501 gcgccattat actttttttt gatttgccgg aaaagaaaat gccgtctgaa acacgttcag
    67561 acggcattgc cgaatccatc aggctttcag cagctcttgc agctcgcctg cctcatacat
    67621 ttccatcagg atgtccgaac cgccgacaaa ctcgccgttc acataaagtt gggggatggt
    67681 cggccagtcg ctgtattcct taatgccttg gcgtacttcg ggattttcca atacgttgac
    67741 ggtaacgtaa tcggtgcagc ctgccgcgtt caggatttgc acggcgcggg aagagaaacc
    67801 gcattgcgga aactgcttcg tacctttcat aaacaatacg acgcggtgtg tcgttactac
    67861 ttctttaatt tggtcgtgga tggaagccat cgcttattcc ttttgataaa gttgacaaaa
    67921 tttgtcaggt aggacgcatt atacgcaaaa gcggaaactt cccacaatat ttgccgtata
    67981 tcgcgcagta tcaatgttgt gaacggaaat attcccactc ctcgacaatc ctgctgtccg
    68041 gcaaatggca cagggcatat ccgccgccgc tttcgtcttt ttttacttcc cgtctgccgc
    68101 ctttggcaat gcagaatttg gaagcggaat ttgccatgcc tgtccgaaac gtgttggcag
    68161 gcagccggca gcagcgcgga catgcatacg gatgaattgc cttcatggtt ttccttatcg
    68221 tcggtacagg gtcagacggt ccgcatatcg ggtacttttt taaacagccg gtaaaagttt
    68281 tccgtcgtat atgccgcaac ctgttccaat gtttggttcc gcaatttggc gatatgttcg
    68341 gcggtatggc gcacaaaagc cggttcgttc tgcctgccgc gtttgggaac gggggcgagg
    68401 aacggcgcat cggtttccac caaaatgcgg tcgtccggaa cgtattttgc cgcctcctga
    68461 accaagggtg cgtttttaaa ggtaacgatt cccgagaaag aaatataaag ccccaaatcc
    68521 attgctgcac gggcaaaacc gatgtcttcg gaaaaacagt ggataacgcc cgaattaacc
    68581 cggcattctt tcaggataga caaggtgtcc gccgccgcat cacgcgtatg gacgataacg
    68641 ggcagtccgg tttgattggc tgcttcgatg tggtctgcaa agcgtttgtg ttgccaggac
    68701 aaatcgcctt tgcaccagta ataatccaaa cccgtctcgc caatgccgac cactttcggc
    68761 gaggcggctg cggcaaccat ttccgcaata gaaaattctt cggcttcctt gctgtcggga
    68821 tgtacgccta tggtgcaata gatgtgttcg tgcgcttcgg cgatggcaaa gacttcggag
    68881 aagctttccc tactgacgct gatggcgagc gcctgcccca cgccgtttgc ttccatgttg
    68941 gacaaaactt cgggcaggcg ttctttcaaa ccttcaaaat tgaggtggca gtgcgaatcg
    69001 atgatatgca tggcgttaca tggtaaacgt aggtctgctg ccgccgattg tgttgcccag
    69061 ttcgacttcg atctggtctt tgactttcag gcagttttcg tgttttgtga atgccagccc
    69121 cacccctttg ggtttggagg aagtacgcgc aggattgatc caggcgactt tggtcggcag
    69181 gaacagtttg gggaagttga ggatttctac ggcaagcaga atatcgtccc cgatggaaaa
    69241 tacgtcgttg gtctgcacaa acagaccgcc gtgttccaaa aacggcatgt aggagctgta
    69301 cagcagattc atgtctttca gctgcaacga catcattttt gccggaatat tttgtccgtc
    69361 tgacataatt cacccgttct ctttcttcaa ttccaaatag ttgatgagca gatgctcgat
    69421 ctgcattttg acatttaaag tatgaaaccc gtagggggca agctgtttga gcatatcctc
    69481 cgccgcaaat acattgcgca ggcggaaacc ggataccgtc tgaagcagcc tgtcttcata
    69541 agcgggataa tagacgggtt tcatgtgttg caggcacaat cccaaatcga ccagccattt
    69601 ctgcatccac ccgacaaata cggcgagcgg aagtttttcc ttatcgaaaa gcgcggcgta
    69661 atccaaaatc ttcaacaacc ttggttctgc caaaatatcc aacagtttaa tccgcaacgc
    69721 acggacaccg tccgcctcat caaacagcgg cgctccggaa tggaaagcca gacgttcctc
    69781 aggttccgcc acaccccttt cacgcagata tgccaatgcc tcttcatggg aaggcgcggg
    69841 caaaaccatc ttccggcagc ggcttttaat ggtcggtaaa accttgtccg ccgcgtggct
    69901 gaccagcaaa aagaccactt gtggcggcgg ctcttccaac actttcaaca aactgttggc
    69961 ggcttggaca ttcatacttt ccgcaggatg aatcagaatc acgcgcaaac cgccccgtac
    70021 cgaagtcagg tacacattat cgatgatttc cctgacggca tcgattttga tctgcaacag
    70081 tttgcgtccg ttttcgggtt cgtccgacaa gggggtgatt tcgtaaaaat cgggatggct
    70141 tccctgtcca aacagatggc aggacataca ttcgccacag ggtttgcagc ccggtgcagg
    70201 ggtttcgcac aacagtgcct tcgccgcaaa gcgggcaaat gtagttttcc ccgtatcttt
    70261 tttgccggca aacagccatg cattgggacg acgctcccaa tgttccgcaa tctgccgcca
    70321 ttgctcatta tgccacggat aaatcatttc aaaccatccg tccggaaatc cataccgcca
    70381 tcagagcagt tccaaatcgg ggctggaact ttgcgcgcgg cggctgcgca actgtaccgc
    70441 caaacgcaaa tcgtgtgcgg aatcggcatt tttcaatgct tcttgcaggg aaatatcgcc
    70501 tttttcatac aattggtaaa ggtgttgatc gaaggtctgc atacccaggg tagtggattt
    70561 tttcatcact tctttgattt catggatgtt gccgttgtga atcaactccg aaatcagggg
    70621 cgaattgagc agcacctcga ctgccgccac cctgcccttg ccgccgtctc gcggaacgag
    70681 gcgttgcgaa ataaacgcct gaaggttgag cgacaaatcc gtcagcaatt gttcgcgccg
    70741 ctcctcgggg aaaaagttga tgatgcggtc gagtgcctga ttggtgctgt tggcgtgcag
    70801 cgtcgccata cacaaatgcc ccgtttcggc aaaggcaatg gcgtagtcca ttgtttcgcg
    70861 gtcgcggatt tcgccgataa ggatgacatc cggcgcctga cgcagcgtgt ttttcaacgc
    70921 cgccatccag ttttccgtat ccacgccgac ctcgcgctgg gtgatgatgc agtttttgtg
    70981 ttcgtggaca aactcgatcg ggtcttcgat ggtgatgatg tgtccgaacg aattttcatt
    71041 gcggtaatcg ataagcgagg caagcgaagt cgatttgccc gagccggtgc cgccgacaaa
    71101 aataaccagc ccgcgttttt tcagcgcgac atccttcaag accggcggca ggttcaggct
    71161 ttcaaacttg ggaatcttgc tggtaatcgc acggaatacc aacgccgtcg cgccgcgctg
    71221 tatcatcgca ttgacgcgga agcggctggt gtccggcagg ctgatggcga agttgcactc
    71281 gttggtcgat gaaaattctt ccgcctgctt cgcactcata atcgaaaagg cgatttccat
    71341 acatttttcc gccgtcagcg gttcgtccgt gatgcgggtg attttgccgt ccagcttcat
    71401 cgcgggcggg aaatgggtcg tcacaaacag gtcggaacct ttgtttttgt tcatatggcg
    71461 cagccatgcg aacagttctt cctttgcggt catattttca tcggtaaaca tcctcgtccc
    71521 ctaatcttca aacaatctgt accttcccat tctaccaaaa acccgccgtg ccgtctgaaa
    71581 ccgtttcaga cggcacggac aaaccacctc cactcaatcc tgccgtgtta gcgtgtataa
    71641 ttcggcattc tatccgaaat tcacagcagg tcagcaatat gtctttgaaa acagtagccg
    71701 ttatcggcgc aatggaacag gaaatcaagc ttttgcgcga gatgatggaa aatatcaaag
    71761 ccgtctcttt cggcagattt tccgcctatg aaggcgaatt ggcgggaaaa cgcatggtgc
    71821 ttgcattgag cggcatcggc aaggtcaacg cggcggttgc aacggcttgg attatccgtg
    71881 aattcgcagc ggactgcgtc atcaacaccg gcagcgcggg cggtttgggc aagggtttga
    71941 aagtcggcga cgtagtcatc ggcacggaaa ccgcacacca cgatgtcgac gtaaccgcat
    72001 tcggctatgt ttggggacaa gtgccgcaac tgcccgccgt atttgtttca gacggcctct
    72061 tggtcggcaa agcaaaacag gcggcggaag tgtttgaagg cgcggcggta gaacaaggcc
    72121 tgattgtcag cggcgaccgc ttcgtccaca gcagcgaagg cgtggcggaa atccgcaagc
    72181 acttccccaa agtcaaagca gtggaaatgg aagcggcggc gattgcccaa acctgtcatc
    72241 agttggaaac gcctttcgtc atcatccgcg cggtttccga ttcggcggac gaaaaggcag
    72301 acatcagctt tgacgaattt ttgaaaacgg cggcggcaag ttctgcaaaa atggtggcag
    72361 aaatcgtcaa atctttataa aacctttatc aaatcatgcc aaaacagcgg ttttccgcta
    72421 gaatgtcggg ctgtttatcc cgttgcgccc gacattcgga cgcagcgccg tttgttcaga
    72481 cggcatttcc aaaacccact gtccgacatt caaaataact ctaatgaaaa atatccgaaa
    72541 tttctccatc attgcccaca tcgaccacgg caaatcgacg cttgccgacc gttttatcca
    72601 atactgcggc ggcttggatt tgcgcgaaat gagtacgcag gtactcgatt ctatggacat
    72661 cgaaaaagag cgcggcatca ccatcaaagc gcaaaccgcc gcgctcaact acaaagcgcg
    72721 cgacgggcag gtgtatcagc tcaacctgat tgacacgccg ggacacgtcg acttttctta
    72781 cgaagtttcc cgttcgctgt cggcctgcga aggcgcgctt ttggtggttg acgcatctca
    72841 aggcgtggaa gcgcaaaccg tggcgaactg ctacaccgcg attgatttgg gcgttgaagt
    72901 cgtgcccgtt ttgaacaaaa tcgacctgcc cgccgccgac cccgaacgcg tggaacagga
    72961 aatcgaagat atcatcggca tcgatgccgt cggcgcggtg caatgttccg ccaaaagcgg
    73021 catcggcgtg gaagacgttt tggaagaaat cgttgccaaa atccccgcac cggccggcga
    73081 tgaaaatgcg ccgctgcaag cggtgattgt cgattcgtgg tttgacaact atgtcggcgt
    73141 ggttatgctg attcgtgtga aaaacggcac catcaagctg aaagacaaag tgcgctttat
    73201 gagcaccaag gcggaaacgc aggttgagca gctgggcgta ttcacgccga aatcagttca
    73261 aaaacaagaa ctcaaagccg gcgaagtggg ctttttgatt accggcgtaa aagaattggg
    73321 acaggcaaaa gtcggcgata cagttacttt gattgccaac cccgcctccg agccgctgcc
    73381 cggcttccaa gaagtgcaaa gccaggtatt cgccggactt tatccggtgg aaagccacga
    73441 ctacgaagcc ttgcgcgacg ctttggaaaa attgcagctt aacgatgctt cgttgaaatt
    73501 cgagcctgaa gtttcccaag cattgggttt cggcttccgt tgcggcttct tgggcctgtt
    73561 gcatttggaa atcgtgcagg aacgtttgga acgcgagttc gacatggact tgattaccac
    73621 cgcgccgacg gtggtttatg aagtcgtgtt gaaaaacggc gagaaaatcg aagtcgaaaa
    73681 tccgtccaaa ctgcccgaca tcggcagcat cgaaaccatt ctcgagccga ttatcactgc
    73741 aaccatcctc gtgccgcagg aatatgtcgg caacgtcatg actttgtgca accaaaagcg
    73801 cggcgtgcaa gtcaatatgc aatatatggg ccgccaagtg atgctgactt acgacttgcc
    73861 gatgaacgaa gtggtgatgg actttttcga caaactcaaa tctacttcgc gcggctatgc
    73921 ttcgttggac taccatttca aagagttcca accgtctgat ttgattaagc tggatatcat
    73981 ggtcaacggt gaaaaagtcg atgcgctgag cctgattgtc caccgtcaaa gcgcggttca
    74041 ccggggccgc gaactggcat cgaaaatgcg cgagctgatt ccgcgccaaa tgtttgatat
    74101 tgccgtccaa gccgccatcg gcagtcagat tatcgcccgt gagaacgtca aagccctgcg
    74161 taaaaacgtt ttggcaaaat gttacggcgg cgatattacg cgtaagaaaa aactgcttga
    74221 aaaacaaaaa gcaggcaaac gccgtatgaa acaagtgggc aatgtggaaa tcccgcaaag
    74281 cgcgttcttg gcaattttgc aggtgagcga caaataatat ccggcacacg gggcagtgca
    74341 tcatgcccct taaaccgccc tgattgtaag agagcacgtc cgccaacaaa ctttgtgatt
    74401 taccgcctct taaaaacggt tgcacggacg ggcgggtttt taaccgttaa ggaacaacaa
    74461 tgaacattat gctaatgtcg ggcgcggctg ccgcgctgct tgccggcatc atcctttatt
    74521 tcaaaagcga caaaaagcgg caggaaaacg gtgaatggag ttccggtctg gaatacgcct
    74581 atatcctgac ggcggtcggc gtgtttgccg ctttgtccct gtttatgagc tttaccgccg
    74641 ttttcctgat tttcgttgta ttgtgcggta cggcttgggg ggtatacaaa taccgcctga
    74701 agactcatcc cgaaatctcg gaaagcagcc acttcggcga ttatttcggc agtttcttcc
    74761 ctaccgtttt ggtattgttc ctcatccggt cgtttattgc cgaaccgttc caaatcccgt
    74821 ccagctcgat gcgcccgggc ctgattaagg gcgatttcat tttggtcggc aaattttcct
    74881 acggcctgcg cgtacccgtt ttaaacaatg tattcatccc cacgggcaaa atcgaacggg
    74941 gcgatgtggt cgtttttaat tatcctttgc aaccggagat gacctacatc aagcgtattg
    75001 tcggcattcc gggcgatgtg gtcgaatatc ggaataaagt tttgacggta aacggaaaac
    75061 ctgcttccga tattcctgac ggcacatacc gttatcccga cgacaccgac ccatccgaaa
    75121 tccacaatac ggatatgttc cacagcggtt tggacggcaa atccttcaat attctgaaaa
    75181 aagaaggaca gcctgccgtt tccctgcccg tattgggcaa atatacctcc gatattatgt
    75241 ctgaaaacgg atattccata gagcaaagcg gtttggaaca ctgccaatat gccgacgacg
    75301 gcagcggttt cgtgtgcaaa gttcccgaag gacgctattt cgctatgggc gacaaccgcg
    75361 acaacagtgc cgattcgcgc tactggggat ttgtggatga caagctggtt gtcggcaagg
    75421 caatgttcat tttgatgaac ttcggcgatt tcggcagggc cggtacggca atccgttagg
    75481 ccaggaagcc aagaatgccg tctgaaaata gtttcagacg gcatttgcta ttcctcactt
    75541 ttgcaggcgg atctaacgtt gtcggatttt cctacctata gtgaattaaa tttaaatcag
    75601 gacaaggcgg cgagccgcag acagtacaaa tagtacggca aggcgagcca acgctgtacc
    75661 ggtttaaatt taattcacta tatcagtccg gtaacgcagg ttttgcacgg aaaactgcca
    75721 atactgcctt gatggaatca atattaagac tgctgtttca gcgaaccgag gtaaattgcc
    75781 gaaagcgtca gcgccacccc tgcccactgc acgccttcaa tcggtttgcc caacccgaaa
    75841 taatcgatga acagggcggc aaccggttcg gacaaaagca gcagccccgt cagcgacagc
    75901 gaaagcagcg gaatcgcata ggcaaccatc gcccacgcga agcactgcat caccacgccg
    75961 tacacaagca ccaaacccgc atctttccaa gtcgtcggat aaagcacgcc gccatccatc
    76021 agcaatgccg gaacaaccag cgataccgcg ccgcccaaac tcaaaatcat cattgaaggg
    76081 aaaagcgcca ccggctcgat ttcatgggtt ttgcggacga acaccatcga cagggcgagc
    76141 atcagtcccg ataccaaacc gctggcgaat ccccaaaccg cattaccgtt gtagccgaat
    76201 tccgcaccgg caatcatcgc cacgccggca actgccgata ttaagcctgc ctttttcagc
    76261 ccgctcaaac gctcgccgaa aaagaaaaca ccgattgccg acaagaaaaa gatttgcagg
    76321 ctgttgagca gggtggaaat acccggcccg accgcgtgta tgctttcgtg ccacaacgcc
    76381 aaatcgaaag cgagaaacac gcccgccgtc agggcatatc ggacggtttt cctgtttttt
    76441 gggaattttt gcccgaaaaa ccgtgctaaa aaccaaaata cgaacaccga aatcagcaac
    76501 cgccaaaatg cgattgcata cgaaccgacg gggacggatc tgacaatcag actgcccaga
    76561 ccgaacacca cgcaacccag caccagcaat ggtgcggcat attttttgcc gttaccgacc
    76621 acaatatccc ctcccttccc catacggctg ccgcgccgac ggcaagaatg ccgccattcc
    76681 gaaaatatgc cgtctgaagc ccaaagcccc ggctgcaccc ggacaaacgg ctgattttac
    76741 gggcaggcag gctattttcc gttggggcaa aaaaccgtcc tgccggataa aacccgaata
    76801 tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct
    76861 ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat agtggattaa caaaaaccag
    76921 tacagcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcat tgccttgtcc
    76981 tgatttttgt taatccacta tatctgtact gtctgcggct tcgtcgcctt gtcctgattt
    77041 ttgttaatcc actataaaaa atatttccct caagcgatgt ggcggcacca acacctgatt
    77101 aaaacacaac cggcgttttc ggcatatacg caaaaacgca ttttccggca cggcgttaca
    77161 ccgataccga aaaatgcgtc tgaatgtttc gacaatccga ttaaagcatt atgccaaatc
    77221 tgcaaaaagt ggcgtagaga gatagcgttc gccataagaa ggcagcagca cgactatcag
    77281 cttgccttcg ttttcaggct gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga
    77341 agaaataccc accaaaatgc cttctttttc cgctattgcg cgggcggttt caaaagccgc
    77401 ttcgttcgac actttggtaa tgctgtcgta aattttggta ttcaaaacgg tcggaataaa
    77461 gcctgcgccg atgccttgaa tcgggtgcgg gcctttttcg ccgccgctca atacgggtga
    77521 agcttcaggc tcgacggcaa ccactttaac ttcgggtttg tattttttca acacttcgcc
    77581 cacgccggta atcgtaccgc ccgtgccgac gccggcaacg aagacatcga ctttgccgtc
    77641 cgtatcccgc caaatttcct cggcggttgt tttgcggtgg acttcggggt ttgcctcatt
    77701 gtcgaactgg cgcggcataa aataagtgtc gggatgcgcg tccaccaagg atttcgcttt
    77761 ggcaatcgcg cccgccatac cttcggcggc aggggtcaga atcagctccg caccaaacgc
    77821 gcgcaacagc attttgcgct ccttactcat gctttccggc atggtaatcg ccagcttgta
    77881 gccgcgtgcg gcacatacca ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac
    77941 aatgacggtg tttttgttga ttttgcccgc tttttcggca ccctcgatca ttgcttcggc
    78001 aatgcggtct ttgacgctgc tgcctggatt gaaaaattcc agtttcacgg caacctccgc
    78061 cttcaaacct tcggtcagac ggttcagttt gaccaaaggc gtgttgccga ttagttcggt
    78121 gatgctgttt gcaattttca tcttctgctc ctgaataata aggtttcgac agtaaggcgc
    78181 aatccccata ggcatcctgt ccgattgcag gggatttgcg ccgaaacata aacaaatttc
    78241 ggatatggct ttatcctaaa cctatgccgc ctaaaaaacc aatatccaaa atttctttct
    78301 ttataacttt ttgttccgat acggccgcac cgtattatct tccaatttct gaaagcagca
    78361 gcaccagtac cgagacctgc atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc
    78421 cgccgaacgt tgacggcggc tgccggtcta taccgcagcg gtggacatag gcaaccgcca
    78481 tcagcacaaa ctgcaacacg aaccaataaa ccggcttcag cgcataaagt ttgagcagtg
    78541 ccgcaaacac aaacacttga accgttcccg aaaagcaata atgcaccaac gcacatattc
    78601 cgacaaaagc aagcgtcatc gatacattac tgacggcaaa cagcccgagc agaggatgtt
    78661 cggggtctgc ctgccatccg tttccatccg ttttccggtt ccaatgcccg atgaaaaaaa
    78721 atatgctgcc caaagtcagg taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc
    78781 cccatatgcc gggcatcagc tttgccccca aaaccgatat gcccagccac agcataatac
    78841 tcgcccaaaa ccattgcaac attcctgcgc ggaatatcag aaacagaaaa accgccgcat
    78901 acagtaaggc agacaaaaaa tccaaatgca gcaaaatcat gaatacgcca actcgccttc
    78961 aaacaccgtt tccgcagggc cggtcatcat cacatcgccg ccgcaggccc attcgatata
    79021 taaagttccg cccggcaaaa tcacctctac cgttttccct tcatccaaca accccagacg
    79081 gatacccgcc accacagccg cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc
    79141 gcgctcgaac acgcgcaaac gaatcgcggt tcggctgata atctgcataa agccgacgtt
    79201 gacgcgttcg ggaaactgcc tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc
    79261 gcattctacg tcatcgacca caatcaccgc atgggggttg cccatattga cgcagctgac
    79321 aggctgaatg ccggattcga gatgcacccc gtaaatacag gcatcatccc cctcgcccga
    79381 ttcggggaca aacggtattt cagacggcat aaacctcggt ttgcccatat tgaccgtaac
    79441 catgccgtta tcggacaatt tcggaaaaat aacgccattt gccgtttcaa cacaaatttc
    79501 tttcttatcg gtcaaaccct tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc
    79561 gcattgctcg acctcgctac cgtcggcatt gaaaatacgg taacggaaat ccacgccttc
    79621 ggtttccgaa cgcccgacca ccaaaagctg gtcgaagccc acgccccgga agcggtccgc
    79681 ccatgccgca atcggcgcgt cctcgggggt aaaatcctga ctgaccgcgt caatcaccat
    79741 aaaatcgttg cccaaaccgt gcattttggt aaatttcaaa gtgttcatat tgttccgaaa
    79801 tcccgtatcg tctgttgctt ccgaatccga aatcccccgc accgaaggga aatgcttcca
    79861 tctcccgccg cctgaggatt aaaatcagcc attatactgt ttgttacccc ttacaagaaa
    79921 caccatgttc cgcatattac tgttgctttt cccttgtgca atccttgccg cctgcgcccg
    79981 aaacataccg gcaagccccg ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct
    80041 gtattttgac gcacacggca aacccgccga cggcgcagta aagggcggat tttacaaaat
    80101 tatcgaaata cagggtgcaa actgcatcct tattcaagac tttaacagca cgggcaatgc
    80161 cctcggcgga tcctatgtgc cggactgccg caaatcatcc cttttcccga aaaatatttg
    80221 acttcaaact gcctatgaac cacgacatca ctttcctcac cctgttccta ctcggtttct
    80281 tcggcggaac gcactgcatc ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac
    80341 tccccccgca tatcaaccga ttttggctga tcctgctgct taacacagga cgggtaagca
    80401 gctatacggc aatcggcctg atactcggat taatcggaca ggtcggcgtt tcactcgacc
    80461 aaacccgcgt cctgcagaat attttataca cggccgccaa cctcctgctg ctctttttgg
    80521 gcttatactt gagcggtatt tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga
    80581 tatggcggaa cctgaacccg atactcaacc ggctgttacc cataaaatcc atacccgcct
    80641 gccttgcggt cggaatatta tggggctggc tgccgtgcgg actggtttac agcgcgtcgc
    80701 tttacgcgct gggaagcggt agtgcggcaa cgggcgggtt atatatgctt gcctttgcac
    80761 tgggtacgct gcccaatctt ttggcaatcg gcattttttc cctgcaactg aaaaaaatca
    80821 tgcaaaaccg atatatccgc ctgtgtacgg gattatccgt atcattatgg gcattatgga
    80881 aacttgccgt cctgtggctg taagccccat tcattcccga tacccgtaaa aaataccgtc
    80941 tgaaacctgt tcagacggca ctttcaccct aagcgttact cggcggcatt ttcctcacgg
    81001 gcaggcgcgt ccagcagggc tttgatggac agacggacac ggcctctgtc gtccacttcc
    81061 aatgctttca cgttcaccac ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc
    81121 tcgtgggcga tttggctgat gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc
    81181 gcgccgacgt tgttgtcgag gattttcacc acagtgcctt catacacttt gcccacttcc
    81241 acttcggcag taatctgctc gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtc
    81301 gcggcaatgg taatcgtacc gtcttcggca atattgattt ccgtaccggt ttcagcggta
    81361 atcgaacgga tggtttcgcc acctttaccg ataacttcgc ggattttgtc ttggttgatt
    81421 ttcatcgtaa acaaacgcgg cgcgtgtgcg gacaactctt gcgggcccgc aacggcggct
    81481 ttcatctgat ccaagatgtg cagacgcgct tctttggcct gtgccaaagc gatttgcata
    81541 atttctttgg taatgccttg gattttgatg tccatttgca gcgcggtaac accttcggtc
    81601 gtaccggcca ctttaaagtc catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc
    81661 aggacggcaa atttgttgcc ttccagaatc agacccatcg cgataccggc aacgtgtgct
    81721 ttcaaaggca cgccggcaga cagcaggctc aaacagccgc cgcagacgga agccatagag
    81781 gaagagccgt tagattcggt aatttcggag accacgcgca tggtgtagct gaaatcttca
    81841 ggtttcggca atacggccaa caatgcacgt ttagccaaac gaccgtgacc gatttcacgg
    81901 cgtttcggcg cgcccatgcg gcccacttcg ccggtagagt acggcggaaa gttgtagtgc
    81961 agcataaagc ggtcggtgta ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa
    82021 gtacccaaag tcgctacggc aagggcttgg gtttcgccac gggtaaacaa tgcggaaccg
    82081 tgcgtgcgcg gcaatacgcc ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg
    82141 ccgtcgatgc gcggttggcc gtccaaaatt tggctgcgga cgacatcggc ttccaagtgt
    82201 ttgaaaatgc ctttgatttc gttggctgcc agcgtgtcgg tttcttcggt aatcaaggct
    82261 tcttttaccg cactccaagc ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt
    82321 ttgaacgctt ctttaatggt ttcgccggca atcccgcgga ctttggcaac cagttcctca
    82381 ttggtttcag gtgctttcca atcccaaact tccggattga cttcgtcggc aaattcattg
    82441 attgcattga tggcaacctg catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg
    82501 tcttcgggca ggattttggc ttcggattcc accatcaaca cggctttgga agtaccggcg
    82561 accaccaagt ccaattgcga tttcgccaat tcggctttag tcgggttcaa aacgtacacg
    82621 ccgtttacat aaccgacgcg tgccgcgccg atcgggccgg caaacggtac gccgctcaac
    82681 accagcgcgg cagacgcgcc caacattgca ggaatatcag aatcgatttc aggatcgacg
    82741 gacacgacca tcgctacgat ttggatgtcg tggtagaaac cttcagggaa cagcggacga
    82801 atcggacggt cgatcagacg gctggtcagg atttcttttt cgctttgttt gccttcgcgt
    82861 ttgaagaaac cgccgggaat tttgcctgcg gcgtaagtgc gctcgaggta atcgacagtc
    82921 agagggaaga agtcttggcc ttctttcact tctttgttgg tggtaacggc aaccaaaacc
    82981 acggtgtcgc ccatagagac tttaacggca gcggcggctt ggcgggcaat ttcgccggtt
    83041 tccaaagtaa cggtgtggtt gccgtattgg aaagttttaa cgtgtttgtc gaacattatt
    83101 gttcctttca aaataccgca ctgctaaaac actaataatg cacactaaaa tccgaatgtg
    83161 catagttagg gtttcagacc gtgcggcagg ttgtaaacaa gctttcagac gacatttcgg
    83221 acgctgaaag caattccaga ttataaaggc aaccatcctt aaaatccagt attgatacaa
    83281 taaaaaggcc gtctgaacca tatttctttc agacggcctt caaacttaaa aatcaatcct
    83341 gagtcagcaa agtcaatgct tcccgatact tctcgacagt tttctgaatc acatcggcag
    83401 gcactttcgg cgcaggggct tttttgttcc aaccgctttg ttccagccag tcgcggacga
    83461 attgtttgtc aaaagacggc ggattggtgc cgactttgta ttggtcggta ggccaaaaac
    83521 ggctcgaatc gggagtcaat acctcatcca tcagcgtcag cgtaccgttt gtatccaaac
    83581 cgaattcaaa tttggtatcg caaataataa taccgcgcga tttggcatat tccgccgctt
    83641 cggtgtaaag ccgaaccgcc ttggcgcgca cttcttccgc caattctttg ccgataatgc
    83701 gtccgcattc ttcaaagctg atgttttcat cgtgatcgcc gactgcggct ttggttgagg
    83761 gcgtaaaaat cacttcaggc agttgttgcg cttcctgcat accttcaggc agttgaatac
    83821 cgcaaaccga gccggttttt tgataatctt tccaaccgct gcctgccaga taaccacgca
    83881 caatcgcctc tactttcacc ggagtgagct ttttagccac gacggcgcgt ttctctaaag
    83941 ctttggcttc gttttcaggc aaaacatcgt aaaccgtttg accggtaaag tggttgggca
    84001 taatatgcac cagtttttta aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc
    84061 tcggaatcgg gtcgtccaaa atcacatcga atgcagacag gcggtcggaa gcgaccatca
    84121 gcatacgttt atcgtcgatt tcatataaat cgcgcacttt tccaaaatag atctttacca
    84181 aaccaatctc actcatttcg ccccccctga aaatatcttg aaaataccga cccgacaccc
    84241 gacaggtttg aatcacaaac caatattcta gccgaagtcg gcgcaaaaca atacccatgg
    84301 cacaaaaaac caacccgtca accgtcggca aagttttggc actataatac cgacagcaag
    84361 tcctacaata cacttttacc aaaggaaata cctcatgaga atcctattga caggctcgaa
    84421 aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa gactgggaaa ccattgcgac
    84481 ggattccgca tccctagaca ttaccgatgc cgatgccgtc tgcaacatgg tcaaaagttt
    84541 ccaacccgac gccattgtca acacggctgc ctatactgcc gtcgacaaag cggaaggcaa
    84601 tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac aaccttgcct tggcagcaca
    84661 tcgcgcccat gcccgattca tccacatctc aaccgactat atctttgacg gtaaagggaa
    84721 aaggccctat caggaaagcg actttaccaa tccttccaat gtatacggac aatccaaaac
    84781 cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc cttatcctgc ggacttcttg
    84841 gctgtttagc gaatacgggg acaactttat ccgcacgatg ctgaaccttg cgcgggaacg
    84901 ttccccgctg tccgccgtcc acaaccaaat cggctgcccg acctatgccg gcgacttgtc
    84961 cgccgccatc atccgcctgt tgcagcagtc caatcccgtt cgcggcattt accactacgt
    85021 cggcggcaaa tccgtatcct ggtacgaatt tgcccaacat attttccaag cggcatcgca
    85081 acagcagaca tccttccccg ttcccgaatt gactgccgtt tcagacaagg aatatccgac
    85141 cgccgccccc aggcccgcat acagcatttt ggactgccgc aaaatcgaaa acgacttcgg
    85201 catcaaaccg tcagactggc aaaaagccct tgcgcaggtc gtttccaagc tgctctgatg
    85261 ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg cggttttctt atatagtgga
    85321 ttaacaaaaa ccagtactgc gttgcctcgc cttgccgtac tatttgtact gtctgcggct
    85381 tcgtcgcctt gtcctgattt ttgttaatcc actataaaat ctaccgatta cacaaacaca
    85441 tatcatcttt acacaatcat gcttccatca acagtaaaac atgatatgat tgccaacaat
    85501 aacatctcac aataaatttt ctaattttta ttgaaaaaat caataaataa gaattctccc
    85561 aaccgacaaa taaaataaag aaagggtcaa tatgcaacac cgaagaagac tggcaattta
    85621 ccaggcatcc aaacgtgctt cctttactgg caggtcatcc gccccgcaaa aacgtaaaga
    85681 acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca gacggcattt ttttaccgtt
    85741 cgagaaactg tttcaacctg tcctcatcca aaaaccagtg gcagatttcc tcatctcccg
    85801 ccaacaaaac gggaaccagc tcattgtatt tttcttccaa aacaggattt tcatccacat
    85861 cgaccacttc cagcccgaac ccgtattcat tttgaaaagg tttgagtgca tcgcgcattt
    85921 tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa tttcatccgc gtttccttat
    85981 ctgtcaattt gcacacgcca aagccttaga cgcagcagaa tcatggtcta tttgggaaaa
    86041 aacaatgttt tcaaggaaga tgatactcaa gtcctgccaa aaacagcaaa aatgccgtct
    86101 gaacagttca gacggcattt ccaaaaccgt tttacgcttg aacattgata cccgccgtaa
    86161 ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc aagctcccca cagcagccgc
    86221 agcagctcgc tacgcccaat tgcgactgca aatcgcccat tgtggtcgcg ccggcggcga
    86281 tggtttcctt gatttgatgg tcggtaacgg cattgcagat gcagacaaac attttgtgct
    86341 ccgtgtgttc tcaaactatc gtaccgatag cggcttttat tatcgtatgc gaatataaat
    86401 aaaaacggtt cgcattgcaa ggtcggtata cacggtttgt cttggtaatt ttttatcaag
    86461 tttgcatttt cgattgattt cttattaaac caaagtagaa gcctcagttt cgggaatggt
    86521 gttccttcac tacaccctgc aaccatacgt tggcaacatg ggtataaatc tgcgtcgtat
    86581 tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa atccaagccg tgccgcacca
    86641 gatgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat gtgcccgatg cctgcctgac
    86701 ttgcatattc tttgacaatc atccatgcca actgacggga aatgcccgtc tttttctgac
    86761 tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag tgggcgtgcc tccgtataat
    86821 agcgttccac ccaatacgcc gactcctgcc ccatcgggac catcctctgc ttatcaccct
    86881 ttcccagcgc ggtaatacag cccctgtcca aatccacatt gccgaagttc agcccgaccg
    86941 cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag caaagccttg tcccgcaaac
    87001 cgtgcggcgt gtcggtatcc ggggcggcaa gcagtcggga aatctgctgc tcggtgatca
    87061 gggtcggaat attcttgtcg attttgggcg gtttcagcaa acgggtggga ttgtccgtcc
    87121 ttatgccttc acgctccatc catatataca ggcgtttgca tgccgataat gcgcgcgcct
    87181 gcgaactccg ttgctctccg tcaacataaa ccgccgccgc caaatccgct tcgtccgcat
    87241 ccttcagcat tctgcccgat tgggacaggc ggcgggcgat tttttccaaa tcgcgccggt
    87301 aaccgtttaa agtattctga ctgagccgcc tgtccaacca cagcgtttca agcagcctgt
    87361 cgatcaaacc ttcttccata ccgttccaaa caaatgccgt ctgaatcttc ttcagacggc
    87421 atggttcaca ttatcgggaa agcgtttcca atacttcctg cgcgtgaccc gccactttga
    87481 ctttccgcca ttcatgggcg atttctccat ccttattcaa gacgaacgta ctgcgctcga
    87541 tacctaacga ctctttcccg tacagtttct tcaatttgat gacatcaaac aggcggcaca
    87601 ctgtttcatc cttgtcgctc aacagctcga accggaaacc ctgcttggcg caaaaattct
    87661 gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt ataacccaat gcctcaaact
    87721 gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca gcccagcgta ctgtctttcg
    87781 gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg aaaatccgca ccgctgctcg
    87841 aaggcagggt aaattcatat ttcacatcca tagtcctact cccgatattc ccattattca
    87901 aaacggcacg cagacgcacc gccgcaattg ccaaaccaac cccgattcta ccgccccaaa
    87961 ggacaaggat tcaaccgccg gaaacatcca aaccgacaca cgacggcatg aaaaatatcc
    88021 atgtcaaacc acaaaatatg ttccgattta aaaacagaat gttataaaac ccaatcccca
    88081 aaacacaaca agaccgcccg cctacgggca gtctcctgtc agacgacata ctttacagat
    88141 ggctgttttt tcaacaaaat aacgccaata ctcaaaaata tggaatcaaa aatgtccatc
    88201 catactctga aacgcctgcc ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca
    88261 gcccaccttt ttgccgacaa cgacatttta gggcaatttt tagaacagaa catgcttacc
    88321 tcctccgatc cgatagaaat attcgccgaa agcacgatac accccaccaa cacccaagcc
    88381 attacaggcg gtctgattct ctcctcacag tctgccctgg tcgtcaacaa caaaaccgga
    88441 cagatactgt atcagaaaaa cgccgacagg attatgccca tcgcctccat ttccaaactg
    88501 atgagcgcga tggtcgtttt ggatgcaaac ttggacatga acgaaaccgt taccattacg
    88561 cccgacgaaa tcgaccgcat caaagggacc ggcagccgtc ttgccatagg tacggcactt
    88621 acacgcaaag aactgctgca cctgagcctg atgagcagcg aaaaccgcgc cacccatgca
    88681 ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc
    88741 caaagcctcg gtatgtacgg cagccgcttt tacgaaccga ccggactcaa cttccaaaac
    88801 gtttccaccg ccaaagacct gagccttatg gtcaacgccg ccgcccaata tccgcaaatc
    88861 cgcaccaact cgacttccaa ctacgcctcg gtacagacca aaaacgggca gcagaactac
    88921 aaaaactcca atgcgctggt cagggaaggc atgtggaaca tcgaattgca gaaaaccggc
    88981 tacatacgcg aagcaggcag gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt
    89041 accatcgtat tgctgaactc gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc
    89101 gaatcgtgga tgctgcagca acgctcctga catacaaatg cccggcggaa aaccgcccaa
    89161 acacaaaaaa aattggtaat ttttcctatt caaggctaca attcgacacg cacagggcat
    89221 ctgcccgatt tcacaatcat aatagcggag ttaaaaatat gacagctaaa ggacaaatgt
    89281 tgcaagatcc cttcctgaac gccctgcgta aagagcatgt tccggtttcg atttacttag
    89341 ttaacggtat caaattgcaa ggtcaggttg agtctttcga tcaatacgtt gttctcctga
    89401 gaaacacttc cgtcacccaa atggtttaca aacacgccat ttccaccatc gtaccggcac
    89461 gctccgtcaa cctacaacat gaaaacagac cccaagccgc accgacttcg accctcgtcc
    89521 aagtggaaac cgtccagcag cctgccgaat aatccgcacg aagcatgacg tgtcatatct
    89581 ttcaatacct taccggacaa cggtaaggta tttttatttt cagacagcat ttaaaaatgt
    89641 tattgcaaaa catccttcca ttcgcccatt gccttttgcg gaaggcactt cccgaaggcg
    89701 gcaatgcttt ggacggcacc gccggcaacg gacacgacac ccttttcctc gcacaaaccg
    89761 caggcatccg ggggaaagtg tgggcattcg acatccagcc gcaagccctg agccgcactc
    89821 gatgccatct gaaagaagcg agatacgaga atgtgcaact catcttagac ggacacgaaa
    89881 acctgaaaca atatgtttca gaaccactaa atgccgccat tttcaacttt ggctggctgc
    89941 ccggcggaga taaaagcctt accacccata cggaaaccag cattcccgcc ctttgcgcaa
    90001 ccctctccct gctgaaagaa ggcggtatgc ttattgccgt cctctatccg ggacacgaaa
    90061 acggcaaaca ggaggcagaa gcaatcgaac aatgggcaaa aaacctgcct caagaacagt
    90121 ttgccgtttt gagttacggc tttaccaacc ggaaaaacaa cccgccctat ctgttggcat
    90181 ttgaaaagtt acgtcaaaaa taaatgtttg cggtaaaata agccgattct aacccttatt
    90241 ttgatacgag ccgaccatgc agtatctgat tgtcaaatac agccatcaaa tcttcgttac
    90301 catcaccatt ttggtattca acatccgtgt tttcctactt tggaaaaatc caaaaaagcc
    90361 cttggtcggc ttttggaagg cactgcccca ccttaacgac accatgctgc tgtttacggg
    90421 attgtggctg atgaaaatta cccatttctc cccgttcaac gcgccttggc tcggtacaaa
    90481 aatcctgctt ctgctcgcct atatcgcatt gggtatgatg atgatgcgcg cccgtccgcg
    90541 ttcgaccaag ttctacaccg tttacctgct cgccatgtgt tgcgtcgcct gcatcgttta
    90601 ccttgccaaa accaaagtcc tgcctttctg aaacaccgtt atgaacaaca gacattttgc
    90661 cgtcatcgcc ctgggcagta atcttgaaaa ccctgcccaa caggtacgcg ccgcattgga
    90721 cacgctgtcg tcccatcccg atattcggct tcatcaaacc tcgtccctgt atgccaccgc
    90781 ccctgtcggc tatgacgacc agcccgactt tgtcaatgcc gtctgcaccg tttccaccga
    90841 tatggacggc atctcgctgc tctccttgct caaccgtatc gaggcggatt tcgggcgcaa
    90901 acgcagtttc cgaaacgcgc cgcgcacatt ggatttggac attatcgact ttgacggcat
    90961 ctccagcgac gacccccgcc tcaccctgcc gcatccgcgc gcacacgaac gcagtttcgt
    91021 catacgccct ttggcagaaa tcctccctga ttttgtttta ggcaaacacg gaaaagttgc
    91081 cgaattgtca aaacaactgg gcaatcaagg tatccgtctt ttaccggaca ggtaattccg
    91141 cacacagatg ccgtctgaat gccggtttca gacggcattt ttaccctgcc gccaatctgt
    91201 acgcaaaaaa aatcgcccct tgaaaaaggg gcgcaaaagg aacacaaacc actaccaaaa
    91261 ctttaaatct gcaacactgc ctgccgcata ctgtatccga caggatataa agccctcact
    91321 aaatcgtttc gagaaatcca aacttcttca tcgccgacag aaaatctgcc tttctccggc
    91381 accagctcca acaaaaacgg ttgaaccgcc gtatgcagcc tgtccttaca ccgccccagc
    91441 tttctggaca acgcggacaa ggcacgctta tagacactat ccttgcggtc aagttcccgc
    91501 gcactgccga cccagctcaa acgaaggttg cggctttcat cctcaatcag caaaatgcct
    91561 gttttgactt cccccacctc gggctcacat gaaagcgcaa tataatattt gccgtcgatg
    91621 ttgacatacg ccgcttcatg cacctgacag ttgcagccgg aagtggcaag cagcgaataa
    91681 acagagtccc gatgcagttt caaaatatca agggaaatct gtttgaaaat actttccggc
    91741 atttttccct cctttcgtct gctgcgttgt ttactggatt gactgcatta tacagacatc
    91801 tcccaatctt gcaaatgata attgttccta ttttttaaaa aatttataaa ttattgatgg
    91861 atatgtgaat taattttaag atttaaaatc tctttctcaa aaaaacacaa aaatgccgtc
    91921 tgaaacgtgt tcagacggca ttggctgcac agccagtccg tttaaaattt cacgccctta
    91981 tgcagcgcga cgatgcccgc actcatattg tgataatcca cgctgtcaaa gcccgcatcc
    92041 agcatcatct gtttcaaagt ttcctgatcg gggtgcatac ggatggattc ggcgagatat
    92101 tgataactct ccgcatcttt ggcaatcagt ttgcccatga ccggcagcag cttgaacgaa
    92161 tagaaatcat acgcgccttc caaaggtttg tagattttgg aaaactccaa taccagcaat
    92221 gtcccgccgg gtttcaaaac acggtacatc tctttcagcg cggcatcttt atgcgtcata
    92281 ttccgcaaac cgaacgccac ggaaaccaag ttgaaataat tgtcagggaa aggcagtttt
    92341 tccgcatcgg caagcgacac gggcaaaatc atgccttcgt tcaaaagacg gtcgcgcccg
    92401 acggtcagca tagaggaatt aatatcggtc agccaaacct cgccttcctt gccgacccgt
    92461 ttcgcccaac cgcgcgacaa atcgcccgta ccgccggcaa tgtccaacac tttatcgcct
    92521 tttttcaggt gcgccgtgtt gatggtgaaa tgcttccaca ccctgtgcag ccctgccgac
    92581 atcacatcgt tcataatgtc gtagtttttg gcgacggagt ggaacacttc cgccactttt
    92641 ccggctttct catcttcgtt gaccgtactg aatccgaaat gcgttttctg tccgcccata
    92701 ttctaaccac ctccctgatg ccgccctacg gcatcccaaa cttaacaatc tgattgcttt
    92761 tcaaaacggg aagcacccgc cgccgccaat ttgtccaaat attcctgcca catcgcatcg
    92821 tatccgtatg ccagtctgtg cagatacgcc caatcgtaaa gaccgctgtc gtgcccgtct
    92881 gaaaaactga ttttcagcgc gtactgtccg acaggctgca aatccgcgat ttggacatcc
    92941 gccttgccgg tctgcaaaac atcctgtccc acgccgtgtc cgcgcacttc cgcactcggc
    93001 gaatagacgc gtaaaaattc ggcaggcaga tttttaggtt cgtccccata agtcaaaacc
    93061 aacacggtac ggtttttcaa caaacggatt tcaacaggaa tattattcaa aaaacacctc
    93121 ggttcggcat acaatatccg gcaggcgcag gcgtgccttc aaacggcgcg tattttaaca
    93181 gctttatccc gataccgata ttcaaaccca cactttaaac ggcattgata tggacaatct
    93241 cgtatgggac ggcgttcccg acatccgcac actcgaccaa accatccgca aacacgcaca
    93301 cccgctcaac ctgattgtct gcctccccga taatcagatt cccgattttc aaaccgcaca
    93361 agatgcttcg gacgcggaat gccgtctgaa gcaccgtttg gatcaggcaa cgcagtgcct
    93421 ccagttcgac agcatcaacc tcatcgaaca catcctgccc gatgtccgct tctggctggt
    93481 tcccccttca cgcacccgcc gcctgcacga acacttccac cacatttcct ggcagaccga
    93541 agccatcccg caaaccgaaa gcaaacccga caaaccctgg tttgcacttc cacaaacatc
    93601 cgaacggcaa aaaccggaac acatcctcgt tatcggcgcg ggcatatccg gcgcggcaac
    93661 cgcccacgcc ttagcatcac acggcatttc cgttaccgta ttggaagccc gaaaagccgc
    93721 ccaagccgcc agcggcaacc gccaagggct gctctacgcc aaaatctcgc cgcacgacac
    93781 cgaacaaacc gaactgctgc ttgccggcta cggctacacc aaacgcctgc tcggacacat
    93841 cctgcccgaa tccgaaacct ggggcggcaa cggcatcatc cacctcaatt acagccgcac
    93901 cgaacaacaa cgcaatcacg aattgggttt gcaaaaacac cataaccacc tctaccgcag
    93961 catcacgcag gcagaagccg aaaaaatcgc cggtatcccg ctgaacacgc cctacgccga
    94021 accattatgc ggactgtttt ggcagcacgg cgtatggctc aatccacccg cattcgtccg
    94081 cgccctcctc cgccatccgc tgattggact acacgaagac acaccgttaa ccggtatttc
    94141 ccacgacgga gaaaagtgga ttgcaagcac gccaaacggc atcttttctg ccacacacat
    94201 cgtctactgc accggcgcgc acagcccctg cctgcccgaa atcaacctcg ccgccctgcc
    94261 cctcaggcaa atacgcggac aaaccggcct cacaccgtcc accccgtttt ccgaacaact
    94321 gcgttgcgcc gtttcaggcg aaagctacat cagcccgtcg tggcacggac tgcactgcta
    94381 cggcgcgagt tttattccca acagcagcca taccggatgg aacgaagccg aagaagcctc
    94441 aaaccgccaa gcattggcac accttaaccc cgcccttgcc gaatcattgt ttgccgccaa
    94501 cccaaacccc caaaaatacc aagggcacgc cgccatacgc tgcgacagcc ccgaccacct
    94561 tcccctagtc ggcgcactcg gcgacattgc cgccatgcgg cagacctaca ccaaactcgc
    94621 gctggacaaa aactaccgca tcgacacccc atgcccatac ctgcctaatg cctacgtcaa
    94681 caccgcccac ggcacacgcg ggcttgccac cgcccccatc tgcgccgccg ccgttgcagc
    94741 cgaaatccta ggcttgcccc atcccctctc aaaacgcctg cgccacgccc tacaccccaa
    94801 ccgcaccatc atccgcgcca tcgtcagaag gaaggatcta accccttaag cctttgccgc
    94861 atgccgtccc gaccacagaa aatcatattc aaataaacat accaatcctg acggtaaaat
    94921 atcgaaaaca cccataacgt tttcttccca aaataaggag taaggaatgc ctaacaaatt
    94981 gcctttgctg tccaagatga atgttccgta acattcgaag acttccttaa ccctgccagc
    95041 cgtttcggtt tctgtacccc ctgttagata aggaaggaat gaaaaagccc tgttgattgt
    95101 atgaagtaaa gggcgttaat ttttgttaat cttcccttct caggaactca atatataagg
    95161 atgttaattt ttatttccct tctcgaggac t
//
LOCUS       DAWFKN010000002        57528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFKN010000002 DAWFKN010000000
VERSION     DAWFKN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 57528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.002"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..353
                     /locus_tag="WGR46_000100"
     CDS             <1..353
                     /locus_tag="WGR46_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855133.1"
                     /translation="LAVSHHQGYKSAFAKQQAVIDKMEKDKAQALLLSAQNYARELEQ
                     ARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCIDGFGH
                     HGLQLYNRALGYGN"
     gene            250..567
                     /locus_tag="WGR46_000101"
     CDS             250..567
                     /locus_tag="WGR46_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855134.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWVNSQAAVDGSE
                     GAR"
     gene            564..892
                     /locus_tag="WGR46_000102"
                     /pseudo
     CDS             564..892
                     /locus_tag="WGR46_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            904..1170
                     /locus_tag="WGR46_000103"
     CDS             904..1170
                     /locus_tag="WGR46_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855135.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            1167..1364
                     /locus_tag="WGR46_000104"
     CDS             1167..1364
                     /locus_tag="WGR46_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855136.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            1366..1872
                     /locus_tag="WGR46_000105"
     CDS             1366..1872
                     /locus_tag="WGR46_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0855137.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            1893..3515
                     /gene="terL"
                     /locus_tag="WGR46_000106"
     CDS             1893..3515
                     /gene="terL"
                     /locus_tag="WGR46_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0855138.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVETQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            3515..5083
                     /locus_tag="WGR46_000107"
     CDS             3515..5083
                     /locus_tag="WGR46_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0855139.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSLQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTIASQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5070..6395
                     /locus_tag="WGR46_000108"
     CDS             5070..6395
                     /locus_tag="WGR46_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0855140.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            6523..6939
                     /locus_tag="WGR46_000109"
     CDS             6523..6939
                     /locus_tag="WGR46_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0855141.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            7170..8180
                     /locus_tag="WGR46_000110"
     CDS             7170..8180
                     /locus_tag="WGR46_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855142.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTSGKLLPAQKEWAEGVLKQPGGL
                     AFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESE
                     GK"
     gene            8180..9088
                     /locus_tag="WGR46_000111"
     CDS             8180..9088
                     /locus_tag="WGR46_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0855143.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            9153..9551
                     /locus_tag="WGR46_000112"
     CDS             9153..9551
                     /locus_tag="WGR46_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855144.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            9551..9973
                     /locus_tag="WGR46_000113"
     CDS             9551..9973
                     /locus_tag="WGR46_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0855145.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            9963..10613
                     /locus_tag="WGR46_000114"
     CDS             9963..10613
                     /locus_tag="WGR46_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0855146.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            10610..10807
                     /locus_tag="WGR46_000115"
     CDS             10610..10807
                     /locus_tag="WGR46_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0855147.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            10810..12219
                     /locus_tag="WGR46_000116"
     CDS             10810..12219
                     /locus_tag="WGR46_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ0855148.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            12284..12661
                     /locus_tag="WGR46_000117"
     CDS             12284..12661
                     /locus_tag="WGR46_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0855149.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            12665..13030
                     /locus_tag="WGR46_000118"
     CDS             12665..13030
                     /locus_tag="WGR46_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855150.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            12970..13239
                     /locus_tag="WGR46_000119"
     CDS             12970..13239
                     /locus_tag="WGR46_000119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855151.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(13283..13865)
                     /locus_tag="WGR46_000120"
                     /pseudo
     CDS             complement(13283..13865)
                     /locus_tag="WGR46_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14020..16191
                     /locus_tag="WGR46_000121"
     CDS             14020..16191
                     /locus_tag="WGR46_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ0855152.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            16191..17522
                     /locus_tag="WGR46_000122"
     CDS             16191..17522
                     /locus_tag="WGR46_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0855153.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            17512..18657
                     /locus_tag="WGR46_000123"
     CDS             17512..18657
                     /locus_tag="WGR46_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0855154.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            18654..18887
                     /locus_tag="WGR46_000124"
     CDS             18654..18887
                     /locus_tag="WGR46_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ0855155.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            18838..19356
                     /locus_tag="WGR46_000125"
     CDS             18838..19356
                     /locus_tag="WGR46_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0855156.1"
                     /translation="MPAIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLGTVDIA
                     ITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGIDLDDAK
                     RRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVNRIEWF
                     KAGAINVTEMPS"
     gene            19353..19914
                     /locus_tag="WGR46_000126"
                     /pseudo
     CDS             19353..19914
                     /locus_tag="WGR46_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
     gene            19925..21898
                     /locus_tag="WGR46_000127"
     CDS             19925..21898
                     /locus_tag="WGR46_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0855157.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAATQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNRNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSIVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQGLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRKSGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(22874..23176)
                     /locus_tag="WGR46_000128"
     CDS             complement(22874..23176)
                     /locus_tag="WGR46_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0855158.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(23148..23435)
                     /locus_tag="WGR46_000129"
     CDS             complement(23148..23435)
                     /locus_tag="WGR46_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0855159.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            23515..24120
                     /locus_tag="WGR46_000130"
     CDS             23515..24120
                     /locus_tag="WGR46_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855160.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            24099..24395
                     /locus_tag="WGR46_000131"
     CDS             24099..24395
                     /locus_tag="WGR46_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0855161.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKILYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            24392..24601
                     /locus_tag="WGR46_000132"
     CDS             24392..24601
                     /locus_tag="WGR46_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855162.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(24711..25952)
                     /locus_tag="WGR46_000133"
     CDS             complement(24711..25952)
                     /locus_tag="WGR46_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0855163.1"
                     /translation="MQKWQTELYSTPLWLLQTLLIVSAASAVILFLTRKTRFGREFAY
                     ILRLCLTPKNAVKVLLLITAMVTLLLTEVRLNVLSTFMSKGLYDSMQDLNASAFWIFA
                     AMNAGVVLIRAFNNVVNDFLDQGLAIKWSERLNEVLTARWLADKNYYRLQMRRHAPDN
                     IDQRIQQDAQEFIASTIEFVRGMVNSVVTSLEFAVVLRGLAGILTVFGFDIPHGIVWF
                     VYIFVILATFIAMWIGNPLIRYNYENEKLNGDYRYSLIRVRDHAESVAFYNGEKHEYD
                     QLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNGIKLFPIILQAPRLFAGQIKIGDIQQ
                     TVQAFARLQNALSFFRMFYNKFTAYRARLERLYGFLLSTEEQHSARQPEISEVSDGIA
                     LECKAAAAFARIGGSFCTAEA"
     gene            complement(26240..26887)
                     /locus_tag="WGR46_000134"
     CDS             complement(26240..26887)
                     /locus_tag="WGR46_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0855164.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(26884..27252)
                     /locus_tag="WGR46_000135"
     CDS             complement(26884..27252)
                     /locus_tag="WGR46_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0855165.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDNTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(27262..27933)
                     /locus_tag="WGR46_000136"
     CDS             complement(27262..27933)
                     /locus_tag="WGR46_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0855166.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(28098..28817)
                     /locus_tag="WGR46_000137"
     CDS             complement(28098..28817)
                     /locus_tag="WGR46_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0855167.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALLPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(28901..30214)
                     /locus_tag="WGR46_000138"
     CDS             complement(28901..30214)
                     /locus_tag="WGR46_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0855168.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(30293..31165)
                     /gene="hpnD"
                     /locus_tag="WGR46_000139"
     CDS             complement(30293..31165)
                     /gene="hpnD"
                     /locus_tag="WGR46_000139"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0855169.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            31336..33198
                     /gene="hscA"
                     /locus_tag="WGR46_000140"
     CDS             31336..33198
                     /gene="hscA"
                     /locus_tag="WGR46_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0855170.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            33280..33936
                     /locus_tag="WGR46_000141"
     CDS             33280..33936
                     /locus_tag="WGR46_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0855171.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            33972..34733
                     /locus_tag="WGR46_000142"
     CDS             33972..34733
                     /locus_tag="WGR46_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0855172.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            34788..35129
                     /gene="fdx"
                     /locus_tag="WGR46_000143"
     CDS             34788..35129
                     /gene="fdx"
                     /locus_tag="WGR46_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0855173.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(35416..36003)
                     /locus_tag="WGR46_000144"
     CDS             complement(35416..36003)
                     /locus_tag="WGR46_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0855174.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            36216..36413
                     /gene="iscX"
                     /locus_tag="WGR46_000145"
     CDS             36216..36413
                     /gene="iscX"
                     /locus_tag="WGR46_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0855175.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(36481..36882)
                     /locus_tag="WGR46_000146"
     CDS             complement(36481..36882)
                     /locus_tag="WGR46_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0855176.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            37093..38052
                     /locus_tag="WGR46_000147"
     CDS             37093..38052
                     /locus_tag="WGR46_000147"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0855177.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            38120..39430
                     /gene="tilS"
                     /locus_tag="WGR46_000148"
     CDS             38120..39430
                     /gene="tilS"
                     /locus_tag="WGR46_000148"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0855178.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            39481..40266
                     /locus_tag="WGR46_000149"
     CDS             39481..40266
                     /locus_tag="WGR46_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0855179.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            40276..40473
                     /locus_tag="WGR46_000150"
     CDS             40276..40473
                     /locus_tag="WGR46_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855180.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            40735..41088
                     /locus_tag="WGR46_000151"
     CDS             40735..41088
                     /locus_tag="WGR46_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855181.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            41100..41407
                     /locus_tag="WGR46_000152"
                     /pseudo
     CDS             41100..41407
                     /locus_tag="WGR46_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41469..42845
                     /gene="mpl"
                     /locus_tag="WGR46_000153"
     CDS             41469..42845
                     /gene="mpl"
                     /locus_tag="WGR46_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0855182.1"
                     /translation="MKHIHIIGIGGTFMGGVAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLSENVL
                     RHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            43052..44104
                     /gene="bioB"
                     /locus_tag="WGR46_000154"
     CDS             43052..44104
                     /gene="bioB"
                     /locus_tag="WGR46_000154"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0855183.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(44182..44817)
                     /locus_tag="WGR46_000155"
     CDS             complement(44182..44817)
                     /locus_tag="WGR46_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0855184.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            45140..45400
                     /locus_tag="WGR46_000156"
     CDS             45140..45400
                     /locus_tag="WGR46_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0855185.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(45463..47322)
                     /gene="ilvD"
                     /locus_tag="WGR46_000157"
     CDS             complement(45463..47322)
                     /gene="ilvD"
                     /locus_tag="WGR46_000157"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0855186.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(47495..49264)
                     /gene="cysI"
                     /locus_tag="WGR46_000158"
     CDS             complement(47495..49264)
                     /gene="cysI"
                     /locus_tag="WGR46_000158"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0855187.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(49291..51105)
                     /locus_tag="WGR46_000159"
     CDS             complement(49291..51105)
                     /locus_tag="WGR46_000159"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0855188.1"
                     /translation="MSEHDMQNTNPPLPPMPPEITQLLSGLNAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(51102..52388)
                     /locus_tag="WGR46_000160"
     CDS             complement(51102..52388)
                     /locus_tag="WGR46_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0855189.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLAIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(52427..53350)
                     /gene="cysD"
                     /locus_tag="WGR46_000161"
     CDS             complement(52427..53350)
                     /gene="cysD"
                     /locus_tag="WGR46_000161"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0855190.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(53388..54122)
                     /locus_tag="WGR46_000162"
     CDS             complement(53388..54122)
                     /locus_tag="WGR46_000162"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0855191.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(54138..55589)
                     /gene="cobA"
                     /locus_tag="WGR46_000163"
     CDS             complement(54138..55589)
                     /gene="cobA"
                     /locus_tag="WGR46_000163"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0855192.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            complement(55886..56542)
                     /locus_tag="WGR46_000164"
     CDS             complement(55886..56542)
                     /locus_tag="WGR46_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0855193.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            56607..57497
                     /locus_tag="WGR46_000165"
     CDS             56607..57497
                     /locus_tag="WGR46_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0855194.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPHVFKARAEDSHKGTFGTLAVV
                     GGAVGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT        13389 a        15099 c        16504 g        12536 t
ORIGIN      
        1 gtctggcagt gtcgcaccat caaggctaca agtcggcttt tgcgaagcag caggcggtca
       61 ttgacaagat ggaaaaagac aaagcgcaag ccctgctgtt gtcggctcaa aactacgccc
      121 gcgaactgga acaggcgcgc gcggaagcta aaaaatatga agtcaaggcg cacgccgtcg
      181 gcatggcttt ggcgaaaaaa caggcagaag tcagccgtct gaaaacggaa aataaaaagg
      241 aaatcgaaaa tgtccttact caagaccgta aaaatgcagg cggcggttgt attgacggct
      301 ttggccatca cggcttgcag ctctacaacc gcgccctcgg ctacggaaat taaggttgtc
      361 gaaaaggcgg tcatgccgac accgcctgcc gcattgatgg ttgcgccggt acgcccgaat
      421 ccgccgaaag acggcaagac ggccacgctg ttggaacacg ccgccgagtt tggcggctat
      481 gttgccgaac ttgaaaacca aaatcaggct tggcgcgact gggtcaacag tcaagcggca
      541 gttgacggtt cggagggtgc gcgatgacga cttatcgtga gttggtgcaa cgcacggtcg
      601 cctgccgcca tgcggattta gagctgggat tgagccgcgc acgcgagcaa gagccgtttg
      661 tcattcatgt ttccgatttg ttggataagg ccggtatcga ttacgcggta cgcatggata
      721 aggattttca gacgaccttt tgtgtggagt tttccgcgac gcccctgctg atgtgattgg
      781 tattttgcgg aaatattact cagtcttttc agacggccaa aaggtcgagg cggcgagtcg
      841 tcatcccgaa ggctacgcgg tccgtatcgt atttggcgat gtgccggttt aaaggggttt
      901 taaatggact ttgaatttgg tttcagaacc ctgtggccga ttgcaacggc ggcgttttgg
      961 ttttgggtca acggcatttc aggccgtctg aaagaggcgg acaagcgcat tgacgacctt
     1021 aaagaggagc tgcacgcggt caagctctct tatcacacca agcaagatgc caaggcagac
     1081 agcactaata ttgcggcagc cttagaacga attgaaaaca agttggaaaa agtaaacgaa
     1141 aaattggaca ggaaagcgga caaatcatga gcgacccgat tttggaagtc ttggcgcgta
     1201 tcgaagccaa gcaggatgac atgctcgcca atcaggctcg aatggacgag gaattgcagc
     1261 aaattaagaa agactgcaag aaatctgcgg cggtttacgg cggtctcggc ggtgtgattg
     1321 taacgaccgg ctgggagctg ctgagagcca agttcggggg ctgatatggc gcatccgaag
     1381 gaaatccgcg aaaagctgcg ccgattgtac gtcagcgacg ggcagacgct tgaaatcgcg
     1441 gcgatgatgt gcgaaatccc gattgcgacc gcccgcagct ggaaacgcgc cgccaaggaa
     1501 agcagcgacg attgggacaa agtgcgcgcc gcctatacct tggcaggcgg cggcatcgaa
     1561 gacttgagcc gttcgctgct ggcgggtttt ttggtgcagt accagtcaac gatgacaatg
     1621 ttgcaagaca cgtcgattga agggctgatg ccgtccgagc gcgccaaact gttgacaagc
     1681 ctgtccgacg cgtttaccaa gaccgtggcg gcaaacgcca aagtgatgcc ggaaacgtca
     1741 aaactggcga cggcaatcga ggtgttggaa ttgttcggcg aagtggtcag ggagcgatac
     1801 ccgcaacact tgcaggctta tgtcgagctg gtcgagccgt tgggtgtgga aattgaaaag
     1861 aaatacaggt agcaactgca taaggtaatt gaatgaagtc caaagagttt ttaaagtcac
     1921 ttgccgaata tgccgcccaa ctccgccaaa ttattgaggc agaggtggac ggcttcgatg
     1981 cgtcgcccaa ggctattgct gcacgccgtg ccaaggtgtt tgacccggta ggcggttacg
     2041 agtatttcgt caacacctac tttccgcatt atatccgctc ccctgaaaaa tccgaactgc
     2101 atgcgttttt attcagccgt ctgccggaga ttatccgctc ccccaaaggg gaaaatgagg
     2161 cggtgggtgc gccgcgtggc gagggtaagt cgacgcaggt tactcagttg tttacgctgt
     2221 ggtgtattgt gacaggccaa aaacattatg ccgttattgt gatggacagc atagatcagg
     2281 catacccgat gctcgaagcc atcaaggcgg aattggaatt taatccgcgc ttgaaaaccg
     2341 actttccgga agtatgcggg cagggccgcg tatggcaggc cggtacgatt gtgacggcca
     2401 atgacgttaa agtccaagtg gccggtagcg gtaaaaagct gcgcggtttg cgtcacggcc
     2461 cttaccgtcc tgacttaact gttttggacg atattgagaa tgacgagcaa gtccgcaacc
     2521 ccgaacagcg cgacaagctc aatgcgtggc tgactaagac tgtattgcct ttgggcggtg
     2581 tcggccagaa atacgatgtg atatatatcg gcacgatttt gcattacgac agcgtactta
     2641 accgcacttt gaataacccg ttttggcacg gtattaagtt taaggcgatg aaacgctggc
     2701 ccgaccgcat ggatttgtgg gacagatggg aggaactttt ccgaaacgac ggcgagactg
     2761 tggccgaggc gttttatctc gccaataaag acgagatgga gcgcggtgcg caaacaagct
     2821 gggcggctcg cggcgtactc gcgctgatga aaatccgtgc gcgtgacggt catgcgacat
     2881 ttgacagcga atatcaaaac gacccggtca gcggcgaaga tgcgccgttt gccaagtcga
     2941 tgaagttttg gaacgacctg ccgtccgatt tggtgtattt cggtgcgctc gacccgtcgc
     3001 tgggtaaagc gggggcgagc cgtgacccgt cggcgattat cattggcggt tatcaacgtg
     3061 aaaccggcaa actgtatgtc gtggaaactc agattaaaaa acgtctccct gatttgatta
     3121 ttgaggacgt tatcagattg caccgtcaat atcgttgcaa actgtggttt gttgagacgg
     3181 ttcagtttca ggaatttttg aaagatgagc tggtcaagcg cagcgcggcg cgtggaatac
     3241 ctgtcccggc gcgggcggtc aagccggtat cggacaagct cttgcggatt gagactttac
     3301 agcctcacat ggcgaacggt ttgattctgt tgaatgagag ccaacagacg ctgatacagc
     3361 agttccgcca ttttccaaag gctgatcatg atgatggtcc tgatgcagtg catatgctct
     3421 ggtcgggggc ggtggccaat tgtgtgccga tagaatggca aagccctacc gataacgatt
     3481 ttgatgacga gataaaaagt aaatggagcc gataatggca aaaaagaaca ataaaactaa
     3541 aatccaaaag cccgaagctg cattgcagac ggacgtggct caaattacgg cgaccggtcg
     3601 ggttatcgcc gagcatccgt ccaattttat tacaccgcaa aagatgcggg ctctcttcga
     3661 ggatgcggaa agcggcgaca tccgcgccca acacgagctt ttcgcggaca ttgaggagcg
     3721 cgacagcgac atcgcggcaa atatggggac gcgcaaacgc gcgctgctga cgctcaactg
     3781 gcgcgtcgcc ccgccgcgaa atgcgacgcc cgaagaagaa aagctgtccg accaagccta
     3841 cgaaatgatg gacagcctgc ctaccctcga agacctgatt atggatttga tggacgcggt
     3901 agggcacgga ttttctgcgt tggaggtcga gtgggtattt tcagacggcc tttacctacc
     3961 ccgaaacttt atccaccgcc cgcaaagctg gttcaaatgg gacaaagaca acgggctgct
     4021 gctgcgtacc cgcgaaaatc cggaaggcga agcgttgtgg ccgctgggct gggtcgttca
     4081 tacccaaaaa tcgcgcagcc tccagcaggc gcgcaacggg cttttccgca cgctttcctg
     4141 gctgtatatg ttcaaacact acgccgtcca cgattttgcc gagtttttgg agctgtacgg
     4201 catgcccatc cgtatcggca aatacggcgc gggcgcaacc aaagaggaaa aaaacaccct
     4261 gcttcgagcg gtggcggaaa tcggtcacaa cgcggcaggc atcatgccag aaggtatgga
     4321 aatcgagctg cacaacgctg caagcggtac gatggcaacc agcaatccgt ttttgcagat
     4381 ggccgactgg tgcgaaaaat cggcggcgcg gctgattttg gggcaaacgc taaccagcgg
     4441 tgcggacgga aaatccagca ccaacgcgct gggcaatatc cacaacgagg tacgccgcga
     4501 tttgctggtg tcggacgcaa aacaggtggc gcaaaccatc gcaagccaaa tcatcggacc
     4561 gttcctgcaa atcaactatc ccaatgccga cccaaaccgc gtgccgaaat ttgaatttga
     4621 cacgcgcgag ccgaaagaca tcgcggtctt tgccgacgct atcccgaaac tggtggatgt
     4681 cggcgtacaa atccccgaaa gctgggtgcg cgacaaactg gtcattccag atgtgcagga
     4741 gggtgaggct gtgttggtgc ggcaggtacc ggacaatccg ataaacagaa ctgcattggc
     4801 ggctttatcc gcccacaccg taccatctaa ggctacgggc aggcatcagg aaatattgga
     4861 cggcgcgttg gatgacgcgc tggttgagcc cgatttcaat tctcagctca acccgatggt
     4921 gcgtcaggcg gttgccgcac ttaatgcttg caacagctac gaggaggcag atgccgcact
     4981 gaatgcgctt tatccgaatt tggacaacgc gaaactgcgt acctatatgc agcaggcctt
     5041 gtttatcagc gatattttgg gacaagacca tgcccgcgcc tgatttggga tttgccttaa
     5101 gtctgccgcc aaaaaaggca atcgagtggc tggaaagtaa aaaggttacg gcggagagct
     5161 accgcaatct gacagcctcc gaaattgcca aagtctatac gattgcccgc atgaccgact
     5221 tggatatgct caacgacatc aaaacttcga tggttgaatc ggcaaaaagt ggacagtcgt
     5281 ttgacgattg gcgaaaaggt atcttgaatc tgctcagcaa caagggctgg ctgcatccga
     5341 acgggcataa cggtaaggat atcatcgacc cagccaccgg cgaggtattc ggttcgccgc
     5401 ggaggttgga gacgatttac cgtaccaaca tgcaaactgc ctacaacgcc ggtcaatatc
     5461 aaggatatat ggcaaatatt gatgcacgac cttattggat gtatgacgcg gtaggcgaca
     5521 gccgcacccg tccggcgcat tcggcaatag acgggctggt gtaccgctac gacgacccgt
     5581 tttgggcaac gttttacccg cccaacggct acaactgccg ttgctcggtc atcgcgctgt
     5641 cggagcggga tgtggaacgc caggggcgga ttgtcgggca aagcacgtcg gacaatcttg
     5701 ttgagaccca taaaatctac aataagaaag gcgatactta tctgaccctt gcctataaag
     5761 caccggatgg cagtctgtac acgaccgatc gaggatttga ttacaacgcc ggacgaatga
     5821 actaccgccc cgatttagac aagtacgacc gtgcgttggc gcatcaattt gccaaagcgg
     5881 aaatgggtgg tgcggatttt aaaaccagct ttaaacagct tgaaaaagag ttttatgaag
     5941 tcaagcaacg tttgaatatt gatggcaagc ccgataaaga gcagaaaatc aaaatccgaa
     6001 acgcgctatc aagacagctt aaatttgctg cgggtgtatt gagcaaggaa atgcaagaac
     6061 tggcaggtat gactcgagcg acggtgtggc tgtctgatga tacattggtc aaacaggtgg
     6121 acagtcggga ggggcaatcg tttggaacag atttatatgc catgctcccc gatctgattc
     6181 atgagcccga atatattttt tatgacaaaa gcagaggaaa agaaagtttt gttttgctcg
     6241 gaaaaccgtt ggaggggaac gaaaaataca gattgatggc agttttgaaa tatctgccac
     6301 agtacgattc gatttttttg gattcgtttc gcagggcgga tgaaaaagaa attgtgaaaa
     6361 gtaagaaacg ctatgaaatg aaaaaaggct actaagtcgg actgcaatcc cgacacactc
     6421 tcgcttatgt aacggatacc aatcccagtc cgaggactgc ataagggctg cggagggcag
     6481 acaaattcac cgctttttta gtagcctagg caggataata ccatgattga tgtcaaaata
     6541 gacaatatct ttgtcgtcct aaaccaaatc gagcggcttg gcaacgggat cgaaaaccgc
     6601 tacctgctga tgcgccgact gtccgaaacc atgcacacgg cggtcaagct caatttccgc
     6661 tacgcaggcc gtccgaaatg gttggggcta aaataccgcg acggcaagcc gctttcggat
     6721 tcgggtcgtc tgaaagacag tttttccaca ctgtcagaca acgatacagc ccttgtcggt
     6781 acgaatatcg tctatgccgc catccacaac ttcggcggta tggcggggcg caaccgcaaa
     6841 gttcggattc cgcaacggga atttttgacg ctgacggacg acgacaaaca ggctttgatg
     6901 gacgatgtgc aggattattt ttcgggtctg ataccgtgaa tttataaaac cctcaaaaac
     6961 gcgcttttta gcgcgttttt ttatgcgggt aatacaaacc cctgcccaag atataaaaat
     7021 caatcctaga cgcttctaaa aagcccctga aaacgattaa ttgtgtatcg cgcggacagg
     7081 ttttaaaaaa atggcgggag ggtttgaagc acgcctactc tttgttgttt tttcaaatag
     7141 gcaaaatgac cgtattgaga gaggtacaca tgtccaaaaa tgcacaaaaa accctacttg
     7201 ccgtgtgcag tttcgaggtg cagccaaaag acgggcgaat ccaactgctg ccatatggcg
     7261 aatttcgcgc agtagacggt cgtccgactg atgtccctgc gtggtatctg accgaagaaa
     7321 acggtcatga tgtcgcgttg ttggccaaca gctcgcgcaa tcagttggtt gtcgattatg
     7381 aacaccagac gctctacaaa gagaaaaacg gacaacctgc acctgccgcc ggttggatgc
     7441 gttggctgga gttcacgcct aaaggcatgt ttgccgaagt ggagtggacg gacaaggcgg
     7501 ctgcggcaat tgccgcaaaa gagtatcgct acatctctgc tgtgttttcc tatgacacaa
     7561 agggatatgt aagcaaaatt tttcacgccg cgctgacaaa tttccccgcg ttggacggta
     7621 tggacgaggt gctggcggca gcgtcggcgc aaattttaaa accggaaacg gagcaaaacc
     7681 ctatgaaaga gttgttacag caactgttcg gtctgcctga tgcgggcgaa gaagaactga
     7741 aggcggcatt gtccgcgctc gtggaagcca agccgaaaga cgtggcattg tctgccgacg
     7801 tgttcgcgca gctggcggaa aaagacagcc gcatcgcggc attgacggcg caaaccgcca
     7861 agcctgattt gactaaatac gcgcctatct cagtggttca agagctgcaa agcaaagtcg
     7921 ccgcgctgac ttcaggcaaa ttgctgcctg ctcagaagga gtgggcagaa ggcgtattga
     7981 aacagccggg cggcttggca tttttgaccg gctttattga aaacgcccag ccggtcgctg
     8041 cactggcagg ctcgcaaacg ggcggtaaag cacccgacga acgcgtcgcc gcactgactg
     8101 cggaagaggc agccgcagca aaaatgctgg gcatgtccgg cgaagaattt gtaaaaatca
     8161 aagaaagcga aggtaagtaa tggacaaatc agcgattttg accgcaatca cggcagcatt
     8221 ccgcaaagaa tttcaaacgg gtttggaatc cgtcaagccc gactataccg ccgttgcgat
     8281 gaccattccg tccaatacgg caaccaatac ttatgcgtgg ctcggcaaat tcccccaaat
     8341 gcgcgaatgg gtcggctcgc gccaaatcga aaaaatgaac aagcaggcga tgagcctcga
     8401 caacaaaaag tttgaagcaa ccgtcggcgt agcgcgtacg gacatcgaag acgaccaagt
     8461 cggcatgtac cgcccgatga tggcggcaat gggcgagtcc gccgccgccc tgcccgatac
     8521 gctggtgtgg ggtctgctca aaaaaggtaa aaccactgtc ggttatgacg gccaatattt
     8581 cttcgatacc gaccaccccg tttacgagaa atcggacggc acgggtcaga atacgccgca
     8641 ctccaatctg acgaccggta cggacaacga cgcgccgacc ttctacgtcg tcgatgacac
     8701 caagaccctg aagccgctga ttttccaaaa ccgcaccgac tccgagtttg aaaccaaatt
     8761 cgacccgtcc aaatccgacc gcgtgtttat ggaagacgaa tacctgtacg gatcgcgccg
     8821 ccgctgcaat gccggtttcg gtttgtggca actgatgcac atggcggaaa aaaccgcgct
     8881 gacccgcgaa aacctcgccg ccattatcgt caaaatgcag aaaatcaaag ccgacggtgg
     8941 ctatgtgttg aatgtgaagc ccagcctctt ggtcgttccg cccgagctgg aagacaaagc
     9001 ccgtgaatta ttggaagccg acaaaatcaa cggcacgacc aataccttca aaggccgtct
     9061 gaaactgcac gtttgcgtcc acctgtaacc ccgaaccgtt ttcagacgac ctttaacacc
     9121 tatttaaagg ccgtctgaaa caaggagtca tcatggcaaa aaccaacaac aaaccagaaa
     9181 ccgccgaaac cgccgccccg tcgtttgaag acatcaaagc cgaattggac gccgtgcagg
     9241 cagagcttgc cgccgcccga aacgatgtcg aaatgctgac cacagccttg gaaaaagccg
     9301 aagacgacaa aaaggcactg tccgccgaac ttgccgaact caaagtgcag catacgcaac
     9361 gcgccgccga cgctttggcg gacagccgcg atgtgatgct cgtcagtacc ggcgcagacg
     9421 gcaaagaatt ttggcgcggc ggcctgctgt ttgacggcgg ctggcgtgaa gtgaagcgcg
     9481 ccgaagtcgg cgaagcggtg tggaaggcaa tctgcgccga gcctatgctg caacgcaagg
     9541 cggtcgagta atggcatacg cgacggttga ggatatggtt gcgcgtttcg gtgagctgga
     9601 agtcttgcag ctcaccgacc gcaacaacga ggggctgatt gaccgcgagg tcgcacaaac
     9661 cgcgctggtg gacgccactg ccgaaatcga cgcgtatctg gggcggttca gacgaccttt
     9721 tgaggatctg ccgcccatct tggtgcgcct ttgctgcgac attgcccgct accgtctgac
     9781 ggcggctcag ggcgtgttga ttaccgacga aatccgcaac cgctacaaaa tcgacgtgct
     9841 cgacctgctg cgtgctatgg ccaaaggcga agtgcagctg ggcgtggatg atagcggcga
     9901 agaagtggcc gcgggcgaag acggtattgt gtttgtaaac ggtaaaaata aggtgttcgg
     9961 gcgtgatcac tgatattgag caagcgataa cagaccgtct gaaacggggc ttgggtcgca
    10021 tggtgcgcac ggttaaaagc tacaacggcg aggccgacga tttggcgggg caaatccata
    10081 cgctgcctgc ggtttgggta acgtatggcg gcagcaaagt tgagcctgcc agcaccggcg
    10141 gcgtatgcgg acgttatcag gataccgccg aatttgtggt gatggtggcg gcccgcaatc
    10201 tgcgcaacga gcaggcgcag cggcaaggcg gcatcgacag ccgcgaaatc ggcagcaacg
    10261 atttaatccg cgctgttcgc cgcctacttg acggccagcg gctcggtttt gccgatagcc
    10321 gcggcttggt gcccaaagcg gtgcgcgcga ttgccaatca tgtgctggtg caaaacgccg
    10381 cagtaagcat atatgcggtt gagtatgcca tccgctttaa cacctgcggg ttggaaaatg
    10441 accgctaccc cgaacgcacc gacaatcccg acgaccccaa ccatatcttt accaagtatc
    10501 agggtacatt gagcgagccg tggcctgatt tcgaggggtt ggacggcaaa atttacgacc
    10561 cgcaatccgc cgatgaaata cctgtaaacc taacccttaa ggataagcaa tgagcaaaat
    10621 caaagtaacg gcggcagatg gcctgcgtgt gccgaccgaa cacaacccgc acgaatatat
    10681 cggccaagag ccggtggagg tggacggcaa cagcctgtat taccgccgca tgattgatga
    10741 cggcgatttg gtggtggttg aggatgccgc cccaaatacc aaaacccgca atactaaggg
    10801 agagtaatga tgccccatat tgattttgac acgattccgg gcagcatccg cgtgcccggg
    10861 cagtatattg aatttaacac ccgcaatgcc gtgcaaggtt tgccgcaaaa tccgcaaaag
    10921 gtattgatgg ttgcacccat gctgaccgcg ggcatacagc ccgccttaga gccggtgcaa
    10981 ctatttagcg atgccgaggc ggccgatttg ttcggacaag gctcgctggc gcatttgatg
    11041 gtgcgccaag catttgccaa caacccttat ttggatttga ccgttatcgg tattgccgac
    11101 cacagcgcag gcgtgcaggc aaccgcaacc gttacccttt ccggcacggc caccgcgccg
    11161 ggcgtggtgg aaatcacgat tggcggcaag caggtaagca cggccgttaa caccggcgag
    11221 accgccgcca cagtggcagg ccgtctgaaa accgccatca ctgccgccga tgtaaccgtt
    11281 accgcatccg gcagcggcgc agccgttacg ctgacggcca aacacaaagg cgagatcggc
    11341 aacgagagcg gcttaaccgt gagcaccggc aataccggcc tgacttatca agccaatgtc
    11401 tttaccggcg gtgccaaaaa tgcggacatt gccacggcct tgtccaaagt ggcgggcaag
    11461 cattatcaca ttatttgcag cccgtttagc gatgacgcca acgccaaagc cttgagcaac
    11521 catattacca acgtattcaa cgccatcgag cagcgcggct gtatcggcgt attgggtatg
    11581 agtgcggcct tgagcacggc caccaccgct accggcaaaa tcaacgacgg ccgcattacc
    11641 tgtgcttggt acaaaggtgc ggtagagcca aacggcatca tcgccgcagg ttatgcggcg
    11701 gtgttggcct ttgaagaaga ccctgccaag ccgctgaaca cgctggaaat caaagggctg
    11761 gccgttacac ctgatgcgca atggccgtgg tttgcagaat gcaacaatgc gctgtacaac
    11821 ggcttgaccc cgctcacagc ggtcaacaac cgcgtgcaga ttatgcgtgc cgtatcaacc
    11881 tataccaagt cggccaacaa caccgacgac ccggcactac tcgacattac caccattcgc
    11941 acgctggatt atgtgcgccg cagcgttaaa gagcgcattg ccctgcgttt tccgcgcgac
    12001 aaattgagcg accgcctgct gcccaaggtt aagagcgaga ttttggacgt gctgattaag
    12061 ctcgaccaag ccgaaatcat cgaaaacgcc gaggccaaca aaggcaagct ggtggtggcg
    12121 cgtgcgcaaa acgaccccaa ccgtgttaat gccattatcc ccgccgatgt ggtcaacggc
    12181 ctgcacgtct ttgccgggcg cattgatttg attttgtaac ccttttcaga cggcgtttaa
    12241 aacaggttta aaggccgtct gaaaccttaa aaaaggataa agcatgagcg acgctaccta
    12301 taccggcgcg gtgattatgg aggtgaacgg ccgcgatatc gagattgtga gcatcaaacc
    12361 gcaaaccact acaggccgca agccggtcaa aacgatgaac cgcaacggcc gagtcaacgg
    12421 ttattgtgac ggcgtaaccg aacacaaatt aagcgttacc gccgccattc cgatcgacgg
    12481 tacggaaatc gactgggaca acatcaccaa ggcgaaaatc acgatttacc ccatcaacga
    12541 cgaaggtcgc cgcacttcct acctcgactg ctttaccgtc gataccagcg agcaatatga
    12601 agtcgataac gaggcacgca tcgacattga gatgattgct ttgcacaaaa tcaaggagta
    12661 atgcgtgatt accgtcaaac tgacccacgg gctgacctac aatggcaaag tcgtatctga
    12721 attacgcctc aagccactga ccgtcggcgg cgaactggcg gcgttcgccc tgattgatga
    12781 cttgcccgag ctgcccgaaa acgccacaaa agccgaactg ctgcaacgcg acgtcctaga
    12841 gacgctgacc tactggtcgc agcagattga agcccaaggt atcccatccg acatcctgac
    12901 ggcgcagtgg ctgatggaaa acctctctac cgaagactac cataccgtga tggcggctca
    12961 ggaggatttg tgcctaaaac cgtccgccgc tacggcgagc cccgacgcgc cgtcggcggc
    13021 ggagcagtaa aacgcagcta cctgacggca cacaaaagct accgtcaggc ggtcatcctg
    13081 atggcaaagg cggggatagg ggcggacgca gtgcgcgata tgtgccacgc cgagctgtcg
    13141 gcatggtttg aagacatcct gtcgagcctg ggcataaaaa cgccgaagga agagggagtg
    13201 attgtgtcga aacggctgag aaagccggag ggaaaataaa aaggtcgtct gagtttcaga
    13261 cgaccttttt tttatttgac ggttagggtt gttttctgcc gattattgca atggtgtttg
    13321 tttctttttc aaaaacaacg ctatataaaa taccatctgc cggaacgtct ttttgcgctg
    13381 ttgctcctgt ctgattggat tctttgacga agttgttttc ctgcttccga ttcgcccaat
    13441 ttgtctgtgg tagttaaaga cagaactgcc tgaatactgt gtaatccatt tatggcatta
    13501 ttgactagat tttgtccttt ctctccgtcg gtttttaaaa tgtagcctac tgagatagct
    13561 ctaatttttt gctgctcgtc cagtttgagc gcaatcgccc caaaatccat taccaaagta
    13621 aggtcataac cacaatcggt cttgacaata tttttattat ggatttttgt tgagacgttc
    13681 tgagttttaa atgcttgctc caaattgatg agatattgtt caactgtgat gtccattggg
    13741 gcggtacaat cagcagcctg gatactttgg acaggttctt cggcttgtgc cgtttttgat
    13801 tgttggttgc aggctgataa aacaattgaa acacaaattg cggaaatcaa ttttttcata
    13861 ttcataaaat atccctttga ataaaatggt tatcattctg gtattataac gcaacaaaca
    13921 aataaagcac gaaaacgggg ttgaagccca taccgcctcc cttaaacagc ctttaaacga
    13981 taattgacct tgagttaata cgtttaaagg ctgcttttta tggcaaacgg gaacatgaaa
    14041 ctgtcgttgg tgttaaccgc ccgagatgac ggagcgagac ggctactggc tgatactcaa
    14101 cgacaattag atcgtaccgc gaaatcgcgg gcgcaacttg aacggcaaag ccatacttat
    14161 gcgttgaccg gcatccgctc agaaaaacag attcaacgcg aaatcatgct gacacaggct
    14221 gcgtttaacc gtttggcgcg cagcggcaag gcatcacaaa atgatttggc acgggcggcg
    14281 gtcgctacgc gtaaccgaat tcgcgagctg aacgcggaac tgaaacaggg cacgggattt
    14341 gcggacaaga tgggaaaaat cggaagattc ggtgcagctg cggtggctgg tggcgcggca
    14401 gcgtatacgg tgcttaagcc tgctatggac aacagaaagc agcttgatga gaacatcaac
    14461 cgcgtgtcca gacaggcatt tattgaggat aacagtaaat cggcagcgtg gattgcaact
    14521 gaaggtgcgc aacagatcaa ggatttggca cttgaacttg tcgagaaaaa tggcgggacc
    14581 cacgataagg ctttggattt aatcagcggc atgatgacca ccagtctgaa ttttgcccaa
    14641 accaagaatg aagcgcaggc ggcatatgct tttgcacttg cctcagaagg cagtggcgag
    14701 gatacggcaa aactgattaa aaccctgaaa gatggcggca tgagcggtaa agacctgcaa
    14761 ctcgggcttg agcacgtctt gcaatcgggt ttagacggca ctttcgaggt gcgggatatg
    14821 gttcgggagc tgccgagtct gctccctgcc gcgcaacagg cagggatgaa tggtgtcggc
    14881 ggtttggact acctgctctc actcttacaa tctgcggcga ataaatcggg cagtcctgcc
    14941 gaagcggcga ctaatgtgca aaatcttttg agtaaaactc tgtcgcctta cacgataggt
    15001 cgtctgaaga agatggcaaa tccaaatgac ccgaagaaag gtgtcgattg gataggctcg
    15061 gttgtgcaag gcaagcaaaa cggcgaaaac gcagtgcagg tgttgtcccg tcttgccgat
    15121 gccatgctag taaaggataa gcaataccaa gattataaga aacgcgcggc tgcaggcgat
    15181 aagacggcgg cggagcaggc aaatatgctt aagggcgcgc ttttggcgca actgctgcct
    15241 gatttgcagg caaaacaagg tttgctggct gcaacggata tgacgcaaat ccgtgaatat
    15301 atggcttcgt tggctggcgt aacgttggat aacggaaaaa ttgctaagaa caacgaggcg
    15361 cgaatgttgt cggcagcggc gcaacaagag caacaggaat cgctggcaat gttgcgggaa
    15421 agtctgacgg gaacattggt ggatatggaa acctcgttta aaaagctggc agcggaatac
    15481 cctaatgcca ctctagccct gcaagcattg acgacggcgg caacagcggc gtctgccgca
    15541 atgttattaa ccgccggtgg cggtaaaggt gcaggctttc tgaaagatgt aggtagtaaa
    15601 gcgttggtat ggggtaaggc ttccgcaggc ggcgtggcag caggtgccac agcggcaggc
    15661 ggtaagttgc tgtcatgggg aaaatctgcc ggtagcgggc tcatgaataa tccagcgtta
    15721 gttaaacggg cgggtttgtt aggtatgttg ctgtattccg agtctttggg tgacggcaca
    15781 ttgccaaagg gtttgcgtgg taccaagaca actcctgaaa tgattaatcg tctgaaaaac
    15841 aacggtatcc gatttgaacc tgcgccgaag cgggaacagg cgcggggtgg tgtccctcag
    15901 tatttggctg ctccgtcagc gcagcctacc gataagatgt tgtctccgtt gttttcaact
    15961 cagacggcgg cgtatcaggc agccattcag cagcagacgg cggcgtatca ggcagcattg
    16021 gcgcaggata cggctgcagt tacaacaggt ttggcacaag tgcaaagtgc gatggcgtcg
    16081 gcaagtcaga ccatcaatac caatgtgagc ctgaatatcg acggacgtgt tatcgcgaat
    16141 gaggtatcgc ggtatcaagt ggccatgttc ggccgtggag cgggtcaata atgagcggat
    16201 ggcatacctt attgcaggac gcatcttaca agggcgtcgg ctttgatatt gaggtggtgg
    16261 acgagagcaa cggcaaggca ttggccgagc atgcgcggcc gtttgtgcag ggtatcgacc
    16321 ttgaagacat gggcatgacc gggcggcagg tgcagattaa tgcggtgttt tggggcaagg
    16381 gctatgcagg ccgtctgaaa aagctgctgg atgcgctgga gcagccgggc ggcggcgtgc
    16441 tggtgcaccc tgtttggggg cggatgcaca acatgattgc ggcatcatgg agttaccgac
    16501 atgaggccga ttatgtggat tatgcgggca tcgatattac tttccgcgag gcggccgaag
    16561 cgcaggaaat ctttgttttt gaaaacgcct ttttggtcga gcttgaggcg ttgattgctg
    16621 atatcgacac ctaccgcgag gcggctatcg gctttgttga tgcggtgttg gcggtggatg
    16681 cgggcgtatc ggctttatgg ggcagcgcgc tgggcatttg gagtgcggca tcgggtacgt
    16741 ttggtgcggt gcgccgtttg tttgatttgg acaaaattgc ctttcccgat cggggcggat
    16801 acagcgcagc ggcgtttaaa aacggctcgg ccaagctgtt tgcggatata tcggtcatgg
    16861 tagatactgg catacgccgt gaggcgggtt tggccgataa tgccatgcac catgccggtt
    16921 ggtcgccgcg acagcggttt gacggggctg cggctgttgc cgaccgtgcc gccgctatcc
    16981 ctgataattt gctgaccggc cgcttttcag acggcctgca aaaccgcctg aaccgtttaa
    17041 ccgccaaaca ggtgcagccg gtagcgcagg cggtgcgcct gttatccacg tcatcgctgt
    17101 tgtcggtggc aacggcatta atcgaggcgc atggcgaaga gatgaccgcg cccgatttga
    17161 ttgaggttaa ccgcgccatg cgccgccgta tgcaggccga gattgccgcc ttacgggcgg
    17221 tgcagacggc tgctgccgag tctggtgggc tgacggccaa cgccgtgtat accgaggctt
    17281 accaaacggc agaatccctg cgcgcggcgg caggccgtct gaatgcgttg gttgcggcgg
    17341 tcatcaacca aaagccgccg ctgattgtgc gccaagcccc aatcgacggt acgatacacc
    17401 aaatcgccca cgagttttac ggcgatatag cccgcgcagc agagctggtg cggctcaatc
    17461 cccatatcca ccaccccgcg tttatcaagc gcggcacttt ggtcaacagc tatgcaaaat
    17521 aattcatacg gctatgccgt gtcggtgcgc gtgggcggta aagagcaccg ccactgggag
    17581 cgctacgaca tcgacagcga ctttttaatc cctgccgaca gcttcgattt tgtcatcggc
    17641 aggttggggc cggaggcggc catacccgat ttaagcggag agagctgcga ggtagtgata
    17701 gacgggcaaa tcgtgatgac gggcatcatc ggcagccagc gccacggcaa aagcaagggc
    17761 ggccgcgagt tgagcttgag cgggcgtgat ttggccggtt ttttggtgga ttgctccgcg
    17821 ccgcagctca atgtaaaggg catgacggta ttggatgcag ccaaaaagct ggccgcgccg
    17881 tggccgcaga ttaaagcggt ggtgcttaag gccgaaaaca accccgcttt ggacaaaatc
    17941 gacatcgagc cgggcgaaac cgtatggcag gcattaaccc atattgccaa ctcggtcggg
    18001 ctgcatccgt ggctggagcc ggacggcacg ttggtggtgg gcggtgcgga ttacagcagc
    18061 ccgccggtgg cgacattgtg ttggagccgc accgacagcc gccgcaatat cgagcgcatg
    18121 gacattgagt gggataccga caaccgcttt tccgaggtta cttttttggc gcaatcgcac
    18181 ggccgcagcg gcgacagcgc caaacacgat ttaaagtggg tgtacaaaga cccgacgatg
    18241 acgctgcacc gccctaaaac ggtggtggtg tccgatgccg acaatttggc cgcattgcaa
    18301 aagcaggcta aaaagcagct ggccgactgg cggctggagg gattgacact cacgataacc
    18361 gtgggcggcc ataaaacccg cgacggcgta ttgtggcaac ctggccagcg tgtgcatgtg
    18421 atcgacgacg agcacggtat cgatgcggtg ttttttctga tggggcggcg gtttatgcta
    18481 tcccgcatgg atggcacgca aaccgagctg cggctcaaag aggacggtat ttggacaccc
    18541 gacgcttacc ccaaaaaggc cgaggcggcg cgcaagcgca aaggcaaacg caaaggcgtg
    18601 agccataagg gcaaaaaagg cggcaaaaaa caagcagaaa cggcggtgtt tgaatgagtt
    18661 tgagtaaatt ggcgaaaaaa acggcacaaa ctgctaaaaa tatcggcgaa accctgcgcg
    18721 cggcctttcg gggaaaaatc acgctggtgg tgtcgtccga gccgatacag cgcgtgcagt
    18781 tgagcggctt ggccgacgaa accctgcaag accttgaaca tttgcaggaa tacggctttg
    18841 ccagccatcc gccgaccgcc cgcaggcggc aaccgttacg actataaaaa ctgggcgttg
    18901 agtgttgacg gcgtaaccag cgcatatgtt tatccgctgc gccgcggctt gggtacggtg
    18961 gatattgcca ttacctccgc cgacggtgtg ccgtcggaag aaactgtgcg ccgcgtacag
    19021 gcttatatcg acgagatgcg cccggtaacg gcaaaaaatg cgctggtact caagccaacc
    19081 gtaacggcgg tgcctgttac cgtgcaagtc aagctcgacg gcatcgactt ggacgatgcc
    19141 aagcgccgca tacggacggc cctaaaagaa tatttcgaca ccctgatccc cggcgacggc
    19201 ctgactgtgt cgcaaatcga ggcggctatc agcaatgtgg atggtgtgat cgaccgccgt
    19261 ctgactgcgc cgacggccaa ccgtgccgcc gatacggtta accgcatcga gtggtttaaa
    19321 gcgggcgcga ttaatgtaac ggagatgccg tcatgagcta tcaagacatc ttgcggggtc
    19381 tgttgccccc ccgtgtcgta tgcccgcaat gccccgcgtg tgcgggcgca ggcagaaata
    19441 gacggcgcag cgctggatgc ggtagcggaa tcggctcaaa gcgttgccga tgccgtcgac
    19501 ccgcgcagcg ccggccaaat gctggccgat tgggagcgcg tattaggttt ggacggtacg
    19561 ggcaaaaacc gccagcgccg tgtgttggcc gtcatggcca agctaaacga aacaggcggc
    19621 ttgagtattc cttattttgt gcgtttggcc gaggcggcgg gctatcaaat ccaaatcgac
    19681 gaaccgcagc cgttccgcgc cggtgtcaac cgtgcgggcg accgtcttgc gccgcaggaa
    19741 atcatgtggg tgtggcacgt taacgtgcgc ggcggcaaca accgcattac ccgattccgc
    19801 gccggtatct cggcggcggg cgacaggctg accgattaca gcgatgccgt gatcgaaagc
    19861 ctgttcaacc gcctcaagcc cgcccacacc gctatccgat ttacctaccg ataaggacta
    19921 aaccatgcac cccatcgaaa cccccgataa gaccttccac gacggcgacg gcgtgtccga
    19981 attgggcacc atcctgcccg cgtggtggct caaccaagtg caatccgagc tgctggccgt
    20041 gctgactgcg gccggtatcc agccggataa gtcgcagcct aatcaattac tggcagcact
    20101 gaatagactg gctgtagtta ccaccggcaa ccaagaaatc ggcggccaaa aaacctttac
    20161 cgccgcaacc caattccaaa gcggcatcca tttatccgcc aaccagacga actggaacgg
    20221 cggccacaaa gcctacatcg gcgcggatgc cgacaacgcc cacatcgtct tcggcgacga
    20281 caccctccgc ctgcacagcg caaacaaccg catttcctac aacaaccacg acatcttcca
    20341 caaagccaac aaaccgcgtt ttgccgaaga catcgaaggc aaaccgaaca cactgtccgg
    20401 atacggcatc ggcaatttca aagtcgaaac attccggggc gatttgaaca ccctcaaaac
    20461 agacggcatc tattccctgc cgacagcggg cggcagctcc aacctgcccg ttgaaaacac
    20521 tgcctgccat atccaagtca tcgccggcac gaaacacggc tggtgcaggc agttgggtta
    20581 tcccgcctac acgtccgacg tgtacgaacg ccaccaaacg agcagcgcaa acgacaactg
    20641 gtccgcatgg aaaaaactca attcggacgg catccccgtc ggcgcgatcg tatcctttcc
    20701 caaagccgtc cgaaaccccg caggctatct caaagccaac ggcacgacct ttgcgcaaaa
    20761 caccttcccc gacctctacc gcgccttggg caaccgcaac cgcctgcccg atttgagccg
    20821 taccgacatc ggcatcaccg cgtggtttcc gtccgaccaa atcccgaccg gctggctggc
    20881 gttcgacgac atccgcacgc gcgtaaccga aaccgcttat cccgagctgt accgcctgct
    20941 gaccggaaaa tacggcagca tccaaaacgt cccgcaggcg gaagaccgct ttatccgcaa
    21001 cgccgccaac ggcttggcag tcggaacgaa gcaggaagac gaaatcaaac ggcacgtcca
    21061 caaagtattt tcacactggg taaaccaccc gcacgcggca gccctcggtt acgaagacca
    21121 caacgaaagg cagagaagct cgatcgtatc gacttggacg gacgaaaatt taagcgacaa
    21181 cggcttttta accccgcgcc cggacagcaa aatggcaacg ggcggcgacg aaaaccgccc
    21241 caaagccctg gttttaaaac tgtgcatcaa agccgccgac accttgggcg aagccgtgtt
    21301 ttggataaag tcccacggcg aaaccgtcaa cgccggcgcg ctggacgcgg gcacgctggc
    21361 gcaaggtttg caagacaaag ccgaccgcgc ccacacccac accgccgccc aaatccaagg
    21421 gctggacgaa aaaatcagca ccgccgttgc cgcgcaattc acacgccaaa ccatcggcgg
    21481 cgtggatatt gtcagattcc ccgacggcac aatgatacag accggcagct acaggttcac
    21541 acgaagcggc ggccccatcg aaaacgaagt cgtcttcccc gtcgcctttg ccgacggcaa
    21601 cgtcaaatgc ttcgtatccg aacgccattc gggacgcgtt accggcgatc gaaggcaaca
    21661 caactggctg tttatccgcg caaaaaacca cgccgccgcc attattacca actggtacga
    21721 aggcagttgc gactggatgg ccatcggcaa agccgcctcg ggaaacgccg ccagctcccc
    21781 gataggcccc gaaatacctg aaaccaacga agaaccgcaa agaaagagtg gaagaacatc
    21841 aaccggaccc cgaaaccgcc gccgccgaga cggcttgctc gaggcactgc aagactaggg
    21901 ggctgtagag atggctgtag agacgggctg tagagatggc tgtagagacg ggctgtagag
    21961 atggctgtag agacgggctg tagagatggc tgtagagacg ggctgtagag acgggctgta
    22021 gagatggctg tagagacggg ctgtagagat gggctgtaga gatgggctgt agagatgggc
    22081 tgtagagatg ggcttcagcc cgccgatcca agcaatccga ccgaaaccgg ccgcgccgcc
    22141 aatcccccga aacctatgcc ccgccaatcc tgccactctt cgtcattccc gccgctttcg
    22201 tcattcccgc gaaagcggga atccagcccc taaacgcggc aggaatctat cggaaaaaac
    22261 agaaaccccc gccaccgtca ttcccgcgaa agcgggaatc tagaccccaa acgcggcagg
    22321 aatctatcgg aaaaaaccga ccccccgcca ccgtcattcc cgcgaaagcg ggaatccaga
    22381 ccccaaacgc ggcaggaatc tatcggaaaa aacagaaacc cccgccgccg tcattcccgc
    22441 gcaggcggga atccagaccc caaactcggc aggaatctat cggaaaaaac agaaaccccc
    22501 gccgccgtca ttcccgcgca ggcgggaatc cagaccccaa actcggcagg aatttatcgg
    22561 aaaaaacaga aacccccgcc gccgtcattc ccgcgaaagc gggaatccag accccaaacg
    22621 cggcaggaat ctatcggaaa cggctgaaac cgaacggact ggattcccgc tttcgcggga
    22681 atgacggcgg caggggtttc gggattcccg ccttcgcggg aatgacggaa agtggcggga
    22741 ataacgaaag gcgggaatga ccgcgcaaaa agccgctgcc cccttcggac ggcaccggca
    22801 acaaaaaacc gcacggccga aaccgcgcgg gaaaggatag tcgggcgcgc ccgataagca
    22861 gcggccgccc ctgttatttc aattgggcga catattggcg caaaacctcg ttgatgcgcg
    22921 tctgccagcc cttgccgccg gcgcggaatt tttcgaccac atcggcggac aggcgtatgg
    22981 taacgagttg tttcggggtt ttgcctgtgt ttcgtttttg cattacgccc ttttcttcca
    23041 attgtttttg atgggaaaaa agcacctgtg ccaagtcttc gggcagggct tcggcaatgg
    23101 ggcgggcaag cgcaaaatct tcggcggcaa gttcccgcac ttcgccgtca gcgtttgtta
    23161 aggattgacg ttgcatattt tttaacctct cttttattcg ctttgcgaaa actgatgaca
    23221 cggatgccgt cttttatcgg cgtaaaacag acaatgtgca ggcgttgcgt atcgcctaga
    23281 taagcagcgg caacataacg cggttcgggg taatcaaagc ggacatcggg cacaataacg
    23341 gccgttgtcc agcgtatttg cccgactgat tcaaagggca aattccgctc ttcgatattg
    23401 cgttgatttt tttcggagtc aaattcaatc ttcattgcag cttgcagcgt attttgtcgt
    23461 tacattataa gcggcaaaaa accaaaatgt aaatacaaaa aaggaaaccc cgaaatgacc
    23521 atctatttca aaaacggctt ttacgacgac acattgggcg gcatccccga aggcgcggtt
    23581 gccgtccgcg ccgaagaata cgccgccctt ttggcaggac aggcgcaggg agggcagatt
    23641 gccgcagatt ccgacggtcg ccccgtttta accccgccgc gcccgtctga ataccacgaa
    23701 tgggacggca agaaatggga aatcggcgaa gccgctgccg ccgcccgttt cgccgaacaa
    23761 aaaaccgcca cggcattccg cctcgcggca aaggcggacg aactcaaaaa cagcctcttg
    23821 gcgggctatc cccaagtgga aatcgacagc ttttacaggc aggaaaaaga agccctcgcg
    23881 cggcaggcgg acaacaacgc cccgaccccg atgctggcgc aaatcgccgc cacaaggggc
    23941 gtggaattgg acgttttgat tgaaaaagtt atcgaaaaat ccgcccgcct ggctgttgcc
    24001 gccggcgcga ttatcggaaa gcgtcagcag ctcgaagaca aattgaacac catcgaaacc
    24061 gcgcccggat tggacgcgct ggaaaaggaa atcgaagaat ggacgctaaa catcggctga
    24121 aaaaatacgt ttaccacctg ttggtagcca tcgaccaact gttcaacgcc ctaaccggcg
    24181 gcgcggcgga cgaaaccctc tcaagccgca cctatcgcgg cgcgcggctc gcccaaaagc
    24241 ccaaaacccg ctggaagatt ttatataccc tgattaacgg cgtgtttttc gaccgccaac
    24301 actgtcggca ggcgtatatc agcgaactga aaggcaggca gcacgacgcg cggttcaacc
    24361 aaagccgcgc cgccggggaa aaggggacgc gatgaactac ttcggcatgg tagagtttct
    24421 gcgcctgatg gcaatggtgc ggccgccatt tgtcttcttc tccggcaccc gcagcgaact
    24481 gcctgcttat cttgacctcg tggcagaact gcgcctgacc ggatgggagc gttttgcagg
    24541 cagccaaacc ttgactgtga gcagcacatc aacagcaatt caagctacga cgaccacctg
    24601 atttataagt tctgaccgca agtagcgtac tacttttaaa ggcataagat aatccccgtt
    24661 taacagacca ttaaacgggg ataaatttgt gcaaaagcta atacaatttc ctacgcttcg
    24721 gcggtgcaaa agctgccgcc aattcgtgca aaagctgccg ccgccttaca ttccagtgca
    24781 atgccgtctg aaacttcgct aatctcgggt tgtcgcgcgc tgtgttgttc ttcggtactc
    24841 agcaaaaagc cgtacagacg ctccaatcgg gcgcggtagg cggtgaattt gttgtagaac
    24901 attcgaaaga aagaaagcgc gttttgcagt cgcgcgaagg cttggacggt ttgctggatg
    24961 tcgccgattt tgatttgtcc ggcaaacagg cgcggagctt gcaaaataat cgggaagagt
    25021 ttgatgccgt tggtgaacat atcgttaaag ccgcttaagc agacgctttg ccgcgcaatg
    25081 cgccaacggt tgcggataat ggctttaaag cggtcggaaa gctggtcgta ttcatgtttt
    25141 tcgccgttgt aaaacgccac gctttcggcg tggtcgcgca cgcggatgag ggaataacgg
    25201 tagtcgccgt tgagtttttc gttttcataa ttgtaacgaa tcaaagggtt gcctatccac
    25261 atggcgataa aggtcgccaa aatcacaaat atatagacaa accaaacgat gccgtgcgga
    25321 atgtcgaagc cgaacacggt caggatgcca gccaagcccc gcaaaacaac ggcaaattcc
    25381 agagaagtaa cgaccgaatt gaccatgccg cgcacaaatt cgatggtcga agcgataaat
    25441 tcctgcgcgt cctgttggat acgctggtcg atgttgtctg gcgcgtggcg gcgcatttgc
    25501 aggcggtagt agtttttgtc ggcaagccag cgtgctgtca gcacttcgtt gagccgctcc
    25561 gaccatttaa tcgccaagcc ttgatcgagg aagtcgttga cgacgttgtt aaacgcccgt
    25621 atcagcacca cgcccgcgtt cattgcggca aatatccaaa atgccgaagc attcaaatcc
    25681 tgcatcgagt cgtaaagccc tttggacata aaggtactca acacattcag ccgtacttcg
    25741 gttaacagca gcgtaaccat tgccgtaatc agcagcaata ctttgaccgc gtttttcggt
    25801 gtcagacaaa ggcgcaggat ataggcaaac tcccgcccga agcgcgtttt ccgtgtcaaa
    25861 aacagaatca ccgctgaagc ggcagaaact atcaagagag tctgtaaaag ccaaagcggt
    25921 gtggaataaa gctcggtttg ccatttttgc atgggaaatc tcttacggta tcaatgccgt
    25981 ctgaaaagac gggtacagtt gattttttga tgaagtttgg ggaagttttg caggtcaggg
    26041 catattgcgt gttaatttac catacaacca cgccggaatt aagtttaaat ttgaataaaa
    26101 ggttcgggtt ctgtaaaata cagaacccga accttgttcg gatattgaaa ccggccgccc
    26161 gattttgggc ggtgcggctt gcaagtatca agattcgcat atgccgtctg aagctcggag
    26221 aggttcagac ggcatatgct tatttgggct gctcttcgac gaatctcgga cctttcaaga
    26281 tgccgttgtg agaatagggc gacagcaggt tgtatgcggc ggttttggaa acctgataac
    26341 cgcggtcggt caggctgttg gcaatctgat tgaccactgc gctgaccaaa gcccccaaca
    26401 ggccgctgtt gctgttgttg ctgccttcgc ggatgctggc cgaacccgac cacaactctt
    26461 ttccgttgcg ggaatcgacc agccgtgctt tggcggatac ggtcgtcacg ctgtctaaaa
    26521 tttgatatga agtgccgtat tcggtaaccg taatgtacaa aaccgcatca ttgccgaaaa
    26581 tctgatgcag tttttccggc cggacggcgt gaatatcggc ggcattggtc aagccgtttt
    26641 gtttgaaggt ttcctccacg actgcggcgg ggaagacgta atagccggct tcggaaagcg
    26701 gcgcggcggt cgaagccagt acaccccatg ttccgttgac atcgggcgat tcgttcagcg
    26761 gcggaaccac caaaattgaa gccggtttgc tttccttgaa tgacgtgtag tcgaaatcgg
    26821 gcgctttttg aacttggcag gcagacagcg ccaacacggc ggcaagccct aaaatcaaag
    26881 gtttcatcgc ttgcctcctt tacctgtttt catcaggaag tccataaata cgcccgattc
    26941 gggaaacagc cttttctctt cttcaaactg gcggaacgcg ccctctttgt ctcccgaacg
    27001 ggaaagcagc agtcccagat gggcgtgcgc acccggggcg gcattcattt ttttgttgcc
    27061 ggcttccaca aagtattttt ccatcttttc ggtctgcttg cccaacgaag tgttgtcgtt
    27121 tttcaaacct tcatagacgg tatcgggata gccgccgtaa taatacaggg atttttgccc
    27181 gttgccgccg caggcggtca gagccaagac cgccgcacac agcgacaaac ggctcaaggt
    27241 tttcggattc atcatttctc cttaacggtt gggttgccat gcgccgttgt caacagcctg
    27301 aaccagactg ttgacggctt cgcggattgc caagtctaaa actttgccgt tcaaagtcgc
    27361 atcgtagccg gaagtgccgc cgaaaccgat gatttcacgg ttggaaagtg cgtattcgcc
    27421 cgcgccctgt gcggaataga cgatttcgga agtattgacg ttgacgatat tcagagccac
    27481 ttttgcatag gcgatttgcg atttgccgcg acccaaaatg ccgaagagct gatgatcgcc
    27541 gacatctctg cgtccgaatt cggttacatc gccggtaacg acataatctg cgcctttcag
    27601 gttatgcgct ttgccggaaa tgccggattc ctgttttaat gcgatcaaat tggtgcggtt
    27661 cagtacgttg aagcggttgg tctgttgcag gtgcgttacc agaatggttt ttgcctggct
    27721 gcccaaacgg tcttccccgt cggagaaaat gcctttttgg aagctggagc ggttgtcgaa
    27781 tgttccgacg gaaatcgggg tacgaacacc gtgatattgc gtattgtagg aggcgacttt
    27841 tgctacctcg agactgcgtg aggattcggt cgcacagccg gtcagtgaaa cggcagcggc
    27901 ggcaaggaca acggcggtgg aaacggtttt cataaaattt accctaaggt caagttaagg
    27961 aaataacggg ttgtcattat tgtccttatg taaatttaag tcaaggtgtt tgtctgtgcg
    28021 ggacggatgc gcacggaagg tacggatatt tttcaaacgg aatcgcggcg cgggcgggat
    28081 gcctgccttt tccggattta gaggtaaacg atttcgccgc tccgcccttt gctttcggca
    28141 cttgcccacc agacaaatgc gggcagcacg tccccgtagc ttttgcgttc gcttttggct
    28201 tcgcccggat gggatttgat gcgttgcggg gaattgatgg gtccggggac gaggacgttg
    28261 gcgcgcaggt tgccgaagcg ttcccattcg tcggcggcga ctttgcacag gtagttcaac
    28321 gcggctttgg acgcgccgaa gccgccccag taggctttgg gggtttcccc gtggctttcg
    28381 ccgacgaaga taacggatgc gtccggcgac tgtttcagca ggggaaggag ggcgcgggtc
    28441 agccccatag gtgcgacggt gttgatgcgg tattggttga cccattcggc gacggtttgg
    28501 aaatccagcg gcgagagggc gtaaaaatag ccggcgcagt ggacgatgcc gtccagtttg
    28561 ccttgcgtgg cttcggcaat ggtggcggcg aactgattga attctttttc ttccgcactc
    28621 atcaggtcga aacggatggc gaagggttcg gggtgtccgg cttcgacaat cgcgtcatag
    28681 actttttcca gttttttctg atggcgtgcc accagaatca cggttgcgcc tgccgccgca
    28741 taggctttgg cgacctgttc gcccagacct tgcgatgcgc cggtaactaa gatggttttg
    28801 ttggacagtg tcgccatact ttttcctttt tggtggtcgg tcgggggtat tttagcgttt
    28861 tgccgcacct tgtaaagcgt cccgatacca gccggcgttt tcagacggca tcgctcaggc
    28921 tttgcaggca ggtttccgcc gccgcgaaac ctgattgtac ggcggcttcg agcgtggcgg
    28981 ggtagtccgg gtggaggtag tcgccggcgg ggaagatgcg gtgccggtgc agccacgaca
    29041 aatccggcgg cggggcatcg gctgcggttg tggcgcgttt ttcggtgatg acgcgcacgg
    29101 cttcgggttc gcccaaatgc ggaaggatgc gtttgaggtc ggcgtgaact ttgtccgccc
    29161 acgctcggtt tgtaaacgcg ccgacgcggt cggaaacgct gatgacggcg gacacttcgt
    29221 tttcaggcag tccgagcctg ccccggcaaa gcagccattg caccgtgccg tcggcaaggc
    29281 cggtcagcgg ggcgggcagg cggacgggtt cggcgtagcg cagatagacg gtggtgatgg
    29341 cgtggtagcg aaggttttga tatgccgtct gaacgtgttc gggcgtgcct tcgggcagga
    29401 gcgcggcggc gtggtagggc gcggtggcgg ggacggcggc atcgaaagct tcgccgttga
    29461 cgagcacttt cccgtccggg agggtgttca gacggcatac gcgcgtttcg aggcggatgt
    29521 ccgcgccgag ccgttgaaga tccgccaagg cgggttcggc gacgattgcg cccaaatcct
    29581 gcttgggtag gagatagtcg ctgccggatt ttttcgtcag cacgccgtcg gacaaaacgt
    29641 tgcacaacac gcgcaggctt gcggtttcca aaggcgtgtt gagcgcgccc caaaccaagg
    29701 gctgccaaaa ctgcatcacg gcggcacgcg gcacgttccg ctgtttcagc cattgcgcca
    29761 ctgtcgtgtc gggctgtccg aggcgtgcgg acttctgcaa atcggacata tcggcaagca
    29821 gtttggcttt gaatgcagtc ggtgcacgcc gggcaagcag cacgccgccc aaaatatgca
    29881 gcggcgcggg cagggggagg gcgcggaact gcaaaccgcc gtgcatatgc cagtgcagcg
    29941 gtacgcgcaa aaaggcggca cggggatccg aaccgatggt tttcatcagg cgcaacacgc
    30001 cccggtatgc gccgagcaaa atgtgctgcc cgttgtccaa aaaaccgaaa ccgtcggtat
    30061 ttccggccag tgcgcgcgcc ctgccgcccg cctgccggcc ggcttcaaac agggtaacgt
    30121 cggcgtgccg cgccaaggtg acggcggcgg acagtcctgc ccagcctgcg ccgatgacgg
    30181 cgattttcgg gcgcggatgc ggcgtgttca tcatttattc ctccaatggt tgaaaccccg
    30241 tttttcgggg cggcgggggt tgggttttcc tgaagggcgg gcggtatgcg ccttatgccc
    30301 gttccggcgt gccggggcgc ggtttgaatc cgaataacca ggttttcagg gcaatgcgtt
    30361 ttttgcgcgg cgaagggagg gcgattttgt atttgaggac gttttgcgcg ccgtcccggt
    30421 cgatttcgtt caatagctcg taataaaccg ccgccataac cagtccgact ttttgggctt
    30481 ttttatcggc agccggcagc agcgatacgg cttcgcggta ggtttcacgg gcgcgtttga
    30541 tttggaacgc catcaattcg gcaaaattgc ccgtcgggct gcattgcaaa atcacgcttg
    30601 cggggacatc aaaccgctgc atttcctcca tcggcaggta aatccgtccc ctgcgcgcgt
    30661 cttcgccgac atcgcggatg atgttggtca gttgcagcgc aagtcccatc ttgtcggcgt
    30721 attccagcgt tttgccgtct gaaaacccca aaatccgcgc aatcaggcag ccgactacac
    30781 ctgcaacacg gcggcagtac agtttcaatt cttcaaaact gccgtaacgc gcccgaacca
    30841 aatccatctg catcccgtcg attaaggctt ccagttcata tttcggcagc ttgaaggttt
    30901 ccttaacctg ccgcaaggcc tgattgacgg ggtgttccgg catcgcgccg ccgaatacct
    30961 tgtccaaatc gccgcgccac cagttcaatg tcgcctgtgc aacatcgggg ttggaacatt
    31021 cgtcaaccac atcgtccaat tcgcggcaaa aagcatataa aaccgttacc gcatcccgtt
    31081 tttcctgagt caggaaacgg aagcccgaca aaaaactgga gcggctttct tctgcttttt
    31141 ggcggcaata gtcgagtcct ttcacgattt atattcctaa tgatgggagg gaaaggcgga
    31201 ttttatcggc atttggcggt agagggcaat ttcggcggca cgacccaatc cttagcggtt
    31261 tgctcaacta tcggcgcgaa ttctgttaaa atgccgactt ccttccttta cacaccgcac
    31321 cgacaggcag aatttatggc tcttttgcag atttcagaac cgggtatgtc cgccgccccg
    31381 caccgacacc gtttggcggc aggcatcgat ttgggtacga ccaacagctt ggtcgccacc
    31441 gtccgcagcg gcagtgccgc ctgtctgccc gatgccgaag ggcgcgttac cctgccttcc
    31501 gtcgtccgct atctggaaaa cggcggcatt gaagtcggca aaaccgccct gtccgcccaa
    31561 aaaaccgacc cgctgaacac cgtcagctcc gccaaacgcc ttatcgggcg gacgcttgcc
    31621 gatctgcatc aaaatacgca ttacctgcct taccgtttcg gtgacaatca acgcgttatc
    31681 gaactgcata cgctgcaggg ggtgaaaacg cctgtcgaag tgtcggcgga aatcctcaaa
    31741 acccttaaat cgcgcgccga agaaaccttg ggcggcgatt tggtcggcgt ggtgattacc
    31801 gtccctgcct atttcgatga tgcccaacgc caagccacca aagatgccgc gcgtctggcg
    31861 ggtttgaacg tattgcgcct gctcaacgaa cccaccgctg ccgcaatcgc ctacgggctg
    31921 gacaacgcct cggaaggcac gtttgtcgtg tacgacttag ggggcggcac attcgacgta
    31981 tccgtattgc aactgaccaa aggactgttt gaagtcaaag ccaccggcgg caacagcgcg
    32041 ttgggcggcg acgatttcga ccaccgcctg ttctgccgcc tgctcgaaca aaacggactc
    32101 tcccaactca acgaacaaga cagccaactc ctgctctcgc tcgtccgcgc cgccaaagaa
    32161 caattaacca cgcaaaccga agcgcgcatt caggcgacgc tttcagacgg catggcaatc
    32221 gacacaagca tcagtcgcgc cgagttccac aacctgacgc agcatttggt gatgaaaacg
    32281 ctcgaaccgg tcacacaggc gttgaaagat gccggtgtcg gtaaaaacga agtcaaaggc
    32341 gtgattatgg tcggcggttc gacccgtatg ctgcacgtcc aacaggcagt cgccaccttc
    32401 ttcggacaaa ccccgctgaa caacctcaat cccgacgaag tcgtcgcgct cggcgccgcc
    32461 atacaggcaa acgtcctcgc aggcaacaaa accgacggcg aatggctgct gctggacgtt
    32521 acacctttgt cgctcggttt ggaaacctac ggcggcttgg cggaaaaaat catcccgcgc
    32581 aattccacca tccccaccgc gcgcgcgcag gactttacca ccttcaaaga cggtcaaacc
    32641 gcgatgacga tacacgtcgt gcaaggcgag cgcgagctcg tttctgactg ccgcagcctt
    32701 gccaaattca ccctgcgcgg cattccaccg atggcggcgg gtgcggcgcg tatccgcgta
    32761 accttccaaa tcgatgcgga cgggctgctg tccgtttccg cccaagaaca aagcaccggc
    32821 gtacaggcgc aaatcgaagt caaaccttcc tacggcttgg acgacggcgc gattacccga
    32881 atgcttaaag acagtatgga caacgccgcc gaagatatgg cggcacgcgc ccgcgccgaa
    32941 gccgtagtcg aagccgaaag cctgaccgat gccgtcaacg ccgccctcga gttggacagc
    33001 gatttactgg atgccaaaga attgcaacag atccggcaag gcatcgccga tttgcaaggc
    33061 cgtctgaaag acggaaaagc cgaagacatc cgctccgccg tcgccaaact cagccgcagc
    33121 accgacaact tcgccgccaa acgcatgaac cgcaatatcc aacgcgcgtt gactggtcaa
    33181 agcgttgata atatttgacg gcaaatggtt tcagacggca ttggataaag aggtcgtctg
    33241 aaaaaatatc atctcacaga aataaaataa aaaacaacaa tggaaaagtc aggcattgtc
    33301 tatctgatga aaaccgtcat caagggcgtg tataaaatcg gcatttcgga tgtaagcaat
    33361 tttgaaggca gaatgcgcca tttggaaaac aacggttatg cgaacgttgc cgggttggaa
    33421 cgcatcctcg ccgtcaaaac cgacaattac aaagaaaaag aaaacctgct ccatgaaatt
    33481 tttagtaaaa gcaggatagg cgataccgaa ttgttcgccg tggacgagaa tcttgtaaaa
    33541 cgtttgtttt tatcgcttcg cggcgaaatc gtgttcccga aaaacgaaac ggcggaatcg
    33601 gaatttgaaa aaagcgtcca cgaacgcagg caggaaggga atgccggatc cggtcgcaaa
    33661 caactgcttg atttggtacg gcgcggacac cgggaatacc cttacgcgct gccccggctt
    33721 ttggcgggcg cggcatccta caagccgaaa aaatcgaaaa tccgcctttt taaagaagca
    33781 tatttacgca aaagcggtac gaggctgacc gacgaaattg cagacggcat ccatatttac
    33841 acctgttttt cgcgggcgga tttggaaaaa gcctattccg aatatttgga acttttcaaa
    33901 tccgaatcgg atgccgaagg cagaaaacca cggtaaggtg caaacagata ccgtacacgt
    33961 tgaggagcag tatgatgggc gattccgtca tttattatgt agaacaggca gacgaaccgg
    34021 taaaccgtgc cggcgaacgc gcccgtaaaa cattcaaata cttttggcgc gagctttttt
    34081 gggaacgccg ccgcatcatt cccgccttag attttgccat ggtcaaagcc ccttttttcc
    34141 aagacggcga agacggcgaa atttgcgaac atatgtggat cgacgatatt tattttgacg
    34201 gtctttacat ttacggtgtg ctgaacaata aacccggcga actgactaat gtcgaacaag
    34261 gcgaaagcat ttgcgttccg gttgacgaca tcagcgactg gatgttcgtg tgcaacggca
    34321 ttccctacgg cggctttacc atacaggcaa tgcgtggaca gatgacggaa gaggagcgta
    34381 ccgaacacga tgccgcatgg ggaatcgatt tcggcgatcc cgggcaggta ttgctggtgt
    34441 atgaagaaaa agaacatccc gaaaatctgg aagagcatcc gatgtgccgg aactgtattg
    34501 acgattttcg gcaacagttg tcccaaaatc cggattttct gcatgagcag gacgaagacg
    34561 gctatacgcc gcttcatcat gaagccatcg caggaaatgc acttatggtt caagccatgc
    34621 ttgaatacgg cgcaaatcct gcctcaaaga catcggaagg ctataccgcc ctcgattttg
    34681 cccgcctgac gggctggcaa aatgttgccg acctgctcga accgcgacat taggcagaca
    34741 gttttccgaa aacgaacaca aacacttttt acagaaagac aataaaaatg cccaaaatca
    34801 ccgtacttcc acacacgaca ttatgccccg agggtgcagt catcgataac gcacccgaag
    34861 gtaaaaccgt ccttgacgtg ctgctcgacc atgatatcga agtcgatcac gcctgcgaaa
    34921 aatcctgcgc ctgcacaacc tgccacgtga ttatccgcaa aggtttcgac agtctggaag
    34981 agccgaccga attggaagaa gacctgctcg atcaggcttg gggtttggaa gccgattcgc
    35041 gcctgagttg tcaggcggtt gtcgccggcg aagatttgat tgtggaaatc cccaaataca
    35101 ccatcaacca cgcgcgcgaa gaacactgaa aacaggccgt ctgaagccgg cacgcttcag
    35161 acggcattgt tgcgcggata aggcgcaatc gcccgaaaac aggcgttcgt acaggcggaa
    35221 ctttcgattc tatagcgaat taacaaaaat caggacaagg cggcgagccg cagacagtac
    35281 aaatagtacg gcaaggcgag gcaacgctgt accggtttaa atttaattca ctatatattg
    35341 atttttatcg gttttctgac ggaacggatt ggtgcggcat ccgaggcgga ttactcgggc
    35401 gcgatgcacg ggtatttatc ggttttgcag ccggaaaaac cgccggcggg ttactttcag
    35461 ggcggcggtc gggcggtatg ccggatagcg ttccactttc gcttctatat tgattttgat
    35521 tggttttctg acggaatgac ccgatgcggc atccgggacg gcttgtgttt tttcctgccc
    35581 gcctgccgga ttttcccatc cttgcgtgaa accgaaagag acggcggcgc ggcggacaag
    35641 ctcgagatag cgtccttcaa gctccgggca ggcggcggac acgctttctg ccgtaaccgt
    35701 gaagccgccg cccgacccgg aaaggcgttc cgcaatctgc gtgtagatac gccaacgttg
    35761 ggcgcgcaac attgccgcat cgcaaccggc agccgcttta tccaaggcaa tcaggccgcg
    35821 ccggttttgg tggtgaaggc aaaccgttac tctggagagg atatgttgcc cgtccaatat
    35881 ttgatacagt ttgcgtatca gcagaatcag gcggtcaaac tcctccatct ccgacaggga
    35941 atcaggatgg tcggaagcgg tataaaccag tttggacaat tttgcggcaa acatattgtt
    36001 catcaatctt ccttgtcggt tgaaaccccg ctcttcgggg cggtagaatc agatttgttt
    36061 gggagggaca actcctccca gatcaggacg acacataggc tggtgcttga tgtgttgtcc
    36121 ggcgagttga aacattcagc catcctcaag gtgcggcagt tttgccgaaa catattctac
    36181 acggcttcaa tgccggacga taaaaggaaa ttcatatgaa atggaccgac acccggcgta
    36241 tcgccgaaga actctatgac ctgcacggcg aaaccatcga tcccagaacc gtgcgcttta
    36301 cccaactgcg cgacctgatt atggcattgc ccgaatttga cgacgacccc gcccgttgcg
    36361 gcgaacgcat cctcgaagcc gtgcagcagg catggataga cgaggcggaa taagtttcgg
    36421 gaatgccgtc tgaaatgcgg cggtacgcgg ttcgtgcttc tgtttgcagc gggaatggtt
    36481 ttaccagtct ccttttttca gcctgtccag ttggcggcgg tcgcgcttgg tcggtctgcc
    36541 gtcgggatag gcggaagtga tgcggctgaa ttggtcgagc tgtttgcgct cttccctcaa
    36601 tgttgccgtt ttcgcgtcct cttcatacag aagccgcgcc tcggatgccg ggcggcgttg
    36661 gtggttcaaa cctttaacct tgattttata gggaagggaa ttgagcgtca ggtcgataat
    36721 atcgccgatg tctatggttt tactgttttt gaccttcgag ccgtttactt gaaccctacc
    36781 cagttcgatg tgcttttgcg caagggaacg ggtcttgaaa aaacgtgccg cccaaagcca
    36841 tttgtccagc cgcatggcgg aagaatcgtg cttgtctttc atacgatttt gtttgaaata
    36901 attgaatttg tttcaagttt agcataagat acgccgccta taactagtat atatgcacta
    36961 atccactgtt ttccatgctg tccgaacaca aaaagagggt gtggaaaagc cgttttggac
    37021 aataaattaa ctgcggaata tgcacaaata gcgtatgata gcggcagaat ctgttgatga
    37081 gagcttcatt ctatgaaacc tgtttttttg gattttgaac aacccatagc cgaactgacc
    37141 aacaaaatcg atgagctgcg tttcgtccaa gacgagtctg ccgtcgatat ttcggacgaa
    37201 atacaccgtt tgcagaaaaa aagcaacgac ctgaccaaat cgattttcag caaacttacg
    37261 cccgcccagg tttcacaggt ttcccggcat ccgcagcgtc cctatacttt ggattacatt
    37321 gaggcactgt ttactgattt tgaagagctg cacggcgacc gccactttgc cgacgattat
    37381 gcgattgtcg gcggattggc gcgtttcaac ggacaaagcg tgatggtcgt cgggcatcag
    37441 aaagggcgcg acaccaaaga aaaaatccgc cgcaacttcg gtatgccccg tcctgaaggc
    37501 taccgcaaag ccctgcgcct gatgaagacg gcagaaaaat tcggcttgcc cgtcatgacc
    37561 tttatcgata cgccgggcgc gtatcccggc atcggcgcgg aagaacgcgg acagtcggaa
    37621 gccatcggca aaaacctgta cgaactgacg cgcctgcacg tccccgtttt gtgtaccgtc
    37681 atcggcgaag ggggctcggg cggtgcgttg gcggtcgccg taggcgatta cgtcaatatg
    37741 ctgcaatatt cgacctattc tgttatctcc cccgaaggct gcgcttcgat tttgtggaaa
    37801 accgccgaaa aggcggcgga tgcggctcag gctttgggca ttactgctga ccgcctgcaa
    37861 aagctggact tggtcgatac cgtcatcaaa gaaccattgg gcggcgcgca tcgggatttc
    37921 gggcaaacca tgaaaaacgt aaaagccgtt ttggaaaaac aactgcacga agcgcaaagc
    37981 atcccgcttg ccgatttgct ttcgcgccgt ttcgaccgca ttatggctta cggcaaattt
    38041 tcggaacaat aattcaggta gaacaagcag caagcagttt gtctgaaact gcttgctttt
    38101 tctttatcgg gacggaaccg tgctgacttt agatgcgttt gagcaatgct tgaaggattg
    38161 ttttcctcaa ggtctgaatg gaaaaaaaac agcggtggca ttaagcggcg gcttggattc
    38221 cgtcgttttg ctgcatctgc ttgtccgcgc cgggaaaaaa ggcggtttta ttccggatgc
    38281 attgcatatc catcacggct tgagtccccg tgccgacgat tgggcggatt tctgccaaaa
    38341 ctattgcgat atgctcgggg tggggctgga aacggttaag atctgcgtgg aaaaaaacgg
    38401 tttgggcatc gaggcggcgg cgcggcaaaa gcgttatgcc gcgtttgccg aaaaaggctt
    38461 tgacgttttg gcgttggcgc accacaggga cgatcaaatc gaaaccttta tgctggcggt
    38521 cgcgcgcggc ggcggtttgc gcgctttggc ggctatgccc gccgtccgcc cttttgggga
    38581 aaaaggcatc atttggcggc ccttgctgcc tttttcgcgc caagatatat gggattatgc
    38641 ccaaaaacac ggtttgccga atatcgagga cgaaagcaat agcgatacgg cttatttgcg
    38701 aaaccgcttc cggcaccgta ttttgcccga actttcggcg cagattcccc atttcgggcg
    38761 gcatctgttg aacaatgtcc gtgccttgca ggaggatttg gctttgttgg acgaggtcgt
    38821 cgttcaagac tgccgttggg tttgcggagc cggttatttc gatacggcgc ggtggctgac
    38881 gttttccccg cgccggaaaa cccatatttt gcggcatttt ttgaaggaaa acggcattcc
    38941 cgtgccgaat cagaatgccc ttgccgacat tgcccgggtt ttgacggagg caaaaaccgg
    39001 acgttggaac gtgcaaggct ttgaattgca tcattatgca ggcaggctgt ttgtgttccg
    39061 accggaaaaa acggataaac tgcggttttt gaaagacagg cagataagcg gaaatttaag
    39121 ggaaatattg gcggggcagg gatttgtttt aaaacgtcat ccgttcggac ttcccgggca
    39181 tcttttggag caggacggaa tcttgcggac ggttgcggca tcggacaggc tggctgtcgg
    39241 cggtatccat aaggatgtga agaaaatcct tcaggaaaag cgggttttgc ccgcgctgcg
    39301 cccgttttgg ccggtcgtcg ccgacaagga caaccgtccg ctggcgttgg caaattacgg
    39361 tacggatttt caaatctcgg tttcagacgg cattttgccc gtccatccgg attttcctgt
    39421 tttattttga tggaatcgga atcgccgtta cctattatat attcttgaaa aggcggtaaa
    39481 gtgaagcata tcagttcggc aaacaatgaa cacatcagac acctgcaccg cctgttgtct
    39541 caagggaagt tcagacggca atacgcccaa accgttttgg agggcgtgca cctgcttcag
    39601 gttttcctgc aatccggcgg aaggccagtc ggggtatata ttcccgaagc gaaaatgccg
    39661 tctgaagaag tccgtaaatt gacggcggtt ttgccggaag acggggtttt ttccgtttca
    39721 gacggcatat tgaagaaaat cagcagcctg acttgcgcgg atgatgtgct tgcgctgatt
    39781 gatattccgg atgcgggtgc tttgccggcc ggcggcgact gtgtggtttt ggacggcgtg
    39841 caagatccgg gcaatgtcgg cacggtgttg cggagcgcgg cggcggcagg aatcggcgcg
    39901 gtcgttttgg gcaaaggttg tgcggacgcg tggtcgccca aagtgctgcg agccggaatg
    39961 ggcgcgcatt tcttgtcgga gatttatccg caggcggatt tggaaatatg gttggcgcgt
    40021 tatgaagacc gtgtgtttgc caccgcgttg cgtgaggaaa aacaggcggt tctgtataac
    40081 gaagatttgc gcgaaccgac agcctgggtg tttggcaacg aaggcgcggg ggtcggtaaa
    40141 gcagttttag acagggcgga caagtgtgtc aggataccga tgcacgatgc aaccgagtct
    40201 ttaaatgtcg cgatggcggc gacaatctgc ctgtttgaac aaatgcgcca acgggcggcg
    40261 tattgaggaa aagaaatgcc gtctgaaaaa atctattacg gcgtattgat tttcttatgt
    40321 atcgcttcta tgctgctgtc gccgtttttt tatgcgggtg ctttgaagcc caagaaggcg
    40381 gcattgcgga aggacgggca gtggaaactc atcttattgt ccaataccgt ggcggcggcg
    40441 gttttggctt gggtgtggtg gaaatggttt tgacagatat tgctcaaaat cgtgctaatg
    40501 gaatccgaac aaataaagaa tttggtaaaa aatttgttaa atcaacgaat taaagttttg
    40561 tggaaaacaa aacagctcta agcaaatagg gcgtttgtcg gtaaatacgg aagagttgcg
    40621 gcattatcgg gcatctttaa caagtagtgc cgtcttgaca ggcaatcggt ttttatgggc
    40681 agcttgcaaa atcgcggata taaaattgca aatcggttaa agtgtgggga cgctatgaaa
    40741 aattgcgaat ttttttatga cccgacaagg gcaatctatg atagcggtgc agattattta
    40801 accagagaaa aacatagatt cgtcgtaatt gcaaatagtg cttgggggct attgcttaat
    40861 ttatcttgtt attatgacga ggttttggaa aagcggaaaa taccgttcgg caaacaggaa
    40921 attgatgacg atatggacaa agtgtccgcc cataagcgga agtttaaaga tatttctgaa
    40981 atcaaagtag gggatggttg ggaatacccg ttcaattatg agcagggaat gaaagaatta
    41041 gatgaagtgc tattgaaata cattcccttt tttgaagaag aacgataaag gaggttgata
    41101 tgcgcgtatc taaaataatt ggaagtatgt tgcttgttac agcggttcag accgtatttt
    41161 cggcaaatgt ttacgcgtgc cgccataatg gtaaaaccag ttacagccaa actccgggaa
    41221 aacattgtac caacgcgggt ttggggggcg ggaccgggtg tacagttcgg ttagaccggc
    41281 agtaaaagac agggcggaag acgcaggagt tggcgattat tcggacacgg tgagggacga
    41341 acacgtccaa attccgaaag ggaatgcaca gaaagacggt tcggatgccg gcatcaggcc
    41401 gcattgattg aaaccgaatc aaccatgacg gtgttgaaca atagaatttg aacgattagg
    41461 gaactctgat gaaacacatc cacattatcg gtatcggcgg cacgtttatg ggcggagtgg
    41521 ccgccattgc caaagaagcg gggtttaaag tcagcggttg cgacgcgaag atgtatccgc
    41581 cgatgagcac ccagctcgaa gccttgggca taggcgtaca cgaaggcttc gacgcggcgc
    41641 agttggagga atttaaagcc gacgtttacg tcatcggcaa tgtcgccaag cgcgggatgg
    41701 atgtggttga ggcgattttg aaccgcgggc tgccttatat ttccggtccg caatggctgt
    41761 cggaaaacgt gctgcgccat cattgggtac tcggtgtggc ggggacgcac ggcaaaacga
    41821 ccaccgcgtc catgctcgca tgggttttgg aatatgccgg actcgcgccg ggcttcctta
    41881 tcggcggcgt accggaaaac tttagcgttt ccgcccgcct gccgcaaacg ccgcgtcaag
    41941 acccgaacag caaatcgccg tttttcgtca tcgaagccga cgaatacgac accgcctttt
    42001 tcgacaaacg ctccaaattc gtgcattacc gtccacgtac cgccgtgttg aacaatctgg
    42061 aattcgacca tgccgacatc tttgccgatt tgggcgcgat acagacccag ttccatcacc
    42121 tcgtgcgcac cgtgccgtct gaaggcttaa tcatctgtaa cggacagcag caaagcttgc
    42181 aggatacttt agacaaaggc tgctggacgc cggtggagaa attcggcacg gaacacggct
    42241 ggcaggccgg cgaagccaat gccgacggct cgttcgacgt gttactagac ggcaaaaccg
    42301 ccggacgcgt caaatgggat ttgatgggca ggcacaaccg catgaacgcg ctcgccgtca
    42361 ttgccgccgc gcgtcatgtc ggtgtcgata ttcagaccgc ctgcgaagcc ttgggcgcgt
    42421 ttaaaaacgt caaacgccgc atggaaatca aaggcacggc aaacggcatt accgtttacg
    42481 acgactttgc ccaccatccg accgccatcg aaaccacgat tcaaggtttg cgccaacgta
    42541 tcggcggcgc gcgtatcctc gccgtcctcg agccgcgttc caacacgatg aagctgggca
    42601 cgatgaagtc cgccctgccc gaaagcctca aagaagccga ccaagtgttc tgctacgccg
    42661 gtggcgtgga ctggaacgtc gccgaagccc tcgcgccttt gggcggcagg ctgcacgtcg
    42721 gcaaagactt cgatgccttc gttgccgaaa tcgtgaaaaa cgccgaagca ggcgaccata
    42781 ttttggtgat gagcaacggc ggtttcggcg gaatacacac caaactgctg gacgctttga
    42841 gatagcccgg gcgatgccgt ctgaaagccc ttcagacggc atcgcccggc tgcgcggcac
    42901 aaaggcggaa aaaccgtttg ccccgtattt tcaaacgcgt tacacttgcc gccgctgttt
    42961 tcagccattt gattacccgc aaccgccgtc attgcgccgg cggtttgcct gtcagcgtca
    43021 ttgcgccgct gtaaatacga aagaacacat tatgaccgta tcccccgtcg ccttgcgccg
    43081 taagaccgag tgcaagcctc atcccaccgc gcgctattgg aaaaaatgcg atgtcgaagc
    43141 cctgttcgga cttcccttcc tcgacctcat ttaccaagcc gccgaaatcc accgccaaaa
    43201 tttcaacccg cgcgaaatcc agctttccac gctgttgtcc atcaaaaccg gcggttgtcc
    43261 cgaagactgc gcctattgtc cgcaatcggc gcaccacaat accaatctgg gcaaagagca
    43321 gatgatggat gtggatgaaa tcgtcgaaaa agccaaaatc gccaaatcgc gcggcgcaag
    43381 ccggttttgt atgggcgcgg cgtggcgcgg ccctaaaccc aaagacgtgg agacggtttc
    43441 cgcaatcatc aaagccgtca agggcttggg tatggaaacc tgcggcacgt tcggtatgct
    43501 cgaagaaggt atggcggaag acttgaaaga ggcgggcttg gattattaca accacaacct
    43561 cgacaccgac cccgaccgct acaacgacat catccacacc cgccaacacg aagaccgaat
    43621 ggacaccttg ggcaaagtcc gcaacgccgg tttgaaagtc tgctgcggcg gcatcgtcgg
    43681 gatgaacgaa acccgcgccg aacgtgccgg gctgattgcc agtctcgcca atctcgaccc
    43741 gcagcccgaa agcgtgccga ttaaccggtt ggtcaaagtg gaaggcacgc cgcttgccga
    43801 tgccgaagat ttggactgga cggaatttgt ccgcaccatc gccgtggcgc ggattacgat
    43861 gccgcaaagt tatgtccggc tgtcggcagg gcgcagcaat atgcctgaag caatgcaggc
    43921 gatgtgcttt atggcgggcg cgaactcgat tttttacggc gacaagctgc tgaccacggg
    43981 caatcctgat gaggacggcg acagaatcct gatggaaaag ctcaacctgt atcccttgca
    44041 gtttgaaccg gaaggcgagg tcgccgaagt ggaaaaagcc tctgggatta aagtggatta
    44101 ttgacgattg aaaaatgccg tctgaaaccc ggaaaaaggc tttcagacgg catttgtccg
    44161 gacggcattt ccaatatctt tttaccggcg cgtgatgctg ccgtcgggcg agacatccag
    44221 cccgttcccc ttggggaaat cgctgcggtt cagataaata atccgcccga taacggtttt
    44281 ctcggggtcg attttgggga ttttatcgtc ggcttgggta atagggatga tagttccgga
    44341 aataaattta ccctgtttgt cggtgataat tttgaaaatc ggcgcaatgc cgctgatgcc
    44401 ggaggtgttg attgcgccgt aggtggcaaa gttgccgccg ctgtaggaga tgaagcggtc
    44461 gcggtaaagt tcgacggcgc gtgtaacgtg cggcccctgc ccgaatacga catccgcgcc
    44521 ggaatcgacg gcaagccgtg caaactcgac gacgttgccc ctgttttccc catagaagat
    44581 ttcggtatcg aacggcaggt gttccgcctg tttcccttcc gcgccgccgt ggaacatcac
    44641 aataacgatc tcggcttttt gtttggtttt ggcaatcagt tttttgactt tggcatagtc
    44701 gttcagtttg acggcggcaa ggttgggggc gaaggagacg aagccggatc ttacgccgtt
    44761 tttcttcagg atggcggttt caaacctgtt ttcgatgccc gaatatttga tgttcaattc
    44821 gtcaaggttc gccctttatg ccgtttccgc accgcaaacc gccggggtca agccctcggc
    44881 gcatcctgcc ggaacggaat ccccgtgctg ccgattgacg gttcaaaccc cgcgcccgtt
    44941 tcaaataccg gcgatgtgga cggacaggat gcgcctgacg aaaagacagc cgataccgtt
    45001 tccattatcg gcgtgggcga cattatgctc ggcagcaatt atccggtcga ttacctgccc
    45061 gataccaata ttctgaaaaa cgtcgaatct gccttgcaag acgcggacat taccgtcggc
    45121 aacctcgaag gcacgctgtt tgacgaaggc ggtacgccga aaaaatgtgc aaccccccaa
    45181 aatatgctat gcattccgaa cgccctccgc atacgggcaa taccttgccg acgcgggatt
    45241 cgactacctc agcttcgcca acaaccacag caacgacttc ggcgcgcaag gcatcacggc
    45301 aacggcggcg agcggcagct cttttacata ctcgatcgcg ctaaagccgc tgccgataac
    45361 gaggccaaaa ttgcggaaaa taccgccatc gcccagataa atttgtccat catcagacct
    45421 ttactgttca gacgagacag catttgccgc acgttttggg gcttatcttt cgatttgcgc
    45481 tacgtcgcgc accgcgcctt tgtcggcgga agtcgccatc gcgccgtaag ctcttaatgc
    45541 ggcggagacg tagcggtcgc ggttttccgg tttccacgct ttgctgccgc gtgcttccat
    45601 ttcggcacgg cgggcggcga gttcttcgtc ggaaatgaca aggcggatgc tgcggttggg
    45661 gatgtcgatt tcgacggtat cgccttcgtg taccaaaccg atcgcgccac cttccgccgc
    45721 ttcgggcgag gcgtgtccga tggacaaacc tgatgtgccg ccggagaagc gtccgtcggt
    45781 taagagagcg caggctttgc cgaggccttt agatttcagg taggaagtcg gatacagcat
    45841 ttcctgcatg cccgggccgc ctttcgggcc ttcgtagcgg atgatgacga tgtcgccggc
    45901 gacgatttgg ttgcccaaaa tgccttctac tgcgtcttct tggctttcaa acacgcgggc
    45961 gcggccggtg aatttgagga tgctctcgtc cacgcctgcg gtttttacca cgcagccgcg
    46021 ctcggcgatg ttgccgaaca agaccgccaa accgccgtct tgcgagtagg cgtgtgccac
    46081 gtcgcggata cagccttttt cgcggtcgag gtcgagggtt ttccacatac ggttttgcga
    46141 gaacgcttgg gtggtgcgta cgccgccggg cgcggctttg aagcgttcga tggcgtgggt
    46201 gttttcaggg ttggttacgt cccatttttc aatcgcgtct ttcagcgtcg gcgcgtggat
    46261 agtgtgcacg tcggtgtgca gcttgcccgc tttgtccaat tctttcagga tggcgaagat
    46321 gccgccggct cggtgcacgt cttccatata gtagtcgtgg ttgttgggtg cggttttgca
    46381 gatgcagggc acaacgcggc ttaagcggtc gatgtctgcc attttgaaat ccacgcccgc
    46441 ttcgttggca acggcgagta aatgcaaaat agtgttggtg ctgccgccca tggcaatgtc
    46501 catggtcata gcgttttcaa acgctttttt ggtggcaatg ctgcgcggta acacggtttc
    46561 atcgttttgc tcgtaatagc gtttggtgat ttcgacaatc atacggccgg cttcgaggaa
    46621 cagttctttg cggccgacgt gggtggcgag gtaagaaccg ttgcccggca gggacagccc
    46681 gagtgcttcg gtcaggcagt tcatcgagtt tgccgtaaac atacccgaac acgagccgca
    46741 ggtcgggcag gcgttttgtt cgacttcttc aacttgccgg ttgctgatat tgtcgtccgc
    46801 cgattcaatc atcgcgtcaa tcaagtccaa acggcgttcg ggctggatgt ttgccacgcc
    46861 gataacttta cctgcttcca tcggaccgcc ggaaacgaag atggtcggga tgttcaggcg
    46921 catcgcggca atcagcatgc ccggggtgat tttgtcgcag ttggaaatgc acaccagcgc
    46981 gtcggcgcag tgggcattga ccatatattc gatggagtcg gcaatcaaat cgcggctggg
    47041 cagggaatac agcataccgc tgtgtcccat agcgatgccg tcgtcgatgg cgatggtgtt
    47101 gaattctttg gcgatggcgc cggctttttc gatttcgcgg gcaaccagct ggcccatatt
    47161 gtgcaggtgg acatgacctg gcacgaattg ggtgaacgag ttggcaacgg cgataatcgg
    47221 tttgccgaag tcggtttcca tcacgccggt ggcgcgccac aacgcgcgcg cgcccgccat
    47281 attgcgaccg tgggtggagg ttttggagcg gtattctggc atagtgtgtt tccttgtgcc
    47341 tataccgtct gaaagacagg gctgtttcag acggtatcgg gtacggtttt ttagagtggg
    47401 aaaagagggt attttatacc aagtatcgga attttgcggg attgaaacgg cgtgcggcaa
    47461 aaaagaaaat ccccgcagga atgcggggac gggttcaggc gcgggcaatc gcgacggctt
    47521 tggatgcgtc ccaaaaatca acgggtgcat ttaatacggg tttgacgatg cccgtccgta
    47581 tggcgaaatc gccgaaacct tcgccgatat tgcgttctgc cgcccatttg ccgatcaggt
    47641 cgtccaattc ggcaaggatt tcgggcaggg tgatgttttc tttgtaaaga cgggggatgc
    47701 gtacgccttc acggtcgccg ccgatatgga ggttgtagcg tccgacggct ttgccgacca
    47761 gtccgatttc cgccaacatc gcccgtccgc agccgttcgg gcagccggta atgcgggtaa
    47821 cgatgtagtc gtccgacgtg ccgtgtttcg ccataatctt atccagctcg ccgatgaagt
    47881 ccggcagcac gcgttcggct tccgccattg ccagcgggca ggtcgggaag gaaacgcacg
    47941 acatcgcatt ttcgcgcagt ttgctgacac cgtcgtgtat cagcccgtat gagcgggcga
    48001 gctgttcgat ccgggctttg cctccttcgg gtacatttgc cacgatgagg ttttggttgg
    48061 cggtgatgcg gaagtcgcct ttgtggattt tggcgatttc caacacgccg gtcagaagct
    48121 gtttcccgcc ttcgtcaacc aaacgcccgc tttcgatgaa aagggtcaaa tgccagttgc
    48181 cgtctatgcc tttcacccag ccgatacggt cgccgcgccc ggtaaatttg aacgggcgta
    48241 cgggttcgaa cggcataccc atacggcgtt cgacttcggc gcggaagttg tccaagccca
    48301 tattttgaat ggtgtagcgg gtgcgggcgt ttttgcggtc gctgcggttg ccgaagtcgc
    48361 gctgcgtggt taccaccgct tcggcggcct tcagcgcgtg ttccggaggc acgaaaccca
    48421 gttccagtga aatgttcgga taggttttgg tgttgccgtg ttccatcgaa agcccgccgc
    48481 ctgccaaaac attgaagccg gcaagctgtc cgttaccgtc tgaaacggcg acgaaatcca
    48541 aatcgttgcc gtagcagtcc acatcgttca agggcgggat gacgactgcg gttttgaatt
    48601 ttcgcggcag ataggttttg cccaaaatcg gctcgtcttc ttgaaggaag tcgtcggaac
    48661 tttgaacttt tttgccgtcc acccacacat ccagataacc gcgcgtgcgt ggcaataggt
    48721 gttcggaaat tttttttgcg tattcgtaag cctgttggtg gagtgccgac tcgatgggat
    48781 tggaggtgca aagcacgttg cggttcatat ccgccgccgt ggcgatggaa tccaaaccca
    48841 gtttgtgcaa gaggcggtgc atcgtctgca acttggtttt cggcacgccg tgaaattgga
    48901 aggtttgccg gttggtcagc cggatggagc ggtaatgact gttttcccgg gcaaatttgt
    48961 ccagttctat ccattgggac ggtttgatga tcccgcccgg cagtcggcag cgcaaaagca
    49021 tgaatttcaa gggctcgagt tttgcctcgg cgcgttcggc gcggatgtcg cggtcgtcct
    49081 gctcatacat accgtggaag cggatgagtt ggaagttgtc gcctttgaag ccgcccgtga
    49141 gcgggtcttt caaatcgtcc aaaatcgtgc cgcgtaaaaa attgctttcg gttttcagac
    49201 gttcgttgtc ggatagcggt ttttcttgcc acgccaaacc ttttgtcttg gtctgtacgg
    49261 tcattttgtg ttcctcccga ttatatttaa tcaataaaca tcacgctgat agcgtttttc
    49321 ttcgcgcagc atatccaaat attcttctgc gccctcttcg tccaaatgtc ctgccccgat
    49381 aatcacatcc agcaaggcgg cttccacgtc ttttgccatt tttgccgcat cgccgcacac
    49441 atagatatgc gcgccttcct gcagccattg ccaaagtcct tccgcctgtt cgcggatttt
    49501 gtcctgcaca tagatttttt cttcctgatc gcgggaccag gcgaaatcgt acctgtgcag
    49561 gaagccgtct ttggcaaact gctgccattc ggtctgatag aggaagtcgg cggcaaaatg
    49621 cggattgccg aaaatcagcc agtttttgcc ttccgcattt tctgcggcac gttgttggac
    49681 gaaggcgcgg aacggtgcga cgccggtgcc cgagccgatc atcacaatcg gcttgcggct
    49741 gtcttcgggc agcctgaagc cgtcgttgcg ttccacaaac acgcgcaccg tgccgtcctc
    49801 ttccagccgg tcggcaagga aacccgatgc gccgcccgcc ctggcgcgcc cttcgtgttc
    49861 gaaacgcacc gcgccgacgg tcaggtgcac ttcgtccccc acttccgcct gcgacgagga
    49921 aatcgaatac aggcgcggcg caagcgggcg cagtaggccg gcgaattgtt ccgccgtcag
    49981 ttttgccggg aagcggtgca gcacatcggc aatcggcgtg ctttgcacaa agccttgcaa
    50041 aacggcgttg tcggcagcaa tacggtcgag ttcgtcatca tcggcgaacg gggcatagcc
    50101 tttgacaaag gcgggggtgt tttgcgtgag ttcaaaatgg gataacagtg cggaggcaac
    50161 cggcagggtt tttccgcccg cctgtatttc cgttgccgga tcgatgccga gcaggtctag
    50221 gatttccctg accagtgccg gatcgttgtc aaaccaaacg ccgagcgcgt cgcccggtag
    50281 gtagtgcaaa tccgaaccgc tcaaatcgat ttcgatgtgg cgcacgtctt tatcggattg
    50341 gcgggcggta attttctgat tggccagcag ggcggcggga aagggggctg ccttgcagta
    50401 cctgccctcc gatgccgtct gaaggccggc ggggggcgtt gcctgcggcg cgggcgttgc
    50461 ccggtttttt gcggcttctt cttttaagag tgcggcgata ttgtctgtcc aggcgtttgc
    50521 ggcggcggca aagtccaaat ccgcatcaac gcgttcgagc aggcgtttcg cccccaattc
    50581 ttcaaaacgt ttgtcgaaat ctttgcccgc ccggcagaaa ttcggatagg aactgtcgcc
    50641 caaacccagt acggcaaatt ggagtttgtc caatttcggg gcttttttgc cgttcagcag
    50701 tttgtgcagc acgacggctt cttccggcgg ttcgccttcg ccttgggtgg acgtaaccag
    50761 cagcaggcgg cgttcgccgg cgatgttttt cgccttatag tctttcagtt cggcgcgact
    50821 gacttggatg ccggcggctt ccaggctgtc cgccgctttg tcggcaacgg atttcgcatt
    50881 gccggtttgc gaggcggaaa ggacggttac ggaaaaaggt tctgccgtcg gcaatgccgt
    50941 ctgaagcgcg ggcagtcctg cagatgcccc gtttcctgct tttgcccaag cgtagccgga
    51001 cagccacgcc cattgtgccg cgttcagccc cgacaggagc tgcgtgattt cgggcggcat
    51061 aggcggtaat ggcggatttg tgttctgcat atcgtgttca ctcataaaat catacctgcc
    51121 gcaacagtgc cgtatgtcgc ttcgtctatc aggataaacg aaccggcggc ggtgttttcc
    51181 gcataaggcg ttgccgtaac gggtttttga aggttgatgc ggactttggc gatgtcgttc
    51241 atcttcaagg attccgcgcc ggcctcttgt tccagcgtgc ggacatccaa aacgctttca
    51301 atttccccga cttttgccgg cacggtttgc gtgccgtgct tgagcaggta tttgcgcgcg
    51361 gtgttgagcg gacgttcgtc aaaccagcaa agcgtggctt ccagatgttt ttgcggggcg
    51421 agcggggaat ttttatcgac aaaaaggtcg ccgcgcgaaa catcgatgtc gcgatccagc
    51481 cggagggttg ccgcctcgcc ggcaaaagcc tgcgccactt cccctttcgg cgtgatgatt
    51541 tcggacactt cggcggtcag cccgttcggt tcgatgcgga cggtttgccc gacggcgacc
    51601 gaaccgcgtt cgatgcgccc ctgatagcct cggaaatcat cggccttgtc ggcatcttgg
    51661 cggacgacca gttgcacggg gaaataaaaa tcggcagcgg aacgaccggt ttcgtccgcc
    51721 cccggcaggt tttccaagat ggccagcagc ggttcgcccc gataccacgg catattgccg
    51781 ccggggtaaa cgaggttgtc gcccaagagt gcggacaccg gtacgaaatg cacgtctttc
    51841 aaaccgagcc gtccggcgag ccggcggtat gcctccacaa tggcgttgaa tttgtcttcg
    51901 ctgtaatcca gcaggtccat tttgttgacc gccaccacaa tatgcgggca gtcgagttgg
    51961 cggaggatgg cggaatggcg tttggtctgc ggcagaagct gcaagggctg cgcgccgaaa
    52021 tccagttggg atgcgtcaac cagcacgact gccgccgaag cggtgcttgc gcccgtaacc
    52081 atattgcgcg tgtattgttc gtgccccggc gtgtcggcga tgatgaattt ccgtttcgcc
    52141 gtggaaaaat agcggtatgc cacatcgatc gtaatgccct gttcgcgttc ggcttccagt
    52201 ccgtcggtca ggatggagaa gtctatggtt tctttcaaac ctttgctttt gccggattcc
    52261 aaggttttga tttggtcgga cagcagggct ttgctgtcgt agagcagtcg tccgattagg
    52321 gtgcttttgc cgtcatcgac gctgccggcg gtaatgaagc ggagcggggt ttggtgttgt
    52381 gccgtcatat tttcttcctc atatctgctt aaagggtttt tgaaatttag aaatagcctt
    52441 cttttttgcg tttttccatt gccgcctcgc ttgcctgatc gtccagccgg gtcgcgctgc
    52501 gttcggaaat gtcggcaacc gctgtttctc tgataatctc cgtcggcgtg gacgcggtgc
    52561 tttctaccgg gcaggtgcag ctgatgtcgc cgacggtgcg gaagcggaca tcaaggattt
    52621 cggaggtttc agacggcatt ttcggggtga gcggcgttac ggggaccagc agccccctgc
    52681 gtctgaccac ttcgcgcctg tggctgtaat aaatcggcgg cagctcgagg ttttcgcggg
    52741 cgatgtattg ccagatgtcg agttccgtcc agttggaaat cgggaagacg cgcatatttt
    52801 cgcctttgtg cagcctggtg ttgtacagcg accacagctc ggggcgttgc gccttcggat
    52861 cccattgtcc gaactcgtcg cggaacgaga aaatccgttc tttggcgcgg gctttttctt
    52921 cgtcgcgccg cgcgccgccc ataagcgcgt cgaagccgtt tgcctcgatg gtttccaaca
    52981 aggtaaccgc ctgtgccgca ttgcgcgaat cggtttcttt gcgtaagacc accgtgcctt
    53041 tggcaatgga gtcttccacg cgccccacta tcaggcgggc attgagtttt gccgcctgcg
    53101 cgtcgcggaa agcaatcact tcggggtagt tgtgtcccgt gtcgatatgc accagcggga
    53161 agggcagttt caccggccgg ctgcccagcc ggaaggcttt gcaggcgagg gcgagcagga
    53221 ccacggaatc tttgccgccg gaaaagagca gggcggggtt ttcgcattct gccgccactt
    53281 cgcggataat gtggatggat tcggattcca accagtcgag ttggacgttg ttcggttcgg
    53341 ttttcgtcat accatattcc ttatttcttc tgtttgatcc ttatgaatta tttgtgcagc
    53401 ccgcattctt tgctgttttt atcttcccac caccaccgcc cggcgcggat gtcttcgccg
    53461 gccttgacgg ggcgggtgca ggggtcgcag cctatactgg gaaagccttg ccggtacaaa
    53521 tcgttgtaag gcacattgtt ggcaaggatg tatgcccaca cgtcgtgttc cgaccagtcg
    53581 aaaatcgggt tgtatttatc gatgccgcgc ccggcatcat attcggcaaa cggcagttcc
    53641 gtgcgtgtgg cggactgttc gcggcgttgc ccggtaagcc aagcatccgc cccggcaatg
    53701 gcgcggtcga gcggttcggt ttttcggatg cggcagcatt cgcggcgcgc ttcaacgctg
    53761 tcgtaaaagg caaacctgcc tttgctttcc acataatgca gggcatcttc ccgtatcggt
    53821 tggaaacgct tgatttgcat atgcggataa acacgttcaa tcctatccaa caggtttaaa
    53881 gtttccgcgt gcagcagccc cgtatccaaa gtaaaaatac cgatattgag gttttctccg
    53941 gcgataaggt cggtaattac catatcttcc gcagcaaggc tgctggcaaa ccgtgcatcc
    54001 cggtggctgc cggcaatccg gtgcaggcgt tgtttgaggg tttccgtttt ttccgccaaa
    54061 gccgtttcac tgccattttc aatcggcgga attttccata aagcaggttt gaaaattttc
    54121 attttgtccg ccttgcttta tgcggcttgt tgttcttgtc cggtgcccag tccgtccaat
    54181 gcaaaatacg cgtgttcggc cggattgctt tcttttttgg cgttttcgcc gaaccaagcc
    54241 agtttttcgt gcagccccac cacttcgcct atgacaatca atgccggatt cggcgcggtt
    54301 tcggcgagtt cggcaaggtt ggcgagcgtg ccggttgcgg ttttttgagc cggcagcgtg
    54361 ccttggctga tgacggctgc cggcgtgtcg ggcgagcgtc cgtgctgttg cagccgttcg
    54421 gcaatcaggg cggctttgag cgcacccata taaatcacca aggtctggcg gctgcgggca
    54481 agggtctgcc attcgatgtc gggcgcatcc gccttgcggt ggccggttac gaaaaccgca
    54541 ctttgggcat aatcgcggtg cgtgagcggg atgccggcat aggcggtcgc gccgacggcg
    54601 gcggtaatgc cggggacgac cgaaaacgga atctgatggc gtgccaaggt ttccaattct
    54661 tcgccgccgc gtccgaacac gaaagggtct ccgcctttca aacgcaccac gcgcctgcct
    54721 tcgcgggcca gcctgaccat aagcgcattg gtgtcctctt gcggggtgcg gccgccccgg
    54781 gcgcgcttgc cgacaaaaat ccgttccgca tcgcggcgga cgagggacag tatgccgtct
    54841 gaaaccagcg cgtcgtaaag caccacgtct gcctgctgga tttcctgcag ccctttgagc
    54901 gtcagcagcc ccgcatcgcc gggacccgca ccgaccaaag acacaaaacc gccttgataa
    54961 ttttgttcca actgctttgc caattcccgt tcggcaaggg tgttttgccg gtttttgacg
    55021 agggcggcga aacgtccgtt aaactgcttt tcccaaaagc ggcggcgttc ggtaacggat
    55081 ttcagtttgc ccttgacggc atcgcgccac cttcctgaaa tttccgccat atcgcccaaa
    55141 gacggcggca gcagggcttc cagcctttca cgcagcagtc gggcgaggac gggcgcgctg
    55201 ccggagctgg aaacggcaat ctgaaccggg ttgcggtcga taaccgatgg gaagatgaag
    55261 ctgcaatggt cgcggtcatc caccacgttg accggctttt ggcagctttc ggcaagatgg
    55321 aaaacgcgcc ggttgagggc ttggtcgctg cttgccgcaa tgatgaggaa aaccgtgcgg
    55381 atgtgttcgg cacgaaattc ttcggcaagc cacaggattt tgttttccgc cgccaacgcg
    55441 gagagttcgg cattcaggtg ttttgcggca accctgacct ctgcgcccgc cttcagcagc
    55501 aggctgattt tgcgtgcggc gactgcgccg ccgcctacga ccaatacggg gcggccggcg
    55561 aggttggcga aaatggggaa ataattcact ggctgactcc tttgctgttt gcccgcacct
    55621 tgttgccgat acggtgcgtc gcggcatttt tgtcggaatg cgggtcattt tagacaaaag
    55681 gattttcccg gttaaataat aaaaaggcat ttgttagaag ctgaaagcta tatgggggcg
    55741 gctgcggatg cggcggtttt ccgttttata acggtttggg acggaaaaac ggcctgaagc
    55801 cgtttcgggc attcagaccg tttgcgcgga gggggaatgc cgtccgaagg gcgaaaaggg
    55861 cttcagacgg cattgatgtc gggtttcagg acaggagcag gatggcggct gcggcaagcg
    55921 cggcaaccga taatgcggcg gcaagcgcgg ctttgccggc aaagcggatt gaggttttgc
    55981 cttcgatgta tttgaagccg gttatcatcg ggaggacgag gtttttcttt ttgaacacgc
    56041 ggtatgcggc gacggcggcg atgtggactg cggaaaaaac ggcgagcagt ttgaaaaagt
    56101 tgagatggat tttccgcata aggctgcccg tatgttcgga aaccaaatgg ttgaggtagc
    56161 cgttggtgct gaaggtgttt tcatcggcgg caaaaagccc cgtgccgact tggaacgaca
    56221 cggcggccaa aagcgcaacg accatcagtg cgcccaaggg gttgtgtccg ggttggacgt
    56281 gttcgggaat accgtttttc atatactctc tgatacccga ccatccgcgg acgaaacggg
    56341 agaaacgggc ggtatcgctg ccccaaatgc cccagcagag gcggaatacg agcaggaaaa
    56401 ggataaacag cccgacgcgc gtgtgccatt gcagcatatc gccgccggtt ttcgcgctat
    56461 accacataaa gggtagggat gcggcaagca gccagtggaa aaggcgggtg gggaagtccc
    56521 agactttggt tttgtttttc ataatcggtt tccggcggta gaatcggttt gttttcgagc
    56581 cttaatttta accgattgga ggggaaatgt ttcccgtttt ccatctttca ggcgaaagcc
    56641 gccgccggat gcttcaggcg gcattgcgtt ttcctcatgt tttcaaagcc cgtgcggaag
    56701 attcgcacaa agggactttc ggcacgctcg ccgtagtcgg cggcgcggtc ggcatgagcg
    56761 gcgcgcccgt attggcggca tcggcggcaa tgtatctcgg ctgcggcaaa gtgtgggcgg
    56821 gcttcaatca ggaaacgctt ccgtttgccg tcattgccgg ttttcccgaa attatgttgg
    56881 acacggcaga cggtttgacc aagcgtcaag gcataaacgc ttggacggcg ggttgcggct
    56941 tgggtacgga taaggcggcg gtcggaacgc ttgccggaat tttgacggaa cacacggaca
    57001 agcccgtcct tttggatgcg gacgcgctga acatattatc aaccgatgcc gaaacccgaa
    57061 atctggcacg cgggtgtaaa aacctgattt taacgccaca ccccgccgaa gccgcgcgcc
    57121 tgcttggaac gacggttgca caggttcagg cggatcggac ggcggcagtg aggaagatag
    57181 gggcaatttt cggcgcaacc gtggttttaa aggggcacaa aacattggtt gcctcacccg
    57241 atacggaaat ctatgtcaac gaaagcggca acgcgggatt ggcaacggcg ggcagtggcg
    57301 acgtattggg cggcatcatc ggcagtctgc tcgcacaggg cgtgccggtt tttgaagccg
    57361 cctgcgcggg cgcgtggctg cacggcgcgg cggcggatgt cataaaagaa tcggcaggca
    57421 ttgcggcagg gctgttggct ggcgaaatcg ccccggcggc aaggtggctg cgcaaccgga
    57481 taactgaaag tatgtaagaa gatatagtgg attaacaaaa accagtac
//
LOCUS       DAWFKN010000003        56909 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFKN010000003 DAWFKN010000000
VERSION     DAWFKN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.003"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WGR46_000166"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WGR46_000166"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0855195.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WGR46_000167"
     CDS             complement(1334..3490)
                     /locus_tag="WGR46_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0855196.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGVNIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WGR46_000168"
     CDS             complement(3583..3789)
                     /locus_tag="WGR46_000168"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0855197.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGR46_000169"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGR46_000169"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0855198.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WGR46_000170"
     CDS             4593..5192
                     /locus_tag="WGR46_000170"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0855199.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKINIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WGR46_000171"
     CDS             complement(5238..6026)
                     /locus_tag="WGR46_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0855200.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDTVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVRALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WGR46_000172"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WGR46_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0855201.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WGR46_000173"
     CDS             7840..8316
                     /locus_tag="WGR46_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0855202.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8921..9307)
                     /locus_tag="WGR46_000174"
     CDS             complement(8921..9307)
                     /locus_tag="WGR46_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0855203.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9304..9552)
                     /locus_tag="WGR46_000175"
     CDS             complement(9304..9552)
                     /locus_tag="WGR46_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0855204.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            complement(9922..10245)
                     /locus_tag="WGR46_000176"
     CDS             complement(9922..10245)
                     /locus_tag="WGR46_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0855205.1"
                     /translation="MITRPAQSDLVRIYNFLNDLGAERQANTVMRLLKNSFVSTQSSP
                     SNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFRENAYHL
                     SFPFL"
     gene            10243..10350
                     /locus_tag="WGR46_000177"
                     /pseudo
     CDS             10243..10350
                     /locus_tag="WGR46_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10356..10643)
                     /locus_tag="WGR46_000178"
     CDS             complement(10356..10643)
                     /locus_tag="WGR46_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855206.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVEGLGGDESKDMPECRK"
     gene            10707..10835
                     /locus_tag="WGR46_000179"
                     /pseudo
     CDS             10707..10835
                     /locus_tag="WGR46_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(11025..12383)
                     /locus_tag="WGR46_000180"
     CDS             complement(11025..12383)
                     /locus_tag="WGR46_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0855207.1"
                     /translation="MTIPFKPSLLAMASILALSACISGGGGTPVVKSSETPSNKVTPP
                     ADTVSPAPGADTAGTASGGDGRESKTEIPPKDTQGEGETAQAGADGGVQNDAPQHVET
                     STESTPEGTVESESTPKEAGEEIKPAPKEDTSEGKEAKETDPTQPSSTSESQDGTEEN
                     AVSEDTGMQASPPSETTEHTEPAMNAEDANPPSKAENSDEVGQGGMQADEASGRQDAE
                     EGLQGDDPTAHPPQDQTPQSAADNQPKTGVFGEKYFDGTGEITALVIGGETIDLTKAD
                     EQVSRRNHPSLMSDFDKVMVSQNQYKHLIYGAAKLKDGSYTLFVQGKPTEQLPVGNAT
                     YNGKVLHFRKAYENNWFNDSAYQAAGTFTANVDFSQRTVTGKIDTKDPSGMGMQEFTA
                     SIDGSGFQGKWKSDNDQGDISGKFYGQDGAEMAGRYDYKNQDNRKDGFGVFAGKKEAD
                     AR"
     gene            complement(12515..13144)
                     /locus_tag="WGR46_000181"
     CDS             complement(12515..13144)
                     /locus_tag="WGR46_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0855208.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVEPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            13443..14777
                     /locus_tag="WGR46_000182"
     CDS             13443..14777
                     /locus_tag="WGR46_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0855209.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAATSLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            14770..16431
                     /gene="pqiB"
                     /locus_tag="WGR46_000183"
     CDS             14770..16431
                     /gene="pqiB"
                     /locus_tag="WGR46_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0855210.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            16431..16949
                     /locus_tag="WGR46_000184"
     CDS             16431..16949
                     /locus_tag="WGR46_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0855211.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            16953..17504
                     /locus_tag="WGR46_000185"
     CDS             16953..17504
                     /locus_tag="WGR46_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0855212.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            17494..18135
                     /locus_tag="WGR46_000186"
     CDS             17494..18135
                     /locus_tag="WGR46_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0855213.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSSVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(18128..18358)
                     /locus_tag="WGR46_000187"
     CDS             complement(18128..18358)
                     /locus_tag="WGR46_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0855214.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADHLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            18306..18665
                     /locus_tag="WGR46_000188"
     CDS             18306..18665
                     /locus_tag="WGR46_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855215.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            18696..21548
                     /gene="gcvP"
                     /locus_tag="WGR46_000189"
     CDS             18696..21548
                     /gene="gcvP"
                     /locus_tag="WGR46_000189"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0855216.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(22013..22852)
                     /locus_tag="WGR46_000190"
     CDS             complement(22013..22852)
                     /locus_tag="WGR46_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0855217.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            23137..23212
                     /locus_tag="WGR46_000191"
     tRNA            23137..23212
                     /locus_tag="WGR46_000191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23170..23172,aa:Arg,seq:ccg)
     gene            complement(23277..24470)
                     /locus_tag="WGR46_000192"
     CDS             complement(23277..24470)
                     /locus_tag="WGR46_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0855218.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(24491..25579)
                     /gene="trmA"
                     /locus_tag="WGR46_000193"
     CDS             complement(24491..25579)
                     /gene="trmA"
                     /locus_tag="WGR46_000193"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0855219.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDTSVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGGFTQPNAAVCQKMLEWACGAAEGLGGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFARLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     VVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            25726..26826
                     /gene="aroC"
                     /locus_tag="WGR46_000194"
     CDS             25726..26826
                     /gene="aroC"
                     /locus_tag="WGR46_000194"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0855220.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            26918..27322
                     /locus_tag="WGR46_000195"
     CDS             26918..27322
                     /locus_tag="WGR46_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0855221.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(27396..29381)
                     /gene="parE"
                     /locus_tag="WGR46_000196"
     CDS             complement(27396..29381)
                     /gene="parE"
                     /locus_tag="WGR46_000196"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0855222.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGIEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(29448..29972)
                     /locus_tag="WGR46_000197"
     CDS             complement(29448..29972)
                     /locus_tag="WGR46_000197"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0855223.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            30137..31432
                     /gene="serS"
                     /locus_tag="WGR46_000198"
     CDS             30137..31432
                     /gene="serS"
                     /locus_tag="WGR46_000198"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0855224.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSILDEGGLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(31510..32508)
                     /locus_tag="WGR46_000199"
     CDS             complement(31510..32508)
                     /locus_tag="WGR46_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0855225.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(32633..33709)
                     /gene="prfA"
                     /locus_tag="WGR46_000200"
     CDS             complement(32633..33709)
                     /gene="prfA"
                     /locus_tag="WGR46_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0855226.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(33799..34569)
                     /locus_tag="WGR46_000201"
     CDS             complement(33799..34569)
                     /locus_tag="WGR46_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0855227.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(34684..35673)
                     /locus_tag="WGR46_000202"
     CDS             complement(34684..35673)
                     /locus_tag="WGR46_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0855228.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELVRLKL"
     gene            complement(35740..36423)
                     /locus_tag="WGR46_000203"
     CDS             complement(35740..36423)
                     /locus_tag="WGR46_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0855229.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(36612..37946)
                     /gene="glmM"
                     /locus_tag="WGR46_000204"
     CDS             complement(36612..37946)
                     /gene="glmM"
                     /locus_tag="WGR46_000204"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0855230.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(38076..38933)
                     /gene="folP"
                     /locus_tag="WGR46_000205"
     CDS             complement(38076..38933)
                     /gene="folP"
                     /locus_tag="WGR46_000205"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0855231.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(39073..39966)
                     /locus_tag="WGR46_000206"
     CDS             complement(39073..39966)
                     /locus_tag="WGR46_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0855232.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            40057..42171
                     /locus_tag="WGR46_000207"
     CDS             40057..42171
                     /locus_tag="WGR46_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0855233.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(42236..43714)
                     /gene="ubiD"
                     /locus_tag="WGR46_000208"
     CDS             complement(42236..43714)
                     /gene="ubiD"
                     /locus_tag="WGR46_000208"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0855234.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGSLDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(43734..44249)
                     /locus_tag="WGR46_000209"
     CDS             complement(43734..44249)
                     /locus_tag="WGR46_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0855235.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNWLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(44256..44786)
                     /locus_tag="WGR46_000210"
     CDS             complement(44256..44786)
                     /locus_tag="WGR46_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855236.1"
                     /translation="MVRQGEATLYWFLLIHYHYGREAGWFVWLAAAMCGLWLLRALTA
                     KLPQQRYAALFITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLA
                     RLQQPDLPEKAVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMG
                     MPFAGEWLYRKLVLKV"
     gene            complement(44989..45558)
                     /locus_tag="WGR46_000211"
                     /pseudo
     CDS             complement(44989..45558)
                     /locus_tag="WGR46_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            45598..46208
                     /locus_tag="WGR46_000212"
                     /pseudo
     CDS             45598..46208
                     /locus_tag="WGR46_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            46153..47289
                     /locus_tag="WGR46_000213"
     CDS             46153..47289
                     /locus_tag="WGR46_000213"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ0855237.1"
                     /translation="MDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVR
                     MMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     PSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLW
                     DGQNDPDLPAINLTGSGSRWETEKRIAASSSFAFGGSNCVLIIG"
     gene            47343..47418
                     /locus_tag="WGR46_000214"
     tRNA            47343..47418
                     /locus_tag="WGR46_000214"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47376..47378,aa:Ile,seq:cat)
     gene            47493..48251
                     /locus_tag="WGR46_000215"
     CDS             47493..48251
                     /locus_tag="WGR46_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0855238.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            48252..49316
                     /locus_tag="WGR46_000216"
     CDS             48252..49316
                     /locus_tag="WGR46_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0855239.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            49550..50887
                     /locus_tag="WGR46_000217"
     CDS             49550..50887
                     /locus_tag="WGR46_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0855240.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
     gene            complement(51122..51457)
                     /locus_tag="WGR46_000218"
                     /pseudo
     CDS             complement(51122..51457)
                     /locus_tag="WGR46_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(51438..51719)
                     /locus_tag="WGR46_000219"
     CDS             complement(51438..51719)
                     /locus_tag="WGR46_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEZ0855241.1"
                     /translation="MGRHFGRRRFLTAAAVAAAGAAVSFLPNPFAADDEKRNGDEKRS
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(51728..52522)
                     /locus_tag="WGR46_000220"
     CDS             complement(51728..52522)
                     /locus_tag="WGR46_000220"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0855242.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            52906..54240
                     /gene="gdhA"
                     /locus_tag="WGR46_000221"
     CDS             52906..54240
                     /gene="gdhA"
                     /locus_tag="WGR46_000221"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0855243.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            54382..54756
                     /locus_tag="WGR46_000222"
     CDS             54382..54756
                     /locus_tag="WGR46_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855244.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            54800..55057
                     /locus_tag="WGR46_000223"
     CDS             54800..55057
                     /locus_tag="WGR46_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0855245.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
     gene            complement(55243..56022)
                     /locus_tag="WGR46_000224"
     CDS             complement(55243..56022)
                     /locus_tag="WGR46_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0855246.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            56453..56752
                     /locus_tag="WGR46_000225"
     CDS             56453..56752
                     /locus_tag="WGR46_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0855247.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            56749..>56909
                     /locus_tag="WGR46_000226"
     CDS             56749..>56909
                     /locus_tag="WGR46_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ0855248.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATV"
BASE COUNT        13679 a        15690 c        14082 g        13458 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tcctcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggta tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccagacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gaactgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacgcaaaaa ttcaaaaaag ataaaaatgc
      961 caaacggtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtaatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcaatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa agcgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttggatg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcccgac acggtagttc tgcccattca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatac
     1861 cgacgttgta cagcacttcc tcaaatgaag tctgtttgtc gttgagatcg gcaaggtatt
     1921 tttctttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg cctgacacgg cgaaattcaa ccatgcagga ttgggtttgg
     2101 catgttcgga tgtaataatt tcgacggaat cgccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taaggaccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cacacccacc agccgctggc cgaatgcgcg caacactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagtgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcattgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagaccgtc caccatctcg gcaatcgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acacagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caattggatg cgtaatgtat ggttcgccac ttttccgggt ttgtccgtca tgagcgtgaa
     3361 atgcataggc aacggccttc tcgagctcaa cctgttcttc aggcttgaga taggaagcgg
     3421 tacggaaaag caaggtgcgt gcttcggcag ttaaatggtc gtaaggtgcg gtgggttgag
     3481 ggacaggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttgag acgcgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacag cttcaggcag cgcatctcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaatcacgt cataacctgc cgccgccaac gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga atacgtctgc gtattccaaa aaagcctcct
     4261 gtgcgataag cgactcaaac tcttctttgg aaacaaagtg ataatgtacg ccgtttgctt
     4321 cgccttcacg cggcgggcgc gtcgtgtgcg acacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaatggaca cccagtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gtgcggaata cttccgcctt ttggtcgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcaacatcgt tgccggtttg gacgcacgcg gcttcattat cggcgcggca ctcgcctacc
     4861 agctcaatgt cggtttcgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acgatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagccgcc gccattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggat gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc tggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttcagacagc atttccacat cgttcaaacc gtcgccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcacgcacca cgcttctgat gccgtctgtt
     5461 ttcgacattc ccgcaggcag cagatcgacc gcttcctcgt gccagcgcac cgttttcaag
     5521 ccttcccgtt ccacaatatc cgaccagggc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgctgcaagg cgcggcacac gcattccgac agcgcggaca cggcgatccc ctctccgccg
     5701 acaaacgcat aatccatacc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgcg cacagttttt ccgtgcagca gcgcaaactg cccgtttatc
     5821 gttaccacgg tatccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatgcctt tgccgcgcaa agccgccacc
     5941 gccgttttca cggaaggacg caaggtatcc gtatatttgc ggtacagcgt atcgtcaata
     6001 tcgaaaaaca cgattttggg attcatcata ttctctctcg cactcaaact gccgcattgt
     6061 atcccaatca ggcaaatact tgataaatcc ttataaattt tccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcacac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cggaaagggt tacacccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactttt attaccggca ggcggtctcg aacgtatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaat aacgaatttg
     6481 ccaaactcga cgttctcgag caaggcatta aagaagcgca tgatcgcaac aagaaattct
     6541 tccttaccgt caatacgctg ccgcacaact ccaaactcaa aaccttcgtt tccgatatgg
     6601 agcccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggc ttgattatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcatcca cggcgcattg tgcatcgcct actcaggccg ctgcctgttg tcgggctatt
     6901 tcaaccaccg cgaccccaat caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagt gatgcaggcg atgcccagct tctgcaaggt tttaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaagtcttc ctgattgaag agtccaaccg cccgggcgaa atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaattccaa agaccttcgc ggcattgaag
     7201 tggttgaaaa actcgccaaa atcggcgtgg acagtctcaa agtcgaaggc cgcaccaagt
     7261 cgctttatta tgttgcacgt gtcgcccagt cctaccgcaa agcgattgat gatgccgttg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggcctcgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaaa cccaagacta tcaaaactac ctgaccggcc
     7441 actctattgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac gaaaacggct
     7501 gggcgaccat cgaagtcaaa aaccgcttcg ccgtcggcga ttcactcgaa atcatccacc
     7561 cgggcggcaa ccaaaccatc aaattggaac aaatggcccg caaaggccag cctgtcgatg
     7621 tcgcacccgg caacggcatt caggtcaaaa tccccgatat gcagggcaaa gaaaaagccc
     7681 tcatcgcacg cgtattgaac ccctgagcca caatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc acagcaaaca aggacatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgctggcagc ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccgaca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgctgcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accgattgca aacggcagga atctggttac acggcaagca
     8101 gcaaacagcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc cggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcaaccgct gcaaggagga caatacgact gtcaggcagg ctcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcgacca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag tcgcagacag tacagatagt
     8521 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     8581 gaggcaacgc cgtactggtt tttgttaatc cactataaaa cacagccata tatattaagt
     8641 acctgctctt tatgcgcttt tttaggaaag gaagatcaaa cggttcaata gcgaagtctt
     8701 gcccaaattt gatggacagg atatagaata catgcgtgca taccacaccc tgccaaagtt
     8761 gggagatgca cttaaggttg cacttgtggt cgccaactat gcctatacac gcggatttcc
     8821 cgcgtttata ggccgcgacg gaagtctggc ttttaagcga agacaataac cgaaaagacc
     8881 gcctcggcgg tctttcttgt gtacacagtt tttatatttg tcagcttgat gcattgacaa
     8941 ctctaattcc atattgcgga atatattcat cgacagtcat cagttcaaag ccttccgctt
     9001 gggtttgtgc aatcaacatc ctatcgaaag ggtctttgtg tatttccgga aggcttccag
     9061 cctgttttgc atgaaacaga cctataggca gcatttcaaa atcctcttct tgaagcacat
     9121 caaaaaactc ttccggtaat ttcaacaacc ccttgttctg cttgatggaa atttcccaaa
     9181 tacttgctgc actgacaaag atcgcatttc tcggattttc tatcagtttg cgtgcagata
     9241 tccccagttt cttgtcatcc aacaaccacc acagcaaagc atgggtatca agcagaatct
     9301 ttctcacaga gccgactcct caaaaaataa agctgccgtt tcattgtcat cctcaagaat
     9361 acgtgaaata tccgtatttt ccatatgact gaattttttc aaccttcctg catttcgtgc
     9421 cggtttttca ataccggtta gttggacgca aggcttacct gccttcgcaa taataacgat
     9481 ttcccctgct tctgctcttt gaatcaattg actcaaattg gtttttgcct gatgaatatt
     9541 tgcttgaaac ataacacttc tcatgattag ctaacttgac taatatacat cattaccaag
     9601 attttgggaa tctcattaca tatatttgat tatatccgcc gttttattca caccttgcta
     9661 tttagtaaca atcttcggca accaaaaatg ccgtctgaaa catttttcag acggcatttt
     9721 taatcccctt gcctgattgt gcggcagatt caaatcgggg cactccatac ttccatatct
     9781 gcaccgttca aacatcccgc ctgcggaacg gaatcaaagc ggcagtccgg ttatgttctt
     9841 gaaggcggtt tatccgaacg tgccgcaata aagccgaaac gcattgcccc gcacaaggga
     9901 aagccgccaa accaaatgac gttataaaaa aggaaagctc aggtggtagg cattttcacg
     9961 gaaatgcctc accgtcaaaa tcaatatttg cttacctgat ttatcgtgcc aaaacaaata
    10021 gctataaccg cttttgccat agggcacgat tacttccctt acattttgca aagtttcccc
    10081 cgccaccgac aaatcataag gtttcccatt actcgggctg ctttgtgtag aaacaaaaga
    10141 atttttaagc agccgcatca ctgtatttgc ctgacgttcc gcacccaaat cattcaaaaa
    10201 attataaatc ctgaccaaat cagattgagc ggggcgtgta attatagtgg attaaattta
    10261 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg
    10321 ctgtactggt ttaaatttaa tccactatat caacttcact tgcggcattc gggcatatcc
    10381 ttcgattcat cgccacccaa gccctccacc cagtccgcaa actcattaag cgtaatatgc
    10441 aatcctgttt cccgataatc ctgaaaagcc tgcatcgccc gcttatcgta ggcttgtttg
    10501 gctttcaagc gttctatatg accatccacc gcatccaata cgatactgtg cacactgccc
    10561 ccgaaatccc gggccagctc ttgtaaaacg gctttgcggt atcccagttt gacaccggtt
    10621 acttcctgat tggtaagcat cattattttc ccataaatca aacgtctgac acggcattat
    10681 aaacacaatg cggcatctgc cgccaccttt gcggacgcag tgttaccggc ttccagagct
    10741 acttcgacaa gcagccgctg caaggcggac aatacgattg tcaggcaggc tcgttccacg
    10801 tccgcgtggt tatgcgcgcc aatgtcgtcc gttaacatat cggcaaccaa aaaatgccgt
    10861 ctgaaacatt tttcagacgg catagtccat aaatcaaatc agtaatacag atgatggttc
    10921 aattaggatc gcttctgcag gcaggtcaac acatcaaagt gttgaccgat accctgtccg
    10981 aaggaaaccc gaactgttcc cgactgtgaa acataggata aatatcaacg ggcatctgct
    11041 tcttttttgc ctgcaaatac gccgaagccg tcttttctat tatcttgatt tttatagtcg
    11101 tatctgcctg ccatttccgc gccatcttga ccataaaatt tcccggaaat atcgccttga
    11161 tcattatccg atttccactt accttgaaat ccacttccat cgatacttgc agtaaattct
    11221 tgcatcccca tacctgaagg atctttggta tcaatttttc ctgttacggt tctctgtgag
    11281 aaatcaacat ttgccgtaaa cgttcctgca gcttgatatg cgctatcatt aaaccaattg
    11341 ttttcgtatg ctttcctaaa atgcagcacc tttccattgt atgttgcatt acccaccggc
    11401 aattgttctg tcggcttacc ctgtacgaaa agagtatatg agccatcttt taatttagcc
    11461 gccccataaa tcaggtgctt atattggttt tgagaaacca ttaccttatc aaaatcactc
    11521 atcaaactgg ggtgattcct acgtgatact tgctcatcag ctttcgttaa gtcaatggtt
    11581 tccccaccaa tcaccaacgc cgtgatttct cccgtaccgt caaaatattt ctcgccaaac
    11641 acgcctgttt taggttggtt atcggcagca ctttgggggg tttggtcttg gggaggatgg
    11701 gcggtcgggt cgtcgccttg caacccctct tccgcatcct gcctgccact tgcttcatca
    11761 gcctgcatcc cgccttgccc cacctcatca gaattttcgg ctttcgacgg cgggtttgca
    11821 tcttcggcat tcatcgcagg ctcagtatgt tcggttgttt cagaaggagg acttgcctgc
    11881 atccccgtat cttcgcttac agcgttttct tccgtaccat cttgagattc tgaagttgaa
    11941 gaaggttgtg ttggatcagt ttctttagct tcttttcctt ctgatgtatc ttctttaggt
    12001 gccggtttga tttcttctcc tgcttctttt ggtgttgact ctgattccac tgttccttca
    12061 ggtgttgatt ctgttgaagt ttccacatgt tggggcgcat cattttgcac tccgccatca
    12121 gcgcctgcct gcgcggtttc cccttctccc tgcgtatctt taggcggtat ttctgttttg
    12181 gattctcttc catccccgcc cgaagcagtg cctgcagtat cagcacccgg ggcagggctt
    12241 accgtatcag cggggggggt aactttattt gacggcgttt ctgaggattt gactacaggt
    12301 gtaccccctc cccctgaaat acaggcggaa agggcgagga tggatgccat tgcaagcagg
    12361 cttggtttga aaggaatcgt cataattatc tcctatggca tatttttaat aaaaaatatg
    12421 ataattattt atatctgatg gtttgaaaaa tgccgtcata cgcggcagaa aatacggttc
    12481 cgcttcacga gccgaggcga aaccgtaaaa tatatcaatg cctgtgcggc atcataccct
    12541 cgggggcttc ggcatcggct gccaagccga aagtttcgcg caacacgact ttgtagaatg
    12601 caaaggcttc gcgcgcgcct tggatggctt ccgcttcggc ttcgggagtc aagttcaagg
    12661 cgttcagatg ctcgacgaag gcgcgccagt gtttaccgcg accgtcggga tggggggcga
    12721 ggtggcgcgc gccgtgttcg ccgttgtaat cgagtttttg cgcgtgcttg aacaaaaacg
    12781 ccgcacccaa attagaacct tcggcgcaat aaagccagcc gattgctttg ttgccggttt
    12841 cgtgtggcaa ttctttgtcg aatttgtaag gctcttcgcc caagtctttc aagtcttgcg
    12901 ttacggcatc gtatcgcgcc atgtattcca gctcgggaat ggctttgttt aattcggcat
    12961 ctttatagat gtggtcgacg gccttgtgga aaacggattg gagtttcaaa aatttaatgt
    13021 agttttcttt gctgacaaac ggttcgacag acataacgag attatccacg ctgtcgtgaa
    13081 ccgccgtggt atctgctttc aagcgtttgg caaacgtcag ggcttgattt tcggtttcgc
    13141 tcatagcttt tcctttgtcg gtaagggata aggatgcggt gcgggcaaaa tccgtacctg
    13201 accgcataca aaaataataa acgagattta ttatcacata tttttggaaa aaatatcatt
    13261 tgcgtgatgt ttttaagcag gtattttact attctttaca gaatcgggat tttatcaaat
    13321 gggttcggca gtcggcggac agccgctcaa aaaatatttt tgccggacac caagggtttg
    13381 ttcatactgc cgaacctgcc ggttttgcat cctgattggg tgcgttgctt ttttccttta
    13441 taatgccgcc acttatattt gccactttcc cgatgaagcc gtttgccgaa aatatccccc
    13501 acagccttcg cggcaactgc cgcgacgaag ccctgccgcc gcatacggta gattgcccgg
    13561 aatgcggctg ccgcgcggat gtgccgcaat tggacagggg ggaggcggcg ttctgcccgc
    13621 gctgcggcta taagctcttc agggtgggca ggcatccttt ttccgccccg cccgcttatg
    13681 cggcgacttc gctgattttg atggcgtttg cttacggtat gacgtatatc gaggtcggga
    13741 taccgggtgc ggcatccgtc ctttcgctgc ccgagatgat gcgcctgatg gtgtttcagg
    13801 attatggttt tttggccgaa gtgatgtttg tgctgacctt cggcgcgccg gttctgtttc
    13861 tgctgctgtg cctgtatgtc tatgccgcgc tgatacggaa acaggcgtat cctgcgctgc
    13921 gtttggcaac gcgtgtgatg gtgcgcttga gacaggcgat gatggtggat gtgttttttg
    13981 tttccacttt ggtggcgtat atcaagctct cgtctgtggc agaggttcgc ttcggatcgg
    14041 cgttttatct gatgttcgcg ctgtcggtta tgctgattcg gacttcggta tcggttcccc
    14101 agcattgggt gtattttcaa atcgggcggc tgacggggga taatgcggtt cagacggcat
    14161 cagaaggcaa aacctgttgc agccgctgcc tgtatttccg cgacagtgcc gaatccccct
    14221 gcggcgtgtg cggtgcggaa ctgtaccgcc gacggccgaa aagtctgagt atttcgtcgg
    14281 cgtttctgac ggcggcggtt attttgtatt ttcctgccaa tatcctgccg attatgattt
    14341 catccaatcc tgccgccacg gaggtcaata ccatccttaa cggcatcgct tatatgtggg
    14401 acgagggcga caggctgatt gcggcggtta ttttcagcgc gagtattttg gtgccggtac
    14461 tgaagattgc ggcaatgtcg gttttgattg cgtccgcccg cttcgctttg ccaacgggcg
    14521 caaagaaatt gtcgcacctc taccgcatca ccgaagcagt cggccgctgg tcgatgattg
    14581 atatttttgt gattattatt ttgatgtgtt cgttccacac ttatgccgcg cgcgtcattc
    14641 cgggcagtgc ggcagtctat ttctgcctgg tcgtgattct gacgatgctg tccgcctatt
    14701 atttcgaccc gcgcctgctt tgggacaaac gcgcttcaga cggcattgct ttcaatgaaa
    14761 cggaaaaata tgactgacaa cagccctcct ccaaacggac acgcccaagc acgcgtccgc
    14821 aaaaacaaca ccttgctctc cgccgtctgg ctggtcccgc tgattgcgct gattgccggc
    14881 ggctggcttt gggttaagga aatccgcaac agggggcctg tggttacgct cttgatggac
    14941 agcgcggaag gcattgaggt caacaatacg gtcattaagg tattgagcat cgatgtcgga
    15001 cgcgttaccc gaatcaaact gcgcgacgac caaaaaggcg tggaagtaac cgcccaactc
    15061 aatgcggacg tatccggcct catccgcagc gatacccagt tttgggtggt caagccgcgt
    15121 atcgaccaaa gcggcgtaac cggtttgggt acgctgcttt cgggttcgta catcgctttt
    15181 acacccggca aaagcgacga ggcaaaagac gtgttccaag tgcaggacat tccgcccgtt
    15241 accgccatcg ggcaaagcgg gctgcgcttg aatttgattg gtaaaaacga ccgcatcctc
    15301 aacgtcaaca gccctgtttt gtatgaaaac tttatggtcg ggcaagtcga aagcgcgcat
    15361 ttcgacccgt ccgaccaaag cgtgcattac accatcttca tccaaagccc caacgacaaa
    15421 ctgattcatt ccgccagccg tttctggctg gaaagcggca tcaatatcga aaccacaggc
    15481 agcggcatca aactcaattc cgcccctctg cctgccctgc tgtcgggcgc gatttcattt
    15541 gattcgccga aaaccaaaaa cagtaaaaac gtcaaaagcg aagacagctt cacgctttac
    15601 gacagccgca gcgaagtcgc caacctgcct gacgaccgct cgctgtacta caccgcgttt
    15661 ttcaaacaat ccgtgcgcgg cctgaccgtc ggttcgcccg tcgagtacaa agggctgaat
    15721 gtcggcgtgg tttccgatgt cccttatttc gaccgcaacg acagcctgca tctgtttgaa
    15781 aacggttgga ttcccgtacg catccgcatc gagccttccc gtttggaaat caatgccgac
    15841 gagcaaagca aagaacattg gaaacaacaa tttcagacgg ccttaaacaa aggcctgacc
    15901 gccaccatct ccagcaacaa cctgctgacc ggcagcaaaa tgattgagtt gaacgatcag
    15961 ccttcgtctt cgcccaagct gcgaccgcat accgtttatg caggcgatac cgttatcgcg
    16021 acccagggcg gcggtttgga cgatttgcag gtcaaattgg cggatttgct ggacaagttc
    16081 gacaaactgc ctttagataa gacggttgcc gaattgaacg gttcgcttgc cgagctcaaa
    16141 tccacactca aatctgccaa tgccgcccta agctccatcg acaaactggt cggcaaaccg
    16201 cagacacaaa acattccgaa cgaactgaac caaaccctga aagagttgcg cacaaccctg
    16261 caaggcgtat cgcctcaatc gcctatctac ggcgacgtac aaaatacgct gcaaagtttg
    16321 gacaaaacct taaaagacgt tcaacccgtc attaacactt tgaaagaaaa acccaacgcg
    16381 ctgattttca acagcagcag caaagaccct atcccgaaag gaagccgata atgcgcctct
    16441 tccccatcgc cgccgccctg acgcttgccg cctgcggtac tgtgcaaagc acacaatatt
    16501 tcgtgttgcc cgacagccgc tacatccgcc ctgcaacgca aggcggcgaa accgccgtcg
    16561 aagtccgcct tgccgaaccg ctcaaacgcg gcggactggt ctatcaaacc gacccctacc
    16621 gcctcaacac cgcacaaaac cacgtttggg cggacacctt ggacgatatg ctcgaagcgg
    16681 cgttgagcaa tgcattcaac cgtttggaca gcacacgcat ctttgttcct gcctcacgca
    16741 gcggcagtac cgaaaaatgg acggtctata ttgacgcatt ccaaggcagc tacacgggca
    16801 aaaccctcat cagcggctac gccgtcctac ccgacggtac gaacagaccc ttccatatcg
    16861 aaaccgaaca gcagggtgac ggctacgccg cgatgaccgc cgcactcgaa cagggactga
    16921 aacaggcggc gcaacagatg gtcgagtaaa ccgtgaacta ttgcgaattt gccgcctcac
    16981 ttcccgaaaa caccgacaac ccgaacaaac attaccacga cacgcaatac ggttttccga
    17041 ttgaggacga caatgaattg tttgaacggc tggtgttgga aatcaatcag gcaggattga
    17101 gctggacgct gatgctgaag aagcggcagg cgtttcagac agcatttgaa gggttcgaca
    17161 tcgatacggt tgccgcattt gacgaagccg atattgagcg gctgcttgcc gatgcgggca
    17221 tcgtccgcaa ccgcctgaaa atcgatgccg ccattttcaa tgcacggcaa atccaagcgt
    17281 tgcaacaaga atacggctcg ttcaagaact ggcttgacgc gcaccatccg cgaagcaaag
    17341 acgaatgggt caaactcttt aaaaaacatt tcaaattcgt cggcggcgaa atcgtcggcg
    17401 aatttctgat gagtaccggc tacctcaaag gcgcgcacgc cgaaagctgt ccggtttacc
    17461 gcgaaaccct gaaataccac ccgaaatggc tcgatgccgt ctgaaaaacc aatgaacaga
    17521 agaatcttcc tcctcggcgc aggcgcgttg ctgcttaccg cctgcggcag aaaatccgcc
    17581 cgaacccacg ccaaaattcc cgaaggaagc tccgtacttg ccttgggcga ttcgcttacc
    17641 ttcggctacg gcgcaaaccc tggcgaatcc taccccgcgc aactgcaaaa actgacgggt
    17701 tggaatattg tcaacggcgg cgtatcgggc gatacatccg cccaagccct gtcgcgcctg
    17761 cccgcgctgt tggcacgcaa acccaagctt gtgcttgtcg gcataggcgg caacgacttt
    17821 ctgcgcaaag ttcccaagga gcagacccgc gccaatatcg cgaaaatcat cgaaaccgtg
    17881 cagaaggaaa acatccccgc cgtcctcgtc ggcgtgccgc acatcacact gggcgcgttg
    17941 ttcggacatt tgagcgatca tccgctgtat gaggatttgt ccgaggaata cggcattccg
    18001 ctgttcggcg gcgcgtgggc ggaaattttg ggcgataata atctgaaatc cgaccaaatc
    18061 cacgccaacg gcaaaggcta tcggaaattt gccgaagatt tgaatcaatt tttgagaaaa
    18121 caggggttta gataaacaaa ggtttatccg cacccaagtt gtttatataa tcatgaaccg
    18181 actggaacac caaactgctt cgggacgcat atgccgtctg aagtgcaaaa cctacgccat
    18241 acagccgcat gaagttgcgg cggtatggcg ttttttgaaa aagatggtct gccggttcag
    18301 acggcatgac cgaccgtccg aacctgctgt ggataaagcc cggacaggct gaaatcatgg
    18361 aatattgcga acctgaagaa gcatccgacc cgtacgcaac atacaggcgt gccaacctga
    18421 tggcgggtct gccgctgttt gtcgtgattt tggttctgct caatattgtt tttccgcttc
    18481 cggcgtatcc attggcttgg ctggtgcctg caggtttcat ggttttgggc ggcggctttc
    18541 ccttatcgct gccgcttgtg gcgctgcttg tcctgacctg ctgcattctg gcgcattgtc
    18601 cgccattatc ccgtcttttg tgctgccctt gcccgaatca tccgatgtct aaaaattctg
    18661 cctgatggca gccctacaaa cccgaaggag tagaaatgaa actatctgaa ttgttcaacc
    18721 cgaacgaatt tgccgcgcgc catttgagtt tcggcgacga agcggcgttg cttgccgctg
    18781 tcggcgagaa aagcatggac gaatttgtcg gcaataccgt gccgcaaagc atccgtatgc
    18841 cgtccgaact cgatttgccc gatgccctga ccgaagcgga cgcgttggcg aaattgaaag
    18901 gcattgcgtc gaaaaacgtg atcaacaagt cctatatcgg tttgggttat tacccgaccc
    18961 gcgtgccgaa tgtgattttg cgcaacgtat tggaaaatcc gggctggtat accgcctaca
    19021 cgccgtatca ggcggaaatt gcccagggtc gtttggaagc tttgttgaac ttccaacaag
    19081 tgtgtatcga tttgaccggt ttccctgtgg cgggcgcgtc tttgctggac gaagcgaccg
    19141 ccgccgccga agcgatggcg atggcgcacc gcgtgggcaa ggtgaaatcc gagcgtttct
    19201 ttgtggatgc gcgcgtgtat ccgcaaactt tggacgtgat gaaaacccgc gccaaatatt
    19261 tcggcttcga gctggtggtc agcgattttg ccaaagcgga tgacggcgaa tacttcggcg
    19321 cgctgttcca atacgtcggc aaagacggcg atgtgcaaga cttgcaggat gttatcggtc
    19381 gtctgaaagc aaaaggcacg atcgttgctg ttgccgccga catcatgagc ttggttttac
    19441 tgaaatcacc tgccgaattg ggagcggata ttgctttggg caacacccaa cgtttcggcg
    19501 tgcctatggg cttcggcggg ccgcacgccg cttatttcgc gtttaaagac gcgttcaaac
    19561 gttccgcccc tggccgcatc atcggcgtat ccaaagacgc atcaggcaaa cctgccttgc
    19621 gcatggcgtt gtccacccgc gagcaacaca tccgccgcga aaaagccacg tccaatattt
    19681 gtaccgcaca agcattgctg gcgaatttgg cgggtatgta tgccgtttac cacggccctg
    19741 aaggcgtgaa acgcatcgcc aaccgcattc acgcgctggc ttctgctttt gccgatgcgc
    19801 tggtttcaga cggcattaat gtggttcaca aagtcttctt cgataccgtt accgtcgatt
    19861 tcggcaacaa agagaaagca gaccaagtgt ttgccgctgc tttggaatcg ggctataacc
    19921 tgcgccgcgt taacgatact caagttgcgg ctgcattcca cgaaacatcg gcatgcgaag
    19981 atttggttga tttgtaccgc gcgtttaccg gcaaggatac gttcgcattt gccgatgatg
    20041 tcaaaggccg tctgaacgcc gaactgctgc gtcaagacga cattctgcaa catcccgtgt
    20101 tcaaccgtta ccacaccgaa cacgaaatgc tgcgctacct gaaaaaactc gaagaccgcg
    20161 acttggcgat gaaccgcagc atgatttcat tgggcagctg caccatgaaa ctcaacgcga
    20221 ctgcggaaat gttgccgatt acttgggcgg agttctccga catccatcct tacgctcctg
    20281 aagcgcaaac cgccggctac cgcgaattgc tcgccgatat ggaaaacagc ctgaaatcca
    20341 tcaccggttt tgatgcaatt tccttccagc ccaactccgg cgcgcagggc gaatacagcg
    20401 gtatgcttgc catccgccgc tatcaggaag cgcaaggcga agcgcagcgc aacatctgtc
    20461 tgattcccaa atccgcccac ggcaccaacc ccgccaccgc cgccatgctc ggtttgaaag
    20521 tcgtcgtcgt cgataccgac gaacacggca acgtcaacat cgacgatttg aaagccaaag
    20581 ccgagcaaca ccgcgacgct ttgtccgcca tcatgattac ctatccgtcc acccacggcg
    20641 tgtacgaaga aggcatccgc gacatctgcc gcatcgtcca tgaaaacggc ggacaggttt
    20701 acatggacgg cgccaacctc aacgcccaaa tcggcatcat gcagcctgcc gaagtcggcg
    20761 cggacgtgtt gcacatgaac ctgcacaaaa ccttctgtat ccctcacggc ggcggcggtc
    20821 cgggcatggg tccgattggc ttgaaagccc atctggctcc gttcgcaccg ggacacgctt
    20881 tgaccgacac ccacagcgcc agtgccgacc aaaccgccgt tgctgctgcg gccttcggtt
    20941 ctgcgtccat actgccgatt acttggatgt acctgaccat gatgggcaaa caaggcatgg
    21001 aacaggcgac acgctgggca ttgctcaacg ccaactacgt tgccaaacgc ctgagcgaag
    21061 actatccgat tctgtataca ggcaaaaacg gccgcgtcgc gcatgaatgt atcgtcgatt
    21121 tgcgtccgct caaagccgaa agcggcatca ctgaaaccga catcgccaaa cgcctgatgg
    21181 actacggctt ccacgccccg acggtttcct tccccgttgc cggtacgctg atgatcgagc
    21241 cgaccgagag cgaaagcaaa gccgaactcg accgcttcat cgccgccctg aaacaaatca
    21301 aacaggaagt gctgaaagtc gggcgcggcg aatggccgaa agaggacaat ccgctggtca
    21361 acgctccgca taccgcatcc gatgtaaccg gcgaatgggc gcatccgtac tcccgtgaag
    21421 aagccgtctt tccgttgcca ttcgtccgcg aacacaaatt ctggccgagc gtcaaccgtg
    21481 tggacgacgt gtacggcgac cgcaatctcg tgtgcagctg cccaccgatg gaaaattatg
    21541 aagactgacc gttgaaaccc cgaagacaaa tgccgtctga aacattttca gacggcattt
    21601 tcgtcaacgg cagactggtt gcaccaatac acgtatctcg actataactt taaaacaaat
    21661 gagttaaacc agtatccata catcagcttt tttatcatcc tactttttat tcatccgatc
    21721 gtgcaaacag atttcaaaga tgaaaagcct attcacaccc tttgatgtca tttccacacg
    21781 gacaaacaaa tatagtggat taacaaaaac cagtacagcg ttgcctctcc ttagctcaaa
    21841 gagaacgatt ctctaaggtg ctcaagcacc aagtgaatcg gttccgtact atctgtactg
    21901 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaattc ccataaaaaa
    21961 acggagcaga tacctgcccc gtttttattt aatccgaaat tttaatctaa atttagaatt
    22021 ttgcaccgga ttggtttgcc atatagtcaa cagccgcttt gacttcgtca tcgctcaaac
    22081 cggcattgcc gcctttggcc ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg
    22141 tttctttgcc ttttttgata cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac
    22201 cgggaatcga accgccgtgg cacacctgac aggttgcttc gaagactttt ttaccgtcaa
    22261 cgccgaccgc aggggctgcc gcacccttgt cttctgcctt cgcttctgcc ggagctgcac
    22321 tatcggcagg agcagaagct gttcctgaag cggcattgtc ggcaggcgca gcctcatcag
    22381 gattcgggaa agaaccgccg cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct
    22441 gatcggtcag gtctgccgca ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt
    22501 gttggaacaa ggtatcgaag ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca
    22561 gtttcggagc gttcggcaca ttgctgtccg ccgcgtggca ttggatacag attttgccga
    22621 aaatctgttc gccttggcgt tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga
    22681 caggctggat acgggtctgc gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc
    22741 cgctgcccgc cagcttaatc aggaaataaa ggactgcaat aacaataacg ataccgctca
    22801 caagggtaaa cagtgcagag ccttgggctt tgttgtcgcg gagttgtttc atttggtagg
    22861 cctcgccgtc aggttaggtt gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac
    22921 aatattttgc tggattatac tgaattcaca gggtctttcc aatcgctatc attgaaaata
    22981 tgaaaaaatt tgccaacggt atctgtataa aacaaataat cctttgaaaa taatggttta
    23041 tcctcaagaa aactctcctt atgccgccat acgccgcctg ccggcgcaag ataacctttg
    23101 ccaatttgca gaatttacgt taaccttacg ttttacgcac ccatagctca gttggaagag
    23161 tgtcagtttc cgaagctgga ggtcacaggt tcgatccctg ttgggtgcgc caattataaa
    23221 gagaccgtct gaaagataaa tatttttcag acagtctttt gacttacttc aaactcttat
    23281 ttcaagactt ccgcaaatgc gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg
    23341 acgcacatcc tgccggaatc cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact
    23401 tgttccacgc tcaatcctgt gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa
    23461 tcgcgatcgg ggatttgcgc agttaagaca tcataaagtt tctgccgcat cgcacggatg
    23521 cggtcgcgca tcatataaac ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc
    23581 acgtcggcgg cgatatacgc gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg
    23641 aatttgagct gtccgaacac caaatccgct tcttccttat tcgggcaaac cacgcttaag
    23701 ccgccgacgc gctcgccgta gagcgacagg ttttttgaga aggaattgct gacgaacaag
    23761 ggcaattcca tttccaccgc tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg
    23821 aagccttggt aggcgatgtc cataaacgga atcagtttgc gcgttttgat gatgtgcaac
    23881 acttcgtccc attgttgttc cgacatatcc acgccggtcg ggttgtggca gcagggatgg
    23941 aggatcagga cgctgttttc gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc
    24001 acgccgacag tggcagggtc gtaatatggg taagtgccga cctcgaaacc tgcgccttca
    24061 aaaatgccgc gatggttgtc ccaagtcggg tcgctgacgt aggcgcgcgc ttcaggaaac
    24121 cagcggtgca ggaaatctgc tccgactttg agcgcgcccg agccgcccaa ggtttgtacg
    24181 gtagcgatgc gtccttgcgc gagcgcaggg ttgtctttgc cgaacaataa atgctgcacc
    24241 gcgctgcggt aagtgtccaa gccttccatc ggcaggtagg gcgacggcgc aggcgcggcg
    24301 gcacgggcgg tttcggctcg gctcacggat tccaacacgg gcattttgcc ttcgtcgtcg
    24361 aaataaatgc ctatgctcaa attgactttt tcggggcgcg ggtcgttttt gaaggtttcg
    24421 accaaactca aaatcgggtc gccgggatag tattcgatgt gtcggtacat agtccttacc
    24481 tcttgctttt tcaaaggatt ttctttttca acaatacacc actttcgata tggtgcgtaa
    24541 acgggaattg gtcgaacagg gcggcgcgtt cgaccacatg ggtttccacc aaggtgtcca
    24601 aattggcacg cagcgtttcg ggattgcagg aaatgtagat gatgttgtca aactgcgaca
    24661 ccagcttcaa agtttcctca tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa
    24721 atgcgtaatc cgtcaaagca ataccgccat ccttaaggcg tgcaaactca cgtttgccgg
    24781 tataggcttc ggtaaattcc tcggcagaca gacgggcgat tttgatgttg cctatcccgt
    24841 ttgcctcaat attccattgc gccgcaccta ctgaagtttt ggaaatctcg gttgccaaaa
    24901 cacgttcaaa cttttcagat aaaggcaggg tgaaattgcc gttgccgcaa tacagttcga
    24961 gcaggtcgcc gcccaagcct tccgccgcac cgcatgccca ttcaagcatt ttttgacaca
    25021 cggcggcatt gggttgggta aaaccgcctt caatttgccg ataacgaaaa tcccgattac
    25081 cgacctttaa agtttccgtt acatagtcct gtttcaagac tattttctgc cccctgctcc
    25141 gcccaataac ggaagtatcc aactgttgct gtaacgcttg cgccgcctgc atccactcat
    25201 catcaagcct tttgtggtaa atcatggtaa ccagcatttc cccgctgagc gtagacaaaa
    25261 attcgacggc ataccaacgg tttctcagtt cggcagattg ggcggcaacg gcaatcaatt
    25321 ctggcatcag gcggttgacg gcttcggaag ccgcttcaaa acggtcgcaa cggatcaggc
    25381 ttgccccgcc agccttttgt cctttttcaa acatggcata aaacatttca ccgccttcgt
    25441 gccaaatacg gaactcggca cgcatacggt aatgtttgtc cggagattcg tacacttccc
    25501 actcaggaac atccaaacct gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct
    25561 gctgcgtgta gtcgctcata tcgctatcct gaaaaatcag accggattat aagccgattt
    25621 gcctcgccgc acgcaaactc tttgactgcc gccccttcaa tgacggacgg gcttttgtgc
    25681 taaaatccgc catctttcca cactataccg ataaagggaa aaatcatggc aggcaacact
    25741 ttcggacaac tcttcaccgt taccaccttc ggcgaaagcc acggcgcggg tttgggctgt
    25801 atcatcgacg gctgcccgcc cggcttggaa ttaagcgaag cggatatcca atttgacctc
    25861 gaccgacgca aacccggcac cagccgccac gttacccaac gccgcgaagc cgaccaagtc
    25921 gaaatcctct ccggcgtatt cgaaggcaaa accaccggca cgcccatcgc cctcttaatc
    25981 cgcaataccg accagcgcag caaagactac ggcaacatcg ccaccagctt ccgccccggc
    26041 cacgccgact atacctattg gcacaaatac ggcacgcgcg actaccgggg cggcggcagg
    26101 agttccgccc gcgaaaccgc cgcccgcgtt gccgccggag ccgttgccaa aaaatggttg
    26161 aaagaaaaat tcggcacgga aatcaccgcc tacgttaccc aagtcggcga aaaagaaatc
    26221 cggtttgaag gctgcgaaca catttcccaa aatccttttt ttgccgccaa ccatagccaa
    26281 attgccgagc tggaaaacta tatggacagc gtgcgcaaat ccttggattc cgtcggcgcg
    26341 aagctgcata ttgaagccgc caacgtcccc gtcggcttgg gcgaacccgt attcgaccgc
    26401 ctcgatgccg aaatcgccta cgccatgatg ggcatcaacg ccgtcaaagg cgtggaaatc
    26461 ggcgcaggtt ttgacagcgt aacgcaacgc ggcagcgaac acggcgacga actcaccccg
    26521 caaggcttcc tgtccaacca ctcaggcggc atcctcggcg gcatcagcac cggacaagac
    26581 atccacgtca atatcgccat caaacccacc agctccatcg ccaccccgcg ccgcagtatc
    26641 gacatcaacg gcaaccccat cgaactcgcc acgcacggca ggcacgaccc ctgcgtcggc
    26701 ttgcgcgccg cgccgattgc agaagctatg ctggcattgg ttttaatcga ccacgccctg
    26761 cgccatcgcg cgcaaaatgc ggatgttcag gttaatacgc ccgacattac cctttcaaac
    26821 aaataaaaat ttagccaaaa cacagacttt ataatagaat accgagcatt gccgtgccgc
    26881 tttgacgcac gggtttgttt agaggaatac aaccgaaatg acacaagaaa ccgctttggg
    26941 cgcggcactg aaatccgccg tccaaactat gagcaaaaag aaacagaccg aaatgatcgc
    27001 cgaccacatc tacggcaaat acgatgtatt caaacgcttc aaaccgttgg cgctcggcat
    27061 tgatcaggat ttgattgccg ctttgccgca atacgattcc gcactgattg cacgcgtcct
    27121 cgccaaccac tgccgccgtc cgcgttatct gaaagccttg gcgcgcggag gcaaacgttt
    27181 cgatttgaac aaccgtttca aaggcgaagt tacccccgaa gaacaggcga tcgcgcaaaa
    27241 ccatcctttc gtgcagcagg ctttgcaaca gcagtccgcc caagctgccg ccgaagcagc
    27301 cggatcttcc gcagcagaat aaatccccaa acgaaatgcc gtctgaaaac cgatttggtt
    27361 tcagacggca ttttttcgta tgcggcaatc acggttcaaa tatccaattc cgccgtatcg
    27421 ccttcgcgtt ccatccaagc gcggcgggcg gcggcttcgc ctttgcccat cagtttgacg
    27481 aagatgtcgc gtgtttcgtc atctgcgcct tctgggattt gtacctgcaa cagacggcgg
    27541 gtgtcggggt gcatggtggt gtctttgagt tggtcggggt tcatctcgcc caagccttta
    27601 aaacggctga tggaataagc agtttcttta aagccttctt tttgtagtcg ctccaaaatg
    27661 ctgtcgagtt cgttttggtc gagggcgtag aatttgcggg ccggtttgtt tttgccttgt
    27721 gcattgacat cgacgcggaa cagcggcggc tgggcgacgt agatgtgtcc atcggcgacc
    27781 agtttcggga agtggcggta gaacaaggtc agcagcaaaa cttgaatatg cgagccgtcc
    27841 acgtcggcat cggacaggat ggcgattttg ccgtagcgca ggccgcttaa atcgggatgg
    27901 tcgttaatac cgtgcggatc gacgccgatg gcgacggaaa tgtcgtggat ttcggcgttg
    27961 ccgaagagtt ggtcggggtg gacttcaaag ctgttgagca ctttgccgcg caggggcagg
    28021 atggcttggg tggctttgtc gcgggcgagt ttggctgagc cgccggcgga atcgccttcg
    28081 acgaggaaga gttcgttttc gcggatgtct tcgctttcgc agtcggtcag tttgccgggc
    28141 aggacggcga cgccgctgcc ttttttcttt tcgattttct taaccgaacg catccgcgcc
    28201 tgcgcctgac ggatggcgag ttcggcgatt tttttaccga agtccacgtt ttggttcagc
    28261 cacaattcca aagggtcgcc cgatacggcg gcaacgagtt tcaacgcgtc gcggttggtc
    28321 agcttgtctt tggtttgacc ttggaactgc gggtcgagga cgcgggcgga gaggacgaag
    28381 gcggttttac cgaacacgtc gtcactttgt actttcacgc cgcgcggtag gaggttgtgc
    28441 agattgatga aattgttgac ggcgttgaac acggcttgtt tcaaacctgc ttcgtgcgtg
    28501 ccgcccagcg gggtggggat gaggttgacg tagctttcgt tggcgcacga gccttcttcc
    28561 agccaggtca gggcaaacgc cgctccttcg ccgatgctga aatcgccgtt gtggtcgtct
    28621 gaaatgtagt tttcgcaaga gaagataggc acggcttcct gcgcgtcggc aatcaggtcg
    28681 gtcagatagc ttttcagacc gtcggggtaa tgccaggttt gggtgtgcgc ttcgtcttcg
    28741 cctttgaccg gacgggtcag ggaaacgcgc acgcccggca gcagcacggc tttggcgcgc
    28801 agcaggcgtt cgagttcggg gatgctgtaa ttcgggcttt caaaatattt gccgtccggc
    28861 cagacgcgca cttcgatgcc gctgtctttg acggcgcatt tgcccacttg tgccaacggt
    28921 tcgaccacgt cgccgccggc aaacacgatg cggtggattt tgccttcgcg tttgaccgtt
    28981 acttcaaggc gggtggaaag ggcgttggtg acggatacgc ccacgccgtg caggccgcct
    29041 gaaaaggcat acgcgctgcc gccgtctttt ttgttgaact tgccgcccgc gtgcagacgg
    29101 gtgaatacga gttcgactac ggatacgcct tcttcgggat gcaggccgac gggaatgccg
    29161 cgcccattgt cgtgcacgga aagcgaaccg tcttcatgaa tttgcacgtc gattgcagtc
    29221 gcgaaaccgc ccaacgcttc atccgccgcg ttgtcgatga cttcttggca gatatgggtc
    29281 gggctgtcag tgcgggtgta catgccggga cgttctttga ccggctccaa gcctttgagg
    29341 acggtgatgc tggattcgct gtattggttg tttttagcca taggaataat ctgaaagtaa
    29401 gaaaaacaac gctttcagac ggcctgaaag cgttgcgttc cgttgtttta ccggttgccg
    29461 gaaggttggc gggcggcaaa gtcttcataa ctttccatac cgcgcaggaa gcgggaagag
    29521 agttctttca acgcccccaa ataaacgtcg cgtttgaact cgatgacttc gtcaacgggc
    29581 gcccaatatt gatgccaacg ccagccgtca aattcggggt ggcgggtggc gcgcaggttg
    29641 acatcgcaat cccggccggt caggcgcagg agataccaaa tctgcttctg cccgcgataa
    29701 gagccgcgcc attcgcggcg cacccagttg ttcggcacgt cgtaacgcag ccagtcgcgc
    29761 gtccgcccga cgattttgac gtgttgcggc agaagtccga cttcttcgta aagctcgcgg
    29821 tacatcgcgg tttcggggct ttcgcccggc ttgatgccgc cttgcggaaa ctgccaagaa
    29881 tgttcgcgca cgcgcttacc ccaaaagact tcgtcgcggt tgttggttaa gatgataccg
    29941 acattggggc gatagccttc tctgtccaac acggtgtcgc cctccgttaa attcaatctt
    30001 gggattttcc cacaaatcga acggttttga caaatcagac ggtatggttc aagcatgtcg
    30061 aaacacagac ggactcggga aaatatctta aatttagttt acaataatac gtttcaaatt
    30121 attcgggaat cagaccatgt tagatatcca attgctccgc agcaacaccg ccgccgttgc
    30181 cgaacggctt gcacggcgcg gttatgactt tgataccgca cgttttgacg cactggaaga
    30241 acgacgcaag tccgttcagg tgaaaactga agaactacaa gcctcgcgca acagcatttc
    30301 caaacaaatc ggcgcactga aaggtcaggg caaacacgaa gaagcgcagg cggcgatgga
    30361 tcaagtcgcc caaatcaaaa ccgatttgga acaggctgcc gccgatttgg atgccgttca
    30421 aaaagaattg gacgcatggt tgttgagcat acctaacctg ccgcacgaaa gcgtacctgc
    30481 cggtaaagac gaaaccgaaa acgtcgaagt ccgcaaagtc ggcaccccgc gcgaatttga
    30541 ctttgaaatc aaagaccatg tcgatttggg cgaacctttg ggtttggatt tcgaaggcgg
    30601 cgcaaaactc tccggcgcac gatttaccgt tatgcgcgga caaatcgccc gtctgcaccg
    30661 cgcgctggca caattcatgc tcgatacgca cacactgaaa cacggctaca ccgagcatta
    30721 cacgccctat attgtggacg acaccaccct gcaaggtacg ggccagctgc cgaagtttgc
    30781 ggaagatttg ttccacgtta cccgtggcgg cgacgagagc aaaaccacgc aatatctgat
    30841 tccgacagct gaagttaccc tgaccaatac cgttgcaggc agcattttag acgaaggcgg
    30901 actgccgctg aaactgaccg cccactcgcc ctgcttccgc agcgaggcgg gttcgtacgg
    30961 caaagacacg cgcggtctga ttcgccagca ccagttcgac aaagtggaaa tggttcaaat
    31021 cgttcatccc gaaaaatcat acgaaacgct ggaagaaatg gtcggccatg ccgaaaacat
    31081 cctgaaggct ttggaactgc cctaccgcgt gattaccctg tgtaccggcg acatgggctt
    31141 cggcgcaacc aaaacgtatg acttggaagt ttgggtaccg gcgcaaaaca cctaccgcga
    31201 aatctcaagc tgctccaact gcgaagattt ccaagcccgc cgcatgaagg cgcgtttcaa
    31261 agacgaaaac ggcaaaaacc gcttggtaca tactttgaac ggctccggct tggctgtcgg
    31321 cagaacgctg gtcgccgtat tggaaaacca tcaaaacgcc gacggcagca tcaacatccc
    31381 tgccgcactg caaccgtata tgggcggcgt tgccaagttg gaagtgaaat aagtttgcaa
    31441 gctgcctgaa cgtcaaatgc cgtctgaaac ctggttcaga cggcatttcc tttaaacttt
    31501 taaaacacat caaccgtcgg cacgaaccgc attgccgcaa tcgccggtct gtccgacctc
    31561 gcggatattg gacagcgtaa cttccgaaat attacccaac gcctcttccg tcaaaaatgc
    31621 ctgatggccg gtaaacagca cattatgaca agacgacagg cggcggaaca cgtcgtcggt
    31681 aatcacatcg ttggatttgt cttcaaaaaa cagctcgcgc tcgttctcgt acacatccat
    31741 gcccaatgcg ccgattttcc ggcgtttcaa cgcctcaatc gcggcggcac tgtcaatcag
    31801 cccgccacgg ctggtgttga taatcatcac gccgtctttc attttgtcga acgccgcttc
    31861 gttcagcata tagtggtttt ccggcgtggc ggggcaatgc agcgtgatga tgtccgaccg
    31921 ggcatacagc tcgttcaact ccacatacct gccgccgatt ttttccgctt cggggttgca
    31981 aaacggatcg taagccagca ggttcatgcc gaaacccttt aaaatccgca tcgttgcgat
    32041 accgattttc cccgtgccga taacgcccgc cgttttgccg tacatattga aaccggtcag
    32101 accttccagc gaaaaattcg catcgcgggt acgctgatag gctttgtgga tacggcggtt
    32161 caacgtcagc atcagaccga ccgtatgttc cgcaaccgat tcgggcgaat aggcaggcac
    32221 gcgcacgact ttcaagccca actcttcagc cgctttcaaa tccacattat tgaagccggc
    32281 acaacgcaac gccacagttt tcacgccaat ttgcgccaat ttttccaaca cgggccggct
    32341 gccgtcgtcg tttacaaaaa tacaaaccgc ttccgcgcct tccgccattt tcgccgtttt
    32401 cgcatccagc ataaaatcaa aaaactccag ctcgaagcca aaatgccggt tggcgcgggt
    32461 aaaatgttcg cggtcatagc ttttcgtacc gtaaatcgca atcttcatca atatgtccag
    32521 ttgtcgtcta tggttgagaa acggcataat acactgaatt caaacaaatc agtagaatat
    32581 ggtggattaa aattgattgc atgcacggca tttccatttc aaaacacaaa actcaatcgc
    32641 ccattgccgc caaaagctcc gcctgatgtt cggcaatcag ggcatcggtg atttctgcca
    32701 aatcgccgtc catcacaaaa tccagcttgt gcagggtgag gttgatgcgg tggtctgtta
    32761 ccctgccttg gggatagttg taggtgcgga tgcgttcgct gcggtcgccg ctgccgatga
    32821 gggatttgcg ttcggcggct tctttggctt gggcttcgcg tttttgcgcg tcgttcaggc
    32881 gggcggcgag aactttcatt gcctgcgctt tgttggcgtg ttggctgcgg ccgtcttggc
    32941 attcgaccac catgccggtg ggcaggtggg taatgcggac ggcggaatcg gttttgttga
    33001 tgtgctgacc gcctgcgccg gatgcgcgga aggtgtcgat gcgaaggtcg acggggttca
    33061 actcgatgtc ttccagttcg tccgcttcgg gcatgacggc aacggtgcag gcggaggtgt
    33121 ggatgcggcc ttggctttcg gtggcgggga cgcgctgcac gcggtgtccg cccgattcaa
    33181 atttcaaacg gctgtacgcg cctaagccga taatacgggc gataacttct ttatagccgc
    33241 ccaactcgct ttcgttggcg gacacgattt caacctgcca gcggttgcgt tcggcgtagc
    33301 ggctgtacat acgcagcaaa tcgccagcaa acagcgcggc ttcgtcgccg cccgttccgg
    33361 cgcgtacttc gataaagatg tttttgtcgt cgtcggcatc tttgggcagc agcagttttt
    33421 gcaattcggt atcgagtgcg ccgattttgg ctttcgccgc ttcgatttct tcggcggcaa
    33481 agtctttcat ttcggggtcg gacaacattt cttcggcatc cgccaaatcg ctttgcgcca
    33541 gttgatagtt ttggaatact tcgacgacgg gggtcagttc agcgtgttcg cgcgtgagct
    33601 tgcggtagtt gtccatatcg gacgtggctt cgggttgccc taaaaggtgg gtaacttctt
    33661 ccaaccggtc gccgagttgt tgcagttttt ctaaaataga cggcttcata attcttccat
    33721 aacaaacgcc gcctgaagtt cagacggcat caaaacgcgg tcattataat atattttctg
    33781 aatattcaaa aagataatct agggacggag caggctgtcg gcattgcgtt ccgttatcgc
    33841 ggcgatgtcc gccacgccga caccgcgtat ctgcgcggca atttcggcaa tccaaagaat
    33901 attggcaggc gtattaatct cttttttcag cataaacggg ctgtcggttt ccaatacgaa
    33961 atccccgtcg ttcaaggctt taagcgtatc gcgcacttta cgcgcgttcg gattgagcaa
    34021 caacgaaccg atgccgattt tgaaacccag tttcgtcaac acgcgcgctt cttccgcgct
    34081 gcctgagaag gcgtgaacga tgccgccttg ggcaaagcct gtctgtttga cggcggcggc
    34141 gatgtcggcg gtggctttga gattatggat aatcacgcgg cggcgcaggg tttgcgcgat
    34201 ggcaagctgg cggacgaaaa cttggatttg ccgttcgcgc tgcggcggcg tttgggtttt
    34261 gtcgtaaaaa tccaagccga tttcgccgac ccatgcctgc ggataacgtg ccaacatcgc
    34321 ttccaggcgg acgcaatcac gctcggcaat gccgtctgaa aaccaaggat ggatgccgag
    34381 cgcaatacgg atttgaccgt gttcggacgg catttccgac agttccgcca cgctttgcca
    34441 atcctgcgga caggtggcgg gaacgataaa ccgcttcacc ccaactttcc gcgcggcggt
    34501 caggatgtgc ggcaggtttt cgcgcagggc ggcatcggca aggtggcagt gggtgtcggt
    34561 gaagttcatt tcgattccgc aatgcggcag gttcacataa aaataaggca gggcagcaag
    34621 ggatgccgtg tacgggcggc gcataaggcg cggtcaacgc cccgtcattg ccatttcaat
    34681 ccgctataac ttcagtctta ccaactcttt ttccacctct tccttacccc agccttgcga
    34741 tacggcgagg gtcataagcg cggtggcggt ttcaaggttg catttgccgc cgttgatgat
    34801 gcccgagttg cggaacgcat tgccttgcgc gtaaacggcg gcggttttgc cttgtcggac
    34861 ttggctgatg ttgagcaaca atttgccttg ctgcgtgaaa tcttgcacgg cgcggataaa
    34921 accttcgtct gcgggggtgt tgccgtgtcc gtagctttgc aggataaggg cttgggcggg
    34981 aagctgtccg agtccgtccg caagttcttg gacggcaaag ccggggataa gcgtgcggac
    35041 ggcgattttc gcctgcgggt cgggataacg gattttgagg ccgtctgaaa catctgccgc
    35101 gtcctgattc gggatgcgga cattgtgcca gccctgggtt tcgtcccact ccgccagtgc
    35161 gccgaaatgc ggattgtcga aaccggcggc ggtttcggtg ctgactttgc tgctgccgac
    35221 ggcgggataa agtttgccgt caaaggcaat aagcgtctgc ttcaatccga gtgggaatgc
    35281 ggcaacggca gtcgccagat tgcgcggcgc gtcgctgccc acggcgttgt acggccattg
    35341 ggagccggtc aggacgattg gcttgtccaa accctgcagg gcgagggcga acagattggc
    35401 ggtataggcg agcgtgtccg tgccgtgcag caccaaaacg ccgtcgtact gcggcagcgc
    35461 ggcggcaatg atgtccagcc aagcgcacca gttttgcagt tggacggcgg aagaatcaat
    35521 cagcggccca caaacgtgcc aatcgaaatc catgccgttt gaaaacggcg cgagtgcctg
    35581 cccgaccagc gcggtatcgg ggcgcaggcc ttcgctgctt tgcgtcatgc cgatggtgcc
    35641 gccggtataa aggacaaaaa ttttctgttt catggctcat cctgaaaatg caaagaccgc
    35701 ctgaaacgcg aaatgcgtcc ggacggtcga aaatctgatt tactgctgct tctgtgccgc
    35761 ttttttcgct gccttttccg ccttgcgttt ggcgcgtttt tcgctcaatt gtgcgcggta
    35821 aagctgataa tgtcggcgtt tgcgccacca gaaccacgcc agcgccgccg ccagcacgcc
    35881 caatgcgatg aagatgccgg attgcaggct gtgcattttc gccatcagcc aatcgatgtt
    35941 gtgcgcgccg tactcgccca agtaaatcca aacgggcacg gaaatcagcg cggcaagccc
    36001 gtccataatc agaaagcgca gatacgatac tttgcggctg atgccggcgg taacgaaaac
    36061 ggcagtccgc aaacccggca ggaagcgggc gacaaacaga acccagttgc cgtatttgtc
    36121 gaatttttcc tgaacctgtg cgtaacgttt cggcgtcatg atgcgcgcaa taggtttgaa
    36181 cttgaggatt ttctgccccc agatgcgtcc ggcggcgaac atgatgccgt ccccgaccaa
    36241 tacgccgagc ataccgactg caaacataat atgcggattg gtataaccca tacccgaaat
    36301 cacgccgcct gttaccaagg tcaaatcctc gggaatcggc acgccgaaac cgcagatgac
    36361 caaaacgaaa aacacggccg catagccgta ttcgacaaaa aaggcttcca aaagggcaaa
    36421 catagcggat attccattgt cggagataaa aagtcagaac aagccgaaac attttctgcg
    36481 cgaaacggca ttctatcaaa gattatgccg tctgaaagcg gcaaggggca gattcgcgcc
    36541 ctctgccgga cgttcagacg gcatttaaac aggaaaaccg ccgctgttga aacggttttg
    36601 cctgattttg cctaaaggct gccgatggcg gaagcaatac gttccgcacc gtcgcgcgcc
    36661 caatccgcct gccgcgcctc aaccatcact cgcacgacgg gttcggttcc cgaagcgcgc
    36721 aacacgacac gccctttgcc ttcgagttct ttttccactt ccgccaacac gtcttttgag
    36781 gcttcctgcc atttctgacc tttttggatg cgcacgttaa tcatagtttg cggatacggc
    36841 tgccaatcgg cgcaaacggt ggcgaggtct tggttcagcg tttgcagtgc cgccaaaact
    36901 tgcagcgcgg aaataatgcc gtcgccggtg ttgtgtttgt ccatgcacaa aatatggccg
    36961 ctggcttcgc cgccgatgag ccagccgcgt tggttcagct gttccaacac atagcggtcg
    37021 ccgactttgg cgcggcagaa atcgacgccc tgctctttca gggcgatttc catcgccata
    37081 ttggtcatga ccgtgccgac cacgccgccg atgttgatac cttctcgggc gcgggcttta
    37141 gcaatgacgt aaatcaggct gtcgccgtcg taaaccttac cgtttttatc gaccatcatc
    37201 aggcggtctc cgtcgccgtc taaggcgatg ccgtagtcgg cttcatgctg taaaacggct
    37261 gcctggagtg tcttggtata agtcgcaccg catttttcat tgatattgta gccgttgggt
    37321 tcgtcgccga tgctgacgac ttgtgcgccc agttcgtgaa acaccttggg tgcgacaccg
    37381 taccccgcgc cgttggcagt atcgataacc aacttcaaac cccgaaggtc ggaatggctg
    37441 ggaaaggtag atttgcaaaa ttcgatatag cggtcgtccg caccactgat gcggcgtgcg
    37501 cgaccgaggc gtgcagaagg ctgggttttc atttcttcgt cgattttggc ttcgatttcc
    37561 aactcgattt catcgctcag tttcacgccg ccctcggcaa agaatttgat gccgttgtcg
    37621 gaataggcgt tgtgcgacgc ggaaatcatc acgccggcgg acaggcgcaa cgcgcgggtc
    37681 agataagcca cgccgggcgt gggcagcggt ccggtctgta ccacattcac acccgccgcc
    37741 gtaaaaccgg ccaccaaagc ggcttccagc atataaccgg aaatacgcgt atccttgccg
    37801 attaaaacgg tcggtttctg gtcagtgtcg tgctgaacca aaacctgacc cgccgcatag
    37861 ccgagtttca atacaaaatc gggcgtaatc ggaaactgcc cgacttcgcc gcgcacgccg
    37921 tccgtgccga aatatttttt tgccatgtgt tgctccgaga atgtgaaccg ttgtccgaga
    37981 ttatacagtc agtttgtgcc ttgctgtctg caccgttgat gccgtctgaa accgccccgt
    38041 ccttttcaga cggcatgaag tatgtgaacc gctgtttaca gattgatgcc caacgcttcc
    38101 cacaccttca acgcatccgc tgtcgccttc acatcatgca cccgcacgat ttgcgcgccg
    38161 cgcgctacgg aagccaacgc tgccgccacg ctgccgtgta cgcgttccgc cgcatttgcc
    38221 tcgccggtca gctcgcctat cgtgcttttg cgcgatacgc cgatgagcag cggaaaacct
    38281 gtttccgcca tcaattcggg caaatgccgc atcagcgcga tattgtgttg caagggtttg
    38341 ccgaagccgg agccgaagcc ggggtcgagt atgatgcgtt gcggtgcgat gcctgccgcg
    38401 atacattccg ctgagcgcgc tttcaaatac cgcgctactt caccgacaac atcttgatat
    38461 ttcggattaa tctgcatggt tttgggcaaa ccctgcatgt gcatcaggca aatgcccgtg
    38521 tccgcctgac gcgccagcaa ttcgaccgcg ccctcgtcat tcaacgccgc cacatcatta
    38581 ataatatcga cgccgccgag tgccaacgct ttttccataa tcaccgtgcg gcgcgtgtcc
    38641 aaactgatgg gaacgcccca ccccgccact tccgccaaaa caggctcaac ccgcgcccat
    38701 tcttcttcag gcgaaacata atccgcaccc gaccgcgtcg attcgccgcc gatgtcgaga
    38761 atgtccgcac cctctttcaa aagccgttcg gcgtgcgcca aagctgtttg ggcattttgc
    38821 gaatacgcgc cgccgtcgga aaaagaatcg ggcgtgagat tcacgatacc catgattttc
    38881 ggtttgtcca aaccgatttc aaaccgtcct gcctgccaaa cgtgtcgtgc catctgaact
    38941 cctcccaaaa taaaaaacag attatatgcc gtctgaaacc gtcttgtgcg cttcagacgg
    39001 caccgctatt caggcggcag acggcatgtt gtccgaatgt ctgctccgcc tttgaatctg
    39061 ccggtatgcc tgctatccgc ccgacttttc aaaacaggtt ccgacgattc cgcacgcgcc
    39121 tgccgccttt gccaagccgt acaggatttc ctgcggcata tcgcggttcc ataatcccgt
    39181 aatattcgca atcacgggca gatggctgat ttggcggact ttcacgatgg attcgacatc
    39241 caaacggtag ggatggtctt tggtatggct caacacgccc gactcgccca gaatcaggtg
    39301 gcggttgccg cgcgagacaa catattctgc ggcattcaac caatcttcgg cagaatggtg
    39361 cttgtcttta cacagcacca agggaatgtt caggcttccg gcttcattca atacggcaag
    39421 atcggacatc agcccgccgc ccaaatacag gatgtccgcc cccgcattca aagccgcttc
    39481 gacatggcgg acgttgcgga cgcgcaccaa tacgggtttc cctgcgccat gcgccgatgc
    39541 ggtctgttcc gccaaccgtc tgcaccgtcc tcgcccttca tccgcacttg aagtgtcgta
    39601 taagtttgcc gaagtgaaaa acggatctag aaacactgca tccgcattgc gccatactga
    39661 cggttctgcg gcgatacaga cggtttcccc gccgccgaaa gccacgcctt tggcggcaac
    39721 gcggctgtct tccgcccgat tttcccgact gacggttttc catgtatcca aaatgcggac
    39781 ggctttctcg acctccggca gcgtctgcac ctccctgacg ctcaaaaccc tatcgtcgcc
    39841 gattgcgccg atgacggtac gctcgtcgcc gtgagaaatg tgttctcgca gacctctgct
    39901 gcggataaag gcgacaacgc cggcaatgtc cgcttcggcg gcacgcctgc tcatgacaat
    39961 aatcatattt cctcctgaca caagaaacgg cctgcccaaa ataggatttt tgcaagccgt
    40021 gttatactgt ggcgtgtttt acagattgtt cgggctatgg atttattatc ggttttccac
    40081 aaataccgtc tgaaatatgc ggtggccgtg ctgacgatac tgcttttggc ggcagtcggg
    40141 ctgcacgctt ccgtatatcg caccttcacg cctgaaaaca tccgcagccg cctccaacaa
    40201 agcattgcac acacacaccg gaaaatctcg tttgatgcgg acattcagcg caggctcctg
    40261 ccccggccga ccgtcatcct gaaaaacctg accattaccg aacccggcgg cgaccagact
    40321 gccgtttccg tccaagaaac caaaatcgga ttgagctgga aaaacctgtg gtcggatcag
    40381 atacagattg aaaaatgggt ggtttcgagt gcggaacttg ccctgacgcg cgacgggaaa
    40441 ggtgtttgga acatccaaga cctgatcgac agccaaaaac gccaagcctc agtcaaccgc
    40501 attatcgtcg aaaacagcac cgtccgcctc aatttcctgc aggaacagct tatcctgaag
    40561 gaaatcaacc tcaacctgca atcccccgat tcgtcggggc agccgtttga aagttcgggc
    40621 atactggttt ggggaaagct gtccgtcccg tggaaaagca gggggctgtt cctttcagac
    40681 ggcatcggca cgcccaaaat ctcaccgttc cattttgaag cttccacttc gctggacgga
    40741 cacggcatta ccatttccac caccggcagc ccttctgtcc gcttcaacgc cggcggagcg
    40801 gatgccgccg gcctcggcct gcgtgcagac acttccttcc gcaacctcca cctgaccgcc
    40861 caaatcccta cgctggcact cagaaataac agcattaaaa ttgaaaccgt caacggcgca
    40921 tttaccgccg gcggcgaata tgcccaatgg gacggttcgt tcaaactcga caaagccaac
    40981 cttcactccg gcatcgccaa catcggcaac gccgaaatct ccggcagctt caaaacaccg
    41041 cgccaccaga ccaacttctc cctcaattcg ccgctcgtat ggacggaaaa caaagggctg
    41101 gacgcgccgc gcctgtatgt atcgaccctt caggataccg tcaaccgcct gccgcaaccc
    41161 cgtttcatca gccggctcga cggttcgctg tccgtaccga atctgcaaaa ttggaatgcc
    41221 gaattaaacg gcacattcga ccgccaaacc gttgccgcga aattcagata cacacatgaa
    41281 gacgcaccgc atctggaagc cgccgtcgca ctgcaaaaat tgaacctgac cccctatctt
    41341 gacgacgtgc ggcaacaaaa cggcaaaata tttcccgaca ccctcgccaa gctgtccggc
    41401 gacatcgagg cgcacctgaa aatcggaaaa gtccaacttc ccggcctgca actggacgat
    41461 atggaaacct acctccacgc cgacaaaggc catatcgcgc tcagccgttt caagtcaggg
    41521 ctttacggcg gccataccga aggcggcatc agcatcgcca acacccgtcc cgccacttac
    41581 cgcctgcaac agaatgcaag caacatccaa atccaaccgc tgctgcaaga cctgttcggc
    41641 ttccacagct tcagcggcaa cggcgatgcg gtcatcgacc tgaccgcggg cggcgaaacc
    41701 cgaaaagagc ttatccgctc gcttcagggc agcctgtcgc tgaatatttc caacggcgcg
    41761 tggcacggta tcgacatgga caatatcctg aaaaacggca tttcgggcaa aactgccgac
    41821 aatgccgcac ccagcacacc cttccaccga ttcacgctca acagcgaaat ttcagacggc
    41881 atcagccgcc acatcgatac cgaactcttc tccgacagcc tctatgttac cagcaacggc
    41941 tataccaatc tggatacgca ggaattgtct gaagatgtcc ttatccgcaa cgccgtccat
    42001 ccgaaaaaca aaccgattcc cctgaaaatc accggtacgg tggacaaacc gtccattacc
    42061 gtcgattacg gcaggctgac cggcggcatc aattcgcgca aagagaaaca gaaaatcctc
    42121 gaagacaccc tgctggaaca atggcagtgg ctcaaaccta aagaaccgta aacatcctgc
    42181 gtacaaaaat gccgtctgaa acacccccgc gcttcagacg gcatgaccgt aaaacctaca
    42241 accccaattc ctcccaaatc ccatcaatct tagccgtaac cgcagggtct tttttgatga
    42301 cgcgccccca ttcgcggtcg gtttctcccg gccatttgtt ggtcgcatcc aaacccattt
    42361 tgccgccgag tccgctaacg gggctggcga agtcgagata atcgatgggc gtgttttcta
    42421 ccaaaacagt gtcgcgcacg gggtccatgc gcgtggttac cgcccagatg acttctttcc
    42481 agtcgcgcac gttcacatcg tcatccacca cgatgatgaa tttggtatac ataaactggc
    42541 gcaggaacga ccagcagccc atcatcacgc gcttggcgtg tccggcgtac tgttttttca
    42601 tgccgatcac cgccatgcgg taggagcagc cttcgggcgg caggtaaaaa tcggtgattt
    42661 cggggaactg cttttgcaaa agcggtacga acacttcgtt caacgccacg cccaaaacag
    42721 cgggttcatc gggcggtttg cctgtgtagg tcgaatggta aatcgggttt tcgcgcatgg
    42781 tgatgcgctc gaccgtaaat acagggaaat gatcctgctc gttgtaatag ccggtgtggt
    42841 cgccgtatgg gccttccaac gcggtttcgt tcggatgaat cacgccttcc aatacaattt
    42901 cagcacgggc aggcacttgc aaatcgttgc cgatacattt caccagctcc gtccgcgaac
    42961 cgcgcagcag tccggcaaac tggtattcgc tcaaagtatc gggaacaggc gttaccgctc
    43021 cgagaatagt ggcagggtcg caaccgagca cgacggcgac gggatacggc gtatcgggat
    43081 tgagtttgcg gaactcctga taatccaacg agccgccgcg atgcgacagc caacgcataa
    43141 tcagcttgtt tttgccgatg agttgttggc ggtaaatgcc gagattttgg cgttttttgt
    43201 gcggcccgcg cgttacggtc aagccccatg ttaccagcgg cgcaacgtct tccggccagc
    43261 aatgctgaat cggaagttta tataaatcaa cgtcttcgcc ttcccacacg atttcctgac
    43321 acggcgcgtt tttcaccacg ttcggcgcca tgctccaaat gtctttcaag agcggcagtt
    43381 tggaaaacgc gtctttgatg cctttgggcg gttcgggttc tttcagatac gccagcgtct
    43441 gcccgatttc gcgcagcttg gacacgctgt ccgcgcccat gcccatcgcc acacgttcgg
    43501 gcgtgccaaa caaattcgcc aacacaggat aatcgtagcg cgtaccgtcg ggcttaatcg
    43561 ggttttcaaa caacaacgcc ggcccttcgg cgcgcaacac gcggtcggcg atttcggtca
    43621 tttccaaatg cggggaaaca ggatgcgcga cgcgcttgag tttgccctgt tgctcgagca
    43681 tagcgatgaa gtcgcgcagg tctttgtatt tcatattcat cctttttgtc cttttatcct
    43741 gagcaatccg attcggatac cgcccctatc cttgcctgcg cttcggcata ttctatgccg
    43801 tgataaaagt cgcgtaccag cggatgtccg ccactttgat gaagttgcag caaagggcgt
    43861 tggttgtcgg actgggtaac gacattgcag tgcagaccga aggtgtcggt ttcataaggg
    43921 ggcagccagt tgcagatcat gccgaaataa acgacgtttt ccggattgtc gtgaaagtgg
    43981 ctttgatagt cgttaaaact cttttcactg acagataccc ataccccata ttccagcgtt
    44041 tcctcatgcc cgataatcgg aataagcagc accgcgcgga taaaacggtc ggtttggtcg
    44101 ggatagcgga tgatgcagaa atcagaatca cattccgctt gataagcaat gcgttcttct
    44161 tcactgagtt gattataggg atagggggcg gtaaagccga ttgcgggcat atcttcatgt
    44221 ttttcgccgc aggaagtgca agtgtacata aggtttcaga ctttcaaaac gagtttgcgg
    44281 taaagccatt cgccggcaaa cggcatcccc atcaatacat aggcaatcac gccggtataa
    44341 accgcccacc aatcatatcg ccccaaccgt gccaacaaag cggcaagcgt cccgttgatt
    44401 ataaaaaata cgcaccaaac ctgcgttacc cggcgggtat agcgcacggc tttttcaggc
    44461 aggtcgggct gttgcagccg cgcaagtttt tcaatcaccg tctgcccggc aaacaggctt
    44521 cccccgaaca ccgccaacat catcagattg acgaggacgg gataccaata cattgagtcg
    44581 cgccgcccga acaccaatac tgcggcaaaa aacagtgtaa taaacaaagc cgcataacgc
    44641 tgttggggca atttggcagt cagggcgcgc agcagccaca acccgcacat cgccgccgcc
    44701 agccagacaa accagcccgc ctccctgccg taatgatagt ggattaacaa aaaccagtac
    44761 agcgttgcct cgccttgccg tactatctgc ggcttcgtcg ccttgtcctg atttttgtta
    44821 atccactata ccacaaagcg ggataggcaa tgcttaatac ggtcagaaaa atatgtccga
    44881 aaaaaccggg tttcattttg aatccgcaca aatgttttca gacggcatcc gataaaaaca
    44941 tgccgtctga aaaataatta gaaatacccg attagcccgc ctgaatcttc aataccgcct
    45001 gtaccacgtc gttgacggtg cggacattgc ggaaatcttc agcctgcagc ttgcgggctg
    45061 ccacagccat tcacgattgc gatatacccg gcggcattcc cgactgccgt tcagcagaaa
    45121 gcgcacccat tccaaaccgc tccaatatgc ctcgggcagc ataccggctt cagacggcat
    45181 atcgtccgaa gcagataaag tcaggctgta acgcgtccct ttggtcagaa ccatcgccaa
    45241 agcataagca aacggcgcgc gcgttgccga tacggcatat ccttccggca gcggatcgtc
    45301 cgccgccaaa accaaaaccg acccgcatcc ctcttccaac agtgatgccg cttccgccaa
    45361 tgccgtttcc acaccgtcgg cacacacggc caatgccgtc tgctcgctca tatcccgcct
    45421 caatatcgac cattaccccg ccgttgcatt gtgcaccgac aaaccgaacg aagtcggcga
    45481 cacggtttgc gatttcaaca gttccaacca caaatcgaaa ctgcgcgcca tttccccgtc
    45541 gtgcgaggca taaactatag tggattaaat tttaggggct gtactagatt agcagatatg
    45601 ttaccctcga aatatgaaga taacacactg caaattaaag aaaaaagtac agaaagaact
    45661 gctccgtttt ttgtgctgga agttaccgcc cgttctgccg ccgatatttt gggtatccat
    45721 cccaattcgg cagcactgtt ctaccgtaaa atccgcacgg ttatcaacca tcatttggcc
    45781 ttggctgccg atgaggtttt tgagggccct gtcgagccgg acgaaagcga tttcggcgga
    45841 cggcgtaaag gtagacgtgg tcgcgggcgg caggaaaagt ggttgtcttc ggcattctga
    45901 aacgcaacgg acgggtctat accgttgtcg taaacaatgc caagtctgaa acgttactcc
    45961 ctgtcatcaa aaagaaaatc atgccggaca gtattgttta taccgatagt ctgagcagct
    46021 gcgacaagtt ggacgtgagc ggttttatcc attaccgcat caaccattcc aaggaatttg
    46081 cagaccgtca aaaccacatt aacggcattg agaacttttg gaatcaggca aaacgcgtct
    46141 tgcgaaaata tagtggattg gcacgaacgt ttccccgaat tggaagcgca actgggtgcg
    46201 ccgattgagg attacgcgcc gccgaaacat tggacgcgca agcagctcag aagtatgggg
    46261 cgtgtgtcgt atttgtgcgt cgatgcggcg gagcaggctt tgacggatgc cggtttgctc
    46321 ggagacgaaa gcattaccga cggacgcatg ggcgttgcct gcggctcttc cagcggcagc
    46381 accaaagaca tcggcgatgt aggcgaattg ttgctgaccg gcatgtcgcg caacttcagt
    46441 gccaacacct atgtgcgtat gatgccgcac accgccgccg ccaatatcgg catctttttc
    46501 gggctgaaag gacgcatcat cccgacatcg agcgcgtgtt cgtccggcag ccaaggcata
    46561 ggttatgcct acgaagccat caaatacggt ctgaccgata tgatgctggc gggcggaggc
    46621 gaagaatttt tcccgtccga agtgtatgtt ttcgactcgc tttatgccgc cagccgccgc
    46681 aacggcgaac cggaaaaaac cccgcgccca tacgacgcga accgcgacgg gctggtcatc
    46741 ggcgaaggcg cggggatttt cgtgctggaa gaattggaac acgccaaacg gcgcggtgcg
    46801 ataatttacg ccgaactcgt cggctacgga gccaacagcg atgcctacca tatttccacg
    46861 ccccgccccg acgcgcaagg cgcaatcctt gcctttcaga cggcattgca acacgcaggt
    46921 cttgcgcccg aagacatcgg ctggattaat ctgcacggca ccgggacgca ccacaacgac
    46981 agtatggaaa gccgtgccgt tgcagcggtt ttcggcaaca atacgccctg cacgtccacc
    47041 aagccgcaaa ccggacacac gctgggtgcg gcgggcgcaa tcgaagccgc gttcgcgtgg
    47101 ggcattgccg accggcaaag caatcccgaa ggaaaacttc cgccccagct ttgggacggg
    47161 cagaacgacc ccgacctgcc cgccatcaac ctgaccggca gcggcagccg ctgggaaacc
    47221 gaaaaacgca ttgccgccag ctcgtcgttt gccttcgggg gaagcaactg cgtcttaatc
    47281 atcggatgaa ataagtttgt caatcccacc gctatgctat acaatacgcg cctactcttg
    47341 acgggtctgt agctcagggg ttagagcagg ggactcataa tcccttggtc gtgggttcga
    47401 accccaccgg acccaccaat tcccaagccc ggacgtatgt ttgggctttt ttgccgccct
    47461 gtgaaaccaa aatgctttga gaaaccttga taatgaaaaa agtcagcgta ttgattgttg
    47521 ccaaaaacga agcaaaccac attcaggaat gtattgaaag ttgccgtttc gataaagaag
    47581 tcatcgttat cgacgactac agcaccgaca atactgccga aattgccgag ggtttgggcg
    47641 caaaagtctt cagacggcat ttgaatgggg atttcggagc gcaaaaaaca tttgccatcg
    47701 aacaggcagg cggagaatgg gttttcctga ttgatgcaga cgaacgctgc acgccggaac
    47761 tatctgatga aatctcaaaa attgtccaaa ccggcgatta tgccgcctat tttgtcgaac
    47821 gccgcaacct tttccccaac catcccgcca cacacggcgc gatgcgtccc gacagcgtat
    47881 gccgtctgat gccgaaaaaa gacagttcgg tgcaaggcaa agtacacgaa accgtacaaa
    47941 ccccctaccc caaacgccgt ctgaagcatt ttatgtacca ttacacgtac gacaactggg
    48001 aacaatattt caacaagttc aacaaatata cttccatttc agccgaaaaa taccgagagc
    48061 agggaaagcc cgtgcgtttc gttagggaca ttatcctccg cccgatttgg gggtttttca
    48121 aaatttatat cctgaacaaa gggtttctcg atggaaaaat gggttggatt atgtccgtca
    48181 accacagcta ttacacgatg attaaatatg tcaaactata ttatctgtac aaatctggcg
    48241 gaaaatttta aatggaaaaa gaattcagga tattaaatat cgtatcggcc aagatttggg
    48301 gtgggggcga acaatatgtc tatgatgttt caaaagcatt ggggcttcgg ggctgcacaa
    48361 tgtttaccgc cgtcaataaa aataatgagt tgatgcacag acgcttttcc gaagtttctt
    48421 ccgttttcac gacacgcctt cacacgctca acgggctgtt ttcgctctgc gcgcttaccc
    48481 gctttatccg gaaaaaccgc atttcccacc tgatgataca caccggcaaa attgccgcct
    48541 tatccatact tttgaaaaaa ctgaccgggg tgcgcctgat atttgtcaaa cataatgtcg
    48601 tcgccaacaa aaccgatttt taccaccgcc tgatacagaa aaacacagac cgctttattt
    48661 gcgtttcccg tctggtttac gatgtgcaaa ccgccgacaa tccctttaaa gcaaaatacc
    48721 ggattgttca taacggtatc gataccggcc gtttccctcc ctctcaagaa aaacccgaca
    48781 gccgtttttt taccgtcgcc tacgccggca ggatcagtcc agaaaaagga ttggaaaacc
    48841 tgattgaagc ctgtgtgata ctgcatcgga aatatcctca aatcaggctc aaattggcag
    48901 gggacggaca tccggattat atgtgccgcc tgaagcggga cgtatctgct tcaggagcag
    48961 aaccatttgt ttcttttgaa gggtttaccg aaaaacttgc ttcgttttac cgccaaagcg
    49021 atgtcgtggt tttgcccagc ctcgtcccgg aggcattcgg tttgtcatta tgcgaggcga
    49081 tgtactgccg aacggcggtg atttccaata ctttgggcgc gcaaaaggaa attatcgaac
    49141 atcatcaatc ggggattcta ctggacaggc ttacgcctga atctttggcg gacgaaatcg
    49201 aacgcctcgt cttaaacccc gaagcaaaaa acgcactggc aacggcagga catcaatgcg
    49261 tcgccaaccg ttttaccatc aaccataccg ccgacaaatt attggatgca atataaactg
    49321 ctttcagacg gcctatgccg tctgaaagcc tttgatgcaa caaaccgcca aattatattc
    49381 gttcattgga aagaaacacc ccgaattcat ccttcaaaat aagaaaatcc caatatcccc
    49441 cgatattacg cagcctattg gcaaagtttt gcagcgtctt ccccggcttg tgccgccgcg
    49501 tcaagtgctt tgttacaatg cactaccttc acatttctta ataaatttta tgagtaacca
    49561 tacttcttgg tcgtccaaaa tcggtttcgt acttgctgca gcaggttcgg ccatcggttt
    49621 gggcgcgatt tggaaatttc cttatacggc aggcaccaac ggcggcgcgg tgtttttcct
    49681 gctgtttttg atatttacta tcttggtcgc cctacccgtt cagcttgccg aattttatat
    49741 cgggcgcacg ggcggtaaaa atgccgtcga ttccttcagg gttctgcgtc cgggcacgca
    49801 atggctttgg gtcgggcgta tgggcgttgc cgcctgcttt attttgctgt cgttttacag
    49861 cgtggtcggc ggatgggtat taaattatgt cgtccacagt tttacggggg cggttcatac
    49921 cggcgcggac tttgaagcct tgttcggcgc gacgatttcc aatccggcag gttcgctgtc
    49981 ctatcaggct ctgtttatgc tgattacggt ttgggtggtc aaaggtggga tttcagacgg
    50041 cattgaaaag gcaaaccgtt atctgatgcc ggggctgttt atcctcttta ttgcgctggc
    50101 aatccgttcg ctgacgctgc cgggtgcaat ggagggcgtg tcattcctgc tcaaaccgaa
    50161 ttggtcgtac tttaaagccg atacgatgat tacggctttg ggacaggcgt tttttgccct
    50221 gagcatcggt gtttccgcca tgattaccta cgcttcatat ttgggaaaag atcaggatat
    50281 gttccgctcg gggcatacga ttatgtggat gaacctcttg gtttcgctgc ttgccggcct
    50341 ggtgattttt ccggcggtgt tcgccttcgg ttttgaaccg agccaggggc cgggattgat
    50401 ttttatcgta ttgcccgcag tgtttatgaa gatgccgttc ggtacggttt tgtttgcggt
    50461 atttatgctg ctggtcgttt tcgccacgct gacttccgca ttttcgatgt tggaaacggt
    50521 catcgccgca accatccgcc aagacgagcg caaacgcaaa aaacacactt ggcttatcgg
    50581 cacggccatt ttcattatcg gcatcccatc cgcgctgtct ttcggcgtat ggggtgagtt
    50641 taaggttttc ggcaaaacca tttttgattt gtgggactat gttatttccg ccgtcattat
    50701 gccgattggt gctttgagtg tttccatctt taccgcctgg attcaggaca agcagtctgt
    50761 gttaaaagat gccggcgcgg gcagcaccgt accacgggca gtgctgctgc tgtggctgaa
    50821 taccttgcgc taccttgccc cgattgccat tattattgtt ttcatcaatt ctttggacat
    50881 cctttaaaag ccatccaaac agcaaaaatg ccgtctgaaa gcctttcaga cggcattttt
    50941 gcttcaggtt cagcctattt cgttcaaagt atagtggatt aacaaaaatc aggacaaggc
    51001 gacgaagctg cagaccgtac agatagtacg gaaccgattc acttggtgct tcagcacctt
    51061 agagaatcgt tctctttgag ctaaggcgag gcaacaccgt actggttttt gttaatccac
    51121 tatagccttg cgcgatgccg ttcaaggaca aacccatacc cttttcggca aaacggattt
    51181 cacggtcgtc aaacgagact ttgccgaagc cgacccgttt cagggcttcc ttcacgctgt
    51241 tttgaggcgg cggcgtttcc gcatcgggac gggcggcaaa ataatcggca tacagtttcc
    51301 acaacgcctg cactgtcgga tcgaacgcgc cgtccgtcag cgcgtgaata tcgcggcaca
    51361 ggctcaacag ttccaaaaaa tccgccgacg gcgaagtcag ataaccgtcc ctgttcaggc
    51421 ggctgatcag gctgtcttca cggtaaaggc tgaacaattt tttccaaacg cgccacttcc
    51481 gccaaaacct tgttgaccaa atccgccgca cgcctgtcgt ccacaccgaa cagacggagc
    51541 tccgcaccgg aacccagtgc gacacctttc cagaaaaaca cattttcact gcgtttttca
    51601 tccccgttgc gtttttcatc atcggcggca aaaggattcg gcaaaaaaga aaccgccgcg
    51661 cccgccgccg caacggcggc agccgtcaaa aaacgcctgc gcccgaaatg cctgcccata
    51721 ccgcctccta aaccgacact gccgccttga tgtgcggatg cggatcgtag ccttccaact
    51781 cgaaatcttc aaacttgaag gaaaacaaat ctttgacttc aggattgatt ttcatcactg
    51841 gcaaggcgcg cggttcgcgt tccaactgca atgcggcctg ctcgaaatgg ttgcggtaca
    51901 aatgcgcgtc gccaaacgta tggacaaact cgcccgcctc cagcccgcac acttgcgcca
    51961 tcatcatcgt caacagcgcg tagctggcaa tattaaacgg cacaccaagg aaaatatctg
    52021 cactacgctg gtaaagctgg caggacagtt tgccgtcggc aacgtaaaac tgaaacagcg
    52081 tgtggcaggg cggcaaggcc atttcatcga ccaaagccgg attccacgcc gatacaatca
    52141 ggcggcgcga gtcgggattc ttcttgattt gttccagcac attggcgatt tggtcgatat
    52201 gcctgccgtc gggcgcgggc cagttacgcc actggtagcc gtaaaccggg cctaagtcgc
    52261 cgttttcgtc cgcccactcg tcccaaatgg aaacattgtt gtcctttagg tatttgatat
    52321 tggtatcgcc tttgagaaac caaagcagct cgtggataat cgaacgcaga tgcagctttt
    52381 tggtcgtcag cagcggaaaa cctttgccca agtcaaaacg catctgataa ccgaatacgg
    52441 agcgcgtacc cgtaccggtg cggtctgatt tgtccgtacc gttgtcgagg acgtggcgca
    52501 tcaagtccaa ataggctttc atagcagtct ttcatcaaat taaacggcgc atattgtaac
    52561 atttccggat aatgcccaaa acacggatac aggcaggcag gattgttggc aatttcagtc
    52621 cttttccaca gtaaaacccg gtgggaaaac aaaattacct tgattggaat caaaaaatct
    52681 agtttaatta cttagaataa aatttcaata atatttttat ttacgaaatt aatttatgat
    52741 tattgattaa ttatcggcaa caaacatcaa acatcgaaaa catggaaaaa ataatgtcaa
    52801 caatttttgc caaatcgggc ttggcatcag aaaaaaatag gtttatattc ccacctacaa
    52861 atttgttttc ccattagtac actatcaacc aaaaggagta tccgaatgac tgacctgaac
    52921 accctgtttg ccaacctcaa acaacgcaat cccaatcagg agccgttcca tcaggcggtt
    52981 gaagaagtct tcatgagtct cgatccgttt ttggcaaaaa atccgaaata cacccagcaa
    53041 agcctgctgg aacgcatcgt cgaacccgaa cgcgtcgtga tgttccgcgt aacctggcag
    53101 gacgataaag ggcaagtcca agtcaaccgg ggctaccgcg tgcaaatgag ttccgccatc
    53161 ggtccttaca aaggcggcct gcgcttccat ccgaccgtcg atttgggcgt attgaaattc
    53221 ctcgcttttg aacaagtgtt caaaaacgcc ttgaccaccc tgcctatggg cggcggcaaa
    53281 ggcggttccg acttcgaccc caaaggcaaa tccgatgccg aagtaatgcg cttctgccaa
    53341 gcctttatga ccgaactcta ccgccacatc ggcgcggaca ccgatgttcc ggccggcgac
    53401 atcggcgtag gcgggcgcga aatcggctac ctgttcggac aatacaaaaa aatccgcaac
    53461 gagttttctt ccgtcctaac cggcaaaggt ttggaatggg gcggcagcct catccgtccc
    53521 gaagcgaccg gctacggctg cgtctatttc gcccaagcga tgctgcaaac ccgcaacgat
    53581 agttttgaag gcaaacgcgt cctgatttcc ggttccggca acgtggcgca atacgccgcc
    53641 gaaaaagcca tccaactggg tgcgaaagta ctgaccgttt ccgactccaa cggcttcgtc
    53701 ctcttccccg acagcggtat gaccgaagcg caactcgccg ccttgatcga attgaaagaa
    53761 gtccgccgcg aacgcgttgc cacctacgcc aaagagcaag gtctgcaata ctttgaaaaa
    53821 caaaaaccgt ggggcgtcgc cgccgaaatc gccctgccct gcgcgaccca gaacgaattg
    53881 gacgaagaag ccgccaaaac cctgttggca aacggctgct acgtcgttgc cgaaggtgcg
    53941 aatatgccgt cgactttggg cgcggtcgag caatttatca aagccggcat cctctacgcc
    54001 ccgggaaaag cctccaatgc cggcggcgta gcaacttcag gtttggaaat gagccaaaac
    54061 gccatccgcc tgtcttggac tcgtgaagaa gtcgaccaac gcctgttcgg catcatgcaa
    54121 agcatccacg aatcctgtct gaaatacggc aaagtcggcg acacagtaaa ctacgtcaat
    54181 ggtgcgaaca ttgccggttt cgtcaaagtt gccgatgcga tgctggcgca aggcttctaa
    54241 gcaaacgccg ccgctccgca aacaaaatgc cgtccgaacc gcaaatgctg ttcagacggc
    54301 atttcattat ccgcccgttc gaatcgggtg atactatttg tatccagaat tgttggatag
    54361 tttgggagtg gagaggaaga aatgattatg gaccaatact atatggagtt aaagaataag
    54421 ttatctaaca gaccaatcct actggataat actaatgatt ttctttttgt tttagtaaat
    54481 acagtgaaag ctatgataga aaatactgat aaaagtcagc tttcagagtt agataaaatt
    54541 ttagacggtg tcacaagtca agaattgaaa cttgcctatg atttttgtca agggaaattc
    54601 ggtcaagcag gtttttctta tcgtaggcac ccgaattatt tttatttgtc cagtttaatt
    54661 gcgacttttc cagaatttga gttgtctaaa gcagaccgag attacctcaa aggaatcata
    54721 aattttgata actacctttt atatgagtta gattagtctt taatcacctc aaaaccctat
    54781 ttattcaaaa ggagaaaaaa tgattgagct gaaattagta gatgagagca gttttcaggc
    54841 agtgctggat ttgaaaatat ctgaagctga tgaacgagca cgttttgtgg ctccaaatgt
    54901 acgctcttta gctgacgcat ggctctaccg ggaaaatgaa gatgtgtttc cgagggcaat
    54961 ctattgggat aagcaagtgg ttggctttct cctgctggaa atagacaagg atgaagcaga
    55021 atactttatc tggcggataa tgattggtca gcagtgaatt aacaaaaatc aggacaaggc
    55081 ggcgagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta
    55141 gagaatcgtt ctctttgagc taaggcgagg caacgacgta ctggtttaaa tttagttcac
    55201 tataccgata catccgaata tgccagaccg tctaaacggt attcatacct cccaatcctg
    55261 cacgcgcttc aaatcgtttt gcgccaaagt atccgcgtgg cggttacggc tctgatattc
    55321 cctgtctttc atgatgctgc ttgccacata attcaaatgt gcctttgccg cctccgaagc
    55381 ctcgcccggc cgacggttcg atattgcctc atacaataca cggtgctgcg ccatcagctt
    55441 cggacgcgga tcttcttcct gattcagata aataaggctg ctgcgcgtct gccggtacag
    55501 cattttcaac aaaccgcccg acaaatggct gaacaacaaa ttgtgcgccg catcggcaat
    55561 cgtctgatga aagctgacat cagcttcgct ctgatgttcc aaattgccgc tttcgcacgc
    55621 ctcctcaaac ttttcaagcc aaaacccaat ccgcttcaaa tcggcatccg tgcggcgttc
    55681 tgccgccaat gccgccatac agccctcgat gtggcaactg aaatcaaaaa catcctgttc
    55741 ccaattggaa tgcttgccca aaagctcctg ccaactttgc aaaaaatcct gctgcggctt
    55801 gaccgaaaca taataaccgt ctccctgcct cgcttccaaa acctgacggg cgaccaaaac
    55861 attcaatgcc gacctgaccg acgggcgcga aacgccgaac tcttccgcca aaacgcgttc
    55921 gggcggaatc ttgccccctt ccgcgtaaac cccttccgca atgcgctcct ccaataccga
    55981 caatacctga tcgctgattt tctgaggcct taccagtttc atcactcctc ctttataaag
    56041 attccctgca gaacccttcc gaaatatagt ggattaacaa aaatcaggac aaggtgacga
    56101 agccgcagac agtacaaata gtacagaacc gattcacttg gtgcttcagc accttagaga
    56161 atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttca tccactatac
    56221 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
    56281 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
    56341 atttttgttc atgcaaatat ttgttttgac aggatttaaa caaaagctcc gattcaaatc
    56401 tgccgaaccg cccaaaaata tattgaccta aatattaaag tttcgtaaag taatgcaacg
    56461 ttgctttaat tggtttgacc actattgccg acgattagaa aaatattttc ggagatgttc
    56521 aattatggaa acttgggttc aaaactacac ggcaatcggc ggcagcctgt atctgactgc
    56581 cgccgccgca ctcttaccca tcgtcttttt ctttgccgcg ctgaccgtcc tgaagctgaa
    56641 aggctatcag gcggggcttt atacgctgct gattgcgctt gccgttgccg tattcggctt
    56701 cgggatgccg acgggtatgg cggtttcttc cctgccgccg cagccgcatt gacccaacgc
    56761 ccctacgcca cactgtattt gaccgcgcat tacaaaatcg gcaaatccac ccgcatcggt
    56821 ttggactttg aaaacgtgtt caacaaacgc taccgcccta tgcccgacat tcacgtttac
    56881 ggcacgccgc gcagcctgac cgcaaccgt
//
LOCUS       DAWFKN010000004        50211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFKN010000004 DAWFKN010000000
VERSION     DAWFKN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 50211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.004"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..68)
                     /locus_tag="WGR46_000227"
     CDS             complement(<1..68)
                     /locus_tag="WGR46_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0855249.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGR46_000228"
     CDS             complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGR46_000228"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0855250.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1109..2195)
                     /locus_tag="WGR46_000229"
                     /pseudo
     CDS             complement(1109..2195)
                     /locus_tag="WGR46_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(2468..4354)
                     /gene="uvrC"
                     /locus_tag="WGR46_000230"
     CDS             complement(2468..4354)
                     /gene="uvrC"
                     /locus_tag="WGR46_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0855251.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(4409..6586)
                     /locus_tag="WGR46_000231"
     CDS             complement(4409..6586)
                     /locus_tag="WGR46_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0855252.1"
                     /translation="MQQKVRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            6725..7675
                     /gene="trxB"
                     /locus_tag="WGR46_000232"
     CDS             6725..7675
                     /gene="trxB"
                     /locus_tag="WGR46_000232"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0855253.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGTELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            7831..8199
                     /gene="rpsF"
                     /locus_tag="WGR46_000233"
     CDS             7831..8199
                     /gene="rpsF"
                     /locus_tag="WGR46_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0855254.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8200..8502
                     /gene="priB"
                     /locus_tag="WGR46_000234"
     CDS             8200..8502
                     /gene="priB"
                     /locus_tag="WGR46_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0855255.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            8509..8739
                     /locus_tag="WGR46_000235"
     CDS             8509..8739
                     /locus_tag="WGR46_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0855256.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8756..9208
                     /locus_tag="WGR46_000236"
     CDS             8756..9208
                     /locus_tag="WGR46_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0855257.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            9222..9329
                     /locus_tag="WGR46_000237"
     CDS             9222..9329
                     /locus_tag="WGR46_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0855258.1"
                     /translation="MPSETLPIVGMWFQTVFVLFFVYSAKIGMVVFIVD"
     gene            complement(9996..10802)
                     /locus_tag="WGR46_000238"
     CDS             complement(9996..10802)
                     /locus_tag="WGR46_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0855259.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(10803..11549)
                     /gene="pssA"
                     /locus_tag="WGR46_000239"
     CDS             complement(10803..11549)
                     /gene="pssA"
                     /locus_tag="WGR46_000239"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0855260.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12132..12615
                     /locus_tag="WGR46_000240"
                     /pseudo
     CDS             12132..12615
                     /locus_tag="WGR46_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(12667..13878)
                     /locus_tag="WGR46_000241"
     CDS             complement(12667..13878)
                     /locus_tag="WGR46_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0855261.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14101..16539
                     /locus_tag="WGR46_000242"
     CDS             14101..16539
                     /locus_tag="WGR46_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0855262.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            16747..18057
                     /locus_tag="WGR46_000243"
     CDS             16747..18057
                     /locus_tag="WGR46_000243"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0855263.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18153..18767
                     /gene="clpP"
                     /locus_tag="WGR46_000244"
     CDS             18153..18767
                     /gene="clpP"
                     /locus_tag="WGR46_000244"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0855264.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(18820..20085)
                     /gene="ispG"
                     /locus_tag="WGR46_000245"
                     /gene_synonym="gcpE"
     CDS             complement(18820..20085)
                     /gene="ispG"
                     /locus_tag="WGR46_000245"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0855265.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20101..20862)
                     /gene="pilW"
                     /locus_tag="WGR46_000246"
     CDS             complement(20101..20862)
                     /gene="pilW"
                     /locus_tag="WGR46_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0855266.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(20865..21959)
                     /gene="rlmN"
                     /locus_tag="WGR46_000247"
     CDS             complement(20865..21959)
                     /gene="rlmN"
                     /locus_tag="WGR46_000247"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0855267.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22103..22528)
                     /gene="ndk"
                     /locus_tag="WGR46_000248"
     CDS             complement(22103..22528)
                     /gene="ndk"
                     /locus_tag="WGR46_000248"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0855268.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(22587..23738)
                     /locus_tag="WGR46_000249"
     CDS             complement(22587..23738)
                     /locus_tag="WGR46_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0855269.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(23922..24025)
                     /locus_tag="WGR46_000250"
                     /pseudo
     CDS             complement(23922..24025)
                     /locus_tag="WGR46_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24132..24959)
                     /gene="fghA"
                     /locus_tag="WGR46_000251"
     CDS             complement(24132..24959)
                     /gene="fghA"
                     /locus_tag="WGR46_000251"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0855270.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(24968..26077)
                     /locus_tag="WGR46_000252"
     CDS             complement(24968..26077)
                     /locus_tag="WGR46_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0855271.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGVGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            26225..26632
                     /locus_tag="WGR46_000253"
     CDS             26225..26632
                     /locus_tag="WGR46_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0855272.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(26983..27297)
                     /locus_tag="WGR46_000254"
     CDS             complement(26983..27297)
                     /locus_tag="WGR46_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0855273.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(27308..28993)
                     /gene="rpsA"
                     /locus_tag="WGR46_000255"
     CDS             complement(27308..28993)
                     /gene="rpsA"
                     /locus_tag="WGR46_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0855274.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(29149..29805)
                     /locus_tag="WGR46_000256"
     CDS             complement(29149..29805)
                     /locus_tag="WGR46_000256"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0855275.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRDSLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(30049..30858)
                     /locus_tag="WGR46_000257"
     CDS             complement(30049..30858)
                     /locus_tag="WGR46_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0855276.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIYVFGNIPSILSYGPWKDVSVFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFVSNLF"
     gene            complement(30918..31361)
                     /locus_tag="WGR46_000258"
     CDS             complement(30918..31361)
                     /locus_tag="WGR46_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0855277.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            complement(31890..32582)
                     /locus_tag="WGR46_000259"
     CDS             complement(31890..32582)
                     /locus_tag="WGR46_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0855278.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPAGETLCETLKNGVLLHD
                     ENETVYAADAVLESPSALLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            complement(32650..34626)
                     /locus_tag="WGR46_000260"
     CDS             complement(32650..34626)
                     /locus_tag="WGR46_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0855279.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            34804..35469
                     /locus_tag="WGR46_000261"
     CDS             34804..35469
                     /locus_tag="WGR46_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0855280.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            35521..36348
                     /gene="mutM"
                     /locus_tag="WGR46_000262"
     CDS             35521..36348
                     /gene="mutM"
                     /locus_tag="WGR46_000262"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0855281.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            36436..37203
                     /locus_tag="WGR46_000263"
     CDS             36436..37203
                     /locus_tag="WGR46_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0855282.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(37515..37826)
                     /locus_tag="WGR46_000264"
     CDS             complement(37515..37826)
                     /locus_tag="WGR46_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0855283.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
     gene            38355..40634
                     /gene="nrdA"
                     /locus_tag="WGR46_000265"
     CDS             38355..40634
                     /gene="nrdA"
                     /locus_tag="WGR46_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0855284.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            40692..41705
                     /gene="dcm"
                     /locus_tag="WGR46_000266"
     CDS             40692..41705
                     /gene="dcm"
                     /locus_tag="WGR46_000266"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0855285.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            41698..42885
                     /locus_tag="WGR46_000267"
     CDS             41698..42885
                     /locus_tag="WGR46_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0855286.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            42946..44079
                     /gene="nrdB"
                     /locus_tag="WGR46_000268"
     CDS             42946..44079
                     /gene="nrdB"
                     /locus_tag="WGR46_000268"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0855287.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            44277..44567
                     /locus_tag="WGR46_000269"
     CDS             44277..44567
                     /locus_tag="WGR46_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0855288.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(44608..44886)
                     /gene="ftsB"
                     /locus_tag="WGR46_000270"
     CDS             complement(44608..44886)
                     /gene="ftsB"
                     /locus_tag="WGR46_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0855289.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(44900..46186)
                     /gene="eno"
                     /locus_tag="WGR46_000271"
     CDS             complement(44900..46186)
                     /gene="eno"
                     /locus_tag="WGR46_000271"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0855290.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            complement(46233..46673)
                     /locus_tag="WGR46_000272"
     CDS             complement(46233..46673)
                     /locus_tag="WGR46_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0855291.1"
                     /translation="MMQTFRKISRYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            complement(46688..47530)
                     /gene="kdsA"
                     /locus_tag="WGR46_000273"
     CDS             complement(46688..47530)
                     /gene="kdsA"
                     /locus_tag="WGR46_000273"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0855292.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFCVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(47552..47935)
                     /locus_tag="WGR46_000274"
     CDS             complement(47552..47935)
                     /locus_tag="WGR46_000274"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0855293.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(48084..>50211)
                     /gene="mfd"
                     /locus_tag="WGR46_000275"
     CDS             complement(48084..>50211)
                     /gene="mfd"
                     /locus_tag="WGR46_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0855294.1"
                     /translation="AIAVITESDLYQYVARSRKYHRKKHAAVSDGLLRDLAEINIGDP
                     VVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENI
                     ALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADGFG
                     YEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLA
                     PTTLLVEQHAQNFADRFADFPVKVAGLSRFNNSKATKAALEGMADGTVDIIIGTHKLV
                     QDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRD
                     FSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLE
                     TLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRA
                     DKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQ
                     DLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKL
                     HSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESH
                     HLRLAAKELGIDAIDAAGESVTVTFGKNHNVDPTEIILLIQNDKKYRLAGADKLRFTA
                     EMENIEVRINTVKNVLKTLQNRCLPK"
BASE COUNT        11944 a        11838 c        13585 g        12844 t
ORIGIN      
        1 ggagcagttc tttctgtact tttttcttta atttgcagtg cgttatcttc atatttcgag
       61 ggtaacatat ctgctaatct agtacagccc caactttaaa accgatgtgc aggttaaaac
      121 ctacaaggat gcgtgcggta tacacgcgtt tttgccgttc aaaccattgc tttgaacttt
      181 gtatcgaaac atcgttcgct gctttatctt gaaaaaatgc cgtctgtaag caaacatgct
      241 tcagacggca tttttttatc cggcggtttg ttatccgatc cgtttgaata ccaagtccca
      301 aacgccgtgt ccgaggcgtt tgccgcgcgc ttcgaatttg gtttcggggc ggtagtccgg
      361 ggtgggggcg tagtctgccg ccgtattttg caggctgtcg aagctgctga ggacttcaag
      421 catctgctgt gcatattctt cccagtctgt cgccaggtgg atatagccgc cggttttgag
      481 tttgggcagc agtttggcga tgaagggggc ttgtatcaga cggcgtttgt ggtggcgttt
      541 tttgtgccac gggtcgggga agaatatgtg gatgccgtcg agcgagccgt cttgcagcat
      601 attttcgaca acttctacgg cgtcgtgccg catcacgcgg atgttttcca aatggttttc
      661 ttctatgagt ttgagcagat tgcctacgcc gggaccgtgt acgtcgatgg caagaaagtc
      721 ggtttcgggc agcctgcggg cgatttctgc ggttgccgtc cccataccga agcctatttc
      781 gaggattttg ggtcggctgc ttccgaaacg ggcatcaaga tcggccggtg ttgcttggta
      841 gtcgatgccg aagctgcccc ataaggtatc gatggcgcgt tgctgcgcgg cggtcatgtg
      901 gctttggcgg aggacgaaac tgcggatgct gcgggggtgt tcggcttgcc agttgttttg
      961 tgtttggttt tcggcggtgt ctgtcataat gctttcgtgc gcttgtgcaa tcaggatgcc
     1021 gtctgaatgt gaaaggcctg ttcggacggc gggaaaggaa aggggcgatt atacctgttt
     1081 cgtttcggca gggtaggtgt aacgctgttc agacggcatt ttgccattgt tgcagcagtt
     1141 cttgcaggac gcttttttgc ccgtatccgt tgtttatggc ttcttgcagg tagcggcagg
     1201 ctttttcttt gtccggctcg acgccgtgtc cgttgtaatg gatgcaggcg aggttataaa
     1261 gtgccatgct gtctttttgc gcggcggctt tttcaaacca tttgcgtgct tcattgtagt
     1321 cggcggtcat accctgtccg aagtaataca tgctgcccag attggtttgg gctttgctgt
     1381 ggccgagttc tgcggcttct gtgtagagtt tgagtgcggc tgcatattcg ggcgcgcatt
     1441 gtaatccgta atgcagggct tgggcttttt gataaagccg ttctgccctt tgccgcctgt
     1501 tgaaaccagt gaacggcttc atcaggagcc gtaggtagga tgagctcggc aagcggggtg
     1561 taggcgggaa tgtatccttg ttgtgcggcg gcgcgcaaat gattgacggc ctgtgccgtg
     1621 tcgactttcg tcccttgtcc gaaacggtat tgcagcccga gctgccagtg tgccgccgcc
     1681 aagccttgtg ccgccgcttt gcggtagagc aggtgcgccg tttcaaaatc cggcggcgtg
     1741 ccgtaccggc agtattgggc aagcaggtat tgcccgtggg ggttgttgtt ttttgcgctt
     1801 gtttttgccc atttcagggc ttcttcaaaa tgtcgggacg catacagaag gtacatcagg
     1861 cggacttgtg cttcaggcag tccttcctct gccaacggtc tgtaaagcgg tatcagggtt
     1921 tccgggggcg cgccttccaa ttcttggata tatgccagat tgtaacgcgc atagggatgc
     1981 ccctgttcgg cggcgatgcg gagcatttcc ataccttgtt cgcggtcttg gtaggggctg
     2041 tccgcgtcca tcaggtgttt tgccaactcg aatgcggcgg cgggatgacc tgccgagaac
     2101 gcttctgtca gcagtcggcg gacttcggta aagttatatt gtttttgaga caggcattct
     2161 aaggcctgcc gatagggtaa gtcgggattg ttcatgtcag atcgttttcc gttttgtcga
     2221 atcatcgtta ttgtgttatg aagttaggtt tatagtggat taacaaaaat caggacaagg
     2281 cgacgaagcc gcagacagta caaatagtac gaaaccgatt cacttggtgc ttgagcacct
     2341 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggttta aatttaatcc
     2401 actatatatc ggtataccga cctgctgtat catatttttt acagacggtt tttgcttggg
     2461 cagcatacta atgcagatgc tcgtaaatgg tttccgccaa tgccttgctg atgccttcca
     2521 ctttttccaa gtcctcgcgg ctggcggcaa tcacgccgcg cagaccgccg aagcgggtga
     2581 gcagggcttg gcggcgtttg ctgcctacgc cggggatgtc gcttaaggag gaggtaacgc
     2641 gggctttgtc gcgttttttg cggtggccgg taatggcgaa acggtgcgat tcatcgcgta
     2701 cggtttgcaa tagatgcaag gccgggctgt tgggcggcag gcggaagact tcgccggtaa
     2761 aaggcagtat gagttcttcc ataccggctt tgcgttcggg gcctttggcg ataccgacca
     2821 aaggaatgtg cagcccgagt tcttcccata ccgatacggc gatgccgatt tgccctttgc
     2881 cgccgtcaat taacacggca tccggccatt tgacggtctc gccgttggct tcggcttcct
     2941 gcattttgcc gtaacggcgc gtcaacactt cgcgcatggc ggcgtagtcg tcgccgggtt
     3001 tggcggtcgt gatgttgtag cggcggtatt gcgagggctg gatgttttgc tcatcgtaca
     3061 cgacgcagga ggcgatggtg gcttcgcctt gggtgtggct gatgtcgaag cattcgaggc
     3121 ggttgaggcc gtctgaatcc ataccgagga ttttggcgag ttcgtcgatg cggtgttgct
     3181 ggttgctttg ttgcaggcgg cgttgtgcaa tcgccatttg tgcgttttgt tccgccattt
     3241 tcaaccaaac tttgcgttcg cctatggttt tggttacgaa ctgcatttgt ttgccgtgtt
     3301 cgccttccaa agcctctttc agcgcatcgg ggacggggaa gttgttgatg ataatgtcgg
     3361 gtttgctttt gcccagatag tgttgggcga cgaaggcttc ggcatagtct tgcccgtttg
     3421 gctcgggatc gtttttggtg tcggggaaga aacttttgtc tccgacgtgc cgcccaccgc
     3481 gtatgctgac ccagtgtacg cagacgatgc cgtctgaaac cgccagcgcg agcaaatcga
     3541 tatcgttggg gttgttcggg tttttgctgt cgatgaactg gttgctctgc ataatgccga
     3601 gtgcttggat ttggtcgcgg tagcgggcgg cttcttcaaa ttgcagattc gcggcggcag
     3661 tttgcatttt gtgttgcagg gtgcgggtca gttcgtcggt tttgccgttg agaaaagtgg
     3721 cggcttgacg cacgctgtcg cggtagtctt cttcgctgat gtgtccgacg cagggcgcgg
     3781 tgcagcgttt gatttgatac agcaggcaag ggcggtcacg atgctcgaat acgctgtctt
     3841 cgcaggtacg cagcataaag actttttgca acacttgaat gctgtcgcgc acggcgttgc
     3901 tgttgggata cgggccgaag tattggtttg gctttttcag cgtgccgcgg taatacgcca
     3961 tctgcggata ttgatggccg ctgagcatca aataaggata gcttttgtcg tcgcggaaaa
     4021 ggatattgta tttcggcgac aaggctttga tgaggttgtt ttcgagaatc agggcttcgg
     4081 cttcggaacg cgtgatggtg gtttcgatat gatgaacctg ttttaccatc aatgcgatgc
     4141 gcggcgaatg gtcgtttttt tggaaatagc cggacacgcg ccgcttgagg ttgaccgctt
     4201 tgccgacgta taagactttg ccgccttcgt caaaaaaacg gtacacgccc ggcagcttgg
     4261 gcaggttttt gaggaagagc gggatatcga attgttccgg agaacggttt tctgtattca
     4321 tatcaattat ttgaaaaacg gcttcagacg gcatatcggc agcggcagcc ttatccgttc
     4381 ggaaaagctg tcgggtggtt atatgcggtc aatcgatttt tacccgtttc aggcgcaagg
     4441 cgttgctcaa cacggaaacc gagcttgccg ccattgccgc gcctgcgatg acagggttta
     4501 aaaaaccgag cgcggcaagc ggaatgccca aaatattgta gaagaaggcg aaaaacaggt
     4561 tttgcttgat gtttctcaac gtcgcccgcg ataccgatag ggcatcggcg agttggttga
     4621 ccgaatgttg catcagcgtg gcggatgcgg tgtgttcggc aacgtccgcg ccgcctttca
     4681 tggcgaagct aacgttggcg gcggcgagcg caggtgcgtc gttgatgccg tctccgacca
     4741 tcgccacggt tttgccggcg gctttgagtt tctgcacttc ggcggctttg tcgcgcggac
     4801 tcatattacc gaaggcgtgt gcgatgccca gttgtttggc gacgtattcg accgtgcctt
     4861 ggttgtcgcc gctcataata tagacatcga tattgtgttt tttcagacgg cctatggctt
     4921 cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc gccgatgggt ttgttatcga
     4981 ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc taaaaacttc ggcaaggcca
     5041 gttcggcaaa ttcggctttg cctgctttca ccaaacccac gccttccact tcggcggtaa
     5101 tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg aatgtccaaa ccgcgcgctt
     5161 gggcggcgga gacgatggca cgggcgagcg gatgggcggc gttttgttcg acggcggcgg
     5221 cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg aacgcaataa acggcggcaa
     5281 cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa cacgacggca tcgacgtggg
     5341 cggcttcctc cattgctgcc gcgtctttaa accaaatacc gtgtttcacc gctttgccaa
     5401 tgccgaccat aatcgcggcg ggtgtagcca gaccgagcgc acacgggcag gcaatcacca
     5461 aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc cttaatcagc caagtaacaa
     5521 taaaagtcaa caacgcgatg cccacgacgg caggcacgaa caccgccgcc gccttgtccg
     5581 ccacgcgcgc aatcggtgct ttactgcctt gcgcttcaga gagtgcgttc atcatgtcgc
     5641 cgagcagggt ttggctgccg agctgcgcgg cgcggtacac cacgctgcct tcagtcatca
     5701 gcgcgcccgc caatactttg ccgcctgcct ttttctcttc gggattggat tcgccggtaa
     5761 gatggctttc gtccgcccag ccgctgccgc tttctatgat gccgtcggcg gcaatgcgtt
     5821 cgccgtgatt ggcgcggatt aggtcgccga tttgcacttg gtcgatgggt agctgccgcc
     5881 attcgccatc gcgttgcacg ttgacttggg ttggcgtgag tttgagcagc aagcccaagc
     5941 tgttcaggct ggattttttg gtgcggtgtt ccaaaaattt acccagtgac acaaaaccaa
     6001 tcaccattat gcctacttca aaatacacat gcgccatacc gtacgccgcg tgcgggctga
     6061 aaaacagcat atagacggaa tacaggtaaa tcgagaccgt gccgatggta acgagtacgt
     6121 ccatattcgc cagcccgcct ttaatgctcg cccacgcgct tttgtaaaat ggcaccgcca
     6181 gccaaagctg caccacactc gccaatgcaa actgcaacaa gggcgacaac atccaatcat
     6241 gccgtgtcca attcagccct tttagcatca tccctaccat accgataagg aacgggatat
     6301 tgatggccag caaaagccac aacctccagc cgatatggtg ttctgcttcg ggttgcggca
     6361 atgtatcttc cgttttttcc ttcgcgccgt aaccggtttt ctcaatgatt ttggcaatgt
     6421 cggctactga ggttttgctg tcgtcaaaca ctacctgcgc ctcttcgctg gcgaagttta
     6481 cccccgccga ttcgacaaaa tcttttttgt tcaacacttt ttcaatgcgc gaagcacatg
     6541 cctggcaggt catgccttcg atttggaaac ggactttttg ttgcataagc gtgattcctg
     6601 ctttgatatt cagacggcgg tcaaggctgc gtgatgccgt ctgaacgccg gtatggtcga
     6661 aatttctcaa acgcgccgat aattcgagta gaatccgaaa acttgacaca gcaaaggaaa
     6721 aacaatgtcc caacaccgca aactgattat tttgggttcc ggccccgccg gatacaccgc
     6781 cgccgtctat gccgcgcgtg ccaatttaaa ccccgtcatt attacaggta tcgcgcaagg
     6841 cgggcaactg atgaccacca ccgaagtgga caactggcct gccgatgccg acggcgtgca
     6901 agggacggaa ttgatggcgc ggtttctcgc ccacgccgaa cgtttcggaa cggaaatcat
     6961 ttttgaccaa atcaacgccg tcgatctgca aaaacgcccg tttacactca aaggcgatat
     7021 gggcgagtac acttgcgatg ccctgattgt cgcaacaggc gcgtccgcca aatacctcgg
     7081 tttgccgagt gaggaagcgt ttgcaggaaa aggcgtttcc gcttgtgcca cctgcgacgg
     7141 tttcttctac aaaaaccaag atgttgccgt agttggcggc ggcaatacgg cagttgaaga
     7201 ggcactctac cttgccaata tcgccaaaac cgttaccctg atccaccgcc gcagcgagtt
     7261 ccgtgccgaa aaaatcatga tcgacaaact gatgaaacgc gtggaagagg gcaaaatcat
     7321 cctcaagctg gaaagcaacc tgcaagaagt actgggcgac gatcggggcg taaacggcgc
     7381 attattaaaa aacaacgacg gttctgagca acaaattgcc gtcagcggca tttttatcgc
     7441 catcgggcac aagccgaata ccgatatttt caaaggtcag ttggaaatgg acgaagccgg
     7501 ttacctgaaa accaaaggcg gcacggcgga caatgtcggc gcaaccaata tcgaaggcgt
     7561 atgggcggcg ggcgacgtaa aagaccatac ctaccgtcag gcgattacca gcgcggcttc
     7621 cggctgtcag gcggctttgg acgcggaacg ctggctgggc agccaaaaca tttgaatgcc
     7681 gtctgaaacc gaaattttcg gcggaatgca aatttttcgc accaaatcct tgattccgtc
     7741 ggcaaacccc aatataatgc ctgctctttg aaccttgcct tctgctttcg catggcagaa
     7801 ggctgttttt accatccata aggagataac atgcgtcatt acgagatcgt gtttatcgtt
     7861 catcctgatc aaagcgagca agtgcctgct atggttgaac gttacaaaac catgattgct
     7921 gaagcaaacg gtaagattca ccgtttggaa gattggggtc gccgccaact ggcttacccg
     7981 attaacaaaa tccataaagc ccattacgtt ttgatgaaca tcgaaaccac tcccgaagtg
     8041 gttgaagagc tggaaaccgc attccgcttc aatgatgccg tattgcgcca tctaaccatc
     8101 aaaaccaaac acgccgttac cgaagcatcc cctatgttgg gtggtgaaaa ggctaagaac
     8161 ctgttgagcg gcgcgtctga agaagcggtc gcccaataat tgggattcaa taatcttgtt
     8221 tcgcttgccg tgttaattga aaaggttttc cctattcgat atacgcctgc cggaatccct
     8281 gttttagata ttattttaaa gcacgaatcg tggcaggagg aaaacgggca gcaatgcctt
     8341 gtccaattgg aaattccggc acggatttta ggcaggcagg cggaagagtg gcagtatcgg
     8401 caaggtgtat atgttcacgt cgaaggtttt ttagctcaaa aaagcagacg ttcccttatg
     8461 ccgatgctca ggatacaaaa tattcaagaa tataaaggtt aaacgacaat ggctcgtcaa
     8521 tcattcaaac gtagaaaatt ctgccgtttc acggctgaaa aaatccaaga agtcgattac
     8581 aaacaagttg atttgctgaa agactttatc tctgaaaacg gtaaaatcat tcctgcacgc
     8641 atcacaggaa cgaaggcatt ctaccaacgc caattggctg ttgccgtaaa acgcgcacgc
     8701 ttcctggctc tgctgcctta caccgaccaa cacaaataat tttggagatt gaatcatgca
     8761 aattattctg ttagaaaaaa tcggcggtct gggcaacttg ggcgacatcg taaccgttaa
     8821 aaacggctac gcccgcaact ttctaattcc cgcaggtaag gcaaaacgtg cgaccgaagc
     8881 gaatatgaaa gagtttgaag cacgccgtgc cgaattggaa gccaaacagg ctgaaatttt
     8941 ggcagatgcc cgagtccgtc aggaaaaatt ggacggtcaa accattaccg ttgcacaaaa
     9001 agccggtgtg gacggtcgcc tgttcggttc cgtcaccaat gctgatattg cggctgcaat
     9061 cgttgctgcc ggcatcgaag ccgtgaaagc aaatgtacgt ctgccgaacg gtcctctgaa
     9121 agccgttggc gagtacgaag tggaagtggc tttgcacact gatgccgttg ctaaaattac
     9181 cgttgccgtc gttgccgcaa ccgagtaatt gaaattatca gatgccgtct gaaaccctgc
     9241 cgattgtcgg aatgtggttt cagacggtat ttgttttgtt tttcgtatat tctgcaaaaa
     9301 tcgggatggt tgtttttata gtggattaaa tttaaaccag tacagcgtac ctggcttcaa
     9361 tatactcaat aggataggtc tcttcctaac cgaaaaaata attgtctttt cccttgcaaa
     9421 taattgaaat caacagatta ctaaaacaca gccaaaatat tcaattgaca aaaacttttc
     9481 tgattttgag aaatctgtga aaatcagggg attatttatt ttgaaaatgg tatcgggcat
     9541 aaaatttggc tgtgtttcaa tatggtgctg ataacatact ttgtttcaaa atgttcaata
     9601 ggataacttc ctgattgacc gaaaaataat ggccttttcc cttgcaagca gttgaaatca
     9661 acagattgtc aaaatacatc ctaaaaattt aataacttat atatatgatt gcaaaggtta
     9721 cggcttgtat gccggtcatt atggcggaaa taccgtttgg gaggcagccg catcgcgtgg
     9781 aatcggcacg gcaggcgatg cgccgatgcc ttcgggcgtg aaggcggggc aatcggcagt
     9841 ttggaaaaag gcaatggaat ctgtgggcag aagaagttaa gggtaaaagt cgggcgcacg
     9901 gtcaggttgt ctgtcctttt gtcggaacag gagactgggc agctttgtcc tgattaattg
     9961 cttgtgcaaa cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta cagcattttt
    10021 ccggcaatca aaagcaacat aatgccgaag gattttttga gtttggcaga agaaagtttg
    10081 tgggcggttt tgacaccgag cggggcaaag gcaatggttg ccgcgctgag gacggcgacg
    10141 gcgggcaggt aaaggaagcc cagtgaccct tcgggcaatc ctgcaatatt caggccgttg
    10201 agcagatacg atattgcgcc ggagagtgca atcggccagg caaggccgga tgatgtgccg
    10261 atggctttat gggcggggaa gccgcagtgg attaagaagg ggacggaaag tgaaccgccg
    10321 cctatgccga cccagctcga cattgtgccg aacagtgtgg aaaccgcagt cagtccgggc
    10381 agtccgggca gcgggcggga tgccgtctga gggtcggtat gcagtgtttt gaatgcgacg
    10441 gcggttaaaa acaggatgaa gaaaatttga agcccgaacg ctgggatata ttttgcggag
    10501 agtgcgccag cgaatacgcc gaataccata cccggcatca tcgtaaatac ggttttccag
    10561 tcgaccgcct gttttttgtg ctgccccagc atactggaaa aggcggtgaa gaccatgacg
    10621 gcgaaggatg tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa accctgcaaa
    10681 tcaagcaccc ataaaacgac agggacaatc agcgtgccgc cgcctacgcc gaacaggccg
    10741 gcaataaaac ctgccgcact gcctacggca agcaggatta agataatgtc ccaatgccac
    10801 atttaatccg cctttctgta ttttttccag aatcggcgtg ccgccataat gtagccggac
    10861 aggctgtatc cgagaaagaa caggaagagg acgagcgacg gttcccaagt gaccagaagc
    10921 agcagtaaga cggcaagcag cattccgaca aaggggactt gtctgcggat gttgatttct
    10981 ttaaaactcc aaaaagggat ttggacaatc atcgacaggc cggcaaacag tgtgatgccc
    11041 aatgcccacc agtggacggc ggggaatttt tcgacgctgt ggttgaccca aatcagcccg
    11101 acaatcagcg cggcggcagt cggactgggc acgccgataa accagcgttt gtccaccttg
    11161 ccgatgagcg tgttgaacag cgcaaggcgc agggcggcgc aggcgcagta gatgaaggcg
    11221 acggaataac cgattttgcc gaactgccaa agctgccatt tgtaggcaat cagagcggga
    11281 gcaacgccga agctgaccat atcggcaagg ctgtcgagct gctccccgaa cgcgctttgg
    11341 ctgttggtca gccgcgccac gcgcccgtcc ataccgtcca gcaacataga gatgaatacc
    11401 gcgatggcgg cggtttcata acgtccgtgc atggattggg tgattgcgta aaatgcggaa
    11461 aacagcgcgg cgatggtaaa ggagttgggc agcaggtaga tactgttttg acgcagggag
    11521 cgttgcgggg tgttggtcgg ggtttccata gctgaagtgt ctcacggacg tggagaatcg
    11581 ataggggatt ataacccaaa ccattatcga acaatacgcc ctttaggatg aagccctcgg
    11641 aggcggtttg tttttctgat ggcggatgct ttgcggggcg gtttgactct gcctgccacc
    11701 gggtgtgttg tttcggattt ttgccggatg gatattgttg ccgggcattt ctgggcttgc
    11761 cggaaggtat cggggggagg gcatcggaat atgcggcgca ccgttcaaac cggggcggcg
    11821 tttccctatt ttcagggggt tgcaggtttc aacaaattga aagatatttt ttgcatttcc
    11881 gaccgtatgc tgattttgaa gcgggataaa ttataggaca aggtttttcg cccgcggcaa
    11941 ggattgcccg caatgccgtc tgaaaacaac ggcttgccgc taaggcggcg gtatgcggat
    12001 ttgcgctgcc gaaatcggtt tgccataccc ggcggtgcaa tctgcgggga gggataagcc
    12061 gataaggcgg gacttatcct gttttttgtt tataatgcaa cagaatttgg acgaaccgtc
    12121 gggaatttat catgatgaaa aagatactgg ccgtatcggc actatgcctg atgactgcgg
    12181 cggcacgggc tgccgatact tacggctatc tcgccgtttg gcagaatccg cagaatgcaa
    12241 acgatgtttt gcaggttaaa accacaaaag aagattcgac gaaaagcgaa gcgtttgccg
    12301 agttggaagc tttctgcaaa ggtcaggaca cgcttgcggg cattgccgaa gacgagccga
    12361 ccgaatgccg gtcggtcgtg tcgctgaaca atacctgtgt cgcgctggca tacccgaaag
    12421 ccttgggcgc gatgcgcgtt gaaaacgccg ttgtgattac ttctccgcgt tttacgagcg
    12481 tttatcaggt cgcactcaac cagtgcatca aaaaatacgg cgcacaggga caatgcggct
    12541 tggaaacagt gtattgcacg tcttcttctt attacggggg gaactgtgcg ctctttgatt
    12601 caaaatctca aataaaacgg gaaatgccgt ctgaaagatg ttcagacggc atttctatat
    12661 cgacggtcag gattctttcg gatcgggcag caggctgttc aacataatgg caagtacggc
    12721 gcacaagccc acgccggcaa agctgaagct gcccaatttg agcgtcatgc cgccgatgcc
    12781 tgtggtcagt accgagctga cgatgaccag gtttttcggc agcatcaaat cgactttggc
    12841 atcaatcagg gttttgatgc ctaaagaagc aatcgtgccg aacagcagca gcataatgcc
    12901 gcccattact ggcatcggaa tggaagccaa aaacgcattg aatttgccga aaaacgccat
    12961 gcagacggca aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt tggtaatcat
    13021 caccgcaccc gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca gacccgcaac
    13081 gcatacgccc aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt tcgtatagtc
    13141 tttccccgtc acattgccga ttgccatgat gccgccgatg tgttcgatgg cgggggcgac
    13201 ggcaacgggc agcataaaca gtgcagcctg ccagttgacc tgaggcgttt caaaatgggg
    13261 aacggcgaac cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca tcagcagtgc
    13321 caaaacataa cccgaagcga caccgatcaa gataggaatc agcttcatca tcctgctgcc
    13381 gaaaaccgat acgatggcgg taacggcaaa ggtaaagccg gaaagaatca gcgaatcggt
    13441 atagtcgatg acctgtttgc cgtccgcctg acccattgcc atgctgcttg ccgccacggc
    13501 aacagacaga ccgatgacca tgatgacggg gccgatgacg accggaggca gcagtttgtg
    13561 taccgccgcc agtccgcgcc aacggatcag cgcggcaaac acaaaataca taaagccggc
    13621 ggcaaacagt ccgaacatgg tggaaggcag cccccattcg ccgacggagt agataatcgg
    13681 tgcgataaag gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg ttgtgatttg
    13741 gaacagcagc gttcccaagc ctgcgcccaa aagcgcaaga gccggattca gaccggtcag
    13801 caggggaacc agcaccattg cgccgaatgc cacaaataaa atctgcgcgc cggaaacggc
    13861 aagtttcagt tggttcatac catcttcttt caatcggttc aaacggcacg gattatactg
    13921 gtttgccgag ggtctgcgta aaaatacata tggcgggcaa cagatatgcc gtctgaaagg
    13981 caatgtctgt ctgttgcagg atgaaagcag gagaaatgga gtataatccg acactttcca
    14041 tattcaatcc gcaagccgtc cggcttcctt atttcagacg gcattttccg gtaatacggc
    14101 atgacagaaa aatcccataa aaaaacagca aaaggcaggg cgggaagccc gtccccaacg
    14161 tccgcacgca acaagaaagc cgacaacggc gcacggggca ataaagtttc cgagcggctc
    14221 aaagcggtca aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga acatgtcgtc
    14281 aaccttatcg gcgacgcact gtggctgatg ggtttggcgg caaccttgta tttggcgatt
    14341 tccctgatca gtttcgatat gggcgatccg tcttggtcgc acagttcgcc ggttgtggaa
    14401 gatgtcgcca attggggcgg actgttcggc gcgtatgttg ccgatgtcgg ctattatctt
    14461 ttcggctggt cgttctggtg gtggatagcg gctgcctgcg tcatgctgta taaaaatttt
    14521 cgtctgcacg caaaacagac ggaaaacgag gcatacaacc acaaaatcgc tgccgccgcg
    14581 ctgtttgtcc tgacggtctt cagccccgtc ttggagtatt ttgtgctggg cggaaaatat
    14641 gccgactccc tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg cgcagtgttt
    14701 gcgtggctgc tggggaaatc gggcagcctg ctgattatct tggttgttct gctgttgtcg
    14761 ttgtccctgc tggtgcagat ttcatggctg gaatttttga acggtgcggg cagggcggtt
    14821 caaaaccgcc tgagtgcctt atccggcaag gtcatggctt tgggaaaacg ccggccgaat
    14881 accaaaacag acggtgtcga tacccaaaat acacggcgca tggtaaaaga agccaagaat
    14941 attacggcca aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa atccgtcgcg
    15001 gtttccgtcg cgccgccgcc caaaattcag gtttctctgt ttgaagatga cgaacctcgg
    15061 caggcgggcg aataccacaa gcctacattg aacctattgc ggattcctga cagcgaaccc
    15121 gtcagcatca atcccgccga attggagcgc actgccgaac tgatcgaatc caaactggca
    15181 gaattcggca tcggcgtaca agtcgtatcc gccacatccg gtcccgtcat cacgcgctac
    15241 gaaatcgaac ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc gaaagatttg
    15301 gcacgctcta tgtcgctgca gtccgtgcgt atcgtcgaaa ccatcgcagg taaaaacacg
    15361 atgggcatcg agttgcccaa cgacaaacgc caagacgtga tgttgagcga aatcttgtcc
    15421 tcgcccgtgt ttgccggagc caaatccaag ctgaccgtcg cgctgggcaa agacattgcc
    15481 ggtacccccg ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc cggtatgact
    15541 ggttcgggca agtccgtcgg cgtgaacggc atgattatgt ctatgctttt caaagctacg
    15601 cccgacgaag tccgcttcat tatgatagac ccgaaaatgc tcgagttgag catttacgac
    15661 ggcattccgc acctgctctg tcccgtcgtg accgatatgc gcgaagcagg gcaggcgttg
    15721 aactggtgcg tcgccgaaat ggaaaaacgc taccgcctgc tttcccacgc cggtgtacgc
    15781 aatctggaag gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa gccgctgctc
    15841 aatccgttca gtctgaaccc cgacaacccc gagcctttgg aaaaactgcc gatgattgtg
    15901 gtcgttatcg acgaacttgc cgacctgatg atgaccgaac gcaaagccgt cgagcagcaa
    15961 atcgcccgtc tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt cgccacccaa
    16021 cgtcccagtg tcgatgtcgt taccggcctg attaaagcca acatcccgac gcgtatggcg
    16081 tttaccgtgc aaagcaaaat cgacagccgt accatcctcg accaaatggg cgcggacgaa
    16141 ctgctcaaat atggcgattc gctgttcctt cagcccggca gtgccgaacc gacccgcctg
    16201 caaggcgcgt ttgtttccga tgatgaagtc caccacgtcg tcgctttcgt caaggagcag
    16261 gcaccggcaa attatgtaga agggctgctt acaggcgaag cggcacagga aacggcaaat
    16321 atcgtcagcc ccaatgcaga cagtgacgaa ttgttcgatc aggctgtcgc ctatgttttg
    16381 gaaagcaaaa aaacctccat ttcgtctttg cagcggcagt tgcgtatcgg ctataaccgc
    16441 gcggcaaacc tgatggaggc actggaaaat gcaggtgtcg tttctccctc cgatcttaac
    16501 ggcagccgta aaattttggc gcacaaggac catttatagc ccatatcgca aaatgccgtc
    16561 tgaacggtgg aattggcgtt tcagacggca tattatgttt caggcgaaac attgtgatat
    16621 acttgccagc taaaatttcc cctttgcggc aatgcggttc aaatatcgta ccgttgcgct
    16681 gtttgcttcc ccatgtaggg aagaaagttt atcattttat caacacaaca aatttaaggg
    16741 cttatgatga gcgtaactgt tgaaacttta gaaaatctgg aacgcaaagt agtgttgtcc
    16801 ctgccttggt ccgaaatcaa cgcagaaacc gataaaaaac tgaaacaaac ccaacgccgt
    16861 gcaaaaatcg acggtttccg tccgggtaaa gcacctttaa aaatgattgc ccaaatgtac
    16921 ggtgcgagcg cacaaaacga cgtgatcaac gagctggtgc aacgccgctt ccacgatgtt
    16981 gccgttgccc aagagttgaa agtggcaggc ttcccccgtt ttgaaggcgt tgaagaacaa
    17041 gacgataaag aatctttcaa agttgccgcc atttttgaag tgttccccga agtcgttatc
    17101 ggcgatttgt ctgcacaaga agttgaaaaa gtaaccgctt ccgtcggtga tgccgaagtg
    17161 gaccaaaccg tagaaatcct gcgcaaacaa cgcacccgct tcaaccatgt cgaacgcgaa
    17221 gcccgaaacg gcgaccgcgt catcattgac tttgaaggca aaatcgacgg cgaacctttt
    17281 gccggcggcg catccaaaaa ctacgccttc gtattgggcg caagtcaaat gctgcctgaa
    17341 tttgaagccg gcgtagtcgg catgaaggcg ggcgaaagta aagacgttac cgtcaatttc
    17401 cctgaagact accacggtaa agacgttgcc ggtaaaactg ccgtgttcac cattacgctg
    17461 aacaacgttt ccgaagcgac tctgcctgaa gtcgatgcag attttgcaaa agccttgggt
    17521 attgcggatg gcgacgttgc caaaatgcgc gaagaagtga agaaaaacgt aagccgcgaa
    17581 gtggaacgcc gcgtaaacga acaaaccaaa gaatccgtaa tgaacgcgct gctcaaagcc
    17641 gtagagctga aagcacctgt tgctttggtc aatgaagaag ccgcacgctt ggcaaacgaa
    17701 atgaaacaaa attttgttaa ccaaggtatg gctgatgctg ccaacttgga tctgcctttg
    17761 gatatgttca aagaacaagc cgaacgccgc gtgtctttgg gtctgatttt ggccaaactg
    17821 gttgatgaaa acaaactgga accgactgag gagcaaatca aagccgttgt cgccaacttc
    17881 gcagaaagct acgaagatcc tcaagaagta atcgactggt actacgcaga gccttcccgc
    17941 ctgcaagccc cgacttcttt ggcgatagaa agcaacgtcg ttgatttcgt tttgggtaaa
    18001 gccaaagtaa acgaaaaagc tttgtctttt gacgaagtga tgggcgcgca agcctgatta
    18061 tcctgaaaat gccgtcttag ggcagttttt gaaagcaccg aagccgcttt taaagcccgc
    18121 tttggtgctt ttacatcatg aaaggagacg aaatgtcttt tgataactat cttgtcccta
    18181 ccgttatcga gcagagcggt cgcggtgagc gtgcattcga tatctattcc cggcttttga
    18241 aagagcgcat cgtattcttg gtcggcccgg taaccgacga gtccgccaat ctggtggttg
    18301 cccaactgtt gtttttggaa agtgagaatc cggataagga tattttcttc tatattaact
    18361 cgccgggcgg ttcggtaacg gccggtatgt cgatttacga caccatgaat tttatcaagc
    18421 ccgatgtatc gactttgtgc ttggggcagg cagcaagtat gggcgcgttc ttattgtcgg
    18481 caggcgaaaa aggcaaacgt ttcgccctgc ccaacagccg gattatgatt caccagcctt
    18541 taatcagcgg cggcttgggc ggtcaggcat ccgatattga aattcacgca cgcgaactgt
    18601 tgaaaatcaa agaaaaactc aaccgcctga tggcgaaaca ttgcggccgc gatttggcag
    18661 atttggagcg cgacaccgac cgtgataatt tcatgtctgc cgaagaagca aaagaatatg
    18721 gtttgatcga ccaagttttg gaaaaccgcg cttctttgca gctttaaacc ttgtgaagta
    18781 gacaaatgcc gtctgaaaac agttcagacg gcatttgtct tatagggact gtattgggat
    18841 gattttgttc tggttgcgtt tggagccgtt ttcgccgtag ttggttttga catactcttc
    18901 aacaatagcc aaaaattccg ttgcaatgtt gtcgcctttc agcgttactt tgcgttcacc
    18961 atctacataa acaggcgcga cgggtgtttc ccccgtaccg ggcaggctga tgccgatgtc
    19021 ggccaatttg ctttctccgg gcccattgac aacgcagccc attacggcaa cgttcaggga
    19081 ttcaacccca ggataaaggg tacgccatat agacattttt tggcgcaggt aattttgaac
    19141 atcttgtgcc agctcttgaa atacggtact ggtggtacgc ccgcagccgg ggcaggcggt
    19201 aaccatcggc gtaaacgaac gcaatcccat agtttgtaaa atctcttgcc cgacgacgac
    19261 ctcctgagta cgcgggctgc caggttccgg agtcagtgaa atgcggatgg tgtcgccgat
    19321 tccttcttga agcaagacgg ataatgccgc cgttgatgcg acaatgcctt tgctgcccat
    19381 accggcttcg gtcaaaccca aatgcagcgg ataggcgcaa cggctgccca gttcgcggta
    19441 aacctgaatc aaatcctgaa ccgcgctgac tttgcacgac aggatgattt tgtcttcggg
    19501 cagtcccaat agaacggctt tttcggcaga ttccaaagcg gagacaatca gtgcttcctt
    19561 cgtcacttct tcgggcggtt tcggcgcgga agaagcgagg ttggcatcca tcatacgttt
    19621 ggcgaggctt tgatccaaag aaccccagtt tacgccgatg cggacggctt tatcgttttc
    19681 agcagcagtc cgaatcataa aggcaaattt ttcatcgcct tttacgcctt tgccgacatt
    19741 gccgggattg atgcggtatt tggacaatgc tttgccgcat tctggaaatt ccgccaacag
    19801 gcgttcgccg ttgaagtgga aatcgccgat aagcggtgtg gcatagccca tatcgtccaa
    19861 gcggcggcgg atttcggcaa ctttggacgc ggcttcgggg ctgttgacgg taatacgcac
    19921 catttcggat ccggcgtcgc tcaattcctt aatctgcaat gcggtggctt ttgcatcggc
    19981 agtgtcggtg ttggtcatag attggataac gacgggtgct tctgaaccga cggtaatatg
    20041 atcgatgcgg acttgatgcg tcttgcggcg ttggagtgtg ttcatatggt ttgaatctgt
    20101 ttattgaccg atgaggacgg tttgcaattc ttccgagtag gggaaattcg cctgcaattg
    20161 tgcttcatat tcgtatgccg cctgtgcgtt gccgagggct ttggcaattt tccagcctag
    20221 cagcaaatca tcggcctgaa ggacttctac cctgctttgg tattttttaa agtagtaatc
    20281 ggcatcgccc aactgcccgg ccagcatttt ggtgcgcgcc agttctttaa atgcgggtgg
    20341 gaactgcggc tgggcggcga gggaacgttt caaataggct tccgccaatc cgaattgccc
    20401 ctgttttgcg ctgcatatgc ctttattcag gttggcaata taaggggtcg ggtaggtggg
    20461 gtcggccagg gctttgtcga aatatgccat agattcggca gggcggttga gcctgccgca
    20521 caggaaccag ccgtagttgt tgttgatttc ggcactgtcg ggtttgatgg agagggcttg
    20581 ccggaaactt tcctgcgcct tgtcgttaac tttcaggtat tgatagattt cggcacggac
    20641 cagccaggca agctcgtttt tagggtctga tttcaaggcg tcttcaatac ttgccgtcgc
    20701 ctgacggtag tcctgaccgc gcatatattc cattgccaac tgggttttga tattggaaac
    20761 ctgattggct ttttctgccc gcgaggggcg gtaggaagtg ctgcacgcgc ccaaggcaag
    20821 gacaagtaat aaagagattc gtttggatgg cttaaaaggc ataattaccc ctgttgtccg
    20881 attaaaatct gctgccattt ttgttggcgg cgcgttttat cctgaacctg ccccgccaac
    20941 tgtccgcagg cggcatcgat gtcgtcgccg cgcgtttttc gtacggtaac gacaaatcct
    21001 gcctgctgca aaatatcgcg gaacacacgg atgttctcat tgctggagcg ttcgtatccg
    21061 gagtttggga agggattgaa cggaatcaga ttgaacttgc agggaacatc tgtgaccagt
    21121 tcgatcagtt cgcgcgcatg ttgcgcctta tcgtttattc cgtccaacat gacgtattcg
    21181 aaagtgatga aatccctggg tgctttgacc agatagcgtt ggcatgcggc catcaattct
    21241 ttcaagggat attttttgtt caacggtacg atttggttgc ggacttcgtc attggaagcg
    21301 tggagggaaa ccgccaaagc caccggcatg acatcgcgca acctgtccat ttggggaacc
    21361 atacccgaag tggaaacggt tacgcggcgg cggctcaaac cgtagccgtg gtcgtccagc
    21421 atgatgctta aggcggtaac gacattgtcg aagttcgcca tcggctcgcc catacccatc
    21481 atcactacat tggaaattac gcgttcgttt ttcggtgtaa cacccatcgc tttgtttgcc
    21541 caccacaatt gcccgatgat ttcggcagca gtcaaattgc ggttgaagcc ctgccggccg
    21601 gtcgaacaaa atgtacattc caaagcgcag ccgacttgtg aggaaatgca gagcgtgccg
    21661 cgatccgatt cggggatgaa gacggtttcc accccgttgc ccgtaccgac atccaaaagc
    21721 cattttcgag tgccgtctga agatttttga gacatcatca gcttgggaat ttcgatgcct
    21781 gcctgttcgt tcagtttatg gcgcaacgat tttgccaaat cggtcatttc gttaaaattt
    21841 tgcgcgccgg attggtgcat ccaacgcata acctgtttgg cacggaaggg tttttcgccc
    21901 atatcggcaa aatgtcgggt cagcccttga aggtcgtagt tgagcagatt ggttttcatg
    21961 tgtttgtgtt tttcttaaat caaggcttca aataaaaaat tgcagggcag gcaagtttga
    22021 tttgaagcct tttgcaacag agtcttcaga cggcatggat atattttatg ccgtctgaag
    22081 aggagttggg cggtgtattg tatcaacggg ggcagatttc ggtttggctg aaaaagtagg
    22141 caatttccaa agcggcattt tccacgctgt cggaaccgtg tacggcatta atgctgaccg
    22201 aagtggcaaa gtccgcgcgt atcgtgcctt cggcggcttc ggaaggatta gttgcaccca
    22261 tcagttcgcg gtttttcagg acggcgtttt caccctctaa tacctgaatc ataaccggac
    22321 cgccggtcat aaattcaacc aatccggcgt agaaggggcg gtctttatga accgcataaa
    22381 attcttgcgc ctctttgagg gtaagctgct tcattttggc ggcaacgatt ttcagaccgt
    22441 tctcctcaaa gcggctgtat attttgccga taacgttttt gccgacggca tcgggtttga
    22501 tgatggagat ggtacgttca atcgccatgc tatatcctta tttttactta agaagaatca
    22561 aatcggtatt ctatcaaaaa aataacttat ccgcctgaag ccttgggaga tagggtcagg
    22621 tgcggctgtt ccaaataaac ttcggactgc atttcgacca tccggctggc ggtgcgggta
    22681 aattcttcgg caaaatcgcc ttccgtatag atatggttga catctaccgc gccggtggca
    22741 cacagtttga cccggaaatc gtagagtacg tcaatcagcc aagtcagccg ccgcgcctcc
    22801 gccttttctt gcggtgagag ttgttccaaa cctgaaataa aaaccatttc ataatgttcg
    22861 gccaaataca gatagtcgga ctgtgagcgg gggccgaagc acagtgcgcg gaaatcaaac
    22921 catatggcac ggccggactc ggctttgtgg ggaatctccc gaccatggat ggtgctgatg
    22981 ccggggttca aatcggtaat gcctgtcatt tctttgaaca gttttgccag ttttgcctca
    23041 ttttcttcat tggcaggcgt aaagaaaatc tcggcggggc ggagggtacg cagtcggtag
    23101 tcttcaccgc cgtcaacgtt taagacggtc aggctggact cgatgagcgc gattgtggga
    23161 agaaaactgc tccggttttg accttgcggg tagagttcgg aaggcgcgta gtttgaagtc
    23221 gccaccaaaa caacgccctc gttaagcagg ttttccagca gacggcctaa aatcattgca
    23281 tccgcaatat cgctgacatg aaattcgtca aaacacaata cgcgggtttc tttggcaatc
    23341 tcggcggcaa cggatttcaa cgggttgctt tcgcttttca gggttttcag cctctggtgg
    23401 atttctgcca taaaggcatg aaagtggacg cggcgtttgc ggcggtaagg gaggcagccg
    23461 aaaaaagcgt ccatcagaaa gcttttgccg cgtccgaccc cgccatagaa ataaagccct
    23521 ttggggactt gcggggaacg caaactcctg cctaaaaaac ggtttctttt gcgtttgaac
    23581 atcatcaatt cggtccaaag ccgatcgagg tgttcgatgg cggctgcctg cgcgtcgtcg
    23641 cggatgaagt tgggcagttg tgaggcagcc tgataccaag tcagcgggct gtgattttca
    23701 aacggcgggg ctttaaaaag ttggtctcta ttactcatat ttaaccttgt tatacttctt
    23761 gcggcggaga aagaaaatgc ttaccgtcgg tattataaat gatttccttc aaacttggcg
    23821 ttttccgtgg aaaagaataa tgacgcgcac aagggggggg tatgagcaaa ggcggcgttt
    23881 caatatggtg cagatatgag ctcaaatgcc ttttatagta gattaaattt aaaccagtac
    23941 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
    24001 tttttgttaa tccactataa tccgtagagt cggcttcttc tggctcagac ggcatacgtt
    24061 tatgccggca cttcttaccg ccctcgtgcc ggctatccca aaactgcttt gaacactgaa
    24121 cgctctttgg tttacttcaa aaacgcggcg tgataagcaa tatgctcgcc aataaaactg
    24181 gcgatgaagt aatagctgtg atcgtagcct ttatggaaac gcacatcgac cggctggttt
    24241 gccgcacggc aggtttcgat aaaatcttcg gtacgcaatt gtgtcggcaa aaactcatct
    24301 tccaagcctt gatcgatgcg cataccttgc actttatagc cttgttgaat gagtgagttc
    24361 gcatcatatt gctgccattt ttcacggtct ttccctaaat aagcagtaaa ggctttttct
    24421 ccccacggca cgaggcttgg cgataaaata ggcgaaaagg cagaaacact ttgataacgt
    24481 tcctgattcc gcagcgccaa taccaatgcg ccgtgtccgc ccattgaatg ccccataatg
    24541 gaacgtttgc cgttggtagg aaagtgtttc tcaatcagac ggggcagctc gttcaaaatg
    24601 taatcataca tttgataatt cgccgcccaa ggctgttcgg tcgcattcaa ataaaagcct
    24661 gcaccctgtc ctaaatcgta agcatcatcg ttcggcactt gctctccgcg agggctggta
    24721 tcggaggcca ccacaattac ttgatgttct gccgcataac gctgaaagcc tgatttggta
    24781 atgaaatttt gttccgtaca cgtcaagccg gaaagccaat aaatcacacc aagcggtcga
    24841 ttttctggat tatttggcaa atagacggca aatttcattt cgcattgaag cgtttgggca
    24901 ttgtgtgccc aaacttgttg cgaaccgcca aaaatttgat gttgttcaat cagtttcatc
    24961 gcatacctta gtagtgaata acggcgcgga tcgatttacc ttcgtgcatt aactcaaagg
    25021 cttcattgat ttgatcgagt gtcattgtgt gggttacaaa cggttctaac tcaatgtcgc
    25081 ctttcattga atcttccacc attttcggca gttcagagcg gcctttcacg ccaccaaatg
    25141 ccgtgccttt ccacacacgg cctgttacca actggaacgg acgagttgaa atttcttgtc
    25201 ctacacctgc tacgccgatg ataatggatt gtccccaacc acggtgtgca ctttctaatg
    25261 cttgacgcat tacgtttaca ttgccgatac attcaaaggt atggtcaatg ccccatttgt
    25321 tgatgtctaa caacacatct ttgatcggtt tatcgtaatc gttcgggttc aaacagtccg
    25381 ttgcaccgaa ctgttttgcc aactcgaatt ttgagggatt ggtatcaatg gcgataatgc
    25441 ggccggcttt ggcttgacgc gcaccttgca caaccgccaa accaatcgct cccaagccaa
    25501 acactgccac agagtcgcct tcttgcactt ttgccgtatt atgcaccgcg ccaatacctg
    25561 tagtaacgcc gcagccgagc aaacatactt gttcatggtt cgcttccggg ttaatttttg
    25621 ccagtgaaac ttctgcaaca actgagtatt cactgaaagt cgaacagccc atatagtgat
    25681 agatcggctg accttgataa gaaaaacgcg tcgtgccgtc cggcattaag cctttacctt
    25741 gtgtatcacg cactgagacg cacaagttgg ttttacctga acaacaaaac tcacattcgc
    25801 cacattcggc ggtgtaaagc ggaatcacgt gatcaccggg ttttacgctt gacacacctt
    25861 cgcccacagc aacgaccaca cccgcacctt cgtgtccaag caccacaggg aatacgcctt
    25921 caggatcgct tcctgataac gtaaacgcat cagtatggca cacgccagtg tgggtattgc
    25981 ggattaacac ctcgccttta cgcggcattt ctacgtcgat ttccacaatt tgtaagggtt
    26041 gatttggggc gaatgccacc gccgcacgag atttgatggt tgaatcggct tgtttcattt
    26101 ccattgtctt tcctcttaat tcattgacaa agttgccttg cctgagcaac atagcaacag
    26161 tttacacttt agagttaact ctaaagcaag tgttttttac tgtttttgtt tagaaggagg
    26221 gaaaatgact tatactactg ccaaagccgc cgaaaaaata ggcatctccg cccacaccct
    26281 acgtttttac gacaaagaag gtttgttgcc caatatcgga cgtgatgaat acggtaaccg
    26341 ctgttttacc gataacgatt tgcaatggtt gggcttattg caatgcttga aaaatacggg
    26401 gatgagctta aaagacatca aacgctttgc ggaatgtacc gtcattggcg acgataccat
    26461 tgaagaacgc ctttccttgt ttgaaaatca aatagaaaat gtgaagtgtc aaattgccga
    26521 attaaaacgc tatttagatt tgcttgaata caaattggcg ttttaccaaa aagcgaaagc
    26581 attaggctcg gtaaaagctg taaatttgcc gcaaattcct gaaacggctt agttttagtg
    26641 caataagcag gatttttgtc ctgttcgtaa tagaaatcaa aaatttgttg tttaaaaagc
    26701 ggattgtaag caatgatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
    26761 acagtgtaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga gaattgttct
    26821 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaaaaaatcc
    26881 acgctctaat ccgttgcttg cttggctcaa ggtattgaag caacggatga agttaaacca
    26941 cagaaaaaac gccgcgtaac agcggcgttt tgatgctaag gtttaattgg cattttcttt
    27001 taaagccaag tcgacccgct cgcgcaattc tttaccgggc ttgaagtggg gtacatgttt
    27061 ttcaggtact tccacacgct cgccggtttt gggattgcga ccgatgcggg caggacgatg
    27121 gttcaaatcg aagctgccga aaccgcggat ttcgatgcgt tgacctcggg caagcgatct
    27181 agtcatggtg tcaaccaaga cttttacgct gtactctacg tcttttgcca gaagatgcgt
    27241 gccgtttttg gcggcaaaca cttctgccaa acgaaccatt aactcagact ttgtcatgtc
    27301 tgcaacctta ttcttgttcg ccggagagtt tggctttcag caggtcgccc aagctggtgg
    27361 tgccggcatt cgcattggcg gcggcattga cggagttcag tgcttcgcgg ctttctttgg
    27421 catctttggc tttaacggaa agtttgatgc tgcggttttt gcggtcaacg gtaacgatga
    27481 cggcttcaac ttcgtcgcct tctttcagtt tggtggtcaa atcttcaacg cggtcggctg
    27541 caaattcgga agcaggcagg tagccttcta cttcgtcaga cagggcgata acagcacctt
    27601 tggcgtcaac agatttcacg gaacctttaa ccaaagaacc tttgtcgttc acgctgatga
    27661 agttgccgaa cggatcgcct tccagttgtt tgatacccaa ggagatgcgt tctttttcca
    27721 cgtcgattgc caatacgacg gcttcgactt cttcgccttt tttgtatttg cgtacggctt
    27781 cttcgccgga ttcggtccag gacaggtcgg acaggtgaac caaaccgtcg atgccgccag
    27841 gcagaccgac gaatacgccg aaatcggtaa tggatttaac cgcgccggag attttgtcgc
    27901 ctttgttgtg gttggcggca aattcttccc aaggattggc ttggcattgt ttcataccca
    27961 aagagatacg ccggcggctt tcatcgattt cgaggatcat tacttcgact tcgtcgccca
    28021 attgtacaac tttgctcggg tgtacatttt tgttggtcca atccatttcg gaaacgtgca
    28081 ccaagccttc gatgccttgt tcgatttcaa cgaacgcacc gtagtcggtc aggttggata
    28141 ctttaccgaa caggcgggta ccttgcgggt aacgacgggt caggccgctc caaggatctt
    28201 cgcccagttg tttcataccc agagaaacgc gttgtttgtc ttggtcgaat ttcaggactt
    28261 tagcttctac ttcttgaccg acttccagaa cttcgctcgg gtgtttcaca cggcgccatg
    28321 ccaagtcggt gatgtgcagc agaccatcga tgccgcccaa gtcaacgaat gcaccgtaat
    28381 cggtaatgtt tttaacgatg cctttgatga cggagccttc ttgcaggttt tccagcaggg
    28441 ctttgcgttc ttcacccaag gtagcttcca gaacggcgcg gcgggacaca acaacgttgt
    28501 tgcgtttttt gtccagtttg atcactttga attcaatttc tttaccttcg aagtgagaag
    28561 tgtcttttac aggacgtacg tcgaccaaag aacccggcag gaatgcgcgg atgctgctaa
    28621 tcataacggt caggccgcct ttgactttgc cgttgatgat gccggacagg atgttgccgt
    28681 tttccatggc ttcttccagg gcaatccaat cggctgcacg tttggctttt tcgcgggaca
    28741 gtttggtttc gccgaagccg ttttcgacgg attcgatggt aacggtaacg aagtcgccga
    28801 ctttaacttc aatttcgcct tgagcgtttt tgaattcagc tacatcaatc agggattctg
    28861 atttcagacc tgcgtttacg gtaacgaagt tttggtcgat tgccactact tcagcggtaa
    28921 tcacctcacc cgggttcatt tcttgcaggg taaagctttc ttccaacagc tgagcaaaat
    28981 tttccataga catatataac tcttttcggt acaccgccaa ggggtgcggg gtgggttggt
    29041 ggatatctgc cgtccttggc agggcagacg ggtacataag gtttcagacg gcatatgggg
    29101 gatttttatg ccgtctgaaa cgtaatttgt gcgattatac ctgaaaattt aaacttcacg
    29161 ataccaatca agtacttttt ttacagtttc ttctatagtc aggcggcttg tgtccaaaag
    29221 cagggcatcg ggctgttgtt tcaggggggc aaccttgcgg tttcggtctg cctcgtctct
    29281 ggcttcgatg tcggacagga tgcgctcgaa tgccaaacct tcgcagggga tgccgatttg
    29341 tttggcgcgg cgttcggcac ggattttgga ttctgccgtc aggaagattt taagttcggc
    29401 ttgggggaag acgaccgatc cggtgtcccg tccgtcggcg accagtcctt tttcggtcag
    29461 aaaatcgcgt tggcgttgca gcagggagtc gcggacttta ggcaactgtg cgactgcgga
    29521 tgcgcccatg ccgatggctt ctgtccggat gccgtctgaa acgtcttcgc cgccgagcag
    29581 gatgcggctg tctgaaaata cggcgggcag tttttttgcc agttcggaaa cgttttcttc
    29641 atcgtgccat cccacgcctt gtttttgtgc atatagggcg gtcaggcggt agagtgcgcc
    29701 ggtatcgaga taatcgtatc ccaatgcggc ggcaacgcgg gcggcaaccg tgcctttgcc
    29761 cgatgcgccc gggccgtcga tggcgatgac tttttgtctg ttcataaggg ggattcctga
    29821 tggtttgggg tatgggtttt gccgtctgaa ggatgtgttt cccgttgggg cggattctac
    29881 ctgttttaaa ggacgattgt ctaagcagac ggaacgccgc ctgcccggaa atatccgaca
    29941 gaagccggca agccgattcg gattccggcg ttgcgggtat tgtggcgggc atcaatgtaa
    30001 tgccgtctga agtcagtatg gctttcagac ggcattaatc ggtgtgaatc agaaaaggtt
    30061 gctgacgaaa atcagcagaa tcaccaacgg cacaagatat ttcacataag caaaccaaat
    30121 attgaccgtc gtatggttgc ctttataaag caattcttcc ttcgcttcat ccttcatcac
    30181 gaaaccgaca aacaatgccg agccaagcgc ggtcagcata aacaagatgt tgccgctgat
    30241 gtagtcgaag gcatcgaaaa tatttttgcc gaacacggaa acgtctttcc acgggccata
    30301 gctcaaaata gacgggatgt tgccgaaaac gtagatggcg gccaatacaa tcgtgattgc
    30361 ggcggtacgg cggattttgg ttttttcctg aatggtcgta atcaatactt cataaatcgt
    30421 cagcgaagtc gtcaacgcgg caatcaggag cagcgagaag aaaatgacgg cgaacacaga
    30481 tcctgcccac atatgcgaga acacaatcgg caaactttgg aataccaaag tcgggccgga
    30541 atcgggggca acgccgaagc tgaagagcga cgggaaaatc ataaagcccg caagtatggc
    30601 gatgatggta ttggtaattg ccgtgataac tgccgtctga accagatttt cgtttttatc
    30661 caaatagctg gacaaggtaa tcatcacgcc gaaacccagg ctcagggcaa aaaatacctg
    30721 ccccaaaacg aagacgaaca gttcggcggt aatcttgctg aaatcaggtt tcagatagaa
    30781 agcaacccct tccattgcgc ccggaagggt aacgttgcgg acgaccatcg cgattaggaa
    30841 caaaaacagc agcggcatca gctattttgc cgctttttca atgccgccga taacgccttt
    30901 gaccaaaatc cattggttca cggcgacaaa aagcagcgta taaaacgcaa tttcccaagg
    30961 gctgtttcaa tgtgttcggc aaagaagcct tttgtaacca caccgtcgat ggggctggaa
    31021 atattcaaat ttcctccaat aatattaacg atatagctga ttacccagcc gccgagtgcc
    31081 atgtaataag ccatgatgcc gaacgcgccg agcagcccca tccagccgac cagtttccaa
    31141 attttagcaa tgggtttgcc gttcatcgga ccgccgaacg catccagcgc gttcacgcct
    31201 ttgcgccgtc cgatgacgtt ttccaccaaa atcatcggga tgccgataac gagcatggcg
    31261 atacagaata aaaacacata cgcgccgccg ccgttttcac cgaccaaata cgggaaacgc
    31321 cacgtcgcgc cgaagccgac agtcgcgccg gcaacagtca ggatataggt taatcggctg
    31381 gatcaggttt gacgattggt atttgaaggg gaagacatat tgaaaccgtg tccgattgag
    31441 ataaagcgga aattctacac gcgtttttta acaggaattg actgattgtt tttcaactgt
    31501 ctagtgattt tccatgtaaa aggcatatat ttcagtgagt atttctgatg agtataaccc
    31561 gatgaggaag aatcggagtt tataaataga ttaatttgtt attcttctac atcggtgtat
    31621 ggaaatgaaa ttttgttaat cacattaatg atagcatttt aataatgatg aaagagaggg
    31681 aatttgaaat aaaaaaccat ctatacaagg ggagaagtat agatggcaaa acacattacg
    31741 gggaaaacgt cttactcata agcctgcttg aacaggcgtt actcagacaa atggattata
    31801 tcgtaaattg attttttgcg ttaaattggg ttaaaccata tatttaatga gtatgtggat
    31861 gaatataaat ggaattcttg gatgttattt tagaatttcg gacaatcgat aaatttccat
    31921 tcgccgggtt tcaggttttc tgtttccaaa tgtgcgaatc gccggcgatg aaggtgttgc
    31981 acgcggttgc ctgcggcggc gaccatacgt ttgacttggt ggtattttcc ctcggtaatg
    32041 gtcaggagca atgcggacgg gctttccaat acggcatcgg cggcgtaaac cgtttcgttt
    32101 tcgtcgtgga gcaggacacc gtttttcagg gtttcgcaaa gcgtttcgcc ggcagggtgc
    32161 ttgagtgtaa cttcgtatag cttgggaatt tttctgctcg gcgaagtcag gttgtggttc
    32221 agtttgccgt cgttggtaat cagcaatacg ccggtcgtat ctgcatccag cctgccgacc
    32281 gcctgcatat cgatgttccg catattgtcg gggaacaggc tgaatacgct gcggtagtgc
    32341 ttgggtttgt gcgaagtttc gtaatcttca ggcttgttga gcatgatgta gaaatagggt
    32401 tcgggaacga cggttactgc ttccccgtca atatccaacg tttcgacgga tgaggaatcg
    32461 atgtctgcat cggtgtcgtc catgcaggtt ccgttgatga aaacataacc gccggcaatc
    32521 agccattggc actgcttgcg gcttcctatg ccttgatatt gcaggtattt gataagtttc
    32581 atgatggtat gggaaatgtg ggatgaaaaa gacaggactg agaaagtcct gtcatgtgcc
    32641 gtatccgaat cagcttccaa tcagtttgac cctctgtccc ggattgatgg tgttcaggtt
    32701 gggattgagc cggcggatgt cgtcgatatg gatattgaag cgcgcggcga tacttttgaa
    32761 ggtgtcgcct ttgcgcgcgg tgtaggatac tttttcaata cgggtttggg caggggctgc
    32821 ctgcgccagg cggagtacct gtcctttttg gatggtgttg cctttgatgt tgttggcgac
    32881 aatcaggtcg gctacactga cgttgtagcg tttggcaatg ttgaacaggg tgtcgccttc
    32941 tacaacgcgg tggatgctgg catggagcgg ggagatttcg gcttcgccgt cggcagcttt
    33001 ggctacgcgc gtttccaaac gtacccggcg tttgccgtcg gcggcaacgg tatgttgcgg
    33061 agatgaggaa atgttgtgtt cggcaactgt gtcaggcgtg ccgatgacgg cagagatggt
    33121 ttcttcagcc tgtcggcgca ggttgtttcg ggcaacaagc tgcatgagtt cgtctgccgc
    33181 ctgcatgtcg ttttggggga cgatctgcgc gagtgttgcg gtttgggttt cagacggcat
    33241 ggcggtctgt ttttcggttt gaggttgcgc tgcggctgtc gcaagggcag gttcggcaat
    33301 acggacaagg ggatcgggtt ccgtacggac ggttttctgc ggcaaaggtg cgacggtaat
    33361 gtccgctgtc tgtgcggcgg cgggtcggat tcgggcaatg ccgacgttca ccgtgcctgc
    33421 cggcatattg gaacggtagg tgtcgggtgt attgtcgatg tcgatggaaa cgacggattc
    33481 cgatgccgtc tgaagggttt tgccgttctt ggcgacaagg atgctgcgtc ctgcgttgac
    33541 aaggttgccg ttcaggttgt tgaggcgttt gatgtcggca atgctcatgc cggttgccgt
    33601 cgagatgtcg gacaggctgg ttttggcggc aggcgtatag acttcccatg aaaacaggct
    33661 gtcgggtgcg gcgttgaggt agttgctttg gaaggtttgt acggacgcga caggaagcag
    33721 cagtttgcgt ttgcttttgg ggataaacgc ggggacgttg aatgcggggt ttagggcgag
    33781 cagctcgctt tgcgtgatgc cggcaagccg ggcgatggct tcgttgtcga gcggacgatc
    33841 cggttcgact gcctgaaaat agggtttgtt gtctatgtcg ctgatattca tgccgaaaga
    33901 ttggggagtg gcaataatgt tgcgcacggc gagcagcttg gggacatagt tgcgcgtttc
    33961 gttgggcata cgcaggtttt cgtaggtcgg ttcgagccct tgggcgcggg cgcggttgat
    34021 ggcgcgtccg acgttgcctt caccccagtt gtaggcggca aaggcaagcg gccagtcgcc
    34081 gaacagtcca tagaggtatt gcagatagtt gagtgcggca tcggtggcgg cgtaaacgtc
    34141 gtgcctgccg tcgtaaaccg gtgttttttc caggccgtaa tgcctgccgg tagcgggcat
    34201 gaactgccac aggcccgatg cgccgacgtg tgatttggct ttggtgacga acgcgctttc
    34261 gatgaaggga agcagggcgg cttcggcggg catattgcgt tttttgactt cgttggcgat
    34321 atggtacata tagggtctac tccggttgat gaccctgttg aaatagctgt ggcttgcgat
    34381 gaatttgctt tcgtggcggc gtaccagttc gggattgact tcgcccatcc ggaagccttg
    34441 gcgcagctcg ctccacaggc tgccggattg gaaatattgt tttgtcgggg gcaggtcgag
    34501 tattgaagag tttaagcgca taatcgccaa accgatttgg tgtgatgagg tgttttgggc
    34561 gtataggaaa cccggacaaa cggacagacc tgacgcggtc agggcgatgg ttttgagttt
    34621 ggacatgatt tatatcggcg gaataaacat attttatcgt ccgatggtac tgtttgactg
    34681 aaaaagcgtc aagttttaca ggaactttat gcggattcgg aaatgggcgg ccgatgtgtt
    34741 ttttgacaga aatcctgttt ggaatagaat atcggcaggc aatctgtttg aaacggacgc
    34801 aatatggatg catggtttga agatacggcg atggggcggt atgttgcaaa attggaacag
    34861 gatttcttcg ggcggtatct agattcatac cgtttttcgg gaatgtgtgc ggttcaggtg
    34921 ggcggtccgt ggctgagcct gtctgaagat gttgtctgtg tgccgcgaga tatgtcgatg
    34981 tcggcggaga atatggcttt ggcggatgtt tctgcggata tgctgctttt tccgcatacg
    35041 cttgaaggcg gtatcccttc gcaaatcctg tcggaagcgc accggatatt gaaaccgtgc
    35101 ggacgcttga tgctgacggg tttcaatccg tattcgctct ggggattcag ccgttggttt
    35161 gacggcgaac gcctgccgga aaaacggttt tgtctgccgc tgcccgagtt gaaaaggcag
    35221 cttgcggatg tcggtttcga tattgaattt gggaaattta tggtgtatct gccgcccgtt
    35281 tcgtcgatcg ggcaaatacg cttttggcgg tttatggaaa aggcgggcga ccgttggtgg
    35341 ccgcaatgtg ccgcagtgta cgctttggtt ttggtcaaaa gggcggcggg cgtaacgccc
    35401 ctgcccgcgt gggacgggta tttgggcggc aaagcccttg cggcgggtgc ggcaagggtt
    35461 gcggattagg gacagccgtc tttcagacgg catctgtcag aaaaaatgtc gggaggggtt
    35521 atgccggaat tgccggaagt ggaaacgacg ttgcgcggca tcgcgccgca tattgaaggg
    35581 aaaacggtgg aagccgtggt attgcgccaa ttgaagctgc gctggcagat caatcccgat
    35641 ttgggggaga ttttgtccgg tcggcaggtt ttgtcctgcg gcaggcgtgc caaatatctg
    35701 attgtccgtt tccaaacggg gattttgctg attcacttgg ggatgtcggg cagcttgcgg
    35761 atttttacgc cgtcggacgg acgtatcggc aggccggaca gacacgatca cgtcgatatt
    35821 gtgttttcag acggcacggt catgcgttac cgcgatccga gaaagttcgg cgcgatactt
    35881 tggtatgagg gaatcgaaga acatcatccg ctgttggaaa aactggggcc ggagcctttg
    35941 tcggaggcat tttgtgcgga ttatctgtat gcaaggctga aggcgcagaa gcgcgcggtc
    36001 aaacttgccc tgatggacaa tgcggtcgtg gtcggtgtgg gcaacattta tgccaacgag
    36061 agcctgttta gggcgggcat ttcgccccac cgtcctgcca accgcctgaa aaagaaagag
    36121 tgcgcgcttt tggttgaaac cgtcaaagcg gtgttgcagc gcgccattga aacgggcggc
    36181 agtacgctga gggactttgt ggacagcgac ggcaaaagcg gctacttcca acaggaatat
    36241 acggtgtacg ggcggcacaa tcagccgtgc ccccagtgcg gcggtttggt tgtgaaagaa
    36301 actttggggc agcgcggcac gttttactgc ccgaattgtc agaaataggg cggaaaacgg
    36361 tttcagacgg cattttatcg gtatgccgtc cgaacgtttc aacaacaaac accgattatc
    36421 gggaaagaat tgctcatgtc ttcaaataaa gcttcatttt ttacacgtct gcgccgcttg
    36481 tgccgcttga cggtctggct gttcaaaacc gggaaaaacc tgcgcggtat tgacggcggt
    36541 tgccccgagt cgcgcaatcg ggcggtaatc gagttgggca ggggggtttt ggcggctttg
    36601 gatatcggat tggaggtggg cagacccgca cccgaacatc cgaacggtgt cttggttgcc
    36661 gccaaccacg tgtcctggct ggatattttc gcgatgagcg cggtttatcc gagcagcttt
    36721 atcgccaagc aggaaatcaa aagctggccg gtattgggca agatggggca gaacgcggga
    36781 acggtgttca tcaaccgcaa ttcgcggcgc gacatcgaac cgattaaccg cgccgtctgc
    36841 gaaaccttgc aacgcggtca aaacgtcagt tttttccccg aagcgcggac ttcctccgga
    36901 ttggggcttt tgccgttcaa agccgcgctg ttccaatccg ccatcgatgc gggggcaaag
    36961 gttttggcgg tcgcgctgcg ttattatgac gaaacgggaa aaaggacggc tcgtccctca
    37021 tatgccgatg tcggtttgcc gacctgcctg tggcgcatcg tgtctatgaa aaaattgacg
    37081 ataaaagtcg atttcgtttg cgtggcggat gcggcggaaa gcgaagaccg ttatgcttta
    37141 aaagataaaa tcgaagaaag catccgtgcc gttgtcgccg acgatgcgga tatcgctgtc
    37201 tgaaaccggt tgtcggaatg tggcagtatg attcgctttt gtggatgtat agtggattaa
    37261 atttaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
    37321 gaagcaccaa gtgaatcggt ttcgtactat ctgtactgtc tgcggcttcg tcgccttgtc
    37381 ctgatttttg ttaatccact atattaaggc tgcggcaacg ggaggacctc aaacgaaatg
    37441 gcgtaaggat agtttgttgt attcataaaa tatgccgtct gaacctgtgt tcagacggca
    37501 tattttatat gggattaacg gcggaacctg ctgccgtcga aatcatcgaa agcctcataa
    37561 gccgcccggc attgggtttt ctgttcctta taaggcaggg agcggaatgc tttgcgcgcc
    37621 tttttgtttt cgcgcaattc ctgcacgttg ccttgcgatc ggtaccacgc ggcacggcgt
    37681 tccaaatatt tcctgcattc ggggtgcagc atctgcttgt ttgccttgcg gatatgtttg
    37741 gtatcgcggt gggcgggttc tgacgtaccg gcagcggcag ttgtcgatac cagtcccaat
    37801 attgcagaca acagtatggc aagcatcttt ttgacagata tgacgatcct ttcagtttgt
    37861 cattccttcc agtataggat ggaaggatgg aacgggcaaa ttgtcggcag tttcagactc
    37921 attcagccga caaaacttgc cgtttgatat gcggattcaa ataacacatt aatctcaata
    37981 ataaattcca atcggtaaga aaatggaatt tgtcggcggc ggcgcggcaa aatcatactt
    38041 tgcaaaattt aacaatttgc aggggcagaa aacaggaagc tttccttttt cgtcggaaaa
    38101 tccttatttc gccgccttgt agccggagcc ggtcaaaagg caaaaaattt acccgttttt
    38161 tatcggtaaa gaattatcag ataaaacaaa tattatagga aaaatacgac aggcgggttt
    38221 tatcgcgcat tgcctgaaac tgaaaaatac aaccgttgtc aagactggag aaaatgccaa
    38281 aaatccacta tattgtctgc cttaatttat ttgaaaagac tgtgtcttga atatcaagag
    38341 tggaagagga agcgatgaat acaccgactg atttgaaagt aaccaaacga gacggaagat
    38401 tagaagccat tgatttggat aagattcacc gtgtcgtcac ttgggcggcg gacggattgg
    38461 aaaatgtttc cgtatctcag gtcgagttga aatcgcacat ccagttctac aacggcatcc
    38521 gcaccgacga catccacgaa accatcatca aagccgctgc cgatttaatt tcggaagata
    38581 ccccggacta ccaatacctt gccgcgcgtt tggcgatttt ccatctgcgt aaaatagcct
    38641 acggcgagta cgagccgccg cacctttacg accacgttaa aaaacttacc gatgccgcaa
    38701 aatacgacag gcatatcctt gaggattaca gccgcgaaga atttgacgag ctgaacgcct
    38761 atatcgacca cgaacgcgat atgtcctttt cctatgccgc tgtcaaacag ctcgaaggca
    38821 aatatctggt acagaaccgc gttacccgcc agatttatga aacaccgcag ttcttatatg
    38881 ttctggtggc gatgtgtctt ttcagcaaat atcccaaaga aacacgcctc gattacgtca
    38941 aacggtttta cgatgccgtt tccacattta aagtatcgct gccgaccccg attatgagcg
    39001 gcgtgcgtac gcctacgcgc cagttctcaa gctgcgtatt gatcgaatgc gacgacagcc
    39061 tggattccat caacgccacc accagcgcga ttgtgaaata cgtttcccag cgtgcgggca
    39121 tcggcatcaa tgccggacgt atccgcggac tggacagcga aatccggggc ggcgaagcac
    39181 ggcataccgg ctgcattccc ttctttaaaa tgtttcaggc ggcagtcaaa tcctgttcgc
    39241 aaggtggcgt gcgcggcggc gcggcaacct tgttctaccc cttgtggcat atcgaagccg
    39301 aaagcctgct ggtgttgaaa aacaaccggg gcgtggaaga caaccgtatc cgtcagcttg
    39361 attacggcgt gcaaatcaac cgcctgctgt acacccgcct gattaagggc ggcaacatta
    39421 cgctgttttc gcccaacgag gttccgggat tgtacgaagc gttttttgcc gaccaagacg
    39481 aatttgagcg gctctatacg aaatacgagc aagaccctga tatccgcaag cgcatcattc
    39541 cggctgccga cctgttttcc acgctgatgc aggagcgtgc cggaaccggg cgcatctaca
    39601 ttcaaaacgt cgatcactgc aatacgcaca gcccgttcga tccgcgcgtt gcgcctgttc
    39661 atcagtccaa cttgtgtatg gaaatcgcgc tgccgaccaa acctttggac aatatcaacg
    39721 acccgaacgg cgaaatcgcc ctgtgtaccc tgtctgcctt caacttgggg gcattaaaca
    39781 gcttggacga attggaaggg cttgccgatt tgaccgtgcg cgcgctcgat gcacttttag
    39841 attatcaggg atatccggta gaagccgcgc gtacctccac tatgggccgc cgttcgctcg
    39901 gtatcggcgt gatcaactat gcgtattatt tggcgaaaaa cggtgtccgc tacagcgacg
    39961 gctccgcgct cggtctgacc caccgtacct ttgaagccat acagtattac ctgctcaaag
    40021 catcggcaaa ccttgccaaa gaatacggtg cgtgcacgct ctttaaccaa accgtttatt
    40081 cgcaaggcaa actgcccatc gacacctaca aaaaagactt ggacgctgtg tgtaacgagc
    40141 ctttgcacta cgattgggaa agcctgcgtg ccgacatcgt caaatacggc ctgcgtaact
    40201 ctactctaac cgcactcatg ccgtctgaaa ccagttcgca aatcgccaac gccaccaacg
    40261 gtatcgagcc gccgcgcgga ttggtaacgg tcaaagcatc gaaagacggc attttgaaac
    40321 aagtcgtgcc ggagtttgaa accctgaaaa atgcctatga aaccctgtgg cagcttcccg
    40381 gcaacgaagg ctatctgaaa cttgttggcg tgatgcaaaa attcgtcgat caggcgattt
    40441 ccgccaacac cgcctacgac ccgggcaaat tcgaaggcgg caaagtttct atgaaacaaa
    40501 tgctcaaaga cctgctgacc gcctacaaat acggcgtcaa aaccctgtac taccataaca
    40561 cccgcgacgg cgcggacgat acgcagaccg atattcagga tgacggctgc gcgggcgggg
    40621 cttgtaagat ttgatgaaag ggggagtttt cagatggcct ttagattaat aaatcatctg
    40681 aaatataaaa tatgaaaaat aaaatacaaa atcaattaga taatatagtt accttaaata
    40741 atgagaaatt tacttttatt gatttatttg cgggaatagg tggttttcgc attgcaatgg
    40801 agaatgttgg tgggcgatgt gtattttcta gtgaatggga tgataaagcc cgtcaaacct
    40861 accaagtaaa ttttaatgat attccttatg gagatattac attaaaggaa accaaagcag
    40921 ctattccaag taagtttgat gtattaacag caggattccc gtgtcagcca ttctctatag
    40981 ctggtgtttc aaagaaaaaa agcctaggac gagaaacagg cttcttagat aaggcgcagg
    41041 gaactctatt ttttgatgtt gctgaaatta ttggaaaaca tcgacctaaa atttttcttt
    41101 tagagaatgt gaaaaacctt gtttcgcatg acaaaggaaa tacatttaaa gtaattaaag
    41161 ggactttaga agagcttgac tatcagatat tttatcaagt tatgaatgca aaatattatg
    41221 ttcctcaaaa tagggagcgt atttttattg taggttttga tagacaatat tttaataagg
    41281 aaataaattt caattttcct tccccaccag aatcacaacc aaaattaaag caaattttgg
    41341 aagatgatgt agataattct tttactcttt ctgataactt atggctttac cttcaaaatt
    41401 acgctaaaaa acataaggca aagggtaatg gatttggttt tggattagtt gatttagatg
    41461 gaatatcacg aactctatct gcacgatatt acaaagatgg ttccgaaata ctcatccctc
    41521 agaaaggaaa aaatcctagg aagctgacac ctagggagtg ctcgcgttta atgggatttc
    41581 ctaaagattt tgttattgat gcagtatcca agacagctgc atacaagcag tttggcaatt
    41641 caattgctgt accgttggtt caagctattg ctaaacaaat tataaatgag ttaaaaaatg
    41701 aatgacttgg cagctcagac aatacaagta gtaaataaat ccgatgactt agtagcttca
    41761 gcaatacaga cagcaaataa atcaattgca gtatactgtc gttatattcg tcccaatgat
    41821 gttggtacta ctggtagtca ccaatcagga ttttatattc aaaaacattt ttcagacgac
    41881 ctctttgatg tagtttgcca aaagggaaca aataaaacta tttcaatcaa aattaattgg
    41941 caagatggaa gcgtcactaa tagcaatttt aaatattacg gccaaggcac gagaaatgag
    42001 gcgagaatta cgggttttgg caagaatttt gaatttttaa gtgataaata tagtggttcc
    42061 ttattggtat tgtgcagagc ttgttataaa gatttattat tccatgcttt tgttttatct
    42121 tcagatgaag atattgaaat atttatcgca gaaactaaca ttttaccagg aagtttatat
    42181 ttacctaaaa aacaagaagt agaggataac ctaacaaaat tattttcact atttcctaac
    42241 ttccccaaaa ctgaagaaat ggcagttttg gcaagggaaa atattgcatt aaataaaact
    42301 aacgttagta atgtgttaaa acaatgggtt tcaaaggaat atgaattatt ttccattttt
    42361 gaaaaacgtg aatttgaaat tattaaatca aaaattactg atttggatag cttcattaat
    42421 tttgcacatt cttttacaaa tcgcagaaaa gcaagagcag gaaaatcttt agagttacat
    42481 ttatcaagaa tatttgacga attttcctta aaatttgaga cgcaagcgaa gacagaaggg
    42541 aaaaagaaac cagatttttt atttcctgga agtgaagagt accatgcgat ggatgaatca
    42601 gggaatttca tatttcaaac tgaaaagctg acaatgctag gttcaaagac tacttgtaag
    42661 gatcgctggc gccaagtttt aaatgaagca gatcgaattc ctcataagca tttatttact
    42721 ctacaagaag gtatatctga tactcaaatt caggaaatga gtgatgagaa tttaacctta
    42781 gttgtcccta aagaatccgt aaaaacattt ggaacttttg ggaaaaccca tgttttaact
    42841 ttagaaaatt ttattaaata tataaagtct cagcaggtta gttaaaactt acagatcatt
    42901 tttaattaat atagagaata gattatgtcc tgcgaacact taactatgtc atacagcacc
    42961 tttcccaaaa ccaaaaacga cgcgctgaat gagccgatgt ttttcggtca gccggttaat
    43021 gtcgcccgtt atgaccagca gaaatacgag gtatttgaaa aactgattga aaaacaattg
    43081 tcgttttttt ggcggccgga agaaatcgac gtgtcgcgcg accgtatcga ctacgccaac
    43141 ctgccagaac acgaaaaaca tattttcatc agcaatctga aataccaaac actgctcgat
    43201 tccatccaag ggcgcagtcc gaatgttgcc ttgctgcctt tggtttcgat tcccgagttg
    43261 gaaacgtgga ttgaaacgtg gagcttcagc gaaaccatac actcgcgcag ctatacccac
    43321 atcatccgca atattgtgaa tgatccgtcg gtcgtgtttg atgatattgt cgaaaacgaa
    43381 tacattaccg cccgcgccga agacattgcc tgctattacg acgacttaat cgaatacacc
    43441 cagtattaca atctgttggg cgaaggggtg cacaatgtcg gcggaaaacc cattactgtg
    43501 tctttgcgcg ggttgaagaa aaaactctat ctctgcctga tgtgcgtcaa cgtgttggaa
    43561 gccatccgtt tctacgtttc attcgcctgc tcgtttgctt ttgccgagcg cgagttgatg
    43621 gaaggcaacg ccaaaatcat caaactgatt gcccgcgacg aagccctgca cctgaccggc
    43681 acgcagcata tgcttaatct gatgcgttcg ggtgtcgatg attctgaaat ggcagaaatt
    43741 gccgctgagt tgcaggacga atgtttccaa ctcttcaaaa aagcggcgga acaggaaaaa
    43801 gaatgggcgg catatttgtt taaagacggt tcgatgattg gtttgaacaa agaaatctta
    43861 tcccagtacg tcgaatatat taccaatctg cgtatgcagg cggtggggtt gccggccgga
    43921 tttgaaggcg caaatcaaaa cccgattccg tggattaatg cgtggctgtc gtccgataac
    43981 gtacaggtcg cgccgcagga agtggaaata tcctcttatt tgatcggtca gatcgattct
    44041 gaagtgaata cggatgattt gggcgatttt gagttgtaaa cgttttttga aaaaatgccg
    44101 tctgaagccg gatgtttcag acggcatttg tatgttgtat gatgggaaaa acagcgggta
    44161 ggatcggtta ttgttccttt tatagtggat tatagtggat taacaaaaat ccggacaagg
    44221 cgaggcaacg ccgtactggt ttaaatttaa tccactatat ttttgattgg tcggaaatgg
    44281 cgcgcattgg tacgaataag ggtttttttg agctcttgga gggcgagacc ctgttggaag
    44341 gcttggaacg gacggggcat atggtggaat atcagtgccg aagcggatat tgcggatcgt
    44401 gccgagtgaa gattttggag ggaagcgtta cttatcggga gccgcctttg gcttttttgg
    44461 ggcgggacga gatactgccg tgctgctgtt gcgtcgaggg ggatgtcagg ctggattgcg
    44521 gtttgtcggg ggaggatgag gggctgtctg acagtttgtt gaaataaaag ccgtccgaac
    44581 attggttcgg acggcttttt gacggtatta ccggttatgc ctgatgagtc ggtaaaaggt
    44641 ttcaccgtct tggatataac ccagttctac ccgggcgatt tccgaaatgg cttcttgacc
    44701 gttttccaaa tcatagactt cggcggcaag ggaatgattg cgtagggcga gtgtctggtt
    44761 tttttcttct tgaacggcaa tctgttctct cagactgctg ttgcgtccga tgctgccttt
    44821 gccgaaccag aggctgtatt ggcaacagac aagtgcgaag gataaaacga cagttaccca
    44881 cttcatacct taaccttttt tatttgccca gttggtagaa tgcggctttg ctggggtagt
    44941 cggcggcttc cgccaattct tcctcgatac gcagcagttg gttgtatttc gccatgcggt
    45001 cggaacggct caaagaaccg gttttgatct gcatacagtt ggtggcgact gccaagtcgg
    45061 caatggtgct gtcttcggtt tcgccggagc ggtggctcat tacgctggcg tagcggttgc
    45121 gtttggctaa gtcgacggct ttcagggttt cactcaaagt accgatttgg ttgactttga
    45181 ccaatagtgc gtttgccacg cctttttcaa tgccttcggc aaggattttc gggttggtaa
    45241 cgaagaggtc gtcgccaacg agttggactt tgccgcccag tttttcggtc agcagtttcc
    45301 agccttccca gtcgttttca tccatcccgt cttcgatgga gatgatgggg aactcgttga
    45361 ccaggccttc cagatattcg gcaaattccg cgttggtgta ggagcggcct tcggcttcca
    45421 agtggtattt gccgtctttg tagaactcgc tggacgcgca gtccaatgcg aataatacgt
    45481 cttcgcccgc tttgtagccg gcggcttcgg tcgcctcgac catcagttgc agggcttctt
    45541 tgtggctgtt caggttgggg gcgaaaccgc cttcgtcgcc gactgtggtc gggaagcctt
    45601 tgctgtcgca cagttttttc aaggcgtgga aaatttccgc accgcagcgc aacgcttcgc
    45661 ggaaagattt tgcgccgacg ggcataatca taaactcttg gatgttcagg ctgttgttgg
    45721 cgtgttcgcc gccgttgatg acgttcatca tcggtacggg cagggacatc gggcctgcgc
    45781 cgcccaagta gcggtaaagc ggcaggcctg agtcttcggc agcggcgcgt gcaaccgcca
    45841 tagaaaccgc caaagtcgca ttcgcaccca aattgccttt gttttcagta ccgtccaatt
    45901 cgatcatgat ttggtcgata taagattgct cgttggcatc gataccaatg agggcttggg
    45961 cgatttggtt gttgacgtgt tcgaccgcct tcaatacgcc cttgcccgaa taacgggatt
    46021 tgtcgccgtc gcgaagttcc aaagcctctt tttgaccggt ggacgcgccg ctcggtacgg
    46081 ctgcgcgtcc cattacgccg gattcgagca atacatcaca ctcgactgtg gggttgccgc
    46141 gtgagtccaa aatttcgcgg gcgaaaatat caacgattgc gctcatgaat gttctccaag
    46201 tgaagtgaag ggaggtaaaa aatgggaggg cttcagacgg catctttgcc ggaaagccgc
    46261 caacttaaaa ctgctgaaag tagggcggtt gccataaaaa caatactgga aatgccgtgc
    46321 cacattccga aggatccgcc tacaaacgac aacagccaat tttcatgtcc gtatttcagt
    46381 gcctcgataa cgggtgtaat caagaatcgg ttggcggcaa gggcggacaa taaaaaaagc
    46441 agcagggcgg tttgccgccg ggttagggcg gcaaaggcgg cagccagtac cgcgccccaa
    46501 acggcaagcc cgctccaaga gaggatgtcg aacaatacgc cggcaatttc gcccgcctgc
    46561 attttgggca gcattttgaa cagcaccggc gcgacgatat aacccgccat aatctgcata
    46621 ccgagccaca aggttgcggc ataccggctg atttttcgga aagtctgcat cataaggcga
    46681 aaccgtgtta ctcgattgtt aaaatcggtt gtgatttgat taaatcgtcc aatgctttga
    46741 tgcggattaa aaaatcttct aaaaggtgca gcggcagcgc gctggggccg tcgcattttg
    46801 ccagtttcgg atcggggtgc gattcgagga acagaccggc aaggcgggtt gccatgcctg
    46861 caagtgccaa atccaaagcc tgtgcgcgac gaccgccgga tgcggcagaa ccagcatcgc
    46921 gggtttgcag ggaatgggta acgtcgaaaa taaccggcag gttgccgcag gtttgtttca
    46981 tcacgcagaa accgagcata tcgacaacga ggttgtcgta gccgaagctg ctgccgcgtt
    47041 cgcataaaat cagtttcccg ttgccggctt cgtggaattt ttccacaatg tttttcattt
    47101 gagaggggct gaggaactga ggttttttga tgttgacgac gttgccagtt tttgccatgg
    47161 caaccactaa atcggtctgc cgcgcaagaa aggcgggaag ctggatgaca tcgcacactt
    47221 cggcgacggg ttggcactga tggggttcgt gtacgtcggt aatgacgggg atgccgaact
    47281 ctgctttgac tttttcaaaa atctttaagc cttcttccaa gcctacgccg cgataagaat
    47341 ggatggagga acggtttgcc ttgtcgaaag aggctttaaa gatataggga attccgagtt
    47401 tgcgggttac ttcgacgtaa tgcgcgcagg tttggagggt ggaatccaag ctttccaaaa
    47461 cgttgatgcc gccgaatagg acgaagggcg agttgttgcc gatggtgatg tcgttgattt
    47521 taatatccat aatcgatgtc cgtttgcgga attacagcac ggtgtgcggc ggctcgtagg
    47581 agatgatgtc gcggattttg ttgttcccgt ccactaagac gactttgggt tcatgcgcgg
    47641 cgatttcggg ttcggagagt tggacgtaag acatgatgat gacgatatcg cctttctgca
    47701 ccagccttgc cgcagcaccg ttcagacaaa tcacgccgct gccgcgttta cctgaaatgg
    47761 tataggtttc aaaacgttcg ccgttgttgt tgttgacgat ggcgactttt tcgttggggt
    47821 agatgcctgc cgcgtctaac agatcttgat cgacggtaat gctgccgacg tagttcaaat
    47881 cggcttcggt tacggtggcg cggtggattt ttccgccaag catagtacgg aacatgtatt
    47941 tccttttggt ttgtgtgcgg tctgacgggc gcgatgccgt ctggaaatct gaatgccgta
    48001 ttatagtgga gtttccttca aattcataat ggcggctttc ggacaaggaa aacggtgttt
    48061 cagacggcat tgcggtgtcg gctttatttg ggcaggcatc tgttttgcaa ggtttttaaa
    48121 acgtttttta cggtgttgat tctgacctcg atattttcca tctctgcggt aaaccgcagc
    48181 ttatcggcgc cggcaaggcg gtattttttg tcgttctgaa tcagcaggat gatttcggtt
    48241 ggatcgacat tgtggttttt gccgaaggtt accgttaccg attcgccggc cgcatcgatg
    48301 gcatcaatac ccaattcttt tgccgcaagc cgtaagtggt ggctttcgat gagggttttg
    48361 acgggttgtt cgggcaggcc gaagcggtcg acgagttctt cgtgtatggc gttgatttgc
    48421 tgcacggttt cgcagacggc gaggcgtttg tagaggacga gacgctcgtg gatgtcgggg
    48481 cagtagcttt cgggcagcag agcggggctg tgcagtttga tttcggtggt aatgcccaat
    48541 ggcgcgtcga ggtcgggctg gcggcctttt ttgaggtcgc gcacggcttg tttgagcatt
    48601 tcggtgtaga gcgtgaagcc gacctgtatc atttcgccgg actgcccttc gccgaggatt
    48661 tcgcctgcgc cgcggatttc caaatcctgc atggcgaggg tgaaacctgc gccgagttcg
    48721 tctgctgccg cgatggcgtc gaggcgtttt tctgcgtctt tggtgatgta ttcgggcgtg
    48781 agcaggtagg cgtaggcttg gtgatggctg cggccgacgc gcccgcgaag ttggtgcagt
    48841 tgcgccagcc cgaatttgtc ggcgcggttg atgatgatgg tgttggcgtt ggggatgtcg
    48901 atgccggttt cgatgatggt ggaacagagc aacacgttga atcgttgctg taaaaagtcg
    48961 cgcatgactt gttccaactc gcgctcgcgc agttgtccgt gcgctacgcc gatgcgggct
    49021 tcgggcagca gggtttccag ccgctcgcgc atattttcaa tcgtatctac ttcattgtgc
    49081 aggaaaaata cttgcccgcc gcgtttgagt tcgcgcaaca cggcttcgcg cacgctgcct
    49141 tcgctgaagg gtttgacaaa ggttttgacg gcgaggcggc ggctcggtgc agtggtaatc
    49201 agcgagaagt cgcgcagacc ttccaacgcc atacttaaag tacgcggaat cggcgtggcg
    49261 gtcatggtga ggatatcgac attggcgcgc aggcgtttga gctgctcttt ctgtcgcacg
    49321 ccgaagcggt gttcttcgtc gataatcact aaacctaagt ttttgaattt tatgtcgtcc
    49381 tgcaccaatt tgtgcgtgcc gataatgata tcgaccgtgc cgtccgccat gccttcgagc
    49441 gcggctttgg tggctttgct gttgttgaaa cgcgaaaggc cggcgacttt cacgggaaaa
    49501 tcggcgaaac ggtcggcaaa gttttgcgcg tgctgctcga ccagaagcgt ggttggggcg
    49561 agtacggcga cctgtttgcc acccatcacc gccacaaacg cggcgcgcag ggcgacttcg
    49621 gttttgccga aaccgacatc gccgcacaca aggcggtcca tcggcttcgc ctgcgtcaag
    49681 tctttaatca cggcggcaat ggcggctgcc tggtcttcgg tttcctcgta gccgaagccg
    49741 tcggcaaacg cctgataatc caactcgttg atttcaaact tgtgtcccga ttgggcggcg
    49801 cgttgggcat agagattgag caactcggcg gcggtatcgc gcgctttttc ggcagccttg
    49861 cgttttgcct tgttccacgc gccgctgccg agcttgtgca gggcaatgtt ttcatgcgcc
    49921 tgaccggagt agcggctgat taaatgcagt tgcgaaacag ggacataaag ctgcgcttcg
    49981 cctgcgtatt cgagcaacat catttcgttg gtttcgccgc ccaagtccat cgttaccaag
    50041 cccatatacc gcccgatgcc gtgttcttcg tgcacgacgg gatcgccgat gttgatttcg
    50101 gcaaggtcgc gcaacaggcc atctgaaacg gcggcgtgtt tcttgcgatg atatttgcgc
    50161 gagcgggcaa catattgata caaatcggat tcggtaatga cagcgatggc t
//
LOCUS       DAWFKN010000005        46427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFKN010000005 DAWFKN010000000
VERSION     DAWFKN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.005"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..251)
                     /locus_tag="WGR46_000276"
     CDS             complement(<1..251)
                     /locus_tag="WGR46_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HEZ0855295.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYEMYITDGKGNLLGVPLRRGVSDSAFIDQ"
     gene            354..569
                     /locus_tag="WGR46_000277"
                     /pseudo
     CDS             354..569
                     /locus_tag="WGR46_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(721..1779)
                     /gene="trpD"
                     /locus_tag="WGR46_000278"
     CDS             complement(721..1779)
                     /gene="trpD"
                     /locus_tag="WGR46_000278"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0855296.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1843..2433)
                     /locus_tag="WGR46_000279"
     CDS             complement(1843..2433)
                     /locus_tag="WGR46_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0855297.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2853..5129
                     /locus_tag="WGR46_000280"
     CDS             2853..5129
                     /locus_tag="WGR46_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0855298.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            5813..5920
                     /locus_tag="WGR46_000281"
                     /pseudo
     CDS             5813..5920
                     /locus_tag="WGR46_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6065..6862)
                     /locus_tag="WGR46_000282"
     CDS             complement(6065..6862)
                     /locus_tag="WGR46_000282"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0855299.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            7017..9866
                     /gene="uvrA"
                     /locus_tag="WGR46_000283"
     CDS             7017..9866
                     /gene="uvrA"
                     /locus_tag="WGR46_000283"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0855300.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEQSITGQYLSSKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            9900..11495
                     /locus_tag="WGR46_000284"
     CDS             9900..11495
                     /locus_tag="WGR46_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ0855301.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(12046..12936)
                     /gene="sucD"
                     /locus_tag="WGR46_000285"
     CDS             complement(12046..12936)
                     /gene="sucD"
                     /locus_tag="WGR46_000285"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0855302.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(12947..14113)
                     /gene="sucC"
                     /locus_tag="WGR46_000286"
     CDS             complement(12947..14113)
                     /gene="sucC"
                     /locus_tag="WGR46_000286"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0855303.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(14212..14499)
                     /locus_tag="WGR46_000287"
     CDS             complement(14212..14499)
                     /locus_tag="WGR46_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855304.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(14594..16027)
                     /gene="lpdA"
                     /locus_tag="WGR46_000288"
     CDS             complement(14594..16027)
                     /gene="lpdA"
                     /locus_tag="WGR46_000288"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0855305.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            16224..16340
                     /locus_tag="WGR46_000289"
                     /pseudo
     CDS             16224..16340
                     /locus_tag="WGR46_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16392..17573)
                     /gene="odhB"
                     /locus_tag="WGR46_000290"
     CDS             complement(16392..17573)
                     /gene="odhB"
                     /locus_tag="WGR46_000290"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0855306.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(17673..20501)
                     /locus_tag="WGR46_000291"
     CDS             complement(17673..20501)
                     /locus_tag="WGR46_000291"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0855307.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SVRAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADAYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQPIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(20706..21989)
                     /gene="gltA"
                     /locus_tag="WGR46_000292"
     CDS             complement(20706..21989)
                     /gene="gltA"
                     /locus_tag="WGR46_000292"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0855308.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(22101..22349)
                     /locus_tag="WGR46_000293"
     CDS             complement(22101..22349)
                     /locus_tag="WGR46_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0855309.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(22353..23060)
                     /locus_tag="WGR46_000294"
     CDS             complement(22353..23060)
                     /locus_tag="WGR46_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0855310.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(23181..24944)
                     /gene="sdhA"
                     /locus_tag="WGR46_000295"
     CDS             complement(23181..24944)
                     /gene="sdhA"
                     /locus_tag="WGR46_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0855311.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(24947..25288)
                     /gene="sdhD"
                     /locus_tag="WGR46_000296"
     CDS             complement(24947..25288)
                     /gene="sdhD"
                     /locus_tag="WGR46_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0855312.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(25282..25659)
                     /gene="sdhC"
                     /locus_tag="WGR46_000297"
     CDS             complement(25282..25659)
                     /gene="sdhC"
                     /locus_tag="WGR46_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0855313.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(25938..27341)
                     /locus_tag="WGR46_000298"
     CDS             complement(25938..27341)
                     /locus_tag="WGR46_000298"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0855314.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(27590..28327)
                     /locus_tag="WGR46_000299"
     CDS             complement(27590..28327)
                     /locus_tag="WGR46_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0855315.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(28606..30882)
                     /gene="metE"
                     /locus_tag="WGR46_000300"
     CDS             complement(28606..30882)
                     /gene="metE"
                     /locus_tag="WGR46_000300"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0855316.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(31020..31898)
                     /gene="metF"
                     /locus_tag="WGR46_000301"
     CDS             complement(31020..31898)
                     /gene="metF"
                     /locus_tag="WGR46_000301"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0855317.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            32099..32374
                     /locus_tag="WGR46_000302"
     CDS             32099..32374
                     /locus_tag="WGR46_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0855318.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            32374..32499
                     /gene="ykgO"
                     /locus_tag="WGR46_000303"
     CDS             32374..32499
                     /gene="ykgO"
                     /locus_tag="WGR46_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0855319.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(32607..32807)
                     /locus_tag="WGR46_000304"
     CDS             complement(32607..32807)
                     /locus_tag="WGR46_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855320.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            32957..34096
                     /locus_tag="WGR46_000305"
     CDS             32957..34096
                     /locus_tag="WGR46_000305"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0855321.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            34093..34674
                     /gene="metW"
                     /locus_tag="WGR46_000306"
     CDS             34093..34674
                     /gene="metW"
                     /locus_tag="WGR46_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0855322.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            34791..35636
                     /locus_tag="WGR46_000307"
     CDS             34791..35636
                     /locus_tag="WGR46_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0855323.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(35686..36246)
                     /gene="efp"
                     /locus_tag="WGR46_000308"
     CDS             complement(35686..36246)
                     /gene="efp"
                     /locus_tag="WGR46_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0855324.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(36289..37437)
                     /gene="earP"
                     /locus_tag="WGR46_000309"
     CDS             complement(36289..37437)
                     /gene="earP"
                     /locus_tag="WGR46_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0855325.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(37456..37734)
                     /locus_tag="WGR46_000310"
     CDS             complement(37456..37734)
                     /locus_tag="WGR46_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855326.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            38236..39177
                     /gene="miaA"
                     /locus_tag="WGR46_000311"
     CDS             38236..39177
                     /gene="miaA"
                     /locus_tag="WGR46_000311"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0855327.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            39181..39900
                     /locus_tag="WGR46_000312"
     CDS             39181..39900
                     /locus_tag="WGR46_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0855328.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALSQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            39905..40393
                     /locus_tag="WGR46_000313"
     CDS             39905..40393
                     /locus_tag="WGR46_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0855329.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            40460..41212
                     /gene="rlmB"
                     /locus_tag="WGR46_000314"
     CDS             40460..41212
                     /gene="rlmB"
                     /locus_tag="WGR46_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0855330.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(41274..42665)
                     /locus_tag="WGR46_000315"
     CDS             complement(41274..42665)
                     /locus_tag="WGR46_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0855331.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            43125..44000
                     /locus_tag="WGR46_000316"
     CDS             43125..44000
                     /locus_tag="WGR46_000316"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0855332.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALVRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            44009..45205
                     /gene="bamC"
                     /locus_tag="WGR46_000317"
     CDS             44009..45205
                     /gene="bamC"
                     /locus_tag="WGR46_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0855333.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(45262..46194)
                     /gene="pip"
                     /locus_tag="WGR46_000318"
     CDS             complement(45262..46194)
                     /gene="pip"
                     /locus_tag="WGR46_000318"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0855334.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT        11771 a        12519 c        11175 g        10962 t
ORIGIN      
        1 atttggtcga tgaaagccga atcggataca ccgcgcctaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta catttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtacccct cctgttagat aaggggggaa gatttgaagc ggttgccggc
      181 ttcctgccgt ccgctaacgc gtccgtcatc acgccgacaa ccgcctttgt catcccttgc
      241 ttatcttcca tggtgcgaat cctcaaaaac aggcaaaaaa aaagccctgt tacttgtaga
      301 aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata tataaggttt
      361 ataccgtcgt tgttcctaat gcgcaatcag cgacattgtt gccaattatc cgaaagaaag
      421 ttaagcctga tggcattgta tacacggata cctttcgtag ttatgatgtg cttgatatta
      481 gtgaatttag ccatttacgt aagtttaacg gcattcccaa agagcatttg gggctgcatt
      541 taaagaaatg ccaatggcat tttaaataga atctcaaatt cagattttaa gacaattagt
      601 taatgggaat ttggtctggt tatctagtac agccccaagt ttttatataa aaacagtttg
      661 gtaagttctc ttcgtgaagt gcttatgttt tgccacaaaa gttccataat catggcggca
      721 tcacccaagc tccatctttc caagaaaatg gcatctttgt tgtggttgaa aaccgacaaa
      781 ctcctctttt ttcgatttgg ccctgcctga atcgatggct tcccgtgcgg cagatatgcc
      841 gtctgaaagc gaagcagcga catttccggc atatagggcg gcggcggtat taagcaatac
      901 gatatcgcgc gcagcccctt ctttcccatc cagcacctca ttcattttca acaaagattc
      961 ctgagtattg gcaactttga tttcatccaa attgcggcgg gtttcgatac cgaaatcttc
     1021 tgggcggatg tcgtattcgc tgatttttcc gtctttgagt tcggcaacgc gcgttttgcc
     1081 cgtcagtgta atttcatcca aaccgccctc cccgcaaacg actaaaacgt gttttgaacc
     1141 gagttgttgc aagacccgcg acaaaatgcc gcacaaatcg gtgtggaaca cgcccaaaag
     1201 ctggttcggc gcgcccgcag gattggtcaa cggacccaat atgttgaaaa tgcttcggaa
     1261 accgagcgaa cggcgtaccg gggcgacatg gcgcatggca ctgtggtgat tgggggcgaa
     1321 cataaaccct ataccggtct ggctgatact ttgggcaatc tgttcgggag tcaggttgag
     1381 gtttgcgccc atctgctcca tcacgtccgc cgcgccgctg gaagatgaaa cggaacgccc
     1441 gccgtgtttg gcaaccttcg cgcctgccgc tgcggcaaca aacatcgaag tcgtcgaaat
     1501 attgaaggtt ttggcaccat ccccgcccgt accgacgata tcaaccagcc cctccgcatt
     1561 ctccagcggc acttttgtcg caaactcgcg catgacggct gcagctgcgg taatttcgga
     1621 aacggtttca accttgatac gcaatcctgt caaaatcgcc gcaatttgtt cgggcggcac
     1681 ttttccgctc atcatttggc gcatcaagtc ggtcatttca tcgtaaaaca actcattatt
     1741 gctgattaat cgttcgatag cctgttgcgg tgtaatcatt ttttgtcctc cgttcaatat
     1801 tcggacgaaa atgccgtctg aagggcttca gacggcatca cgtcagattt tttgcggttt
     1861 gaagttttga aattcgatta aaaaattgtt taacatatca tgtccgtgct cggtcaagag
     1921 ggcttcgggg tggaactgca cgccctcgac ggcatattcc ttatggcgca cacccataat
     1981 ctcgccgtcc tcagtccaag ccgttacttc caaacattcg ggcatcgtat tccgatcgat
     2041 aacgaggctg tgataacgcg tacaggtaac cggattgggc aaacccttaa acataccctt
     2101 gcccgaatgg gacacgggcg acaccttacc gtgcatcagc gttttggcgc ggactatcct
     2161 gccgccgaac gcctcgccta tcgtctgatg cccgaggcac acgcccataa tcggcagccg
     2221 gccggcaaaa tggcgcatcg ccgccacgga aatccccgct tctttgggcg aacacgggcc
     2281 gggtccgata acgagatatt gcggattcaa tgcctcgatt tcttccaacg taatatcatc
     2341 gttgcggcgc acggcgactt cctgccccaa ttcagcgaaa tactggacga tgttgtaagt
     2401 aaaactgtcg taattgtcga taaacaaaag cattttgtga ttaacctatt gatttataat
     2461 ttgtttgtta ttaactgcat ttccgatacc accatccata ccccttatat cttagcgtgc
     2521 ccgatgcgcc ctcgtgaacc tgggcagagc ctcgcgttct gacaagtacg ccattttgcc
     2581 accttttcga ccgttcggga acgtgccgtt tttatgcact cataaatttt ggttatcccg
     2641 cagccgaaca tagtgggcaa acacagcgga atctgcgtca acatcaaccg cgcccccgaa
     2701 tgacggcgga tacaaatgga caccgcccga tgccgcaacc gtttcatttg aaaaactttt
     2761 gcctatgccc tctgagtgtt ctcaaaatct tatgatataa aaaatgtaat gttatataat
     2821 aacaaacttt tgaaatgcga cgagaagcca aaatggcaca aactacactc aaacccattg
     2881 ttttatcaat tcttttaatc agcaccccca tcctcgccca agcgcatgaa accgagcagt
     2941 ctgttgactt ggacgaggtt accgtcgtcg gcaaaagccg tccgcgcgcc acatccgggc
     3001 tgttgcacac ttcgaccgct tccgacaaaa tcctctccgg cgataccttg cgccaaaaag
     3061 ccgtcaactt gggcgatgct ttggacggcg tgccgggcat tcacgcctcg caatacggcg
     3121 gcggcgcgtc cgctcccgtt attcgcggtc aaacaggcag gcggattaaa gtgttgaacc
     3181 atcacggcga aacgggcgat atggcggatt tttcgcccga tcacgccatt atggtagata
     3241 ccgccttgtc gcaacaggtc gaaatcctgc gcgggccggt tacgctcttg tacagctcgg
     3301 gcaatgtggc ggggctggtc gatgttgccg atggcaaaat ccccgaaaaa atgcctgaaa
     3361 acggcgtatc gggcgaactc ggattgcgtt tgagcagcgg caatctggaa aaactcacgt
     3421 ccggcggcat caatatcggt ttgggcaaaa actttgtatt gcacacggaa gggctgtacc
     3481 gcaaatcggg ggattacgcc gtaccgcgtt accgcaatct gaaacgcctg cccgacagcc
     3541 acgccgattc gcaaacgggc agcatcgggc tgtcttgggt tggcgaaaaa ggttttatcg
     3601 gcgtagcgta cagcgaccgt cgcgaccaat atggtctgcc tgcccacagc cacgaatacg
     3661 atgattgcca cgccgacatc atctggcaaa agagcttgat taacaaacgc tatttacagc
     3721 tttatccgca cctgttgacc gaagaagaca tcgattacga caatccgggc ttgagctgcg
     3781 gcttccacga cgacgatgat gcacacgcac acacccacag cggcagaccg tggatagacc
     3841 tgcgcaacaa acgctacgaa ctccgtgccg aatggaagca accgttcccc ggttttgaag
     3901 ccctgcgcgt acacctgaac cgcaacgact accgccacga cgaaaaagca ggcgatgcag
     3961 tcgaaaactt ttttaacaac caaacgcaaa acgcccgcat cgagttgcgc caccaaccca
     4021 taggtcgtct gaaaggcagc tggggcgtgc aatatttaca acaaaaatcc agtgctttat
     4081 ctgccatatc cgaagcggtt aaacaaccga tgctgcttga caacaaagtg caacattaca
     4141 gctttttcgg tgtagaacag gcaaactggg acaacttcac gcttgaaggc ggcgtacgcg
     4201 tggaaaaaca aaaagcctcc attcagtacg acaaagcatt gattgatcgg gaaaactact
     4261 acaaccaccc cctgcccgac ctcggcgcgc accgccaaac cgcccgctca ttcgcacttt
     4321 cgggcaactg gtatttcacg ccacaacaca aactcagcct gaccgcctcc catcaggaac
     4381 gcctgccgtc aacgcaagag ctgtacgcac acggcaaaca cgtcgccacc aacacctttg
     4441 aagtcggcaa caaacacctc aacaaagagc gttccaacaa tatcgaactc gcgctgggct
     4501 acgaaggcga ccgctggcaa tacaatctgg cactctaccg caaccgcttc ggtaactaca
     4561 tttacgccca aaccttaaac gacggacgcg gccccaaatc catcgaagac gacagcgaaa
     4621 tgaagctcgt gcgctacaac caatccggcg ccgacttcta cggcgcggaa ggcgaaatct
     4681 acttcaaacc gacaccgcgc taccgcatcg gcgtttccgg cgactatgta cgaggccgtc
     4741 tgaaaaacct gccttcccta cccggcagag aagatgccta cggcaaccgt cctttcatcg
     4801 cacaggacga ccaaaatgcc ccccgtgttc cggctgcgcg cctcggcttc cacctgaaag
     4861 cctcgctgac cgaccgtatc gatgccaatt tggactacta ccgcgtgttc gcccaaaaca
     4921 aactcgcccg ctacgaaacg cgcacgcccg gacaccatat gctcaacctc ggcgcaaact
     4981 accgccgcaa tacgcgctat ggcgagtgga attggtacgt caaagccgac aacctgctca
     5041 accaatccgt ttacgcccac agcagctttc tctctgatac gccgcaaatg ggccgcagct
     5101 ttaccggcgg cgtgaacgtg aagttttaaa atcggacagg caaacaaaaa aggttttcag
     5161 acggcattct gaaaaccttt tttcttttta atcaatttgt taggaaatat ggcaaagaca
     5221 aagaacaaac cactatgcca tcattcccat tttttttgtt tcatcattct tactttcacg
     5281 ccgtcattct cgcaaaagca gaaaaatcaa aagcagaaac ccaagatccc gtcattcccg
     5341 cgcaggcggg aatccaattc gttcggtttc agccgtttcc gataaatcac tgtaacgtta
     5401 agttcccgga ttcccacttt cgtgggaatg acggcgtaaa ggtttctgtt ttttaagttt
     5461 taggtaacta ataagtcgtc attcccgcgc aggcgggaat ccagtctctt cgttttcagc
     5521 cgtttccgat aaatcactgt aacgttaagt tcccggattc ccactttcgt gggaatgacg
     5581 cgtaaaggtt tctgtttttt aagttttaag taactaatga gttgtcattc ccacgcaggc
     5641 gggaatccgg tctcttcggt ttcagtcgtt tccgataaat cactgtaacg ttaagttccc
     5701 ggattcccac tttcgtggga atgacgcgga aaggtttctg ttttttaagt gttaggtaac
     5761 taatgagttg tcattcccgc gcaggcggga atctaattcg ttcagtttct gttatagtgg
     5821 attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
     5881 cgaggcaacg ccgtactggt ttaaatttaa tccactatat ttctgataaa tcaccgtaac
     5941 gttaaatttc cagattccca ttttcgtgaa cataacggtg tgaacattag caaaaattca
     6001 aaaaaccaaa cttcctcatt cgtgtgaatg atggtactga tattttctat ttcagttcgc
     6061 aaaattaatc tttcaacaca tcttgcacag ccgcctgaat cttagccgcc gctgcctcga
     6121 tttgccgttg ttcggcagat gggtttgccg ctgtttcaac cggagcagtt tgtaaagcag
     6181 gctcagactc aggctcggat tcgatttgcg gcgcagtcag cggcaaacgc gccaatacag
     6241 tgctaacgcc agttacttta tcgccaatcg ccacttgcgc ctgcgcatcg acaggcagat
     6301 acatatccac gcgcgaaccg aagcggataa agccataacg ttcgccgcgg gacagtttcg
     6361 cacctgcttg ggtgtagcac aaaatacggc gcgccaccaa accggccact tgaacaaaag
     6421 taatttcacg accggaagcc gtagtcgcca acaccgcatt acgttcgttt tccgtgctgg
     6481 ctttgtccaa atccgcattc acgaatttgc ctttgttata gaccactttc gttaccgtac
     6541 aatcggcagg cgatttttgc gaatgcacgt tgaacacgtt cataaaaata ctgattttca
     6601 aagcatcgac atcacgatac ggatcgcgtg cgcgctcgac caccacgata cggccgtcaa
     6661 ccggactcaa cactgcttca ggattttgcg gaatctcacg cgcagggtcg cggaaaaact
     6721 gcagggcaaa tacggtaaac acccaaaacg gcaaagacca ccagccgcaa cacatcgaca
     6781 ccagcaggct caaagccaaa ccgccgccaa taatcggcca gccctcacgg gcgataatcg
     6841 ggtgggggta aagacggttc atttgtcatc cttttgttca agcgggttaa aattgccgcc
     6901 attataacgg aatccgccgc gttccgccga caaatgccgt ctgaacgcgg cttcagacgg
     6961 catccgagaa agtgctacaa taccagtttt cctttatttt tcaggcggca tccattatgt
     7021 acaagcatca tccccaacat tcgcacgaca acgacaccat ccgcatccgc ggcgcgcgca
     7081 cgcataattt gaaaaacgtc gatttggaca ttccgcgcca caagctcgta gtggtaacag
     7141 gcttatcggg cagcggcaaa tcgtcgctgg catttgatac tttatatgcc gaaggtcaac
     7201 gccgttatgt cgagagcctt tccgcctatg cgcggcagtt tttgcagatg atggacaaac
     7261 ccgatgtcga tttgattgaa ggtctgtcgc ctgcgatttc tatcgagcaa aaatccacca
     7321 gccacaatcc gcgttccacc gtcggcacgg ttacggaaat ccacgactac ctgcgccttt
     7381 tgtacgcccg cgtcggcacg ccgtattgcc ccgaacacaa tctgccgttg tcgagccaaa
     7441 ccgtatcgca gatggtcgat gccgtgttga agctgccgga agacacgcgc gtgatgattc
     7501 tggcgccggc ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg
     7561 cgcagggttt tgcgcgcgtg tgcgtggacg gcgaggtcta tcagcttgac gaagtgccga
     7621 agctggaaaa aaacatcaag cacaacatcg acgtggtcat cgaccgcgtg aaagtgaagg
     7681 cggacatcaa gcagcggctg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc
     7741 gcgcgctggc gatggagatg gacagcggcg aagaacattg gttttccgcg cgtttcgcct
     7801 gccccgtatg ttcgtacagc ctgcccgaat tggagccgcg cctcttttcg ttcaacaacc
     7861 cgatgggctc ctgcccgact tgcgacggct tgggtaacac caatttcttc gactccgaaa
     7921 aagtggtcgc gcatcccgaa ttgtcgctgg cttcgggcgc gattgacggc tgggacaagc
     7981 gcaaccagtt ctatttccaa atgattcagt cgctggcacg gcattacggt ttcgacgtgc
     8041 aggctgcttg ggaaacccta cctgcaaaaa tcaaaaaagt cgtgttgcac ggctcgggca
     8101 aagaagtcat tgatttcact tacctgtccg aacgcggcac caccttcaac cgcagccacg
     8161 ccttcgaagg catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg
     8221 tgcgcgaaaa actgcgcgaa taccaaaacc accgcgcttg cccgagctgc ggcggcgcac
     8281 gtttgcgcaa agaagcccgc tacgtttacg tcggcggcga gccgttgcac gaagtttccg
     8341 cctggccgct gaccaaaacc caccgattct ttgaaacgct ggatttggac ggcaacaaaa
     8401 aacaaatcgc cgaaaaaatc ctcaaagaaa tcaccgaacg tctcggcttt ttgattaatg
     8461 tcgggctgga ttacctgaat ctctcccgtt ccgccgaaac cctttccggc ggcgaagccc
     8521 agcgcatccg cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg
     8581 acgaaccctc catcggcctg caccagcgcg acaacgaccg cctgcttgcc accctcaaac
     8641 gcctgcgcga tttgggcaac agcgtcatcg tggtcgaaca cgacgaagac gccatccgcg
     8701 aagccgattt cgtcgtcgat atgggcccgg gcgcaggcga acacggcggc aacgtactga
     8761 ttgccgacac gcccgaaaac gtcgccaaat gcgagcaatc cattaccgga caatacctca
     8821 gcagtaaaaa atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc gaccggatgc
     8881 tcgtcctcaa aggtgcgcgc ggcaacaacc tcaaaaacgt taccctcgaa ctgccgctcg
     8941 gtttgattac ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca
     9001 ccctcgccaa aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg
     9061 acgacatccg cggtctcgaa catctcgaca aagtcatcaa cgtcgaccaa tcccccatcg
     9121 gccgcacccc gcgctccaac cccgccacct acaccggcct gttcaccccc atccgcgaac
     9181 tcttcgccgg catccccctc tcgcgcgaac gcggctacaa cgtcggccgc ttttccttca
     9241 acgtcaaagg cggccgctgc gaagcctgcc aaggcgacgg cgtgattaaa gtcgaaatgc
     9301 acttcctgcc cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg
     9361 aaacgctcga aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgaccgtcg
     9421 aagaagcccg cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga
     9481 tggacgtagg tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg
     9541 aagcccagcg cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacactct
     9601 acatcctcga cgaacccacc accggcctac acttcgccga catcgccctg ctgctggaag
     9661 tcataggtcg tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctagacg
     9721 tgattaaaac cgccgattgg attgtggact tagggccgga aggcggcgat ggcgggggca
     9781 ggattattgc ttgtggtagc cctgagcagg tggcgaaagt taaggggagt tatactggga
     9841 agtatttgaa ggtggttttg aggtaagttt aattcaaaat taaataggag attattaaga
     9901 tgaaaccgtt aaaaacatta gaatttggat ttgtggatgc tgcaaactac agaagaagag
     9961 aaaataaaga tttatttaac cgaatatttg taaaaggaga atatttggat gaattatgtg
    10021 aaccaaatat ttcgttttta atcggagaaa agggaactgg aaagacagca tatgctgttt
    10081 atttaactaa taacttctat aaaaacatac atgccactac taagtttgtt cgtgaaaccg
    10141 attattcaaa atttattcag ctaaagaaag caagacactt aactgtttca gattttacaa
    10201 gtatttggaa agtcatttta tatctgttga tatcaaatca aatcaaatgt aaagaaaacg
    10261 gaatattatc ttcaatattt aataaattta aagccttaga tgaggctata aatgaatatt
    10321 attatggcgc ttttgatccg gaaattgtac aagcaataac tttaatagaa aattcaaaag
    10381 aagctgcgga aatgattttt ggaaaatttg ttaaactagg tgaagaggaa tcccaacaaa
    10441 taacttttac agaaagtaaa ttccaagcaa atttaggttt tattgaaaga aaatttaaag
    10501 atgctttatc tcagttaaag ctaaaagata atcatatttt gtttattgat gggatagata
    10561 ttagaccatc acagattcca tttgatgaat atcatgagtg tgtaaaaggt cttgctaacg
    10621 ccatatggat gttaaataat gatatcttcc cttccattaa agatagtaag ggaaggatga
    10681 gagttgtgtt attgattaga cctgatatct ttgattcatt aggtttacaa aatcaaaata
    10741 ccaaacttca agataattca gtatttttag actggaggac ggattataaa tcttatagaa
    10801 gttcaaagat ttttggcgtt tttgatcatc ttttgagaac ccagcaagaa aaacaagata
    10861 gtttagaaaa aggcaactca tgggattatt attttccatg gaatgctcct aatttacatg
    10921 atgagtataa aaatttaact tcatttatta gcttcctaag aaaatcgtat tatcgacctc
    10981 gcgatattct tcagatgctt actttgctac aaaaaaataa gaaaagtaag gaagattatg
    11041 tcgtagcaga agattttgat aatacttctt ttcaaagaga atactcgata tatttacttg
    11101 gtgaaatcaa agatcatctt ttgttttatt atagtcaaag tgattatcaa aatttcctga
    11161 aattttttga atttttaaac gggaaagata gatttaaata tagtgatttt ttaaaagcat
    11221 ttgaacgttt gaaaaagcac ttacaaacaa catcagtgga aatacctaaa tttatgagta
    11281 ctgctaatga gtttttgcaa tttttatttg acttgaatgt tattgcttat ttagataacc
    11341 cagaagatga aacgaaacca tatatccatt ggtgctttaa agatagaaat tatgcaaata
    11401 tttctcctaa aataaaaact gaaactgaat atttaatatt ttcaggatta tcaaaagccc
    11461 ttgatgttgg tactccattt aagaacaaac agtaacttgt atcccaacca accgtgtgcg
    11521 ctacgcacac ggttggtagc tgacgtgttg cagtttcaac agagaaagcc gtagcctgca
    11581 tacccaaccg accgcgtgcg tggctgtgcc acacgcccta cctgtgaatt acacggaact
    11641 tcaaagccaa tacgagagct ttgcaaaatt cctttccctc ccgacagccg aaacccaaac
    11701 acaggttttc ggctgttttc gtttcaaata tccactaatt ttaccccaat acccccttaa
    11761 tcctcccagg atacccgata atcaggcatc cgggccgcct tttaggcgca aacaggcaca
    11821 cttagcccga gacctttgca aaattcccca aaatccccta aatgtcttgg tgggaattta
    11881 ggggattttg gggaattttg caaaggtctc tgactgcccc aaaagggaaa aaataatgtg
    11941 ggtatgttga ggctgcctct ttttgaaatg acaaacaaaa tgccgtctga aacccgattt
    12001 ccggtttcag acggcatttg atgtttaatc agttgtcaaa cctgattatg ccaaaccttt
    12061 tgccttcaac acttccagca tcgtcgtgcc caactcggca gggctgcgag tataggcgat
    12121 accggctttt tcgaaagcgg cgaatttttc ttcggcagta cctttaccgc cggaaataat
    12181 cgcaccggcg tggcccatac gtttgccttt aggagcggta acaccagcaa tgtaacctac
    12241 aacaggtttg gttacgttgg attggatgta ttcggctgct tcttcttccg cagtaccgcc
    12301 gatttcacca atcatgatga tggcgtcggt atctgggtct tcttggaaga gtttcagtgc
    12361 gtcgatttgg ttcataccag gaatcgggtc gccgccgata ccgatacagg ttgattgacc
    12421 caagcccagt ttggtggttt gtgccacggc ttcgtaagtc aatgtaccgg aacgggaaat
    12481 aataccgatg cggcccggag tgtggatgtg gcccggcata atgccgattt tgcactcgcc
    12541 cggagtaatc acgcccgggc agttagggcc gaccaagcgg gtaccgttgc cgttggtttc
    12601 caagtaacgt ttggctttga gcatgtccaa agtaggtacg ccttcggtaa tcacaacgac
    12661 caagcctacg cctgaatcaa cggcttcaac gatagaatcc aacacaaacg gagcgggaac
    12721 gtaaattacg gatgcatccg cgccggtttc tttaacagct tctttcatgg tgttgaatac
    12781 aggcaggttc aggtgggttt gaccgccttt gcccggggta acgccgccga caactttagt
    12841 gccgtaagcc agagcttgtt cggagtggaa agtaccgttt ttaccggtga aaccttgaac
    12901 caatactttg gtgtctttat taatcaatac gctcattctt ttctccttag gcgtttacgg
    12961 ctgcaacaat tttttcggct gcgtcattca ggccgtctgc agaagtcagt ttcaggcctg
    13021 attcgttcag gattttcgcg ccgagttcgg cgttgttacc ttccaaacga acaacgacag
    13081 gaacgtcgac gttgatttct ttaacggctg ccacgatggc ttccgcaatc atgtcgcaac
    13141 gtacgatacc gccgaagatg ttgatcaata cgcctttaac ggatttgtct tccaaaatca
    13201 gtttgaacgc ttcaaccacg cggtctttgg ttgcgccgcc gccaacgtcc aagaagttgg
    13261 caggttgacc gcctttgagt ttgatgatgt ccatggtggc catcgccaaa ccggcaccgt
    13321 taaccataca gccgatgttg ccttccaggg caacatagtt caggtcgaat tcagaagctt
    13381 tcagttcacg ttcgttttct tgagatttgt cgcgcaattc agcgattttt ggcaggcggt
    13441 agagcgcgtt gctgtcgatg ccgattttgc cgtccacgca agccagtgcg ccgttttcgc
    13501 gaactgccag cgggttgact tcaaacaggg cgaagtcgtt ttcgacaaac gctttgtacg
    13561 cgccggtcat cagtttgacg aactcgttga tttgtttgtc tttcaagccc agttggaagg
    13621 caacttcgcg agcttggcaa ggttgcaggc cgaccagcgg atcaacggtt actttgaaga
    13681 ttttttctgg agtttcggca gcaacttttt cgatttctac gccaccttcg gtagaagcca
    13741 tgaatgtaac gcggcgggta gaacggtcaa ccactgcgcc caagtacagc tcggtttgaa
    13801 ccggatacat gtcttcgcaa accaaaacgc tgttgacagg ttggccgttg gcatcagttt
    13861 ggtaagttac caagttggtg ccaatcaggc tttcagccac ttctttagct tcttcgcggc
    13921 ttttaacgac ttttacgccg cccgctttac cgcggccgcc ggcgtgtact tgtgctttga
    13981 caacagcgaa tttgccgccc aatttgtcgt aagctgcagc ggcttcttcg ccgttgtgtg
    14041 ccaaaatacc gccttgtacg ggcaaaccgt agctagccag cagttcttta gcctgatact
    14101 cgtgtaaatt catggatttc tcctttagat tagtggaatg ccccgagttt gcctccaaat
    14161 ggcaagcccg tgcgtttcag actacctttc ggcggtcgga aaatcgtcgt ttcacttgct
    14221 gaaaggcaac cacatccgcg ccagcagata catggctgca ccgcctcccc cgagaataaa
    14281 aaagacgatg ccttttttac gcgaattcgg acagagcaga taaacggcaa ggctgaaact
    14341 gataaacaac aatgccacgc gccaaaccaa ttctttatct ttgaaacggt tgagcgtata
    14401 agtttcggtc atcatcacat acatctgcca caatgcggcc atacacatgc tgatgatgcc
    14461 cgccggtata aagaaaatac cgactatttc tttgttcata atgaatgtaa atgctgtttg
    14521 tttgtctgac ggaattataa atcagaaaac gaagctctcg gaacattcaa tttgtcggag
    14581 gccttaatgt ctatcaaccg tgcaaagcgc gtttgtcggc cgccaatgca gcttcgtgaa
    14641 ccacttcgga caaggttggg tgggcatgga taatgcgggc gatgtcttcg ctgctggcga
    14701 agaattcgag cgcagtcacg ccttcggtaa ccaattcgct gacaaccgga ccaatcatgt
    14761 gtacgcccaa gatgcggtcg gttttggcgt ctgccaacac tttaaccgta cctttggctt
    14821 tgcccattgc caatgcgcga ccattcgcac caaaacctga agtacctttt ttgtactcca
    14881 cgccttcggc tttaagctgt tcttcggttt tacccaccca agcgatttca ggatcggtgt
    14941 aaataacaaa cggtacgttg ttgaaatcga tatgcggttt ttgaccggca atgcgctcgg
    15001 caacggcaac gccttcgtcg ctggctttgt gtgccaacat cgggccgcga accacgtcgc
    15061 cgattgccca tacgttaggc aggttggtac ggcattcgcc atctactttg ataaagccgc
    15121 gctcgtcttt ttccaagcct acggcttccg cgttcaggcc tttggtgttt ggaatacggc
    15181 cgatggcaac gatcagtttg tcgaatactt cggttttggc ttcgccggca gcagtttcgt
    15241 aagcaacgga aacacctttg ccttcagact tgatgtcgcc gattttcacg cccaattcga
    15301 tgctcagacc ttgctctttg gtgaagtatt tgaaggcttc tttggcgatt tgttggtcgg
    15361 cggcagccag gaaggtcggc gcggcttcaa gaatggtaac ttccgcaccc acgcggttcc
    15421 atacggaacc catttccaaa ccaatcacac cggaaccgat cacgccgagt ttggcaggta
    15481 cttcggtcag gttcaatgcg ccttcgttgt ccaatacgtt cacattgtcg atagcgactt
    15541 gtggcagcgg acgcggtacg gaaccggtcg ctacgatgac gtgtttggct tcgataacgg
    15601 ttttctcacc tttgttatcg acttcgattt ggtaagcatc gccatttttg ccggcaaagg
    15661 aagccgtacc gaacaggctg gttactttgt ttttctggaa caggaatttc acgccgccgg
    15721 tcagtttggt tacgatggca tctttgcgct caatcatttt ggccacgtcg aattttacgt
    15781 cgccgacagt gataccgtgt tcggcaaact cgtgttgcgc tgcgtggaaa tgttcgctgg
    15841 attgcaacag ggctttagaa gggatacaac ctacgttcaa gcaagtaccg cccaatgcag
    15901 gggcattgcc tgctttgtta acgcctgcat cgacacaggc agttttgaaa cccagttgtg
    15961 cggcacggat ggcggcaacg tatccgcccg ggcctgcacc aatcactact acatcatatt
    16021 gagacatcgt gtcatccttt gcttttagca gtatctcaaa ataaaaacga tacttttcgt
    16081 ttctttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
    16141 catatctgcc gattcattcg aatcaaatgt ttcagacggt ctcaatcgtt tgttgagcct
    16201 attgtagcac gatactacat tacgctacat ctaaatatag tggattaaat ttaaatcagg
    16261 acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
    16321 ggtttaaatt taatccacta taaaaattca gacggccttt tcattaacaa ataaaaggcc
    16381 gtctgaaacg attacagatc caacaacagg cgggctgggt cttccaacgc gtctttaatg
    16441 gctaccaagg tcaatacagc ttcgcggccg tcaatgatac ggtggtcgta agacagagcc
    16501 agatacatca ttggacggac aacaacttgg ccgttttcaa ccacagcgcg ctctttagtg
    16561 gcgtgcatac ccaaaatcgc agattgaggc ggattgatga tcggggtgga catcatagaa
    16621 ccgaaagtac cgccgttggt aatactgaat gtaccgccgg tcagatcttc gatagcgatt
    16681 ttgccgtctt tggctttttt cgcgtaatca acaattgctt gttcgatgtc ggcaatgctc
    16741 atttggtcgg catcgcgcag gattggcaca accaaaccgc gtgggctgcc aatcgcgata
    16801 ccgatgtcga agtagccgtg gtacacaatg tctttgccgt caacagaagc attcacaacc
    16861 gggtattttt tcagggctgc aacagcggct ttaacgaaga aggacataaa gcccagtttc
    16921 acgccgtgtt ctttctcgaa tttttctttg tacttcgcac gcaagtccat gattggtttc
    16981 atgttgactt cgttgaatgt agtcagaatg gcgttttctt gttgagaagc caagaggcgt
    17041 tctgcaacac gggcacgcag gcggctcatc ggtacgcgtt cttcaggacg tgcgccggca
    17101 ggaagtgcaa cggcaggagc agcggctgcg gcaggtttgg ctgcggcatt ttgtacgtct
    17161 tctttcaata cgcgaccgtc acggccggaa ccttgcaatg cgttcacgtc aacaccgctc
    17221 tctgcagcca gtttggcggc ggcaggcata gcggcgttgt tttgtgcagc agcaggagcg
    17281 gcggccgggg cagcttcggc aggagcggcg gcaggtgctt cagcagcggc agtagcagcg
    17341 gtatcgatac gtgccaaaac ttggtcggca acaacggttt caccgtcttg cgctacgatt
    17401 tcaaccaata cgccggcttg tggagaaggt acttccaaaa ccactttgtc cgtttcgata
    17461 tcgatcagga tttcgtcacg ggcaactgct tcgccaactt ttttcttcca ttccaagagc
    17521 gtgccttcag atacgctttc agacagcata ggtactttta catcaataat cattttgtgt
    17581 ctccaatggc cctttcagac ggcctgttgt gtgtttttca ttctgcatat gggtgttccg
    17641 cctgagcagc ctcagacgga atgctgcttg gtttacaacg ccaaagcgtc ttcaaccaat
    17701 tgtttcaatt gagcaatgtg tttgcttgag tagcccactg caggcgatgc gctgcttgga
    17761 cgaccggcat aagacagttt ttgctcttcg ctgataacgt cttcgatgcg gtggcggatt
    17821 tggtagaacg cgccttggtt tttcggctct tcttgcgccc aaaccacaga ttttgcgttc
    17881 ggatatttcg ccagttcggc tttaacctca tcgtatggga acggatacag ctgctcaacg
    17941 cggacaatcg caacatcatc ttccagttta cgttcggctc gaccggcttc caagtcatag
    18001 taaacctgac cggcacacaa taccacgcgt ttcacgctgt cgttgcttgc gcgttcggcg
    18061 gtatcgccga caaccggacg gaaagtcgaa ccttcggtga agttttccag cgggctcatt
    18121 gcccctttga atcgcaacag gcgtttggac atgaaaatta ccagcggttt gcggtatgaa
    18181 cctaagactt gacgttgcaa gaggtggaac atttgcgacg cttcagatgg catgatgact
    18241 tgcatattgt tctcagaaca cagttgcaac cagcgttcta cgcgtgcaga agagtgctca
    18301 gggccttgac cgtcgtaacc gtgcggcagg atggtggtca gaccgcacaa acggccccat
    18361 ttggtttcgc ctgaagacag gaattggtca atcgtcactt gcgcgccgtt ggcgaagtca
    18421 ccgaattgag cttcccaaat ggtcagtttg tcaggtgcgg agcaggcaaa gccgtactcg
    18481 aacgccatta ctgcttcttc gttcaaaatg gagtcgataa ccaagaactc gcccatacct
    18541 tcgcccatat ggcgcagagg aacataagta ccgtcatccc atttttcacg tttttgatcg
    18601 tgcaatacgg cgtggcggtg cgagaacgta ccgcggccgg agtcctcacc ggagatacgc
    18661 acgccatgac ctttggttac gaggctggca tatgcgaggg tttccgccat accccagtcg
    18721 ataggctgtt tgccggatgc catggcttta cgcgcttcaa tcacacgttt tgcagtcgga
    18781 tgcagggcaa agccttccgg tacggcggta aacttctcgg tgagacgctc aatatctgcg
    18841 gcaggtaaac cggtttcgat gtgttcgcgc caatctttac cttgatattt gctccaatcg
    18901 atttgtgtgc gttggaaatt gctcaaagtt gtttgctcga cgtgttcgcc tttgtccaaa
    18961 gcatcacggt aggcttggat gtaagcatca gcttctgctt gagttaccac gccttcggca
    19021 atcagttgct cggtgtacaa agcacgcgca ccggggtgtt gcgatacttt tttgtacatc
    19081 atcggttggg tcaaggtcgg atcatcgccc tcgttgtgac cccatttacg gtagcagaca
    19141 acgtcaatca cgatgtcttt atggaatttt ttgcggtaat ccaaagcggc ttggatggca
    19201 aagcaaacgc gttcgggatc atcgccgttg acgtggataa ccggggcgga aaccattttc
    19261 gcgatatcgg tacagtgtac ggttgaacgg gtatcgcgga tatcggaagt ggtaaagccg
    19321 atttggttgt tgatgacgat gtgaaccgta ccgccggtgg tataaccgcg cgttttagac
    19381 aggttgaatg tcgcttggtt gacacccaaa ccgataaatg cggagtcgcc gtgaatcaat
    19441 acgggcaaga ctttatcgcg gccgttttcg cccaaacgtt tttgcttggc gcgtacagaa
    19501 ccttccacta ccggattgac gatttccaag tgtgacgggt tgaatgccaa agaaacgtgc
    19561 atcggaccgt gcggcgtggc aatatcggag ctgaagccca tatggtattt cacgtcgccg
    19621 ctgggcagtt tgatttcggc acggccttca aactcagcaa acaaatcgcc gggttttttg
    19681 cccaaaatgt tcaccaaaac attcagacgg ccacggtgcg ccataccgat gatgacttct
    19741 tccacgccgt ctttaccggc gttttgaatc aggtagttca aaccggcaat cgcgctttcg
    19801 ccgccttcta caccaaagcg tttttggccg acatatttgg tatgcaggta gcgttccaaa
    19861 gtttcggcag cagtcatctc tttcaagata cggcgttttt gatcggcatt gtaatgcggt
    19921 gtggacaata cgctttcaaa ataattgcgt acccagcggc gctcttcggt attgggaata
    19981 tagatatatt ccaatgcgat gtggccgcag taggtttgtt tgaggttgct gatgatttgg
    20041 gacaaaggca gtttgccgcg attggcaaaa tcgccctcgc ccatattgaa ttgaagcgtc
    20101 atatcggcat ctgacagacc gtggaatttc ggatcgaggg cttcaatatc gcgcgggggg
    20161 atacgtttga gcggatcaag ttgggctgca cccacgcctt ggatacgata ggcggaaatc
    20221 agccgtaaaa cgctgacttg ctttttcagc attgcctcat ccgcaccgcc cgcaacggca
    20281 gatgcaattt tctttttcgc caaagtaaca aatgattcgc gaatcggtgt gtgtgcgaca
    20341 tcgacagcaa ccgtccccgg ctgtttgctc aaatcggtga aatactgctt ccatttttca
    20401 tcaaccgcat cggggttttc caaaaaagcc tcgtacaatt cctcaatgta aggtgcgttt
    20461 gaaccgaaca ggtaagagaa attgagtttt tcgtccatca tggcgtgcgc tcttttcttt
    20521 aaaatataaa caaagggtag aagtccgcgt cctgacggct aaactgatgc cgcctgaaaa
    20581 gacattttat tctaccctat tttaaaccgg attgcttgtc ttgatttcat ccgactacaa
    20641 tcagacggat tcgggaaaca atccggttct ctgatattgc ctgtttgaca atatattaat
    20701 gtatgttacc tctcgcctgg cggcacataa tcgcggcgtt ccgaaccagt ataaagctgg
    20761 cgcgggcggc cgattttcag cgaaggatcg ctaatcatct cgtgccagtg cgaaatccag
    20821 cccacgctgc gcgacagggc gaagatgacg gtaaacattt cggtcgggat gcccagcgcg
    20881 gacaggacga tgccggaata gaaatcgacg tttggataca gtttgcgttc gataaagaac
    20941 gggtctttca gcgcaatctg ttccaattcc atcgccagtt tgaatttcgg actgtcttcc
    21001 aagcccaatt ccttcaaaac ttcatagcag gtttcgcgca taatgctggc acgcggatcc
    21061 atattgcggt acacgcggtg accgaagccc atcagacggt atttgcgttg tttcacacct
    21121 tccatgtatg cggcaacatt agacacatcg ccgatttcgt ccaacatttt caacacggct
    21181 tcgttcgcac cgccgtgcga agcaccccac aggcaggcga taccggcagc aatacaggca
    21241 aacgggttcg cacccgaaga ccctgccaga cggacggttg aagttgaggc gttttgctcg
    21301 tggtcggcat gcaaaataaa gatgcggtcg agcgcgcgtg ccaaaacggg attgggtttg
    21361 tagtcttcac acggcgtggc gaacatcata tgaaggaagt tttcggaata agaaagatta
    21421 ttcttcggat aattgaacgg cagaccgttt gaatagcggt agcacattgc cgcaatggtc
    21481 gggattttag aaatcaggcg gtaaatcgcg attttgcggt gttcgggatt gctaatgtcc
    21541 aagctgtctt ggtagaacgc agacagtgcg ccgaccacgc cgaccatcat cgccatcgga
    21601 tgcgcgtcgc ggcggaaccc ccggaagaac caagtcagct gttcatgcac catcgtgtgg
    21661 cggcggacgg tattgtcaaa ttctgccttt tgctcgggag tcggcagttc gccgtaaatc
    21721 aacaggtagc agacttccaa ataatcggac ttttcggcca gctgctcgat ggggtatccg
    21781 cgataataaa gcaagccttg atcgccgtcg atgtaagtaa ttttagactc acagcttgcg
    21841 gttgaaacaa atccggggtc gaaggaaaac aaacctgtat tttttgtcag cccccgaatg
    21901 tcaaccacat cgtgcccgat gctggcttcc aataccggca gctccaaacc tgcctgaccc
    21961 ggtacgttga gtttgattga tttggacata ttattgctcc tttgcaagtt ataacccacg
    22021 ttactgcatc cgcagcacgt tctttgcctt cagacggctt ttgcattttg aaatctgcct
    22081 gcaggcagat ttcagatgcc tcatgcccgt ctgatttttt caagcatcgg gataaggtgc
    22141 cctttgtccg tttccgaatg cccgttaatc aaggcaagca attcttgatc ttgaaattca
    22201 aggatttcgg aaaactcgga cagctcttta tcgctcaaat gctcgaattc tttttccata
    22261 aacctgccga agattaaatc taattccaac aatccccggc gggtttgaaa acggattttc
    22321 cgtttggcaa tatcgtcaaa aaccatcatt tcttaaacgg cccgtttcaa cataatctct
    22381 ttaatcttac cgatggctcg ggtcggattc aagtgtttag gacatacgtc tacgcagttc
    22441 ataatggtgt ggcaacggaa caaacggtat gggtcgttca gattatccaa acgctcatta
    22501 gtgatggtat cacggctgtc cgcaatgaaa cggtaagcat tcagcaaacc ggacggacca
    22561 acgaatttat cagggttcca ccaaaatgac gggcaggcag tcgaacagca ggcgcacaaa
    22621 atacactcgt acaaaccgtc caactcttta cgctcttcct gagtttgcag acgctctttg
    22681 tccgcatcaa tcggattatc gttgacaaca taaggtttga cggaatggta ttgtttgaag
    22741 aactgggtca tatccacaat caggtcgcgg ataacaggca gacctggcag aggacggatt
    22801 ttaactggct gtttcaagcc acgcagatcg gtcaaacacg ccaagccgtt tttgccgttg
    22861 atgttcatac cgtccgatcc gcaaatgcct tcgcggcagg agcggcggaa agacaaggta
    22921 tcgtcttgtg ctttcaggcg taccaaagca tccaaaagtt tcacgtcggt cggttccaat
    22981 tccaactcgt aacgctgcat ataaggcttg gcatcaacat ccgggttgta acggtaaatt
    23041 tcaaaactca ttttttccat gagaagtgtt ccttttctca tcaactgccc aatgattttg
    23101 tattataaat tcaagcagtt caatgtgggt atggttagat ttcagacgag cttatgaggc
    23161 gaaaaccgtc tgaaaacgca tcaataaacg cgcttggccg gtttgatgta ttccacgctc
    23221 aaaggcttgg tatgtaccgg tttgtaagac aaagtattgg tatctgaatg gtacagcgta
    23281 tgtttcatcc agttttcatc atcgcgctca ggatggtcgt ctgaagcgtg cgcaccgcgt
    23341 gattctttac gtgcttcggc agacaccaaa gtcgctttcg ccacttcaat taggttatcc
    23401 aattccaaag cctcgatacg cgcggtattc cacactttgc tcttgtcttt gatttcggta
    23461 cgtttcacac gctcggcaat cgccatgact tctcgaacgc ctttgctcag aatctcatca
    23521 gtacggaaca cgccggcgtg caattgtacg gagcgttgca gttcgcggcg caatgcatca
    23581 acgttttcac catcggtttg gctgtccaaa cgctcgatac gttggcgggt caactcacca
    23641 gcattagcag gcaaaggttt ccagtcgctt tgctctttga tgaatttaat catgctgtcg
    23701 ccggcagctt taccgaatac caccaagtcc agcagggagt tcgtacccaa acggttcgca
    23761 ccgtgtacgg aagcacaggc gcactcacct gcggcataca ggccttttac aggcacttcg
    23821 tattcgtctc cttgcggcac aacgacttcg ccgtggtaat tggtcggaat gccgcccatc
    23881 atatagtggg tagtcggcac aacgggaatc gggtctttaa tcggatcgat accagcgaac
    23941 tgaatggaaa tctcgcggat gcccggcagt ttttccataa ttttttctgc gccgatatgg
    24001 tcgattttca gtaagacatg gtctttgttt ttaccgcaac cgcgaccttc gtagatttcc
    24061 atcgccatcg cgcgggaaac aacgtcgcga gaagccaagt cttttacggt cggcgcatag
    24121 cgttccataa agcgttcgcc gtcggcattc aacagaatac cgccctcgcc gcgtacgcct
    24181 tcggtaatca acacgcccgc acctgccacg ccggtcgggt ggaattgcca gaattccatg
    24241 tcttccaacg ggatacctgc acgcgcacaa atacccaaac catcgccggt attcatatag
    24301 gcattggtag aagacgcata aatacggccg ccgccgccgg tagcaaacat cacagcttta
    24361 gcgtggaaaa tataaacttc gccggtttcc atttccatgg cggttacgcc gacgacatcg
    24421 ccgttttcat cacgaatcaa atcttgtgcc gtccattcca caaagaattg cgtattggca
    24481 cggacatttt gttggtacaa agtatgcagc atcgcatgac ctgtacggtc ggcaaccgca
    24541 caggcgcgtt ctaccgcgcg tttaccgtgt tcggcagtat ggccgccgaa aggacgctga
    24601 taaattttac cgctttccac acggtcaaaa ggcataccca tgtgttccaa ctcaattacg
    24661 gcttcaggcg cggcgcggca cataaactca atcgcatctt ggtcgcccaa ccagtcggaa
    24721 cctttcacgg tatcgtacat gtgccagtcc caacggtctt cctgcacatt acccagagag
    24781 gcggaaatac cgccctgcgc cgctacggta tgcgaacggg tcgggaacac tttagacaaa
    24841 acggcacaat tcagaccgga tttggataat tggagggctg cgcgtaaacc tgcaccacca
    24901 ccgccgacaa tcacggcatc aaacttgcga acaggaaaac ccatacttac ccccaaatta
    24961 ctttaattga atacaccaag cagccgacca gccagacgat ggtggcaacc tgcaaaaaca
    25021 aacgcacgcc gaagggtttg atatagtcca tccacaaatc gcggataccc acccaagcgt
    25081 gcaagaatac ggcgatgaag ctcacttggg taaatacttt tacccaagtt tgactaaaaa
    25141 atgcctgcca tgccgaatat tctttaggca gggaaaatag aaccactaaa agtgcaacgg
    25201 tataaatcaa cataataacc gcagtcgcac gttgcatcgc ccaatcgcgc aaaccgtaat
    25261 gggcaccggt caatttacgt tctaccataa caacgctccc aaaacgacag tcaaaaccaa
    25321 tgcagaagca aatacggctt tagcggtatt gcgcgcagta ttcagctcaa ggcctttgtg
    25381 tgcatccaaa aataaaaagc ggataccggc gagagaatgg tgcagataag cccacaatac
    25441 accgattaaa accagcttga ccaaaggatg ggaaacaatg gcacggtaag tttcaaatgc
    25501 agactcttga ctcagggtac cggacaggaa atacagcagg aaaggcagca taataaacag
    25561 cccgaccccg ctgatgcggt gaaggatgga aactatcccg ggtatcggca gacggatgtt
    25621 cggcaaatcc agataaacag gacgcggttt ggcagacata gtcaattcct ctgtagttat
    25681 atatcgactc aaaaaccgta aacccattgc tacactgggt tacataaacg ttaatttacc
    25741 tgttttatcc cattttgaac agtgtatttt aaaaacaaac tgatagattt tgacaaacac
    25801 gcgtggaaac agcagattaa aagaaagatt tttagttttg ccgccccctt cccgacacgg
    25861 tatcagcatc cgaccccatc cccaaaaaca aaatgccgtc tgaaaaaatt tcagacggca
    25921 ttgttttgcc atatcggtca ggctttcaat ttcgcatcgg catcgcgcaa cgcggtacgc
    25981 agaccttcct cgataacggg atggtagaac ggcatatcca gcatttgcgg aacggtcatc
    26041 ttcatttgat gcgcccatgc caacaagtgc gccaaatgtt cggcggcagg gcctacgatt
    26101 tccgcgccga taaagcggcc ggtggctttt tcggcataca ggcgcatatg gcctttgttc
    26161 accagcatca cgcggctgcg gccttggttt ttgaacgata cttcgccgat gacaaattcg
    26221 tcggcttggt attgcgcggc aacctgcgcg tatttcagac cgacaaagcc gatttgcgga
    26281 ctggtaaaca ccacgccgat ggtgctgcgc cgcaaaccgc cgccgatatt cgggtaacgg
    26341 cctgcattgt cgccggcaat cttgccttgg tcggcagctt cgtgcagcag gggcagttgg
    26401 ttggacgcgt cgcccgcaat aaagatatgc ggaatgctgg tctgcatggt cagcgggtcg
    26461 gcaacgggta cgccgcgcgc atctttttcg atattgatat tttccaaacc gatattgtca
    26521 acgttcggac ggcggcccac ggctgccagc atatattcgg caacaaatac gcctttttcg
    26581 ccgtcctgct cccaatggac ttctacattg ccgtctgcat cgagcttgac ctcggtttta
    26641 gcatccaaat gcagtttcaa ttcttcgccg aacacggctt tcgcctcgtc tgaaacgacg
    26701 gggtcggaaa tgccgccgat gattccgccc aaaccgaaaa tttcaacttt cacgcccaaa
    26761 cggtgcaatg cctgacccag ttccaaaccg ataacacccg gcccgaacac ggcaacgcgc
    26821 ttaggcagcg tatcccatga gaaaacgtca tcgttgataa tcaaacgatt gcccaaagac
    26881 tgccattgcg gcaggatgac gggacgcgaa ccggtagcaa tcacgaaact ttttgccgta
    26941 atttgagtat gctcgtcgat ttggacggta tgctcgtcga taaatttagc cgaacccatg
    27001 atgcgcttgt cggcaggcca ctcttccaca tcggcaacga caaagccgac aaaacggtca
    27061 cgctcggatt tgacgcgctg catgacctct tcgccgttga cgacgatgct gtctttgtcc
    27121 aaatgcacgc cgaacgggtc ggtatgcaat gcgtgatgac gcgcctctgc ggcggcaatc
    27181 aagagtttgg aaggcataca gcccacgcgc gcgcaggtcg tgccgaacac attgttttca
    27241 atcaggtaaa cattatccga atgtaaacgg gcattgcgaa acgcacccat accggcagta
    27301 ccgccgccga ttacgacgac atccgcttga atttttttca taatctttgt ccttccgttt
    27361 tcgtctgaaa cattgccgac acagaacata tcggtttcag acggcattta aatattttcc
    27421 caatcaaaaa catcattcag ataaataaaa acaaaatttc gattgagtgc aacaatatcc
    27481 aaattctctt ataaatatag agttattcgg agcggcagtg tagaaccgcc cgaataactc
    27541 tatccggtat atcagacagt ttaatcaaac cgccttaagc aaacagctat cagtttttag
    27601 ccaagtaagc ttccaaatct tcgctgccgc cgatgtattt gccgccgatg aagacttgag
    27661 gggcagtcat cttgccggta atggcgcgaa cggaagtaac ggttgcatct ttgcccaata
    27721 cgatttcttc gtaagacaaa cctttgtctt gcaaagcttg tttggcttta gcgcagaatt
    27781 ggcaacctgg tttagtgaaa attgccacag actcttgagc cttccaatcg ggagcaacga
    27841 attgcagcat agtatctgca tcggatactt tgaacggatc gcccagttct tcaggttcga
    27901 tgaacatttt ttcaaccacg ccgtcgttaa ccagcatgga gtaacgccaa gagcgtttac
    27961 cgaagcccaa gtcttcttta ccgaccagca tacccatacc ttcggtaaat tcgccgttgc
    28021 cgtcaggaat catgtagatg ttgtcggatt cttcttcggc agcccaagcg ttcattacga
    28081 acgtatcgtt tacagatacg cagtagattg cgtcaacgcc gttttctttg aacgcgccga
    28141 acaattcgtt gtaacgcggc aggtgtgaag aagaacaagt cggggtaaat gcaccgggca
    28201 gggagaatac gactactttt ttgcctttga acaaatcatc ggtagacaca tctttccaag
    28261 tgtcgccgac gcgggtgcgg aatactacgg aaggtacttt ttgaccggta cgatcttgca
    28321 aagccatttt aagctcctta atagattggt ttgagaaata attgtgaatc gctgggcatt
    28381 ctaccccatg caaaatgatt tgtataattt atagttcaaa taatataaat tatttaaaac
    28441 tatcaaacac aactccgatt tgcctgtttc ccacatataa agccgaatta gaaaaaaaca
    28501 agctgcaatg aaaagatttt ccggcaactg cgcccttgcg ccgcaaatca ggacaaaata
    28561 ccgtctgaaa ggctttcaga cggtatttat tcatacggtc tcggcttatt tcgccaattc
    28621 ggcacgcagt tttcgggtta cgttcatcat cacttggagt tgttccagag tttctttcca
    28681 gccgcgtgtt ttcaggccgc agtctgggtt aacccacaga cgttcaaacg gtacaacctc
    28741 gatggctttg cgcaacaggt gctcaacttc ggcttcagtc ggtacgcgcg ggctgtggat
    28801 gtcgtaaacg cccgggccga tgtcgttcgg gtatttgaat tcgccgaatg cggtcaacaa
    28861 ttccatgtca gaacgtgaag tttcgatggt aatcacgtcc gcgtccatgc tggcgatggc
    28921 cggcaggatg tcgttgaatt ccgagtaaca catatgggtg tggatttggg tactgtcttc
    28981 gcaaccggtt gacgacaggc ggaaggcttc gcttgcccaa gccaagtagg catcccaatc
    29041 ggcacgtttc aacggcaggc cttcgcggat ggccggttcg tcgatttgaa tcactttaat
    29101 accggctttt tccaaatcca atacttcgtc atttaaggcc aaggcgattt gtttgcacac
    29161 ggtgctgcgc ggaatatcgt tgcgcacaaa tgaccattgc agaatcgtta ccggaccggt
    29221 caacatgcct ttcatcgggc gtttggtcag ggtttgggcg taggttgacc aagccacagt
    29281 catcgcgttc gggcggctaa catcgccgta aatcaccggt ggtttcacgc agcgtgagcc
    29341 atagctttgt acccagccgt attgggtaaa ggcaaagcct tccagcaatt cgccgaaata
    29401 ttccaccatg tcgttacgct cggcttcgcc gtgtaccaac acatccaaat tgagtttttc
    29461 ttgctcttca atcaccaagg cgatttcttt tttcatcgcg gcttcgtaat cagcgtcaga
    29521 caattcgcct tttttgaacg cggcacgcgc ttggcggatt tcagtggttt gcgggaacga
    29581 accgatattg gtggtcggca gcaacggcag gttcagccat gcttgttgcg ctttaatgcg
    29641 ctcggcaaac ggtgatttgc gttggtcggc atctgccggt aaatcagcca agcgtttggc
    29701 cacttcggcg cggtggattt cggtgctgtt ggcgcgtgaa tcagcagcgg cttggctgtc
    29761 ggccaattcg gcggcaacgg cttcgcggcc ttggttcaat gcagttttca acacgcgcaa
    29821 ttcttgcgtt ttctgcaagg tgaaagccaa ccatgagaac aaggcaggtt tgttgacttt
    29881 cagcttttct tctaccgaca aatcgtatgg cgtgtgcagc agcgagcatg agctggaaat
    29941 ccacaagcgg tcgcccaatt tggctttcaa cggctcaagc gtggctaata cgttgtttaa
    30001 attggcgcgc caaatattac ggccatcaat cacaccggca gacaacactt tgccgtaatc
    30061 agcaaatgcg tccaattgct cgggcgcgcg caccaaatcg atgtgcaaac cggccacagg
    30121 caaggctttc agacgacctg cgtgtgcggc aaccgaaccg aaataagtgc tcaacaagat
    30181 tttggcattc acttggctca aagtggcgta aacgtcttgg tacgcatcca accacgcttg
    30241 cggcaggtca accgccaatg ctggctcatc gatttgaatc cattcggcac cggcagcggc
    30301 caattcggtc aggatttcaa cataaaccgg caataatttt ggcaacaggc tcaagcggtc
    30361 aaattcgaca gcgccttttt ctttaccaac ccacaggaag gtcaacggac caacaacggt
    30421 cggtttggct ttcaggccca aagcttgggc ttcttgcagc tgttgaacgt agtgtttggc
    30481 gttcgctttg aattcggtat cggcgtggaa ttcaggcacc aagtagtggt agttggtgtc
    30541 gaaccatttg gtcatttcga tagcgaattg gtctttattg ccgcgtgcca gttggaagaa
    30601 ttgttccaaa gacaggtttt ggctgtcgaa gccgaaacgg gcagggatgg caccggtagc
    30661 gacttgcagg tcgaggatgt ggtcgtagaa agtgaaatcg cctacagcaa cgtaatcagc
    30721 gttggcagcg gcctggtgtt tccagttttt ctcgcgcaag tctttagcaa cagccagcaa
    30781 ttcttgctcg ctgatttctt tgcgccagta tttttcttgt gcgaatttca attcgcggaa
    30841 ggcgccgaca cgcgggaagc ctgagaaatg taatgttgtc atgttgaact ctccagttgg
    30901 attttgtaag gaccggttca atccgaatcg gccggtattg taaatcagaa tggctcggat
    30961 ggggcggtgt tttgcttttt agacacctta gatgccgtct gaaactgttt gatacggctt
    31021 taagggcgca cgcctaaaat atggcaaatg gcgtaagtca gctcgctgcg gttaagcgta
    31081 tagaagtgga aatctttcac gccttcgcgg gacaggactt tgaccatatc gatggcgata
    31141 cttgccgcca ccagattgcg cgtaccttgg tcgtcgtcca aaccttcata catttgcgac
    31201 agccagcttg ggattttgac gttggttact tgagccattt taccgagctg cttgaagttg
    31261 gtaacaggca aaataccggg gacgatttcc acatcgatgc ccaacatcac gcagcggtcg
    31321 cggaagcgca ggtagcgttc cacatcgaag aagaattggg tgatgacgtg gttcgcgccc
    31381 gcatcgattt tgcgtttcaa attaatcagg tcggcttgtg cggatttcgc ttcgggatgc
    31441 acttcgggat atgctgctac agaaatgtcg aagtcggcga cggagcgtaa tagcttaacc
    31501 aagtcttcgg cataaaacgg ttttttctcg taaccgggcg gctcgtcgcc gcgcagggcg
    31561 acaatgcggc ggatgccgct gtcccaataa tctttggcaa tttggcgcaa ttcgtcggga
    31621 gaagcgtcga tgccggtcag gtgcggcgcg gcttccaagc cggtttcttg cttaatgcgt
    31681 ttgacgatgc cgtgcgtgcg gtcgcgttcg ccggaatttg cgccgtaggt tacggatacg
    31741 aacttgggat gcagggtttg cagacggtgg atggaatccc acagcatcgt ttccatttgt
    31801 tcgtttttcg gcgggaaaaa ttcaaacgaa acgttgatgt cgcctttcaa atcggaaagg
    31861 ctgttgttta acgcattgat ttcttttgcg taattcatgg ctgcgtacct tttattatct
    31921 tttatatgat atttcaggca acataataaa attgcaccga atatgagtca atttcaaaat
    31981 tttcaggttt atcatgaaaa tttctaatat attacaaaaa tagttaaatt tccttgtaca
    32041 agccttctaa atttatgata aagtttgtta tgttataaca atccaactaa ggcgatatat
    32101 gaaaccgaat attcatcctg acaattatcg aaccgtcctt tttttcgaca gcagcgccaa
    32161 tgaaggctgg ctgattcgtt cttgcgccga aacacacgga aaaacgatgg tttggacaga
    32221 cggcaaagaa tatccactgt tttcgctgga tacttcttcc gcatcccatc ccgtctatac
    32281 cggcaagcaa cgcaacgtca acaccgaagg ccgtgccagc aaattcaacc aacgtttcca
    32341 atccgtcatg tcatcgttta gaaaggacaa ataatgcagg ttctgtcttc actcaaaacc
    32401 gccaaacaac gccaccgcga ctgtcagatt gtcagacgca aaggcaaagt ttacgtcatc
    32461 tgcaaaagca atccgcgttt taaagcgcgc caacgctgac cccctgccct tcctgcttcg
    32521 gttgtaaatc ccctcctacc gcaagccctt ttcatcaaat atgccgtctg aaacagaaaa
    32581 atgcgtttca ggcggcattt tttccgtcaa tacgaatccg gaatgtgttt gaaatacagc
    32641 ataaacagat accaaaacag catcgcggcg gctgcaccga gcagtaccca tgcgctgatt
    32701 tcggcgactt cgcgcagggc gcgtttttgc ctgcggctga aaaggcggtt gataatcaac
    32761 gcggcaagtg cgaaaaagaa aaaaatacgg aaaagtctgc ctatcatgtc gggaagcggt
    32821 ggaggtcgtc tgaaaaagta tgattcgtgt gcggcggatg agggtcaaac gcgtccggct
    32881 gcttgtccga ccgtgtcaaa aaatcatgcc gtttgcgata taataacgct tttcgccgca
    32941 tttgttggaa caggatatga gtcaaaatgc ctcggtgggc attgtaacgc cccaaaaaat
    33001 tccgtttgaa atgccgctgg ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat
    33061 gattgaaacc tacggcgagc tgaatgccga aaaaaataat gcggttttaa tctgtcatgc
    33121 gctgtcaggc aaccatcatg ttgcgggcag gcattcggcg gaggataaat atacgggctg
    33181 gtgggacaat atggtaggac ccggcaaacc gattgatacg gaacgttttt tcgtggtcgg
    33241 tttgaacaat ctgggcggct gtgacggcag cagcggacct ttgtcgatca atcctgaaac
    33301 gggtagggaa tacggcgcgg attttccggt ggttacggtg aaggactggg taaaatcaca
    33361 agccgcgctt gccgactatc tcggcatcgg gcaatgggcg gcggttgtcg gcggcagttt
    33421 gggcggtatg caggctttgc agtgggcgat ttcctatccc gaacgcgtgc gccatgcctt
    33481 agtgattgcg tctgcgccga aactgtccac gcaaaatatc gcgttcaacg atgtggcgcg
    33541 tcaggcgatt ttgaccgacc ccgatttcaa cgaaggacat taccgcagcc ataataccgt
    33601 tcctgcacgg ggcttgcgga ttgcccgcat gatggggcac attacctatc ttgccgaaga
    33661 cggtttgggc aaaaaattcg gacgcgattt gcgttccaac ggctatcaat acggttatgg
    33721 cgttgaattt gaagtagaat cctatctgcg ctatcagggc gacaaattcg tcgggcggtt
    33781 tgatgccaac acctatttgc tgatgaccaa agctttggac tatttcgatc cggcggcgga
    33841 tttcggcaac agcctgaccc gcgccgtgca ggatgtgcag gcaaaattct ttgtcgccag
    33901 cttcagcacc gattggcgtt tcgcgcccga acgttcgcac gaactggtca aggccctgat
    33961 tgccgcccaa aaatccgtgc agtatatcga agtcaaatcc gcacacgggc acgatgcctt
    34021 tttaatggaa gacgaagcct atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa
    34081 ggaatgtcag caatgaacct gcgcgatgat ttgcaactga tttacgaccg gatacccgaa
    34141 ggcagccgcg tcttggactt gggctgcggc gacggcgaac tgcttgccgc attggtcgaa
    34201 cacaaaaaat gcagcggcta cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg
    34261 tcgcgcggcg tgaatgttat ccaagccgat ttggaagaag gtttaaccgc attcaacgat
    34321 caaagttttg atgtgattgt gttgagccaa accatccaag cgatgcagaa taccgaaaaa
    34381 atcctgcgct gcctgatgcg cgttgccaaa caggcgattg tcagcttccc gaatttcggc
    34441 tactggcgca accgcgtcca aatcgcactc ggcggacata tgccggtttc cgaacgtatg
    34501 ccctaccatt ggtacgacac gcccaatatc cattggtgca cgctcaaaga ctttgatttg
    34561 ttgtgcgcca aaaacaacat ccgcgtcctc gaacgcgccg tgatgacggg caacagacag
    34621 gtcaaacatt tccccaacct tttgggcagc cttgcgtttt accgcgtcgg ctaagacagg
    34681 ttgagaacct tgtcggcaat aacaggttct tcggttctat taacaacagg ccgtctgaaa
    34741 aatgaaacgg taggttttca gacggcctct ttaatactgg aaacaataga atgaaaaaca
    34801 aaacctcatc acttctctta tggcttgccg caatgatgct gaccgcgtgt tccccgagca
    34861 aagaagataa aacgaaagaa aacggcgcat ccgccgcctc gtccacggca tccgccgctt
    34921 cgtcttccgc gccccaaacc gatttgcaac cggccgcatc cgcccctgat aacgtcaagc
    34981 aggcagaaag cgcgccgccg tcaaattgca ccgacctgca ccccgccacc ggcattgacg
    35041 atctcatgca gcaaatcgcc gaacacatcg actcggactg tctgtttgcc ctttcccatc
    35101 acgaactgga aacccgtttc ggcttacccg gcggcggcta tgacaacata cagcggctgc
    35161 tgtttcccga catccgccct gaagatcccg actaccatca gaaaatcata ctggcaattg
    35221 aagacttgcg ttacggaaag cgcacgatca gccggcaggc acaagatgcc ttgatggaac
    35281 aggaacgccg cctccgagaa gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg
    35341 gacaaggcga ggagccgaaa cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt
    35401 cgagccggca caacaacgga cttggcggca atttccaata catcggccaa ttgcccggct
    35461 atctgaaaat acacggagaa atgcttgaaa accaatcact cttccggctg tccaaccgtg
    35521 aacgcaatcc cgacaaaccg tttttagaca tccattttga cgaaaatggc aaaatcacgc
    35581 gtattgtcgt ttacgaaaaa aacatctact tcaatccaaa cttggggcga agataaaaaa
    35641 atgccgtctg aaacgttttc agacggcatt tttcaatgca acaaatcaag cgcgtttgcg
    35701 gaattcgccg gtgcgggtat cgatttcgat tttgtcgccg ttttcgatgt aagacatcac
    35761 ttggatttcg gtgcccccga ccaggcgcgc ggttttcatc actttgcctg aagtatcgcc
    35821 tttgacggca ggctcggtat actcgacttc gcgtacgatg atggtgggca gttctacgga
    35881 gatggggttg ccttcgtaga aggtaacttc gcattggtct tccatgccgt caacgatgaa
    35941 tttcaacgcg tcgccgatgt tgtcggcttc gatttcgtat tggttgaatt cttcgtccat
    36001 aaagacgtac atcggatcgg caaagtagct gtacgtacag tttttgcggg acaggatgac
    36061 cacgtcgaat ttgtcgtcgg ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt
    36121 tttcagtttc atgctgactt tggcggaaga gcggccgcct ttgatgtatt cggttttttg
    36181 aacgaccata ggatcgttgc cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt
    36241 tttcattgtg attttccaat caaatcggtt gtcgtaaaac gcactattct agcgtatttt
    36301 ttgatgcttt gaaacaaagg cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata
    36361 acggctccaa tcctccgcgc cttgccgcca gccgttttga tgttgttgca ggatttgcca
    36421 acattcgagg cgttgcgtgg cggataaagc ccctccgccg ttgaggtcgt ccgaaagacg
    36481 gcggtgtgcc gacacggttt cgggcgtgta gaaaccgtgt gccttatccc aaaaggcgtg
    36541 gagtttgtcg agatggacat tctcgtcttg cgggtagatg tgccaaaaga agggtttgcc
    36601 ggcaagctgg gcgcgcacga aactgtcttc gccgcgtatc acggcgcagt cggcgaggtg
    36661 cagcagtttg tcgaagtcct gttgcggcac gaaagggatt ttgacgaggc ggacggatgc
    36721 cgtctgaaaa acatcgccgt cgttttgcag ggcatcttgc ggaataacgc cgctttgttt
    36781 gaggctgtcg atgatttgcg tccccgccag caacagtgtc atcgggctgc ctgcctgtcg
    36841 ccacatttcc agccactttg cccaaacatc gctccgatag ccgaaaagca gccattcggg
    36901 ggcgtttttt tcgggaagca tcagccgctg acgcagggct tcgctatcga aacggacggc
    36961 ttcgcagtaa tcgcgttcgc gtatcaaccc gccgcttttt tcgctgaaac ccataaacca
    37021 aaaatatttt tgaacacctt cctgcggcga aggcatcagg tgcagccttt cattgctttc
    37081 ctccgcgctc aaatattccc aattcagcca aagcggcttg tgtcggcgga taatgtgcag
    37141 cacattttcg ggcaggtcgc aggcaaaagt ttcgatgacg acatcgggaa caggcgcggt
    37201 atcaatatct gccgcatcgg aatgccaagt gcggacatga atatcctgat gaacgcaggg
    37261 aacatcgggc aaatcagggc aaagcgcacg caaggcggac acatcgtccg tccacaaatg
    37321 cacctgccaa ccgagttcgc ggtgcaaaac acgggcgagc cgccacgaaa cgccgatgtc
    37381 gccgaaattg tcgatgacct tgcaaaaaat ccaacagaca aaaggaggag tattcataga
    37441 aataacggaa acgggctaaa acgcgccgaa atagtggaac aaatacacaa tcagcaaaat
    37501 aatcggaata aacagcaaca tagtttgacg tttcaaatca aactcttgct tcaaatgatg
    37561 cttgatggtg tcgaaaagga cgaatttgaa caaaaacgtc gcttcgcagg caaggatgat
    37621 gccgttaatc agatgctggg gtttgtcttc gggcggattg gcggcgaaaa cggcaagcgt
    37681 cgccaagggt atcaggcaga cgacaagcca gcggacaagc aggtttcggg acattgcaag
    37741 gctttcaaaa aagcaaaacg ccattatcgc cccaagcgca cgccttgtct aacggatgcg
    37801 ccggagatgc cgtctgaagc cttgccgcag gaaaacaccg caccgccgaa cctttaaata
    37861 cgcccgagac gggcgttccc cgaaaaatcg gacgctgccg ccgatgcctg aggacaaggc
    37921 ggcagcaggc acgggcatat tgaaaacccg gcgcgtcggt ttgaaatttt atagtggatt
    37981 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg gaaccgattc
    38041 acttggtgct tcagcacctt agagaatcat tctctttgag ctaaggcgag gcaacaccgt
    38101 actggttttt gttaatccac tatataaagg catacggtaa acacgccccc ttgccgtcgg
    38161 cgtgcttccg caaaccaaaa tataacgtcg gcacgctttc cgctataatc cgtatttttc
    38221 caaacctgaa acgttatgcc gaccccgaaa gcctttgccc tgctcgggcc gaccgccgga
    38281 ggcaaaaccg cgctcgccct caaaattgcc gaaaccctgc ctgtcgaaat catcagcctc
    38341 gattccgcgc tggtttaccg cgatatggac atcggcacgg cgaaaccgtc cgcctccgaa
    38401 cgcgcctttg tcccgcacca ccttatcgac atcattacgc ctgttcagac atacagtgcc
    38461 gcccgttttg tcgaagactg cacgcgtctg attggcgaaa tcacggcgcg tgggaaatat
    38521 ccgctgattg tcggcggcac gatgatgtat ttccgcgccc tgacccaagg tttgaacgat
    38581 ttgcccgaag ccgatgcctg cctgcgtgcc gacttggacg aacaaaaaca aatgtacggc
    38641 ttggatttcc tctaccgcac cctgcaacag gtcgatcccg aaacagcatg ccgtctgaaa
    38701 ccgaacgaca gccagcgtat cggacgcgct ttggaagttt attatctgac cggcaaaccg
    38761 atgagcgaac acctcggcag acaaagcccc catacgctcc ccttcgattt gcataccgcc
    38821 gccctgattc ccgaaaaccg cgcccgcctg catgaaaaca tcgccctgcg tttccacctg
    38881 atgcttgaac agggctttat cggcgaagta gaaaacctgc gccgccgcta tcccggcctg
    38941 accgccgact cccccgccat ccgctgcgtc ggctaccgtc aggcatggga atatctggac
    39001 ggaaaaaccg atttcccggc atttgtcgag aaaggcattg ccgccacccg ccaacttgcc
    39061 aaacgccaac tgacttggtt gcgcaaaaca cctttagact gcgttgccga cccgttttca
    39121 gacggcactt catgcacacg cctgattgag gcggcaaaac ggtttttcgg cgcataaacc
    39181 atgctgacca caccgccgct cgcccccaaa accgtcgccg ccctgcacag gctcggtatc
    39241 cgtacgctcg aagaactgcg gcaaaacggt tcggtcaaag cctttctgct gctcaaagca
    39301 tcgggtttga ccctcacgaa aagcacgctg tggcagcttg agtcgctgct gaacggcaca
    39361 ctcccgcaag aaatcacgca acacgacaaa acgcgccttt ttgccgaatt gaaaaaccat
    39421 ccgcccgtcg ccgcctttcc gccgcaggaa gaaatggaac acttcatgcg cgaagcactg
    39481 cgccaagcag aacaatccgc cgctgacggc gaaatacccg tcggggcagt catcgtttca
    39541 gacggcaaaa tcatcgcatc ggcacacaac acctgcattg ccgactgcaa tgtcagccgg
    39601 cacgccgaaa tcaacgcctt gtcacaggca ggcagcgaaa tgcaaaacta ccgccttgac
    39661 ggatgcgaca tatatatcac cctcgaaccc tgcgccatgt gcgcgtccgc actgatacag
    39721 gcacgaatca ggcgcgtgat ctacggggcg gcagaaccca aaaccggcgc ggcaggcagc
    39781 atcgtcaacc tgtttgccga caaacgcctc aatacgcaca ccgccatacg gggcggaatc
    39841 ctgcaagaag aatgccgcgc cgtgttaagc cgtttttttc aaaacaaaag aaaaggttga
    39901 acctatgacc gtactgaccg tcatcctcgc cctcgccctg atagccgtcg gcacggcggg
    39961 catcgtttac cccgccctgc ccggattggc attgatgttt gccggaacat ggctgcttgc
    40021 ctatgccggc ggctaccaaa tctacggcgc gggcgttttg tggacggtcg gactcatcag
    40081 ccttgccggc atactggcgg actatgtggc aggcatatgg gggacaaaat ataccggagc
    40141 gggcaagctc gccgttcgcg gcgcattggc cggcagcatc atcggcatat ttttctccct
    40201 tcccggacta atactcggtc cctttatcgg cgcggcggca ggcgaactga tcgaacggcg
    40261 caatatgctt caggcaggta aagcgggctt gggtacgctg ttggggcttg tcgtcggcac
    40321 ggcgttcaaa atcggctgcg ccgtatccat cttgtttatc ctgttggtga aatacatcgc
    40381 ctacctgttt taaccctttc agacggcata ccggcaaatt gctataatgc tgtctgaacc
    40441 ctttttacaa agcaccatca tggcaaacca aagacccatc tacggcttcc acgccgtcaa
    40501 cgcccgattg tggcaaaacc ccaaatccat cgtcgaactc tacatccaag aaggcaaatc
    40561 agacgcacgc acgcgcgaag tgttggaaaa ggcggcaaac gaaaacatcc gcgtatattt
    40621 tgtcgatgcc gaccgcctca acgccatcag caaaggcgcg cgccatcagg gcgtggtcgg
    40681 atttatcgat gcctccaaaa accacgtcca cctcgaagac gtattggaaa acctcagcga
    40741 accgccgctg ctgctgatac tcgacggcat caccgatccg cacaacctcg gcgcgtgcct
    40801 gcgtaccgcc gacgcaatgg gcgtacacgc cgtcatcgca ccgaaagaca aaagcgcggg
    40861 gctgaacgcc accgtcagca aagtcgcctg cggtgcggcg gaaaccgtcc cctatatcac
    40921 cgtaaccaac ctcgcccgca ccctgcgcga attgaaagaa tacggcattt ggattatcgg
    40981 caccgatatg agcggcgagt ccgaccttta ccattgcaat ctgcccgaca gcgcggcgtg
    41041 ggtaatgggc aacgaaggcg acggtatgcg ccgcctcacg cgcgaacatt gcgacatgct
    41101 ggtgtccata cccatgttcg gcacagtcga aagcatgaac gtctccgtgt ctgccggaat
    41161 ggtattaagt gaaacacgcc gacaacgcgt attaaaaaac gaaaaagtat aagcctcaaa
    41221 aacaatgccg tctgaaacag ggattctgtt tcagacggca ttcattttca aaatcagtgt
    41281 tccaagtggt cggtatcaaa cttgaccgcc gcctcggttt tatcttcggg caagaccaaa
    41341 ttcagcaaga ctgccgtaat gccgccggcg gaaatagagt tttggaacaa gacgggcagg
    41401 tttttaaaca cttccggctc aaacgcgaca cccaagccca aaccgaccga cgttgccgca
    41461 atcaccgctt cgcgcctgcg gatgccgtga ctgaccaaaa tccgcacgcc cgcaatcgca
    41521 attaagccga acatcaaaac catcgcgccg cccaacaccg gactcggaat cgtcgtaaac
    41581 gcgcgaccga caacggggaa cagacccaac agcaccaaaa tcacggcaat atatttgccc
    41641 acatgacgcg aagccacgcc ggtcatctga atcacgccgt tgttttgcgc aaacgtcgtc
    41701 agcggcagcg aacccaaagc cgtcgcaatc accgacacca agccgtcagc caacacgccg
    41761 ccgcgcaggc gtttggtgta ttcctcgcct tcaatcggct gatcggacac cattgccgtt
    41821 gcggtcaaat cgccgaccgc ctcaaacacg ctcaacaaga aaatcgcgcc cgccacaata
    41881 aacgcgtgcc agtcaaaagc aaaaccatat ttaaacggta cgggcagcgt aaccagcggc
    41941 aggttttgca gcgcggaaaa atccaccttg cccaaaaaca gcgcgacgat atagccggca
    42001 atcagcccga ccgcaatgcc gctcatgcgc aacagcgggt ttttcatgca gttgaacacc
    42061 aacacaatca gcaacaccag cgatgccagc cccaagtttt ccatcgagcc gaacgtgccg
    42121 tccgccttcg cgccgaagcc gccgccgaaa tcggtaatgc cgacgtgtac caaactcaaa
    42181 ccaatgagca tcacgaccac gccgctgacc gtcggcgtaa tcaccttttt caaatacggc
    42241 agaagccacg ccgagaaaca caccaaaaac gcgccgacaa acgatacgcc caagagcgtc
    42301 gaaatcatcg catccttagt caaaccgccc tctttcatcc ccgcgccgag cgcaatcatg
    42361 acagtaacga acgagaaatt caccgactgg atggacagca tccccgaacc gaccggcccg
    42421 aagcggttga cctgcaaata agtgccgaca cccgacgcaa ccatcgccat cgacacgaga
    42481 tacgccgtca tctccaccgg caattccagc gcgccgccca caatcagcgc gggcgtaatc
    42541 atcggcacaa aaatcgccaa aagatgggta accgcgctca agagcgcatt accgaacggc
    42601 ggcctatcct ccaaaccgta caccaaatca ggcgcatccg cacctttgcc caactgaccg
    42661 ctcattgtat ttccttattt taaaggttga aaaatccaag cgcggattgt agcgaatctg
    42721 ccgcacggct tcaatttgca tttcagacgg catccgtctg gcccacacgt ttaccacatt
    42781 ttatagtgga ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
    42841 ggaaccgatt cacttggtgc ttaagcacct tagagaatcg ttctctttga gctaaggcga
    42901 ggcaacaccg tactggtttt tgttaatcca ctataaatcc gacggcaccg gcataaaccg
    42961 gcgaatataa taaaatgatt tcaaacagga aaaatcaatt tccaccatac ctttcggcgc
    43021 aaacgcccca catccgcaac ccgccggatt tcacacctgc ggtaaagtag gctacaatcg
    43081 gaagcattac cgccccctac accacaactg ataaaaggtt ctgtatgtta caaggtagcc
    43141 tggttgccct gattaccccg atgaatcaag acggcagcat ccattacgaa caactccgcg
    43201 acttaatcga ctggcacatt gaaaacggca cggacggcat cgttgccgtc ggcacgacag
    43261 gcgaatccgc caccctctcc gtcgaagaac acaccgccgt catcgaagcc gtcgtcaaac
    43321 acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc aaacaacacc gtcgaagcca
    43381 tcgccctttc ccaagccgcc gaaaaagccg gggcggacta caccctctcc gtcgtcccct
    43441 attacaacaa accttcgcaa gaaggcatgt accgccattt caaagccgtt gccgaagccg
    43501 ccgcgattcc gatgatactc tacaacgttc ccggcagaac cgtcgtcagc atgaacaacg
    43561 aaaccatcct gcgccttgcc gaaatcccca acatcgtcgg cgtgaaagaa gccagcggca
    43621 acatcggcag caacatcgaa ctcatcaacc gcgcgcccga aggcttcgtc gtcctgtccg
    43681 gcgacgacca caccgcccta ccattcatgc tctgcggcgg acacggcgtt attaccgtag
    43741 ccgccaacgc cgcacccaag ctctttgccg atatgtgccg cgccgcgctt caaggcgata
    43801 tcgccctcgc acgcgagctc aacaaccggc tgattccgat ttacgacacc atgttctgcg
    43861 aacccagccc cgccgcaccg aaatgggcgg tatccgccct ggtcagatgc gaaccgcacg
    43921 tccgcctgcc gctcgttccg ctgacggaag gcggacaggc aaaagtccgc gccgccctga
    43981 aagcctcagg acaactttaa atatagccat gccgtccgaa ccgttcggac ggcataacgc
    44041 aacaaacact ttaatatcca tcacacagga tgacacgatg acccatatca aacccgtcat
    44101 tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc agcaaaaccg aacagcccaa
    44161 gctcgactac caaagccggt cgcaccgcct gatcaaactt gaagtcccac ctgatttgaa
    44221 caaccccgac caaggcaacc tctaccgcct gcctgccggt tcgggcgccg tccgcgccag
    44281 cgatttggaa aaacgccgca cacccgccgt ccaacagcct gccgatgccg aagtattgaa
    44341 aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa cgctggcttg tcgttgacgg
    44401 caaatccccc gccgaaatct ggccgctcct gaaagccttc tggcaggaaa acggcttcga
    44461 catcaaatcc gaagaacccg ccatcggaca aatggaaacc gagtgggcgg aaaaccgtgc
    44521 caaaatcccc caagacagct tgcgccgcct attcgacaca gtcggtttgg gcggcatcta
    44581 ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa cagggcaaaa acggcgtttc
    44641 cgacatcttc ttcgcccaca aagccatgaa agaagtgtac ggcggcaaag acaaagacac
    44701 gaccatgtgg cagccttccg cttccgaccc caaccttgag gccgctttcc tgacgcgctt
    44761 tatgcaatat ttgggcgttg acggacagca ggcggaaaac gcatcggcaa aaaaacctac
    44821 ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa agcctgattg tctttggcga
    44881 ctacggcaga aactggcggc gcaccgcgct cgccctcgac cgcatcgggc tgaccgtcgt
    44941 cggtcaaaac accgaacgcc acgctttcct ggttcaaaaa gccccgaacg aaagcaatgc
    45001 agttaccgaa caaaaacccg gcctgttcaa acgcctgctg ggcaaaggca aagcggagaa
    45061 acctgccgaa cagccggaac tgattgtcta tgcagaacct gtcgccaacg gctcgcgcat
    45121 cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa gacgcatccg cactgttggg
    45181 caaactccat tccgaactgc gttaaaccgt tcaaaacaaa agcagcttcc aaacagaagc
    45241 tgcttttttg tgcggaactt tttacaacaa ccgaggaaaa atatcctcaa ccgcctgaac
    45301 caacgcatcc gccaaaggcg gatcgaacgc acgatgccct gcctgaacca ccctcaattc
    45361 cgcttccgga aaggctttcg acagcgccca cgcactctgc atcggcgtac acaaatcata
    45421 ccgcccctgt acgataacgg tcgggatatg ccgtattttg ccgatattgt tcaaaatcgc
    45481 cctatcgccc tgcaaccagc cgccgttgac aaaataatgg ttttccaaac gcgcgattgc
    45541 cagcgaggca taagcatctt catccacttc ctccggctcg aaacggatca gatagctttc
    45601 ccaatccgcc caagccttcg cggcagacag acacacttct tcatcttgat gaaacagcaa
    45661 tccgtgatac gcctcaatca gccggttccg ccgattttca gcaatcggcg cgacaaattt
    45721 ttgccattgt tccggataaa tccggctcac accgcccgcc tcgttcagcc acaccgtttc
    45781 agacggtctg cacaaaaata tcccgcgcaa caccaatccc tttacccgtt ccggatgggt
    45841 ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg ccgaacacca gccatttccc
    45901 gatacccagc atttcacgga ctttttcaat atccgccacc aaatcccaag tcgtattgtc
    45961 ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc tggtcgatga tgacgatgcg
    46021 gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa gcccccgcgc ccggcccgcc
    46081 atgtaaaaaa ataaccggca caccgtcagg attacccgac tcttcccaat aaatttgatg
    46141 aatttcagat acctgcaagt atccgctatg aaaaggctgt tttatctcat acatcatctt
    46201 ctcccatatt gcttctgaca atccgaacat cccgacacgc gacatcccgc acaaaacaca
    46261 agccgccccg aaaggcaaat gccgaaaaat cgcgatatag tggattaaca aaaatcagga
    46321 caaggcgacg aagccgcaga cagtacaaat aatacggaac cgattcactc gtgcttcagc
    46381 accttagaga atcattctct ttgagctaag gcgaggcaac gccgtac
//
LOCUS       DAWFKN010000006        43877 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFKN010000006 DAWFKN010000000
VERSION     DAWFKN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.006"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..709
                     /locus_tag="WGR46_000319"
     CDS             <1..709
                     /locus_tag="WGR46_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0855335.1"
                     /translation="QFAQSQPLTKTDAKDAKMPAFFAQMTAQKEDSQTMPYQPPTEAE
                     EVLEALVNRRNQLVDMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTH
                     THFDGKAQVAEQIKGIGSITTATLMAMLPELRRLSHKRIAGLAGIAPHPRESGETKFK
                     SRCFGGRSAVRKALHMATVAATRFEPLIRDFYQRLLSEGKPYKAAVTACMRKLLTISN
                     ARMRDYFAENDTAENGI"
     gene            766..1839
                     /locus_tag="WGR46_000320"
     CDS             766..1839
                     /locus_tag="WGR46_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0855336.1"
                     /translation="MRPFFVGATVLAILGALVFFISPGAVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLSAQTAGFTSLAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVLLLFMAAVSRAFLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(1885..4074)
                     /locus_tag="WGR46_000321"
     CDS             complement(1885..4074)
                     /locus_tag="WGR46_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0855337.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPRAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4213..4995
                     /locus_tag="WGR46_000322"
     CDS             4213..4995
                     /locus_tag="WGR46_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0855338.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5298..7232)
                     /locus_tag="WGR46_000323"
     CDS             complement(5298..7232)
                     /locus_tag="WGR46_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0855339.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7298..8476)
                     /locus_tag="WGR46_000324"
     CDS             complement(7298..8476)
                     /locus_tag="WGR46_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0855340.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMKDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8702..9088
                     /locus_tag="WGR46_000325"
     CDS             8702..9088
                     /locus_tag="WGR46_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0855341.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFQTYDQTKLKQNKYFKITLSKSPDHFTLTADPDPMTNEG
                     ETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9373..10419
                     /gene="adhP"
                     /locus_tag="WGR46_000326"
     CDS             9373..10419
                     /gene="adhP"
                     /locus_tag="WGR46_000326"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0855342.1"
                     /translation="MKMQAVVVNKNVAGDVEVVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10770..14255)
                     /gene="smc"
                     /locus_tag="WGR46_000327"
     CDS             complement(10770..14255)
                     /gene="smc"
                     /locus_tag="WGR46_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0855343.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEVSAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIRQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASDRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLQARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSTAEAAFKQAE
                     ATVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIFTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIAVQDKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLTQQIEALGAVNLAALQELEEVR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAESV"
     gene            14504..15226
                     /gene="lpxH"
                     /locus_tag="WGR46_000328"
     CDS             14504..15226
                     /gene="lpxH"
                     /locus_tag="WGR46_000328"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0855344.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            15496..17082
                     /locus_tag="WGR46_000329"
     CDS             15496..17082
                     /locus_tag="WGR46_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0855345.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(17251..17454)
                     /locus_tag="WGR46_000330"
     CDS             complement(17251..17454)
                     /locus_tag="WGR46_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0855346.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17570..18067
                     /locus_tag="WGR46_000331"
     CDS             17570..18067
                     /locus_tag="WGR46_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0855347.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNWETFA
                     HIGQA"
     gene            18149..19342
                     /locus_tag="WGR46_000332"
     CDS             18149..19342
                     /locus_tag="WGR46_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0855348.1"
                     /translation="MFFKHIEAALADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKHLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19746..20681)
                     /gene="hemC"
                     /locus_tag="WGR46_000333"
     CDS             complement(19746..20681)
                     /gene="hemC"
                     /locus_tag="WGR46_000333"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0855349.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20800..21315
                     /gene="ybeY"
                     /locus_tag="WGR46_000334"
     CDS             20800..21315
                     /gene="ybeY"
                     /locus_tag="WGR46_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0855350.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            21317..22141
                     /locus_tag="WGR46_000335"
     CDS             21317..22141
                     /locus_tag="WGR46_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0855351.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(22270..23649)
                     /gene="nhaC"
                     /locus_tag="WGR46_000336"
     CDS             complement(22270..23649)
                     /gene="nhaC"
                     /locus_tag="WGR46_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0855352.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23940..25163)
                     /locus_tag="WGR46_000337"
     CDS             complement(23940..25163)
                     /locus_tag="WGR46_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0855353.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            25753..26160
                     /locus_tag="WGR46_000338"
     CDS             25753..26160
                     /locus_tag="WGR46_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0855354.1"
                     /translation="MMPAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGALLC
                     AGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLAAG
                     WLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            26206..26835
                     /gene="nth"
                     /locus_tag="WGR46_000339"
     CDS             26206..26835
                     /gene="nth"
                     /locus_tag="WGR46_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0855355.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            26973..28472
                     /locus_tag="WGR46_000340"
     CDS             26973..28472
                     /locus_tag="WGR46_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0855356.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            28674..30194
                     /locus_tag="WGR46_000341"
     CDS             28674..30194
                     /locus_tag="WGR46_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0855357.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSWGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISMVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            30251..31336
                     /gene="nagZ"
                     /locus_tag="WGR46_000342"
     CDS             30251..31336
                     /gene="nagZ"
                     /locus_tag="WGR46_000342"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0855358.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLADELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            31417..31493
                     /locus_tag="WGR46_000343"
     tRNA            31417..31493
                     /locus_tag="WGR46_000343"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31451..31453,aa:Pro,seq:cgg)
     gene            complement(32014..32652)
                     /locus_tag="WGR46_000344"
     CDS             complement(32014..32652)
                     /locus_tag="WGR46_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0855359.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(32649..33020)
                     /locus_tag="WGR46_000345"
     CDS             complement(32649..33020)
                     /locus_tag="WGR46_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0855360.1"
                     /translation="MKLLFIPLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(33289..33711)
                     /gene="queD"
                     /locus_tag="WGR46_000346"
     CDS             complement(33289..33711)
                     /gene="queD"
                     /locus_tag="WGR46_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0855361.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(33718..34236)
                     /locus_tag="WGR46_000347"
     CDS             complement(33718..34236)
                     /locus_tag="WGR46_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0855362.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(34264..34923)
                     /gene="queC"
                     /locus_tag="WGR46_000348"
     CDS             complement(34264..34923)
                     /gene="queC"
                     /locus_tag="WGR46_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0855363.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            complement(35108..35302)
                     /locus_tag="WGR46_000349"
                     /pseudo
     CDS             complement(35108..35302)
                     /locus_tag="WGR46_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            35467..36693
                     /locus_tag="WGR46_000350"
     CDS             35467..36693
                     /locus_tag="WGR46_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0855364.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(36714..36995)
                     /locus_tag="WGR46_000351"
     CDS             complement(36714..36995)
                     /locus_tag="WGR46_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0855365.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(37074..37208)
                     /locus_tag="WGR46_000352"
                     /pseudo
     CDS             complement(37074..37208)
                     /locus_tag="WGR46_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(37207..37665)
                     /locus_tag="WGR46_000353"
     CDS             complement(37207..37665)
                     /locus_tag="WGR46_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855366.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(38229..38432)
                     /locus_tag="WGR46_000354"
     CDS             complement(38229..38432)
                     /locus_tag="WGR46_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855367.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSKPKEGAK"
     gene            complement(38996..39931)
                     /locus_tag="WGR46_000355"
     CDS             complement(38996..39931)
                     /locus_tag="WGR46_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0855368.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEISPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(39934..40554)
                     /locus_tag="WGR46_000356"
     CDS             complement(39934..40554)
                     /locus_tag="WGR46_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855369.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIRQVLDQLPD
                     GSPAKAAVFKANKNGTLKTAIAGVDRQTGKAVILPVKVPSKTNIRR"
     gene            complement(40648..41010)
                     /locus_tag="WGR46_000357"
                     /pseudo
     CDS             complement(40648..41010)
                     /locus_tag="WGR46_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(41106..41378)
                     /locus_tag="WGR46_000358"
     CDS             complement(41106..41378)
                     /locus_tag="WGR46_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855370.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(41566..42054)
                     /locus_tag="WGR46_000359"
     CDS             complement(41566..42054)
                     /locus_tag="WGR46_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855371.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVNFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKQDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(42051..>43877)
                     /locus_tag="WGR46_000360"
     CDS             complement(42051..>43877)
                     /locus_tag="WGR46_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0855372.1"
                     /translation="IKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVL
                     SAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEG
                     MTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATT
                     GVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAA
                     VTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNL
                     GDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAA
                     VGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVEN
                     NSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRH
                     DVQTYRQYYNQLRESRGFAVEPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGY
                     FRVDSYDPATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGTVISNVPSTPTTIRG
                     RKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT        10227 a        12007 c        10981 g        10662 t
ORIGIN      
        1 tcagtttgcc caatcgcagc cgctgaccaa aaccgacgcc aaagatgcca aaatgcccgc
       61 cttcttcgca cagatgacgg cacagaaaga agattcgcaa accatgccct accaaccgcc
      121 caccgaagcg gaagaagtgt tggaagcctt ggttaaccgc cgcaaccaac tggtggatat
      181 gcggactgcc gagaaaaacc gtctgcatca ggttcacgaa acgcaagtcg gaagcgtcaa
      241 acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa tcgacgacca
      301 cacccacacg cattttgacg gcaaagccca agtggcggag caaatcaaag gcatcggttc
      361 gataacgacg gctacgctga tggcgatgct acccgaattg aggcggctgt cgcacaaacg
      421 gatagcgggt ttggccggca ttgccccgca cccgagggag agcggggaaa ccaaattcaa
      481 aagccgctgc tttggcggaa ggtctgcggt gcgtaaggca ctgcatatgg ctaccgtggc
      541 agcgacacgt tttgaaccgc ttattcggga tttctaccaa cgcctgctgt ccgagggtaa
      601 gccgtataag gctgccgtta cggcatgtat gcgcaaactg ctgacgatat cgaatgcccg
      661 gatgcgtgat tattttgccg aaaacgatac cgccgaaaac ggtatctaaa cggcttgatt
      721 tgagttttgg tatttttgcc cgacggggtg aaaaatacag ttgctatgcg cccgtttttc
      781 gtcggcgcga cagtgcttgc catactcggc gcgttggtgt ttttcatcag ccccggtgcc
      841 gtcgtcctgc accgccaaat cttcttagaa ctcatgctac ctgcggcata tggtggtttc
      901 ctgactgcgg ctttgttgga ctggacgggt ttttcgggta acctgaaacc tgtcgcgact
      961 ttgatggcgg cattattgct cgccgcatcc gctatactgc ccttttcgcc gcaaactgcc
     1021 tcgtttttcg tcgccgccta ttggctggtg ttgctgctgt tctgcgcccg gctgatttgg
     1081 ctagaccgaa acaccgacaa cttcgccctg ctaatgttac ttgccgcgtt cactgttttt
     1141 cagacggcat atgccgtcag cggcgatttg aacctgttgc gcgcgcaagt gcatctaaat
     1201 atggcggcgg tgatgttcgt atccgtgcgc gtcagtattc ttttgggcgc ggaagccctg
     1261 aaagaatgcc gtctgaaaga cccagtattc atccccaatg tcgtctataa aaacatcgcc
     1321 attaccttcc tgctcctgca cgccgccgcc gaactttggc tgtctgcgca aaccgccggt
     1381 tttacctcgc tcgccgtcgg ctttatcctg cttgccaagc tgcgtgagct tcaccatcac
     1441 gaactcctgc gcaaacacta cgtccgcact tattacctgc tccaactctt tgccgccgca
     1501 ggctatttgt ggacaggcgc ggcgaaatta caaaacctgc ccgcctccgc gcccctgcac
     1561 ctgattaccc tcggtggcat gatgggcagc gtgatgatgg tgtggctgac tgccggactg
     1621 tggcacagcg gctttaccaa gctcgactac ccgaaactct gccgcatcgc cgtcctcctg
     1681 ctctttatgg ctgccgtctc gcgtgctttc ttgatgaacg tgaatccaat attcttcatc
     1741 accgtccccg cgattctgac cgccgccgtg ttcgtgcttt acctgctgac attcgtaccg
     1801 atctttcggg cgaacgcgtt tacagacgat ccggaataaa aatgccgtct gaaacggttt
     1861 cagacggcat cgtattgccg ataatcaagc ctcctgcgga tcgacatcca ccgaccatct
     1921 gattttgccg tccctgtttt gctgcaacac ctgcacccac aaactcacgg cacggtgcaa
     1981 atcctgtcgg gacggggatt cgaggaaaat ttgcgcgcgt tcgcgttcgg cgaggcgcac
     2041 catcagcatg ggggcggcgc caaactggga aacgcttgcg ggcaacagcg gggcgagggt
     2101 ttctttggcg gcattgagaa attccagcgc atcggcaaca cgcggcgcgt ccgcgcggac
     2161 ggcggtctga aaaccgaagg gcggcatggc gaacatttgc cgttcgttca attcgttttc
     2221 ggcaaacacg acgtagtcct gcgctttgac ggcggcgaag acgggatgtt cgggcagttg
     2281 ggtctgtatc agcaccttgc cgggcttgtc ggcgcgcccc gccctgccgg acacctgcat
     2341 cagctcggca aacagccttt ccggcgcgcg gaaatccgcg ctgtacaggc tgccgtcggc
     2401 gttcaatacg ataacgaggt tgagccgcgc gaaatcatga cctttggcga gcatctgcgt
     2461 gccgaccaga atatcgattt cgttgtcggc gatgcggcgg tacaaatccg cccagtcgtt
     2521 tttgtgcgcc gtgctgtccc tgtcgacgcg gacgacggcg gccctgggca ggaaggcgcg
     2581 cagggtttct tcgacgcgct gcgtgccgtg gccgacggcg gtcaggtctt ggttgccgca
     2641 gtcggggcat ttgtacggga tgggttcgcg gtggtcgcag tggtggcagc gcagttggcg
     2701 ggcgcgttgg tgcagcacca ttttggcgga gcagttcggg cagccgaagg tatgaccgca
     2761 gtcgccgcaa aacagcgcgg gcgcgaagcc gcgccggtta aggtacacca gcgacatacc
     2821 gcctgcttca aagttctgtt tcaaaagctg caaggcttgc ggcgagaagc cgttgtcgag
     2881 tttcagacgg cctacgttga gaatgtccac ttgcggcagt tgcgcggcgg catgggcgcg
     2941 ttcggtcagt tgcagcaggc ggtacgcgcc actttgcgcc ttgtgccagc tctccaagct
     3001 gggcgtggca ctgcccaaca cgacggggca gccgccctgc ttcgcccgcc acaccgccaa
     3061 atcgcgggca tgatagcgca attcgttgtc ttgtttgaac gagccgtcgt gttcctcatc
     3121 gaccacaatc agcccgacat catccatcgg cgtgaacact gccagccgcg tgccgatcac
     3181 cagcttcgcc tgccctaaca tcgcgcgcag ataatcctgc gtgcgcttgc ctgccgccat
     3241 ctgactgtgc aacacggcgg tcggcacgtc ggcaaaacgg ttttccaccc gcttcaaaag
     3301 ctgcggcgtg aggttgattt cgggcaacag aaacaacacc tgccgcccct gagccaacac
     3361 tttcgccatc gcatcaaaat acacctcggt cttgccgctg ccggtgatgc cgtacagcag
     3421 aaacggctgg aagcgtccca atgccgtctg aatggcatcg gaagccttct gctggtcagc
     3481 atttaataca aattcagagt gcgaagcctg cccattacac gaccttaaaa cgggcttcgc
     3541 cgcttccgtc gtttcaatcc agccttgttc agcccaatcc tcaatcaatt tcgccgcctg
     3601 cgcgtttacc tgcttcaacg ccgccatcgt catcccgccc gacagcagtg cgtcccacag
     3661 agccgctttt ttgttgaacc gcgccagcgg cggcgtttgc gccctgcccg cttcgttcag
     3721 cgcgtaaaac aacggcggct gcggcatttc caccgcgcgc gtttccttca aaccctgcgg
     3781 cagcgcggca aacaccgcct gccccgtcgg atagtggtaa taacgcgacg taaacgccaa
     3841 caaatcacgc cagctttcgg gcagcagcgg ctcgtccgaa aaggccgtcc gaacgctcaa
     3901 aatccgcgcc gtatccatat cgggcgcaat atccgtttcc cacaccatcc cgaccacggt
     3961 tttattgcgg aaaggcacaa gcacccgcgt tcccggagga agcggatcgg aatgggaata
     4021 agtcaaaagg ccgtctgaaa gcggcacgtt tacggcgatg cggtggtaga tcatacaggc
     4081 aggcaaatca tttttttgcg tattttaaca ggcggaagct attttcagac gataaattga
     4141 gcctaggcgc aatgcaggcg caacagagat ttgatacaat tccgcttttc caacacgcgc
     4201 aatcatcttt atatgaaaac caagttaatc aaaatcttga ccccctttac cgtcctccca
     4261 ctgctggctt gcgggcaaac gcccgtttcc aatgccaacg ccgaacccgc cgtcaaagcc
     4321 gagtccgccg gcaaatccgt tgccgcctct ttgaaagcgc gtttggaaaa aacctattcc
     4381 gcccaagatt tgaaagtgtt gagcgtcagc gaaacaccgg tcaaaggcat ttacgaagtc
     4441 gtcgtcagcg gcaggcagat tatctacacc gatgccgaag gcggctatat gttcgtcggc
     4501 gaactcatca acatcgacac gcgcaaaaac ctgaccgaag aacgcgccgc cgatttgaac
     4561 aaaatcgact tcgcctccct gcctttggac aaagccatca aagaagtgcg cggcaacggc
     4621 aagctgaaag tcgccgtctt ctccgacccc gattgtccgt tctgcaaacg cttggaacac
     4681 gagtttgaaa aaatgaccga cgtgacggtt tacagcttta tgatgcccat tgccggcctg
     4741 caccccgatg ccgcgcgcaa ggcgcaaatc ttatggtgtc agcccgaccg cgccaaagcg
     4801 tggacggatt ggatgcgtaa aggcaaattc ccggtcggcg gcagcatctg cgacaatccc
     4861 gtcgcggaaa ccacttcctt gggcgaacaa ttcggcttca acggcacgcc gaccctcgtc
     4921 ttccccaacg ggcgcagcca aagcggctac agcccgatgc cccaactgga ggaaatcatc
     4981 cgcaaaaatc aataatccat ccgcaaaaca aaaggcttgg aaaattccaa gccttttttg
     5041 cgggaaatgc cgtctgaaaa ccaatccgca ctgaaagtta tagtggatta aatttaaacc
     5101 agtacagcaa gccaacaaac acatcctaac ccattgaatg attgaaaata ataaactgaa
     5161 caatacgaaa cacaccgccg tcattcccac gcaagtggga atctagaacg tagaatctaa
     5221 gaaaccgttt ttgtccaata agtttctgct ccaaaatgac cggtcctgca gcttcagacg
     5281 gcatctttgt gccaacctca atcctgcgcc aatgcgtcta tcggattgag tttggctgct
     5341 ttattggcag gcataaagcc gaacgcgatg ccgattccgg tcgaacaggc gaccgccccg
     5401 ataacggatg ccgccgaaat gtccatcggg aagtcggtta caaaatgatt gaacacgagg
     5461 ctgacggcgg cggacaaacc cacgccgacc aaaccgccga tgacgcagat taacaccgcc
     5521 tcaatcaaaa actgctgcaa gatattgccg cgccgcgcgc cgattgccat ccgtatgccg
     5581 atttctttgg tgcgctcggt aacggacacc agcatgatgt tcatcacgcc gatgccgccg
     5641 actaccaatg aaatcagggc gatggaggaa atcagcagct tcatcgtacc ggtggtgctt
     5701 tcgactatct gcctgatgct gtcgctgttg ttcatgaaga aatcttccgt gccgtgccgc
     5761 gctttgagca gatcggtcag ccctttttcg gcaacctggg tattggcatt gtctttgatt
     5821 ttgacggtga tggagttggt gtggctctcg cctgtgattt ggtgcatcac cgtcgtatag
     5881 ggcgaccaaa gcatcagcac gtcggaattg ccgaaagcgt tttcgtcttt tttcatcacg
     5941 ccgatgacgg tcaaggggcg tttcctgaac aaaatggttt tacccaacgg atccgagtcc
     6001 gcaaagagtt tgtctttgac attttggtcg atgacgacga cctgcgcgtc ttctttcaca
     6061 tcgttttcgt caaacagccg ccccgtttcc agcttcagtc cgcgcacgtc gaaatattgt
     6121 tcgcccacgc cgtacaaaga agcggtcagg tcggtattgc ggtaagtcag cgtgccgccg
     6181 ctcgaagtca tgggcgtggc ggaagcaacg tagctttgtt tggcgatgat ttttgcgtcg
     6241 tctatggtca gggttttaat cctgccgctg cgcctgtcgc cgaagccgcg ccccgggaag
     6301 atgctgatgg tgttcgtccc tatcgaactg atgtcttcaa ggattttttt ctgcgaacca
     6361 ttgcccaatg cgacgacgga gacaaccgaa gcgataccga tgatgatgcc gagcatcgtc
     6421 agaagcgaac gcattttgtg cgccaatact gcttgcaccg acattctgaa ggcttcgaca
     6481 aactggtcgt aataaaacga ccacgaggct ttttcctgaa tcctcccgac attgcttgcg
     6541 gggatttctg gatttttcga ggtgtcggaa atgatttcgc cgtcccgaat ttcgatgacg
     6601 cggttggcat tggcggcgat gccggggtcg tgcgtgacca taatgacggt atgcccggct
     6661 tcgtgcagct tatggatgat ttccatcacg tttttgccgc tggcggtatc gagcgcgccg
     6721 gtcggctcgt cggcgaagat gatttctccg ccgttcatca gggcgcgggc gatggagacg
     6781 cgctgctgct gtccgcccga gagttcgccg ggcttgttgc cctctttgct tgccaaaccc
     6841 aaatcctgca agagtttgtc cgcccgcgcg gaacgctctt tgccgcccgc gcccatatag
     6901 acggctggca gcgcgacgtt atcccttgcg gtcagcgagc ttaagaggtt gtagcgttgg
     6961 aagatgaaac cgaagcgttc gcggcgcaat gccgccagct cgtcaggctg cattttggca
     7021 gtttcgatgc cgtcgattcg gtacgaaccg gaaccggcgg tatccaaaca gccgagtatg
     7081 ttcatcagcg tggacttgcc cgaaccggac tgcccgatga tggcgacaaa atcgcccttc
     7141 tctatcgaca ggctgatgtc tttcaaaata tggacgcggt tctcgccgct gccgaaatag
     7201 cggttgatgt ttttacattc gatcaagctc ataatattcc gtaaatcaat aacaaatgcc
     7261 gtctgaaacc gtttccgtgt tcagacggca tattcgttta tcggcgcggc gggccgccta
     7321 gggcgcgttc gccgctttcc tgctgctcgg cggcggttat ttcggagatg accactttgt
     7381 ccccctcttt caaaccgctt ttcacttcgg tattcatact gtctttcata ccggtccgga
     7441 tttcgcgttc cgccgccttg ccgtccgcac ccaacacgcg cacaaacgcc ttgccgccgc
     7501 gatttttcac ggtcagcgac ggaataatca gcacattttt cacaccgtcg atttcaaccg
     7561 tattctgcgt cgtcatcccc gtggcgagtt tgccgtccgg attgggcaca aacgaacggg
     7621 cataataata gaccgcattg gaagccgtat ccgtactgct gttgtagccg cccgacgaca
     7681 tcgtggtcag cccggggtcg acgctgtcga gcttcgcctt aatcggcgta tccggttcgg
     7741 acaaaatcgt aaacgaaata tcctgccccg ccttcacctt ggtaatatcg ccctcggcaa
     7801 tctgcatttt gttcaacatc atatccagat tcgccaattg gacaatcgtc ggcgtagact
     7861 gcgccgcgtt cacagtctgc ccctcttcca cgagaatcgc caccaccgtg ccgtccatcg
     7921 ttgcggtaat gcgcgtgtag cccaattccg actcggcggt attgatggaa attttgctct
     7981 gtctgattag agccttcagc tcggcaacat tggctttggc ggcggcaagc gcatcctgcg
     8041 cgctttccaa atcttcttta gaggtcgcat catccttcca caacgccgcc tgacgcttat
     8101 atttcttctc cgcactgccc aatgcaatct gtgccgacac cagcttcgcc tgatacgttt
     8161 ccaacttgga tttttccgta ttgagcgtat tggtctgcga ggtcgaattg atttccgcaa
     8221 tcaaatcgcc ctttttaacc tgttgcccga gtttgacata aagtttctta atctgccccg
     8281 atgcctgcgc gccgaccgat accaggttgg acggcgaaat ctcccctgtt gcagaaaccg
     8341 tccggctgat gtcgccgcgc ctgaccgttt ccgtaatata agcagcctgc ggttcgggct
     8401 tcagataaga ccatccgccc caaaccgctg ccgccgcgac cgccgcaaca gccgcccatt
     8461 tcatcatttt tgccatgttt tcaattctct gtatgcgtca atcccataaa tcggaagcgg
     8521 attctaccgc aagtccctgc cccatccaag gcggcaaact gccgacaata aaaataacca
     8581 tttgttttaa atggcttata caatctgctt tcccgaaaat ttcaatccgt ttacatattc
     8641 cgccatttcc gatacggcgt tataatgtcg ccgaccgaca aacaaatgga aacacattgc
     8701 tatgaaaaac gttcaaaaag gctttacgct gctcgagctg atgattgccg tcgccatcct
     8761 cggcatcctg acgctcatca cctacccttc ctataaaacc tacatccggc gcgtccgcct
     8821 gtcggaagtc aggacgaccc tgctgcacaa cgcgcagacc atggaacgtt actaccgcca
     8881 aaaagggacg tttcaaacct acgaccaaac caaactgaaa caaaacaaat atttcaagat
     8941 taccttaagc aaaagccccg accacttcac ccttacggcc gaccccgatc cgatgaccaa
     9001 cgaaggcgaa acctgcgtcg ttacgctcaa cgacggcggt accattgccg cttccggtac
     9061 aaaccaatcc tgccccggct tcgactaatc ctccgccgaa gcccgcaaac ccgtaaagat
     9121 gccgtccgga cacagcttca gacggcatct ttacgggttt gcccatccgg ttttttcaaa
     9181 cttgaaacct gccgcccctt atccggcaac cgccccgatg ccgcgccttt ccgagctgtt
     9241 ataatataac atataatcta tatttttcaa caacatacca cttgacctgt gagatgcgga
     9301 agcgtatgct tacccttata tcggacaaca ttccgtttac aggttttttt gccgtaacta
     9361 aggagcgcaa tcatgaaaat gcaggcagtt gttgtgaata aaaatgtagc gggcgatgtg
     9421 gaagtagtcg aacgcgaggt tcgcccgttg gaatacggcg aggcattggt cgaagtcgaa
     9481 tattgcggcg tgtgccacac cgacctgcac gttgcggcag gcgactacgg cgaaaaaccg
     9541 ggccgcgtgc tgggacacga gggcatcggt ttggttaaag aagttgccga cggtgtgaaa
     9601 aatctgaaag tcggcgaccg cgtcagcatc gcctggctgt tccaaagctg cggctcgtgc
     9661 gaatactgca acaccggccg cgaaaccctg tgccgctctg tattgaacgc tggctacacc
     9721 gccgacggcg gcatggcgac ccactgtatc gtgagtgccg attacgcggt caaagtccca
     9781 gaaggtttgg atcctgcgca agcttcgagc attacttgtg ccggtgtaac cacttataaa
     9841 gccattaaag tttccggcgt tcgtccggga cagtggattg ccatctacgg cgcgggcggt
     9901 ttgggcaact tgggggtcca atacgcgaaa aaagtattcg gcgcgcacgt tgtcgccatc
     9961 gacatcaacg acgacaagct ggcatttgcc aaagaaaccg gcgcggattt ggttgtcaac
    10021 gccgccaaag aagacgctgc caaagtgatt caggaaaaaa ccggcggcgc acacgctgcg
    10081 gtcgtaaccg ccgtatctgc tgccgcattc aactctgccg tgaattgcgt ccgcgcgggc
    10141 ggacgtgtgg ttgccatcgg actgccgccg gaatcgatgg atttgtccat cccgcgtttg
    10201 gttttggacg gcatcgaagt ggtcggctct ttggtcggca cgcgcaaaga tttggaagaa
    10261 gccttccaat tcggcgcgga aggtttggtt gtgccgaaag tccaactgcg tgctttggat
    10321 gaagcacccg ccattttcca agaaatgcgc gaaggcaaaa tcaccggccg tatggtgatc
    10381 gatatgaaaa aagaatgcgg ctgcggtcat caccactgat ttgacgtggc gatacacatc
    10441 gaaatgccgt ctgaacgctg ttcagacggc attttttatg gatttggatt tgattttaat
    10501 ctgttctgtt tgaaataccg tctgaaaacc cgtatcgcaa cacgtcatta tagtggatta
    10561 acaaaaatca ggacaaggcg gcgagccgca gacagtacaa atagtacgga accgattcac
    10621 ttggtgcttc agcaccttag agaatcgttc tctttgagct aaggcgagcc aacgctgtac
    10681 cgatttaaat ttaattcact ataccgtctg aaaacccgta tcgcaacacg tcattaagaa
    10741 cggcaagatt caaccgttct gcaacccgtt tagacggatt ccgccatttc caacgcctgt
    10801 ttgatgtcca cggcgacgac gcgcgatacg cctttttcct gcatggttac gcctacgagc
    10861 tgctctgcca tttccatggt caggcggttg tgggagatgt agaggaactg ggtttgcgcc
    10921 gacatttctt tgaccaggtt gcagaaacgc gaggtgttgg cgtcgtccag cggggcatcg
    10981 acttcgtcca aaaggcagaa gggggcgggg ttgaggctga acagggcgaa cacgaggctc
    11041 atggcggtga gggctttttc gccgccggag aggaggtgga tggtgctgtt tttcttgccg
    11101 ggcggacgcg ccatgatgga tacgccggcg gtcaggaggt cgtcgcctat cattttgagg
    11161 gtggcttcgc cgccgccgaa cagggttggg aagaaggttt ggactttgcc gttgacggca
    11221 tcgaaggttt ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat ggcttcttcc
    11281 aaaagggtaa tggccgcctg cacgtcttcg ctctggctgc ggtagtagcc gtcgcgttcg
    11341 cgcacttctt cgagttcttg cagggcggcg aggttgacgg cgccgagggc ttcgatttgc
    11401 tgggtgaggc tgccgatact gctgttcaat actttcggcg attctttcgc caaggcttcg
    11461 agcgcatcca aatcggcggc gcgttcggtc aggttttgat ggtagcgttt ggcgttaatc
    11521 agggcttcct gctgctgcaa cagggcggtt tgggtggctg cctgaagctg tggcaactgg
    11581 gtttgcaggg tttgtacgcg ggcgtattgt tccctgccct gcgcctgaat ctgcgcgagt
    11641 ttgtcctgca cggcgatgta ctcttcatcc aaagtttgta cggcttcgct taattcttca
    11701 agcttgatgt gctgctcgtc gttttggaac tcggtttcat aggcgaggac aagctcttgc
    11761 tggcgttcct gccagtctag ggtttgctgt tcgagtcggg caatttgctg ccggtaattt
    11821 tgtttttgct ggttgagctt atggacggcg acttcggcca gcccgtattg gcggttggct
    11881 tccaacaggg caagctgcgc ctgtttcaga cggccttgct gctcttggcg gctgtgcgcg
    11941 gtggtttgct gttggtgttc gagttcggcg gcggcttcct gcaaagtaaa gatgtcgtct
    12001 gaaagcccgt cggacgtgtg ttgcaacacg gtctgctctt ccgccaactg cgccagttcg
    12061 cgctcgatgt gttcgcggcg gatttgccct tggttggtac gcgccagaag ttcggcggcg
    12121 cgttgctgcg cctgactgta ctggcgcgtg tgctgctgtt gctgctgcat caggttttta
    12181 tgttgcactt cggacgagcg tacagttgcc tccgcctgtt tgaacgcggc ttcggcggtg
    12241 gagagttcgg gggcgaggtt gtccagttcg gacgcgatgc cgtcgagacg ggctttttgg
    12301 gcaatcagac tttcctgcgc gggtttggca tagagcagga cgctgacttt atcgacctga
    12361 tggccttcgg gcgtgagcca gatttggtgt gcgcccaaat cgctttgatg cgcgagggcg
    12421 taactcaaat caggcgcaca caatacgccg tcgagccagt ggtgcaatgc cgtctgaaac
    12481 ggcggctgcg cttggatttg gttcagcaat gcctgtacgg gcagggattt tttgatgccg
    12541 ccggagagac cgtctgaaag ccacgccgcc tgcccctgcg gcaaaggcgc ggggggaacg
    12601 aaaccatgcg gtacgctacg ggcttgcagg cgttcggcaa ggatgacgga caaggcgtgc
    12661 tgccactcgg cgggcgcggt gatgtgttgc cacagttgcg gcgcggcggc gtggtcggtt
    12721 gcctgccaga aatcggcggc ttcctgctgt tgcgacagga tttgcgacaa cgcctgctgc
    12781 tgcgcctgca aggtgatgtg ttgctgcttc aggctttgga agcggtctga ggccgtctga
    12841 aacgcctcgc gggcagcgtg taaggcttct tcggcggcaa tgatttgttc ttcgtaatgc
    12901 tcttgccggc tttgcaacaa ggcggcggct tcctgcgcgg cggcggtttc ggcttcgtcg
    12961 ggcaggttca gggcttggtt ttcctgtttc agacgacctt tgcgctcttc gtgcttggca
    13021 acggtttgtt cggcatgggc aagctgctgc tgcttcaacg ccagttcgcg gcggatgcgg
    13081 tttgcctcgt cctgctgggt ttggaaggcg gcgttgagcg tagcttgggc ttcttccaat
    13141 tcgggcagac gctcttcgtg ttcggcaacc tgcatcgccc attccgccag ctcggtttgc
    13201 ttttcttcga cgtataactc gttttcttcc agctgcacgc ggatttgctg ctgctcctgc
    13261 cggatgcgtt gcatctgcgc ctgcgccgcc tgcttgtcgc gttcgatgcg ttggtgcagg
    13321 ttttgctgat ggcggatttg ttcttccaaa cgggcaatct gctcgcgcaa cacaccgcgt
    13381 ttgttgccca gttcgtgtac cgcctgctgc tgcgactgtt cggcggtctg caaggcgtgt
    13441 acttcgtcgt ttaacgcctg aacctgcgcg gcggtttcgt cctgctgcgc ctgcaaagac
    13501 tgatgctgcg cggtcgcctt gtcggcggcg gcaagcgatt gctgccattg ggcgtaatcg
    13561 agcaaatcct gttgctggtt cagctgcgcg gtcagggatt tgtagcgttc ggcggtttcc
    13621 gcctgtttct ccagcttttc cacctgacgc gctaactcgt tctgcaaatc gcccaaacgc
    13681 tgcaaatgct cgcgcgtgtc tttcagacgg ccttccgtct ccttacggcg ttccttatat
    13741 ttggacacgc ccgccgcttc ctcgatatag gcgcgcaatt cctccggccg cgcttcgatg
    13801 atgcgcgaaa tcatcccctg ctcgataacg gcataaccgc gcgcgcccac gcccgtaccc
    13861 aaaaacaaat cggtaatgtc gcggcggcgc acggtctgat tgttgatgaa ataagtcgat
    13921 tcgccctgac gcgtcagctg ccgcttgatg ctcacctcgg catactgccc ccacgccccc
    13981 tgcaaactgt ggtcgctgtt gtcaaatacc agctccaccg aagccctcgg cgcaggacgg
    14041 cgcgtcgccg caccgttaaa aatcacgtcc tgcatactct cgccgcgaag ctgcttcgcc
    14101 gaaacctcac ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc gcaaccgttc
    14161 ggcccgataa ccgcgacaag ctgccccgga acatgaatcg tggtcgggtc ggtaaaagat
    14221 ttgaagccgg agagtttgat gtgggtcagg cgcataatgg tcggaaaaaa taaaaaagaa
    14281 gcgtatttta acggaaatcc cgccgcacca ccccatatct tgccggcaaa accttaccgc
    14341 atcccgcacc cgcgatgccg tctgaagcct tcagacggca tttttaacgc gcccgaaccc
    14401 cggttccgcc aatgcccgat gcttggcgcg gcatttgccg tattgcccca ccgccgggcg
    14461 cgttataatc cgccccgaca tccgcccgat tggaaacctg cctatgaaac ccgcctattt
    14521 catttccgac ctgcatttga gcgaaaagca gcccgaactg accgcgctgt tgctgcgttt
    14581 tttacgttct tccgccgcca ggcaggcgcg ggcggtttac attttgggcg atttgtttga
    14641 tttttgggtg ggcgatgacg aagtttccga gttgaatact tcggttgcgc gtgaaatcag
    14701 gaaattgtcc gacaaaggcg ttgccgtgtt cttcgtcagg ggcaaccgcg acttcctgat
    14761 cggtcagaat ttttgccggc aggcgggcat gacgctgctg ccggattact cggttttgga
    14821 cttgttcggc tgcaaaaccc tcatctgcca cggcgacact ctgtgtacag atgacagggc
    14881 ataccaacgt ttccgcaaaa tcgtgcatcg gaagcggctg caaaaactgt tcctaatgct
    14941 gcccctgaag tggcgcacgc gccttgccac caaaatcagg cgtgtcagca aaatggaaaa
    15001 acaggtcaag cccgccgata ttatggatgt caatgccgcc tttaccgcgc ggcaggtacg
    15061 cgccttcggt gcggaaaggc tgatacacgg acacacccac cgcgagcata tccatcacga
    15121 aaacggcttt acccgcatcg ttttgggcga ctggcataac gactatgctt caatcctccg
    15181 cgtggacggg gacggcgcgg tattcgtgcc gctggaaaaa tgctgaaaat gccgtctgaa
    15241 gcctttcggg cggctttttt ttacacgccc ctttcccaca ctccctccgc cgcatcatca
    15301 ccatccttaa cccgcattaa tgggcgttat cgataaaatg cttgaccgat tacataagat
    15361 tacgtaaagt gtgcaaacgc ataacaattg gtcttaccaa tttatctttt aaccaaaata
    15421 tcgggtgtac gggatttgaa cgtctttcca aacctcgacg cgccttatga tgcaaatgcc
    15481 taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct gctgatttgg
    15541 ctgatggtga aaaaaaacag tatgccctcc tacgtcgcgc tgccgattac cgcagtgctg
    15601 atttacgcca tcaaactttt ctacttcgac gatgcgggca tgctgctcaa cgccaccgcc
    15661 gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc gattatgttc
    15721 aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct ggcgaccatc
    15781 agcccgaacc ccgtagcgca actgatgatt atcggctggg cttttgcctt tatgattgaa
    15841 ggcgcatccg gcttcggcac gcctgccgcg attgccgcgc cgattctgat gagcttgggc
    15901 ttcaacccgt tgaaagtggc gattttcact ttggtgatga actccgtccc cgtctctttc
    15961 ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag tgccgaagac
    16021 atcctcgcca tcggcaggca gaccggcgta atgcacttct tcgcaggttt cgtcatcccc
    16081 gtcatcggct tgggcttcat cgtaccttgg tctgaaatcc gcaaaaactt gggcttcgtc
    16141 gccattgccg tcttctcctg caccattcct tatgtcgcat tggcgatggt caacgaagaa
    16201 ttcccgtcgc tcgtcgccgg cgcgatcggc ctgatggtgt ccgtattcgc cgccaaccaa
    16261 ggctggggct tgagcaaaga ccacgccaaa gacccgaatg ccgaaaaagt gccgttcgcc
    16321 caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt ggttacgcgc
    16381 atcaaacagc tcggcatcaa aggcattttg accagcaaag aagaatggtt cagcttccaa
    16441 ctgccgtttg atttgtccaa aatcaccgtc agcgactccc tgacgattac tttcggcaat
    16501 attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc ttggattccg
    16561 tttgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa agatgcctgg
    16621 acgatttatg ccgtaacttt caatcaaacc aaaaaaccgc tgcttgccct gatgggcgcg
    16681 ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa aatcatcggt
    16741 aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tctcgccgta tctgggcgcg
    16801 attggcgcgt tcttctccgg ttccaacacc gtgtccaacc tgaccttcgg cccgattcag
    16861 cagcaaatcg ccttggatac cggcctgtcc gttaccctga ttctggcgtt gcagtccgtc
    16921 ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg taccgtattg
    16981 gatgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat ggcgatttac
    17041 ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg ccaccatcca
    17101 tagcggcagc acgcggggaa cggaaaccgc cgttccccgt ctgggcggcg cgtcgggcag
    17161 aatcccggtg ccggaacaag cccgccccgc caaacaaatg ccgtctgaaa ccggaaaagg
    17221 cttcagacgg catttttccg ccgcttgcgt tcaggcgtaa tgatgatgcg gcatcttgcc
    17281 gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt ctccttcgca
    17341 caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc ccgagcaata
    17401 cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt ccatttgatt
    17461 ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa ggatattttc
    17521 ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca tgactgcgct
    17581 tacccttccg gaagacatcc gccaacaaga accatccgtt ctgctctata ccctcgtttc
    17641 cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc tgtccgacga
    17701 ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag gcggcttcgt
    17761 gcaactcatc gcctccggtt acggcgaata tattttccgc aacccgcttg ccgacagcct
    17821 gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac aaagccaaag ccctctacga
    17881 acaacacggc gaaaccatcg aaacgctcgc cgacggaggc gcagacatcg aatccctgcg
    17941 caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg ccgaacaaga
    18001 cctgcccctg cttgcagaac acatcctgtc aaactgggaa accttcgccc atatcgggca
    18061 ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca aacccgaatg
    18121 accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag ccgccctcgc
    18181 cgatccgatt ctcggtttgg gcgaagcatt caaagccgaa acccgccccg aaaaagtcaa
    18241 cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccattgtca aagccgtcaa
    18301 agaagccgaa aaacacctgt tggaaagtga aaccaccaaa aactacctca ccatcgacgg
    18361 cgttgccgat tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc acgaaatcat
    18421 cgccagccgc cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat tgcgtattgc
    18481 ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca atccgacctg
    18541 gccaaaccac aacgccatcg ccaaggcggt cggtatccaa gacaaacctt atcgttatta
    18601 tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga gccaagcgca
    18661 aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca tcgaccctac
    18721 gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt tgccgctgtt
    18781 tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg gcttgcgcgt
    18841 gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa acttcggtat
    18901 gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa cagcagcccg
    18961 cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg cttcacacgg
    19021 tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat ggattgccga
    19081 actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg ggttgctcaa
    19141 agccaaaggt gcaagccaaa actttgattt cattatcaaa caaaacggta tgttctcttt
    19201 cagcggcttg actcccgagc aagtcgaccg cctgaaaaac gagtttgcca tttatgccgt
    19261 ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt atctgtgcga
    19321 aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg aaccttaacc
    19381 agttcagacg gcatctgttt tttgttaaaa aacaataaac cataacaatt tcaaacaagc
    19441 taaaaaacat ctataaaatc aacccaatat gatgaactca aataaaaaat acgaatatca
    19501 tcattctcaa attcaaaatt tgaacgccac agtattatcc gaagccacaa aaacctttcc
    19561 gccgtcattc ccgcgcaggc gggaatccgg gaacccaatg ctgcaagaat tgattagaaa
    19621 caactgaaac cgagcaaact ggattcccgc ctgcgcggga atgacgaaat aaggcgagta
    19681 aaactgatga aacaaattga ttaaaaacaa taacttatat tattttattt aaattttcga
    19741 taaaatcaat tttccgtatt caatactgct ccaatcaatt cccgcgcacc gtcgtccgcc
    19801 aatttcttag cgacagcgcg tccgagcgcg tcggcatatt cggcaggggc ttgcgcgtcc
    19861 gcctgcaaca caaccgaacc gtcggggtgt ccgaccagtc cgcgcaaggt cagcaagccg
    19921 ttttcttccg tgcaatatgc ggccagcggc acttggcagc ttccgcccaa agcgcgcgcc
    19981 aaagctcgtt cggccgtaac gcaggcgtgt gtggtatcgt ggtttagcgg ttttaaaacc
    20041 tcatacaaat cttcgcggtg cgcggcaatt tcgataccca atgcgccttg tccggcggca
    20101 ggcaggctgt cggattccga caaaatcatg cggatgcgtc cgtccaattt cagacgctgc
    20161 aaaccggcgg cggccaaaat aattgcgtcg tattcgccgt tatcgagttt ggacaaacgg
    20221 gtttgcacat tgccgcgcaa aggtttgata agcaaatgcg gatagcgcgc gcgcaactgg
    20281 gcttcgcggc gcaggctgga tgtaccgacg acagcgcctt tgggcatttc ttccaaacgc
    20341 gcgtattggt tggacacaaa cgcgtcaaac ggattggcgc gttcgccgat ggcggcaagc
    20401 gcgaaacctt caggcaaatc catcggcacg tccttaatcg aatgcaccgc caaatcggcg
    20461 cgcccgtcat ataaagcctg ttccaactct ttgacaaaca agcctttacc gccgactttt
    20521 gacaaagttt tgtccaaaat ctgatcgccg cgcgtggtca tgcccaaaat ctcgacttcg
    20581 caatcgggat acagagcctt cagacggcct tggatatgct ttgcctgcca catagcaagc
    20641 aggctttcgc ggctggcgat gacgagtttt ttcgggttca tgttcaatgc gttcaaagaa
    20701 tggaaacggc gtgaagtcta tcatattttg aacgtttctt cagacggcac cgtcaggtct
    20761 atgttatatt tacacccaca aaacacgaaa cccgccacaa tgaaacgcac caaaaaatac
    20821 cctttcttaa cgttgcagcg gcaacgtttc catttgaact ttgaaaacgc ctcttccgcc
    20881 gccggcatcc cagccgaacg cgatttctac cgctgggcgt ggtctgcctt gaaaaatgaa
    20941 taccgccgcg ccgacatcag cctgattctt ctggacgaag aagaagcccg agcctacaac
    21001 cgcgactacc gcggcaaaga ttacgccacc aatgtattga gtttcgcgct caacgaaggc
    21061 gaaatcctgc cctgccaagt ttcggaaaaa ctgtacggcg atttgattat ctgcccgcaa
    21121 gtggttttga aagaagccgc cgaacagggc aaaacgcccg aacggcattt tgcccacctg
    21181 accatacacg gcgttttgca cctgatgggc tacgaccaca tcgaagacga tgaagccgaa
    21241 ataatggaag ccgaagaaat ccgcctgatg ctggcggcag gcttccccaa cccctaccaa
    21301 gaggacgaat attaaaatgg acggtacgca gtcgaaaccc aagtttttcg aacgcctgat
    21361 ctcccgactt gccggcgagc ccgactccgc cgaagacgta ttgaccctgc tgcgccaagc
    21421 gcacgagcag gaagtttttg atgcggatac gcttttaagg ttggaaaaag tcctcgattt
    21481 ttccgatttg gaagtgcgcg atgccatgat tacgcgcagc cgtatgaacg ttttaaaaga
    21541 aaacgacagc atcgagcgca tcaccgccta cgtcatcgat accgcccatt cgcgcttccc
    21601 cgtcatcggc gaagacaaag acgaagtttt gggcattttg cacgccaaag acctgctcaa
    21661 atatatgttc aaccccgagc agttccacct gaaatccgtc ttgcgccctg ccgttttcgt
    21721 tcccgaaggc aaatctttga ccgccctttt aaaagagttc cgcgaacagc gcaaccacat
    21781 ggcgattgtt atcgacgaat acggcggcac gtcgggtttg gtcacctttg aagacatcat
    21841 cgagcaaatc gtcggcgaaa tcgaagacga gtttgacgaa gacgatagcg ccgacaatat
    21901 ccacgccgtt tcttccgaac gctggcgcat ccatgcagct accgaaatcg aagacatcaa
    21961 caccttcttc ggcacggaat acagcagcga agaagccgac accattggcg gcctggtcat
    22021 tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc cttatcggcg gtttgcagtt
    22081 caccgtcgca cgcgccgaca accgccgcct gcatacgctg atggcgaccc gcgtgaagta
    22141 agcaccgccg tttctgcaca gtttaggatg acggtacggg cgttttctgt ttcaatccgc
    22201 cccatccgcc aaacataaaa atgccgtctg aaaccgttgc aagtttcaga cggcatttcg
    22261 cttatccgct tattttttgc tcaaagtcag ccccgtccaa ccgaataaca gggtcaaagc
    22321 caaactcaaa tagcagaaaa aggcatacgg cagatattcc caaaccggca cgcccagcgc
    22381 gtggctgatg aacacgccgc atacgctcca cggtacgagc gggttaatca ccgtccccgc
    22441 atcttccagc gtccgcgaca gattgcggga atgtagtccg agcctgtcgt aaacgggttt
    22501 aaacgtttct ccagaaagca ggatgctcaa atattgttcg ccaatcagga aattaacccc
    22561 gaccgaagtc atggcaacgc tgaacgtcgc gcgtccggca ttcgtcaaga aggtacggat
    22621 ggcctccaac agggaaggaa tcacaccgag cgcaaacaac agtccgccca aactcatccc
    22681 gagaatcacg atggtttgcg tgaaaaacat actttccaaa ccgccgcgcg aaatcagttt
    22741 gacaacatct ttaaacgctt cgccttcgag tttgtagccg ccgtaaaacc acgcaccgag
    22801 ctgacgcaga tcgggcgtgc tgtgcagata cgttacagca acggcaacca tgacggtaaa
    22861 gagcatggcg acgacggcgt tgatgcgcat caatgccaaa atgaccaaca gcgcaaacgg
    22921 aatcagcgaa tagccgtgca ccaatcccgt ggcttcaagc tggctgcgga aggattcgac
    22981 gctgttcaaa tcctgcgcgg cgacattcgg caaaagccaa agcatcagtg ccgcactaat
    23041 gagccacgcg gggatggtgg tgtacatcat atttttgatg tgctcaaaca agtcgatgcc
    23101 gacgatggac gcggaaatac ccgtcgtatc cgaaagcggg gacattttgt cgccaaaaaa
    23161 tgcgcccgaa acaatcgcgc ccgccgtcat cgccatatcg gcctgaaacg ccgccgccat
    23221 ccccataaag gcaacgccga cagtggcgca ggtggtcagg ctgctgccga tggacacgcc
    23281 gatgacggaa cacagcgcga aggcggaaaa ataaaaataa gtcggagaaa tcagcccgaa
    23341 accgtaatac atcagcgtcg gaatcgcacc gctcatcatc agtgcgctga ccatcagccc
    23401 gatgaagaaa aacaggtaaa tcgcgcccat accctgattc aacgcgccta tcatgccctg
    23461 ctgcatatcg ttgtatttca aaccgcgcgc caagccgtac aaaatcagca cgacgatggc
    23521 ggcaataatg gacatatgcg gcagccactc caatgaaatg atggtatagc ccatcgcggc
    23581 aatcagagcg acgacgacgg caagtgcctc accgcgcggc atatcgagta aggatttgaa
    23641 agcaaacatg attctttgcc cgatttgata taaagttaac ggattctaac acaccatacc
    23701 gaatccgccc aacgttttca gacggcattt tagcgtttta caaaagccgg acagaaacaa
    23761 aacggcaatt ttctcaattt aaattaattt ttttgaaata taatttttta tttttttgtt
    23821 tgttggcata taagactttt atggattgaa gtattaaaac ctcggcacac acgccgcaaa
    23881 caccggtacg caaaaatgcc gtctgaaaga tgttcagacg acatttctca agaaaaagat
    23941 tatttgtccg ccctatatcc gtacaccgca aaaaacacga tatacagata acagatgacg
    24001 gaaacgacaa acgaagacat cagggtgtaa gtatctgcca cccagccctg cacgacagga
    24061 acgaccgcac cgccgacaat cgcggtacac agtacaccgg aagccgcgtt ggtaaatttt
    24121 cccaatcctt tggttgccaa agagaaaatc gtcgggaaca taatcgagtt gaaaaaaccg
    24181 atggcaagca gcgaccacat cgccacatcc gcattgccgc tacccgtcgc catcgcgacg
    24241 gcaagcagta cgaccgcagc cgatgcgtta aacgccaaat aacggttggg cgcgaatttc
    24301 gccatcaccg ccgaaccgag gaaacgtccg accatcgcgc cgccccaata gaacgacagg
    24361 taatgcgcgg cagaagcatg atccagccct ttcagataac ccaatacgtt gaccatcaac
    24421 gaaccgatag acacctccgc gccgacatag cagaaaatgc ctgccgtacc gaacacgaga
    24481 tgtttgtatt gccatacgct ggttttgccg tcgtggttgt gcgcgctttc ctcggcggca
    24541 attttgcgcg cgtcgggcag ccggatcatt ttcacgaaaa cggcaaggat aatcagcagc
    24601 cccgccagtc ccaaataggg aatctgtacg gaagaaatct gttccgcctt gctgacggtt
    24661 tgggttgcgt ccgccagaat caggaacgcg ccgatttgcg gcgcaatggt cgtacccaaa
    24721 gcgttaaacg cctgaaccag cgtcagtgtt gccgattcct tgccgggttt cgccagcagg
    24781 gtaacataag gattaccggc gacctgaagc agcgttacgc cggaagccaa aataaacaac
    24841 gcgcccaaaa ataccgcgta agaatggcta cccgcagcag gataaaacag caggcatccg
    24901 accgccgtca gcagaaagcc gccgataacg ccgtttttgt agccgacttt gccgacaaaa
    24961 gcccccatcg ggatggacat caccgcatag gcggtaaaga aacagaattg gatcagcatc
    25021 gcctgaacgt aagacaggtc gaaaatttct ttcaaatgag ggataaggat gtcgttcatg
    25081 caggtaataa aacccatcat gaagaacagc gtggtcaaga cgaccaatgg ggacgtatga
    25141 ttgttttgtg attgcgcaga catgatttgc ctttcgtggt gtcggaatat tacccgtttc
    25201 aagaaaatag aaattgacat ttcttaacgg caatgcaaat catactgata tttgaacatt
    25261 ttgcaaactt tatcggatgc aggcaatgcg ccgatctgaa attcaagcag cagaaattaa
    25321 tattttacag ttgcttgcat agattttaat agattttaaa aaattccggc ggacttgcaa
    25381 aaaccgttcc caataaaatg aaaatccgcc gccgtatccg gaaaattcgg aaatgccgca
    25441 tcgtggcatc aaaaccggag cggaaagaat gtcgtatgcc cgacaaaaaa ggggcatacg
    25501 gctgtgccgt attttttgca ccgtcctccg cccttcggcg ggggcgtgaa aatatagtgg
    25561 attaacaaaa atcaggacaa ggcgacggag ccgcagacag tacaaatagt acggaaccga
    25621 ttcactcggt gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc
    25681 cgtactggtt tttgttaatc cactataccg tacaatccgt cgccgatttt tgcctgtttg
    25741 agactgtttg caatgatgcc cgcctacgcc gcgctcgcct tttccgcctt cacttccgcc
    25801 accctcctgc ccggcacatc cgaagccgcc tttgccctgt tcgtccaccg ctttcccgaa
    25861 cacgcatacg gcgcgttgct gtgcgccggc cttgccaacg gattgggcag tatggtttcc
    25921 tattggatgg ggcgtttgct gccctcccga aaaatgccgt ctgaaaaaac actgaatctg
    25981 atgcggcgtt tcggtatttg gctgcttgcg tttgcctggc tgcccgtcgt cggcgacgca
    26041 ctgccgcttg ccgccggctg gctgcggttg aatccgtgga caagcgggct gatgctggtt
    26101 atcggcaaaa cggcgcgtta cgccttcatt ctgtggggaa tgcaatatta cgccgcctga
    26161 accgtgcgtt ataatgccga ccgcacaaac cgactgcaca aaaaaatgaa cagacacatc
    26221 cgccaagaaa ttttcgaacg cttccgcgcc gccaaccccc atccgaccac cgagctgaat
    26281 ttcaactccc ctttcgagct tttaatcgcc gttctgcttt cggcgcaggc gaccgatgtc
    26341 ggcgtaaaca aggcgacggc gaagctgttt ccggttgccg atacgccgca ggcaatgctg
    26401 gatttgggct tggacggcgt gatggaatac acgaaaacca tcgggttgta taaaaccaag
    26461 tccaagcaca tcatgcagac ctgccgcatc ctgctggaaa aatacaaggg cgaagtgccg
    26521 gaagaccgcg aggctttgga atcgttgccg ggcgtggggc gcaaaacggc aaacgtggta
    26581 ttgaacacag cgttcggaca ccccgttatg gcggtcgata cgcatatttt ccgcgtgtcc
    26641 aaccgaacca aaatcgcacc cgggaaagat gtgcgcgaag tcgaagacaa actgatgcgc
    26701 ttcattccta aagaatttct gatggacgcg caccactggc tgattttgca cggacgctac
    26761 acctgcaagg cactcaaacc gcaatgccaa acctgcatca tcaacgattt gtgcgaatat
    26821 cccgccaaag cctgactgcc gccgcccttc aaaatgaaac gcttgaaaat tcggacggac
    26881 gttgtagaat ttcaacctat gccgtctgaa gccgacaaaa tgcgcttcag acggcattaa
    26941 agccggtacc ggcatccgac aaaggacaaa acgtgttcaa aaaataccaa tacctcgctt
    27001 tggcagcact gtgtgccgcc tcgctggcag gctgcgacaa agccggcagc tttttcggtg
    27061 cggacaaaaa agaagcatcc ttcgtagaac gcatcaaaca caccaaagac gacggcagcg
    27121 tcagtatgct gctgcccgac tttgtccaac tggttcaaag cgaaggcccg gcagtcgtca
    27181 atattcaggc agcccccgcc ccgcgcaccc aaaacggcag cagcaatgcc gaaaccgatt
    27241 ccgacccgct tgccgacagc gacccgttct acgaattttt caaacgcctc gtcccgaaca
    27301 tgcccgaaat cccccaagaa gaagcagatg acggcggatt gaacttcggt tcgggcttca
    27361 tcatcagcaa agacggctat attctgacca atacgcacgt cgttaccggc atgggcagta
    27421 tcaaagtcct gctcaacgac aagcgcgaat ataccgccaa actcatcggt tcggatgtcc
    27481 aatccgatgt cgcccttctg aaaatcgacg caacggaaga gctgcccgtc gtcaaaatcg
    27541 gcaatcccaa agatttgaaa ccgggcgaat gggtcgccgc catcggcgcg cccttcggct
    27601 tcgacaacag cgtgaccgcc ggcatcgtgt ccgccaaagg cagaagcctg cccaacgaaa
    27661 gctacacacc cttcatccaa accgacgttg ccatcaatcc gggcaactcc ggcggtccgc
    27721 tgttcaacct gaaaggacag gtcgtcggca tcaactcgca aatatacagc cgcagcggcg
    27781 gatttatggg catctccttt gccatcccca tcgacgttgc catgaatgtt gccgaacagc
    27841 tgaaaaacac cggcaaagtc caacgcgggc aactgggcgt gattattcaa gaagtatcct
    27901 acggtttggc acaatcgttc ggtttggaca aagccggcgg cgcactgatt gccaaaatcc
    27961 tgcccggcag ccccgcagaa cgtgccggcc tgcaggcggg cgacatcgtc ctcagcctcg
    28021 acggcggaga aatacgttct tccggcgacc ttcccgttat ggtcggcgcc attacgccgg
    28081 gaaaagaagt cagcctcggc gtatggcgca aaggcgaaga aatcacaatc aaagtcaagc
    28141 tgggcaacgc cgccgagcat atcggcgcat catccaaaac agatgaagcc ccctacaccg
    28201 aacagcaatc cggtacgttc tcggtcgaat ccgcaggcat tacccttcag acacataccg
    28261 acagcagcgg cggacacctc gtcgtcgtac gggtttccga cgcggcagaa cgcgcaggct
    28321 tgaggcgcgg cgacgaaatt cttgccgtcg ggcaagtccc cgtcaatgac gaagccggtt
    28381 tccgcaaagc tatggacaag gcaggcaaaa acgtccccct gctgatcatg cgccgtggca
    28441 acacgctgtt tatcgcatta aacctgcaat aacacacata atttaaccaa aatatttttt
    28501 aaatgataaa atgcccgtca ataccgatta acgggcattt tacaaaaatc agttggttga
    28561 ttttaaacaa atatttttta cgacaaaaaa tatttccgcc cattttgcca acaaaaccca
    28621 aaccggaggc ggacagatac gctcccatcc ggacgcttaa cacccccata accatgcagc
    28681 tgatcgacta ttcacattca tttttctcgg ttgtgccacc ctttttggca ctggcacttg
    28741 ccgtcattac ccgccgcgta ctgctgtctt taggcatcgg tattctggtc ggcgttgcct
    28801 ttttggtcgg cggcaacccc gtcgacggtc tgacacacct gaaagacatg gtcgtcggct
    28861 tggcttggtc agacggcgat tggtcgctgg gcaaaccaaa aatcttggtt ttcctgatac
    28921 ttttgggtat ttttacttcc ctgctgacct actccggcag caatcaggcg tttgccgact
    28981 gggcaaaacg gcacattaaa aaccggcgcg gcgcgaaaat gctgaccgcc tgcctcgtgt
    29041 tcgtaacctt tatcgacgac tatttccaca gtctcgccgt cggtgcgatt gcccgccccg
    29101 ttaccgacaa gtttaaagtt tcccgcgcca aactcgccta catcctcgac tccactgccg
    29161 cgcctatgtg cgtgctgatg cccgtttcaa gctggggcgc gtcgattatc gccacgcttg
    29221 ccggactgct cgttacctac aaaatcaccg aatacacgcc gatggggacg tttgtcgcca
    29281 tgagcctgat gaactattac gcactgtttg ccctgattat ggtgttcgtc gtcgcatggt
    29341 tctccttcga catcggctcg atggcacgtt tcgaacaagc cgcgttgaac gaagcccacg
    29401 atgaaactgc cgtttcagac ggcagctggg gcagggttta cgcattgatt attcccgttt
    29461 tggccttaat cgcctcaacg gtttccgcca tgatctacac cggtgcacag gcaagcgaaa
    29521 ccttcagcat tttgggggca tttgaaaata ccgacgtgaa cacttcgctg gtattcggcg
    29581 gcacttgcgg cgtccttgcc gtcgttctct gcacgctcgg cacgattaaa accgccgact
    29641 atcccaaagc cgtttggcag ggtgcgaaat ccatgttcgg cgcaatcgcc attttaatcc
    29701 tcgcttggct catcagtatg gttgtcggcg aaatgcacac cggcgattac ctctccacac
    29761 tggttgcggg caacatccat cccggcttcc tgcccgtcat cctcttcctg ctcgccagcg
    29821 tgatggcgtt tgccacaggc acaagctggg ggacgttcgg cattatgctg ccgattgccg
    29881 ccgccatggc ggtcaaagtc gaacccgcgc tgattatccc gtgtatgtcc gcagtaatgg
    29941 cgggggcggt atgcggcgac cactgttcgc ccatctccga cacgaccatc ctgtcgtcca
    30001 caggcgcgcg ctgcaaccac atcgaccacg ttacctcgca actgccttac gccttaaccg
    30061 ttgccgccgc cgccgcatcg ggctacctcg cattgggtct gacaaaatcc gcgctgttgg
    30121 gctttggcac gacaggcatt gtattggcgg tgctgatttt tctgttgaaa gataaaaaac
    30181 gcgccaacgc ctgacccctt tccctgttca gacggcatat gccgtctgaa gctttttgga
    30241 gcaaaccgca atgaccgtcc cccatattcc gcgcggcccc gtcatggcag acattgccgc
    30301 cttccgcctg accgaagagg aaaagcaacg cctgctcgat cccgccgtag gcggcatcat
    30361 cctcttccgc cgcaacttcc aaaacatcga acaactcaaa acactcaccg ccgaaatcaa
    30421 agccctgcgc acacccgaac tcattatcgc cgtcgatcac gaaggcggca gggtgcaacg
    30481 tttcatcgaa ggcttcaccc gcctgcccgc aatgaacgtt ttagggcaaa tttgggacaa
    30541 agacggcgcg tccgccgccg aaaccgccgc cggacaagtc ggccgggtgt tggcaaccga
    30601 gctttccgcc tgcggcatag acctctcctt cacccccgtt ctcgacttgg actggggaca
    30661 atgcgccgtc atcggcaacc gcagtttcca ccacaatccc gaagcggtcg cccacctcgc
    30721 cctcgccctt caaaaaggtt tggcaaaagg cggtatgaaa tcctgcggca aacattttcc
    30781 cggacacgga tttgtcgaag gagacagcca tctggtcttg ccggaagaca ggcgcagcct
    30841 gtccgaactc gaagccgccg acctcgcccc cttccacatt atgagccgcg aaggtatggc
    30901 ggcggttatg cccgcccacg tcgtttatcc acaagtggac acaaagcccg cagggttttc
    30961 cgaaatctgg ctcaaacaaa ttttgcgccg cgatatcgga ttcaaaggcg tgattttctc
    31021 ggacgatttg actatggaag gcgcgtgcgg ggcaggcggc atcaaagaac gcgcccgcat
    31081 ttcctttgag gcaggttgcg acatcgtcct cgtctgcaac cgtcccgacc ttgcggatga
    31141 actgcgcgaa gatttccgca ttcctgacaa tcccgctttg gcgcaacgtt ggcaatatat
    31201 ggccaacacg ctcggcagtg ccgccgcgca agccgttata cagacggcgg atttccaagc
    31261 ggcgcaagcc tttgttgccg gccttgcctc gccgcaagac acggcgggcg gcgtaaaagt
    31321 cggcgaagcc ttttaaatcc gcccgacacg cggcaaaaag gcaaaacggt ataggcacgc
    31381 cgtatttttt gcgctataat cacgccttac ttaattcgga atgtagcgca gcccggtagc
    31441 gcacttcgtt cgggacgaag gggtcggagg ttcgaatcct ctcattccga ccaaatccaa
    31501 accgcctcgg tatccattgc cgaggcagtt tttccttttg acgtcaacag aaaaatgccg
    31561 tctgaaacag aatgagacct ttgcaataac ataggttact aaaattttat gctcaatctc
    31621 attttcaaaa tgcaaaactt ttctgatttt tcctactttt tgctcaatat taggaaagtt
    31681 ttaggcaatt gaaaattttt tggcacattt ttatgcgtca aatttcgtta acagactatt
    31741 tttgcaaagg tctcagaatg ctgtttcaga cggcatttcg ttttccctaa ccctgccatt
    31801 tgcgcgcact ggcacagagt atcggcaagg ttcaaaagac catcggaatt taggaagccg
    31861 ggcttgtacc caacggcggc atcttattca gtaaggctcc gcctcaatca ggcagcggaa
    31921 atgtgggcag tttcacgcgg aagcggcaca cttttcccat atgtcaaaca tcggtaaaac
    31981 cgtacgccgt ccctttgagt tacactgctt aaactactct attcccgccc atttgtgcgt
    32041 ctgcacgctc aactgccaat gcaccggcgc gtcggggcga ctgtttaaaa taccgatttg
    32101 gcggatggtg tcgtagatgt tcatcgcacc gtcttgttcg cagggcgaaa ggtagtaatg
    32161 atgcgcgcgg attttgcgtt ccatattttc acagaacgca acgacatcgc cgtcggcaac
    32221 aatccgcact tcgtcggctg tttcgataca gcttttttcg tatttggcgg catagcaggc
    32281 tttggggctg gtggcgacat agtcgatttg cggcggcgcg ggattgagtc cgttggtttc
    32341 gatacagagg aaataacctt ccgccttgag catgtccagc agcatatcga gatgcggctg
    32401 gatggtcggt tcgccgccgg tgatgatgat gttgcgggcg gcataggctt tcagacggcc
    32461 taagatgtcg gacaggctca tcatgccgaa tgtcaaataa tcggtatcac accagccgca
    32521 ggcgaggttg catttgccca agcggacgaa aacggcgggc atgcccgtgt tccagccttc
    32581 gccttgcagg ctttcaaaga tttcgacgat gcggtattgg ggattttcgg gggcgacgct
    32641 gatttttttc ataccgccaa caccgccgcc gctgccaaaa atgcgggcaa accttgtttg
    32701 acgagtatgc ctttgttgcc ggaagagaac gcgccccacg cggcggcaat caggacgaag
    32761 ccgagaaaca gtaccgttgc gccgtacacc gcgttatccg gagcggcaaa ccgcgaccac
    32821 accagcccga tgccgagaaa gccgttatac agcccttggt tgccgaacag ggtctgcacg
    32881 cgattttgtt ccataaattc ataaggcagc ttgaacgttt ccgccgcttt ttcgctggga
    32941 atctgcgtca tttcaagcca ggcgatgtag aaatgttcga cggcgacgaa gaggactagg
    33001 gggatgaaga ggagtttcat ggatattcct tttaatgggt gtatttgata tggaaggtcg
    33061 tctgaaaaaa cggggaatcc gtacaggaga aaaaaccgtt ttacggtaaa ttcaaataag
    33121 gacgctctaa accgtccttt ccgcgcaggc gggaatccag agatttggaa ttgctgcaat
    33181 ttttaaatat tcctgataaa tcaaggtctg gattcccgcc tgcgcgggaa tgacgcggtt
    33241 gggcatgtct tattggaatt tactatatcg atacgttcaa gatattccct actccccttc
    33301 atactccgca cacgatgtcg gcgtttccca caatttcacg cggcacacgt tcagccccgc
    33361 gtttttcaga cggtcgtaca tttcgaccgc catattttcg gcagtggtgc ggcagggcag
    33421 gcgcagggtt ttcatgttcc agccctccaa aagcgcggcg atttggcatt cgcggctgtt
    33481 gccgccgtgg tagatgaagg cgtggtcgaa ggggtcggta atgtgttgtt tgacaatggc
    33541 tttcaagtcg gtaaagtcca tcaccatacc gtctttcgcg ccgcctttga taatgccgtc
    33601 tgaaacggtg atttcgagtt tgtaggtatg cccgtgcagg ttttggcatt tgccgtcatg
    33661 cccgtcgagc atatgcgagg agtcgaaggt gaagattttg gtgattttca tgggttctca
    33721 aatgggcaaa tagccctgaa agccgatttc ggcgggtttg ccgtgcggat tcggggtcag
    33781 gcggatatgg aaattgccga tgcggtcgag cgcaagcagg ttgtccacgt cttttaagtt
    33841 gtctttatgc tgctgaacat agctgtcggt caacagggtt tgcagggctt tttgtttggc
    33901 ttcggcaggc gtggcggcgg cgaacaaccc gaaagcgtgc gcttcggcga gctgccctgc
    33961 gcgatagccg ccgacattgg caaaatacag gcgcggggcg ttttcagacg gcattttcgt
    34021 tttgggcgtt tcggatacgg cgatgtcgta accgtccgcc cattcgacaa cctgccagcc
    34081 gtcgatgtgg attttgtccg catcgccgaa ccacgcggct ttgagtgcgg ggacggcttc
    34141 acggtagtcg tcgcacacgg caaattggat gtcgtgcact tcgatattcg acctgccggc
    34201 attgccgccg aggtaaaaca tatggagctt gggcatatcg tattccttat ggtttgcgcg
    34261 tgttcagacg gcctttttac tttccagata ttccgccagc ccgcgttcgc gcaagataca
    34321 gctcgggcat tcgcggcagc cgccgacgat gccgttatag caggtgtggg tttgctcgcg
    34381 gatatagtcc agcgcgccca tttcgtccgc caacgcccaa gtttgcgcct tggtcagata
    34441 catcagcggc gtgtggattt gaaaatcata gtccatcgcc agattaaggg taacgttcat
    34501 ggatttgaca aacacgtcac ggcagtcggg atagccggag aagtcggttt cgcacacgcc
    34561 cgcgatgatg tgccgtatcc cctgcccttt ggcgtaaatc gcggcataga gcaggaaaag
    34621 cgcgttgcgg ccgtctacaa aggtattcgg aacgccgttt tcggcagttt cgatggcggc
    34681 ggtgtcgtcc atcagggcat tgtgcgtaat ctgccgcatc aggctcaagt cgagtacggt
    34741 ttgtttgacg cccaaatcct gcgcaatcca gcgggcacgt tccagctcga cggcatggcg
    34801 ttgcccgtat tggaaagtaa tggcttggac gttttcgcgc ccgtaggttt ggattgcctg
    34861 aatcaggcag gtggtcgaat cctgaccgcc cgaaaagatg accaaggctt tttggtttga
    34921 catatcgaat cctagttttg gtgacgcggg aggttttcga accgcgaagt tggacagtaa
    34981 agacgcggat tatacaggca aacgcgttca gacggcatct gaacgccatg ccgtctgaac
    35041 acgcggctta tgccgacttg atggcgagtt ccgccaagcg tttgctttgt gcgaaggcga
    35101 tattgttttc ttcggcggtc gggacggggc cgctgtcggg aatatctttt tcgacggctt
    35161 cgcagacggc ggacactttg gggacggtgc gcaatacggc ttcgcaacct tcaacgctgt
    35221 cgatgccgcg agcgattcgg cgggcgggat tgcgggtgct gccgttttgg aaatagtaga
    35281 ggacgaggat tttgaggggg atttgggttc atgggcggat tccgttaaaa tggttcggtc
    35341 ttcagacggc ataacagttg ttccgatgcc gtctgaaaca gccgcagttc aggcatttgt
    35401 tcctgacggc aaaattggct ataacatcgg gcatcaatgc ccaacttact gaccgggcaa
    35461 gaaattatga cctttttaca acgtttgcaa ggtttggcag acaataaaat ctgtgcgttt
    35521 gcatggttcg tcgtccgccg ctttgatgaa gaacgcgtac cgcaggcggc ggcaagcatg
    35581 acgtttacga cactgctggc actcgtcccc gtgctgaccg tgatggtggc ggtcgcttcg
    35641 attttccccg tgttcgaccg ctggtcggat tcgttcgtct ccttcgtcaa ccaaaccatt
    35701 gtgccgcagg gcgcggacat ggtgttcgac tatatcaatg cgttccgcga gcaggcgaac
    35761 cggctgacgg caatcggcag cgtgatgctg gtcgttacct cgctgatgct gattcggacg
    35821 atagacaata cgttcaaccg catctggcgg gtcaattccc agcgtccgtg gatgatgcag
    35881 tttctcgtct attgggcttt actgacgttc gggccgctgt ctttgggcgt gggcatttcc
    35941 tttatggtcg gctcggtaca ggatgccgcg cttgcctcag gtgcgccgca gtggtcgggc
    36001 gcgttgcgaa cggcggcgac gctgaccttc atgacgcttt tgctgtgggg gctgtaccgc
    36061 ttcgtgccaa accgcttcgt tcccgcgcgg caggcgtttg tcggggcttt ggcaacagcg
    36121 ttctgtctgg aaaccgcgcg ttccctcttt acttggtata tgggcaattt cgacggctac
    36181 cgctcgattt acggcgcgtt tgccgccgtg ccgttttttc tgttgtggct gaacctgttg
    36241 tggacgctgg tcttgggcgg cgcggtgctg acttcttcac tctcctactg gcagggagaa
    36301 gcgttccgca ggggcttcga ctcgcgcgga cggtttgacg acgtgttgaa aatcctgctg
    36361 cttctggatg cggcgcaaaa agaaggcaaa gccttgcctg ttcaggagtt cagacggcat
    36421 atcaatatgg gctacgacga gttgggcgag cttttggaaa agctggcgcg gcacggctac
    36481 atctattccg gcagacaggg ttgggtgttg aaaacggggg cggattcgat tgagttgaac
    36541 gaactcttca agctcttcgt ttaccgtccg ttgcctgtgg aaagggatca tgtgaaccaa
    36601 gctgtcgatg cggtaatgat gccgtgtttg cagactttga acatgacgct ggcagagttt
    36661 gacgctcagg cgaaaaaaca gcagcaatct tgagtatttt tgaaactgta tttttatcct
    36721 aaagacattt tcctatctgc tgattccacc gtttcctttc tgtgtgccgc cataattttt
    36781 atgatagaca agccattcaa gtttgcattg actgcttttt cattggcaat atccaaatgg
    36841 ctggtcgctt catctaaaaa taggattttc ggttcgcaat ataaggcccg cgccaatacg
    36901 atgcgttgtt tttgtccgcc tgacagtgcg ctgcccatat cgccgattaa ggtttcatag
    36961 cccataggca tggcggagat ttcttcgtga atcatagcaa ggcaggcgca atgttcgatt
    37021 tttcctctat ccggttctgt atcgaaaaaa caaggggctg tactagatta gccctaaatc
    37081 ccacaccaat cccgcaggat tttaagctgt tgagacggtg tgccgaagtt aaatcgaaat
    37141 tcgcattctt tcaagaacag cgggaaagat ttgcaatcga ttccgttgta ttttcgcaag
    37201 acacgtttag tctagagtct gtatattaca tttattttta gggtctgcta gccaatttct
    37261 tgttcccttc attattttat cttctgaaag aaaattattt ttttccatgc tattaatatt
    37321 aatgatatga tttttattta aaataatttt tccatataaa atttccggtt tgtaatagca
    37381 agagtctgaa gcaaatcctt gcccgtcaat ataagaaaaa taaatgttat catcctcctt
    37441 ataatattct aaaaccaatc cggatattat tttatcaaaa ttcccttcat ttccatcttt
    37501 catgactaaa atataattag caggccaacc tttatcgtaa acatattcaa actgatagtt
    37561 cccgaaactc tcgatatccg aactaaaaaa gaagaaaagc aaattaatag ccaatacaaa
    37621 aacaaataag aacaaaaata gcaaaatttt caacttagtt aacaatattt acctctcctt
    37681 taaattcaat cctgaaaggt accccttacc ctgggctacc aattatagtt tccatatttc
    37741 taaatattgt ttttacatta cttttttctc cccccaaagg aatgcatttt aaaatcatgc
    37801 ttttcaggtg ctaatcgata cttaccatta acatctttaa tcacagatat atttccatgt
    37861 atagcccaac gtgaaaaatc tgagtattat atacagttat accttactga aacaaagcta
    37921 gctgtacata tctgtaccat atttttacta aaaacaaaaa atatttataa ttattaagga
    37981 taagataaaa acccccaacg ccagattaat tcttaataat ttcaaataaa aaatatttga
    38041 gattttttat tttccaaata atatattgaa atatttaaag ttaaggtagc ttgatttaat
    38101 tagaaaatca aaaaaaccat taattaattt ttcttcttta aatattttat atgatattat
    38161 aaaattaaat aacattgcga aaacatatga aatcatcaaa ataccaaata tatagtggga
    38221 atgcattatt atttagctcc ttcttttggc tttgacaaat tgacagtcca ttgatatcca
    38281 atgcttgcat ttgcaattgc tgacgcacct gcccctgttt tccctgttag tatcaatggt
    38341 tcttttcttg caagatgtgc acctgcacaa ccacctaata ctaaaccacc acctattgta
    38401 aatccttcct gttgtatctc taatgataac attttcttct cgtgccgccc ttaatacaga
    38461 ttgtggaatt ggattgacct gagcaggaac ttctaaaata agttttcctg taagttttct
    38521 tcctcttagc ggagcaggtg tacttggaac attggaaatg acaaagtgag tgcgcaaagc
    38581 cacctgaagg cgatgtgttt gaacctgttg aaagcggcca acaggctaaa tgcgcccgct
    38641 gccgcctaaa aggcagcccg gatgcctgat tatcgggtgt ccgaggagga ttaagggggt
    38701 atttgggtaa aattaggagg tatttggtac gaaaacagcc gaaatcctgt gtttgggttt
    38761 cggatgtcgg ggaagggctt ttttgcaaag gtctcaactc atgttattgc aaagatctca
    38821 tgccttgttt tgccagcagg ttgagttctt ctccggcaat cagcgtggta aagccccgcc
    38881 acaacacaga tctatgtact gtggaagggc ttcaatttat agctttcgaa aatctttcag
    38941 ctttaagacg gcctaaagtt gtaatctata aaacaggagc tcagagtttt acggctcact
    39001 ctcgcttcgc gttaccaaat accagcttat cggttttgaa cgtatagtaa tctatccaat
    39061 cctgcagata ttcatacggg taagacaagt catactgttt cataaaatcg aagatgtctt
    39121 gatattcgtc tgccggcagc ggatcgtaaa caatcaaatc cctcggtgag atttcttgat
    39181 ctatgcccaa atcgaaaccg tgcatggatg cgattttggc gtaggtaacg atttgcggct
    39241 gcaggtagaa atcgaaatag gacaatgttt ctttggcagc catacgcgcg gtttttttat
    39301 cgaaaagcgg ctctaaggcg gctttcattg cctcggcatc ctgttcggcg aaagcgagga
    39361 agaagcggta atcgtacaac cgcttttgca gccatttgct aggggtggga tgcgccaaga
    39421 ctttctcgct acgttgtttc aaccgatcaa gctgcttacc ctccaccatc agcagagtat
    39481 tgtaaatcat atgccggttg aggtcgtagc ggtttataaa ggcttctgta tcgttggcga
    39541 tgttgtcgat attgcgcacc agaaactcac gcagctgtgg gctgtcgctc atcagcatca
    39601 gaaacatcgg attttgaatg ttgagcatgt cacagggaaa gaagaagggt tcagggtctg
    39661 tagaatttac actcagcaaa acccccagct ttcctgcaac ataagcatat tgcttgaatt
    39721 tcttaagatc atgttcaaat agataggcat gggaagctgc agctctaacg tccatgaaaa
    39781 tatgttgcat agctgcaagc ggaacgcctt ttctttcatc tacataatct atagagtcaa
    39841 ggcaaccgct attgaaatta gcagtattgc ctatgattac attagtaata tgctcatacc
    39901 atttttgggt ggtcatcata ttgtgcccca ttgttatctc cttatattgg ttttagaagg
    39961 aactttgaca ggaagaataa cggccttacc tgtttgacga tcaacgcctg ctattgctgt
    40021 ttttaatgtg ccgttcttat ttgctttgaa gacagcagct ttagcgggac taccatcggg
    40081 taattgatct aaaacttgtc taatccaatc ctcactcatt tgcgtatatc cacccgcacc
    40141 attcggattc agctgtaccg taccgttcct aatctgctta ctttctacaa tcaaaacgac
    40201 actaccatcg gcagcctgcc atacatgatc aaaaccgtta tttccgccgt atttgccgcc
    40261 cgaaagcact ctgaagccat tttgtttagc taaagagtca aataactgct cggtcatttt
    40321 acctgtcgtg tcccttcctt cgccaatagc tttcagcaat tgtttgtcag ttgctgcagt
    40381 aacccttaat tcagtttgac cgattttgat tttggttttg ctgacatagc cctgtgcagt
    40441 ggcaacggtt gctgccttgt ccaaggcttt tttcatgcct tgtaaatttt ttgccgcttt
    40501 cgctacttta taggcctgta tcgattcacc gatacccgga accacaccca gcaaagcaaa
    40561 caggtgatcg gcagcggtct gtgcttgtac aaaactctgt atatcaccga taattggaat
    40621 aaatcctgtc tgaaaaagca aaacttctcg atattcttca ggattttcac gtatccattt
    40681 ttgttgttcc ttttgaaatt catcacactt tgcaccaacg catctaggat acaaagtatt
    40741 attctccacc gctatctgtg ccgcattcgc cgctgtattc acatccccgc ctacaacggc
    40801 agacgcagtg ccggcaacca gttttgaata ggcagtaatc ttcgctttag ctttttcgat
    40861 ttcggtcgca ctcatacgac tgaaatcagt attcttaacc aaagcctcac caacaatctc
    40921 accgactgca gcgcctatcg caccatcctg acacttgccc ttattcgccg ccgctgccgc
    40981 acagcccgct atggcatggg caatcttgtg ggtggtatat cgctgccagc cctctctgtt
    41041 gataatcttg cgtatgatta cgccgtttga tggctaatcg tacctacgca ggcttaaaat
    41101 ttccatcaat ccatatcatt tgagttttca tcttcataat atggatgatc aaacaaatct
    41161 ccttcccaaa tacattcact cgttatacct tgggattcca tgaaaggaac ataagaataa
    41221 aaatcttttt catattctcc ataaaatata ttcatacctt tgagccatac tgcatcgcat
    41281 tcatcacaca gaataatttc atcatgaaga tatttaggtt tcgaatggta gagatagcct
    41341 tgttgacaag caggacacat tttcattctc ttattcatga ttattccata taatcaattt
    41401 atttttgtgg gtaagcaata gtgatttgat tgctgccttt agtctgtaca acaatacgta
    41461 tatagcaatc tgataatttc ctggttttta tcagctttat cctcagattc caaacccaat
    41521 aataaaagcc gtttgaaatt tttttcagac ggccttaaaa cttaattaaa taatgtccgg
    41581 atttaacacc aaatcatatt caccaaactt tggaaataga attaccttat ctttttggga
    41641 atataattct gttttttcat atcgctcatt attaataaaa tcttgtttca aaacatagca
    41701 atcatccgta ttttcattga agaaataagt atatccttct gctaagtcat gagattccaa
    41761 agttatataa tctctccata cagccataga aagaaaataa taattcgaat tggaatattt
    41821 ttgaaattga tgggttaaat aatcccttcc aaatttatcg aaaaaattta ccgaaaaaaa
    41881 acctacttcc tctcgcaagg taattacata ttcaggttgt gtcgccgaac ctattacggc
    41941 agtatataac tcccctttaa gatgtttttc ctcagcttgt ttctcagaaa aaggcttatg
    42001 aagtctctta taaccataag accactgctc acaataaaaa atatctttct tcatttgtaa
    42061 atccttcctg ttgtatctct aatgataaca ttttcttctt gtgccgccct taatacagat
    42121 tgtggaattg gattgacctg agcaggaact tctaaaataa gttttccttc aagctttctt
    42181 cctcttatcg tagtaggtgt acttggaaca ttggaaatga cagtaccagg gctatatttt
    42241 ctaacagcct ccttgatata accaatcccc gtactttctt ggatttgaga aaattgggta
    42301 aattttcttg aaataatttc ccttgtcgca ggatcatacg aatctaccct aaaatacccc
    42361 gtcgccgatt taggattggc tacataaagc tcattataag ggtattttga tgacatgata
    42421 cggttaaatt cattgccgtt gtttatcctg attctataaa ttggttcaac agcaaagcct
    42481 ctggattccc ttaattgatt ataatattgc ctgtatgttt gtacatcatg tcttgtccac
    42541 ggctctccag gagtcctcag aatagcaatc ccgttaaatt tcggatccag ccttcggatt
    42601 tgattggtaa tggcctggat ttcaagaatg gcatattcat gttccaattc ttgtcgcgga
    42661 gtccaacgta tatttaccct cctgcgagct aaaagactat tattctccac tgccacagta
    42721 gccgcattcg ccgccgtatt cacatcccct ttaaccaatg ccactgcgct gcctgcgata
    42781 atctgcgagt aggctatgac tttttggcgt tcttggggtg acagtttgcc tacatcgcgt
    42841 ccgtccaaca gggtttctcc caccatctcg ccgactgccg cgccgattgc gccgtcccga
    42901 catttgcctt tatttgctac cgccgatgca cagcctgcta cggcatgggc tatcttgtgg
    42961 gcaatgtagt cttcgctgag attaaatttg attttgctcg ctacttctcc gtgtacggta
    43021 ctgactatcg cacccagtgc ggcatcgccc aagttgtctt tcaggctgcc gccgttgatg
    43081 gcggtatgga cacttgcggc agcggtgctg ttgatcaggt tagcggtcag tttgcctgct
    43141 gcgggactgt gaaaatgttt gctgacggct tcggctgctt gggtgttcag cccgcttatg
    43201 ccctgcagta cgcctgcggt tacggcggcg gtggcggcct gtctgacggt gctgcttttg
    43261 cccagttctt tcagggtatg gtttatgtct cctttgttgt tgatgaggga aactgcggct
    43321 tggctggcga gactggccag tgcggctttg cctgcggctg tggtgatagc tgcagctgat
    43381 gtgcctgctg ctactccggt tgtggctgcc gttcctgctg ctactccagt tccggctgcc
    43441 gttcctgctg cggctcctcc cgctcctgcg cctgccgcgc ccgccgttcc ggcggctgcc
    43501 ggggcggaca gtgcaccgta ggtcaatacg gttacgacga taacgacgac agctgctgct
    43561 gcgggtgtca tgccctcctg tttgtagtcc catttatcgt aagcaagctg cacctgattc
    43621 cagttgatgt ttttcgcaac ttgaagttgt ttcaaataag catactcggg ctgcttggtg
    43681 agggtttcga tttgggtttt cagattgcct ttcggaatgt cgacaatgta accgccgggt
    43741 gcggacagta cgggcgcaac gggaccggtg aaactcggca attgcaaggt ttcgatgtta
    43801 ctgccccgtc ctgcctgttt ttgccataga gtagatttgc tgctgctcac ggtttctgtg
    43861 tggatgctgc ttttgat
//
LOCUS       DAWFKN010000007        42538 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFKN010000007 DAWFKN010000000
VERSION     DAWFKN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.007"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WGR46_000361"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WGR46_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0855373.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2480..2570)
                     /locus_tag="WGR46_000362"
     tRNA            complement(2480..2570)
                     /locus_tag="WGR46_000362"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Ser,seq:gga)
     gene            complement(2609..2695)
                     /locus_tag="WGR46_000363"
     tRNA            complement(2609..2695)
                     /locus_tag="WGR46_000363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2659..2661),aa:Leu,seq:cag)
     gene            complement(2731..2817)
                     /locus_tag="WGR46_000364"
     tRNA            complement(2731..2817)
                     /locus_tag="WGR46_000364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2781..2783),aa:Leu,seq:cag)
     gene            2906..5281
                     /gene="rnr"
                     /locus_tag="WGR46_000365"
     CDS             2906..5281
                     /gene="rnr"
                     /locus_tag="WGR46_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0855374.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERRMRGIMHGDIVTVRPAGMDRRGRREAVVLDIVERAQ
                     SKVVGRFYMDRGVAVLEAEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCSADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGR46_000366"
     CDS             complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGR46_000366"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0855375.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVAGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGR46_000367"
     CDS             complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGR46_000367"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0855376.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLNNPKIQSSLKQTLLEQLS"
     gene            complement(9829..10149)
                     /locus_tag="WGR46_000368"
     CDS             complement(9829..10149)
                     /locus_tag="WGR46_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0855377.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGR46_000369"
     CDS             complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGR46_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0855378.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGR46_000370"
     CDS             complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGR46_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0855379.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGR46_000371"
     CDS             complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGR46_000371"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0855380.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGR46_000372"
     CDS             complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGR46_000372"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0855381.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGTEMTQIADFVQPCPTPDEVAAKLTAHLATQFTPKADNNE"
     gene            complement(13503..13778)
                     /locus_tag="WGR46_000373"
     CDS             complement(13503..13778)
                     /locus_tag="WGR46_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0855382.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            13963..15033
                     /locus_tag="WGR46_000374"
     CDS             13963..15033
                     /locus_tag="WGR46_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0855383.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFTGSFYRGR"
     gene            complement(15140..16087)
                     /locus_tag="WGR46_000375"
     CDS             complement(15140..16087)
                     /locus_tag="WGR46_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0855384.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(16102..16509)
                     /locus_tag="WGR46_000376"
     CDS             complement(16102..16509)
                     /locus_tag="WGR46_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0855385.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIIYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            16590..17249
                     /gene="ung"
                     /locus_tag="WGR46_000377"
     CDS             16590..17249
                     /gene="ung"
                     /locus_tag="WGR46_000377"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0855386.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            17246..17455
                     /locus_tag="WGR46_000378"
     CDS             17246..17455
                     /locus_tag="WGR46_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0855387.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            17352..17999
                     /locus_tag="WGR46_000379"
     CDS             17352..17999
                     /locus_tag="WGR46_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0855388.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            18072..18545
                     /locus_tag="WGR46_000380"
     CDS             18072..18545
                     /locus_tag="WGR46_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0855389.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTSTNLVTAVWCI"
     gene            18533..18847
                     /locus_tag="WGR46_000381"
     CDS             18533..18847
                     /locus_tag="WGR46_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ0855390.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            18889..19833
                     /locus_tag="WGR46_000382"
     CDS             18889..19833
                     /locus_tag="WGR46_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0855391.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            19975..20109
                     /locus_tag="WGR46_000383"
     CDS             19975..20109
                     /locus_tag="WGR46_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0855392.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(20399..20914)
                     /locus_tag="WGR46_000384"
     CDS             complement(20399..20914)
                     /locus_tag="WGR46_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855393.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(21380..23299)
                     /locus_tag="WGR46_000385"
     CDS             complement(21380..23299)
                     /locus_tag="WGR46_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0855394.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(23376..23771)
                     /locus_tag="WGR46_000386"
     CDS             complement(23376..23771)
                     /locus_tag="WGR46_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0855395.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            23944..25251
                     /locus_tag="WGR46_000387"
     CDS             23944..25251
                     /locus_tag="WGR46_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0855396.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            25244..25678
                     /locus_tag="WGR46_000388"
     CDS             25244..25678
                     /locus_tag="WGR46_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855397.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKSE"
     gene            complement(25737..26006)
                     /locus_tag="WGR46_000389"
     CDS             complement(25737..26006)
                     /locus_tag="WGR46_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0855398.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGR46_000390"
     CDS             complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGR46_000390"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0855399.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNFKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAVAESASKPKPRSRATKH"
     gene            28735..28926
                     /locus_tag="WGR46_000391"
     CDS             28735..28926
                     /locus_tag="WGR46_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0855400.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            28901..30004
                     /locus_tag="WGR46_000392"
     CDS             28901..30004
                     /locus_tag="WGR46_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0855401.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGR46_000393"
     CDS             complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGR46_000393"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0855402.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGKDENLPNIALAAPTGKAAAHMARALHRAINGFDALEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGR46_000394"
     CDS             complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGR46_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0855403.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(32577..33824)
                     /locus_tag="WGR46_000395"
     CDS             complement(32577..33824)
                     /locus_tag="WGR46_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0855404.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            34030..34308
                     /locus_tag="WGR46_000396"
     CDS             34030..34308
                     /locus_tag="WGR46_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855405.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGR46_000397"
     CDS             complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGR46_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0855406.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(35048..36586)
                     /locus_tag="WGR46_000398"
     CDS             complement(35048..36586)
                     /locus_tag="WGR46_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0855407.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(36640..37014)
                     /locus_tag="WGR46_000399"
     CDS             complement(36640..37014)
                     /locus_tag="WGR46_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0855408.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            37164..38792
                     /locus_tag="WGR46_000400"
     CDS             37164..38792
                     /locus_tag="WGR46_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0855409.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            38864..40117
                     /locus_tag="WGR46_000401"
     CDS             38864..40117
                     /locus_tag="WGR46_000401"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0855410.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            40340..40648
                     /locus_tag="WGR46_000402"
     CDS             40340..40648
                     /locus_tag="WGR46_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855411.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            40730..41761
                     /gene="ruvB"
                     /locus_tag="WGR46_000403"
     CDS             40730..41761
                     /gene="ruvB"
                     /locus_tag="WGR46_000403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0855412.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGR46_000404"
     CDS             complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGR46_000404"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0855413.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
BASE COUNT         9706 a        11160 c        11394 g        10278 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gacaagccgt ccgctcgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacga atcaaacccg gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggta tcggcgtaac
      961 catcaccgac aaagacaatc ccaaaggcct accgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagatacc gccgatgccg acgtgttccg cgtatccggg cgcggcgagc tgcacctgac
     1201 cattttgctg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccgtat gaaaacctga ccgtggatgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 tgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 tcgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga agaggatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     2221 atagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     2281 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaaatatacc
     2341 gcaaaacata ccataaaaaa aacggtaacg gatagttgta agcggttgat gacgattata
     2401 gacaataacg ggttttccca atgaaattat tgtttgaata ataaaaaatc ccaaaccgta
     2461 aaagtttggg atttgtattt ggcagagagg aagggattcg aaccctcgat acgctattca
     2521 cgtatacacg ctttccaggc gtgcgactta aaccactcat ccacctctct aatggcggaa
     2581 attatcccaa tcgggataat ttattatttg gtgcccggga gaagactcga acttccacac
     2641 ccatgaggat accagcacct gaagctggcg cgtctaccaa ttccgccacc cgggcaatct
     2701 aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg gagaagactc gaacttccac
     2761 acccgtgagg atactagcac ctgaagctag cgcgtctacc aattccgcca cccgggcttt
     2821 gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa gaagtcgcta ttatatagtt
     2881 cataaagaga atgtcaacag tccaaatgaa taaaaatatt aaatctttaa atttacggga
     2941 aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa catcctttgc ccagtcggga
     3001 atggataatc gaattgttgg agcgcaaagg tgtgccttca aaaatcgaat cgcttgcgcg
     3061 cgagctgtcg attacggaag acgagtatgt ctttttcgaa cgccgtctga aggcgatggc
     3121 gcgggacggt caggttttaa tcaaccgccg gggcgcggtt tgcgcggcgg acaaattgga
     3181 tttggtcaaa tgccgtgtcg aggcgcacaa agacggcttc ggttttgccg tgccgctcac
     3241 gcccgccaaa gacggtgatt ttgttttgta cgaacgccgg atgcgcggca ttatgcacgg
     3301 cgatattgtc actgttcgtc ctgccggcat ggaccgcagg gggcggcggg aagcggtggt
     3361 tttggatatt gtcgaacgcg cgcaaagcaa agtggtcggg cgtttctata tggatcgcgg
     3421 cgtggcggtt ttggaagcgg aagacaagcg tctgaaccaa agcatcgtat tggaaccgga
     3481 cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc gtcggcgaaa ttgaggttta
     3541 tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa gttttgggcg attatgccga
     3601 cagcggcatg gagattgaaa ttgccgtgcg caagcatcat ttgccgcacc aattcagtga
     3661 agcgtgtgcc aaagccgcga aaaaaattcc cgaccatgta cgcaaaagcg atttgaaagg
     3721 ccgcgtcgat ttgcgcgatt tgcctttggt aacgatagac ggcgaaacgg cgcgcgattt
     3781 tgacgatgcc gtgtttgccg agaaaatcgg acgcaattac cgtctggtcg tggcgattgc
     3841 cgatgtcagc cattatgtcc gccccgatga cgctatcgac acggacgctc aggaacgcag
     3901 caccagtgtt tacttcccgc gccgcgtgat tcccatgttg ccggaaaacc tgtccaacgg
     3961 catctgctcg ctcaatccta atgtcgagcg tttgtgtatg gtgtgcgata tggtcatcac
     4021 ttacgcgggc aatattaaag aataccgctt ctaccccgcc gtgatgcgct ctcatgcccg
     4081 cctgacctac aaccaagttt ggaaatggct ttcaggcggc atcgggcatc cgttcaaagc
     4141 ccaaatcgac acgctttaca aactcttcaa aatccttcag aaaaagcgtt tcgaacgcgg
     4201 ggcggtggag tttgacagca tcgaaaccca aatgcttttc gacgacaacg gtaaaattga
     4261 aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg attgaagaat gtatgctggc
     4321 ggcgaatgtt tgctcagcgg attttctatt gaaaaataag catacggctt tgtttcgcaa
     4381 ccatttgggc cccacgcccg aaaaactcgc caccctgcgc gagcagctcg gtctgttggg
     4441 gcttcaactt ggcggcggcg acaacccgtc gccgaaagac tatgccgcgc ttgtcgaaca
     4501 attcaaaggc agacctgatg ccgaattgct gcaagtcatg atgttgcgct ccatgcagca
     4561 ggcggtttac gaaccgcatt gcgacggaca ctttggtctt gcctacgaag catacgccca
     4621 cttcacctcg cccatccgcc gctatcccga cctgaccgta caccgcgcca tcaaagccgt
     4681 gttgaatcag caaacctaca cgccaaaaaa aagctggcag gctttgggcg tgcatacctc
     4741 gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg gaaaactggc tgaaaaccta
     4801 ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa atctccggca tgaccagttt
     4861 tggtatcttt gtaacactgg acggcatcca cattgacggc ttggtgcata tcagcgattt
     4921 gggcgaagac tatttcaact tccgccccga aatcatggca atcgaaggcg aacgcagcgg
     4981 catccgtttc aacatggggg acagggttgc cgtccgggtc gcccgtgccg atttggatga
     5041 tggaaaaatc gattttgtcc tgattgccga ggggagcggc agggggcgga aagttaaatc
     5101 atccgcgtct gccaaaccgg cagggacggc ggggaaaggg aaaccgaaaa ccgccgccga
     5161 gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc gcgtctgccg ccgcagaatc
     5221 ggggaaaaag gcaaagaaac cggttccgat taaggtaaaa aaacggaaag gcaaatcata
     5281 atgctgacgg ggtggcttgg ggagggcggg gcatactttc ccgaagccgc cgcccgattt
     5341 tttgatatgg cggccggcgc atcatttcag gccaatcgaa acgcccgtat tgaaagattg
     5401 cgtttatttc caccgccgtt ttaaaggccg acggtattcg gcagacgggg cgcaaacggc
     5461 gttcagacgg cattttcatt ctttcggcgt gtccgtccga attgctttgc ccgtccgcgc
     5521 aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc cgtctgaaac ccgaaaatca
     5581 ggtttcagac ggcatttcgc cttggaaagg ctgttcaaat cagcggtggt aattcggtgc
     5641 ttctttggta atttgaacgt cgtgaacgtg cgattcgctc atacctgcgg aagtgatttc
     5701 cacaaattcg gctttttcgt gcatttcggc aatattggcg caacccaaat aacccatact
     5761 ggaacgcaaa ccgccggtca gttggtggat gatgttcaca atcggacctt tgtaaggaac
     5821 gcggccttcg atgccttcgg ggacgtattt gtcggtgctg tcggttttgt cttggaagta
     5881 gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc ataccgcgat aggatttgta
     5941 cgagcggcct tggtagagtt cgatttcgcc cggtgcttct tccgtgccgg caaacatacc
     6001 gccgagcatg acgctgtacg cgcctgcggc gagggctttg gcgatgtcgc cggagaagcg
     6061 gatgccgccg tcggcaatca gcggaacgcc cgtgcctttg agggcttcgg caacgttgtg
     6121 aatggcggtc agttgcggca cgcccacgcc tgccacgata cgggtggtgc aaatcgaacc
     6181 cggaccgata ccgactttga cggcatccgc gccggcggca accaaatcca aagcggcttt
     6241 ggcagtggcg atattgccgc cgatgacttg gatgtgcgga taagtttctt tgacccaacg
     6301 cacgcgatcg attacgcctt ggctgtgtcc gtgtgcggta tcgacgacaa ttacgtccgc
     6361 gcctgcttcg accaaggctt tgacgcgttc ttcggtatcg ccgccggtgc cgactgccgc
     6421 accgacgcgc agacggcctt cggagtcttt gttggcattg ggaaattcgg tggtttttaa
     6481 aatgtcttta acggtaatca agcctttaag ctcgtctttt tcgttcaaga ctaaaacgcg
     6541 ctcgacttta tgcgtgtgca tcagttcgcg cgcttcgtct atgcttgtgc cttcggggac
     6601 ggtaaccaag cgttcgcgcg gggtcataat ggcggaaacg ggcaaatcga cgcggttttc
     6661 aaaacgcagg tcgcggttgg ttacgatgcc ggcgactttg ccgttttcaa cgacgggcag
     6721 gccggacatt ttgcgtttgc gctgcgcgcg catttccaag acttcgcgga tgagcgttgt
     6781 cggcgcaacg gttacggggt ctttgaccac gccgctttcg tggcgtttca ctttggaaac
     6841 ggcgcgagcc tgcatctccg gcggcatatt tttatggatg atgccgatgc cgccttcctg
     6901 tgccatcgaa atggcgaggc gcgcttcggt aacggtgtcc atcgcggcgg aaagcagggg
     6961 gaggttgagc gtgatttcgc gggtgagctt ggtttgaagt ttaacgtctc gcggcagcac
     7021 ggttgagtgt gcgggaacca acaaaacatc gtcgaaagta taggcttttt ctacgatacg
     7081 cataatgctc ggtctttcag tttgtgcaag atgcacggca ttatagcacg ttgccggcgg
     7141 cttgacagtt tatattgttt gcaatatccc cgccttcagg cggcatacgg cgcggcggcg
     7201 gaatgccgtc tgaaggcggg cgttatgata attgttccag cagcgtctgc ttcaatgagg
     7261 actggatttt ggggttgttc aaatcggggc tgaaaacggt aaaactgtct tcggcgcgtt
     7321 cgtccaacgt ggagattttg gcgtagcgca ggctgacgtt gtgggcgaaa aagacttccg
     7381 ccatatcggc gagcaggaag gggcggttga cggcggtgat ttcgacggaa taccagtcgg
     7441 gatagtcttc ttcgggggtg atggtgatgc tcggtgcgat cggcatatag cggctgcggc
     7501 ggctgatgcg gcggctgcgg ctttgggttt cggcaacggt gtgtccgtgg ataaagctgt
     7561 tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggggcg tgctgcgagg
     7621 ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgatgaag gcgcgggcgg
     7681 cgaggatgtc gaagccgtgg cggctgaaga tgcggcagag gcgggcgaac aggcgcgggc
     7741 cgttgggcat gaaaaccatg acttgaaagc tgtcgctttt gggcaggatg cggctgcgga
     7801 cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg ccacaggatt tcgcgggact
     7861 ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag ttttttctgc tgtttttcgg
     7921 ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg gcggcggccg aagagggtgt
     7981 gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc atggaagagg ctttccagca
     8041 ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca
     8101 gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaaa gcatcgagta
     8161 cgtcggggtc ttggatgtct tctttttggg cgacggcaga catcagcagg tggttttcga
     8221 ccagccaggc gagcaggtcg ctttcttctc cggtcaggaa gtggtcagcg gcaaattggc
     8281 gcgcgtctgc gatgccttgt atggcatggt cgccgccgcg tcctttggcg atgtcatgga
     8341 aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt
     8401 agggcagctc gtggctgtgc atatccaagg caaggcggcg gacgttgcgg acgacggtga
     8461 ggatgtggtc gtccacggga tagatgtgga acaggtcgtg ttggagcagg ccgatgattt
     8521 tttcccacgc gggcaggtag cggcccaaca cgccgtagag gttgagaaag cgcagggtct
     8581 gggtcagccc gttgccgttg cggaaaaaac cggcgaagcg gcggcggttt tcgggatttt
     8641 ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca ggcgcgcagg gtttgcggtt
     8701 cgagcgcggt aatgtcgttg cgctgctgca tgatttcgac gattttgaaa atgtgttcgg
     8761 gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg gttgttgact tggatgtagt
     8821 cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga aacgcggctt tgcagcatag
     8881 gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca
     8941 gttcttcgct ttggcggcgg aggttcaagc cttcataacc catgctttcg gcgacttgcg
     9001 gctgcaaatc gaacagcagg cggtcttcgg cgcgattggc gtttaaatgc agatggatgc
     9061 ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa
     9121 tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa
     9181 gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc tccaatactg
     9241 cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccagtttt gcctcgacaa
     9301 acgccgccac attgcgctgc gcgttcattt tttccgccag tttatccacc gtttggcggt
     9361 tgccaaacaa aaacctagcc tctaaaaacg ctgtgtcccc cgtaatatca ttgcgcacgc
     9421 tttcacatag ttcatcaacg ctgccgcttt ttacagacgg catcagtttg cagtcccaca
     9481 gggtttgaac aaaccgggca atctgttcct gaatgccgtc tgaaagcggg gcaggggaga
     9541 caaccgccaa atccacatcc gaacagggat acagttcgcc gcgcccgaag ccgcccaccg
     9601 ccatcaggca taacgcgctg ttttgaaaat gttctaccca caatgccgcc agtaaggttt
     9661 cgactgccgc cgtatattcc ctgaaaaata ccgacacgcg gttggctttc aaataatgcg
     9721 cttcggcggc atcgcgctgc cgtttgaagg tttccagtgc tgaagacagg tttgcaggca
     9781 tttttattct ttcgattggc gggaaaaggg aggtggatgg ttcggcggtc aaataccgct
     9841 ttcagacggc atttgtcggg tatcggcgga attggtcatc agcttcaacc gttcctgcgg
     9901 cggcagcagc aactgatacg ccgccacgcc caaagccgcc gccatttttt cgatattcga
     9961 cagggcgatg ttccagcgtt tgcgctcgac tgccgacaca taagtcctgt ccaaaccgca
    10021 ttgccgcgcc aattcttctt gcgaccaacc cttgttcacg cggaaaagcc gcatattgta
    10081 tgccaatacc gcccgcaaat cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt
    10141 gcccatcatt tgcttccaaa taaatggtta aagttaagca tttgctgtta cggattttac
    10201 ttcacataaa agccaaaaaa aaggggtggc ggcagccaca cccaaacaca cacacatcaa
    10261 gaggaaagag gtaacatcag gacagattgg gaaacagcaa acaagcaatc tagatttcat
    10321 atcggtatta tcaaacaaaa accgttatcg agtattgatt caaatcaatt gagatttgat
    10381 tcgcaaaccg gtaaagaaaa acggcatacc cgtttatacg gatatgccgt ctgatgatgc
    10441 aggggactgc tgtatttggc agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag
    10501 gtagttcggc aaaccaattt tgccgattag ttcctgctgg gtttcgagcc agtcgatgtg
    10561 ttcttcgttg gtgtcttttt gtttttccaa caaatcacgg ctgacataat cctgttgcgc
    10621 ttctgccacg gaaattgccg caatcaatgc ctcatgtttt tctttctctt tttccaaatc
    10681 gcaggcgatg atttcttcgg tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt
    10741 cggcagacct tcgaggaaca gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac
    10801 gatggactgt ttgaagaaat gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg
    10861 ggcgtgaagg aaatattggt tgatggttac cagcagcaag cctaagtttt tgtttagctc
    10921 gcggataacc agacggtcgc ctttcatacg gactcctttt attccgaata cggattacag
    10981 ttggctttgg tagtagttgc cttcgccaat taactcgatc aggcgaagct gctgttccag
    11041 ccagtgggcg tggtcttctt cggtatcttt cagttgggca accatcaggt cgcgcgtaac
    11101 atagtcttga gcctcttcgc acagtttgat gccttttttc aacgcatcac gtacttcata
    11161 ttcggtttgc aggtcggctt tgaggcagga aaccacgtcc gtgccgatat tcagttcggc
    11221 gcgcgccatt ttcggcgtgc cgcccagcat caggatgcgg cggatgaagt cttcggcgtg
    11281 tgtggtttct tcttccatct cgtgattgag acgttcaaaa agtttggtgt agccccattc
    11341 ggagtagagg cgggagtgga taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa
    11401 ttcgttcata taatcaacaa cagcttgatt gccttgcata atatctctct ttctgtaact
    11461 tggttttcgg tatgtctgaa cagcatctgt gtcgctattc gggatgcgtg cattgtatgc
    11521 aaaagctgtc ggcatgacaa attttctatt taaaatacaa acaattatca aaagaaatag
    11581 ggcgtattcc ccgatgcctt ccaaatcagt atgctgtttc ttatcggttt ttttgctgct
    11641 aaaactaaga atccatctca tccataaaga acatttacac ttattaacca tagcgaaaag
    11701 caaatactgt acggtttttt atcaaaattt ataatgaatc gttctcatta accgacagat
    11761 tcttttgaga ttatcggatt ttgggaataa attatgccgt ctgaggggct ttcagacggc
    11821 atagacaatt aaagctgatt aaacaacgcg gcagacagca gcgtaggaaa ttatttttta
    11881 cttcggaacg gtattttggg tttaatgatg cccgcaaccg ccgtggcttt cgcgtaaggc
    11941 ttcggcagcc tgttcgtcgg cgtggtagct ggaacgtacc atcgcgccga tggcggcatt
    12001 gctgaagccc agttcgtatg cttctttttc aaagattttg aactgctcgg gcgtaacgta
    12061 gcgcaagaca ggcaagtggc cgtctgaagg ctggagatac tgtccgatgg taatcatctc
    12121 gatattgtgc gcccgcatat cgcgcataat ttcgcgcacg tcttcgtctg tttcgcccaa
    12181 gccgaccatg atgccggatt tggtcgggat gtgcggcatc atttctttat aacgttttaa
    12241 taagtctaaa gaatgttgat aattggcacc gggacgggct tttctgtaca ggctcggatg
    12301 ggtttctagg ttgtggttca tcacgtcggg cggggtttcg gcaaggattt tgagtgcgat
    12361 gtccaagcgt cctcggaagt cggggacgag gatttcgatt ttggtgttcg ggctggtttc
    12421 gcggatggct ttgatgcagt cggcgaaatg ctgtgcgccg ccgtcgcgca ggtcgtcgcg
    12481 gtcgacggag gtgatgacga cgtaacgcag gttcatggct ttgacggatt cggcgaggtt
    12541 tttcggttcg tcggggtcga gcatattggg ccgaccgtgt cccacgtcgc agaacgggca
    12601 gcggcgggtg cagatgtcac ccataatcat gaaggtcgcc gtgcctttgc tgaagcattc
    12661 gccgatgttg gggcaggagg cttcctcgca aacggtgtgc atcttttgtt cgcgcaaaat
    12721 gtctttgatt tcaaagaatt tgcgcgatgg gagtttggcg cgtatccatt cgggcttttt
    12781 cagtttctcc tgaaggggga cgactttgat ggggatgcgg gcggttttgt ccgcgcctct
    12841 gagtttgatg ccgcgtttgg ggtcgtcggt tttgatttca ctcattgttg tctgctttcg
    12901 gtgtgaattg tgttgcaagg tgtgcggtaa gtttggcggc gacttcgtcc ggcgtggggc
    12961 agggttggac aaaatccgcg atttgcgtca tttccgtacc ggcgtagccg caggggttga
    13021 tgtgggtaaa cgggcttaaa tccatattga cgttgagcgc aagcccgtga tagacggagc
    13081 cgtttttgat acgcagcccc agtgaggcga ttttgcgttc tccgacataa acgccggggc
    13141 gtttggggtc tgccgccgct tcgatgccgt attctgccaa tgtggcgatg atgctgtttt
    13201 caagcgcgga aacgatgttt ctaacactgg ttttgcgccg tttgaaatca atcatcgtat
    13261 aaacgaccaa ttgcccgggc ccgtgatagg taatctgccc gccccggtcg atttggacga
    13321 cgggaatgtc gtcgcgaatc agcaggtgtt cgggttttcc cgccagtcct tgtgtgaaca
    13381 cgggcgggtg ttcgacgacc cacagttcgt cttcggtgtc ggcattccgt ccggcattaa
    13441 aggttttcat cgcttcaaaa gtcggcagat attcgaccaa acctttgtgt atgattttca
    13501 tctcaaagta ccactttgac cagttcgtgc gaagtcagcg cacggtagat gttgtccaat
    13561 tgttcttggt tttcaacctt tacctgtacg gtggcgccag tatagttgcc tttgctgctc
    13621 ggacgcgtgg tgatgtggtg cgcctgcgtg tcgggggcgt ggaggcggac ggtgtctaaa
    13681 accgcctgct cgaactcggg atgcaccgcg cccattactt tcaatgggaa ggtgcaggga
    13741 aattcgatga gggatgtttt gtttttttgt tcggtcatga tgtgctgcct tatcgtgtac
    13801 ggtatgccgt ctgaaggcgg gtttgccttt cagacggcat cggatgtgcg ttattttagc
    13861 ctaaaccgcg ataacaggct atcgggaagg cggaggcttt tttgacggcg cggcggttct
    13921 gctatactgg cgcacaatat tatttttaga agggtggttt ttatgtatcg gaggaaaggg
    13981 cggggcatca agccgtggat gggtgccggt gcggcgtttg ccgccttggt ctggctggtt
    14041 ttcgcgctcg gcgatacttt gactccgttt gcggttgcgg cggtgctggc gtatgtattg
    14101 gaccctttgg tcgaatggtt gcagaaaaag ggtttgaacc gtgcatccgc ttcgatgtct
    14161 gtgatggtgt tttccttgat tttgttgttg gcattattgt tgattatcgt ccctatgctg
    14221 gtcgggcagt tcaacaattt ggcatcgcgc ctgccccaat taatcggttt tatgcagaac
    14281 acgctgctgc cgtggttgaa aaatacaatc ggcggatatg tggaaatcga tcaggcatct
    14341 attattgcgt ggcttcaggc gcatacggga gagttgagca acgcgcttaa ggcgtggttt
    14401 cccgttttga tgaggcaggg cggcaatatt gtcagcagta tcggcaacct actgctgctg
    14461 cccttgctgc tttattattt cctgctggat tggcagcggt ggtcgtgcgg catcgccaaa
    14521 ctggttccga ggcgttttgc cggtgcttat acgcgcatta cgggtaattt gaacgaggta
    14581 ttgggcgaat ttttgcgcgg gcagcttctg gtgatgctga ttatgggttt ggtttacggc
    14641 ttggggttgg tgctggtcgg gctggattcg gggtttgcaa tcggtatggt tgccggtatt
    14701 ttggtttttg ttccctattt gggcgcgttt acaggactgc tgctggcaac cgtcgccgcc
    14761 ttgctccagt tcggttcgtg gaacggcatc ctatcggttt gggcggtttt tgccgtagga
    14821 cagtttctcg aaagtttttt cattacgccg aaaatcgtag gagaccgtat cggcctgtcg
    14881 ccgttttggg ttatcttttc gctgatggcg ttcggacagc tgatgggttt cgtcggaatg
    14941 ttggcaggat tgcctttggc cgccgtaacc ttggtcttgc ttcgcgaggg cgtgcagaaa
    15001 tattttaccg gcagttttta ccggggcagg taggcggttc cgaaacatat ttgaagcgga
    15061 atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac gggacttcag
    15121 acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt ttcataccgg
    15181 cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta ttgctttctt
    15241 tggcaagatt gttgaacgcg gcgacgtatt gttccgcaat cttcagattg accgcatccg
    15301 caccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca ttggcttcgg
    15361 caacaaggcg caaggattcc gcttcacctt tggcgcggtt gatgcgggcg attttctcgg
    15421 catttgacgc attgaccgca gcctgagcct cgccttcgga ttgttggatt tcggcttcgc
    15481 gctgaccact ggcaaggttg atttgttcga ttttacgacc ttcggattcg gcgatacggg
    15541 cgcgtttttc gcgttcagca gtaatttgcg cctgcattga gcgaaggatt tcttgcggcg
    15601 gaaccaagtc tttaatctca taacgcaaaa ccttcacacc ccaagctccg gcggcttcat
    15661 cgagggcgga gacgacggtg ctgttgattt cgtcgcgttc ttcaaacgtt ttgtccaatt
    15721 ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc gccataatgt
    15781 agttgctcga accgtatgag gcgagtttgg ggtcggttac ttggaaatag atgatgccgt
    15841 caaccgtcag ctgggtgttg tcgcgcgtga tgcagacctg gctgggtacg tctaaaggga
    15901 tttctttcag cgaatgacgg taggcgacgc ggtcgataaa gggaatcaaa atattcaaac
    15961 cggccgtcaa ggcgcgatgg aaacgtccaa gcctttcgac aacgtggact tcctgctgtg
    16021 ggatgacgac aaaggatttg aagccgaaaa cggcaacggc taccaacaag ataatgaaaa
    16081 attccataat tcctccgagt gttaagggtt tgcgatgata agaaggttgc cttccttgcg
    16141 gacgattagg gcgcgagttc ctggttcaag ctcttcttgc cccgtatttt gagcctgcca
    16201 gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gtgtgtcgga ggatttcgac
    16261 atattgtccg gcatccaaat cctgatatga atccgtttca acttttccca cggcggtttt
    16321 ggcgtgtacg aaccaaatac ccagcgcgga aagcagagca gcggtcaaga cggcggcagg
    16381 cgtgctgccg gtcagtccgt agataatgcc cgaacccgcc aaagccgcgc tgacaaccag
    16441 aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcag caacaaacca
    16501 tacagtcata catttcccca caaagcgcgt cgtttgacaa aatcacgcga tatcagcagt
    16561 atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca ctgggcaacg
    16621 aaaaacggca accgtatttt caggaaattt taaatgcagt caggcaggaa cgtttgtcgg
    16681 ggcaaatcat ctatccgccg gcggaggatg tgttcaacgc attccgcctg acagcgtttg
    16741 accgagtcaa agtcgtcatt ctcggacaag acccgtatca cggggcaggg caggcgcacg
    16801 gtttggcatt ttccgtcagg cagggcgtcc gcataccccc gtctttactc aatatctaca
    16861 aagaattgga aaccgacatc gaaggctttt ccattcccgc gcacggctgc ctgacagcgt
    16921 gggcggagca gggcgtatta cttctgaaca cggttttgac ggtgcgtgca ggacaggcgc
    16981 attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg cagcttgcga
    17041 cacaccgcaa gcatcttgtc tttatgctgt ggggtgggta tgcacaacaa aaaaggaaac
    17101 tgatagacag tcaaaaccat ttgatattga ccgcaccgca tccgtctcct ctgtcggcat
    17161 atcgcggttt tttcggttgc cgccattttt cacaggcaaa cagctatttg agccggcacg
    17221 gtatcgatcc gataaactgg aagctgtgaa tgccgatata gccgttgccg ccggcgtgtt
    17281 aaaatcgcgt ttgatttgta atttccattt attaggcaaa accttatggc aatcaaaaaa
    17341 aaatcagctt tatgcatcac tttgggcggg ctgcgacgag ttgcgcggcg gtatggatgc
    17401 gagcctttat aaagaactat gtgcttacgc tattgttttt aaaatatgtt tctgataagc
    17461 ataagtacgg cggcggcatg attgagctgc acgccggtac gacttttgac gacatcgtca
    17521 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
    17581 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
    17641 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
    17701 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
    17761 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
    17821 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
    17881 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
    17941 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
    18001 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
    18061 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
    18121 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
    18181 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
    18241 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
    18301 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
    18361 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagt ggcggcagcg
    18421 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
    18481 agcaagacat tcacaaaatc atcgacactt ccacaaacct cgttacagcc gtatggtgca
    18541 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
    18601 agaagttgaa gacctgcaaa atctcgccgc ctatctttat ggcggcatac ctgcgcacga
    18661 tatggacgca ttggaagcct attggcaagt tttaggccgt atgaaaaacg agttgtttgc
    18721 cgaacacgat ggccgcttta ccactataca acggaatcga ttgcaaatct ttcccactct
    18781 caacagctta aaatcctgcg ggattggtgt ggaatttagg gctaatctag tacagcccca
    18841 aatttaatcc actataaaat cgaaagcagc caaatcaaag cccatatatt ggcgcacccc
    18901 gattacgccg ccttcaaagc cggacacctg gcaaagtttg ccgcgtggca cactcaaaac
    18961 gaccttgccg ccatccaacc gggcaggctt atccggaaat ggagcgaaag cctgctggac
    19021 gcgttcaaac ccggcagcct gattgaagaa tacgatttct accaaatcct gacggactac
    19081 tgggcggaaa ccctgcaaga cgatgtttat ctcatcgccc aagatggctg gaaggcggtt
    19141 aaaaacctgg ccgaaatcac caaagaaagc gatgaagacg cgaacctgac cgtcgtcttt
    19201 gaggaaaccg aaaccggcaa aaaaggcaaa gccaaaacca agcgcatcag caaaaaatac
    19261 cgcagcgaag tcatcgcccc cgagctggtt gcccgccgct acttttcaga cggcatcgcc
    19321 aagctggaag aaaaacaaag cgagctggaa cgcctaagcc aagaattgga aaaccacata
    19381 gaagaacacg gcggcgaaga gggtgcgctg aacgacgtat tggatgcaaa aggcaaactt
    19441 tccgccaaac ttctgaaaac cgcattggaa gaaagcggca tagaagaagg cgaacgggct
    19501 gttttacaaa ccacccaaac actgatgacg caggaaaaag ccgcgaaaga cgcagtcaaa
    19561 acccaaatcg aagccctgaa ccttgccgta ttcaaacaat tcggccgact ttccgaagcc
    19621 gaaatcaagc agcttgccgt tcaagacaaa tggcttgccg atttacaaag ccgaatcgaa
    19681 aatcgcttgg aaaacagtat tcagcagctt atcagccgct tgaacacgct ggaagaccgc
    19741 taccgcagcc cgatggccga gcttgcccga gaagtggaaa agtggcaaag caaagtcaat
    19801 gcccaccttg aaaatatggg ttttggaggc tgaaatggcg gcacagacag gctataaggc
    19861 gagcgggttt tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat
    19921 ttaggggatc gcggttcggg tgtccgcacc gcttaatacg tcgtcgtcca cgaactgacc
    19981 catttgctcg aacgccatcg caacgcccgt tttatggcgc atatggacaa ctttctccca
    20041 aactggcaaa gcatcaaaca acagcttaat gccttggagt tatttgcaca aatatataat
    20101 ttaacataat atatattatg cgaactatcg gaaacagttg tacgtgtccc tgtggtcttt
    20161 ccaagtagga aaattaaagt atggacaatg cggctgaatg tatagcccgg agcatccgcg
    20221 tatccgaagg tactgaacga tatgccctgc atccctttcg acgcttccat ccagccggta
    20281 ttgggaaata cgttgacctt caatacgata tacaggcagc cgacacacag gatatgccgc
    20341 tgctttttca tcatttcttc tgtcaaatcc ttggaacagt tgatttgaaa acacggcttt
    20401 acatacgtcc caattctctt tgcagtgctt ggatattttg ctgcctgtcc aatacattgc
    20461 tttgcaatgc gttgatttct tgatggttga tgttgccgcc ttttgccaga cgtgcttgtg
    20521 ataacatttt ttgggcttca accaatgctt tgcgttcgtt gctcaattct gtttcgagaa
    20581 tggagcgtct gctgttgttt gagggtgctt gttgcggcgg cggcgtattg gattttgccg
    20641 gcttggatac tgttttgacc ggggctttat atttgacagc ctgtccgccg tttgacggtg
    20701 atggtgtcgg ttcggatgtt tggggcggga tatagcgttc gctgctgtag ttgccgattg
    20761 ggggcaaatc ggttgagagg cagcttttgg acggcttggt ggtgtaaacg gtttctccgt
    20821 tgattgtgca ggtgtagatt ttggccgcat tcgcacccaa tgggcttgaa atcagggaaa
    20881 agttgattag gattaagggg agttttgatt tcataatgtg cttgatgctc ggataattat
    20941 ttgatttttt ggtatttttt gtaatgcttt cttggcgttt tacgggcttt tcttttcggt
    21001 tggttgcttt tgattttctt tcggggcttt ggcagcctgc ttgttctttt tcccggcagg
    21061 ttttttgttt ttctttttac tttctatgtg tattttggct tcttaactga gttttttaat
    21121 tttcaggcgg tatccgcctc cctgatggct gctgatttta ggtaaatccg catcggcgca
    21181 cgatcctgct ggggctgatg tatatacgct tttgccattt gagttgcaat ggtatacgga
    21241 ggcttgcgcc gccgcgccag aaagggacag gagacccaag gcggcaaaaa gtctgatgtt
    21301 tataccggta aaaggcggtg ataacgcccg aattataccg ttattggcag gcaaagataa
    21361 gcaccctgcc cgcgcttctt tatcgctcgg caaactgttt ctcgatttca gtttcaatcc
    21421 gttccaattc ttcttgacaa gccagccaaa cctcttcgat ttgggcaagt tgtgttttga
    21481 cttttgccag ctgggataag gtattttgca atttttcttt gtttgcttca aggtaagcgt
    21541 cttcttgtgc taaaaatgct tcacatgccg tctgaatttc ggaaagctgc gccatttctt
    21601 tttcggcacg gtctattttc tgctgtatcg gcttgccgcg tcgggctttt tcctgacgga
    21661 tttgcgcttc gatgcgcttg gtgtctttgc ggctttggct ttgtgcggat gctgctggtg
    21721 cggcggcgtt ttcctgtgcc aaacgccatt ggcggtaatc gttcaaatcg ccgtcgaagt
    21781 tcttcagacg gcctttgtcg atcaggagga agctgtcggt agtcgcttca agcaggctgc
    21841 ggtcgtgcga tacggcgatt aatgcgcctt ggaaactttg caacgcgagc gttaaggcgt
    21901 ggcgcatatc caagtccaaa tggttggtcg gttcgtcaag cagcaacagg ttcggctttt
    21961 gccagataat catggcaagg gcaaggcggg ctttttctcc gccggaaaaa ggttcggttt
    22021 tctgtaacgc catatcgcca acaaaattga agccgccgag gaaatttcgg atttcttgtt
    22081 cgcgtacttc gggagaaagc tgctgaatat gccaaacagg gttttggtcg gcgcggatgg
    22141 tatcgagttg gtgttgggca aaatagccga tattgagttt ttcggaacgg acgatgctgc
    22201 cggagagcaa atcgattttg cccgccaagg ctttgataaa tgtagattta ccgctgccgt
    22261 tgacacccaa taaaccatag cgcgcgccgc tttccagcga cagggtaatg tcgtgcaaaa
    22321 cagttttgcc ttcgtaaccc aaatctgcgt gttccagctt cagcaaagga ttgggcagat
    22381 gctcgggatt gtaaaactca aaggaaaact cgctgtccag atgcgcgggc gcaacgcgtt
    22441 ctagctttgc caaagccttc atgcggcttt gcgcttgaac ggctttggtg gctttggctt
    22501 tgaagcggtc gataaaggat tgcaaatgtt tgatttgcgc ctgctgtttg acataggcag
    22561 cttgttgttg cgcgagacgc tgcgcgcgtt cgttttggta aaaatcgtaa ttgccgccgt
    22621 attgcgtgag tttttgctgc gataattcaa tggtttgggt agttgccgcg ttgagaaaat
    22681 cgcggtcgtg ggaaatgatg atttgcgtgc agggtaaaga agcaagatgg ttttccagcc
    22741 acaagacggt ttccaaatcc aagtggttgg tcggttcgtc caaaagcagc aaatcggcgc
    22801 ggcaaatcag ggcttgggcg agattgaggc gcatacgcca gccgccggaa aaggatttga
    22861 cggggcggct gtgttcttct tgtgaaaaac ccagcccgtt caacaatttt gccgcgcggg
    22921 ccggcgcggt ataagcgtcg atttcttcca atttggcatg atattccgcc tgcttcatgc
    22981 cgtcattttg cgcttccgcc tgcctcaatg ccgtctgaaa agcctgcaac tcggcatcgc
    23041 cctgcaaaac gtaatccaaa gcggaaatat ctaaatcggg cgtttcttgg gaaacggaag
    23101 cgagccgcca gtttttcgga atcgagacat cgccgccgtc ctgagtgatt ttacccttga
    23161 ttaaggcaaa caggctcgat ttgcccgttc cgtttttgcc gatcaaaccg acgcgctgac
    23221 cgggattgac ggtagcgttg gctttgtcga gcaggacttt caaaccgcgt tgcagggtga
    23281 ggtttttgat ttcaatcata acggaaacat cgtcgggcgg aaaaagcccg tattttacct
    23341 gaaagtcagt gccgatgccg tctgaaatgg gaaatttacg gctgaagcca agcccaagcc
    23401 ctgcgccctt ccgagtgcag gaaaaccaat gtcctgaatg ccgaatcggt attcatgcat
    23461 tccacgctga ttccgattcg ggaaaaatcc gccatgattt tgggatggat aaactcctga
    23521 gccgcgcccg tcccgataat caatatttcc ggatagtcaa caggtttgat gtcggacaac
    23581 aggttttccg gagtcagatc ggacaaggtt cggcattgcg acaggcagac cgaatcctta
    23641 tgtacaagca cgggtttatg gaaactttgc cccgtcagcc ggattccgcc cgcaccgcat
    23701 tcatattccg caaactgtcc gtctatcgga ttttcttcaa acaacatttt tttaccccgt
    23761 cgccgcatca tctacaccga aaggggtgca aaatcaggcg aattcatgta ggattggcag
    23821 atttcatctg acccgcctgc cgatttcaga cggcatttga ttcaaagtgc ggcacaatta
    23881 tatcgacagc ggatattttc gtctttcaat atttacattt cagtcggctt acaaggagac
    23941 acaatgaagc cagtaaacat cggtctttta ggtttgggta cggtcggcgg cggtacggct
    24001 gccgtgttgc gggacaacgc ggaggaaatt tcccgtcgct tggggcgcga aatccgtatt
    24061 tctgccgtgt gcgatttgag tgaagaaaaa gcccgacaaa cctgcccgtc cgcagccttt
    24121 gtcaaagatc cgttcgaact ggtcgcacgt gaagacgtcg atgtcgtcgt cgaattgttc
    24181 ggcggtaccg gcattgccaa agatgcggtg ttgaaagcca ttgaaaacgg caaacacatc
    24241 gttaccgcca acaaaaaact gctcgccgaa tacggcaacg aaatcttccc gctggcggaa
    24301 aaacaaaacg tcatcgtcca atttgaagcg gcagtagcgg gcggtatccc aatcatcaaa
    24361 gccctgcgcg aaggtttggc ggcaaacagg attaaatcca tcgccggcat tattaacggc
    24421 accagcaact tcatcctctc cgaaatgcgc gaaaaaggca gcgcgtttgc cgatgtactg
    24481 aaagaagcgc aggcattggg ttatgccgaa gccgatccga ccttcgacat cgaaggcaac
    24541 gatgcgggcc ataaaatcac catcatgagc gcactggcat tcggcacgcc gatgaacttt
    24601 tccgcctgct acctcgaagg catcagcaaa ctcgacagcc gcgacatcaa atacgccgaa
    24661 gaacttggct atcgcatcaa actgttgggc attacccgca aaaccggcaa aggcatcgag
    24721 ctgcgcgtcc accctaccct gattcccgaa agccgcctct tggcaaacgt caacggcgtg
    24781 atgaacgccg tgcgcgtcaa cgccgatatg gttggcgaaa ccttatatta cggcgcgggc
    24841 gcgggcgcat tgccgaccgc ttccgccgtg gttgccgata tcatcgacat cgcccgcctg
    24901 gttgaagccg ataccgccca ccgcgtaccg catctggcgt tccaacccgc gcaagtccaa
    24961 gcgcaaacca tcctgcctat ggacgaaatt accagcagct actacctgcg cgtccaagcc
    25021 aaagacgaac cgggcacgct ggggcaaatc gccgcgctgt tggcacaaga aaacgtgtcc
    25081 atcgaagcac tgattcaaaa aggcgtgatt gatcagacca ctgccgaaat cgtgattctg
    25141 acccacagca cggtcgaaaa acacatcaag tcggcaatcg cagccatcga agcactggat
    25201 tgcgtggaaa aaccgattac catgatccgc atggaaagcc tgcatgactg agccgaaaca
    25261 cgaaatgccg acggaagagc aggttgccgc gcgcaaaaaa gcaaaagcca aaatccgcac
    25321 catccgcatt tgggcatggg tcattttggc gttgctcgct tcaaccgccc tgctctccca
    25381 atgcgcgatg tccaaaccgc aggcaaaaca gaaaattgtc gagtcttgcg tgaagaatat
    25441 tccgtttgcc gaaaaatggc aaaacgattt gcgggcccgc ggtttagatt caaacaatac
    25501 ccgccttacc gtcgactact gcaaatgtat gtgggagcag cctttggaca gattgagcga
    25561 gaaacagatt agttccttcg gcaaactcgg cgcacaagaa cagcttgacc tgctcggcgg
    25621 cgcaaatgcc tttgaaacgc gagacaagca gtgtgttgcc gatttgaaat cagaataatg
    25681 tggaccgata aaaaagccga ttctttaaag aatcggcttt tttcataaaa aacggcttac
    25741 agtgcgtctt tcaatgcttt acctgcacgg aatttaggcg ttttggcggc ggcaatggtc
    25801 agaggctcgc cggttttggg gttgcggcct tggcgttccg cacgttcgcc cacgtagaaa
    25861 gtaccgaaac cgaccaaagt aacggtgtcg ccttgtttca gggcggtggt tactgcattg
    25921 gtagtggcat ccaaagcttt ttgtgcggcg gctttggaaa tgtcggcttc ttgagcaatc
    25981 gcttcgatca attcagactt gttcacaatc agtcccttcc tgtcttaaaa aatgatgaaa
    26041 tgcccgaata ctcggggttt gtactgcttg agcaactttc gctttatagc aattctgaaa
    26101 ttgccgtgtc aagcaaaaaa tacggaatca ccctatttga caggctttca ggacgaaacc
    26161 gcatttttac aacacatttc ctgcgtttca atgtttggtt gccctgctgc ggggttttgg
    26221 ttttgaagcg gattccgcca ccgcttccgc accagaaggt tctgcccaag gctcaggctg
    26281 gctttccaaa cccagagcca atacctcgtc tatccatttg accggatgga tggtcaggcc
    26341 ggttttcacg ttttcaggga tttcttccaa gtctttgaag ttgtcttttg gaattaaaac
    26401 gtgtttgatg ccgccgcgaa gcgcagccaa cagtttttcc ttcagaccgc caatcggcag
    26461 gacttcgccg cgcagggtaa tttcgcccgt catcgctaca tcggcgcgta ccgggatttt
    26521 ggaaaatgca gacaccatcg ccaaagtcat ggcaatgccc gcactcggac cgtccttcgg
    26581 cgttgcacct tcgggaacat ggacatggat gtcttttttc tcgtaaaaat cagaagccaa
    26641 gcccactgat tccgcgcggg agcggacaac cgaccacgct gcagacacgg attccttcat
    26701 cacatcgccc aactggccgg tgcactgaat cacgccctta cccggcaatg ctgcggcttc
    26761 gacggtcagc aattcgccgc cgacttctgt ccacgccaga ccggttactt gtccgatacg
    26821 gttttcgctt tcggcaacgc cgtaatcgaa gcggcgcaca cccaaatagt cgtgcagatt
    26881 tttctcattt actttaaccg ctttaggttt ggctttgctg gttttcttgg tttcagacaa
    26941 cctcttctta tcttcgtcca aggtaatctg catcaccacc ttgcggcaga ttttggcaat
    27001 ctcgcggtcg agcgaacgca cgcccgcctc gcgggtgtag taacggataa tatcgcgcac
    27061 cgcgctttct tcgacgagca attcgccttc tttcacgccg ttgcgcttca tttgcttcgg
    27121 cacgaggtac tgcatcgcga tattgatttt ttcgtcttcg gtgtagccgg acagacggat
    27181 gatttccata cggtcgagca gcggggtcgg gatattcagg ctgtttgaag tcgcaataaa
    27241 catcacatcg ctcaaatcgt aatccacttc ggcataatga tcggcgaact tgttgttttg
    27301 ctcgggatca agcacttcga gcaacgcgct ggcgggatcg ccccggaagt cgctacccaa
    27361 tttgtcgatt tcgtcgagca ggaacaatgg gttttttacg ccggcttttg ccatattctg
    27421 caaaatctta ccgggcatgg agccgatata ggtgcggcgg tgtccccgga tttcgctttc
    27481 gtcgcgcacg ccgcccaaag ccatgcggac atatttccgc cccgttgctt tggcgatgga
    27541 ttcgcccaaa gaggttttgc ccacgcctgg agggccgacc aggcacagaa tcgggccttt
    27601 gagtttgtcc atacgttttt ggacggcgag gtattccaaa atccgttctt tgactttttc
    27661 caggccgtag tggtcggtat ccagcaccaa tccggctttg gcgatgtctt tgctgacgcg
    27721 ggattttttc ttccacggca gttcgagcag ggtgtcgatg tagttgcgta cgacggtgga
    27781 ttcggcagac atcggcggca tcattttgag ctttttcagt tcggacaggc atttttcttc
    27841 cgcttctttg gtcatacccg cctttttgat atctgcttcc aaggcatcca gttcgccgtt
    27901 ttcgtcttct tcgcccagtt ctttgtgtat cgctttaatc tgttcgttca gataatattc
    27961 gcgctgggat ttttccattt ggcgtttgac gcgtccgcgt atgcgttttt cggcctgcat
    28021 aatgtcgagt tcggattcca gctgtgccag caggaattcc atccgtttgc cgatttcggg
    28081 aatttccaaa atctgttggc gttgcgccag tttcaactgc aaatgcgctg cgaccgtatc
    28141 ggttagccgg ctgttttcgg caatgccgtt aatgctgccg ataatttcgg cggggatttt
    28201 tttattgagt ttggcgtatt gttcaaactg cgccaacagg gtgcggcgca cggcttcgag
    28261 gtcggtattg ccgcccgtgt cttcttccac gaccgcctct atatgggaaa cgaacagccc
    28321 gcccgtgtct tcaatggtca gaacacgtcc gcgatacagc ccttcgacca atacttttac
    28381 cgtgccgtcg ggtagtttca acacttgcag gacttgtgcg accgtaccgg tctgatacag
    28441 gtcggcggca accggttctt ctaccgccgc atcggtttgc gccaacagga aaaccggctc
    28501 ctcgcgggta atggcgtttt ccagtgcggc gatggatttc ggcctgccga caaacagcgg
    28561 cagaaccata tgcgggtaaa cgacgacatc ccgcaaagga agggttgcca aggcggcata
    28621 ttcctcaaaa tgcttttctt tttgtgtcat aggtactctc ttgtgtctga cagattgccg
    28681 attttcgcgt acattggggt tgaaggtatt atttcaagca tatgtggttt atttatggag
    28741 tttgatgcga tgccgtctga aacattccgg cttcagacgg catgggcttg gaaagacaag
    28801 gcgggaacaa aaaactgttc tgtgttgccg ctccttgctg taccatccgt atggtttgcg
    28861 gttctgccgc cctattttca aaacatgacg caggtattcc atgtcttctt ttatttttcc
    28921 cgataacggg ttcgaatggc gcaacgaaag tctgcaaaag ccgcccaaag gttggcatta
    28981 tgtccgcgaa agtggtgcaa acggcatttt gaaggctgcc tatcaaaata ttgcaactgt
    29041 ctggcagggc gatttccaca atgccaaaca ggtgctttcc gcaatgaaga agaaggtccg
    29101 caaacctgcc gccgtccgtt ccgatgcgga tatcgccgcc cttttccacg cccaccgtat
    29161 gaagcaggcg cagcagagcc gtattctgaa tatgcttgcc gttgaaatcc gccccggttt
    29221 tgtgttggac aacaaacgcg cgcccgatat acgcgccgcc ctgctcgacg tgtacggaga
    29281 ggcggacggc aaaccgtttt tcctgccgct caatctgctg ctggggttta tgggtgcgca
    29341 cgagtggcat aagaaagggg ttgccgttcc gcagctgggc ggcagcatac acgttccttt
    29401 cggcgtattc tcgccgttgc gcggcgaata cctcgacctg ctcgcccatg cgccgtcaac
    29461 gggttttcag acggcattcg atatcgggac gggctcgggc gtgcttgccg ccattttggc
    29521 gaaacagggc attccttccg tcatcggcac ggataccaat ccgaaagccg tcgcctgcgc
    29581 ccgtgccaat attgctcgtt tgggctttga aaaacaggtt gagatacggg aaaccgatct
    29641 gtttcccgaa gggtttgccg atctgattgt ctgcaatccg ccctggcttc ccgccaagcc
    29701 gacttccgcc gtcgaatccg cgttatacga ccccgaatct gcgatgctgg ctgcgttttt
    29761 gcgggatgcg ccgaaacatc tgaatcccga cggagaaatc cgcctgatca tttccgatct
    29821 tgccgaacat ctgcacctgc gtccgtccga ttttctggat aaggcatttg ctcaggcggg
    29881 tttgcgcgtt gcggatgttt tgaaaaccaa accggttcac aaaaaagctg ccgatccgaa
    29941 caatccgctg gcttttgccc gaaacaggga aactactttc ctgtaccgtt tgaaaaaggc
    30001 ataaggggcg gcggtgcgct tccggcggca ggaatctgaa cgcccgcgcc gccggcacgg
    30061 catcattcgg gcggattatc cttgtgaaaa tacccgctca agcatactgc ccaatgccgt
    30121 ctgacgcgtt ttgacagtgg cggcagcttg ccggaaggtt tcttctccgc cgaagaatac
    30181 gaacttctcc ctcgcgcggg taatggcggt atataacagc tccttactca atccggacaa
    30241 tgcatcgtcc ccttcgtccg aaggtgcggc ggaaggcggc agcagccaga cttcccgata
    30301 ttccgaacct tggcttttgt ggacggtcat ggcgaatgcg ggttcaaatt cgggcaggca
    30361 gcttaccgct acctttttaa atccgtccgc atcggcaaaa taggcggcaa ggctgccctg
    30421 ccgtccgaca tcttccataa tcagtccgat gtcgccgttg aacagttcaa gcgcgtagtc
    30481 gttctgcctg atcataatcg gctctccggc aaaatatgcc aaatgttccg gtatgttcat
    30541 tttgcggcgt acatggcggc aataggcttc gttgaagtct tccgcatcct gccgccaagc
    30601 tgccagaacc acgatatccg aaatgcccgc gtatgcggct tcgatattgc cgtcttttac
    30661 cgcctgccaa taggctttgt gtgcccggta caacctttcg actcgagcgt tcggactgca
    30721 ttccgaatgt tccagttcgt ccggaaaccg gtcaaacaat gcccacgccc cttcatcgcc
    30781 cgatacggcg gcacgggcaa ggcagccgat gccgctgttg tcgccgaagc ggtggctgaa
    30841 cgacagatgg gcggtgtttt gcgccaacac gggtggattt gcgctgacgc tgaaaccgtg
    30901 ttccggaagg aagccggcca gcctttggtg cgtttctccg tccaaaacgg ttttttgtga
    30961 caaaacggac agcaccgccc ctattccgac ggacgggagc tggttttcat cccccagcag
    31021 aatcacgcgc gcgccggttt tgaccgcttt taaaagttgc agcatcaatg ccgtatccag
    31081 catagaggct tcatcgataa ccaatacgtc aaacggcagc gggtaaacag ggttgaacgc
    31141 cgcctgcatt ttgggcgggc gcagcttcag cagtcggtgg acggtttgcc cttccagttt
    31201 gagcaaatgg cggcggacgg cctccagcgc gtcaaaaccg ttgattgcac ggtgcagcgc
    31261 gcgcgccata tgtgccgccg ctttgcccgt cggtgcggca agcgcgatgt tgggaagatt
    31321 ttcgtcttta ccgcaaatca gcgccagcag tttggcaacc gttgtcgttt tgcccgttcc
    31381 cggcccgccg gtaatcacca taaaagactg caacagtgcc aaggcggcgg catcgcgctg
    31441 cccttcgctg cccgtgcctt gaaaccattt tgcgaggttt tgcctcgcgc ctgccgcgtc
    31501 gggggcggac gtgtcggctg ccgccaagcg ttttatctcg gcagccaaat cgtattccaa
    31561 ctgccacatc ctgcccaaaa acagccttct gccttccaaa atcaaaggcg cggcggatgt
    31621 tccgacaacg ggtgcgagtg ccgacagcgc gtcagcctcg ccaccgctca aacggataaa
    31681 cgaatgaccg ttttgcaatg cctgaaacag gcgttcggtg cagtttgcaa gcacttcgtc
    31741 gccggaaccc gcatagtgtt ccaaaaaacg gattgccgcc cttgccgccg cttgggcaaa
    31801 ttcatctgtc tgccgttcca ttttattcct tatccaaatg ccgtctgaag gcgtggggct
    31861 tcagacggcg cggtgttgtt tcggctgttt aggcgtttgc gccctgtttg gggtgcaggc
    31921 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
    31981 cgaccaccag ccccgttccc aattctgttt tcagttccag catcatatcc aaaacattcc
    32041 tggcgttcgc acgatcgaga ttgccggtcg gttcgtcggc aagcaggcat ttgggttggg
    32101 taaccaaggc gcgtgcaatg gcggcacgct gccgctcacc gcctgaaagt tcgcccgcgc
    32161 ggtgcgtcga acggtgtttc agtccgacct tttccagcat cgccattgcc gcctccgcag
    32221 cctcttcacg gctttttttg ccgatcagaa gcggcatcat cacattttcc agtgccgaaa
    32281 attcaggcag aagatggtgg aactggtaca cgaaaccgag atggcggtta cgtaaatcgc
    32341 ccaaacgccg ctggtttaag gtacgcaaat cctcgcccat cagcagcacc ctgccttcag
    32401 acggcatatc cagcccgcct agaatatgca gcagcgtcga ttttccgctg cccgaagaac
    32461 cgatgatgcc ggtgctttcc cccgcgcgga tttccaaatc caagccgtgc agcaccagaa
    32521 cgtccaaacc gccgtcccga tagcgtttgc ccacgccttc gcatttcaaa atcaactcac
    32581 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccggctcgg gtagagcgtc
    32641 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
    32701 tcgctgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
    32761 ttttcaaaaa acgccacgac cctgccgacg ttccaaccca aaagcacgcc gcagaccaca
    32821 cccgccaacg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
    32881 ccgccagggg aaagacccaa agtccgtaaa atcgcaatgt ccgcctgctt ttccgtaacc
    32941 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggatg
    33001 atgaacatca tccgtttttc caactcgacc gcctcaaaat agctgcggtt gctgaacgtc
    33061 caatcgcgta cccaaaccgt atccctttgc gcctccggaa tcagctttgc cgtcaaggcg
    33121 ggggcgtttt gcgggtcggc gagtttcagc cgcagccccg caacttcctt atccaagcgg
    33181 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtcaac ctcgtaaaca
    33241 cccgttttaa ccagaccgac cacggtaaac tgtttcaacc tcggtacgac tccggcgggc
    33301 gtaacattgc cctccggcgt gatgacggta actttattgc cgacttccgc ccccaaagcc
    33361 tccgccaagc cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaatttg
    33421 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
    33481 cgtatctgca cgcccctgat ttcgcccgca ttggccagca atgcctgatt ggaaacatag
    33541 ggcgcggcag ccaaaatacc tttgcggttt tcggtaaacc gaagcaggtt gcgccaatcc
    33601 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
    33661 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
    33721 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
    33781 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
    33841 tgtctgcaaa cgtgccgccc ccggacggcg gtttgggggc gacggcaaaa gtttattgta
    33901 ccgtaaaacc caggcagcgt ccgaacgccc gagtgcgggg gacggggcgg cagagcgggc
    33961 ggaaaacttg cacaacgcgt caaactgccc tatcctttcc ttcagaaaaa ccgtttcttg
    34021 aggaaaacaa tgaatatccg aactgctttt gctttgtgcg ccatcgcctt atccgccgct
    34081 gcggctgcct acgccaaaga aatcaaaatc gatgccaaca acacgcctta ttccgaagcc
    34141 gacgcgcaaa agctggcggc aacggcagtc ggtatgggtg ttaaggaacc tatcagcctg
    34201 aacggcggca gcggcagcat taccgtgtcc ggcagcagcg cgacgcagtg cgtgttcaaa
    34261 gtcggcaacg gaggcgcgtt gcagattcaa gggctaaact gcaagtaaac cgcccggaaa
    34321 aatgccgtct gaaggcttca gacggcattt tgcattggcg gcgttatgcc ccgccttctt
    34381 taatcaggcg gcgttcgtac accgcctgcg ccagcgttcc cgcatcgaca tattccaatt
    34441 cgccgcccaa gggaatgccc tgcgacagcc tgctgacttt gtaaggcagg tttttaaaaa
    34501 actcggacag gacatacgcc gtcgcattgc cttctgcggt aaaagcggtt gcaataatga
    34561 tttcttcgac ttccccgccg cccagccgtt gcgccagcct gtccaatgcg atggcggata
    34621 cgtccattcc caatgccgta ttgatttgcc ccatcaggac gaaatacagc ccgtcgtggc
    34681 agtttgccgc ttccatattc gacacgtcgg caggcatatg caccaccatc agccgtcgcc
    34741 cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc ttcgcaaaac gtgttgcacc
    34801 tcgcgcaatg atcgacctgc ttcaatgccg tctgcaaggc atccaccagt ttttcagcct
    34861 ctttgcgctt gtgttggagc aaatgatacg ctatccgctg tgccgatttc ggcccgacgt
    34921 tgggtaaaac cttcagcgcg tcgatcaatc cttggaaggc atcttgtttt ttgtggctca
    34981 tcatattccg ccgtatggga aaacggccgg aatattccga ccgttatttt gtcaacaaaa
    35041 gtgtcaatta ctgaccgtcg ccgttgtcga ccgattgcgc tcctttggtc tgtttgattt
    35101 tgccgttgaa ataacgtatc aacaagtcga aagtattggc agactgctgt tgcgccaaag
    35161 cctgttttgc aagcggaagc tgtgcggcga tatcatccgg cggggttaca gcctgtactt
    35221 cgacaatcac gggtgccggc agaccgatca gcctgacgta ggcgggtttg ccgtttgccg
    35281 gttttgcttt cagcagttcc gcataagcct cgggcggcat ggactgcctt gcctgctgtg
    35341 cgcccaaaac ggacacttcc gaccatttca cgtcaacagc cttgccgccg ttcagttggg
    35401 taagcacgtc ttttgccttg ttttcggcaa gtttggcggc ttcggtacgg atataagcct
    35461 gacgtaccgc gtctttggct tcggcaaacg gcagggtttt ctcttcgcgg acttctttgg
    35521 cgcggacgac ccacgcggtt tcgctgttga tggtcagcac ttcggaattg tgttttttct
    35581 tcaatacgtc gtcgctgaat acggcattga tcaggttttc gggcataccg gacatttgcg
    35641 catcctgcct gctcagccaa gtttcttggg tttcgacttt caaaccgctg tttttggcgg
    35701 cttcggcaag cgaggaagga tggttgaacg catcatcgcc cagcttttct tttgccttat
    35761 tgaagtcggc aaccgccttt ttcattttca attcgttttc gacggcggct ttttcctgct
    35821 cgaaagaagg tttggcttca tttgccggca aacgcgccac gcgctcttca aacgcatttt
    35881 tcacttctgt ttcgctgacg gtctgtttgt ctgcaaagtc tttcagattc aaggcgacat
    35941 attccaattt gaccgctttg ggaagcaggt agtctttttt gtttgcgtta taaaacttct
    36001 gcaaatcggc ttcagacact ttgacttggg cgatgaattc gtcggggttg aaagtgtgcg
    36061 aacggatggt gcggttgacc tgcgtcagcc tgatcagctg ttccgcctgc gcgtcgccga
    36121 ccaatacgcc gttttggacg aggtttacca aattctgcaa ggcaaactga tcgcggattt
    36181 cttcgacaaa ctggtcttca gacatatgac gttgggaaag gtagcggttt aaaagcgcgt
    36241 ggtcgaattt gccgtttgcg tcgtggaaat tgggatcgtc cacgataatc tgcttgattt
    36301 gttcggaaga aaccgaaatg cccatcagct tcgcgccctg tttcaggtag gcgcgttgta
    36361 gcagggattg gaacaccgcg tcgcgcgaag ggccgccgcc gtccgcctgt tcgttctgta
    36421 tggcgttgtt gatggagtgg tcgctgattt tttcgtcgcc cacttggacg atgtagtcgg
    36481 cacccggatg ggataccgtg ctgaccccga agccgacgaa ggttaatgca atcaggccca
    36541 aaaggacttg ggcgggcgtt ctgtattttt cgatggaatg gaacatattt taaatcggga
    36601 tatagaatgg gaaattcaag tcgggtattg taacggtttt tatccctgtc tgcacggggc
    36661 ttgccggttg aagatgccgt cgtaggtttc ttcgctgaag ctggcgtaaa ccttgccgcc
    36721 gcactccaat acgggacgct tgatcaggct cggcatttcg gacatcagtt tgacggcctc
    36781 cgccgtcgag gacagcactt tttgctgtgt ttcggcatcg agtttgcgcc agcttgtccc
    36841 gcgtttgttg agcagggttg ccaaaggcac ttgttccagc cacgagcaga tttccgcttc
    36901 agacggcctc tgttttttaa aatcccgaaa ttcaaactcc aagccgtatc cggcaagccg
    36961 gtttttggct tttttgaccg tatcgcaatt tgggatgccg tgaaggatta tcatttggaa
    37021 accttttgtc tgaaataata aaacggatat tttactataa gtgtctgaaa atttgcccgt
    37081 ctgtttcaga cggcggggcg gttatgttac aatccgaaaa ttcgaaaaat ttaatctctt
    37141 gttcaataaa ggctttacca atcatgattt ctaccaacgg catcaccatg cagttcggcg
    37201 cgaagccgct gtttgaaaac gtatccgtta aattcggcga aggcaaccgc tacggtttga
    37261 tcggcgcgaa cggctcaggc aaatccacct tcatgaaaat cctcggcggc gatttggaac
    37321 agacagccgg cgaagtggcg attgaaaacg gcgtgcgttt gggtaaattg cgccaagacc
    37381 agtttgccta cgaagatatg cgcgtgctgg acgtggtaat gatgggtcac accgaaatgt
    37441 gggcggcgat gaccgaacgt gatgcgattt acgccaatcc cgaagccacc gaagacgact
    37501 acatgaaagc cgcagaactg gaagccaagt tcgccgaata cgacggctac accgccgaag
    37561 cgcgtgccgc cgagttgttg agcggcgtgg gcatttccga agatttgcac aatgcaacca
    37621 tggcagaagt tgccccgggc ttcaaactgc gcgtattgct ggcgcaagcc ctgttctcca
    37681 agccggatgt attgctcttg gacgaaccga ccaataactt ggacattaat accatccgtt
    37741 ggttggaagg cgtgttgaac cagtacgact ccacgatgat tatcatctcg cacgaccgcc
    37801 acttcttgaa cgaagtctgc acgcatatgg cagatttgga ttacaacacc atcaccatct
    37861 atccgggcaa ctacgacgac tatatgctcg cctccgccca atcgcgcgaa cgcgccctga
    37921 aagacaatgc caaggcgaag gaaaaattgc aggaattgca agaatttgtg gcgcggttca
    37981 gtgccaacaa atccaaagcc cgtcaggcaa ccagccgtct gaaacaggca gacaaaatca
    38041 aatcggagat ggtcgaggtc aaaccctcta cccgtcaaaa cccgtatatc cgttttgaag
    38101 ccgatgaaaa agccaagctg caccgtcagg ctgtggaagt tgaaaaactg gcgaaacgct
    38161 ttgaaaccca gttgtttaaa aacctgaact ttatccttga agcgggacaa cgcctcgcca
    38221 tcatcggccc gaacggcgcg ggcaaatcca ccctgctgaa actcttggcc ggcgcgtaca
    38281 accccgaata ttcagacggt ctgctgccgg acgaaggcag catcaaatgg gcggaaaaag
    38341 ccagcgtcgg ctactatccg caagatcacg aaaacgactt cgatgtcgat atggatttga
    38401 gcgaatggat gcgccaatgg gggcaggacg gcgacgacga acaagtcatc cgcggcactt
    38461 tggggcgttt gctcttcggc agtaacgatg tcgtgaaaaa agtgaaggtt ctctccggtg
    38521 gtgaaaaagg ccgtatgctt tacggcaaac tgttgctgtt gaaacccaat gtcttagtca
    38581 tggatgaacc gaccaaccac atggacatgg aaagcatcga atccttgaac atggcgctgg
    38641 aaaaatacaa cggcacgctg attttcgtct cccacgaccg tcagttcgtt tcttccctgg
    38701 ctacccaaat cattgaattg gacggcaaag gcggatatga acactacttg ggcgattacg
    38761 aaagctatct cgagaaaaaa ggcgtagcat aaccgccggt tggaacaatg ccgtctgaag
    38821 cggcttcaga cggcattgtt gataacttta aaataggaag catatgcaga cttatctcgt
    38881 cggcggtgcc gtccgcgatt atcttttggg cttgcccgtc aaagaccgcg attgggtggt
    38941 cgtcggcgca gacgcacaaa ccatgctggc gcaaggcttc cagccagtcg gcaaagactt
    39001 tcccgtgttt ctccatcccg aaacacacga agaatacgcc ctcgcccgca ccgagcgcaa
    39061 aaccgccaaa ggttacgtcg gtttcagttt ccacgccgac aaagacgtta cgctggagca
    39121 ggatttgatg cgccgcgacc tgaccatcaa cgcgatggcg caagatgcgg acggcaagat
    39181 tatcgaccct ttcggcggac aacgggattt ggcggcaggc attttgcgcc acgtttcccc
    39241 agcctttgcc gaagaccccg tccgcatcct gcgtactgcc cgctttgccg cgcgttacaa
    39301 gtttgaaatc gccgaagaaa ccataaagct gatgcggcag atggtggaaa acggcgaagc
    39361 ggacgcattg gttgccgaac gcgtctggca ggagtttgcg aaaggtttga tggaaaaaaa
    39421 tccgcgcaaa atgattgaag tgttgcgcga atgcggcgcg ctcaaagtct tgctgcccga
    39481 agtcaatgcc ctcttcggcg tgccgcaacg cgccgactac catcccgaaa tcgacagcgg
    39541 catccatacc ctgatgacgc tgcaacgcgc cgccgatatg ggtttgagcc tgcccgaacg
    39601 ctatgccgcc ctgctgcacg acttgggcaa agccaaaaca ccgtccgaca tcctgccgcg
    39661 ccaccacgga cacgacctcg ccggtgtcga acccgtgcgc aaagtcaatc aaaggctgcg
    39721 tgcgccgaaa cattgtgccg agcttgccga attggtttgc cgttggcaca ttattttcca
    39781 ccaagtcgga cagcttaaaa gccaaaccat tctgaacgta ttgaaaaaaa ccgacgcttt
    39841 cagacgaccc gaacgctttc agacggcatt gaacgtctgc attgccgaca cgcaaggccg
    39901 tctgaaccgc gaacacacgc cctacccgca acgcgcgcac tggctcgcct tactcgaagc
    39961 cgccaatcag gcggattcgg gcaaaatcgc cgccgaatgc cgctcgcagg gaaaagcgca
    40021 ccttatcgcc gaacaaatcg accgggcgcg gctggcacaa atcgccccat tgcaaaaagc
    40081 gtttcgagcg gcgcaagaca aaacagaaaa acattaaaac gtccaatgca gccactttta
    40141 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
    40201 caaggcgagg caacgctgta ctggtttttg ttaatccact ataaagtttt gaggacgata
    40261 cccaatccaa gctttgcaac agccgccgcc atatccgcta taattcacgc ttcagccatt
    40321 ccgcccccga cataaaatca tgaccctgaa aaccgattta ttgcctaaaa tcaacaacga
    40381 agattatcaa cgcctcatcc tcaaacacag tgcggaattt agcggtggcg aaatccgcct
    40441 attgaacgaa atcctcgaaa aattcaattt cgacgttgtt caggcgcagg cgttggcgca
    40501 ggcggtgatg cagcaagtcc gcttcgaccc caacgcctat cacatcgaca gcgacgacga
    40561 agacaccaca ggcatctgcc cacactgcat caacccgcct atgccgcccc tgcgcgacta
    40621 tctcgtttgg cgcgaaacgc gcgggtaaaa cgcttttgac cgttatcttt tcaatgccgt
    40681 ctgaaacgcc gccgaccgtt cagacggcat acccgacaaa gggaacacta tgctgcaaac
    40741 cgacaacctg actgccgctc aaccgcaacg cattgttgcc gcccaaaccg cctccgcaca
    40801 ggaagaactg ctcgaacgcg ccctccgccc caaaacgctg gacgactata tcgggcagca
    40861 taaagccaaa gaacagctcg ccattttcat ccaagccgcc aaaaagcgcg gcgaagcact
    40921 cgaccacgtc ttgcttttcg gtccgcccgg gctgggtaaa accacactgg cgcacatcat
    40981 cgccaaagaa ttgggcgtaa atttgcgcca aaccagcggc cccgtcctcg aacgcgccgg
    41041 cgacctcgcc gcccttttga ccaaccttga tccgcacgac gtattgttta tcgacgaaat
    41101 ccaccgcctc agccctgttg tcgaagaaat cctctacccc gcgcttgaag actaccggct
    41161 cgacattatg ataggcgaag ggcccgccgc ccgttccgtt aaaatcgacc tgccgccctt
    41221 cacgcttgtc ggcgcgacca cccgcgccgg tatgctgacc aatccgttgc gcgaccgctt
    41281 cggcatcgtc tcccgcctcg agttttacga aaaccgcgac cttgccacca tcgtcagccg
    41341 ttcggcacaa ctcctgcaac tcgatatgtc cgaagaaggc gcggaagaaa tcgccaaacg
    41401 cagccgcggt acgccgcgca tcgccaaccg cctgttgcgc cgcgtgcgcg atttcgccga
    41461 cgtgaaaaac aacggcacaa tcgacggcgg catcgccgat gccgctttaa gtatgctgga
    41521 cgtggacgtg caggggctgg acgtgatgga caggaaattc ctcgaagccg ttttgcacaa
    41581 attcggcggc ggcccggtcg gtttggacaa tgttgccgct gccatcggcg aatcaacaga
    41641 caccatcgaa gacgttatcg aaccctatct catccaacaa ggcttcctgc aacgcacccc
    41701 gcgcggcagg atggcgaccg aacgcgccta cctgcatttc gggctgcccg tcgaaaaata
    41761 acgcaatgcc gtctgaaaca gagctaattt tcagacggca tttctatttc aatcattggc
    41821 gcaaggttca gcctgccgct tttttccagt tccgccctca tcgcatcaat caccgcctta
    41881 tagtctggtt tgccgaaaat cgcagaaccg gcaacaaagg tatccgcgcc ggcttgggcg
    41941 gcggcggcga tattgtcggt tttgatgccg ccgtccactt caatggcaat acgtctgccg
    42001 cttttctctt catagcggtc aagcatggca cgaacctgtc tgattttttc caaagtatac
    42061 ggaatgaagc tctgaccgcc gaacccgggg tttaccgaca tcagcaaaac catatccagc
    42121 ctgtccaata cgttttccaa cagatatacg ggcgttgccg gattcaacac cagccccgcc
    42181 tgacagccca tgtcccgaat caggctcaag ctgcggtcaa tatggcggct cgcttcggga
    42241 tggaacgtga tgattgatgc tcctgctttg gcaaacgact gaatcaggtc atccacgggt
    42301 tcgaccatca gatgcacatc gatcggcacg cttgcataag gcttcaacgc cgcgcaaacc
    42361 ataggcccga aggtcaggtt cggcacataa tggttgtcca tcacgtcaaa atggatcaga
    42421 tctgcacctg ccgcaatgac gctttccacc tcttcgccaa gccgtgcaaa atctgccgat
    42481 aaaatgctgg gtgcgatacg gtaagtagtc atgttttttt ccttcaatat ccttttat
//
LOCUS       DAWFKN010000008        40902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFKN010000008 DAWFKN010000000
VERSION     DAWFKN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.008"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(171..863)
                     /locus_tag="WGR46_000405"
     CDS             complement(171..863)
                     /locus_tag="WGR46_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0855414.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGI
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WGR46_000406"
     CDS             complement(971..2164)
                     /locus_tag="WGR46_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0855415.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGR46_000407"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGR46_000407"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0855416.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WGR46_000408"
     CDS             complement(3017..3946)
                     /locus_tag="WGR46_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0855417.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGR46_000409"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGR46_000409"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0855418.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WGR46_000410"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WGR46_000410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0855419.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7216..7674
                     /locus_tag="WGR46_000411"
     CDS             7216..7674
                     /locus_tag="WGR46_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0855420.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(7945..8160)
                     /locus_tag="WGR46_000412"
     CDS             complement(7945..8160)
                     /locus_tag="WGR46_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855421.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(8168..8371)
                     /locus_tag="WGR46_000413"
     CDS             complement(8168..8371)
                     /locus_tag="WGR46_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855422.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WGR46_000414"
     CDS             complement(8284..8643)
                     /locus_tag="WGR46_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0855423.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8696..9043
                     /locus_tag="WGR46_000415"
     CDS             8696..9043
                     /locus_tag="WGR46_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0855424.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            9271..9891
                     /locus_tag="WGR46_000416"
                     /pseudo
     CDS             9271..9891
                     /locus_tag="WGR46_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
     gene            9914..11365
                     /locus_tag="WGR46_000417"
     CDS             9914..11365
                     /locus_tag="WGR46_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ0855425.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11827..12183
                     /locus_tag="WGR46_000418"
     CDS             11827..12183
                     /locus_tag="WGR46_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0855426.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12174..12656
                     /locus_tag="WGR46_000419"
     CDS             12174..12656
                     /locus_tag="WGR46_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0855427.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12669..13262
                     /locus_tag="WGR46_000420"
     CDS             12669..13262
                     /locus_tag="WGR46_000420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0855428.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13252..14508
                     /gene="nuoD"
                     /locus_tag="WGR46_000421"
     CDS             13252..14508
                     /gene="nuoD"
                     /locus_tag="WGR46_000421"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0855429.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDVFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14508..14981
                     /gene="nuoE"
                     /locus_tag="WGR46_000422"
     CDS             14508..14981
                     /gene="nuoE"
                     /locus_tag="WGR46_000422"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0855430.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            15371..16672
                     /gene="nuoF"
                     /locus_tag="WGR46_000423"
     CDS             15371..16672
                     /gene="nuoF"
                     /locus_tag="WGR46_000423"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0855431.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            16672..17109
                     /locus_tag="WGR46_000424"
     CDS             16672..17109
                     /locus_tag="WGR46_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855432.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            17399..19660
                     /locus_tag="WGR46_000425"
     CDS             17399..19660
                     /locus_tag="WGR46_000425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0855433.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAMGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWADRLKKLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQATVH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLSLHTENAA
                     LGALMDTIELAGA"
     gene            19663..20739
                     /gene="nuoH"
                     /locus_tag="WGR46_000426"
     CDS             19663..20739
                     /gene="nuoH"
                     /locus_tag="WGR46_000426"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0855434.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            20819..21298
                     /gene="nuoI"
                     /locus_tag="WGR46_000427"
     CDS             20819..21298
                     /gene="nuoI"
                     /locus_tag="WGR46_000427"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0855435.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            21329..22036
                     /locus_tag="WGR46_000428"
     CDS             21329..22036
                     /locus_tag="WGR46_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855436.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            22072..22743
                     /locus_tag="WGR46_000429"
     CDS             22072..22743
                     /locus_tag="WGR46_000429"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0855437.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            22740..23045
                     /gene="nuoK"
                     /locus_tag="WGR46_000430"
     CDS             22740..23045
                     /gene="nuoK"
                     /locus_tag="WGR46_000430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0855438.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            23079..23654
                     /locus_tag="WGR46_000431"
     CDS             23079..23654
                     /locus_tag="WGR46_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0855439.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            23778..24119
                     /locus_tag="WGR46_000432"
     CDS             23778..24119
                     /locus_tag="WGR46_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855440.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            24168..26192
                     /gene="nuoL"
                     /locus_tag="WGR46_000433"
     CDS             24168..26192
                     /gene="nuoL"
                     /locus_tag="WGR46_000433"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0855441.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLTSPVLYLAAAGVAAAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26260..26580
                     /locus_tag="WGR46_000434"
     CDS             26260..26580
                     /locus_tag="WGR46_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0855442.1"
                     /translation="MSNTQTIRSADFTASRAWGALDIANMNGTTVRLHWTDQSYKWHV
                     NDGEEVFAVMNGEVDMHYRENGEEHIVRLKSGDIFYAGIGTEHVAHPCGEARILVIEK
                     EGSA"
     gene            26639..27802
                     /locus_tag="WGR46_000435"
     CDS             26639..27802
                     /locus_tag="WGR46_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0855443.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIQEVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKAKSWANEIVMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            27916..29412
                     /locus_tag="WGR46_000436"
     CDS             27916..29412
                     /locus_tag="WGR46_000436"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0855444.1"
                     /translation="MFSNYLLSLAIWIPIVAGVLVLATGPDSRAPFARVLALIGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQLPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            29422..30867
                     /gene="nuoN"
                     /locus_tag="WGR46_000437"
     CDS             29422..30867
                     /gene="nuoN"
                     /locus_tag="WGR46_000437"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0855445.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSSFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            30979..31278
                     /locus_tag="WGR46_000438"
     CDS             30979..31278
                     /locus_tag="WGR46_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0855446.1"
                     /translation="MTASMYILLVLALIFANVPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRVGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(31387..32283)
                     /locus_tag="WGR46_000439"
     CDS             complement(31387..32283)
                     /locus_tag="WGR46_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0855447.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTAILGKTAGKDADNDKPTYVRLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(32267..32491)
                     /locus_tag="WGR46_000440"
     CDS             complement(32267..32491)
                     /locus_tag="WGR46_000440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0855448.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(32489..32578)
                     /locus_tag="WGR46_000441"
                     /pseudo
     CDS             complement(32489..32578)
                     /locus_tag="WGR46_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(32605..33528)
                     /gene="rsgA"
                     /locus_tag="WGR46_000442"
     CDS             complement(32605..33528)
                     /gene="rsgA"
                     /locus_tag="WGR46_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0855449.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETHALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(33533..35389)
                     /locus_tag="WGR46_000443"
     CDS             complement(33533..35389)
                     /locus_tag="WGR46_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855450.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPTELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(35415..35552)
                     /locus_tag="WGR46_000444"
     CDS             complement(35415..35552)
                     /locus_tag="WGR46_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855451.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(35552..36136)
                     /gene="ruvA"
                     /locus_tag="WGR46_000445"
     CDS             complement(35552..36136)
                     /gene="ruvA"
                     /locus_tag="WGR46_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0855452.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(36218..36544)
                     /locus_tag="WGR46_000446"
     CDS             complement(36218..36544)
                     /locus_tag="WGR46_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0855453.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(36598..37317)
                     /locus_tag="WGR46_000447"
     CDS             complement(36598..37317)
                     /locus_tag="WGR46_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0855454.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVGQGTAHVKIEQIVPGQSTPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            complement(37314..37436)
                     /locus_tag="WGR46_000448"
     CDS             complement(37314..37436)
                     /locus_tag="WGR46_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0855455.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(37447..37911)
                     /gene="trmL"
                     /locus_tag="WGR46_000449"
     CDS             complement(37447..37911)
                     /gene="trmL"
                     /locus_tag="WGR46_000449"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0855456.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(37976..38473)
                     /locus_tag="WGR46_000450"
     CDS             complement(37976..38473)
                     /locus_tag="WGR46_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0855457.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLTD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            38529..39443
                     /gene="bioH"
                     /locus_tag="WGR46_000451"
     CDS             38529..39443
                     /gene="bioH"
                     /locus_tag="WGR46_000451"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0855458.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            39445..40233
                     /locus_tag="WGR46_000452"
     CDS             39445..40233
                     /locus_tag="WGR46_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0855459.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            40510..>40902
                     /locus_tag="WGR46_000453"
     CDS             40510..>40902
                     /locus_tag="WGR46_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ0855460.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTAL"
BASE COUNT         9487 a        10261 c        10943 g        10211 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatatgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg
     4921 cttttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc
     5101 ggtttgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc
     5521 ggcgacgacg accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg
     5581 ctgatggaag agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc
     6001 ctcttgcgcg tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt
     6061 cagacggcct caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca
     6121 aaagccacca aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac
     6241 tacaaaaccc aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca
     6361 gacgaccccg cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc
     6481 ttggaattta acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcacagcaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gaaatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt
     6961 gcgaaattgg aagcgatgta gaagggagat ggcagaggcc gtctgaaaac gggaacaaca
     7021 atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 gggaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactacaaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 aaagatcatg tgcaaatttg cgaggtatcg ggaatcggtt ttgaaaaaga aggaagcctg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg agtatccgcc catcggtgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccctatttg ccttagacta tcgggagtgt
     7621 ggaagagacg gagagccgaa agtggtgttt gtcgaacaag aatcggattt ttaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatt cacagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accagaatca tcaaaggaaa acgggcggaa
     7801 taacagataa ccgattgaat ccgtaaggaa aacggtttgt aggaacgtgc catccaagac
     7861 ctttgcaaag aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt
     7921 ggagctatct caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat
     7981 taaatgttgt tctagctctt gcactgattg aggattgcga attcctgata caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata aatagggaaa ttcaaattaa gttagaatta tccctatgag
     8701 aaaaagtcgt ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgcaggtgt
     8761 aactgcaaga acagcaacag agcctgacag cattgtttat acggattgtt atcgtagcta
     8821 tgatgtatta gatgtgagcg aatttagcca tttccgtatc aatcacagca cacattttgc
     8881 tgaacgacaa aaccatatca atggaattga gaatttttgg aaccgggcaa aacgtcattt
     8941 acgcaagttt aacggcattc ccaaagcgca ttttgagctg tatttaaagg agtgcgaatg
     9001 gcgttttaac aacagtgaga taaaagttct tgttccattt taaaacaatt agtaaaatcg
     9061 agtttatctt agttgtccag gacagcccca ttatttttat aacaccgtga agccgcacag
     9121 cagtttgaac agtgatacgc cgtttgcggg cttacgagtt tattttcccg gcctgcagtt
     9181 tgagcaatac ggtgatttcc tacggttaat acaaatgttt acacattgat acatttcatt
     9241 tatagttccg cctatttgaa aatagaaaat atgaattcga ccgcaagtaa aaccctgaaa
     9301 ggattgtcgc tggtgttttt cgcctctgga ttctgcgccc tgatttacta ggtcagctgg
     9361 cagaggcttc tattcagtca cataggtatc gatttaagtt cgattactgt cattatttct
     9421 gtatttatgg tcggcttggg tgtaggtgcg tatttcggtg gacgcattgc tgaccgtttt
     9481 ccttcaagta tcatccccct gttttgcatc gctgaagtat ccatcggtct gttcggtttg
     9541 gtaagcaggg gtctgatttc cggcttgggg catcttttag ttgaggctga tttgcccatc
     9601 atcgctgctg ccaatttcct cttattgctg cttcctacct ttatgatggg cgcgaccttg
     9661 cccttgctga cctgtttttt taaccggaaa atacataatg ttggcgagtc tatcggtacc
     9721 ttatattttt tcaacacttt gggtgcggca ctcggatcgc ttgccgccgc cgaatttttc
     9781 tacgtctttt ttaccctctc ccaaaccatt gcgctgacag cctgctttaa ccttctgatt
     9841 gctgcttcag tatggctgcg ttacagaaag gatggatata gtgaacacta aaccgaatac
     9901 tagtttgatt tatatgcttt ctttccttag cggcttattg agcttgggta tagaagtctt
     9961 gtgggtaagg atgttttcgt tcgcagcaca gtccgtgcct caggcatttt catttactct
    10021 tgcctgtttt ctgaccggta tcgccatcgg cgcgtatttt ggcaaacgga tttgccgcag
    10081 ccgctttgtt gatattccct ttatcgggca gtgcttcttg tgggcgggta ttgccgactt
    10141 tttgattttg ggtgctgcgt ggttgttgac gggtttttcc ggcttcgtcc accacgccgg
    10201 tatcttcatt accctgtctg ccgtcgtcag agggttgatt ttcccgctcg tacaccatgt
    10261 gggtacggat ggcaacaaat ccggacgaca ggtttccaat gtttatttcg ccaacgttgc
    10321 cggcagtgca ttgggtccgg tccttatcgg ctttgtgata cttgatttct tgtccaccca
    10381 acagatttac ctgctcatct gtttgatttc tgctgctgtc cctttgtttt gtacactgtt
    10441 ccaaaaaagt ctccgactga atgcagtgtc ggtagcagtt tccctaatgt tcggcatcct
    10501 catgttccta ctgccggatt ctgtctttca aaatattgct gaccgtccgg ataggctgat
    10561 tgaaaacaaa cacggcattg ttgcggttta ccatagagat ggtgataagg ttgtttatgg
    10621 ggcgaatgta tacgacggcg catacaatac cgatgtattc aatagtgtca acggcatcga
    10681 acgtgcctat ctgctaccct ccctgaagtt tggcatacgc cgcattttcg tcgttggatt
    10741 gagtacaggt tcgtgggcgc gcgtcttgtc tgccattccg gaaatgcagt cgatgattgt
    10801 tgcggaaatc aatccggcat accgtagcct tatcgcggac gagccgcaaa tcgccccgct
    10861 tttgcaggac aaacgtgttg aaattgtatt ggatgacggt aggaaatggc tgcgtcgcca
    10921 tcctgatgaa aaattcgacc tgattttgat gaatacgact tggtactggc gtgcctattc
    10981 caccaacctg ttgagtgcgg aatttttaaa acaggtgcaa agccacctta ccccggatgg
    11041 tattgtaatg tttaatacca cgcacagccc gcatgctttt gctaccgccg tacacagtat
    11101 tccctatgca taccgctatg ggcatatggt agtcggctcg gcaaccccgg tagttttccc
    11161 taataaagaa ctgctcaagc aacgtctctc ccggttgatt tggccggaaa gcggcaggca
    11221 cgtatttgac agcagcaccg tggatgctgc agcacaaaag gttgtctctc gtatgctgat
    11281 tcagatgacg gaaccttcgg ctggggcgga agtcattacc gacgataata tgattgtaga
    11341 atacaaatac ggcagaggga tttaaccgtc ttaaagggtt tcaggcaacg caggttttag
    11401 gtaacgtcct gctagttcaa aaaaaccgca tcacagcagt cgggacaaaa tggtttaaac
    11461 attttgtccc gaattcttat tcctatatat agtggattaa caaaaatcag gacaaggcga
    11521 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag
    11581 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
    11641 taccacgaat tacggtgtaa aaatttatat gaccttataa aatcaaataa gaatcgttat
    11701 cataacatga ttgtatttat tgggtttttt tgggcgtttt gccgatattt accttttaat
    11761 ggtttttgaa attcgctaaa atacgaaatt attgtagaaa ttttgttaac ggatttgggt
    11821 gtaaccatgt tgtccgctta ctttcccgtc tttgtcttta tcctcgtcgg cctcgcggcc
    11881 ggcgtgctgt ttatcctgct cggcacgatt ttgggcccga aacgccacta cgccgaaaaa
    11941 gacgcgcctt acgaatgcgg ttttgaggcc tttgaaaacg ccaggatgaa gttcgacgtg
    12001 cgctattacc tcgtcgccat cctctttatc ctgtttgatt tggaggtcgc gttcatgttg
    12061 ccgtgggcag ttgtgttcaa agatttgggt gcgtacggct tctggtctat gctggtgttt
    12121 atcgtcgttc tgacggtagg ctttgtttac gaatggaaaa aaggtgcgct ggaatgggaa
    12181 tagaaggcgt tttgaaaaaa ggtttcatca ccaccagcgc ggatacggtg ttgaactata
    12241 tgcgtaccgg ttcattgtgg ccggttactt tcggcttggc ctgctgcgcc gtggaaatga
    12301 tgcatgcggg tatggcgcgt tatgaccttg accgcttcgg catcattttc cgtccgtccc
    12361 cacgtcaggc cgacctgatg attgtggcag gtacgctgac caacaaaatg gcgcccgccc
    12421 tgcgccgcgt atacgaccag ctcgccgagc cgcgctgggt attgtctatg ggctcgtgtg
    12481 ccaacggcgg cggctattat cactattctt attccgttgt gcgcggtgcc gaccgcgtcg
    12541 tgccggtaga cgtttatgtg ccgggttgtc cgccgactgc ggaagccctg atttacggtc
    12601 tgattcagct ccaacaaaaa atcaagcgca cttccaccat cgcgcgtgac gagtaaggag
    12661 aggacgatat ggcaagcatt caaaacttat acgaaaccgt cagccgcgtt ttgggcaatc
    12721 aggcaggcaa agtcatttcc gctttgggcg agattaccgt cgagtgtctg cccgaacact
    12781 atatttcagt catgaccgca ttgcgcgacc atgaagagtt gcatttcgag cttctggtcg
    12841 acttgtgcgg tgtcgattac agcacttaca aaaacgaagt atggcagggc aaacgctttg
    12901 ccgtcgtcag tcagttgctt tccgttaaaa acaatcaacg catccgcgta cgcgtctggg
    12961 tttcagacga cgacttccct gtagtcgaat ccgtagtcga tatttacaac agcgcggatt
    13021 ggtacgaacg cgaagccttc gatatgtacg gcatcatgtt caacaaccac ccggacttgc
    13081 gccgcatcct gaccgattac ggctttgtcg gacatccgtt ccgcaaagac ttcccgattt
    13141 ccggctatgt ggaaatgcgt tacgacgaag agcaaaaacg cgtgatttac caacctgtta
    13201 ccattgagcc gcgcgagatc acgccgcgta tcgtccgtga ggagaactac ggtggccaat
    13261 aaattaagaa actacaccat caacttcggc ccgcaacacc ctgcggcgca cggcgtattg
    13321 cgtatgattt tggagctgga cggcgaacaa atcgtccgtg ccgacccgca tatcggcctc
    13381 ctgcaccgag gcacagagaa actggcggaa accaaaacct acctgcaagc cctgccttat
    13441 atggacaggc tggactacgt ttccatgatg gtcaacgagc aggcgtattg cttggcggta
    13501 gaaaaacttg ccggtatcga tgtgcctatc cgcgcccaat acatccgcgt gatgtttgcc
    13561 gaagtaacgc gcatcctcaa tcacttgatg ggcatcggtt cgcatgcctt cgacatcggc
    13621 gcgatgaccg ccattcttta cgccttccgc gaccgtgaag agctgatgga cttgtacgaa
    13681 gccgtttccg gcgcgcgtat gcacgccgcc tacttccgtc ccggcggcgt ttaccgcgac
    13741 ctgcccgact tcatgcctaa atacgagggc agcaaattcc gcaatgccaa agtattgaag
    13801 cagctcaacg aatcccgcga aggcacgatg ctcgacttta tcgatgtctt ctgcgaacgt
    13861 ttcccgaaaa atatcgacac actcgaaacc ctcctgaccg acaaccgtat ttggaaacag
    13921 cgtaccgtcg gcatcggcgt cgtctccccc gaacgtgcca tgcaaaaagg ctttaccggc
    13981 gtgatgttgc gcggttcggg tgtggaatgg gacgtgcgta agaaacagcc ttacgaagtg
    14041 tacgacaaaa tggatttcga catccctgtc ggcgtgaacg gcgactgcta cgaccgctac
    14101 ctctgccgta tggaagaaat gcgccaatcc gtacgcatca tcaaacaatg ctccgagtgg
    14161 ttgcgtgtca atccgggtcc ggtcattacc acaaaccaca aattcgctcc gcccaaacgt
    14221 accgaaatga aaacaggtat ggaagacctg attcaccatt tcaaactctt taccgagggt
    14281 atgcacgttc ccgagggcga gacttacacc gctgtcgaac atccgaaagg cgagttcggc
    14341 gtttacatca tttcagacgg cgcgaacaaa ccctaccgcc tgaaaatccg cgcacccggc
    14401 ttcgcccatc tgcaaggtat ggacgaaatg gcaaaaggcc atatgctcgc cgacgtcgtt
    14461 gccatcatcg gtacgcagga catcgtattc ggggaggttg accgataatg ttatccgcag
    14521 aatccttaaa acaaatcgac atcgagttgg cgaaatatcc cgccgaccaa cgccgctctg
    14581 cgattatggg cgcattgcgt attgcccaga ccgaaaaagg ctggctcgcc cccgaaacca
    14641 tcgcttttat cgccgactac atcggcatct cgcctgcaca agcctacgaa gtcgctacct
    14701 tctacaatat gtacgacctt gagcctgtcg gcaaatacaa actgaccgtt tgtaccaacc
    14761 tgccctgcgc cctacgcggc ggtatggcga ccggcgaata cctcaaacaa agactcggta
    14821 tcggctacgg cgaaaccacg cccgacggca aatttaccct tgtcgaaggc gaatgcatgg
    14881 gcgcgtgcgg cgacgctccg gttatgctgg tcaacaacca cagcatgtgc agctttatga
    14941 ccgaagaggc gattgagaag aaactggcgg agttggagta ggtcgtctga aacgacgatt
    15001 taaacgtagg tcggatactt gtatccgaca gagtgggtaa aaaggcaaaa tgtcggattt
    15061 aagaatccgc cctactgaaa taccgaaatg ccgtcattcc cgcgcaggcg ggaatccacc
    15121 ggtaagattc ggtttctgaa tttaataaga cattgcttac cattgaggat ggattcccgc
    15181 ctgcgcggga atgacgacag acaagcaagt ggtcgagatc caacaaaaac gattaaaggt
    15241 cgtctgaaaa tatcgatttg ataaactaga ttttatttca gacgacgtta caagccggta
    15301 cacaaagaca tcttaaggtc gtctgaaaca gcggccgcaa ccgatacgaa aacaaacagg
    15361 cacaccaaaa atggctattt accaatcagg cgtgattttt gaccaagtgg ataccgccaa
    15421 tcccgactgc tggacattgg acgaatacgt caaacgcggc ggctataccg ccctgcgtaa
    15481 aattctgtcc gaaaacatct cgcaaaccga tgtgattgac gaagtcaaaa cctccggctt
    15541 gcgcgggcgc ggcggtgcgg gcttcccgac tggtttgaaa tggagcttta tgccccgttc
    15601 cttcccgggc gaaaaatatg tggtttgtaa caccgacgaa ggcgaaccgg gtacatttaa
    15661 agaccgtgac atcatcatgt tcaatcctca tgccctgatt gaaggcatga ttatcgccgg
    15721 ttacgcgatg ggtgcgaaag ccggctacaa ctatatccac ggcgaaatct tcgaaggcta
    15781 ccaacgcttt gaagccgctt tggagcaggc gcgtgccgca ggctttttgg gtaaaaatat
    15841 tttgggttcg gattttgaat ttgaactctt cgcccaccac ggctacggcg catatatttg
    15901 cggcgaagaa accgcattgc tcgaatcgtt ggaaggcaaa aaaggccagc cgcgcttcaa
    15961 accgccattc cctgcttcgt tcggcctgta cggcaaaccg accaccatta ataatactga
    16021 aaccttttcc tccgtcccat tcattgtccg tgatggcgga caggcatttg ccgataaagg
    16081 tattccgaat gcaggcggta ccaaattgtt ctgtatttcc ggccatgtcg agcgtccggg
    16141 caactatgaa gtgccattgg gcacaccgtt tgccgaagtc ttgaaaatgg caggcggtat
    16201 gcgcggcggc aaaaaactca aggccgttat tcccggcggt tcgtccgcgc ccgtgttgcc
    16261 tgccgatatc atgatgcaga ccaatatgga ctatgactcg atttctaaag caggctcgat
    16321 gttgggttcc ggcgcgatta tcgtcatgga cgaagatgtg tgcatggtca aagcccttga
    16381 acgcctgagc tacttctact atgacgagtc ttgcggccaa tgtacgcctt gccgagaagg
    16441 tacgggctgg ctctaccgca tcgtccaccg catcgtagaa ggcaaaggca aaatggaaga
    16501 tttggatttg ctggattccg tcggcaacca aatggcaggc cgcaccatct gcgcccttgc
    16561 cgatgccgcc gttttccctg ttcgcagctt taccaagcat ttccgtgatg agtttgtgca
    16621 ttacatcgaa cacggcggac cgatgaaaga gcataagtgg ggagggtggt aatggtggaa
    16681 gctaaaattt ttattctata cggtgcagcc aacaaaggta agagtacgac actcaatacg
    16741 ctttttaatc agatttgttg gaaattttct aaatttctag tcttttttga aagacatgga
    16801 aacggcttag attttgttgc agtatttgat catgaaggtc agaggattgg tttttattca
    16861 tctggtgata atgaatacga ggttaggaga aatttataca aactttattc gcataattgt
    16921 gattttattt ttggcacgtc aaggacacgg ggtggtagtt gcgatgcagt aggatgttat
    16981 gcagagttat tgcatggcga tgtaaatata attaattggt gtgaaaagtt tgagcctaca
    17041 gatgaagaca atgagcgtgc tgttaaagag ttatttaagt catttaaaaa tataataaat
    17101 gagttatagt tttagttggt tttatattgg ttaaaagcaa aatgctaaaa atttaacttt
    17161 gccgtcattc ccgcgtaggc gggaatccat agtggaattt acagaacccg atatttgaaa
    17221 agcagttacc gaaaacccaa gaatggattc ccgcctgtgc gggaatgacg gcaaggtggc
    17281 agtaaacgtt ttaaacagta ttgattgtca atgaaactca aaaggccgtc tgaaacccat
    17341 ttttcagacg atctccataa agaattattt atcaaatacc cgtaactagg aacgaaccat
    17401 gttacaaatc gaaatcgacg gcaaacaagt atctgtggag cagggcgcga cggtgattga
    17461 agccgcgcac aagctcggta cttatatccc gcatttctgt taccacaaaa aactttccat
    17521 cgccgccaac tgccgtatgt gtttggtgga cgtggaaaaa gcccccaaac ctctgccagc
    17581 ctgtgccacg ccggtaacgg acggcatgat tgtgcgtacg cattcggcaa aagcccgaga
    17641 ggcgcaagaa ggcgtgatgg agttcctgct catcaaccat ccgcttgatt gtccgacctg
    17701 cgaccagggc ggcgaatgcc agttgcagga tttggcgatg ggctacggca aaaccaccag
    17761 ccgttatacc gaagaaaaac gttccgtcgt cggcaaagat atgggacctt tggtttccgc
    17821 cgaggaaatg agccgctgca tccattgcac ccgctgcgtg cgattcaccg aagaaattgc
    17881 cggtttgcag gaaatcgcga tggcgaatcg cggcgaacac tccgaaatca tgccttttat
    17941 cggcaaagca gtggaaaccg aattgtcagg caacgtcatc gatttgtgtc ctgtcggtgc
    18001 attgaccagc aaaccgttcc gcttcaacgc gcgtacttgg gagctgaacc gccgcaaatc
    18061 cgtttctgcc catgatgctt tgggcagcaa tttgattgtg caaaccaaag accataccgt
    18121 ccgccgcgtg ctgccgttgg aaaacgaagc gattaacgaa tgctggctgt ccgaccgcga
    18181 ccgtttcgcc tacgaaggcc tgtatcacga aagccgtctg aaaaacccga aaatcaaaca
    18241 gggcggcgag tggatggacg tggattggaa aaccgcgttg gaatatgtcc gcagcgcgat
    18301 tgaatgtatc gccaaagacg gtaaccaaaa ccaagtcggc atttgggcga acccgatgaa
    18361 tacggttgaa gagctgtatc tggcgaaaaa actcgccgac ggcttgggtg ttaaaaactt
    18421 tgccacccgt ttgcgccaac aagacaaacg tctttcagac ggcattaaag gtgcgcaatg
    18481 gttgggacaa agcattgaat ctttggctga caacgatgcc gtattggtag tcggtgcgaa
    18541 cttgcgcaaa gaacagccgc tcctgaccgc ccgcctgcgc cgcgccgcca aagaccgtat
    18601 ggctttgagc gtattggccg gcagtaaaga agaattgttt atgccgcttc tgtctcaaga
    18661 agccgcacat cccgacgagt gggcagaccg tctgaaaaaa ctgtctgcca atgcggaaca
    18721 cgccgttacc gccagcctga aaaacgctga aaaagcagcg gtgattttgg gcgcggaagt
    18781 gcaaaaccat cctgattacg ccgccattta tgccgccgcg caagagctgg cggacgcgac
    18841 cggcgcagtg ctgggtattt tgccgcaagc cgccaacagt gttggtgcgg atgtcttgaa
    18901 tgtaaactcc ggcgagagcg ttgccgaaat ggcaaacgcg ccgaaacagg cagtcttgtt
    18961 gctcaacgtc gagccggaaa ttgatacggt tgacggtgca aaagccgtag ccgcgttgaa
    19021 acaggcaaaa agcgtgatgg cgtttacgcc gtttgtcagc gaaacgctgc tggacgtgtg
    19081 cgacgtgttg ttgccgattg cgccgtttac cgaaacctca ggcagcttca tcaatatgga
    19141 aggccgtctg caatctttcc acggcgtggt acaaggcttc ggcgattcgc gtccgctgtg
    19201 gaaagtgttg cgcgtattgg gcaacctgtt tgacctgaaa ggttttgaat accacgatac
    19261 cgctgcgatc ttgaaagacg cgctggatgc agaaagcctg ccgtccaaac tggacaaccg
    19321 cagtacatgg acaggggagg gcgttcagac ggcatcagac cgcctcgtgc gcgtgggcgg
    19381 agtgggcatc tatcacaccg atgccatcgt gcgccgttcc gcaccgttgc aggcaaccgt
    19441 ccatgccgcc gtgcctgccg cgcgtgtgaa tccgaacact ttggcgcgct tgggcctgca
    19501 agacggacaa accgctgttg ccaaacaaaa cggcgcaagc gtatcggttg ccgtcaaagc
    19561 cgatgccggt ttgcctgaaa acgtggtgca tctgtcgctg cataccgaaa atgccgcgct
    19621 gggtgcgttg atggacacta ttgaactggc gggagcttga tcatgcaaga atggttccaa
    19681 aacctctttg ccgcaacgct cggtctgggc gatttgggta tcaccgtggg cttggtggta
    19741 tccgtcatcg tcaaaatcgt gattatcctg attccgctga ttctgaccgt cgcctacctg
    19801 acttatttcg aacgtaaagt catcggcttc atgcagcttc gcgtcggccc gaacgtaacc
    19861 ggcccgtggg gcctgatcca gccgtttgcc gatgtgttca aactcttgtt taaagaggta
    19921 acccgtccga agctgtcaaa caaagccctg ttctatatcg gcccgattat gtcgcttgcc
    19981 ccgtctttcg cggcgtgggc agtgattccg ttcaacgaag aatgggtgct gaccaatatc
    20041 aatatcggcc ttttgtacat cctgatgatt acctcgctgt cggtttacgg cgtgatcatc
    20101 gcgggctggg cttccaactc aaaatattcg ttcttgggcg caatgcgtgc ttccgcgcaa
    20161 agcatctcct acgaaatcgc catgagcgcc gcgctggtgt gcgtcgtgat ggtgtcgggc
    20221 agcatgaact tctccgacat cgttgccgca caagccaaag gcatcgcggg cggttccgtg
    20281 ttctcgtgga actggctgcc gctcttcccc atcttcatcg tctatctgat ttccgccgtt
    20341 gccgaaacca accgcgcgcc gtttgacgtg gcagagggtg agtctgaaat cgttgccggc
    20401 caccacgtcg aatactccgg cttcgcattc gcgctgttct tccttgccga atacattttc
    20461 atgattctga tttccgcgct gacatcgttg atgttcctcg gtggttggtt gtctccgttc
    20521 ccacaaagct ggggctttat cggcacgcct tccgcattct ggatgttcgt gaaaatggcg
    20581 gcggtgttgt actggtatct gtggatccgc gcaactttcc cacgctaccg ttacgaccaa
    20641 atcatgcgtt tgggctggaa agtgctgatt ccgatcggct tcgcctacat cgtgattttg
    20701 ggtctgtgga tgatttcacc gctgaatttg tggaaataag tttcagacga cgtatcaagg
    20761 tcgtctgaaa acgcagtttt gaatatttaa cagaatccct gttttgaggg aacataatat
    20821 ggctaactta gtaaaaacct ttctgcttgg cgaattggta aaaggtatgg gcgtaacgct
    20881 caaaaacttt tttgcccgca aagacacgat ttattttccc gaagagaaaa cgccgcaatc
    20941 cgtgcgtttc cgcggtctgc acgcgcaacg ccgttatcca aacggcgagg aacgctgcat
    21001 cgcgtgtaaa ttgtgtgaag ccgtttgccc cgcaatggcg atcaacatcg aatcggaaga
    21061 acgcgaagac ggcacgcgcc gcaccaagcg ttacgacatc gacctgacca agtgcatctt
    21121 ctgcggtttc tgcgaagaag cctgcccgac cgatgcgatt gtggaaaccc atatttttga
    21181 ataccacggc gagaaaaaag gcgacttgca catgaccaag ccgattcttt tggccattgg
    21241 cgacaaatac gaagctgaaa tcgccaaacg caaagccgct gacgcgccgt atcgttaatg
    21301 ctttggggct tcttggaagg ttttaaatat ggaaggactg attaatgcat tgaaatattt
    21361 agccgaacat gagccaatag ataattttga agaaattaga actagaaata gtccgattga
    21421 gttgccaagt ggattaagta attttgaaca aaatattttt ttaaaagaaa atttatcccc
    21481 aaaattacaa aatgatgata gcttgaagac gcattattgg attatccgtg aatggggtgg
    21541 gattaaaagt tttaaacaat ctgctgaaaa tagccagctt attcgtcaat ttttatcgga
    21601 acttaattcg ggaaaattga gtagtggttt gttgaaaatt tcatcattat ctaaattggc
    21661 ttcttttata gattgtgagc gattcgccat ttatgattca cgcgctattt tttcgttgaa
    21721 ttggttgttg tttaaattta caaatgcaga tttgtttttt cagccacaag gtagaaatag
    21781 ggaactagaa atccgaaata tgaacgtatt gtttcatttt tctgatatca aaccgaatta
    21841 tcggaaacca gacgtttcgt ttcatcaata ttgtggattg ttacaagatt tggcgaaaca
    21901 agtttatggt aaacaagcaa aaccgtatca catagaaatg ttgttattca aaattgcgac
    21961 aacgtggatt tgtgcggata tggatcaact gattaagttt gattgtttgc gtaaccagga
    22021 ttttcagact gcttgaaacc atatttttga ttaataaaga aagcatagac tatgactttc
    22081 caactgattt tattttatat ttttgcagtg ataattcttt atggcgcgat caaaaccgtc
    22141 accgctaaaa atcctgttca cgccgctttg catctggtgt tgaccttctg cgtgagcgcg
    22201 atgctttgga tgctgatgca ggctgagttt ttgggcgtga cgctggtggt ggtttacgtc
    22261 ggcgccgtga tggtgttgtt cctgttcgtc gtgatgatgt tgaacatcga cattgaagaa
    22321 atgcgtgccg gtttctggcg gcacgcgcct gttgccggtg tggtcggcac attgttggca
    22381 gttgccctga ttctgattct ggtcaatccg aaaaccgacc ttgccgcatt tgggctgatg
    22441 aaagacattc ccgccgatta caacaatatc cgcgatttgg gcagccgtat ttataccgat
    22501 tatctgctgc cgtttgaatt ggcggcggta ttgctgctgt tgggtatggt ggccgcgatt
    22561 gcgctggttc accgtaaaac ggttaatccg aaacgtatgg atcctgccga ccaagttaaa
    22621 gtacgcgccg accaaggccg tatgcgcttg gtgaaaatgg aagccgtgaa accgcaaacc
    22681 gaatctgccg aagaaagcga agtttcagac gacctcaagc cgaaagagga gggcaaagca
    22741 tgattacctt gacgcattat ctggtattgg gtgcgctcct gttcggtatc agcgcgatgg
    22801 gtatctttat gaaccgcaaa aacgtgctgg tcttgctgat gtccatcgag ctgatgcttt
    22861 tggcagtgaa cttcaacttt atcgcctttt cgcaacattt gggcgatact gccggacaaa
    22921 ttttcgtatt tttcgtactg accgttgctg ctgccgaatc tgccatcggt ttggcgatta
    22981 tggtgctggt gtaccgcaac cgacaaacga ttaacgttgc cgatttggac gagttgaaag
    23041 ggtaaaggta ggttgggtcg agacctgaca agacaccgat gccgtctgaa aacccgatag
    23101 gaaaaacgat gaaatccata gacgaacaaa gcctgcataa tgcccgccgc ctgtttgaaa
    23161 gcggcgacat cgaccgtatc gaagtcggta ccaccgcggg cctgcaacag attcaccgtt
    23221 acctgttcgg cggcttatat gattttgcgg gtcaaatcag ggaagacaac atttccaaag
    23281 gcggttttcg ttttgccaac gccatgtatt taaaagaggc tttggttaaa atcgagcaga
    23341 tgcccgagcg gacttttgaa gaaatcatcg ccaaatatgt tgaaatgaac attgcccatc
    23401 cgtttttgga gggtaatggc agaagtaccc gcatctggct ggatttggtg ctgaagaaaa
    23461 acctgaaaaa agtcgtgaac tggcaaaatg taagcaaaac cctgtatttg caggcgatgg
    23521 aacgcagccc cgtcaacgat ttagaactgc gctttttgtt aaaggacaac ctgaccgacg
    23581 atgtggacaa ccgtgaaatc atctttaaag gtatcgagca gtcgtattat tacgaagggt
    23641 atgaaaaagg ctgaaggtcg tctgaaaagc gatttcagac tgtttcagac gacctgattt
    23701 ggtaggtgat cagacgggag cggatgagaa aagaaattct aggtaagaat aatccggtct
    23761 gaaatattgg aagaagaatg atggataaaa atcagttaga acaagaattt cataaagcca
    23821 tgttaaatat ttatcaggag gctttgaatt tgccgcaacc ttacaaggcg acacgatttt
    23881 tacaaattgt aaatgaattt ggtggtaaag aggcggcgga taaattatta agtacggggg
    23941 aaaagaagac tcagaccggt tttacagagc tgattttgag tggtggcgga gtccacgcct
    24001 tgaaatacag tatggaatat ctggtgttac aaaagccgtg gtgtgattta tttactgaag
    24061 agcaattagc tgtggcacgc aaacgattgg agcgtgttgg atttgtttct ccgaagtaat
    24121 tttgtacgaa acaaacatag atttttaaat caatcggatt caatcaaatg aacgacatga
    24181 ctttatatct catcatcgcc ctcgtgcctt tggcaggctc gctgattgcg ggtttattcg
    24241 gcaacaaaat cggacgtgcc ggcgcgcata cggttacgat actcggtgtg gcggtgtccg
    24301 ccgtgctgtc ggcttatgtg ctgtggggat tcctcaatgg cagccgtgcc aagtttgacg
    24361 agaacgtcta tacctggctg acaatgggcg gtttggattt ctccgtcggc ttcttggtcg
    24421 atacgatgac ggcgatgatg atggtcgtgg taacgggcgt gtcgttgatg gtgcatatct
    24481 acaccatcgg ctatatgcac gatgaaaaag tcggctacca acgcttcttc agctatattt
    24541 ctttgtttac tttcagtatg ttgatgctga ttatgagcaa caacttcatc cagctcttct
    24601 tcggctggga ggctgtgggc ttggtatcgt atctcttgat cggtttctat ttcaaacgcc
    24661 cgagcgcaac atttgccaac ctgaaagcct ttttgatcaa ccgtgtcggt gacttcggct
    24721 ttttgctcgg tatcggcttg gtgcttgcct atttcggcgg cagcctgcgc tatcaagatg
    24781 tattcgctta tctgcccaac gtccaaaatg ccaccatcca gcttttcccc ggtgtggaat
    24841 ggtctttgat taccgtaact tgtttacttc tgtttgtcgg tgcgatgggt aaatcggcac
    24901 aattcccgct gcatgtctgg ctgcccgatt cgatggaagg cccgaccccg atttccgcat
    24961 tgattcacgc cgcaaccatg gttaccgccg gtctgttcat ggtatcgcgt atgtcgccga
    25021 tttatgagat gagtagcact gcgctgtctg tcatcatggt gattggtgcg attaccgccc
    25081 tgtttatggg tttcttgggc gtgattcaaa acgacatcaa acgtgtggtt gcgtattcca
    25141 ccctgtcgca actgggctat atgaccgtgg ctctgggcgc gtctgcctat tccgtggcga
    25201 tgttccatgt gatgacccac gctttcttta aagccttgct gttcttggcg gcgggcagcg
    25261 cgattatcgg tatgcaccac gaccaagata tgcgccacat gggtaacctg aaaaaataca
    25321 tgccgattac ttggctgact atgctgatcg gcaatttgtc gctgatcggt acgccgttct
    25381 tctccggctt ctactccaaa gattcgatta tcgaagcggc gaaatacagc accctgccgg
    25441 gcagcggctt tgcctatttc gccgtcctcg ccagcgtgtt tgttactgcg ttctacgcat
    25501 tccgccaata ctttatggtg ttccacggcg aagagaaatg gcgcagcttg cctgaacatc
    25561 atgacgatca tcatggcgaa gaacatcaca gcttgggcaa aaacgacaat ccgcacgaaa
    25621 gcccgttggt tgttaccctg cctttgattt tgcttgccat tccgtccgtc atcatcggct
    25681 acgttgccat cgagcctatg ctttacggcg atttctttaa agacgtgatt ttcgtcaata
    25741 ccgacgcgca tccgaccatg cacatcatga aggaagagtt ccacggcgca ttggcaatgg
    25801 tgtcgcacag cttgacatcg cctgttttgt atttggcggc tgccggtgtt gccgccgcat
    25861 ggcttttgta cgtcaaactg ccgcacctgc cagcgaaaat tgcacagacg ttccgtccga
    25921 tttacgtttt gtttgaaaac aaatactacc tcgacgccct gtatttcaac gttttcgcca
    25981 aaggcacacg cgcattgggt accttcttct ggaaagtcgg cgataccgcc attatcgaca
    26041 acggcatcgt caacggctct gccaaactgg tcggcgcgat tgccgcgcaa gtgcgtaaag
    26101 ctcaaaccgg ctttatctat acctacgccg ccgctatggt gttcggcgta ttggtactgc
    26161 tcggcatgac cttctggggc ttgttcagat aaaggccggc ttcagacgac ctgttcatgg
    26221 ttgtgtagag gtcgtctgaa aaaagaaact tgagaaacaa tgtcaaacac acaaaccatc
    26281 cgctctgccg acttcaccgc ttcccgcgca tggggtgcac tcgacatcgc caatatgaac
    26341 ggcaccaccg tgcgcctgca ctggacggac cagtcctaca aatggcacgt caacgacggc
    26401 gaggaagtgt ttgccgtgat gaacggcgaa gtcgatatgc actatcgaga aaacggcgaa
    26461 gaacacatcg ttcggttgaa aagcggcgac atcttctacg caggaattgg cacggaacac
    26521 gtcgcccatc cgtgcggcga agcgcggatt ttggtgattg agaaagaagg cagtgcttga
    26581 ggtcgtctga aaagagggtt tcaggctgca atggaattgt aggttgaggt aagaagagat
    26641 gacgcagtta tttttacatt cagatacacc aaatgagaag gaactgattt caatatatga
    26701 gaaagctgtt tcagaggcag tagagctgta tattttctct gcgtatctca ctcattggga
    26761 tgtaaaaaat aagcttaata gcaaattaca agagttcaaa tttatttttg ggcaggattt
    26821 cggattgtct aaaaaagagg ctattcagga agtattgaaa tggcttccgt cccatttgaa
    26881 atttacattg atggtagctc aaggcattca gggttttcat cctaaagctg tattttggaa
    26941 gaatgataag aatgaatatt atgctttaat cggttcttca aatttgactc atgctgcgtt
    27001 taatagcaat tatgaagcaa atattttgac taaaatttct gagcaggatt ttataaaagc
    27061 taaatcttgg gctaatgaaa ttgtgatgaa atctatcccg gtatcggaag attggcttga
    27121 ggagtatcag gaagctgaaa ttaactataa gaaatcaact gttagacaat cagtgatgga
    27181 taagttattt atggagatgc caaattataa tcaagaactg attgcggcac ggagaaagca
    27241 gatgagaaac catcaaactg tgtgtaatca attaaaaaat cttattaagc aatgtgcagc
    27301 aggaaaaata gacaataatg atttttatgg ggaatttaat aaattatggt cttggaaaag
    27361 cgaaaataaa ggagaagggg taggcaatcg ttttcaagat aaaacgtgga aaaggacggg
    27421 taagtcttca gattttagaa aattatgcat tgccatccaa tcagtttttg atgctccact
    27481 tactgagaga gataatgtgg ttgccaagca aattgattgg ctaaaagaat gtggggtttc
    27541 cactcgtggt tcagtttttt ctgaaatgct ttgtcaagaa tatccagata gatatcctgt
    27601 tttaaatgct cccattaaaa aatttttaga agaaaataaa tttaagtctg caaaaggagc
    27661 aagcgaaggc tccaaatata ttgatttaag tatgaagtta agagctttac tagctatgca
    27721 gtcagaaata aaagatttgg ctgaattaga tgttttagta caggcagaat atcgcaatcg
    27781 tacagatatt gattgggaat aagtaaagaa ggggggggag tattcattcc ttctttcaga
    27841 cgacttccta aaacgctgtc tgtaaagacc aacccataca actatatttt ttattttaac
    27901 cacaggttaa ccactatgtt ttccaactac ctactcagct tggcaatatg gatacccatc
    27961 gttgcgggcg tgctggtttt ggcgacgggg ccggacagcc gtgcgccgtt tgcccgcgtg
    28021 ctcgctttaa tcggtgcgct tgccggtttc ttggtaacgc tgcccctgtt taccggtttc
    28081 gaccgtttga gcggcggcta tcagtttacc gagttccacg agtggattcc gcttctgaaa
    28141 atcaactacg cattgggcgt ggacggtatt tcagtgctct ttatcatctt gaatgcgttt
    28201 attacgctgt tggtggtatt ggccggttgg gaagtcattc agaagcgtcc ggcgcagtat
    28261 atggcggcat tcctgatgat gtcgggtttg attaacggtg cgtttgccgc gcaggatgcg
    28321 attctgtttt atgtgttctt cgagggtatg ctgattccgc tgtacctgat tatcggtgta
    28381 tggggcggcc cgcgccgcgt ctatgcgtcg gtcaagctgt tcctctatac gctgatgggt
    28441 tcgctcctga tgctggtggc gatggtttac ctttactatc aaacaggcag cttctctatt
    28501 gtcgatttcc aaaacatcaa acagcttccg ttgggcgtgc aacagctttt gtttgcggcg
    28561 ttcttcctgt catttgccgt gaaagtgccg atgttccccg tgcacacttg gctgccggat
    28621 gcccacgttg aagcaccgac cggcggttcg atggtgttgg cggcaattac gctgaaactg
    28681 ggtgcgtatg gtttcttgcg ctttatcctg ccgattatgc cggatgcggc tcgctatttt
    28741 gcccccgtga tcatcgtatt gagcctgatt gccgtaattt atatcggtat ggtggccctg
    28801 gtgcaaaccg atatgaaaaa actggtggcg tattcgtcca tcagccatat gggttttgta
    28861 acgcttggga tgtttttgtt tgttgacggg cagttggacg actgggcatt gaaaggtgca
    28921 atcattcaaa tgatttccca cggtttcgtg tctgccgcga tgtttatgtg tatcggcgtg
    28981 atgtacgacc gcctgcacac gcgcaatatt gccgattatg gcggcgtggt caatgtgatg
    29041 cccaagtttg cggcgtttat gatgctgttc ggtatggcga acgccggtct gcctgcgact
    29101 tccggcttcg tgggcgagtt tatggtgatt atgggcgcgg tcaaagtgaa tttctgggtc
    29161 ggcgcgctgg cggccatgac cctgatttac ggtgcatctt ataccctgtg gatgtacaaa
    29221 cgcgttattt ttggtgcgat ccacaatccg cacgttgccg aaatgcaaga catcaattgc
    29281 cgcgaatttg cgattttggc ggtgttggcg gttgctgttt tgggtatggg cctgtatccg
    29341 aacgcattta tcgaagtggt gcatcaggcg gcaaacgatt tgattgccca tgtggcacaa
    29401 agcaagattt gaggtgtgta aatgaattgg tctgatttga atttaatgcc cgcactgccc
    29461 gaagtcgcgc tgctgtcgct gctggtgtta ttgttgctgg cggacttgtg ggtcagtgat
    29521 gacaaacgcc ggtggacgca ttacggcgcg ttggcaacgg tggcggttac ggctgtggtg
    29581 cagcttgccg tttgggaaca gggcagcacg tcttcgttca acggcatgta tattgcagac
    29641 ggtatgtcgc gtttggcaaa aatggtttta tatgccttga cgtttgccct gtttgtctat
    29701 gccagaccct acaaccaagt gcgcggtatt ttcaaaggcg agttttacac cctgtcgctg
    29761 tttgccctct tgggcatgag cgtgatggtg agcgcggggc attttttaac tgcctatatc
    29821 ggtttggaac tcttgtcgct tgccctttac gccctgattg ccctgcgccg cgattccagc
    29881 tttgccgccg aagccgcctt gaaatatttt gttttgggcg cgctggcatc cggcctgctg
    29941 ctctacggta tttctatggt ttacggcgca accggttcgc tggaatttgc cggcgtgctc
    30001 gcctcttcct tcaatgaaga agcaaacgaa tggctgttga aactgggtct ggtgtttatc
    30061 gtcgtcgccg ttgcgttcaa actcggcgcg gtgccgttcc atatgtgggt gcccgacgtg
    30121 tatcacggcg cgcccacttc tgttaccgcc ctggtcggca ctgccccgaa aatcgccgcc
    30181 gttgttttcg ctttccgcat cctcgttacc gggttgggaa ccgtgcatca tgactggtct
    30241 ctgatgtttg ccctgcttgc cgccgcctcg ctgctggtcg gcaaccttgc cgccatcatg
    30301 cagaccaata tcaaacgtat gctcgcctat tccaccgtat cgcatatggg gtttatcctg
    30361 ctggcgttta tggcgggcgc ggtcggcttt gcggcgggtc tctattacgc cattacctac
    30421 gcgctgatgg cggcggcagg gttcggtgtg ttgatggtgt tgtcggacgg ggacaacgag
    30481 tgcgaaaaca tcagcgattt ggcagggttg aaccaacacc gcgtatggct tgcctttttg
    30541 atgctgctgg ttatgttctc tatggcgggc attccgccgc tgatgggctt ttacgccaaa
    30601 ttcggcgtga ttatggcgct cttgaaacaa ggccatgttt ggttgtccgt atttgccgtc
    30661 gtgatgtcgc tgattggtgc gttctactac ctgcgcgtgg tcaaagtcat gtattttgac
    30721 gaatccggcc gcgcccgtcc caccgtcggc ggcaacaatg ccgcaaaatt ccttttgagc
    30781 gtcaacgcgc tgctgttggt gctgtggggt atcatgccgc aaaccgttat cgactggtgt
    30841 gccaaagcat tggaaaacac gctgtaagcc gccgcaacgg cagccgtgtc agaggctgcc
    30901 gtttttgtta agatatgccg ttccgcaatg cagttcagac ggcatcgctg ccgacaacgc
    30961 ccaaacagaa agcccaccat gaccgcatcc atgtacatcc ttttggtctt ggcactcatc
    31021 tttgccaacg tcccctttct cacgaccaga ctgttcggcg tggccgcgct caagcgcaaa
    31081 catttcggac accacttgat cgagctggcg gcaggtttcg cgctgaccgc cgttcttgcc
    31141 tacatcctcg aatcccgtgt gggagcggta cacaatcagg gttgggagtt ctacgccacc
    31201 gtcgtctgcc tgtacctgat ttttgcgttt ccctgtttcg tgtggcggta tttttggcac
    31261 acgcgcaaca gggaatagac aagcatagaa atgccgtctg aaaccttttc agacggcatt
    31321 tgtttcattc aagtgcaggc cggcatcgct gtgccggcac gtttcagccg gcgatatacg
    31381 ccggttttaa tatttgcggg cgactgcaaa ttctgccaac tgccgcaggc gcagggcttt
    31441 gtcgccgaag ggttcgagca gcgcgaccgc ttcggcaacc agtttgtgtg cgtatgagcg
    31501 cgccgcttcc aagcccatca gtctcacata agtcggcttg tcgttgtctg cgtctttgcc
    31561 cgccgttttg cccaaaatcg ccgtgtccgc ttcacaatcc aacacatcgt caatgacttg
    31621 gaacgccagc cccagttttg ccgcataatc gtccaatacc gccagactgt cttcagacag
    31681 attcgggcag gacatcgccc ccaataaaac cgccgcacgg attagcgcac ccgttttcag
    31741 gctgtgcatc tgttccaaat cggcttgaac catttgtttg ccgacatttg ccaaatcgat
    31801 tgcctgaccg cccgccatgc ccgcgctgcc gcccgctttc gccagcaccg acaacattgc
    31861 caactggcgt gcggcgggca gttctgtcgg acggctcaat acgtcgaagg cttgggtttg
    31921 taaagcatcg ccggtcagaa gggcgtttgc ttcgccatat ttgatgtggc aggtcggttt
    31981 gccccggcgc aggctgtcgt tgtccatcgc cggcatatcg tcgtgaacca gcgaataggc
    32041 gtgaatcatc tcgattgccg ccattgcctg ttctactgct tcatgcacgg cttcgcctaa
    32101 ttccgaagcc gccagcacca gcatcggacg cagacgcttg ccgccgtcca aagccgcata
    32161 acgcatcgct tcgtgcagcg tgtgcgggat ttcgttttca gacggcaaaa agcgttccag
    32221 cagcagctct gtctgcgcct gcgccctaag ctgccatgct ttcaaatcat tcgtcggatt
    32281 caaggttcaa ctccttcggt ccgtccgcat ccaaaacctg taatttctgt tcgacttgtg
    32341 ccagcttggt ttgacagtat ctgaccagct cgttgccttc ctgataggcg gcaagcgcgt
    32401 cttccaaggg catttcgccc tgcatagact gcgtcagcga ttcaaggcgc gacagtgcct
    32461 cttcaaacga tttcggggcg tttttcttca tagtttcggg tgttccggta aaaagcggat
    32521 tgtagcattt ttgcaaaacg gatgccgtct gaaacccgaa tccggcttca gacggcattg
    32581 ttttgtcctg caaacggcaa ggcgttaccc gggcagttcg tcggtgatgc cctgcaaaaa
    32641 ggcgaggcgt tcggggcttg ccgccctggt ttcggcggcg gctttgaagg cgcagccggg
    32701 ttcggcgcgg tgggtgcagt tgtggaagcg gcattgtccg acaaggtggc ggaaatcggg
    32761 gaaatagcgc ggcaaatcgg cggcttggag gtggtgtaaa ccaaattctt gcaaacccgg
    32821 ggagtcgatg agttgggttt cgccgttcaa atcataaagc cgggcgtggg tggtggtgtg
    32881 ttttcccgag tcgagtgcgg cggaaatgtc gccggtgcgg gcggtttggc tgcccaaaag
    32941 ggcgttggtc agggtggatt tgcccatacc gctctgcccg agcaggatgt tgctgtgccc
    33001 ttgcagggcg gggcgcaggc tgccggcgtt ttccagtgcg tgggtttcga tgacgggata
    33061 acctagcgtt tcgtagaatt tgagtttttc gcgccaaagg gcggtttcgg gcaggtcggc
    33121 tttgttcagg acgatgacgg cgcggatgcc ggcggcttcg gcggcaagca gggcgcgctg
    33181 tagcagccgc acgctcggac tcgggacggc ggcggttacg atgaggagtt gggtaacgtt
    33241 ggcggcaatg agtttggttt tccacgcgtc ttggcggtag agcaggcttt ggcgcggtaa
    33301 aaaatcttca atcacaactt gttcggcgtt gacggggctg atgcggacgc ggtcaccgca
    33361 ggcgaaatcg acgcgttttt tgcgggtgct ggcttcgtag gttgtgccgt cgggcgtgcg
    33421 gacgatgtag cggcggccgt agctggcggt aatttgggcg gtgtcgttca tggtttcttt
    33481 ggggttgggt gtggaaatgc cgtctgaaaa cgggtgttcg gacggcatcg gttcagtcgt
    33541 gctgccactc gacgtgttcg ttgaggaagc cgccgctctg gtgcgcccag agtttggcgt
    33601 aaagcccgcg tttttcgagg agttcggcgt gtgtgccttc ttcgatgatg cggcctttgt
    33661 cgaggacgac gagcctgtcc attgcggcga tggtggagag gcggtgggcg atggcgatga
    33721 cggttttgcc gtccatcatt ttgtcgaggc tttcctggat ggcggcttcg acttcggaat
    33781 cgagcgcgct ggtggcttcg tccaaaagaa gaatcggcgc gtctttgagc atcacgcggg
    33841 caatggcgat gcgctggcgt tgcccgccgg agagtttcac gccgcgttcg ccgacgtgcg
    33901 cgtcatagcc gcgccgccct ttggcatcgg aaagttcggg gatgaagccg gcggcttcgg
    33961 cgcgttcggc ggcggaaacc atttcggcat cggtcgcgtc ggggcggccg taaataatgt
    34021 tgtcgcgcac ggaacggtgc agcagcgagg tatcttgcgt gaccaaaccg atttgggcgc
    34081 gtaaagattc ttgggtgatg ccgcttatgt cctgcccgtc gatcgaaacc gtgccgcttt
    34141 gcggttcgta gaagcgcaaa agcaggttga cgatggtgga tttgcccgcg ccgctgcgtc
    34201 cgatcaagcc gaccttttcg ccgggtcgga tggtgaggtt gaagccgttg agcagcggtt
    34261 tgcccgcttc gtaggagaaa tcgacgtgtt caaatttgat tgcgccttgc ggcactttca
    34321 acggcagggc gttcggcttg tcgaggatgg tgtgcggttt ggacagggtt gccatgccgt
    34381 cgccgacggt gccgatgttt tcaaacagcc gcgcggattc ccacataatg tattgcgaca
    34441 aaccgttgac gcgcaacgcc atggcggtgg ctgtagcaac cgcgcccacg ccgacctgcc
    34501 cgttgtgcca gagccagatg cccagtgcgg cggtggagag ggtcagggag gtgttgacga
    34561 tgaagctgca cgaatgcagc agcgtcgcca gccgcatttg ggcgcgcacc gtaaccataa
    34621 attcttccat cgactgcttg gcataggcgg cttcacgcgc gccgtgggag aagagtttga
    34681 cggtggcgat attggaatag gcatcggtaa tgcggccggt catcagcgag cgggcatccg
    34741 cctgccatgc ggcggtttgc cccaatttgg gaatcagcag gcgcatcacc gaagcgaaac
    34801 cgacaatcca gccgataaag ggcagcagca gccatgagtc gagcgaggcg agaatcacgc
    34861 cggaggtaat gaaatacacc gacacataaa cgaccatatc ggcaaccgtc atcaccgcgt
    34921 cgcgcaacgc cagcgcggtc tgcatgactt tggcggacac gcgtccggca aattcgtcct
    34981 gataaaaacc gaggctttgg ttcagcatca ggcggtggaa gttccagcgc aggcgcatag
    35041 ggaacacgcc ctgaagggtt tgcaggcgca cgttggacgc ggcaaacgcc cacgcaaccg
    35101 aaaacaccat catcgccgcc attgccgcca gttcccaacc tttttcggca aacagttcgg
    35161 tgggcgcgta tttgccgagc cactccacga ttttgcccat aaattgaaaa accagggctt
    35221 ccataatgcc gatgccggcg gtcagcgcag ccagggcggc tatccatttc cgcacgccgg
    35281 tcatgctgct ccagacaaac cgccacaagc ctttttctgg cgttttcggg gcggcttcgg
    35341 gataagggtc gattcgggac tcgaaccagg aaaatatttt gttcaacatt gttttcgatt
    35401 tcggtaaaac agtttcagac ggcatcaaac acaatgccgt ctgaaaggaa ggacaataac
    35461 gccattttac gggaaaagcc gtcgggaaga cagcgcgggg cggaaacgca gggtttcgtc
    35521 agggcaaacg ccgcgccgcc ttcagacggc attatttcag caggtttttc aaagcaaggc
    35581 gcacgccttc gcccacgtcc gtcccctccg gaacgccttt gaccgccgct tttgcttcgc
    35641 gttcgctgta acccagcgca agcagcgtgc tgacgatgtc ttccgtttcg tcggcggcgg
    35701 gtgcggcggc aaacagcccg tccgttaccg tatgcgcgac cagcttgccg cgcagttcca
    35761 agaccatacg ttcggcggtt tttttgccga ttcccggcgc ggaagacagg cgtttgacat
    35821 cttcttctgc aaccgcctgc gccagctcgt ctgccgtcat cgccgacaaa atgcccaaag
    35881 cggtttttgc cccgatgccg ctgaccttga tcagttggcg gaatgttttg cgctcttccg
    35941 cagtggcaaa accaaataaa agatgtgcgt cttcccgaat gataagctgg gtaaacagtt
    36001 gtacgctttc acccacgggc ggcaggttgt agaaggtctg catcgatacg tcggcctcat
    36061 agccgacacc gttgacatcg atgacgattt gcggagggtt tttttcaacc agtttgccgg
    36121 tcagtctgct gatcatgtgt gccgaatcct gaagtgtcgg gtgcaaaatg ccgtctgaaa
    36181 ccggttcggg cttcagacgg cacggattgt atcaaattca gtcgtcgcgg cgggaggaaa
    36241 tcacgcggcc ggtacgggca tcgacaacga ctttgtattc ctgtccgttt ttgacgattt
    36301 cgacatcata gtgcggacgg ccgttgtcgt gttcgagatc gatgtcggtg attttgccgc
    36361 cgacacgcgc caacgctgct ttttcggctt gggcgcggct gatgattttg tcttgtttgt
    36421 tgtgttggtg tgcggcgtgt ccgtggtcgt catcgccgtg tccgtcgtgg tgggcgagtg
    36481 cgggggcgga aatgcccatc agtgcggttg cggcggcggt caagagaagg cgtttgaagt
    36541 tcatgttttg ctcctgcgaa gtatctggtg gtgtttgaaa gacgtaaagg cgggacatca
    36601 accggcggtc aataccgtct gaatcatacc gcgcgcctga gcttcggcct cggcggcgcg
    36661 ttcctgcgag gcaaacggac ccattttgac gacgtattcg taacggcgtt tttcaatggc
    36721 gaggttcggg cttgatgtcg aagcggcgaa gttttgggcg gcttggttca gataggcttg
    36781 tgcttcgtgt tccgtaccga aagatttcaa gtcgataaag atgtctttgt tttcggcaac
    36841 cggtgtggat tggcccggga cgatttgttc gattttgacg tgtgccgtcc cttggccgac
    36901 aaagcccaat ttttgcgcgg cggctttgga tacgtcgatg atgcggttgc cgtggaaggg
    36961 accgcggtcg ttgacgcgga cgatgacgct tttgccgttt ttggtattgg ttacgcgcac
    37021 atagctgggg atgggcaggg ttttgtgggc ggcggtaaag gcgttcatat cgtatcgttc
    37081 tccgccggaa gttttgcgcc cgtgaaacct gccgccgtac cacgaggcgt tgccggtttg
    37141 cgtgaattcg gcgacttggt ttttcggcgt gtagcgtttt ccggcgactt tgtagctgcg
    37201 gttggcggag gcgtgcagtt tttctgcctt gaccactgcg tcggcggatg ccgtctgaag
    37261 ggagtgtgtg ccgaatgcgg cggtgagaag gaaaagggtt tttcgggtta aagtcaaaac
    37321 gtgttccgtt cttgagttga agacgaatgg gcatcatgcc cgccggatac gttccgaacc
    37381 gccgtacagt gcggacggcg gttcggaatg tgtccggata ggttttcaga cggcatgaac
    37441 ttgcgttcaa acgccgcctg cgtaaccgtg ttgccgccac gcttcaaaga gaatcacggc
    37501 aacggtattg gaaaggttca tactccgact gccgggctgc atgggcaggc ggattttttg
    37561 cgcggcgggc aggctgtcga ggatgtcggc aggcagtccg cgcgtttccg gcccgaacag
    37621 taaaacgtcg cctttttgaa acgcggtttc atcggggcgc gccgtgcctt tggtggtcag
    37681 ggcgaaaatg cgcctgcctg cgagtgcctt gaggcagtcg tcgaagtttt cgtacaccgt
    37741 caggctggcg aattcgtggt agtcgagccc ggcgcgtttc attttggcgg aatccaatgg
    37801 gaagccgagc ggtttgacaa ggtgcaaatc cgcgccggta ttggcgcaca ggcggatgat
    37861 gttgcccgtg ttcggcggga tttccggctg gtataaaacg atggtaaaca taaatatcaa
    37921 tcacttatag gcgcgtaacc ttgccacaag gcggatgggg tgtcaaaaaa tttagttatt
    37981 ttttcattgg cgtgcgtgcc agcgtccagc agcagattcg gtttgcgccc gattttttca
    38041 gcgtctttgc caattcgtcc agcgtcgcgc cggtggtaaa gacatcgtcg attaacagaa
    38101 tattacagtt ttccggtatc ggtgtgcgga tttcaaaggc gtttttgatg tttcgccgcc
    38161 gttcgccgcc tttgagcgtg ctttgcggcg ggcggtggtg tcggaaaacg gtgtgtcggg
    38221 gcagtatctg ccagccgtag cgttgtgcca gcagcccgac gatgctttca ctttggttga
    38281 acccgcgttg cagcagccgc tccctgctta gcggtacggg caggacgaaa tcgaaacatt
    38341 cgtctgtaag ccggtcgggc ggattctgca tcatcaggtc tgccagcggc tgcaccatgc
    38401 tcaaatcagc caagtgcttc agcgcgtgta tcatattgct gacgggcggt tcgtaatgca
    38461 gcgaagccca catccggtcg aatgcgggcg gttttttctg acagccgccg cacaccgcgc
    38521 cgccttggat gtgtctgaaa cacagggggc agctgtttgc cgcgtcggtg cggtatgccg
    38581 ccaaatccgc cgcgcagccg gcgcagatgc cgtctgaaac gccggacgaa ccgtggcata
    38641 atacgcaacg cctgatagtg ggcgcgtctg cgatgcgccg ccaacaagag agaaaatcca
    38701 tgcctgatgc cgtcaaaaaa gtttacctga tacacggttg gggggcgaac cgccacgtgt
    38761 tcgacgattt gatgccgcgc ctgcctgcaa cgtggccggt gtccgccgtc gatttgcccg
    38821 gacacggcga cgcgcccttc gcgcaaccct tcgatattga ggcagccgcc gacgctgttg
    38881 ccgcacaaat cgatacgccc gccgacattc tcggctggtc gctcggcgga ttggtcgcgc
    38941 tgtatctggc ggcgcgccat cccgacaaag tccgttcgct ctgcctgacg gcgagtttcg
    39001 cacggctgac ggctgccgaa gattatcccg aagggcttgc cgcgcctgca ttgggcaaaa
    39061 tggtcggcgc gttccgaacg gattatgcca aacatatcaa acagtttcta caactccagc
    39121 ttttgcacac gcatgatgcg gacagaatca taggcagaat cctgcccgat ttggcgcgct
    39181 gcggcacgcc ctccgcctta caggaagctt tggacgcggc ggaaagggcg gatgcgcggc
    39241 atttgttgga caagatagat gttccggtac tgctggtgtt cggcggcaaa gacgcgatta
    39301 caccgccgcg tatgggtgag tatctgcatc ggcatttgaa aggaagcagg ctggttgtga
    39361 tggaaaaggc ggcacacgcg ccgtttttga gccatgcgga agcgtttgcc gcgctgtacc
    39421 gcgactttgt cgaaggggtt tgacatgaat ccgcaggaca aatgttggca ggttcaccgc
    39481 catcttgccg aacatgccga ccaacggctg acactcgtcc gcaacgcgcc caaacatatc
    39541 ctgcttgccg gggcggatgc ggacatcagc cgcagcctgc tggcgaaacg atatccgcag
    39601 gcggtatttg aagaatacga ttcccgtgcg gattttttgg cggctgccgc agccgtgcgc
    39661 aagggcggtt tttggcaaag gtttacgggt aagggcgtgg tgcaacactg ccaatccccg
    39721 accgcgccgc tgcccgaagc gtgtgccgat atgttgtggt cgaatctcgg actgttggcg
    39781 gcggaacaaa tccttcctgt gctgcacaac tgggcgcgcg cgctggagac ggacgggctg
    39841 ctgtttttca cctgcttcgg gcgagatacc ttggcggaac tgaaatgccg tctgaaagaa
    39901 aatggcattg aaagccgcag cgcgcttttc cccgatatgc acgacttggg cgatatgctt
    39961 gctgaaaacg ggttttacga ccccgttacc gatacggcga agctggtgtt ggattacaaa
    40021 aaggcggaaa cgttttgggc ggatatggac acgctgggcg tttggcgggc gatgtcgtgg
    40081 aacgatgaaa acgccgcgcg ttcgtgtgtc gggacaatat ttgaacggga aggcggtttg
    40141 agtattacgc tggaaacagt gtacggacac gccgttaaaa aactgatgct gccgcaaggg
    40201 gagaacgtgg tgcagttttt tccgaagaga tgatgtgcag atgccgtctg aagctgtttc
    40261 caagtttcag acggcatctg tctgtgaaaa ccgatagaaa taaaggaaat gccgatgtat
    40321 gccgatgccg gagacgtata ttgcgtctat aacttcagac tgaagcagta cactgcctgc
    40381 caggttaccc gagttgaaga acacggtggg aaaaaagcat atgcaaccct gctggctttg
    40441 gactggcagg gcaacaaacc gcttggggcg gcggagctgg cggatttgaa accgctttac
    40501 aaagacttta tgtattggga acgcggtctg cacatgtata aggcaagtgc cgtcgtgccg
    40561 acgggatatg tacgggttgg gaataccgcg ccgctggtcg gcgaagacac gcaacggtat
    40621 gcctcttttt ggggcgacgg ctacgacgtg taccgtcagt tgagatggca gcagataccc
    40681 gaaaaacaga gaaaggcatt caaaaaagcc gccaaaagca aaaagaccgt gatgtttgcc
    40741 ggacgggaat acggcatatc caaacagaat ttgagcgatg tttgggatga ttttgaagac
    40801 gcgatggaac tgaaggcgtt tccctgcctg tcttcgctgt ttctgaccaa atggcataaa
    40861 aatctatatg atagtggatt aacaaaaacc agtacagcgt tg
//
LOCUS       DAWFKN010000009        40060 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFKN010000009 DAWFKN010000000
VERSION     DAWFKN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.009"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(168..2774)
                     /gene="acnD"
                     /locus_tag="WGR46_000454"
     CDS             complement(168..2774)
                     /gene="acnD"
                     /locus_tag="WGR46_000454"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0855461.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVVLTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2855..3643)
                     /locus_tag="WGR46_000455"
     CDS             complement(2855..3643)
                     /locus_tag="WGR46_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ0855462.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFMLHGSIIFPIAATMAAGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(3795..4949)
                     /gene="prpC"
                     /locus_tag="WGR46_000456"
     CDS             complement(3795..4949)
                     /gene="prpC"
                     /locus_tag="WGR46_000456"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0855463.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5035..5910)
                     /gene="prpB"
                     /locus_tag="WGR46_000457"
     CDS             complement(5035..5910)
                     /gene="prpB"
                     /locus_tag="WGR46_000457"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0855464.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6536..7846)
                     /locus_tag="WGR46_000458"
     CDS             complement(6536..7846)
                     /locus_tag="WGR46_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0855465.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(7985..9163)
                     /gene="ftsZ"
                     /locus_tag="WGR46_000459"
     CDS             complement(7985..9163)
                     /gene="ftsZ"
                     /locus_tag="WGR46_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0855466.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9282..10526)
                     /gene="ftsA"
                     /locus_tag="WGR46_000460"
     CDS             complement(9282..10526)
                     /gene="ftsA"
                     /locus_tag="WGR46_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0855467.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10612..11340)
                     /locus_tag="WGR46_000461"
     CDS             complement(10612..11340)
                     /locus_tag="WGR46_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0855468.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            complement(11330..12244)
                     /locus_tag="WGR46_000462"
     CDS             complement(11330..12244)
                     /locus_tag="WGR46_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0855469.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKSRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPSIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12357..13766)
                     /locus_tag="WGR46_000463"
     CDS             complement(12357..13766)
                     /locus_tag="WGR46_000463"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0855470.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFVDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(13921..14988)
                     /gene="murG"
                     /locus_tag="WGR46_000464"
     CDS             complement(13921..14988)
                     /gene="murG"
                     /locus_tag="WGR46_000464"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0855471.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADIAGLPAPDERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRNKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(14992..16317)
                     /locus_tag="WGR46_000465"
     CDS             complement(14992..16317)
                     /locus_tag="WGR46_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0855472.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16443..17780)
                     /locus_tag="WGR46_000466"
     CDS             complement(16443..17780)
                     /locus_tag="WGR46_000466"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0855473.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDTFCCAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPRNELLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRHDLDGCGLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(17864..18136)
                     /locus_tag="WGR46_000467"
     CDS             complement(17864..18136)
                     /locus_tag="WGR46_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0855474.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     NNLGVMYAERRGVRQDRALAQEWLGKACQNGYQDSCDNDQRLKAGY"
     gene            complement(18251..19333)
                     /locus_tag="WGR46_000468"
     CDS             complement(18251..19333)
                     /locus_tag="WGR46_000468"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0855475.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19427..19603)
                     /locus_tag="WGR46_000469"
     CDS             complement(19427..19603)
                     /locus_tag="WGR46_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855476.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(19606..20964)
                     /locus_tag="WGR46_000470"
     CDS             complement(19606..20964)
                     /locus_tag="WGR46_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0855477.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21089..22734)
                     /locus_tag="WGR46_000471"
                     /pseudo
     CDS             complement(21089..22734)
                     /locus_tag="WGR46_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(22757..24235)
                     /locus_tag="WGR46_000472"
     CDS             complement(22757..24235)
                     /locus_tag="WGR46_000472"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0855478.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24260..26005)
                     /locus_tag="WGR46_000473"
     CDS             complement(24260..26005)
                     /locus_tag="WGR46_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0855479.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(26066..26329)
                     /gene="ftsL"
                     /locus_tag="WGR46_000474"
     CDS             complement(26066..26329)
                     /gene="ftsL"
                     /locus_tag="WGR46_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0855480.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(26326..27312)
                     /gene="rsmH"
                     /locus_tag="WGR46_000475"
     CDS             complement(26326..27312)
                     /gene="rsmH"
                     /locus_tag="WGR46_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0855481.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(27309..27764)
                     /gene="mraZ"
                     /locus_tag="WGR46_000476"
     CDS             complement(27309..27764)
                     /gene="mraZ"
                     /locus_tag="WGR46_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0855482.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            28043..29260
                     /locus_tag="WGR46_000477"
     CDS             28043..29260
                     /locus_tag="WGR46_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0855483.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            29392..29468
                     /locus_tag="WGR46_000478"
     tRNA            29392..29468
                     /locus_tag="WGR46_000478"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29426..29428,aa:Met,seq:cat)
     gene            29476..29551
                     /locus_tag="WGR46_000479"
     tRNA            29476..29551
                     /locus_tag="WGR46_000479"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29509..29511,aa:Ala,seq:ggc)
     gene            complement(29842..30663)
                     /locus_tag="WGR46_000480"
     CDS             complement(29842..30663)
                     /locus_tag="WGR46_000480"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0855484.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLVGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGIAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(30697..31341)
                     /locus_tag="WGR46_000481"
     CDS             complement(30697..31341)
                     /locus_tag="WGR46_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0855485.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQLEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVRAERKLSNHPVQK"
     gene            complement(31344..32189)
                     /gene="ftsN"
                     /locus_tag="WGR46_000482"
     CDS             complement(31344..32189)
                     /gene="ftsN"
                     /locus_tag="WGR46_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0855486.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(32295..33791)
                     /locus_tag="WGR46_000483"
     CDS             complement(32295..33791)
                     /locus_tag="WGR46_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0855487.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPIKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(33805..34125)
                     /locus_tag="WGR46_000484"
     CDS             complement(33805..34125)
                     /locus_tag="WGR46_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0855488.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34608..36134
                     /gene="putP"
                     /locus_tag="WGR46_000485"
     CDS             34608..36134
                     /gene="putP"
                     /locus_tag="WGR46_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0855489.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     NVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(36224..36391)
                     /locus_tag="WGR46_000486"
     CDS             complement(36224..36391)
                     /locus_tag="WGR46_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855490.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            36383..39988
                     /gene="putA"
                     /locus_tag="WGR46_000487"
     CDS             36383..39988
                     /gene="putA"
                     /locus_tag="WGR46_000487"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0855491.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDKNISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNDKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         9446 a        11348 c         9968 g         9298 t
ORIGIN      
        1 tccgcacaga aactcatcgg ataaataagg tttcctcaat tccactttct ggattcccgc
       61 ctgcgcggga atgacgattt acagtagatg cctgccatat cgagaattac gtgaatgagc
      121 aaaatgttga acccgaccca cgctacgtct tgcttttcag acgacctcta agccgcgttc
      181 ccttccaaaa aatcctgtgc aaaccgttgc aacacgccgc cggcttcata taccaatact
      241 tcttctgcag tatcgaggcg gcaggtaacg ggaacttcaa cggtttcgcc gtttttacgg
      301 tgaatcacga gggtcaggtc gcagcgcggt gtgcgttcgc cgaccacgtc gtaggtttcc
      361 gtaccgtcca gttgcagggt atggcggttg gtgtcgggtt tgaactgcag cggcaacacg
      421 cccatgccga taaggttggt gcggtggata cgctcgaagc cttcggcaac aatcgcttct
      481 acgccggcga ggcgtacgcc ttttgcagcc cagtcgcggc ttgagccttg accatagtcc
      541 gcaccggcaa tgatgatgag cggctgtttg cggttcatat aggtttcgat ggcttcccac
      601 atgcgcatgg tttcgccttc gggttcgacg cgggcgaacg agccttggcg cacgctgccg
      661 tcttcgtttt tcaccatttc gttaaacagt ttcggattgg cgaaggtagc gcgttgggcg
      721 gtcaagtggt cgccgcggtg ggttgcgtaa gagttgaagt cttcttcagg caaacccatt
      781 ttcgccaaat actcgcctgc ggcactgacg gccaaaatcg cattggacgg cgagaggtgg
      841 tcggtggtga tgttgtcggg caaaatcgcc agcggacgca tacctcttaa tgtgcgttcc
      901 cctgccagcg cgccttccca gtaaggcgga cggcggatgt aggtggacat cggacgccaa
      961 tcgtacagcg gactaggtgc tttttgcgct gtgccggtgt cgaacatcgg tacatacaca
     1021 tcgcggaact gctgcggttt cacatattcg gcaacgacgg catcgatttc ttcatcggca
     1081 ggccaaatgt ctttcaggcg gatttccttg ccgtctgcaa cgccgagtac gtcgttttca
     1141 atatcgaaac ggatactgcc tgccagcgcg taggcaacga ccaacggagg cgaagcgagg
     1201 aaagcctgtt tcgcatacgg atggacacgg ccgtcgaagt tgcggttgcc tgataatacg
     1261 gcggtggcgt acaaatcgcg gtcgatgatt tctttctgga ttttcggatc cagcgcgcca
     1321 ctcatgccgt tgcaggtggt gcaggcgaag gcgacgatac cgaagccgag tttttccatt
     1381 tcgggcagca ggcctgcttc tttcaaataa atttccgcca ctttcgaacc gggggcaaac
     1441 gaggttttga cccacggttt gcgtttcagc ccgaagcggt tggcgttgcg cgccaagagc
     1501 gcggcggcaa cgacgttgcg cgggttggaa gtgttggtgc aactggtaat cgcggcgatg
     1561 atgaccgcgc cgtcgggcat ttgtccgtct gaaggctctt cgtaaggctt cgccagccct
     1621 ttcgccgcca aatcgacggt ggcaaaacgg gcatgcgggt tacttggacc tgccatattg
     1681 cgcgttacgc tgctcaaatc aaatttcaaa acgcgaggat aaacggcggt tttcaaggca
     1741 tctgcccaca agcctgcggt tttggcgtag gtttccacca atttcacctg cgcgtcgtcg
     1801 cgtccggtca gtttcaaata atcaatggtt tgctcatcaa tagcgaacat cgcggcagtc
     1861 gcgccgaact ccggcgtcat gttggaaatg gtcgcgcggt cgccgataga caggcttctc
     1921 gcgccctcgc cgaagaattc gacaaacgcc ccgaccacgc gttctttgcg cagaaactcg
     1981 gtcagtgcca acacaatatc cgtcgccgta atgcccgcct gccgtttgcc ggtcagcacc
     2041 acaccgacaa tatcgggcag gcgcatcatg gacgcgcgtc ccagcattac ggtttccgct
     2101 tccaatccgc ccacgcccac ggaaatcacg cccagcgcat cgacgtgcgg cgtgtgcgaa
     2161 tccgtgccga cgcaggtatc ggggaacgcc acgccgtttt tgacttggac gacgggcgac
     2221 attttttcca gattgatttg gtgcatgatg ccgttgcccg ccggaatcac gtccacattt
     2281 tcaaaggcgg ttttcgtcca gttgatgaag tggaaacggt cttcgttacg tctgtcttcg
     2341 atttggcggt ttttgcggaa ggcatcgggg tcgtagccgc cgcattcaac ggccaaagag
     2401 tggtcgacga tgagctgggt ttgcaccacc ggattcactt tggcaggatc gccgcctttt
     2461 tcggcaatcg catcgcgcag acctgccaaa tccaccaacg cggtctgccc cagaatatcg
     2521 tggcacacca cccgcgccgg ataccaagga aagtcgattt cctgttttcc ctcaatcagc
     2581 tgacccagcc agctttgcag cgtcggcaaa tcgactttgt ccgcgcggtt gaccaaattc
     2641 tccgccaaaa tgcggctcgt gtaaggcagc ttgtcgtaag agccgggctt gatgtcctca
     2701 cacgccgcac gcgcgtcgta gtattccaaa tccgtaccgg gcagcggttt gcggtaacgt
     2761 tggttggcag ccatgttcag ttctcctgtg gatctatttt tcttgtggtt tggggtttca
     2821 gccgacgttt gtcgaagggg tcgtctgaaa gggtttaaaa catcgaaaca atcatctgat
     2881 acagcggatt tctctcgtct atcaacaatt tcacagccat cgaaatgctg atgacaatca
     2941 gcagcggctt aatcagcttc gaaccgaagc ggacggcaaa tctcgcacct aaattcgcac
     3001 cgacaaacgc accggccgcc atcgttgccg caatcggaaa aataattgaa ccgtgcagca
     3061 tgaataccga tagcgaacca agattgcagg caacgttcgc caatttggtg taagacatcg
     3121 cgttcaacag cttgcagccg agcaaaacaa taaaggcaat cagaaaaaac gagccgacac
     3181 ccggtccgaa cacaccgtcg taaaaaccca aaagcggtgc gaccgtcagc ccgaacagaa
     3241 aaaaagacat tctggctttg ccttccttac tgccgtcgag cttgggcgaa aacacaaaat
     3301 acagtgcgac aaatatcaac aaaaccggca cgaccgccag cagaatatct ttggaaacca
     3361 agctgaccga taatgcaccg gccacgccgc ctacaaacga tgctgcggca atcgggagac
     3421 ctttcttcca atcaatcaaa cctttgcgtg caaaagaaac cgtagctgaa aacgtagcag
     3481 cggctgcttg cagcttgttg gtggcaattg ccgacacggg aggaatacct gccaacaaga
     3541 gtgcgggcag cgtaatcaaa ccacccccgc ccgcaatcgc atcgataaat ccggcaatca
     3601 ttgcaaccaa acccaaagcg agtattatgt ataaatcttc catgtttctt atcctgttac
     3661 ttgcgccaat acaggattat cttctcctat tagattaaac ttatttcaga caacctttcc
     3721 aataaggcaa ggtcgtctga aatccttagc tttgcatacc gaaataaaga acaaactgct
     3781 attgcattct tcaattatcg ttcttcaatc tccacaaacg ccaaatcttc agggcctgtg
     3841 tagttcgcgc tcggacggat gattttgccg tctttgcgtt gctcaagaac gtgtgcgctc
     3901 caaccggttg tacgggaaat tacgaacagc ggtgtgaaca tagcggtcgg tacgcccaat
     3961 ttttggtagg aaacggcaga gaaccagtcc agattcggga acattttttt ctcttcccac
     4021 atcacgcttt ccaaacgttc ggcaatgtca aagaggcgca tatcgccggt ttctttgctc
     4081 aaaccgcgtg ccacttcttt aatgacaacg ttgcgagggt cggaaatggt gtacactgga
     4141 tgaccgaaac cgatcacgat ttctttgcgg ccgatgcgtt cgcggatgtc tgcttcggct
     4201 tcgtcggcat tgcggtagcg tttttgaata tcgtaagcca cttcgttcgc gccgccgtgt
     4261 ttcggacctt tcaacgcgcc gattgctccg gtaatgctgg agtacatatc agagcctgta
     4321 ccggcaatca cgcgggcggt gaaggtagaa gcgttgaact cgtgttcggc atacagaatc
     4381 agtgaaacgt gcatggcttt gatgtgggat tcgcttgggc gtttgccgtg caacagttgc
     4441 aggaaatgac cgccgatggt ctcttcgtcg ctttcaacct caatgcgttt gccgttgtgc
     4501 gaatattgat accagtacaa gaggatgctg ccgaggctgg cgatcagttt gtcggcgatg
     4561 tcgcgcgctt cactttccgg atggctttca cgttcaggat gaacgcagcc cagcatggat
     4621 acgccggtac gcattacgtc catcggatgg gtatgtgcag gcaggctttc caaaacttta
     4681 atcacacgga taggcaggcc gcgcatggat ttgagcttgg ttttataagc ggccagctcg
     4741 aatttgttgg gcagatgacc gtgaatcagc aggtgggcga cttcttcaaa ctcgcatttt
     4801 tgtgccaaat ccaagatgtc gtaaccgcga tagctcaaat cgttgccggt acggccaacg
     4861 gtacacaaag cggtattacc ggccgcaacg cctgaaagcg caacggattt cttaggtttg
     4921 aaggtcgggg tttgagtagt ttcagtcatg gtatttctcc tttgtgtttt tatgggtttc
     4981 gggttttcag acgaccgatg cggatttgtt gaaagaccgt ctgaaagcgg taaatcattt
     5041 ttgaaacaat ttatccagtt tttgctcgaa ggcatgatag ttcagatgct cgtacagctc
     5101 ggcacgggtt tgcatactgt ccaccaccgc cgcctgagtg ccatcgcgca taatcgcttc
     5161 gtaaacattc agagcggctt tgcttgctgc acggaacgat gacagcggat acagcaccag
     5221 cgacacgccg ttttcagcca gctcgctttg ggtataaagc ggagtggaac caaactcggt
     5281 aatgttcgcc aacacgggca ctttcaccgc atctgcaaat tggcggtaca tgttcaaatc
     5341 ggtcatggct tcagggaaaa tcatgtccgc accggcttcg acacaagctt gggcgcgttc
     5401 gatagcggca tccaaacctt ctaccgccag cgcatcggta cgcgccataa tcacgaagtt
     5461 ctcatcaacg cgcgcatcta cggcagcttt gatacggtcg accatttcat ctttagatac
     5521 aatggctttg ttcggacggt ggccgcagcg tttttgcgct acctgatctt cgatgtgaac
     5581 cgctgcaaca ccggcgcgtt caaagttgcg aatggtacgg gcaatattga atgcaccgcc
     5641 ccaacccaca tcgatgtcca ccagcagagg cgtatccacg ttgtccgtaa tgcgtcgtgc
     5701 gtcgatcagc acatcttcca ttgtggtaat gcccaaatca gggataccgc aagaacaggc
     5761 tgccacgccg ccgccggaca gatagatggc tttgaaaccg ctttgggtgg ccaatcgtgc
     5821 aaaataagca ttgacgcaac cggcgacggc aagcggattc gattctttca cggcttggcg
     5881 gaaacgtgct ccggcagagt gttgactcat catatttctc ctttatagac tttttttcag
     5941 tatggacagg cttccatcac attcggacgg caaaacacag ccatccgacg cgtcgggcat
     6001 cccaatccaa tcattaaaaa tatatgggaa aaattatctt attgatattt aaaacgaatc
     6061 aaagaaaaac agcagaccgt tcggaattat gcggcaaaac cgcagacaag aagaaaacaa
     6121 ggggattatt cagaaaaggg gaaaacatct gaattggttt catagtaatg ttcctttgta
     6181 gtgttatgta gttttatttt tcgataatct aaaacacaag actgaaaatg tcaaacattt
     6241 ttaaggagac tcattacata attttaatat tttattttaa ttccatgatt aaactagatt
     6301 aattttgaat tatttgtttc aataaaattg tcaggaaatc aagaaaaatt ttcgataatc
     6361 aaaatcctga aaacttataa aatattttat acataaaaac aatgtattat ttttaagcaa
     6421 tccgatgtat tttattatgt atttgatttt tatataattt aataaaaatt aaatatatcg
     6481 gcaaacaaaa tgccgtctga aaacctttca gacggcattt ttaatatcga atcaatcagc
     6541 ccaaatacca gaatttcagt gcccacaata cggcaacaat ccataccata ggcggaacgt
     6601 ctttggtgcg gcggcataaa agtttaacca cggcatagct gatgaagccg aaggcgatgc
     6661 cgtctgcaat cgaataggta aacggcatga agacaatggt caggaatgcg ggtgcggctt
     6721 ccgtcatatc gtcccaatca atatccctcg cactgcggag catctgcgtg ccgacataaa
     6781 gcagggcggg cgcggtggca aaagcgggaa cacttttcgc caaaggtgaa aacatcaggc
     6841 aggcgagcat caatacgccg actgtaaccg ccgtcaggcc ggtccgtccg cctgccgata
     6901 cgcccgccgc gctttccaca taaggcgtgg tggaagaagt acccaaagcc gcacctgcca
     6961 caatggcggt agagtctgca agcagtgcgc gtttcaggcg gggcagctta ccgtccacca
     7021 gcagcccggc acggtgggat atgccgacca gcgttccggt actgtcaaat agatcgacca
     7081 agaagaagac gaaaatcaca ctgaccatgc tgacggtaaa caggccttca aaatccatct
     7141 gcataaaagt cggcgcaatg ctcggtactt cgccgatgat gccgtgaaat tcattcaaac
     7201 ccatcaggct ggcaatgacg gtaatggtca agatggtgat gatgattgcg ccttgaacgc
     7261 ggaaatgtcc caatacgacc accatagcaa aaccgaacag tgccaacaac gcggacggct
     7321 gatgaatatc gcccaaaccg accaaggttg ccggattggc aacgataatg cctgcgcctt
     7381 tcagggaaat cagtgccaaa aacaaaccga taccggcagc aatcgacatt ttcaaaccca
     7441 taggcagtgc gttgaccagc atttccctga ctttaaaaaa gctgaacagg ataaaaatca
     7501 gaccggagat gaacaccgca cccaacgcaa cctgccaagg cacgcccata cccttaacga
     7561 cggcaaaggt gaaataggca ttcagcccca tccccggtgc gagtgcaatc ggatagttgc
     7621 cgacaaaacc cataacaaaa cagccgatgg cagacgcgat acaggtagcg acgaataccg
     7681 cccccatatc catgccggtc tcgcccaaaa tcagagggtt gacgataacg atgtagcaca
     7741 tcgtcaaaaa agttgtcaaa cccgccatca actcggtacg caccgtcgta ccgtttgcct
     7801 tcagcttaaa aatcccgtcc aacagtgttt gttttgaagt gtccatatct gaaagtctgt
     7861 atcaagtgga aaaatctgaa tccgccgacc gcaaaccgta cgaaattgca acatcttgaa
     7921 aaacatacct cccaaaccgg acaaaccgat tcgggaggca gcaggtttac gggcaaacag
     7981 cacattattt gtctgaattg tgttgacgac gcaaaatcgc agggatttca aagtcgtcaa
     8041 gtacggactg attgtcgaaa tccgcagcgg taaggttcat cgtgcggata ccgcgattgg
     8101 tgcggatcat accttcgaca ttgtggcttt gctcctgttt ggacggagca accgcttcta
     8161 cctcccttgc cggaacaaaa tcgaccgcgc ctttttcttt cagaccggta gcgataatgg
     8221 taatccggat ggcatcttcg ctcatggtct cgtcttcagc cgcaccgaat ttgcattcca
     8281 aatcgggatg cgcgctttgg ttgacgattt tcatgacttc ggacaactcg gacattttca
     8341 agcaacccgg agcagtcgta atattgacca gcacaccgcg cgctccgtcc aaggttacat
     8401 cgtccagcag cggactggaa atggcctggt cggtcgccat acgcgcacgg tcgatacctt
     8461 gggcataacc cgaacccatc atagcgatac cgcggttgct catcacggtt ttcacgtcgg
     8521 caaagtcgag gttgatgatt tcgctcgggc aagttaccac ttcggaaatg cctgcgaccg
     8581 catcgcgcaa tacattgtcg gcggcacgga aggcttcgcg catcgttacg tcttcaccca
     8641 atgcagtcat cagtttgtcg ttcgggatga tgatcagcga atcgacgtgt tctttcaact
     8701 gttctaaccc tgcctgtgcg acatggacgc gcttaccttc atatgcgaac ggtcgggtaa
     8761 ccacggcaac ggtcagaatg cccaaagact tggcaatctc agcaacaacc ggcgcggaac
     8821 cggtaccggt accgccgccc ataccggtcg tgataaacag catattcgca ccgcgaatgg
     8881 cttcttcaat ggcttcccgg tcttcctggg ctgccgcacg gccgatatcg ggattcgcgc
     8941 ccgcgcccaa accgcgtgtc agattcgtac ccaactggat tctcttcgcc gcatggtttt
     9001 ttgccagaga ctgcgcatcc gtattggcac tgataaactc cacaccgcgc acattgttgg
     9061 caaccatgtt attgattgca ttgcaaccgc cgccgcccaa gccgattact ttaatcaccg
     9121 cagggctgac tgccgattct gccacgtcgt aaacaaattc cattcaaaaa ctcctgctcg
     9181 ccccattcag aggacggttt aaataaatta ttattcatta tataagaagt atcttgctgc
     9241 tggcaaaata cttctcacct gtcaaacggc aatccacctg ttcagaagct gttttcaatc
     9301 caccgtttca atcttgccaa caaaccgccg cccccttccc tctcttgcac tgcaccgttt
     9361 tccggctgcg gcaagtttcc ttccagcttg catgctgcat gaagcagccc gatggcggta
     9421 gaaaagcgcg gtgtgcggac gcggtcggac aaaccgccca tttcttgggg cgcaccggtg
     9481 cgtacaggca aatcgaagat tttttcggca aattccacaa tcccggtcat catggacaca
     9541 ccgccggtca gaacaatacc cgcattcagc acttctttgg ggaaacccga tttttgcagc
     9601 tcgcccagca ctacgccaaa aatctcctga atacgtgcac tgatgattgc tgccaaaacc
     9661 ttactggaaa cctgacgcga tgtccggtca cccacgcccg gaacttcaat catctcaccc
     9721 aagccttccg tatcgcatga tgtcacgcca taatggattt taatgtactc ggcggcatcg
     9781 agaggtgttc tcaacgattt ggacaaatct ttggtaatca gattaccacc ggccggaatg
     9841 acggacgtat ggcggatggc gccgttcata taaacggcaa tatcggtcgt tccgccacca
     9901 atgtcgatga cgcatacgcc gaggtctttt tcatcttcag tcagcaccgc ctgcccgctt
     9961 gccaacggct gaagcatgat ctgatcgctt ttcaagccgc accgctcgat acatttttgg
    10021 acattctgca ctgccgtact tgcaccggta atgatgtgca cccgcgtatc caaacgcaca
    10081 ccgctcatac cgataggctc cctcacgcca agttgggtgt cgataatgta gtcttgaacc
    10141 acagcatcga gaattttttg atcgggcggg atattgattg cctttgccgt ttcaatggcg
    10201 cggtcgatgt ctgcctgcgt gacttccccg tctttaattt taaccacacc ttgcgaattg
    10261 agactgcgga tgtggttgcc tgcgatacct gtggtaacgt gggtaatttt ggtatccgcc
    10321 atcagctcgg catcattgac cgcctgcctg atggcttgga cggtggcatc gatattggtt
    10381 accatgcccg cgcgcaagcc ccgtgaagga gcctgcccca aaccgacgat gttgattttg
    10441 tcgtcatctt gaacttcccc gatcagtgcg aggactttag acgtaccgat atccagtacg
    10501 ctgatgtatc tttgctgctg ttccattgtt cgtctgctct taaaactgat tgaaatttgc
    10561 gtcgcaccgt ttcagacggc acggccgtaa tctgtccgat acctgttccc actattcttc
    10621 ggattctttt tcgggtaaac cgtcgtaagc atagcggact gaaaatccgt ccttatacct
    10681 catatccaca taggataacc gatttttatt tttacgcaac agatgctgcc acgcttcggt
    10741 aaaaagccgg aggcgtttca tctcgttttc ccgtccgagc ctgacggtga tgccgttgtc
    10801 caaaacgaca atccacgccg aacgtgccgt ataggtcatc tctttgatgc ccaaaccctg
    10861 ttttgccaaa acagtcgaaa attcgtcata acggcggagc atttcggcag acgttccttc
    10921 cgcgcctctg aataccggca ttccgggtct gtccaagcgg gcttcaaaaa cattgccttc
    10981 gccgtccacc aaggcatggt cgccccaacg cgcgaccggc ttgcgctcgg tcaggacgac
    11041 ctcaaccgtg tcgggaaaac ggcggcgaac catgaccgac gcaatccacg gataccggcg
    11101 gtaggcctcc tgtgcgccat tgatgtccgt cctcaaaata ttcccatgga tgtattcttt
    11161 cgccaaactg cccaatgctt tcttatcgga ataaaccagg ttgcccttca gcgacacctg
    11221 cttgacgggc agatgattcg aattgtaaaa ccaaaccagc ccggacgcag caagcagcat
    11281 cgccatcatg acaagcagcc agcgcgtcag ccgttccatc gcttcggcat tatcccacat
    11341 gtgcggtctt caaaatttca atacataaat cggcaaaacc cacgcccgta acggcagcgg
    11401 atttcggtac taaactatgg ccggtcatac cgggcagggt gttgatttcc aacagataga
    11461 gtttgccgtc ggtatctttg aggaaatcga cgcgcacgca gccttccgca ccgattgcct
    11521 gcgcgccgcg aaccgccagt tcgcgcatca ggctttcttc ggcttcggtc agatcttccg
    11581 aaggacattg ataaatggtg tcgttgcggt tgtacttggc ttcgtagtcg taaaactcgg
    11641 tcgcggggat gatgtgtatg ctgggcaggc ctttgccgtt caacacaggg caggaatatt
    11701 cgccgccgcc gataaaccgt tcggcaatga tttcgccctg aaggtgtttc aattcttcgt
    11761 aaacgctttt cagacggctt tttcctttga cttttaccac gcctacgctg ctgccttcgg
    11821 ccgccggttt cacaaacatc ggcaggccca atttttcttc gacggcatcg aaatcagtgt
    11881 cgtcgtgcag gacggcgaac tcgggaacgg gcaatcccaa tgcctgccaa atcagtttgc
    11941 agcggtattt gtccatgccg atggcggatg cggcgacacc gctgccggta tagggaatgc
    12001 ccaacagttc caatgcaccc tgaaccgccc cgtcttcgcc gtaagtaccg tgaaggatgt
    12061 tgaatgccgt ctgaaaacct tgtgccttca attcagacaa tggggtttct ttaggatcga
    12121 aggcgtatgc gtctatgcct ttgcttttta aagcattcaa aatggcggtg ccgctgtcca
    12181 gcgagatttc tcgttcgctg gaaaaaccgc ccatcaatac ggccactttg ccaaaattct
    12241 gcattgtttt tgttctttcc tgattgcttt atgcttgtta ccagaggtcg tctgaaacct
    12301 gatttgcggt ttcagacgac cttgatatga tgttccgtct gtcaggcggg tgtgcctcaa
    12361 atctgtttcg acaattccag cagcgcggcg gggacgcggt tgatgcttcc cgcgcccata
    12421 ttcaacacga tgtcgccgtc ctgcaaaacg ttcaacagca tttcgggcag atcggcaacg
    12481 ttttcgcagt aaatcggctc gagtttgccc aacacgcgga tggcgcgggc aagagcgcgg
    12541 gaatcggcgg cggcaatcgg ctcttcaccg gcggcataca cttcggtcag caccagcgcg
    12601 tcaacggtat tgaggacttt ggtaaagtct tcaaacaaat cgcgcgtgcg ggtatagcgg
    12661 tgcggctgga aggcgagtac caaacgtttt tccggatacg cgccgcgtgc ggcggaaagg
    12721 gtcgccgcca tttcgacggg gtggtgtccg tagtcgtcca ccaagagcgc ggtcccgccg
    12781 tttggcaact tgatgtcgcc gtatttttgg aagcggcggc cgacgccttc aaagccgagc
    12841 aagccttttt ggatggcttc aaccgatgcg ccgacttcca acgcaacacc gatggctgcc
    12901 aaggcattga gaacattgtg gcggccgggc atattcagca cgacttcaaa cgacccctgc
    12961 tcatgtcctt tcatttgaac atggacggtg aatttcattt gcgcgccgac gttttcgatg
    13021 tcggtggcgt agatgtcggc ggtatcgtcc aagccgtaag tggcataagg tttgctcact
    13081 ttgggcagaa tcgcgcggac gtgttcgctg tcgatacaca aaaacgcttt gccgtagaac
    13141 ggcatacggt ggataaaatc aacaaacgcc tgatgcagtt tttcaacgct gtgtccgtag
    13201 gtatccatgt ggtcttcgtc gatattggtg acgacagaca taatcggggt tagatgtagg
    13261 aaagaggcat cggattcgtc ggcttcggcg acaatgtatt cgcctttgcc caaacgggcg
    13321 ttggtacctg cggcgttgag tttgccgccg ataacgaaag tcgggtcaag tcctgccgcg
    13381 ccgaggatgg aggcggtcag gctggtggtc gtggttttgc cgtgcgtgcc ggcaatggcg
    13441 atgccgtcac ggaagcgcat caactccgcc aacatcaggg cgcgcggaat aacgggaatt
    13501 tgctgctcca acgcagcgac aacttcggga ttttcttttt tgacggcggt agaggtaacg
    13561 acgacatccg caccgttaac gtgttctgcg gtatggccgg gataaacttg aatgcccagg
    13621 ctgcccaaat gctcggtagc ggcatttcgc gcctgatccg aaccggaaac tttaaaaccc
    13681 aaattgtgca agacttcggc gatgccgctc atgccgacgc cgccgatacc gacaaaatgg
    13741 atgttggtca ctcgattttt catcataatg ttgcgttccg gtggattttc gatgcgtaaa
    13801 ggcgttattt taaagggctg accgtttgcg cgccatagtt ttctgacaaa tatatagcgg
    13861 attgaaataa aaaacatcca tgccgtctga acggcttttc agacggcatg gttcggcagt
    13921 ttacgccgca cacgcaatcg cggcttcggc aacgtcgtcc gcgctgtgcg gcaatgccaa
    13981 agtacgggcg ttttctgccc atttgagaca tttttcgcgg ttcaggctgc cgaggatttc
    14041 ggcgagtttt tccgccgtca actgggtttg cggcaacagc aatcccgcct ccgcctgcac
    14101 cataaaacgc gcgttggcgg tttgatggtc atcaacggcg tgaggatacg gcactaacaa
    14161 cgcacccaat cccgccgccg tcaactcggc aatcgtcagc gcgccggcac ggcaaatcac
    14221 caaatcggca tcgcggtagg cggacaccat atcggtaata aattccacgc attcggcttt
    14281 cacgcccagc gcgtcgtaat ccgcctgcaa gctgcccagc ttgttgcgcc ccgattggtg
    14341 gtacatctgc ggacgcgcat tgtcgggcag caaagccaat gcctgcggta cggttttgtt
    14401 caaaacgtct gcacccaaac tgccgccgac caccaaaatt ttcagacggc cttcgcgccc
    14461 ttggaagcgt tcgtcaggcg cgggcagccc ggcaatatcg gcgcggacgg ggttgccgac
    14521 caagccgcct tcgtggctga acgctttcgg aaaagcgtac aacacccgct tcgcccagcg
    14581 cgacaggtgg cggttggaca aacctgccac ggcgttttgc tcgtgaatca caatcggcac
    14641 gcccaagagt ttcgccgcca aaccgccggg gaaggtaacg aagccgccga agccgatgac
    14701 gcactcgaca cggtgtttgc ggataatccg ctgcgcttcg cggacggttt gatacaaagt
    14761 aaacggcagc atcagcttgc gtttgatgcc gttgccgcgc acgcctttaa tcgccagcgt
    14821 ttccaagcgt atgccgtatt gcggcacgat acgctcttcc atcgaatcct tgctgcccag
    14881 ccaaatcaca tgatggccgc gcgcgcgcaa tgaatccgcc accgccagcg cggggaaaat
    14941 atgtccgccc gttccgcccg ccatcagcat aaaggtttta ccgcccatga tttactccac
    15001 ccgataaccg cgcattttcc ggcggttttc ataatctata cgcaacagca gcatcatgct
    15061 gatcagcatg aaaaagactg acgaaccgcc ataggacatc aacggcagcg tcagaccttt
    15121 ggtcggcaaa gcaccgatgt tcacaccgat attgaagaaa ctttggatac cgatccaaat
    15181 gccgataccc gaagcgatat aggcgttgaa agtcaaaccc aaatcgcgcg actgcttgcc
    15241 gatggaaaac gcccgcacca ccagccagcc gtaacagaat atcagcacgc acataccgaa
    15301 gaaaccgaat tcttcggcga tgatggcaaa aataaaatcg gtatgcgctt ccggcagaaa
    15361 gccgcgtttg ctcaaactcg cacccaaacc cataccgaac cactctccgc gcccgattgc
    15421 catcagagag tgggtaagct ggtagccggc accctgcggg tctttccacg ggtccaaaaa
    15481 tgccactacc cgctgcacac ggtagggagc ggcggtaatc atcagcacca tcccgcccaa
    15541 gacgctgcct accaggacga aaaaatattt ccacggcaat cctgccaaaa acagcattcc
    15601 aacggcaatg acggtaatga cgacaaacga accgaaatcc ggctgtacca ttatcagcac
    15661 caaaccgaac gccaccagca taatcggcag gatgatcgcc cggaaacggc cgtacatttc
    15721 taatgtttca cgacgtgcct gcggattggt ggcggacatg atcagattgg ccgtcccccg
    15781 ccaaatcgac tgccaaccca aactttccat gctgcgcaac acttcttcac ggcgcgtgaa
    15841 caggcttgcc aaataaagga tgaccgccag cttgaacagc tcggtcggct ggaaattcaa
    15901 cggacccaaa ggtatccaac gggtcgcgcc attgatttcg cgcccggcaa tcaatacgac
    15961 taccagcaac aggccggata gggcaaaaat ccacggcaca agccgccgcc atgtcctcat
    16021 cctgcaaaga aaccataaaa aaccgctcgc tatcaagccg gcaacgacga accccgcctg
    16081 tctggtcaaa tagaaaaact gatcgccgcc ttcttttgat gccaaataca cagaagccga
    16141 ataaatcatc agcaggctga acgccgtcat cagcaccacc atccacaaaa gcggcgcgtc
    16201 gaatttcctg ccgtcgcgca caatcggcct gtcgagcagc agagtgtgga caccgtcgcc
    16261 cacttttacc aatacttccg aaatcttcaa aaaaaccacc tgccagtctg tttgcacctg
    16321 ccgcaaaggg caaaaatttc agacggcaga caatgccgtc cgaacatacg atacatccca
    16381 aatcggtatt ctaaatcttt acttgccgcc caacaatgac ggcgtttgcg tttcagacgg
    16441 tatcacaaag ccttaaacgc cccgataaac acttccgaac ggtgcgcata gcctttaaac
    16501 atatcgaagc tcgcgcaggc ggggctgagc agcacgatat cgcccgcttc ggcttgggca
    16561 tatgccttct gaaccgcttc ttccaaagtg gcgcagtcgg tcatattcaa accgcagccg
    16621 tccaaatcgt ggcggatttg cggcgcatcg acgccaatca ggaacacgcc ttttgccttg
    16681 ccggcaagcg cgtcgcgcag gggcgtgaag tcctgccctt tgcccatgcc gcccaaaatg
    16741 acgaatagcg gattttgcaa accggcaatc gcagcggcgg ttgcaccaac gttcgtgcct
    16801 ttgctgtcgt cgatgaacac cacgccgttt ttctcgccga ttttttccac gcggtgcggc
    16861 aggccttgaa aggttttgac gtgttcgagc aattcatttc gcggcaaacc gacggcttcg
    16921 cacaaggcga cggcagccat cacgttggcg gcattgtgca ggccttgcaa cggaatgtct
    16981 tgcgtggcaa tcaaatcttc attgccttgt ttcaggcggc ctgtctcgcg ttccaaccaa
    17041 aaatcggctt cgtgttccaa cgaaaaccat tttacctcgc gcccggcgcg cttcatggca
    17101 caacagaacg tatcgtccgc attcaaaacc tgaacgccat cgccacggaa aatcttgtcc
    17161 ttggtatgcg cgtagtcgag caggtcgtcg tagcggtcga gatggtcttc ggaaatgttc
    17221 agcaccgtcg ccgcagtcgg gcgcaggctt tcggtgtttt ccagttggaa gctggaaagc
    17281 tccaacaccc acacgtccgc ctttttgcct tcacgctgca attccgcctc taaaaccggc
    17341 gtgccgatat tgcccgcgat aacggtatcc agcccgcact tgatgcagag atagccgact
    17401 aggctcgtta ccgtggtttt gccgttgctg ccggtaatcg caattacctt gtcgccccgg
    17461 cggttcacaa tgtccgccag caattcgatg tcgcccaaca cgcgtccgcc gttttgcttg
    17521 aacgcctcaa tatccggctg ccgctcgctg atgccggggc tgagtgccag aatatcgaaa
    17581 ccgttgtcca gcgcatcttt cagacggccc gtgtaaaaca ccaacccgtc aaacatctta
    17641 ccgatttgcg acacgcgttc cggcttcagc tccgcatcat acgcagcaac ctccgcgccg
    17701 tttttgcgca ggtaggcaat catggaaata cccgtaccgc cgagtccggc gacgaggatt
    17761 tttttgtttt gaaaagtcat tttggtttgt cctaaaacaa atcatattga gcaggagatg
    17821 tccgcccctg cccaagccgc tttcagacgg catcgcgagc tgttcaataa cccgctttca
    17881 ggcgttggtc attgtcgcag ctgtcttggt atccgttttg acaagccttg ccaagccatt
    17941 cttgtgcaag ggcgcggtct tggcgcacgc cgcgtctttc ggcatacatc acgcccaaat
    18001 tgttttgggc tgctgcattt ccctgtgctg ccgcctgcaa gttttcccga aaatccgata
    18061 cgtcatccgc ccacaccgct tggttcaagc ccaaggcaat cagggcggcg gcaagccatt
    18121 tgactgtctg tttcatggtt ttacttctgt tttagtataa gccgccgtta acgatagggc
    18181 tgggcggatt gtcgccgcag gtttattgcg cgttcaaatg ccgtctgaaa ggtgttcaga
    18241 cggcataggt tcagcggatt ttgagggtac tcaaaccgat caacaccaag acgatggtaa
    18301 taatccaaaa gcggacgacg acttgggttt ctttccagcc tttttgttcg tagtggtgat
    18361 ggatgggcgc catcaggaag atgcgttttt tggttttctt ataccagccg acctgaagca
    18421 taacggatac ggcttctacg acaaataatc cgcccataat gacgaggaca aactcttggc
    18481 ggacgatgac ggcgacggta ccgagcgcgg cacccaatgc caatgcaccg acatcgccca
    18541 taaagacttg cgcgggatag gcgttaaacc acaagaaacc gaggcacgcg ccgcacatgg
    18601 cggtacagaa aatcaccact tcgtttgcgc cggcaacgta aggtaattgc aggtattggg
    18661 caaattgtga gtggccgctg gcataggcga agatggcgag gccggcggca acgaggacga
    18721 cggggaaggt cgcaaggccg tccaagccgt cggtgaggtt gacggcattg gatgtgccga
    18781 cgatggtcag gtaagacaac accaaaaagc cgaccacgcc cagcggcagg gcgatttgtt
    18841 tgaagaacgg gacaatcaaa atattgttgg cggaattggc ggcaaggtaa aacaatgcca
    18901 aaccggcgat aatggcaacg cttgactgcc acaccatttt gaatttggcg gacacgccgt
    18961 tggggtcttt atagacgact ttgcgccagt cgtcgtaaaa accgagtgcg cccgtggcga
    19021 gcaatacgcc caagagaatc cagatatacg ggtttgccca gttgccccac aacagggtgg
    19081 acacggtaat ggcggtcaga atcagcgaac cgcccatcgt cggcgtgccg tttttgacga
    19141 ggtgggtttg cggaccgtcg gtacgcactg cctgcccgca tttgagcgcg gtcagcctgc
    19201 gtatcgtcca cgggccgaac atcagggaaa acgccaaggc ggtcaacgcc gccatgacgg
    19261 cgcggaatgt ggtgtattga aaaatattca gaccggttaa ccagttgctg aaatgtgcga
    19321 gccataaaaa catggggctt cctttttttg tttttgtcgt tacatgggct tgtgcccgtt
    19381 tgaggttggc ttgccgcccg gcaaggtttc ggacgacttt tgcagattaa cgtgcagaga
    19441 ctttcccaat cacgcaaatc gggtaactcc ccggattttt gccgccaccc aagtctaaac
    19501 ataaatcttc ataaagatac gcctgcgtct gcattccggc ataaaacgcg ccgaccagcg
    19561 cggcaatcaa caataaggct ttaaaaaaac gtcgactttt cattttcact tatcctccaa
    19621 tgcctcgacc acttcttcca tctgcataaa gcgcgaacct ttcaccaaca cggtggcgcg
    19681 ttcgggcaaa tcgtggcgca acacttgaat caacgggtct ttggcggcga accacaaacc
    19741 gtccgcgcca aatttttccg ccgcttcgac gctgttgtcg ccgacaaaat aagccgcttc
    19801 gatgccttgg tcgcgggcgt acgcgccgac ttcggcgtgc atggcggcgg cttcgtcctc
    19861 acccagttcg cccatatcgc ccatcacgaa aatacgcggc gcaggcatac gcgccaacac
    19921 gtcaaccgca gctttcatgc tgtcagggtt cgcattataa gtatcgtcaa tcagggttgc
    19981 gcccttgatt ccggatttga cgttcagacg gcctttgata ttgctgaagc ctttcaaacc
    20041 ttccgccaca tcgttcaaac tcaaacctgc agccaaagac agcgcggcgg cggcggcggc
    20101 gttgtggaca ttgtggcggc cgggaacggg cagaaccacg gctgcgcact cgttgccgca
    20161 caccaaatca aattcgcacg acaacggttt cagcacgata ttttccgcgt ggacatcgcc
    20221 gctatcgatg ccgaaagtgc gcgtattcaa attaagcgtt gccgttttga agacagccat
    20281 attggcatct tcttgaggaa tcagcgccat gccgtctgaa cataagcctt gataaatctc
    20341 gcttttcgct ttggcaatat cgcccactcc gtcgaaaccg cagccgacat gggcgcgcat
    20401 ggcgttgttg accaatgcgg catcgggttt ggcgatttgt gtcaaaaccg ccagttcgcc
    20461 aaaatggttc atacccattt caatcacggc atagcggtgt ttttcgttta atttcaacaa
    20521 agtcaacggc aatccgatgt ggttgttgaa gttgcctgcc gtcgccaaaa cggcattatc
    20581 gccgaaacgg cggcgcaata ccgcagccaa catttccttc accgtcgtct tgccgcccga
    20641 gccggtaata ccgaacacaa acgggttcac attctcgcgc cacgccttcg ccaacatttg
    20701 caacgcggta agcgtgtcat cgactttcaa cgcgccatcc attgcaacgc aatcttcgcg
    20761 cgaaaccaca accgccgccg cacccgcagc caatacgtct tcaacaaaat catgcgcatc
    20821 aaaccgcccg cccgccaatg cgaaaaacac atcgcccgcg cggatgtcgc ggctgtcggt
    20881 tacgatgcgc gacacgggtt tgctttcaga cggcatcgga agcttgaggg cttggcagat
    20941 gaaatttagg tccagtggtt tcatatttac tttcgttaat attcgggcgg cggacacatc
    21001 ggtagcggct gattttttta tcgcctgttt tgctgtggta aaacacagat tattttccca
    21061 ttctcattcg gcattttttc tgtacgtatc attttttaga cgtattttta gtcgatttgc
    21121 cttttcccgc ataccacggc gcggggtcgt cgggcagtcc gtcgataaag gcaaggttat
    21181 tgccttcgcc ctgcacatcg ggaacattcc cccaaaaatc atagccgtca tcgggcaact
    21241 cgtcggtttc gatacccgtc caactgccga atccgcgtaa aaaattaaac gcgctgcgcc
    21301 tcactttaat catttcgcgc cgcgtatcat cggacgaaat tttaaccagc ggcacgccgt
    21361 agctttgacg cttccacgca ccatgtgtca acgttcgctc gccgccttta ccgacatgca
    21421 tcaaaccgtg gtcggaaaag taaaccaaag accagctttc tttattttca ttcaatatct
    21481 taactgtatc ttctaaaaat ttatcggttt gcgcgatggt ggaaacatag caggatattt
    21541 tttcagtttg atactgaaac cgccgcgcat ccttatccaa acgtgtgcaa aaatcactgt
    21601 gcgaacccat caggtgcatc acaatcagcc gaggcttcgt gcctgcattt cctatcaaaa
    21661 cccgtttgaa cgccggcaac aaaaggcggt cgctcaaccc cgcgcttttg ccataatcac
    21721 ccctttgggt aaaccacgga taatcgctgc gtagggcata ggtggaaatt tcgttggcaa
    21781 aatgccccaa cattccttga ttagacagcc acgccgtccg aaaacccgcc tgcttcgcca
    21841 agctgacgat gttattgttc ggttctcccg gcagccccaa agtctgcggc agcgaaagat
    21901 tcgtcgcgtg ggcggtcgat tggtaaccgt ttatcaacag ccctttggtc tgactcaaaa
    21961 aaggcgtatc gggcaatggg aaaccgtaaa cattcatata atccgaacgc gcgctctcac
    22021 cgataaccac aacataattt ttatacttgg gcgcaacatg acggatatgc catgttgacg
    22081 ctttttttgc tgcttccaaa atgtgggcgc gcttggcggc atattgtgcc ggcgcggaag
    22141 ccaaatcgta atacaggtcg aaaatattca acaacaggcc ggcatcaggt tctcgcaaat
    22201 ctttatcgct ggcgatttta tccatcaccg cgcaggacaa aatcaaagtc aataatatag
    22261 tcagccatat tttgctgcgg cgtttatagt tttttacgtc agcaaatacc cccccggtac
    22321 acaatatctc caaactgtca agccaaaaat gaaaaataat gcctgcacaa aataaagcga
    22381 cccgggaaga ttgccgacaa attcacgcgc ctcggcagga ttgctttcca atatcgaacc
    22441 gactatctga taagacggcg caccatacag ccagccgacc ggcaaatata gggcggttgt
    22501 gccgacgtaa atcagtaaaa caactgaaga aacgcgcggg aaaccgcgtg ccaataataa
    22561 taaaaataca accgaagcca aagcacccac ggcagaataa cggtagccgt attcatattc
    22621 caagtgataa cctgatgcga tggccgcgcc aaaacaaaag gctaaaagcg cgcaaatgcc
    22681 caatgtattt tttaatgtct gatccatatt ttttattcgg gcggattgtt tcatacgatg
    22741 ccgtccgaat atcccattat ttacgagtta acaaagcctg tccgacgatt tcaagatcgg
    22801 aaaaacggtg cttcacgcct tgtacatcct gatagttttc atgccctttg ccggcaatca
    22861 ggatgatgtc gtttgcggcg gcttgttcaa ccgcataacg gatggcggcg gcacggtcgg
    22921 cttcgacgca ttcgggcgcg ggaacggcag gcaggatgtc gttgatgatg tcgtgcggat
    22981 tttccaaacg cgggttgtcg ctggtgacga cgactttatc cgcgccctgt acggctgccg
    23041 cgcccatcag cgggcgtttg ccgcgatcgc ggttaccgcc gcaaccgaat acgcaccaca
    23101 aagccgcacc ctgcggtttg atttcctgca aggtggcgag tgctttttcc aatgcgtcgg
    23161 gcgtatgggc ataatcgaca acgaccaaag gtttgccgct gttcatgatg cagtccatgc
    23221 gccctgaagc gggacggatt tttgccagca catccaatac tttatcaagc ggatagccgt
    23281 tggcgcacag caaggcgatg caggcggcga ggttttgcgc gttgaaccgt ccgagcaggc
    23341 gcgtgcggca tttcccttcg ccccacgggg tttggaatac ggctgctatg ccgtctgaag
    23401 aggcggtgaa atcgataatg cggatgtcgg cgtgttcgct gaagccgtag ctataaacgg
    23461 ccaaatcggg acagtctttt ttcagacgac ctacgagttc cgcgccgtat tcgtcatcca
    23521 cgttgatgac tgcgtgtttc aagccgtgcc agtaaaacag gcgcgacttg atggcaccgt
    23581 aggcttccat cgtgccgtgg tagtcgaggt ggtcgcgggt gaggttggta aagattgcgc
    23641 tgcggaatga cacgccgttg acgcgcgact ggtcaagccc gtggctggag acttccatcg
    23701 cggcgactgt tgcgccttgt tgacggaaac ggtagagcag ggtttggaca tcgacggggg
    23761 cgggtgtggt atgcgtggtt tcttccaatg caccccaaaa gccgttgccg accgtgccga
    23821 caatggcggt tttttcgccc aacaaatcgg cagcttgcgc cagccattgc gtgatggagg
    23881 ttttgccgtt ggttccggtt acgccccaga atttgagacc gtctgaaacg ttgccgtaaa
    23941 cttgcgccgc caatatgccg gcacggtgtt tcaaatcttt gatgccttga ttggggactt
    24001 tccattcggg attccacgca aatttgccgt cgtcgtccca aaaaacaaaa gccgcgccgt
    24061 tggcaacggc ggcggggata taactgcgtc cgtcggcata ttcgcccgga caggcaacga
    24121 aaatatcgcc ttgtttgatt tggcggctgt ctgaatgcaa caaacgccct gccgcgtttg
    24181 cacacgacag ggtcgggatg ccggtttcag ccaaaggggt taacttgctg aacataaaac
    24241 aatctcgttg atactcggat taagacggtg ttttgacggc tgcggcggtc aggggcttgg
    24301 tcggggaaat gcccaagatg ttcaggctgc cgcccataat ttttttgaag ggcggccctg
    24361 ccactacgcc gccgtaataa ccgtgggcag tcggttcgtc aatggttacc gccacaatca
    24421 cacgggggtt tttggcgggg gcaaaaccga taaaggtagc gatgtgtttg ttgtcggcat
    24481 aacgcccgtt gacgaacttg cgcgccgtgc cggttttcgc gccgacatcg aaaccgtcca
    24541 ccgcacccgc cgtaccggtg ccgcccggct cggttacgga aaccatcaga ttgcgtacct
    24601 cgcgcgcggt cgattctttg aatatgcgtt tgccttgcgg cgcaaccgcc tgtttttcaa
    24661 agctgaccgg cagcaaaacg ccgtcgtgcg tcagtgcggt ataggcgcgc gccaattgca
    24721 gcaggctcaa ttgcaggccg taaccgaaag acatcgtcgc ctgttcaatg ggccgccacc
    24781 tgcgccaatt tctcaacaaa cctgcagttt cgcccggaaa gcccgagtgc atacgcacac
    24841 cgatgcccaa ctcatgatag aagtcataca tttcttcggc accgaaacgc gcagacagtt
    24901 tgcttgtgcc gacgttggac gatttctgca tgatgccgcg cacatccaaa gaggggtaaa
    24961 catgggtatc gcgcacggga gacggtccga ttttataagg ctgcgtattc agccgttcgt
    25021 tcaaatcggt tttgcccgca tccaatgcct tcgcaatcac aaacggtttg attgccgaac
    25081 cgggttcgat catatcggtt acggcacggt tgcgccgctg ttcgctgtct gcccggccgg
    25141 gcctgttggg atcgtaggcg ggcgtattgg ccaaggcgag gatttccccc gtgcgggcat
    25201 ccaaaaccac caccgttccg gcttttgcct gatggtattc gaccgccttg ttcaactctt
    25261 cataggccaa ggtctgaatc ctctgatcga gggaaaggat gatgtctttg ccgtttttcg
    25321 gggctttatt gcgcggggag tccaagctgt ccacaatatt gccctgccgg tcccgcaaaa
    25381 cgacttccgc gccgtcttcg ccatgcaggc tgtcttcaag cgaaagttcc aaaccttcct
    25441 gacctttgcc gtcaatatcg gtaaatccga tgacgtgtgc aaacaggttg cccatcgggt
    25501 aatggcgttt taattctttt tcaaatacaa agttttccaa acccaaggct ttgacctctt
    25561 cggcaacctt gggatcgagc tgccgcttaa tccagataaa cgacttgcct ttctgttcga
    25621 gcttgttcct caaaacatca accggcacat cgacaagctc ggacaggcgt tccaattgtg
    25681 cggcagacgg catttccttc atctctttag gcacggcaaa cagggactcc gtcggcgcac
    25741 tcaacgccaa aaccgcaccg ttccggtccg aaaccgtacc gcgtgtagcc ggcaatgttt
    25801 gagtccgcac aatccggttg tcgccctgtt ctttcaaaaa gttatacgtt accgtctgca
    25861 gatacagtcc gcgggcaatc agaccggcaa acaagaccgc tattgccatc aggacgaagc
    25921 taatccgtcc gttactggtc atcggctttt tgacctgctc ttctttgggc agcatccgag
    25981 gcttatattc gctcttaatc aacattttta cttctcgtta ttattatcct gacgcaggaa
    26041 tccgattccg gcacacaggc tgcttctatc tttgatgctc caccataaag gtattgcccg
    26101 aaaccggcgg atggaggttt tgtttttctg ccgccgccct gatcgcttcg tggttcgcca
    26161 aacgcgcctg ttgcagcctc atttgcgcat aatcctgctc caaggcgatt tcctgttttt
    26221 tcgccttgtc caaagctgtg aagttgagcc tgtactggtt ttgctgcatc acaacggaaa
    26281 aagcggaaac gcacaccgca agcagcagaa ggaaattcaa tttgttcatt gccattcaga
    26341 cgtttttctc tgtgattgtt ccggtatcgg accggcagtc cgctccgcca cacgcaaaac
    26401 cgcacttctc gccctcggat tggcggcaat ttccgcctca cccggcttta atgccctgcc
    26461 cacgattttc aggggcggct cgggcaaatc cgcttccctg accgccgccc agcgcggcag
    26521 gggcgcgtgt tgcgaatatt ttttgacaaa ctgcttcaca atgcggtctt ccaacgaatg
    26581 gaaagcaatg accgccaaac gtccgccctc tttcagacga cacatgacct gcggcaatac
    26641 tgcccctact tcttcaagct cgcggttaat aaagatgcgg accgcctgga aggtgcgcgt
    26701 cgcaggatcc tgcccccgct cgcgagtacg gacgttttgt gccacgatct gcgccagctt
    26761 gcgggttgta tcgattggac tttccgcccg ttgcgcaaca atggcgcgcg caatctggcg
    26821 gctaaaccgc tcttcaccat aattcttgat tacctcgtgc aaatcctgtt ccgacgcaac
    26881 cgctatccac tctgcggcag acataccgcg cgtcgtatcc atacgcatat ccaaaggggc
    26941 atcgaaacga aagctgaaac cgcggctgcc gtcatcgatt tgcggagacg aaatccccaa
    27001 atcaaacagc gcaccgtcca ccttgccgat acccaaaccg tccaatgccg tctgaaacga
    27061 agcaaaaccg ccatgcacga caccgacccg tttgtccgaa cgcgccagct cttctgccac
    27121 agcaatcgcc tgagggtctt tgtcgaaaac aatcaaccgc cccgcatcgc ccaaacgcga
    27181 caaaatcagc cgggaatgcc ctcccctgcc gaacgtaccg tccacataga caccgtcttc
    27241 gcgcacggca agcgcatcca ccgcctcatt cagcaagacc gtgatatgcc ggtaactttc
    27301 tgctccactc acaattgcaa atccgtctga cccaactgga aggcaagttc gtcaggatcg
    27361 tcatccaaag cctgaaccat ctcagcctcc cactgctcgc gaccccaaag ctccaaacgg
    27421 ttggcacgac cgaccaaaac gacttcacgg tcgaaatcca ccctcttcct cagtccggca
    27481 ggaaccagca cccggccggc gctgtcccat tccaaaattt ccgcgttatg cagcagaaga
    27541 ttttgaaacc gccgtaaaac agggttatcc gccactttta agtttaaaag ttgcgccgca
    27601 accttttccc actccgcaac agggtacatc aacagcttgt gtttcgactc gagcgttacc
    27661 actacggcag gcgtatagag gcgcgacaga atgtcacgga atttggcagg aacagccaac
    27721 cgccccttac tgtcgatgct taattcgtgt gcgccgccga acatgacatg tcccaagccg
    27781 aaatcaaaat cacaagggta aaagagacac tttgccccac aattccccac caatcgacac
    27841 tataagaaat tttaaacact cggtcaaatc agggcatgaa aactcattaa catatctgaa
    27901 atttttattc cttttaaaaa caataagata aaaaatgacg acaacggcac ggcgggtgcg
    27961 gtacagaata atcgaaccaa taaacaacta tatatgatta atttaataat ataaacacaa
    28021 tatatagtat taagataaag ccatgacagc acccgtacca acgtgtaata tgtcgggaaa
    28081 tccaataaat ttacacaagc taacacttat catgcccctc cctctcccct ctcccgaagc
    28141 acggcaatcc tcgctcaaac tgcaaaccct cattgccgaa aaaatcggca aacacggcaa
    28201 ctggattcca ttttcacgtt ttatggaatt ggttttatac gctccgcaat acggctacta
    28261 caccggcggc agccataaaa tcggcaatac cggggatttt attaccgcac cgaccctcac
    28321 ctctctgttt gcacagacac tggcacgcca acttcaagaa cttctatccc aaacggcggg
    28381 caatatctat gaattcggcg cgggaaccgg acagcttgcc gccgatttgt tgggcagcat
    28441 ttcggacggc atcagccgtt actatattat tgaaatatcg ccggagctgg cagcacgtca
    28501 gaaaaacctg attcaagccc gcgcaccgga agcatctcaa aaagttgtcc acttgaccgc
    28561 acttcccgaa gcgtttgacg gcatcatcat cggcaacgaa gtactcgatg ccatgcctgt
    28621 cgaaatcgtc cgtaaaaatg aaggcggctc attcgagcat gtcggcgttt gcctagataa
    28681 tgaccgtttt acctattcgg cacgaccgct gcacgactta cagctatctg ccttggcttc
    28741 cctctatttt cctcaaacag attatcccta caccagcgaa ctacatccgc aacaatatgc
    28801 ctttatccgc acccttgcct ccagactcga acacggctgc atgatattca tcgactacgg
    28861 ttttgatgca gcgcagtatt accaccctca acgcaatcaa ggtactctga tcggacacta
    28921 ccgacatcac gttatccaca atccttttga cttcatcgga ttggccgacc tgaccgcgca
    28981 tgtcaacttt accgacattg cccaagccgg gacggatgcc ggattagatt tgataggtta
    29041 ccttccccaa tcccatttct tattgaacct gggcattacc gagctattgg cacagacggg
    29101 gaaaacgaat tcggcagcct atatccgtga agctgctgcc gttcagaaac tgattgacca
    29161 gcatgaaatg ggcgaactgt ttaaagtcat cgcattcgga aaaaatatcg gcatcgactg
    29221 ggcaggattc cgcttcggcg acatctgcca caaactctaa ccctcatgcc gcctgaatcc
    29281 gcttcagacg gcataaactt tttaacattt aaaaacagtc aactaattca aaattaaaaa
    29341 atacggcttg tcaaaaaaac agaaaaacat ataatagcgt cttcacgaaa cggcgaatta
    29401 gctcagtcgg ttagagcaga ggaatcataa tccttgtgtc cggggttcga gtccctgatt
    29461 cgccaccaaa ttttcggggg tatagctcag ttggtagagc gcttgcatgg catgcaagag
    29521 gtcagcggtt cgatcccgct tacctccacc agataaaaaa gcacagaccg taaaaaggta
    29581 tgtgcttttt tattacctga ttaccggcaa ataaagaata aaccactgcc ttcaaaacag
    29641 gcaatcgact ttaaacctat cgccccgcct gtcctgattt tatagtgaat taaatttaaa
    29701 ccggtacagc gttggctcgc cttgccgtac tatctgtact gtctgcggct tcgtcgcctt
    29761 gtcctgattt ttgttaattc actatatcag cccgccagac aaacccgacc cgaataatgt
    29821 cttcaggtcg ggtttatggt ttcattccca acttatccag cccgacaacc acaatattat
    29881 gatgacgatg ccgaaaacaa tgcggtaata ggcaaacggg atatagtttt tcttggaaac
    29941 aaacttcagc agtgctttga ccgctactaa accggaaaca aaagcggcaa taaagcctat
    30001 cagaatcaaa ccgacatcat gcagggtgaa aaaccggtaa tgtttcagga catcataagc
    30061 cgttgcggca accatcatcg gaacggccaa gaaaaatgaa aactccgttg ccgttttccg
    30121 ctcgattccc caaagcatcc cgcccataat cgtactgccc gagcgggatg ttcccggaat
    30181 caaggcaaac acctgcgcga taccgatcat caaggcatca atcggacgca atgcatcaac
    30241 atcggcaatt ttaggctctg ctcggctttg gcgtttctcc acccacaaaa taaaaaaacc
    30301 gcccaaaacc agcatgactg caacactcaa ggggttaaac agatactctt tgatttgttt
    30361 gccgaacaac agccccatca cggcggcagg tataaaagca atggcaagat tgaggacgaa
    30421 acggttggct ttccggtctt ttcctaagcc gtgcaacaca ttgctgaaac gctgccggta
    30481 ttcaaacact actgccaaaa ccgcaccaag ctggatggca atttcaaaaa ccttgtggtt
    30541 gctgtgaaaa ccgaccaagt taccgaacac aatcaaatgt ccggtgctgg aaataggtaa
    30601 aaattcggta aaaccttcta ccaagcccat catcagggct ttcaggacaa tcagaaaatc
    30661 cattgcttgg tttctttcaa atgcgcgggg atgcggttat ttttgcacag gatggtttga
    30721 cagtttgcgc tccgccctga ctttggcaac caattcttta atcgtatgaa cgccgccgtc
    30781 aaagccgtta ttgaagataa cgcggtattt tccgccgacg acaacggtcg gcgtgctgtc
    30841 gatgcggtat tgttccgtca gtttctgcat ttttaatgcg gcggcggcag cttcggggga
    30901 atcataggcg cgcatcagtt ttttgccgtc aaagcctttt tgagacaaag cccattttcc
    30961 ggcaacagcc ctgttttcca agcggatttt ttgttcgtaa actgctttaa acacggcagg
    31021 gtttgcctga tatttcaaac ccgacaaatt gacggcagcc gccattcttg ccagaccgag
    31081 catttcaagc tgccagacca cgtgctccgt cctcaaatag gcatcagacg gcaatgcctt
    31141 gcccagtttc aataacaaag gatcgaaatg atggcaatgt acgcagaaat agccgaaaaa
    31201 ttccaaaacc tcaattttac ccgactgttc ttgaggaatg ggtttatcca acacaagata
    31261 gtcttcccct tccgtcaggg catatgcctg cgcggacaac actgccgaca gcagcagcgg
    31321 caacagatgt ttgagcttca taattatttg ctttcgatag aacggatcag gctggcgact
    31381 tcatgttttt tcaactcgtc ctgcattttt ttcaccgcat cggcagacat attgccgctt
    31441 tgcacccggt aaagcgtttt atgtcccgcc tgataaccga ccaccttgga agatatgccc
    31501 aagattgcca gtttggcacg ctgcccttcc gcgctccggc ggtcggcata cgcgcccatt
    31561 tgcagataat gcgttgcttc cgccttgtcg gacgttttca ttttctgcac ttctttggcg
    31621 gcggcactgc gcgctttttc gatgctgccg ctgttgagga tttgttccgg ggtcggtttg
    31681 ggtgcaactt tttccttttc cgcctttttc tcttcttttg gagctttttt ctctgtttct
    31741 ttagacggtt ttaccgcttg ttttttaacc gtttcggcat ctttcttctg cgctttttcc
    31801 ctgacggttt gttcacgctc ttccgtcagc actttcttac gcactgcctg tccgtccggc
    31861 tcttcccgtt ccggctcgcc cgccttttct tcaacctcgt cggctttatc ggcagctttt
    31921 tccgcatccg actgctctgc ctctgtcgca gcatccggtt cggacaaggc gttttgatcg
    31981 gccggttcag gttggatgtc ttccttaggc tggtttttcg gtttcaggat ttccgtttct
    32041 gcaggctgct tcgacggaac cgggattttg aacgcatttt gaccgctctg gttcagataa
    32101 aacaaaatac cggcaataat gaccgtcgcc agtatcaaac cgaagaaaaa accggacaga
    32161 ccttttccgg attgggaaaa tttgttcata aacatacctt aatgtgtttc agacggcatt
    32221 aacgccgttt gcttacgggc ggatattcta acaatatcgc catatttggg caaaacctgc
    32281 ttccattccc gttcctataa agcgcgacgg aaacctatgg ctttcatgac gtggcttctg
    32341 ccgacttctt cgtcgcccgc caaatccgcc aatgtacgcg ccacgcgcat aatgcggtgg
    32401 aagctgcggg cggaaaggga gagtttttcc agcaggccgc ccaatgcttc ctgcgcttct
    32461 ttttgaatgc gggcttggga atcgagttcg ctgacgctca aagcggcatt cactttgcct
    32521 tgccgtgcgt attgtttgtc tctggcggcg ataacgcgtt ccaaaacgga cgcgctgctt
    32581 tcccctgctt cctgctgcat cagttcggcg gcggacaggc tcgggacttc gatggtcaaa
    32641 tcgatgcggt cgagcagcgg gccggaaatc ttgctgcggt aacgcgcgac gctttcgggc
    32701 gtgcaacggc agggtttgat gggatgcccg agataaccgc aaggacaagg gttcatggcg
    32761 gcaacaagtt ggaatttggc aggatagacg gcttggcgcg ccgcgcggga aatgtggatt
    32821 tcgccgtttt ccaacggttc gcgcaaaact tctaaaactt tgcggtcgaa ctcgggcagc
    32881 tcgtccaaaa acaatacgcc gtggtgcgcc aatgaaattt cgcccgggcg cggatccgaa
    32941 ccgccgccga ccatagccgc cgcgctggcg ctgtgatgcg gactgcggaa aggacggttg
    33001 ctgtcgagtt gttgttggtg gttgggcagg agcgaacgca atgcccaaac ttctaccaat
    33061 tcgtcttcgg tcagcggcgg caggatgccg ggcagccgtt gggagagcat agacttgccc
    33121 gttcccggcg gacccatcat caagaggctg tgtccgcctg cggcagcgat ttccaaagca
    33181 aggcgcgcgg tgtgctgacc tttcacatcg cacaaatcag gttgtccgcc atgttcagac
    33241 ggcatttgcg gtacggaaca ttcggtttgc gccaaaggtt cgatgccgtt caaatgggcg
    33301 gcgacttcac ccaaagagcg cgcgccgtaa acggtaatgc cgcgcatcac ggcggcttgt
    33361 cctgcgtttt cttcaggcaa aacaaatgca cgttttgcct gcataccctg ccacgccatc
    33421 gccaacgcgc cgcgcacggg gcgcaacagc cccgacagtg ccaattcccc cgcaaactcg
    33481 tattccgcca gtttttcggg cgcaacctgc cccgatgcgg caaggatgcc gattgcaatc
    33541 ggcaaatcga aacgccccga ctctttgggc aggtcggcgg gggcgaggtt gacggtgatt
    33601 tttttggcgg ggaattcaaa accgctttga ataatggcgg cacggacgcg gtcgcgactt
    33661 tcctttactt ccgtatcggg cagcccgacg atgttgaaat gcggcaggcc gttggcaagg
    33721 tgggcttcca cttcgaccaa cggcgcattc ataccactca aggcgcggct gtaaaccaag
    33781 gcaagcgaca tatttcagac ggccttattc gccggcttcg gtttgctgcc tgatttcggc
    33841 gacggcttct tcggcagcgg cttcagccgc ttccaatgct gcccgttcgg gattttgcgc
    33901 ggcttcgagt tttgccaaac gcgcttccaa agccgccagt ttggtacggg ttttgattaa
    33961 aacctgctgc tggatgtcga attcttcgcg cgtaaccaga tccatacggt tgaacgcgcc
    34021 gcccagcatc gccttaatat ttttttccac atctttggca gggctgttgg cgatggtttc
    34081 gctgattttc gagccgactt cctcaaaaag ctgcttgccg aacataatct gtatccttcc
    34141 tgaacatatc aaattcaatc ggctattgta taaggaaaaa tgccgtctga aaacgggcgg
    34201 cggataatcg gcaaaacata ccgcgcctcc tttgcggttg ctaaattaca cctcaaaaca
    34261 cctgccgcct gaaaacggat ttcatattcc gcgcaacgcc cgattacaag acactacaaa
    34321 acaatatgct gttttaaatg atttttccga cgcgcatcgt ttcaaactcg gctttttaag
    34381 ccattaagtg ctttgcaaac aacagaaatt gggttatgct gaaacggatt atttacaatt
    34441 tcatatagtt ttattacata tcttattgtg attgaagata atttatccga atcccccttt
    34501 cgggtatccg gattttccgt tgtactttta ttagaaaaac atttcaggcg caagttgctt
    34561 gcaatttcaa agcctgtaat tctgaagttt tagacgagga atagcacatg aatcccatgt
    34621 acattacttt tgcaatctat ctggttgcag tccttctcat cgggcttgcc gcctattttt
    34681 ctacgcgcaa tttcgacgat tacattttgg gcgggcgcag cctgggcccg tttgttaccg
    34741 cgatgtcggc aggcgcgtcc gatatgtccg gctggctgct gatgggtctg ccgggcgcga
    34801 tttacctgag cggtttgaat gaggcttgga ttgccatcgg cctcttggtc ggcgcgtatt
    34861 tcaactggct cttggtggcg ggccgcctgc gcgtgcatac cgaatacgcc aacaatgcgc
    34921 tgacgctgcc ggattatttc ttccaccgct tcggcgcggg cggacacttg atgaaagtgg
    34981 tttccgcact gattatcctg ttcttcttca cgatttattg cgcctcgggc attgtggcgg
    35041 gcgcaaccct gttccaaagc ctgtttgaag gtatgactta caatcaggca atgtggctgg
    35101 gcgcgggcgc gaccatcgcc tataccttct tgggcggctt cttggcggtc agctggacgg
    35161 atacgttgca ggcttctttg atgattttcg cgctgatttt aacgcctgtg atggtctatc
    35221 tgggcttggg cggcgcggaa cagatgtctg ccgcgattca aaacgtcgcc gcaggcacgg
    35281 gcaaagaata cggcagcctg tttgccggta cgaccgtcat cggcatcatt tccaccgccg
    35341 cctggggctt gggctatttc ggacagccgc acattttggc gcgctttatg gcggccgaaa
    35401 gcgcgaaatc gctggtatcc gcacgccgca tcggtatgac ttggatggcg ttgtgccttg
    35461 cgggcgcggt agcggtcggt tatttcggca ttgcgtattt cggtgcaaat cccgacaaag
    35521 tttcttctat gagcggcaac cacgaacgca tcttcatcgc gctttccacc ctgctgttca
    35581 acccttggat cgccggcatt attttgagcg cgattttggc ggcggtgatg tccaccctgt
    35641 cctgccagct tttggtttgc tccagtgcga ttaccgaaga cttctacaaa ggctttttgc
    35701 gtaaaaacgc gcaacagtcg gaactggtat gggtcggccg cctgatggtc ttggcgattg
    35761 ccgtgatttc catcctgatt gcttccgatc cgaacagcaa agtattggga ctggtgtctt
    35821 atgcgtgggc aggtttcggt gcggcattcg gcccgattgt gattctgtcc gtattgtgga
    35881 aacgcatcac tgcctacggc gcgttgtcgg gtatggttgc aggtgcctta accgtagtcg
    35941 tatgggcgga atgggtgaaa aaaccggctc aagcggcagg cgaaagcggc ttgttgacga
    36001 tgtacgaaat cgtcccgggc tttattgttt gcttgatcgt tgccgtgttg gtttccctgt
    36061 tcaacaaaga accttcgcgc gaaacccaag aacgctttga aaaagccgac gcagattacc
    36121 gcgccgcccg ataaacccct ttcagacggc acggcatccc ggttcgtgcc gtctgaaccc
    36181 gtttccgggt caaacacaaa taaaccttgc ctgcatacag gccttatttg tgttttaccc
    36241 gtttcatttt caaagaaacg gaagcaccta ccctgcccgc atttgaagcc ttgtatttca
    36301 tctgcggatt tattacgata ttaccgtatt caggccgcac cgatgccgcc tgccccccga
    36361 aaaactttgg agaatccaaa aaatgtttca ttttgcattt ccggcacaaa ctgccctgcg
    36421 ccaagcgata accgatgcct accgccgtaa tgaaatcgaa gccgtacagg atatgttgca
    36481 acgtgcacag atgagcgacg aagagcgcaa cgccgcctcc gagcttgccc gccgtttggt
    36541 tacccaagtc cgcgccggcc gcaccaaagc cggcggcgta gatgcgctga tgcacgagtt
    36601 ttcactctcc agcgaagaag gcatcgcgct gatgtgtctg gcagaagccc tgctgcgtat
    36661 ccccgacaac gccacgcgcg accgcctgat tgccgacaag atttcagacg gcaactggaa
    36721 aagccatttg aacaacagcc cttccctctt cgtcaacgcc gccgcctggg gcctgctgat
    36781 taccggcaaa ctgaccgcca caaacgacag acaaatgagt tccgcactca gccgcctgat
    36841 cagcaaaggc ggcgcaccgc tcatccgcca aggcgtaaat tacgccatgc gcctgttggg
    36901 caaacagttc gtaaccggac agaccattga agaagccctg caaaacggca aagaacgcga
    36961 aaaaatgggc taccgcttct ccttcgatat gttgggcgaa gccgcctaca cccaagccga
    37021 tgccgaccgc tactaccgcg actatgtcga agccatccac gccatcggca aagatgcggc
    37081 aggacaaggc gtttacgaag gcaacggcat ttccgtcaaa ctctccgcca tccatccgcg
    37141 ctactcgcgc gcccaacacg gccgtgtgat gggcgaactg ttgccgcgcc tgaaagagct
    37201 gttccttttg ggtaaaaaat acgatatcgg tatcaacatc gatgccgaag aagccaaccg
    37261 tctggagctg tctttggatt tgatggaggc actcgtttcc gatcccgatt tggcaggcta
    37321 caaaggtatc ggtttcgttg ttcaagccta ccaaaaacgt tgtccgttcg ttatcgacta
    37381 cctgatcgac cttgcccgcc gcaacaacca aaaactaatg atccgcctcg tcaaaggcgc
    37441 gtattgggac agcgaaatca aatgggcgca agtggacggc ttgaacggct atccgaccta
    37501 cacccgcaaa gtccacaccg acatctccta cctcgcctgc gcgcgcaaac tgctttccgc
    37561 gcaagacgcg gtatttccgc aattcgccac ccacaacgcc tacaccttgg gcgcaatcta
    37621 ccaaatgggc aaaggcaaag actttgaaca ccaatgtctg cacggcatgg gcgaaaccct
    37681 gtacgaccaa gtcgtcggcc cgcaaaactt aggccgccgc gtgcgcgtgt acgccccggt
    37741 cggcacacac gaaaccctgc tcgcctactt ggtgcgccgc ctgttggaaa acggcgcgaa
    37801 ctcgtctttc gtcaaccaaa tcgtcgataa aaacatcagc atcgacacgc tcatccgcag
    37861 cccgttcgac accatcgccg aacaaggcat ccacctgcac aacgccctgc cgctgccgcg
    37921 cgatttgtac ggcaaatgcc gtctgaactc gcaaggcgtg gacttgagca acgaaaacgt
    37981 attgcagcag cttcaagaac agatgaacaa agccgccgcg caagacttcc acgccgcatc
    38041 catcgtcaac ggcaaagccc gcgatgtcgg cgaagcgcaa ccgattaaaa accctgccga
    38101 ccacgacgac atcgtcggca cagtcagctt tgccgatgcc gcgcttgccc aagaagcggt
    38161 tggcgcagcc gttgccgcgt tccccgaatg gagtgcgaca cctgccgccg aacgcgccgc
    38221 ctgcctgcgc cgtttcgccg acctgctcga acagcacacc cccgcgctga tgatgcttgc
    38281 cgtgcgcgaa gcaggcaaaa cgctgaacaa cgccattgcc gaagtgcgcg aagccgtcga
    38341 tttctgccgc tactacgcaa acgaagccga acataccctg cctcaagacg caaaagccgt
    38401 cggcgcgatt gtcgccatca gcccgtggaa cttcccgctc gccatcttta ccggcgaagt
    38461 cgtttccgca ttggcggcag gcaacaccgt catcgccaaa cccgccgaac aaaccagcct
    38521 gattgccggt tatgccgttt ccctcatgca cgaagccggc atcccgactt ccgccctgca
    38581 actcgtcctc ggcgcaggcg acgtgggtgc ggcattgacc aacgatgccc gcatcggcgg
    38641 cgtgattttc accggctcga ccgaagtggc gcgcctgatc aacaaagccc ttgccaaacg
    38701 cggcgacaat cccgtcctga ttgccgaaac cggcggacaa aacgccatga ttgtcgattc
    38761 caccgcactt gccgaacaag tctgcgccga cgtattgaac tccgccttcg acagcgcggg
    38821 acaacgctgc tccgccctgc gtatcttgtg cgtccaagaa gacgttgccg accgtatgct
    38881 cgacatgatc aaaggcgcga tggacgaact cgtcgtcggc aaaccgattc agctcactac
    38941 cgatgtcggc cccgtcatcg atgccgaagc acagcaaaac ctgttgaacc acatcaacaa
    39001 aatgaaaggc gttgccaagt cttaccacga agtcaaaacc gctgccgatg tcgattccga
    39061 aaaatccacg ttcgtccgcc ccatcctgtt tgaattgaac aacctcaacg aactgcaacg
    39121 cgaagtcttc ggtcccgtcc tgcacgtcgt ccgctaccgc gccgacaaac tcgacagcgt
    39181 catcgaccaa atcaacagca aaggctacgc cctgacccac ggcgtacaca gccgcatcga
    39241 aggcacagta cgccacatcc gcagccgcat cgaagccggc aacgtttacg tcaaccgcaa
    39301 catcgtcggc gcagtcgtcg gcgtacagcc cttcggcgga cacggtctgt ccggcacagg
    39361 ccccaaagca ggcggttcgt tctacctgca aaaactgacc cgcatccccg aatgggttgc
    39421 cccgaccctg agccaaatcg gacaggcaga cgaagccgca ctcaaacgcc tcgaagcact
    39481 ggttcacaaa ctgccgttca acgccgaaga gaaaaaagcc gcagcggccg ctttgggaca
    39541 cgcccgcatc cgcaccctgc gccgtgccga aaccgtcctt accggaccga ccggcgagcg
    39601 caacagcatc tcatggcacg cgcccaaacg cgtttggata cacggcggca gcacggttca
    39661 agcctttgcc gcactgaccg aacttgccgc ctccggcata caggcagtgg tcgaacccga
    39721 cagccccttg gcttcctaca ctgccgactt ggaaggtctg ctgctggtca acgacaaacc
    39781 cgaaaccgcc ggcatcagcc acgttgccgc cctgtcgcct ttggacagcg cgcgcaaaca
    39841 ggaacttgcc gcccacgacg gcgcactcat ccgcatcctc ccttcggaaa acggactcga
    39901 catcctgcaa gtgtttgaag aaatctcttg cagcgtcaac accacagccg ccggcggcaa
    39961 cgccagcctg atggcggttg ccgactgatt ttaccgaaat ccccgggcgc ggcccgtgaa
    40021 ccaatgccgt ctgaaaacct ttcagacggc atttttataa
//
LOCUS       DAWFKN010000010        38989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFKN010000010 DAWFKN010000000
VERSION     DAWFKN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.010"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGR46_000488"
     CDS             <1..69
                     /locus_tag="WGR46_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0855492.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            87..398
                     /gene="rpsJ"
                     /locus_tag="WGR46_000489"
     CDS             87..398
                     /gene="rpsJ"
                     /locus_tag="WGR46_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0855493.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            689..1741
                     /locus_tag="WGR46_000490"
     CDS             689..1741
                     /locus_tag="WGR46_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ0855494.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            1750..2433
                     /locus_tag="WGR46_000491"
     CDS             1750..2433
                     /locus_tag="WGR46_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ0855495.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            2652..3296
                     /gene="rplC"
                     /locus_tag="WGR46_000492"
     CDS             2652..3296
                     /gene="rplC"
                     /locus_tag="WGR46_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0855496.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            3296..3916
                     /gene="rplD"
                     /locus_tag="WGR46_000493"
     CDS             3296..3916
                     /gene="rplD"
                     /locus_tag="WGR46_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0855497.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            3913..4227
                     /gene="rplW"
                     /locus_tag="WGR46_000494"
     CDS             3913..4227
                     /gene="rplW"
                     /locus_tag="WGR46_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0855498.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            4233..5066
                     /gene="rplB"
                     /locus_tag="WGR46_000495"
     CDS             4233..5066
                     /gene="rplB"
                     /locus_tag="WGR46_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0855499.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            5073..5351
                     /gene="rpsS"
                     /locus_tag="WGR46_000496"
     CDS             5073..5351
                     /gene="rpsS"
                     /locus_tag="WGR46_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0855500.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            5360..5689
                     /gene="rplV"
                     /locus_tag="WGR46_000497"
     CDS             5360..5689
                     /gene="rplV"
                     /locus_tag="WGR46_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0855501.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            5699..6391
                     /gene="rpsC"
                     /locus_tag="WGR46_000498"
     CDS             5699..6391
                     /gene="rpsC"
                     /locus_tag="WGR46_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0855502.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            6375..6791
                     /gene="rplP"
                     /locus_tag="WGR46_000499"
     CDS             6375..6791
                     /gene="rplP"
                     /locus_tag="WGR46_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0855503.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            6791..6982
                     /gene="rpmC"
                     /locus_tag="WGR46_000500"
     CDS             6791..6982
                     /gene="rpmC"
                     /locus_tag="WGR46_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0855504.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGR46_000501"
     CDS             6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGR46_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0855505.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            7467..7835
                     /gene="rplN"
                     /locus_tag="WGR46_000502"
     CDS             7467..7835
                     /gene="rplN"
                     /locus_tag="WGR46_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0855506.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7847..8170
                     /gene="rplX"
                     /locus_tag="WGR46_000503"
     CDS             7847..8170
                     /gene="rplX"
                     /locus_tag="WGR46_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0855507.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            8180..8719
                     /gene="rplE"
                     /locus_tag="WGR46_000504"
     CDS             8180..8719
                     /gene="rplE"
                     /locus_tag="WGR46_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0855508.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            8722..9027
                     /gene="rpsN"
                     /locus_tag="WGR46_000505"
     CDS             8722..9027
                     /gene="rpsN"
                     /locus_tag="WGR46_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0855509.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            9043..9435
                     /gene="rpsH"
                     /locus_tag="WGR46_000506"
     CDS             9043..9435
                     /gene="rpsH"
                     /locus_tag="WGR46_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0855510.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            9449..9982
                     /gene="rplF"
                     /locus_tag="WGR46_000507"
     CDS             9449..9982
                     /gene="rplF"
                     /locus_tag="WGR46_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0855511.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            9996..10349
                     /gene="rplR"
                     /locus_tag="WGR46_000508"
     CDS             9996..10349
                     /gene="rplR"
                     /locus_tag="WGR46_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0855512.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            10368..10886
                     /gene="rpsE"
                     /locus_tag="WGR46_000509"
     CDS             10368..10886
                     /gene="rpsE"
                     /locus_tag="WGR46_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0855513.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            10879..11064
                     /gene="rpmD"
                     /locus_tag="WGR46_000510"
     CDS             10879..11064
                     /gene="rpmD"
                     /locus_tag="WGR46_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0855514.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            11066..11500
                     /gene="rplO"
                     /locus_tag="WGR46_000511"
     CDS             11066..11500
                     /gene="rplO"
                     /locus_tag="WGR46_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0855515.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            11512..12822
                     /gene="secY"
                     /locus_tag="WGR46_000512"
     CDS             11512..12822
                     /gene="secY"
                     /locus_tag="WGR46_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0855516.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            12827..13045
                     /gene="infA"
                     /locus_tag="WGR46_000513"
     CDS             12827..13045
                     /gene="infA"
                     /locus_tag="WGR46_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0855517.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGR46_000514"
     CDS             13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGR46_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0855518.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            13245..13607
                     /gene="rpsM"
                     /locus_tag="WGR46_000515"
     CDS             13245..13607
                     /gene="rpsM"
                     /locus_tag="WGR46_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0855519.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            13627..14022
                     /gene="rpsK"
                     /locus_tag="WGR46_000516"
     CDS             13627..14022
                     /gene="rpsK"
                     /locus_tag="WGR46_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0855520.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            14042..14662
                     /gene="rpsD"
                     /locus_tag="WGR46_000517"
     CDS             14042..14662
                     /gene="rpsD"
                     /locus_tag="WGR46_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0855521.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            14688..15674
                     /gene="rpoA"
                     /locus_tag="WGR46_000518"
     CDS             14688..15674
                     /gene="rpoA"
                     /locus_tag="WGR46_000518"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0855522.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            15698..16066
                     /gene="rplQ"
                     /locus_tag="WGR46_000519"
     CDS             15698..16066
                     /gene="rplQ"
                     /locus_tag="WGR46_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0855523.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            16213..16926
                     /gene="minC"
                     /locus_tag="WGR46_000520"
     CDS             16213..16926
                     /gene="minC"
                     /locus_tag="WGR46_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0855524.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            16955..17770
                     /gene="minD"
                     /locus_tag="WGR46_000521"
     CDS             16955..17770
                     /gene="minD"
                     /locus_tag="WGR46_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0855525.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            17774..18037
                     /gene="minE"
                     /locus_tag="WGR46_000522"
     CDS             17774..18037
                     /gene="minE"
                     /locus_tag="WGR46_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0855526.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            18041..18961
                     /gene="oxyR"
                     /locus_tag="WGR46_000523"
     CDS             18041..18961
                     /gene="oxyR"
                     /locus_tag="WGR46_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0855527.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(18958..19149)
                     /locus_tag="WGR46_000524"
     CDS             complement(18958..19149)
                     /locus_tag="WGR46_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855528.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(19291..22128)
                     /locus_tag="WGR46_000525"
     CDS             complement(19291..22128)
                     /locus_tag="WGR46_000525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0855529.1"
                     /translation="MLDKYNPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTVWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPSEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDETG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGR46_000526"
     CDS             complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGR46_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0855530.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(23215..24471)
                     /locus_tag="WGR46_000527"
     CDS             complement(23215..24471)
                     /locus_tag="WGR46_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0855531.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(24550..25941)
                     /locus_tag="WGR46_000528"
     CDS             complement(24550..25941)
                     /locus_tag="WGR46_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0855532.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGR46_000529"
     CDS             complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGR46_000529"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0855533.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(27117..27797)
                     /locus_tag="WGR46_000530"
     CDS             complement(27117..27797)
                     /locus_tag="WGR46_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0855534.1"
                     /translation="MERYKNAVGKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDGNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQHNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGR46_000531"
     CDS             complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGR46_000531"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0855535.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGR46_000532"
     CDS             complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGR46_000532"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0855536.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDTSVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(29669..30169)
                     /locus_tag="WGR46_000533"
     CDS             complement(29669..30169)
                     /locus_tag="WGR46_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0855537.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLKDAKKAQAEEKWCGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGR46_000534"
     CDS             complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGR46_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0855538.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGR46_000535"
     CDS             complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGR46_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0855539.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(34060..35295)
                     /locus_tag="WGR46_000536"
     CDS             complement(34060..35295)
                     /locus_tag="WGR46_000536"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0855540.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(35300..36097)
                     /locus_tag="WGR46_000537"
     CDS             complement(35300..36097)
                     /locus_tag="WGR46_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0855541.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(36100..36846)
                     /locus_tag="WGR46_000538"
     CDS             complement(36100..36846)
                     /locus_tag="WGR46_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0855542.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGR46_000539"
     CDS             complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGR46_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0855543.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(37640..38767)
                     /locus_tag="WGR46_000540"
     CDS             complement(37640..38767)
                     /locus_tag="WGR46_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0855544.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(38837..38912)
                     /locus_tag="WGR46_000541"
     tRNA            complement(38837..38912)
                     /locus_tag="WGR46_000541"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38877..38879),aa:Thr,seq:cgt)
BASE COUNT         9363 a         8721 c        10584 g        10321 t
ORIGIN      
        1 ttgcgctttg cgattcgcga aggcggccgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaat tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag
      121 cttatgatta cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta
      181 ccggtgcagt tgtaaaaggc ccgattcctt tgccgaccaa aatcgaacgt ttcaacattc
      241 tgcgttctcc gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccacttgc
      301 gcctgatgga catcgtggat tggaccgaca aaactaccga tgcgctgatg aagctggatt
      361 tgccggccgg tgttgatgta gaaatcaaag tccaataatt agggctgttg aaaatcccca
      421 agcaattaat gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca
      481 gatttaaggc aaaacaacaa aatcaaaaaa cagtgcggtc ggcgaaatcg gcttattttg
      541 tgcctcaaga attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat
      601 agccttccga aggcttgccc ccccccgaag aaaggtagaa tcgggcttgg tttgggcaga
      661 agcatccatc tgattctaag gatggatgat gttttattct caagtaaata aaggaaagat
      721 gatgagctta cttaatgtct ttaaagatat tttaaacaac aaagaattga attcggaaga
      781 acggcatgat ttggagcggg cggttaagat cttgcaggat acgcatgtca atattttgat
      841 taccggagcc accggttgcg gaaaatcatc caccatcaat gccttgttca gcatagatca
      901 ggcaacggga aagagtgatg gactagtaaa ggaagtcgca aaagtaggcg tgggtgtaga
      961 tcctgaaact atggatattg ccaaatatga attggagaat atgacgattt gggatagtcc
     1021 gggcttgggc gacggagaga aggcagacca aaagcaccgg cgcaatatca tcaataagct
     1081 gctggagaaa gacagtaacg gcaatgccgt catcgacttg gttttggtgc tgcttgacgg
     1141 cggctccaga gacttgggga catcatacga actgattaac aatgtaatca tcaatacatt
     1201 gaagaagagt ggggaaggcc gtacagaccg gttgctggtt gcgattaatc agtgtgatat
     1261 ggcgatgaag gggcggaatt gggatgcgga agccggccgt ccgaacgccg ccttggaaaa
     1321 ctttttggag gagaaagtgt gttcgacacg caagcgcatt caggaagcta ccggcgttac
     1381 ggtcaatccg atttattttt cagcaggctt taagagtgag gatgaggagc agcgtcccta
     1441 taacataacc cgcctgttgc gttacatcat tacggctatg ccgtctgaaa aacgggtgat
     1501 tgtggcagag aagattaatg tggaagaaat cgaacgagat accagccgcc gcaaacagga
     1561 ggagcggcaa gaaatcgaca gtagtatcgg cagtgcgctg gagaaaatat tgggggctgc
     1621 gggaggcgtg gttaaagatg ttgtttcaac gatagcaagc ggtgtcggga cgctgatcgg
     1681 cggtgctgca aaagcgattg gaggggcgat tagcacggtt gcttcttttt ttggctggta
     1741 ggagcgcata tgcatcgtta ccgtatttct gaaatcgggc aaaaagtcac agcagccggt
     1801 ttccgtcatt tggatgtctt gcttgtcggt gcgacaggtg tcggcaagtc ctcaacctta
     1861 aatgccttgt ttggcgaaca aaaagccaag gtaggcacag gcgtggatcc cgaaacgcaa
     1921 ttggtcgaaa gctatatgct gaacgatgtg ttgcggtttt gggacagcgc aggtttgggc
     1981 gatgggaaag aagccgaccg cgcccatcgg caaaaactga ttgatgtcct gtctaaaacc
     2041 tatactcatt cggatgggca gtggggctgg atagatttgg tgttcgttat ccttgacggc
     2101 agctcccgcg atttgggtac ggcctatgat ttgttgaggg atgttatcct taaaatgatt
     2161 gatccggata gggttattgt tgcaatcaat caggcggata tggcgatgaa aggacgttat
     2221 tgggataagg tgctgcatca gccgcagccg aatttgcaac agtttctaga cgagaaggcc
     2281 gaatcggtac aaaaaaggat tctggaagca accggtttgc agatttccaa acccgtttac
     2341 tattcggcat acgaaaacta ccatctcaaa gaaataatgg atgcagtcat caaccatatc
     2401 ccggtttgtc ggagaaagat gcatacgccg tgatggtcgg ataccgactc ctttaaaagc
     2461 atccgaccct atgccgtctg aagattcaaa acgcttcaga cggcatattg aagatatttt
     2521 tgatattttt gttgatattt ctttgacttg tcagatataa tgtctgactt ggtacattcg
     2581 taccaagttt aactttgtct gaaagacagg ccaatcgtag cctgtccctt tactttaaaa
     2641 ggaaaataat catgacttta ggtctggttg gacgcaaagt tggtatgacc cgcgtgttcg
     2701 acgaacaggg tgtttctgtt ccggtaaccg ttttggatat gtctgccaac cgcgttacac
     2761 aagtaaaatc caaagatact gacggctata ctgccgttca agttaccttt ggtcagaaaa
     2821 aagccaatcg tgtcaacaaa gccgaagccg ggcactttgc aaaagcaggt gttgaagccg
     2881 gtcgcggttt gattgagttt gctttgactg aagaaaaact ggctgaattg aaagctggtg
     2941 acgaaatcac cgtttctatg tttgaagtcg gtcaactggt cgatgtaacc ggtacctcta
     3001 aaggtaaagg tttctccggc acgatcaaac gtcataactt cggtgcccaa cgtacttccc
     3061 acggtaactc ccgttctcac cgtgttccag gctctatcgg tatggcgcaa gacccgggtc
     3121 gcgtgttccc cggtaaacgc atggccggcc aatacggcaa caccaaagca actgttcaaa
     3181 aattggaagt tgtccgtgtt gatgcagaac gccaactgct gttggttaag ggtgctgttc
     3241 cgggtgcggt caacagcgat gttgtagttc gtcccagcgt gaaagtaggt gcgtaatgga
     3301 attgaaagta attgacgcta aaggacaagt ttcaggcagc ctgtctgttt ctgatgcttt
     3361 gttcgcccgc gaatacaatg aagcgttggt tcatcagctg gtaaatgcct acttggcaaa
     3421 cgcccgctct ggtaaccgtg ctcaaaaaac ccgtgccgaa gtaaaacact caaccaaaaa
     3481 accatggcgt caaaaaggta ccggccgcgc ccgttccggt atgacttcat ctccgctgtg
     3541 gcgtaaaggt ggtcgcgcgt tcccgaacaa acccgacgaa aacttcactc aaaaagtaaa
     3601 ccgcaaaatg taccgtgccg gtatggcgac tattctgtcc caattgactc gtgacgagcg
     3661 tttgtttgcg attgaggcgt tgactgctga aactcctaaa accaaagttt ttgctgaaca
     3721 agtgaaaaat ctgggtctgg agcaagtgtt gtttgtaacc aaacagctcg acgagaatgt
     3781 ttacttggct tcacgcaact tgccaaacgt gttggttttg gaagctcaac aagttgatcc
     3841 ttacagcttg ctgcgttaca aaaaagtaat catcactaaa gatgcagttg cacaattaga
     3901 ggagcaatgg gtatgaatca acaacgtttg actcaagtga ttttggcacc tatcgtttct
     3961 gaaaaaagca acgtattggc tgaaaaacgc aaccaaatga cgtttaaagt cttggcaaat
     4021 gcaaccaaac ctgaaatcaa agctgctgtt gagctgctgt ttggtgttca agttgcttct
     4081 gtaactaccg ttacaactaa aggcaaaact aagcgttttg gtcgtacttt gggccgccgc
     4141 agcgatgtta aaaaagctta tgtaagcttg gctgccggtc aagagttgga tttggaagcc
     4201 gctgctgcag ctgcagataa ggaataaaca aaatggcaat cgttaaaatg aagccgacct
     4261 ctgcaggccg tcgcggcatg gttcgcgtgg taacagaagg tttgcacaaa ggtgcacctt
     4321 atgcgccctt gcttgaaaag aaaaattcta ctgccggccg taacaataat ggtcatatca
     4381 ccacccgtca caaaggcggc ggtcataaac accattaccg tgttgtagac tttaaacgta
     4441 acaaagacgg cattcctgcc aaagtagagc gtatcgaata cgatcctaac cgtactgcct
     4501 tcatcgcact gttgtgctat gcagacggcg agcgtcgtta catcattgct cctcgcggta
     4561 ttcaagccgg tgctgtattg gtttccggtg ctgaagctgc catcaaagta ggcaacaccc
     4621 tgccgattcg caacattccc gttggtacga ctatccactg tatcgaaatg aaacccggta
     4681 aaggtgctca aatcgcacgt tctgccggtg cttctgcggt attgctggct aaagaaggcg
     4741 cgtacgctca agtccgtctg cgctctggcg aagtccgtaa aatcaacgta aattgccgtg
     4801 cgaccattgg tgaagttggt aacgaagagc aaagcctgaa aaaaatcggt aaagccggtg
     4861 ccaaccgttg gcgcggtatt cgtccgaccg tacgcggtgt tgtcatgaat cctgtcgatc
     4921 acccgcatgg tggtggtgaa ggccgtacgg gcgaggcccg cgaaccggtc agcccatggg
     4981 gtactcctgc taaaggctac cgcactcgta ataacaaacg cacggataac atgattgttc
     5041 gtcgccgtta ctcaaataaa ggttaattta gtatggctcg ttcattgaaa aaaggcccat
     5101 atgtagacct gcatttgctg aaaaaagtag atgctgctcg cgcaagcaac gacaaacgcc
     5161 cgattaaaac ctggtctcgt cgttctacca ttctgcctga ttttatcggt ctgaccattg
     5221 ctgtgcacaa cggccgcacc catgtgcctg tgtttatcag cgacaatatg gttggtcata
     5281 aattaggcga attctcattg acccgtacct ttaaaggcca cttggccgat aaaaaggcta
     5341 aaaagaaata aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc
     5401 tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa
     5461 tattttggct ttcagtccta aaaaaggtgc cgagctgatt aaaaaagtat tggagtcagc
     5521 tattgctaat gccgagcaca ataacggtgc ggacattgat gaactgaaag tggtaactat
     5581 ctttgttgac aaaggcccaa gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa
     5641 ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat
     5701 gggacaaaag attaacccta caggctttcg cctggcggta actaaagact gggcttcaaa
     5761 atggtttgct aaaagcaccg acttttctac tgttttgaag caggatatcg atgttcgcaa
     5821 ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga gtggttattg aacgccctgc
     5881 aaaatctgca cgcattacca ttcactccgc tcgtccgggt gtggttatag gtaaaaaagg
     5941 tgaggatatc gaggttttga aacgtgactt gcaagtcttg atgggtgtac ctgttcatgt
     6001 aaatattgaa gagattcgcc gtcctgagtt ggatgctcaa attattgctg acggtattgc
     6061 ccagcagttg gaaaagcgcg ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc
     6121 aatgcgttct ggtgctaaag gcattaagat tatgacttca ggccgtctga atggtgcgga
     6181 tattgcccgt agcgaatggt atcgtgaagg tcgcgtgcca ctgcatactt tacgtgcaaa
     6241 tgtagattat gcaaccagcg aagcgcacac cacatatggt gtattgggtc tgaaagtttg
     6301 ggtttatacg gaaggcaata ttaaatcttc caaacctgaa catgagagta aacaaagaaa
     6361 ggcaggtaga cgtaatgctg cagccaacta gactgaaata ccgtaagcaa caaaagggtc
     6421 gcaatactgg catcgctact cgcggtaata aggtaagttt cggtgagttc ggcttgaaag
     6481 ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc
     6541 gtcatatcaa acgtggtggt cgtatttgga ttcgtgtatt ccctgataaa ccgattactg
     6601 aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat tacattgccg
     6661 aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg
     6721 aagcattcga gttggctgct gccaaattgc ctattcctac aacctttgta gtaagacagg
     6781 tgggtcaata atgaaagcaa atgaattgaa agacaaatcc gttgagcagt tgaatgcaga
     6841 tttgttggac ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt
     6901 aggcaaacca agtgaattga aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt
     6961 aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa
     7021 gtagtaagcg acaaaatgga taaaaccgta acagtattgg ttgagcgtaa agtaaaacat
     7081 ccgctgtatg gtaagattat tcggttatct actaaaatcc atgcccatga tgaaaataat
     7141 caatatggaa ttggtgatgt ggttgttata tcggaatccc gtccattgtc aaaaactaaa
     7201 tcttgggttg tcagtgagct ggttgagaaa gcacgttcta tttaagaatt aaagcaacgt
     7261 gcttggaatg ggaaacgaag tattgcagca aatttaattt gcgtgtaaac ttcgtttcct
     7321 gtctttcagt ttcttctgga agtttcttcc ctttcggggt ccaagactgg tttacttgaa
     7381 ccgcaaggtt tcatttaata agcagcggct ttgctgtaag ttatctgaaa gtggtaaatt
     7441 aagttggtta atttaaaggt aataacatga ttcaaatgca gaccatctta gatgtggctg
     7501 ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt gggcggatct aagcgtcgct
     7561 acgcttctgt tggcgatatt attaaagtgg cagttaaaga tgcggctccg cgtggccgtg
     7621 tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac tgctaagggt gtacgtcgtc
     7681 ctgatggtgc gttaattaaa ttcgataaca atgccgccgt gttactgaat aataaacttg
     7741 aacctttggg tactcgtatc tttggtccgg taacccgtga attgcgtact gagcgattta
     7801 tgaaaatcgt ttcattggca cctgaagtat tataaggaat ggcacgatga ataaaatcat
     7861 taaaggcgat agggttgtag taattgctgg taaggataaa ggtaagcagg gtcaagtagt
     7921 tcgagtgttg ggtgataaag ttgttgttga gggcgttaat gttgtaaaac gccatcaaaa
     7981 acctaatcca atgcgtggca ttgagggcgg tattattact aaagaaatgc ctttggatat
     8041 ttctaatatc gcaatcctga atccggaaac taataaagcg gaccgtgttg gtattaagct
     8101 gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc aaatcaaatg gctctatcat
     8161 tggggcataa ggagataaca tggctcggtt gagagagttt tataaagaga cagttgttcc
     8221 tgaattggtt aaacaatttg gttacaaatc agtaatggaa gtcccgcgta ttgaaaaaat
     8281 caccttgaat atgggtgtgg gtgaggctat tgctgataaa aaagttatgg aacatgctgt
     8341 ttccgattta gagaaaattg ccggtcaaaa accggttgtt actgttgccc gtaaatctat
     8401 cgcaggtttt aaaatccgtg ataactatcc ggttggttgc aaagtaacat tgcgtcgtga
     8461 tcaaatgttt gaattcttgg atcgtttgat tactattgca ttacctcgcg tacgtgactt
     8521 ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat tacaatatgg gtgttcgtga
     8581 gcaaattatt tttccggaaa ttgaatacga taaaattgat gctttgcgtg gtttgaatat
     8641 tactattact actacagcaa aaaccgatga ggaagcgaaa gctttattgt cattgtttaa
     8701 atttccgttc aaaggataat catggctaag aaagcactta ttaatcgtga tctgaaacgt
     8761 caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa ttaaagcggt aatcaatgat
     8821 tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga ggtttcaatc cattcctcgt
     8881 aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga caggtcgccc tcgtggtact
     8941 ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa tcgccatgcg tggcgaaatt
     9001 ccgggtgttg ttaaagccag ctggtaatag gagtaattaa gaatgagtat gcatgatcct
     9061 atttccgata tgttgactcg tatccgcaat gcgcaacgtg ctaataaagc agcggttgca
     9121 atgccttctt caaaattaaa gtgtgctatt gcaaaggtat tgaaagaaga aggatatatt
     9181 gaggacttcg cagtttcatc tgacgtaaag tctatattgg aaattcaatt aaaatactat
     9241 gcaggtcgtc ctgtaattga acaaatcaag cgtgtatctc gccccggttt gcgtatttat
     9301 aaagcgtcta gtgagattcc aagtgttatg aatggcttgg gtattgctat tgttagtact
     9361 tctaaaggtg taatgactga tcgtaaagca cgttctcaag gtgttggtgg tgagttgtta
     9421 tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc gcaaaaaacc cagtgactgt
     9481 tcccgctggt gtagaagtaa aatttggaac agaggcatta gttattaagg gtaagaacgg
     9541 tgaattgtct tttcctttgc attctgatgt agccattgaa tttaatgatg gcaaattggc
     9601 ttttgttgcg aataacagca gtaaacaagc aaatgcaatg tctggtactg ctcgcgcatt
     9661 agtcagcaat atggttaaag gtgtttcaga aggttttgag aaaagattgc aattgatagg
     9721 tgtgggttat cgtgctcaag cacaaggtaa aatcttgaat ctgtctttgg gtttttctca
     9781 tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa actcctagcc aaacagagat
     9841 tgttttaacc ggctcggata aacaagttgt tggtcaagtt gctgctgaga ttcgtgcgtt
     9901 ccgtgctcct gagccttata aaggtaaagg tgttcgctat gtaggcgaag tagtggtaat
     9961 gaaagaagcc aagaaaaaat aattgaggtt cactaatgga taaacataca acccgactcc
    10021 gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa aatggtaaga ttatgtgtgt
    10081 tccgaagcaa taatcatatt tatgctcaag taattagtgc tgaaggtgat aaagtattgg
    10141 ctcaagcctc tacattggaa gctgaggtgc gcggtagtct gaaatctgga agcaatgttg
    10201 aagcagctgc aatagttggt aaacgtatcg ctgagaaggc taaagcagca ggtgtagaaa
    10261 aggttgcttt tgatcgttca ggtttccaat atcacggtcg tgtgaaggct ttggctgaag
    10321 ctgctcgtga aaatggttta agcttctaaa tatttggaga ctttcagatg gcaaaacatg
    10381 aaattgaaga acgcggtgac ggtctgattg aaaagatggt cgcagttaac cgtgtaacca
    10441 aagtagttaa aggtggtcgc attatggctt tctctgcgct aactgttgtt ggtgatggag
    10501 atggtcgtat tggtatgggt aaaggtaagt caaaagaagt gcctgttgca gtccaaaagg
    10561 cgatggatca agcacgacgc tctatgatta aggtaccatt aaaaaatggt acgattcatc
    10621 atgaggttat tggtcgacat ggtgctacta aagtatttat gcagcctgct aaagagggta
    10681 gtggcgtaaa agccggtgga cctatgcgtt tggtttttga tgctatgggc attcataata
    10741 tctccgccaa agtgcacgga tctactaacc catataatat cgtacgtgca acattagatg
    10801 gtttgtctaa gttgcatact cctgctgata tcgcagccaa acgtggcttg acagtggaag
    10861 acattttggg agttaaccat ggctgaacaa aaaaagatta gggttacatt ggttaaaagc
    10921 ctgattggta caattgaatc tcatcgtgca tgtgcacgcg gtttaggttt gcgtcgtcgc
    10981 gagcatacgg tagaggtttt agatacccct gaaaaccgtg gtatgattaa taaaatcagc
    11041 tacttgttga aagtggagtc ttgatatgtt tttgaataca attcaacctg ctgttggtgc
    11101 tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt ggtcttggca aaacgggtgg
    11161 tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt cataaggtgg gtttcgaagg
    11221 tggtcaaatg cccttgcaac gacgcctccc taaaagaggt tttaaatctt taacagcatc
    11281 agctaatgca cagcttcgtt taagtgaact ggaatcaatt gctgttaatg agattgatat
    11341 tttggtctta aagcaagcgg gtctgattgc atctacagtc tctaatgtta aagttattgc
    11401 ttttggtgaa atttctaagg cagttgcttt gaagggtatt aaagttacca aaggtgcgag
    11461 agctgctatc gaggctgttg gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat
    11521 caacaaacgt catcaggttc atccaaattt ggagatctta agaaacgtct tttgtttcta
    11581 tttggagcat tgattgtttt tcgaattggt gcccatatac ccgtacctgg agttgatgct
    11641 gttgctttag ctaaattata cgaaagcgct ggaaacggca tcctgggaat attgaatatg
    11701 ttttccggtg ggtcgttaga gcgctttagt atatttgcaa taggaattat gccatatatt
    11761 tcagcttcta ttattgtaca gctcgcttct gaaattttgc catcattgaa ggctttaaaa
    11821 aaagaagggg aggctggtag aaaggtaatt acgaaatata ctaggtatgg tactgttttg
    11881 ttagcaattc ttcaaagtct aggtgttgca tctttcgtat ttcagcaagg aattgttgta
    11941 acaagttcat ttgagtttca tgtttccacg gtagtttctt tggtaacggg aactatgttt
    12001 cttatgtggc ttggggagca aattactgaa aggggtatcg ggaacggtat ttctttaatc
    12061 attacggcag gtattgcttc aggtattcct tcgggtattg caaagctggt tacactgacg
    12121 aaccaaggtt ctatgagcat gcttacggcg ttgtttattg tatttggtgc cttattatta
    12181 atttatttgg ttgtatactt tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa
    12241 cgccagttta atggtagggc gggtagtcaa aatacgcata tgcctttcaa gttgaatatg
    12301 gctggtgtta ttcccccaat ttttgcttcc agtattattc tatttccatc tactctttta
    12361 ggttggtttg gttcggctga tacaaatagt gttttgcaca aaatagctgg attgttacaa
    12421 cacggtcaat tgctgtatat ggctttattt gcagcgacag ttattttctt ttgttatttt
    12481 tatacggctt tggtttttag ccctaaagaa atggcagaga atttaaaaaa gagtggtgct
    12541 tttgttcctg ggattagacc tggtgagcag acctctaggt atttagaaaa agttgtatta
    12601 cgtttgacat tgtttggagc tctttatatt acaactattt gtttaattcc agagttctta
    12661 actacggttt taaatgtacc tttttatttg ggtggcacgt ctttgttgat tctagttgtt
    12721 gtaacgatgg attttagtac acaaataaat tcgtataggc ttactcaaca gtatgataag
    12781 ttaatgactc gttcagaaat gaaatcattt tctcggaaat agaattatgg cgaaagaaga
    12841 tactatccaa atgcaaggtg aaattcttga aactttacct aatgcaacat ttaaagtaaa
    12901 acttgagaat gaccatattg tattgggtca tatttctggg aagatgcgga tgcattacat
    12961 tcgtatttct ccgggagata aggtcacagt agagctgaca ccttatgatc taactagggc
    13021 tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca
    13081 tctgttaaga aaatttgccg aaattgcaag attattcgtc gaaatcgtgt agttcgtgta
    13141 atttgtactg atctccgtca caaacagcgt caaggttaat ggaatatttc ttttaatgtg
    13201 attctgtgat atagtgacac actttgccct aaaaaggaaa aaatatggct cgtattgcag
    13261 gggtaaatat ccctaataac gcacacatcg taattggtct tcaggctatt tacggtattg
    13321 gtgctactcg tgctaaattg atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa
    13381 aagatttgga cgagactcaa ttagatgctt tgcgtgacca agttgccaag tatgaagtag
    13441 aaggtgattt gcgtcgtgag gtaactatga gtatcaagcg attgatggac atgggctgct
    13501 atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg tcaacgcact cgtacaaatg
    13561 cgcgtacccg caaaggtccg cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt
    13621 ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg
    13681 agtgagggta ttgtgcacgt tcatgcatct ttcaacaata ccatcattac aatcactgac
    13741 cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg ctggttttaa aggttctcgt
    13801 aaaagtacac catttgcagc acaagttgca gcagaagcag ctggtaaagt tgcccaagag
    13861 tatggcgtta aaaatttaga ggttcgtatt aaaggtccag gtccaggtcg cgaatcctct
    13921 gtacgtgctt tgaatgctct tggtttcaag attaccagca ttactgacgt taccccgttg
    13981 cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa
    14041 catggcacgt tatattggcc ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt
    14101 tttgaagagt gcgcgccgct ctttggattc taaatgtaaa attgattccg ctcctggtca
    14161 gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa
    14221 aatccgccgt atttatggcg tattagaacg tcagttccgt cgttatttcg cagaagctga
    14281 tcgtcgtaaa ggttctaccg gcgagttgct gttgcagttg ctggaatctc gtttggataa
    14341 tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa gcaagacagc ttgtttctca
    14401 taaggcgata gttgtgaatg gacaagttgt caatattcct tctttccaag tgaaagctgg
    14461 tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta cgtattcaag aagcattggg
    14521 tttggcaact caaatcggct tgccgggttg ggtttctgta gatgcggata aacttgaggg
    14581 tgtgttcaaa aacatgccgg atcgctcgga attgaccggt gatattaatg aacagctggt
    14641 ggtagagttc tactctaaat aatgctagct cagtgaggga cagttaaatg cagaatagca
    14701 caaccgaatt tttgaaacct cgtcaaattg atgtaaatac tttttctgca actcgtgcaa
    14761 aagtatctat gcagccattt gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc
    14821 gtatcttact gtcatccatg aatggttttg ctcctactga agtagctatt gccggtgtat
    14881 tacacgaata ttctactgtt gatggtattc aggaagatgt tgttgacatt ttgctgaata
    14941 ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat
    15001 caggttcagg tgtcgtatct gccggtgata ttgagttgcc gcatgatgta gaaattctga
    15061 atcctggtca tgtcatttgt catttggctg ataacggtca aattgagatg gaaattaaag
    15121 tagagcaagg tcgtggttat caatctgttt caggtcgtca ggtagttcgt gatgagaacc
    15181 gtcagattgg tgcaatccag ttggatgcga gcttttcgcc catcagccgt gttagctttg
    15241 aggttgaacc tgcacgtgta gagcagcgga cggatcttga taagttggtt ttggatatcg
    15301 aaaccgacgg ttctattgat cctgaggaag ctgtacgcag tgcggcacgt attttgattg
    15361 atcagatgtc tatttttgct gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag
    15421 cacctcctat cgaccctgtt cttttgcgtc cggtggatga tctggaattg acagtacgtt
    15481 cagctaattg tttgaaagct gaggatattt attatattgg cgatttgatt caacgcactg
    15541 aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc tttgaatgag attaaggaag
    15601 tattggcatc taaaggtttg acactgggtt ctaagttgga agcatggcca cctgtaggct
    15661 tggaaaagcc ttaatgaaga attaaaggat aattgatatg cgtcatcgta atggcaatcg
    15721 caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt
    15781 attgactcac gaaactattg taacaactct gcctaaggcc aaggaattgc gccgtgtagt
    15841 agagccgttg attacattgg gtaaaaagcc gtcattggca aaccgccgtt tggcatttga
    15901 ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc gatttgggtc ctcgttttac
    15961 tgctcgtaac ggtggttatg ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc
    16021 acctctggca ctggttgaat tggttgacaa accggctgct gagtaatttt agtcatataa
    16081 cgccatctgc cgaaaagcag gtggcgttat ttttgcaata tctgataggt aatagggtat
    16141 tggctatcat gtttaaaata ttaattgaat agctaaggtt tgcgcggtaa acttacatca
    16201 ttaaaaaatt ctatgatggt ttatataatg aatgctttcg atataaagtc gacaaagatg
    16261 gacgtattgt ctatatcttt gcatacgtca gacttgtttg atttggaaga tgtgctggtc
    16321 aaattgggca agaagtttca agagtctggt gttgttccat ttgtgctgga tgttcaagag
    16381 tttgattatc ccgagtcttt ggatcttgct gcattggttt cgttgttttc aaggcatggt
    16441 atgcaaattt tgggtctgaa gcattctaat gaacgttggg ctgctgcggc tatgaagtat
    16501 catttgctgt tttgtctgtc tcattcggaa aatgttaaag aactgggtca ggttgaggtg
    16561 cagaacacgg aggatggtca gaaagcaagg aaaacagtat tgattacatc ccctgtccgt
    16621 accggtcagc aggtttatgc cgaagatggc gatttgattg ttacgggggc ggtcagccag
    16681 ggggcggaat tgattgcgga tggcaatata catatttatg cgccgatgag ggggcgtgct
    16741 ttggccggtg ccaagggtga tacttctgcc cgcatattta tccactccat gcaggcagaa
    16801 ctggtttctg tggcgggtat ttaccgtaat tttgaacagg atttgccgaa ccatctgcac
    16861 aagcagccgg tacagatatt gttgcaggat aaccgattgg ttatcagtgc aattggctca
    16921 gagtaattgt ttgatatttt aaaaaggaaa tattgtggca aaaattattg tagtaacttc
    16981 aggtaagggc ggtgtcggta aaacgactac cagtgccagt attgcgacag gtttggcatt
    17041 acgcggatat aaaactgcgg taattgattt tgatgtgggt ttgcgtaacc tcgacctcat
    17101 tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat gtcattcagg gggaggcgac
    17161 gctcaaccag gctttgatta aagataaaaa ttgtgaaaac ctgtttattt tgccggcttc
    17221 ccagactcgg gataaagacg ctttgacacg cgagggcgta gaaaaagtga tgcaggagct
    17281 gtccggcaag aaaatgggct ttgagtatat tatttgcgac tctcctgccg gtattgagca
    17341 gggtgcattg atggcgttgt attttgctga tgaagccatt gtaacgacca atcctgaggt
    17401 ttccagtgtg cgtgactccg acaggatttt gggaattttg caaagcaaat cccataaggc
    17461 agagcaaggc ggttcggtta aagaacatct gttgattacg cgttattctc ccgaacgtgt
    17521 ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat attctgcata ttcctttgct
    17581 gggtgtgatt cctgaatccc aaaacgtctt gcaggcatcc aattccggag aaccggtcat
    17641 ccatcaggac agcgtggcgg cttccgaggc atataaggac gttattgccc gtcttttggg
    17701 cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa agcttcttca aacgtctgtt
    17761 tggaggataa ggtatgtcat taatcgaatt tttattcggc agaaagcaga aaacggcaac
    17821 cgttgcccgc gaccgccttc aaatcatcat tgcccaagag cgcgcccaag aaggtcaggc
    17881 tccggattac ctgccgactt tacgtaaaga gttgatggaa gtcctgtcca aatatgtgaa
    17941 tgtttcatta gacaatatcc gtatttccca ggaaaaacag gatggtatgg atgtgcttga
    18001 gttgaacatt actttgccgg aacagaaaaa ggtataggac atgaccttaa ccgaattgcg
    18061 gtacatcgtc gcagtcgccc aagaacgtca tttcggcagg gcggcgcggc gttgttttgt
    18121 cagtcagccc actttgtcta ttgccattaa gaaattggaa gaagagcttg ccgtctcttt
    18181 gtttgaccgg agcagcaacg atattattac gaccgaggcg ggggaacgta tcgttgcaca
    18241 ggcgcgtaag gtgttggaag aggcagagct tatcaggcat ttggcaaatg aagaacaaaa
    18301 cgagctggag ggtgcgttca aactcgggct gatttttacg gttgcgccgt acctgctgcc
    18361 gaaactgatt gtctcgttgc gccgtactgc accgaaaatg cctttgatgt tggaagagaa
    18421 ttacacgcat actttgaccg agtcgctcaa acgcggggac gttgatgcga ttatcgttgc
    18481 cgaaccgttt caagagccgg gcattgttac cgaacccttg tatgacgaac cgtttttcgt
    18541 gattgtcccg aaagggcatt catttgagga actggatgcc gtttcgcccc ggatgctggg
    18601 tgaggagcag gttttgctgc tgacggaagg caactgtatg cgggatcagg tactctcaag
    18661 ctgttccgaa ttggcggcga aacaacgtat acaggggttg accaatacat tgcagggcag
    18721 ctcgattaat acaatccgcc atatggttgc cagcggtttg gcaatcagcg tgttgccggc
    18781 aaccgcactg accgaaaacg atcatatgct gttcagcatt attccgtttg agggtacgcc
    18841 gccaagccgg cgggtcgtat tggcgtaccg ccgcaatttt gtccgtccga aggcgttgtc
    18901 ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg gtaagtttta tccgcgacta
    18961 ggcgcaggca ttgttttcaa aacgccattt ccctgagccg acaacacggt atgccaagat
    19021 attgccgtca tcatcgattt tgagtatagc atcgccacgg aaactgccgt cctgaagata
    19081 ttcgactttt gcatcactgt gaatgttttc atcagtgccg atgcaatgcc atgtatagtg
    19141 gattaacaaa aaccagtacg gcgttgcctc accttgccgt actatttgta ctgtctgcgg
    19201 cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa agaggccgtc tgaaaaacat
    19261 ttttcagacg gccttgttga ttcaatcaaa tcagtctttc aacttcgaca actgagtttg
    19321 cacttttgcc attttgtctt ccaattccgc caaatcggct ttgtcttttt ccaccaaatg
    19381 cgccggggct ttttcggtgt agccgggttt ggagagtttg gcgttgagtt tgtccaaggc
    19441 tttttgcagc ttctcggctt ctttgctcaa gcgggcggtt tcggcagctt tgtcgatttc
    19501 gactttcaac atcaggcgcg cgccgttgca gacggcgacg ggcgcgtctt cgctttcggg
    19561 tagggcggcg acttgctgtg cttcggtcag gcgggtcatc atcggcaggt atttgaggta
    19621 gtccgccaag tcgtctgcgc tttcgacaaa cagcggggct ttcacgttgg gctggatgcc
    19681 catttcgccg cgcaggttgc ggactgcgcc gatcaaatcc tgcaacacgg tcatttgccc
    19741 gaatgccgtc tgaacaatct cgccgctgtc ggcttcgggg aagcgggcga gcatgatgct
    19801 gtcggcggtt ttcgcgtcgc acataggagc gacggtttgc cacagttctt cggtgatgaa
    19861 cgggataatc gggtgcagca ggcgcagggc ggcttcgagt acgcgcaata aggtatggcg
    19921 tgtggcgcgt tggcggctgg cgcagccggt ttgaagctgc actttggcaa gttctaaata
    19981 ccagtcgcaa tagtcgttcc acatgaagct gtacagggtt tccgctgcca aatcaaagcg
    20041 gtaggtttcg taggcttgcg ttacttgctc gatggtctga ttcagacggt ctacaatcca
    20101 catatcgggg aaggaatagc cgcgcggctc agtggcggtt gcgccgtagc cgcagtcttg
    20161 gttttcggtg ttcatcaaga cgaagttggt ggcgttccag attttgttgc agaagttgcg
    20221 gtagccttcg gcgcgtttga agtcgaagtt cacgctgcgc cccaagctgg cgtagctcgc
    20281 catggtgaaa cgcaatgcgt ccgcgcccat actcggaatg ccttcgggaa agagtttttt
    20341 ggtggcttct tccactttcg gcgcggtttc aggtttgcgc aggccggtgg tacgttttac
    20401 cagcagcttg tccaagccga tgccgtcaat caaatccaca ggatcgatga cgttgccttc
    20461 ggatttggac atttttttgc cttcgtggtc gcgcacgatg ccgtggatgt acacgtcttt
    20521 aaacggtact ttgccagtaa agtgggtggt catcataatc atgcgcgcca cccagaagaa
    20581 gatgatttcg tagccggtta ccaagacgtt ggacggcagg aaggctttga gttcgtcggt
    20641 ttcagacggc cagccgagcg tggaaaacgg cacgagcgcg gaggagaacc aagtgtccaa
    20701 tacgtcttct tcgcgggtca agcctgtttt gccggcttgt ttttcggctt cagcctgatt
    20761 gcgggcgacg tacacattgc ctgtttcgtc gtaccacgcg gggatttgat gcccccacca
    20821 cagttggcgc gaaatacacc agtcttggat gttgttcatc cattggttgt aggtattgac
    20881 ccagttttca gggataaagc gtaccgcgcc gctgtcgacg gcttttttgg ctttgtcagc
    20941 aaggcttaag cctttgaatt cgctgtcagg ctcgccgccg tttggcgtgg cggacatggc
    21001 gacaaaccat tggctggtca gcattggctc aatcactgag ccggttcggt cgcctttggg
    21061 tgtcatcagc gtgtgtgctt tgatttcgac caacaagcct tgttcctgca aatcggcaac
    21121 catttgtttg cgcgcggcaa agcggtctaa acctgcgtat ttttcaggca gggcaaagcc
    21181 ttgttgcgct tcgcctttga agttgaacac ttcggcgttt gccagcactt tggcttccaa
    21241 gtcgaacaca ttaatcaggc gcgtgtcgtg gcgtttgccg acttcgtagt cgttgaagtc
    21301 gtgcgcgggc gtgattttca cgcagccggt gccgaagtct ttttcgacgt attcgtcggc
    21361 aatcacggga atggtgcggc cggtcagcgg caggattaat tccttgccaa tcaaatgggt
    21421 gtaacgttcg tcttcaggat tgacggctac ggcaacgtcg cccagcagcg tttcgggacg
    21481 ggtggtcgcc acgatgacgg cttcggaggg attgtccgcc agcggatagc ggatgtgcca
    21541 catagagcct tgttcttcca cgctttccac ttccaaatcc gataccgccg tgccaagaac
    21601 gggatcccag ttcaccaggc gtttgccgcg ataaatcaag ccctgttcat acaggcgtac
    21661 gaacacttct gtcacggttt cggcgcgcac gtcgtccatc gtgaaatact cgcgcgtcca
    21721 gtcggcagag cagcccacgc ggcgcatctg ctgcgtaatc gtaccgccgg aaacttcttt
    21781 ccattcccac actttttcca agaatttttc gcggcccaag tcatgacggg acacgttttg
    21841 cgcggcaagc tgacgctcga ccacgatttg cgtggcgatg cccgcgtggt cggtgccggg
    21901 aatccagacg gtgttgcagc ctttcatgcg gtagtaacgg gtcagaccgt ccataatggt
    21961 ttggttgaag gcatgaccca tgtgcagcgt gccggttaca ttgggcggcg gcagttggat
    22021 ggaaaaagac ggtttggtca aatccatatc aggttggaaa tagccttggc tttcccagtt
    22081 ttgatagtgc ttggattcga tttcggctgg attgtatttg tctaacatga tggaactttg
    22141 tgaaattaag gttatttttg atgtgcggat tataacgcaa aaaggccgtc tgaatcattt
    22201 cagacggcct ttggcataca ggttttaaaa atggaacaat accaggctga cggcaatcac
    22261 cgccatgccc attgtcaggc cgtaaacggt ttcatggccg tctgaatagc gtttggcagc
    22321 cggcagcagc tcgtccaacg ccaaaaacac catcacaccg gctatcacgc cgaataccga
    22381 accaaacacg gcaggcgaca aaaacggctg caaaaccaaa tagcccaaag ccgcccccaa
    22441 cggctcggcc aagccggata gcagacacgc ccacaccgtt ttcttacggc tgcgggtggc
    22501 aaaataaacc ggcgcggcga tggaaatgcc ctccggaata ttatggatgg caatcgccaa
    22561 ggccaaaggc atcccgactg ctggattttc caatgtggca aaaaacgtcg ccaagccttc
    22621 ggggaaattg tgcgcagtaa tcgcaaacgc cgccatcatg ccgactcgcg cgatatggcg
    22681 gcgtttgctt tcttgaaacg acgggtcttg cgcgtctaaa gtttcatgcg ggttcggcac
    22741 cagacggtca atcagcgcaa tgccgcccat ccctgccaaa aatgccatgg tcgccgccgc
    22801 aaacgcgtgg tctttatcat aaatttcagc gaacgcctcg ctggacttac tgaaaatctc
    22861 cgtcagggaa acatacacca tcgcaccgcc ggcaaatgcc aaaccaaacg acaacacgcg
    22921 cggattgggc gttttggaaa acatcaccaa gccgctgcct aatacggtaa acaaaccggc
    22981 agccaacgta atggaaaagg caacggccaa attggacatc gaaaaatcgg gcatgagaaa
    23041 acctgcgcta aaagctggga caggttcaga ctaacacttt ttaatgtata tgataatagt
    23101 tattatttat tttattgatt ggatacacgg attttgaaac aaaaggccgt ctgaaaaatg
    23161 attttcagac ggcctttaaa tttgaaatgc cgctaaacct tagtgctttc cagcttaagc
    23221 ctgataacgc gacaggctca aatcgtcgct gcggatttcg gtgtctttgc cgctcacgat
    23281 atcggcggtt aattttgccg aacccagcga catggtccag cctaaagtac cgtggccggt
    23341 attcagaaac aggttgtcaa agcgggtgcg accgattaac ggcgtgctgt cgggcgtcat
    23401 cggcctgagg ccgctccaga acgatgcttg gctcaaatcg ccgccttccg ggaacaagtc
    23461 gttgacgacc aaagccaagg tttcgcggcg tttttcgggc agtttgattt cgtagcccga
    23521 caattccgcc ataccgccga cgcggattct gttgtcaaag cgcgtgatgg cgactttgta
    23581 gctttcatct aaaacggtgg acaccggcgc gccgtcggaa tttgtgaccg gcagggtcaa
    23641 ggaatagcct ttgacgggat aaatgggcag attgagatcc aactgcgcca aaaccgtcct
    23701 gctgaaacaa ccgagcgcgc agacaacggc atctgcttca aaccgccctg tttcggtttc
    23761 aacggttttg atgcgcagcc cgttgtggtc gatgcggctg atggtttggt tgaaatgaaa
    23821 ccgtacgccc ttttcctgac acaatttgta taggttttca gtgaagaggc ggcagtcgcc
    23881 ggttgcatcc gcaggcaggt gcaggccgcc ggcaattttg gcggtaacgc gtgccagcgc
    23941 aggctcgaat tctgcgcatt cttcgggctt cagacggcgg tacggcacgc cgtagcgttc
    24001 caaaacggca atgtcttgtt ctgcagcttc aacttcttta gcctgacgga aaatctgcaa
    24061 cgtccctttt ttgcgccctt caaaattcat gccggtttgc gcttcaaaac ggcggaacat
    24121 ttcacggctg tattcggaaa tcctgaccat gcgctcttta ttggtttgat agtgcgctgc
    24181 cgtgcagttt tgcagcatcc gccacagcca ttcgatttga tacaggctgc cgtcggggcg
    24241 aaacagcaaa ggcggatggc ttttaaacag ccatttcagt gctttggtcg gaataccggg
    24301 tgcagcccaa ggcgtggtat agccgtaaga aagctggcct gcattggcaa aactggtttc
    24361 catcgccaca cccttggcgc ggtcgatgac cgttacttca tgtccggcct ctgccagata
    24421 ccacgcggaa gacacgccgg caacacccgc acctaaaaca agcactttca tgtttctccc
    24481 tccgactttt tcaaaacaga cttaatatgc cgtgccgtct gaatattcgg attcagacgg
    24541 cctcggatat taatgcggca attcgccgtt tgtgattttt tgtttgaagt cgcgcgtttc
    24601 attgacgatg actttcgcca tcaataaaag tgcaatcagg ttgggcaatg ccatcaagcc
    24661 gttgaatgtg tccgaagcca gccacaccaa atcaaggctc aacacggtac ccagcataac
    24721 ggaagaaaca taacccacgc ggtacaaacc ggcaaatttc tcgccgaaaa catacaccgc
    24781 gcatttttcg ccgtaatagc accagcccaa aatggttgag taggcaaaga aaatcaggcc
    24841 gatggtaaca atccagccgc cgatgccggg cagcattttt tggaatgtga cggttgtcag
    24901 tgccgcgccg ctcacttcag gtttgacaaa ctcgccgccc gcgccgagca gtcccattac
    24961 caacacgatg ccggtaatcg agcaaacgac gatggtatcc aaaaacgtac cggtcataga
    25021 aaccaaggcc tgacggacgg gatggtcggt tttcgcggct gcggcggcaa taggcgcaga
    25081 acccataccc gcctcattgg agaacacgcc gcgcgccacg ccgtagcgga tgaccgtacc
    25141 gatagcgccg cccgccactg cctgcgcgct gaacgcatcg gagaaaatca gcttgacggc
    25201 aggcatcagt gcatcggaat taatcgcgat aatggaaaga ccgcccaaca cataaaacac
    25261 cgccatagca ggcacgatga aagaagcggc tttggcgatg cctttaatac cacctaaaac
    25321 gacaacggca gtcagaacgg tcaacgtaat gccggtatag gcaggttcga taccgaagct
    25381 ggtttgcacc gcctgtgcaa ccgagttgga ctgcaccgag ctgccgatac cgaaggaagc
    25441 gaatgtgccg aacagcgcaa atgcaacggc catccatttc cagtttttgc ccaagccttt
    25501 ttcgatgtaa tacatcgggc cgccggacat ttcgcctttg gaattgttga cgcggtattt
    25561 caccgccaac acgccttcgc cgtatttggt ggccatgccg aaaatggcgg tcatccacat
    25621 ccaaaatacc gcgcccgggc cgccggttac caccgcagtc gccacgccgg cgatgttacc
    25681 cgtaccgatg gtggcggaca gcgcggtcat caacgccgca aaatgggaaa tatcgccttc
    25741 gtggccttcg ccgccttcat acttctttga cggcacaaac gcctgtttca gcgcatagcc
    25801 caacatagtg aactgtaagc ctttcagcat aacggtcagc acaatgccgg tgccgaccag
    25861 cagcatcaac ataacagatc cccagaccca gccgctgacg gtttcgaaaa aggctttgag
    25921 attgtctaaa aacaattgca tggctttctc ctttgtctgt tttattttta aaacaccact
    25981 tttgtagtgt cctgtaattt cagcacagaa tattcaataa gacaatatgt tcttttgaaa
    26041 aatacttttg gttttttctc cgaaagcagg tcggttcaag ctgcggaaat tgtttgcaat
    26101 tctttaaaag cagcggcgga ggtcacaatg aaatgtccga atggggatgt ggcgggcggc
    26161 agaaatcatc aatgctgccg actgccatac ttctgaaatc tacaaaatga tgcatcgatc
    26221 aaacaatata ccgctttaaa aaaaccgatg ccgtctgaaa cgctttcggg gtttcagacg
    26281 gcatcaaaag ggtacggtca gcggatgatg ccgcgcgccg attgtgcgaa aaagtctcgg
    26341 aatacggcaa gctcggcttg ggtttcggcg cggcggagga tgtccgcctt ggcctcttca
    26401 aacggaatgc cgcgatggta gagggttttg tacacgtctt tgacggcgga aatctgctct
    26461 gcggtaaaac cgttgcggcg cataccttcg ctgttgaggc ccgccggttc ggcgcggtag
    26521 cccgatgcca taaagtaggg cggtacgtct ttgtgtacgc ctgcggcgaa ggcggtcatg
    26581 gcgtagtcgc cgatgcggca gaattggaaa accagcgtgt agccgcccaa aacgacgtag
    26641 tcgccgatgg taacgtgtcc ggcaagcgag gcgttgttgg cgaagatggt gtggttgccg
    26701 accacgcagt cgtgcgcgag gtggcagtac gccataatcc agttgtcgtc gccgatacgg
    26761 gtttcgccga tgccggttac cgtgcctaaa ttaaaggtgg tgaattcgcg gatggtgttg
    26821 ccgttgccga taatcagctt ggtcggctcg tcgcggtatt ttttgtcctg cgggatttcg
    26881 ccgaggctgg caaattggaa aatgcggttg ttttcgccga tgctggtgtg gccgttgatg
    26941 acggcgtgcg gaccgatttc ggtgttcgcg ccgatttgga cgttggggcc gataacagtg
    27001 tacgcgccga ctttgacgct tgagtcgagt tcggctttgg ggtcgatgac ggcggtcggg
    27061 tggatgaggg tcatgttttt aatactccgc tgcttgttaa gacaaataat agaaaatcac
    27121 agtttgtcga tttcatcgca gaccgcaatc acttgatcgc taaaatggct aaatactgaa
    27181 gcagtatcta catccatgta attaagcagt tcaaaaatag attggtggat atttctagcg
    27241 tagccggtat cttttattgc acctttaaaa caaatcggct cgtgatgtgc caaacgatta
    27301 cggaaattat taatattgga aagttctcga aaaatccatt tttggttatg ttgcacactt
    27361 ggtgtagatt tgggcttttt tggaaaaact ttcatcaata ctttccctgt agcatcaaat
    27421 tgggcatctt tccctcccgc aaacagatac cgccaaaaac cgaatcctag tcctgcgacc
    27481 aattggttgt ggtcaaactt tcctccgcta ttattttgta ttttagataa tgcaaccttg
    27541 attaattcag cagattcttt cgtaccattt cttaaaaagc aaccttgata tttcaatgca
    27601 ggattcgttt gtggctgtat gctgttatat aaccagtttc cgtctttaaa cgcctgctga
    27661 aggcaaatgt caattttgtt tcttaaaaca atctcaaata ttgaaacaac atgaaaaagt
    27721 tctcttgata aagataaatt caataaatac aattcagctg ctttgtcttt cccaacggca
    27781 tttttatacc gttccattct gaaatcagaa aaaatactac agcattcctt ataattcacg
    27841 ctaatacctg attccttatt gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    27901 cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
    27961 cactataaaa ttgacgtttc aaactatatt tgatacagtt aggtgaaact aatccccacc
    28021 gcgtccctgg gtttgaataa acctaaaccg gtgggttttc tttttattag gtcgtctgaa
    28081 actttcagac gacctttttc cgaacgctca aaccacgcgt ttggcgcaca taatcacggc
    28141 ttcgacggcg acttgcccgt ccactttggc aacggcgttg aatttgccga tgccgcgccg
    28201 gctggtcagc agctcgactt caaagacgag ttggtcgccg gggatgactt ggcgtttgaa
    28261 acgggcttcg tctatgccgg cgaagaagaa gaattcgttt tccttgcgcc cgccttcgct
    28321 gaggatggcg agtgtgccgc aggcttgcgc catggcttcg atgatgagta cgccgggcat
    28381 cacgggcagg tcgggaaagt ggccttggaa ctggggttcg tttatggtta cgtttttgat
    28441 ggcggttagg gttttcatcg gctcgaaggc ggtgatgcgg tcgagctgga gaaacgggta
    28501 gcggtggggg atgagttttt ggatgtcttt ggcttcgatg gggagttgaa cgtccatgtc
    28561 tgtcgtattc cttgaataaa gtcggtttgg atttattgcg tgtctttgcc gtctgaaagc
    28621 tgctgttcca gcgttttgag gcgtttgttc atttcgctta agcggtggat gtaaacggcg
    28681 ttgcgcgccc attctttatg ggtggacatc gggaagatgc cggcgaggtg tttgccgctt
    28741 tcggtaatgc tgtgggtgac ggacgtgccg ccgccgatgg tggttttgtc ggcgatttcg
    28801 atgtgtccga ccgtgccgac gccgccgccg atgatgcagt agctgcctat ggtgacgctg
    28861 cctgagatgc cggttttggc ggcgatgacg gtgtgcgagc cgattttgca gttgtgtccg
    28921 atttggactt ggttgtcgat tttggtgccg ttgccgacgg tggtgtcgct catcgcgccc
    28981 cggtcgatgt tggtgttcga gccgatttcc acgtcgtcgc ccagcgttac cgcgccagtt
    29041 tgcgggattt taaaccacga atcgccggcg aaggcgagtc cgaatccgtc cgcgccgatg
    29101 accgcgccgc tgtggatttc aacgcgtctg cccagtgtgc agccgtaata aacgacggcg
    29161 ttaggatgta aaacaacttc atcacctaaa cggcaatcgt gttggacgac ggcgtttgcc
    29221 aagatgcggc agccttcgcc gagcacggtg tttgcgccga tgtaggcgtt cgcgccgatt
    29281 tcgcagcttg cgggaacggt cgcgcccggt tcgacgacgg cggtcggatg gatgccgccg
    29341 cgcgctttga cgacgggtga aaataggcgc gcgactttgg cgaaatagag ataggggtcg
    29401 tcggcgacaa tcaggttgcg cccttcaaat ccgtctgccg ctttggcgga aacgatgacc
    29461 gcacccgcgc tgctgtcgtg gacttcggct ttgtatttcg ggttggcgag gaagctgatg
    29521 tgttccgcct gcgcgtctgc gagcgggcgc acggcggtaa cagaagtgtc ttcgccgcgc
    29581 cattcgccgc cgagccgcgc ggtgatttgg gacagggtgt aggtggccgg aatcatggtt
    29641 ttcctgttcg gtatgccgtc tgaaagggtc agcgggcgtt catttcttta atgacgctgt
    29701 cggtaacgtc gtattgggtg ttgacgtaaa tcacgtcctg caaaatgaca tcgtaacctt
    29761 cctgtttggc gattttgacg atgacgcggt tggcgttttg ctggagggag gcaaactctt
    29821 cgttgcggcg gaggttgtag tcttcttcaa actgcgcctg ttttttgcgg aacgctgcga
    29881 ccagcccgca ccatttttct tcggcttgcg ccttttttgc gtctttgagt ttgccttcgg
    29941 caagctgcct ttccaaatcc agaccttcgc gttgcagttt ttgcaattcg tcctggcggg
    30001 cggaaaattc gctgtccagc gttttttgaa tcttgcgcgc ctgcttggat tcgaggtaga
    30061 tgcgctcggt gttgataaag ccgatttttt ggaaggtgtc ggcgtgcgcg cctgcggtgc
    30121 agcacaaacc gatcagagcc gcggcaaacg cgcgggtcaa acgggtcatg gtaaaactcc
    30181 ttcgaatgtt gccgcgaaat gccgtctgaa gggcttcaga cggcatttgc gggattagaa
    30241 cgtcgtgccg agttggaatt ggaagcgttg gatttcgtct tccggttttt tcttcagcgg
    30301 gtaggcgtag ctgaatttca tcgggcctaa aggcgagagc caggtaaccg cgccgccggc
    30361 ggaatagcgc aattcgttgg taaaggtgga tttatgggta ttgccggcgc cgtaaatgtt
    30421 ttgaaccctg ccgccggtcg cggaactgct gttgtcgtcg taggttttgc cgtcccacac
    30481 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgc gcgtctttcg cgccgggcat
    30541 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    30601 gccgtattcg tcatacactt tcggaccgag cgtgccgctt tcgtatccgc gcaccgaacc
    30661 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    30721 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    30781 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    30841 gttcacgccc gtcaggtagc cgcgcgtcgg ccataacgcg ctgtcggttt tgttgcgccc
    30901 ccagccgacg gtacctttgt acagccagcc tttgaagctg ccgtctgtgc cgtcggtttt
    30961 gccgtatttc ttgataaagt cggcatagtg tttgggcgct ttgttgtagg tgttgacggt
    31021 caggtgttct gccaccaaac cgaaattcac gcggtcgtat tcggtaacag gcacgctcat
    31081 gcggatgcct gcgcctgccg tggtggtttt atattgtttg atgctggtcg atgctttgcg
    31141 cgggtcgaag gcttttccgt aaacatcgta gcccaggctg accccgtctg ccgtgaagta
    31201 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgtgagg cgcgcagggc
    31261 ggccgacttg cccgtaccga acaggttgtc ttgggaaacg cctgcggaca tgaccaggcc
    31321 ggtatcctgt acccagcccg cgctcaagtc gagcgagccg gtggaacgtt cggtcaggct
    31381 catgttcaaa tcgactttgt cgggtgtgcc ggcaagcggg acggcatcaa actgtacgtt
    31441 gtcgaagtag cccaaaagct cgacgcgctc tttggagcgt tgcagcttgg aggtgtcgta
    31501 aggcgcggat tccatttggc gcaattcgcg gcgcacgact tcgtcgcggg ttttgttgtt
    31561 gccggtgatg tggatttcgt tgacgtagat tttccggccc ggttcgatgt gcaggacgaa
    31621 atcgacggtt ttggtttcgg cgtttggcag cggctgtacg ctgatttcgc tgtatgcgta
    31681 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    31741 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggctttgg ggacttcgtt
    31801 ggtgtcgcct tcgatggaga ctttgcccca acggaaacgt ccgccttcgt ggacggtgat
    31861 tttgatggtc tgcttggttt tgtcttcgtt ggtttggatg tcggtatcga ggatgcggaa
    31921 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcaaattt
    31981 ctgctcgttg aattggttgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    32041 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    32101 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    32161 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    32221 caggccggcg actgcctgat tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    32281 gaacgattcg aggtttttct taatggcgtc gttttgcagc atttttgcgc cggtgatgtt
    32341 gagcgagccg atggtggggc gttcgatgac ggtcagcagg agctgcccgt ccgcagtctc
    32401 gacgcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    32461 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tactcggctc
    32521 ggtacgctgc aagccttcga cgcggatgtc ttggatggtg aagtcggcaa gtgccaaagg
    32581 cgatatgccc aagaccatca gtgcggaagc aatctgtttc agtttcattg tcagttcctt
    32641 gtggtgcgga atgcggtttc agacggcatt ccgaaacgta aaatctaacc gagcagccgg
    32701 gtaacgtcgt tgaagaaggc gaccgccatc atcagcatca tgagggcaag cccgaagcgc
    32761 aaaccgatgt tttggacgcg ttcgcccaaa ggtttgccgc gtatccattc ggcagtataa
    32821 aacacgaggt ggccgccgtc caaaacgggg acgggcagta ggttcagcac gccgaggctg
    32881 atgctgacca gtgccaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    32941 gcaatgtcgg caatggtcag cggcccggaa atatggctga cggaggcgtt gccgctgatt
    33001 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    33061 ccgaatgcgc ggataacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    33121 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcggactg ttcgacagta
    33181 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtagttcag ggtgattttt
    33241 ttgccggggc tttggcgggt caggtttgcc cattcttgcc atgaggcgat gggtttgccg
    33301 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    33361 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    33421 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    33481 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    33541 gtttgcgcgc tgccccaatc tgcaacgggt gtgccgttga cggattgtat tttgtcgccg
    33601 ctttggaagc cggcgcgggc ggcaatggtg tcaggttcga ctgtgccgac gtaggggcgc
    33661 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    33721 gtcagtgggc cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    33781 tagggtaaat cggcttctga tacttcgcct tcgcgcgtgt cgaccatttt gacgtaaccg
    33841 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    33901 ttgccgaagc cgacggaaaa gcgtacgact ttgacaccgc acaatctggc aacgatgtag
    33961 tgtccgaact cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtg
    34021 tgcaaatggt tttcctttga taacggtgtt cggacggcat cagcgcagtg tgccgataaa
    34081 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaag agccccccta tgtcacctat
    34141 gccgtctgaa aagtcttgtg aaagacaatg ggcgacggtt ttggcaatgt cggtaaactt
    34201 aatctgtccg tccaaaaagg cggcgacggc ggcttcgttg gcggcgttca atacgcaggg
    34261 cgcgaccccg cccgcgttca tggcttcata ggcgagcttc aggcagggga agcggtcaaa
    34321 gtcgggcttt tggaaggtca gcgcggacaa tgcgtcgaaa tcaaggtcgc cgacacccga
    34381 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    34441 cagttgtgcc agcacggagc cgtcgcggta gcgcaccatg ctgtgtatca cggattgcgg
    34501 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    34561 ctccaaacct ttgttcatca tggtggcgga atcgacggag attttgcgcc ccatacgcca
    34621 attggggtgt ttgaccgctt gggcgggcgt aatgcggtcg aacgtgccta aatcggtcgt
    34681 taaaaacggg ccgccggaag cggtcaggat aatcgaacgg atgccgtgtt cgttcagacg
    34741 gccggcgtaa tcgcgcggca aaacttggaa aacggcgttg tgttcgctgt cgacgggcag
    34801 cactgccgcg ccgtttgcac gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    34861 ctctttgttc gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    34921 ccccaccgcc ccgacgatgg cgcacatgac accgctgact tcgtcggcag aggcaacgtc
    34981 aaccaatgcc tgcgcgccgt gtaaaacctg cgtcgccgtg ccgtcgcgtt tcaacagggc
    35041 ttcaagccgg gcggcgtatt cggcatcggc aacgacggca tattcgggtt ggaacgtttg
    35101 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    35161 ttcggggtgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    35221 tatggtcagg acttgtggtg tcataatggg gataacttta taccggatgc cgtctgaagc
    35281 gttttcagac ggcatagaat caatttaaaa ccgacatcat cgccgcatag acgctgataa
    35341 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc agcttgctgc
    35401 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa aaggtcgccg catacgctga
    35461 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    35521 agccggcact tcgtacggcg gtcatgtaca cggccacgca aaccgcgccg ccgattgcgc
    35581 cttcccagct tttgccgggg ctgattgccg gtgcgatttt gtgtttgccg aacgccttgc
    35641 cgctgaaata cgcgcaaata tcggcaaccc acaccaaacc catcacggcg agcagcggca
    35701 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgaccagaa
    35761 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccata
    35821 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    35881 agccgcccgc ataggcaacc acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    35941 taattttgca caaaccgccc atacgggcat attcccacaa ggcaatcagg gcaatcagtc
    36001 cgcaaaatgc agcccacaac cattgcggcg cgtaaaacag catgcccagc atcagcggca
    36061 gcagccacat ggcggtcatt acccgttgtt tcagcatatt cagttccttt gctgttcgat
    36121 aggcagttgc tcggaggtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    36181 atcgtccaaa gccttgccgt caaaatcggg ccacaaaata tcggtgaaat acagttcggc
    36241 ataagccatc tgccagagca ggaaattact gatgcgcgtt tcgccgccgg tgcggatgaa
    36301 caaatccggc tcgggcgcgt ctcccagcat caggtatttt gccagcgtgt cttccgtaat
    36361 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc aacgtccgcc
    36421 gtaatcggcg gcaatgctca gggtcaggcc ggtattgttt gccgtcaagg cttccgcctc
    36481 ttcgatgcct tgcagaatct gccgattaaa gcgttcgcgg ctgcccagta ttttcaggcg
    36541 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    36601 caggaacgaa acttcgtctt cggggcggcg ccagttttcg gtcgaaaagg caaatacggt
    36661 cagatattgc acgcccaatt tggcgcaatg cctcaccata ttttccaatg cgtccaaacc
    36721 gcgtttgtgt cccattatgc gcgggaggaa acgttttttc gcccaacggc cgttgccgtc
    36781 cataatcacg gcgatatgct tgggaatggc ggtgtgttcc aaaacggcct gcgtgctgct
    36841 tttcatgtct gcctttcgcg gttcggcatt caaatgccgt ctgaacgccg aaccgtgcag
    36901 gttaaattgc catcaaatct tcttctttgg cagtcaggag tttgtcggct tcggtaatgt
    36961 atttgtcggt cagtttttga accgcttctt cgccgcgacg tgcctcgtct tcggaaattt
    37021 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatgg
    37081 agacgcggcc ttcttccgct tcgccgcgta cgactttaat caggtctttg cggcgttcct
    37141 cggtcagcat gggcatcggc acgcggatca ggtcgccgac agctgccggg ttcagtccca
    37201 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    37261 cgccgatggt gcgcgcatcc agaagcgtta cgttggcaac ttggctgacg gggaccatgc
    37321 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    37381 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    37441 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaagcggg cagccgtctg
    37501 aaacgcgctt caaacctttc agacggcatg aaaactgtta actgcgaata gtaccgttat
    37561 tcgggcatga cgacaaggca ggcggattgg ggatgccgtc tgaagcggca ggcgtttcag
    37621 acggcatcgt gtccgaccgt cagccgtgtt cccgtgtttc aagcaggctt tggcgcaggt
    37681 gttggcgttc gtgggcatcc agccatttgc ggcgggtgcg ttgcagcagg atgacgaggg
    37741 cggaaatttc ctgacgcata ttggtgctga gccagaggaa gccctgccat tggtagtgga
    37801 ggtgttcggc gagggcttcc agttcggggt tgatggcggt gtcgatgcgg atgcggcggg
    37861 cgtgtctgcc gttgataagg gcgacggttt gttgcaggtc ggtttggagc agtgtgaagt
    37921 ggcggtcaag cagccggatt tcgctgccgt tgagtttggg agattgcagc ttggcggcag
    37981 tggtcaggag cagctcggtg gtgttgacaa ttttacggtg ggcgtgctgc atggcttcca
    38041 tcatggcggg gctgatgcgg ctttcgcccg atgtggcggc gaggtggctg cggcttttga
    38101 ccatgcgtgc gttgatttgg cgcattttaa ccatattctg ctccaaacgt tcgcgcgtca
    38161 tacgcctgcc gttgctgatt tcggcaatca ttttgctgca gtcggtcagg ttgtcggcaa
    38221 gcatgaaacg ccacatcagt gtggatttca gcggtagcag tttggcggcg gcgatggcga
    38281 tggccgcgcc gatgaggacg ttcatcgcgc gcatcaggcc gctgtcgaac cattcgctgc
    38341 cgttgtcgcc gatgagcatg cacatcgtca gccccgccag catagggacg tagccgtttt
    38401 tgccgaccgc cgcccagccg gccagtgcgc ttgccgtgcc gacggtgagg tagaagagga
    38461 ggttgccgtg gaaataatgc tggttcagcc ataaaacgcc caaacccgcg cccagcccga
    38521 tgaccgtgcc gagcatacgt tccaccgcct tggagtaaat cgcgccttga aactggagca
    38581 tgccgaggac gacgaagacg gtcatcccta tccactcgcc gtgttggagg tggagcagcc
    38641 gggcggaggc ggtggcgaac agaacggccc cgccgagccg gacggcgtgg atgaggcggc
    38701 ggtagcggta gcgttcgtag gagttgagcc agcggctgac gaggcggttg cgttgcgagg
    38761 tgttcatatc ggttgccgtc tgaagcggaa atgtgaaaaa gcacaggctt cccgagggag
    38821 ggtctgtgct tggtattggt gccggagaag ggaatcgaac ccccgacctt cgcgttacga
    38881 atgcgctgct ctaccgactg agctacaccg gcgtttttcg ttatgatata tatgaacggt
    38941 tgtttgtgca acttttctgg cgggcggcaa ggcagtgcgc ggtatagtg
//
LOCUS       DAWFKN010000011        33302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFKN010000011 DAWFKN010000000
VERSION     DAWFKN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.011"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WGR46_000542"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WGR46_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0855545.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGR46_000543"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGR46_000543"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0855546.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WGR46_000544"
     CDS             1963..2286
                     /locus_tag="WGR46_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0855547.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            2333..2809
                     /locus_tag="WGR46_000545"
     CDS             2333..2809
                     /locus_tag="WGR46_000545"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0855548.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="WGR46_000546"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="WGR46_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0855549.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WGR46_000547"
     CDS             complement(3678..4712)
                     /gene="pyrC"
                     /locus_tag="WGR46_000547"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0855550.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHISTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4772..5377)
                     /locus_tag="WGR46_000548"
     CDS             complement(4772..5377)
                     /locus_tag="WGR46_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855551.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5378..5602)
                     /locus_tag="WGR46_000549"
     CDS             complement(5378..5602)
                     /locus_tag="WGR46_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0855552.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5711..6322
                     /locus_tag="WGR46_000550"
     CDS             5711..6322
                     /locus_tag="WGR46_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0855553.1"
                     /translation="MRRAILLILALTAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            complement(6382..7254)
                     /locus_tag="WGR46_000551"
     CDS             complement(6382..7254)
                     /locus_tag="WGR46_000551"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0855554.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7291..8076)
                     /locus_tag="WGR46_000552"
     CDS             complement(7291..8076)
                     /locus_tag="WGR46_000552"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0855555.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESV
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            complement(8503..8595)
                     /locus_tag="WGR46_000553"
     tRNA            complement(8503..8595)
                     /locus_tag="WGR46_000553"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8559..8561),aa:Ser,seq:gct)
     gene            complement(8752..8949)
                     /locus_tag="WGR46_000554"
     CDS             complement(8752..8949)
                     /locus_tag="WGR46_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855556.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            complement(9054..9371)
                     /locus_tag="WGR46_000555"
                     /pseudo
     CDS             complement(9054..9371)
                     /locus_tag="WGR46_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9394..10155)
                     /gene="kdsB"
                     /locus_tag="WGR46_000556"
     CDS             complement(9394..10155)
                     /gene="kdsB"
                     /locus_tag="WGR46_000556"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0855557.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            complement(10152..10334)
                     /locus_tag="WGR46_000557"
     CDS             complement(10152..10334)
                     /locus_tag="WGR46_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0855558.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            complement(10403..10984)
                     /locus_tag="WGR46_000558"
     CDS             complement(10403..10984)
                     /locus_tag="WGR46_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0855559.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(11190..12224)
                     /locus_tag="WGR46_000559"
     CDS             complement(11190..12224)
                     /locus_tag="WGR46_000559"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0855560.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNEKSRAEDAGDEPLLLFRKTVAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(12264..12535)
                     /locus_tag="WGR46_000560"
                     /pseudo
     CDS             complement(12264..12535)
                     /locus_tag="WGR46_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12771..14051)
                     /locus_tag="WGR46_000561"
     CDS             complement(12771..14051)
                     /locus_tag="WGR46_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0855561.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(14273..14893)
                     /locus_tag="WGR46_000562"
     CDS             complement(14273..14893)
                     /locus_tag="WGR46_000562"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0855562.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            complement(14953..15948)
                     /gene="mltG"
                     /locus_tag="WGR46_000563"
     CDS             complement(14953..15948)
                     /gene="mltG"
                     /locus_tag="WGR46_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0855563.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            16032..16604
                     /gene="ampD"
                     /locus_tag="WGR46_000564"
     CDS             16032..16604
                     /gene="ampD"
                     /locus_tag="WGR46_000564"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0855564.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPNGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDDMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            16798..18084
                     /locus_tag="WGR46_000565"
     CDS             16798..18084
                     /locus_tag="WGR46_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0855565.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            18232..20757
                     /gene="ligA"
                     /locus_tag="WGR46_000566"
     CDS             18232..20757
                     /gene="ligA"
                     /locus_tag="WGR46_000566"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0855566.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKEAADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            20814..21989
                     /locus_tag="WGR46_000567"
     CDS             20814..21989
                     /locus_tag="WGR46_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0855567.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     ATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDTVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCFL"
     gene            22209..22469
                     /locus_tag="WGR46_000568"
     CDS             22209..22469
                     /locus_tag="WGR46_000568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855568.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            22576..23100
                     /gene="nspA"
                     /locus_tag="WGR46_000569"
     CDS             22576..23100
                     /gene="nspA"
                     /locus_tag="WGR46_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0855569.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            23236..23625
                     /locus_tag="WGR46_000570"
     CDS             23236..23625
                     /locus_tag="WGR46_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0855570.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            23703..24533
                     /locus_tag="WGR46_000571"
     CDS             23703..24533
                     /locus_tag="WGR46_000571"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0855571.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            24548..26005
                     /locus_tag="WGR46_000572"
     CDS             24548..26005
                     /locus_tag="WGR46_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0855572.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(26046..26435)
                     /locus_tag="WGR46_000573"
     CDS             complement(26046..26435)
                     /locus_tag="WGR46_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855573.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(26583..26972)
                     /locus_tag="WGR46_000574"
     CDS             complement(26583..26972)
                     /locus_tag="WGR46_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855574.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIGDIDNIVDFVSSYSLPKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            complement(27094..27483)
                     /locus_tag="WGR46_000575"
     CDS             complement(27094..27483)
                     /locus_tag="WGR46_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855575.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVEIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            complement(27584..27982)
                     /locus_tag="WGR46_000576"
     CDS             complement(27584..27982)
                     /locus_tag="WGR46_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855576.1"
                     /translation="MLGLTRDNCYDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQGFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            complement(27985..28176)
                     /locus_tag="WGR46_000577"
     CDS             complement(27985..28176)
                     /locus_tag="WGR46_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0855577.1"
                     /translation="MRLSDGIFDTKQVTKGVFDGKGEAKIPSYTWHHHQDTGRMQLIS
                     ERKHTQTGHIGGEAMDKGK"
     gene            complement(28237..28638)
                     /locus_tag="WGR46_000578"
     CDS             complement(28237..28638)
                     /locus_tag="WGR46_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855578.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEENNSDGNPTANYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(29055..29384)
                     /locus_tag="WGR46_000579"
     CDS             complement(29055..29384)
                     /locus_tag="WGR46_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855579.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(29410..30117)
                     /locus_tag="WGR46_000580"
                     /pseudo
     CDS             complement(29410..30117)
                     /locus_tag="WGR46_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            complement(30252..30794)
                     /locus_tag="WGR46_000581"
     CDS             complement(30252..30794)
                     /locus_tag="WGR46_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0855580.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLNNEVDNLSDMKRKPFAW"
     gene            complement(30921..31148)
                     /locus_tag="WGR46_000582"
     CDS             complement(30921..31148)
                     /locus_tag="WGR46_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855581.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(31207..31530)
                     /locus_tag="WGR46_000583"
     CDS             complement(31207..31530)
                     /locus_tag="WGR46_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0855582.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(31533..32240)
                     /locus_tag="WGR46_000584"
     CDS             complement(31533..32240)
                     /locus_tag="WGR46_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0855583.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            complement(32374..32691)
                     /locus_tag="WGR46_000585"
     CDS             complement(32374..32691)
                     /locus_tag="WGR46_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855584.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(32694..>33302)
                     /locus_tag="WGR46_000586"
     CDS             complement(32694..>33302)
                     /locus_tag="WGR46_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233664.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0855585.1"
                     /translation="PAEGKFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVF
                     NVAAAAKVKNLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKS
                     IGTKIHDGAQGKHISGHGNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVV
                     DFGYPIGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         8195 a         9379 c         7591 g         8137 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacctgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttatcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acgcggtcgg catcggtaaa acgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccgagtgcct cggtaacgtt ttgattcaaa cggtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaaaccgg gcgtatcgac aaacacaaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtaatac
      901 tgattttctg accgatgaga tgattcatca gcgttgattt gccgacattc ggacggccga
      961 caatcgctac gaagccgcaa cggtatccgt cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat atttgctttc gtatccatat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgct
     1141 tcctgctccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaatgacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aggcgaaacg gcgcgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc gactcgccgg acgcgttctg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc cgcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatcg ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcaagaa
     1921 tcgtggtaaa atccgcaagt tttcccccaa cgtattcccg caatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgcatc gccaccaaca ccgtcgccaa acaaaaccag
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgacccgg acaacttcag catcgtcagc
     2161 ctcgccgata tcaccaaaac caaccctgaa accgccgcac tgctctccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaacctaag
     2281 gactgaccct tcagacggca ttccgaaacc tccgaatacc aaaggacact caatgaacac
     2341 catcgccccc aacctcgacg gcaaacacct ccgcatcggc atcgtacagg cacgcttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacttt gcctcttctg aaaaatttga cgcactgatt gccatcggcg tcgttatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcagggg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accacggaaa acgacgcaca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tagaatgcgc
     2761 caacctcgtc aacctcctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccgata cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgag attgctaaaa acatccgcga aatgcccgac
     3001 tttgccaagg cagacgaaga attgttcaac aaacttttct tcggcacgca aaccaatgcg
     3061 gcagagtaca tccgacaaat ccgccccctg ctcgaccgcg acgaaaaaga cctcaacccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca tcatcaacga agccatcgaa gtaaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgtcc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca ggtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct ggtcgcatcg aatttccgga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgttgacgt atcaatcagt caacaacaca aatgccgtct gaaaaacctt tcagacggca
     3661 ttttgtttcc agccccatca atactgtacc gtccagccga tttcgccgcc cgcgcgcatc
     3721 gggacaagtt cgccgtcgcc gtagggaata cttgcgggga cggtttggct ttgtttgacg
     3781 agggtgatcg tgtcggtatt ttcaggaatg ccgtaaaacc ttgcgccatt tttcgaggca
     3841 aaggcttcga gtttgtccaa cgcgcctgct ttttcaaaca cttcggcgta aagctcgacg
     3901 gcggtcatcg cgctgaacat accggcgcaa ccgcaggcgt tttctttggc ggatttggcg
     3961 tgcggcgcgg agtcggtgcc gaggaagaat ttatgcgcct tctcgccggt aacggcggca
     4021 accaatgcct gacggtgggt ttcgcgtttg agtacgggca ggcagaaatg atgggggcgc
     4081 acgccgccga ccaagaggtc gttgcggttg agcaggaggt gttgcggggt cacggaggcg
     4141 gcaacgttgt cgcccgcttc caaaaccagg cgggcggctt cggcggtgct gatgtgttcg
     4201 aacacgactt taagattcgg cacttgcgcc aaaacgggtt tcatcacgcg cccgataaag
     4261 gcggcttcgc ggtcgaagat gtcgatttcg gggtcggtta cttcgccgtg aacgaggaac
     4321 aaaatgccct gtttcgccat ttcttccaac acggggatga gcttgaacag gtcggttacg
     4381 ccggaatcgg aattggtggt cgcgccggca gggtagagtt tgaaggcgac gatgccggcg
     4441 gctttggctt cgcgcacaag tgcaggcgtg gcttggtcgg tcaggtaaag cgtcatcaac
     4501 ggctcaaacg cgctgccttc gggcaatgcc gccatgatgc gcgctttgta ggcaagcgcg
     4561 tcggcaacgc tgacgacagg cggtttgagg ttgggcataa tgacggcacg ccccatttgg
     4621 cgggcggtat aaggagcaac ggctttgagc gcgtcgccgt cgcgcaggtg caggtgcata
     4681 tcgtcggggc ggataatggt cagggtttgc atggttttcc tttgttttat ttgaatgttc
     4741 agacggcatg ccgactttat gccgtctgaa gttaggggag aattaggggt ttcccgttgt
     4801 gggaacggtg cgtcaggttc aaaactgcct gtacgggtaa atccggggaa tcggcggttt
     4861 gtgcggtcaa tacggtttct tcggagctgc ccgcttcccg ccaggcatag ctgacttggc
     4921 ggatttcaaa tgatgcctgc accgactgtt ccgccctcaa ttctatattg gcatttttcg
     4981 gcggctgtcg gacaagtccg tcaaaatgtt ccaacagcgc ggcactgtct gcccgctcgg
     5041 ctttcacact gcaactgtcg cgcgtcaggc tcaaataata aatgccgcca tcgcgttcaa
     5101 gcgtccacaa cgcctgtaca tcgggttctg caacagtggg cggcagatgg gacaggtctt
     5161 cctttccgac agggacaaaa tgtccgacag cggcaaactt ggaaaccgat tcaaaactgc
     5221 cttgagtcag gacgcaggca ctccgaaaca aatcgacggc tacacgcgcg aaatcctgat
     5281 gccgtacacc ggattgccgg ttttgggcat tacacgcagc aagcacaaat gccgacagca
     5341 ataacatcaa tcgggacatc cgcatttcag acggcattta tttgtgttgg acgaccagcg
     5401 tgaacacgcc gtcctgttcg gaagcatcca acagcacatg accggtttgg cggcaaaaag
     5461 cctcaaaatc ccccggtgcg ccgccgtcgg ttgccagaac ggtcaacacg tcgccctgct
     5521 gcatttgcgc caaagccttt ttcgcccgca aaatcggcag gggacatttc aatccggtta
     5581 cgtctaaagt ttcgctgttc atcgtttgta ccgtttaaaa aatgggaatc ggggggagat
     5641 tataccccag ttgcttgcag ccccctttac caacccataa aataacgccg ttttcaattt
     5701 ggagagatct atgcgccgcg ccatccttct gattctggcc ctgactgccg gcacgtccct
     5761 tgccaccagc ttcagccaaa aagacacgcc gatcaacacc cgctaccgcg aaacgcccga
     5821 agaagccgcc gcccgcgaat ttaaagaaca caccgccaaa ctgccgccgc tgcccgatac
     5881 gcactcggac ggctggtttg acatttatgt ggatgaaaat tacggcaaac agccgaaaat
     5941 cctgctcgac agcctgcaaa ttatgcccgc gcccgacggc agcatccgct acattctcaa
     6001 tatccgctcc gacaaaggct acgacaacct gactgccgaa ggcatattct gcgcccgctt
     6061 ctccatcagg ttcggcaacg acaaactttc ctcctacaaa gtgttcggct acggcgacac
     6121 ggtcaaccgc cgctggatac agccccgaaa cgccgaatgg aaaccgatag gcggtacatt
     6181 aagccgcaac gacgcactgc gcaccgtcct gtatcaggca ttctgcgagg gcggtgtacc
     6241 cgccgacacg aaaggtttgg tacaacgcct gaaagaacgt gccggacgct atgccccgtc
     6301 gataaagccg cacgacaaat aaccagacac aaaaataccg tctgaaacat cggtttcaga
     6361 cggtattcga ttcatcagcc atcaggcggc agaaactttg tcctgacggc acaacagcgt
     6421 aatcaaatca ctgatgcgcc gcttcatatc gcggcggtcg acaatctggt cgattgcgcc
     6481 tttttccagc aggaactcgg cgcgttggaa gccttccggc agcgtttcgc gcaccgtctg
     6541 ctcaatcacg cgcggaccgg caaaaccgat cagcgcgttc ggttcggcaa gcacaacatc
     6601 acccaaaaat gcgaagctgg cggatacgcc gcccatagtc ggatcggtca acaccgatat
     6661 aaacggcagg cgtttttccg tcagcaaatg cagcgcggca ctggttttcg tcatctgcat
     6721 caatgagttc acaccctcct gcatacgcgc accgccggaa gccgccacac agacgaacgg
     6781 acaattgtcg gcaaccgccc ggcggacgcc ctgtacgaat cgttcgccca caaccgaacc
     6841 catcgaaccg ccgataaagc ggaactcaaa cgcagcaacc acaacgggca gaccgttcat
     6901 cttgcctttc attaccacca aagcatcatc ttccccggtc agcttgcgtg ccgcactcaa
     6961 acgatccgga tattttttgc cgtctttaaa cttcaaagga tctgtcggtt tgacatttcc
     7021 ggcaacctcc tctctgccat cctcatccaa aagcagattc aggcgttgtc gtgccgacaa
     7081 cggattgtgg tggttgcatt tcggacaaac ctgattgttc tgctgcaact cggtcgaata
     7141 aacggttgcc gaacaagacg gacatttgtg ccacaaaccc tcgggaacat tggaagaacc
     7201 gtctttgctg cggtttttga ttttgggtgg caggatttta tctaaccagc tcatggacaa
     7261 ctcctaaaat atttagaatg cgtgacagaa tcagcgcacg gcatccttca actctttgac
     7321 caaagcaccg acgaccgcag cctcgttgcc cgcattgttt tcaatctctt tcacaatccg
     7381 gctgccgaca atgaccgcgt cggcaacccg gccgattttg cgcacgcttt ccgcattgct
     7441 gatgccgaaa ccgacaccga tgggaatatc gatatactga tgcaaatact ctattttacg
     7501 cgaaacctca tccgtatcca aacttgccgc gcccgttacg cccttgagcg aaacataata
     7561 gacaaatccg cctgccagct cggcaatggt tttaatacgg tcttccgtcg tcgtcggcgc
     7621 aatcaggaaa atacagtcga ccccgttatc cttcagctcg cgatagagcg gatcgatggt
     7681 ttcgacaggg gaatccaccg tcaacacgcc gtccacaccc gcctttgcgg cttcctgagc
     7741 aaactcccga taacccatct tatgtacagg gttcaaatat cccatcaaaa caaccggcgt
     7801 ttgcgtgtcg gtttcacgga attttctgac gacatccaag acatcgcgca gcgaaatccc
     7861 gtttgccaac gcccgctccg ccgcacgctg aataaccggc ccatccgcca tcggatcgga
     7921 aaacggcacg cccaactcca aaatatccgc accgtttgca accatgccgt gcatcaatgc
     7981 caaagttgtc cgaatatcgg ggtcgcccac cgtaatatag ggaatcaatg cctttccgcc
     8041 atccaaagcg gcaaaagcct gccggattct gctcatttgt ctcttccatc ctatcggcaa
     8101 atatgccata aaacacaatc agtaaatttt tcccagtata acatttgcgg tacggatagg
     8161 cgaatacggc aaacagaaac cacgcaaatc agagccgccg tttgaacgag gcggaaataa
     8221 acggcaagca tctcggatta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     8281 agacagtaca aatagtacgg agccgattca cttggtgctc cagcacctta gagaatcgtt
     8341 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataacaaaat
     8401 tcagaatcaa gacagggcaa taccatactg gttttgcacc aacccgctat aaaattgcat
     8461 aaaaaaaata gacgctgtaa aacacagcgt ctatttttta tctggcggag taagagggat
     8521 tcgaaccctc gatgcaggtt gaccccacat gcttccttag cagggaagtg ccttcagcct
     8581 ctcagccatt actcctaagg aagctgtaac tataatcaac ttttccgata ccgtcaagga
     8641 aaaatggaaa gcaaatatta atcttttgaa ataaaataag atttaccctg attataccca
     8701 atcaggcact tcttttcttc ctgaatggaa atattcctga ctgccacatc ctcaagcaaa
     8761 atgcttttgt gcatcctgtt tgatttcctg aaaccacgcg tcttcaggaa aacgtgcaag
     8821 cgcgacatcc aatgttgaca gcaggttttg cgacagggct ttggtcaggt attcctcctc
     8881 gttggcttct gccgctttgt cgggctgttg tgccaaaccg tcggcacggg ctttgtgtcc
     8941 gtgtgccaaa tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
     9001 cagatagtac gacaaggcga ggcaacgccg tactggttta aatttaatcc actataattg
     9061 agcgcggtaa ttttttgcgc ccaaagctgc ggtatgtcgg gattgtcttc aatcatgacg
     9121 gaaaggatgg atacggcggt cgccagcctg ccgccgttca ttctaaggtc gagggaacgg
     9181 ttgggcggca gggtgctgag tgtttctgcc attctgaacc aaaattcgcc actggcttcg
     9241 tcgggtgcgt tcaatttaag catttcatag tgaacctcct gatcatcgtt ttgcacgaga
     9301 ccccatactt cggcaaggat tttctgttct tcccggctca agtgttgcca cttttcaata
     9361 tgttgctgca tatcttttcc ctttgaacct gttttatacg gtctgaaata cagcgcgaac
     9421 cctatccaaa tcttcctgcg tatccacacc ggcggcgggg gcttctttgg cggtttcgac
     9481 ggcaatcggg tagccgtgcc acaggacgcg cagctgttcc agcgattcga tggtttccag
     9541 cggcgaaacg ctcatttcgg catagcgttg caggaagccg gcgcggtaag cgtagatgcc
     9601 gatatggcgc aggacggcgg tttcagacgg catttcgcgt tttcctgcac gcatcgcatc
     9661 gcgcggatag ggaatcgggg cgcggctgaa gtagatggcg ttgcggtttt tgtcgaggac
     9721 gactttgacg gcattgggat tcatcaattc gtcgaaatcg tgcaattcgt gtgccgcagt
     9781 tgccatctgt acgttgtttt ccaccaagac ttcggcagtg cggtcgatga gttcgggggc
     9841 aatcagcggc tcgtcgccct gtacgttgac aaccaccaga tgcggcggca gcttcagcgc
     9901 ggcagaggct tcggcaaggc gcgtcgtgcc gctttcgtgc cggtttgaag tcatgacgac
     9961 ttcgacgccg tgcgcctgac aggccgtctg aatatcggga tggtcggtgg caacgacgac
    10021 gcgcacggct ttactttttg ccgcctgttc ggcaacgcgc acgaccatcg gtttaccgtg
    10081 gatatccgcc aaggcttttc cgggcaggcg cgatgaatcc agccgcgccg gaatcaatac
    10141 gacgaattca gtcatgcttt gagttcctct tcgctcaacg ctcgcgcttc gttttccagc
    10201 atatagggaa tgccgtcttt aatcggatag gcaagcttcg cctgacggct ccacaattcc
    10261 tgtttgtcct gatgatattc cagcctgcct ttggtaacgg ggcagacgag gatgtctaag
    10321 aatttttttt ccatagggtt gctttcgggt ttgatggttc aatcaatcgg atgccctctt
    10381 caatatttca gacggctatc atttatttct gatgcctttt cccaacctgt ccggcttgtt
    10441 tacagcccgc atcgggattt ttaccgccgc agatgatgcg ccgcaactct tccgtaaacg
    10501 cgggcagatg atgtcgcgcg attttccctg acaatgcagt ccaagatact gctatttcac
    10561 tcatcgattt cttgattaag cgcggatttt tggcaacgac ggactgaccg acaggcgaac
    10621 cgtaaaaggc aatcatgccg tcaatttctt cttgcgtgta tatctcacgg gcattcttca
    10681 ataaagtatt gcggacagcc tgtttcactt cgggcgtaat caaatctttc aaaacattct
    10741 cacgatagcg gttaaaggct tttgccgcct gatctttttt cgcttccggc atttcggcaa
    10801 gggctttgtc cgcatacggt ttaaatccgg cattaaagcc ctcaatcata tttttttcta
    10861 tatcccggtc aaaattctgc gtatccagcc aacgcgccaa cgacgcgtcg ctgggcggag
    10921 cggcaaaagc attggtgcac aatgccagcg tggcgaaggg caataacaag gttttcagtt
    10981 taatattcaa tcctttaatg aatgattaat gcagataaaa aatacgaccg gttttctcat
    11041 tcctgtccaa gtggaaatcc ggagatttgg gatttctgaa gcctcctagg cattcctgaa
    11101 aattcgagac ttagattccc gcctgcgcgg gaatgacggc gcgaggcgtt cttattcaaa
    11161 tccgtaatca ctccgcccaa accgtgcttt cagacggcct tcggtacatc ttccaaccgc
    11221 tccaacacaa acgccgccaa atcaggttcg attatcgcac aaacgggcaa cacccaaaca
    11281 ttatcggtgc aaatgccgtc tgaaaatttg accgcatctt tctccgtaat aatgaccgcg
    11341 tccgcatcgg gcaaatctgc cgccgaaatg tcggcgtggt cgggcagcgc gacggttcgc
    11401 ttcacggtaa tgcccatatt ccgcaacgaa tcaaaaaacc gcgccggctt ggcaatacct
    11461 gccacggcga cgacggtttg atttttcaga cggcctgtgt ccagtatttc ggacggattg
    11521 ttcaaacggt aaatccgtcc cgcttcgata cggctgtgaa acatattttc agacggccta
    11581 aacagcgcat ccgccttgcc gccgctgacg acgaccgcat ccaccgaatc cagccgcaac
    11641 aaaggttcgc gcaaactgcc gttgggcagt aaatccaaat ccgtgcgccc cgtatccgcc
    11701 gccggaaaca ccgcgatttc cacatctcgc cgcagggcgt aatgctgcaa accgtcgtcc
    11761 gccacaatca gtccgatgtc gggatgcgcc gccagcaacg ccctgcctgc ctctgcacgg
    11821 ctgctgccca ccgccgtcgg cgcaacggtt ttgcggaaca gcagcaaagg ctcgtcgccc
    11881 gcatcttccg cccggctctt ctcattcaat acatgaaccg ccttgctctt gcgcccgtag
    11941 ccccggctga tgatgccgac cttgacacct ttttcctgca aacccgacac cagcgcggca
    12001 acaatcggcg tttttcctgt accgccggca tggatgttgc cgaccacgac cacaggcacg
    12061 ggcagctttt cgctttgccg ttttcccgat aaaaaatccg tccgccgttt tgccgcaatt
    12121 ttggcaaaca gcccggagag cggcttaagc agaaaagaca aaaccggata aggtttttgc
    12181 caatgccgtt cgataacggt atgaaatttg aaacaattcg gcatattcga tacaaatcct
    12241 cgaagcaaaa tcagcccggg tcattaatca tgcgtacccg atacagaccc acctataaac
    12301 aacgcgaaga ccaaaccgat aagcgaacca atcaggtaaa taagcaactg cgctttacag
    12361 aaattggcgc ggttcgggtt gcctctgccg aacacccaaa caaaaattga ccaaaggaat
    12421 catgaaaaca atcatcgtca acacccactg ccctgtcgtc atgacgggag gcggtttgtt
    12481 ttgaagtctc gggcatatcg ccaatatgat aacggacagg tttcatctct ttcattttcc
    12541 agtatctttc atttatagtg gattaacaaa aaccagtacg gcgttggctc gccttagctc
    12601 aaagagaacg attctctaag gtgctgaaac accaagtgaa tcggttctgt actatttgta
    12661 ctgtctgcgg cttcgtcgct ttgtcctgat ttttgttaat ccactatatc aatctgacag
    12721 caaaatgccg cctgaaaaca gttcagacgg cattttaaac cgcaaacggc ttacagcttc
    12781 cattcgctca gcttggcagc gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg
    12841 ctttccatcg ctgctttggc ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac
    12901 ggagtcggaa tcaggtattc cgcgccgaat tcaaatttct taccgtaagc ggcaaccact
    12961 tcttcggtta cctcttccat cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt
    13021 tcttcgttga tggtcgtcgc gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac
    13081 aatacattgt tcacttggtt cgggaagtcg gagcggccgg taccgataac cacgtccgga
    13141 cgggtttctt tcgccagcgg cggcaggatt tccggattcg ggttggccat agcgaacacg
    13201 atgggttttt cgttcatggt gttcagtatt tcaggcgtca gcaggttcgc gccggagagg
    13261 cccaagaaga tgtctttacc tttgacggca tcggcaagta cgcgctggcc attgtcttca
    13321 acggcgtaga attttttgga ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc
    13381 acgcctttgg agtcgcaaac ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg
    13441 ttcaagcagg caatcgcggc cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt
    13501 ttacggccgg taaaacgcag ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg
    13561 tgctggtcgt cgtggaatac ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag
    13621 aaacactcgg gtgctttgat gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg
    13681 gcgatgatgt ccacgagttt ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc
    13741 acaccggcga attttttgaa caatacgcct ttgccttcca tcacgggttt gcccgccagc
    13801 gcgccgatgt cgcccaagcc caaaacggcc gtaccgttgg aaatgacagc gaccaagttg
    13861 cctttggcgg tgtatttgta ggcattttgc ggatcggcat ggatttccat acaaggagcg
    13921 gctacgcccg gagagtacgc caacgccaaa tctttgtcgg tcgccagaga tttggtcggg
    13981 gtaacggaaa ttttgcccgg aacgggtaat tcgtggaact tgagggcggc ttctttcaat
    14041 gagttttcca tgtgtcaatc ctgttgcgag aaaaagattt gaatcggact gtccgaaacg
    14101 ctttcgggtc atcatgttac gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa
    14161 ccgagcgtaa ttctatcatg ccgaaagttt ggcgcatacc gccattttca cctgcggcac
    14221 tgccgtttga ttttcggcgc gggcggatgc gcttgtttca atatttgccg catcaacagc
    14281 cgaaatgtct gtccaacact ttttctatgc tgtttctgac ttcatccaag ccgaggttac
    14341 tgtcgataac ggcgtaacgt tccggacagg cggcggcacg gttcagataa acgctgcgca
    14401 cacgcatgaa gaaatctgcc tgttcctgct cgaaccggtc tttctcgcgc gtctgcccga
    14461 tacgcgccat cgacacttcc agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac
    14521 cgccctgcac ccaatgttcc aaaatttcaa tgtcttcaga cggcattccc cgcccgccgc
    14581 cctgataggc gaaggtcgca tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa
    14641 gtgcaggcaa tatcacgtct tcaatatgct gcattctggc ggcgaacatc atcagtgttt
    14701 ccgcacgcaa accggctttg gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga
    14761 ccggcgttcc gcccggttcg cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg
    14821 ccttgatgac ggcaaggttg gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga
    14881 attgcggttt catgcttgca gttccggtaa gggtatgccg tccgaaaaca caaacttttc
    14941 agacggcatg gtttattttt tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa
    15001 atcatggctg aactggctca agcccgtacc gtccattttg gacacgaaat acaggtattt
    15061 ttcaccggac ggatgggcgg cggcatcgag tgccgccttg ccgggcagcg cgatcggggt
    15121 tggcggcaga ccgccgcgcg tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt
    15181 acggattttg cccttgtatg ccgcacccat gccgtaaatc acggacgggt cggtttgcag
    15241 gcgcataccg attttcaggc ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc
    15301 atgccctgtt tccttttcga tcaggctcgc cataatcagc atttcataag gatttttata
    15361 aggcagcccg tcctgcctgc tttcccatgc ctcattcagg cggcgttgca tcgccttgta
    15421 ggcggtttgg taaatccgta aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa
    15481 aaactgccct tcaggattgc cgctgaaggc atcaggggca acttccgcca tcagtttttc
    15541 attgctccag cctttggtgt cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat
    15601 atgcgaaaaa cgcgaacctt cgataatctg cacggtaacg gaatccggcc tgccgccgcg
    15661 cattttctgc aagatatccc aagcagacac ttccgaaggc agtctgtacg tccccgtatg
    15721 cagcctgttg tgcacaccca aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac
    15781 gatgcggtct tcggcaagtt tcctgccgac cgacgaaata ccctgatttt tggcaatctt
    15841 gattcggtat gccctgccat tatccttagg aacaaaaagc agcgcggcga aaacggctgc
    15901 cgacacggtc aaaaaaacgg cagaccattt caacaatttt ctcaacatgg taggattccc
    15961 agcattcgga atacgtctta aaattcgggc gacagtataa cccaaattgc ccgccggaca
    16021 ggaagaacat catggacaat cacgccgaag cacactggca aaacggctgg cttcaaagca
    16081 tacgccatac cccgtcgccc aatttcagcc cgagggaaac gggggaaacg gtttccctga
    16141 tcgtgttgca caacatttca ctgccgccgt tcgaatacgg cacggatgct gtggaaaagc
    16201 tgtttgccaa ccggctcgac cccaacggac atccgttctt cagcctgata cacactttgc
    16261 gcgtatccag ccatttctta atcaaacgcg acggagaaac ggtacagttc gtttcctgcg
    16321 acgacatggc gtaccacgcc ggcgtgtctt cattcggcgg cagggaaaaa tgcaacgcat
    16381 tttccatcgg catcgaactg gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc
    16441 gttcgctcga agccttgttg gacgcaatct gccgccgcta ccccatcacg gcggtaacgg
    16501 ggcaccaaga catcgcgccc ggccgcaaaa ccgaccccgg tcattttttc gactggcggc
    16561 ggatacgggg gaaagggttt cccgtggaca gaaatgccgt ctgaaaacca cagattttag
    16621 acggcatttc ggaaaatttc cgcttatttc gacacactgc tgtcagaccc tgcaattttc
    16681 gttataattc aaacgttata accggccgtt ccgtcagact gccggaaacg caagcccgat
    16741 ttaaccgcag tacccgcact ttgcgcatcc tgcatcccga aatttaaagt gattgccatg
    16801 atttacatcg tactgttcct cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag
    16861 gaaaaccaat accgcaaaaa agtgcgcgac cagttcgggc actccgacaa agatgccctg
    16921 ctcaacagca aaaccagcca tgtccgcgac ggcaaaccgt ccggcgggcc agtcatgatg
    16981 ccgaaacccc aaccggcggt caaaaaaacg gcaaaacccc aagaccccgc catgcgcaac
    17041 ctgcaagaac aggatgccgt ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc
    17101 aaaaccgaaa tcgaaaccgc cttggaagaa agcggcatta tcggcaactc cgcccacacc
    17161 gtttccgaac cccaaaccgg acattccgca ccgaaacctg ccgacgcgcc ggcaaaaccc
    17221 gttcccgttc cgcaaacgcc ggcaaaaccg ctgattacgc tcaaagagct gtcgaaggtc
    17281 gagctgccct ggtttgacgt gcgcttcgac ttcatctctt atatcgcgct gaccgaagcc
    17341 aaagaacttc acgcactgcc gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc
    17401 accatggacg accatttcca gattgccgaa cccatcccgg gcatccgcta tcaggcattt
    17461 atcgtgggta ttcaggcagt cagccgcaac ggacttgcct cgcaggaaga actctccgca
    17521 ttcaaccgcc aggtggacgc attcgcacaa agcatgggcg gtcagacgct gcacaccgac
    17581 cttgccgcct ttatcgaagt ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag
    17641 accatcgcca tccatttggt ttccccgacc agcatcagcg gcgtagaact gcgttccgcc
    17701 gtaacgggcg tgggtttcgt tttggaagac gacggcgcgt tccactatac cgacacgtcg
    17761 ggctcgacca tgttctccat ctgctcgctc aacaacgagc cgtttaccaa tgcccttttg
    17821 gacaaccagt cctacaaagg cttcagtatg ctgctcgaca tcccgcactc tccggcaggc
    17881 gaaaaaacct tcgacgattt gtttatggat ttggcggtac gcctgtccgg ccagttgaac
    17941 ctgaatctgg tcaacgacaa aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc
    18001 acttatgtat tggctcgtca gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa
    18061 accgcattgc gcctgttctc ctaaaatcga atgcaatcaa agcggatatg tcgttacggc
    18121 atatccgctt tttttcgggt aaaattcagc tgttttcaac ctatcatttt tcagacgacc
    18181 tttgcccgtt tcatcccgta tcagacaaag gtcgtctgaa aaccttctct catgaatcca
    18241 aactcaaaac ataacactaa ctttaccaat cttcttaaac cgtctgatag tgatataaaa
    18301 caatttgccg cacaacacat ccgccacctc accgacctgc tcaaccgcta tgcctacgaa
    18361 tactacaccc tcgacgcgcc cagcgtaccc gatgccgaat acgacaaatt attccgcgag
    18421 ctcgaagcgt tggaattaaa ccatcccgag ctcaaactgc ccgacagtcc gacccagcgc
    18481 gtcggcggcg agcctttagc gggatttgcc gaagtgcgcc acgaagtgcc gatgctgtcg
    18541 ctgaccaacg ctttttcccc gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac
    18601 gctttcgacc aacgcgtgcg cgacggtttg gacggcggca aacccgaata cgttatcgaa
    18661 cccaaattcg acggcctcgc catcagcctg ctctaccgcg acggcgtatt ggtacaggcg
    18721 gcaacgcgcg gcgacggcac aacgggcgaa gacgttaccc aaaacatcaa aaccgtcgcc
    18781 aacatccccc tgcggctgca cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa
    18841 gtactgatgc aaaaagccga ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa
    18901 aaaccctttg ccaatccgcg caacgccgct gccggcagcc tgcgccaact cgattcgcgc
    18961 atcactgcgc aacgcaaact gcactttttc ccctactccg tcgcccgcca gcaaggcggt
    19021 ttcgtcgcgg aagaacacat ccaagaactc gcctatttcc aagcactcgg cttcagtctg
    19081 ccaaatggca atttcggctg tttcaaaaat atcgacgaag tattggcgtt ttacgaacaa
    19141 atgcagcaaa aacgccccga actgccctac gaaatcgacg gtatggtggt caaagtcaac
    19201 agcttggcgc aacagcgcga actcggcttc atctcacgcg cgccacgctg ggcgattgcc
    19261 cacaaattcc ctgccgaaga agccctgacc attgtcgaag cgattgacgt gcaaatcggc
    19321 aggacgggcg cggtaacgcc ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc
    19381 gttaccaacg ccaccctgca caatcaggac gaagtatcgc gtaaagacgt gcgcgtcggc
    19441 gataccgtcg tcgtgcgccg cgccggagac gtgattcccg aagttgtgcg cgtgattttt
    19501 gaacgccgcc cgatgcggga aaccgccgtt gccgtttcag acggcatcgg gcatcggcag
    19561 gacgatttgt ttgccgaaac accgtccgct aatcaaaccc aatccgttcc gctccacaag
    19621 ccctaccgcc tgccgaccca ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggc
    19681 gaagccgtcg cccgatgcag cggcggcatg ctttgtcagg cacaacgcgc gcaaggctta
    19741 atccacttcg cctcgcgcaa agcgatggac atcgacggct tgggtgaaaa acaaatcgaa
    19801 cagcttgtcg cccaagacct cgtccgccac ttcgccgacc tctaccgcct cgatattccg
    19861 accctgcaaa aaatgaagga agcggcggac aaaaccgttg ccgaatcgga tcaaatgccg
    19921 tctgaaggca gttcggtcgg tgcgtcgggc aaacataaaa aacaaccgac caagtgggcg
    19981 gaaaacatcc tcgcaggcat agaatccggc aaaacgcccg aactcgcgcg cttcctgttc
    20041 gcgctcggca tccgccacgt cggcgaacgc accgccaaaa cgctggcaca ggcattcggc
    20101 acattggaac gcgtccgccg cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt
    20161 acggtagtgg cgcgttccat tgcccacttc ttcgcccaag ccgaacagca ggcgatgata
    20221 gacgagctgc tcgccgcagg cgttgccccg caagcccaag ccgtcagcct gcctgccgca
    20281 caatatgccg aaccgcaacg ctggattgcc cgcctgcccg gtttcaaaat tagcgaaaac
    20341 aaagcccaag ccttatggga actcgccggc aaaagtatag agggtctgca aaccgacaaa
    20401 tccctccccg ccgactggca agcatggcgc agcgaaccac aaaacgccgc cctactcgaa
    20461 aacctgaaaa ccttcttcgc gcaaatgccg tctgaagacg aggcggcgca aggttcagac
    20521 ggcatcaata aagccgtagc gggcaaaacc ttcgtgttaa ctggcacact gcccaccctc
    20581 aaacgcgacc aagcccaatc cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtg
    20641 tccaaaaaaa ccgattacgt cgtcgccgga gaagccgcgg gcagcaagct ggaaaaagcc
    20701 aatgccttgg gcgtttccgt cctcagcgaa gccgaactgc ttaccctgct cggctgaacc
    20761 catgccgtct gaaccgcctt cagacggcat tgcccacttt tccgaaccac accatgcaca
    20821 ccatttcctt tccaaaccgc acccgactca ccgccctgcc gcccctgtcg ctttacatcc
    20881 acatcccgtg gtgcatcaaa aaatgcccgt attgcgactt taattcccac agcctgaaaa
    20941 acggattgcc cgaagccgcc tatatcgacg cgctgctgac cgacttgcag ctcgaattgc
    21001 ccaatatttg gggcaggccg gtggaaacga tctttttcgg cggcggtacg cccagcctgt
    21061 ttcaggcgga atcgattgac cgtttattga gcggcgtgcg ttcgctgttg cgcttgcagc
    21121 cggaagcgga aattaccttg gaagccaatc cggggacatt tgaaatcgag aagtttcaag
    21181 gatttaaaga cgcaggcatc acgcggcttt ctattggtgt acaaagcttc aacgacgata
    21241 tgctctcaag gctggggcgc gtccacaacg gtaaagaagc cttaaccgcc atagctactg
    21301 ccttgaaatt atttgataaa atcaatatcg acttgatgta cgccctgccg aaccaaaccg
    21361 ttcggacggc attggatgac gtacaaaccg ccatcgccac gggcgcaacc cacatcagcg
    21421 cgtatcacct gacgatggaa ccgaacacgc cgttcggaca tacgccgccg aaaggtttgc
    21481 cgcaagacga agccgccctc gacatcgaag acacggtaca cggcgcattg gaaaacgccg
    21541 gctttataca ttacgaaaca tcggcttttg cgaaacccgc catgcggtgc cgccacaatt
    21601 tgaactactg gcagttcggc gattatttgg gcatcggcgc gggcgcgcac ggcaaaatct
    21661 cctatcccga ccgcatcgag cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct
    21721 taatgcaaag ccaaccgagt gaagccgtcg aacgcaaaac cgttgccgcc gaagatttgc
    21781 cgttcgaatt catgatgaac gccctgcgcc tgaccgacgg cgtacccacc gcgatgttgc
    21841 aggagcgcac gggcgtaccg agtgccaaaa tcatggcgca aatcgaaacg gcaaggcaaa
    21901 aaggcctgct ggaaaccgac cccgccgtat tccgcccgac cgaaaaagga cgcttgtttt
    21961 taaacgattt gctgcagtgt tttttatagt ggattaacaa aaaccagtac ggcgttgcct
    22021 cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg
    22081 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
    22141 aagcgcaaac aaatcggcgg ccgcccggga aaacccgccc cgaacgcgtc cggaaaatat
    22201 gcttatcgat ggaaaacgca gccgcatccc ccgccgggcg tttcagacgg cacagccgcc
    22261 gccggaaatg tccgacgctt aaggcacaga cgcacacaaa aaaccgtatg cctgcacctg
    22321 caacaatccg acagataccg ctgttttttc caaaccgttt gcaagtttca cccatccgcc
    22381 gcgtgatgcc gccaccacca tttaaaggca acgcgcgggt taacggcttt gccgtcggca
    22441 aagcagccgg ataccgctac gtatcttgaa gtattgaaaa tattacgatg caaaaagaaa
    22501 atttaagtat aatacagcag gattctttaa cggattctta acaatttttc taactgacca
    22561 taaaggaacc aaaatatgaa aaaagcactt gccacactga ttgccctcgc tctcccggcc
    22621 gccgcactgg cggaaggcgc atccggcttt tacgtccaag ccgatgccgc acacgcaaaa
    22681 gcctcaagct ctttaggttc tgccaaaggc ttcagcccgc gcatctccgc aggctaccgc
    22741 atcaacgacc tccgcttcgc cgtcgattac acgcgctaca aaaactataa agccccatcc
    22801 accgatttca aactttacag catcggcgcg tccgccattt acgacttcga cacccaatcg
    22861 cccgtcaaac cgtatctcgg cgcgcgcttg agcctcaacc gcgcctccgt cgacttgggc
    22921 ggcagcgaca gcttcagcca aacctccatc ggcctcggcg tattgacggg cgtaagctat
    22981 gccgttaccc cgaatgtcga tttggatgcc ggctaccgct acaactacat cggcaaagtc
    23041 aacactgtca aaaacgtccg ttccggcgaa ctgtccgtcg gcgtgcgcgt caaattctga
    23101 tatgcgcctt attctgcaaa ccgccgagcc ttcggcggtt ttgttttctg ccaccgcaac
    23161 tacacaagcc ggcggttttg tacgataatc ccgaatgctg cggcttctgc cgccctattt
    23221 tttgaggaat ccgaaatgtc caaaaccatc atccacaccg acaaagcccc cgccgccatc
    23281 ggcgcgtaca gccaagccgt ccgagcaggc gacaccgttt acatgagcgg tcaaatcccc
    23341 ctcgatcccg ccacgatgac cgtcgtcggc aacggcgatt tccgcgccga agcgcgccaa
    23401 gtgttccaaa acctgcaagc cgtcgccgaa gcggcaggcg gcacgctggc cgacatcgtc
    23461 aaactcaacg cctacctgac cgacttgggc aattttgccg tcttcaacga agtcatggcg
    23521 gaatttatcg ccgagccctt ccccgcccgc gccgccgtcg gcgttgcctc gctgcccaaa
    23581 ggcgtgcagg tcgaagccga agccgtcctc gttttgaacg cataattcca cgttcggact
    23641 gcatgatatt tcaaaaccat gccgtccgaa ccgcaacact ttcccacttc acaacatctg
    23701 caatggcaca ttacgcaatc ggcgacatac aaggctgttt cgacgaactg accgcgctgc
    23761 tcggcaaaat cggtttcaac cacggcacgg acaccctctg gctgacgggc gacatcgtca
    23821 accgcggccc gaaatccctc gaaacgctgc aattctgcat ccggcacgaa aacagcgtgc
    23881 aaatcgtcct cggcaaccac gacctgcacc tgctcgctgt cggctgcggc gaaggcgcgc
    23941 ccaaacgcag cgacacaatc gaacccatac tcaaacaccc cgacggaaaa aaaatgctcg
    24001 actggctgcg tgcgcaaccg ctgttgatac gcgagggcag ccgcgtgatg gtacacgccg
    24061 gcatcctgcc gcaatggcgc ataaccaaag ccgaatcgct cgctagagaa gccgaagccg
    24121 aactgcgcgg caaaaaatac gtcaaattct tctccaaaat gtacggcaac aaaccggccg
    24181 cgtgggacga aggtttgaaa ggctatgccc gcctgcgctt catcgtcaac gccttcacgc
    24241 ggatgcgcgc cctgaccttt aaaaacgaac tggatttcga ctacaaatcc acagtgaaaa
    24301 aaatgccgcc ttacctgcgc ccgtggttca aagcccccga ccggcaaaac ctcgaccgca
    24361 ccatcatctt cggacactgg tcctcgctgg gctacacgaa tgccgacaac gtcatctcgc
    24421 tggacaccgg cgcgctgtgg ggcggacagc tgaccgccgt caacctcgaa acggaagaaa
    24481 ttacccaagt ccaagccgcc ggcggcatag actggaaaag cttcgcaaaa taacggaccg
    24541 ccccactatg cacaaaatcc tgcccatcgc ccacgtcctc tcccgactgg gtatgctgtt
    24601 ttcctttatc ctgctgatac ccgccgccct ctcctacgcc ttttcggacg gcgcgtacac
    24661 cgctttcgcc accaccgcga ccgttaccct ttccggctcg tgcatcgtcc ggctcgccac
    24721 cctccggttc aggcgcgaac tgcgcccgcg cgacggcttt accctcgtcc tgatgttgtg
    24781 gctggcgttt gccgctatgg ctgcgatgcc gatgtacctg tatttcccga atatgggctt
    24841 taccgacgca ttttttgaat cgatgtcggg actgaccacc accggcgcga ccgtcatccc
    24901 ccacgtcgac gggctcgccc cctccgtcaa cttttggcgg catatgctca actggctggg
    24961 cgggatgggc atcatcgtcc ttgccgtcgc catcctgcct atgctcggcg taggcggcac
    25021 gcagttattc aaagccgaaa tccccggcat tgacaaagaa agcaaaatgt cgccgcgcat
    25081 ttcccaagtg gcgaaaaaac tttggttcgg ctacaccctg atcaccatcc tcgcggcagc
    25141 ctgcctgcat tttgccggga tgggctggtt cgatgccgtc tgtcacgcga tggctaccct
    25201 ctcgctgggc gggttttcca cccacgatgc cagcatagct tattacaact cccccctcat
    25261 cgaagcggtc atcatcgttt tcaccattgt cggcggcatc aattttgcca accatttcgc
    25321 cgccctcaac agccgcagcc tcaaaaccta ttggaaagac gaagaatgcc ggacgatgct
    25381 gctgctgctc tccggcagca tccttgccgc cgccctgtac ctgtggcaca ccggctatta
    25441 cgccggtttt accgaatccc tgcgctacac cgccttcaac ttcgtctcca tcggactggc
    25501 aaacgggctg gccaacaccg acttcgcaca atggccgctc ctgatttccc tgtggatgtt
    25561 tttcctcgcc aacatcctcg ccaactccgg ctcgaccggc ggcggcatca aaaccatacg
    25621 cgccctcgtc ctgttcaaat tcagcctgcg cgaaatgatg gtgctgctgc accccaaagc
    25681 cgtccgcacc gtcaaaatca gcggcaaggc catacccgac cgcctcgcgc tgaccgttat
    25741 gtccttcatc ttcatctact ttatgaccgt cgtcctcttc agcttcctgc tgatggcgag
    25801 cggtatggaa tttaccaccg ccttcaccgc cgtcatcgcc tgcatcacca acgccggccc
    25861 cggcctgggc gaagtcgggc ccgccggcaa ttacgccggt ttggacgtga tgcagaaatg
    25921 gatctgcgtt accgccatgc tcttgggcag gctggaaatc ttcaccgtct tcatcctctt
    25981 cacccccgcc tactggaaga aataaaaaat gccgtctgaa aacttttcag acggcatctt
    26041 gtcctttaaa gaagcggaag ctctacaaaa ggaaagaaat gctgaaacct ctgataaatt
    26101 tcaggatatt tctctaaggc ttttaatgct ttttcttttg aaataggcgg atcatagaca
    26161 tctatccccc ttaagaaggc aatgcccgtt aatgcatttt tttgatcatt tgaaatgtaa
    26221 ccataattaa cgactccaat tacatcataa atttccccta tactcttatc aaaatcaatc
    26281 aaatcaaaaa tatacaatgg aattgtatca ataggagtat ctctgacaac tatatccaac
    26341 cataatttaa attcagaaag attaatagca tcatagaaca tacagactat tgcaaatcct
    26401 aaatcttcac aattttcttt actaattttc cacataatta tttaccttta tccattgctt
    26461 cccaaccgat acatccggta ttttagcttc tccttttccg tcaaacactc ctttggttac
    26521 ctgctttgtg tcaaaaatgc cgtctgaaag ccgaatatca tttcagacgg cattttgact
    26581 gtttaaagcg ggggcagttc tacaaacgga aagaaatgct gaaacttctg ataaatttca
    26641 ggatgtttct ttaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
    26701 atccccctta agaaggcaat gcctgtcaag gcattttttt tcgatttcgg caggctgtaa
    26761 ctcgaaacaa aatctacaat attgtcaatg tctccaattc ccccatcgaa atccgccaag
    26821 tcaaaaatat aaaaagggat gtcctcgatg ggcatatctc gtattacttg ttcaatccat
    26881 aatttgaatt catttaaatc aatagattga gagaataagc atttaattgc aaattctaaa
    26941 tcatcactat cctcttttat gattttccac ataattatct tcctttgttc atcattatct
    27001 tcctttgccg tcaaacactc ttttggttac ctgctttgta tcaaaaaatg ccgtctgaaa
    27061 gccgaatatt gtttcagacg gcattttgac tgtttaaagc ggaggcagtt ctacaaatgg
    27121 aaagaaatgc tgaaacttct gataaatttc aggatatttc tctaaggctt ttaatgcttt
    27181 ttcttttgaa acgggaggat catagacatc tatacccctt aagaaggcaa tgccggtcaa
    27241 ggcatttttt ttcgatttcg gtaggctgga actcggagca aaacctacaa tattgccaat
    27301 atcgccaatt tccacatcga aatccgccaa atcaaaaata taaaaaggga tgtcctcgat
    27361 gggcatatcg cgtattactt gttcaatcca taacttgaat tcatttaaat caatagattg
    27421 agagaataag catttaattg caaatcctaa atcatcacta tcctctttta tgattttcca
    27481 cataattatc ttcctttgcc gtcaaacgct cttttagtta cccgctttat atcaaaaata
    27541 ccatctgaaa gccgaatatc gtttcagacg gcattttgac tgtttaaagc gggggcagtt
    27601 ctacaaacgg aaagaaatgc cgaaacttct gataaatttc aggatgtttt tttaaggctt
    27661 ttaacgcctt ctccctagta atcggaatat cgtaaaaccc ttgattccgt atataggtaa
    27721 tgccatatac ggcatctttt tcctttttcg tcataccggt agttggaaca aagccaataa
    27781 tattatatat atttatggaa caatcatctg attccaaaca atctattaaa tcaatcagat
    27841 accttggaaa actttcggca gggacagtcc tgacaattcc atataaccaa tccaagaatt
    27901 cttttttgtc ggctgcattg cgctcataac acaatatagc aaatcccaaa tcataacaat
    27961 tgtctcgtgt aagccccaac ataatcattt tcctttatcc attgcttcac cacctatatg
    28021 ccctgtttgc gtatgctttc tttcggatat taactgcatt cgtcccgtat cctgatgatg
    28081 atgccaagta tagctcggta ttttagcttc tccttttccg tcaaacactc ctttggttac
    28141 ctgctttgtg tcaaaaatac cgtctgaaag ccgaatattg tttcagacgg cattttgatt
    28201 gttgggtttt taatcagagc tttgtttgat aatttgttat tttgaataaa atacaggttc
    28261 tgtcgagctg ataacttcta ttacagaatc gctaaaaaag aaaatataag ccttataata
    28321 tttcttttca aaaaaatcaa aactttcttc tttcaaccag tccatataac tgtcatctat
    28381 tatctcgaaa aaataatttg cagtaggatt cccatctgaa ttattttctt ctttattttc
    28441 aaataaatat tctgaaaatt ttactttata agattcatct gttatttgaa aatgaattat
    28501 attgtcaata aatatttcaa tatttaattc ataatttcgg tcggatgcca aaatgttcac
    28561 atcttgttcg gtaacacaaa ttttgtctac gtaaaatctg tgattattgt tagaaaattg
    28621 taatggttta atcttcacta tcgttttcca tatctgattt ttgttttctt attgccctgc
    28681 cggataaagc aggtctgcct ccgtactgtc tatcctcaca tttactttcc tgccatcggc
    28741 ttttatctcc ccggttttac cgtttttgcc aacttcgcgg ctttggcggc ggcaatgttg
    28801 aaggcggctt cgacggtttc ggcggcattg gggttgaccc gtactcatcc gcaaacggca
    28861 acacgacgga cggtgtttgc ttttcagccg tctgccgttt tgaccaaggt gctgccgtgg
    28921 aaggagcaag tgtaggattt ttgaagaact cagttctcaa tatcctattt caaacgaagg
    28981 aaatgccgtc tgaaaattaa acacggtttc agacggcatt ttgattgttg ggttttcaac
    29041 ccgacctacg gttcctaatg attttcagat aaaaataaag aaatatcttc taacgcatct
    29101 tcagccctac cttgaagctc tggatcatct tgctttgaat gtagtaaaga aataacttta
    29161 tcgtaatcac cgatatttga atgtagcctg gcgagatgtc cgaaacaggt tagcgataat
    29221 ccggcaatat ccaagtcttt atttttcgat aattccatca atttctcaga aatccacggt
    29281 acttcatatg ctttggaatt aaataccata tcaagaatgc catctataac actttgttta
    29341 tcatcagaag aaatcaactt ttctgtttta cttttatcag tcatttttta atttttcctt
    29401 ttgaattaac taatccaatc ctgctttttt taaagcattt ttctgatcgg tatgcaaatc
    29461 ttgccaactt ctgacatgcc cgtgatattc cgcaaaaccg ttattaaaag tttgagtttt
    29521 gtctaataca acgatttcat tattggcttt atcaacccca actcttcgag gagaagttga
    29581 tttaacttga acggaattat caagagctgc ttgtccgttt gttggtgctc tacttttcac
    29641 agaattatca tttttgccat gataagaagc gtctttatat cgttggttgc ctttatcata
    29701 cggacaatca ttatgaaccc aaaccccttc cgtttccgcc tgactgccct tgacgaagta
    29761 agtatgccag tcggcaacgg tcagattgta ggcttgcagc ggttccggtt tgacggtaat
    29821 gctttgaacg gtctgctccg caccgttttc ggcaaatagc ctgcttcccg ctttcaaatc
    29881 ttccgcttta atccatttgc cgtccgaata aaacgggtgg atgcggttgg aaatcagggt
    29941 ttgactgttg tcgaggccgt ctgaaacttc aatgtaaacg gtttcttgat acggattgcc
    30001 gtatcgggcg gtaacaggtt tgtatcccgt ttctccgctt gcctcgtcct tggaaaggac
    30061 gcggtcgccg gcttgaatat gggcaatggc ttttcagccg tctgccgttt tgaccaaggt
    30121 gctgccgtgg aaggagcagg tgtaggattt ttgaagaact cagttctcaa tatcctgctt
    30181 caaatgaagg aaagaccgtc tgaaaaacaa gcttaatttc agacgacctt taatcaaatt
    30241 taagaaagcc cttaccaagc aaacggtttc cgcttcatat ccgaaaggtt gtcaacttca
    30301 ttattcagca agaactgctc aaaagcattc caacctttct tttccaccaa ttcggcttcc
    30361 tgtttataca aggggacaag caaagggaaa acgatattgt agtgttcgcc ataacagacc
    30421 tgaaaatcat cgtcgaaata aaatggggcg gaaacataca gtgcatccat ttttgttgaa
    30481 tcaacaatgc ttctgggcaa ataaacaacc tgcccccgaa gaaggatttt tttgttctca
    30541 atgatggttt ctgccaacca tcttaaaaat gggaaaatct gattttcatc atattttaaa
    30601 gaacatacga aaagtatttc aaatctgctt ttataattca gatcatgttt gcttaaccct
    30661 aaagtaatat aagtttttaa atccctgtta ggggaagagg gaatagctgc gatatttaat
    30721 ttgtactgac ttcccgaatc gcttttcgag ccatgagaaa tagtgcctat atatttttca
    30781 atatgattaa tcatattttt cttagcttcc catcactgta tcgaataatc atagaccaga
    30841 ttgagacaaa acgccttgtt cccattgata gaaagtccgt ctgaaaagta aaagtgtttc
    30901 agacggacta cgtttgccga ctatccgacc cactttatta tccaatgaga ttcttgcagg
    30961 tattctttta atatttctaa gttttcgacc cagtcatcgt aactgtcaaa tgatgagaat
    31021 ttgtcatttg aagataacag tatcagaaat ccaccggtat tttcctggtc atcaataatt
    31081 tttatgaatt tttcttcagg attgacccca ttcaggataa cccccaaagt attgaatttt
    31141 atgtccatag acacacccta atctttaatg ggaatatggt tttttcaacc caacctacac
    31201 tttttattag ttcccatacg aaccaaaacc ttcataatta caatcatttt tttgtgcaat
    31261 accaaataac gtagtttcaa tagctaaaat gttttcaagg gaagggacga tattgacgga
    31321 acaatagcaa gtccacattt ctccgaattc atcttgttca atactgattt gaaaatcttg
    31381 ccgataagaa atgagttggc tttctattga ttgcagattt ttttgagaac ctcctataaa
    31441 aaactctatt atcataggtt ttgtcaaatc aaaccctagt tcttgatatt gtttcagtac
    31501 atctgaagta gatttttcgt caacaatcat ttttaatctc catcttgttt tcggtatttt
    31561 ttccccgggc tttcaattcg attgtaatta ttggtacttt tggaaccacc gtgagcgtca
    31621 attcttctag attcatcttt gaatgcctct cgattatttt tggcaggagt aaaatctgta
    31681 gctatatgag ggtcattatt aaccctagct tctcttgctc cagagatttg ggaacgttgt
    31741 cttgtgccct ttccagaata agttcgacca ctttcatgag tattagtata agaaccaact
    31801 tctgtcctat atttttctcc atacggacaa tcattatgaa cccaaactcc ttccgtttcc
    31861 gcctgattgc ccttgacaaa gtaagtatgc caatcggcaa cggtcagatt gtaggctttg
    31921 agcggctgct gtttgagggt aatgttttga accgtctgtt ttgcaccgct ttcggaaagc
    31981 agggtgtcgc cttttttcag acgacctgcc tgtatccatt ttccttgact gtaaaacggg
    32041 tggattttat tggaaatcag ggtttggttg ttgccgatgc cgtctgaaat ttcaatgtaa
    32101 acggtttctt gatacggatt gccgtatcgg gcggtaacgg gtttgtatcc cgtttttccg
    32161 cttgcctcgt ccttggcgaa gacgcggtcg ccggttcgga tacgggcaat ggctttgtag
    32221 ccgtctgccg ttttgaccaa ggtgctgccg tggaaggagc aggtgtagga tttttgaaga
    32281 actcagttct caatatccta tttcaaatga aggaaatgcc gtctgaaaat taaacacggt
    32341 ttcagaagac attgtgattg ttggtttttt aacctacgct tgcgaaatta attcctttct
    32401 cctgaatact gcaccattta tttggtttaa ctccatagaa gaatgattag aataaataca
    32461 ttcaaagtca gcttcatcaa aatattctcc acacatagac cataactctg taatcaaata
    32521 ttccatatct tcatggtctt ctgtgatggc ttctcttgat aaataatttt tgatttttaa
    32581 aaaccttctt ccttcctcgt aatctacggt aattaacctg atatctgagt gtatcatgcc
    32641 cagcattgct atattcatac cgactcgcag ccagcttggt aatatattca taactattgc
    32701 acctttttaa tggttttagg gttagccgga acaatgtgaa ctccattttt acctgaatga
    32761 atcgtaccaa aattggtact taatcctgat ttcccatcgc tgcctatagg atacccaaaa
    32821 tcaacaacag gatttcctct tgctccttta gaaataactg gataagcacc tgaatgtatt
    32881 ccgttcaaca attcttgagg attaatgttt tgatttaaag tactcttacc ttcaatgtag
    32941 tttccatgtc ctgaaatatg tttcccttga gctccatcat gaattttagt cccaatagat
    33001 tttgctttaa aatcaacatt gcgggtttga aacatttctc catctacaga ctgagataat
    33061 cttgaagcag tgttatgctt gtaggagtct gagaaatccc cgctaaccgc agccttcccc
    33121 ggttttgccg cctttgtcag gtttttgact ttggctgctg cggcaacgtt gaagacggct
    33181 tcgacggttt cggcggcatt ggggttttcc tgtatccacc ggtcaacggc ttcgcgcgta
    33241 ttcttttcaa agcccgccac gctgcccaag ccgccgatga cggcgaattt gccctcggcg
    33301 gg
//
LOCUS       DAWFKN010000012        33093 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFKN010000012 DAWFKN010000000
VERSION     DAWFKN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.012"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(98..844)
                     /locus_tag="WGR46_000587"
     CDS             complement(98..844)
                     /locus_tag="WGR46_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0855586.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1023..1694)
                     /locus_tag="WGR46_000588"
     CDS             complement(1023..1694)
                     /locus_tag="WGR46_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0855587.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGR46_000589"
     CDS             complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGR46_000589"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0855588.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2511..2993)
                     /locus_tag="WGR46_000590"
     CDS             complement(2511..2993)
                     /locus_tag="WGR46_000590"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0855589.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3025..3714)
                     /locus_tag="WGR46_000591"
     CDS             complement(3025..3714)
                     /locus_tag="WGR46_000591"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0855590.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGR46_000592"
     CDS             complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGR46_000592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0855591.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4601..5911)
                     /locus_tag="WGR46_000593"
     CDS             complement(4601..5911)
                     /locus_tag="WGR46_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0855592.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGR46_000594"
     CDS             complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGR46_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0855593.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6086..6385)
                     /locus_tag="WGR46_000595"
     CDS             complement(6086..6385)
                     /locus_tag="WGR46_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855594.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6736..7932
                     /locus_tag="WGR46_000596"
     CDS             6736..7932
                     /locus_tag="WGR46_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0855595.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8568..10409)
                     /locus_tag="WGR46_000597"
     CDS             complement(8568..10409)
                     /locus_tag="WGR46_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0855596.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10500..10970
                     /locus_tag="WGR46_000598"
     CDS             10500..10970
                     /locus_tag="WGR46_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0855597.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGR46_000599"
     CDS             complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGR46_000599"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0855598.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11940..12422)
                     /locus_tag="WGR46_000600"
     CDS             complement(11940..12422)
                     /locus_tag="WGR46_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0855599.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHYHH
                     "
     gene            12895..13101
                     /locus_tag="WGR46_000601"
     CDS             12895..13101
                     /locus_tag="WGR46_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855600.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAAATEAPAAEAPAAEAAK"
     gene            13211..14578
                     /locus_tag="WGR46_000602"
     CDS             13211..14578
                     /locus_tag="WGR46_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0855601.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14759..16738)
                     /locus_tag="WGR46_000603"
     CDS             complement(14759..16738)
                     /locus_tag="WGR46_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0855602.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGNSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLAGWEEHLYSGLDPFHYITVTDNSHVIGQTISLDQ
                     FILENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDPKEAKN
                     TALIFEKNVTGTSNIIFENPIDDLKSLDGHQIIKVNGTADKRAFRLSGKHQKGIYTLS
                     LQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDKNSDIFDRTLPRKGLWLRLI
                     SGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLSVGLMGGQAEQRSTFRNPDT
                     DNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSKGI
                     TASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGKFSDSENAHVNLLGSRQL
                     QTRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEMDGERRVINNKTAIESQLGV
                     AVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17157..17603)
                     /gene="smpB"
                     /locus_tag="WGR46_000604"
     CDS             complement(17157..17603)
                     /gene="smpB"
                     /locus_tag="WGR46_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0855603.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17658..18668)
                     /gene="waaF"
                     /locus_tag="WGR46_000605"
     CDS             complement(17658..18668)
                     /gene="waaF"
                     /locus_tag="WGR46_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0855604.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18799..18874)
                     /locus_tag="WGR46_000606"
     tRNA            complement(18799..18874)
                     /locus_tag="WGR46_000606"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18839..18841),aa:Asn,seq:gtt)
     gene            complement(19065..19817)
                     /locus_tag="WGR46_000607"
     CDS             complement(19065..19817)
                     /locus_tag="WGR46_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0855605.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20128..20652
                     /locus_tag="WGR46_000608"
     CDS             20128..20652
                     /locus_tag="WGR46_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0855606.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQPAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(20741..21020)
                     /locus_tag="WGR46_000609"
                     /pseudo
     CDS             complement(20741..21020)
                     /locus_tag="WGR46_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(21346..21507)
                     /locus_tag="WGR46_000610"
     CDS             complement(21346..21507)
                     /locus_tag="WGR46_000610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855607.1"
                     /translation="MPSEKKMGCRIWMSDHLIRPNVGLRYSGLNSNQYGVASPCRTIC
                     TVCGFVALS"
     gene            complement(21497..22642)
                     /gene="dapE"
                     /locus_tag="WGR46_000611"
     CDS             complement(21497..22642)
                     /gene="dapE"
                     /locus_tag="WGR46_000611"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0855608.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22755..23375)
                     /locus_tag="WGR46_000612"
     CDS             complement(22755..23375)
                     /locus_tag="WGR46_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0855609.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23409..23918)
                     /locus_tag="WGR46_000613"
     CDS             complement(23409..23918)
                     /locus_tag="WGR46_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0855610.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(24013..24564)
                     /gene="azu"
                     /locus_tag="WGR46_000614"
     CDS             complement(24013..24564)
                     /gene="azu"
                     /locus_tag="WGR46_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0855611.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(24845..25267)
                     /locus_tag="WGR46_000615"
     CDS             complement(24845..25267)
                     /locus_tag="WGR46_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0855612.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25398..28148
                     /gene="secA"
                     /locus_tag="WGR46_000616"
     CDS             25398..28148
                     /gene="secA"
                     /locus_tag="WGR46_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0855613.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTNQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28163..28315
                     /locus_tag="WGR46_000617"
     CDS             28163..28315
                     /locus_tag="WGR46_000617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855614.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            28293..30065
                     /locus_tag="WGR46_000618"
     CDS             28293..30065
                     /locus_tag="WGR46_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0855615.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKKQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30252..32180
                     /gene="rpoD"
                     /locus_tag="WGR46_000619"
     CDS             30252..32180
                     /gene="rpoD"
                     /locus_tag="WGR46_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0855616.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            32327..33093
                     /locus_tag="WGR46_000620"
                     /pseudo
     CDS             32327..33093
                     /locus_tag="WGR46_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         8440 a         9355 c         7758 g         7540 t
ORIGIN      
        1 caacgccgta ccggtttttg ttaatccacg ataaatttgc cacaaaaaag ctgcctcaaa
       61 tgaatacccg ggcagctttt tgttgatatg actccaatca gcggtgttgc ggattgtaac
      121 gtttttccaa acgcaggaat atccagccta agaaagtcgt catcaacaga taaatcaggg
      181 cgacggtgta aagcggttct tcataaaccg aataccggcc cgtaatcgta ttctgaacat
      241 acgccaactc cgccacagca atgaccgaca gcagcgagct gtctttcaag agcgtgatga
      301 actcgctcgc caaaggcggc agcatgcggc gcaatgcctg cggcagaatc acatagcgca
      361 tcgcctgcgg ataggtcagc cccaaagaac gcgccgcctc catctgtcct ttgtctatag
      421 actggatgcc cgcgcggaaa atctcacaga tatacgcccc cgagttggcg atcagtgcca
      481 aagaaccggc aatcagcggc ccgtatccgc gacgcagcgc gattgccgcc tcgccgctga
      541 ccaaaatgcc gtctgaagga tggacgaaaa acggaaacca cacatacgcc caaatcacaa
      601 tctgcacaaa cagcggcgta ccccggaaca gcgtaacata cagcagcgaa actttacgca
      661 acgcccacgc cagcacgcgc atcggcgcac cggctttttc caagtgaatc aggcgcgcca
      721 acgccaacaa cagacccaat accgaaccgc ccgccgtcgc cacgaccgtc agccccaagg
      781 tcgtcagtgc gccgtaaagg aacatccagc ggtattcgta aataatgtca aaacgaaaat
      841 ccatataccg tccgtatcaa aaaccggcgg aactgccgcc gttgcaaaat aatccgccat
      901 tttaccgtaa aaaccgccgc ctgaactttt ttatcgcggc agacggcggt tgcgcgtctc
      961 cgcaaaaatg cagggcgcgc ggttttcaga cggcatttgc cgttcaaagc cgtgcgctgt
     1021 ctttaccaaa tgcccaacca ttcgcccacg gcatccatcc aatccttatt gcccccgccg
     1081 ctcctgcctg cccggcggta cgcccacggc gcttgcggat ttttagcttt ccacaatcct
     1141 ttgcgttccc tttccgcctg aatttgagcg tcggcataat cggcaaaatc cgccttatcc
     1201 tgctgttctt tagcataact tttataatgc cacgccgccc cgtcctgcac ctgcatcagg
     1261 ttcaaatcgg ttttgccgac agaaacctgc gccacttcgc gctggtagcg gtcggtgtcg
     1321 aacacgcgca cgctgacttt cctgccttcc gccgccgcac gcaggttgtc gcgcgaacgc
     1381 gtgccgtaag cctgtttcat ctccggcgcg tcgatatacg ccatccggat tttgtgtttc
     1441 gcgccgtcgc cgtcgataac gtgaagggtg tcgccgtcat agactttgga caccgtgcct
     1501 gtgtagcggt ggccggattt cgccgatgct cggcggcggg cgggcgcgtc ggaacccgcg
     1561 tcccctgccg cgccgagtac gtcgagtacg gcaaccgccg tccgcaccgc ctcgctgccg
     1621 taccccgtat aacccaacgc acccaaaagc gacaaggcga cgggaagcca tttcatgatt
     1681 tttttaatct gcatattttt caaatgccga tgccgtctga acatatcgga atcggatttc
     1741 agacggcatc ttaacgtcag gattaccctt ggcagggata gatgactttc gcaccctctt
     1801 ccgtccccaa aatcaacaca tcggcggcat cgcgggcgaa tatgccgttt tcgagcacgc
     1861 cggtgatttt gttgatttcg tcttccatcg tcagcggctg atcgatattc aagccgtgga
     1921 catcgacgat ttggttgccg taaaacgtgg tgtagccgat acgcagttcg ggctgtccgc
     1981 ccatagcgag cagtttgcgc gaaacaagag agcgcgcgct ttcgacgact tccacaggca
     2041 gagggaattt gcccaaacgt gaaacatatt tgctttcatc cgcaatgcag acgaattttt
     2101 cggatgcgct ggcgacgatt ttttcgttga ggtgtgcgcc gccgccgcct ttaatcattt
     2161 gcagggcgtg gttcacttca tccgcaccgt caatatagac cgccaacccc gatacttcgt
     2221 tcagggaaac gacgggaata tcatattggg caagcagttc actggatttt ttggaagtag
     2281 ataccgcgcc tttgattttt ttgccgctct tacccaaggc ttcgatgaaa aagttgatgg
     2341 tcgaaccggt accgatgccg atgtattcgt tttcgggtac gaattcgact gctttttcgg
     2401 cggcgatgcg cttgagttcg tcttgtgtcg tcatattttt gtcctttggg aaaccgtatc
     2461 aacaaacagc cgccatctta acattttttt gcacgtcctg cccgccgcgt tcaaatcctt
     2521 accagcaata ccgccgcctg cgcctctatg ccttccatgc gcccgagata accgagtttt
     2581 tcgttggttt tgcctttgat gttgacgcac gaaatgtcta tgcccaaatc ggcggcgatg
     2641 ttggcacgca tttgcggaat atgcggcgcg agtttgggtt tctgtgcaat cacggtcgta
     2701 tcgacattga ccgcctgcca accctgcgcc tgaacgcttt gatacgccgc acgcaaaagg
     2761 acgcggctgt ccgcatcttt gaactctgcg gcggtgtcgg ggaaatggct gccgatatcg
     2821 cccaaacctg ccgcaccgag cagcgcgtcg gtaacagcgt gcagcagcgc atcggcatcg
     2881 gagtgtccga gcagcccttt ttcaaatggg atttcaactc cgccaagtat cagctttctg
     2941 ccttcggtca gttggtggac atcgtagccc tgtccgatac ggatgttcgt catcgtttgt
     3001 gttcctgatg ttttgaattg aagttcagac ggcattgagc agcagcctga cgatgtatgc
     3061 gtcctgcggc tgcgtcagtt tcaaattgcg cgcgtcgccc tgcaccagca aagggcggat
     3121 gcccaatttt tccacggcgg acgcttcatc ggtaatgccg tccaagtttt ccgcagccaa
     3181 tgcgcggtgc agcagccccg cgcggaaaag ctgcggcgtt tgtgcctgcc aaaggctcgt
     3241 ccgctcgaca gtggcactga tttgtccgcc ttctgcgcgt ttgagcgtat cggcaatgga
     3301 aattgccaaa atcccgcctt cggcggcgtt gcccgcctgt tctatcaacc gcgtcaaagc
     3361 ttcagacggc aggcagcagc gcgcggcatc gtgtaccaga atattgtcat ccgccgatgc
     3421 caagccgatt tccaacagtt ttgccacacc gtttcggacg gtttcggcgc gggtctgtcc
     3481 gccgtttttc cacacccgaa cctgtggaaa tgccgtctga accttatcgg caaacgtgtc
     3541 ttcgggcgag acgacaacga cggtcaaatc gacggcctca tgccgttcaa aaatcccaat
     3601 cgtatgttct aaaacggttt tgcttccgat ttcgacatat tgcttgggtt tgtccgcacc
     3661 gaaacgcgcc ccgatgccgg cggcgggaat cagcgcgata tttttgcgct tcatgcgtcc
     3721 gtcccgccgt tttcagacgg cacggcttcc ttgcgccaga tacaggcttc gcccaagccg
     3781 tccaaatatt gcccgtgcgc cgccaactcg ttttcgtccg ccctgatgac tttcagtttg
     3841 ccgctgcgtt tggtttcggt atgcaccacg ggtttggttt ccattttttc ctctgcggcc
     3901 gcacccatca ggtcgaactg ccgccgcgtc atagcaagat agacttcgcc caaaagttcg
     3961 cagtcgatca atgcgccgtg caggacgcgc ttgctgcggt cgacggaaaa acggttgcac
     4021 aaggcatcca ggctggcttt ctgcccgggg aacatttcgc gcgccatcgc cagggtatcg
     4081 gtaacggtac agccgagttc ctcaacggtc ggcaacccca tccggcggaa ctccatattg
     4141 aggaagccca cgtcgaattt ggcattgtgg ataatcagtt ccgcaccgcg caggaaatcg
     4201 gcaatctgcc tgccgacctc tgcaaacggc ggcgcgtttt tcccttccaa aacctgtatc
     4261 gtcaagccgt ggacgcgtgc cgcctcttcg ggcatatcgc gctcggggtg gacatagagg
     4321 tgcaggtttt tgtcggtcat ttggcggttg accatttcca aaccggcaaa ctcgaccaag
     4381 cggtcgccgc cgtcggcata cagaccggtg gtttcggtat cgaggatgat ttggcgtgtc
     4441 gtcatatcgg tgtctttctt ctatcttcgt aaattgctta ttttttaagc aatgtatttt
     4501 tctgttttca tttcaatgca caaacccact tattcacagt gtgttcacaa cattgggcag
     4561 gcggattgtg tattttgggg acaatttttt cagacggcat tcaaggtttt ttcctgattg
     4621 ccgccgcgcc taaaaaccgc ctttcgcgct taatcaaaaa taccgacaac ggaatattgc
     4681 ccaaagcgac aatcagatac aacaaggaaa tgctgtcaaa caaaaacagc aacaccgcgc
     4741 tcaaaacggc agcggaaacc ataaaaatac cgttaacgat attgttggcg gcaacggcgc
     4801 gggcgcggaa agtctcgcta ctggcggttt gcagccaggt atagagcgga acggagaaaa
     4861 atccgccgaa aaagccgatc agcgtcatca ccgccatcac gggatatgcc catccttgcg
     4921 ataaaaacca aaaaatgccg ttcagccctt caaaacggtg tccgtgcgtc agccacacca
     4981 aaaccaggcc gcaaaccgtc aaacccaacg caccaaccgt tacccaagcc aacatcaggc
     5041 gttccctgct gaacttggca cacagtaccg aaccggcggc aataccgatg gaaaacagag
     5101 caagcatcag gttgaaaaca ttgtcgttgc cgcccagatg gatttgggta aaggtcggca
     5161 gttgcgtggt ataaaccgcg ccgacaaacc aaaaccacga aataccgata atggcggtaa
     5221 aaacgggctt gtgccgcacc gtttcacgca gcagggattt tgtgccacgg acaatattcc
     5281 actcaatttg tgtatcggca gccttggcgg gtacggacgg cataaacagg ctgccgaccg
     5341 tgcctccgac ggcgaccagc aaaaccagta tcccgacaat ataaggcggt acacctgcca
     5401 ccgccgttcc caaaatctga ccgaacagga tggcgacaaa cgtacccgat tcaatcaggc
     5461 tgttgcccat catcaactct ttgtcgtcga gataatcggg caggatggcg tatttcagcg
     5521 gcccgaacag cgtcgattgc gcgcccatgc aaaacagaca cgccaaaagc agcggggcag
     5581 accggatata aaacccgtat gccgccaccg ccataatgat catttccagc accttgaccc
     5641 aacgcgccaa aacggccttg tcgaatttgt tacccaactg ccccgacagc gaggaaaaca
     5701 ggaaatacgg caaaataaac agcaacgcgc ccaagttcaa catctgtccg gcaggcagga
     5761 agccgttttg ccccaaaccg taaaacccaa tcatcacaaa cagcgcggtt ttgaacacat
     5821 tgtcgttgaa cgcgccgaga aactgcgtag cgaaaagagg tgcgaaacgg cggcttttaa
     5881 ccagtcccaa accgcctttt ttagcgtaca tcgttttccc tctctttttc aatcagttta
     5941 cttgtcgaat catcatccat caggatgcgg tgcgccggcc cttccaagtc gtcaaactgc
     6001 ccgtttttgc ccgaccacca aaaaaaccag ccgatgacaa acgccaaaat aatgctgatg
     6061 ggcaccaata taaacatgct ttccatcaca tattccctgt caaatcgttc aaaacaaaag
     6121 tctgccccga cacggtcaga tattcgttac gcaaagttcc gacgggagct tcgtcaaaaa
     6181 acagctcgat acggtctttg accacgcgcc aatattgggg gatttccgtc tgaccgaacg
     6241 gcgacaggac atgattttcc attccgcctt caagtttgac ggcaaaacgc ccgctttgcg
     6301 gccgtgcttc cgattcgtcg tcggcaagca ggatgaaaaa gcctatatgc cgtcccgatt
     6361 ggtcatgaat actgaaataa tgcataaatt tcccacccgc cttttttcag acgacaccaa
     6421 ctaaaaacag ggcgaatgta ccagtttgga cgggaagaat gcaaagaaat tctccctccc
     6481 ccagccgaaa acaccggcaa accgcatatc cccctttttt ccgtcaaaat gcccgacttc
     6541 cgccattttc acgcaaacgc ccgattaagc caagcaattg caaagatttt ttgctagaat
     6601 agcctgcttc ttttatcaac cttttcagac ggccccacta ctttcccgcc caggaaggca
     6661 aaacggattc ggcacgaatc cggttagtat ccgtgtccga ttccaatgcc gtctgaaact
     6721 ttccggagta agaaaatgtc ccaaaaattg atcttggttt tgaactgcgg cagctcgtcc
     6781 ctcaaaggcg cggtcctgga taacggcagc ggcgaagtcc tgctcagctg ccttgccgaa
     6841 aaactcaacc tgcccgatgc ctacatcaca ttcaaagtaa acggcgaaaa acacaaagtc
     6901 gatctgtccg cacatcccga ccacaccggc gcggtcgaag ccctgatgga agaactcaaa
     6961 gcccacggcc tcgacagccg catcggcgcc atcggccacc gcgtcgtcag cggcggcgaa
     7021 ctgtacagcg aatccatcct cgttgacgac gaagtcattg ccggcatcga aaaatgcatc
     7081 ccgctcgccc ccctgcacaa ccccgcccac ctcttgggcc tgcgtgccgc gcaaagcatt
     7141 ttcaaaggcc tgcccaacgt cgtcgtattc gatacctcct tccaccaaac catgcccgaa
     7201 gtcgcctaca aatacgccgt tccgcaggag ttgtatgaaa aatacggcct gcgccgttac
     7261 ggcgcgcacg gtaccagcta ccgcttcgtc gccgacgaaa ccgcgcgctt cctcggcaaa
     7321 gacaaaaaag acctgcgtat ggtcattgcc cacttgggca acggcgcgtc catcaccgcc
     7381 gtcgccaacg gcgaatcgcg cgacaccagt atgggcctga ccccgctgga agggctggta
     7441 atgggtacgc gcagcggcga catcgatcct tccgtattcg gcttcctcgc cgaaaacgcc
     7501 aatatgacca tcgcccaaat cactgaaatg ctgaataaaa aatccggtct gctcggcatt
     7561 tccggcctgt ccaacgactg ccgcaccatt gaagaagaag ccgccaaggg gcataaaggc
     7621 gcgaaattgg ccttggatat gtttatctac cgccttgcca aatacatcgg cagtatggcg
     7681 gttgccgcag gcggtttgga cgcactggtc tttaccggcg gcatcggcga aaactccgac
     7741 atcatccgcg aacgcgtgat cggctacttg ggcttcctcg gtctgaacat cgaccaagaa
     7801 gccaacctga aggcacgctt tggcaacgcc ggcgtgatta ccactgccgg cagcaaagcc
     7861 gttgccgtgg tcattccgac caacgaggaa ttgatgattg cacacgatac cgcccgcttg
     7921 agcggtctgt aagattcaat ccccacgcga actgcctccg aaaacggagg cagttttttt
     7981 atccggcttt ccatgcttaa acagcaccgc ctcttttcag acgttgacgg ttgcagccgc
     8041 ttacctgaac cttatagtgg attaacaaaa accagtacag cgttgcctcg ccttagctca
     8101 aagagaacga ttctctaagg tgctcaagca ccaagtaaat cggttccgta ctatttgtac
     8161 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatcc gactttccat
     8221 acttaaacag cactgcctct tttcagacat tgacggttgc agccgcccac ctgaacctta
     8281 atgattgact attttttaat catgttatta ttttccataa aatacatgac attaagatgt
     8341 ttttccacaa aagatacaca caccggcaaa caccggctgt gtttatcttt tcttatgcct
     8401 attttttaat catcgtattt ttatctttta atttcaatac gcaaactaac ttatacacac
     8461 ggttttcaca tctttggact gcttccgtgt gtatagcgga tattgccgtt ttcctttctg
     8521 acaaaaatgc cgtctgagaa cttcagacgg catttgaaac atcggaatca gcggttttgt
     8581 tcataccact cgataaactt gtctgctttg acaaaaccca gcagcggctc gctgcggctg
     8641 ccgtcggagc ggacgacaaa cacgcccggc ggcccgaaca gaccgtattc tttcaacaac
     8701 gcctgatgtt cgggcgtgtt ggcggttacg tcgatttgga aaaagcgttc catatcgact
     8761 gcctgatgca cttccggctg attgagcgtg taagccgcca tttctttgca ggaaatgcac
     8821 cagtcggcat aaaaatccaa aacgacgggt ttgtcgggat gttctttcaa cgccgtatcc
     8881 atcgctgcct tcagcgcggc agtatcggca aacattttgc cgtgttccga agatttgcct
     8941 gcttcggccg gcggattgag ggtcaggaaa tggtgcagcg cggtcgtttt gccgtttgcg
     9001 ccctgccagc cgaaccacgc gccgcctatc agcaatatgc tgcccaatgc gaatgccaca
     9061 gctttcggac ggcgtttctg cctgcgtccg ttgaccagca gcatacaggc aggaaccagc
     9121 atcagcagcg tgtacagcgc gacgacgaga taatagggca agtgcggcgt ggcgaggtaa
     9181 acggcgacgg caagcaggat aaagccgaaa gcgtatttga cggcattcat ccaatcgcct
     9241 gccttaggca ggatatgccc gccgaacgtg ccgatggcaa tcagcggaac gccggtgccc
     9301 aacgccaaag tgtaaagtgc caaaccgcct aaaaccgcat cgcccgtctg accgatgtag
     9361 cccaaagcaa atgccagcgg cggggcgacg cacggcccga caatcagcgc ggacaatatg
     9421 cccataataa agacggaaac gattttaccg cctgaaagcc tgctgctttg attctgaaaa
     9481 tacgactgca cggcgttggg aagctggatg ttgaacagcc cgaacataga cagtgccaag
     9541 acgaccatca aagccgatgc cgccaacacg acccaagcct gctgcaacca tacggtcagc
     9601 agtgcgcccg tcagtccggc aacaatgccg accagcgtat aagtcagagc caaaccctga
     9661 acataaacga cggacagcac aaacgcccgc gccttgcccg cctttttgtc gccgaccaca
     9721 atactggaaa caatcggcaa caggggatac atacaggcgg taaaactcag acccaaaccg
     9781 gcgagaaaaa acgccaaaag attggcgttg agcgtatccc aagacagctt gaaacggctg
     9841 tcgccgccct catccccctt cgggggcggc agcgccccgc tgccgttttg agaggaaggc
     9901 tgcaaaaagc ggtctttggc ggatgccggt tcgtcggttt gcggatggta agtgccgttg
     9961 ccggaaatat caaactcggt atccacgggc ggatagcaca cgccggcttc ggcacagccc
    10021 tgataggtca aaaccaattt atacggttcg ccgacagcct ttgcataagg aaaggcaacc
    10081 tgcgcttcgt gatggtaaac cgtctgcctg ccgaaaaact cgtcttcctt ctcttcgccc
    10141 ttgctgaaag aaggctgtcc caacaaatcc gccggatcgg tcttgccgac gattttcgcc
    10201 tgatacatat agtatccgtc ggcaatcttg aaacggacgt tcacaccgtc gtcggcaacg
    10261 gcaagctccg gcacgaatgc cttttccggc ggcagcagat cgttcgcatc cagcgcgaaa
    10321 gctcgtccgc acaacatcaa aaatacggcg aacaggcaaa tcagtttttt cataatcgaa
    10381 tccgtttcag acaaataatt tgtctgcatt ataaatggta aggttgacgg tgggatttaa
    10441 tttatgtaaa acccgccatt atccgaacct atttccataa ccatcttatc gaacccgcca
    10501 tgtacgatgt caatacccac gatgtccgcc gctttttcgc ccgcgtgtgg cagcagcggc
    10561 tcaatccgct gcaactgagc gcactggaac agaaagccct ccgcattgtc gaagcccatc
    10621 ccgaatacca ccgctatctc gaacgcatcg aagaccatct ggacaccgac tggctgcccg
    10681 aaaacggcga aagcaacccc ttcctgcata tgtcgctgca tctgtccgtc caagaacagg
    10741 cgggcataga ccagccgcac ggcatacgcg caatccacga caccctgtgc gccaaacgcg
    10801 gctggctgga agccgaacac gaaatgatgg aggcactggc ggaaacactg tggacggcgc
    10861 aacgctacgg caccggtttg gatgtcaatt tctacatgac ccgactgcgc aaactcatcg
    10921 gcttgggtgc agaagatcaa gccaggttga acccacatga aatcgcctga ccataccaac
    10981 cgcctgcaaa atgccgtctg aagcggaaca acccctttca gacggcatgc atttccccca
    11041 tcatttccac aacgcgcgtt tcagcataat caaccaatcc ttcttatcca aaacggggcg
    11101 ttgtgcaaac acatcgtatc ggcacgcgtc cagtttctgc aaaatcaact gcgcccccaa
    11161 cacaatcata cggagttcca aaccgatacg ccctttcaat tcgcgcgcta aaggcgaacc
    11221 cgccttcagc atacgaaatg cgcgccggca ctcatacgcc atcagccgct gaaacgccgc
    11281 atccgcccta ccctccgcaa tctgtccttc ggaaacaccg aatttcaaca aatcgtcctg
    11341 cggaatataa accctgccct tttgccaatc cacagccaca tcctgccaaa aattcaccag
    11401 ttgcaaagcc gtacagatgc cgtcgctttg cgccacgcac accgcatccg ttttcccgta
    11461 caaagccagc ataatgcgtc cgacaggatt ggcagaacgt cggcaataat cggccagctc
    11521 gccgaaattt ccataccttg ttttaaccac atcctgagaa aatgcagaaa gcaaatcata
    11581 aaacggctgc aaatccaaac cgaacggcac aaccgcctcg gcatccaatc gtgcaatcaa
    11641 aggatgcgcc gaccggccgc ccgatgccaa cacgtccaac tcgcgccgca aaccctccaa
    11701 ccccgccaac ctggcttcag acggcatact gccctcgtcc gccatatcgt ccgccgtccg
    11761 tgcaaacgca tacaccgcgt gaaccggctt cctcaaacgg cgcggcaaaa tcagcgaacc
    11821 gacgggaaaa ttctcataat gcccaaccga cataccttct ccatccatca aacaaaatgc
    11881 cgtctgaaac ggaacaaacc cttttcagac ggcatcagat acctccaagc tgccggcaat
    11941 cagtggtggt aatgaccgtg cgggccgtgg acatgaccgt gtgcgatttc ctcatcggat
    12001 gcatcgcgca cgctttcaac cgtagcctta aagcggattt tcatgcctgc caaaggatgg
    12061 ttgccgtcca ccaccgcctt gccgtcggca acatcggtta cacgatagac gacaacatcg
    12121 ccggtttcag gatcgtcggc ttcaaacatc atgccgactt cgacttcaac agggaacacg
    12181 cccgcatctt cgatacggac caactccgga tcctgctcgc cgaacgcatc gtcgggcgac
    12241 agcgccacat cgaccgtatc gccggcatcc ttaccgtgca acgcctcttc caccaaaggg
    12301 aaaatgccgt cgtaaccgcc gtgcagatac gcaatcggtt cttcggtttt gtccaaaagc
    12361 tgattgttgg catcatacat ctcataatgc agcgaaacca cggaattttt cacgatagcc
    12421 atatttgtcc tttcaggaac agcagattga ttacaggcgc attctaacac aaccgccgcg
    12481 ccggccgatt accgttaacc tgttcataaa ctgtacagca catatttcaa tgtaaatctt
    12541 tgttatttta ttgcggtgta acttttttac aacattctta aaaccattcc gacctgtctg
    12601 ccgactttcc caatccgcct taataaatca tacaagatac tgaaattata ttaatctcta
    12661 taatatttat ccctatcgaa tttttaacag caaaaccgtt ttacaggatt tatcaatccg
    12721 cccgccagaa aacttttcat tcaaaccttt ttcccatctg tacgacattg caatccctta
    12781 ttccatagtg cataattacg caaattcagc gatgaatttc caacctggtt tgtagtatgg
    12841 tcgataaaga cctatttgtt tcaataattt aaattggttc taaaggttac taaaatgaaa
    12901 aaatccctgt ttgccgctgc tttgttgtct ttggttctgg cagcctgcgg cggtgaaaaa
    12961 gccgctgaag ctcccgctgc tgaagcacct gccgccgaag ctcctgctac tgaagcacct
    13021 gccgccgaag ctcccgctgc tgaagctgcc gctaccgaag cacctgccgc tgaagctcct
    13081 gctgccgaag ctgcaaaata agcattttcc gcttgcaaaa aagcaggata cgttcagtat
    13141 cctgcttttt tgatttttca gacggcatca gattcccttc ctcaatcttc tccctaccct
    13201 tccgacaaac atgcctgacc ttcataccga attttcccga ctcctgccgg cagatgaaat
    13261 tgccgaactt tctccgacgc ttttaaaaga ccagcgcaac cgctttacgt ctgcaccaga
    13321 catcattttg cagccgcgca gcgttgaaag cgtgcaaacc attatgcgtt tttgttacaa
    13381 acaccgtatt ccggttacgc cgcaaggcgg caataccggt ttgtgcggcg cggcagtatc
    13441 ggaaaacggc gtattgctga acctttccaa actcaaccgc atccgcagca tcaatttgtc
    13501 agacaactgc ataaccgtcg aagcaggttc cgtactccaa accgtccaac aggcagccga
    13561 agcctcaaac aggctgttcc cactcagtct cgccagcgaa ggctcgtgcc aaatcggcgg
    13621 caacatcgcc tgcaatgccg gaggtttgaa cgtattgcgt tacggcacga tgcgcgacct
    13681 ggttatcggt ttggaagtcg tcctccccaa cggcgaactg gtttcccatc tccatcccct
    13741 gcataaaaac accaccggct acgacctgcg ccatctgttt atcggcagcg aaggcacact
    13801 gggcatcatc actgccgcca cactcaagct ttttgcccgt ccctcggaca aggcaacggc
    13861 atgggtcggc atacccgaca tcgaatccgc cgtccgcctg ctgaccgaaa cccaagctca
    13921 ctttgccgaa cgcctatgca gttttgagct gatcggccgt tttgccgccg agttgtcttc
    13981 cgaattcagc aaattccccc tgccgacaca ttcagaatgg catattttac ttgagttgac
    14041 cgactcatta cccgacagca atcttgatga tcggcttgtc gaatttcttt ataaaaaagg
    14101 atttaccgac agcgtattgg cacaaagcga acaagaacgt atccatatgt gggcattgcg
    14161 cgaaaacatc tccgcctccc aacgcaaact gggcaccagc atcaaacacg atattgccat
    14221 tcctatcgga cgcgttgccg actttgtccg ccggtgcgcc aaagatttgg aacagaattt
    14281 taaaggcata caaatcgtct gcttcggaca tcttggcgac ggcagcctgc actacaatac
    14341 tttcctgccc gaaatcctca gcaatgaagt ctatcgttac gaaaacgaca tcaacgacgc
    14401 agtttatcgc aacgttcttg cctgcaacgg cacgattgcc gccgaacacg gcataggtat
    14461 catcaaaaaa cagtggctgg acaaagtacg cacgcctgcc gaaatcgccc tgatgaaaag
    14521 catcaaacaa caccttgatc catataacat tatgaatccg ggcaaactgc ttccgtaacc
    14581 ggcatttctg atttgcatac acaacaaaga aagggacaat agatccgatt gtcggtttag
    14641 cgcgagctcg tgagtgcggt aaaaaattgg tggaaatgac acgaaaaatg accgcatttt
    14701 taaaataaaa aaatcggcag tgaatttctc tgccgatttt attttgttac aacttaactt
    14761 aaaacgtcca ctgtaaattc aatgcgcctt gtttagcttg atgatgtttg cctgtttggc
    14821 gattgaatgt ggcttgtaaa gttaagtgag atttgatttt cactgctaca cctaattggc
    14881 tttcaattgc cgtcttattg tttatcactc gacgttctcc gtccatttct accccgaaag
    14941 gtttttgttg gtagattgaa ttgacagcaa caaatggttg gaaagtgacg ccattagtga
    15001 aagcaaattg cgctttagct tgaacgccaa cccgagtttg taattggcgg gagccaagta
    15061 aattcacgtg ggcattttcg ctatcgctga attttccgtt tacccccaaa taagtcaatt
    15121 gcgcctgtgg ttgtaggtaa aaacgaacac ggttgccctt tttagtgaag tgttccgcca
    15181 ataaagcatt gtaacccgct tcaattgagg cggtaatacc ttttgaagta aaacgttctg
    15241 tagcatcttc agtgttaatg cggtggcgga agcgttgata ttgtacccag ctatccgcat
    15301 acgcacctgt ctgtttgtcc tgaagctgat gccaagtggc gtaaacgcct gcaccaaagc
    15361 ctttcacatt tcccgttgta agattgtctg tatctgggtt gcggaaagta ctgcgttgtt
    15421 ctgcttgtcc gcccattaag ccaacggaaa gttgatagtt gtgattttgc aatgagaaaa
    15481 catcgccacc aagttgtatg cctttacgat ttccttctac tggtgcggtt ttaccttgta
    15541 catcttggct taagtgacca ctaattaaac gcaaccacaa gcctttgcgt ggtaaagtgc
    15601 ggtcaaaaat atcgctgttt ttgtcgttca aacgtaggtt gaataaagta ttggcagctt
    15661 gagcctgttg tgcataaatc gcaatatcat cgcgttcttg cactttggta aaaaagccct
    15721 ctgggcgttg ttgtaaagag agtgtataaa ttcccttttg gtgtttgcca gaaagacgga
    15781 atgcgcgttt atctgctgtg ccatttactt tgataatttg atgcccgtcg aggcttttta
    15841 aatcgtctat tggattttcg aagatgatgt tggaagtgcc agtaacattt ttctcaaaaa
    15901 ttaatgcagt atttttcgct tctttaggat cgtaagcaaa acgaaaacga gctccgccag
    15961 cataatcttc tttgacgagt aaactttcac ttttagtatt aaaacggatg tctgcattcg
    16021 ttgtttttaa ttccccaaca tcagaatccc aacggggttc ccagagagaa ttttctaata
    16081 tgaattgatc caaactaatt gtttgtccga taacgtgtga attgtctgta accgtaatat
    16141 agtggaatgg atctaaacca gaatatagat gctcttccca accagcaagc ttattaagtg
    16201 ttgggcgaag ccattcgata taattaccta cagataaaat ttcgctgttg atatggctat
    16261 tttttatttg tggagctaag gcgaaaatat cgctctttat taatcttggg ccagagtttc
    16321 cgaaggatgg ttgttcacac tgatcattat tggaattccc agtatttaca cagtccttaa
    16381 aatattgctg gagtgttttt tcttcttccc catttctttt ttcaaccccg aaacctcggg
    16441 cgaagccaac aaggtaatct tcagtgtatt ccttgtcata aaaagaaatc ttttttgagt
    16501 tgttgatgtt ttcgaattgg tgtgttttag agtatagtgc gggaactttg agagagttct
    16561 gagagctacc attcgtcggt tttagaagat aagtttcttg tttccagtat tcgctgcttt
    16621 tatctctaag tttttttaag cgatcaattt gctcatttgt taatttagaa ttgtcgtaaa
    16681 cgtaatcaac agccaaaagc ggagaggtat aaagaataga aaaaaataga cttataataa
    16741 atgatttttt aaacttctgc ttgcttgctt gcttcgagtt tcataataaa ttttcctttg
    16801 tcaagtaaaa ataaatgggg cgtggatttt agcatagaat tgaacaaaaa atgtcattta
    16861 gctcacattt ttctctattt atttcttatt tattaaaagt aaacgtttgc tttttgctat
    16921 tttgtcaagc cagtttgaaa atgtgtataa ttgccctcgt tatttacaaa aatttcagga
    16981 aaaatgaccg cactttaccc ttggctaatg ccaatttatc atcaaattgc tcaaaccttt
    17041 gacgaaggtt tggggcatca tgccgtactg attaaagcgg atgctggttt aggtgtagaa
    17101 cgtttacaca tcaggcggca gccttgccca taccgtctga agcactgttt ccacaatcag
    17161 cgcgtatgct taatcaaccg ctgtttctcg cgtttccaat ccgcctcttt catactctga
    17221 cgtttgtcgt gctgtttctt accttttgcc aaaccgattt ccatcttgat ttttccgcgc
    17281 gaaaaatgca aatccagcgg cacgatggtg tagccggcac gttcggtttt gccgattaat
    17341 ttgttgattt ccgactggtt caacaagagc ttgcgcggac gtacggcatc tggtttaatg
    17401 tgtgtcgagg ctgtgggcaa agccgtaata tggcagccga ccagataaaa cgcgtctttt
    17461 ttccaataga tataactctc tttaagctgt acgcgcgcgg cgcggattgc tttgacttcc
    17521 cagccttcca agaccaaacc ggcttcaatc cggtcttcaa tgaaaaaatc gtgaaatgct
    17581 tttttattgt tcgcaatagc cataaacatc ctatcaatat ccgccgtcag acggcataaa
    17641 cccgaaaaca gaacccatca taccgcctct tcaaccgcct gcacaatctt ctcgggatac
    17701 agcctgttga ggcagtcggt atgccccaac ggacattcac gtttgaaaca gggcgaacat
    17761 tccaagtgta ggctgacgat tttcgccctg tcgctcaaag gcggcgtatg cgtcgggctg
    17821 gaagaaccgt aaaccgccac caccttcctg cccaatgccg ccgccaagtg catcagcccg
    17881 ctgtcgttgc acacgaccgt gtccgccaac gacagcaaat ccattgcctg cgacaaatcg
    17941 gttttgccgc acaaattgac acacataccg tctgaaaggc agttgatttc ctcggcaatt
    18001 tcatcatctt tttgcgaacc gaacagccaa acctgccaac ccgccgccga ataatgtttg
    18061 cccaactcgg caaaatacct tgtcggccaa cgctttgccg gcccgaattc cgcacccgga
    18121 caaaaagcca gaacaggctt tccaatatcc aagccaaagg tttcgacaga aatttcccgc
    18181 cgccgttcat caatggaaaa ctcggggaat cccgaatgcc cgtcaaaatc ctcctggctc
    18241 ggatgcgcga gagccgtata tctgtccacc atcaaaggca gacgttcctt atccagcctg
    18301 cgtatatcgt tcaacagaaa ataacggctt tcaccgacat aacccgtcct tttaccgata
    18361 cctgtcgcca gcgcgatgat tgccgatttc aaagaaccgg gcaacacgat aacctgatcg
    18421 tatccgcgcc gccccaaatc cctaccgacc cgccaacggc gtttcaactc caacgcaccg
    18481 tgtccgaacg gattttcaag aatctcattc acttccggca tacgctcaaa caccgccatc
    18541 gaccacttcg gtgcgaacac atcaatcgtg caaccggggt gaagttcctt caaacggcgg
    18601 aacaagggct gggtcatcac gcagtcgcct atccaactgg gggaaataat caggattttg
    18661 atggacataa caagaaaccg aaatcagaca ggcggaattt taccgcgaaa ccgttggaaa
    18721 acctatcttg tctcattccg aacgccgtac gtgcaaatat gaaaaagccc gaacattcaa
    18781 gttcgggctt caaaattctg gctccccgac ctgggctcga accagggacc tgcggattaa
    18841 cagtccgtcg ctctaccgac tgagctatcg gggaatgggg cgtattatag cgtccggaaa
    18901 aaatgtgtca atccttaatt ttggaaaaat gggcgacaaa acgacaagca tatgaatcag
    18961 aaagacatta agaccgatgc cttaaaagga ttgccgttgt atgaatttcc acagccgtca
    19021 tcacaccata tttaagcccg atgagccgtt ctgccctccc ccgcttaaaa caatgccgtc
    19081 tgaacttcgc cgcgttccaa agccagtaaa aactgtttgc ggttcaaccc gcccgcgtag
    19141 ccggtcagtt tgccgtcgct gccgatgacg cggtggcagg gaatcaggat agatactttg
    19201 ttctgcccgt tggcggcggc aacggcgcgg acggctttgg ggttgcccaa acgctgcgcc
    19261 tgctccttgt agctgcgcgt ttcgccgtaa ggaatcgcca agagcgcgtc ccatgcctgc
    19321 ttttgaaact cggtgccaat ctgctccaaa ggcgtggcaa aggttttcag acgacccttg
    19381 aagtataagt ccaattcctg ccgcaaaagt tgcgtccgct catcctcccg aaacacaaac
    19441 cgtccgcgca aggctttttg gacggcggca atttcctgtt ccaaatgctt ctgtccgaca
    19501 aactccagca gacacaatcc cttatttccg aacaccgcca gcatctcgcc caaaggcgtg
    19561 gcaatggcgg cacacaccag ctcattcaaa ctgtcgggat aacgcgcttc caacagacgg
    19621 atggctcggc ggatacggac atattcttca ggcgcgcagc cgatattgtc ccaaaaatcc
    19681 cgatcgaact gcccggcttc gcattccgtc aaattgggat gcggcataac gccgcactca
    19741 caaacccggg tttcgagcca atagcggatt tcatcccatt ttgacggcag attgtttaag
    19801 gaaggcaggg taatcattcg tttgctccgt atctctatca tggatttgac ggcaaaattc
    19861 ccaattttcg ccattcccgc acgctgatgc aggaacgggc tacgacgtaa atcttgaggg
    19921 ttaggttgcg gcaatgccta aatatgcgat atttccaaag cctcagggaa aggaattaaa
    19981 gacttcagac gacctgcaaa ggctcgtctg aaaccacgat ttttgcattt gcgcattctg
    20041 gcacatcatc caaccgtttc ggcacattcc tgccgccgtt gacagcctat aatgaatcca
    20101 cttattcatc aagcaaagga atcatctatg caaacccgca ttctctccgc cgtactgctg
    20161 gctttttcaa ccgctgcctt tgccgggggc gcattcacgc tgcaattcga caacccgtcc
    20221 gaagacggcg gcttcacgca aaaccagctt ttgagcgcgc cttacggatt tggctgttcg
    20281 ggcggcaatg cttcgcccgc gctgtcgtgg aaaaatccgc ccgccgggac aaaaagtttc
    20341 gtcctgaccg tttacgataa agacgcgccg accggactgg gctggatgca ccgggtggtc
    20401 gccgacattc ccgccgatgt ccaccgccgc aacgcgacct cgctgcaatt aagccgctgc
    20461 gccaacatcg ccgacgacca gcccgcagcc atatcggcag taatcagttt gcggatttgc
    20521 cgcatcaggt tgacgccttc gtacacggca aaaccgatgc cgtcatgctg caaccacgcc
    20581 aacacgccgc aaagcgcggc ctccgccaca ttgtgcggca ctttttcatc cgccagcacc
    20641 gcagccgcat aatcaaacgc cgcgttcatc tgcctgggat gcggcagctt caccgcatcg
    20701 cacactgcct gcgccgtccc gtattgtgcg gcaaaccttt ctatggcttc ctgttcgaaa
    20761 gcaatccatt gcgcctgata gaggtcgtct gaatcgggaa tattgatgac gtcaaacgtc
    20821 tgcccgcctg ccaaggcgac cgcctcaccc gccgcaatcc tcaattcgcg cctcgctcac
    20881 cgcagcttct tgcttccgcg ccgcacgata agcacagccg gttcatatac cgccacgctg
    20941 cggtacaagg cggtatgatg ttgcacgatg ccgcccaaag cacccaatcg ttcgcgcgta
    21001 tgaaagtaat ttgacggcac tattatctcc aacacgcagg gccgcacata aggcgcagcc
    21061 ctgtttaccc tgatttggaa ggggttactt cccaaaaata aaccaaaatc ccactgcccc
    21121 gaatggccgg gtttcaacca tattccgtcg gtgtgcctgc aacttgactc cctcccctgt
    21181 gggggagggt tggggagagg gtaaaacggg gcagatacag acaatatttc cgttaccgcc
    21241 ccgatgccct ctccctatcc ctctcccacg aaagagggaa tggattgccg tttaaataaa
    21301 aaaatctaca taaaaatcaa tgttttatag tggattaaat ttaaatcagg acaaggcgac
    21361 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttgaatt
    21421 taatccacta tatctcaaac ccacattagg tctaatcaaa tggtcggaca tccatatccg
    21481 acagcccatt ttcttttcag acggcatttc cagccaacaa gcgcgccaat atcccctcat
    21541 acaccgcaga cagcttcgga atgtcgttta gccgcacgtt ttcgttgatt tggtggatgg
    21601 tcgcattgga cgggcctaat tcgatgagtt cttttgcaat ggctttgatg aagcgtccgt
    21661 ccgaagtgcc gccggtggtg gacaattcgg tctctacgcc gcagatttcg gcaatggcgg
    21721 cgcgtgccac gtcggtcagt ttgcccgcgt gggtcaggaa gggctgcccc gaacacgacc
    21781 actgcaaatc gtattgcaca ccgtgtttgt ccaaaatggc gtggacgcgt tgtttcagcc
    21841 ctgcttcggt ggactcggtg gagaagcgga aattgaattt gacgctcagc tcgcccggaa
    21901 tgacgttggt cgcgcctgtg ccgccgttga tattggaaat ttgaaagctg gttggcggga
    21961 aatattcgtt gccttcatcc cagacttcct gcgtcagctc taacaaggcc ggggcaaaag
    22021 tatggacggg attgattgcc aaatgcggat aggcaatatg accttgcttg cctttgacgg
    22081 tcaggttgcc cgacagcgag ccacgccggc cgtttttaat catatcgccc aatttgtcca
    22141 cggcggtcgg ctcgccgacg atgcagtagt cgataagctc gtcgcgcgct ttcaatacat
    22201 cgacgacttt ggtcgtgccg tccaacgcgt cgccctcttc gtcggaagta atcagaagcg
    22261 caatgctgcc ttggtggtcg ggatgttcgg caacgaagcg ttcacaggcg gtaacgaagc
    22321 aggcgatgct ggttttcatg tctgccgcgc cgcgcccgta taatcttccg tcgcgctcgg
    22381 ccggttcgaa cgggggcgaa tcccattttt cgacaggacc tgtcggtaca acgtcggtat
    22441 gccctgcaaa acagacgacg ggagctttcg tgccgcgtcg caaccagatg tttttggtgt
    22501 cgccgaaatg gagttcttca gccgcaaaac cgattttgtg caggcgttcg gcaaggagtt
    22561 tttggcaatc cctgtcgtca ggggtaacgg atggtcggga aatcagctct ttggcaagct
    22621 ctagggattg agtttcggtc atatttgttc acttttgaaa ttagaccgtc tgaaacgttc
    22681 tgaatgtgat tttcagacgg catttaggtt aggttggcat acggggtggg tattttaccc
    22741 atcagtcttc tgaatcattt gccgtggcag gcttcgtaaa gcggcagcaa atcttccacc
    22801 gtttccgcta tccatttcgc gacatcctgc ctgcccaaat cgtcgcgttc gatgtgtttg
    22861 ccgatgcaga aaaagtcttc gtcgttttgc aactttcggt cggactcgtt ttgttgcgcg
    22921 acggtgcggt agtcgtcgta ttcgctttcc gcaccgtgcc acatatcgaa agaagcgtat
    22981 ttttcggtat caaaattatc caaccagcgg ttgtaatcag gcagcgcaat cggcgacacg
    23041 tcggctttgt agcaatgcca gtccaagctg acactcaaac ggcggcggtt gagcagtatc
    23101 gacaaaatcg cggcggaatt tttatattgt tcgtatttga agtaggcaaa gaaatgggcg
    23161 cgaacctgcc agccgttgca ccagcgttcg atgtgcggcg gcgcaaacgg cgcacccaat
    23221 tcggcggcaa cctgctgaat cagctgctgc catatctgcc agttttcttt atagtcagcc
    23281 ttgatttgcg gaatgctttc aggctggtat tttttaagct gggaaaattg gaaaaacggg
    23341 atattgaaca aatcgcaact tttcggggtc agcataatat atccttgaga cgattgtttc
    23401 agacggcatt atttgcgccg gcgcgccgcc ataatttcgc cgatttcggt cagtttttct
    23461 tttgggataa aggtgttgcc catatcaaac agcggctctt caatcgccaa atgaacatca
    23521 tatcccgcca caaaacgttt gaacgcttcc tcatcgggga cataagcgtt gtctgcttcg
    23581 agtttggcaa attcggcgga aacagccgcc cagttgtcgt gcagcccgat atgttggcgc
    23641 aaaagctcgt ccacgctttc ttgggcttgc ggcgcatatt gcagcagcag cgggaagaag
    23701 ttttcttctt cgtcttcatg gtgcagcggc gcggcaacgt tgaaatactg ggcgatttgg
    23761 cggatggttt gcaaaacaat ctgattgcag ccgttttcgg cgatgtagtc cgacagcatc
    23821 gcgacttggc cgcaaaaacg gcgtactttg ccgtggcagg catacagcat ttcaatcggt
    23881 tcggcaaagg taacgctttg ggtttcaaac ggattcatgt tttcgttctc aacgggcact
    23941 tttcaagcag tcattttata ataaaacagc ctgcacaaag caggctgtcc gtcttttgag
    24001 actttaagcg gattaatcga ccaaagtcac tttgccgttc atcaaagcac cgtgacccgg
    24061 gaaggtgcaa gcgaatttgt attcgccgtc agccagtttg gcaggatcca gagtcaaaga
    24121 agattcttcg ccgccaccga tcagtttggt gtgggcaaca acgcgcgcat cgtcaggttt
    24181 aacgtagtca gtgtcagctg cgccaacgcc atctttgaaa ataccgtcca tgtcttcagt
    24241 tttaccgatg acaatgttgt gacccatgct ggttttaggt tgggtaccgg tgtgtttcag
    24301 ggtgatggtg aattccttac aagctttgct tacttggatg tctttagtgt tgaactgcat
    24361 attgtcgttg gattcgacag ttgccgcaca gttgccggca gcaggggctt cggcagcatc
    24421 tgcaggagca gcttcggcgg caggcgcttc ggaagcgggt gcttcagcag caggagttgc
    24481 ctcggcagca ggcgcggcag gttcttgaga gcaggcagcc aaaccgataa cggcggcaga
    24541 aatcagagcc agatacgctt tcataacaaa tctccaatcg ataaaataat attcggtttt
    24601 acagaaatca aagtgcaacc gccattaaca aaaccttgaa aaagattccg ccgcgttgca
    24661 caaacagatg tttcggagcg gcattttgct acaaatttca tttgaaatca aagcctgtct
    24721 gcaagtttac aatcgtttac tttaaaaggg gcaattttac cccgaaccta tttctttagt
    24781 attagaccca ttatccttta cttcttaata ttaacggatg tttacacaaa ttcccgtata
    24841 cattttatgc gccatgcctt ctaaccaagt ttgccaatgc ctccgccaat tcgggatgcc
    24901 gtttttccaa ctttaccgcc gccgaaccga aactctccag cgcagcctta ctcaaatgca
    24961 gggtattggt tttcggcggt ttttccggtt tcgggaccag cctgaccgaa acagagcgta
    25021 tcgaagcgtc aagccctgcc aactgcggca ataccgacgg tgcaatcatt ttcaagcgcg
    25081 atgccgccat attgtttgcc gccaaaagga caagcctgcc gtcttcgata catgccgtct
    25141 gaaaatgcgg gtgcaggttg gcaggcagca gttttttcac ggcggcatcc aaccgccgcc
    25201 actgtcccgc ctgtttcaaa agtccggaaa gcagcgcgtc ccgcctgccc aactgttcca
    25261 aattcataaa acatacaccc aaaaagattg aaataccgca aacgcgcctt tatttcagac
    25321 ggcattagca ctttgcacaa acgcttgtgt taaaatcgcg ttttcgccca ctattatatc
    25381 aggcgcagga attattcatg ctgacaaaca ttgccaagaa aatcttcggc agccgcaacg
    25441 accgcttgct gaaacaatac cgtaaatccg ttgccagaat caacgcgctc gaagaacaga
    25501 tgcaagccct aagcgatgct gatctgcaag ccaaaactgc cgaattcaaa caacgcctcg
    25561 ccgacggtca gactttggac ggcattttgc ccgaagcctt cgccgtctgc cgcgaagcgt
    25621 cccgccgcac cctcggtatg cgccacttcg acgtgcagct tatcggcggt atggtgctgc
    25681 acgacggcaa aatcgccgaa atgcgtaccg gcgaaggcaa aaccctggtc gccaccctcg
    25741 ccgtctatct caacgcgctg gccggcaaag gcgtacacgt cgttaccgtc aacgactacc
    25801 tcgcctcacg cgatgcgggc attatggagc cgctctacaa tttcctcggc cttaccgtgg
    25861 gcgtgattat ttcagatatg cagccgttcg accgtcaaaa cgcctatgcc gccgatatca
    25921 cctacggcac caataatgaa ttcggcttcg actacctgcg cgacaatatg gttaccaacc
    25981 aatacgacaa agtgcagcgc gaattgaatt ttgccgttgt cgatgaagtg gattccatct
    26041 tgattgacga agcgcgcact ccgctgatta tctccggcca ggcagacgac aacatccagt
    26101 tgtaccaaat catgaacacc gttccgcccc acctcgtccg tcaagagaca gaagaaggcg
    26161 aaggcgacta ttgggtcgac gaaaaggcac atcaggtcat cctgagcgaa gcaggtcacg
    26221 aacacgccga gcaaatcctg acccaaatgg gattgctggc agaaaacgac tccctctatt
    26281 ccgccgccaa tatcgccctg atgcaccacc ttatggcggc attgcgcgcg cattccctct
    26341 tccacaaaga ccaacattac gtcatccaag acggcgaaat cgtcatcgtg gacgaattta
    26401 caggtcggct gatgtccggc cgccgctggt ccgaaggcct gcatcaagcc gtagaagcca
    26461 aagaaggcgt ggaaatcaaa cgcgaaaacc aaacccttgc ctccatcacc ttccaaaact
    26521 atttccgcct gtacaccaag ctctccggta tgaccggtac ggcagatacc gaggctttcg
    26581 agttccaaag catctacaac ctcgaaaccg tcattatccc gaccaaccgc cccatacagc
    26641 gcaaagactt caacgaccag attttccgtt ccgccgaaga aaaattcgaa gccgtcgtca
    26701 aagacatcga ggaatgccac aaacgcgggc agcccgtcct cgtcggtaca accagcatcg
    26761 aaaactccga actggtctcc caccttctgc aaaaagccgg actgccgcac aacgtcctca
    26821 acgccaaaga acacgaacgc gaagcactga ttgtcgccca agccggcaaa gtcggcgcga
    26881 ttaccgttgc caccaatatg gcgggacgcg gtacggacat cgttttaggc ggcaacctga
    26941 agcaccaaac cgatgccatc cgtgccgacg aagccttgag cgacgaagag aaacaggcac
    27001 aaatctccgc actcgaagac ggctggcagg cagaacacga caaagtgatg gaagcaggcg
    27061 gtttgcacat catcggtacg gaacgccacg aaagccgccg tatcgacaac cagttgcgcg
    27121 gacgttccgg ccgtcagggc gaccccggat ccagccgttt ctacctctcg tttgaagacc
    27181 cgctgctgcg cctattcgca ctcgaccgcg ccgccgccat cctcaaccgc ctcgcccccg
    27241 aacgcggcgt tgccatcgag cacaacctgc tgacgcgcca aatcgaaggg gcgcaacgca
    27301 aagtcgaagg cagaaacttc gatatgcgca aacaggttct ggaatacgac gacgtcgcca
    27361 acgagcaacg taaagtcatt tacagccaac gcaacgaaat tttaaccagc aaagacatca
    27421 gcgacctgat gaaggaaatc cgttctgatg tcgtcagcga cctcgtggat acctatatgc
    27481 cgcccgacag catggaagaa caatgggaca tcccgacttt ggagaaccgt ctggctgccg
    27541 aattcagact gcacgaagac atccaatcct ggctgaaggc ggacaatgcg attgacggtc
    27601 aagacatcaa agaacgcctg atcgaacgca tcgaaaacga atatgccgcc aaaaccgaac
    27661 tggtcggcaa gcaggcaatg gccgatttcg agcgcaacgt gatgttgcag gtcatcgaca
    27721 accaatggcg cgaacacctc gccgctatgg actacctgcg acaaggtata cacctgcgca
    27781 gctatgccca aaaaaatccg aagcaggaat ataaacgtga agcctttacc atgttccaag
    27841 acctgtggaa cggcatcaaa ttccatattg cctccctgct tacctcggtt caaatcgaac
    27901 aaaaccctgt cgcggtggtt gaagagcaac ccatcggcaa catccagtcc atccattccg
    27961 aatcgcccga tatggaagaa cttttgggtc agtcgcaaac cgatttggtt accgaagcct
    28021 ttaatcccga tgggacagat ttcagccccg aagccttgga agcgcggggg caaatcgtcc
    28081 accgcaacga cccctgcccc tgcggcagcg gtttgaaata caaacaatgc cacggcaaac
    28141 tggcttaagc gtttgaacgc aaatgccgtc tgaacatccc gctcccgttt cagacggcat
    28201 tttgcctgaa ccgccacatc cgactgccat tccgaaaaat cccgatttcg taccgtccgt
    28261 accaaaaaca gacatcccgt ccgccccaca tcatgattcc atccgacttc attgacgagc
    28321 ttttagccaa aaccgatatt gtcgatatta tcgacgagca ggttccgctg aaaaaaggcg
    28381 gggcgaacta tatggcgtgt tgcccgttcc acaaggaaaa aacgccgtcg ttttcggtca
    28441 gtccaaccaa gcagttttac cattgtttca gttgcggggc gcatggttcg gcgattggtt
    28501 ttgtgatgga gcatcagggg ctgtcgtttc cggaggctgt gcagtttctt gccgaccgtg
    28561 tgggcatggt tgtgcctaaa gtgcgtgggc agaacgataa tcccgaagtc cgtgccgaac
    28621 gtaagaaaaa aaagcagacg ctggaagaaa cgacggcggc ggcggctgat ttttacgcgc
    28681 aacagctaaa attcaatccg gcggcaaaag cttatttgga caagcgcggc ttgagtgcag
    28741 aagttatcgc gcattatggt ttgggctacg cgcccgacgg ctggcagcct ttggcgcaag
    28801 tgttccaacc gtatccgaat accgcgttgg tggatacggg gatggtgatt gacaatgagg
    28861 ggcggtatta cgaccgcttc cgccatcgga ttatgttccc tatccgcaat ccgcgcgggc
    28921 aggtcatcgg tttcggcggc agggtgctgg acgactccaa acccaaatat ctaaactctc
    28981 ccgatacacc tttgtttgac aaagggaaaa acctttacgg cttgtatgag gggcgtgccg
    29041 ctgtcaagga agcggagcgg attctggtgg tcgaaggcta tatggacgtg gtcgcgctgg
    29101 cacagttcgg cgtgggctac ggtgtggcgg ccttgggcac ggcaacaaca gctgaacacg
    29161 tcaaaatcct aatgcgtcag gcagacagta tttatttctg tttcgacggc gacagcgcgg
    29221 ggcgaaaagc ggcttggcgc gcgctggaaa acgcgttgcc gcagttgaaa gacgacaaat
    29281 cgctgcattt tttgttcctg cccgaggaac acgaccccga cagttacatc cgtgcctacg
    29341 gcaaagcgca atttgaagac gcgcttctga atcaaagcaa gcctttgtcg gagtatttct
    29401 gggaacacct ttcagacggc attcatctca atacgcagga aggcaaggcg gaattggtga
    29461 aaaccagttc gccgcttttg gcgcagatta ccgcgccggc attggcttat ttgttaaaac
    29521 aacggcttag cgaactggtc ggcatcgacc ccgacaacct cgcgcaactg ctaggacagg
    29581 aagcgccgaa gcggcacgtc aaacaaaaaa actacaaact gcctccgatt tccgtcaaac
    29641 agcccgtcat gctgacgctg gtacagcggc aaatccgcag cctcttgata aatccggatt
    29701 gggctgcata tatagacctg cccgattatc tggcgttgga cggtgatttc gcctgccttg
    29761 ccaatcttgc cgaatcgatt aaaaaccatg ccgccgtacc cgaaaccgct caggttttag
    29821 agtatatgcg cggctcgcct tacgaagaaa cgataacccg aatcttccat tcaacgcacc
    29881 aatcggaaga aatgaacagc agcagtgaag aagattgcga gaatttccaa atcggcatga
    29941 aaaaactgct caatgagtta aaatacagcc aaatcgaagc attgaaacaa aaaagcctgc
    30001 aatccggctt aaatgaaagc gagaaaaaac ttttgctgtc gttgctgacc gcaaaacaaa
    30061 attgaccggc ggattccgcc acccgtaaac ccgttatgcc gtctgaaaag cacttacccc
    30121 ggctgcaaca acgacacctg cagaacaccc atcccaaaaa gccttcagac ggcatcagag
    30181 tacccgctct gccacgcctt caggtgcgtt caaacgcaaa ccgtcggcac cttaccaaca
    30241 gaaagcagac aatgtccaga aaccaaaatc acgaagaata tcaagacgac acccgtccgt
    30301 taagcattga agagcaacgc gcgcgcctgc gtcagctcat catcatgggt aaagaacgcg
    30361 gctacatcac ctactccgaa atcaacgacg ccctgccaga cgatatgtct gatgccgacc
    30421 aaatagacaa tatcgtcagc atgatttccg gtttgggcat ccaagttacc gaacacgccc
    30481 ccgatgcgga agacatattg ttaagcgaca atgccgccgt taccgacgat gatgccgtcg
    30541 aagaagccga ggccgccctt tccagtgcag attccgagtt cggcagaacc accgaccccg
    30601 tccgtatgta tatgcgcgaa atggggcagg tcgacctgct gacccgcgaa gacgaaatca
    30661 tcatcgcaaa aaaaattgaa aacgccctga aaaatatggt tcaggccatc tccgcctgcc
    30721 cgggatccat tgcggaaatc ttagaactca tcgaaaaaat ccgcaaagac gaaatccgcg
    30781 tcgacgaagt cgtagaagcc attatcgacc cgaatgaagt attgctcaac gaattgggct
    30841 tggggcactt ggaaaccaca gcgcccgaga aaccttccaa cgacaattcg gacgaaaacg
    30901 aagacgacga agaatcggaa gaagatgcgg atgaaatctc ggcagccaat ctcgccgaat
    30961 tgaaacaaaa agtcatcggc cactttgccc aaatcgaaaa agactacaaa aaaatgattg
    31021 gccgtttgga aaaacaccac agccggcaca aagactatct cgcctaccgc gacgcgattg
    31081 ccaacaaact gctggaagtc cgtttcgcca cccggcaaat cgacagcctc agcagcagcc
    31141 tgcgcgggaa agtagaaaac atccgcaaac tcgaacgcga aatccgcgac atctgcctcg
    31201 accgcgtcca tatggaacgc gactacttca tccaaaactt cctgcccgaa atcaccaatc
    31261 tagaatggat tgaagaagaa atcgccaaag gcagggtttg gagcgacgcg ctcgaccgct
    31321 tccgccacgc catcctcgaa aaacaaaccg agttggcgga tatggaaaaa gaaacccgca
    31381 tttccatcga agagttgaaa gaaatcaaca aaaatatggt gtcaagcgaa aaagaaaccg
    31441 cagccgccaa acaggaaatg attcaggcaa acttgcgcct cgtgatttcc atcgccaaaa
    31501 aatataccaa ccggggctta caattccttg atctgattca ggaaggcaac atcggtttga
    31561 tgaaagcggt cgataagttc gaataccgca gaggctataa attctccacc tacgcaacct
    31621 ggtggatccg ccaggcaatt acacgctcga ttgccgatca ggcgcgtacc atccgcattc
    31681 cggtacatat gattgaaacc atcaacaaga tgaaccgcat ctcgcgccaa caccttcaag
    31741 aaaccggcga agaacccgat tccgccaaac ttgccgaact gatgcagatg cccgaagaca
    31801 aaatccgcaa aatcatgaaa atcgccaaag agccgatttc gatggaaacc cccatcggcg
    31861 acgacgacga ttcgcacttg ggcgacttca tcgaagatgc caacaatgtt gcgccggccg
    31921 atgcggcaat gtacaccagc ctgcacgaag taaccaaaga aatcctcgaa agcctgacac
    31981 cgcgtgaggc aaaagtcctg cgtatgcgtt tcggcatcga tatgaacacc gaccacacgc
    32041 tggaagaagt cggcagacag tttgacgtaa cgcgcgaacg catccgacaa atcgaggcaa
    32101 aagcactccg caagctgcgg catccgacaa gaagcgaccg tttgagaagt ttcttggaca
    32161 gcgaagacag caagctgtaa accaaaaaac cgcaggtttc aaatacctgc ggttttttct
    32221 tacacaataa acaacgcttc cacatatccc acactcctat cccgagacct ttgcaaaatc
    32281 ccccaaaatc ccctaaattc ccaccaagac atttagggga ttttccatga gcaccttctt
    32341 tcagcaaacc gcacaagcca tgattgccaa acacatcgac cgcttcccac tattgaagct
    32401 agatcaagtg attgattggc aaccgatcga acaatacctg aaccgtcaaa gaatccgtta
    32461 cgtccgagac caccgcggcc gtcccgccta cccgctgttg tccatgttca aagccgtcct
    32521 gctcggacaa tggcacagcc tctccgatcc cgaactcgaa cacagcctca tcacccgcat
    32581 cgatttcaac ctgttttgcc gttttgacga actgagcatc cccgattaca gcaccttatg
    32641 ccgctaccgc aaccggctgg cgcaagacaa taccctgtcc gaattgctgg aactgattaa
    32701 ccgccaactg accgaaaaaa acctaaaaat agagaaagca tccgccgccg tcattgacgc
    32761 caccattatt cagaccgccg gcagcaaaca gcgtcaggcc atagaagtcg atgaagaagg
    32821 acaagtcagc ggccaaacca caccgagtaa agacagcgat gcccgttgga caaagaaaaa
    32881 cggcctctac agactcggtt acaaacaaca tacccgtacc gatgcggaag gctatatcga
    32941 gaaactgcac atcaccccag ccaataccca tgagtgcaac cacctgttgc ctttgctgga
    33001 aggcattacc gaaggcacga ccatctatgc cgacaaaggc tatgacagtg cggaaaaccg
    33061 aacatctgga agaacatcag ttgttagacg gca
//
LOCUS       DAWFKN010000013        32965 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFKN010000013 DAWFKN010000000
VERSION     DAWFKN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.013"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(338..4507)
                     /locus_tag="WGR46_000621"
     CDS             complement(338..4507)
                     /locus_tag="WGR46_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0855617.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WGR46_000622"
     CDS             complement(4562..6409)
                     /locus_tag="WGR46_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0855618.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WGR46_000623"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WGR46_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0855619.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WGR46_000624"
     CDS             8315..9808
                     /locus_tag="WGR46_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0855620.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGE
                     VLHFHTENGRSYPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGN
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9884..10099)
                     /locus_tag="WGR46_000625"
     CDS             complement(9884..10099)
                     /locus_tag="WGR46_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855621.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(10092..10307)
                     /locus_tag="WGR46_000626"
     CDS             complement(10092..10307)
                     /locus_tag="WGR46_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0855622.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGR46_000627"
     CDS             complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGR46_000627"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0855623.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            12052..12192
                     /locus_tag="WGR46_000628"
     CDS             12052..12192
                     /locus_tag="WGR46_000628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855624.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            12189..12989
                     /locus_tag="WGR46_000629"
     CDS             12189..12989
                     /locus_tag="WGR46_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0855625.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            13029..14015
                     /locus_tag="WGR46_000630"
     CDS             13029..14015
                     /locus_tag="WGR46_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0855626.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14672..14934
                     /locus_tag="WGR46_000631"
                     /pseudo
     CDS             14672..14934
                     /locus_tag="WGR46_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14972..15346)
                     /locus_tag="WGR46_000632"
                     /pseudo
     CDS             complement(14972..15346)
                     /locus_tag="WGR46_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15519..15875)
                     /locus_tag="WGR46_000633"
     CDS             complement(15519..15875)
                     /locus_tag="WGR46_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0855627.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(16018..16362)
                     /locus_tag="WGR46_000634"
     CDS             complement(16018..16362)
                     /locus_tag="WGR46_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855628.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16521..16921)
                     /locus_tag="WGR46_000635"
                     /pseudo
     CDS             complement(16521..16921)
                     /locus_tag="WGR46_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16925..17665)
                     /locus_tag="WGR46_000636"
                     /pseudo
     CDS             complement(16925..17665)
                     /locus_tag="WGR46_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17735..18052)
                     /locus_tag="WGR46_000637"
     CDS             complement(17735..18052)
                     /locus_tag="WGR46_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855629.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(18049..18660)
                     /locus_tag="WGR46_000638"
                     /pseudo
     CDS             complement(18049..18660)
                     /locus_tag="WGR46_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18744..19046)
                     /locus_tag="WGR46_000639"
     CDS             complement(18744..19046)
                     /locus_tag="WGR46_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855630.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(19225..19431)
                     /locus_tag="WGR46_000640"
     CDS             complement(19225..19431)
                     /locus_tag="WGR46_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855631.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGR46_000641"
                     /pseudo
     CDS             complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGR46_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20572..20916)
                     /locus_tag="WGR46_000642"
     CDS             complement(20572..20916)
                     /locus_tag="WGR46_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855632.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20954..21298)
                     /locus_tag="WGR46_000643"
     CDS             complement(20954..21298)
                     /locus_tag="WGR46_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855633.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(21301..21489)
                     /locus_tag="WGR46_000644"
     CDS             complement(21301..21489)
                     /locus_tag="WGR46_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0855634.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21559..21894)
                     /locus_tag="WGR46_000645"
                     /pseudo
     CDS             complement(21559..21894)
                     /locus_tag="WGR46_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(22000..22371)
                     /locus_tag="WGR46_000646"
     CDS             complement(22000..22371)
                     /locus_tag="WGR46_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0855635.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(22368..22619)
                     /locus_tag="WGR46_000647"
     CDS             complement(22368..22619)
                     /locus_tag="WGR46_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855636.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(22751..22957)
                     /locus_tag="WGR46_000648"
     CDS             complement(22751..22957)
                     /locus_tag="WGR46_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855637.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGR46_000649"
                     /pseudo
     CDS             complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGR46_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(23909..24121)
                     /locus_tag="WGR46_000650"
     CDS             complement(23909..24121)
                     /locus_tag="WGR46_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855638.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(24197..24556)
                     /locus_tag="WGR46_000651"
     CDS             complement(24197..24556)
                     /locus_tag="WGR46_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0855639.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(24561..25277)
                     /locus_tag="WGR46_000652"
                     /pseudo
     CDS             complement(24561..25277)
                     /locus_tag="WGR46_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(25494..25877)
                     /locus_tag="WGR46_000653"
     CDS             complement(25494..25877)
                     /locus_tag="WGR46_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855640.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGR46_000654"
     CDS             complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGR46_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0855641.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGR46_000655"
     CDS             complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGR46_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0855642.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(28474..29193)
                     /locus_tag="WGR46_000656"
     CDS             complement(28474..29193)
                     /locus_tag="WGR46_000656"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0855643.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            29289..29396
                     /locus_tag="WGR46_000657"
                     /pseudo
     CDS             29289..29396
                     /locus_tag="WGR46_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(29518..30372)
                     /locus_tag="WGR46_000658"
     CDS             complement(29518..30372)
                     /locus_tag="WGR46_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0855644.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGR46_000659"
     CDS             complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGR46_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0855645.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(31430..31639)
                     /locus_tag="WGR46_000660"
     CDS             complement(31430..31639)
                     /locus_tag="WGR46_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855646.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            31717..32298
                     /locus_tag="WGR46_000661"
     CDS             31717..32298
                     /locus_tag="WGR46_000661"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0855647.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            32359..32949
                     /locus_tag="WGR46_000662"
     CDS             32359..32949
                     /locus_tag="WGR46_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0855648.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         7951 a         7545 c         8048 g         9421 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccta aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttaatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc accgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ttcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcgacggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggcacgaa ggcggacgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcagccttca
     3901 ggtcgaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttgtcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgaac
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg tggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaactaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atattttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggca aaccggcaaa aggcgaaatg cttgctggca cggcagtgta
     9481 caacggcgaa gtgctgcatt tccatacgga aaacggccgt tcgtacccga ccagaggcag
     9541 gtttgccgca aaagtcgatt ttggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg
     9661 gaattggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggtc cggccggcga
     9721 ggaagtggcg gggaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ttgatgccgt ctgaagcgag ggcgttcaga
     9841 cggcattgtg atgtttgggt cgcgggtccg gttcaagtcc gcatcagttg ataaacccgt
     9901 cgtctttcat tttcttttcg gaaaaatatt gaccgcatac cagtaaggcg gcgaggatga
     9961 tgacaaacag ccccgccata gtcagtgtga ttttgaagaa caattccgct tcgagaaaag
    10021 aaaaccaaag ttgcacaatc agcagcagca cccagccggc aaacagtccg agacaggcat
    10081 aaacgccgag cttaaacacg gtcccgctcc aaaatgacga cgtaggcctc gcgtttgaaa
    10141 aacaacagca tacggcgcaa atctttttcc atcgttacaa cccgccagcc ttcgggggtt
    10201 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    10261 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatagg attcctttta
    10321 acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgcaa aacagcaaac
    10381 cttatttctt attgcccaac tgcggcaata ccgaaccttc gcccaatgcc aacaaaccgc
    10441 tttgcgagta aatgcccagt ttggcgcggg tgtcggtaat gtcgaggttg cgcatagtca
    10501 gttgtccgat acggtcgagc ggtgtgaacg ctgcgtcttc gactttttcc atactcaggc
    10561 gttcgggttg gtaggtcagg ttgggcgatt cggtgttcag aatcgagtag tcgttgccgc
    10621 gacgcaattc gagggttact tcgccggtaa tggctttggc aacccagcgt tgggcggttt
    10681 cgcgcaacat cagggcttgg ctgtcgaacc agcggccttg gtagagcaaa cgtccgaggc
    10741 gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggataccg gtcaccaaac
    10801 gctcgtaggc gatgtggaac aacgccattc ccggggcttc gtagatgccg cgcgatttgg
    10861 cttcgatgat gcggttttcg atttggtcgc tcatacccag accgtggcgg ccgccgatgc
    10921 ggttggcttc gaggaagagt tcgacgggat cagcgtattc tttgccgttc agtgcaaccg
    10981 gcacgccttc ttcaaagcgc acgctgactt cttcgggttt gatttcgacg ttttcgtccc
    11041 agaaggcaac gcccataata ggtttgacga ttttgatgcc cgagttcaaa aattccaaat
    11101 ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggaataggct ttttccaccg
    11161 acattttgta gttgaaaccg ttggcaatca aaaattcgct catttcatga cggccgccga
    11221 gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg ggattggtga
    11281 gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg ccgtcgcccc
    11341 aaatattcac atcgtcttct ttcatggcgg aaacaagcat agtgccggtt acggcgcgac
    11401 ccagaggcgt ggtgttgaag taggcgatgc cgccggtgga aacgtggaac gcgccgcatt
    11461 ggatggcggc gatgccttcg tgtgccagct gtgtgcggca gtcaatcagg cgggcgtttt
    11521 ccgcaccgta ttccattgct tttttgggaa tggcattgta gtcgtcttcg tcgggctggc
    11581 cgaggttggc agtgtaggca taaggtagcg cgcctttgag tttcatccac aacagcgcgg
    11641 cagaggtatc aagaccgccg gagaaggcga tgccgacttt ctgaccgacg gggaggtttt
    11701 gtaaaatagt atggttttgg ctcataaggg tatccttcgg acttggaaaa aggagttcgt
    11761 cgtgtttcct tttcgattga aaccccgcca cttggacatc tgtccttcgg ggcggtagaa
    11821 tcagacttta tttgggaggg gcgtaatccc ttccaaatca ggacggcaca tagggcggtg
    11881 ctttatgtgt cgtcccgtgt gttgaaacat tatcttataa atataaccca ttgtcagccg
    11941 ttaaggctgt ctgaaaagaa gctttgcccg tttcgctgaa aatcttttca gacggtcttt
    12001 acgccatcct caacctgact ccctcccctg cgggggaggg ttggggagag ggtgcagaca
    12061 gcagccctta agcaaacgct gcctgaaaaa cgcaagctcg accctctccc taaccctctc
    12121 ccatgggaga gggaacaaat cacagcacca tcgacatttt cagacggcat cggtaaagaa
    12181 acccatctat gaacgcgttc aacctcacca tcatcggcga cgaaatctta cacggcagcc
    12241 gccaagacaa gcatttcgcc tttttcaaat ccctgctcga atccaaaggg ctgaagctca
    12301 atcaggtgca atatctgccc gacgaacccg atttgctggt caggcaactg cgccgcagct
    12361 tttcagacgg cataccgacg tttgttaccg gcggcatcgg cgccacgccc gacgaccaca
    12421 cgcgccaagc cgcagcggct gctttggatt tgcctgtcgt ccgccatcct gaagccgcca
    12481 agtttatcga aggcgtaacc cggaaacgcg gtgagccgct cgattcgccc gaacacgccc
    12541 aacgcctgaa gatggcggat tttccccaag gcgcggaatt ggtgcctaat ccgtttaata
    12601 atatcgccgg attttccatt cgcgagcatt atttcttccc tggttttcct gtgatggcgc
    12661 acccgatggc ggaatgggtt ttggatactt attacgtcgg ccgcttcaac caaaccaaac
    12721 gcggcagccg cagcgtgtat gtgtttgagc agcccgaatc gcgcattacg ccgcttatgg
    12781 agcatatcga gcaaacttat cccggtgtgc gttcgtacag cctgcccagc gtcggttgga
    12841 cgcattcaga cggcacgcag gtcaaaccgc acattgagtt cggcatcaaa gccgaaggag
    12901 aagccgtcaa ccttttggac gcggcgtggg cggaagtgtt gcacagcttg gacggattgg
    12961 gagcagagct gaaaaatcgg gtaaactgaa aaccgtttgc ccaaatcgga cgatatttaa
    13021 ggaaaaccat gcaataccga atcagaagag aaaacgaagc acccgaagca aaaaatacgg
    13081 gggaaacctt atgggagcgc gacatcatgc gcgaagtgct gctgtccgcc tatcaggaca
    13141 ggcgcaggga gcggatgtgg aaaaacatct ggcgtgccgt cagcaccctg attctggttg
    13201 ccctgattgc aggcattttc cgaaaagacg aagccgcatt gcagttggcg ggcaacacgc
    13261 cgcataccgc agtcgtcaac ctgtacggcg aaatcggcaa cggtgtagaa gaccaggtca
    13321 aaaaactcaa agacggtatg gaggccgcct acaaaaaccc gcaggcaaaa gccatcgtca
    13381 tccgcgccaa cagccccggc ggttcgcccg tcgtctccaa caccgctttt gaagaaatac
    13441 gccgtctgaa ggcgcagcac cccggcattc ccgtttatct cgtggcggaa gatatgtgcg
    13501 cgtccggctg ctactacatc gcggcggcgg cggacaaaat ctatgccgac ccgtccagca
    13561 tcgtcggcag catcggcgtt atcggcagca gtttcgacgc gaccggcctg atggaaaaaa
    13621 tcggcgtgaa acgcagggtt aaaattgcag gcagcaacaa aggcatgggc gatccgtttt
    13681 cgcccgaaac gcccgaacag tcgaaaatct gggaagaaat gctgaccggc atacacggcg
    13741 aattcatcaa agccgtcaaa accggacggg gcggccgcct caaattccga caatatcccg
    13801 acgtgttcag cggtcgcgtg tacacaggcg cggatgcgct gaaagtcggg ctggtggacg
    13861 gactcggcaa tatttacagc gtggcgcgag acgtggtcaa agcccccgat gtggtggatt
    13921 acactccgaa ggacgatttc ggcagaatct tgggcagacg cttcggcgca gaactcaaag
    13981 caagcgtgcg cgaagccctg caggccgtcc ggtagcgttg tttacagtat ttctaaggga
    14041 ataccaaaat aaaaatatac acaaaaacac tcacctcccc agtatgctgc cactaagtca
    14101 gccgcgtcac tatttaccgt gcacttaaag ccgtaaaagg caggttgtcc ccgggaggtt
    14161 ttgtgcatcc tgataccgat cggttgttgt tgctcaaagg acagaaggcc gctgataaac
    14221 gagattacct gtttgtcgct attgacgatt tttatactct gccattttgc cagacaaaac
    14281 cgcagacagt gctgccaagt ttctgaccga acatctggcc gacccctgct tgtacctgat
    14341 tgagtacgtt tactctgaca atgataggta atataaagag ccgtccaaca tgctttcggt
    14401 gcagcttgtt ataataatgg gattggttgg aggcttgccc gatttgcttg tccgcagacc
    14461 aacggtaagg cggagcgggt tatccgtacc ttgatggaga tgtggcatga ggaacagtcg
    14521 tttgacagtc cggaacatcg gcaaaaggag ttgtgctgct ttattaattt aggggctacc
    14581 ctagatcact agggaaattc aaattaagtt agaattatcc ctatgagaga aagtcgtcta
    14641 agccggtata aacaaaataa actcattttg ttatcgtggc tatgatgtat tggatgcggg
    14701 cgaatttagc catttccgta tcaatcacag cacacatttt gccgaacgac aaaaccatat
    14761 taatggaact gagaactttt ggaatcaagc aaaacgtcat ttacgcaagt ttaacggcat
    14821 tcccaaagcg cattttgagc tgtatttaaa ggagtgcgaa cgacgtttta acaacagtga
    14881 gataaaagtt cttgttccat tttaaaacaa ttagtaaaat caagtttgtc ctagttgtcc
    14941 agggcagcct catctcttta tagatagctg cttaccaagt atccttaatt gctaattcaa
    15001 gccaatcttc aaaactatta aaaaattcat ggttttctat attatctaaa ttaatgacaa
    15061 taaccctaga atttcctgtt acatcattac catcaaaata agcttcccat tctccattac
    15121 tagcgaatgg gataagattt aaatcaggca ggatagaatt tctaaatata taagctactt
    15181 ctgccccttc tgttccataa ttctcaaacc accagtgaaa attatgtggc tgaatataag
    15241 gtattaaaga tgtatcgtta gagatacgaa catacttttc aggataacaa aacctttcag
    15301 gaagcatatt actaggatat aaattaaatt cattttaaat aatcatttcg ttaccttagg
    15361 atcataaata ctaccattag gaaactgggt agaccaattt ggtggtgcta gttcattttc
    15421 aatttgtttt ttaattctaa attgaatagc ttctccccat gtttgcggca tctaatgctg
    15481 cttgctctgc atcaatatta ctaaaaattg gattagtctc aaaaatgaaa aattactgtt
    15541 ttttcatttt ttatagcata ttcaagtaca tgaataatat ttttcactaa gggctctgta
    15601 gaaaaatctg aattttgtaa tttttctttt acagccaaaa tactttcctt atctattagc
    15661 caatccccct cacttggtcc aaataaccaa ttatcggtaa tttcagaaac aagtctccat
    15721 aatccacgat tttgatattc aataatttga ctttccgtaa tttctacaac tgtttcagat
    15781 tgaatgtttt tataagccca atcaatcatt tcagtatatt cactaattga aacattgtct
    15841 tttaaacaga aaattaacag gttgtgttca ttcatttgtc taagttctta atatgagttt
    15901 gttgttgtaa ataatctttt gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg
    15961 gatgatgccg cgaatatcat ccatacggtt accccaccaa attttattgg tgtatcgtta
    16021 atcatttatt tcaaaaaagt tatttattat catattatca gggagcaggt cgttaaaaaa
    16081 aaccccctca aattccatta aattagtatt tatccagcac ataaacgaac caaagcgttc
    16141 tttccctgag tgtaaatatt taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt
    16201 taatggtttt tctatagtga aaaaaaagcc cttactcgtt ttagttcgag atactatttt
    16261 aaataaattt ttattttcaa taaaataaga atattcattt actaagttgt tatgataaaa
    16321 aagtaatttt attaaaatgt tttcataatt ttctaggttc atttctagct caatttaaaa
    16381 tgatttcatt gttgccgtac caatgcgaaa tttaccatca acaaaatttc ccttcactga
    16441 tacaggtacg ccatcaactt gggtagtttt attttcaggc tgcctgaaag aaagataggc
    16501 aacttattac tcatcttacg ctagccctca ataattgaat acgttgcact agaaccaatt
    16561 atttctatga atgtttgctc aaacataaaa atatagtatt taaagaattc tctattcaaa
    16621 ggaaaataac tttctttcaa tatccattcc gcataacact catcaagaag ttcaaaaaag
    16681 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
    16741 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
    16801 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
    16861 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
    16921 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
    16981 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ctatcaggta
    17041 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
    17101 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
    17161 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcacct
    17221 taccggtttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
    17281 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
    17341 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
    17401 agccggtatt tttaacccaa tcccatttag tcgggttaag ttctactttt ttacctctcc
    17461 aaaccgtacc tgcggcctct gctacggaaa gggcagtttg ggctgttgca gttatattcg
    17521 gatgggcatc agcccattgt ctggcagagt tgatactgtc ttttaccgca aaagcactgt
    17581 cttgtaatgc ggttgcggcc gcaagttgtg cttcgggact taaatttcct aaattattaa
    17641 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
    17701 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
    17761 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
    17821 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
    17881 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
    17941 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
    18001 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
    18061 atatccagaa tgatgacgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
    18121 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
    18181 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagcctcatc
    18241 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
    18301 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
    18361 cataggtcgg gcagcaggtt ttttatagcc ggtattttta acccaatccc atttggtcgg
    18421 gttaaggttt acttttttac cgccccaaac cgtacctgcg gcctctgcta tggcaagagc
    18481 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
    18541 gctgtctttt accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc
    18601 tggacttaat tttcctaaat cattaatacc ttgtagagtc tgctgagcgg ctgtatctga
    18661 gccggtcagt gtaggttgga ttaaaaccca gcattttcaa catcttgatg ttttactata
    18721 aatctattct tgtgcttcct ttgttagata aatttcaaca caaaagatac gtatgcattc
    18781 tctaatttta tttgagataa ataatgcatt gctttgtcga tactgttaaa taaattactt
    18841 ccctcgtaat accaattatc atatgtttga taaaaataat tatctttctt gatataaata
    18901 tctcccccca atacaagtat tctctcattg ataaataaat ttaatatatc tattgcaata
    18961 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
    19021 taaatttcaa aaatttcatt taacatgtta atctctatct actatttttc aatcggtata
    19081 aaccctattc acattttatt tttcctaaaa ccaaatcttc gactaactgg gttgcataaa
    19141 taatatcatt tatgatttta acaggtaaat tatttgatag gtaaccatgg gattccgtca
    19201 tcattgattt ctctaggtgc ttatttatct gattggtaag ccttttgcgt aagcttcttt
    19261 cgcctccgca agtgtcattt cattaggtcc accatctata ttagtttctc ctgtattttg
    19321 ccactgacaa acgtcacaga tatcatagtc cataacttca gttccgcaaa caggacaatg
    19381 tagccatatt tctccgtcaa tttcccatgt cttttttgaa ttctccatca taatacttct
    19441 ttcctaagtt tggtttaaac attgttttga ttttgaatgt ttttggattg gccaaaacat
    19501 agatattgtt attgatatca tagcgcactc gtctatgttc cgtctgataa cctaatatct
    19561 tgtccgatgt ctgttttgat aacaaatcag acgccatttt ttggtagtct tcaatggtta
    19621 tatcgccaaa ttcttttcca tgagctttga aatgtccatt taaagatttc tcagtaggaa
    19681 acttggactt tgtccatctg atgcggtctt gaattgttcc tgtcggagaa atagatgtat
    19741 caggtttaac atatgaggca ggtaagccga tattgttatc agagtgtgta gggtgttgct
    19801 gcgtacttgg ttttggtggc ttattcccac ctgccatctc cccatctaaa gtctgcatag
    19861 ggcgggcagt aggtgtttta tagccggtat ttttaaccca atcccatttg gtcgggttaa
    19921 gttctacttt tttaccgccc caaaccgtac ctgcggcctc tgctatggca agggcggttt
    19981 gggcagtagc tgtaatgttc ggatgggcat cagcccattg tctggcggaa ttgataccgt
    20041 cttttaccgc aaaagtactg tcctataatg cgcttgcggc agcaagttgt gctttcggac
    20101 ttaaatttcc taaatcatta ataccttgta aagtttgctg cgcagagatg taggtcggat
    20161 atttgtatcc gacaaaaata tttgacgcat ctatcgtttc cgaaatgccg ctgttggaaa
    20221 atgtcggatt cgagaatccg acctacggct atttcgttta agtcataaat agccattcat
    20281 gttccttctg aatttgcttt tgctatcctt agaaccaatc atataaacat tatcatcatt
    20341 aattaatgtt ctaagtttat gaagcggtca aatctgtcaa actaccagag atgaagcata
    20401 tcctcctgga agtaattctg ttcgtgctgt ttccatatct tctttgccat catcagttac
    20461 ttctctgtct aaataaaatc ttacgataag tttttgtcta atttcatcaa attctgccgt
    20521 aattaatctt atattgatat gaatacacgg ttcatacctg ctattagcca gtcaggtgta
    20581 gttggatggg gattcatttt taataatatc ttcaagcaag tgaaatgtaa gaaaagcgta
    20641 gatacttgaa ggccggctat ctgattggca aaaagaaatt tcggagataa agaatttccg
    20701 tttatatttt tcataaatta aatcaacacc ttgttttaca taaaaactaa cttcctcagg
    20761 aaggatattt ataacattct ctccttttac ttttatagtt aatggtttga tttttatgtt
    20821 ttcatgaata tctgatagga ttatttctaa aaaattattt ttatttccag tcattctggc
    20881 tattttatgt atatttccat tgtgttcgat tatcatttta ttttttccaa ggatttcctt
    20941 ctatattgat agtttatcta aagatttcat ttaaaatatc ctgactaatt tcttgtttat
    21001 ataattctac aattttctgt ttctgtgtat ttgtataaaa tttttcgttg gtatctgctt
    21061 gatattttaa ttgtctcaat atttccatga tttcatattc tgatttttca tcatattctt
    21121 caatcatgta atcaacaaat tgttttagaa tatcaaaggc ttcgtaactt tccaaaattt
    21181 taatatgttt atcggcaaaa ttggacatat accattcgtc agtgattgga ttagtattta
    21241 aatcatctaa aaaaacattt aaatcatcta aaaaaacatt tacatcatct aaaaacatga
    21301 ctaatcctta aacttaaaat ctgttttctc accggttctt gagttttcaa tgtaatgtat
    21361 ttcaattttg tttacgtttt gaacacgctt aacccaaccg tcttttgcta accaaccatt
    21421 tttagtatcg gacataggag gtattctcgc aggagttttg ccctgtgggt ttgccttaac
    21481 ttcttccatt gctaattgtt cctttaaatt attaggtatt gtacgaccgg ttgatttttc
    21541 aacagattgt gtccctattt ttaatgattt gtttccccca gccatttccc catctaaagt
    21601 ctgcataggg cgggcagcag gttttttata accggtattt ttaacccaat cccatttggt
    21661 cgggttaagg tttacttttt taccgcccca aaccgtacct gcggcctctg ctatggcaag
    21721 agcagtttgg gctgttgcag ttatattcgg atgggcatca gcccattgtc tggcggaatt
    21781 aatgccgtct tttaccgcaa aagcactgtc ctgtaatagg ctcgcggcag caagttgtgc
    21841 ttccggactt aaatttccta attcattaat atcttgtaaa gtttgctgcg cagagatgta
    21901 ggtcggatat ttgtatccga caaaaatatt tgacgcatct atcgtttccg aaatgccgct
    21961 gttggaaaat gtcggattcg agaatccgac ctacggctgc taccaaccat cattttcagc
    22021 ttcttttagc caagtatcaa aattttcaaa agtctcgtaa tattttgtat tatctaaatt
    22081 aactactata attttaggat tacctgattt atctgaaata tcaaaacagg cagcccactc
    22141 ttggtttcta gcaaaaggta ataggtttgg aatgccagtt atttcaaaat aaatgtcaat
    22201 tacttctgat atattgcttt ctgcattttc aaaccaccaa ggaaaagaat attgttcatc
    22261 gtagttaatg gcatgagtgg attgagctaa ttttaaataa ctttgcggat atttaaatcc
    22321 tttaggaagc attgcttcag gatacggaac aaaactattt tgttttatca tttcctaagt
    22381 acctttacat cataaatact tccgttagga aatcttttgc tccacccttc tggaactcct
    22441 ttttgtgttt cttgctttct aattcgtagc tggatagctt ctccccaagt ttgaggctca
    22501 ataccatcat gcttaaacct gattctctct tttgatgata atcggtcatt aaaattatga
    22561 acattcgctt ccacttttac tcctgcttca cgtgctgcca taatacgcgt gttatccata
    22621 ctagtaggtg ctccatcagg catattaaca acatcaaccc tgtcgccaac ccatgatttt
    22681 tttctttcat ttcggctaca atagaatcat agttgtaatt tgcactaggc tctgacaaaa
    22741 atttgggttg ttaagttgtc atttttttat aaattccagc aaactgttgt tgtaaattat
    22801 ctacttcatg ttttctaaca ccagatacat tttcaaattc tcttgaatct acacatacgc
    22861 ccgcatatat ctcattcaag atagctcgta aaacaaaaac ttcatcttga gttagtgaaa
    22921 tagttgagtt ttgattattt atggatagta tattcatggc tttttcctat aaatcatcat
    22981 aatatttttt acctgatgtt ggtctaaatg ctgtacctcc atcgtcagaa tttttatctc
    23041 ggataactat tgtccctgtt ttatcatccc aatacgcagt tctaccgcga ggtaactctt
    23101 tcatatttgt tggatggcta acaatatttt caatatgccg agcaaaatct gctggtgaat
    23161 tgatatttaa atccgtaaat tcttgttgtc ttatgacatg cttgttataa gcatgcccac
    23221 tactaatttg ttctccaatt agttgcgctt gtaaagacgg ttgtgtggaa gcatctgctt
    23281 tgctgttgga cgttatagat ttaggcggtc tattcccacc ggccatttcc ccatccaaag
    23341 tatgcatggt gcgaacagca ggtgttttat agccggtatt tttaacccaa tcccatttgg
    23401 tcgggttaag ttctactttt ttaccgcccc aaaccgtagt tgcggcctct gctacggcaa
    23461 gggcagtttg ggctgttgct gttatattcg gatgggcatc agcccattgt ctggcggaat
    23521 taatgccgtc ttttaccgca aaggcactgt cctgtaatag gctcgcggca gcaagttgtg
    23581 cttccggact taattttcct aaatcattaa taccttgtag agtctgctga gcggctgtat
    23641 ctgtgacagg gcttaccgca ctgtctgtaa ttgccccaat ccctactcct tgaaaaccca
    23701 ttaaaaaagg attaaccgca ccttggacga tgccgcgaat atcatccata cggttagccc
    23761 accaattttt attggggtcg ttttcccata tcagttttcc tgcttcatca gcacagttag
    23821 gattggcatt gatgcccacc cagtttcagg cagcctgaaa aggattttta atatacggca
    23881 aaaggtgggc aacaagtgcc caccttacct agctaacatt tatcccaagt atcatatatt
    23941 tttttaagtt ctttatccaa agaaagaact ttattttttc aacaccatga attatctcaa
    24001 aatctctaca atccacgcat acgcctgaat aaatttctcc tacaattgct tgaataatat
    24061 acaactcatc tttatcgaga ctaattttaa ctgaactctc ttcaaaatta attaatttca
    24121 tgaataattc cttttatttt aattcgtcat caaaataccg tttgcctgtt tttggtctaa
    24181 atgctgtcca ccatctttag tcttggatat ttagattata atcgtcaaat tcccagtcaa
    24241 aatattttgc caaatttgat atagacttat cttcagacgt tgaattacat atttccaata
    24301 tatcatttat caattttaat atgtgttcaa tattaaaacg gtttgtaaca attgagtttt
    24361 ttaatgacat cggtctatgt atatttgaaa gtaaccattc agtgctatat atataaacat
    24421 aaaaataatc agcaccatta ctttcatcaa aacctattga taatgtcaaa agtatacaaa
    24481 aattttctaa cttgtcttgt gggaaatctt ccaaatcaaa gtcagaacaa tcataatttt
    24541 ttaattttaa tctcatataa ttacctaata tttaaatgtc catttttaat tttattcctt
    24601 tttccatttg aatcaatagt ataagtttca aaatttgctt gaatacctgt agttgcaaat
    24661 tgtgaatttt tttctgttgg tggccgatat tgtcgagtgc catctatact taaccatccg
    24721 cctccactag tttgtcttgc accctcacca acccaaattt tacctagcct atctgcctct
    24781 tcataagttg cagttcctat tggaaaattt ttttcactct tatgaagagc tagactcaat
    24841 cttggatctt gagccgcaat gttttttaag ttttgcccag ttaattgatt aactaattga
    24901 ggataagttg cagcattggc ttttcctcca gacgttatag atttaggcgg tttattccca
    24961 ccggccattt ccccatctaa agtctgcata ggtcgggcag caggtgtttt ataatcggta
    25021 tttttaaccc aatcccattt agtcgggtta agttctactt ttttaccgcc ccaaaccgta
    25081 cctgcggcct ctgctatggc aagggcagtt tgggctgttg cagttatatt cggatgggca
    25141 tcagcccatt gtctggcaga gttgatactg tcttttaccg caaaagcact gtcctgtaat
    25201 aggctcacgg cggcaagttg tgcttccgga cttaaatttc ctaaatcatt aataccttgt
    25261 agagtctgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat atttgacgca
    25321 tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat tcgagaatct gacctacggc
    25381 tgctgactat tttcttgttt atataattct acaactgcaa actactatca aatcttttta
    25441 tctctggctc tacactcagc aagaatattt ttattttaca aaacataggc tagttatttg
    25501 agtatattgt caaattctaa tttgtttttt tgatagattt tttttatacc attaatacat
    25561 tcattagttg taattctacc aagcgaatgt aatttatcaa tatcaaaaca atcggtataa
    25621 accctattca cagttttatc ttgtatttcc ttatgaatac ttaatatatt gagatagttt
    25681 agaaatttta ttttctcaga ttgaggtaac tcatcaaaac caagccaata ataaaaattg
    25741 ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc ttcaactaac
    25801 tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga tgggtaacca
    25861 tggggttccg tcatcatttg taactcctgg tacatgtgcg tgtcctgtaa gtgcgcgtgg
    25921 gtcttgatgg gtatttgcat gatgagcatc atctattctg gctccagtca agtttccatt
    25981 actatctcgg acttggataa aacgagcatc aggcgaacct gttatataac cgcctattgg
    26041 tgttggtatt gcatttcctt tgctgtctac ataaattgtt cctgtcggag aaatagatgt
    26101 atcaggttta acatatgggg caggtaagcc tatattgtta tcagagtgtg tagagtgttg
    26161 ctgcgtactt ggtttcggtg gcttatttcc cccagccatc tccccgtcta cagtctgcat
    26221 aaggcgaaca gcaggttttt tatagccgtt atttttaacc caatcccatt tggtcgggtt
    26281 aagttctact tttttacctc tccaaaccgt acctgcagcc tctgctacgg aaagggcagt
    26341 ttgggcagta gctgtaatgt tcggatgggc atcagcccat tgtctggcag agttgatact
    26401 gtcttttacc gcaaaagcac tgtcttgtaa tgcggttgcg gccgcaagtt gtgcttccgg
    26461 acttaaattt cctaaattgt gaataccctg taaagttttc cgtgctgccg cataggttac
    26521 cgggtttacc gcactgtctg taattgcccc aactcccaat ccctgaaaac ccattaaaaa
    26581 aggattaacc gcaccttgga cgatgccgcg aatatcatcc atacggttag cccaccaatt
    26641 tttattgggg tcgctttccc atatcagttt tcctgcttca tccgcacggc tgaaaaaacc
    26701 agaggcggca ccggcatttt cttttagcca acggtctgaa aatggggctt gagggacaat
    26761 attagtcttt gtttttgttg aagttccttt gacataatag ctgtatatat cccttgctcc
    26821 tccggggggc ggataatcgc tgccttgcgg cccgtcatat ccatcctccg gatgaatttc
    26881 cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg tctacaccgc cgctgaaatc
    26941 agaagtgctt tttgaatcat gattatcgaa cggactgtgt acttcatgtc catgtcctga
    27001 aaaatgggtt tcataaccga taatgccttc aaaccctgtc cgttcgtgtg taataggcag
    27061 tacaggactg accgcagttg catcaaatgt ctggacggcg taaacccgat ttttaacact
    27121 gccgcgagca ttaccaaaca gatggtattt accacccggt tcgtagtgtt ttgcctgcat
    27181 atcatcgcgt aaacgggcat ccaaaccgtt cgcatgacta atggggattt gcagcaacga
    27241 tattgccgct gcaaacagca tcatgaattt ttgaataggc aatttcattt tgttttcctt
    27301 ggtagcaagc ggttatggtc gtgtgaatca aggttgccct tgtctatgtt gtcgcactac
    27361 ttcatcgctg tatccatacc cctcatgact gttatcagtc tctacggatg gggcggagtt
    27421 acccgtatga ttgccgtatg gtcggatatc ggagaaatcg accattaatc cttccgtcgg
    27481 tttaattcct ttgcttactt tatacggccc catccacaat gcgtaatttt ctttataggc
    27541 agcttcaaac gcattggttt tgggtttgat gagcaatttt ttattggttc tgtctactgc
    27601 gaaatattcc agttttgttt gggctttcag tgtttcggca ttgtataggt gcatttcggt
    27661 tctgttgcgt atcgttccga atacgtcgat gttaataaac acatctgtat cggcattggc
    27721 aggagaaaca acgtctatgc cgcgcaggaa aaatacggtc tgtaccaagt gggaaagaaa
    27781 ggcagtgtcg cgcgggttag tcgtcaaggt ttcatttcga taatccccca tcccgccaat
    27841 atttaagccc agactgctcc tacttccgct accgtctgat tgggtgcgcg agcgtgcagg
    27901 ggcattaagt gtagataaag aagtggttaa acctgtcaaa ccgcctgatg ttgtttcagc
    27961 ggtggtttcg taacgtggat aggtgtaatc ggtacggacg gcagggctgt ttatgtattc
    28021 gccacgaatc agtgcatcaa tggagtagcg accccctgtc aaactgcctg aaccttggtc
    28081 gcccatagtt gcaatgtaca atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc
    28141 tttaacggca gctctggcag aagcggccac aagttcttgt tcgaccgcaa agcgtttacc
    28201 tccgccatgc gatggaatac ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa
    28261 aagaataggt atcagcagcc gtgcttgcat agattttctc ctttgatgaa aaacaaattg
    28321 tatcaaaatt gtaaatatag tggattaaat ttaaaccagt acggcaaggc gaggcaacgc
    28381 cgtactggtt taaatttaat ccactataat ctccatataa taaatggctg tctgaagccc
    28441 atatgcgact atatctgccg acactatggt caatcagcag tgaaccagcg taccttcgtt
    28501 ttcaccggtt actacacgtt tgagcgagcc ttctttagca atgccgaata ctacaatatt
    28561 gagcttgcgt tcgcggcaga gggcgaaagc ggtggcatcc atcactttga ggtttttcaa
    28621 caaggcttcg tcaaaagtaa tggtttcgta gcgcgtggcg gatgagtctt ttttggggtc
    28681 tgcggtgtac acgccgtcaa cgttggtggc tttgagcatc acgtcgcagt tcatttccgc
    28741 accgcgcaat gcggcggcag tgtcggtcgt gaagaacggg ttaccggtac cggcggcaaa
    28801 aatcacgact ttgccttctt ccaaatattg gatggcttta ggacgggcgt aagtttcagc
    28861 gatttgctgc atagacagtg cggattgtac gcgcgctttg atgcctaaag tttcaaatgc
    28921 gtctttgagt gccaacgcgt tcattacggt cgccatcatg cccatgtagt cggcggtggc
    28981 gcgatccatg ctgcctgctt gggcggatac gccccggaaa atattgccgc cgccgacaac
    29041 aataccgact tgcacgccca ttttaacgac ttcggcaatt tcgccgacag tttgaacgat
    29101 ggtatcgtga ttgatgccga acggatcgga acccatcagg gattcgccgg agagtttcag
    29161 taatacgcgt ttgtatttga tttgctgtgt catgggatac cttgctttct tgaatgtcgt
    29221 cggtcggagt ttgggccgtc tgaattttca aacggtcgga attacaatga tatggtgtgt
    29281 cgttctgtta tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
    29341 aaatagtacg gcaaggcgag gcaacgctgt actggtttaa atttaatcca ctatattgtt
    29401 ttgttttcag acggccttta tattcggtcg tcttaaaata ggtaccgcaa acggttttct
    29461 caaaaaaaag caccctgatt cgtttggaat ccaggtgctt tctttttcat aagtgcctta
    29521 cactttggcg gctgcggcta cttcggctgc gtagtcgaca acggctttct cgataccgtc
    29581 gcctactttg tagcggacga agctgaccac ttcagtgccg ttttctttgg cgaattgggc
    29641 aacagtttga tcggggttca tcacgaatgc ttggccgttc agagtgattt cagccaagaa
    29701 tttgcggata cggccttcaa ccattttagc ggcgatgtcg gcaggtttgc cggaagcgat
    29761 ggcttgctcg gtgtagatgt ggcgttcttt ttcaacggtt tcggcatcta cttcggcttc
    29821 gcttacgcat tgtggtttag cggcaacaat atgcataccg attttgcgtg ctacgtcttc
    29881 agagcctttg tactcaacca atacgccttc ggtcgccaat gcgccgtgga tgtaggcaac
    29941 cagttggttg gcagtgtcga tcacttggaa gcgacggaca gacatattct cgcccaattt
    30001 ggcgatgatg gctttgcgtt ctgcttcaac cagttcgctc agttcttcaa cagaagccgg
    30061 ttttttctcg gcagcagttt tcgcaacgaa gttggcaaat tctacgaagc ccgcgtcttt
    30121 agcaacgaag tcggtttcgc agtttacttc gaccaatgcg ccgacattgc cgttgatcgc
    30181 gtaagccaat acgccttcgg cagcggtacg gccggccagt ttaccggctt tcgcaccgga
    30241 tttgatacgc aggatttctt cggctttgtc gaagttgcct tcggcttcaa ccaaggcttt
    30301 tttgcattcc atcatgccca ggccggtagc ggcgcgcagg tcggcaacca tttttgcagt
    30361 aatttctgcc attttgaatc tcctagattt ttgggaaata cggaaaggat tttccgcatt
    30421 cgggaatcgt gtgccgcgaa cggacgttca gacggcatat ttgagaaaag gggcataacg
    30481 cccctcttcg gtttgccgga ttactcggca gcggcttctt gggcagcggc tacggtttct
    30541 tgcagcgctt ggtttttgcc ttccaaaact gcgtcagcga tgccgcggca gtacaggcgg
    30601 atggctttgg cggagtcgtc gttgccgggg atgacgtatt tcacgccgtc agggctgttg
    30661 ttggtatcga ctacggcgat aacggggatg cccagttttt cagcctcgac cagagtacct
    30721 ttttggtagc cggtatcgat aacgaaaatc gcgtcgggca ggcctttcat gtttttgata
    30781 ccgcccaaag aacgttccag tttttcaaca tcgcgttgca tttccagaat ttcttttttg
    30841 ctgaaaccgc tttcggcagc attttccaag gctgcggttt tttcttccag gcgtttgatg
    30901 gattgcttaa cggttttgta gttggtcagc ataccgccca accaacggtg atcgacgaaa
    30961 ggcatgccgg cgcgggtcgc ttcttcgcgg atgatgtcgc gggcttggcg tttggtacct
    31021 acgaacaata ctgtaccttt gttggcaacc agacgacgta cggcttcttg cgcgtcttgg
    31081 aacatcggca gggttttttc caggttgacg atatggattt tgttgcgcgc accgaaaatg
    31141 tattgagcca ttttcgggtt ccagaaacgg gtttggtggc cgaagtggac accggcttca
    31201 atcatctgac gcatagtaat ttgagacatt ttgatttcct tgaaagggtt aagggcacac
    31261 attcaatcgc atagaacttg atagaccgcg cctattgtca agcacccttc ggcgaaagtg
    31321 ggcataagtt ttaaaaaata aaagagtgtt tccgaaatgg aaaactgacg gattatagcg
    31381 gataagaggt cgtctgaaaa gggattcagg gttttgtgcc gctgtctttt taccgttgtg
    31441 cacgctgctg ttcgaattct gcctgttcct gttgcttcat caggcggttg cggcggcgga
    31501 tggtcacgac aaagaccgtg aacacggtgg gcagcaccgc ccaaaaaacc aaataaatca
    31561 aggcgcgcgc aatactcggc tgcgcggcgg aaaacatcac ggcaacaaaa agatagccga
    31621 tggcgacaat gtgccacatt gtgtgcgtcc tgtttgaatg ttaacaaaat cgttataatc
    31681 gcaacattct accgcaatcc tgaaaggccg tctgaaatga ggaacgagga aaaacacgcc
    31741 ctgcgccgcg aattacgcgg gcggcgttcg caaatggggt gggacgtgcg ggcggcggca
    31801 acggtaaaaa ttaaccgcct gctcaaacgt tatatcaagc acggtcggaa aatcggcgtg
    31861 tattggccga tgggcaagga attgcgtttg gacggctttg tccgcgcggc gcaaaaacgc
    31921 ggcgcaaaac tttatctgcc ttatatcgaa ccgcgttcgc ggcggatgtg gtttacgccg
    31981 tatcctgaaa gcggaatgga acgggagcgc atacggggca gggcgaggtt gaacgtgccg
    32041 cagtttgcag ggcgcaaaat ccgcgtgcac ggtttgtcgg tattgctcgt tccgcttgtc
    32101 ggcatagacc gcgaaggcta ccgtttgggg caggcaggcg gctattacga tgcgacgctt
    32161 gcagcgatga aataccgttt gcaggcaaaa accgtgggcg tgggctttgc ctgccagttg
    32221 gtggacagac tgtcggtcga ggcgcacgac ctgccgctgg acggttttgt gtcggaggcg
    32281 gggatattgt gcttttagcc cttcagacgg catttcgggc ggaaatgccg tctgaaggcg
    32341 gaatattggg agaaaaggat gtcggaaagg ataatactgc cggtcttgtc attgggcggc
    32401 gttcggctcg aaccgttgga tgtgcatcat gaaacgggtt tgcgcgaggc ggtttgcgat
    32461 ggggaggttt ggaagctggg ggtaacgtcc gcgccccatc cggatagggt ggcagactat
    32521 atcgggacag cattggcaac gcgtttggcg tttgcggttg tcgatgaaga ggcgggcagg
    32581 gtggtcggga caacggcgta ttatcatttt gaaccgcaga ttccgcgttt ggatatcgga
    32641 ttcacatggt atgcggcatc ggcacggcgg acacggataa acacctgttg caaaatcatg
    32701 ctgttggatt acgcatttga tgttttggct tgccgttgcg tgggatggcg cacggatatt
    32761 ctgaaccttg cctcgcaacg tgccatcgaa cggctgggtg cggaaaaaga cggcgtgctg
    32821 cgtatgcata tgctccgtaa agacggcagt gtgcgcgata cggtcgtgta cagtatgctg
    32881 agggaagatt ggtgcaaaaa cagggaaatc ctgaccggca ggttggcagg gtatggcgtg
    32941 caggtgtgag gcagtatttt caaat
//
LOCUS       DAWFKN010000014        31930 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFKN010000014 DAWFKN010000000
VERSION     DAWFKN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.014"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(329..604)
                     /locus_tag="WGR46_000663"
     CDS             complement(329..604)
                     /locus_tag="WGR46_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0855649.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(601..1884)
                     /locus_tag="WGR46_000664"
     CDS             complement(601..1884)
                     /locus_tag="WGR46_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0855650.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGR46_000665"
     CDS             complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGR46_000665"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0855651.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3542..3691
                     /locus_tag="WGR46_000666"
     CDS             3542..3691
                     /locus_tag="WGR46_000666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855652.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            3769..4578
                     /gene="aroE"
                     /locus_tag="WGR46_000667"
     CDS             3769..4578
                     /gene="aroE"
                     /locus_tag="WGR46_000667"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0855653.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4580..5281
                     /gene="mtgA"
                     /locus_tag="WGR46_000668"
     CDS             4580..5281
                     /gene="mtgA"
                     /locus_tag="WGR46_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0855654.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5606..5681)
                     /locus_tag="WGR46_000669"
     tRNA            complement(5606..5681)
                     /locus_tag="WGR46_000669"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5646..5648),aa:Phe,seq:gaa)
     gene            complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGR46_000670"
     CDS             complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGR46_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855655.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGR46_000671"
     CDS             complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGR46_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0855656.1"
                     /translation="MIQKICKLFVLIAFFSVSPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGR46_000672"
     CDS             complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGR46_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0855657.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7769..8305)
                     /locus_tag="WGR46_000673"
     CDS             complement(7769..8305)
                     /locus_tag="WGR46_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0855658.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8523..9497)
                     /locus_tag="WGR46_000674"
     CDS             complement(8523..9497)
                     /locus_tag="WGR46_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0855659.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9594..10649
                     /gene="tal"
                     /locus_tag="WGR46_000675"
     CDS             9594..10649
                     /gene="tal"
                     /locus_tag="WGR46_000675"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0855660.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            10791..11081
                     /locus_tag="WGR46_000676"
     CDS             10791..11081
                     /locus_tag="WGR46_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855661.1"
                     /translation="MAKALEIISPNEIYSDLIFKNPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            11098..11985
                     /gene="gluQRS"
                     /locus_tag="WGR46_000677"
     CDS             11098..11985
                     /gene="gluQRS"
                     /locus_tag="WGR46_000677"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0855662.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPCQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(12055..13065)
                     /gene="dusA"
                     /locus_tag="WGR46_000678"
     CDS             complement(12055..13065)
                     /gene="dusA"
                     /locus_tag="WGR46_000678"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0855663.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(13096..13587)
                     /locus_tag="WGR46_000679"
     CDS             complement(13096..13587)
                     /locus_tag="WGR46_000679"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0855664.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13661..14419)
                     /locus_tag="WGR46_000680"
     CDS             complement(13661..14419)
                     /locus_tag="WGR46_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0855665.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAHQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14490..15356)
                     /locus_tag="WGR46_000681"
     CDS             complement(14490..15356)
                     /locus_tag="WGR46_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0855666.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15405..15683)
                     /locus_tag="WGR46_000682"
     CDS             complement(15405..15683)
                     /locus_tag="WGR46_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0855667.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15683..15973)
                     /locus_tag="WGR46_000683"
     CDS             complement(15683..15973)
                     /locus_tag="WGR46_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0855668.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(15976..16506)
                     /locus_tag="WGR46_000684"
     CDS             complement(15976..16506)
                     /locus_tag="WGR46_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0855669.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16814..19339
                     /locus_tag="WGR46_000685"
     CDS             16814..19339
                     /locus_tag="WGR46_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0855670.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19512..19988)
                     /gene="gloA"
                     /locus_tag="WGR46_000686"
     CDS             complement(19512..19988)
                     /gene="gloA"
                     /locus_tag="WGR46_000686"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0855671.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(19952..21121)
                     /gene="lapB"
                     /locus_tag="WGR46_000687"
     CDS             complement(19952..21121)
                     /gene="lapB"
                     /locus_tag="WGR46_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0855672.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21132..21446)
                     /locus_tag="WGR46_000688"
     CDS             complement(21132..21446)
                     /locus_tag="WGR46_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0855673.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            21756..22754
                     /gene="ilvE"
                     /locus_tag="WGR46_000689"
     CDS             21756..22754
                     /gene="ilvE"
                     /locus_tag="WGR46_000689"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0855674.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(22998..23783)
                     /gene="fabI"
                     /locus_tag="WGR46_000690"
     CDS             complement(22998..23783)
                     /gene="fabI"
                     /locus_tag="WGR46_000690"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0855675.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(23938..24759)
                     /gene="dapD"
                     /locus_tag="WGR46_000691"
     CDS             complement(23938..24759)
                     /gene="dapD"
                     /locus_tag="WGR46_000691"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0855676.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(24769..24999)
                     /locus_tag="WGR46_000692"
     CDS             complement(24769..24999)
                     /locus_tag="WGR46_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855677.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(24969..26612)
                     /gene="pgi"
                     /locus_tag="WGR46_000693"
     CDS             complement(24969..26612)
                     /gene="pgi"
                     /locus_tag="WGR46_000693"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0855678.1"
                     /translation="MDAFTRAWCALERHYQDTRHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLERLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27122..28354
                     /gene="pilG"
                     /locus_tag="WGR46_000694"
     CDS             27122..28354
                     /gene="pilG"
                     /locus_tag="WGR46_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0855679.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28428..29288
                     /locus_tag="WGR46_000695"
     CDS             28428..29288
                     /locus_tag="WGR46_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0855680.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29290..29922
                     /locus_tag="WGR46_000696"
     CDS             29290..29922
                     /locus_tag="WGR46_000696"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0855681.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            29915..30124
                     /locus_tag="WGR46_000697"
     CDS             29915..30124
                     /locus_tag="WGR46_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0855682.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(30209..31885)
                     /gene="pilB"
                     /locus_tag="WGR46_000698"
     CDS             complement(30209..31885)
                     /gene="pilB"
                     /locus_tag="WGR46_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0855683.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7448 a         9004 c         8213 g         7265 t
ORIGIN      
        1 gttgacatat tgtgcgatat cgagggctgt accgtatttt cccatcgtat tttcccgttc
       61 tttatatgag cggcaggcat cctcataaaa gaagggatac cgataaggaa aaataccccg
      121 gcataaaaaa tatactgatt aatcaataca aatccaacaa acagttcatc gtattctttt
      181 ctgttggcgt ataaaaaata ctgatatatg ccttcacttt catagggcga cgctttatgt
      241 accgtcctgt gtgttgaaac gtcggttttc cttaccgcct gccccgcttt tccctgattg
      301 ctgccaactg ccgtccgaac ctgaaccgtc aggtttcaga cggcattttt tctgcctgct
      361 cccacaattg ccgtttttgt gcggcggtca gttttttgac ggcgatttcc tgcctgagtg
      421 ccgttccttt atccatcccg cctgcaacga tacgcatcgc caccggtttg aatacgcggg
      481 tatatttcgc gcctttaccg gttgtgtggg cggcaagccg ctgttgcgga ttcgggctga
      541 tgccgcaata gaacgcgctg ttttcacaca atatcaggta aacgctccag tttgacgcgt
      601 tcatctgcct gcatcctgag ttttctcccc gttccaaggc gcaactttca gcagcagcag
      661 catacccggt agggcgagtg cgaaacacag ctggaaaaac ggtacatacc cgagccattc
      721 gatcagataa ccggcaaagg aattgatgac cgtacgggga acagcggaca ggctggtaaa
      781 cagcgcaagc tgcgttgcgg taaatgcggg attggtttca cgcgccatat acgatacgaa
      841 cgccgccgtc cccaaaccca cgccgactgc ctctgcgccg ataactgccg ccagcatcag
      901 cctctcgcct gtgccgaccg tgtcgaaatg tccgaaccct gccagccata caaaccccaa
      961 aacggttaca gcctgcaccg cgccaaatat ccacaaggct ttgtttacgc cgattttcag
     1021 catccacacg ccgcccaaga tacctgccgc cactgccggc cacagtcctg catttttcgc
     1081 aatcaaaccg atgtctgtct tgctgaaacc catatccaga taaaacggcg ttgccaacgc
     1141 ggttgccata ctgtcgccga gtttgtaaag gaagataaac agcagcacgc acaccgccga
     1201 agcgatgccc ttgcgcgtaa aaaattcttt aaacggctct accacggtct gcttcaacgt
     1261 tttaggaacg gcaggcggca acacgggttc gcgcgcaaga aacagcgtca tcagaaggcc
     1321 gggcagcata aacagcgaag tgatgacaaa cacctccggc cacggcatcc tgtctgccaa
     1381 caccaaactc aatgaaccgg gaatcagggc ggcaatccgg taggcgttca catgaaccga
     1441 gttgcccaaa cccaattctt cgtctgacaa aatctcgcgc ctgaacgcat ccaatacaat
     1501 atcctgactg gcggaaaaaa aagcgacaag caccgacaag ccggcaatca gcggcagatg
     1561 attacggggg tttaaaaagg cataggccgc caaagccgcc agcaaccctg cctgcgtcag
     1621 cagcatccac ccgcgccgcc gtcccaaaac gggcagcctg accgcgtcca tcagcggcga
     1681 ccacaaaaat ttccaagtaa acggcagacc gattaacgcc atcagcccga tgctcttcaa
     1741 atccacctgc tcgctgcgca accacgccgg aatcaggtta atcagaaagt acagcggcag
     1801 ccccgaggta aaccccgtaa aaatacagac aagcatattt cgggaaaaga tttgcccgat
     1861 aaaacctgat ttcgatgcag tcatcgttcg tgcagcccaa aaaaaacaaa agcctattgt
     1921 agcaaatatc gcccgcctgc tgaaaaatgc cgccccgcgc cgccggtttg ccgttcaaat
     1981 gcaaaatgcc gtctgaaact gtgttcagac ggcattgctt ttctgaaaac cagacgtgct
     2041 tacaggctgt aatacatttc aaattccagc gggtgcggcg ccatgcggat gcggcgtacg
     2101 tcttcttctt tgaaagcgat gtagctgtcg atccagtctt tgctgaacac gccgccgcgc
     2161 aggaggaatt cgtggtcggc tttgagggcg gccagtgctt cttccaaaga agcgcaaacg
     2221 gtcggcacca atgcatcttc ttccggcggc agatcgtaca ggtttttatc ggcagggtcg
     2281 cccggatgga ttttgttttg aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc
     2341 aaatacgggt tggcggtcgg atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg
     2401 ttcacggacg gaatgcggat ggaagcggaa cggtttttgg cggagtatgc cagtttggtc
     2461 ggcgcttcaa agtgcggcac gaggcgtttg taggagttgg tggacggatt ggtaatcgcg
     2521 ttcaaggctt tggcgtgttt gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag
     2581 ccggcatagc cgtcgcctgc gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg
     2641 tgcataccgc tgccgttgtc gcccataatc ggtttgggca taaaggtggc tgttttgccg
     2701 aagttgtggg caacgttttg aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg
     2761 accaaggtgg cgaaacgcgt gccgatttcc atttggctgc cggtaccgac ttcactgtgg
     2821 tggacttcga cttcgatgcc gagttcttcc aaaatgttta ccatcgcgga gcgcaggtct
     2881 tgtcccgcgt caatcggagc aacgggcgcg taaccgcctt tgacggcagg gcggtggccg
     2941 gtgttttgac cgtccatatg cagaccgctt gcccacgcgc cgctttcgga cgtgatttcg
     3001 taacgggttt tgtgcatatc ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt
     3061 tcgggaccga aataggcggt gtcgccgatg ccggaagatt tcaaataggc ttcggctcgg
     3121 cgggcgatgg agcgcgggtc gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg
     3181 caagtcaaca caacagtcgc atcatcataa aaaggatcga cgaaggctgt agacgcatcg
     3241 gggcgcaact gcatatcgga agcctgaatg cctttccagc cgccgataga cgaaccgtca
     3301 aacgcctgac cgttttcaaa ccactcttcg gggtcttcca acacgatgcg cgcaggcacg
     3361 gtaaagtggt gctgcttgcc tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg
     3421 ctttcttcaa tcaattttac ggcgttttta atagacattt ttcagctcct ggaaaaggga
     3481 aatagcaaaa tatgcggaac gcggcggctt gacgcaaagt cggcatgatt ctaccacgtc
     3541 catgccgtct gaaactcgcc ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg
     3601 caagattgtc aacaatatgc ttctgtttgt ctgttcaaaa gcggaatacc cgcccttttg
     3661 tccgaatgcc gacaaaacag atgccgtctg aaagcagttt agacggcatc gttcccatct
     3721 tcgcgccgcc gttataaaat gaccgccccg ccttgatgga atatgactat gcacgccctc
     3781 ccccgctacg ccgtttttgg caaccccgtc gcccacagca agtcgccgca gattcatcaa
     3841 caatttgccc ttcaggaagg cgttgacatt gaatacgaac gcatttgcgc cgacatcggc
     3901 ggtttcgcgc aggcggtttc gacatttttt gaaacaggcg gttgcggggc aaacgttacc
     3961 gtaccgttca agcaggaagc gtttcatctg gcggacgagc attctgaacg cgcattggct
     4021 gccggcgcgg tcaacacgct gatttttctg aaaaacggaa aactgcgcgg cgacaatacc
     4081 gacggtatcg gtttggccaa cgacatcacg caggtcaaaa acattgccat cgaaggcaaa
     4141 accatcttgc ttttgggcgc gggcggcgcg gtgcgcggcg tgattcctgt tttgaaagaa
     4201 caccgtcctg cccgtatcgt cattgccaac cgcacccacg ccaaagccga agaattggcg
     4261 cggcttttcg gcattgaagc cgtcccgatg gcggatgtga acggcggttt tgatatcatc
     4321 atcaacggca cgtccggcgg cttgagcggt cagcttcctg ccgtcagtcc tgaaattttc
     4381 ctcggctgcc gccttgccta cgatatggtt tacggcgacg cggcgcagga gtttttgaac
     4441 tttgcccaaa gcaacggtgc ggccgaagtt tcagacggac tgggtatgct ggtcggtcaa
     4501 gcggcggctt cctacgccct ctggcgcgga tttacgcccg atatccgccc tgttatcgaa
     4561 tacatgaaag ccatgtaata tgttccgcat cgtcaaatgg ctgattgccc tgcccgtcgg
     4621 catctttatc tttttcaatg cctatgtgta cggcaacatc attacctacc gcgccgtcgc
     4681 gccccatcag actgccttta tgtcgatgcg gatgaagcag tttgaacagg aaggtcgcga
     4741 tgtcgcactg gattaccgct ggatgcccta caaccgcatc tcggtcaacc tgaaaaaagc
     4801 cctgattgct tccgaagatg cccgtttcgc cgggcacggc ggcttcgatt ggggcggcat
     4861 tcaaaacgcc atcaggcgca accggaacag cggcaaagtg aaggcgggcg gctcgaccat
     4921 cagccagcag cttgccaaaa acctcttcct caacgaaagc cgcagctata tccgcaaagg
     4981 cgaagaagcg gcgattaccg cgatgatgga agccgttacc gacaaagaca ggatttttga
     5041 actgtattta aactcaatcg aatggcacta cggcgttttc ggcgcggaag ccgcgtcccg
     5101 gtatttttat caaatacccg ccgccaagct gaccaaacag caggcggcaa aactgacggc
     5161 gcgcgtcccc gccccgctct actacgccga ccatccgaaa agcaaacggc tccgcaacaa
     5221 aaccaatatc gtgctcagac gcatgggttc ggcagagttg cctgaaagcg acacggactg
     5281 attgtttcag atatggaaat gccgtctgaa ctgcggttcg gacggcatat gttttctggt
     5341 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     5401 cacggaatcg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     5461 cgagccaacg ctgtaccggt ttaaatttaa tccactatac ctgcccgaca gccgcaaaga
     5521 ccgtatcata catagcaggc attgcggtgc gggattactg ccgcaaacgg caggcaaaaa
     5581 aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg gaatcgaacc gccgacacac
     5641 ggattttcaa tccgttgctc taccaactga gctatctggc ctttgtctgt ctcgttaaga
     5701 gatgcgtatt aaaccaaatt acgggacgac atgcaagcat atcgggcaat attctttgaa
     5761 aacaggttta agtcaatgtt ttaaatttga attaatttat gtaaaaacaa cataaatccg
     5821 tatttttgcc gcctgcccga cgatattagg tcgcccgtct gaaaaatatt ttcaatattt
     5881 gaagttctta cccagataaa cagaacgaac ctgttcgttt ccgaccaaat catcaggttt
     5941 tcccgatgcc agcaccgcac cgtctgaaat gatgtaggcg cggtcgcaga tgctgagggt
     6001 ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg cgcgacttga gaaatccgat
     6061 gattttttgg atgtcgatga cggcgatggg atcaacaccg gcaaaaggct catccagcaa
     6121 aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg acgcgccgcc gttcgccgcc
     6181 ggacagcgac ggcgcggggc tgcggcgcaa gtgtccgata ttaaggtcgg cgagcagttt
     6241 ttcgatttcc ctgtcgattt gatttttatc tttggttctg atttccaaga tggcgcggat
     6301 gttttgttcg acggtcattt tgcggaatat cgaggcttcc tgcggcaggt agccgacacc
     6361 gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct tgtccgtcta gggttacgct
     6421 gcctgcgtcg gcggcaatga gtccgactat catgtagaag ctggtggttt tacccgcgcc
     6481 gttgggcccg agcaggccga cgacttctcc gctttcgatt tcgagggaga agcttttaac
     6541 gacttggcgt tttttgaaac ttttttgcag gttttgaaca acaaggcggc tgacgtttgc
     6601 gctcataaat atctcttcaa tatcttcggt cggcaaatgc cgtccgaacc aggtttatgt
     6661 ttcagacggc attttgggat tattcggatt tttgcgtact cgaaggctgg ataacgacgc
     6721 tgaccctgcc ggatttggaa gcggattttg cgccggattt tgtgctgccg ctgatggtat
     6781 agacttcggt tttggtgttg tatgtaatca ccgcaccttc ggcgacatcg ccgccgcgct
     6841 gtactttggc attaccggtt aagactacgg tgctgcctgc agatgaataa gcaacgttgt
     6901 ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt ctggctgaag cggactggcg
     6961 aaccttccgc cctcacggat tcgccgcctt tgccgccgcg tgtaacattg acgcgggcgg
     7021 cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc gctgaatgtg gtgctttggt
     7081 tggcttgatc gagcgaacct tggtcggcct caatctgaat aggctgcctg ctgtcgcttt
     7141 gaagggcaaa agcgggggac accgaaaaaa atgctattaa aacaaatagc ttacatatct
     7201 tttgtatcat aaatcgtggc tttcacttta gatgagaagt tcaacatgcc tgttttgtgg
     7261 tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat attggaaact gacaggcgta
     7321 tcggtttggg catattgaga ttcggtatcg acgtgcagct tttcggcttc aactttaccc
     7381 gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt tgtttttaaa aagaacctgt
     7441 ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt cgtacaacaa cctgccttct
     7501 tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc tgctttcggg aaactgtttc
     7561 gcgcccttcg aactcaaatg ttctttcaag tatccctgtt cgtcaaaccg cctgccatcc
     7621 aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc tgacttcttc aatctcgact
     7681 tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg caaccgccaa tatcaatggg
     7741 aacgcaattc cgtaccgcca tcttactttc atttgatgta ctcgttcaaa gccgcgccca
     7801 aagtcccttg cgcctgcata atcaggtcgc acacttcgcg caccgcgccc gcaccgccgg
     7861 ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca atgcgcgtcg gggacggcaa
     7921 ccggcaatcc gcagcgtacc ataacgggca aatcgaccac gtcgtcgccg acaaaggcac
     7981 actcggcttc ttccacgccg acctgagcgc gcagctcttc atacgcggcg cgtttgtccg
     8041 aaatgccttt gaagtagtaa tttatgccca actgtttgac gcggatgccg acggagggcg
     8101 cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc ctgaagcatt ttcagaccgt
     8161 gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt atcgcggata aagatgcgcc
     8221 cgtcggtcaa aacgccgtcc acatccagga tcagcagttt gattttggcg gcgcgcgcct
     8281 gtaattcggg agaaattgcc tgcatgggat ttccttgttt tcagacggca tatcttacca
     8341 caaatgccgt ctgaaacggc tgcaacgcct tatatagtgg attaacaaaa atcaggacaa
     8401 ggcgacgaag ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac
     8461 cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc
     8521 cactatacaa tccgcgccgc caaaaggtcg tgcatattca gcgcgccgat cagcacgcca
     8581 tctgcatcgg taaccagaag cccgttcaca tggtttgcct gcatgacttt cagggcttcg
     8641 gtggcgagac gttcggcgga gatggtttta gggtgtgtat gcatgacttc gtctatcgaa
     8701 agaccggtaa aattgtcgca ttcttgaaac aggcggcgca aatcgccgtc ggtgaatacg
     8761 cctttcagac ggccttgccc gtccgttacc gccaacatgc ccagcccttt ttcactcatg
     8821 ctgacgatgg cttctttcaa gggcgtgccg agtcggacgg caggcaggcc gccgcctttg
     8881 tgcataatgt cggcaacgcg caaaagtagg cgtttgccga ggctgccggc agggtggctc
     8941 aaggcgaagt cgtcgggcgt gaacgcgcgg gcgcgcagca ggacaaccgc caacgcatcg
     9001 cccaaagcca taacggcggt ggtgctggtg gtcggggcaa gccccagcgg gcaggcttct
     9061 ttggaaaccg acgccgtgat gtggatgtcg gcatggcgcg ccatggttga atcggggcgg
     9121 gcggtgatgc agacaagcgt gatatctttg cgtttgagcg cggggatgat ggcggcgatt
     9181 tcgtcgcttt caccggaatt ggaaatcgcg acgaccacgt cgttgtccac aatcatgccc
     9241 aaatcgccgt gtgccgcttc cgcagggtgg acgaaaaacg cgggcgtgcc ggtcgaggcc
     9301 atggttgccg ccattttgcg cccgatatgt cccgacttgc ccatgcccgt gataacgacc
     9361 ctgcctttgc agtgcaacaa cgcgtccgcc gcaagggcga agttttcgtc caaatccgcc
     9421 gcaatttcgc gcaagccttc cgcttcggtg tgcaacactt cgcgtgccca atcaagatat
     9481 ttttcgtttc ctgccatcgc catccgcctt tgctgtaata aaactaccca aaatttccga
     9541 atcggggtag cataagcctt tgaaacatac acatccatcc aaaggaatca gaaatgacta
     9601 ttttatcgga cgttaaagca ttaggacaac aaatctggct ggacaacctt tcccgctcgc
     9661 tcgtgcaaag cggcgaattg gcgcaaatgt tgaaacaagg cgtgtgcggc gtaacttcca
     9721 atcccgccat ctttcaaaaa gccttcgccg gcgatgcgct ttacgccgac gaggttgccg
     9781 ccctcaagca gcaagacctg acccccaaac aacgctacga aacgatggcg gttgccgatg
     9841 tgcgtgccgc ctgcggcgtc tgtcttgccg aacacgaatc caccggcggc aaaaccggct
     9901 tcgtcagcct cgaagtttcg cccgaattgt ccaaagacgc gcaaggcacg gttgaagaag
     9961 cgcgccgcct ctacgccgcc atcgggtgca aaaacgcgat gattaaagtg cctgccaccg
    10021 acgcaggcat cgatgcgctc gaaaccctcg tttcggacgg catcagcgtg aacctgaccc
    10081 tgctgttctc acgcgcccaa accctcaaag cctacgccgc ctacgcgcgc ggcattgcca
    10141 aacgcttggc agccggacaa agcgttgccc atatccacgt cgtcgccagc ttcttcatct
    10201 cgcgtgtgga cagtgcgctg gacacaacac tgcccgaccg gctcaaaggc aaaatcgcca
    10261 tcgcccttgc caaagccgcc tatcaagatt gggaacaata tttcaccgcc cccgaatttg
    10321 ccgcactgga agcccaaggc gcaaaccgcg tgcagctttt atgggcatct accggcgtga
    10381 aaaacccagc ctatcccgac acgctctacg ttgacagcct gatcggcgcg cacaccgtca
    10441 acaccgttcc cgatgccacg ctcaaagcct ttatcgacca cggcacagcc aaagccacgc
    10501 tgaccgaagg cgtagaagaa gcacaagcgc agctcgccga aaccgccgcg ctcggcatcg
    10561 atgtcgaaac cttggcaacg cgtttgcagg aagacggttt gaaacagttt gaagaagcgt
    10621 ttgaaaaact gctcgcaccg ttggtttaaa cttttcatcc ccgatgccgt ctgaaaggct
    10681 ttcagacggc atcgcccttt tgaccggaca cagtgggttc accctaaaaa tattaatcaa
    10741 caagaagtta atgcacgaca ttacccacca acacaaaagg aaatagaatc atggcaaaag
    10801 cactcgaaat catttcaccc aatgaaattt attcagatct aatttttaag aatccggtac
    10861 ctccccatac tatatataca gaattgatga aactgattgg tagaaatgca agtaatgaga
    10921 gaatcgcaga tagagccttc gattttttct cgtccggcct tatggatggt tcagctaccg
    10981 aagagcaata taatgctcta tatgatttaa ccttgcttga agagcccggg atggaactga
    11041 ataaagatga gataatggct ttgattcata gtcttaaata aaggggctgt accggatatg
    11101 tacacaggac gtttcgcccc cagcccgacc gggctgctcc acatcggctc gctgctgacc
    11161 gccgccgctt cctatgccga tgcacgctca aacggcggca aatggctggt ccgcatggaa
    11221 gacctcgatc cgccgcgcga aatgccgggg gcggcaagcc atatcctgca cacgcttgag
    11281 gcattcggat tcgagtggga cggagaagtc gcctatcaga gccaccgtta cgccctgtat
    11341 gaagaaaccc tatgccgtct gcaaaccgcc ggactggtct atccctgcca ttgcagccgc
    11401 aaagactggc aggccggggc aaggcggggc gcagacggat tcgtctataa cggacgttgc
    11461 cgccacccct gccaacgccc tgcaccgcaa ggcaaacagc cggcgtggcg catccgcgtc
    11521 cccgaccgca ttatcggttt ttcagacggc atcgtcggcg gttacgccca aaacctcgcc
    11581 cgcgacatcg gcgatttcgt cctgcttcgt gcagacggtt actgggcata ccaactcgcc
    11641 gtcgttgccg acgatgccga acagggcgtt acccacatcg tccgcggaca agacctgctt
    11701 gtttctacgc cgcgccaaat ctatttgcag cagtgtttgg acgttccgac accgcaatat
    11761 gcccacctgc cgctgctgac caacgcacaa gggcagaaat ggtcgaaaca gacgctcgca
    11821 cccgcattgg atttaaaccg ccgcgaacaa ctcctccgcc aagtgttccg ttacctcaac
    11881 ctgcccgaag caccggaagc cgaccgccct gccgaactgc tcgactgggc ggtggcacac
    11941 tgggatatgg gcaaagtgcc gaaacacgcc attaccgccc cctaaacacc gcccgacaaa
    12001 cgcacaaatg ccgtctgaaa ccttcagacg gcatttcata cacacagccc cgccctattc
    12061 gcgcatattt gcccgttcga cttctttcca cgcttcgaga atcaggccgc cgtcgttgtc
    12121 tttcaagagc gttgcatcgg aaagcatacg ccgccaagtg cgggcatttt tcagaccgtg
    12181 catcagcccg aggctgtggc ggacgatgtg gcgcaagatt gtgccgcgtc cggcttggat
    12241 ttgggcttga ctgtatgtgt agagacgctg caccaaatcg gcgtattcaa tcgggctgcg
    12301 ggtatcgccg taaaacagcc tgtcccattc gcgcatcacc atcgggttgt ggtacgcctc
    12361 gcgcccgacc atcacgccgt caacgtgttg caggtgttgc gcgatttcct cattggtcgt
    12421 gatgccgccg ttgatgatga tttccagccc gggaaactct tgcttgaggc ggtaaacata
    12481 atcgtatttc aagggcggaa cgtcgcggtt ttctttgggt gaaagaccgt ccagccacgc
    12541 gttacgtgcg tggacgataa aggttttgca ggcggttttg tcgcgcagcg tgccgacgaa
    12601 atcggcaacg gtttggtatt cggtctgcct gtccacaccg atgcggtgtt tgacggtaac
    12661 gggaatcttg accgcatcct gcatggcgtt gaggcagtcg gcaaccagcc cgacttcgtt
    12721 catcagacac gcgccgaacg cgcctttctg gacgcgcgga ctggggcagc cgcagttgag
    12781 gttgacctcg ttgtagccgt attcttcggc ggctttggcg gctttcgcca aatcggacgg
    12841 gtcgctgccg cccagttgca gggcgacggg ctgctcgcct tcgttaaaca tcaaaaagcg
    12901 gtctttgtcg ccgtaaacaa tcgcgccggc gttgaccatt tcgctgtaca gccaagtatt
    12961 gcgggtaatc tggcgcgcca tataacggta gtgcctgtcc gtccagtcca acatcggggc
    13021 gacggacagg cggcggggag gaagggtttg cgtgttgtcg ttcattgtcg atatttcacc
    13081 attgcggatt caatttcaat ccggaacaac cttcattttt ggggaaatcc gcgtcatgaa
    13141 agagtccagc agtatggtta ccggcccgtc gttgcagagc gacacctgca tatgcgtgcg
    13201 gaaacgccct gtttcgacat gaattccgtg tccgcgcaac agttccgccg ttcgcaggta
    13261 aagctgctgc gcctgttctg caggtgcggc ttgcgaaaac gaaggccgcc gcccgcttgt
    13321 cgcgtcggca taaagtgtaa actgcgacac cagcagcacc gcgccgccga catctttcaa
    13381 agacaggttc agcttgcccg cttcgtcttc aaacacgcgc aaatgggcga ttttgtcggc
    13441 gatatagtgc gcatcttttt ctgtgtcgct atgcgttacg ccgagtaaca cgacaaaccc
    13501 gccgtcgatt ttgccacagg tttccgtgcc ggcttcggac acgacatcca cctttgcacc
    13561 taccgttttc tgtatgaccg cacgcataag ccgtcctcct tacctgaacg tgcaatgccg
    13621 tctgaaaagg cttcagacgg catcggtatt ggaaatgcca ttacggtttg acaccgtttt
    13681 cttccaaaat ggcatcgatt ttcaagcggg ctttttcctg tctcaaacct tgttccaact
    13741 ggtttctgac caactcgaaa ggctgcgcgt cggggttttt cccgacctcg ctgagtttga
    13801 acagataata gcgttcgccc aatttgaccg gatcgcgggt aacgtcgccc cgattcatcg
    13861 ctgcaaactg cgaagccagc ggctcgggaa gctgctgcgc cataatgaaa ccgtcaaaag
    13921 cctgctcgtc gttcggataa cgcttcatca gcccttcaaa agacagccct ttgagcagga
    13981 gctgatgcgc ctgacgcgcc tcctcttcgg ttgcgaagct gacctgctgc aatttgatca
    14041 tgcggatttg ccgctcataa aactgacgca gtgcgctttc ggaaaccgtt tccgaacgtt
    14101 ccagaaaacg gacgtactcc tcggcataaa aagacgcttc ggcgatttta aagcggtttt
    14161 ggacatcctt atccttatcc aaaccttcct tcaatgccct gtttttcaaa acttccaaag
    14221 tttgcagccg acggacggca tcgtttcgga ttgcctgccc gtccggtttt tgggactgct
    14281 ccgcatgccg gtctgcctgc tgcatgatct gcgccaccag cgtatccacc aaagccgggt
    14341 cgatttcggg tgctttggct gccgcaaaac ctgccaacat tgcagcaata actgcggcag
    14401 cggttttttt ctgtttcatg gtattccctg tcctgcgcgg cggcaaaacg cctgtctgaa
    14461 cgtcttgccg ccatatcccg ttttcagaat tattttgcag gtttgatgtt tgccttgccc
    14521 aacagtgcac cgacggcacg gtcaatccgt tccgcctgaa ggttgccggc aatctgtcct
    14581 ttcatttcat caaaagaagg cacttttacc tcgcggctgt cgttgacata ataaacgccg
    14641 tagaaatcgc cgtttttcag cggcgttgcc gtaaattcgc cttttttcaa gtccttaatt
    14701 gcctgataaa gcggcggaac accctgttcc aaatctttca gcggcacata tccgaccggc
    14761 gcgccggtct gtttggtacg gtcgttgagg gaatattgtt tcaagacggc atcgaaacct
    14821 tttttcgcct tcaagtcggc aaccgctttt tttgcatttt cttccttgtc ggtcaggatt
    14881 tcgcccaact ggacttcctg cgtacctttg taaaaaccgc tgatattgtc atatgcggct
    14941 tttacttcct gctcggaaac cggttgggtt ttggcgatat gcagcgcgta tgcctcgccg
    15001 ttcaagccat attttaccgc ctgccaaacg gttttgaagg acggtttctt gtcgtcgccc
    15061 gactttttcg cttcggcacg caatttggca agcgcatttt taaactctgc cgaccggtcg
    15121 agtttcagac gtttcacttc ctgtgcgacc acggtgttga ccacttcgtt ttccagcagg
    15181 gattggcgca gttgcggcgt gtcttcggca cggctgtttt ccgcacggaa tgcggcaacc
    15241 tgcgcatcga tgacggaact gtcgattttc tgaccgttga cggttgccag cgtttgggca
    15301 aacaggctgc cggaacaggc aagcagtgca acggaagtca ggatttttgc tttcataatc
    15361 ttctctcttt aaattttaaa ttgaaatgtt cgggcagact tcattcagtc cgccgtatgg
    15421 aaatactcgt caggggtagc cgcccggatg ccgagcgcgt gaatcatgcc gcctgaaaac
    15481 aaatctttga gcagcgattt gaccgttttc tggcggttca ggcggcttac gccttcaaaa
    15541 cggccgctga cgaccaaaac ggcataatgt ccgccgccgg tattgcccgc gtgtcctttg
    15601 tgcagatggc tctcatcgcc gatttccaac accagcggat cgagcgtctg caggcgttcg
    15661 cggatcaaat cgacggccgg cattatttga acaccgcttt aaacggcttg atcagcactt
    15721 cgctgtacac gcctgcatga acatagggat cgtcttccgc ccacgcctct gccgcatcca
    15781 aagactcgaa ctgcgccaca atcaaactgc ccgaaacgcg gttggagtcc tccggcaaag
    15841 gattcgggcc tgccgtcaac agccggcctt ccgacttcag cgtctccagc cgtttgaggt
    15901 gttcgggacg tgccgccata cgcgcttcat acacattctc cccgtctgtt gccagcaaca
    15961 taaaatattc cacagtcaat cctccttttt cagacaggta ctcagataaa taccctgaat
    16021 aataaagaaa acaagcatca gtgcagtcga gccgaacatt ttatagttga cccattgcgc
    16081 ttcaaaccta gtaaacacaa accagttggc aatacccata aaaatcagaa aaccgaccca
    16141 catatacgtc aatttcgccc atacggcatc tgaaagctga atctccctgc cgatactcgc
    16201 cttcaagccg tttttaccgg cgaggtggct gccccacagg aacagcgcgc ccagccaaaa
    16261 caaaacgctc ggcttccaca taatgaagcg gctgtcgccc aaaacaatgg ttgcgccgcc
    16321 gaataccaca atcagcacca atccgaccca ctgcatcgta tccagctttt tatatttcca
    16381 atacagaaaa gccgcctgaa ccacaccggc aaccaatgcg accgccgttg cggcaatcat
    16441 gtttttggta acggtatagg tggcgaaaaa caggatgacg gacaaaaggt cgctgacgaa
    16501 tttcatatcc aaatgagaaa gcaaatcaaa taaacgctaa tcatacaggc agaaccctgt
    16561 ttttgcacct gcaaaaccaa caaattttgt tttgattgat gcaaatatgc acagatgttt
    16621 ttgaaaaaag atggagatat gtcataattt gtaaaaacgg cgaatgattg ccgtttaaaa
    16681 tgtggcggcg gtcggtacat tcacatatta aatgcccgcc ggccgactgc cccgccgatg
    16741 cccgatgatg acgaaattta cagactgtac gcggtcaaac cgtattcagc cgccaaccca
    16801 caggggatac atcttgaaaa acaacagaca aatcaaactg attgccgcct ccgtcgcagt
    16861 tgccgcatcc tttcaggcac atgctggact gggcggactg aatatccagt ccaaccttga
    16921 cgaacccttt tccggcagca ttaccgtaac cggcgaagaa gccaaagccc tgctaggcgg
    16981 cggcagcgtt accgtttccg aaaaaggcct gaccgccaaa gtccacaagt tgggcgacaa
    17041 agccgtcatt gccgtttctt ccgaacaggc agtccgcgat cccgtcctgg tgttccgcat
    17101 cggcgcaggc gcacaggtac gcgaatacac cgccatcctc gatcctgtcg gctactcgcc
    17161 caaaaccaaa tctgcacttt cagacggcaa gacacaccgc aaaaccgctc cgacagcaga
    17221 gtcccaagaa aatcaaaacg ccaaagccct ccgcaaaacc gataaaaaag acagcgcgaa
    17281 cgcagccgtc aaaccggcgt acaacggcaa aacccatacc gtccgcaaag gcgaaacggt
    17341 caaacagatt gccgccgcca tccgcccgaa acacctgacg ctcgaacagg ttgccgatgc
    17401 gctgctgaag gcaaacccaa atgtttccgc acacggcaga ctgcgtgcgg gcagcgtgct
    17461 tcacattccg aatctgaaca ggatcaaagc ggaacaaccc aaaccgcaaa cggcgaaacc
    17521 caaagccgaa accgcatcca tgccgtccga accgtccaaa caggcaacgg tagagaaacc
    17581 ggttgaaaaa cctgaagcaa aagttgccgc gcccgaagca aaagcggaaa aaccggccgt
    17641 tcgacccgaa cctgtacccg ctgcaaatac tgccgcatcg gaaaccgctg ccgaatccgc
    17701 cccccaagaa gccgccgctt ctgccatcga cacgccgacc gacgaaaccg gtaacgccgt
    17761 ttccgaacct gtcgaacagg tttctgccga agaagaaacc gaaagcggac tgtttgacgg
    17821 tctgttcggc ggttcgtaca ccttgctgct tgccggcgga ggcgcggcat tgatcgccct
    17881 gctgctgctt ttgcgccttg cccaatccaa acgcgcgcgc cgtaccgaag aatccgtccc
    17941 tgaggaagag cctgaccttg acgacgcggc agacgacggc atagaaatca cctttgccga
    18001 agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac gatgtaaacg acacacttgc
    18061 cttagatggg gaatctgaag aagagttatc ggcaaaacaa acgttcgatg tcgaaaccga
    18121 tacgccttcc aaccgcatcg acttggattt cgacagcctg gcagccgcgc aaaacggcat
    18181 tttatccggc gcacttacgc aggatgaaga aacccaaaaa cgcgcggatg ccgattggaa
    18241 cgccatcgaa tccacagaca gcgtgtacga gcccgagacc ttcaacccgt acaaccctgt
    18301 cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc caaactgccg aaaacaaacc
    18361 ggaaaccgtc gataccgatt tctccgacaa cctgccctca aacaaccata tcggcacaga
    18421 agaaacagct tccgcaaaac ctgcctcacc ctccggactg gcaggcttcc tgaaggcttc
    18481 ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc caaacaccgg aagagttgca
    18541 cgatttcctg aaagtgtacg aaaccgatgc cgtcgcggaa actgcgcctg aaacgcccga
    18601 tttcaacgcc gccgcagacg atttgtccgc attgcttcaa cctgccgaag caccgtccgt
    18661 tgaggaaaat gtaacggaaa ccgttgccga aacacccgat ttcaacgcca ccgcagacga
    18721 tttgtccgca ttacttcaac cttctgaagt acctgccgtt gaggaaaatg cagcggaaat
    18781 cgttgccgat gatttgtccg cactgttgca acctgctgaa gcaccggccg ttgaggaaaa
    18841 tgtaacgaaa accgttgccg aaatacctga tttcaacgcc accgcagacg atttgtccgc
    18901 attacttcaa ccttctgaag tacctgccgt tgaggaaaat gcagcggaaa ccgttgccga
    18961 tgatttgtcc gcactgttgc aacctgctga agcaccggcc gttgaggaaa atgcagcgga
    19021 aatcactttg gaaacgcctg attccaacac ctctgaagca gacgctttgc ccgacttcct
    19081 gaaagacggc gaggaggaaa cggtagattg gagcatctac ctctcggaag aaaatatccc
    19141 aaataatgca gataccagtt tcccttcgga atctgtaggt tctgacgcgc cttccgaagc
    19201 gaaatacgac cttgccgaaa tgtatctcga aatcggcgac cgcgatgccg ctgccgagac
    19261 agtgcagaaa ttgctggaag aagcggaagg cgacgtactc aaacgtgccc aagcattggc
    19321 gcaggaattg ggtatttgat tcccaactgc cctttcgcag atcaaggatg ccgtttcaga
    19381 cggcatcttt ttttgcctta tcggtgtaac ggataaagtt tgaaccggca caggctcaaa
    19441 cagcaggccg acggcaacaa aatgccgtct gaaaccccta aaggcttcag acggcattgg
    19501 cggcggcggt atcaggcagt ttgataggca accgaatcgt caccgctttt cttttgaatg
    19561 aactcgattt tgtatccgtc ggggtcttcg acgaaggcta tcacggttgt gccgtgtttc
    19621 atcgggccgg cttcgcggac gacgtttccg ccctgccgct tcacacgttc gcaggcttcg
    19681 taggcatcgt ccacttcaac cgcgatgtgt ccgtaggcgt tgcccaagtc gtatcgttcc
    19741 gtatcccagt tgtgcgtcag ttccaaaacc gtgctgtcgg tttcgtcgcc gtaaccgacg
    19801 aaggcaaggg taaatctgcc ttcgggataa tcttttcggc ggagcagttt catacccaaa
    19861 acgttttggt agaaatcgag ggatttttcg agattgccca cgcggagcat agtatggagt
    19921 aagcgcattt ttgtgttcct ctcggtagtg gttaaacttc gattttgttt ggcgtaaacg
    19981 tctgccattt gttgcaggca ggacaatgcc agaaaaagac ttgtgatttg aagtggcagt
    20041 ttcggcaccg gtacatcacg ctgcgctgta gctgccgtcc gataaccgaa cgcatcatat
    20101 cggcatcggc tttccaagcc ggatccaaat cgctgagttt caaaccaagc aggcggtaca
    20161 cgccgttgag gtcgggcttg cggcggacaa gctcgacggc ggtttgcgcg gcttctttct
    20221 cgcacttaag cagcagggat ttctcgtaca cgacattgat caggtcaagt tcgggaaacg
    20281 tctgcatata tcctgtcaga cggttcaagc cttcttcagg ttttccctgc gcggcatagg
    20341 cttcgtaaag cttctcgccg accatgctca agtatgcatg gttttgctgc tcgatggcgg
    20401 cataggcttc gacggcggca gggaaattgc cttgtcggtg ttcgatgtcg cccaaaatca
    20461 tgttggcacg ggtgcatttt ttgttggctt cgagtgcctt gccgacattg aaacgcgcga
    20521 catcgaaatt ggacttgaac agcgcggctt gggcaagttc gcaataaaac tgggcgattt
    20581 caaactgata ggtctgatcg tcatggctga gcagccgggc ggtttcaacc gctttttccc
    20641 aatccctgtc ctgttggtag atattgagca ggtgctgtct ggcttcacgc gccattttac
    20701 cgtcttgcag ccccaaaaaa atctgttcgg cacgatcgac caaccccgca ctttggtagt
    20761 tttgcgccaa ttcaaacagg acgcgcgcgc gcttttcgcc gaccgtatcg ggagaatcga
    20821 gcattgtccg gtgtatgttg atggctttgt cgttttcgcc acgctgacgg taaagcttgc
    20881 ctagggtgag gtttaggtcg tatgactgag gctgctgatc aatgacctct gccaattccc
    20941 tggcggcacg tccgctgttg cggtcaacca aggcatccag acttttataa aatcccgacg
    21001 gtatgctttt tgcctgcttt aatacagtct tcatatccac acgggcggca aaccagccca
    21061 tcgcgaagaa aacgggcaaa aggataatcg gcagcaggat aatccacaat tcattgtcca
    21121 tatcggcttt ctcaaggctg tttggcagat tcgggcgcat tttgcgccgg tggtgcggtc
    21181 agctctttcc ccgtcaaacg cgcatttttc tttacttcgg cacgcaacct gccgttctcg
    21241 ccacgtaacg acaacaaccg tccgaacaag gcaaacattc cgaacacgat gccgacgaca
    21301 aacgcgccga acaatacgac aatcagcggc aaatcgactt tctgtccggg caggtaggag
    21361 aaggtaacgg catccgtatt aatgacggca agcagcagga agaacagcag gataatgatt
    21421 ttgacgacgg tatagataag tttcatgttt gctccgtaaa taaaaacgcc gcccggacag
    21481 cttcgcactc tgaaccaaga ttgcgctagt ttaaacgatt tgcacggttt cggataggat
    21541 gcggcagcgt gttcggacga catacggagt atgcgattgc cgacaatttt accaaataca
    21601 ccgttccttt ccatttgaaa aataacggat tggacaccgc atcgacagaa aaacccgccg
    21661 cgcacttgtc aaaaccctgt ttgcaggcgt atctttacaa tcttcaaatt caaaccgttc
    21721 attgaaacat atcagaataa gaaaggcttt acatcatgag cagacccgta cccgccgtat
    21781 tcggcagcgt ttttcacagt caaatgcccg tcctcgccta ccgcgaaggc aaatggcagc
    21841 cgaccgaatg gcaatcttcc caagacctct ccctcgcacc gggtgcgcac gccctgcact
    21901 acggcagcga atgtttcgag ggactgaaag ccttccgtca ggcagacggc aaaatcgtac
    21961 tgttccgtcc gactgccaac atcgcgcgta tgcggcaaag tgcggacatt ttgcacctgc
    22021 cgcgccccga aacccaagcc tatctcgacg cgctaatcga attggtcaaa cgcgccgccg
    22081 atgaaattcc cgatgcgcct gccgccctgt acctgcgtcc gaccttaatc ggtaccgatc
    22141 ccgttatcgg caaggccggt tctccttccg aaaccgccct gctgtatatt ttggcttccc
    22201 ccgtcggcga ctatttcaaa gtcggatcgc ccgtcaaaat tttggtggaa accgaacaca
    22261 tccgctgcgc cccgcatatg ggccgcgtca aatgcggcgg caactacgct tccgccatgc
    22321 actgggtgct gaaggcgaaa gccgaatacg gcgcaaacca agtcctcttc tgcccgaacg
    22381 gcgacgtgca ggaaaccggc gcgtccaact ttatcctgat taacggcgat gaaatcatta
    22441 ccaaaccgct gaccgacgaa tttttgcacg gcgtaacccg cgattccgta ctgacggttg
    22501 ccaaagattt gggctatacc gtcagcgaac gcaatttcac ggttgacgaa ctcaaagctg
    22561 cggtggaaaa cggtgcggag gcgattctga ccggtacggc agccgtcatc tcgcccgtta
    22621 cttccttcgt catcggcggc aaagaaatcg aagtgaaaag ccaagaacgc ggctatgcca
    22681 tccgtaaggc gattaccgac atccagtatg gtttggcgga agacaaatac ggctggctgg
    22741 ttgaagtgtg ctgatgcttt aaataaaaaa tgccgtctga aacccgtttg gcgtttcaga
    22801 cggcattttc gcatccgaac cgtttccgct gcacctgcag caagtcggca caaaggcaat
    22861 cggttaaaac aagcgtccgc atttctcatc ccgcctgccg caagtcgggt atttctctgg
    22921 aaatacgctt cagacggcaa aacgctgccc gaaaccgata cgcggcacgg acggcgcaca
    22981 gatttgaaaa cagcggatta tccctcggtg ctcaaggcat tgatgctgta accgccgtca
    23041 acgtaagtaa tttcgccggt aataccggac gacaggtcgg acagcaggaa ggcggcggta
    23101 ttgccgactt cttcgatggt aacgttgcgg cggaggggat tgtgggcggc gacgtgtccc
    23161 aagagtttgc cgaaatcggc gatgccggag gcggcaagcg ttttgatcgg gccggcggaa
    23221 atgccgttgc agcggatgcc ttctttgccc aaacaggcgg cggtaaagcg gatgcctgcc
    23281 tcaaggctgg cttttgccat acccatcacg ttgtagttcg gaatcgcgcg caccgcgccc
    23341 aagtagctca gggcgacgat ggcggagttt ctgccgcgca tcatcggacg ggcggctttt
    23401 gccagcgcag gcaggctgta tgcggaaatt tcgtgtgcgg tgttgaacgc ttcgcggctg
    23461 atgctgtcga ggaaatcgcc gctcaaggct tctttcggcg cgaagccgat ggaatgcacc
    23521 aaaccgtcca agccgtccca atgcttgccc aagtcggcga acacttggtt gatttcgtca
    23581 tcgctggcga catcgcagcg gaatacgagt tcggaatcca attccgccgc cattttgcgg
    23641 acgcgctctt ccagtttgtc cacaacgtag gtaaacgcca gttccgcgcc ttgttcgcgg
    23701 caggctttgg cgatgccgta agcgatggaa cgctcggaaa tcatgccggt aatcagaatt
    23761 tttttgcctt gcagaaaacc cattttgtat ccttcaaata gtgttcggtt cgttctaaac
    23821 cgttgattat agcaaattgt ccctgtttct gtgttttcac gttgcagcgt gcaaacggca
    23881 atgccgtccg aagcggattt cagacggcat cggacgtttc aaatacggtt taaggcatca
    23941 gatgccgcgc aacaattcgt tcacgctggt tttcgcacgg gtttgcgcgt ccacacgttt
    24001 gacgatgacg gcgcagtaaa ggctgtggct gccgtctttg gaaggcatac tgccggacac
    24061 gacgaccgaa ccggccggta cgcggccttg gtagatttcg ccggtggtgc ggtcgaagat
    24121 tttggtggat tgaccgatga acacgcccat cgaaatcacg ctgccttctt cgacaatcac
    24181 gccctcaacg atttcagaac gcgcaccgat gaagcagttg tcttcaataa tggtcgggct
    24241 ggcttgcagg ggttcgagta cgccaccgat gccgacaccg ccactcaagt gcacgttttt
    24301 accgatttgc gcgcaagagc cgacagtcgc ccaagtatca accatcgcgc cttcgtcgac
    24361 gtatgcgccg atgttgacgt aagacggcat cagcacgaca tttttcgcca caaagctgcc
    24421 gcgtcgggca accgcacccg gaacggcgcg gaagcctgcg tttttgaact cgtcttcaga
    24481 ccagtcggca aacttggtcg gcactttgtc gaagtatttg ttcacgccgt cgttgaggac
    24541 ttcgttgtct tggatgcgga aggacagcaa cacggctttt ttcgcccatt cgttgacttt
    24601 ccactcgccc acgcccaagc gttcggcaac gcgcagtttg cctgaatcga gttggcggat
    24661 ggtttccaac acggcttctt taacttcggg agcaacagtg gacggggtga tgtccgcgcg
    24721 gttttcaaag gcggtttcga taatgttttg caaagacata atgtttcctt atgtgagatg
    24781 tttcgtggga cgcttgccgg tattttcaag cagtatgttc atttgaaatg aatgcgctat
    24841 tgtgccacgc aaacgcatat tcgtcagcaa tacgcgggcg gttgccgaac attggcgttt
    24901 cggaatcggg cgggcggcag gtattgcctt ttccgcaccc gaatcaatac agaacggcgg
    24961 cggtactttt atgccgcgcc gccgcctttc agacggcatt cacggtaaaa cgccatcagc
    25021 ccttcggtcg aggcatcgtg tacggacgtg ccgccttcca gttcgccgat gatggttttc
    25081 gccaactgtt tgccgtattc caccccccac tgatcgaagg gattgatgtc ccaaattaca
    25141 ccctgcacaa aggttttgtg ttcgtaagcc gccatcagca tacccagatt gtagggcgtg
    25201 aggcgttcga gcaggatgct gttgctgggg cggttgccgg ggaactcttt gtgcggcgcg
    25261 aggcgttcgc gttccgcttc gggcaaatct gccagttcgg cgcgtgcttc gtccaaggtt
    25321 ttgcccttca tcagggcttc cgcttgggca aaggcgttgg caacggtaaa acggctgcgt
    25381 ccgtcctcca cgccctgcgc cgtcatcggg acgataaagt cgcagggaat caggcgcgtg
    25441 ccttgatgaa gcagttggaa ataggcgtgc tggcagttga ccccttcacc gccgaacacg
    25501 atgccgcccg ttttgcacac ggcgggactg ccgtctgaag cgcggctttt gcccaaactc
    25561 tccatatcga gctggttcag ccacaccggc agcaggcgca ggttgtggct gtacggaacg
    25621 gcggtctgcc cgtccgcgtg ttggaaattg ttataccaca cggcaatcag tgccatcaga
    25681 acgggaatgt tgcggcgcgg cggcgtgtgg aaaaaatggc tgtccatcgc gtgcgccccc
    25741 gccaacaact cgcggaaacg cgccccgccg accgcaacca tcacgggcaa accgacgggc
    25801 gaccagacgg aatagcgtcc gcccacccag tcgtacatcg caaacacgcg ttccgccgcg
    25861 ataccaaaag ccgcagctgc cgcagtgtcg gcagacaccg cgcaaaaatg gcacgccgtt
    25921 tcggattccg agaaccccgc accgcgatac cacgccttga ctgcctgtgc attgagcagg
    25981 gtttccggtg ttttgaagga cttgctggca acgcaaaaca ctgtcgtttc ggggttcaga
    26041 cggcataaaa ccgcatccag gcaggcagga tcggcgttgg cggcaaaatg gacggcgata
    26101 tgccgtctga acggctcaag tgcctgcacg cacattgccg gcccgaggtc ggatccgcct
    26161 atgccgatgt ggacaaaatc cgtaatccgt tttccggttg tcccctgata cgaaccgtcg
    26221 tccaaactgt gtgcaaactt caacgcacga tttaactcgc ggcggatttc gggcaacacg
    26281 tccctgccgt ccgcataaac ggcacccgca ccgtcgggca ggcgcaaagc cgtatgcagc
    26341 gcggcacgcc cctcactgtt gttgaccttc gcacccgtcc gcaaagcacg cattttccct
    26401 tccaaatccg ccgcatcggc aagattgcag agcagttgca gcgtatcttc gcccaaacgg
    26461 tttttgctgt aatcgaacaa catcccgtcc aaacgctcgt gcatacgctc aaaacggtcg
    26521 ggttcggcag caaaacggtc gcgcaaaaga atgtggcgcg tatcctgata atggcgttcg
    26581 agcgcacacc atgcacgggt aaaagcatcc atctgttttc cttgattttt cagaaccgga
    26641 ttaaaatgta gtagaatgta gtttaacaaa cggcagcggc tttggcgaat ctccggaaca
    26701 ccgcccgccg cgtccgatag caccttattt ccatatggcg aaaatatagt gaattaacaa
    26761 aaatcaggac aaggcggcga gccgcaaaca gtacaaatag tacggaaccg attcacttgg
    26821 tgcttcagca ccttagagaa tcgttctctc ttgagctaag gcgagccaac gctgtaccgg
    26881 tttttgttaa ttcactatac cctccggccg gcatttgacc cgcaacaata tcctgcaaga
    26941 tttattgtgt acgcataaat gccggacagc cgcctaaata aggtataatc gcatccgatt
    27001 ctgtcccgtt tgccgcccgc aggcgggatg tcggcggttt tcatcttacc gggaacacgc
    27061 cggtactgtt ttttaacgat gtttcttaca ttttattcca attactttac ggggctagaa
    27121 tatggctaaa aacggaggat tttctttgtt cgcaaagaaa gaaaaacgct ttatctttga
    27181 aggcaggcat tccgcctccg acaaactggt caacggcgaa gtatccgcgt ttaccgaaga
    27241 agaggcgcgc aaaaaactgg caaaacgcgg catccgcccg ttgcagatta cccgtgtgaa
    27301 aacaagctcc aagcgcaaaa tcacacaaga agacatcacc gttttcaccc gccagctgtc
    27361 cacgatgatt aaagcgggcc tgccgctgat gcaggcattt gaaatcgtgg cgcgcggaca
    27421 cggcaacccg tctatgacgg aaatgctgat ggaaatccga ggcgaagtgg aacagggcag
    27481 ctcgttgagc cgcgcattct caaaccaccc aaaatatttc gaccgcttct actgcaatct
    27541 ggttgcggcg ggcgaaacgg gcggcgtatt ggaaagcctg ctggacaaat tggcaattta
    27601 caaagaaaaa acccaggcca tccgcaaaaa ggtaaaaacc gcactgacct atccggtatc
    27661 cgtcatcgcc gtcgccatcg gtttggtatt cgtgatgatg attttcgtac tgcccgcctt
    27721 taaagaagtt tacgccaata tgggcgcgga gcttcccgca ctgacccaaa cagtgatgga
    27781 tatgtccgac tttttcgtct catacggctg gatggtgctg atcgcactgg gctttgccat
    27841 atacggcttc cttaaattga aggcgcgttc gattaaaatc caacggcgta tggatgccat
    27901 actgctgcgt atgccgattt tcggagacat tgtccgcaaa gggacgattg cccgctgggg
    27961 caggacgacg gcgacgctga ttgcggcagg cgtgcctttg gtcgatgtat tggactccac
    28021 tgccggcgcg gcgggcaatt taatctatga agaagccacc cgggaaatcc gtacgcgcgt
    28081 catccaaggt ttgtctatga cttcggggat gcgtgcgacg gaactgttcc ccaatatgat
    28141 gttgcagatg tcttccatcg gcgaggaatc gggttctttg gacgatatgc tcaacaaagc
    28201 cgccgaattt tacgaagacg aggtggacaa tgcggtcggc aggctgtccg ctatgatgga
    28261 gccgatcatt atcgtgattt taggcttggt catcggcacg cttctggtcg ccatgtatct
    28321 gccgctgttt aatttgggca acgtggtcgc ctgatttgcc gcacagatcc ggcgcggatt
    28381 cgctctgcgc cggtttgttt ttgttttgaa tacattgaga acaaaatatg tctgatttgt
    28441 ctgtattgtc gccgtttgcc gtgcctttga cagcggtgtt cgggctgctg gtcggaagtt
    28501 tcttaaatgt cgtcatttac cgcgtgccgg tcatgatgga acgcggctgg acggtatttg
    28561 ccaaagaata tttaaacctg ccgctgaccg aagaggaaag ccgtaccttc aacctgatga
    28621 aaccggattc ctgctgtccc aaatgccgcg tgccgatacg cgcgtggcag aacatcccga
    28681 ttgtcagcta cctgctcctg cgcggcaaat gcgcctcctg ccaaaccaaa atcagcatac
    28741 gctatccctt aatcgaactg ctgaccggcg tattgttcgg gctggttgcc tggcaatacg
    28801 gctggtcttg gattacattg ggcggtttga tactgaccgc gtttctgatt tccctgacct
    28861 ttatcgatgc ggacacccaa tacctgcccg actcgatgac attacccttg atttggctgg
    28921 ggctgatatt taatttggac ggcggcttcg tgcctttgca gtctgccgtt ttaggtgcgg
    28981 ttgccggcta tggttcgata tggcttttat gcgcggtata taaactgatt aaaggcgaaa
    29041 tcggtatggg caacggagat ttcaaactga ttgccgcatt gggcgcgtgg ctcggcatat
    29101 ccgcattgcc cgtgctgatt tttgtttcct cgctgatcgg tttggtcgcg gcaatcgtta
    29161 tgcgcgtcgc caaggggcag cattttgcct tcggccccgc actgacagtt tcgggctgga
    29221 taatttttac ggcaaacgat tccgtatggc gggcggtcaa ctggtggctg acccatccgg
    29281 tgctgtaaga tgacggtatg ggtcggactg accggcggaa tcggcagcgg caaatcggca
    29341 gccgcgcaat gttttgccga tttgggcgta ccgcgtatcg atgcggatgc ggcggcgcac
    29401 tcgctgacgg cttcagacgg catcgccctg ccggaaatca ggcggctgtt cggcgacacc
    29461 gttttcgaca cacagggttt gttgcgacgc gacatattgc gtaaagaagt ctttgcctcc
    29521 ccatcgcgaa aagccttgct cgaatccgtg atgttgccgc tgattttctc agaaatcaaa
    29581 aaacagcaag aaacctttac cgatgcggtt tacggcattg tcgaaattcc gctgctgacg
    29641 gaaaagcgtc aatttatcag cctgatacgg cgtgtcctga ccataagtgc ccctgtggaa
    29701 aaacgtatcg gcagggtgat ggcccgcagc gggctgacgc gcggcgaggt ggcggccgtc
    29761 atcagccatc aggcatccga atccgaacgc ctgctgcttg cagacgatgt gctgctcaat
    29821 gacggcagcc tcaaaagcct gcgtgagaaa acaatgcgcc tgcacgcgtt ttattcaggg
    29881 attttcgcct caaaaccaac acaaggaaaa cacaatgact gaatcgcggc aaacacgcct
    29941 tcaagtcaaa tgtccgacct gtcaaacggc agtagtatgg aaaccggaaa acgcattccg
    30001 ccccttctgt tcgcaacgct gcaaactgat cgacttgggc ggatgggcag acgggaaata
    30061 tacggtttcc agccaaacgg aaagtttgcc ggaaatatcc gaacccgaca tggcataccg
    30121 ctgaccgccc cgccttcccg gcaaacaccc tgaaagtcaa atgccgtctg aaacaaacac
    30181 gcttcagacg gcattttcat tctcaaacct aatcgttggt atttgccgtt acctcttcca
    30241 atgaagtaat gccctgcata actttcaaaa taccggcccg gcgcaaatcc accataccct
    30301 ccttataggc aacgtccaaa atatccactt ccgtaccgtt gttcataatc acacgctgca
    30361 tttcttcgct gatgggcata acctcataca cgcccgcacg ccctttataa ccctgccccc
    30421 ggcaacggtc gcaaccgacg gcgcggtaaa gtttccaatc ttttgcaagg tcctcatcgg
    30481 tgaagccgac ttccttcaaa gcagaggcag acgggcgttc cacttcctgt ttgcagctcg
    30541 aacacagcct gcgtaaaaga cgctgcgcca taatcaggct gaccgaactg gcaatattaa
    30601 acggcgcgac acccatattc agcatacgcg acaacgtcgc cggcgcatta ttggtgtgca
    30661 gggtggaaaa caccatatgc cctgtttgtg ccgccttaat cgcaatatcg gcagtttcca
    30721 aatcacgaat ctcaccgacc ataatgatgt ccgggtcctg acgcaggaaa gacttcaaag
    30781 cagcggcaaa agtcaggccc tgcttatcat tgacgttaac ctgattgatg cccggcaggt
    30841 taatctcggc agggtcttcc gccgttgcaa tatttaccga ctccgtattc aaaatattca
    30901 aacaggtata gagcgacacc gtcttacccg aacccgtcgg accggttacc agcaccatcc
    30961 cgtagggacg gtgaatcgct tccaacaaca attttttctg aaacggctca aaaccgagct
    31021 ggtcgatgtt caaagacgcg gcatcggaat tcaaaatccg catcacgacc ttttcgccaa
    31081 acagcgtcgg caatgtgctg acacggaaat cgacaggctt gccgcccttt tgaaaggtca
    31141 gctgcatcct gccgtcctgc ggtatccgtt tttcggaaat gtccaaacgc gacattacct
    31201 taatccggga agcaagctgc ccccttaccg caatgggcgg ctgaaccacc tcgcggagct
    31261 gcccgtccac acggaaacgg atacgggcat tgtgttcgta aaactcgaaa tggatgtccg
    31321 atgccccgct gcgcaaggca tccgacaaag tcttatggat aaacctcgga acagggccgt
    31381 cttctgcctc ctcgttgtcg atatacaggg tgtggctttc ctcttcctcc tgcccctccc
    31441 caagctcctg aagcagcgat gtcgaacgcg aacccaccca atcgagcaaa cccgccaact
    31501 ggtcatcctc gacaatgacc aattcaacct caatccctgc ggcagaaacg gttttctgaa
    31561 tttgcggcat ctgtgtcgga tcggaaaccg caaaaaatac tttgtcgccc cgacggaaaa
    31621 ccggcacaca gtggaactcc accatctgct cctccgtcaa cacccccatc agcaccctgt
    31681 ggcgcggata atgacgcaaa tcaagaatcg aataactgaa caccctcgca atcaatgccg
    31741 caagcgactt gggcgaaatg acaccgtctg aaaacagcat cggcaacact tccttacccg
    31801 cctgcgactc attgtagtaa tgctcggcct gctcaacagt aaccacctgg ttttgaacca
    31861 gaatcctcag caaacctacg ctcatacgac cttatcccca aatttattca ttgttatacc
    31921 tgtacagctt
//
LOCUS       DAWFKN010000015        30863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFKN010000015 DAWFKN010000000
VERSION     DAWFKN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.015"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            80..1045
                     /locus_tag="WGR46_000699"
     CDS             80..1045
                     /locus_tag="WGR46_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0855684.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSIDKNRLPYLDEYFKSTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKKAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1209..2177
                     /locus_tag="WGR46_000700"
     CDS             1209..2177
                     /locus_tag="WGR46_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0855685.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADRLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2167..3141
                     /locus_tag="WGR46_000701"
     CDS             2167..3141
                     /locus_tag="WGR46_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0855686.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3186..3812
                     /locus_tag="WGR46_000702"
     CDS             3186..3812
                     /locus_tag="WGR46_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855687.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASADELKQFGELWQERVRAFLIDQADNPEFVTVSEI"
     gene            3893..4651
                     /locus_tag="WGR46_000703"
     CDS             3893..4651
                     /locus_tag="WGR46_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855688.1"
                     /translation="MITIRNVSHTIGSNPILNDVSLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4710..5048)
                     /locus_tag="WGR46_000704"
     CDS             complement(4710..5048)
                     /locus_tag="WGR46_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0855689.1"
                     /translation="MKKIEAVIKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5143..5355
                     /locus_tag="WGR46_000705"
     CDS             5143..5355
                     /locus_tag="WGR46_000705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855690.1"
                     /translation="MPETVIPAQAGIPTSAFQQYLKVSVNPNFHIPDRAETTAFDPKT
                     YGSSCPLLCFLQLSAYPALGKNQKNR"
     gene            5513..9475
                     /gene="purL"
                     /locus_tag="WGR46_000706"
     CDS             5513..9475
                     /gene="purL"
                     /locus_tag="WGR46_000706"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0855691.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGNAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFTDPNTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQITQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9570..10322
                     /gene="gloB"
                     /locus_tag="WGR46_000707"
     CDS             9570..10322
                     /gene="gloB"
                     /locus_tag="WGR46_000707"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0855692.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            10556..12010
                     /gene="mgtE"
                     /locus_tag="WGR46_000708"
     CDS             10556..12010
                     /gene="mgtE"
                     /locus_tag="WGR46_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0855693.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGR46_000709"
     CDS             complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGR46_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0855694.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13249..14004
                     /locus_tag="WGR46_000710"
     CDS             13249..14004
                     /locus_tag="WGR46_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0855695.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14023..14241
                     /locus_tag="WGR46_000711"
     CDS             14023..14241
                     /locus_tag="WGR46_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855696.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14386..14730
                     /locus_tag="WGR46_000712"
     CDS             14386..14730
                     /locus_tag="WGR46_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0855697.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            14730..15422
                     /locus_tag="WGR46_000713"
     CDS             14730..15422
                     /locus_tag="WGR46_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0855698.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            15489..16709
                     /gene="argJ"
                     /locus_tag="WGR46_000714"
     CDS             15489..16709
                     /gene="argJ"
                     /locus_tag="WGR46_000714"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0855699.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            16769..18178
                     /locus_tag="WGR46_000715"
     CDS             16769..18178
                     /locus_tag="WGR46_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0855700.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            18368..19759
                     /gene="hrpA"
                     /locus_tag="WGR46_000716"
     CDS             18368..19759
                     /gene="hrpA"
                     /locus_tag="WGR46_000716"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0855701.1"
                     /translation="MSPNFAQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            19860..20987
                     /locus_tag="WGR46_000717"
     CDS             19860..20987
                     /locus_tag="WGR46_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855702.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQCPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQGTQVLRIDGTKIVYIK"
     gene            21164..22741
                     /gene="lpt3"
                     /locus_tag="WGR46_000718"
     CDS             21164..22741
                     /gene="lpt3"
                     /locus_tag="WGR46_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0855703.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPNKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            22807..26106
                     /locus_tag="WGR46_000719"
     CDS             22807..26106
                     /locus_tag="WGR46_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ0855704.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VPYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSAAASAVSNDLHPANPQQTAPSPVGEGREEGKTVASQTNFSATTA
                     NPLPNPLPQEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERE
                     NPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLT
                     VLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVI
                     IPQLAHFIAKSAGDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHEL
                     QQQLGQAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGR
                     IALRLCDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLR
                     DDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELN
                     GKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDA
                     AREADIQELEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSV
                     KRLLKEWEDLN"
     gene            complement(26129..26551)
                     /locus_tag="WGR46_000720"
     CDS             complement(26129..26551)
                     /locus_tag="WGR46_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0855705.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(26555..28201)
                     /locus_tag="WGR46_000721"
     CDS             complement(26555..28201)
                     /locus_tag="WGR46_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0855706.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28215..29270)
                     /locus_tag="WGR46_000722"
     CDS             complement(28215..29270)
                     /locus_tag="WGR46_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0855707.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            29560..29772
                     /locus_tag="WGR46_000723"
     CDS             29560..29772
                     /locus_tag="WGR46_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855708.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            30082..30237
                     /locus_tag="WGR46_000724"
     CDS             30082..30237
                     /locus_tag="WGR46_000724"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0855709.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(30327..30776)
                     /locus_tag="WGR46_000725"
     CDS             complement(30327..30776)
                     /locus_tag="WGR46_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0855710.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7558 a         9001 c         7320 g         6984 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caaaccgaag gcgcgtccct gaccgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccat cgataaaaac cgcctgccct acttggacga
      361 atatttcaaa tccacgaaac ctgccggcac gctgtttgag ccggattacg aaacgctcaa
      421 cgcttacaaa ccgcagctca tcatcatcgg cagccgcgca gccaaagcgt ttgacaaatt
      481 gaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa aaggcggaag ccgacaagct
      601 gaaggcggaa atcgacgcgt cttttgaagc cgcgaaaact gccgcgcaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa aatgtccgcc ttcggtccgt cttcacgctt
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgaa
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtcgtgt acctcgttcc
      961 tgaaacttat ttggcagccg gtggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 cgacgctttt aacgcggcaa aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cctcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac caacctgctg ctgctggcgg
     1261 tgttgtttgc cgtcagcctg tcggtgggcg ttgccgattt ccgctggtct gatgtgtttt
     1321 cactgtccga cagccagcag gtcatgttca tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattttg atgcgtaacc
     1441 gttttgtcga gccttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgttaatcgg gatgttggtg tttatgatgc ttatccgccg cctgccgccg acggcgcaac
     1621 tgatggtgcc tttggtcggg attattttcg gcggcgtggt tgaggcggtg gccaccttta
     1681 ttgcgtatga aaacgaaatg ctgcaaatgc tgggcgtgtg gcaacagggc gatttttccg
     1741 gcgtgttgct cggacggtat gaactgttgt gggcaacggg gattttggct ttgtttgcct
     1801 atttgattgc cgaccggctg acgattttgg gtttgggcga aacggtaagc gtgaacttgg
     1861 ggctgaaccg gacggcgatt ctgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1921 tggtcatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     2041 ctttggtgtt gctgtgcgac attatcggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tctctacggt ttttggtgtg ttgggtacgg ctttgttttt gtggcttttg ttaaggaaac
     2161 ctgctcatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     2221 gttgcctttg cgctgttgct ggtttcctgc atcctgttta tgacgctcaa cgtcaaaggc
     2281 gattgggact ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtttcgac ccagcttttt caaacgctga ccaacaatcc gattctcacg
     2401 ccctcgattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtc
     2521 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2641 ctgctttcgc gcatgatcga tcccgaagaa tttaccgccg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2761 gcggcagcgg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     3001 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     3181 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     3241 aagacccgct tgccgcattc ctcggcgcag cgcaagacgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3361 ttatcaaagg tctgaaagcc ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggaacgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3481 tcctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatgcag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3601 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgcgctccaa ccctttgcac
     3661 cgcaaatgcg cgacatcatg cctaaagccg tcagcggcag cgcaagtgcc gacgaactca
     3721 aacaattcgg cgagctttgg caagaacgcg tccgcgcctt cctgattgac caagccgaca
     3781 atcccgaatt tgtcaccgtc agcgaaatct aaaacgtcat aatcaataag tttcagacgg
     3841 ccctaaaact caaggccgtc tgaaacccca atttccattt tcggacccgc ctatgattac
     3901 catccgcaac gtcagccaca ccatcggcag caaccccatc ctcaacgacg tcagcctcga
     3961 cattcccgaa ggcggcatta ccgcgctgat cggcccgaac ggcgcaggca agtccaccct
     4021 cctctccttt atggcgcgcc tacagcctct ggtacacggc aacatcagct acgcaggcaa
     4081 agacatcaaa accaccccta ccgccgaact cgcccgcacg ctctccatcc tcacccaaga
     4141 aaacagcatc atgagccgca ttaccgtgcg cgacctgctg atgttcggcc gttaccccta
     4201 ccatcaaggc agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt
     4261 ccacctgcaa gacctctccg accgctatct gaccgagctc tccggcggcc aacgccaacg
     4321 cgccatgatt gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct
     4381 gaacaacctc gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgcg
     4441 cgaacacaaa cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc
     4501 cgaccacgtc gtcgccatga aaaacggcaa agccgtcctg accggcacgc ccgaagaagt
     4561 gttcaccgta cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga
     4621 aggcaaaaaa ctcatcgtcc accatatctg aagctcaaaa agaaaatgcc gtctgaaagg
     4681 ctttcagacg gcatttccat tccaaacgct caaaccgccg cgtccgcacg ttcgcccgtg
     4741 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gatttttccc
     4801 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcatcatc cgccaacaca
     4861 agctcgattt tgaccttggg caggaaatcg acggcgtatt ccgcaccgcg atagatttcc
     4921 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatgcc cgtaatgccg
     4981 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgataac agcttcgatt
     5041 tttttcatca cattctccat taattaacgt aaacaaacga caaatatagc agaaaccgcc
     5101 ggcaaacgat aaacggtact gttcgcccgg cgatgcggcg atttgccgga aaccgtcatt
     5161 cccgcgcagg cgggaatccc gacctctgca tttcagcaat atttaaaggt ttctgtaaat
     5221 ccaaacttcc atattcccga ccgtgcggaa acgacggcat ttgatccaaa aacatacgga
     5281 agcagttgcc cgcttctctg cttcctgcaa ttatccgcat acccggcttt gggtaaaaat
     5341 cagaaaaaca gataaaaccc ttattcctta atatcttcag acggcacctg tattttcccc
     5401 aaccgtatgc cgtctgaaga ttgtcaacat tttaagccaa aatatccaag caatacaaac
     5461 ctgtccggcg tataatccct ctttttccca accgcatttg agagccgaat ccatgtctgt
     5521 cgttttgccc ttgcgcggcg ttaccgccct ttccgatttc cgtgtcgaaa aactcttgca
     5581 aaaagccgcc gcactcggtc tgcccgaagt caaattaagc agcgaatttt ggtatttcgt
     5641 cggcagcgag aaagcacttg atgccgcgac tgtcgaaaaa ctgcaagcct tgttggcggc
     5701 gcaaagcgtt gaacaaacgc caaaagcgcg cgagggcttg catttgtttt tggtcacgcc
     5761 ccgtttgggt acgatttcgc cgtgggcttc caaggcgacc aatatcgcgg aaaactgcgg
     5821 tttggcaggc attgagcgta tcgaacgcgg catggcggtg tggctggaag gtgcgcttac
     5881 cgacaggcaa aaacaacaat gggcagccct gttgcacgac cgcatgaccg aaagcgtgtt
     5941 gaccgacatc gatgcggcgg cgcaactgtt ccaccacatc caatcggaaa ctttctccac
     6001 cgttgatgtt ttgggcggcg gtaaagaagc tttggtcaaa gccaataccg aaatgggctt
     6061 ggcactctcc gccgacgaaa tcgattatct ggttgaaaac tatcaggctt tgcagcgcaa
     6121 tccgtccgat gttgaattga tgatgttcgc gcaggcaaac agcgaacact gccgccacaa
     6181 aatcttcaac gccgatttca tcctcaacgg cgaaaaacaa ccgaaatccc tcttcggtat
     6241 gatacgcgac acgcacaacg cgcatcccga aggcacggtc gtcgcttata aagacaactc
     6301 gtccgtaatc gaaggcgcga aaatcgagcg tttctatccg aatgcggcgg aaaaccaagg
     6361 ttaccgtttc cacgaagaag acacgcatat catcatgaaa gtggaaacgc acaaccaccc
     6421 gaccgccatc gcgccgtttg caggcgcggc aacaggcgcg ggcggcgaaa tccgcgacga
     6481 aggcgcgacg ggcaaaggtt cgcgtccgaa agcaggcttg accggcttta ccgtctccaa
     6541 cctcaacatc cccgacctca aacagccgtg ggaacaagac tacggcaagc cggaacacat
     6601 ttcctcgccg ttggacatca tgattgaagg ccccatcggc ggcgcggcgt tcaacaacga
     6661 attcggccgc cccaacctct tgggctactt ccgcactttt gaagagaagt ttgacggtca
     6721 ggttcgcggc taccacaaac cgattatgat tgccggcggc ttgggcagca ttcaggcgca
     6781 gcaaacacac aaagacgaaa tccccgaagg cgcgttgctg atccaactgg gcggcccggg
     6841 tatgcttatc ggcttgggcg gcggcgcggc ttcttcgatg gataccggta caaacgacgc
     6901 ttctttagac ttcaactccg tacaacgcgg caaccccgaa atcgaacgcc gcgcgcaaga
     6961 agtcatcgac cgctgctggc agcttggcga caaaaacccg attatttcca tccacgacgt
     7021 cggcgctggc ggcttgtcca acgccttccc cgaactggtc aacgatgccg gacgcggcgc
     7081 ggtattcaag ctgcgcgaag tgccgttgga agaacacggt ttaacgcctt tgcaaatctg
     7141 gtgcaacgaa tcgcaagagc gttatgtctt gtcgattttg gaaaaagatt tggatgcctt
     7201 ccgcgccatc tgcgaacgcg aacgctgccc gtttgccgtg gtcggcacgg cgaccgacga
     7261 cggtcatttg aaagtgcgcg acgatttgtt ctccaacaat cccgtcgatt tgccgctgaa
     7321 cgtcttgctc ggcaaaccgc ccaaaaccac gcgtaccgac aaaacggtta cgccgtccaa
     7381 aaaaccgttt cacgccgacg atatcgacat caccgaagcc gcctaccgcg ttttgcgcct
     7441 gcctgccgta gccgccaaaa acttcctgat taccatcggc gaccgcagcg tcggcggcat
     7501 gacccaccgc gaccaaatgg tcggcaaata ccaaaccccc gtagccgact gcgccgttac
     7561 catgatgggc ttcaatacct atcgcggcga agcaatgtct atgggcgaaa aaccgaccgt
     7621 tgccctgttt gacgcgcccg cctcgggcag aatgtgcgtc ggcgaagcca tcaccaacat
     7681 cgcggcggtc aacatcggcg acatcggcaa tatcaaactc tccgccaact ggatggcggc
     7741 gtgcggcaac gaaggcgaag acgaaaaact ctaccgcacc gtcgaagcgg tttccaaagc
     7801 ctgtcaggca ttggatttga gcattcccgt gggcaaagac agcctgtcga tgaaaaccgt
     7861 atggcaggat ggcgaagaga aaaaatccgt cgtttcgccg ttaagcctga ttatctccgc
     7921 gttcgcgccg gttcaagacg tacgcaaaac cgttacgccc gaattgaaaa acgtcgagga
     7981 cagcgtattg ctgtttgtcg atttgggctt cggcaaagcg cgtatgggcg gctctgcatt
     8041 cggtcaggta tacaacaaca tgagcggcaa cgcgcccgat ttggacgata caggccgtct
     8101 gaaagccttt tacaacgtga ttcaacagct tgtcgccgaa gacaaactct tggcgtatca
     8161 cgaccgcagc gacggcggtt tgtttgccgt tttggtagaa atggcgtttg cagggcggtg
     8221 cggcttggat atagatttaa atttattgct tgcacaaaca tttattacca accataccgc
     8281 tctgtctcaa tcattgcgga ctgaagaggt aaaagcgttg gctgaatggc aagaaaccat
     8341 tgcccgcaca ttatttaatg aagagttggg tgctgttatc caagttagaa aacaagatgt
     8401 tgccgatatt atcaatttat tctatcaaca acagctgcat cataatgtct ttgaaatcgg
     8461 tacgttaact gatgagaaca cgttaatcat ccgcgacggg caaacgcacc ttatttctga
     8521 caacctaatc aaactgcaac aaacctggca agaaaccagc catcaaatcc aacgcctgcg
     8581 cgacaaccct gcctgcgccg acagcgagtt tgccctgatt ggcgacaacg gacgcagcgc
     8641 attgtttgcc aacctgaaat tcgacgtgaa cgaagacatc gccgcgccgt ttatcaacag
     8701 cggcgcgaaa cccaaaatcg ccatcctgcg cgaacagggc gtaaacgggc aaatcgaaat
     8761 ggccgccgcc ttcacccgtg ccggtttcga tgcctacgac gtgcatatgt ccgacctgat
     8821 ggcaggccgc gtccaccttg ccgacttcaa aatgctggcg gcgtgcggcg gcttcagcta
     8881 cggcgacgta ctcggcgcgg gcgaaggctg ggcgaaatcc atcctgttcc accccgcctt
     8941 acgcgaccag ttcgccgcct tcttcaccga cccgaacacg ctgacattgg gcgtgtgcaa
     9001 cggctgccag atggtcagca acctcgccga aatcatcccc ggcacggcag gctggccgaa
     9061 gttcaagcgc aacctgagcg aacagttcga agcgcgcctg agcatggttc acgtccccaa
     9121 atcagcctcc ctgattctga acgaaatgca aggctccagc ctgcccgtcg tcgtcagcca
     9181 cggcgaaggc cgcgccgact tcgcgcttca cggcggcaac atttctgccg atttgggcat
     9241 tgcgttgcaa tatgtggacg gacaaaacca aattacccaa acctacccgc tcaaccccaa
     9301 cggctcgccg caaggcatcg ccggcgtgac caacgccgac ggccgcgtta ccatcatgat
     9361 gccgcatcct gaacgtgtat accgcgccgc acaaatgagc tggaaaccgg aagactggac
     9421 ggaattgtcc ggctggtacc gcctcttcgc cggcgcacgt aaagccttgg gctaaccgcc
     9481 ctactcaaac caatgccgcc tgaagaatat ttcagacggc gttccggcat accatccttt
     9541 aaacggtatc cgtccaccga ggaacactca tgaaaatcac acccgtcaaa gccttgaccg
     9601 acaactacat ctggatgata cagcacggca accatgccgt ctgcgtcgac ccttccgaac
     9661 cctcgcccgt cttggaattc ctcgtccgca accgcctcat gcttgcccaa acatgggtaa
     9721 cgcaccccca ccccgaccac gagggcggtg cggcggcact ctggcgcggc tacatggaat
     9781 cgcccgttta cggcgaatcc gacatcgaag cagcaaccca caccgtaacc gccggcaccc
     9841 aattcacctt cggcgacgga caggttaccg tttgggcaac acccggccac acagaccgcc
     9901 acaccagcta ccttctcgaa acttcagacg gcatacacgt cttttgcggc gacacccttt
     9961 tttccgccgg ctgcggacgc gtgtttaccg gcacaatcga acagctttac gacagcttcc
    10021 aacgcttcaa ccgcctgcct gaaaacaccc tgttctaccc ggcacacgaa tacaccgccg
    10081 ccaacctgcg tttcgccgcc catatcgagc cggacaacgc cgacattcag acggcattga
    10141 aagcggcaga acgcacgccc accctgcccg ttaccctcgc ccacgaacgc cgcgtcaacc
    10201 cgttcttacg gacagaaatc cccgccgtcc gccaacgtgc cgaagccctg gtcggcaaaa
    10261 cgctgaacag cggtttggaa gtattcgccg ccctgcgcga actgaaaaac gcctactgct
    10321 gacctgccct ccgaaaaatg ccgtctgaaa cccgcgtttc agacggcatt tgcgttaaaa
    10381 atagtaaacc gtttcaaaag ggagtagaat agtgccgttt ccaaccctgc gcctgtaccg
    10441 tcaggctttt attatggacc ttcccagttc gtttttactg aacacccctt ccgattccaa
    10501 acgacaataa ccatcccgcc ggaatgcctc cccgcgcacg gcgggcggag catttatgag
    10561 catcgaacca acccctccga accttgaaaa cgacggtatc gaaaacgatg tagaacgcgt
    10621 ttccgccgat ttcgaccgtg tccactccct ctgcgaaatc ctcgaacctg cttttgaaca
    10681 aatcgaaaac ggcacaccgc tcgaagacgc gccgctgcgc gacaagctga ccgagctgac
    10741 cgtcctcttg ggcgagctgc accctgccga cgtggcggcg gtgttggaat cgctgccgcc
    10801 gcgcgaacgc aacgtcgttt ggcttctggt caaacccgaa gacgacggcg aagtattgct
    10861 ggaagtatcc gacgcggtgc gcgaaacact gatcgagtcg atggataagg acgaattgtt
    10921 ggcggcagtt gacgatttgg acgcggacga attggcggaa ctggcagacg atttgccgca
    10981 ccaagtggtt tacgaagcct tacagacgcg tgatgaggaa gaacgcgccc aagtcaaggc
    11041 ggcaatgtcc tacgaagaca accaagtcgg tgcgattatg gacttcgagt tggtcagcat
    11101 ccgcgccgat gtcgcctgcg aagtggtgct gcgttacctg cgccgcttcg acagcctgcc
    11161 cgaccacacg gataaaatct tcgtggttga cgaaaacgat gtgttgcagg gcgtgctgcc
    11221 catccgcaaa cttttggtcg ccgatcccga agacttggtg gaaaacgtga tggcgaaaga
    11281 tgtcgtgcgt ttccgcgccg aagacgacgt ggaagaagca gcgcaggcgt ttgaacgcta
    11341 tgacttggtt accgcgcccg tcgtcgatga aaacaaaaag ctcatcggca gggtgaccgt
    11401 tgacgaaatg gtggacgtga tccgcgaaga atccgaagca gatatgttga acatggcggg
    11461 tttgcaagaa gaggaagact tattcgcccc cgtcttggat tccgtcaaaa accgctggat
    11521 gtggctcgcc gtcaacctct gcaccgcctt ccttgccagc cgcgtcatcg gcgcgtttga
    11581 aggcagcatc gaaaaaatcg tcgcactcgc cgcgctgatg cccatcgtcg ccggtatcgg
    11641 cggcaactcg ggcaaccaga cgattaccat gattgtccgc gccatggcga tggggcaact
    11701 gacggatatg caggcggggc gtttgctgaa aaaagaagtc ggcgtcgcct tggtcaacgg
    11761 catcatttgg ggaacggtca tgggcgcagt atcttggctg ctttacggca gcctcggcat
    11821 cggtttggtc atggttgccg cgatgacgct caacctgctg ctggcggcaa ccgtcggcgt
    11881 attaattccc gtggtaatgg aaaagttcgg acgcgatccc gcgctgggca gctcggtgct
    11941 gattacggcg gtaacggatt ccggcggctt cctgattttc ttgggtctcg ccaccctgtt
    12001 cctgctttaa atgccgtctg aacccgcgca aaaatgccgt ctgaagcgga agctgcttca
    12061 gacggcattt gactatttat ccttgttgca caagattatt ggacggtatg ccggggcagc
    12121 cctttggcaa cgccgaccac atcctccccg aacagcgcgt tgacatcggt ttcgtcaaac
    12181 acatatttgc tgtggcagaa atcgcaatcg acttcgatgc tgccttgttc caccaccacg
    12241 ccgccgactt cttccccgcc cagcatcagc agcatatcgc tgactttgcc gcgcgaacag
    12301 gtgcatgaaa attcaaacgt ttccggctcg aacacgcgcg gcggcgtttc gtggaacagg
    12361 cggtacaaaa catgttgcgc atccaattct gccagctcgt ccgccgtcag tgtacgcgcc
    12421 agcgtactga cgtgttccca tgcctcttca tccaacaccg cttcaggcag acgctgcacc
    12481 agcagaccgc ccgccgcttc gtcgcttgca gacaggacga tgtgcgtatc gatttgttcg
    12541 gaacgcttca tatagttcgc caacatttgc gcgataccgc cgccttccaa aggcactacg
    12601 ccctgccagg gttcgccgtc tttgggctgc agcgtcagca cgaatacgcc gccctcgccc
    12661 aaaaggtcgc cgaggctttc gtcatcggct atttctgcgg tttcgtccca acgcgcggtt
    12721 gcacggacgg tacggtcgga agccgcttcc gcaaccagca ttttcagccg cccccgcccc
    12781 tgaacctgca caatcagcgt gccttcgttt ttgaggttgc ccgacagcaa cacacccgcc
    12841 gccaacaact cacccaaagc gcggcggatg gcggcgggat agtttttctg ttttacaatg
    12901 tgctgccaca cgttttccag acggacgtgc agcccgcgca cgggcatatc gtcgaagata
    12961 aagcgggtac gcacatcggc gcggttgatg gcggtttgat tcatgatttt ctctgactga
    13021 ttgttcggat ggcggctata tggttgcggt cggcgcgaaa acaagacgga cggcggatgc
    13081 gcttcccaaa ttatcaataa attatataaa aatcaacata ttaactcaat ctaacaagcc
    13141 gttttttgcc aaacagccgt ttttttatat acaatcaaca agatattttc gactgataca
    13201 gcataacatc gcacggcggc acgatgcctc ctgcgcggaa acaccgatat ggattctttt
    13261 ttcaaaccgg cagtttgggc ggttttgtgg ctgatgtttg ccgtccgccc cgcccttgcc
    13321 gacgagttga ccaacctgct cagcagccgc gagcagattc tcagacagtt tgccgaagac
    13381 gaacagcccg ttttacccat caaccgagcc cccgcccggc gggcgggcaa tgccgacgaa
    13441 ctcatcggca gcgcgatggg gcttaacgaa cagcccgttt tacccgtcaa ccgagtcccc
    13501 gcccggcggg cgggcaatgc cgacgaactc atcggcaacg cgatggggct taacgaacag
    13561 cccgttttac ccgtcaaccg agcccccgcc cggcgggcgg gcaatgccga cgaactcatc
    13621 ggcaacgcga tgggactttt gggtattgcc taccgctacg gcggcacatc gatttctacc
    13681 ggttttgact gcagcggctt catgcagcac atcttcaaac gcgccatggg catcaacctg
    13741 ccgcgcacgt cggcagaaca ggcgcggatg ggtacgccgg ttgcccgaag cgaattgcag
    13801 cccggggata tggtgttttt ccgcacgctc ggcggcagcc gcatttccca tgtcggactt
    13861 tatatcggca acaaccgctt catccacgcg ccgcgcacgg ggaaaaatat cgaaatcacc
    13921 agcctgagcc acaaatattg gagcggcaaa tacgcgttcg cccgccgggt caagaaaaac
    13981 gacccgtccc gctttctgaa ctgatttccc aaggaatacg caatgagtat gcccgaaatg
    14041 cccaaatggt acgacgatga cggacagatc gtgtcctgta ccgaaaaggt caaagtgatg
    14101 tccgaaaata tggccgagct gtatcagacg gcacaagacg cgtttgaaga cgcgctgctg
    14161 atgggttgcg gcgaacgtca gttgcgcgat tacctgctcg cgctgattga aggtttggaa
    14221 aatccctacc gcaaagtctg aacacgcccc ggttgctgcg gcacggttta tccgtgccgt
    14281 ttttgcgttt gtgcgcggct tcggcttttc agacggcata tttgacgtta tgattaaaca
    14341 gttaacaaga tttatcacaa cgccgtcaaa agacagacac acaacatgaa catcatccgc
    14401 gcgctcctca tcatcctcgg ctgcctcgcc gccggcgaaa ccgccgtttt cctagcaggc
    14461 atcaaactgc ccggcagcat cgtcggcatg ggcgtgctgt ttgcgctttt gcaggcaggt
    14521 tgggtcaaaa cgtcttggct acaacagctt accgacgcgc tgatggcgaa tctgacgctg
    14581 tttctcgtgc cgccctgcgt ggcggtcatc agctatttgg atttgattgc cgacgattgg
    14641 ttttcgatac tggtttccgc ctccgccagc actttgtgcg tactgctggt tacaggcaag
    14701 gttcaccgct ggatacggag cattatctga tgaacgaaat cctcaggcag cccagcgttc
    14761 tgcttttcct cacgcttgcc gtgtacgcgc ttgcgattat cgtgcgcacg cgcacgggca
    14821 atatcttctg caaccccgta ctcgtcagca ctatcgtgct gattgcctac ctgaaaatcc
    14881 tcgacatcga ttatgcggtg taccacaacg ccgcgcaatt cattgatttt tggctgaaac
    14941 ccgccgtcgt cgtgcttgcc gtgccgctct accaaaaccg ccgtaaaatc ttcaaccagt
    15001 ggctgcccgt catcgtttca cagcttgcgg gcagcgttac gggcattgtt acggggatgt
    15061 attttgccaa atggctgggc gcggaacgcg aagtcgtcct ctcgctcgcg tccaaatctg
    15121 ttaccaaccc catcgccatt gaaatcaccc gctccatcgg cggcattccc gccattaccg
    15181 ccgccaccgt catcattgcc ggtctggtcg gacagattgc cggttacaaa atgctgaaga
    15241 acacagtcgt catgccctcg tccgtgggta tgtcgctcgg cacggcttcg cacgcgatgg
    15301 ggattgccgc ctcgctcgaa cgcagccgcc gcatggcggc atacgcggga ctggggctga
    15361 cgttcaacgg cgtactgacc gcgctgattg cgccgctgct catccccgtt ttgggatttt
    15421 gaacccgttt cagacggcat ttcagcccat gctgtctgaa cgccgacaca ctcgcaagga
    15481 gaaccgttat ggctgtcaac ctgaccgaaa aaaccgccga acaactgccc gacatcgacg
    15541 gcattgccct ctacaccgcc caagcaggcg tgaagaagcc cgggcatacc gacctgacac
    15601 tgattgccgt agccgccggc agcaccgtcg gtgcagtctt cacgaccaac cgtttctgtg
    15661 ccgcgcccgt ccacatcgcc aaatcgcacc ttttcgacga agacggcgtg cgcgccctcg
    15721 tcatcaacac gggcaacgcc aacgcgggta cgggcgcaca gggcagaatc gatgctttgg
    15781 cagtgtgtgc cgccgccgcc cggcaaatcg gctgcaaacc gaaccaggtg ctgcccttct
    15841 ccaccggcgt gattctcgaa ccgctgcccg cagacaaaat catcgccgcc ctgcccaaaa
    15901 tgcagcctgc cttctggaac gaagcggcac gcgccatcat gaccaccgac accgtgccca
    15961 aagccgcctc gcgcgaaggc aaggtcggcg acaaacacac cgtccgcgcc acgggcatcg
    16021 ccaaaggctc gggcatgatt catcccaata tggcgaccat gctcggtttc atcgccaccg
    16081 atgccaaagt ttcccaaccc gtcctccaac tgatgacgca ggaaatcgcc gacgaaacct
    16141 tcaacaccat caccgttgac ggcgacacca gcaccaacga cagcttcgtc atcatcgcca
    16201 ccggcaaaaa cagccaaagc gaaatcgaca acatcgccga cccgcgttac gcccaactca
    16261 aagaattgtt gtgcagcctc gcgctcgaac tcgcccaagc catcgtccgc gacggcgaag
    16321 gtgcgaccaa gttcatcacc gtccgcgtcg aaaacgccaa aacccgcgac gaagcccgcc
    16381 aagccgccta cgccgtggca cgttcgccgc tggtcaaaac cgcctttttc gcctccgacc
    16441 ccaacctcgg caggctgctc gccgccatcg gttatgccgg cgttgccgac ctcgataccg
    16501 acctcgtgga aatgtatctc gacgatattt tggttgccga acacggcgga cgcgccgcaa
    16561 gctacaccga agcacaaggg caggcggtaa tgtcgaaggc cgaaatcacc gtccgcatca
    16621 agctgcatcg cggacaagcc gccgccaccg tctatacctg cgacctgtcg cacggatacg
    16681 tttccatcaa cgccgactac cgttcctgac ccgacacggc ttcagacggc atacataaaa
    16741 ttccgtctga accgccggac aacataccat gacctccaca ttcccccgcc gcctcgcccg
    16801 caaaatccgc caaacccgcc gcctgtcgcg caaaagcatc gcctttctgt tccttttggc
    16861 aggttcggca ctcgtcgccc tgaccgcgct gttttttgcc catcttgccg attttgcgct
    16921 ggaactgaac gccaaactgg ttcaacaata cccatggttc gcgtgggtcg cgcttccttt
    16981 gggtttgccg cttattgcgt ggctcacacg caaattcgcc cccttcaccg ccggcagcgg
    17041 catcccgcag gtcatcgcct cactgtcgct gccctacggc gcacagaaaa cgcggctgat
    17101 ccgcctcggg cagacactgc tgaagattcc gctgaccttt ttgggtatgc tgttcggcgc
    17161 gtccatcgga cgcgaaggtc cgtccgtgca ggtcggcgcg gcagtgatgg gcgcgtgggg
    17221 cgcgtggtgc aagaaacacg gcttggcatt caaagggatg caggaaaacg atttgatggc
    17281 ggcgggcgcg gcgggcggtt tggcagccgc gttcaacgcg ccgctggcgg gcgtgatttt
    17341 cgccattgag gaactcgggc gcggcatcat gttgcgctgg gagaggcaaa ttcttttggg
    17401 cgtgctcgcc tccggtttca tacaggtcgc cattcagggc aacaacccgt atttttccgg
    17461 cttcaacggc ggcgtattgg aacatatctt tctgtgggtc gcactgtccg gcctggtttg
    17521 cggcgcggcg ggcgggctgt tcggacgttt gctctatcgc ggtgcggcgg cgtttgcacc
    17581 gcgcaagata cgcggcttca tccgcaaccg tccgctgctg ctggcggcac tgatggggct
    17641 gctgctcgcc ctgctcggca cgttctacca aggcaaaacc tacggcaccg gctaccacga
    17701 agccgcccaa gccctgcacg gcatctacga agcccccttc ggactcgccg ccgccaaatg
    17761 gctcgccacc gtattcagct attgggcagg cgttccgggc ggcattttca ctccctcgct
    17821 gaccataggc gcggttttgg gcgagcatat cgccgccatc gccgacatat cgcagggtgc
    17881 aaacatcatc gtcctcatct gcatggcggc atttctggcg ggcgcgacac aatccccgat
    17941 tacttccgcc gtcgtcgtca tggaaatgac gggcggacaa agcctgctgt tttggatgct
    18001 gattgcctgc attttcgcct cgcaggtttc gcgccagttt tcgccgcgtc cgttctacca
    18061 cgcatcggga atgcgcttcc gccagcgcgt gcttcaagaa accgccgccc aaaccggcaa
    18121 tgcgcccgca agaccgcaaa cagcaaacag caaaacggga atgccgtctg aaaattaaaa
    18181 cgcccccgat caaacgccgg cagccgcctt gatttgaata ccgttccgcc gccgcttgaa
    18241 atttcagcaa caatgccgtc tgaacgacag aatgcggttt tcagacggca tttccccatc
    18301 ccgatattgc ccgaacccaa ccaaagcgtt tgctataatt tcatttttac cgtacccgca
    18361 cacaaatatg tcccccaatt tcgcccaaac cctctccaaa gaccgccact tcctgcaatc
    18421 cgccttcaaa aatcccaaca aatacggcgg cttgtccaaa gtcgaagaaa aataccgaaa
    18481 atcgcacgaa atctttttga aacgtttggc agccttgccc aaacccgaat tcgacaacac
    18541 cctgcccgtt cacgaaaaac ttgacgaaat caaaaaaacc gttgccgaac atcaggtaac
    18601 gattatttgc ggcgaaaccg gttcgggcaa aaccacgcag ttgcccaaga tttgtttgga
    18661 actcgggcgc ggggcggcag gattgatcgg gcatacccag ccgcgccgtt tggcggcgcg
    18721 ttcggtggca gagcggattg ccgaagagct gaaatccgaa atcggcagcg cggtcggcta
    18781 taaagtacgc ttcaccgacc acacctcgcg cgatgcctgc gtcaagctga tgaccgacgg
    18841 catcctgctg gcggaaacgc agaccgaccg ttatctcgcc gcctacgaca cgattatcat
    18901 cgacgaagcg cacgagcgca gcctgaacat cgacttcctt ttgggctatt tgaaacaact
    18961 cctgccgcgc cgccccgatt tgaaagtcat catcacctcg gcaacgatag acgcagaacg
    19021 cttctcccga cacttcaacg gcgcgcccgt tttagaagtg agcggacgga cgtatcccgt
    19081 cgaaatcctc taccgaccgc tgaccggcaa agacgaagac gacgcagaag tggagttgac
    19141 cgacgcgatt gtcgatgcgg cggacgaatt agcgcgacac ggcgaaggcg atattttggt
    19201 attcctgccg ggcgagcgcg aaatccgcga agccgccgaa gccctgcgca aatccacgct
    19261 gcgccgcaac gacgaaatcc tgcccctgtt cgcacgcctg tcgcacgccg agcagcacaa
    19321 aatcttccac ccctcaggcg cgaaacgccg catcgtattg gcaaccaacg tcgccgaaac
    19381 ctcgcttacc gtgccgggca tcaaatacgt catcgacacc ggcctcgcgc gtgttaaacg
    19441 ctattccgca cgggcgaaag tggagcagct tcatatcgaa aaaatctccc aagccgccgc
    19501 ccgccaacga tccggccgct gcggccgcgt ctccgcaggc gtatgtatcc gattgttttc
    19561 agaagaaaat ttcaacaacc gccccgaatt taccgacccc gaaatcgccc gcagcaacct
    19621 cgccgccgtc atcctgcgca tggcatcgct gaaactcggc gacgtagcgg cattcccatt
    19681 tttagaaatg cccgattcga ggtatattaa tgatgggttt caggtgttgc tggaattagg
    19741 ggcagtggag gccgcctgaa aacaagtagg atatacactt ctacctgaca aagaccaaca
    19801 aacgataaaa ctacggcaca tctagatgcc caacctacaa cattgcacac ggtaaaaaca
    19861 tgaaacactt actcatcgat tttgaaaacg tccagccgca aaacttagac aaattactaa
    19921 ccgaaaatac ccatatttgg ctatttatag gtgtattaca caaaatgtta cctattagtc
    19981 tggtgcaatc cctactacgt ttcggcgaac gtgtccatct tgtccagtta caaaaaacag
    20041 ggaaaaacgc attggatttt tacctgtcct attacctcgg acaaattacc gccacagacc
    20101 ccaatgccca aatcggcata ctctcgcgtg atggaggata cgatgttctg gtcgaacata
    20161 ttttgaaaaa ccaccaggcg aagggtatcg tgcgcctagc caatatagat gaagtacaac
    20221 atcagaaaat tactaccgaa ccgccgtcag cattgctgga aaacactcct cagcctgaaa
    20281 ccaccctcaa accacagcaa ccattaactt cctatttcca agcagcccta actgcactgc
    20341 gccgccccga cgctttccgc ccctgccgcc tgcataacct gcgacaaaat ctgcgtaagc
    20401 atattttgag tgatttgttt aaagaaaaaa ccgatgaaga atgcgaaata accactgcta
    20461 acgttatcaa taaactcaaa gcacaaaact tcatcagcat tgatgaacag gaaaccgttt
    20521 cctaccatct cagtgataat gatttgttac aaagaatcca acgccatatt ttaagccaat
    20581 gtcccaaaac ctacgctgat tttcaagccg tcgtgcaaaa ccgagcagat gcacttcact
    20641 taacagtcgg taccaacgac attcaatcct ttgcgcgaca tttgcgcgac caaaacctga
    20701 tccgccaaaa caatgggaaa attgaatatg caccgtttac tgaacctaaa ccacagccaa
    20761 cacccaagca gcctaaaaaa accgcatggg aacctgatga aattatttgg aaaaaagtga
    20821 ttgccgcgtt atcgttaaag aaccgtccta ataaaaccaa aactttacgc aatacaatcc
    20881 aggcactcac aaaatccaat gcacaagaaa ctgacaaact gctacaacat ttacaaggta
    20941 cccaagtcct acgtattgac ggtactaaaa ttgtgtatat caagtaggat gggcatttat
    21001 gcctgaccta caaaaccaaa aacaacctaa cacccttaat caactccaca aacccttttc
    21061 agacaacctc gttttttgaa aaacaatctg taaacagata actgctgaag aataccgttg
    21121 ccgagcccca aaacctgcct gcaactttta ttgtgaactt cccattatga aaaaatccct
    21181 tttcgttctc tttctctatt catctctact taccgccagc gaaatcgcct atcgctttgt
    21241 attcggaatt gaaaccttac cggctgcaaa aatggcagaa acgtttgcgc tgacatttat
    21301 gattgctgcg ctgtatctgt ttgcgcgtta taaggcttcg cggctgctga ttgcggtgtt
    21361 tttcgcgttc agcattattg ccaacaatgt acattatgcg gtttatcaaa gttggatgac
    21421 gggcatcaat tattggctga tgctgaaaga gattaccgaa gtcggcagtg cgggcgcgtc
    21481 gatgttggat aagttgtggc tgcctgcgtt gtggggcgtg ttggaagtca tgttgttttg
    21541 cagccttgcc aagttccgcc gtaagacgca tttttctgcc gatatactgt ttgccttcct
    21601 aatgctgatg attttcgtgc gttcgttcga cacgaaacaa gagcacggta tttcgcccaa
    21661 accgacatac agccgcatca aagccaatta tttcagcttc ggttattttg tcggacgcgt
    21721 attgccgtat cagttatttg atttaagcaa aatccctgtg ttcaaacagc ctgctccaag
    21781 caaaatcggg cagggcagtg ttcaaaatat cgtcctgatt atgggcgaaa gcgaaagcgc
    21841 ggcgcatttg aaattgtttg gctacgggcg cgaaacttcg ccgtttttaa cccagctgtc
    21901 gcaagccgat tttaagccga ttgtgaaaca aagttattcc gcaggcttta tgacggcagt
    21961 atccctgccc agtttcttta acgtcatacc gcatgccaac ggcttggaac aaatcagcgg
    22021 cggcgatacc aatatgttcc gcctcgccaa agagcagggc tatgagacgt atttttacag
    22081 tgctcaggct gaaaaccaaa tggcaatttt gaacttaatc ggtaagaaat ggatagacca
    22141 tctgattcaa ccgacgcagc ttggctacgg caacggcgac aatatgcccg atgagaagct
    22201 gctgccgctg ttcgacaaaa tcaatttgca gcagggcagg cattttatcg tgttgcacca
    22261 acgcggttcg cacgccccat acggcgcatt gttgcagcct caagataaag tattcggcga
    22321 agccgatatt gtggataagt acgacaacac catccacaaa accgaccaaa tgattcaaac
    22381 agtattcgag cagctgcaaa agcagcctga cggcaactgg ctgtttgcct atacctccga
    22441 tcatggccag tatgttcgcc aagatatcta caatcaaggc acggtgcagc ccgacagcta
    22501 tattgtgccg ctggtgttgt acagcccgaa taaggccgtg caacaggctg ccaaccaggc
    22561 ttttgcacct tgcgagattg ccttccatca gcagctttca acgttcctga ttcacacgtt
    22621 gggctacgat atgccggttt caggttgtcg cgaaggctcg gtaacgggca acctgattac
    22681 gggtgatgca ggcagcttga gcattcgcga tggcaaggcg gaatatgttt atccgcaatg
    22741 agtggcgtaa aaaccaataa agacaaattt agatgatgtc ggggaagatg cccgaccgac
    22801 aagactatgc aaaatatgaa aaaccaagta cgcggatcag gcatggatgc ccgatccaac
    22861 ccggccaatg tttcagacgg cctgcaaaac agttcgggtc atatcggtac caacacgcgt
    22921 taccgcctga ccaaactcgg cgaacaaatg gcgcgcctgc ccatcgaccc gaaaatcgcg
    22981 cgcattttgt tggcggcgaa gaagcacgac tgcatggcgg aaatattggt gatcgcgtcc
    23041 gcgctgtcga ttcaagaccc gcgcgagcgg ccgctggaag cgcgtgatgc cgcggccaag
    23101 gcgcacgagc gttttaccga caagcaatct gactttcttg cctatctgaa tatttgggac
    23161 agctttcagc gcgagcgcga caaaggtttg tccaacaagc agctggtgca gtggtgccgc
    23221 caatattttc tgtcgcacct gcgtatgtgc gaatggcgcg agctgcacca ccagcttgcc
    23281 caaaccgcga ttgaaatggg tttgaccacc aaggaaaccg ctttcagacg acctcccgaa
    23341 atcaggcagc tcacgtcgtc tgaaaatgcg ggtgaccaag acttatccgc caagctcaaa
    23401 caaaaacaac tggataaaaa gcaacaccgc gcccaaatcc gcgccgccaa agaagcgggc
    23461 tacgaacaaa tccaccgcgc cctgctgacc ggccttatcg ccaacgtcgg catgaaatcg
    23521 cccgacggca acgactacac cggcgcgcgc ggcagccgct tccacctttt ccccgcctcc
    23581 gccctgttca aagccaaacc caaatgggtg atggcggcag aactcgttga aacgacaaaa
    23641 ctttatgcgc gcgacgtcgc cgttatccag cccgaatgga ttgagcagga agccccgcac
    23701 ctcgtccgct accactattt cgagccgcac tgggaacaaa aacgcggcga agtcgtcgcc
    23761 agcgaacgcg tgacgcttta cggcctgacc gtattgccgc gccgccccgt gccttacggc
    23821 aaagttgcgc ccgaagaagc acgtgaaatc tttatccgca gcgcgttggt ggcgcaggaa
    23881 tgcgatttga aagcggattt ttttgtccac aataaaaagc tgattaaaga aattaccgaa
    23941 ctcgaacaca aatcgcgcaa gcaagacgta ttggtcgacg acgaagccct gtttgcgttt
    24001 tataacgaac gcctgcccga aatggcttgg aaagacgcgc aaggcagcgt ttggggaagt
    24061 gaagattccg tacggattat tgaatctgac aaagccgaga ggtcgtctga aaatgagcgc
    24121 aacgagtttc gtaaaaacaa gcgtaatggg tctcgccaaa atgaaaatca cggcaacacc
    24181 gtaggttggg ttgaaaaccc aacatcagcc gcaactgcaa aaactgttgg gtttgacaac
    24241 ccaacctacg ctactcagca acccaccccc tcccccgcga gggagggtcg gggagagggc
    24301 aaaacagttg ccgcacaaac caacttttcc gcaaccgcag caaaccctct cccgcaggag
    24361 agggagcaga gtgccgcagc ttcagcggtt tcaaacgacc tgcatcctgc aaatccccaa
    24421 caaaccgccc cctcccccgt gggggagggt cgggaagagg gcaaaacagt tgcctctcaa
    24481 accaacttct ccgcaaccac agcaaaccct ctccctaacc ctctcccaca ggagggggaa
    24541 cagagtgccg cagcttcagc ggtttcaaac gaccctcaac cccaaaagca gccagcaccc
    24601 caaaaagacc gtctgaaacc cctccccctc gccgacatcc gcaccttcca agcctggctc
    24661 aaaaccgccg aacgcgagaa cccgcgcctg ctgttcctca gccgcgacga tttgatgcaa
    24721 cacgccgccg cacacattac cgaagagcaa ttccccaaat tctggcaaac cgcagacggc
    24781 aagttcaaac tttcctaccg cttcgagccg caccatccgc tcgacggcgt taccctcacc
    24841 ctaccgctga ccgtcctcaa ccgcctgcac gcgccgtcgc tcgaatggct ggtgcccggt
    24901 atgttgcgcg aaaaaatcca gctgctgatt aaagcactgc ccaagcaaat ccgccgcatt
    24961 tgcgtgcccg tacccgattt catcaccaaa tttctcgaaa gcaatcccga ccgccaagcg
    25021 gtcattattc cccagttggc gcactttatc gccaaaagcg caggcgatat gcggattttc
    25081 gagcaaatcg accaagacgc atgggcggcg caagaattgc ccgaacactg ctatctgaat
    25141 ctgctcatca tcgacgacgg cggacaagag cttgccggcg gccgcaaact gcacgaattg
    25201 caacaacagc tcggccaagc cgccgccgtc accttccgcg acaacaccca agaattcgag
    25261 cgcgacaacg ttaccacatg ggatatcggt accctgcccg aatccatcaa attcgcccgc
    25321 ggcaaacaac agctcaccgg ctacctcggt ctgcaaaaag aaaaagacgg ccgcatcgcc
    25381 ctgcgcctgt gtgacaccac cgaagctgcc gaacaggcac accgtcaagg cgtcatcgaa
    25441 ttgatgaagc tgcaattaaa agagcaggta aaagacctga acaaaggcat ccaaggcttt
    25501 acccaagccg ccatgctgct caaacacatc aacgccgaca ccctgcgcga cgacctcacc
    25561 caagccgttt gtgaccgcgc ctttatcggc gaagacgagc tgccgcgcaa cgaaaaagcc
    25621 ttcaaagaac aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agccctcagc
    25681 cgctacctgc aggaaaccgc cgccgcctac gccgaactga acggcaaact cggcaaacac
    25741 ccattgaccc accttctaag actacgcctg caaaccctgc tcgccgccgg cttcgccacc
    25801 cgcaccccgt gggcacaatg gccgcgcctc cccatctacc tcaaagccat gaccctgcgc
    25861 ctcgaaaaat acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatacaggaa
    25921 ttggaacaaa tgtggcagga aaaaaacgac ggtttggtga aacaaggact acccgtttca
    25981 gacgacctca ccgcgtttaa atggatgatc gaagaattga gggtgtcgct gttcgcgcag
    26041 gaattgaaga caccgtatcc ggtgtcggtg aagcggctgt tgaaagagtg ggaggatctt
    26101 aattaaagga agatgccgtc taattttttc agacggcatc ttttatttcc ctgaccaacc
    26161 gatttaatcg ctccctacaa atagctttat tttttaattc gcactcccaa acgacaatca
    26221 ctttgaaacc gatcttttcc agctcggttt catttttaat atcacgttcg caattttttg
    26281 tgattttttc caaccaaaaa tccgtattgc ttttcgggat atgtccttta ttacaagaat
    26341 gcccgtacca aaaacagccg tgtataaaca cgactgtttt atatttcgac aaaacgatat
    26401 ccggtttgcc tacatagcgt ttatcgtttt tccgataacg gaaaccttgg aaaaaaagaa
    26461 atttccttac caatacttcc ggtttggttt ctttgctatg gatttttgac atcacgaaag
    26521 aacgtttgga tgtagtgaaa atatcggtca taaattactt ggaagatacc accttcgatc
    26581 gtttttttgt ctgttccact tttcttaaca aatacgaaac caagcttatt aaatccaaag
    26641 catccttatc attaaaaata ctaggagata aatttgctct taactcatgt tgtacaggat
    26701 ttctaaatcc agtccatgtt ccgcatgcaa gctgttcaag accatcttct aaattttgtt
    26761 ccgccttcgt tttgttattc gttaatagaa gtatagaatt tttactcccg aaagcttgtt
    26821 caatcaattc tcttccgtct atgctatgaa gacctgtttg cttctgaacc ttatcttcga
    26881 aaactttaac tgcctcgatt gcagcactta aataatcctt cttttcattg taaaaatcat
    26941 tacaggcagt atgcaaatcc tgatgcaaat gccgccaatg atagtaagga tattctggaa
    27001 taatactatg taaggcagag attacttccg cttgctcttt atttgtaagc tccgaatctt
    27061 ccaaacgatt caaaataact tctactggtt ccctttcctt ttctggcaat ttgtttttcc
    27121 aatcttcgat atcaactccg ctttcgcctt tcacttcgtc atctttctct ttttttcttc
    27181 tttttctcca atcttgcccg atggctcgga cgatctcccc aaggaaaaca cgtaattttg
    27241 aggtttttga atgcttccag tctaatgatg tcctgtcggt agcaattaaa tcttcatcgt
    27301 cattatctat aaagtccaca ttcagccatc cagtcaaata tgaatagaaa tggctggatt
    27361 cgctatcagt gaaaaactcg ggcatattta ccattctgcc gttggcaaac aaggtaatac
    27421 ctttcatatt attctttaaa ggtttttccg ttgtaatgaa cttaccttgt attaatccgc
    27481 tgtattcata ctgttcgaat tttgaagata acgatgaaat attggttgct aaatcctggt
    27541 attcccattc gaattgtggg gtaacaagat catattttag atttccatca atgattttag
    27601 gttcagagcc gttcaagctt actttgattt taaaatcctg agccggaaaa tcaaacaagc
    27661 gggaaagatg ctctacataa ttatctaacg gatatccttg ctttttcgtc agttcggtta
    27721 aagttatggt tgtcccgctt tcgatattgg attcaacaga ttcttttcga aactccggtt
    27781 gataaatacc cttgcttctt cgaatctctg cataatccaa agtaaaagta accctttcgt
    27841 ttccctggat agtagaaatt tcaattttgt tgccaagccc gaataatgcc aatttaccaa
    27901 ggcctttttt acccgttgga attcttccgc acggggaggc ttgtttttct tcccttctgt
    27961 tccgaccgat tctcaaataa aaatcattga tttcatcgaa gctcattcct ataccattat
    28021 ctttaataac gattttatgc tccggtttat cgaataacct aacttccact tccgtagcac
    28081 atgcatcgta ggcattcgct atcaactcgg caatcgcagg aacagtgtgc gaatacatct
    28141 ttacacccaa atgttcgatt acggttggct cgaaactcat tttcaatttt tcttcagaca
    28201 tgttttctcc ttttctaaca ttcgatatta tcaacaattg ccctgcctat tttttccgca
    28261 tacatcggag gaacggcatt accaatcaaa cgggctatct tgtccctgct gcccgcttta
    28321 aagacatagt tacgagggaa cgattgcaat gttgcacctt cgcgcagtga taacgcccta
    28381 tcttcttcag gatgggcaaa cctcccgttg gaaatactaa agaatttggt cgttatggta
    28441 ggtgcgggct tatcccacca tagcctacca aacgtgtctt taaaactatt gtcttttccg
    28501 ataaaacatt ccagctgtaa ttcgggaata tgcgcaaaag ccaaacggtt tcctccgttt
    28561 ttaggtatca aagccaatcg tttcaagttg atatcagata atcccgcaca gctatgcata
    28621 aaatccgttt cgtcttggtg tcctgcaata attttgggaa agccattttc cattcccaaa
    28681 acatcgcgta ccgtaagccg tttgcccgaa tacttgactg gttccagctt ttctttggtt
    28741 attctgtttg caattaacgt aaatctttta cggctttggg gaattccgta atcagccgta
    28801 ttgtgaatac cgaaatggac ggtaaatcct ttttcttcca ataactttat gaaattatca
    28861 agcctgcttt ctttcattct gctcagaatt cccggtacgt tctctacaac aacatatccg
    28921 ggattgaaat attctacaaa ccgctgaaac tccaaaagca aactcttgga tttttccgat
    28981 tttcttttgt ctgtttgaat aacactccaa tattgacaag gactacaacc tataagaatc
    29041 aaatcatcat cattcttttt taacccgagt tctttttcca aggtttcagg ctgcaattca
    29101 aaaacatcag cctgtataaa tttggcattt atattggctt catatgtttc tttgcagctg
    29161 ggttcgtaat ctatcccggc taaaacctga ataccggcgg attgcattcc atagctcatt
    29221 ccacctccgc cacagaaaaa atcaaccgcc tttaacttac tgtttttcat ttctatttaa
    29281 ataattagac tagtgagaac taactttata taacatataa tttattgctt atcaaaaata
    29341 atttgagatt ttattaaaat tcatgcatct aaataagcag aacttaattt cctccaaacg
    29401 agcggatgtc ttcaaatgca cacttcacag aaaggtcgtc tgaaacccat tttcagacga
    29461 cctttttcca cttcccaaaa caagaattcc caccccctcc ccaaacgtcg cccgaaccag
    29521 taaactcccc atatttcaac aacttcttgg aagccaacca tgccccgtat ccaccctcct
    29581 cacctgttcc cgccccatat cgccgaacgc ggccttttgt attttcagca gggcaaggtt
    29641 cgcgatgtcc gaaaaacttc cgccgggcgt tatcgggcgg aggtatgcgg tttggaaaac
    29701 tattgggtat ggctgaagct ggatagtgat ttgtatatta aagacgaaag ctgcgattgc
    29761 ccttatgtct aaaggtgcaa acatacctta aattactata ttacataggc aaaatacaag
    29821 cctataacga attggaaaca aaatgccgtc tgaaaacatc ttcagacggc attataaaat
    29881 ctgttcacct tttcagatga gtaatgtaca cccctataca atttttgcta ctatacccca
    29941 taaatccacg gctaaagata tccttattat gtcctatgat ttatcgaaac gacttgtaat
    30001 cggcttagca tcaagtgccc tattcgactt atccgaatcg gataatatat ttagaatgga
    30061 aggggcagaa acctataggc aatatcagag agaaaaacaa aaccatcccc taaaaaaggc
    30121 gttgtctttc catttattaa aaaacttctg tcaatcaatg aaataaaccc aaacgaccca
    30181 acgattgggt ttattctttt atccagaaac aatccagata cagattacga gtcataacta
    30241 taggcttaat attacacgat tttcattcca tcaaggcgga aaaccgcaca aatactgaaa
    30301 cactatcgat cgatttgtaa acaagcctac ttaagtaact tgcagtcctt atcatttcct
    30361 ttaaaataat ccagcccgtc actacacgaa ctggcggact tcttgcaaat aaaggttact
    30421 agattttcat tcatcttaat aataaaagga tttttatctt tatctatggc taccgccttc
    30481 aacatgaatt tactgtctaa ggccccgcgc gcgattccat tcaaacggat acaaaagcct
    30541 tctgcctctt taatcggcaa acttggccat ttggtagatg tttgtttaaa tctcccattc
    30601 tgcagataaa acttttccat aaaatgtgca ttttctaaca aggctgcccg cactgtattt
    30661 atctttgctt tctcaacata attgcgatag ctcggataaa caattaaagc aagtacagac
    30721 aatatcaaga ccactgatat taattcaacc agcgtaaacc cccgattatc agtcattact
    30781 ttacttccaa taagaacaga ttattcaaca tatttctttg aacagactta ctatcccatt
    30841 cagcagtatg catatttccc act
//
LOCUS       DAWFKN010000016        30441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFKN010000016 DAWFKN010000000
VERSION     DAWFKN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.016"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR46_000726"
     CDS             complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR46_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0855711.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2317..2589)
                     /locus_tag="WGR46_000727"
     CDS             complement(2317..2589)
                     /locus_tag="WGR46_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855712.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2775..3461
                     /locus_tag="WGR46_000728"
     CDS             2775..3461
                     /locus_tag="WGR46_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0855713.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3593..3931
                     /gene="erpA"
                     /locus_tag="WGR46_000729"
     CDS             3593..3931
                     /gene="erpA"
                     /locus_tag="WGR46_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0855714.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3948..4094
                     /locus_tag="WGR46_000730"
     CDS             3948..4094
                     /locus_tag="WGR46_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855715.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4091..4555
                     /locus_tag="WGR46_000731"
     CDS             4091..4555
                     /locus_tag="WGR46_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0855716.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPELPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR46_000732"
     CDS             4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR46_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0855717.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR46_000733"
     CDS             complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR46_000733"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0855718.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7460..8038
                     /gene="grpE"
                     /locus_tag="WGR46_000734"
     CDS             7460..8038
                     /gene="grpE"
                     /locus_tag="WGR46_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0855719.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8062..8202)
                     /locus_tag="WGR46_000735"
     CDS             complement(8062..8202)
                     /locus_tag="WGR46_000735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855720.1"
                     /translation="MKKNRQAAYGLTVNAETKCRLKPDRRVQTALPLYFDRFIRKTRF
                     RK"
     gene            complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR46_000736"
     CDS             complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR46_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0855721.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8403..9458)
                     /locus_tag="WGR46_000737"
     CDS             complement(8403..9458)
                     /locus_tag="WGR46_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0855722.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVHLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR46_000738"
     CDS             complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR46_000738"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0855723.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR46_000739"
     CDS             complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR46_000739"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0855724.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(11439..12065)
                     /locus_tag="WGR46_000740"
     CDS             complement(11439..12065)
                     /locus_tag="WGR46_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0855725.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(12065..12841)
                     /locus_tag="WGR46_000741"
     CDS             complement(12065..12841)
                     /locus_tag="WGR46_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0855726.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(12834..14066)
                     /locus_tag="WGR46_000742"
     CDS             complement(12834..14066)
                     /locus_tag="WGR46_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0855727.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(14069..15412)
                     /locus_tag="WGR46_000743"
     CDS             complement(14069..15412)
                     /locus_tag="WGR46_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0855728.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(15760..17181)
                     /locus_tag="WGR46_000744"
     CDS             complement(15760..17181)
                     /locus_tag="WGR46_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0855729.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(17539..17898)
                     /locus_tag="WGR46_000745"
     CDS             complement(17539..17898)
                     /locus_tag="WGR46_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0855730.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(17895..18500)
                     /locus_tag="WGR46_000746"
     CDS             complement(17895..18500)
                     /locus_tag="WGR46_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0855731.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(18547..19029)
                     /locus_tag="WGR46_000747"
     CDS             complement(18547..19029)
                     /locus_tag="WGR46_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0855732.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      19233..19330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19541..20641
                     /gene="gcvT"
                     /locus_tag="WGR46_000748"
     CDS             19541..20641
                     /gene="gcvT"
                     /locus_tag="WGR46_000748"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0855733.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            20804..21190
                     /gene="gcvH"
                     /locus_tag="WGR46_000749"
     CDS             20804..21190
                     /gene="gcvH"
                     /locus_tag="WGR46_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0855734.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            21342..22589
                     /locus_tag="WGR46_000750"
     CDS             21342..22589
                     /locus_tag="WGR46_000750"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0855735.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            22865..23290
                     /locus_tag="WGR46_000751"
                     /pseudo
     CDS             22865..23290
                     /locus_tag="WGR46_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            23296..24526
                     /locus_tag="WGR46_000752"
                     /pseudo
     CDS             23296..24526
                     /locus_tag="WGR46_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            24584..25204
                     /locus_tag="WGR46_000753"
     CDS             24584..25204
                     /locus_tag="WGR46_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855736.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            25215..25346
                     /locus_tag="WGR46_000754"
                     /pseudo
     CDS             25215..25346
                     /locus_tag="WGR46_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            25343..25837
                     /locus_tag="WGR46_000755"
     CDS             25343..25837
                     /locus_tag="WGR46_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0855737.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(25971..27632)
                     /locus_tag="WGR46_000756"
     CDS             complement(25971..27632)
                     /locus_tag="WGR46_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0855738.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(27762..28305)
                     /locus_tag="WGR46_000757"
                     /pseudo
     CDS             complement(27762..28305)
                     /locus_tag="WGR46_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(29239..29892)
                     /locus_tag="WGR46_000758"
     CDS             complement(29239..29892)
                     /locus_tag="WGR46_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0855739.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSGFIHYRINHSKECADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT         7325 a         7407 c         8149 g         7560 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatataa aaaatttcca gagaaccgat acaacagttg
       61 gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat gctatggtct
      121 tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag acggcatcaa
      181 ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat cgtctttctt
      241 tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg cttgagcgta
      301 aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg catcgatggc
      361 ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt cgattttttc
      421 tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca cggagtgaat
      481 cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat cttcctcagc
      541 attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac ctgaagaacc
      601 ttggatggta atgttggctg ctttaccggt gcctttgtct ttggcggaaa cgtgcaggat
      661 gccgttggcg tcgatgtcga aggttacttc gatttgcggc ataccgcgcg gtgcaggtgc
      721 gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt cgcgttcgcc
      781 ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga acacttgcga
      841 cgctttagtc gggatagttg tgttcttttg aatcagcttg gtcatcacgc cgcccatggt
      901 ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc ggccgccgct
      961 taacacttcg ccttgaatcg ctgcgcctac ggcgacagct tcgtcagggt tcacgtcttt
     1021 gcgcggctct ttgccgaaga agtctttaac ggcttcttgt actttcggca tacggctttg
     1081 accaccgacc aaaatcacgt cgtcgatgtc gccagtgctc agaccggcat ctttcaatgc
     1141 ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt cgaatttggc
     1201 gcgggtaatt ttcatcgcca agtgtttcgg gccggttgcg tccatggtga tgtacggcag
     1261 gttaatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg cagcttcttt
     1321 caggcgttgt agagccatca cgtcttgttt caaatcaatg ccttgttctt ttttgaactc
     1381 ggcgatgatg tggtcgatga ggcgttggtc gaagtcttca ccgcccaaga aggtatcgcc
     1441 gttggttgcc aatacttcga attgtttgtc gccgtcgagg ttggcgattt cgatgatgga
     1501 aatatcaaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt tgttgtcgcc
     1561 tttgtccata ccgaatgcca aagcagctgc agtcggctcg ttgatgatgc gtttcacgtc
     1621 caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt tgaagtaggc
     1681 agggacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg cggcttcttt
     1741 cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc cttgtgcttt
     1801 tacccatgcg tcgccgttgt tggctttgat gatttcgaaa ggcatagatt cgatgtcgcg
     1861 ttggacttct ttgtcttcaa atttgtggcc gatcaggcgt ttggcggcgt aaatggtgtt
     1921 tttagcgttg gttacggctt ggcgtttggc aggcgcaccg acgaggattt cgccgccgtc
     1981 caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga tcactttggt
     2041 ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga taccgattac
     2101 ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg ttggaacatt
     2161 ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac agcatggcta
     2221 taagtatata actttatgaa tatattggtt ttatatttga tttaatacat ttggctccaa
     2281 tgcatttaag cataatattt caaatggcag gcaggtttat tcatagacga tgccggcgag
     2341 catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag actcgatgat
     2401 gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt tcagtatgtt
     2461 gaatttttcg cacagggatg cggataaagt gatgtcaggc tgtttggctt cagaacggtt
     2521 ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat atttgccagt
     2581 tttcttcatt ttctatcctt tttctgtcaa ctcaggatta aacctatgga aaatcttaaa
     2641 aattatgtat tgagtaagaa aaatcataat ttaaatttag tttatcatag ttattccgtt
     2701 tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga atgaagggga
     2761 gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg aaaagcgaag
     2821 cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc agcaggatat
     2881 ggaatgaagg cggtctgccg aagtctgaaa cattaaaaaa aatcaagcag ttgaaggggt
     2941 gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa gccccaaaaa
     3001 aatcccttgc ttacgatacc ttgggaaatg aagtcgatac ggacgagttt gtctttgtgc
     3061 cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat gaggaaccgg
     3121 tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc gatacgaaaa
     3181 acctgtctgt aatttccgtc aagggggatt cgatggaggg ggttttgaat gacggcgatt
     3241 cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg ttgcggatta
     3301 atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc aatgtgattt
     3361 ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc gatgatgtgg
     3421 agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc ttgaaattgc
     3481 aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc tgcttgcctt
     3541 tgaagggggt ttgagagaga atgcagaaaa tattatatta aggaataaca ccatgtcgga
     3601 tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt tgattgccga
     3661 agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct gttcgggttt
     3721 ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa ttgagaaaaa
     3781 cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg cggaaatcga
     3841 ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg cggaaacaac
     3901 ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg ccgtctgaac
     3961 gttcagacgg catttttact tttagaaaat atattatcgg gatgaattca catataatcc
     4021 gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta tcaggcgtat
     4081 ttgttccctt atgattgaga tgagtaaaga ttaccgaaac gatttgtacg atgtatatgt
     4141 ttcttacccg ccccaagtgg atcgcgggct tatccgggag tgccttaagg agaatctcgg
     4201 cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag tgctggttga
     4261 ggaaaaatgc acttgggcga aacgggaaga gttgcatgat tatttcagct atttgggttt
     4321 ggatattatt acccggagat atatggagtt agaaacggtc gtgccgccgg aggaaggtga
     4381 gggcgaagga gagggcgcgg atggggaaat gcccgaatat cttgaacttc atggcgggcg
     4441 ggaagatgat atttccgcac cttcgcaacc cgaactgccg acccgcaata tcaaaccgct
     4501 gcttttcggg ctgctgattg cctttttggg ctatctgctc ggtaagattt tttgattgtc
     4561 cgataaatgc tgtattcggg attttatata tgaaatggtt gaaacgcctg acggtcattg
     4621 tcgggacttt ttaccgctat cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt
     4681 ggatatgcgc tctgctgaaa atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg
     4741 ccgtccgtct gcgccttgcc ttggaaagcc tggggccgat tttcatcaag ttcgggcagg
     4801 ttttgtccac acgccccgat ttgattccgc acgattacgc cgtcgaactg gcaaagctgc
     4861 aagacaaagt gccgcctttt gacgcgcggc tttcgcgtga acaaatcgaa aaatcgttgg
     4921 gtcagtccat cgaaaagctg tatgcggaat ttgaaaccga gcccatcgcc agcgcgtcca
     4981 tcgcccaagt acacaaagcc cgcctgcatt cgggcgaacg agtggcggtt aaagttttgc
     5041 gccccaacct tttgcccctt atcgaacagg atttgtcgct gatgcgtttc ggcgcggcct
     5101 gggtcgaacg cttgttttca gacggcaggc gtctgaagcc gctcgaagtg gtggcggagt
     5161 tcgacaaata cctgcacgac gagttggatt tgatgcgcga agccgccaat gccggacagc
     5221 tcgggcgcaa tttccacaac agcaatatgc tgattgtgcc caaggtgttt tacgactact
     5281 gcaccagcga cgtgctgacg attgaatgga tggacggtac gccggttgcc gacatcgcca
     5341 aactcaaagc agacggcatc gatttgcaca aactcgccga ttacggcgtg gaaatctttt
     5401 tcacgcaggt cttccgcgac ggctttttcc acgcggatat gcaccccggc aatattttgg
     5461 ttgccgccga caaccgctac atcgccctcg atttcggcat cgtcggcacg ctgaccgatt
     5521 acgacaaacg ttatctcgcc atcaatttcc tcgccttttt caaccgcgat taccggcgcg
     5581 tcgccaccgc ccacatcgaa tcgggctggg tgcctgccga cacgcgtgcg gaagaattgg
     5641 aagccgccgt ccgcgccgtg tgcgaaccgg tgttcaacaa accgatttcg cagatttcct
     5701 tcggcttggt gctgatgcgc ctgtttgaag tcagccgccg cttcaatgtc gaaatccaac
     5761 cgcagcttgt gctgctgcaa aaaacgctgc tcaacatcga aggcttgggc cgccagctcg
     5821 atcccgattt ggacttgtgg aaaaccgcca aaccgttttt ggtgaaatgg atgaacgggc
     5881 aggtcggccc taaagccctt tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa
     5941 tcattccttc attgccgcgc aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa
     6001 tgcgtgatgc ctatatccat ttggtcaaag tgcagcagcg gcaaagcctg tggctggctg
     6061 tgattgcggt tgttttgctg ctgattttgc ttttgaaata ggctttgtcc gaatcatcgc
     6121 ccgactccgc ccgtttataa ggaaatcggt tatagtggat taacaaaaac cagtacggcg
     6181 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     6241 attccgtact atccgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     6301 ctatatttcc ggttgcgtgg gaatcgggtg tatcgaataa aaggcatttt gtccgactgg
     6361 caagtgccga catcggcggc atatcaaggc gcaggcttga agcgggcaat gtcgtctgaa
     6421 gcccgtttgg cgtttcagac ggcattggtg cggatattca aatcataaag tcgatttcgg
     6481 taaactggat attttgatcc atatccgccg acggtgtttt gagcgatcgc gccacgggtt
     6541 tggcgggtac gccgacaacc gtgatggacg gcggcacgtc tgaaaccacg acgctgcccg
     6601 ccccgatttt ggcattgctg ccgatgcgga tattgcccaa tatcgaggcg tttgcgccga
     6661 tcatcacgcc gtcgccgatt ttagggtggc ggtcgccgcc ttctttgccc gaaccgccga
     6721 gcgttacgcc gtgcaaaatc gaaatattgt tgcccaacac ggcggtttcg ccggcaacaa
     6781 agccggtggc gtggtcgagc atcagcccgt atccgaaacg ggcggcggga tggatgtcca
     6841 cgccgaatac ttcggacata cggttttgca ggaaatacgc cagcgttttg cgcccgtcga
     6901 gatacagccg atggttgatg cggtgtgcct gaatcgcgtg gaagcctttg aaatataaaa
     6961 gcggcagcga atattcgtcg caggcgggat cgcgttcgta gatggctttt aagtctgctt
     7021 cgacgcattt gccgatttgg gtgtcgctgc ccaacgcctg ctggtagatt tcaaacagcg
     7081 cgcgcacgtc cataatcggg ctgccgagtt tgctggaaag gtggtaggca aggacggagc
     7141 cgagggactc gtggcgcaac acggtttggt gcaaaaaact tgccagcatc ggttcggcgg
     7201 agaccgcggc cgcggtttct tcgcggatgg tgtgccagag gtcgaaaccg gttgtgttta
     7261 aatggtcttt tttcatgagt gatgacgttt gaaaatcgat atggtcggca gtatcttacc
     7321 gtctatatta ttttttcggt aggggatttg aaaatgaatt tgaaattctc tgcctttgct
     7381 tgaagtttct tgaaaatgtc cttatcttgc gcgggtaata actggatttt gatttccaat
     7441 ttgttttaag ggatacgata tgagcgaaca gacacagcag caaaacagtg aagaagcggt
     7501 tgaaaatgtg gaggcggtgg aaaccgtcga gacagtagga aatgcggacg gtgtgcagga
     7561 acaggctgcc gcagagccgg cttatgagga tttgcaggcg cggattgccg agctggaagc
     7621 gcagttgaaa gacgagcagc tgcgcgcttt ggcaaacgag caaaacctgc gccgccgcca
     7681 ccagcaggaa attgcggata cgcacaagtt cgccggacag aagtttgccg tggaaatgct
     7741 gccggtcaag gattatctgg aaatggcgct tttggatcag agcggcaatt tcgatgcgct
     7801 gaaaatgggc gtgcagatga ctttgaacga gttgcagaaa gcatttgatg ctacgcaaat
     7861 caaggaaatc aatcctaaag cgggcgataa gctcgatccg aatatccatc aggcgatgca
     7921 ggcggtggca agcgaacagg aacccaatac cgtggtgggc gtgatgaaga agggttatac
     7981 gctgtccgac cgcgtgttgc gcccggctat ggttacggtg gcacagaagg aagcctgaag
     8041 gcgtctgggg aataatctga tttatttcct gaagcgcgtt ttgcgtataa accgatcgaa
     8101 gtaaagcggc aatgccgtct gaacccgcct gtcgggcttc agacggcatt ttgtttcggc
     8161 gttaacggtc aacccgtatg ccgcctgcct gtttttcttc atccagtttc ttttgcaggg
     8221 tgtcgcaagg tgtgtcgcag tcgcacattt ttttcatacc caaggcagta atgccgccgc
     8281 aactgccttt gatgctgcgt ttggagaaaa tatagccgac cgccataccg atgatgacgg
     8341 tcaggaagat gccgaaggta aggagcaggg ttttcatggt gtttcctaat cggtttgtat
     8401 gtttagcgga gcagtttttc aaattcggaa gacatggcgg tgcggtagcc gcctttatcc
     8461 ctgacaatca ggaaaacagc gagtttttcg cgctctgcca gctttaaggc ttcggtttcg
     8521 cccaatacga ataatcctgt ggacaagccg tccgccgtca tcgcactgtc tgcgaccacg
     8581 ctgatggagg cgaggttgtg gctgatgggt cgtttgttgt tcggattaat gatatgggag
     8641 aggcgtttgc cgcttttatc gacgtggaaa atacggtaat cgccggaagt ggcaagcgaa
     8701 cggttgttca gcgggacgat aatctgcgta ttgccgcctt ggacgatgtt gggctgttcg
     8761 atgccgatgc gccaaggttc gccgcgcgcg tttttgcctt tgccgtgcaa ctcgccgccg
     8821 atttcgacca gataattttg aatgccgtat ttttccagtt cgcccgcaac tttatcaacg
     8881 ccgaagcctt tggcaatcga agataaatcc aaataggcct tggggtgggt tttgctcaag
     8941 gaagcgtaat ctttgccttg tttcaaaatg attttgtcta tgcccgtata agatgctgct
     9001 tgtttgattt gttccggcga cggttcacgg gtaacggatt tgtcggggcc gaatccccaa
     9061 aggttgacca aggggccgac ggttacgtcc agcgcgccgt gtgtcaggcg gttcaggtgg
     9121 acggcttcgg cagtaacgtg tgcgaagtcg cttgaaatgc ggaggggctt gccggctgtg
     9181 tgttggttga accggctgat ttcggagtcg ggctgatagg tggacatctg ccggttgact
     9241 tctttaagcg cgtcatcgat gcgcttttgt atttcggcag gtgaggggag tttgtcccga
     9301 ttatttgaaa ggtatttgac ggtataggtc gtgcccatcg tttcaccttg cagggtaacg
     9361 gtttgcgcgg tttgttccga acaggcgttc aggaagataa aactcagggc aaatatcaag
     9421 gtgcggataa agttcggcag gcgtgtttca gacggcatag tgtttgacgg ttttggcaaa
     9481 tggtttgaat tatatcgcaa aacggccggt atgtttctat gccgatgccg tctgaagggt
     9541 gttcggatgg catcggcata gaaaaaggaa gaaaccgagg tttcttcctt ttgtatttga
     9601 agccgaatac tcaaccgccg aaatcatcca agaggatgtt ttcgtcttcc acgcccaagt
     9661 ctttgagcat tttgatcacg gactggttca tgattggcgg accgcacata taaaattcgc
     9721 agtcttccgg tgcttcgtgg tttttcagat ggttttcgta aaccacgttg tggatgaagc
     9781 ccgtgtagcc gtcccagttg tcttccggca gcgggtcgga cagggcgacg tgccacgtga
     9841 agttcgggaa ttctgctgcg agttggtcga agtcttcgac atagaacatc tcgcgtttgg
     9901 aacgtgcgcc gtaccaaaag gtaatcttac gtttggagtt caaacgtttc aactggtcga
     9961 aaatgtggga acgcatcgga gccatacccg caccgccgcc gataaatacc atttcggcat
    10021 cggtgtcttt ggcgaaaaat tcgccgaacg ggccggaaat cgtaactttg tcgccgggtt
    10081 tgagcgacca gatgtaggac gacatttgtc ccggaggcgc atcaggtacg cgcgggggcg
    10141 gcgtggcgat acgcacgttc agcataatga tgcctttttc ttcaggatac gaagccatag
    10201 agtaggcacg caaaatcggc tcgtccactt tggaaacgta ttgccacaaa ttgtatttgt
    10261 cccagtcttc gtggtattcc tcgggaatgt cgaagtcttt gtaggcaacg gtgtgcggcg
    10321 gggcttcaat ttgaatgtag ccgccggcgc ggaaggggac ttcttcgcct tcgggaatgg
    10381 caagcttgag ttctttaatg aacgtggctt tgttatcgtt ggagatgacg gtgcattccc
    10441 attttttcac gccgaacact tcttcgggga cttcgatgtc catgtcggtt ttgacgttga
    10501 cttggcacga cagacggcag ccttcgcgtg cttcgcgttt gctgatgtgg gacagctcgg
    10561 tcggcaggat gtcgccgccg ccgcttttta cgacgacgcg gcattgtccg cacgaaccgc
    10621 ccccgccgca ggcggagggg ataaagatgc cttcgttggc aagcgcgccc aagagtttgc
    10681 cgccggcggg catcgtcagc tctttttcgc cgttgacttt gatggtgatg tcgccttcgc
    10741 tgaccagttt ggatttggca aacagaatca tcagtgccaa aaccaaaacg atgacggtaa
    10801 acatcacgat acctaaaata atctccatac cgatcccttt cttataactg gatgccagag
    10861 aacgacataa acgccatcgc catcaggccg gcggcgataa aggtaatgcc caagcctttg
    10921 aggcctttgg gagcgtccga atatttcatt ttttcggtaa tgcccgccaa agcgacaatc
    10981 gccaacatcc agcccaagcc cgcaccgaag ccgtacacga cggattcgcc gaagccgtat
    11041 tcgcgctgcg ccataaacga tacggcaccg aaaatcgcgc agtttacggt aatcagcggc
    11101 aggtagatgc ccagcgcgtt atagagggcg gggacgaatt tatccaagaa catttccaaa
    11161 atctgcacca aagcggcaat cacgccgatg aaggtgatga atttcaaaaa ggtcaaatcc
    11221 acgccttcga caatcgcgcc gtctttgagc agcgagtaaa ccaattggtt ggcggggacg
    11281 gacagtccga gtacgaaaat caccgccaca cccaaaccga atgcggtgga tacttttttg
    11341 gataccgcca aaaacgtaca catacccaaa aagaaggata gcgccatatt ttcgatgaag
    11401 acggatttga taaagaggct caaatagtgt tccatagctt attcctccgc ctgttcgggt
    11461 ttccaggtac gcagtcccca aatcaaaaag ccgatgatga agaacgcgct gggggcgagc
    11521 aggaacaagc cgttggtctg ataccagccg ccgtcctgca cggtttggaa aacggtgtag
    11581 cccaagagtt tgcccgagcc aatcagttcg cggacggtgg cgacgacaag cagcatcatc
    11641 ccgtagcccg cgccgttgcc gatgccgtcg atcaggcttt ccagcggcgg ctctttcatc
    11701 gcaaatgctt cggcgcggcc catcacgata cagttggtaa taatcagacc gacgaatacg
    11761 gaaagctgtt tggacaattc gtaggcaaat gcctgcaaga gttggtcgac cagcgtaacc
    11821 agcgacgcga taatcgccat ttgcacgatg atgcggatgc tgttggggat gtagttgcgt
    11881 accagcgaaa tgaagaagct ggaaaaaccg gttaccaaag ctacggaaat acccatcacg
    11941 atggccgtct gaagtttggt ggtaaccgcc aaagccgaac aaatacccaa aacctgcaag
    12001 gcaatcgggt tgttgtcgat aaagggtgaa aacatcaaat gtttcaagcg tttcatatca
    12061 gccattattg cgctcctgct gatttcaatt tgttcaggta ggggatatag ccgttttcgc
    12121 cgaaccagta ggcgaacgaa ccttgtacgc ctttggatgt cagcgacgcg ccggagaggg
    12181 catctacgcc gtgttctttg tccgaacccg cgcctttgcc gacgtgcagg gcgagtttgc
    12241 cttgtccgtc aaacagtttt ttgccgacga atttttgctg ccacaaagga ttgccgattt
    12301 cgccgcctaa gcccggggtt tcgccttgct cgtagtaggt aatgccgttg atggtgttgc
    12361 cgtcgggttg gatggcgaca aagccgtaca tgaccgacca caaaccgtta ccgtgcatag
    12421 gcaggatgat ttggccgatt ttgccgtctt caccttttac caagtaaacc tcggtgtatt
    12481 tggcacggct tttgatgcct gccaaatcgt cttcgggttt gatgcggatg ctttgcgcgg
    12541 ggtctttgcc tgcgatgcgc gcactgaagt ctttcggcgc gtctttcaca tattcgccgg
    12601 tcgccaaatc gaccacgcgt tgctcgatac gctcggcaaa agttttaccg atgtcggtgt
    12661 ccttatccat caaaccggct acgctcaaga tatagccttg tttgtcttgg agtttttgtt
    12721 tctcttggat gggtttcaag ccgacgaccg caccggcaac gatgaccgag caaatcaggc
    12781 tgaccgccaa cacgacaatc agcgtgccgc tgaagctgtc tttatcgaat ttcttagcca
    12841 ttgctgcgcg cctttctgcg tttgatgttc gcttgtgcga cgaaatagtc gaaaatcggg
    12901 gcaaacaggt tggcaaacag aatcgccaac atcatgcctt cggggtaagc cggattgacc
    12961 acgcggatta atacgcacat cacaccgatc agtgcgccgt accaccattt gccgacattg
    13021 gtaaaggaag cggaaacggg gtcggtcgcc ataaacagca taccgatggc gaagccgccg
    13081 acgaccaaat gccagtacca aggcatagca aacatagcgt tggtgtccga accgatgaag
    13141 ttgaacagcg aagacatggc aatcataccg atcatcacgc cggcaataat gcgccaagaa
    13201 gcgatgcggg caaacacgat aaacgcgccg ccgattaaga gtgccaaagt ggagacttcg
    13261 ccgatggagc cgggcagttt gccgataaac gcatccatcc aagtgatggt ttgaccggtt
    13321 acggcgtttt tcaggccgtc tgcaccgtgt gccgcccatt gcgccagcgc ggttgcgccg
    13381 gaatagccgt caaccgccgt ccaaaccgca tcgccgctca agttggcagg gtaggcgaag
    13441 aacaggaagg cacggcctgc cagcgcaggg ttcatgaagt ttttacctgt accgccgaat
    13501 acttctttcg caaccacaac gccgaaagaa atacccaaag ctgcctgcca cagcggcagc
    13561 gtgggcggaa cgattaaggc aaacagaatc gaggtaacaa agaaaccttc gttgatttca
    13621 tgtttgcgca cggtggcgaa caaaacttcc cagaaaccgc cgacaataaa tacggtcgcg
    13681 taaatcggca ggaagtaaat cgcgccgaac agcattttgc ccaacacgcc cgcttcagac
    13741 gacatattga tgcccaaagc gttggcaagg gcgtaatgcc agtcgttggc gatgctttgt
    13801 tgcagcaaat cgggcgttaa cgcaccgaat gcctgtgcgc cgacgttgta cataccgtag
    13861 aacatggcag ggaacaaagc cagccacacc aaaatcatca tgcgtttgga gtcgagcgca
    13921 tcgcggacgt gtgccgcttt gcgcgttacc gcaccggatg tatagaaaat cgtcgccgca
    13981 gcttcgtaga gggcatacca tttttcatgt ttgccgcccg gcaggaagtg cggttcgatt
    14041 ttttccagaa aatgtttcaa gcccataatc agccttcctt ctcaatggtt tccagcacct
    14101 tacgcaacag cgggccgtat tcgtatttgc ccgggcagac gaagctgcac aaagcgaggt
    14161 cttcttcgtc caattccaag caacccaatg cttgcgcgct gtcggtatcg ccgacgatta
    14221 aatcgcgcaa aagcagggta ggcaggatgt ctagcggcat tacgcgctcg taagtaccaa
    14281 tcggcaccat ggcgcggtcg ccaccgttga cggctgtcgt gaacttgaag agtttgtttt
    14341 tcaggaaatg gccgagggtc gtacgcgtga tggagtattt gtccggctgc ggcgcaaccc
    14401 agccgaacag ctctttgctg cggccttctt cgataacgga aatctgattg tggtagcgtc
    14461 ccaaataatc gtgcgcgcct tgtgtaatcg cgccgttcaa taccgaaccg gaaatcacgc
    14521 ggttgtctgc gtcaaccaat tcgcccgcag taatttgcga tactttcgca cccaaaacgg
    14581 tacgcaagag gcgtggtttg ttgacttgag aaccacccaa agcaatcacg cgctcggtgt
    14641 tcagacggcc tgttgcaaac aaacgtccga tggcaattac atcttgataa ttgatggtcc
    14701 aaacggtttt gtttgcaccg accggctcaa tgaaatgaat gtgcgtgcca ctcaaaccgg
    14761 ccggatgcgg gccgccgaat tcatgtgttt cgatgttggc agcattttca gacggcacgt
    14821 ctgcgccagc tgccttacac acatggattt tacgctcggt caaacggctc aataccagca
    14881 aacctcgtct gaaatcctcg gcggcttctt tgatcacaac cacagggtct gccgccagcg
    14941 gattggtgtc catcgcattg acgaagatgg cgaacggctc ggcatcgacg gcagggattt
    15001 tgctgaacgg acgggtacgc agcgcagtcc acaaaccgga ttggatcaga ttgcggcgca
    15061 cttcttcgcc gcttaagttt gccaacgctt cgggcgcgta gcgttcgaac tcgatttcgt
    15121 cgttgccttc aacggcaatc acgaccgact gaagtacgcg cttttcgccg cgatggatgg
    15181 cggcgatttt gcctgaaacc ggcgcggtaa acaccacgcc cggattcttt ttgtcttcaa
    15241 acagcacttg gccttttttg acggcatcgc cttccttgac tttcatcgag gggcgcatac
    15301 cggcatattc ttcgccaagc aacgcgactt cggtaatgac gggcccgtca taaatgactt
    15361 gctccggtct gcccgcgatg ggcaggttta gacctttttt gattttaatc atatatttgc
    15421 attacttgtg atggttaagg taaaaacggc gtgttttgat accgtgtcgc gtggcatcaa
    15481 aagcattgaa taaattaatg tagcaaagtg ttagattcta tcaggaattg tacctgtttg
    15541 tcagatttgc tgcttttttc cttgcggaag ctgtttttat agtggattaa atttaaacca
    15601 gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
    15661 ctgatttttg ttaatccact ataattgtcg gaagggggga tattgatttg attatgccgg
    15721 aatttaaaat gccgtctgaa tgttcagacg gcatagcgtt tacagcagtt tgaaaacgaa
    15781 aaagataagg gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc acatcatata
    15841 gccgaagaaa gtcggcatcg gtacgccgcg ctgttcggca atggccttga ccatgaagtt
    15901 cggtgcgttg ccgatgtagg tcagtgcgcc catgaatacc gaacccatag aaaccgccag
    15961 cagcgaatga aacagggaac ccgtcatcaa ggcttgggca tcgccgcccg ccatattgaa
    16021 aaaaacgaga taagtgggcg cgttatccaa gaatgccgac aatatgccgc tcatccaaaa
    16081 atacatcaca ttaatcggat gacctgccgt atcgtgaacc agcgatacca ccccgcccag
    16141 cgcgcctgcc tcgcctgctt tcagaatgct caggacggga aagatggtga tgaagatgcc
    16201 gaggaagagt ttgcccactt cggcgatggg ttcaaagttg aattcgttgc ctgcgcggac
    16261 ttgtttgggc gtgattgcca tagatacggc ggtcaatgca atcaggatga catcgcggac
    16321 gaggttttgc agggcgtaac ggctgccgag gatttcaaat cccgggtgtt cgggtttcca
    16381 aaggccggac attagaaccg cgccgaccac gcccgaaagc aggaggaagt tccatttgcc
    16441 gaagatggcg attttttcgg gtttttcctg ttgtgccggc gtatcttgtg caatgctttc
    16501 ctgtttgaag aaacggttgt cgatgaaata gaaggcggtc aacaggacag cggtgctgat
    16561 caggacgggg gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt tgaggaagcc
    16621 gaggaagagt ggggggtcgc ccaaaggggt cagaccgccg ccgatgtttg caaccaggaa
    16681 aatgaagaag atgacgatgt gcacgcggcg ggtacggttt tggttggctt tcagcagcgg
    16741 acgaatcatc agcattgctg cgccggtcgt tcccatgata gaggcaagtg ccgtaccgac
    16801 ggcaagcagg gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa ccaaaatgcc
    16861 gcctgaaatg gtgtacaggg caagcagcag caggatgaaa gggatgtatt cttcaacgag
    16921 tgcgtgtgcg acggtatgga taccggcgga cgcgccaaaa accaaactga acgggatgag
    16981 gaagagcaat gtccaaaagg cggtaatttt gccgtaatgg tgatgccagg tatgcgaaaa
    17041 aaacaaggga cccaatgcga tagacagcaa aatcagggca aagggcaggc tccacagcag
    17101 gtttaggttt gcgccgtcca aatctgcggc gtaaaccgat gcggggaaaa gcattagtga
    17161 aaacaggggt aggtggcgca tcgtgtttcc tcgattcaag cactgccttg cgcggcgcgt
    17221 gggagtgata caggcaccgt gccgcccgga cataggcgga ctgtgctgcc tgtttgtttt
    17281 tgaaaaaagt tttccgattg attgtaaagt aaataatcag gataaaccag tttcccaaac
    17341 cggaaggcgg cgggaaggcg gattgctgtg cttgggaata tatgttcttt ttgataaata
    17401 attttattta aaacaaataa atatatgtct ttaataaata atctatcgaa aacgaaaaat
    17461 gaatttattt taacatatat ttgcaatgaa acaggtttgc cccccgtttg tttgccctta
    17521 tccctttcag tacggcattc aagattcggg cctgcgccac atccatatgg cgacaaggga
    17581 acaaaaaacc gatgaaaccg ccccgaccca ccagcgttgg ggaaactgcc aaaacattat
    17641 caggcaggat gcggtcatca tactgatggc gaatattttg gctttgcgcg gcactgcgcc
    17701 gttttgttcc cagttatgaa ccatcggacc gaaatagcgg tgccggtgca gccagcggtg
    17761 aaagcgcggg gatgcctttg cccagcaggc ggcggagagc agtacgaacg gcgtggtcgg
    17821 caacagcggc aaaaaaatgc cgatgatacc caacagcagg gaaatgcagc cgcaggcaat
    17881 taaaagataa cgtatcattt tgaaatattt ttcttattgt gcggataagg gcaggatgtg
    17941 ataccgagtt ttgcccagcc ttcatgtccc attttttcca gcagggcgat attgcgttcg
    18001 aatattgccg aagtgtcggg aaaggcttgt gcggctttgg caatgctgtc ttcgcggatg
    18061 aggtgcagcg tcggataggg agaacggttg gtgtagttgc cgatgtcgtc gggttcggta
    18121 ttctgaaagc ggaattgggg atggaacggt gcgatttgga cgatgccttc caagccgttt
    18181 tcgataacgg cggcatcggc aatatcgagc atatcgttga atacgtcgaa atcggggaat
    18241 agggtcgggt gaaccagcag ggtggtttcc agttcggtgg cgggtgtatt gcccagtcgc
    18301 tgcagttctt cgtccaagtc ttccaaaaaa ctgtcaaggt gtttggcttc gctgattgcg
    18361 atgcggacaa ggtttttaac gtggggggct ttggcaaagg gacacaggtt cagaccgatg
    18421 acggcttttt ccaaccattg tccggtgtgt tcggcaacag catctttatt ttcggaagta
    18481 ttgatattca ttattgtcat gtaaatgtgt ttgcagattg cacgtgcggg aaaatcggga
    18541 agggcactat tccttcagca ggtggttgag cggcagggag gtggtgtgtt tgatttcttt
    18601 taaaacaaag ctcgattgcg catcttgtac gccgtggtgg gacaggagcg tatccaaaac
    18661 aaaatgggaa aacgcgttca tatcggtaaa aaacgcctga agcaggtagt cggtttcccc
    18721 tgtcagggcg aagcagctca agacttcagg ccattttcgg accgatgcgg caaagtcttc
    18781 ccgcgcgtct tttgctttgc ggatggaaac gcggataaat gcctgaagcc ccaggttgac
    18841 ggattccgga gacagcagcg cggcatattg gcggacgatg ccggcatctt ccaactgctt
    18901 tagacggcgc aggcacggag aaggcgaaag tgcgacacgt tcggacagtt cgacattggt
    18961 cagcctgccg ttttcctgga gaacctgtaa gattttaata tcggttttgt ctaaagtgag
    19021 ttggggcata tttgcgttcc gttttaagga attcggattg tctgtccgta tgtttgcggc
    19081 aatccgcaca gatggagacc atattaacat ataaaaagtt ataccgtcat ccgggacaaa
    19141 ttttgttttc ggaaaatcat gtgaaaacag aggcggtcgg tttgcatctc ttttaagacg
    19201 gcttgcccaa accgccgatt caagacataa tcgggaaatg tgcaggagag tgttacaccc
    19261 aactacaatg taaccaccga aggcgcagac acccttaaat cgctcaggta tcagggactg
    19321 cacattgaaa caaacaatct ggagagcggc gttggaataa cgtccaccga aggggagaag
    19381 gccgtctgaa ccaccattca gacaaccgcg caaagcagtg agcagactgg tttgccatca
    19441 tgcggatacg gccgaaaatc tcaggttcaa ggacagatag ggtcatccgc gcacaggtgc
    19501 gcgggtggca tctgaacaaa aaatccggag aaacttgaga atgattactc tgaaaaccac
    19561 cccatttcat caagcccatc aagatgcagg cgcgaagccg gtagattttg ccggctggga
    19621 gctgcccatc cattatggtt cgcaaatcgc cgaacacgaa gccgtgcgca ccgacgccgg
    19681 tatgtttgac gtatcccata tgcttgttac cgacgtagca ggcgcaaatg ccaaagcctt
    19741 tttccgcaaa ttgattgcca acgatgtcgc caagctcgct tttgtcggca aagcccttta
    19801 ttccgctttg ctcaacgaca acggcggcgt gattgacgac ttgatcgttt accgcaccaa
    19861 tgaagccgaa acccaatacc gcatcgtgtc caacggcgcg actcgcgaaa aagatacggc
    19921 acaattccac aaagtcggac aagagttcgg tgtttccttc aatccgcgct acgacctcgg
    19981 catgctcgcc gtacaaggcc ctaaagccat tgaaaaactc ctgaccgtca aacccgaatg
    20041 ggcagatgtc gtccataacc tcaaaccgtt ccaaggcgcg gatttgggca acgactggtt
    20101 tgtcgcccgc accggctaca ccggcgaaga cggcgtcgaa gtcatcctgc ccggcaccga
    20161 agccgtcgca ttcttcaaag ccctgcaagc agcaggcgta caaccttgcg gtctcggcgc
    20221 gcgcgacacc ctgcgcatgg aagccggcat gaacctctac ggcaacgata tggacgacga
    20281 taccagcccg ctcgaagcag gcatgggttg gaccgttgat ttgaaagacg aaagccgcga
    20341 ttttgtcggc aaagctgcct tgttggcatt gaaagaaaaa ggcgttgccg tcaaacaggt
    20401 cggtttgctg ctcggcaaag gcggcatcct gcgcgcgcat atggaagtgt tgaccgacaa
    20461 aggcaaaggc gaaaccacca gcggcgtatt ctctccaagt ctgaaacaat ccatcgccat
    20521 cgcccgcgta ccgaaagatt ttgacggcga taccgccaaa gtgctgattc gcggcaagga
    20581 agcggacgtg cgcgtactga agctgccgtt cgtccgcaac ggtcaaaaac agtttgatta
    20641 atatcggttc agacggcatt ttcatttcat atgccgtctg aaagcaggtt ttaattgtcg
    20701 tccgatacgg acgtttgtag aaagcattga acaaggcatc tgtggatatt gattcatgca
    20761 gatgccgtct gaaaataacc ccctatcaat ggagtatcaa accatgagca gcaacatccc
    20821 ggcagaactg aaatacgttg ccagccacga atggctgcgc cttgaagaag acggtatcat
    20881 caccgtcggt attacccacc acgcgcaaga gctgttgggc gacatcgtgt ttgtcgagct
    20941 gcctgaagtc ggcgcaaacc ttgccgccga agaacaagcc ggtgtggttg agtctgtaaa
    21001 agccgcgtcc gacgtgtacg caccgattgc aggtgaagtc gttgccgtca acgaagattt
    21061 gccaagcgct ccggaaactg ccaacagcga tccttacggt gcaggctggt tcttcaaact
    21121 caaaccggca aacgttgccg attacgacgt tctgctgact gccgaacaat acgcgggcga
    21181 agtggattaa accgcccggc tgcccgatgg caaccgccgg acaaacggaa actgcacctt
    21241 cagacggcat ttttgcggtc ggaggtgcag ttttttgtcc gtgttttaag gaagcagtta
    21301 ggctataata acggtctata ttcatcttta ccgatttttt catgcaactt accgctgtcg
    21361 gactcaatca tcaaaccgca cctttaagca tacgggaaaa gctggcgttt gccgcggcct
    21421 gcctgcccga agccgtccgc aatcttgccc gaagcaatgc ggcaacggag gcggtaatcc
    21481 tttctacctg caaccgtacc gagctttact gtgtaggtga ttcggaagaa atcatccgtt
    21541 ggctcgcaga ctatcacagc cttcccatag aagaaatcag cccctacctt tatactttgg
    21601 ggatgcagga gactgtgcgc catgctttcc gcgtcgcctg cggcttggat tcgatggtgt
    21661 tgggcgagcc gcagatttta ggacagatta aggatgcggt cagggttgct caagagcagg
    21721 aaagtatggg taagaaactc aatgccctgt tccaaaaaac cttttctgtt gctaaagagg
    21781 tccgtaccga tactgccgtc ggcgaaaact cggtttccat ggcttccgct tccgtcaagt
    21841 tggcagagca gattttcccc gacatcggcg atttgaatgt cttgtttatc ggtgcgggtg
    21901 agatgattga gctggttgcc acttattttg ccgccaaaag tccccggctg atgacggttg
    21961 ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa gctcggtgtc aacgccgaac
    22021 cgtgcctgct gtccgatctg cctgccattt tgcatgagta cgacgtggtg gtttcttcaa
    22081 cggcaagcca gttgcccatt gtcggcaaag gtatggtgga gcgcgcattg aaacaaaggc
    22141 agagtatgcc gttgtttatg cttgacttgg ccgtgccgcg agacattgag gcggaagtcg
    22201 gagatttgaa cgatgcctat ctttatacgg tggacgatat ggtcaatatc gtccaaagcg
    22261 gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac gctggtgtcc gagaaggttg
    22321 ccgaatttgt caggcagcag cagggcaggc agagtgtccc gttaatcagg gcattgaggg
    22381 atgagggaga gaaagcgcgc aaacaggtct tggaaaatgc gatgaaacag cttgccaaag
    22441 gcgcaacggc agaagaggtt ttggaaaggc tgtcgatcca actgaccaac aagctgctgc
    22501 attcgccgac ccaaaccttg aataaggcgg gggaagaaga taaagatttg gttcacgccg
    22561 tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg aaaaggatgc cgtctgaagg
    22621 ggtttcagac ggcatttttt gtgcaggccg gcaacatcgt gaaatcccac attatatcga
    22681 tgtaatcaca aagtatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    22741 gtacagatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat
    22801 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
    22861 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
    22921 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacagtgt tttttaccgc
    22981 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
    23041 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggcaa
    23101 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
    23161 cgatgcggtc aatacgcgcg gttattcgaa caaaccgatc gatacgctga tggctttggc
    23221 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
    23281 cggtatcccg catttgcccg cgcccatgcg ggcgatacgc tcaaactggc ttccggcgta
    23341 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc tgaccgttct
    23401 gcaaccatcg aaggagacag gagcggacgt acgatagctg tgcatgcgcc ggacgtaacg
    23461 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ctgcaatgga tgccggtatt
    23521 tatctcgaag aaaccgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
    23581 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gcgagaataa aattgtcggc
    23641 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcacccggc
    23701 gcgcaggtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc
    23761 acgcacaaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
    23821 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat aggctatgtg
    23881 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
    23941 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggca
    24001 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
    24061 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
    24121 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
    24181 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
    24241 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
    24301 gtattgcgcc tcttgatgaa cagtctcgca atcagcatcg tcaaatgggc gcaggcgcag
    24361 tttcccgccg ttctgcccgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
    24421 ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ttgctcaaag aagccgaaac
    24481 gcggcagtcg gaatggggca gggcggaaaa cggttctttg aactagtctg tttcagacgg
    24541 catccggatt caaatgccgt ctgaaaacac aaaaggaaaa accatgacca cacatcatgt
    24601 cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcaacc aagtcgattt
    24661 ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg gcaagtccac
    24721 cattatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga tgcttttggg
    24781 cgaacgtacc ggcagcaaag cgggggcgcg gcttcgcagc caaatcggct acctgcccga
    24841 aaccgttgcg ctgcaccctt cgctgaccgg catcgaaacg ctggattttt atgccaaact
    24901 taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca tttcacaggc
    24961 ggcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg ccttggcaca
    25021 agccctgctg ggcgagccca aagtcctgct gtttgacgaa ccgacaaccg gcctcgaccc
    25081 tgcatcaagg caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg gcgcgaccgt
    25141 attgctcagc acccacgccc ttgccgagtt ggacggacac gccgaccgca ttatcgtgga
    25201 ttaaatttaa tccactatat gcgggtatgg cgggtttgag cggacaaatc agcctgaccg
    25261 tccccgtttt gctgaccgct caggttttat gggttatcat tccgcttgtt ttggcagccg
    25321 gaatttttag aaagcgacaa atatgaaaaa aaccctgttg gcaattgttg ccgtttccgc
    25381 cttaagtgcc tgccggcagg cggaagaggg gccgccgcct ttaccccggc agattagcga
    25441 ccgttcggtc ggacactatt gcagtatgaa cctgaccgaa cacaacggcc ccaaagccca
    25501 gattttcttg aacggcaaac ccgatcagcc cgtttggttc tccaccatca agcagatgtt
    25561 cggctatacc aagctgcccg aagagcctaa aggcatccgc gtgatttacg ttaccgatat
    25621 gggtaatgtt accgattgga caaatcccaa tgccgacacg aagtggatag atgcgaaaaa
    25681 agccttttat gtcattgaca gcggttttat cggcggtatg ggtgcggaag acgcactgcc
    25741 gttcggcaac aaagagcagg ctgagaaatt tgcaaaggat aaagacggta aggttgtcag
    25801 tttcgacgat atgcctgata cctatatttt caaataaatc caaacaaaaa tgccgtctga
    25861 acgttgaagc caaggctcag acggcatttt ttgtcatagg ttcaaacggg gcaggtttga
    25921 acaactgccc cattccttat tcctgaccgg gcgtaacggc ggaataaggt tcacatcccg
    25981 ccgtaattcg gcccgcttgc gccttcggga caaatccaag tgatgttttg cgtcgggtct
    26041 ttgatgtcgc aggttttgca gtgcacgcag tttgccgcgt tgatttgcag gcgcggattg
    26101 ccgttttctt caacaatttc gtacacgccg gccggacaat agcgcgtttc gggcgaggcg
    26161 tattctttgt agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
    26221 tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
    26281 acgccgtcgg gtttcggata atcaatcggc ttacacgcgg cggctttttt aagctgctcg
    26341 ttgtctttgc cgtgatgttt caaggtccac ggggctttgc ctctgaaaat catctgatcg
    26401 atgccggtgt agattgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
    26461 gcggcgtaaa gctcttgata cagccagctt tgttcaaaac gttgctgata atccgccgcc
    26521 tctttgccgc tgtcgaaacc ctccacttct tcaaggtttt ccaacaaggg gaacacggct
    26581 tcggcggcga gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg cggcatattg
    26641 aggaaacccg ccgcatcgcc gaccaaaatg ccgcctttga acgagagctt cggcaaactt
    26701 tgcaaaccgc cttcaatcag cgaacgcgcg ccgtaagcaa tgcggcggcc gccttcaaag
    26761 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
    26821 ggattttgat agtccaaacc gaccacgaag ccgacggcga ctttgttgtc gtcgaaatgg
    26881 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcaccacc
    26941 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
    27001 gtttgcggct ggctgttttg gtcgagttgg aaacgttcga tgatttgttt ggaaagcgaa
    27061 ccgcgacaac cttcggcaaa cagggtttgc tgcgcccaaa gctccatgcc gggttggaat
    27121 gaatcggtcg gctcgccgtc tttgccaatg cccatattgc cggttgcaat gcctttgacc
    27181 gaaccgtctt cgtgatacag cacttcggcg gcggcaaagc ccggatagat ttccacgccc
    27241 atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
    27301 ccgtgattgt cgaaattcgg ggtaatcggc aggttgaacg cttttttctc ggtcaggaac
    27361 aacactttgt cctgcgttac tgtgcgtgtc agcggtgcgc ctttttcttt ccagtcggaa
    27421 atcaactcat tcagcgcaat cggatcgata actgcgccag ccagcgaatg cgcccccacc
    27481 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgttttgttc ggcaagctgc
    27541 ttgagtttga tggcggcaga caaacccgac gggcctgcgc cgacaatcac gacatcgtat
    27601 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
    27661 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
    27721 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
    27781 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
    27841 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
    27901 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
    27961 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
    28021 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
    28081 agggcatagc ctttcgcctc aaaacccaaa tcaggcatga tgcgccgcat atcctcaaac
    28141 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
    28201 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
    28261 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
    28321 agcaacagga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
    28381 ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt tttgttaatc cactataggt
    28441 ttccgtgcgg acgtgttcag attcccgcct tcgctggaat gacggcggag cgatttctac
    28501 ttttccgata aatgaccgta acttaaaatc ccgtcatccc cacgaaagca aaaatcccgc
    28561 ctgtcggatt tcggtttttt tgggcgtttc gggaaactta taaatcgtca ttcccgcgca
    28621 ggcgggaatc cggtttgctc ggtttcggtt tttcgggcgt ttcgggaaac tgatgaatcg
    28681 tcattcccgc gcaggcggga atctagaacg cgggacggcg gcaatattca aaggttgtct
    28741 gaaaattcag aggttctaga ttcccacttt cgtgggaatg acgggatata ggtttcccta
    28801 cggacgtgtt cagattcccg ctttcgcggg aatgacggcg gagcgatttc tgcttttccg
    28861 ataaatgacc gcaacctaaa acccatcctt cccgcaaaaa cagaaaaaca aaaacctaaa
    28921 atcccgtcat ccccacgata acagttgcgt aattgcgtag agtgggcttc agcccaacgt
    28981 tttttctttt tcggtcgttg attggtgggc tgaagcccac ccttgtatat cggaactccc
    29041 gtatcatagc aacaaaccgc ccggccgcca cccgcgccca cccaaggcac acaaccgttg
    29101 cgcagcacag ggagcggcag ggcaacccat cgacacaacc ggacagttgc cggacaacac
    29161 aaccgaatgc aaggcaggtt gatgatgagt acccgatacc attacgcagg tatagtgaat
    29221 taaatctaag gggctgtact agattagccc taaatcccac aggattttaa gctgttgaga
    29281 cggtgtgccg aagttaaatc gaaattcgca ttctttcaag aacagcggga aagatttgcg
    29341 atcgattcca ttgtattttc gcaagacgcg ttttgcctga ttccaaaagt tctcaatgcc
    29401 gttaatgtgg ttctgacggt ctgcacactc cttggaatgg ttgatgcggt aatggataaa
    29461 accgctcacg tccaacttgt cgtagctgct cagactatcg gtataaacaa tactatccgg
    29521 catgattttc tttttgatga cagggagtaa cgtttcagac ttggcattat ccaccacaac
    29581 ggtatagacc cgtccgttgc gtttcagaat gccgaagaca accacttttc ctgccgcacc
    29641 gcgaccacgt ctgcctttac gccgtccgcc gaaatcgctt tcgtcctgct cgacagggcc
    29701 ctcaaaaacc tcatcggcag ccaagaccaa atgatggttg ataaccgtgc ggattttacg
    29761 gtagaacagt gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt
    29821 aacttcgagt acaaaaaaac ggagcagttc tttctgtact tttttcttta atttgcagtg
    29881 cgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc caaaaatata
    29941 ccaaaaacag caaaacaaat tgtaaggata cgtataggct ttgtaaaggt aaattgtgaa
    30001 aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct gatgccctgt
    30061 ccttcccgta tatcttgtgt gttgtcaaag tgcaggctgc tttgaaattg gcattacagt
    30121 cgatgaacca ccattttgct ttatttcagc gggcttgaga tgtatataag aatattgttt
    30181 tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa agcgtagagt
    30241 gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct tgggtataga
    30301 gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt ctcttctatc
    30361 ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc taagtagtaa
    30421 aagttagtaa attattttta a
//
LOCUS       DAWFKN010000017        29438 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFKN010000017 DAWFKN010000000
VERSION     DAWFKN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.017"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..276
                     /locus_tag="WGR46_000759"
     CDS             <1..276
                     /locus_tag="WGR46_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855740.1"
                     /translation="QKANEQEPFIAAVAETLSRTMWGYVMRMDARFEVTFIVDLGHVA
                     FEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGIAACRVVFGDVL"
     gene            273..554
                     /locus_tag="WGR46_000760"
     CDS             273..554
                     /locus_tag="WGR46_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855741.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            554..769
                     /locus_tag="WGR46_000761"
     CDS             554..769
                     /locus_tag="WGR46_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855742.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            772..1278
                     /locus_tag="WGR46_000762"
     CDS             772..1278
                     /locus_tag="WGR46_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0855743.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1275..1469)
                     /locus_tag="WGR46_000763"
     CDS             complement(1275..1469)
                     /locus_tag="WGR46_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855744.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            1481..3100
                     /gene="terL"
                     /locus_tag="WGR46_000764"
     CDS             1481..3100
                     /gene="terL"
                     /locus_tag="WGR46_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0855745.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3179..4738
                     /locus_tag="WGR46_000765"
     CDS             3179..4738
                     /locus_tag="WGR46_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0855746.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLRAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            4731..6077
                     /locus_tag="WGR46_000766"
     CDS             4731..6077
                     /locus_tag="WGR46_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ0855747.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALALPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6181..6678
                     /locus_tag="WGR46_000767"
     CDS             6181..6678
                     /locus_tag="WGR46_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0855748.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            6887..7951
                     /locus_tag="WGR46_000768"
     CDS             6887..7951
                     /locus_tag="WGR46_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0855749.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            7973..8875
                     /locus_tag="WGR46_000769"
     CDS             7973..8875
                     /locus_tag="WGR46_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0855750.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            8913..9386
                     /locus_tag="WGR46_000770"
     CDS             8913..9386
                     /locus_tag="WGR46_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855751.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9383..9808
                     /locus_tag="WGR46_000771"
     CDS             9383..9808
                     /locus_tag="WGR46_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0855752.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            9792..10460
                     /locus_tag="WGR46_000772"
     CDS             9792..10460
                     /locus_tag="WGR46_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0855753.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10457..10645
                     /locus_tag="WGR46_000773"
     CDS             10457..10645
                     /locus_tag="WGR46_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0855754.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            10645..12072
                     /locus_tag="WGR46_000774"
     CDS             10645..12072
                     /locus_tag="WGR46_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0855755.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELAAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12086..12460
                     /locus_tag="WGR46_000775"
     CDS             12086..12460
                     /locus_tag="WGR46_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0855756.1"
                     /translation="MSTEYVGSVILYLGANEVEVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            12636..13022
                     /locus_tag="WGR46_000776"
     CDS             12636..13022
                     /locus_tag="WGR46_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0855757.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13009..13473
                     /locus_tag="WGR46_000777"
     CDS             13009..13473
                     /locus_tag="WGR46_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ0855758.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            13655..13834
                     /locus_tag="WGR46_000778"
     CDS             13655..13834
                     /locus_tag="WGR46_000778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855759.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            13963..14352
                     /locus_tag="WGR46_000779"
     CDS             13963..14352
                     /locus_tag="WGR46_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855760.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPTVTDTPS"
     gene            14310..14558
                     /locus_tag="WGR46_000780"
     CDS             14310..14558
                     /locus_tag="WGR46_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855761.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            14555..16604
                     /locus_tag="WGR46_000781"
                     /pseudo
     CDS             14555..16604
                     /locus_tag="WGR46_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            16604..17971
                     /locus_tag="WGR46_000782"
     CDS             16604..17971
                     /locus_tag="WGR46_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0855762.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            17955..19094
                     /locus_tag="WGR46_000783"
     CDS             17955..19094
                     /locus_tag="WGR46_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0855763.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19095..19724
                     /locus_tag="WGR46_000784"
     CDS             19095..19724
                     /locus_tag="WGR46_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ0855764.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            19785..20138
                     /locus_tag="WGR46_000785"
     CDS             19785..20138
                     /locus_tag="WGR46_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ0855765.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20135..20620
                     /locus_tag="WGR46_000786"
     CDS             20135..20620
                     /locus_tag="WGR46_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0855766.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            20623..21198
                     /locus_tag="WGR46_000787"
     CDS             20623..21198
                     /locus_tag="WGR46_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0855767.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21195..21761
                     /locus_tag="WGR46_000788"
     CDS             21195..21761
                     /locus_tag="WGR46_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0855768.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            21764..22999
                     /locus_tag="WGR46_000789"
     CDS             21764..22999
                     /locus_tag="WGR46_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855769.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVV"
     gene            23162..24046
                     /locus_tag="WGR46_000790"
     CDS             23162..24046
                     /locus_tag="WGR46_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ0855770.1"
                     /translation="MNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIGAN
                     AMNKALTAGYSTIIGADAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLASSE
                     KCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAVNI
                     RSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREWWA
                     NPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKAIQ
                     EMAARIETLETEMKESKK"
     gene            24043..24666
                     /locus_tag="WGR46_000791"
     CDS             24043..24666
                     /locus_tag="WGR46_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855771.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            24667..25155
                     /locus_tag="WGR46_000792"
     CDS             24667..25155
                     /locus_tag="WGR46_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855772.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            25503..26342
                     /locus_tag="WGR46_000793"
     CDS             25503..26342
                     /locus_tag="WGR46_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0855773.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            26602..27135
                     /locus_tag="WGR46_000794"
     CDS             26602..27135
                     /locus_tag="WGR46_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0855774.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27198..28382)
                     /locus_tag="WGR46_000795"
     CDS             complement(27198..28382)
                     /locus_tag="WGR46_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0855775.1"
                     /translation="MTEPTSRRRFLKTCTAAIGAGLLQACGTSATSVPPLPSFHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(28422..28826)
                     /locus_tag="WGR46_000796"
     CDS             complement(28422..28826)
                     /locus_tag="WGR46_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0855776.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7504 a         7661 c         8232 g         6041 t
ORIGIN      
        1 caaaaggcca acgagcaaga gccgttcatc gcggcggtgg ccgaaacttt aagccgcacg
       61 atgtggggct atgtgatgcg gatggatgcg cggtttgaag taacgtttat cgtagatttg
      121 ggccatgtgg ccttcgagca tcaattcact gcggtcaaac aggcgctgaa ggaaaaattc
      181 gaggttgaag ccgatgggga tgcgctgact gtggaatccg accgcctgcc ctatggtatt
      241 gcggcctgcc gggtggtgtt cggagatgtg ttatgagcgg agatacaccg attaccgtgg
      301 aatatgtgtt cggcacgttg gtgtcgtttc tgattgcttt attgtggtat tgggtaaaaa
      361 gcatttcaga cggcctgaaa gaagcccgca aagatcgtga tgaactgctc ggcaggttgc
      421 acagcgtaga aacatcttat cagacgaaag cggaagcaaa agacaacagg aacgaaatct
      481 taaacttgtt gcgtgaaatc aaggctgatt tgaaagaagt cggccagaaa atcgaacgga
      541 aggcagacaa ataatgcaaa accaagaccc tattttaaga gcgttggcag aaatcaacgg
      601 caagcaggac aaattgttac aaaatcagga acgcatggat gcggaaatca agaaaatcca
      661 tgccgattgc cgccgcacgt cggcaaccac cggcgcggcc gcgggcgcga tttcgggcgg
      721 tattgtggct acgggcatcg catttgcccg cgccaagttg gggctgtaac gatggcgcat
      781 ccgaaggcga cccgcgacaa gctgcgggca ctctactgca acggcgagca gagccttgag
      841 acggcggcgg ctttatgcgg cgtatcgctc ggcaccgccc gccgctggcg cgatgaggcc
      901 aaggcgcagg gcgacgactg gaacaagctg cgcgcggccc atacgctggc cggcggcagc
      961 atagacgaaa tcgcgcgggc gatgatgacg tcgtttctgg tgcagtatca ggcgacgatg
     1021 acgatgttgc aggatgcgga agtggaagat ttgccgccga gcaaacgggt gcagctgctg
     1081 gcgagtttgg ccgatgcgtt taccaaaacg gtggcggcga acaagcgggt gttgccggaa
     1141 acctcgcaac tggcgacggc tttggagttg ttgcagtttt tgatggtatt cgtgcaagaa
     1201 aaacacccca aacatttggc tgcctttgtg gaagtgctgg agccgttcgg ggcggaggtg
     1261 gagaggaagt ttggttagtt taatcgtgaa gtaaacgtgt catcagaatg tttaccggca
     1321 acaccaattg caaccaatag atctagatga tcggctaatt ttttcagtga aattttaaag
     1381 ccttgctcct ccaataaatc ttgtatttca gacggcgtgt atttgcccgc atctaacagt
     1441 ttggatattt ctaaatcaat cgatttcatt ttttgccctt atgaaaaata aagatttcct
     1501 caaatccctg tccgccctcg ccgccaatct gcgccaagtc atcgaggccg aagtagacgg
     1561 tttcgatgct tcgccgacgg cggtggccga gcggcgggcg aaggtatttg acccggtagg
     1621 cggttacgag tatttcgtca acacctactt cccgcattat atccgttcgc ctgaaaaatc
     1681 cgacctgcac cgcttcttat tcaggcgttt accggaaatc gtcgaatccc cggaaggcga
     1741 gaacgaagcg gttggcgcgc cgcgcggcga gggtaagtcg acgcaggtga cgcagttgtt
     1801 tactttgtgg tgcattgtaa cgggacgcaa gcattactgc gtgattgtga tggacagcat
     1861 agaccaggca tacccgatgc ttgaggccat caaagcggaa ttggagttta atccccgcct
     1921 gaaaaccgat tttgcggatg tctgcggcca aggccgcgtg tggcaggcag gtaccatcgt
     1981 aacggccaac gacatcaagg tgcaggtagc gggcagcggc aaaaagctgc gcggattgcg
     2041 ccacggccct taccgccccg acctgacgat tttggacgat attgaaaacg acgaacaagt
     2101 ccgaaatccc gaacagcgcg acaagctcaa tgcgtggctg acgaaaaccg tgctgccgct
     2161 cggtggtgtg gggcaaaaat acgatgtgat ttatatcggc acgattttgc attacgacag
     2221 tgtgttgaac cgaacgctca ataatccgtt ttggaaaggc atcaagttta aagccatgtt
     2281 gaaatggccc gaccgcatgg atttgtggga cagatgggaa gagttgtacc gcaatgaagg
     2341 tgagttggtg gccgatgcgt tctatcaaac gaacaaagcg gaaatggaac gcggggcgga
     2401 aacttcttgg gcagcgcgcg gcgtgctggc tttgatgaaa atccgcgccc gcgacggaca
     2461 cgcggctttc gattcggaat atcagaatga tccggtgagc ggtgaagatg caccgtttgc
     2521 ccaagccatg cagttttggg cggaactgcc cgccgatttg gtgtatttcg gcgcgctcga
     2581 cccttcgctc ggcaaagcgg gggccagccg cgacccgagt gcgattatcg tgggcgggta
     2641 tcaaagaagc agcggcaagc tgtttatcgt ggaagcgcaa attaagaagc gtttgcccga
     2701 tttgattatt gaagacgtta tccgcttgca tgcgcaatat aagtgcaagc tgtggtttgt
     2761 ggaaacggtg cagtttcagg agttcctgaa agacgagctg gtaaaacgaa gtgcggcaaa
     2821 aggcattccc gtgccggcgc gggcggtcaa accgattgcc gacaagctgc tgcggattga
     2881 gactttgcag ccgcacatgg ccaacgggtt gattttactt catgccaatc agcaaacctt
     2941 aatccaacag tttcgccatt ttcccaaggc agaccatgac gacggccccg atgcggtgca
     3001 tatgttgtgg tcgggggcaa cggccaacag tgcgccggtg gaatatatgg cggtgacgaa
     3061 acatgggacg gacggcggtt tcggcagcgg cgcatggtga aactgacacc gggcagggct
     3121 tacgattaat gcaggcagca gagaattggg gcaagtttaa cttgcccttt ttttatttat
     3181 gaaaaacatt ttcagcagtg cattgagcaa aattctgccg ggccgctata caccgaaatc
     3241 taccccgcaa acggcggaaa ttacccaaaa cacccagact cacgaacacc cgagcaaagg
     3301 tttgacaccg cagaagctgc acggcatttt ggaagctgcc gagcgcggcg atatgaaggc
     3361 gcagtcggaa ctctttgccg acatcgagga gaaagacggc catatctttt ccgagatgag
     3421 caagcgcaag cgggcggtca tcgggctgga ttggcgcgtg atgccgccgc cgaacagcac
     3481 cgacgccgaa cggcggctgg ccgaagaagt caagggatgg attgagcgtc tgcccgattt
     3541 cgaagacatg atgttcgacc ttttagatgc ggtcgggcac ggctttgcct gtgtggaaat
     3601 cgaatggcag caaataggcg gcctgtggct gccgaaaaac tttatccacc gtccgcaagg
     3661 ctggtttaag gtggacggtg ccgataatgt gcggttggcc aaacaggata atccggatgg
     3721 ggaagagctg tgggcgttcg gctggctggt acacaaacac cgcagccgtt cgggtttgct
     3781 ggtacgcggc gggctgatgc gcacgctggt ttggccgtat ctgtttaaga attattcggt
     3841 gcgcgatttg gccgagtttc tggaaatcta cggcctgccg acgcgtttgg gcaaatatgc
     3901 ggtgggggcg gacgaaaccg ataaaaccac gctgctgcgg gcggtaaagg aaatcggaca
     3961 caacgccgcg ggcattatcc ccgaaaccat gaatatcgaa ttgcttaatg ccgccaacgg
     4021 cagcagcgag ccgtttatgg cgatgatcga ctgggcggat aaaacatcgt cgaaagcgat
     4081 tctgggcggt acgcttacca gcatggccga cggtaaaacc agtaccaacg cgctgggtca
     4141 ggtgcataac gaggtgcgcc atgatttgtt ggtgtcggat gccaagcagc ttgccggtac
     4201 gataacgcag caactgattc tgcccctgct gcggctgaat aaaggcaacg tggatgaaac
     4261 ctgcctgccg cgtttccagt ttgatacgca gctacccgaa gatatggcgg tttacgccga
     4321 atctttgcct aggctggtag agatgggcat gaagattccg ctggcgtggg cgcaggaaaa
     4381 actggcgatt ccgctggctt cggacgacga gccggtattg gctttgcaga cggctgaaag
     4441 cgaaggtgtc aaagttgcgc cgttaagtta ccgccgcgtg gccttgagca ggcagggcga
     4501 aatcttggat atggggcagg cggccatcga taacgcaggt ttgggcaaaa tcgccctgcc
     4561 cgagcatatt gagccgtttt tgcgcgggtt gggtcaggct ttggccgagg gcgacagcta
     4621 tgaagaggtg caggagcggc tgttgcgtgc ttatccgcat ttagacagcg ccgaatttca
     4681 gacggccttg gcgcgggtga ttttcatctc cgacctgtgg gggcggctca atggctgatt
     4741 tgggttttgc cttcggtttg gagccggaag cggcggtcaa atattttgaa ggtttgggct
     4801 accatatccc gcccgactgg gatgtgaaat ggaatgaggc gcagaccaag gcgcggacga
     4861 ttgcaggcat acacaggcag gatattgtcg gcgagtttca cgcggcaatg tatgaggcgg
     4921 cgaaatcggg caaatcgttt gaggcttggc gcgatgaagt gcaaggccgt ctgaaagcgc
     4981 atgactggca tctgctcaaa gacggcgata ttgtggacgg agacaccggc gaagtcctcg
     5041 gacgcggtat taccaaacac cgcatggaaa cgattttccg cactcagatg caatcggctt
     5101 acatggccgg tcattggcag gcgtttgaag aaggtcggga tgatgcgccg tggctgcaat
     5161 attcggccat tttggacagc cgcacccgcc aaagccatgc ggcggcgcat ggtgcggtgt
     5221 atcacatcga cgacccgttt tgggattact tctacccgcc caacggcttc aattgccgct
     5281 gtaccgtgcg ggcgctttca gacagtgatt tgaaacggcg caatctgctg ccgcaaaaag
     5341 cgcagcttga agatacggaa gtggtggtca accgcaaggg cgatacccgc cccgccaaag
     5401 cggtaaagct gccggacggc cgccgctttt ataccgatgc gggctttcag cacaatgtcg
     5461 gtaaaagcca tttggccaac ttggggcagt tgcagatgca gcgtgcagtg gaactgccgc
     5521 cgaaactggc gagcgtatcg attcagacgg ccttaaaaca gcctgatttg atgcgggcgg
     5581 tatcgcaaca ggcggcgcaa atggtacggc gggtggatgc ggaaaaagta gcgcgcggga
     5641 aaacgctgta tgtgggcgca ttggccctgc ccgtgttgga cgcattggcg gcaaggaaaa
     5701 tttacccgca atccgccgtg attgccataa gcgacgagcg ggtattgcac gctttgcgcg
     5761 acaagaaggt caagccgctg ccggtatcgt tttgggagaa gatacccgaa ctactgcaag
     5821 agccggagca gattttattg ggcaaggcgg gacgaaatga cgatgccaaa cagtttttgg
     5881 catttgtgta tccgctgtcg gcaggcaagg ggaagctggt ggtaacgctg gactatgatg
     5941 tgaaaacccg acatccgttg acaggtaaga aagaacatct gaccttgaat atggtcaaca
     6001 ccggcatgat tgcagaaacg gataaacagg tggatagctt actgtacggg tatgaaacaa
     6061 tatggaaaaa gccataaaac tctttgcctg attcgaacag gataatacgc gacacggtaa
     6121 cgtggccgta acctttccag taggaaaccc gagttttatg gctgttggaa ccaatatacc
     6181 atgattgaga taaaaatcaa cacagacacc ctgcaaaaca gcttaaacgc tgcggcgcgg
     6241 catacctccc acaccaagcc tttgatgacg cggcttgccc gcatcatgcg caacgccgta
     6301 ctggagaatt tcgccgccgg aggccgtccc gcttgggcgc cgcgcaaata tccgactgcg
     6361 cgtgaaggtt cggggctgtt gcaagccagc ggccgcctgc gcaattcgat tacgccgagc
     6421 agtaccaacg atacggcggt ggtcggcacc aatgtggaat atgcggctat ccataacttc
     6481 ggtgggaaaa cctcgccaca cttgattaga ccgaaaaaag gcaaagcgtt gaaattcggc
     6541 ggccgctttg ccaagcaggt taaccacccc ggcagcaata ttcccgcacg cccgtttatg
     6601 accctgcaac cggaggacga aaaggcccta tccgatgcgg tggcggaata tctggcacag
     6661 gccattcagg ggcggtaatc atgcctgcct gaaaaatgcc gaaaaacagc ccgaaaacgc
     6721 cctaacccat acttacccct acccatcgcc tgaaaatcaa tcctgcgcgc gtttgaacac
     6781 cttttgaaca ctatcccacg cgtatctctg caagtacatt tcccttccct agaaattcat
     6841 ctgtctgaca taggccaagc tttgacgagg gcgcggctgc cgcacaatgc gggctatgga
     6901 caccaaaacc cttcttgccg ccttatccgc cgcccatgtc ggcggttcgg acggcttaat
     6961 caaaatcgta cccaaaggcc aattcgcacc ggttgacggc cgcaccgata cgggtgtgcc
     7021 gcactggacg atgtctgccg atttggcaca gcaaatcatt gccgcctttg acgccgcgca
     7081 aaccgacctt gtggtcgact acgaacacgc cacgctgaaa gccgccgaaa cggggcagca
     7141 aaaccctgcc gccggctgga tcagcaaata tgtatgggat gacgagcgcg gcctgatggg
     7201 cgaagtgaaa tggacgcaac gcgccaaaga catgatagac agcggcgaat accgctatct
     7261 gtcgccggta ctcgaatacg acacgctggg caatgtacgc gggctgcaca gtgtggcgtt
     7321 gaccaattcg cccgcactgg acggcatggc tctggctgca ttgagccgcc aaaactctat
     7381 caaccccaaa caggaaacaa gtatgaacaa ggaagcttta atcaagctct tgggcttggc
     7441 ggcggatgcc gacgacaaag ccatcgaagc ggctttggcc gaagcacagg aaaagctggg
     7501 cggtaaaacg cttgccgaag cactggccgc acccaaagaa gaaccgcaag gtagcgaagg
     7561 cgataaaggc gatgccggca aacccgaaga caagccgcaa ggcggcaatg ccgacgacgg
     7621 cgaggtagcc gaactcaaag cgcaagtggc cgcgttgagc aaaaaagtga ttgcaatgga
     7681 agtgggcggt acttcagacg gcctgatccg tgccgcgctt tcagacggcc gcctgttgcc
     7741 gcatcaagaa gcatcggcgc gccaactggc cgccaaagac cctgaagcgt ttaaagccct
     7801 gattgacggc agcttggcac tggccgcttt gagcaaaacg cagaccggcg gcaaaggcgg
     7861 cgcagacggt acgtccgcgc tgaccgccga agaagcagcc gtcgccgcgc aattgggcat
     7921 ctctgccgaa gattatgcga aggccaagta atccgataaa ggaaaagaca acatgattat
     7981 cacaccagac accttaaaag ccctgttcac cggctttaaa aagaatttcc aagacggcct
     8041 gaaaatggcg gaaagccaat acaaggaaat cgccaccgtc attccgtctt ccactgcgtc
     8101 caatacttac ggctggctcg gccaatggcc cgctttccgc gaatgggtgg gcgaccgcgt
     8161 attccaagat atgaaggcgc acggctatgc catcaccaac aagcattttg aaagctcggt
     8221 caaggtcaac cgcaacgaca tcgaagacga caacgtcggc atttacgcgc cgatgatgac
     8281 cgagatgggc cgtgcttcgg cggtgcatcc cgacgaattg gtgtttgcct tgctgaaaaa
     8341 cgcacacgcc acgttgtgtt acgacggtca gaacttcttt gataccgacc atcccgtgta
     8401 tgaaaaagtg gacggcaccg gtcaagccag taccgtatcc aatattttcg ccggcagcga
     8461 agcggcctgg tatctgctgg acactacccg cgccctgaaa ccgttgattt accaagagcg
     8521 taaagcgaag cagtttaccg ccatgaccgc cgataccgac gaaggcgtat tcatgcgcaa
     8581 cgaataccgc tacggcgtgg acggccgttg caacgtgggc ttgggcttct ggcagatggc
     8641 ggcgaaatcg caagagaaac tggacgctgc cggtttcgag aaagcctaca acgcgatggt
     8701 gagcctgaaa ggcgacggcg gcagaccgct ggccatccgc ccgaatgtgc tgctggtacc
     8761 gcctgctttg gaaaatgcgg ccaaagagct ggtggaaggc gaccgcctgg ccaacggcgc
     8821 gtacaacccg aacaaaggca aggcgaaagt catcgtatcg ccttggttgc tgtaacgaac
     8881 aggcgggcac cgcccgccgg aagggataga aaatggcaaa agaaaaaaac ggacaggaag
     8941 ttggcgcgac cgtcgatgtc caaccggaag aggtaaccgt aaccgctgcc ttacaggccg
     9001 agattgaagc cttgagagcc gaattagaca aagccaatgc cgaaattcag gtagcgcagg
     9061 cagagttggc cgccgcgcaa gagcgcaatg ccaaattgga aagactgctt gaagccggcc
     9121 ttgcacccgg cggcatttca gacggcgaaa ctttgacacc tgaagcggaa gccgcctttt
     9181 acaccggcag cggagcaccc gccgcagatg ccgaagtggt ggcgattaag agcaaacacg
     9241 gccatgcgtt tttccgcgcc ggctaccatg tacagccgaa ctggacgttt gtgcgccgtg
     9301 ccgacttcga acccgccgac tttgagcgcc tgattggcga ccgtatggtt gaagcgcgcg
     9361 aagccctgcc gacggacgca tcatgagcta cgccgccgtt gccgatttgg tggcgcgttt
     9421 cggcgaagcc accatcaccg gcctgaccga cttgtcccgc aagggcgcgg tggacgaaac
     9481 cgtcgcacag caggctttgg atgatgccgc cgccgaaatc gacggctatc tgatgaaccg
     9541 ctataccctg cctctgccca agccgctgcg catcttaacc gtgtattgct gcgatattgc
     9601 ggtgtaccgc ctgtgtaccg gcaaacggca gcttaccgaa gacattgtgc accgctatga
     9661 agcggcagtg aaattcctgc aactggtggc ggcggggaaa gtcggcttgg gcgttaccga
     9721 acccgcaggc gaaaagcccg ccgtacaagg caacggcgtg atgtttacca cacaggaaaa
     9781 ggtgttcggt cgtgatagcg tctattgaac aagccatcag gcagcggctt tcagacggcc
     9841 tcggccaaat ggtaacgggt gtctttactt acggcggcga gttcgacggc gaaggtttgg
     9901 cgcaggtggt caaccagttc cccgccgtgt gggtcatgtt tgccggcatc aaagacacca
     9961 tccgccacga tacgcgcggc agccgtttta aagcgattgg ccaattcacg gtattggtgg
    10021 gagaccgtgc cagcggcagc gaagcagaca gccgtttcgg cggcctgcac cgccatgatg
    10081 tcggcaccta ccgcctgatg cagacctgcc gccacctgct gaccaatcaa tcgctgggct
    10141 tacagataga ccgtctgcaa ccgggggcgg ccaaaagcct gttcagccgc caaatggagc
    10201 aagacgccgt tagtgtgttt gcgctggaat ttgaaacgta ttggtttgaa gacgcgctgc
    10261 aagacggcga ttggccgcgg cctgcggttt cagacggcca acaggcgaaa gtatatgccg
    10321 acgtatccga ataccaaggc cgcaccgaac ccgaacatcc cgactttaaa ggcgtgaacc
    10381 ttgaattgcg tatcccgccg aaaacccccg accaacccgc cgatatggcg gccaccgttg
    10441 aaaccaaggt gaaaccatga acgaaaccat taaagtacgc gccgccgacg gcctgcaagt
    10501 gccgctggcc ggtaaaccac atgaatacat taccgaccaa gaaaccgtta ccgtacccga
    10561 tgccgcctat taccgccgct gtatcgaata cggcgacctt gtggtcgtaa ccgaagacac
    10621 ccaacccgcg aaaggcagca aataatggca tccgcaaaca tcaatttcga aaaaatcccc
    10681 gccagcaccc gcaagccggg cgtgtacgcc gagtggaaca ccaagctggc cgtgcgcaac
    10741 ctgcccacca acaaacagcg cgtgttgatt gtggcgcagc acaacaatcc cgctttgggt
    10801 gagcttaccg agctggaaaa cgtgttttcc gccgccgatg cggccgccaa atacggcgca
    10861 ggcagcatgg cgcacctaat ggttaccgcc gcgattaaag cctatgccta tgccgattta
    10921 agcctgatta ccgtagccga caacaaggcc ggagtggccg ccggcggtaa aatcacctta
    10981 agcggtactg ccaataccca aggcgtgttg cgtgtgagca ttgccaatgc tgacacttta
    11041 accatcggca tcggcgccga agacaccgca gccaccgttg ccgccgccgt caaagccgcc
    11101 atcgatgccg tgcccgattt gccggttacc gccaccgttg ccgaagcagt ggtgacgctt
    11161 accgccaaaa acaaaggtac ggccggcaac gccatccgca tcaaaaccag caataccgcc
    11221 gaaggcatta ccgccgccgt taccgccatg acgggcggcg atgccaaccc cgatattgcc
    11281 gccgcgctca atgccgtagt cgccgaaggc caccacatca tcgcctgcgg tatcaacgac
    11341 gaaaccaacc tgctgaaact gcgtgcccat ttggataccg tagccagccc gatggaaaaa
    11401 cgctgggcaa tatgcgtata cggccaaacc ggtacgctgg cgcaagccac caccttggca
    11461 ggccgtctga accacggcca tatcgtcagc gcgtggtatc gcggcacccc cagcctgcct
    11521 tgcgagctgg ccgccgcctt tgccgctgtg atggcgagcg aagaagaccc cgcccgcccg
    11581 ttgaacacgc tggcattaaa cagcatcggc gtgtgcgaaa gcaaagacaa aaccatgcgc
    11641 accgagcagg aaaacgccct ttataacggt gttaccccga ttgaaaccag tccggccggt
    11701 acgcaggcgc aaatcgtgcg cgccatcacc acctacacca aaaccgccaa cggcacggca
    11761 gacgaaagcc tgctggacgt aaccaccgtg cgcaccctga tttatgtaag ccgcgcctgc
    11821 gtcgaccgca tcgccctaag attcccgcgc gacaaattga gcgaccgcac cccgccgcgc
    11881 gtgcgcagcg aactgattga cgtattgatg tgctgcgaag agctggaaat tttggagcgc
    11941 gtggaagaaa acctgccgaa actgattgtg gaacgcgacc tgcaaaacac cggcatgttg
    12001 aactgccgca ttcccagcga tgtcgtcaac ggcctgcatg tagtaggtat ggttgtagat
    12061 ttatatttgt aagaaaggct gaaacatgag taccgaatac gttggcagcg tcatcctcta
    12121 tctgggcgcg aacgaagtcg aagtaaccaa aatcgacgtg aaagacaaca ccggcaaaaa
    12181 gcccgtaaaa accatgaacc gcacccgccg cgtcaaaggc tttacccgcg gcgtcggcca
    12241 atacgacatt acctttaccg ccgtggtgcc gaccgacggc acggcggtgg actgggcaaa
    12301 gatcgacgac gccaaaatca gcctagttcc cgacgtggaa ggcgcgcgcc cgacatcgta
    12361 cctcggcttc tgcgccaccg aagcgggtga cagctatacc gtagataatg agctggtggt
    12421 ggatgtgacc ggttttgcga ttcggaaggt gttggagtaa agattttctt gttaacagaa
    12481 gccgctgaag cggcttctgt tgtttttatt acactgtttt taataaaatt caatttttca
    12541 agtaaaatac ttgaatttta aaaaagcctt tgatattatt tagccacgtt gaatgtggca
    12601 gtaatgctgc aaacacatga aaaggagctt gaacaatggg actgactaaa ttcggcgcag
    12661 ccgttcgtga ggctcgtcgt caaactaaac aaactctgca aacgatggct gctacattag
    12721 gtacttctcc tgcttttttg agtgctattg aaactggtcg tagtaaagtg ccaatggatt
    12781 ttgttaaaaa ggttgaagat ttttttgaaa acttaggcca accaatagac ggtttaaagc
    12841 aaaaagcgat ggtttctaat gagaatgtat cgttaagcgg cttaagcctg caacaacaaa
    12901 tgctggtggc tggttttgcc agttcagact tcagcaaaga gcaattggac aaatttgctg
    12961 aattattaag aaccattcat cataaagatt cacagaaaga aggggatgat gcagcaaact
    13021 aattattcaa tgcgtggtgt gcgtgtggga atgttaagcg aagaaacgat tcagtttttt
    13081 gcaaacgaag ctgccaaatt tttggtagta gataagcgta ctcgtaaaaa aatggatgca
    13141 tttatggaaa tgctggaaga gtatggcatt gtaattgatg ttgttgctga ttctgaatgg
    13201 ctagtcatta ccaacgcaat gtgccataac ggaacgattc tgatgcctaa tagcctctat
    13261 actcgcattt gcaatggcga ggacgaggct atctttattt tctttcatga attggggcat
    13321 ttgcttttag ggcataaagc catgttgcat tatagtgaag ttcttcccac gaaacaggaa
    13381 gattcagaat ggcaagctga tgagtttgca aaatgtattt taagaaacat gggaattaaa
    13441 tatgtaccag aacagttaag cttacgattt taaaaagaga aatgacttgg aatgttggcg
    13501 catcccaagc cattattgtg gtgtactagc atacttccaa tatgctgtac aagtgtagtt
    13561 ctccatccgc taactgcaaa cagatttcaa agagaaatac ttgaaaggga cttgagtata
    13621 tatttttcag atttgtttgt ccacgaaaag cgaagtgatg gccaccattt ttacaattgg
    13681 aggtgccatt atggctatga ctaaggctcc cgttaccccc gacttggtgg acgatacagg
    13741 tgcatacatt tttagaaagt cttttaccac caaaagtggt aagaaaattg tttctaaaaa
    13801 tgggaagcca tttaaaattc ctgtgaaagt ttaggaatat ataagccccg ctaagcgggg
    13861 ttttttcatt actgacgcgt gtttagtcgc ccatctcatc tccttaagtg acaattaagc
    13921 cttgatttat acaaggttta accaacgttt aagggctttt ttatgtctga attttccccc
    13981 gaactcaccc gcgctatcga agattacgaa ttacgcgttt ctcccgacct gaaaaccgtt
    14041 gccggccgtc tgaaatacgg catcggtgtg attgatggcg aaatgcacca tgatttttcc
    14101 atgcaccttt taaccgtgcg cgaggacatg gcgattgatc cgcaacttga aggccagccg
    14161 cggctggttg ccgcctatgc cgcgtcactg gataaattgg gtggtttaac agccgaatct
    14221 ttgacacctg atttgctgct tgatgagatg gctgctgctg actttgacgc gctgtactgg
    14281 gcgcaagagc tgctgcaaaa aaagcggctt tgcccgcacc ccgcgccgac cgttaccgat
    14341 acgccgtctt aaaactcggc cgatacggca tcacgcccgc ccaaatcgac ggcatgaccc
    14401 aacccgaact ggaaggctgg ttaaaacagg ccgacttaat cgagcgcggc cgtgccgcgc
    14461 cggtagtgat gccgtggttt attcctgcga acgtcaaacc atctgccgcc acgggcggtc
    14521 agacccaaac ctttatcagc aagcgtaaga aaaaatgagc cagcatagtg tagaactggt
    14581 tgctaagttt aaggacaacg ccacgcccgg cctccgccgt ttggcggcgg aagcggaaaa
    14641 aacaggcagc cggcagttgc agaaagccgc agcagtcaag ctgaaacatc aagagatgta
    14701 cagcgctacc gcccgtttgg gcatccgtac tgaacaccaa atccgccgtg aaatccaaca
    14761 aacgcaggcg gcttacaacc gtttggccaa aagcggcatg gcttcgcaac gcgaactggc
    14821 gagggctgca cagataacgc gcagccgtgt acgcgagctg actgccgaaa tcaacggcgg
    14881 tgcaagccgt ttgcaacgta tcggttcggg agtccggaca atcgggcgcg gtgtggccgg
    14941 cgtggcggcg ggtgcggcgg caggagctta tgtattggcg cagccggtca accggacgat
    15001 ggactttgac acatcattgc gtcatgccgc caataccatg tatgcaggca agagcatggc
    15061 cgagaagcgc gcaggtatgg aggaaatcaa gaaatcggtc atggatgccg cctatgtcgg
    15121 cggaacgacg cgtgatgccg ctttggaagc catgaataca atggtggcca gcggagcgat
    15181 gagcgacgaa gccgtgaaaa aactgctgcc gaccgttatg aaaaccgcca ccgccgccaa
    15241 tacggaagga aacgatattg ccaacatcgt aaccaaggcg ttgcaggcgg ggtttaaaga
    15301 ggcggatatt ccgtcgctgc ttgaccgtgc gttgcaatcg ggcgcggacg gcggttttga
    15361 attgaaagac atgtcgcgct ggctgccgca gcagttggct gcaatgaaaa acgccggtat
    15421 gggcgcgacg ctggataatt tttccagcct gctcaatgcc aaccaattgt cgtttatgac
    15481 ggcgggcagt accgacgaag ccggcaacaa cttagtcaac ctgctggcaa aaatcaacag
    15541 tcaggatatt gtgaccaaag ccaaaaaaat taccatcaac ggcaaagaag gtttcgattt
    15601 caccgctagc atgaacaaac ggcaggcagc aggcatgaac tcgcttgatg cgctggtgga
    15661 cattgtcgga gaaatcacgg ccaaggataa gaagtccgcc gctctgatga aacagattaa
    15721 tgccgcgcaa ggcgatgaag ccaagctggc tttgctggaa aaccaaaagg ccttggtaga
    15781 cggcactgcc atcgggaagc tggtttccga ccgtcaggca ttgatggcat tgctggcatt
    15841 ggtcaacaac aaacaggaac tcacccgcct gcaagcaggc cagacaaatg cggcgggtgc
    15901 ggtggacggt gcatacggat tcgtcgccga aggttcgggg tttaaaaaat cccaattctc
    15961 cctcgccaaa tcagaagccg aatacggcgc atttgaaaaa ttcagcgacc gcatcggcgg
    16021 cggccggtac aagctctatg tttgggggcg gcggcctgct ttccaaactg ttcggcggaa
    16081 gcagcagaac cttacaggcg ggccgtctga tgccctcggc ctcctcgcta ggccccgtcg
    16141 cgctcggtgc gacaccgttg gccgtaatgg gcggggcaac ccatttggcg gggcagcgcg
    16201 acaaatatgc ggaatggtca aaaccgtttg ctaaattatc gtcttggctg gaaagtatcc
    16261 tgcctgattt ttcagcgggg gcgaaagccg aatatctgcg caaacgtgaa gaactgggcg
    16321 gcaataatgc cccgctcgat agtcctgttt taaaagagag tatggcgcag cttaaccaat
    16381 cggcacaaac caaccagcag gcaagccagc agtatgtgca ggcggcggcg gagaatcagg
    16441 cggcaacggc gcagattacc ggcgcggcgg cacagatgac ggctgctgcc gcacaaatgc
    16501 aggcggcggc aggcaagccg attccgatta ccgttactgt gcaaaacggc aacattatgg
    16561 cctatgtgaa tcaggcagta gaacgcaaca gtaggaaaaa ttaatggctt ggaaagacac
    16621 tttactggat gcgggcttca agggcgttac ctttgacgtc atcgacgaca cgctgcgcgg
    16681 cacccacgcg ctggcggaac acgaataccc gttcgtacag ggcgcggaca tcgaagatac
    16741 gggtgtatct gcgatggata tggccttgac cgccgtattg tggggtgaag attacgaagg
    16801 ccgcctgcaa aacctgctga acgtattgtg cgaaacgggc gcgggcgaat tgatccaccc
    16861 gatttacggc agcgtgccag attgcgtagt ggcggatttc gaggtcgcgc acaacgaaga
    16921 aaaccccgac tactgcaccg tgcggatgac ctttaagcaa agtgtcaaag ccgcgccgtt
    16981 tttcgaccgc gacttgccga tagccttggc cgacgaagtg gatttccttg ccgacttggc
    17041 cgcatggcag ggcttcgagg tgtttcagac ggccttaaac aaaattcaga aagcgcaaag
    17101 ccgctggaat gcttttcatg ccacggtatt aatggtggtg ggcgtattgt atgggcaggt
    17161 aaacggcatc ttcaccggcg gcctgaacct gctgaacagc ccgcgcgtat tggtggcgga
    17221 gctgaaatct gtgtttggtg cgctggccaa tatgcacaat gtcggtaaaa gcgggctgga
    17281 cggctggcgc gacatggtgg gcggcgtatc taaagcggcc gctacgccgt ggcaggtcag
    17341 ccgcggaacc gaaggcagtg tggcggcggt agatttaatc caacgcgcca agcccgaaga
    17401 tgtggctgcc tttaccgcgc ttaccgccac cgttggcgcg tgtgcgctgg ccgaacaagc
    17461 cgccgatatt ttggccgtac aactgaacga gccgactttg acgcctgtgg aaatctcgcg
    17521 tctgctggcc gatacccgtg ccgccttgca gcgtgctttg gccgcccagc gcatcttggc
    17581 catgatgctg gcggatgaaa ccaaagccga caagctggcc tattgcctgc tgcggctgta
    17641 tcaaacaccg gtagacagtg ccgatgatgt gtaccaacgc atcgaagtgg ccgggctgct
    17701 gccgcaagcc ccttatctgg aaactgccgc cgcgcttacc gaaagtctgc gcaatacggc
    17761 gcataaactg caaaaacagg cgtttacagt catcaatatg aagccgccgc tggtacagaa
    17821 aaccgtcacc tgcgatatcg gcctgcacct gctggccttt gagtggtacg gcgactacag
    17881 ccgctttggc gaactcttgc gcctgaatcc gcaaatccgc cacccgaatt ttattgagaa
    17941 aggcacggta ctgaatgcct acgccagata acaccgttac gctgctgatt aacggtaaaa
    18001 cacacggcca atggacgaat tacgacattg tttccgacct gctcaccccc gccgatgact
    18061 tttcagtcat gctcggccgc cccgtcgatg ccaaacccga tgcggtgcgg gcgggcgaca
    18121 aggtggaagt gcgtgtgggc ggcgatacgg tattgagcgg ccgcatagac cgcgtgcaga
    18181 ccgttaccga aaaaggcggc aaaaccttaa ccatacaagg ccgcgacgat gcgggcgtgc
    18241 tattggactg ctccgccccg ctgtttaacg cgcaagatat ggatttaaac caaatcatcg
    18301 aaaaaatcgt caagcctttg ggtttggcca aaatccgcat tgatgccgcc aaaaccgaca
    18361 aaacccacaa agtgcagatc gagccgggca gccgcgcgtg ggacgccctg ctggaatatg
    18421 ccgaggcaaa cggtttgtgg ccgtggctgg agccggacgg cacgctggta gtcggcggtc
    18481 ccgattacac cgccgcaccg gtggccgaac tggttttacg gaccaacggc caaaacaaca
    18541 acatcaagcg tctggaagta aaccgcgata tggcggcacg gtacagcgaa gttaccgtat
    18601 tggcgcaaag ccacagcggc aaaaacaaca tcaaagccac cgccaaagac gaatccgtca
    18661 aactgcaccg ccccctaatc gtcaccgagc cggacatcga cagtcaggct caagcgcagc
    18721 gcaaggcgaa aaagcggctg gccgacagca ggctggaagg cttaacaatt accgccaccg
    18781 tacaaggcca ccgcactgac gacggtacct tgtggcagcc cggtcagcgc atcaacgtat
    18841 tgagcgaacc ggacggcatc gacgcggtgt atttcctgat ggcgagaacc ttcatcggcg
    18901 gtcgcggcca acccaccgaa accgtgctga cactgaaaga ggacggcgca tgggtattgg
    18961 atgccgaccc gccgaagaaa tcgggcaaaa ccaaaaggcc gtctgaaagc cgcaaagcca
    19021 acggccacgc agccgccaaa ccgaaaaaac gccgccaagc caagcaggcc ggacaggaat
    19081 tgcaggtgat ttaaatggat gcaaaaacca tagacaaccg tatccgacgg gcatttaacg
    19141 gcatccgcca agccttccgc ggcaaaatcg cccgcgtcaa agcagccggc ggcgtgcagc
    19201 aaatccaagt ggaaggtttg gaaggcgaaa ccgtgcagga cttggaacac gccgaaaact
    19261 tcggttttac gtcgaacccg cccgcaggca gcgattgcgt cgtcgtaccg ttgggcggca
    19321 aaaccagcca cggcatcatc gtcaccacca ccaacggcgc ataccgcatt accggccttg
    19381 ccgaaggcga aacggcggtt tacaacgccg acggtgccaa gatggtgtta aagaaaggcc
    19441 ggattatcga aattgactgc gaaacgctga atatcaaagc accgggcggc gtgaatattg
    19501 acgcgcccaa tgtaggctgc accgcgcaga ttaccgccga gggtcaaatc aacggcaacg
    19561 gcggcatggc agtgaaaggc ggcagcggca catcatttac cggcaatgtg cagatggtcg
    19621 gcgatttgga cactaccggc aaactgacca acaacggcaa aaatgtcggc tccgaccaca
    19681 aacacaacgg cgacagcggc ggcacgacat ccgacccgct gtaattcaga cggcctgaca
    19741 tccatcaaac caaactccaa gcgtccttac ctgaaaataa aggtatggac gctttactta
    19801 atccccaaac gggcggctat gtggtcaacc aatccgccca atccatcgaa aacgagttgt
    19861 atatccgctt ggtcacgccc ttgggcagtt actgggcaga ccgcacgctc ggcagccgcc
    19921 tgcacgaatt gcgccgccaa aagcatttaa agcgcatcga agtactggcc aaacagtacg
    19981 ccgagcaagc cttgcagccc gtgattcagt ccaaacgcgc ccaatccatc caagtaaccg
    20041 catccgcccc gcagcacggc tggctgaagc tgcatattga agccgtcgat gccgcgggcg
    20101 atacagtaac cctgaaccac aaggtagccg tgatatgacg caggcactga atttagaaca
    20161 aatccgcgcc aactatctgc gcgacctgca aaaccaaaac cctgccgccc atgtacacgc
    20221 gggcagcgac aaccatgtac gcgccaccgc cattgccgca gtgggcgaag gccaatacca
    20281 gcatcaggag tggattttgc gccaagcctt tgccgatacc gcagacagcg cctacctaga
    20341 aaaacatgcc gctaaatacg gcatctaccg aaaaaccgcc accttcgcgg gcggcaaggt
    20401 gcgtgttcgc ggcgcggtcg gcgcaacggt gccggttggc caacagatca atgtgggcga
    20461 taaggtgtat ttaaccgccg aatccgccgt tatcagtgcg cttggaagtg ccgaaatagc
    20521 cgttatcgcc actgttgcgg gtagtgctca aaatcaaacc gccgaaaccg cggctacgct
    20581 gcaaagcgta cctgcgggga ttgacagttc cgccgtctaa cgatggtcgg cggtacagat
    20641 gccgaaagcg atgagagcct gttggcacga tatgaagaac gtctgcgccg acccgctgcg
    20701 ggcggtaatc aatacgactt ccgcaattgg tgcttggaag taccgggtgt ggttgatgcc
    20761 tttatctacc ctttgcgtcg cggcaacggc tttgtcgatg ccgtcatttt gggtgaaaac
    20821 ggtatcccca gcgcagaaac actggccgcc gtacaagctc atgtcgatgc ggtgcgacct
    20881 gttacccgta aaaacggctt tctagccctt gcgcccagta tccagaccgt gaatgtggcc
    20941 gtcaccatta ccttaagcag cggcacggat accgatacgg ccaccgctgc catcaaatca
    21001 gctgtaaacg cctattttga tgccttaaag cccggagacc ccctaattaa aagccagttg
    21061 gaaaccttaa tcagcgaagt gtacggcgtg cgtgaccgtg tcttaaccac ccctgtgggc
    21121 aatatcaagc ctcaggagag tgccgaagac atttactggc tgcgcccggg cagtattaac
    21181 gtggagtaca ccacgtgatc catcaaacct tactcgccgc catgcgtccg cccgtcagct
    21241 acgacaccgt aggagaaacg gcagaaatca aagccgaagc gggtgtgttt gatattgtgg
    21301 ccgaccatgc ggaaggagtg aaaaatgcgc cgttccctga tgcggaaaac gattacctgt
    21361 accgctggga agagctgctc gccatcaccc cgcccgcagg agccaatacc caacaacgta
    21421 ctgatgccgt gctggccaaa ctcaatgcct tgggcggttt gagtattgcc tactttaccg
    21481 ccatcgccga atcggcaggc tacaccgtca ccatttacga agaagaccaa ttccgcgcag
    21541 gtgaaagctg tgcgggtgat tgtttgaata ccgaagacgc catctggcgt tggtgcgtcg
    21601 acatcgccga cggcaaagcc accgcctata ttttccgagc cggtcaaagc cgtgcgggcg
    21661 accgcatcag tgtgtacacc gacccgatta tcgaaacgat gtttgaagaa ttaaaaccgg
    21721 catggacgta ttgccgcttt gaatatgaag aagaggtata aaaatggact taatccaaac
    21781 cccgaataag caatttgtcg acggcgaccg ccgcacgccc ggtactcccg tacccgcatg
    21841 gtggctgaac cagttacaag gcgagttgta cagcatttta aacgcggttg gcattgagcc
    21901 taacaaagcc gaccatgccc aagtcttatc ggccattaaa acgttggccg ccgatgcttc
    21961 gcaggttgcc agtatcgatg ctctgcgtaa atacagcggc acaggctatg tgaacgtcaa
    22021 cgcctatcac gccaatacaa cagtgggcgg cggcgtgttt gtggcggata aagccgataa
    22081 atctaccgct gataacggct gtaccgttat tgtttctacc gacggcacgc gctggaagcg
    22141 tgtgttttca gggatgctta acctgcatga ttttggatat gtggccagca aaaacaatgc
    22201 actatccact ttgaatgccg ctgaatctgc cgcgcttgac gtagttgttg attgcttggg
    22261 tttgtcaatt gatacgggta atacctaccc gcaaaaaaac aaatacacaa acggcaagtt
    22321 tgtgattaac ggcaaaactg tcgatgttca ataccagcct atcagaagcg gtatcggtcg
    22381 attcatctcc ggaactggtg cagcagccaa cctcaaatcg aatgaatgga ctggcgcggg
    22441 tttaatcgtt attggcgaag gcgcaatgga gcagatggag aaatgtgttt cctcaatcgc
    22501 tattggcgac cgtgcgcagg gcttttctaa agtaagcagg gacaacatcg ccattggggc
    22561 cgacagcctg attaatgtgc aggccgctac tgaatggtac gaccagtcac gcatggaagg
    22621 cacgcgcaac atcggtattg gtggtaatgc aggacgcggc atcaccagcg gttactctaa
    22681 tgtgtcaatc gggcgcaatg ccggacaggg attgggtgaa ggctcgtcaa atattgcact
    22741 tggcgcaggc gcgatggctg gtactgctcc agtcggtttt agtggcgaca ttgaagtttt
    22801 ttggccgtct tcgacctcaa gaacaatcgc aatcggcgag gctgtcttgc aaacatatca
    22861 gggccgcgcc gctcaaaccg caattggtgc caatgcggcg cgaaatacaa aaaaggccga
    22921 aaaagttacc gcaatcggtt ctgccgcgat ggagaatctt gagcgaaacc gcgccccaaa
    22981 tggcggagat gttgtctaga cgggaacgga agcaggtacc tacgcccaat ctggaaaaaa
    23041 catcacgctt acatttccca acattcgcgg tgcgcaagcg acttattggg tgggcatccg
    23101 ccttacatca ggcacggcgc aaaccttaca aaacgacgtc gtaccggctc aggtcgtatc
    23161 agtgaatggc aatacattaa tcatccaaag ctcaaaagag ctgaccgcca ccggcgcggc
    23221 cgaactgaaa tacgtttatt ctgtaaattc aaccgctact aaaaacgaag agttgaccat
    23281 catcggcgcg aacgccatga ataaggcatt gaccgcagga tactcaacta tcatcggcgc
    23341 agatgccgcg ttgttgggag acaattatca aaaaacaacc gcaatcggcg catcatcttt
    23401 acgaacaggt agtcatattt ccacaactgc tattgggtat tgggtaatcc ctttggcaag
    23461 tagtgagaaa tgtgttgcca ttggagatag tgcgggctat cggaacgttc aaggcgactt
    23521 tttgactggg aaaataacaa actccatcgc catcggatat ggcgcaagaa taaacggcga
    23581 taacgaaatc caaatcggta cgacagggca aactttatat gctccaaccg cggtgaacat
    23641 ccgttctgac ggccgcgaca aagcagatgt taagccgttg acgaacggtt tagattttgt
    23701 aatgaagctc aagccgatga ctggctacta cgaccgccgg gattcctacg ttgacgaatt
    23761 attcaaagac ttgccggcag atgaacgagc ggacaaagtc cgcgaatggt gggcgaatcc
    23821 aatcaaggac ggcagtcata aagaagatcg gttgcggcat tggtttattg cccaggacat
    23881 tgctgcgctg gaagatgaat atggtcgatt gccgatggta aataaaacaa acgataccta
    23941 caccgtcgaa tacgaaacgt tcatccccgt tttgactaaa gccattcagg aaatggccgc
    24001 aagaattgaa acattagaaa ccgaaatgaa ggaatcgaaa aaatgacaag atgtgtgatt
    24061 gatcaagacg gcttgttcgt agaggaacag tattttgatg acggccgcca aagcatcgaa
    24121 gctgaaatgc ctgatctcgc acaatatcag gccgcccagt gggatggaca aggttggaag
    24181 ataattcccg actatcgcgg atgcgtagtt tttgccggtg ggcaagagca ggtgtgggat
    24241 aagttaggtg atttgcccga tggcgtcagc ctgaccccgc ctgaatcggt aaatattgac
    24301 ggcttaaaat ccgtaaaact cgtcgcatta aatgctgccg ctcaggcttt tattaacaag
    24361 cacgccggta tcgacagcgt acctgaattt gagtttgcaa gctgggcaat tcaagccgct
    24421 gaagcgaagg cttggcagga agataaagcc gcgccaacgc cagtgcttga cggcattgcc
    24481 accgcccgcg gtatgtcagc agacacgctt aaagcagcgg ctttgcgtaa aacgctggct
    24541 tacgaacaac tcgccgcaca tgtggcaggt caacgacaag cgctgcaaag caaaatcgaa
    24601 gcagcgaaaa cgcaggccgc gcttgataag attgcagtcg tattcacact gccggaggcc
    24661 gtctgaatgg ttcaagtcta tttggcactc tataaaggca aagccgcaat caacaccccg
    24721 cgcgatgtgg ttaaacgcat tgccgacagc gttgtacgat tggcaacatg cagcccgtac
    24781 agccattgtg aaatcgctgt taagcaccca cgcgacggcc tgtttgattg ttattcgtct
    24841 agcgcgagag acggcggggt gcgcattaaa accatgccgc tgcctgctga taaatgggac
    24901 ttaatcccgc tgccgcaatc tgttgccata tcggccagcc gcttgttcca ccgtacacac
    24961 ggtgcaggtt acgactggct aggtgcgatt ggcgtggtac tcaaatcacc acacagcaaa
    25021 agccgctggt tttgcagcga atggtgcgca tatgtaatag gctacactaa cccgtgccga
    25081 tacagcccgc aaaccctgta tgccgcggta tcaactaaag ataggccgtc tgagaaaatg
    25141 gaggaaacaa agtaatttag aaagttttaa ataaagagga gcggcgacgt gtctgtgttg
    25201 cgagcaccgg cacgccagcc aagcagatgt accctgcatt gacttcaagg ccgctttgcc
    25261 tagctaggcg gcggtaattc taacctaaac ggagttaatg cgacatggtt tattatcgtg
    25321 aattacgttg tgtctactgc aaaaaaacta ttggccaaag gcagcggtaa cgtacaaatc
    25381 aagtgtaacc gttgtaaaac ggttaatact ttcagctaga caaccattac taagaatgcc
    25441 gtcgagcatc attttaaaac tgatttcaga acaccagcga gagtgtcgga gagtaagtaa
    25501 aaatgatgca aaaataccac tcaacggccc cgctgccatt cgtcggacag aagcgatatt
    25561 tcattaaaca cttcactaaa gtattgtcgc aaattcccgc tgacggcaaa cattggacaa
    25621 ttgtagacgt attcggcggc agcggcctgt tggcgcacgt tgcaaaacgt atcaaaccgc
    25681 aggcgcgggt aatttacaac gactatgaca actactcaga ccgcctgcgg cacatcccag
    25741 attacaaccg tttgcgtgaa caaatcgcgc agatagttgg cggcatcccc aaaggctcaa
    25801 ggctagaccc tgaacgtacc cgatcagtgc aacaaacaat cactaatttc caaggccaca
    25861 ttgatgtgcg cgtactttca tcatggcttt tatttagtgc caaacaggca aattcgcttg
    25921 aacaattgct ggggtttgag ttctacaata aggtacgcca atccccatac tccatcgctg
    25981 ccgactattt agacggcctc gaaatcaccc agcaagacta taatcttttg atggctgagc
    26041 atcagcataa ccccaatact ctgctggtat tagacccgcc ttatgtgtcc actgctcagg
    26101 gtgcgtatgc cgctgataaa tactttaata tggttagctt cctgcgtatg attcagtata
    26161 tgcgcccacc gtttatccta tttagctcca cccgtagcga ggcgctggac tacttccaat
    26221 ttttgcaaga gtgcgaaccg gacaaatacc ggcgcttcag cggctacaat atcgtttcac
    26281 tagatgccaa gatgggcaaa ggaatcgagt atcaggacaa tatgatttat aaaatagatt
    26341 aacccgccta aacggcgggc ggtatcaaag ttccacctta tagcgcggcc taactaggtg
    26401 tcaaagattt tccgaaaagg tgctaaagtt cgcgccgcct tataaactgc acccgttttc
    26461 gggtgcagtt ttttattgcg gattgggacg ggaatggaaa aggatgtgga ttttttattt
    26521 taatcggcta tacaattgct gctttcatgc cgtctgaagc acgtttcaga tggcatcggt
    26581 ttacatcaga taaggataaa aatgggtatt tacgattttc aaatgaaaga tgcagaaggc
    26641 aatgcggttg atttgtcagg ctatcgcggc aaggttctgc tgattgtcaa cacggcaacg
    26701 cgttgcggtc tgaccccgca atacgaggct ttgcagaagc tgtatgcaca atataccgca
    26761 gaaggcttgg agattttgga ttttccgtgc aaccagttcc gcgaacaggc tcccgaaagc
    26821 agcggagaaa ttgcccaagt gtgtatgatg aagtttggca cgaagtttaa gattttcgac
    26881 aaaatcgaag tcaatggagc aaataccgcg cccttgtatg cctacctgaa atccgtcaaa
    26941 ccgcaagata aaggcaatca tctgtttaaa gatttcgtgt tgaagctcgc agctttggga
    27001 gagaagcgcg acgaaggcga catcaaatgg aactttacca aattcctcgt caaccgcgac
    27061 ggcgaagtgg tcgagcgttt tgcgcctagt gttacgcccg aagaaatcga agccgatatt
    27121 cgggcattgc tgtaatctgc cgccgtcttg ggaaatgccg tctgaaggct tcagacggca
    27181 tttgtccgtt tgcgggttta ttccgaaata tcggcaacac cgctttcggg gaagatgggc
    27241 aaatccggcg gtgggagcag ggtatccaaa gtcagggcgg acgcatcgag tgtggggtag
    27301 ccttcaaacg cgaccgaata accgctgttt ccgttcatac ggattcgggc gtgcgcgccc
    27361 agagggaaag tgattttgtc ggcaatgtgc ccgaagggaa agcccgtcag cacggggatt
    27421 ttcgccgtgc gcgaaacatg gttggcaacg gcggaaaaat cataagacgg atcatagaca
    27481 tctcgaattt tttccatacg gaaattgccg aacacgatgg cgcgctgttt cttcaaaata
    27541 cccgaaagat acagcgtatt gagcatacgt tcgatgcggt agggctgttc gccgacatct
    27601 tcgaggaaca aaatgccgcc gtcgatgtcg ggcatataag gcgtgccggc gagcgaggcg
    27661 aggacgctta agttgccgcc ccacaatatg ccttcggttt cgacatcggc gcgttggata
    27721 taaggaacat caacggtcag gcggttttgg gttgcaccct tgataaaggc atccatcgta
    27781 aacgcaccgg gggcgggttt gccaaaatcg ctataagcca tcgggccggc aaaactcatc
    27841 atattgcctt ttgccaacaa tgccagctgg acggcgcata cgtcgctgaa tccgaaaaac
    27901 agcgtgccgt gttcgcgcat ccttgcgccg agcgaagcaa aatcgatatg cggcagaatg
    27961 cgtgccgcac cataaccgcc gcgcaagccc atcagcactt taggtgtggc aacgcgaccg
    28021 gaagcgacat cttggaaatc ggcggcacgt tgtgagtccg tgccggcaaa ccgttggaaa
    28081 cggcggctgc ccgcctgttg gttggttacg gtaaaaccgg cattgtaaag gcgggttaag
    28141 gctgtgttga cgcggttggt gtcttcggca aagcctgacg aagcgacaac gcgcagaagg
    28201 ttgccgtccg aactttgacg gcgtggcgtt tggagaggca cggttcgggc tttcacaacg
    28261 gaatggaaag agggaagggg cggaacggat gtggcggatg tgccgcaagc ctgaagcagt
    28321 cccgcgccga tggcggcggt gcaggttttc aggaaacggc ggcgggaggt cggttcggtc
    28381 ataatttccc tttcatacgg tttcagacgg catcggcggg cttaaagcag ctttttaagc
    28441 tgttccgtcc aatcttgcgg cagggtcata ccgtgtttcc ggacggggtt tgcccaaatc
    28501 ggtgcaggga aagacgcatc gttttcaaag cgggcgataa tatgccaatg caggtgcggc
    28561 acgacattgc ccaagctggc gaggttaatc ttggcaggtt gaaacacttg gcgcatagcg
    28621 gcttcgactt tgtacaccat ttccatcaat tcgccgcgtt ccgctgccga aaggtcggtc
    28681 atttcggcaa tatgcttacg ccaaatgacg cggcagaatg caggcgaacc gctatcatta
    28741 tggacggcga tgacgcgcag cttgggggtt tgcagcaaaa cgtcttcatt ttgcgccgtg
    28801 cagatggggc agggttgggc ggtcattttt ccttattcct gaaagtgttt aaaaaacggt
    28861 agggtaattt ctgatatagc ggattaacaa aaatcaggac aaggcaacga agccgcagac
    28921 agtacaaata gtacagaacc gattcacttg gtgcttcagc accttagaga atcgttctct
    28981 ttgagctaag gcgaggcaac gccgtactgg tttaaattta atccactata gtttaatttg
    29041 tctgtccgca ttgccgctaa ttgtaccact tcatcgtatt tccttttcgg ttgaaacccc
    29101 gccctttagg gcggcaggat cagactttat ttgggagggg gtgtaacccc ttccaaatca
    29161 ggacgacaca tagggaggtg ctttatgtgt cgtcctgtgt gttgaaacat atcggacgcg
    29221 tttgaacagt tgtgcggcaa actgttttag cggcaggcaa gagatgccgt ctgaagccca
    29281 ttgtccgtca ttcccacaca ggcagttgct acggttgctg aagcccctta gccgtcgcgc
    29341 aggcggttgc tacggttact aaaatcccgt cattcccgcg caggcgggaa tccagtccgt
    29401 tcggtttcag ttgtttctga taaatcttgc agcgttgg
//
LOCUS       DAWFKN010000018        26619 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFKN010000018 DAWFKN010000000
VERSION     DAWFKN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.018"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(717..1523)
                     /locus_tag="WGR46_000797"
     CDS             complement(717..1523)
                     /locus_tag="WGR46_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0855777.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
     gene            complement(1565..1660)
                     /locus_tag="WGR46_000798"
     CDS             complement(1565..1660)
                     /locus_tag="WGR46_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0855778.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            complement(1672..2511)
                     /locus_tag="WGR46_000799"
     CDS             complement(1672..2511)
                     /locus_tag="WGR46_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0855779.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDWMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            complement(2511..3600)
                     /locus_tag="WGR46_000800"
                     /pseudo
     CDS             complement(2511..3600)
                     /locus_tag="WGR46_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3617..5680)
                     /locus_tag="WGR46_000801"
     CDS             complement(3617..5680)
                     /locus_tag="WGR46_000801"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0855780.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGVVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(5701..6111)
                     /locus_tag="WGR46_000802"
     CDS             complement(5701..6111)
                     /locus_tag="WGR46_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0855781.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(6188..7093)
                     /gene="glyQ"
                     /locus_tag="WGR46_000803"
     CDS             complement(6188..7093)
                     /gene="glyQ"
                     /locus_tag="WGR46_000803"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0855782.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEGASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            complement(7416..7838)
                     /locus_tag="WGR46_000804"
     CDS             complement(7416..7838)
                     /locus_tag="WGR46_000804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0855783.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(7849..9246)
                     /gene="atpD"
                     /locus_tag="WGR46_000805"
     CDS             complement(7849..9246)
                     /gene="atpD"
                     /locus_tag="WGR46_000805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0855784.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(9284..10159)
                     /gene="atpG"
                     /locus_tag="WGR46_000806"
     CDS             complement(9284..10159)
                     /gene="atpG"
                     /locus_tag="WGR46_000806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0855785.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(10184..11731)
                     /locus_tag="WGR46_000807"
     CDS             complement(10184..11731)
                     /locus_tag="WGR46_000807"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0855786.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(11742..12275)
                     /locus_tag="WGR46_000808"
     CDS             complement(11742..12275)
                     /locus_tag="WGR46_000808"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0855787.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(12280..12750)
                     /locus_tag="WGR46_000809"
     CDS             complement(12280..12750)
                     /locus_tag="WGR46_000809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0855788.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(12821..13057)
                     /gene="atpE"
                     /locus_tag="WGR46_000810"
     CDS             complement(12821..13057)
                     /gene="atpE"
                     /locus_tag="WGR46_000810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0855789.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(13114..13980)
                     /gene="atpB"
                     /locus_tag="WGR46_000811"
     CDS             complement(13114..13980)
                     /gene="atpB"
                     /locus_tag="WGR46_000811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0855790.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(13970..14323)
                     /locus_tag="WGR46_000812"
     CDS             complement(13970..14323)
                     /locus_tag="WGR46_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0855791.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(14691..15029)
                     /locus_tag="WGR46_000813"
     CDS             complement(14691..15029)
                     /locus_tag="WGR46_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855792.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(15165..16025)
                     /locus_tag="WGR46_000814"
     CDS             complement(15165..16025)
                     /locus_tag="WGR46_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0855793.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            16184..16753
                     /locus_tag="WGR46_000815"
     CDS             16184..16753
                     /locus_tag="WGR46_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0855794.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(16815..17678)
                     /locus_tag="WGR46_000816"
     CDS             complement(16815..17678)
                     /locus_tag="WGR46_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855795.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(17838..18524)
                     /locus_tag="WGR46_000817"
     CDS             complement(17838..18524)
                     /locus_tag="WGR46_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0855796.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRTIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(18526..19263)
                     /locus_tag="WGR46_000818"
     CDS             complement(18526..19263)
                     /locus_tag="WGR46_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855797.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            19793..21643
                     /locus_tag="WGR46_000819"
     CDS             19793..21643
                     /locus_tag="WGR46_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0855798.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIEAADLSA
                     SVPTRPVEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVVCYADLKHSDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDSNNAWRRVRGLLANSQTTDARNLAAALGSPFDGSTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVSDRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSHAALGNLQQAAKL
                     YEQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVRRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            21932..22144
                     /locus_tag="WGR46_000820"
     CDS             21932..22144
                     /locus_tag="WGR46_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0855799.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            22276..22722
                     /locus_tag="WGR46_000821"
     CDS             22276..22722
                     /locus_tag="WGR46_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0855800.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            22743..22892
                     /locus_tag="WGR46_000822"
     CDS             22743..22892
                     /locus_tag="WGR46_000822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855801.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(22921..23313)
                     /locus_tag="WGR46_000823"
     CDS             complement(22921..23313)
                     /locus_tag="WGR46_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0855802.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(23384..23989)
                     /locus_tag="WGR46_000824"
     CDS             complement(23384..23989)
                     /locus_tag="WGR46_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0855803.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAQLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(24091..25767)
                     /locus_tag="WGR46_000825"
     CDS             complement(24091..25767)
                     /locus_tag="WGR46_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0855804.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(25928..26542)
                     /locus_tag="WGR46_000826"
     CDS             complement(25928..26542)
                     /locus_tag="WGR46_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0855805.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
BASE COUNT         6387 a         6970 c         6971 g         6291 t
ORIGIN      
        1 aataacggaa ttttaagttt taggaatttg tcggaaaaac agaaatcccc cccgccgtca
       61 ttcccgcaaa agcgggaatc tagaaattta acgttgcggt gatttatcgg aaatgactga
      121 aactcaacgg actggattcc cgcctgcgcg ggaatgacga gattttaggt ttctgttttt
      181 ggttttctgt tctcgtggga ataacggaat tttaagtttt aggaatttgt cggaaaaaca
      241 gaaatccccc cgccgttatt ctcgcaaaag cgggaatctg gaaatttaat gttgcggtga
      301 tttatcggaa atgactgaac ctcaacggac tggattcccg cctgcgcggg aatgacgaat
      361 tttaggtttc tgttttcagg tttctgtttt tggttttctg ttctcgcggg aataacggaa
      421 ttttaagttt taggaatttg tcggaaaaac agaaatcccc cccgccgtca ttcccgcgca
      481 ggcaggaatc tagaatttta atgcggcaag aatttatcgg aaaaaaccga agtttaaaga
      541 cctagattcc cgcctgcgcg agaatgacga gattttaggt ttctgttttt ggctttctgt
      601 tttctcggga atcacgaatt agggcttacc cgaaaacaag tgaaaccgaa caaaccggat
      661 tccccgacat aaatttaggg acagcgcaca cccgccaaat aaggaaaggc caaaacctat
      721 tcaaacggga caacgtgccg cccgaggtgt ttttccaatt ttttctgacg tttccgttcc
      781 ctcgcccttt cgatttccct gccgattttt cccaaagcgc gtttcaacaa aaccttcggg
      841 gagcggcggt tgcggtacga ttcctgcctg ccgtttttca gttggctggg cagggtttcg
      901 ctttggacgc acaaggcggg ggtcagttgg caaaccgtca tccccccttt gtcgagaaaa
      961 ctgctgaaga gcatccaatc caccgccttc aggcgttcag acggcaaaac ggcaaacctg
     1021 tccaaaaaga aacgcatcgc ctttcgggaa ataatatagc ccgccatacc aaaatgttcg
     1081 ctttccaaca gcggaaaggc gcgcccgccg tagtccgcca cgccggaggg cgaggtcagg
     1141 acgtgcataa acatcgtttc caagcggacg ataaaggcgg aatccttgtc aaaacgctct
     1201 tgcagccaag cgtcttcggc aaggaatttc tccgcacctt cgccgagtaa aacgtcgtcc
     1261 tcaaatacgg cgatatacgg cagaccttca tccaatgcct gcttccacaa tacggcgtgg
     1321 ctcataaagc aggctttttc cactccgctc aaataggggt gcgccgacaa accgggtacg
     1381 agttccgcca ttgcctgctc cagtgcttca gacggcatca gtgcgtcgaa aaactgaaac
     1441 ggaatgtcgt gcctgccgaa ggtatcggca atgtgcgccc tgcgttctgc agcggaagcc
     1501 aagctgataa cgtggttttg cataatttat cctgtttttt gtctgttgga taaagcggcg
     1561 tttttcaacg gtttttcagc aatcggtgca aaatgccgaa gtattgcctc aaggtaaaca
     1621 gccgccgcat cctgccgtct gccgcaaata cgatgtccat cttttctcct tttattggaa
     1681 aggcacaatg atcttgccga ttaactgttc gcgcctttgc cgacgttttt ccctttccct
     1741 gctgattttg gtcaaggcgc ggatcaggcg gtgtttgaat gtgttggcgg gggaatcgcg
     1801 cctttgctgt ttgcggttca ggaggcggtc gtgttcgatc aggctgccca atgcgctgtt
     1861 ttggtcgtga aacttggcat aatgcagctc ttgggcgcac aaggcgggat cgagctggca
     1921 aaccggcatt ccttccctgt cgaaaaaatc gctgaacatc atccaatcga cggggtgcag
     1981 cccttcgggc ggcagggcgg caaacctgtc caggaaaaac cgcatcgctt ttcgggaaat
     2041 gatatagccc gccgtccccc agtgttcgct ttccaacagc gggaaggcgc gcccgcagta
     2101 gtccgccacg ccggagggcg aggtcaggac gtgcataaac atcgtttcca agcggacgat
     2161 aaaggcggaa tcggggtcaa agcgttcttg cagccaagcg tcttcggcaa ggaatttctc
     2221 cgcgccttcg ccgagcaaga catcatcttc aaatacggcg atatacggca gaccttcgtc
     2281 caatgcctgt ttccacaata cggcgtggct cataaagcag gctttttcca ctccgctcaa
     2341 ataggggtgc gccgacaaac cgggtacgag ttccgccatt gcctgttcca gcctttcaga
     2401 cggcatcagt gcgtcgaaaa actgaaacgg gatgccgcgc acgccgaagg tatcggtaat
     2461 gtgcgccctg cgttctgcgg cggaagctaa gctgataacg tggttttgca ttaaatctcc
     2521 tgttctttcc ctgccgaatc cgaccgcgcc tgccggcggt ttttaatcag ccggtgcaaa
     2581 atgccgaagt attgcctcat ggtaaacagc cgcctcatct tgccgtctgc cgcgaaatcc
     2641 agccacgcgc cggcgggcgg cgtgtccgtc cgtttgaagc attggtacaa aaaccggcgg
     2701 gcgcgttcaa aatcttcttc cggcaaatgt ttctccagca attcatacgc tactgctttt
     2761 atttggcggt attcaaggct gtcgaaccgg gttttaaaac ccatagactg caaaaaatcg
     2821 tttctggcgg ttttttggat gccttgcgcg atttcgtgtt ggcggatgct gtatttggat
     2881 gaaacctgat tggcgtgaag gcggtatttg accaaggctt cgggataata agccagcctg
     2941 cccaatttgc tgacatcgta ccaaaattgg taatcttccg cccaatcccg ctcggtgttg
     3001 taacgcaatc cgccgtcaat gacgctgcgc cgcataatca tcgtgttgtt gtgtatgggg
     3061 ttgccgaaag ggaaaaagtc ggcaatatct tcgtgccggg tcggcttttt ccaaattttg
     3121 ccgtgcctgt gatgccgcgc cagccggttg ccgtcctttt cttccgacaa aacttccagc
     3181 cacgcgccca tcgcgatgat gctgcggtct ttttccatct cgcccacgat tttctcaatc
     3241 cagtcggggg gcggcaatat cgtcggcatc ggtgcgtgca atatattccc ccattcctga
     3301 ctttgccaat tcgtccagcc cgatgtttaa agagggaatc aggccggaat tttgagcttg
     3361 tgcaaggatt ttgatacggc tgtcccgctt ttgaaaatcc ttggcaatgg caagcgtacc
     3421 gtctgtcgag ccgtcatcga caatcaaaat ctccaagttg cgccaagtct gattcacgac
     3481 ggtggctaat gattgggcga aatatttttc tacgttgtag gcgcaaatca atacgctgac
     3541 taaaggctgc aatttattct cccgataggc acgatgccgt ctgaaggctt cagacggcat
     3601 ttggactgta caacggttac tcgcccaaaa gcgcgatgtc ggctaccgcg ttcatttgct
     3661 ctgccaagcg gttcagcagg ttcaggcggt tttgttttac ggcggcatct tccgccatta
     3721 ccatcacgcc gtcaaagaat gcatcgactt gcggtttgac ggaagccagt tcggacaagg
     3781 cggtttggaa attgccttcg gcaacagcgg cggcgatttt cggctgcaag ccttgcgcag
     3841 cggcaaagag ggctttttct tcgtcctgtt gcaacaggct ttcgttaacc acgcccaact
     3901 cggcatcggc ttttttcagc aggttttgca cgcgtttgtt ggcggcggcg agcgcggcgg
     3961 cttcgggcag ctgtttgaac gcggcaacgg cttgcagttt ggcggtcaaa tcgtccaaac
     4021 ggcgcggctg tttcgccaat acggcggcaa cgatgtcttg cggataatcg ttttgcagca
     4081 acacggccaa acgcgcctgc ataaagtcgg cggtttcaga cggcgttttt tcgttgagca
     4141 aacctttggg gaagctgtcg aatgccgtct gaatcagttc gttcacgtcc aaaccatact
     4201 gcatcagcat acgcaaaata cccaaggcag cgcggcgcag ggcgtagggg tctttgtcgc
     4261 cggtcggaat cagaccgatg ccccaaatgc cgaccagggt ttccagcttg tcggcaagtg
     4321 caacggcagt ggcaattttg ctttcgggca gcttgtcgcc ggcaaaacgc ggttggtaat
     4381 gttgctcgac ggcttcggcg atttcttcgg tttcgccgtc caagcgggca tagtatttgc
     4441 ccatcgtgcc ttgcagttcg ggaaactcgc cgaccatttc ggttaccaag tcggctttgg
     4501 caagacgcgc ggcgcgttcg gcagcggcgg catccgcgcc caatgctttg gcgatgtggg
     4561 cggcaatgct ttgcaggcgt tcgatgcgtt cggcttgcga gccgattttg ttgtggtaca
     4621 ccacgctgga caatttcggc aggcggcttt ccaaagtcgc tttttggtct tgtttgtaga
     4681 agaactcggc atcagacagg cgcgcgcgca aaacgcgttc gttgcctcgg atgatgtgcg
     4741 acgggtcttc ggtttgcagg ttggaaacca gcaggaagcg gttcatcagc ttgccgtttt
     4801 ggtcgagcag cggaaagtat ttttggtttt gctgcatcgt taaaatcaag cattcctgcg
     4861 gcacggcgag gaaatgttcc tcaaaaccgg cttccaaaac aacaggccat tcgaccagcg
     4921 cggttacttc gtccaacaat gcttcatcgg cggcgacggc ggcgttcaga ctgcgcgcct
     4981 gcccttccaa tgccgtctga atcgcggctt tgcgctcggc aaacgaagcg acgactttgc
     5041 cttgcccgcg catttgttcg gcgtagctgt cggcgttttc aatggtgatt tcgccgttgg
     5101 acaagaagcg gtgtcccaag gtttgattgc tgctttgcag acccaaaacg ctgacgttta
     5161 cgatgtcgcc gccgtgcagc acaatcagcc cgtgaacagg gcgcacgaag gtaaatgtgc
     5221 tgctgcccca acgcatcact ttgggaatcg gcagtttctt aaccacttga ttgataatgt
     5281 cttccaaaag tccgcccagc ggcttgccgg tttggacgta ttcgtaggcg tacacatcct
     5341 gcttgccgtc gtggacgatg gtcaagtctt cgattttcgc gcctgcaccg cgtgcgaaac
     5401 cttccaaagc cttggtcggc gcaccgtctt ttacggcatt cgccacggca gggccttttt
     5461 tcacgatttt ttggtcggcc tgaacggctt tgacgttttt gacttgaacc gccaaacggc
     5521 gcggcgaggc ataagccgta aattcggctg caccgtcaac cagttgcgct ttttccaagc
     5581 cttcggcaac ggcggcggca aaatggttgc ccagattatt cagggctttg ggcgggagtt
     5641 cttcggtaag gagttcgatt aaaagggttt gggtcatcat tcggctttct ttgaatttgg
     5701 ttaatctgcc tgtttatagg tttcgctgta attttcccag ccgtcatccc cgtaaaaacc
     5761 atcaaccagc ggggtggcgt acaaagtggc gacatcttcg cggcctgcca gccaagagat
     5821 aatggctttt ttctcggttt cgcccaagct tcgggcaccg gatttttgaa acaggcacga
     5881 aaaatcgccg caatcgcccc cgcccgccat ttccaagcca tttgcctcaa gatacgcaat
     5941 cagctcgtcc ataaagcggc caaacgcttc ggcatcgtcc gcagcttggt gcaaactgcc
     6001 ttgaacgccg aaaatcaatg tttgaaactc gcccaaatgc agctttttat gctggcggcg
     6061 gttcattttg tgcaggcgtt tcctgcttgg ggtgcggaaa tagacaggca tgattttcct
     6121 aaaaaatata atggcttccg gacggctgcc ttatcgtgcc gcccgaacgt aaaaaatcgt
     6181 cgccccctta ggcagcattt gccttcatta aagggaagcc cagtttttcg cggctttcaa
     6241 catatttttg cgccacggcg cggctcaatg cacgaatacg tccaatataa gttgcccgct
     6301 cagttacgga aatcgcgccg cgcgcgtcca agaggttgaa cgtatgcccc gctttgagaa
     6361 ccagctcgta ggcaggcagc gcaaggcttg ctccttcaac ggcaagcagg cgtttggctt
     6421 gcgcttcgta gtcgttgaac tggcgcagca gccaatcggc atcgctgtat tcgaagttgt
     6481 aggtggattg ttcgacttcg ttttggtggt acacgtcgcc gtaggttacg gtattgccgt
     6541 ccagcgtttt tgcccaaacg aggtcataga cgttttccac gccttgcagg tacatcgcca
     6601 agcgttcgat gccgtaggta atttcgccga gtacgggggt gcagtcaatg ccgccgactt
     6661 gttggaagta ggtaaactgg gttacttcca tgccgttgag ccagacttcc cagcccaaac
     6721 cccacgcgcc caaagtcgga ttttcccagt cgtcttcgac aaaacggatg tcgtggactt
     6781 tggggtcgat gcccaattcg cgcagagaat caagatagag gtcttggata ttggcggggg
     6841 cgggtttgag ggcgacttgg aattgatagt agtgttgcag gcggttggga ttgtcgccgt
     6901 agcggccgtc tttagggcgg cggctgggtt ggacgtaggc ggcaaaccaa ggctcggggc
     6961 cgagcgcgcg cagacaggtg gcggggtggg aagtacctgc gccgacttcc atgtcgaagg
     7021 gttggatgac ggtgcagcct ttgtctgccc agaatgtttg cagtttgaag atgatttgtt
     7081 ggaaggtaag catggcttat gattcgataa aataaagggt ttattttact gtttccattg
     7141 ctgtttggat aggtttatct caaagacaga ctgatttgaa aacacggcat acatgatata
     7201 gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct
     7261 aaggtgctga agcaccaagt gaatcggttc cgtactattt gtattgtctg cggctttgtc
     7321 gccttgtcct gatttttgtt aatccactat attttcaaaa cttggaaaaa taaaaagcac
     7381 gaccaaacgg tcgtgctttt ccaaaccaaa caagtttatt tcttgtgcga acggatatag
     7441 tccaaagttt tgagctgtgc aatcgcagca gccaatgctt tatgtgcttc cgccaaagcc
     7501 ttatcgtctt tagcttggga aatgcccgct tctgcggctt ttttcgcctc ttccgcacgt
     7561 gcctgatcca tctccgcact gcggacggca acatccgcca agacagttac tttatcaggc
     7621 tgtacttcca aaacaccgcc ggaaacagca accaaaacct ctttatcctc gcccggaacg
     7681 gtcaaacgca aagcccccgg ccgcaccaaa ctcataatcg gctcgtgtcg cgggtaaata
     7741 ccaagctcac cttgaacggt cggaaccact acaaagctgg cttcgcccga atagatgttt
     7801 tgctcgctac tgaccacctc aacttgcatg atgctcatgc cgacctcctt agtttaaggt
     7861 tttcgctttc tctactgctt cttcaatgct gccgaccata tagaatgcct gctcgggcag
     7921 atgatcgtat tcgccgttca agatggcttt gaagccggca atggtatcgc gcagggcgac
     7981 atatttgccc ggagaacctg taaacacttc ggcaacgtgg aacggttggg acaggaagcg
     8041 ttggatttta cgcgcacgca ttacggtcag tttgtcttcg tcagacaatt cgtccatacc
     8101 caagatggcg atgatgtcgc gcaattcttt gtatttttgc agggtggact gcacaccgcg
     8161 cgctacgtcg tagtgctctt gacccaatac catcggatcc agttggcgcg aagtagaatc
     8221 aagcggatcg actgccgggt aaatacccaa agaggcaata tcgcggctca atacgacggt
     8281 tgcgtccaag tgggcgaacg ttgttgccgg agacgggtcg gtcaagtcgt ccgcaggtac
     8341 atatacggct tggatggaag taatggaacc ggtttgggta gaggtaatac gctcttgcaa
     8401 acgacccatt tcttctgcca atgtcggttg gtagcccact gcagacggca tacggcccaa
     8461 cagtgcagat acttcggtac cggccagggt gtaacggtag atattgtcca cgaagaacaa
     8521 tacgtcgcgg cctttgccgt tttcgtcttt ttcatcacgg aagtattccg ccatggtcag
     8581 accggtcaat gcgacacgca aacggttgcc cggaggttcg ttcatctgac cgtaaaccat
     8641 tgccacttta tccaatacgt tggaatcttt catctcgtgg tagaagtcgt taccttcgcg
     8701 ggtacgctca cccacgcctg cgaacacgga caagccgctg tgcgctttgg cgatgttgtt
     8761 gatcaattcc atcatgttca cggttttacc cacaccggca ccgccgaaca gacctacttt
     8821 accgcctttg gcaaacggac acagcaagtc aatcacttta atgcccgttt cgagcaattc
     8881 ggttgtggaa gacagttcgt caaacttagg ggcggcttgg tggatggcac ggctcttgtc
     8941 ggtatcaatc ggacctgctt cgtcaacagg cgttcccaat acatcgacaa tgcgtcccaa
     9001 cgtaccttta cctaccggca cagtaatggg cgcaccggta ttgctcacag tcatgccgcg
     9061 tttcaaaccg tccgagctgc ccatcgcaat ggtacggact acgccgtcgc ccaaaagctg
     9121 ttggacttcc aaagtcagac cgttttcgtc taatttcaaa gcgtcgtaaa cgcgcggaat
     9181 catgtcgcgt ggaaattcca cgtcaacaac cgcaccgata atttgtacga ttttgccttg
     9241 gctcattatc gtatcctaat ttctgtacag gattcagacg gcatcagaca gccgccgcac
     9301 ctgctacaat ttctgacaat tccgtggtaa tcgcagcttg acgcgatttg ttatatacca
     9361 gacgcaactc tttgatggca ttgcctgcat tgtctgttgc agctttcatg gcaaccatgc
     9421 gggctgcctg ttcggatgcc atattgtcgc tcaacgcctg ataaaccaca gactctaaat
     9481 agcggcgaac cagatattcc aacactgcaa gtgcagtcgg ttcgtagcgg tattcccagc
     9541 tgaacggtga tttgggagct gaatcgccaa tcacgttctc accgataggc agcaatactt
     9601 ccattctcgg ttcttgacgc atggtattga caaaacccga atacaccaga tggattctgt
     9661 caatttcatg tttctcatac cgttggaaga gttctgtcaa aggtccgagc agcatttcca
     9721 tttttggggt atcgcccaaa tttacggcac tggcaaccac attcagacca atgctctgac
     9781 acgccatcag acctttactg ccaaggcata cgatttcctc ttcaatacct tgattccgat
     9841 actcttgaac ttgtgccaaa aactttttca gcacgttggc gttcaaaccg ccacacaaac
     9901 ccttatcaga cgtaatcaaa ataaaaccga cacgtctaat ctcccgatga gattccagta
     9961 acggaatacc atgatcggta ttggtttgcg caagatggct catcaccata cgcacttttt
    10021 cggcatacgg acgcgccaaa cgcatccgtt cctgagtctt ccgcatttta gaggttgaca
    10081 ccatctgcat cgctttagtg atcttttggg tattctgaac actgcggatt ttggtgagaa
    10141 tctcttttcc tactgccatt tcagactcct ttcacttcaa gccttatgcc tgataggcgt
    10201 aagaagattt gaaggatttc atggctgctt caagcgtttt ctcgctctcg tcggacattg
    10261 cacctgaagc attgacggct tccaaaactt ccggatgttg ggtacggaca aagctcaaaa
    10321 attcagattc aaaagccaga gctttggcaa ccggaacatc agaatacgaa ccgttgttga
    10381 ttgcccaaag ggtcaaagcc atttcagccg tattcaacgt actgaactgt ttctgtttca
    10441 tcagttcggt tacgacttcg ccatgctcca attgtttgcg cgtagcttca tccaaatcgg
    10501 atgcaaattg cgagaacgcc gccaattcac gatattgtgc caacgccaaa cggataccgc
    10561 cacccagctt tttaatcact ttggtttgtg cagcaccgcc tacgcgggat acggaaatac
    10621 cggcattgat tgcaggacgg ataccggcgt tgaagaggtc ggtttccaag aaaatctgac
    10681 cgtcggtaat cgaaatgacg ttagtcggaa cgaaagcaga tacgtcgccc gcttgggttt
    10741 cgataatcgg caacgcggtc agagaaccgg ttttgccttt tacttcgccg ttggtcaatt
    10801 tctccacttc gtgttcattg acacgtgccg cacgttccaa cagacgggag tgcaggtaga
    10861 acacatcgcc aggataggct tcgcggccgg gcggacggcg caaaagcagg gaaatttgac
    10921 ggtaagccac agcctgtttg gacaaatcgt cataaacaat caaggcatct tcgccacgat
    10981 cgcggaagaa ttcacccatc gtacaaccgg agtaaggtgc gatatattgc aatgccgccg
    11041 cttcagatgc agttgcagca accacaatcg tgtgttccat cgcgccatgc tcttccaatt
    11101 tgcggaccac gttggcaata gaagatgctt tttgaccgat agcgacatag atacagataa
    11161 cacccgtacc tttttggttg acgatggcat ccaatgctac ggccgtttta ccggtttgac
    11221 ggtcgccaat aatcaactca cgctgaccgc gaccgacagg aaccatagag tcaatcgcct
    11281 tcagaccggt ttgcatcggc tggtcaaccg atttgcgcgc aatcacgccc ggtgcgattt
    11341 tttcgatagg ggcggtcaaa gttgtattaa tcgggccttt gccgtcgata ggccgaccca
    11401 atgcatcaac gacgcgtccg accagttcgc gtccgaccgg cacttccaag atacgaccgg
    11461 tacaggtaac cgtgtcgcct tctttaatat gttcgtactc gcccaacact acggcgccga
    11521 cggagtcgcg ctccaggttc atcgccaagc cgaaagtgtt acccgggaat tcgagcatct
    11581 caccttgcat tgcatctgac aaaccataga tgcgaacgat accgtcagtt accgaaatca
    11641 ccgtaccacg ggtacgcact tcggcattta cagacagatt ttcgatcttg gctttaatca
    11701 aatcgctaat ttcagcagga ttaagctgca tgaaaactct cctaattcgt catagtcgtg
    11761 tacaaggcac tcaatttgcc ttgtacagac aaatccaaaa cctgatcacc cacttcaact
    11821 tttatgccgc caatcagctc cggttcgatt tcgacagaga ttttcagctc gctgtcgaaa
    11881 cgcttattca gcatttgcgc caactcgccg acctgtttgt cggtcaacgg ataggcactg
    11941 taaatgacgg cagatttgat atggttgaat gataaggtca agtcttgata ttgagcatat
    12001 acttccggca atatcgacaa acgtttctgc ccggccaaga cgataacaaa gtttttcaac
    12061 tccttgtctt tcaaaccgac caaatcgatg aggatatctg ctttttctga agcattcgtt
    12121 tcaggacggt caatcaatga agccaccttc ccttcctgaa caaccgccgc aagtttttcc
    12181 agtccgccca accaagactc aatttggctt ttttcctgag ccagaccgaa caatgccttt
    12241 gcataaggtc tggcaatcgt tgcgaactct gccataagat tacagctcct gtttcagggt
    12301 atcgagcagt tttgcgtgtt tggaagcatc gacttcgctg cgcaaaatag attcggcacc
    12361 tttgacagcc aacacggcaa cctgctcgcg cagggattcg cgtgcgcgga acaattcctg
    12421 ctccacatcg gcctttgcct gagctgcaat gcgcgccgcc tcggaagaag cctgttcttt
    12481 ggcttcttcg acaattttgg cggcacgttt ttcggcgttg gcaaccattt cggaaacctg
    12541 attacgccct tctgccaaga gttctgcaac ctttttttca gcctgctcga aatcgctttt
    12601 accacgctcg gcagctgcca agccttcggc gacttttgcg gcacgctcat ccaaagcttt
    12661 tgcaatcggc ggccacacga atttcatggt aaaccatacc aaaccgaaaa agacgatgat
    12721 ttgagcgaat aatgttgcat tgatattcac gttacttaac cttcgtattg gggttaatca
    12781 aacaggctgc gcctgtacgg aacggacgaa tccgtcctga ttatgcacct gcaaacgggt
    12841 taacgaaggc gaacagcagt gcaatggcga caccaatcaa gaatgcggca tcaatcaaac
    12901 cggcaatcag gaacagtttg gtttgcagcg gaccgatcag ttcgggctga cgggcagaag
    12961 actccaaata tttagaaccg accattgcga taccgataga ggcacccagt gcgcccaatg
    13021 caacgatcaa accacatgcg atagcaatca aacccatttt aaactcctta aagaaacaaa
    13081 ggttaaacta caaaaacaaa ctacttagga aaatcagtgc gcatcatgtg cctgtccgat
    13141 atagacgaac gccaacgcca tgaaaataaa cgcctgcagg gtaatcacca aaatatggaa
    13201 aatcgcccat gccaaaccgg caataatgtg gaatacaaac agaatcggat ccatgacttc
    13261 gacgctgccg gaagccgccc aagcaccgcc aagcaaggct atcaacaaga ataccaactc
    13321 gcctgcatac atattgccga acaaccgcat accgtgggat acggttttag aaagaaactc
    13381 gaccaaattc aacagaaagt tcgcaggtgc gagttttgca ccgaacggcg cgctgaacaa
    13441 ctcgtgaaac cagccaccca atcctttgat tttgatgttg taatagatac aaatcagcaa
    13501 cacgccgaca gcgagtgcca aagtggtgtt caaatcggca gtcggtacga cgcgcagcag
    13561 ggcgtgatgg ttgccggtaa tgccctgcca taccatcggc agcaaatcga ccggcagcat
    13621 atccatcgcg ttcatcagaa aaatccagac aaacagcgtc agacccaacg gcgcgacggc
    13681 ttttctagac ttttcgttgt gaatgatgct cttacacata tcgtccacaa actcaaacaa
    13741 gatttccacg gcagcctgga aacgtccggg aacgcctgcc gttgcttttt tcgcaccgcg
    13801 ccacaacagg aagctgccga ttacgcccaa caggacggca aaaaagacgg catcaaggtt
    13861 aataaacgaa aaatcagcaa tgtttttcag tccctgaccc tgagtaacat ccgacaaact
    13921 ggtcaagctc tgcaagtggt gcttgatgta gtcggcagcg gtaatggttt cacctgccat
    13981 aatctttcac tctcaacaat actaaaaaaa ccaaatggct gacaccgagc agccccatca
    14041 gaaacggggc gaacaccaac gattgatgcc atacagcaaa tacggcgagc atggacaaca
    14101 gcgacagcac tacttttaaa atctctccga agacgaacat cctgctttgc aggaaagggt
    14161 ttcccctgaa aagttttaaa agtaaaactg caacaaacgt gggaagcagg taggacaaac
    14221 cgccaccgac cgccgaaagg aatccggcaa aaccccatac agcaaaggca actgcggcgc
    14281 atatggacaa tacggcggat tgtaggatga taatctgctt cataaaggga atgtttccgc
    14341 ctcggatttg gggcgcggct aatataattt agaagcctta ttacgtcaag cgacagttaa
    14401 tctttgtgaa acaacgtatc ccaatccgcc gcgctcgccg cctgaataac ggcgacaggt
    14461 gtcattctaa cacacattac atataattac aggatattaa ggagtttgtc cgcaatttct
    14521 ttacattttt aatgttctta cgtgatttgt ttgctttacg tggaaataat aaaaaatcaa
    14581 cgcgaaattg tagcagttta tcggtcggat tgtcggcagt ttggggaatt tgctcaataa
    14641 ataaaaggtc gtctgaaaat attttcagac gaccttttcc gaataaagga ttagcaacgg
    14701 cctgccgctt taagcaaagc attgcattga cttttgcctt tgtgcgttcc gcctcccaaa
    14761 caaattgcat cggaagtggt aacgccgatt gtgctgatta cactggtaac atagcattgg
    14821 ctcacgcgct tacccacagt tgcggtaaag ttgatgcgta tgccttcatt gttgcggttg
    14881 ctgattttta cggcatttgg gctgacgccc aaggcaaacg cggcacgttc ctgaagtttc
    14941 tggtcggaaa cggttacatt attgattgag ccgcaacctg ctaatgccaa cgcaacgaac
    15001 gcagccgaaa cgatgatgcg tgtgttcata atttcctcga aaattaaaaa tgaaaacagg
    15061 aaaacgattc ttacgtgaag cagaaaaaat gtcaatagaa ttatatttcc cacttaaaat
    15121 ctggaaagct attctctata tttcagacgg tatatcccgc aaaattaagg tcggtaatct
    15181 atgcccaatt gcttcaagat atggtcgaag gtttcgggcg tgtcgaaatg caggatgatt
    15241 ttgccttttt tatggttggc ggttttgatt tcggcgttga cacccagttt ttcagtcagc
    15301 aaatcattca ggcggccgat gtcggcggcg gcagtctttt tgggctcggg acgtttgttt
    15361 tgaagggcgg cttggctgcg gcgttcgact tcgcgcaccg accagccgtt tttgacggcc
    15421 ttttgcgcca attcgagctg ttcgacgacg ggcagggtca gcaatgcgcg ggcgtgtccc
    15481 atttcgaggc ggcgttggta aagcatttcc tgcacgggtt cgggcaggct taaaaggcgc
    15541 aggctgttgg aaatcgcgct tcggctttta ccgacggcct gggcgatggt ttcgtgggtc
    15601 agtccgaact cgtcggcaag gcgtttcaag ccttgtgctt cttcgatggg gttgaggttt
    15661 tcgcgctgga ggttttcgat caaacccatt gccaatgcgg tttcgtcgct gatggttttg
    15721 ataacggcgg ggatttcggt caggccggca atctgtgcgg cacgccaacg ccgctccccc
    15781 gcaatcagtt cgtatcggga cagtccgtgt tcgcgcacga tgacgggctg tatcacgcct
    15841 tgcgccttaa tcgaatccgc cagctcctgc aaggcttcgt catcgatttg aacacgcgcc
    15901 tgatagcggc cgggccggat atctttaacc gcaaccgtgg tcaatcggtc gccgctgctg
    15961 ttgtctgcgc cgttggcgag cagcgaatcc aaaccgcgcc ccaatccacc ttttactttt
    16021 gccataccgc cctcccgtgc ctatttcaga taggatgtta aatcggatat tttatcggat
    16081 attgggtgtt gccgacaatt tgtatccgtg tttatcggat ttctgttttt tcactataat
    16141 agctggtttg ccgttgcagg cggttttatg ggaaaggcgg atgatggtac ggcgtttgat
    16201 aatcggcatc agtggggcga gcggtttcca atacggcgtg aaggctttgg aacttttacg
    16261 cgcgcaagat atcgaaacgc accttgtggt atcgaaaggt gcggagatgg cgcgcgcttc
    16321 ggaaacggct tatgcgagag acgaggtata tgccttggcg gacttcgtgc atccgatcgg
    16381 caatatcggg gcgtgcattg ccagcggtac gtttaaaacg gacgggatgc tggtcgcccc
    16441 ctgttcgatg cggacgcttg cctcggtcgc gcacggcttc ggcgacaacc tcttgacgcg
    16501 tgcggcggat gtggttttga aggaaaggcg gcggctggtg ctgatggtgc gcgaaacgcc
    16561 gctgaacctt gcccatttgg acaatatgaa gcgggtaacg gaaatgggcg gcgtggtgtt
    16621 tccccctgtt cctgcgatgt accgcaaacc gcagacggcg gacgacatag tggcgcacag
    16681 tgttgcacac gctttgtcgc tgttcggaat cgatacgccg gattcggcgg aatggcaggg
    16741 aatggcggat taaaggacaa aaatgccgtc tgaacacgga tacggttcag acggcatcat
    16801 tttatacgac tgccttattt ggctgcgcct tcattccatg cggcagggga tttgtagccc
    16861 tcgaagcgtt tgtgcgcgta ggctttgaac gcgtcggagt tataggcctc ggttacgtct
    16921 ttaagccatt ggctgtcttt gtcggcggtt ttgacggcag accagttgac ataggcaaag
    16981 ctcggttctt ggaacagggc ttcggtcagc ttcatgccgc tgcttatggc gtagttgccg
    17041 ttgacgacgg caaaatccac gtcggcgcgg ctacgcggca gttgcgcggc ttcaagctcg
    17101 acgattttga tgtttttcag gttctcggcg atgtccgctt tggatgcggt cagcggattg
    17161 atgccgtctt tgagtttgat ccaacccagt tcgtcgagca tcaccaagac gcgggcgaag
    17221 ttggacgggt cgttgggcgc ggatacggtg ctgccgtctt tgacttcttc cagcgatttc
    17281 agcttgcccg ggtacagtcc caaaggcgcg gtcggcactt ggaagacttc ggtgatgtcc
    17341 agattgtgtt cttttttgaa gtcgtcaaga tagggtttgt gttggaagac gttgatgtcc
    17401 aactcgccct cagccaatgc cagattcggg cgtacatagt cggtaaactc gaccagtttg
    17461 acggtgtagc cttttttctc cagctcgggt tggatgtgtt ctttgaccat atcgccgaag
    17521 tcgccgacgg tcgtgccgaa gacgatttct tttttctccg cgccgttgtc ggcggcggca
    17581 gaagcggatg cggcgggcgc gctgtctttt tgaccgccgc aggcggcgag gatgagcgcg
    17641 agtgcggcgg cggaaagggt tttgaagaag gttttcatat tttctcctga tgttgtggca
    17701 gtttcaaaca aaaatgacgg gcagggagtc ctgccgtccg gattcggcgt tcagacggca
    17761 tttgccgcga acagggggat tttatagcat ttttcggata gcggtggggg ttttggcgtt
    17821 cagacggcat tttgggttca acgtttgtcg agtttccgcg ccaacgcgtt gccggtgctt
    17881 tgaatcagga taacgagcag cacgaggagg gcgacgatga agacgatgac ttcggtttgg
    17941 tagcggtagt agccgtagcg gatggcgagg tcgcccaagc cgccgccgcc tatcatccct
    18001 gccgccgcgc tgtatgacaa aagcccgatg gcaagcacgg taatgctgga aaccatgccc
    18061 gcgcgcgctt cgttcaagag gactttgcag acgatggcaa tcggaggcgc acccatcgcg
    18121 gcggcggctt caatcacgcc tttggggact tcgcgcaggt tttgttccac cagtcgggca
    18181 aaataaaaca atcccgacac gctcaacacc agcgaggcgg caaccggacc gatggtgctg
    18241 ccgacgatgg tgcgggtcgc gggtatcatg gcaatcatca ggatgacgaa ggggaaggcg
    18301 cgcatgaggt tgacgaggtt gtcgagcagg aagttcaccg gcttgttgta atgcagttgg
    18361 cggctggaag ttacgaagag caacacgccc agcaacgtgc cgaaaacgac ggcgaatgtg
    18421 gtggacaagc cgaccatcac gaaggtttcg cccaatgcgc ggacgatttc gcctttcata
    18481 ccgatgatgt tggaaacggc ttgttggaag gtcaggtctg ccatttcagt cctctctgat
    18541 gagttcgcgc ccgatgtcgg atcgggcgtg gatttggttg ccgcgtactt cgacgatttc
    18601 gacgactttt cccttatcca acagggcggc tcggtcgcac agtcgtctga tgacgctcat
    18661 ttcgtgggtt acgatgacga tggttacgtt gaagcgtttg ttgatgtctt ccaaacattc
    18721 caagacgctg cgcgtggtgg cggggtcgag ggcggaagtg ggttcgtcgg cgaggatgac
    18781 ttgcggcttg gatgcgagcg cgcgggcgat gccgacgcgc tgtttttgtc cgccggaaag
    18841 ctgggcggga tagtgaccgg cgcgttcggt cagtccgacg atttcgagac attcggcaac
    18901 gcgcgctttg attttttcag acggccatcc ggcgatttcc aatgggaagg cgacgttgcc
    18961 ggcgacggtg cggttgttca acaggttgaa ctgctgaaac accatgccga tattctgccg
    19021 agcctgacgc aatgcggcgg catcaagcgc ggtcagctct tgtccacaga cgttgacctt
    19081 gcccgtgtcg ggacgttcca acaggttaat cagtcgcaac agggtggatt tgcctgcacc
    19141 cgaataaccc atcagcccga agatttcacc gtcgcggatt tcgaggctgg tcggctcgac
    19201 agcggcaaaa cgggtcttgt cgcgcgtttg gtaatgcttg gaaaccttgt ccaaaataat
    19261 cattgtcttt cccatacaac aaagcccgat gtcggacaca acgggcgcgg aagataaagc
    19321 tgaaattgtc ggaacgcttt agctgttatg cccgcaagct gtgtcaaatc ggcaggttaa
    19381 ttttcgtagg atattatcgg gaaagcgttt ttttgtcaat aaggcaggaa gcgggcaacc
    19441 atttcggaca atgccgtctg aaacaggcaa aggcagcggt tcgcactaaa acggcaaata
    19501 attgaaaaat atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
    19561 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
    19621 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataaatt atgtcggaaa
    19681 cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt cagacggcat
    19741 tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc cgatgtacct
    19801 accctctatg aagcattccc tgccgctgct ggcgacccta gtgcttgccg cgtgttcttc
    19861 gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaagctg ccgacctttc
    19921 ggcaagcgtt cccacccgcc ctgtcgaacc cgaaggaaaa acgctggcag attacggcgg
    19981 ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg ccgcctattt
    20041 agaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc tgaagtcttt
    20101 gggcgcgcgc agacagtgga cgctgtttgc acaggaatat gccaaactca aacctgaagg
    20161 gcgcgcccaa gaagtcgtgt gttatgccga cttgaaacac agcgactata cgcgtgccgc
    20221 cgaactggtt aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt tggaacaggc
    20281 agccgcatcc ggcttgttgg acagcaacaa tgcctggagg cgcgttcgcg gactgttggc
    20341 caacagccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc cgtttgacgg
    20401 cagcacacaa ggttcgcgcg aatacgccct gttgaacgtc atcggcaaag aggcgcgtaa
    20461 atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc ccgaacaacg
    20521 cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg tgcctgccgc
    20581 cttggactat tacggcaaag tttccgaccg ccgccaactg accgacgacc aaatcgagtg
    20641 gtacgcccgc gccgccctgc gcgcccgacg ttgggacgag ctggcttccg ttatctcgca
    20701 tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac gcagccatgc
    20761 tgcgctgggc aacttgcagc aggcagcaaa actctatgaa caggcggcag cgacgggcag
    20821 gaatttttat gccgtactgg caggggaaga gttgggtcgg aaaatcgata cgcgcaacaa
    20881 tgtgcccgat gccgacacaa acagcgtccg ccgcatggcg gaagacggtg cagtcaaacg
    20941 cgcactggta ctgttccaaa acagccaatc tgccggtgat gcaaaaatgc gccgtcaggc
    21001 tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc tgaccgccgc
    21061 gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg aacgcaccga
    21121 ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg taatccgcca
    21181 cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc aggaaagccg
    21241 cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg ttatgcctgc
    21301 caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaatttt acaccgccga
    21361 cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc tgcaaaacaa
    21421 cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc gatggcaggc
    21481 ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg aaacgcgcga
    21541 ctatgtcaaa aaagtgatga ccaatgccgc ctactacgcc gccctcttcg gcgcgccgca
    21601 catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg atgccgtctg
    21661 aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg gcaaaccata
    21721 aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga cacggcacaa
    21781 gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc gcaaggcaca
    21841 gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat gatgggcaga
    21901 tgcaacaaca gaatttatta aaggaaataa aatgcctgca atccgcgtaa aagaaaacga
    21961 accctttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg gcctgctgac
    22021 cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac gcaaaaaagc
    22081 ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc ccaaaatgta
    22141 ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca ccgcaagacc
    22201 tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc catccgacat
    22261 cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa aaccgcgatg
    22321 cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc cgtcaaacag
    22381 tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat cctgaccaaa
    22441 atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg tcaggacttg
    22501 gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca aatgctctcc
    22561 gccggggaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc ggcaggtatg
    22621 gcggatatgg gcaaagtgat gggcgtattg aaaacccgcc tcgccggcaa agccgatatg
    22681 ggcgaagtca acaaaattct gaaagccgta ctgaccgcct gattgtatga atataaggaa
    22741 aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatgtc ccaatatcga
    22801 atcggcaggg gcaacacggt ttcgatacac cgaaacgggt tttgccgata aacggattcc
    22861 gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga cggcatagat
    22921 ttatttgacc aatttcaagc cttttttggc gggccggggc gcggtttcgg cagaggtgtt
    22981 ttcaggcggc gtgtcggggc gatacgcttc caattcaaac cccatacctt cgccggtctc
    23041 ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg cctgtccgct
    23101 gaaacgggcg gaaaaactga tccaatcgtt gtcgatttga aggttttgcg tggcggtcgc
    23161 gccgatgttg agcataattt cgttgtcgcg gacgtactgc atggggacgc gcgtgtgttc
    23221 gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt cgcagagggc
    23281 gcggaggatg tagggtttgg tggaagtggg cataatggtt ccgtgttgta cgccaaaata
    23341 ggaaaatgcc tgcaaaacgg cgggttttgc aagcatttcg gacttatttg cgcatggctt
    23401 tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg gaagatgcgc tcggcgtatt
    23461 tgagcagctg cgcggcactt ttgcccagtt tgacatcgta gtggtcgagc cgccacagca
    23521 gcggagcaag ggcgacatca atcatagaaa aatcttcgcc gaggatgtat ttgcttttgc
    23581 tgaacgaagg ggcgagcatg gtcagaccgt tgccgatggc ttcgcgcgct tttgcctgtt
    23641 ccttgttggt ggcggcgggg ttttctaaca cttggacgtg gttgaacaat tctttttcca
    23701 tacggtacag caccagccgg ccccggccgc gcataacggg atcgccgggc ataagctgcg
    23761 gatgagggaa gcgttcgtcg atgtattcgt tgatgatgtt ggactcgtgc agcaccaaat
    23821 cgcgctcgac cagcacggga acttggttat acggattcat gacggcgagg tcttcgggtt
    23881 tgttgtaaat atcgacgtct ttgatttcaa aatccatacc tttttcgtac aaaacgaagc
    23941 ggcagcggtg gctgaagggg caggtaatgc cggaatagag ggtcatcata ataattgtcg
    24001 ctcctgtgtg atgcctgcaa aacggctgat ttataaaggt ttaatcgcgc aattatacgc
    24061 gatttccggc acttaatcca gaaattcggc tcaatctgtt gtttttaata tattttcccc
    24121 gattttccgt atcagtgcga acttactgtc tttgttgcgc gggcgcgcac cctgccagac
    24181 cgtctgccag ccttgcgacg catccgcatt ttggggcaag cggacgatac ggtagcggca
    24241 ttgtacatcg ccgacgcggt gcggcaatgt gccgtactgc gtccaaacaa tccgcgtgtg
    24301 caggtcgccg ccgcctatgt cgatacactc gatgccgtct gaaagctccc gttttaattc
    24361 cggggaaagc gatgcctcca tactccggac gaccggcgcg tggcttttcg ccgcgtccag
    24421 ccacggcagg aacagcgtca tcagcaaagc ccaggtcagg gtaacgcctg ccgcccagtt
    24481 ggtaaccgcc tgcctgccgc gtatgttttt ccgggtaatc gcccacagcc acaagggtgt
    24541 gaacagtacg gcaaccgcca tcggaatggg atcgatatca ggaacataat acgggctgaa
    24601 gtaggcggcg cgttcggcaa gcttggcggg ccagccgtaa ttcatagcga aaaaacccgt
    24661 ccacaggaac acggcaaaca gtccgaacgc cataatgccg aaccagttga caaacgccgc
    24721 cgcgccgcgc ctcaggctgt ccagttgcgc cgcgccgaac agggcaagcg gcggcagcag
    24781 ccagacgagg ttgtcctgaa aacgctgcgg attggcggcg agcagcacca aaacggcaag
    24841 catccacaca acgcccaaaa tcccccagtc ggtcgaaaac aggcgcgtgc ggcaaaccgt
    24901 ccaaaccgcc agcggcagcg cgggcagtgc aaaccaaagc aggtttttca gatagtaaaa
    24961 caaactgaat gccgtctgaa cgtgccgcac gccgccgaac gtaccgaaaa cgtgatcgtc
    25021 gagccattgc gcgaacagcg cgggctgcgt ttttgccaag agcagcgggt aaacggtcat
    25081 cagcggcagg gcaaaggcaa gcgaggcgac tgccgtcaac atcaaacgcc tgctttgcca
    25141 cggacggaaa aacatcagca ctggcagggg cagcatcagg gcaaatgctg ccggataagc
    25201 tgctgccaac gacatcagcg tccaacccgt accgagcaga aaagaggcgg caatcacacg
    25261 gcggcacgcc agcgagtagc cgtgcagcac cagtccggcg gcggcaaagg cggcggcagc
    25321 gggattgaga aagtgtacgg tcggaatcag cccgatacag ccgataagga ttaaaacaac
    25381 gctgcgcccg tggtgtctgc ccaaaaagtt gaaaccggca aagccgcagg aagtcagtcc
    25441 gacaacggcg aaaaacacgc ctgcaaagcg tgcggcatcg tatgagtcgg cagcccacgg
    25501 cgacagcaaa tgtttgaacg cggcggcaac ccaaagatac acgggcggta tgccgaaatc
    25561 ggtttgaccg aacagatggg caaccaaggg ggtggggctg cctgccagtg cttcgacggc
    25621 ggtatagacg gcaggttcgg caggattcca taaatcatgt gaaaatacac ccggccacaa
    25681 ccaggcaaac gccatcaaca gcagcagcca cggcttttcg tgggttttgg cgggcgggcg
    25741 ggcatcgggc ggggtatagg tcagcataag cgtgagaaaa aatggacgga ttgccaagtg
    25801 tagcaaatat tcgcacaaag gtcgtgcaga gactgcttca gacggcatca gacacaaaaa
    25861 gaccggcaac aaaaaagact gcacatggca gtctttgcag atactatctt tttcataata
    25921 ttttttccta gcccaacaca gtccatagca tatcaaaact gttgttttca accaggatat
    25981 atatccccaa tcctaaataa acaacagcaa caaaccatct gctatatttt tccaaagttt
    26041 ctccaacaga agggacttgt gctaattttt gggcagaaaa aaccaagaga taaatcatga
    26101 ctagaaaggt aagtaaagcc actatcaaat tcgctaaatt taaggtagta aaatatggga
    26161 caaagacacc aatattgtca gcaccacaac ttgcaaaagt aatcatagcg actagaaaaa
    26221 tcaggttttt attatctttg cgcaaaccct ctttggcaat agcctctcca tcagaatctc
    26281 ctaacagcaa aactttgatg cctaggaaaa ttggaatcaa gccgagcaaa cctaaaatct
    26341 ctttactagg aatataatct aagacaaatg caaaaagcaa acttagcaat atcaggctaa
    26401 cagagcctag aaattgtcct aaatagatgt taatgatgtc ttttctgctt tttcttttgg
    26461 caaaaaataa cattaggata ataagtaagt ctacggctgt cccagaatac aggattattg
    26521 aagtaacaac attttgaatc ataaaacatc tcattcaaat atatttttaa atgtattcaa
    26581 acattaaacc ttgtagatgt caacttcaac cccgtcaaa
//
LOCUS       DAWFKN010000019        26463 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFKN010000019 DAWFKN010000000
VERSION     DAWFKN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.019"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(242..844)
                     /locus_tag="WGR46_000827"
     CDS             complement(242..844)
                     /locus_tag="WGR46_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0855806.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGR46_000828"
     CDS             complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGR46_000828"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0855807.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            2307..2624
                     /locus_tag="WGR46_000829"
     CDS             2307..2624
                     /locus_tag="WGR46_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0855808.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(2676..3287)
                     /locus_tag="WGR46_000830"
     CDS             complement(2676..3287)
                     /locus_tag="WGR46_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0855809.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIVFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(3350..4285)
                     /locus_tag="WGR46_000831"
     CDS             complement(3350..4285)
                     /locus_tag="WGR46_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0855810.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEAIPAQNLSRFVNRKLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(4296..5045)
                     /locus_tag="WGR46_000832"
     CDS             complement(4296..5045)
                     /locus_tag="WGR46_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0855811.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGR46_000833"
     CDS             complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGR46_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0855812.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            complement(6277..6912)
                     /locus_tag="WGR46_000834"
     CDS             complement(6277..6912)
                     /locus_tag="WGR46_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0855813.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            7027..7707
                     /gene="tsaA"
                     /locus_tag="WGR46_000835"
     CDS             7027..7707
                     /gene="tsaA"
                     /locus_tag="WGR46_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0855814.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            7776..7895
                     /locus_tag="WGR46_000836"
                     /pseudo
     CDS             7776..7895
                     /locus_tag="WGR46_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGR46_000837"
     CDS             complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGR46_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0855815.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            9411..12005
                     /gene="mutS"
                     /locus_tag="WGR46_000838"
     CDS             9411..12005
                     /gene="mutS"
                     /locus_tag="WGR46_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0855816.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTTLPQLQKAAKAAAALDVLSTFSALAKERNFARPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPFDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(12129..12620)
                     /locus_tag="WGR46_000839"
     CDS             complement(12129..12620)
                     /locus_tag="WGR46_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0855817.1"
                     /translation="MNSLFVDNTVFITRLKAVHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDGELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGQHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(12617..13018)
                     /locus_tag="WGR46_000840"
     CDS             complement(12617..13018)
                     /locus_tag="WGR46_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855818.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGERRRRGIDR"
     gene            complement(13039..14433)
                     /locus_tag="WGR46_000841"
     CDS             complement(13039..14433)
                     /locus_tag="WGR46_000841"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0855819.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(14549..15205)
                     /locus_tag="WGR46_000842"
     CDS             complement(14549..15205)
                     /locus_tag="WGR46_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0855820.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGR46_000843"
     CDS             complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGR46_000843"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0855821.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            15679..16161
                     /locus_tag="WGR46_000844"
     CDS             15679..16161
                     /locus_tag="WGR46_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0855822.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            16169..16360
                     /locus_tag="WGR46_000845"
     CDS             16169..16360
                     /locus_tag="WGR46_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855823.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            16357..17007
                     /gene="ftsE"
                     /locus_tag="WGR46_000846"
     CDS             16357..17007
                     /gene="ftsE"
                     /locus_tag="WGR46_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0855824.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            17004..17921
                     /gene="ftsX"
                     /locus_tag="WGR46_000847"
     CDS             17004..17921
                     /gene="ftsX"
                     /locus_tag="WGR46_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0855825.1"
                     /translation="MSIIHYASLHIESARTALKQLLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDDNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            18022..18270
                     /locus_tag="WGR46_000848"
     CDS             18022..18270
                     /locus_tag="WGR46_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0855826.1"
                     /translation="MLTPEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(18338..19516)
                     /locus_tag="WGR46_000849"
     CDS             complement(18338..19516)
                     /locus_tag="WGR46_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0855827.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMADKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(19597..20850)
                     /gene="murA"
                     /locus_tag="WGR46_000850"
     CDS             complement(19597..20850)
                     /gene="murA"
                     /locus_tag="WGR46_000850"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0855828.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            complement(21070..21145)
                     /locus_tag="WGR46_000851"
     tRNA            complement(21070..21145)
                     /locus_tag="WGR46_000851"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21110..21112),aa:Lys,seq:ttt)
     gene            complement(21214..22188)
                     /locus_tag="WGR46_000852"
     CDS             complement(21214..22188)
                     /locus_tag="WGR46_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0855829.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            complement(22221..22670)
                     /locus_tag="WGR46_000853"
     CDS             complement(22221..22670)
                     /locus_tag="WGR46_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0855830.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            complement(22711..23982)
                     /gene="waaA"
                     /locus_tag="WGR46_000854"
     CDS             complement(22711..23982)
                     /gene="waaA"
                     /locus_tag="WGR46_000854"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0855831.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHCPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLS
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(24045..25493)
                     /gene="gnd"
                     /locus_tag="WGR46_000855"
     CDS             complement(24045..25493)
                     /gene="gnd"
                     /locus_tag="WGR46_000855"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0855832.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FTAWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGTDPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            complement(25573..25994)
                     /locus_tag="WGR46_000856"
                     /pseudo
     CDS             complement(25573..25994)
                     /locus_tag="WGR46_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6605 a         7432 c         6066 g         6360 t
ORIGIN      
        1 aatcatcttc ggcgttttca tttttatgga ttaaaacaac acgggaaaaa tctgttttca
       61 gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg tcctcaaaat
      121 taaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca tgtagagaaa
      181 tgctatccga aaaatttttt ctttgtgtat gcaaaaaaag ttttcattca agtacccata
      241 tctaacgcaa acgtttacct gtttccccgt caataatctg actcggcgat ttctgcctgc
      301 cgattctccc accaacaatc cacacatcgc gtccgaattg ccttctgact tccctctccg
      361 tccgacacgc gcgtttgcct gcgcggttgc acgaagtcga gaccaaaggc gtttgcaaag
      421 cctgacacaa gcggcgcgca cctacatggg cgggaaccct gaccgccaac ttgctgcgct
      481 gtttcccgcg caactcaggt aagacacagg atttggcgga taataggaac gttttagggg
      541 cgggccattc ttttctaagc atatcctgca gattttcaga cggcatttga agtaaaggct
      601 gcaattgctc aaattgattc ccgatgacaa tcataccctt gtgttgcggt ctttttttca
      661 aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag ccataacaag
      721 attcggtcgg ataagcgacc aaaccgcctt ttttcaaata aacgcttaac ttacgttgcg
      781 ctgatgctgc gataattctc ggaaataaca taatataaaa taccgtctga agcacatcag
      841 tcatacttgg cttcagacgg catcatcctc tttctaatta acggttaatc gctttatcgg
      901 caatgtcttt acggtattgc atcccgtcga aactgatttt ttccaacgcg ccatatgcct
      961 tagctttcgc ttgcgccaca ttatcgccca atcccacaac acacaatacg cgtccgccgt
     1021 tggtcaatac gtcacctttc tcgtttgccg ttgtacctgc atggaaaact ttgcctactt
     1081 ggttggcagc atccagaccg gaaataacat cgcctttttt gggcgtttcg gggtaatttt
     1141 gcgccgccag taccacgccc acggcagttt gcgggctcca ttccgcggtt acgctatcga
     1201 gtttgccgtc tattgccgct tcaaccaaat ccgataagtc gctgttcagt cggctcataa
     1261 tcggctgggt ttcaggatcg ccgaaacggc agttaaactc aatcgtatgg ggtgcaccgc
     1321 tttgatcaat catcaaacct gcgtacagga aaccggtgaa ctcatgcccc tccgctttca
     1381 tccctgctac ggtcggcaaa ataatttcat tcatcgcgcg ttcgtacaca acaggcgtta
     1441 ccacaggcgc agggctgtac gcacccatac cgcccgtatt cagacctttg tcgccgtcta
     1501 aaagacgctt gtggtcttgg ctggttgcca taggcagtac attattgcca tcaaccatga
     1561 cgataaaact cgcttcttcg ccttgcagga aatcttcaat cacaatacgc gcgccggcat
     1621 tgcccatttt gttgtccagc agcatatcat caatcgcagc atgcgcttca tccaaagtca
     1681 tcgccacaat cacgccttta cctgccgcca aaccatcggc tttgataacg ataggcgcac
     1741 ctttctgatt gacgtaatca tgtgcggcat cggcgttttc aaaggtttga tattgcgcgg
     1801 tcggaatatt gtatttcgcc ataaatgctt tggcgaaatc tttggaactt tccaactgcg
     1861 ccgcatattg tgtcggaccg aatattttta gtcctgcagc acggaaatca tccacaacac
     1921 ctgccgccaa aggcgcttca gggccgacga cggtaaaaac aatattttct ttacgacaga
     1981 attcaatcaa atcctgatgc gcagtcaagt cgatgttttg cagcttgggt tcaatcgctg
     2041 taccggcatt accagacgca acaaatactg tttccacttt aggcgactgc gccaatttcc
     2101 aagccagcgc gtgttcgcga ccgccattac cgataaccag cagtttcatg ccatctcctt
     2161 gacaaatatg tacttttaac gaaaactcga tacaaaggga cttttatccc atctgaagaa
     2221 attttagtag aatcaaacaa aagaccgctt cattccactc tgcaacctat tcaacttatt
     2281 cataaattaa aaaaggacaa gcaaccatgc aaaaacgtat tgatgaaatc caaagcaaat
     2341 accgcgaatg gtgtcattta ctaccgcaat tggaagaaga catccgccgt tggaaacatg
     2401 ccgtcgcttt gattcgcgat atggacaatt tttataccca cgaatatcag gcttgtcatc
     2461 gggctattga agatggagcg gaggtggatt tgcgcacaga aggcgaatac agcattatga
     2521 gtgaagatgc actatggaac gcgctgggcg aattccatca actggcttgg ttatatttgc
     2581 gctccagcgt cgatgcctta gacaaatata cacaagaaga ttagtcagcg aagaggtcgt
     2641 ctgaaatacc atcacaaagc atttcagacg acctttcatt caaaaggctt ttccgtattt
     2701 acttcaatct gccgtgtact cttccaagcc gcaacacagg cctcataatt taccaacgac
     2761 aaactgaccg tcaatcggca atccaactgc aaatcccgct ccaatacatc cgcctgatat
     2821 tgtttagcaa tgcgtatcgc ttcattcaaa aacggatatt cacatttcag ccaaacagtt
     2881 ttttcaatat tcttttcaac tacttctgca actgccaacg cttgagccgt cgcctctttg
     2941 tacgcatgta tcagacctgg aacacctaac aaagtaccac cgaaatagcg gacgaccacg
     3001 accaaaacgt cggtaatacc caccgaatca atctgtccca aaattggtcg tccagcactt
     3061 cctgatggct ctccatcatc gttggcacga aattgcacac catccacacc caaacgatag
     3121 gcatagcacc agtgtcgtgc tttatgatgc tcttccttta acggatcgag gtattttttc
     3181 acatcagcca atgtccgaat cggataggca aatacaataa aacggctgcc tttatcttta
     3241 aactcagcct gcgtcgagga ggtaatggtt ttataagtcg taatcatgct gaaatgtttt
     3301 cagacgacct cattaataac aaggtcgtct gaacgtttca cgtgaaacat taatttttca
     3361 atacttctgt taattgtgga acgatttcaa ataaatcgcc aaccaatccg taatcggcta
     3421 cattgaaaat cggcgcatca gcatctttat tgattgcaac aatcacctta ctgtcttgca
     3481 taccggcaac gtgttgaatc gcacctgaaa taccgattgc aaaatagagt tgcggcgcaa
     3541 ctactttacc ggtttgtccg acttgagcat cgtttggcgc atattcggca tcaaccgccg
     3601 cacgggatgc accgattgcc gcacctaaaa catccgccaa cggtgtcagc acttcattga
     3661 atttttccgc actacccaac gcacgaccac cggaaacaat cacttttgcc tgagtcagtt
     3721 caggacgatc ggaatgagaa agcttgcggt tcacaaaacg actcaggttt tgagcaggga
     3781 ttgcttcaac attaattacc tcggcatgac caccttgtac aggagctgca tcaaaagccg
     3841 tcgcacggaa ggtcagcacc aatttttccg aatcaacttg cacggtttca aatgcgttcc
     3901 ctgcataaat ggggcgcaca aaaatcgtgt tatccacaat ctcggtcaaa tcagaaattt
     3961 gcggtacatc taataaggct gctacgcgag gcaaaaggtt tttaccgaat gtagttgccg
     4021 ttgctgcaac atagcggtaa tccgctgcca atttaacaac cagcggagcc aactcttcag
     4081 ccaaaccttc ggcataatgg gcagcatctg caaccaaaac tttttccaca cccgccactt
     4141 gttttgcgga ctctactacg gcagatgcac cgcttccggc aaccaataaa tcgactttac
     4201 ccagcttggc ggcagcggta acagcatgca aagtggtagg attcaactgt ttgttgtcgt
     4261 gttcgacaat aatcaatacg ctcatttcag cctcctcaaa tcactttggc ttcgtttttc
     4321 aatttttcaa ccaattcggc aacgcttgct acttttacgc ctgcctgacg cgccttaggt
     4381 tcggcaaatt tcaccgtttt cagacgaggt aaaatatcgg tagccaaatc ggcaggcgcc
     4441 agtttttcca gaggtttttt ctttgccgcc ataatattgg ggagtttaac aaagcgcggc
     4501 tcgttcaaac gcaaatcagc actgataaca gcaggcagtt ttaatgctat ggtttcttcg
     4561 ccgccatcaa tttcgcgcac aatctgtact tcaccacctt caatgtgtac ttttgaagca
     4621 aacgtacctt gcgccgcatt cagcaaagct gccagcattt gcgccacttg attggcatca
     4681 tcatcaatcg cttgtttgcc caaaaagaaa atttgcggat tttctttgtc cgcaacggct
     4741 ttcagcaact tagcaacggc cagagactcc agtttagtat cggtttcaac atgaatggca
     4801 cggtcggcac ccatcgccaa agctgtacgc aaggtttctt cgcatttttt ctcacccaaa
     4861 gaaaccgcta cgatttcgct tacttttccg gcttctttca aacggacagc ttcttccaca
     4921 gcgatttcgt caaacggatt catcgacatt ttgacattgc cgatatccac atccgaacca
     4981 tcggctttta cacgaacttt gacgttgtag tccactacgc gctttactgc gaccagtgct
     5041 ttcattgaac cctcctaaaa agaacgctgc tttcaccatc cagcgaaacc aaaccttctt
     5101 ccctataaaa ccaaatccgt tttccttaaa aacgaattca ttcaaaaatc tttcggataa
     5161 tgcttgccga ttataccatt tttaaagcat ttactcagac tagcggatat acattcctgt
     5221 atctaataaa ttggaaaata tcatgccgcc atatcagttt tagacgaccc tttagccttt
     5281 atctgctgca acacaatcca tcagcgcttg ataaaccaaa tctgcggtcg gaatctgccc
     5341 gatattgccc aaattttttg caattggcga aacctgaacg cctgttttaa tcggatcggt
     5401 atcggtataa atgccgacca caggtttttc caaggcgttt gccaaatgca gcaaaccggt
     5461 atccacgcca acaattccga ccgcgtgttt cagcagatac gctgcctgca ataaattcat
     5521 tttatcgcac acaatagcaa acggcagtcc gtctgcaatt tgtttggcac gcgttttttc
     5581 atcttcattt ccccaaggca ggtaaatatt gcattgctgt tcttcattca acttttgcag
     5641 caacgaccgc cagttttcca caggccataa cttactgtcc cgactggtcg catgcaaagc
     5701 cgcataatac ggctgcgcta aatttttcag acggcctgcc tcaggaacag tcaagccaaa
     5761 tacctgcgtt tcaggcatcg catacccaaa tacttgggcg aacagctcac gattgcgcca
     5821 aacagcattt ttccctttcg gtacagcgta tgttttcgca tacgccagcg cagccagtcc
     5881 ctcgcgcgcg ctatttttat ccaaaccata aataggggat ttcgccattt tagcgaaaca
     5941 cgcgctttta atcagacctt gactgtccaa tacaaaatta aatgcttcct gccgcaaagt
     6001 ctgtttcaga tgacccattt cccgccaagt ttcagcctga aagagatgtt tgcgccattg
     6061 ccgccatttc atcacatgga ttttttttac aaacggatgc aggcgcgcaa tatctgcaaa
     6121 tccagcctca catagccaat gcagttctac atcaggacat tgtcgcgcca aatcttcgat
     6181 tgcgggcaaa gtgtgaatta aatcgcccat actagacaag cggacaagca aaattttcat
     6241 atttaagaag tatgtttcac gtgaaacgat tttaacttat tgattattaa tatatttttt
     6301 aatttcttcg gcgtttttta agatgacagc accggcagaa cgcatttcct gccatgccgt
     6361 ttcaatggta tccggcgcaa taccccgaca agccgcttca ttgacgataa cctgccaatt
     6421 gctacctttg agcaactgca aaaccgttgt tttaacacaa taatccgtag ccaaaccgcc
     6481 gacaatgacc atatccgtat tttgacaacg cagccactca atcaatccag tgctcagttt
     6541 ttcctcaata tcgtgaaaac acgcgccgta aggatgcaat tcaggatcaa cacctttcca
     6601 aacgcaataa tcgtattctt tggcagaagg cagtccgtcc aataattcat agccgcgcgt
     6661 accgaccatc gcatgagcca cccaagtcaa atccgcatca ggcaaacctg tcggcttcaa
     6721 catatcaaca gggttatcca caagccattt cgctaccata tgatgcgcat ctttcgtcat
     6781 cacgcgcaaa tccgccaaag cggcttgggc attcaattct tcgacaatca aatgcccctc
     6841 gtttacaggc agctctttag gacacaaagg cgtaaaagtt ttttgtgcat caacatcaat
     6901 ggaaacaatc atctcatcat ttcaacgcaa ttaaaatgcc ctgtattata acaaattact
     6961 gcccaaaagc ggtaaaacag gctgtgataa gataaggttt ttccgaaaaa cttatccaca
     7021 atcctcatga cttacaccat cgtccctatc gccaccgccc gatcgcccta caaacagaaa
     7081 ttcggcatcg cccgccagcc cggtttagtc tccgccgcag aagtatgcat cgagctgaat
     7141 cccgaattta ccgcagacag cgtgcgcggg ctggaagatt tcgattatgt gtggataagt
     7201 tttatttttc acggcgtatt ggatgaaggc tgggcacaaa tggtgcgccc gccacggctc
     7261 ggcggcaaac aaaaaatggg cgtattcgcc acgcgcagcc cccaccgccc caaccatctc
     7321 ggactatcac tcttaaaact caaccgcatc gaaaccggca aacccgtccg cctttattgc
     7381 agcggcgcag acctgctcga cggcacgccg attgtggaca tcaagcctta tatccccttt
     7441 gtcgaatcca aacccgatgc cgcatctggt ttcgtcagcg gcaaacccgt agagttggaa
     7501 gtcgtttggc aggaaaacat cggcgcggaa aatttatctg caaacaccaa aaaccttatc
     7561 agccaaagca ttgcccaaga tccgcgcccc gcctatcaga atattcccga acggatttat
     7621 gtgatgaata ttgcggatta cgaaatcaga tttcaaatcg aggaaaaccg tgcaaccgtt
     7681 attgatcttt ccccaacccc gctttaaatc gggcaaaaat ccggttttgc cgcatagcag
     7741 ttgaacaaac ggctgttgtt tgttcgccat aagccgcaat atcaagttat agcggattaa
     7801 atttaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7861 taacgccgta ctggtttaaa tttaatccac tatacagata aacaatgccg tctgaacgca
     7921 atgtgttcag acggcattta cttatccaca ggtttgttca agccttagat tttgcctgcg
     7981 aagtattcca aagtgcggac gagttggcag gtgtaggaca tttcgttgtc gtaccaggca
     8041 acggttttca ccagttgttt gccgcccacg gtcatcacgc gggtttgggt tgcgtcaaag
     8101 agcgagccgt attcgatgcc gacaacgtcg gaagaaacga tttgatcttc gttgtagccg
     8161 taagattcgc tggcggcggc tttcatcgcg gcgttgattt cttctttggt tacagggcgt
     8221 tcgaggacgg aaaccaattc ggtcagcgag ccggtggcaa cagggacgcg ttgggcggag
     8281 ccgtcgagtt tgccgttcaa ttcggggata accagaccga tggccttggc ggcaccggtg
     8341 ctgttgggca cgatgttgag cgcggcggct cgggcgcggc gcaaatcgcc tttgcggtgc
     8401 ggcgcgtcaa gggtgttttg gtcgccggtg taggcgtgga tggtggtcat cagaccttcg
     8461 actacgccga actctttttg caggactgcc gccatcgggg caaggcagtt ggtggtgcag
     8521 gaagcggcgg aaatgacggt ttcgctgccg tccaaaatgt cttggtttac gccatatacg
     8581 acggttttca catcattgcc gccaggtgcg gaaatcacaa ctttgcgcgc gccggcacgg
     8641 atgtgggctt cggctttggt tttgttggta aagaagccgg tacattcgag gacgacatcc
     8701 acacccaact cgccccaagg caattcttcg ggattcggat tggcaaaaac tttgatttct
     8761 ttgccgttta ccacgatggc atcgtctttt aattcggcag taccttggaa acggccttgc
     8821 gtgctgtcgt atttgaaaag gtgcagcagc atttcggcag gggtcaggtc gttgacggcg
     8881 acgacttcga tgtcgtgggc tttttcaatt tgacgcaatg cgaggcggcc gatgcggccg
     8941 aaaccgttaa tcgctacttt aatgctcata ttttgtctcc aggttgtgaa ataaagtccg
     9001 atgcctgcat tgtattctga aataaagtta cattccacta ttacatctaa ctacttgacg
     9061 cttatttgat atagatgaat tttgctgttt gcacagattt ccaaaacctt taccatcaat
     9121 atttgaattt aaattttaat gatgattttg atgattgcca acctgcttgt gcgtaagtag
     9181 caaatatcca atattttcat tatctttttg tcaaataagt ttgagtttaa gacttgctgt
     9241 ataagacaga taagcgtgga tattttttga cttaataata tttctgtgga taactttgct
     9301 gttttcctag ttgtctccac aaccttattg acaggcttac ggtcagtctc attccgtcga
     9361 agacaaaacc ttttgctaca ataccgtttt cctaatgata aggcagcccc atgtccaaat
     9421 ccgccgtttc cccaatgatg cagcaatacc tcggcatcaa agcgcaacat accgacaaac
     9481 tggtgtttta ccgtatgggc gatttttacg agatgttttt cgacgatgcg gtagaagcgg
     9541 caaaactttt ggatattacc ctgaccacgc gcggacaggt ggatggcgag ccggtcaaaa
     9601 tggcaggcgt gccgtttcac gccgccgaac aatatctggc gcgcctggtc aagttgggca
     9661 aaagcgtggc gatttgcgaa caggtcggcg aagtcggcgc gggcaaaggg cctgtggagc
     9721 gcaaagtcgt gcgcatcgta acgcccggca cgctgaccga ttccgcattg ctggaagaca
     9781 aggaaaccaa ccgtatcgtt gccgtgtccc ccgacaaaaa atacatcggt ttggcgtggg
     9841 catcgctgca aagcggcgaa ttcaaaacca agctgacaac tgtggataaa ttggacgacg
     9901 aactggcgcg cctgcaggcg gcggaaattc tgttgccgga cagtaaaaac gcaccgcaac
     9961 ttcagacggc atcgggtgtt acgcgcctga acgcgtggca gtttgccgcc gacgcggggg
    10021 aaaaactgct gacggaatat ttcggctgcc aggatttgcg cggcttcggt ttggacggca
    10081 aagaacacgc cgttgcgatt ggcgcggcag gtgcgctgtt gaactatatc cgcctgacgc
    10141 aaaacctgat gccgcaacat ttggacggcc tgtcgctcga aaccgacagc caatatatcg
    10201 gcatggacgc cgccacgcgc cgcaatctcg aaatcacgca aaccctctcc ggcaaaaaat
    10261 cgccgaccct gatgtccacg ctcgaccttt gcgctaccca tatgggcagc cgcctcttgg
    10321 ctctctggct gcaccaccct ttacgcaacc gcgcccacat ccgagcgcgc caagaagccg
    10381 ttgccgcgct ggaaagccaa tacaaacccc tccaatgccg tctgaaaaac attgcggaca
    10441 ttgaacgcat tgctgcccgc atcgccgtcg gcaacgcccg cccgcgcgat ttggcatctt
    10501 tacgcgacag tctgttcgag ttggcacaaa tcgacttgtc cgcaaacggc agcagcctgt
    10561 tggaaaccct caaagccgtt ttcccggaaa acctatccac agccgaacag ctccgccaag
    10621 ccattttgcc cgaaccttcc gtctggctga aagacggcaa tgtcatcaac cacggttttc
    10681 atcccgaact ggacgaattg cgccgcattc aaaaccatgg cgacgaattt ttgctggatt
    10741 tggaagccaa ggaacgcgaa cgtaccggtt tgtccacact caaagtcgag ttcaaccgcg
    10801 ttcacggctt ttacattgaa ttgtccaaaa cccaagccga acaagcacct gccgactacc
    10861 aacgccggca aaccctcaaa aacgccgaac gcttcatcac gccggagttg aaagcctttg
    10921 aagacaaagt gctgactgct caagagcaag ccctcgcctt agaaaaacaa ctctttgacg
    10981 gcgtattgaa aaaccttcag acgacattgc cgcagcttca aaaagccgcc aaagccgccg
    11041 ccgcgctgga cgtattgtcc acattttcag ccttggcaaa agagcggaac ttcgcccgcc
    11101 ccgagtttgc cgactatccg gttatccaca tcgaaaacgg ccgccatccc gttgtcgaac
    11161 agcaggtacg ccacttcacc gccaaccaca ccgaccttga ccacaaacac cgcctcatgc
    11221 tgctcaccgg ccccaatatg ggcggcaaat ccacctacat gcgccaagtc gcgctgattg
    11281 ttttattggc acacaccggc tgttttgtgc ctgccgatgc cgccacaatc gggcccttcg
    11341 atcaaatctt cacccgcatc ggcgcatcgg acgacctcgc ctccaaccgc tccactttca
    11401 tggtcgaaat gagcgaaacc gcctacatcc tgcatcacgc caccgaacaa agccttgttt
    11461 taatggacga agtcggacgt ggtacttcca ctttcgacgg cctcgccctc gcgcacgccg
    11521 ttgctgaaca cctgctgcaa aaaaacaaat ccttcagcct gtttgccacc cactatttcg
    11581 agctgaccta cctgcccgaa gcccacgccg ccgccgtcaa tatgcacctt tccgcgctcg
    11641 aacagggaca ggacatcgtt ttcctgcacc aaatccaacc gggtcccgcc ggtaaaagct
    11701 acggcattgc cgtcgccaaa ctcgccggcc tgcctgtacg cgcattgaaa tccgcccaaa
    11761 agcatttgaa cgaactggaa aaccaagccg ccgcgaaccg tccccaactg gatattttca
    11821 gtaccatgcc gtctgaaaaa ggagatgaac cgaatgtgga cagctttgtg gacaaagcag
    11881 aggaaaaaca ttttgaaggt atattggcag cagccctgga gaaactcgat cccgacagcc
    11941 tgaccccgcg cgaagcattg tcagaactgt accgtctgaa agatttgtgc aaatccgtat
    12001 cttaatttcc gttgtcggaa cagcatcaaa ccatatggaa aaatctgtgg ataaacatta
    12061 tctgacagga aatttccaaa tataaaaaat gccgtccgaa cagctcagac ggcatctgtc
    12121 cattcggctt aaaccttatc cacatccaaa cgcataaccg taacccattc accgttatgg
    12181 aaatgttgcc cgacaaccac ccagccgaat gattcataaa atatttgcac atcaggcgta
    12241 taaagataca agaactttat ccccagcgaa cgcgctgcgc ctatgcagtg ggcgaccagc
    12301 ctcctgccaa tgccttttcc gcgatattca ggtaaaacaa agacatcacc caaccaatat
    12361 tcatactgtg gaaaactttc catatcatgc cgcttgaccg tagccgaacc caacaggatt
    12421 ccggagtcat ccacagccgc aaatgccagt ggcagttcgc catccttcaa acacctgccg
    12481 taataggcat gaatcttatc cacagaagac cacggttcaa atccgtgcca ctcctcaaac
    12541 aacgcctgaa ccaacctgcc gatatgcacg gctttcagcc gtgtaatgaa aacagtattg
    12601 tctacaaaga gggaattcat cggtcaattc cccgacgcct tcgttcccct gcgccgtaaa
    12661 ccgcattcca agcatggtcc aaacgcactc cgatttgcct cagctcttca gcctgccggg
    12721 ctttttgcgc cattgctgca ggaatttccg cttccaaacg ggcgatgtct gcctgagccg
    12781 cctgcaaacg ccggcgcgca tcttccaaat ccgactgcat cccgatgatt tttccgtcca
    12841 gattgttttg cttttgcaat aaggcacggt aaccggattg gatactgagc aaattgtctt
    12901 cagcatcccc tgcccatacg cttgtagaaa aaacaaacat cagaaaatac aatatttttt
    12961 tcatgttcaa cttccgtttg aatgccgtct gaagccgcat tccgacatca gacggcatta
    13021 cccgaacctg tggataactt aagcgcggat gcgtttcaac acttcttctt tgccgattaa
    13081 tgccaacaca gcatcgacgc tgggggtttt cgccgtaccg cagacggcaa ggcgcagggg
    13141 catgccgagt ttgcccattt taatgccttc ttcgtcgcag aagggtttga agaggtcgtg
    13201 gatggcttcg gcattccagt cttccagccc ttcgaggcgt tcggcaaagc gcagcatacg
    13261 ggcggcggct tcatcgtccc agtgtttctg cacgtccgct tcagtaggcg tttgtttgac
    13321 gtagaagtag aggcactcgt cggcaagtgt gttcaggtct tgggcgcggt ctttgaccag
    13381 tgccaacacg tcttccaatg caggtttttc agtttcatgg atatcgcgca gagcgagacg
    13441 cggtttgacg agttcggcga gtttgccgtt gggcgtgatt ttgatgtgtt cgccgttaat
    13501 ccagtagagt ttcttcaaat ccatgcggct tggagatgga gaaacgtctt tcaaatcaaa
    13561 ccattcgata aattgttcca tcgtgaagaa ctcatcgtcg ccgtgcgccc agcccaaacg
    13621 tgccagatag ttgagcatgg cttcgggcag gatgcccatc gcgccgaaat cggtaatggc
    13681 aacggtatcg ccgctgcgtt tggagatttt tttgccttgt tcgttaagaa tcatcggcag
    13741 gtggccgtat tcaggcaggg tagcgccgat ggcttttaag atgttgattt gtttcggggt
    13801 gttgttcaca tggtcgtcgc cccggataac gtgggtaacg cccatgtcgt agtcgtccac
    13861 gacaacgcag aagttgtagg tcggcgtgcc gtcggcgcgg gcgataatca ggtcgtcgag
    13921 tgcttcgttg gggatggaga tttcgccttt gaccaagtcc gtccacttgg ttacgccatc
    13981 caaaggcgtt ttgaagcgga cgacgggttg cacgccggca gggatttcgg gcagggtttt
    14041 gcctgcttcc ggccgccagc ggcggtcgta agtcgccgtg ccttcttttt cggctttctc
    14101 gcgcatcgct tccagctctt ctttgctgca atagcagtag taggcatcgc cttttgccaa
    14161 gagttcggcg atgacttctt tataacggtc gaagcggcgg gtttggtaca ccacgttgtc
    14221 ggcgttgtcg taatcgagac cgacccattc cataccgtca aggatgatat tgacagattc
    14281 ggcggtggaa cgcgccaagt cggtgtcttc gatacgcaat aaaaattcgc ctttatgatg
    14341 gcgggcaaac gcccacgaaa acaaggcggt gcgaacgccg ccgatgtgca ggtagccggt
    14401 agggctgggg gcgaaacggg ttttgacggt catgatggct ccagaatctt taaaacggct
    14461 tattttactg tttttaccgt gcttgggcat taaaaatgcc gtccgaaccc tgcctgcgga
    14521 taagtttcag acggcatttt ctaccgcttc aatcagacag ccatcccaaa aagcggctgc
    14581 cccatttttc caaacggtag agggataacg catatccctc ttgcagcata aagatttttt
    14641 tcttatttcc cgcatcaaag cgcgtggtcg gcgtggcaga catataaacg cggacaccca
    14701 aatcctccgc catttccgcc gcccgcgcca aatggtaggg atcgctgaca atcaccacac
    14761 tggcaatacc gttggcacgc aaaaccggac ggatgttgtt caggttttca taagtgttgc
    14821 gcgaagtgtt ttcaaacagg atgttgcgcg ccggaacccc ctgtttgagc gcgtaccgcc
    14881 gcccgacctc ggcttcggtc atatagcctt ttttggtccg gcctcccgta aacacgattt
    14941 tgcctaccct gcggctttgg taaagcgcga tggcgtggtt gatgcgttcc cgaaaaaccg
    15001 gggaagggcg tttgtcccat gcggcggcac ccaacaccag cgcggcatcc gcccggacat
    15061 acggcggcaa aacctgcccg cccgtccgat aaaccgccca aacggatgag gcaaacacca
    15121 gcaaaagcgg aaaaacactc aaacagaaac cgcccaacag gtaatagcgc aagccgttgc
    15181 ggctgcaaaa cagccgtttt ttcacaatac cgcttcgata ttttccaacg gcctgccgac
    15241 agccgcctta ccgtttgcca aaacaatcgg acgctccaac agggcgggat gatcggcgat
    15301 ggcacgcagc agtgcgttat tgtccaaatc ggggttgtcc aaacccaatt ccttgtacaa
    15361 atcatctttc acacgcatca tcccgcgcgc cgactccaag cccaatttgt tgaaaatatc
    15421 cttcaattcg gacaagtcgg gcggcgtatc caaatatttg accacttcgg cagcaatgcc
    15481 gcgttcttcc aatagggaca aggcggcacg cgatttgctg caacgcggat tgtggaaaat
    15541 tttgatttca gacatgacat ttccttactt ctcgacaatc cccttattat cggcttacac
    15601 agggttttac tcaatatccc gcctacaacc gtaccaaacg gtttacaata cccgaatcga
    15661 catacaaagg acaaaacgat gaaatacttg aatcttgccg caatcaccct tgccgccaca
    15721 tttgccgcac ataccgcctc ggcagacgaa ctggccggat ggaaagacaa caccccgcaa
    15781 agcctgcaat cactcaaagc ccccgtacgc atcgtcaacc tttgggcaac ctggtgcggc
    15841 ccgtgccgaa aagagatgcc tgccatgtcc aaatggtaca aagcgcagaa aaaaggcagt
    15901 gtcgatatgg tcggcatcgc gctcgacaca tccgacaata tcggcaactt cctcaagcaa
    15961 acccctgttt cctacccgat ttggcgttac accggggcga acagccgaaa ctttatgaaa
    16021 tcctacggaa acaatgtcgg cgtactgccc tttaccgtcg tcgaagcccc gaaatgcgga
    16081 tacaaacaaa ccattaccgg ggaagtgaac gaaaaaagcc tgaccgaagc cgtcaaactc
    16141 gcccattcaa aatgccgtta aacgccggat gccgtctgaa tccgcttcag acggcatttt
    16201 tcccgcccga ccttcggtat ccgccaaact tatccactat ctaaaaacag gcggaatctt
    16261 tataatcggc actgtcttac ctattgttca gacggcatat ccctgcggac gcaaccgccc
    16321 gaaacgatat gccgcccatt ccttacagga cctcctatga tccgtttcga acaagtttcc
    16381 aaaacctatc ccggcggttt tgaagccctg aaaaacgtca gcttccaaat caacaaaggc
    16441 gagatgattt ttatcgcagg acactccggt tcaggcaaat ccaccgtcct caagctgatt
    16501 tcgggcatta ccaaaccaag caggggcaaa gtccacttca acaaccaaga cctcggcaca
    16561 ctgaccgaca accaaatcgg ctttatgcgc caacacatcg gcatcgtgtt ccaagaccac
    16621 aaaatcctct acgaccgcaa cgtcctgcaa aacgtcatcc tgccgcttcg gattatcggc
    16681 tatccgccgc gcaaagccga agaacgcgcc cgcatcgcca tcgaaaaagt cggcctgaaa
    16741 ggacgagaat tggacgatcc cgtaaccctc tccggcggtg aacaacaacg cctgtgcatc
    16801 gcccgcgccg tcgttcacca gcccggcctg ctgattgccg acgaaccctc cgccaacctc
    16861 gaccgcgcct acgcgctcga catcatggaa ttgttcaaaa ccttccacga agcgggaaca
    16921 accgtcatcg ttgccgccca tgacgaaacc ctgatggcgg actacggaca ccgcatcctg
    16981 cgcctctcga aaggacgact cgcatgagca tcatccacta cgcctcgctg cacatcgaat
    17041 ccgcccgcac cgcgctcaaa cagcttctgc gccaaccctt cggcacgctg ctcaccctcc
    17101 tgatgctcgc cgtcgcgatg accctgccgc tgtttatgta tttgggcatt caaagcgggc
    17161 aaagcgtgtt gggcaaactc aatgaatcgc cgcaaatcac cgtctatatg gaaaccgccg
    17221 ccgcacaaag cgacagcgat accgtccgca gcctgctggc gcgcgacaaa cggctcgaca
    17281 acatccgctt catcggcaaa gaagacggtt tggaagaatt acagtccaat ctcgaccaaa
    17341 atctgatttc catgcttgac gacaaccccc tgccggatgt ctttatcgtt acccccgacc
    17401 cggcaaccac gcccgcccaa atgcaggcaa tctaccgaga cattaccaaa ctgcctatgg
    17461 tcgaatccgc caacatggat acggaatggg tacagacact gtaccaaatc aacgaattca
    17521 tccgtaaaat cttatggttt ctctccctga cgctgggtat ggcgttcgtc cttgtcgcgc
    17581 acaacaccat ccgcctacaa atcctcagcc gaaaagaaga aatcgaaatc accaaactct
    17641 tgggcgcgcc cgcgtcgttt atccgccgcc cattccttta tcaagccatg tggcagagca
    17701 tcctttccgc cgccgtcagc ttggggcttt gcggttggct gctctccgcc gtgcgcccat
    17761 tggtcgatgc cattttcaaa ccctacggct tgaacatcgg ctggcgtttc ttctacccgg
    17821 gagaaatcgt gctggtgttc ggcttcgtca tcgcgttggg cgtattcggc gcgtggcttg
    17881 ccaccaccca gcacctgctc ggcttcaaag ccaaaaaata aaaaaaaata aaacaccgtc
    17941 aaaaatgccg tccgaacccg ttttcagacg gcatttcaat ttgccagtat aatggcgcat
    18001 ttttccaaca aggaacctac catgctgacc ccggaacaag taaaagccat gattgaaggc
    18061 gtggcaaaat gcgaacatat cgaagtagaa ggcgacggac accatttttt cgccgtcatc
    18121 gtttcatcag aatttgaagg caaggcacgc cttgcacgcc accgcctgat taaagacggg
    18181 cttaaagccc aactggaaag caacgaactg cacgcacttt ccatttcggt agccgccaca
    18241 ccggcagaat ggaatgccaa agcacaataa ccgccacaca aaaatgccgt ctgaaacagc
    18301 acaacgtttc agacggcatt tttttatatc aaaccgttta ctcgccgcgt ttttccaaag
    18361 cggctacggc aggcaactct ttaccttcca agaactcaag gaacgcgccg ccgccggtgg
    18421 agatgtagcc gatttggtcg gtaacgccga atttggcaat cgccgccagc gtgtcgccgc
    18481 cgcccgcaat cgagaatgct ttgctttggg cgatggcttc ggcaagggct ttcgtaccgc
    18541 ctgcgaactg gtcaaactca aacacgccga ccggaccgtt ccaaacgacc gtgcctgcgg
    18601 ctttgagcag ttcggcaagc tcggcggcgg atttcggacc gatgtccaaa atcatatcgt
    18661 cttcggcaac gtcggcaatg tctttcacga ccgcttcggc atcggcggca aaggctttgg
    18721 caacgacgac atcggtcggc agcggcacag aaccgccttt gtccgccatt tttgccataa
    18781 tttttttgga ttcttccact aaatcgtgtt ccgccaaaga tttaccgatg gctttacctt
    18841 cagccaacag gaaggtgttg gcgataccgc cgccgacgat gagttggtca actttatccg
    18901 ccagcgattc gaggatggtc agcttggtgg acactttgct gccggcaacg atggcaacca
    18961 tcgggcgcgc gggctgtttc agggctttgc ccaaagcgtc gagttcgccc gccatcaata
    19021 cgccggcgca ggcaacgggc gcggcttgag cgacggcttc ggtcgaagct tgggcgcggt
    19081 gcgcggtgcc gaacgcgtca ttgacgaaca cgtcgcacaa agaagcgtag gctttaccca
    19141 gttccaaatc gtttttcttc tcgcctttgt tgatacgcac gttttgcagc atcacgacat
    19201 cgcccgcgtt caaagccggt ttgttttcgc gccagtcgtt caatactttc acgtctttgc
    19261 ccaacagacc gcccaaatgc gcggcaacgg gcgcaacgtc gtcttcgggg tggaactcgc
    19321 cttcagtcgg gcgacccaag tggatcatca cgataacgga tgcgccgttg tccatgcagt
    19381 attggatgga cgcgagcgag gcgcggatac gggtgtcgtc gctgattttg ccgtctttga
    19441 acggcacgtt catatcggcg cggatgagga cggttttgcc ctgcacgttt tgttcggtca
    19501 gttttaaaaa tgccataatc agtccttttc aatcagtgtt tgcgatacag aaacaattga
    19561 tgccgtctga aggcttcaga cggcatcgca acccgatcag ccggatacgc gctcgatttt
    19621 cgcgccgacg ctgccgagtt ttttttcaat attttcataa ccgcgatcca agtggtaaat
    19681 ctgttcgacc acggtttcgc cgcgcgccgc caaaccggcg ataacgaggc ttgcggacgc
    19741 acgcaaatcc gtcgccttga cgactgcgcc ggaaagccgt tccacaccct gcacaaatgc
    19801 cgtattgccc tcggttgtaa tgttcgcccc catccggttc aactcgggga cgtgcataaa
    19861 gcggttttca aaaatcgttt ccaccacgcg gcagcttccc tccgccacgg cattcaatgc
    19921 cataaactgc gcctgcatat ccgtggggaa gccggggtgg acgaccgtgc ggatgtccac
    19981 cgccttcgga cgctgccgca tatcgatggc gatccaatcg tcgcccgcct caatcaccgc
    20041 acctgcctca accagtttgt ccaacaccac ttccatcgtt ttcggcgcag cattccgcaa
    20101 aaccaccctg ccgccggtta tcgccaccgc acacaggaac gtccccgctt caatccggtc
    20161 ggggacaacg ctgtgttcgc agccgtgcag ctcgtccaca ccttccacaa tcattgtgga
    20221 cgtaccgatg ccgctgattt tcgcgcccat tttgaccagg cattccgcca aatcgaccac
    20281 ttcaggctcg atggcgcagt tttccaaaac cgtcgtacct tccgccagcg tcgccgccat
    20341 cagcaggttt tccgtgccgc cgacggtaac gacatccatt gccacacgcg tgcctttaag
    20401 cttacccttg gctttgacgt aaccgtgttc gataacaatc tcagcaccca tcgcttctaa
    20461 gcctttcaaa tgctggtcga cagggcgcga accgatggcg cagccgcccg gcaggctgac
    20521 ttgcgcctcg ccgaaacgcg ccagcgtcgg gccaagcacc aaaatcgagg cgcgcatcgt
    20581 ccggaccaat tcgtaagggg cgcaggtgtt gtttaccgta ccaccgttga tttcaaattc
    20641 actgatattg tcggtcagga cgcgcgcgcc catcccctga agcagctttt gtgtggtttt
    20701 cacatctgcc agcataggga cgtttttcag gcgcaacgta cccgatgtca gcaaacccgc
    20761 gcacatcagc ggcaatgccg cgtttttcgc gcccgagacc gttatttccc cgttgagcgg
    20821 gccgtttgcg gagattttca gtttgtccac gtttgttctt tcctagtggg tacttgtaat
    20881 atttcaatac tcgggacaac gcataaagca tcacccgatg aaggttacag aggcggaatt
    20941 ataagggatt ttcgggaaaa atacggaagc cgcaccaaag aatttgacga aatgccgcgc
    21001 tttccgaaca aggattgtcg gaagacaaaa aaagccgagt tttgaaaact cagctttttt
    21061 tgttttatct ggtgggtcgt gagcgattcg aacgctcgac caacggatta aaagtccgct
    21121 gctctaccga ctgagctaac gacccgataa gctcgaaatt ctatacatca agcctgatgc
    21181 tgtcaaccat tttgtcggcg ctcatacggc attttattta tcggactgtt ttttcgtata
    21241 aattaacgag gtcagtatcg atgcacccaa cgcgccgaac acgaccgaca gcgaaacgga
    21301 aatcgggata tgcacccaat gcattaccag cattttcaca ccgataaaac ccaacacgaa
    21361 tgccaagccg tatttcagga agataaagcg ttccgccaca tccgccagca ggaaatacat
    21421 cgcccgcaag cccaaaatag cgaaaatatt ggaagtcagc acgataaacg gatcggtggt
    21481 aacggcaaag acggcgggga tgctgtccac ggcaaacacg acatcgctca attcaatcat
    21541 gaccagcacc aaaaacagcg gcgtggcgat ttttttaccg ttttcgacag taaaaaattt
    21601 ctcgccgtga aattccgtgc cgaccggaac gactttcttg acggcattca gcagcctgct
    21661 gtttgccaaa tccccttcct catcgccttc gggcttcatc atgtgtatac cagtatagag
    21721 caggaacgcg ccgaacagat acagaatcca ctcgaactgc tgaaccagtg ccgcgccgac
    21781 gaaaatcatg acagtgcgca ataccaatgc gcccaatacg ccgtacagca gcacgcggtg
    21841 ctgaaactgc ggtgcgactt tgaagtagcc gaatatcatc aggaacacga aaatattgtc
    21901 gactgccaac gatttttcca aaatgtagcc ggtaaagaat tccaatactt tttcttttgc
    21961 gactgccgcg ccgtagccgg gattgccggc gagtttgaaa tacagccagc ctgcgaacag
    22021 gcaggatacg gcaacccaca agccgctcca tgccaaggct tctttgacgc cgactttatg
    22081 gctgctgttt ttcttcagcg aaaacatatc caaggcaatc atgaccagca ctgccgcaaa
    22141 aaaaacgccg taaaacaacg gcgacccgat gccgggatat tctgtcatgg ttcaatctcc
    22201 tgatttgaaa tgtagttgtg ttaccagctg atataaaaca tcgcttttgc caaaaagaca
    22261 atcagcagca tatgggtaaa gacgacggtg tgtatgtatt tcgaccagcc gacggtcagt
    22321 gtggaacgcg ccattttgac gacggcgatg gcgaagtgcg ccaacacgct gaacgccaac
    22381 aggattttca gcgtcagcat cgtaccgaag gaagtggcaa acggttcgcc caatatagaa
    22441 agatagcggt ttgccgccat cacgatgccg ctggcgaaca gcagtccgac cacaaacggc
    22501 atcaccctga cggcgcggta agacattgcc ttttccactt cgcgccgcgc ctcgcacgac
    22561 acccgtcccg tatgcaggac ggacaaaacc agcacttcaa aaaacacgcc gccgacaaag
    22621 gcgatggcgc aatacaggtg gacgatgtgc gcgacggcat aaatactcat acgatgctcc
    22681 aaccggaaaa ctcggatacg gattgtatca ctatcgtccc cgatgtccgc ataccgcttc
    22741 ccgtaccgcc tcggcgattc tcgcgcccgc tccgcgatgt tgtgcgataa agccgtccac
    22801 gtgcgcctgc atctgcattc cgcccccctc ggacgataag gttttttcaa cggcttcccg
    22861 ccacgcgtcc gccgactcaa cccgaaccgc cgcccccgat gccaaggcgt gccggcaggc
    22921 ttcggaaaaa ttgtaggttg aaaagccgaa tatcgtcgga acgccgcagg aaagcggttc
    22981 gataatgttc tgacagcccg aatcgaccag actgccgccg acaaaagcga catcggcgca
    23041 caggtaatac gcatacagct cgcccatact gtcgcctatc cacacctgcg tatcaggttc
    23101 gaccggcaaa ccgtcgctgc gccgctgaac cttaaacccg aagcgttttg ccgtttcaaa
    23161 taccgtctga aaatgctcgg gatggcgcgg cacgacgacc agcagcgcat cgccgcgata
    23221 ttgttgccac gccgccagca gtttttccgc ctcgtcttca ccccgataaa cgcgcgtgct
    23281 gccgcacacg gcaaccggcc tgcctccgat gcgtttttca aactgccccg ccagcgtttt
    23341 catctgttcc gacggcatca ggtcgtattt ggtattgccg cacacctgca cggatgccgc
    23401 gcccaatttc gccaaccgcg ccgcatccgc ctccgtctgc gccagacaac ccgtcagcga
    23461 agcggcggca ggacggatca ggcggcggac tttcagataa ccgctcaacg atttttccga
    23521 cagccgcgca ttcgccaaaa acagcggcac acccgcgcgc cggcattcct tcatcagatt
    23581 gggccagatt tcggtttcca tcaaaatgcc gaacatcggg cagtgttcgc gcaaaaactg
    23641 ccgtacccac gtttttttgt catacggaag atagcggcat tgcgcatcgg gaaacagaac
    23701 ttgcgcggtt tcccgtcccg tcggggtcat ctgcgtcatc agcagcggtg catcgggaaa
    23761 acgctgccgc aactcgcgta tcaagggctg ggcggcacgc gtttctccga ccgaaacggc
    23821 gtgtatccaa accgcgccgg taacgggatt cggatacggc ttgccgaaac gctcgtcccg
    23881 atgcgcccga tatgcagggg cacttccgga gcgtttgtcc aaataacgcc gtatccatat
    23941 cggcgcaagc agccacaata catcataaag ccattggaac atctttctat ttcctgcaaa
    24001 acaaatgccg tccgaacggt tcggacggca tttcggcaac ggaatcaaat atcgtaggtt
    24061 gtcgaagcgg tatctccgcc cttgcccgtc cagttggtat ggaaaaactc gccgcgcggt
    24121 ttgtcggtgc gttcgtaagt gtgcgcgccg aagtagtcgc gctgtgcctg caagaggttg
    24181 gcaggcagac gttcggtcgt gtagccgtcc aagaacgtaa tcgccgaagc catgcagggc
    24241 atagggatgc cgcattcgac tgccttggca accactttgc gccacgccgg caggcagttt
    24301 tccagaatat ttttgaaata cggatccgta cccaagaaca ccaaatcggg attgttttca
    24361 tacgcgtcgc ggatattgct taagaatgcg ctgcgaatga tgcacccctc gcgccacagc
    24421 agcgcagtgt tgccgtagtc caaatcccag ccgtagcttt cgcccgcttc gcggatcagc
    24481 ataaagcctt gtgcgtagga aatgattttg gatgcaagca gggcctgtct caacgcctca
    24541 acccattctt gtttgccgcc ttcgacgggc gtaacggttc gggcgaacag cttgccggtc
    24601 tgcacgcgct gttctttgaa cgacgaaacg cagcgggcga ataccgcttc ggaaatcagc
    24661 gtcaacggaa tacccaaatc caaagcattg atacccgtcc atttgcccgt gcctttttgc
    24721 cctgccgtat cgaggatttt ctcgaccagc ggttcgccgc cttcgtcttt atagcccaaa
    24781 attgccgctg tgatttcaat cagataagaa tccagctcgg ttttgttcca cgcggtaaac
    24841 acgcggtgca tttcgtcgta ggacagtccc aaaccgtctt tcataaactg gtacgcttcg
    24901 caaatcaact gcatatcgcc gtattcgata ccgttatgca ccattttgac aaaatgtccc
    24961 gcgccgtccc tgccgaccca gtcacaacac ggttcgccct gcggcgtttt ggcggcaatc
    25021 gcctgaaaaa tcggtttgac ggcttcccaa gcgcgcttat ccccgcccgg cataatggac
    25081 gggccgtgcc gcgcgccttc ttcgccgccg gatacgcccg ccccgacaaa caaaatccct
    25141 ttttcggcaa ggtaatgtgt ccgccgtgtc gtgtcggggt aattggcatt gccgccgtcg
    25201 ataatgatgt cttcctcgtc caacaggggc agcagttgtt cgataaagtc gtcgacgacc
    25261 gaacctgcgc gaaccatcat catgattttt ctcggttttg ccagcttatc gaccaagtct
    25321 tgcagggaat acgcgccgac aatatccgtt cccttagccg cgccgtttaa aaattcgtcc
    25381 accttaccga ttgttcggtt gtaggcgaca accttaaatc cgcaatcgtt catattcaaa
    25441 atcaggtttt gccccataac cgccaaaccg attacgccaa tatcgccttt cattgcagga
    25501 agctccgtta tagatttaat ttatcgaccg caactctacc tgatttacac ttgtttaaca
    25561 atccttaact ctttaatttt ttgaaaagat gcctttacgc ttttccgtgc gtttccctga
    25621 aggcttataa ataaaatata aaatttaaat aataaaacga tgattatatt gataggagga
    25681 attttctgtg ggtaactttt ttttatttta aaaatcattg ggatttccgt tttaaaaggg
    25741 tgtcggtaag gcggattccc ttttgtgcat acctgtggat tgtttttcat gaagaatagt
    25801 ttttgtggac agtttgcttg ttgtgcagat ggcatcctac ttttctttac cgaatggctg
    25861 ccggtgcctt taagaacggg aatactgtgg agatttaagc cggaagtgtg tttggaacgt
    25921 gtggaaatgg tcaggtgtcg gcacgaatgt cttatttctg catatcggca gagtgcgcat
    25981 ccgaatttgt gtataagtgg tggaaaaaat gagatttgcg ggtaaatctc acaatatttc
    26041 agtctgataa ctttggattg cttgtgtata aataaacttt cggatgggga tacgtaacgg
    26101 aaacctgtac cgcgtcattc ccacgaacct acatcccgtc attcccatga aagtgggaat
    26161 gatgaaattt tgagttttag gaatttatcg gaaaaaacag aaaccgctcc gccgtcattc
    26221 ccgcgcaggc gggaatccag cctcgtcggt acggaaactt atcgggaaag cggtttcttt
    26281 agattttgcg ttctggattc ccacttttgt gggaatgacg ggattaaagt ttcaaaattt
    26341 attctaaata actgaaactc aacgaactag attcccgctt ttgcgggaat gacgaatttt
    26401 aggtttctgt ttttggtttt ctgtttttga gggaatgatg aaattttaag tttgaggaat
    26461 tta
//
LOCUS       DAWFKN010000020        26096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFKN010000020 DAWFKN010000000
VERSION     DAWFKN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.020"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(207..494)
                     /locus_tag="WGR46_000857"
     CDS             complement(207..494)
                     /locus_tag="WGR46_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855833.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(801..1172)
                     /locus_tag="WGR46_000858"
     CDS             complement(801..1172)
                     /locus_tag="WGR46_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855834.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(1306..1749)
                     /locus_tag="WGR46_000859"
     CDS             complement(1306..1749)
                     /locus_tag="WGR46_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855835.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1792..2661)
                     /locus_tag="WGR46_000860"
     CDS             complement(1792..2661)
                     /locus_tag="WGR46_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0855836.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2825..3748
                     /locus_tag="WGR46_000861"
     CDS             2825..3748
                     /locus_tag="WGR46_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0855837.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3826..4467)
                     /locus_tag="WGR46_000862"
     CDS             complement(3826..4467)
                     /locus_tag="WGR46_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0855838.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4522..5313)
                     /locus_tag="WGR46_000863"
     CDS             complement(4522..5313)
                     /locus_tag="WGR46_000863"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0855839.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5479..5554
                     /locus_tag="WGR46_000864"
     tRNA            5479..5554
                     /locus_tag="WGR46_000864"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5512..5514,aa:Thr,seq:tgt)
     gene            5589..5664
                     /locus_tag="WGR46_000865"
     tRNA            5589..5664
                     /locus_tag="WGR46_000865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5622..5624,aa:Thr,seq:tgt)
     gene            5806..6597
                     /gene="panB"
                     /locus_tag="WGR46_000866"
     CDS             5806..6597
                     /gene="panB"
                     /locus_tag="WGR46_000866"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0855840.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6587..6763
                     /locus_tag="WGR46_000867"
     CDS             6587..6763
                     /locus_tag="WGR46_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855841.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6720..7556
                     /locus_tag="WGR46_000868"
     CDS             6720..7556
                     /locus_tag="WGR46_000868"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0855842.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7721..9559
                     /locus_tag="WGR46_000869"
     CDS             7721..9559
                     /locus_tag="WGR46_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855843.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9699..10280
                     /locus_tag="WGR46_000870"
     CDS             9699..10280
                     /locus_tag="WGR46_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0855844.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            10290..11135
                     /gene="ispE"
                     /locus_tag="WGR46_000871"
     CDS             10290..11135
                     /gene="ispE"
                     /locus_tag="WGR46_000871"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0855845.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            11143..11218
                     /locus_tag="WGR46_000872"
     tRNA            11143..11218
                     /locus_tag="WGR46_000872"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11176..11178,aa:Gln,seq:ttg)
     gene            11231..11306
                     /locus_tag="WGR46_000873"
     tRNA            11231..11306
                     /locus_tag="WGR46_000873"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11264..11266,aa:Gln,seq:ttg)
     gene            11315..11390
                     /locus_tag="WGR46_000874"
     tRNA            11315..11390
                     /locus_tag="WGR46_000874"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11348..11350,aa:Gln,seq:ttg)
     gene            11557..12540
                     /locus_tag="WGR46_000875"
     CDS             11557..12540
                     /locus_tag="WGR46_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0855846.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12607..13179
                     /locus_tag="WGR46_000876"
     CDS             12607..13179
                     /locus_tag="WGR46_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0855847.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            13513..15504
                     /locus_tag="WGR46_000877"
     CDS             13513..15504
                     /locus_tag="WGR46_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0855848.1"
                     /translation="MDGRRNYGNRTEPNRTEPNRTEPNRTEIIHDLENNFKQNNILKL
                     KQLFPEVFCEDQIDFEKLKLVLGAENLAGAGERYQLDWAGKTAAYLNLQSPTSRTLVP
                     CKEESANFDGTQNVFIEAENLEALKILQKAYAGSVKMIYIDPPYNTGSDSFIYPDKFS
                     ESRDEYARRVGDTDDAGYLKRDGVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTLL
                     REDGVIFISIDDNEQAQLKLLCDEVFGAENFVNQIAVKMSELSGVKMKHLNQYAKLKE
                     FLLIYAKNIHFANFNIEKKRKSPETLSKYLKYYSSIIENIESECEQWKIISLDEYFKD
                     KNIILDREKLNDWKLSNAQRLVYRTNSKTVDKFLLKNPNAPDICKLINDDGKEIIKWG
                     NKEMLFLEKYIDEYLGDIWLDISTINLNKETHTLVFENGQKPLTLIKRLLKSLETENN
                     IVMDFFSGSGTTAHAVMQLNAEDGGNRRFICVQLPEETDEKSEARKAGFDTIAEIAKE
                     RIRRAGRQISDGLQDGSETDTGFKVFKLAESGFKQWRQPEQSDTEALQRELSLNIDSV
                     LSETSSENLLYELMLRMGLKLTCKVSFLDDVYFVEDEDTGGLYAFLLKRVDQGLIDAV
                     LAKHPAKVAALDRLFEGDDALKSNTVLQMKDAGVMFECV"
     gene            15739..18390
                     /locus_tag="WGR46_000878"
     CDS             15739..18390
                     /locus_tag="WGR46_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0855849.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGLNLNTVQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNR
                     SYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRTFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGETPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKQPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFVEL
                     LEVKQAAKGRPKADLNIHYQDKKETKKKKVSVKWEDDLFEKSGGNEAYRHGFIIDGMD
                     FDKQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELAGNTDTAGVHDGYFSQDRKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWDKLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIAGRSREMDVQWEIPDILGEIQRKTGLTRRTVYEII
                     IQSGRLKDVAGNPQRFIDLTAEAVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENGFTFTVSNPQKTICENYIPLDSGTEKAFAQNCEDYDAKNEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVRDKVYFVAETKNTGKGIQTGVDMDKLRQNERLKIKCGKR
                     HFEALGGLAYRVVEKVGDLKSEKEEQA"
     gene            complement(18552..19721)
                     /locus_tag="WGR46_000879"
     CDS             complement(18552..19721)
                     /locus_tag="WGR46_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0855850.1"
                     /translation="MTAHKILPVLLSIILGVSHTTAASPAPNKLTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWVRLTGQ"
     gene            19869..21395
                     /gene="ilvA"
                     /locus_tag="WGR46_000880"
     CDS             19869..21395
                     /gene="ilvA"
                     /locus_tag="WGR46_000880"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0855851.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(21452..22525)
                     /locus_tag="WGR46_000881"
     CDS             complement(21452..22525)
                     /locus_tag="WGR46_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0855852.1"
                     /translation="MSITIQNLNKHFGAFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETHAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQLAAENETPMICAEIEKIHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(22522..23382)
                     /gene="cysW"
                     /locus_tag="WGR46_000882"
     CDS             complement(22522..23382)
                     /gene="cysW"
                     /locus_tag="WGR46_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0855853.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAA"
     gene            complement(23637..24473)
                     /gene="cysT"
                     /locus_tag="WGR46_000883"
     CDS             complement(23637..24473)
                     /gene="cysT"
                     /locus_tag="WGR46_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0855854.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            24612..24719
                     /locus_tag="WGR46_000884"
                     /pseudo
     CDS             24612..24719
                     /locus_tag="WGR46_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            24764..25096
                     /locus_tag="WGR46_000885"
     CDS             24764..25096
                     /locus_tag="WGR46_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0855855.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            25428..25937
                     /locus_tag="WGR46_000886"
     CDS             25428..25937
                     /locus_tag="WGR46_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0855856.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         6679 a         5905 c         7006 g         6506 t
ORIGIN      
        1 aatttactcc actatacaaa acaatcaaga aactattggc ggtatgtata atcttgaatt
       61 tctttttttg atgaaattaa aataatgaaa tatatggtaa acgttacaac ggaagcctta
      121 gcctcccgat tgcagtattt caattccaag ataaatgccg catagaagat aaaatactca
      181 aatggttttt tagcggctta aatatcttat ttaatcttat tgattgtttt cccaatggtt
      241 tccgcaataa ataataagaa gtagaataat aaacaaacag aacatgaaaa taaaaataaa
      301 tctttcatta tcccagtaat aaaactccaa agattttcct tatttccatc aacaataaaa
      361 attatcagtt catgaataaa gaatactgca aagaaaatgc ttcctgccgt tagagatatt
      421 tctaatctgc ttattttccc ttcgctatat ttacctgata aacagcctaa aaaagctgca
      481 atcaaatcat ccattttaaa aatttctgta aaaacaggta atgtctttaa ttcattacct
      541 gttttgccat tatttatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga
      601 cagtacaaat agtacggcaa ggcgaggcaa cgccgtacta atccactata tatatccatt
      661 catatagaga aaaattctta tacccaatat aaaacaaggt ctttcattat attttgaaaa
      721 acctagaagc tttgtatcta ttggtttatc aaaattaata attattgcag aagacatgta
      781 ggctaataat cttgatttat ctatatttta ttaattatat atttaacccc gaagtcaata
      841 tagaaattta gtgattttaa agttctctta tcaatttcaa tctcagggag tacataaaaa
      901 tcctcagaat aggcaaactc tatatatgca tcatcattcg ataataattc aataatattt
      961 ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac atttattttt agataccatt
     1021 tttgaatatt gattaaataa ttttggatta ctatttttag taattaatcc ttccggagta
     1081 ataaaaccac tttgtataaa gtcatcactt atattaacag ccttccgaaa aaaaatataa
     1141 tcacactctt tgttatcaat ataaaatctc atactcttta ataatgaaat ataagaattt
     1201 ccagaattac aagcaatttg aaaaaacatc attactccta aaactcataa atagtaagga
     1261 atatatatat atgcatattc cttactattt tttcacaaat tatgcttatc tatgcttatt
     1321 tccataaaaa gcatgccctc gaattttttg acttgagttc tggaaatgag aataatttga
     1381 atggatttcc ttctgtccat gtctaataac attttgtctt ccccaagctt ggtttgctac
     1441 cgctcttgct tgttgagatg ttcgtgcata gacattagct cctcttgata aagcttgtgc
     1501 tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg cccgcatgca aaattcctat
     1561 tgccaccaac ggtgccgcag ccccttcagt ttctttcatc tccttttgag aaagctccgc
     1621 caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg ttttcaaatg tctgattgtc
     1681 caagccgttt gccattgcgg gggcaatcat agacagcatc attacggctg cggtgatttg
     1741 ttttttcata ataactcctt tggattacaa ggttggaaaa tcaaaacctg cttaaaacgt
     1801 atgctgtacg ccaaatttca gttcggaact gctttgccct gaaacgttga aacgtgcgga
     1861 tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg ccgaaatgag cgtaggtgga
     1921 tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg ggctgtgtgc ccagccattg
     1981 gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat gagatgttgg ggttgagcag
     2041 caggtagttg cccgatttgt agcggatgcc gtctgaaagg gttttgctgc cgttgatgcg
     2101 gtaggcggcg gtgagggaaa ggacaatcgg atctatggct ttgtaggtgg tggcgccgat
     2161 gagccaggat tttcccgacg aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag
     2221 aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa gtgtggctga tgccgaggga
     2281 tacgtcggac atccgtttgt tgcgggtttt gccgttgccg tcgagtttgc gttcttcgtg
     2341 ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg gtcagtccgt agcgcaaacc
     2401 gagcgtgccg acgagcatat cggtattgct gccgttttct tggatttcgg tcggaatggg
     2461 gataaacgag gttgcgccgg tttgaatgta aaccggtgcg gcaagttcgg cgcggttgtt
     2521 ttcgctgttc aggtaggtaa gggaagtttc cagtttccat tttcccttgt cggtcattat
     2581 gtcttcaatc gtcaagggca ggtcggcata agtggataaa ggcaggatgg cgggcaaggc
     2641 gggcaaaaag atgcgcttca tatttctcct attgatatta attcttattt actgataata
     2701 atgaatatcc gataatcttg tctaaataaa tttttaaatc ataaaaaaca atatgtttgt
     2761 ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg tgaaaaacgg ttacaatccg
     2821 ccgcatgaaa aaacgcaata atcctcttcc tctgttgaac ggtgtcaaac ccagttattt
     2881 ggtgctgccg catgaaaagc agttttacgg gctgccgctg ctgcattttc tgtgcatccg
     2941 ctttcctttt gtgggcgcgg acgattggcg caggcggttg aacagcggtt ttgtggtcgg
     3001 ttcggatggt gcgacgttgg acgaacatac tttgttcgag ccgggtaagg tgatgtttta
     3061 ttatcgggaa accagcagtg aggatgagcc gcgtattccg tttgaagaaa agattttgca
     3121 tattgatgag catttgattg tggtggacaa gccgcatttt ctgcccgtca tccccagcgg
     3181 caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg cggcctgaat tgcagcattt
     3241 gaatgttgag gacattacgc cgctgcaccg cttggacaag gatacggcag gcgtgatgct
     3301 gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg atgtttcaaa acaaaacggt
     3361 atggaaaacg tatgaggcgc ttgcgccgac aaggacggat ttgccgtatc cgctcgatgt
     3421 ggtttcgcgt ttggtgaggg gtgagaaatt ttttacgacg caagaggcgg aaggcgaacc
     3481 aaacgcacat acgacggtcg aactgattga aaacaggggg gaattcagcc tttaccgcct
     3541 tacgccgcat acgggcaaga aacaccagtt gcgcgtgcat atgatgggtt tgggtatgcc
     3601 gctgttgaat gacgcgctct atcccgtgcc gtctgaagcg ggcagcgagg attatcggaa
     3661 acctttgaag ctattggcaa aaaagattgc gtttgcagat cctttgtcgg gtcgggaaag
     3721 ggtgttttgc agcggatttt gcctataatg agagggaacg caaacccgta tcggcggatg
     3781 ccgccgatac gggttttaca ttatcgatgc cgtctgaaag gggttttatc cgttcaggca
     3841 tacgggcgcg aagcggagga tggtgtcgcg caggacgatg agtttgccgt gaaagaaatg
     3901 cgtggaaccg gcgatggtaa tgacgggcaa atcttgcggt tccgcccatt tcagtgcttt
     3961 cccgatttcg acgacttcgt cttccgcgcc gtgtatcatc agcgttttgg caacattggg
     4021 aacggcggac ggctcggggc ggccggtata gtggcagact gccgcgccga tgagcagcaa
     4081 taaatcgggt atgcgcgctt gtgcggcaaa tgtggcgaca taaccgccga aggagaagcc
     4141 ggataaggca aattcggggg cttcgggatg ttgggcacgg gcatagtcga tgacggcgag
     4201 gcagtcttgc gtttcgccgc gtccgtaatc gtgtgtgcct tcgctgccgc ctacgccgcg
     4261 aaggttgggt aggtagcagt ggaagccgag tttgcttaag gctttggcgg cagtttggat
     4321 aactttgttg gtgtttgttc cgccctggag ggggttggga tgattgatga cggcaacacc
     4381 gcgtgccggt acttgttcgg acgggatatg gatggtttct aaaatgccgg caggaccgga
     4441 tatgtgtatg gtttcgggtt tcagcataat gatttcctct tgattttccg catgttccaa
     4501 tgcagccgtg aagatagttg cttaaaggtg gaaatgcttg ccgttgttgg ggttggacgc
     4561 tttcaggccg gcaagcatac ggtggaagtc caatccgtat gcgttgctca gtttgtcggc
     4621 acgttttttg agtttgtcta tgttttgctc tttggggctg ctttggaagg ccattaccgc
     4681 gacattgccg tggctttcgg caggaagttc caagacgcgc ccttcaaaaa cgctcaacaa
     4741 ccgttcgatg aagcgttggt agcgtttgtc gccgctccac cagttggtta cgaatatgcc
     4801 gtctgaagag agtgcgtttc ggcagtctcg gaaaaacggt tcttcaacca gcgcatcgat
     4861 gatttgttcg ccgtcgaacc cgtccaccaa aatcacatcg gtgttgtggc ggaagacttt
     4921 gatatattct gcaccgtctg cttcaataat ttcaaatttc tcgccctcga aaggcaactc
     4981 aaacaggttg cgggcaatgg cgatgacctg aggattgatg tccacggcgg tttggcgcgt
     5041 gtcgggcagg taggtgtcta tccagcgtgc aaacgagccg ccgcccaagc cgatttgggt
     5101 gatatgctgc ggaagtgcgt cggtaaacag cagccagccc atcatcgcgc ggctgtaaga
     5161 gagtaccagc tcggacgggt ggtcgaggtt cattgagctt tgaacggtgt cgctgcccaa
     5221 gtgaagcgaa cggatattgc cttcttcgga aatgccgact tcgggaaagc cggattttgc
     5281 aggacgcagg cggcggtagg gatgtcttgc catcggtgtg aacggtcggt ttgaaaggcg
     5341 gatattttat cagaatgccg atgcttgtcc tgcttcaaat taatttcttt tcaaataaat
     5401 tacttattcg gatttgccgg ggctttcgga taaattcctt gccaaagtgt gaagttgcct
     5461 gcataattcg ccttctttgc cggtatagct cagttggtag agcacctgac ttgtaatcag
     5521 ggggtcccga gttcgactct tggtgccggc accatttctg ctgatggtgt tgcaaaacat
     5581 ttcagtaagc cggtatagct cagttggtag agcacctgac ttgtaatcag ggggtcccga
     5641 gttcgactct tggtgccggc accagatttg acagtccgat tgtgtaaaac agtcgggctg
     5701 ttttgcattt gcggcacggt cggtggcagg gctgcgggca tttcactata atagcccgtt
     5761 tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat atctgatgat tactgtgaac
     5821 acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta tgctgaccgc ttacgaatcc
     5881 agttttgccg cgctgatgga cgatgccggc gtggatgtgc tgctggttgg ggattctttg
     5941 ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca gcctgcgcga tatgtgttat
     6001 cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga ttgtcagcga tttgccgttt
     6061 ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg ccgccgaact gatggctgcc
     6121 ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg cggaaacgac cgaatttctg
     6181 caaatgcgcg gtattccggt ctgtgcccac atcggtctga ccccgcaatc cgtgtttgcc
     6241 ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc aggcgttgct taacgatgcc
     6301 aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg agtgtgttcc tgcggaactg
     6361 gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg gcatcggcgc gggtgtcgat
     6421 tgcgacgggc aggttttggt gatgcacgat atgctcggca ttttcccggg taagacggtg
     6481 aaattcgtca aaaactttat gcaggggcaa agcagcattc aggcggcggt tcgggcgtat
     6541 gttgccgaag tcaaagccaa aaccttccct gctgcggaac atatttttgc agactgaaca
     6601 aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg cattttgttt tgccgtgcgc
     6661 ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg aaggataagg attaccgtta
     6721 tgcaaatcat acataccatt caagaactgc gcgcgtggcg taaaaatgcg ggaacggtgg
     6781 catttgtgcc gaccatgggc aatctgcacg aagggcatct tgccctcgtt cgcgaagcca
     6841 aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa ccgcctgcaa ttcggtcagg
     6901 gcgaggattt cgacaaatat ccgcgtactt tgcaacagga tgcggacaag ctggaagccg
     6961 agggtgttgc cgttgttttc gcgcccgatg agaaagaact ctatccgaac gtggaacagc
     7021 gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg cggcaagttt cgtccgggac
     7081 atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa tattgtcgcg ccggatgttg
     7141 cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat taaaggtttg accgaggatt
     7201 tgaattttga tgttgaaata gtgcctgtcg atacggggcg cgcggaagac ggtttggcac
     7261 tctccagccg caaccagtat ttgagtgcgg cggaacgcga cgaagcaccg cgcctgtacc
     7321 gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag tttggattat gcaggtttgg
     7381 aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt ggtcgattat gtcgaaatcc
     7441 gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa gaaactggtg gtcttggccg
     7501 ccgcccgtct ggggacgacg cgcctgattg acaatttgga aataaaactc ccttaaaccg
     7561 caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca gacggcattt atttttttga
     7621 acggggtttc gcaaatctac aaacgattct gcttgtgata aagttacgcc tgattatatg
     7681 ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt atgttacccg cccgtttcac
     7741 cattttatct gtgctcgcgg cagccctgct tgccgggcag gcgtatgccg ccggcgcggc
     7801 ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga aagcagcagc gttacagcga
     7861 ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg ggcgagcggg ttaatcagat
     7921 atttacgttg ctgggagggg aaaccgcctt gcaaaagggg caggcgggaa cggctctggc
     7981 aacctatatg ctgatgttgg aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga
     8041 aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg atttatcaga aatggcggca
     8101 gattgagcct ataccgggtg aggcgcaaaa acgggcgggg tggctgcgga acgtgctgag
     8161 ggaaagagga aatcagcatc tggacggact ggaagaagtg ctggctcagg cggacgaagg
     8221 acagaaccgc agggtgtttt tattgttggc acaagccgcc gtgcaacagg acgggttggc
     8281 gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa tatgaacatc tgcccgaagc
     8341 ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa aaggaaaagg caatcggagc
     8401 tttgcagcgt ttggcgaagc tcgatacgga aatattgccc cccactttaa tgacgttgcg
     8461 tctgactgca cgcaaatatc ccgaaatact cgacggcttt ttcgagcaga cagacaccca
     8521 aaacctttcg gccgtctggc aggaaatgga aattatgaat ctggtttccc tgcacaggtt
     8581 ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc aatccgaatg cagacctgta
     8641 tattcaggca gcgatattgg cggcaaaccg aaaagaaggt gcttccgtta tcgacggcta
     8701 cgccgaaaag gcatacggca gggggacggg ggaacagcgg ggcagggcgg caatgacggc
     8761 ggcgatgata tatgccgaca gaagggatta caccaaagtc aggcagtggc tgaaaaaagt
     8821 gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg gctgcggcgg ctgtcgagtt
     8881 ggacggcggc agggcggctt tgcggcagat cggcagggtg cggaaacttc ccgaacagca
     8941 ggggcggtat tttacggcag acaatttgtc caaaatacag atgttcgccc tgtcgaagct
     9001 gcccgataaa cgggaggctt tgagggggtt ggacaagatt atcgaaaaac cgcctgccgg
     9061 cagtaataca gagttacagg cagaggcatt ggtacagcgg tcagttgttt acgatcggct
     9121 tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg ttcaggcttg cacccgataa
     9181 cgctcagatt atgaataatc tgggctacag cctgctttcc gattccaaac gtttggacga
     9241 aggcttcgcc ctgcttcaga cggcatacca aatcaacccg gacgataccg ctgtcaacga
     9301 cagcataggc tgggcgtatt acctgaaagg cgacgcggaa agcgcgctgc cgtatctgcg
     9361 gtattcgttt gaaaacgacc ccgagcccga agttgccgcc catttgggcg aagtgttgtg
     9421 ggcattgggc gaacgcgatc aggcggttga cgtatggacg caggcggcac accttacggg
     9481 agacaagaaa atatggcggg aaacgctcaa acgtcacggc atcgcattgc cccaaccttc
     9541 ccgcaaaccc cggaaataat gcaggtccat cctttcagac ggcataaggt ttgccgggaa
     9601 gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt tttgcacggc acgccgaacc
     9661 cgccggccgg caggatggca cccgttaagg aaattctgat gaaacacacc gtatccgcat
     9721 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
     9781 agccgtccga acacatcagc agttttgcag cagaagggcg gttggcagtg aaagcggaag
     9841 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
     9901 tcaatacccc tttgggcagt acgctcgggc agttgtgtca agacagggac ggcgcattgg
     9961 cagtggacgg caaaggaaat gtctatcagg cggaaagtgc ggaagaattg agcaggcagc
    10021 tggtcggttt caaactgcca atccaatatc tgcatatctg ggcagatggc aggcgtgtgg
    10081 cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga acaatacggt tggactgtcg
    10141 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
    10201 acatcaggct ggttttcacc gaaatcggta tgccgtctga aactgaaacc ccggaacgct
    10261 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
    10321 ccgcacctgc aaaactgaat ctcgatttga ggattaccgg caggcgggaa gacggttatc
    10381 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaatcga
    10441 gggatgacgg caaaatcatc ctgcacaatc cggttgatgg aatgccgcag gaagcggatt
    10501 taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa
    10561 tatggctgga caaaaaaatc ccgacagggg cgggtttggg cggcggaagc tcggatgcgg
    10621 caaccgtttt gctggtgttg aaccgttggt ggcagtgcgg tctgacgcag cggcagctca
    10681 ttgattcggg cgcggctcta ggggcggacg taccattttt tattttcgga aaaaatgctt
    10741 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtca
    10801 tcgtcaaacc gcccgtccac gtttccactg caaaaatttt cacacacgaa ggcttgacac
    10861 gaaattccgc ctcaagcata atgccgactt tccaaaacct gcaaccgttt agaaatgaca
    10921 tgcaggcagt tgtatttaaa gaataccctg aagtttggaa agcctattcc gaattgtccc
    10981 gatatggatt tgccttaatg acaggttctg gtgcgtgtgt attcacggcg tgtcaagata
    11041 ggaatagcgc atacaatata taccgacaag tttcagattt gtacgaggca tatttggcag
    11101 agggtctttc aaaacatcct ttgttgtccg tataaacatt gttggggagt cgtcaagcgg
    11161 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccaag
    11221 tcaaacaagt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
    11281 aggttcgaat ccttcctccc cagccatatt tttttgggga gtcgtcaagc ggttaagaca
    11341 ctggattttg attccagcat gcgaaggttc gaatccttcc tccccagcca gataaaagcg
    11401 tgtaagcctg cttacacgct tttatttcat agaaataaaa atattgaaat gcctttgttt
    11461 gtgtcggatg ttgcaggtat aatgtcgggc ttggtacaag cagagggaag cattgtgttt
    11521 tctgagcgga agttaaacat aaaatcaggt gagaatatgg ctgcgtacga cagtttgatg
    11581 gtatttacag gcaatgccaa tcccgaattg gcacaacgtg ttgtcaggca tttggacatt
    11641 tctttgggca atgcttccgt atccaagttt tcagacggcg aagttgccgt cgaactgttg
    11701 gaaaacgtac gcgggcgcga tgttttcatc cttcagccga cctgtgcgcc gaccaatgac
    11761 aacctgatgg aaatcctgac gatggcggat gcactgaagc gtgcttcggc aggtcgtatt
    11821 accacagcca ttccgtattt cggctatgcg cgccaagacc gccgtccgcg ttccgtccgc
    11881 gttccgattt ctgccaaact ggtggcaaat atgctgtatt cggcagggat cgaccgtgtt
    11941 ttgactgtcg atttgcatgc cgaccagatt caaggtttct tcgatattcc ggtggacaat
    12001 atttatgcca ccccgattct gttgaacgac atcaaacaac agcggattga aaatctgacc
    12061 gtcgtcagcc cggacatcgg cggtgtcgtc cgcgcccgcg ccgtggcaaa atccttgaat
    12121 gccgacttgg caatcatcga caaacgccgc ccgaaagcca atgtggcaga agtcatgaac
    12181 atcatcggcg atattcaagg cagaacctgt ctgattgtgg acgatatgat tgacactgca
    12241 aatacgctgt gcaaagccgc cgtcgctctg aaagagcggg gggcggaacg tgtcttggca
    12301 tatgccagcc acgccgtatt ctctggagag gcggtcagcc gtatcgcctc atccgaaatc
    12361 gaccaggtgg tcgtaaccga taccattcct ttgtctgaag cggctaaaaa ctgcgaccgt
    12421 atccgtcagg taacgattgc cggtctgttg gccgaaaccg tccgccgcat tagcaatgaa
    12481 gaatccgtct catatctttt caatgaagaa gtgatgacag gcagcatgtt gctgccataa
    12541 gcccgaagcc gtcttaagct ggtcgcggcc gatgacggcg attttaccta acttggagta
    12601 tttaacatga cttatgaaat tcaagcttct gttcgtgaag cacaaggcac tggtgcgagc
    12661 cgccgcctgc gtcgcgaagg ccaaatcccc ggcattctgt acggtgaagg tcaagagccc
    12721 gttgccattg ccgtggatca caaaaccgta ttctacgcat tggaaaaaga atctttccat
    12781 accgcgttga ttaaattgtc tctgaacggc gaaaccaaag acgttatcgt ccgtgatttc
    12841 caaatgcacc cgttccgccg cgaagtccaa cacatcgact tccaagccgt taaagccgat
    12901 cagcttgtac gcatccgtgt tcccctgcac atcgttaacg ctgaaaattc ccaagcggtc
    12961 aaactgcaag gcggtcgtgt atctctgttg aacacgacgg ttgaagtgct tgcgttgcct
    13021 gccaatatcc ctgctttctt ggatttggat tgtgccgaag tggttgccgg cgacattctg
    13081 cacttgtcag acatcaaact gcctgaaggt gtagaaagcg tttccctgaa acgtaacgaa
    13141 aatctggctg ttgctactgt taccggtaaa aaacgctaat tgattgaaaa gcaggcggta
    13201 cgtatatgat gcgtaccgcc ttattgtttt gggctgtgtt tcaatatttg gttgatttca
    13261 tccgtttata agggaaaacc gcttattttc gtttgggtag aaatctatct aggcgtattg
    13321 cgcgacaacg gatggttagc ttaaaaacgg catttcgtac cggatgaaaa tgaagaagat
    13381 atgtcgttca cattccggta gctgcccggc tggaaccggc tatcagagat agttggtcaa
    13441 ctttcaccct gaaaatcact tttcttttaa cttgcaacat attccgatag aatggacact
    13501 ttgtaagtaa tcattgatgg aagacgcaat tatggcaacc gaaccgaacc gaaccgaacc
    13561 gaaccgaacc gaaccgaacc gaaccgaacc gaaattatac acgatctaga aaataatttc
    13621 aaacaaaaca acatattaaa actcaaacaa ctctttcctg aagtcttttg cgaagaccaa
    13681 atcgactttg agaagctcaa actcgttctc ggcgcagaaa accttgccgg tgcgggcgaa
    13741 cgttatcaac tcgattgggc gggtaaaacc gccgcctatc tcaacctgca aagtcccact
    13801 tcccgaaccc tcgttccctg caaagaagaa tctgcaaatt tcgatggcac ccaaaacgtg
    13861 tttatcgaag ccgaaaacct cgaagcactg aaaatcctgc aaaaagccta cgccggcagc
    13921 gtgaagatga tttacatcga cccgccctac aataccggca gcgattcttt tatttatccc
    13981 gacaaattct ccgaaagccg cgatgaatac gcccgccgcg tgggcgatac cgacgacgcg
    14041 ggctacctga agcgcgacgg cgtattccaa ggcgcgtggc gcaaaaacgg caaagacagc
    14101 ggccattacc acagcaactg gctctccatg atgctgccgc gcctacatct ggcgaaaacc
    14161 ctgctgcgcg aagacggcgt gatttttatc agcattgacg acaacgaaca ggcgcagttg
    14221 aaattgttat gtgatgaagt gtttggggcg gagaattttg tcaatcaaat tgctgtcaaa
    14281 atgagtgaac tttctggggt taaaatgaag catttaaatc agtatgctaa attaaaagaa
    14341 tttttactga tttacgctaa aaatattcat ttcgctaatt tcaatattga aaagaaaaga
    14401 aaatctcccg aaactctatc taaatattta aaatattatt catcaataat agaaaatatt
    14461 gagagtgaat gtgagcaatg gaagataata tcactagatg aatattttaa agataaaaat
    14521 atcattttag atagagagaa attgaatgac tggaagctat caaatgcaca acgcctagtg
    14581 tatcgtacaa acagtaaaac cgttgataaa tttctactta aaaatccaaa tgctcctgat
    14641 atttgtaaac tcatcaatga tgatggtaag gaaattatca aatggggtaa caaagaaatg
    14701 ttattcttgg aaaaatatat tgatgaatat ttaggcgata tttggttgga tatatctact
    14761 attaatttaa ataaagaaac tcatacctta gtatttgaaa atggacaaaa acctttaaca
    14821 ttgattaaaa gattactaaa aagtttagaa actgaaaaca atattgttat ggacttcttc
    14881 tccggcagcg gcaccaccgc ccatgccgtg atgcaactga atgccgaaga cggcggcaac
    14941 cgccgtttca tctgtgtcca gctccccgaa gaaaccgacg aaaaatctga agcgcgcaag
    15001 gcgggtttcg acacgattgc cgaaattgcc aaagaacgca tccgccgcgc aggcaggcag
    15061 atttcagacg gccttcaaga cggatcggaa acagatacgg gctttaaagt tttcaaactt
    15121 gccgaaagcg gtttcaaaca atggcggcag cccgagcagt ccgatacgga agccctgcaa
    15181 cgcgaattgt cgttgaatat cgattctgtg ctgtccgaaa cgtcgtctga aaatctgctc
    15241 tacgaattga tgctgcgtat gggcttgaag ctgacttgca aggtttcgtt tttagacgac
    15301 gtatatttcg tggaagacga ggatacgggc gggctgtacg ctttcctgct gaaacgcgtg
    15361 gatcaaggtt tgattgatgc cgtgctggcg aagcatcccg ctaaggtggc ggcattggac
    15421 aggctgtttg agggcgacga tgcgcttaaa agcaataccg tattgcagat gaaggatgcg
    15481 ggcgtgatgt tcgagtgtgt gtagtcagcc gaaagcggga agtgccgtct gccgtcattc
    15541 ccgcgcaggc gggaatctag aaactcaaag ttgcaagaat ttatcggaaa tgactgaaac
    15601 ccaacgaacc tagattccca ctttcgtggg aatgacgata tatcagtttc tgcgccgaca
    15661 ggtctggatt cccgcctgcg cgggaatgac ggcaaagagg tagattttcc gcgcgacagc
    15721 ggatagaagg aaataaaaat ggaactgaaa tttgaagaac tgccctatca gcttgatgcg
    15781 gtaaatgccg ttgcgaatct ttttgcaggg cagccgaacc atgcccggac attcgatttg
    15841 accagtcagg ggacggggcg gtttgtcggc aacggcttgg atttggattg ggaaacgcta
    15901 ggcctgaatc tgaacacggt gcaaaagcaa aatggacagc ctgaaacgga aatcggcgcg
    15961 cacgggctga atttttctct agaaatggaa acgggtacgg gcaaaaccta tgtctatctg
    16021 cgcacgattt acgaattaaa ccgcagttac ggctggaaga aatttgttat tgtcgtaccg
    16081 agcgtaccga tacgcgaagg cgtgttgaaa agcctggaaa ttacgaaggc gcattttgac
    16141 ggagtgttcg gcaggcccgt gatgcgttat gcggaatatt caagcagccg gctatcggac
    16201 ttgcgcactt ttgcggtaaa cgaccacatc gagattttgg tgatcaatat ccagtcgttt
    16261 gaaaaagatg gaaacgttat caataaggta aacgaaagcg gggaaacgcc gattaagttt
    16321 attcaggaaa cgtgtcctat cgttattatc gacgagccgc agaatatgga aacggaaggc
    16381 cgtctgaacg ctttggaatc gctcaatccg ctctttaccc tgcgctattc ggcaacgcat
    16441 aaacaaccgt accacaaggt ttacagcctg aatcccgtgc aggcatacaa tttaaagctg
    16501 gtaaagcaaa tcgaagtttt gccggtattg gcggaaaacg atgtgaacgg cgcatttgtg
    16561 gagctgctgg aagtaaagca ggcggccaaa ggccgtccga aagccgattt gaacattcat
    16621 tatcaggata aaaaggaaac caagaagaaa aaggtatccg tcaaatggga agacgatttg
    16681 tttgaaaaat cgggcggtaa cgaagcttac cgccacggtt ttatcattga cggcatggat
    16741 ttcgataagc aggaattggc gttctcaagc ggtaaaacca ttacgcgcgg cggagacggc
    16801 gatgcggtgc gcgatgaaat tatgaaaaac caaatccgcg aaacggtaag ggagcatttg
    16861 gcgaaggaga aacggctgaa tccgctgggg attaaggtgt tgtctctgtt cttcatcgac
    16921 cgtgtggaga attaccgtac agaagacgga acggcaggca agtttgccct ttggtttgaa
    16981 gaaatctatc gggaacttgc aggcaatacc gatacggctg gcgtacacga cggctatttc
    17041 tcgcaagaca ggaaaggccg tctgaaaaac accgacggca cgacgcaggc ggacaacgat
    17101 acctaccgcc tcattatgcg cgacaaggaa accctgcttt cctttgacag cccgctgcgc
    17161 tttatctttt cccattccgc actgaaagaa ggctgggaca accccaacgt tttccaaatc
    17221 tgcacgctca acgaaacccg ttcgccgctg aaaaagcggc aggaaatcgg gcgcgggctg
    17281 cgtatcgccg tcaatcagga aggacggcgc gtgcgcgatg aaaacgtgaa catactgacc
    17341 gttgtgccga acgaaagcta cgaattgttt gccgccaatc tgcaaaagga atacgaagac
    17401 gaatgcggta ttaaatttgc cgccgaaaac atcaaaaacg gcaataagaa gaaaaaacag
    17461 ttattccgca aagatttccc gctcgacccc gaatttcagg caatttggga caagttgaaa
    17521 tacaaaacgc gctatcgcgt gcgcttcgat acggaagaat tgatcaaaca ggcttcggaa
    17581 gcggtggcaa acctgccgga aatccaaaaa ccgaaaatcc gcatccgcaa ggcgcaatta
    17641 aaacaatcac aaacctacgg tatcgaaaca gttgaaattg ccggccgcag ccgcgaaatg
    17701 gacgttcaat gggagattcc ggacatactc ggcgagattc aaagaaaaac ggggctgacg
    17761 cggcgcacgg tgtacgaaat catcatacag tcaggccgtc tgaaagatgt agccggtaat
    17821 ccgcaacgct tcatcgacct taccgccgaa gcggtaaacc gcagcctgca cgcgttgatg
    17881 gcggcaggaa tcgagtatga gagaaccggc gaagtgtacg accaaaagct gttggaagat
    17941 atggcgcgcg aaggtatcga gttttacgaa aacggtttta cattcacagt aagcaatccg
    18001 caaaaaacca tttgcgaaaa ctacatccct ttggattccg gcacggaaaa agcctttgcc
    18061 caaaattgcg aggattatga tgcgaaaaat gaaggaggcg tggcgtttta cttcaaactg
    18121 cccggctggt tcaaaatccc gaccccgctg ggcaattaca atcccgattg ggcggtggta
    18181 aaacaggttc gggataaagt gtatttcgtt gcggaaacca aaaacaccgg caaaggcatt
    18241 cagaccggcg tggatatgga caagctgcgc caaaatgaga ggctgaaaat caaatgcggc
    18301 aagcggcatt ttgaagcgct gggcggtttg gcataccgtg ttgtggaaaa agtgggggat
    18361 ttaaagtcgg aaaaagagga gcaggcttaa aacaaacagg tgtaatcgta gtatggcgat
    18421 gttggattgg ggcggcggca accgaagatg cggatatggt aatggctttg attgggtaaa
    18481 ccgatcgggg actgaagatt gtttcagacg gtctgttgat tttcttattt cggaaatggg
    18541 aagatggaag gctattgccc tgtcagacgc acccaaagcc tttgccaccg gcttcttttt
    18601 tctacatttt ccagtgcgac gatttctttt tcggcaatgg tgtatccgtt ttgtctgatt
    18661 ttgatttttc ctaaaatttg cccttttttt accggggcgg gaatcggctg tatggtttcc
    18721 aaaatctgtt ctgccatttt cgcttcctta tgtggcagag tgatgtaggc ttctttgagg
    18781 aagcctgcgc ggacggtttt tttgctgcct ccggaaattt ggatttgggc aacggttttg
    18841 cctttcggat atattttggg cgtatcgaag gcttgcaatg cccagttcag cagcttgctg
    18901 ttgtctgagg cgcgtgtttc cgccgattcc gaacctaatg tgatgacaag gatgtgcctg
    18961 ccgttgccgg agtatgacac ggcaaggttg tagccgccgc tttctgtgtg tccggctttc
    19021 agaccgttta cattgttgtc cctatataaa aggatattgc ggttgttttg ctctatattt
    19081 ttgaatttga aagatttgat ggaaaacagc gggtaatatt ccggaaagtc gcgcatcaat
    19141 gcttcagaca gctgggcgag gtctttggcg gtggaaacct gtccttctct actcaagcct
    19201 gtcgggtttt tgaatacagt gttcttcatg cccaagcgtc gggcttcttt gttcatttgt
    19261 tgcacaaaat tttcaatcga gccgttgccc agccggccgg caagggttag ggcggcatcg
    19321 tttgcggata gtgcaatcat gccttttaag agtttgtcgg tgctgaccgt atcgccggga
    19381 cgtacaaaca ttctgcttcc ttctgaagcc catgcggatt cgggtatttt taagttttct
    19441 tcagattgga tatttcccga tttcatgttt ttgaaaacca aatatgcggt catcagttgg
    19501 gttagtgccg ccggttcgac aggggtattg atgtttttgg cggataaagt ctgtctgctt
    19561 tgaaggtcga taacgatgtg tgccgctgtg agggtttcgg gtgtttggag cgtgggggcg
    19621 gcgtgtaccg tcagtttgtt gggcgcgggc gatgcagccg ttgtgtgaga aacgcctaag
    19681 atgatggaaa gcaggacggg caggatttta tgtgctgtca tgaaatattc taattgtgtg
    19741 cgtgtttcag tctgccgatt atacgcttag ggtgtctgat cgggcggatt tttcttgctt
    19801 tcgcgccgtc ttgggcgtat ggttttgggt tttgcgattt taataaaccg attatcccat
    19861 attgaattat gaacacgccc cttccttatt ccaattacct catccgcatc ctgacggcat
    19921 ctgtctatga tgtcgcggtc gaaacgcctt tggaaccggc acgcagcctt tctgtacgtt
    19981 tgaaaaacaa catccttttg aaacgcgaag atttgcagcc ggttttttcg ttcaaaatac
    20041 gcggcgcgta caacaaaatg tccaagttgc cgaaagatgc gctcgcttgc ggcgtgattg
    20101 cggcaagcgc gggcaatcat gctcaaggcg tggcattgtc cgcacagcgt ttgggctgcc
    20161 gtgccgttat cgttatgccg gagactacgc cgaaaatcaa agtggatgcg gttaagagcc
    20221 atggcggcga ggtggttttg cggggcgttt catacaacga tgcctacgat tatgcgatgg
    20281 agttggcgga aaaagaaggg ttaacctata tcgcgccgtt tgatgatcct gatgtgattg
    20341 cgggacaggg gacggtgggg atggaaattg tcagccagca tcccgatcca atccgcgccg
    20401 tattcgtacc gataggcggt ggcggtttgg cggcgggcgt ggcggcattt atcaagcagg
    20461 tccgtcccga aatcaaagtt atcggcgttc agaccaacga ttcctgctgt atgaagcaat
    20521 cggttgaagc gggcgaaatc gtccatttga aagatgtcgg gctgttttca gacggcacgg
    20581 cggtcaaagt cgtcggaaac gaaaccttcc gcctctgcaa agaacttttg gatgaaatca
    20641 ttacagtcga taccgatgcg gtttgcggcg cggtcaagga tatttttgat gacacgcgca
    20701 gcattaccga gccggccggc gcgttggcgt tggcaggtct gaaagcctat attgcccgag
    20761 aaggcgcgga aaaccaaacc ctgattgccg ttaccagcgg cgcgaatatg aattttcacc
    20821 gtttgcgcca cgtttcggaa cggagcgaat tgggcgaggg caacgaaggt atttttgccg
    20881 ttaccatccc cgaagaacgc ggcagcttcc ttaagtttgt caatatattg ggaaatagga
    20941 atattaccga gttcaactac cgctacgggg acgatgaaaa agcgcatatc tttgtcggac
    21001 ttcaagcggc aggcccgcag gatttggcgg ttatcggcag ccggttggat gaggcgggat
    21061 tgcccaatgt cgacctgacc gatgatgaga ttgccaaaat ccatatccgc tatatggtcg
    21121 gagggcggac ggacaaagta gaaaacgagc gtttggtcag ttttgagttt ccagagcgtc
    21181 cgggggcgtt ggcgcgtttt ttgaaccata tgcagggagg gtggaacatt acgctcttcc
    21241 attaccgcaa ccacggtgcg gattacgggc ggattttggt cggtatcgat gtgccgccgc
    21301 acgatgccgc cgcatttgac ggtttcttgg aaagtctggg atacagctat cacgaggaaa
    21361 cgcaaaatgc tgcgtacaag ctgtttcttg cctgacgctt gaaagtacaa tgccgtctga
    21421 aagcctttca gacggcattg cgctttcatg gttaaatcga atattcaatc agctcgtttt
    21481 gagaaaagac ataaacctgt ttcggaatca gcttcaattc tttgccttcg gcgattgggt
    21541 aacgcgcggc atcgctgcca gccagcgtga tgtgcacgtc ttgtttgccg tgtttcacca
    21601 aaatatgcgt caatgcgccg acggcgtgga ttttttcgat ttcggcacaa atcatcgggg
    21661 tttcgttttc ggcggcaagc tgccattcgt gcgggcggat atagccggtg gcggtttgtt
    21721 cctgccattt gtattgtgcg tccaatttcc acgcgaagcc gttataatgc cagaagcctt
    21781 tttcgatgcg tccttcaaaa gcgtgggttt cgccgaggaa ctcggtaacg aaggcatttt
    21841 cgggtttgcg gtaaatagct tcggcgctgc cggtttgttc gattttgccg tggttcatca
    21901 cgacgatttc gtcggaaact tcgagggctt cttcttggtc gtgcgtaacc agaatgctgg
    21961 ttacacccag gttgtgatgg atgtcgcgca gccaggtgcg taattctttg cgtactttgg
    22021 catccaacgc gccgaagggt tcgtccaaaa gcaagagttt gggttcgacc gcaagcgcgc
    22081 gggcgagggc gatgcgctgg cgttgcccgc cggagagttg gtgcggatag gattttgcca
    22141 aatgagagag ctgcacgagc ttgagtaatt cttcgacttt ggcgcggatt tgtcctttgg
    22201 acgggcgttc ggacttgggc aatacggtca aaccgaaagc gacgttgtca aacacgttca
    22261 tgtggcggaa gagggcgtag tgttggaaca cgaagccgac tttgcgctcg cgtacatgct
    22321 tggcggttac gtcttgcccg tcaaagagaa tattgccgcc gtcggcgttt tccagtccgg
    22381 cgataatgcg taaaagtgta gttttgccgc agcctgaagg gccgagtagg gaaacgagtt
    22441 tgccggtggg gacgttgagg ttgatgtttt tcagcgcgtg gaacgctccg aagtgtttat
    22501 ttagattttg aatagtaatg ctcatgctgc gttcctttcg gcggcggcga gttttttgtc
    22561 ttgtaatttg gtgatgatgt tctgcaccgc cagcgtcgcc agtgctaaaa gtgccaatac
    22621 gccggagagg gcgaatgcgc cggtgaagtt gtattcgttg tagaagattt cgaccaaaag
    22681 cgggacggtg ttggtttcgc cgcgtatgtg tcccgatacc acgctgaccg cgccgaactc
    22741 gcccatcgcg cgggcgttgg taaggatgat gccgtagagt aacgcccatt tgatgttggg
    22801 cagggtaacg cgccaaaaca tctgccagcc gcttgcgccg agtatcaatg ccgcctgttc
    22861 ttcgctgtcg ccctgtgcct gcatcagcgg gatgatttcg cgtgcgacaa aagggaaggt
    22921 aacgaacagc gtcgccaaaa taatgccggg gatggcgaaa ataatctgta tgccttgcgc
    22981 ttcgagccgg ccacccaatg ccgtgtgcgc gccgaacaat aagacgaaca ttaaaccggc
    23041 caccacgggc gatacggaaa acggcaaatc gagcagggtg gtcagcaact gcttgccgcg
    23101 aaaatcaaaa cgggtcagca gccacgccat cgccacgccc aagacggcat tgacggggac
    23161 gacaatcagc gcggtaatca gcgtcaattt gatggcagac cacgcttcgg gatcgtttaa
    23221 ggatttcagg tacaaatccc aaccgccttt taaggcttcg taaaacacgg cgacgagcgg
    23281 cacgaccagc atcagcagca gaaagcccag cgcggcggca atcagcaaca cgcgcagccg
    23341 gcgcggttcg gtcaggttgg gattggcgga atagggtttc atggcggtgg tgttccccgg
    23401 ttggttgttt taaggtgttc ggatgccgtc tgaaaaaccg ttttttgtgg ggcggattcg
    23461 ttttcagaca accttttatt gattaaggga ataaaaacgt ctagctaccg tcattcccgc
    23521 gcaggcggga atccaccgca gggcaacagg aaaacagaaa ataaataagg cagctgaaat
    23581 tcaccaatgg attcctgcct gcgcgggaat gacggtaaca ggtatttcag acgacctcaa
    23641 cccttcgcgc ccgaacgcct gcccaacgcc cactgcatca cgttcagcgc aaacagaatc
    23701 acaaacgaaa ccagcagcat aaacaacgcc accgccgacg cgccctgtac gtcgaactgt
    23761 tccagcttgc ccgtaataat cagcggcagg atttcagaaa ccatcggaat attgcccgcg
    23821 ataaaaatca ccgagccgta ttcccccgtt gcccgcgcga acatcattcc cgcgccggtc
    23881 aagagtgccg gcgtgatttc cggcagcagc acgcggcgga acgtcgtaaa gcggtttgcg
    23941 cccaaagtcg ccgccgcttc ctcatattcg cccgataatt cctccaacac cggctgcacg
    24001 gcgcggacga taaagggcag gctgacgacg accagcgcaa tccaaatgcc gacgggtgta
    24061 aacgcgattt tgatgcccaa aggctcgaaa aaacggccta tccaaccgtt gggcgcatac
    24121 agggtcgcca acgcgatacc cgtaaccgcc gtcggcagcg caaacggcaa atcgaccagc
    24181 gcgttcgcca gacccttgcc cgggaattca taacgcacca atacccacgc caccagcgtg
    24241 ccgaacacga cattggtcag catcgcataa aacgacatcc gcaaactcag ccagacggcc
    24301 gccaagacat tgggttcggt aatcgtgttc caaaatccgc tccagccgat ttccgccgcc
    24361 ttcgccgcca tcatcgcaaa cggcaagacc acaagcagcg acaggcacaa tacggtcaga
    24421 ccaaggctga gtttgaagcc gggcagtacg ccgggcgttt tgagcgctaa cataaaacaa
    24481 tgctgaaaat aaggaaaagg aaggactact ttaacgatgc cgtccgaaaa acggaaagaa
    24541 tggaaagttt ggtgcaaaga cgaatttgtt ataaagcggt tggcagtttc tcaagcgggc
    24601 gcgatgtttt aaaatatagt ggattaaatt taaatcagga taaggcgacg aagccgcaga
    24661 cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat
    24721 aacgccttgt tttgacggaa aaccatcata taaaggaaca cttatgcaga ttttatcttt
    24781 tcagccggac attgcggaac gtatgctgga aggtacggaa ggcgagtcgg tcaacgaaaa
    24841 cgcacaattc gtccgtacgg acaacggcta ttggattgcg tggcatgaag gcgtagcggc
    24901 actgcttgcg cccgatacgc cgccgggcat tccctgtttt tgggtggaag gcgcggaaag
    24961 ccttgaagag ttgtgcgtca tggtggaacg cggcgagttt gacgaagtgg aagagtttga
    25021 cggcgatgac gacgaatggc tcgaaacggc acagggttgc gggcaccacg gcgacgcttg
    25081 cgcctgcgga cattaaaggc attgcaggct tgccgcaagg ggggcaaggc tttgccgttt
    25141 tttaaataat cggtggattg catctgtttg caatgggctg aacgcttccc tttgtatttt
    25201 attgaataaa aaaacttatc ttggtattat aattaaggca gcatcaatta ttttgggatg
    25261 acaataaacg caaagcattg atttgcgccg attgcagact tattatagca ggttgcggcg
    25321 cggacttaat gggaattgtt tatagagcat gatggtctga aacatcatta attaaaaata
    25381 ccattaatcc gatttatatt tatttcaatt tcaatggaaa aacatcaatg acaatgattt
    25441 taagcatttt aagcctgttt tttatcatca gactgttatt tttagccgtc tctattaaac
    25501 atgaaaaagc cttgattgcc aaaggggcga aacaatacgg aaaaaccaat tccacgctgc
    25561 ttgcggcagt tcatacgctt tattatttgg cgtgttttgt ttgggtatgg ctttctgaca
    25621 ctgcttttaa tggcatatcc ttgattggta cgctgacggt gatggcttca tttgtgatat
    25681 tgtcattgat tattaagcag ttgggggaga tttggacggt taaaatctat attttaccaa
    25741 atcatcaaat taatcgttcg tggttgttta aaacattccg ccaccccaat tattttttaa
    25801 acatcatacc cgaactgatt ggcatcgcct tattatgtca agcgtggtat gttttattga
    25861 ttggtctgcc catttatttg ctggtcttat ttaagcgtat ccgacaagaa gaacaggcga
    25921 tggcaacact tttttaaccc gtttcatcaa ttatagtgga ttaacaaaaa ccagtacggc
    25981 gttgcctcgc cttgccgtac tggtttttgt taatccacta tattccgcca tctctaagat
    26041 ttacagcgat acacgggtga tttaaggaat gcccgaaccg tcattcccgc cacttt
//
LOCUS       DAWFKN010000021        26001 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFKN010000021 DAWFKN010000000
VERSION     DAWFKN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.021"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..293)
                     /locus_tag="WGR46_000887"
     CDS             complement(<1..293)
                     /locus_tag="WGR46_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0855857.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELS"
     repeat_region   733..1758
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=799..834
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(1822..2148)
                     /gene="cas2"
                     /locus_tag="WGR46_000888"
     CDS             complement(1822..2148)
                     /gene="cas2"
                     /locus_tag="WGR46_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0855858.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2141..3055)
                     /gene="cas1"
                     /locus_tag="WGR46_000889"
     CDS             complement(2141..3055)
                     /gene="cas1"
                     /locus_tag="WGR46_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0855859.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(3122..6370)
                     /gene="cas9"
                     /locus_tag="WGR46_000890"
     CDS             complement(3122..6370)
                     /gene="cas9"
                     /locus_tag="WGR46_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0855860.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            complement(6648..10082)
                     /gene="dnaE"
                     /locus_tag="WGR46_000891"
     CDS             complement(6648..10082)
                     /gene="dnaE"
                     /locus_tag="WGR46_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0855861.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVRIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLKNGDNSGLICLSGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILSPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITSARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGTFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10366..10602
                     /locus_tag="WGR46_000892"
     CDS             10366..10602
                     /locus_tag="WGR46_000892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855862.1"
                     /translation="MADADDQRIGQFVFEDVHQRQCGFVGEQDLQPAVGQDFFERIAA
                     YGVSDGIFTFTEQAGRDVPPVLRQFWVRRRQVLQ"
     gene            10547..10861
                     /locus_tag="WGR46_000893"
     CDS             10547..10861
                     /locus_tag="WGR46_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0855863.1"
                     /translation="MCRRFCGNFGYGGGRCYNNGLFLKGDIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EA"
     gene            10839..11048
                     /locus_tag="WGR46_000894"
     CDS             10839..11048
                     /locus_tag="WGR46_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855864.1"
                     /translation="MDTILKHKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            11341..12177
                     /locus_tag="WGR46_000895"
     CDS             11341..12177
                     /locus_tag="WGR46_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0855865.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
     gene            complement(12229..13521)
                     /locus_tag="WGR46_000896"
     CDS             complement(12229..13521)
                     /locus_tag="WGR46_000896"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0855866.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDTALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13577..15487)
                     /gene="pglD"
                     /locus_tag="WGR46_000897"
     CDS             complement(13577..15487)
                     /gene="pglD"
                     /locus_tag="WGR46_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0855867.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVICGLAVYRPDDIAALIGRCGVKKILLAIPGATQAQ
                     RRKIIGKLEAYPCEVLTIPGIKDLMDGKLSIGTLKKISVSDLLGRDPVTPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPNITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDHIRSACDRYDQQAIRTLLINALTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15535..16710)
                     /locus_tag="WGR46_000898"
     CDS             complement(15535..16710)
                     /locus_tag="WGR46_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0855868.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            complement(16833..16946)
                     /locus_tag="WGR46_000899"
                     /pseudo
     CDS             complement(16833..16946)
                     /locus_tag="WGR46_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17182..17868)
                     /locus_tag="WGR46_000900"
     CDS             complement(17182..17868)
                     /locus_tag="WGR46_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0855869.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
     gene            complement(17855..19432)
                     /locus_tag="WGR46_000901"
     CDS             complement(17855..19432)
                     /locus_tag="WGR46_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0855870.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TVQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(19425..20592)
                     /locus_tag="WGR46_000902"
                     /pseudo
     CDS             complement(19425..20592)
                     /locus_tag="WGR46_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(20612..21127)
                     /locus_tag="WGR46_000903"
     CDS             complement(20612..21127)
                     /locus_tag="WGR46_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0855871.1"
                     /translation="MDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDNLKAQAER
                     LGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYDTPVVTYD
                     MAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVAALCSDDE
                     IYRTTMAAYTM"
     gene            complement(21075..21686)
                     /locus_tag="WGR46_000904"
                     /pseudo
     CDS             complement(21075..21686)
                     /locus_tag="WGR46_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21683..23104)
                     /locus_tag="WGR46_000905"
     CDS             complement(21683..23104)
                     /locus_tag="WGR46_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0855872.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLSVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(23299..24408)
                     /gene="ribD"
                     /locus_tag="WGR46_000906"
     CDS             complement(23299..24408)
                     /gene="ribD"
                     /locus_tag="WGR46_000906"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0855873.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGFAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            complement(24440..24904)
                     /gene="nrdR"
                     /locus_tag="WGR46_000907"
     CDS             complement(24440..24904)
                     /gene="nrdR"
                     /locus_tag="WGR46_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0855874.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPSVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            complement(24933..25775)
                     /locus_tag="WGR46_000908"
     CDS             complement(24933..25775)
                     /locus_tag="WGR46_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0855875.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         5416 a         6374 c         7631 g         6580 t
ORIGIN      
        1 ctgctcagtt cgtcgaaacg gcaaaacagg ttgaaatcga tgcgggtaat gaggctgtgt
       61 tcgagttcgg gatcggagag gctgtgccat tgtccgagca gaacggcttt gaacatggac
      121 aacagcgggt aggcgggacg gccgcggtgg tctcgaaggt aacgggttct ttgacggttc
      181 aggtattgtt cgatcggttg ccaatcaatc acctggtcca acttcaatag cgggaaacgg
      241 tcgatgtgtc tggcaatcat ggcttgggcg gtttgttgaa agaaggtgct catgagaaat
      301 cccctaaatg tcttggtggg aatttagggg attttgggga attttgcaaa ggtctcattt
      361 tgcaagattt tagtttaatc atcaaacagg gaacagcaaa aatccccgct gcaacgggga
      421 tttttgttcg gctctcgcct gtgtgattaa cttcactagg agaagctaaa atgcttaaat
      481 tccttatgcg tgggaaaaag ttgctgctgt ccgttgaagt gaaccacaag ttcattttag
      541 cggttgcttt gctgttaagt caatgcagta gctaacccga ccacaatcaa aaaaacgcaa
      601 gttttccgcc tactctgtga gtgggcggtt ttttcttatt tcaaatctgt gacacaatat
      661 ctgtcgcctt tcttgccata attgcactgt gtctcaatga ctttaacgca cgttcgctta
      721 tcgcaacggc tggttgtagc gcccattctc atttcgcagt gctacaattt tttgtatcca
      781 ttcccagccg gaaattaagt tgtagctccc tttctcattt cgcagtgcta caatctttaa
      841 ctttgaccgt gtgcaatcca gttagttgta gctccctttc tcatttcgca gtgctacaat
      901 ctgaccacac ctatccgcca gtgcttatgc gttgtagctc cctttctcat ttcgcagtgc
      961 tacaataagc cggtttatgg caattctgcc gcgtccgttg tagctccctt tctcatttcg
     1021 cagtgctaca atatgccttt ttttgtactg ttgtttgaac gagttgtagc tccctttctc
     1081 atttcgcagt gctacaataa attcgtttca gatagcaaac gcagtagtgt tgtagctccc
     1141 tttctcattt cgcagtgcta caatgccgga tatgacggtg ggcaactggt acaggttgta
     1201 gctccctttc tcatttcgca gtgctacaat atttcgcttt tgtattgatt caaggtgctt
     1261 gttgtagctc cctttctcat ttcgcagtgc tacaataaat tcgtttcaga tagcaaacgc
     1321 ggtggtgttg tagctccctt tctcatttcg cagtgctaca atagtattcg tcgatgatgg
     1381 aaactcgagc atgttgtagc tccctttctc atttcgcagt gctacaattt tctgttcttc
     1441 gtggatatac tggaatttgt tgtagctccc tttctcattt cgcagtgcta caatcttccg
     1501 cgcttccggg ggttaagtcg ggacgttgta gctccctttc tcatttcgca gtgctacaat
     1561 cttcatctct gcaaccatgt tgtccagcat gttgtagctc cctttctcat ttcgcagtgc
     1621 tacaataaac gcggcttttc ggggttttta ccttgggttg tagctccctt tctcatttcg
     1681 cagtgctaca atcttccgcg cttccgggtg tgaggtcggg acgttgtagc tccctttctc
     1741 atttcgcagt gctacaatac ttcgacggga aatccttatt tcataagggt ttcccgtttt
     1801 atttgttatg aaaaaatggg cttaaaataa caataattgg tctgaattga cctttttttc
     1861 ttgggttttc agctcgccca acagcagttt catggcggca tattgctttt ctgttatctc
     1921 caaacagcgg attgagcctt cttgcggaag gtttgcacac agtctgttgt ggtgtttttg
     1981 caacgaatcg cggcctttga cgatacggct gtatacggaa agttgcagca tttggtatcc
     2041 gtcttttaat aaaaactggc ggaattgatt ggcggctttg cgctttgccg ccgtgataac
     2101 cggtaggtcg aagaagacga taatcctcat aaatttggcc tcactcatat tggtattctt
     2161 tcaatggtag gatttcaggc agtttcagtt gtttggcgtt tttatcggtt acgccggctt
     2221 gaaatgacga aatcattttg tcgatggcag ccaaggtgct gaaatgttgc cgttcgatgc
     2281 tggtttgata gtagaggatt ttgatgaggt gttgtttgat gccgagcgtc agttcagttt
     2341 tcagacggcc ttgttcgtac aaatgtatca cggtcaggtc ggccaatggg cgtaagggtt
     2401 cgataaagtc atcggcgaga ttaaatggat tgagttcact tctatgaaac aagcctaatg
     2461 caggtaacca accgtataag gtcagggcgc gtgcaactgc ggcacggagt acggcgtagg
     2521 tgtagttcaa tgcggcattg acggcgttgt tgtcattacg ggtaaatttt tcgccaaaga
     2581 gggcttgaaa gtagagggcg gcagcttggg cttcacggtt tcctgtatcg cctgaacgga
     2641 cttcagatgc caaagtgcgc agccgtttgg ctgcaagatc attgcctgtt tcgtctgcga
     2701 caaatgcttg gttcaggatt ttttggcgga cgatatgttg ccagagttgc ttttttaagg
     2761 gttcgcttat gttcagttgc agtttgagga ttttaagttg gcggtgatat tgggcataag
     2821 gcagccattg tccgcagggc aggaactgct cgtcgcaggt cagcagggtt gcaccatgct
     2881 cggctaaggc tgataggagc ggggcggtaa tcagggtttc gcgattttcg atgatgataa
     2941 cggcgatgtc ctctaatggt acggtgtggg actcgccgtt ttgttgaatc agcagttgcc
     3001 ggcgttgcaa ggatagtttg ccgccgtttt gaataagcag gctgcgccaa gtcatgtttt
     3061 tccccttatt tttatataaa aaatccctgc ttcaggccgc ctgaaacagg gatagattaa
     3121 tttaacggac aggcgggcgt tttttcagac ggcatggtct gatttctttg cccagttcgt
     3181 caatttggta tttttggaat gaaagggcgg ttttgacgcc gataccttcc agtattccat
     3241 ttttgccaat tttatgatca agatcatgaa tgcgtatatt gatattaccg gtgcctcgat
     3301 ggcagctggc aaagtaacca aacattctag ctttttttgt tataacctcg actaaatcat
     3361 tagggtgtaa tgagaattta aagttgaaac tatcatcaat aagttgccaa tcttcttcat
     3421 cttttccttg tacaacagcc ctatccggca aaatcccttt cgctacctgc caactgtaaa
     3481 tcggtaccag ataatacttg tcgcctttct caaacacatc tacgcgcacc atggttgcgt
     3541 tgtcggcaat accgttatgg ttgcgcaccc atacgccggt tttctgtact tgctctacgc
     3601 gtacggcttt tacctgttgg gtgcggttgc ctgctttatc gtatttgtaa aacggctcgg
     3661 caaaggcttt ggcaggatcg tctttatgtg cttccagccg tgctttcagt gcttcgtata
     3721 gcttaggttc gcgctcccga ttgaccattt tttccaagtc tttcagtttt aactgtgtta
     3781 acggtacgcg caacacgctg acgccttcgt ccagtcgttt ggcggatttg acggtctcca
     3841 tatgcccttg cccgctcatc ttccgattgg gcgcgcgtga aacaaacagt ggcgtaacgt
     3901 attcgtgtac ggcttcgggg cgagatgata atttttcggc aagcaacgtg cgcagttttt
     3961 ctggggtatc ggcttcttcg aattcgggtt tgccgtccgg tttgccgaag acgcgaatca
     4021 tgacttcttg tgcgaaaaat tcccaaggtt gtgggaagtg tgttttttga tgcagcactt
     4081 ctcctgtttc tttgtctatg gttttaccgt caaacgcgtt catctcttta tagcgtacaa
     4141 aacgggtaat tttctgctgc atggcaacgg tcgagcaggc aacgacgacg gcgtccaagg
     4201 cgtgatggcg gtcgttttcc gcacgcactt tgcgcaatcc ccaaaagccg cgcaacagat
     4261 tggtaatttg tccgttggat gcaaagacac gtttcttgcc tttacctgtc agccgcatac
     4321 ggtcggcaac aaattgacac aggaaacggt tgacgtagcg cgtgtcgttc agattgcgtt
     4381 ctttaaagcc gtcttcatcg aatttttgca gcagaatccg ttgtttttta ctgcgcggga
     4441 aacggctggt ttcgacacgc gctttaaatt cctgccattc gcggctgttg tctttgccgt
     4501 tgaagtattc gtaaggggtt tgattgcctt tgttttggtt ttcgctgccc aataccagta
     4561 ctttattgtt gaaactgtcg tcccatgtgc gcgagaacgg cagggcatgg tcgatttcga
     4621 catagccttt ttcgttcaga cggcctaagt tgatttcttt gcccgaatac aggcatttgc
     4681 cgtgttgttg ctcgtacaga cgcagtttca gaatatcttt ggatttgggt tctccgacaa
     4741 aattggggaa atactctcgg aatttggcgg cggctttttc cctgtctttg cggttttctt
     4801 cttggcgttt ctcaatttct ttgcggtctt taaacgattt acctacttcc cttgcagttt
     4861 caatatggat acgagctggg gagccgtaac ggcgtaccac gccgttaatg accttacgtg
     4921 cttgagataa ggcgcgcaag acgacggggt tgcggatttc gtcggcggga atcggcggca
     4981 gataaatctt ttcttccgta ttcttcttgc cgtaatggtc tccgtagatt tcggcgcagg
     5041 cttcatcgta acgtttgcct tgttccatta gaggcacaat tcggcgcaat gctttcaagg
     5101 aaatttggac gaacttatcg aagctgatgt gtttcaacag cgcttctaag atttcgggct
     5161 gtatacggtc tttcagacgg cctgtaatgt cttcatcggt tttgaacagg gagaatgccg
     5221 tgccgatttc gtcttgtaat tcgggagaaa ggtttaatgg ggattttttg tctttcaatc
     5281 cttctttttc cagtgcacgg ctgatggcat ggtaggcctt catttccatc aatgttgagg
     5341 cttcggcatt gtctttacca tagcgcaagc ctttgaaaaa ggcggtatct tctaaaccca
     5401 gcagcttacg ggcttgtgcg taagtcagtt tggattttct gtatggctcg tccataagcg
     5461 tggcgcgttc ggtatcggtc aatggccgct cgctgccttg ctctaaaata cgcaggttgt
     5521 tcagcttggt cagccagatg aaacgttcgg ctgtgtaggt gtttttagcg gctttcggct
     5581 ctgccggttc gaaggtgcaa tgccccaaca ttttttgaac ggcatcgccg gacagggcag
     5641 ggcgttgcgt catcagtagg gtttcaatac cttctttaag gccgcctgaa acatgcggat
     5701 tgccaaattc tttttgtttt tcaaacagca aaatcagctc cgcctgtaaa tctttgcggc
     5761 tgaacgtatg cgaataatcg ccgcgctgat tgcggatatg gccgctttct ttctcaaatt
     5821 tatttaaagc caattcggcc ggtgtgcgga aatcgcctgt ctgtaaggca tgggcattgt
     5881 cggctacgcc tttgagcaaa gcgccaagct ctttatcggc agtttcgccc tcgttttttc
     5941 tttgcgacaa ataaccgcgg tgtttgatta aatgcaacaa gactgccgac cactctaaag
     6001 gcgtcagttt gcggtctaat gcggctgcgc gaagttgcca tggtgtattc ggtaaggatt
     6061 tgatcaagcc gttttcgtca aaatcagcgg cttgtaatac gccttcgcgt ttcaataggc
     6121 ggcgggcccg aagcaggcga tgggcgcgac ggcgggtcag gcggcggaca ctgcgcgcca
     6181 aacgccttgc catggcaagg gagtcgcctg ttttcggtac ttcggcacgc tcaaatacgc
     6241 gcacgcccaa atcaatcagg cagattgggt tttcatcttc gtcaatttct accatcgccc
     6301 agccgacgga tgcgatgccg atatcgaggc cgaggatgta gttgattgga ttaggtttga
     6361 aggcagccat ttattactcc gtaaacaacg ttgcaaataa tgataatata tcttattgtc
     6421 ttatttatat acaaatagat tattgactta tcatctcaca acggctagaa tcccaaacat
     6481 attgtcgcac tgcgaaatga gaaccgttgc tacaataagg ccgtctgaaa agatgtgccg
     6541 caacgctctg ccccttaaag cttctgcttt aaggggcatc gtttatttcg gttaaaaaat
     6601 gccgtctgaa accgattttg ggcttcagac ggcatttata ttttgggtta ccagttgacg
     6661 cgcaccgacc ggctgccgag caatgtttcc agttcgccga acaatgcgga gctcggtgta
     6721 accgtccatt tcggcggcac ttgaagcctg cccgacgctt tttcgttggc atacgacagt
     6781 tgcagcggga tgcgcggcgt gtcgggcagt tggtgggcgg caagcagttg taccagttcg
     6841 ccgatgtcgt gatgcggggc gagggcgagg ctgaggctgc gggcgtagcg ttcgcgtgcc
     6901 gtttgcaggg tcatgacttg gtttgccatg atgcgcagtc cgtcgccgcc gccgtagtcg
     6961 tcgcggctga ctttggattc gataatcagc acttggtcgg ctttgaggca gtcggcgcag
     7021 ttttccagcg tctgaccgcc gaccatgatt tcaacctgcc cgctcaaatc ttcaaggctg
     7081 acgaaggcga ttttgccgcg cttgcccatc atcgtacgca cggcggtaac gaatccggcg
     7141 aggcgcacgc tgtcttgcgg cttcagacgg cctaatttgg tcggtgcgat ttggcggact
     7201 tcttgggcat acgggccgaa cgggtggccg gagaggtaaa agccgatgac ggttttttct
     7261 tcggcgagtt tttccgattc gctccacatc ggcgcgtcga tgagccgcac cggttcgatg
     7321 gcgtcttcca tcatgtcgaa aagtccgccc tgattggtgt tggcggcttt ttggtcggcg
     7381 ttgttcatgg cgaggtcgat gtttgccaag agcatggcgc ggttgggttc gatgctgtcg
     7441 aacgtgccgc cgcgtatcaa ggcttcgagg gtgcggcggt tcatgtgttc tttgccgacg
     7501 cgctcgcaga agtccaagag gccggtaaac ttgccgccgc tttgccgcgc ggaggtgatg
     7561 gattcgacgg cggcttcgcc cgtgccttta atcgcgccga gcgcgtagcg gattttcatg
     7621 tccggatacg gtgtgaagcg gtagtcggat tcgttgatgt cgggcggcag gaactcgata
     7681 ccgttggcgc ggcagtcgtc gtagaaatgc ttgagctggt ctgtgttgtc caattcagac
     7741 gacatggttg ctgccataaa ttcggcgggg taatgggctt tgagccatgc ggtctggtag
     7801 gaaatcaggg cgtaggcggc ggcgtgggat ttgttgaaac cgtagccggc gaatttttcc
     7861 atgtagttga agatttcgtc ggatttttcg cgtgaaatgc cttgttttgc cgcgccttcg
     7921 gcaaagattt cgcggtgttt caccatttct tcgggctttt tcttacccat ggcgcggcgc
     7981 aacaagtccg cgccgccgag cgagtagccg ccgataatct gcgccgcctg cattacttgt
     8041 tcctgataca ccataatgcc gtaggtcggt gagaggatgc cttccaacag cggatggatg
     8101 tattggaatt cctgtccctt catacgcgcg acgaagtcgg gaatattgtc catcgggcca
     8161 ggacggtaga gcgacacgaa ggcgatgagt tcttcaaact tggtcgtatg cgccgttttc
     8221 agcattttct tcatgccggt cgactcgaat tggaagacgg cggtggtgtt cgcatcgcgg
     8281 aagatttggt aggcggcctg gtcgtcgagc ggaattttgc cgacatcgac gatatcgccg
     8341 gtggtgtttt tgatgttgtt ctgcgccatt tcgataatgg tcaggttgcg cagacccaaa
     8401 aagtcgaatt tcaccaaacc cacgtcttcc acgtcgccct tgtcgtacat ggatacgggc
     8461 gaggcggatt cgtccgcctg atatacgggg ctgtaatcgg aaatcttgcc cggcgcaatc
     8521 aacacgccgc ccgcgtgcat acccaaaccg cgcgtcaggt cttccagctt tttcgccagc
     8581 gtaatcagtt cgtccgcttc ttccgcttcg attaattcct gaatctgtgg ctcggtctcc
     8641 atggcttttt ccaaactcag gggtttgttg gcttccaacg gaatcagctt ggacagtttg
     8701 tcgcacagca taaacggcag ttccagtacg cgccccacgt cgcgaatcac tgctttggac
     8761 gacatcgtgc cgaaggtaac aatctggctg actgcttccg caccgtattt ttcgcgtaca
     8821 tattcaatca cgcggccgcg gtttgcctga cagaagtcca cgtcgaagtc gggcatggaa
     8881 acgcgttcag ggttcaagaa acgttcgaac agcagcgcgt atttgagcgg gtcgaggtcg
     8941 gtaatcttca gcgaatacgc caccagcgag cctgcgcccg aaccacggcc cggccctact
     9001 gggcagccgt gcgttttcgc ccagttgata aagtcttgta cgataaggaa ataaccgggg
     9061 aatttcatct ggatgatgat gttcagctca aaatccaaac gttcctgata ttccggcatt
     9121 ttcgccgccc gctccgcctc gtcgggataa agctgaacca tgcgttcctg caaacccttg
     9181 tttgagagtt tgatgagata gtcatcgcgc gataaaccgt cgggcgtggg gaaaaggggc
     9241 aggaagtttt tgcccaatgt gatgtgcagg ttgcagcgtt tggcgatttc caccgtgttt
     9301 tccaaggctt cgggcaagtc ggcgaaacgc tccagcatcg tttccggcgg aatgaaaaac
     9361 tggcccggcg tgaaatcgcg cggacgtttt ttgtccgtca atacccagcc gcccgcgata
     9421 cacacccgcg cctcgtgcgc gttgaaatcg tcgcggttca taaactgcgt cggatgcgtc
     9481 gccaccaccg gcaaacccag ttcctccgcc agcttcacgc tgccggaaac gcaagcctcc
     9541 cattcggggc gttcgggcag gcgttgcagc tccatataga acgcatcggg gaaccacgcc
     9601 gcatacttca acgccgccgt acgcgccgcg tcttcattgc cgttcaacag attcacgccc
     9661 acttcgccgt aatgcgcgcc gctcaaacaa atcaagccgc tgttgtcgcc gtttttcagc
     9721 cattcgggat tgagttccgc gtgatggaca ttacggtctt tgccgacata ggccgccgtc
     9781 aaaagctcgc tcaagcgcag atagcccgca tcgttgcgga taatcagcat agcgcggaac
     9841 ggcttgtcgg gcgcattcgg attgcctatc cgcacgtccg ccgcgccgac gggcttaatc
     9901 cccgcgctgc ggcaggcttt atagaatttc accaaaccaa attcgttcat caaatcgctg
     9961 atgcccaaag caggcaaacc gtattcctgc gctttggcaa tcagtttttt aatccgcacc
    10021 ataccgtcgg taatcgaaaa ctcggtatgc aggcgcagcg ggatgtaggt cggctcggtc
    10081 atggcaaaat cggcgtgaac aataaaaggc gtattgtagc agggttgtct ttagatggcg
    10141 gtgtaggtaa tgctgtttca gattcagacg gcatgacctg caaatgtttt tgagctttta
    10201 cgacggcaaa aaaatgcctc ctgccgtatg gcggaggctt cccaaggagt attgatagat
    10261 ataaaggact atcaaactag ttataaggaa ctatatgcct tattcggacg gatggcaagc
    10321 agttaaatta attttacgtt caaacagatt tttgatttcg ttttgatggc tgatgccgat
    10381 gaccagcgta tcgggcagtt cgtgtttgag gatgtgcatc agcgtcaatg cggattcgtc
    10441 ggggaacagg atttgcagcc tgctgttggg caggatttct ttgaacggat tgcggcatat
    10501 ggggtttcag acggcatttt tacttttacg gaacaggcgg ggcgggatgt gccgccggtt
    10561 ttgcggcaat tttgggtacg gcggcggcag gtgctacaat aacggcctct ttctgaaagg
    10621 ggacatcatg gaagccactg tctatctcga agacaatgaa tacatcgcct tgtgcgacct
    10681 cttgaaattg gccggacttg ccgaaagcgg cgggcaggcg aaagcgttta ttgccgaagg
    10741 gctggtgttg cgcaacggcg aaaccgaaac ccgtaaaacc gccaaaatac gcggcggcga
    10801 agtcatcgag tttgacggcg cgcgcttgga aatcgccgat ggatacgatc ctgaagcata
    10861 aagccgaagc ccttttgggc gagccgcttt tagaagaacc cgtccgccct gagttgtggg
    10921 aatgctgcgg cagcgactgc ggcgaggcgt gcattcagac gatttactgg gcggataagg
    10981 cacgctacga cgcgcaacgg cggaagttga aagaagcggg ttgggcggaa gacgaaaatg
    11041 ccgtctgaaa ccagttcggc ttgataaaac cacgttttca gacagtcttt tatagtagat
    11101 taaaattgaa aacggtcata tcgccattcc ctcgaaagca ggaatccgga agtttgaaat
    11161 agcggttaac cttaaacatt tccaataaat caaagtctgg attcccgcct gcgcgggaat
    11221 gacggcatag cggcttttcc tttgcatttg ccatacactt gttttttgaa atcaccgccg
    11281 ccacaccgcc cgacaatatt gaaacaccct gcccaccatt taccacgcac ataacgcctt
    11341 atgatagacc tgcactgcca ttccaccgtt tccgacggta tgctctcccc cgccgaagtc
    11401 gtgcgcctcg cgcatcaaaa cggctgcacg ctgctggcgt tgaccgacca cgaccacacc
    11461 ggcggcatcg ccgaagcgcg tgccgaagcc gacaggctcg gtttgcgcct gattaacggc
    11521 gtggaaatct ccgtaacgtg gcgcgggcgt accatacacg ttgtcggctt agactttgac
    11581 gaacaagacg aaaacctgca aaacctgttg gcgcaagtgc ggcaaggccg tctgaaacgc
    11641 ctcgaagcca tcgccgccaa gctggaaaaa cgcggcattt cgggcgcata cgacggcgcg
    11701 ctcgcgctgg cggcaaacaa agaaatggtc agccgcacac acgtcgccga gttcctcatt
    11761 cgggtgggac acgtcaaaaa caagcagcag gcgttcacca aatacttggg cgacggcaaa
    11821 tcctgcgccg tccgccacga atgggcgacg ctggaagact gcgtctccgc cgtcaacggc
    11881 gcgggcgggt tggcggtcat cgcccatccg atgcgttatg atttgtccgc caccgccaag
    11941 cgcaatctgt ttgaagaatt taaaaaactc ggcggtgcgg gtatcgaagt ccacagcgga
    12001 aactgttgca aaaacgaccg cctcaactac gcgcttttgg cagaacgctt cggattgttg
    12061 gcaagcgcgg gcagcgactt ccaccgttta aacgacttca gcggcggcat tctcggcgcg
    12121 tgtcccgaac tgccggaaaa ctgcaaaccg gtgtggacgc acttttcccg acattgaatg
    12181 cggataaaaa tgccgtctga aaacttttca gacggcattt tttgcggttt aaacgttgtc
    12241 gtacagtttt cggacggttt tgccgatggc ggcaatgcct ttttccagcg tttgagcgtc
    12301 ctgcgcgatg ctcatgcgga tgcactcgcc cgcgtgcggg taatcctgcg tgtcgatgcc
    12361 gacgaagaaa tgttcgcccg gaataatcag cgtaccttcg gctttgagca tttcgtacag
    12421 ggtttgcgaa gaaacgggca ggttttcaaa ccagagccac aggaaaatcg cgccttcggg
    12481 tttgtggatt ttcatcgggt acgcgcccag ctcgcgcttg agcagcgaga cggcggtttg
    12541 cgcctgattg cggtaaaacg gccggatgac ttggtcggca agccgtttca tctcgccgct
    12601 ttccagtagc ggggcggcga tggccgcgcc gaagcgcgtg ggggcaaggt tcacaatcgc
    12661 gttcaggctg ctgacggctt tgacgacttc gggcgcggcg acaatgatgc cggtgcgcac
    12721 gcccggcagg ccgactttgg aaaggctgaa gcagaggatg atgttttcat gccaattcag
    12781 cgttacgtcg ctgtaaatga tgttggggaa cggcattccg taggcgttgt cgataatcag
    12841 cggaatcccg tgttcacgcg ccaaagcgtc caaacgcgcc atttcgccgt cggtcaacac
    12901 attgccggtc gggttggtcg ggcgcgaaca gcaaatcgcg ccgattttgc ccgctttgag
    12961 ttcgggcagg ttttccagtg cgtcaaagtc cacgcggtat ttgaagaagc ctgcttcgcc
    13021 ttcgtgttcg acgttttcga ttttgggctt gacggaaacg aagtgctgcc cttcgacatg
    13081 cacgtcggca tagccgatgt attcgggtgc gagcggcaac aaaatggctt tttctgcgga
    13141 tgtgccgtct gaaagcttga atttgccgcc gaagaggttg aataaataga aaaacgcgtt
    13201 ttgcgaaccg ttggtcagcg cgatattgtc ggcggtcaaa ttccagccgt attcgcggtt
    13261 gaggaaggcg gtcagcgcgt caatcagcgc ggtatcgcct tgggggttgg agtagttacc
    13321 gatgttctcg acagcgtgtt ctgccgccag tttggagaat atgtcggcga acgcccgatc
    13381 gatttccgga atgcgcgccg gattgccgcc gccgagcatg ttgacgggct tgtcgctttt
    13441 gagcgcgtcg ccgaggtcgt ccattagttg gaggatgccg ctgtgttgcg tgaatttttc
    13501 gccgaatgct gaaaactgca tgtcaaactc cgtgtgtgta aggtaggttg gtttgtacgt
    13561 tatgccatct gaaggttcag acggcatttt ttctgtgtgt ttctcgtacc caaagcaggt
    13621 cgcagatgcc gtcgctcggg gcaaagccgg tcagggcgtt gatgagcagg gtgcggattg
    13681 cctgctggtc gtaacggtcg caggccgaac ggatgtggtc gagcagggcg gagagctcgt
    13741 gccacggcag catggtctcg ttggcggtca tgatgcgcgg atggccggtt ttgcggacgt
    13801 tgtcgccgat gagcagctct tcgtagagtt tttcgccggg acgcagtccg gtaatgagga
    13861 tttcgatgtc gccgtcgggt tgttcgggtg ttttgggttt gaggccgctt agggtaatca
    13921 tttgtcgggc aaggtcgatg attttgacgg attcgcccat gtcgaggacg aatacgtcgc
    13981 cgcccgtacc catcgcgccc gcctgtatga cgagttgggc ggcttcgggt atggtcatga
    14041 aataacgtgt gatgttgggg tgggtcaggg taagcgggcc gccttctgca atctgttttt
    14101 caaacagcgg gacaacggag ccggacgaac ctaaaacatt gccgaaacgt accatgctga
    14161 agcgggtttt ttgtccgggt tcggcggcga gcgcctgaag gcagagttcc gccatgcgtt
    14221 tgctggcccc catggtgttg gtggggcgga cggctttgtc ggtggagatg aggacgaagg
    14281 ttcttacgcc cgaagccgtg gcggcaagcg cgcattcgag tgtgccgaaa atgttgttgc
    14341 gtatgccttc gacggtgttg aattcgacca tggggacgtg tttgtaggca gcggcgtgat
    14401 agacggtcgc aacggaaaag gcggtcatga cgtgttcgag cagcgtgcgg ttttgcaccg
    14461 aaccgagaaa gggcaggatt tcggtgtcga ggcgtttttg gatgcaggtt tcgcgcaatt
    14521 ctttttcgat ggcgtacagg gcgaattcgg ataactcgaa cagcagcagc ttttcggggc
    14581 ggcgacggat aatctggcgg cagagttccg aaccgatgga gccgcccgcg ccggttacca
    14641 tgacggtttt gccttcgatg tcggcattca tcaggcggtc gtcgggcgtg acggggtcgc
    14701 gcccaagcag gtcggatacg gagatttttt tgagcgtgcc gatgctgagt ttgccgtcca
    14761 tcaggtcttt gatgccgggg atggtcagca cttcgcacgg ataggcttcc agtttgccga
    14821 tgattttgcg gcgttgcgcc tgggtcgcgc cggggatggc gagcaggatt tttttcacgc
    14881 cgcagcgtcc gatgagggcg gcgatgtcgt cgggacggta aacggcaagg ccgcagatga
    14941 cggtgtgcca cagtttgggg tcgtcgtcca caaaggcggc ggcggaatat tcgcgcattt
    15001 gtttgacggc ctcgagaagc tgtctgcccg cctgccccgc gccgtaaatg atgacgggga
    15061 tcatctgttt tttggggtgt tcggacaaca gtccgcgcaa aaccatacgc gagccggtca
    15121 cggaaacaaa cagcagtaag aaatagacaa tcggcagggc gaggcgcagc ctttcttcaa
    15181 aaatcaatgt gttgaggaag aacaggacgg cggaaacaat gctgccggca aaagcggtgg
    15241 tcaggacgtg gaaactgaca aagcgtgtaa cggcacggta gagtcccatt ctgacgaaca
    15301 ggccgatggt caacggcgcg gtcaatagga aagactgcca gttggcaggg tcgaaccatt
    15361 cgttcgagta gtcggccttt aggctttggg tgaaccaaaa ggcaatgaaa atcatcagaa
    15421 aatcgtgtat gaggaaacag attttcttga tgttgcgcgg tagggcaagc agggtttcca
    15481 gagtcatgtt gtcggcggcg tgtgtttttc agacggcata tgccgtctga agggttatcg
    15541 tgtggcttcg gtcaatacgg cttcgatgtg ttctttgcag aacgcgattt cgccgtcggt
    15601 cagcgttggg tgcaccaaga acatcaggct ggtgtcgccc agttcgacag catttttcaa
    15661 acgctctttc ggccgccacg gcgtattgtc gaaggctttt tccaaataca cttcggagca
    15721 gctgccttga tagcagggga ctttgcgcgc gttcagttcg ttcacgatgc ggtcgcgcgt
    15781 ccagccgtct ttgaggtgtt cgggtttgac gaaggcgtag aacttatatt gcgcgtgtcc
    15841 gatgtagtcg gcgacttcaa ccaagcggat acttttgaat ttgcctaggc tttccgccag
    15901 cttggcggcg tgcgcttgac ggcaggcagt ccattcgggc aggcgtttga gctggatgcg
    15961 cccgattgcc gcctgcattt ccatcatacg ccagtttgtg ccgaaacttt cgtgcagcca
    16021 gcggaaaccg ggcgcgtgtt cgcggtggta cacggcatcg tagcttttgc cgtggtcttt
    16081 gtacgaccac attttttccc acagggtttt gtcgttggtc gtaaccatgc cgccttcgcc
    16141 gcccgtggtc atgattttgt cttggcagaa cgaccatgcg ccgacgtgtc cgatgctgcc
    16201 gacggatttg cctttgtatt tcgcgccgtg cgcttgggcg cagtcttcga tgacccacaa
    16261 atcatgttct tttgccaagt ccatgatgcc gtccatttcg gcgggcatac cggcgaggtg
    16321 gacgacgatg atggctttgg tattcgacgt cagcgcggct ttgatagttt cggcgctgat
    16381 gttttggctg ttcaaatcca catcggcaaa cacgggattt gcgcccgcgt tcacaatgca
    16441 ggaagcggaa gccaagaaag tgcgcgaggt aacaatcaca tcgttgcccg cgcctatgcc
    16501 gattgctttg agcgcgacat cgagtgccag cgtgccgttg gaaagggcga cggcgtaccg
    16561 cgtgccggca aaggcggcaa attctttttc aaatttgcgg cattcgctgc ccgtccagta
    16621 gttgactttg ttggacagca ggactttgga aacggcatcg gcttcttctt gggtaaagca
    16681 gggccacggg gagagggagg tgttcagcat ggtgtttggt ccgtcggttt tcagacggca
    16741 tttccgaccc tatgccgtct gaaggggggc gtgttccgaa gaatcgggcg cgcgccgcag
    16801 gtgttgtcaa aatcggtctg tacgggggtg catagtggat taaatttaaa ccagtacggc
    16861 gttgcctcgc cttgccgtac tatctgtact gtctgcggct tcgtcgcctt gtcctgattt
    16921 aaatttaatc cactatattt taatcgctta tgccgtcccg gtctcggggt ttttgcgcgg
    16981 cagcggcttt gccggattgc ccgcgacggt catgccgtcc gggatgtcgc acacgataac
    17041 cgcccccgcg cccattcaac ttccatcgtt tccaaatggt gtcgaaaacg tagggcggaa
    17101 agccgtttac gccccatgaa taatattttg tcatgaaata gtccttttaa tattgtgagt
    17161 gcatgtttga ttatgtaatg gttaagtatt tgaatcaatt ataggaacta aatcttgaaa
    17221 ggagttaacc caaaagtcgg gatgatattc tgtgttaaaa ctttcaggct ggtggcggtg
    17281 gatgttctgt ccggagggaa gcaatcctat ggttatccag cccaaagtgt tcggcgcgat
    17341 aaagtctttg gaaatgttgt cgccgatata gatgaagcag cctgccttgt cggcatattt
    17401 gtcttgaaga tggcggaagc gtttgctgtc gggcttttcc gaagagcagg cttcagagat
    17461 gaggatgtcg tcaaacaggg aagacagacc caaggcttcc agcttcaggc gttgggtcag
    17521 gcttctgccg tcggtaatca gcgcgcgtgc ggcgaagggc gcggtcagtt cggacaggaa
    17581 atccggcgga gcatacggag tcagcgtcgg gcggtgcagc cggtattgcc acaaaagaac
    17641 ctgtttttcc gattcattaa aaccacagtg gcggcacaat ttgtccagcc aatctttgcc
    17701 gtcggggtcg atgttgcgcc ataggttgtc tgaattccaa tcgggataca gtgcggcaac
    17761 ggtatcgatg accgaacgga taccggagag cttgtattcg tattccgaat acagggtatc
    17821 gtccaagtcg aagacgatga cggtggtttc aggattaatc gttttcatga accaagacct
    17881 tggcatcgta gcgcaacata atgaggttgt tttcccagtc gtctgaaaac gggatttctt
    17941 cgtcgccgag gtattcgcgc atcaaccggc tcggataatt gccgcctgcg gcatacgtca
    18001 gcgggaagcc accgccgaag cgggggttga tttccacgcc gtagaattcg ccggtttctt
    18061 cgttgtagaa gaactgcacc gtgatacagc cgcgcgcgcc ctgcaatacc gacatttttt
    18121 ccagcagcgt ttggtagagg ctgtttttgc aggtaacgcc tttgctgatt tcgccggagc
    18181 gcacttccaa gcgttttcgc gggatggcgc atttcagacg gcattgacgg tcgtaataca
    18241 tatcgacggt aaactcggaa aatttatttt cgatgtcgat aaactgacag aacattaatt
    18301 tggtgtctgc caagacttcc ggtgtcaggt cggcaggtgt gtcaatccgt tttgcaccga
    18361 tggcgcggct gccgtcataa ggcttggcga agcaagggaa aaccaacttc ttgcggtcgt
    18421 aaatttcggg tgagcgtatg ccgtagcggg caaacaaggt ggcggttttc cgtttgtcgc
    18481 ggcattgtgc gatgaagtcc gaatcggaaa cgacgatgtg gacacttgcc gcttcaaagc
    18541 gttcgcgctc gtctgccagc ttttgcagtt cggtgtctat ggttgggatg attaaaccga
    18601 tattttcgcg taccgccaga ttgaaaatgc tgtcgatata tgaatcggta ctaatcggcg
    18661 gcacttcaaa tgaaccgtcc gaaacatggc aggcagaaga catgcggggg ttgaggtcag
    18721 tggcaaaaac gctgatgccg tctgaaaaac gtgcggcttc ggtttggaag tcctgcacca
    18781 attcgacacg gcgacctgcc gacagaatca gtatattatt tttttcatta gtttgatttc
    18841 cagtgaaata aggcatagtg gcttcccctt ccgccgaaat cccttctttg ataaagactt
    18901 ttctgactgt caaaaacagg attttcatat ccagccaaaa actgtaatgg tcgatatacc
    18961 aaacatcgca ggcgaatttt tcgtcccacg aaagcgcgtt gcgcccgttg acctgcgccc
    19021 agccggtaat gccgggtttc atttcgtggc ggcggttttg gaagttgtcg tacagcggca
    19081 gatattgcat cagcagcggg cgcgggccga ccaggctcat gtcgcctttt aagacattcc
    19141 ataattcagg cagttcgtcc aaactagcgg cacgcagttt tttgccgaag ggtgtgagcc
    19201 gctcgctgtc gggcagcgga atgccgtctg aatcaagcgc gtcgcgcatg gaacggaatt
    19261 tgaccatttt aaaaggtttt ccgtcctttc cggggcgttc ttgaatgaag aagacgggcg
    19321 aacccagatt cttgcggatg aggtacgcca aaatcaggaa aacgggtgag aggacaatca
    19381 gccccgatgc ggatgcgatg atgtcgaaca ggcgtttgac ggctttactc atttgccaat
    19441 ctttcaatca ggttgacgat tttccgatag gaaatgtcgc gcctgaagcg gcggacgatt
    19501 tcatcggact gaacggggtc gtttttgcgt ttcaaaatat ctttggcggc ttgaacgaag
    19561 ctgtccacgt cgccggaacg gtagttttcg tgcggcagca ggttcaagag gctaaggact
    19621 tcggcattgg tctggctgtt cagaatcggt ttttgcaaag ccatatagtc ggagagtttg
    19681 ttggtaaccg actgcatggc gtggctatgg atggcgttga cggcaatgtc gcagcctttg
    19741 gcaatcgaca tcatttcaga atagggtaaa taaccgtaaa acttgatggc gcgattttca
    19801 tattgtttga gtttctccaa atcgggtcct ccgcccataa tgtgcaattc gacgttttcg
    19861 ccgtcgtcta aaagtttccg aacgcctttg cacacggttt ccacgtcata gctgtaactg
    19921 agcgtgccca agtagaaaag gcggacggtt ttggagcgga atcgcggggg ggggggcgat
    19981 ggcggcaaaa tccgtaccga tatagacggc ttcgccgggt acgttcggat tggcttcttt
    20041 ggcgcggttg agataagtct gcgatacggc aaccagcgcg tcggcgcagc ggtaggcacg
    20101 gttggcgcgt gaagcaaagg gcagcagctt gtgcggtact tttttcaaaa acggtacgac
    20161 cgaggagaaa gactccggcc atacgtcctg cacatcgaca atcagtttgt agcccaaacg
    20221 cgctttgtgt ttgcccaaca gcaggttggt tgccatcagc ggataggcgg aatagacgat
    20281 gtcttgttcg cccggccggc agtttttcag ccattgctca aaatttttga cgaagcggta
    20341 atggctggca acacgttcta aagacacgtt tttgccgtag ccgctttctt ccaacagcat
    20401 gactttcaga cggccttgcg aggcggcctc ggcatcttcg ggccgtctga aggatttgtc
    20461 gtagtggcgg aaacggctgg taatcagcaa cacgtcgtgc gattgcgaca atcgttctgc
    20521 cagataccaa aagcggttga aatacggttc ggacggcagc gagcaataag gggcgactat
    20581 ggtgatgttc atcggcggct ttcagacggc attacatggt atacgccgcc atggtggtac
    20641 ggtagatttc gtcgtcggaa cagagtgcgg cgacgtgtgc gtgcaacctt ttgcccattg
    20701 cttcgcgcaa cagcggctgt ctgacgagcg tgtctacggc ggcgataaag gcttcttcgt
    20761 cgccgaaggg gatgcagtaa cccgtttccc cgttgacgac catttcggaa atgcccgcca
    20821 tatcgtaggt aacgacgggc gtgtcgtaaa gtccggcttc taagatgttg ttgcccacgc
    20881 ctgcgccgtg gtcgccgatg cagtggggcg tgttcaccaa aatatccacg tctttgaagt
    20941 aggcggtcaa atcgcgcacg ccgccgagga aggtaacttt gccgtctatg cccagacgtt
    21001 ccgcctgcgc tttcaaattg tccatttctt cgcctatgcc cgccacgttc aggcgtacgg
    21061 gcatatcgcg gtcgaccatt tttttgaaaa tgtccagcat cagatgcacg gcgcggacgg
    21121 tgtccaaacg ggaaagcgtg ccgagtacgg cgcattcttt ttcggttttc cggaaatgaa
    21181 attccggggg ggggggggag tgcgttgtag gcgtaggcaa tccggccggc ggggaagccg
    21241 tggcggatga gtttttcgcg ctcgtgtttg cagttgccga tgatgtacac gcccaacttg
    21301 tcgaagagtt tggcgatttt ggggtaggtt gccgcgtcca aaccgtgcga gtggtagatg
    21361 attttggttt tcggcgaaac gattttggcg gcaagggcgc aggcgggaac gacgcgcgcc
    21421 atttgacagt cgatgatgtc gggttgttcg cggcgcagca tccgggcgta ggtaaaggtg
    21481 cttttgaggt agccggcaag cccgccccgg taaaaatcaa ccggctgcca acggatgccc
    21541 gatgcttgcg cttctccgac aaaaggcccg tccgaagaag caaggatgac gtcgtgcccg
    21601 cgccgtttga gtagccgccc gaggcggacg gtggcggttt ccgtgccgcc caagcctgcc
    21661 atagaagtgg tcaggatgat tttcataatg gaaacccttg ttttttcaaa taatgaaaca
    21721 gtttgtgcaa atctttccgg tggcgcagga tgcagcctgc tagatatgcc gcccatacgc
    21781 cggcaaacag gggatagttt gccggcgtgc cgaagcaggt gtaggccgcc gaggaggcca
    21841 ggcagaacaa tgtgtgcaga taaagcggca ggcgtttgag cggctgccac aggcggcagg
    21901 agctttcggt cttgaaggca aaaaacagcc agaatgaggc ggcacaggca accgccgcgc
    21961 cgcgcgcgcc gccggatggt acggaaagcc ccagcagcag caggtttgcc gccagcgcgc
    22021 ccaaggtggc gagcgcgatc gggcgtgttt ttcggacgac gttcaaaccg atgccgctga
    22081 tttctaccag cgtgcaaaac agcggcggca gcatacacga tacgacgata aaccggacgg
    22141 cggcgtagtt ttccggcagc aggagggagg cgaggggcga gaaaatgccg gtcaggcaga
    22201 cggcggcggc aagcagggcg gaggcggatt ctgccgttgc cgagaggcgg gcgggcgggg
    22261 tgtttgcttc gattgcgcgg aaaatatacg gtgtccagac cgttgaaaag atgctttgga
    22321 acaataatgc cgccccgccg aacgaaatac ccatcgaata aacgccgagc tgttctaggc
    22381 cggcatattt tttcaggaac aaacggtcgg cggatgccag cccccaatag gcgatgctgc
    22441 ttagtgcgat cggtatgccg tagcgcaggc cgcgatgcag gacggcggat gaaaacggtg
    22501 cgcgccggac ggccttcaga cggcatcggt tttgaaacag caaaaaggcg gcggcggcaa
    22561 ggtttgccag cgcgtaaacg gcggtcagga cggcggtgtt cgccggaaag tgcagcagcc
    22621 cgaccgtcag cggcagcagc agcaggatgg cgagcttgga cacgagttgc gcggacgaaa
    22681 aggcaagggc gcgcccttcc atacgcaaaa ccagtaagag aaagcggatg ggcaggaagc
    22741 tcagttcaaa cagcaccagc ccgatgccgg cggcggcatc gtcgagcgaa aacaggattt
    22801 cagacggcag ggatgggcgg gaaagcagca gggcggctat cgcggcggca gacagcagcg
    22861 gcggcaggaa cagggttttg aacaaagtgt ctttgtcggc ggcggcatag tattcgcgga
    22921 cgtatgcctg atccagcccg aggcacaata ccgacaccgt cagtcccgcc gccgtctgca
    22981 tcagcacgat gcgcccgatg tcgtcggcgg ggaaatacca cgacagcagc ggcaggatga
    23041 tgacggctaa aaccgcgctg ccgatcgagc ctgccgcgta gccgaggatt tcttttgtgt
    23101 ccatttttga tgtccgggcg gcggcgggat gctgcctgtg ccgtctgaag cctttcttga
    23161 tcggaatttg acggctttca gaccgtcgcg gctgccggcg gggtgcggca gtccgggttg
    23221 cgttttttcg ggtgggcggc ggtctgaatg gtgccgtttt ttatcggcgt tattatatag
    23281 tgaaacggcg caaacccttt aaaaggcgtt gccgtttttt cggaacacgg ttttgatgtc
    23341 gtgtccgagg atttcgcttg aaacgggtgt ccacaagggc ggtgcggaaa gggcggcgcg
    23401 gttttcgagc agggaaaaca ggtctttgcc gctgccgagg attttgggcg aacggtacag
    23461 gacgatttcg tctgccagat tttctgccag aaatgcggat gtgagttcgg agcctgcttc
    23521 gaccatgatt tcgccgaaac cttcgtcagc aaggaggcgc atcaggtggt gcaggtcgat
    23581 tttgctgtct gccgtttcag acggcatcag gatgcggacg tgtgcgtgtt cccgataggg
    23641 gtgcagtctg tcttcgttgc gttcgagtgt ggcgatgtag gttggagatt gtccgtcggt
    23701 cagaaggtgg ctgtccaagg gcaggcgcag gcggctgtct aacacgatgc gtgcgggttg
    23761 gcgcaaagtt ggaaaagcgc ggacgttgag ccggggattg tccgccaaca ccgtgccgat
    23821 gccggtcagt accgcgcagc tttcggcacg caaaacctgt acgtcggcac gcgcgtcttc
    23881 gccggtaatc caaaagctgc tgccgtctga aagggcggtt ttgccgtcca gcgaaacggc
    23941 gcatttgagg cggacaaagg ggcggcggcg ttcgatgcgc gacaggaagc ctcggttgag
    24001 ttcccttgcc tgatgttcga gtaaaccgca ttccgtcttg atgcctgctg cttcgagcat
    24061 ggcaaaccct ttgcctgcaa ccggcgggtt ggggtcgcgc atggcggcaa cgacgcgtgt
    24121 tacgccggaa cggagcagag cttcggcgca aggcggtgtg cgtccgtaat ggctgcacgg
    24181 ttcgagggta acaaaggcgg tcgcgccttg tgccatttcg cccgcctgac gcagggcgtg
    24241 gacttcggca tggggttcgc ccgctttgac gtggaagccc tgcccgacaa tttggctgcc
    24301 gtgtgcgata acgcagccga cgcgcggatt gggcgaagtg gaaaaacgcc ccaaagcggc
    24361 aagtcggagg gcgttttcca tcatggatat atctgtgtcc gaaaacatag ggataccgta
    24421 tcagtatggg ttgggggaat caggctttgc cgcctgtttt gacggcttgc gccagccacg
    24481 aggcaaagtc tgccggattg tcgaagcgtt tgtgcagggc ggcgaaacgg acggcggctt
    24541 ccgtatcttg tcggagcagt tcttttagca ccatgtcggc aagtgcggca gaggggatgt
    24601 cgcgctgacc cgaagtgtag agcctgtgtt ccgtcaggcg gacggtttcg tcgatctgtt
    24661 cgggtgtcag ggcggatttt cgggcggcgg cggtcaggtc gttgcggagg cgttgtgcat
    24721 tgaagggcga acgttttttg tccggaccga tgacggaggg cattttgagt tcggcggttt
    24781 cgagcgtgcc gaagcgtttg ccgcagttgg ggcagtggcg gcggcggcgc acggcgttgc
    24841 gttcttccat cagacgcgaa tcggcaacgc gggtgtcggg gtgggcgcaa aacgggcatt
    24901 tcatggcgtt tcttcctgat gccgtctgaa cgtcaaaccg atacgccggc ggcgcgggcg
    24961 atttccagac cttcttcggc actcatatag acggggtttt cgggacggtc gtgtcggacg
    25021 atgttgcctt cgcggaacat gaccagttcg tccacggcaa gttgggacca tgattcgtcg
    25081 cgggtcagtg gcagggtggc gataacggcg acgcggtcgg agggcgtggt tacttcggca
    25141 aaatcgacca tcacgtcgtc gtcgagcagg cgcgccttgc cgaacggggc ttggcggacg
    25201 atgtagtgca gcagcgtgct ggcgtgggca aacagggcaa tgccgtctga aagcataaag
    25261 ttaaacagcc cgaacttgcg gatttcgtgc gtcagccccg caatcgcgtc aaacagcgtg
    25321 tcgtcgtcgg gacgggcggc aaagcgggtg cgcaggcggt tgaggatgtg gcagaacgcg
    25381 cgttcggaat cggttgtgcc gacggggtgg aaaaattcgc cctgttcggg gaaaaaatca
    25441 atcaaatgtc cgttgtgggc aaacagccag tagccgcccc acatttcgcg cataaaggga
    25501 tgggtgttcg ccagcgaggt ttgtccttgc gatgctttgc ggatatgtgc gatgacgttt
    25561 tccgatttga tttggtaggc acgcacgagg tcggcgacgg gggaatttgc gctcggcttg
    25621 tcgtcgtgga acaggcgcac gcctttgcct tcgaaaaagc cgataccgaa accgtcggcg
    25681 tgatggtcgg taatgccgcc cctgcggcgg aagccttcaa aggaaaacat aatatcggtc
    25741 ggcgtattgc agttcatgcc cagcagttga cacatggttt gtcccaatga ttcagatggt
    25801 cgcaagtatt cggattatac cccgaactga aaatgccgtc tgaaatacgg cttgttcccc
    25861 gttattccct cgaaaacaga aaacagaaaa caaagacgga aacttaagat tccgtcattc
    25921 ccgcgcaggc gggaatccga cttgtccggt tttggttgtt tttcgttccg taacttttga
    25981 gccgtcattc ccgcgcaggc g
//
LOCUS       DAWFKN010000022        25046 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFKN010000022 DAWFKN010000000
VERSION     DAWFKN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.022"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(1032..2933)
                     /gene="thiC"
                     /locus_tag="WGR46_000909"
     CDS             complement(1032..2933)
                     /gene="thiC"
                     /locus_tag="WGR46_000909"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0855876.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(3024..3123)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3273..3349
                     /locus_tag="WGR46_000910"
     tRNA            3273..3349
                     /locus_tag="WGR46_000910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3307..3309,aa:Met,seq:cat)
     gene            3531..4406
                     /locus_tag="WGR46_000911"
     CDS             3531..4406
                     /locus_tag="WGR46_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0855877.1"
                     /translation="MPTVHFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            4415..5353
                     /locus_tag="WGR46_000912"
     CDS             4415..5353
                     /locus_tag="WGR46_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0855878.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(6073..7848)
                     /gene="ptsP"
                     /locus_tag="WGR46_000913"
     CDS             complement(6073..7848)
                     /gene="ptsP"
                     /locus_tag="WGR46_000913"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0855879.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNLNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
     gene            complement(7848..8117)
                     /locus_tag="WGR46_000914"
     CDS             complement(7848..8117)
                     /locus_tag="WGR46_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0855880.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALSDLINDYFGEGE"
     gene            complement(8186..8623)
                     /locus_tag="WGR46_000915"
     CDS             complement(8186..8623)
                     /locus_tag="WGR46_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0855881.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(8705..9268)
                     /locus_tag="WGR46_000916"
     CDS             complement(8705..9268)
                     /locus_tag="WGR46_000916"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0855882.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(9337..10161)
                     /locus_tag="WGR46_000917"
     CDS             complement(9337..10161)
                     /locus_tag="WGR46_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0855883.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRVRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVHARTDR"
     gene            10253..10885
                     /locus_tag="WGR46_000918"
     CDS             10253..10885
                     /locus_tag="WGR46_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0855884.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGSHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNHWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(10953..12743)
                     /gene="rmuC"
                     /locus_tag="WGR46_000919"
     CDS             complement(10953..12743)
                     /gene="rmuC"
                     /locus_tag="WGR46_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0855885.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEVERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(13117..13902)
                     /locus_tag="WGR46_000920"
     CDS             complement(13117..13902)
                     /locus_tag="WGR46_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0855886.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEFSKDIH"
     gene            complement(13917..15266)
                     /locus_tag="WGR46_000921"
     CDS             complement(13917..15266)
                     /locus_tag="WGR46_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0855887.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDTNKPVPERVTMSTTKQKIMFFVYVGITVVGAYLFATNI"
     gene            complement(15285..15866)
                     /gene="petA"
                     /locus_tag="WGR46_000922"
     CDS             complement(15285..15866)
                     /gene="petA"
                     /locus_tag="WGR46_000922"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0855888.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(15989..16738)
                     /locus_tag="WGR46_000923"
     CDS             complement(15989..16738)
                     /locus_tag="WGR46_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0855889.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            16869..17798
                     /locus_tag="WGR46_000924"
     CDS             16869..17798
                     /locus_tag="WGR46_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0855890.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(18053..18445)
                     /gene="rpsI"
                     /locus_tag="WGR46_000925"
     CDS             complement(18053..18445)
                     /gene="rpsI"
                     /locus_tag="WGR46_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0855891.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(18455..18886)
                     /gene="rplM"
                     /locus_tag="WGR46_000926"
     CDS             complement(18455..18886)
                     /gene="rplM"
                     /locus_tag="WGR46_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0855892.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(19064..19243)
                     /gene="ssrS"
                     /locus_tag="WGR46_000927"
     ncRNA           complement(19064..19243)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGR46_000927"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(19249..19554)
                     /locus_tag="WGR46_000928"
     CDS             complement(19249..19554)
                     /locus_tag="WGR46_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0855893.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(19565..19894)
                     /locus_tag="WGR46_000929"
     CDS             complement(19565..19894)
                     /locus_tag="WGR46_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855894.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(19949..20938)
                     /locus_tag="WGR46_000930"
     CDS             complement(19949..20938)
                     /locus_tag="WGR46_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0855895.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(21061..23763)
                     /gene="ppc"
                     /locus_tag="WGR46_000931"
     CDS             complement(21061..23763)
                     /gene="ppc"
                     /locus_tag="WGR46_000931"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0855896.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKTDGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHVDVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            23806..24699
                     /locus_tag="WGR46_000932"
     CDS             23806..24699
                     /locus_tag="WGR46_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0855897.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGTAR"
BASE COUNT         5471 a         6722 c         6639 g         6214 t
ORIGIN      
        1 ttttaagttt cgggtaactt ccacttcgtc attcccgcgc aggcgggaat ccagtgcgtt
       61 gagtttcagc tatttagaat aaattttgaa actttaaccc cgtcattccc acgaaagtgg
      121 gaattcagaa cgtaaaatct caagaaaccg ttttacccga taagtttctg tgccgacaga
      181 cctagattcc cgcctgcgcg ggaatgacgg gatttgaggt tgcggcattt atcgggagca
      241 acagaatccg ctctgccgtc attcccacga aagtgggaat ctaggacgca aaatctcaag
      301 aaatcgtttt gcccgataag tttccgcacc gacagaccta gattcccgcc tgcgcgggaa
      361 tgacgaatcc atccatacgg aaacctgcac cacgtcattc ctacgaacct acattccgtc
      421 attcctacga aagtgggaat tcagaacgta aaatctcaag aaaccgtttt acccgataag
      481 tttctgtgcc gacagaccta gattcccgcc tgcgcgggaa tgacgggatt tgagattgcg
      541 gcatttatcg ggagcaacag aatccgctct gccgtcattc ccacgaaagt gggaatccag
      601 gacgcaaaat ctcaagaaac cgttttgccc gataagtttc cgcaccgaca gacctagatt
      661 cccgcctgcg tgggaatgac ggcggaaaga ttctattttt cccgataatc acccacaatc
      721 tcaaattcct tcattctctc aaaaacaaaa tcagaaccct aaatcccatc atccccatct
      781 atgtgaatat aaaaatttta aaaattatag tggattaaca aaaaccagta cggcgttgcc
      841 tcgccttagc tcaaagagaa cgattctcta aggtgctcaa gcaccaagtg aatcggttcc
      901 gtactatctg tactgtctgt ggcttcgtcg ccttgtcctg atttttgtta atccactata
      961 ttttcacaag cgaaagaatg ccgtctgaag ccttttttcc gggtttcaga cggcattttt
     1021 tgcttgacgt ttcaactgta aatcttcgcg ccttttttga cgaactcgac cgctttttcc
     1081 tccatgccct gccgttgggc tttttgcttg tcggcgtagt cgcgcacttc ctgcgtgatt
     1141 ttcatcgagc agaatttggg gccgcacatt gagcagaagt gggcgatttt cgcgccttcg
     1201 gcaggcaggg tttcgtcgtg gaagctttcg gcacgttcag ggtcgaggct taagcgaaat
     1261 tggtcgcgcc aacggaactc gaaacgcgcc ttgctcaggg cgttgtcacg taattgtgcg
     1321 cccggccagc ctttggcgag atcggcggcg tgggcggcga gtttgtaggt gatgatgccg
     1381 gtgcgcacgt cttctttgtc gggcagcccc aaatgttctt tgggggtaac gtaacaaagc
     1441 atcgccgtgc cgtaccagcc gatattggcc gcgcctatgc ccgaggtgat gtggtcgtag
     1501 ccgggtgcga tgtcggtaac gagcgggccg agtgtgtaaa aaggtgcttc aaagcagtgt
     1561 tgcagctctt cggtcatgtt ttctttgacg cgttgcagcg gcacatggcc ggggccttcg
     1621 atcatgactt gtacgtcatg tttccacgcc ttatcggtca attcgcccaa ggtgtgcagc
     1681 tcggcgaatt gggattcgtc gttggcatcg gcaatgcagc cggggcgcag gccgtcgccg
     1741 aggctgaacg acacatcata cgctttcatg atttcgcaga tttcgtcgaa atgcgtgtag
     1801 aggaagtttt cccgatgatg tgccaaacac catttcgcca taatcgaacc gccgcgcgag
     1861 acgatgccgg tgaggcggtt ggcggtcatc ggtacataac gcagcaacac gcccgcgtgt
     1921 atggtgaaat agtccacgcc ttgttccgcc tgctcgatta aggtgtcgcg gaacaaatcc
     1981 caagtcaaat cttcggcaat gccgccggtt ttttccaaag cctgataaat aggcacggtg
     2041 ccgatgggga cgggcgcgtt gcggataatc cattcgcgcg tttcatggat gtgcgcgcct
     2101 gtggataagt ccataattgt gtccgcgccc caacgcagcg accacaccat tttttcgact
     2161 tcttcggtca ggctggaagt gacggcggaa ttgcccaagt tgccgttgat tttgacgcgg
     2221 aagttgcggc cgataatcat cggttcgagt tcgggatggt tgatgttggc ggggataatc
     2281 gcgcgtccgg cggcgatttc ttggcgcacg aattcgggcg tgatttggtc gggacgggtt
     2341 gggatgttcg cgccgaaact ttgccctgcg tgttgtttca agagtttggc gtattccgat
     2401 ttttgggaca attcgtctaa ttttaaacgt tcgcgtatgg cgacaaactc catttcgggc
     2461 gtgataatgc cttggcgcgc gtagtgaagc tgggttacgt tgctaccgct tttcgcgcgg
     2521 cgcgggcggg tgatttggtt gaagcgcaga tgggcggttt tcggatcgtg tgcgcgttcg
     2581 atgccgtatt cgctggagag cttgggcagg atttcggtat cgccgcgttc gttcagccat
     2641 gcggtgcgga tgtgcggcag accttgtttc aggtcgatgt gtgccgctgg atcgccgtac
     2701 acgccgctgg tgtcatagac gggaatcggc gggttggctt ccgcgccttg cgccgtatag
     2761 gtgtcgtcct gacggatttc acgcaaaggc acgcggatgt cgtcgcggct gccttgcaga
     2821 tacacgcgtt ccgagttcgg atatttgaag cggataccga tgtcttcgct caagtcggca
     2881 agttcgcgcg cttcgttgcc ggaagttttg gcggtttttt ttggcgtagt cataaaaaat
     2941 gctcctgttt tctcgtttag aataaagaaa caggagcgtt ttgcgttttc agacggcatt
     3001 tgaaaaccaa tgctgtctga aagacagaat ccgtgaaaac tccccacgca ggtattatcc
     3061 cgatcgggtg taaagggtat ttctcagccg cctgaacatc atgcagcacc cctgtttcaa
     3121 tgttaaccaa aattaaatca cgaacatgaa cttttgtaaa gaaaatgata tttcaaatca
     3181 ggcataaacc gccggacggc aaaattttat gatttttcgc ggaaataatg tttgacaaca
     3241 taaaaaatct gccgtatagt ttcgttttct gacgcgggat ggagcagcat ggtagctcgt
     3301 cgggctcata acccgaaggt cgtaggttcg aatcctgctc ccgcaaccaa atatcaaacc
     3361 cctcggttca atgccgaggg gttttgtttt gcctgtttcc tgccgcctcc gttttttgcc
     3421 ggattttcct tcctgccgca atatcggaac agcagcccgc cgtctgtttg cggttgcaaa
     3481 ttcaggcggt ttgattacaa tcttccgcat tatgttcaag aaagccgact atgccgaccg
     3541 tccattttac cgaatccgtc agcaaacaag accttgatgc cctgttcgag tgggcaaaag
     3601 caagttacgg tgcaaaaagt tgctggaaaa cgctgtatct gaaccgtctg cctttgggca
     3661 atctgtcgcc ggaatgggcg gaacgcgtca aaaaagactg ggaggcaggc tgctcggagt
     3721 cttcagacgg cattttcctg aatgcggacg gctggtcaga tatgggcggc cgcttgcagc
     3781 acctcgcccg cacatggaac aaggcggggc tgcttcacgg atggcgcaac gagtgtttcg
     3841 acctgaccga cggcggcggc aatatcttgt tctcactcga acgcgccgct ttccgtccgt
     3901 tcggactgct cagccgcgcc gtccatctca acggtctgac tgaatcggat ggcagatggc
     3961 atttttggat aggcaggcgc agtccgcaca aagcagtcga tcccgacaaa ctcgacaata
     4021 ttgccgccgg cggtgtttcc agcggtgaat tgccgtctga agccgtgtgt cgcgaaagca
     4081 gcgaagaagc gggtttggat caaacgctgt ttccgctcat ccgcccggta tcgcagctgc
     4141 acagcctgcg ccccgtcagc cggggtgtgc acaatgaaat cctgtatgtg ttcgatgccg
     4201 tcctgcccga aaccttcctg cctgaaaatc aggatggcga ggttgcgtgt tttgagaaaa
     4261 tggacatcgg cagcctggtt gccgccatgt tgtcggaaaa catgatgcac gacgcgcaac
     4321 tggttacgct ggacgcgttt tgccgttacg gtctgattga tgtcgcccat ccgctgtccg
     4381 agtggctgga cggcatacgt ttataggatg cgccatgctt gaactgaacg gactctgcaa
     4441 acgcttcggc ggcaaaacgg ttgccgacga tatctgcctg actgtcgggc gcggcaaaat
     4501 actcgccgta ctggggcggt cgggctgcgg aaaatccacc ctgctgaata tgattgcggg
     4561 catcgtccgg ccggacggcg gcgaaattca gctgaacggg gaaaacatta cccgtatgcc
     4621 gcccgaaaaa cgccgtattt cgctgatgtt tcaagattac gcgctgtttc cccatatgag
     4681 tgcgctggaa aatgcggcat tcggtttgaa aatgcaaaaa atgccgaaag ccgaagccga
     4741 acgccttgcc atggcggcac ttgccgaagt cggactggaa aacgaggtgc accgcaagcc
     4801 tgaaaaactt tccggaggcg agaaacaacg gttggcactg gcgcgcgctt tggttgtccg
     4861 cccttccctg ctgctgttgg acgaatcgtt ttccagtttg gacacgcatt tgcgcggcac
     4921 gctgcgccgc atgactgccg aacgcatccg caagggcggc atccctgccg ttttggtaac
     4981 gcattcgccc gaagaggcct gcacggcggc ggacgaaatc gccgtcatgc acgaggggaa
     5041 aatccttcaa tgcggtacgc ccgaaacctt ggttcaaacg cctgccggcg tgcaggtcgc
     5101 ccgactgatg ggtttgccca ataccgacga tgaccgccat attccgcaac atgcggtgcg
     5161 tttcgaccaa gacggcatgg agtgccgcgt attatcccgt acctgtttgc ccgaatcgtt
     5221 cagcctgtcc gtcctccatc cggaacacgg catcctgtgg ctgaacctcg atatgccgca
     5281 cgccggtgaa atatcgggaa acgatacggt acgcatccat atcgaagaca gggaaatcgt
     5341 ccgcttccgc tgatgcttct taaaaacaaa atgccgtctg aaaacctttc agacggcatt
     5401 ttttaccaaa gcagccatat tttttatcag ggctgcaaaa tttatccgaa acaacagaaa
     5461 ccaccctgcc gtcattcccg caaaagcggg aatctagaac ccaacacggc aaaaatttat
     5521 ccgaaacaac aaaaatcttt tcatcgtcat tcccgcaaaa gcgggaatct agaacgtaaa
     5581 atctaaagaa accgtttttc ccgataagtt tccgtgccga cagacctaga ttcccgcctg
     5641 cgcgggaatg acgggatttt aggtttctga tttcggtttt ctgttttaag ggaatgacga
     5701 gacttgagat ggcggcattt atcgggagca acagaaacca ccctgccgtc attcccgcaa
     5761 aagcgggaat ctagaaccca acacggcaaa aatttatccg aagcgacaac aatcttttca
     5821 tcgtcattcc cgcgcaggcg ggaatctaga acgtaaaatc tcaagaaacc gttttacccg
     5881 ataagtttcc gcaccgacag acctagattc ccgcctgcgc gggaatgacg gattttaggt
     5941 ttctgatttt ggttttctgt ttttgaggga atgaccgatt ttgggtttct gtttcggttt
     6001 tctattttgc aagaatggca aaatttcaga ttgcgggcat tgttaagtat ttctattttt
     6061 tacctgccgt atttatttcc gccccttgaa gtcggcttct tcctcgacag acacgctgtt
     6121 gatctgtttg atcagctttt ccgacttctc ttcgtcttcg cagcggatga ctttcacaat
     6181 atcgctttcg agctgtccga cattgctgtg cagaatgatg tttttgacgg gcaggatgtt
     6241 gttgaggttc atggaaaaac ggcgcagccc catacccaat aaaacgcggg taaacgcggt
     6301 atcgcccgcc atctcgccgc atacggatac gtctttgtcc atgcggttgg cggtacggat
     6361 gacgtgttgc agcattttca gcacggcggg atggccgggc tggtagaggt ggctgacgct
     6421 gtcgtcgccg cgatcgacgg acaagatgta ttgaatcagg tcgttggtac cgacggagat
     6481 gaaatcgacc agtttcaaaa tactgccgac ggtcagcgcg gcagacggaa tttcaatcat
     6541 acagccgatg ccgactttac cgaaggcatc gccgcgttcg gcaagctggc gttgcgcggt
     6601 gtcgaggtgg atgaggcact ggcgcacttc ggatacggag gtaatcatcg gccacatcat
     6661 ccgcacgggg ccgtgggcgg cggcgcggag gatggcgcgc atctgggtgc ggaacatgac
     6721 cggttcggca aggcacaggc ggatgccggt catgcccagc gcggggttga ggctgccgtt
     6781 gggcgtgctg tttttcccga accagcgcgg gtttttgtcc acacccaaat cgactgtccg
     6841 tatcgttacg cttttgcctt tcattttttt gacaatcgcg ctgtacactt cgtactgctc
     6901 gtcttcagac ggcatcgtat cgcggttcag gtaaagaaac tcgctgcgga acagcccgat
     6961 gccgtctgcg ccgaggttgt gcagcggttt cacgtcttcg gcggattcta tattgcccac
     7021 aagctcgatg cagaccccgt cagcggtggc ggcggcggtt tttttgagct tgttcaaatc
     7081 gcgtttgtgg ctgcggtatt cgcgggcacg gcggcggtat tcgttcaaca ccgactcatc
     7141 cggcgcgata atcaacacgc cgttgatacc gtccacaatg accgtttcgc cttcggtaat
     7201 cagtttgcgc gcgttgtgca gcccgacgac ggacgggatg tccaagctcc tgcccaaaat
     7261 cgccgtatgc ccggtggggc cgccggcatc ggtaacgaag gcggcaatgc gctgctcttt
     7321 aaacaaaacc gtgtcggcgg gcgaaaggtc gtttgcaatc agaacggttt cgtcaaacag
     7381 gttgtcggca acttccaact cgttgccctg cccgatcagg ttgttgtgga tgcggcggac
     7441 gacttgcagc atatcctgct tgcgttcgcg caaataggca tcgtccatat tgtcgaattg
     7501 ggcggcgagt ttgtcgctct gctgcttcaa tgcccactcg gcgttgattt tttgttccct
     7561 taaaatatcg acgggttcgc gcgacaaggt aacatcggtc aagagcatca ggtgcagcga
     7621 gatgaacgcg cccaactcgg tcggggcgtt ttcgggaatc gcgctgcgga gctgttccaa
     7681 ctctttgcgc gtggctttga cggcggcatc gaaacgttcg gcttcggcat cggtgtccgc
     7741 ctccgcaaca tcatactgcg gcacttcctc cgtaccgcgc gcaatcaggt gggcgcaacc
     7801 gacggcaata cctttacccg ccgccacgcc gtgcagcacg atactcatta ttcgccctcg
     7861 ccgaagtagt cgttgattaa gtcggacagg gcgcgcatcg cttccgcctc gtccgcgccg
     7921 tccgtctcca gctcgatgac cgtacccttg gcggcggcga gcatcatcag ccccatgatg
     7981 cttttgccgt tgacgcggct gccgtttttc gttacccaga cttcgctttg aaactgagac
     8041 gcggtttggg tgaatttgct ggaggcacgg gcgtgaagtc cgagtttgtt gatgatttcg
     8101 atggattgtt tgagcatttc gattcccgtg ttatgtatat cggcagcaga cgccgtttaa
     8161 aatgttttcc tgccctgccg cttcttcaga cggcatcgcc gctgcgccgg cacaccaaat
     8221 cttcgggcgc ggacgtgatg gcgaaaatgc cttttacagc cgcctctctg acacattcgg
     8281 taaaggcggc aaggtcttcc gccgccggcg aatattggac ggccttaacc atcatcggcg
     8341 cgttcagtcc ggtcaaaatc gccgatttgt tttcgcgcac gaggcggcgg gctgcattgc
     8401 agggggtcgc gccgaaaata tcggtcataa tcagcacgcc gtcgttgtcg ggaaattcct
     8461 gaagcgcggc aatggcgttg ttgatgatgt cgtcttggtc ttccgtcggt tgcacgccga
     8521 gtatgcggac gttttcaggc agcccgcccg ggaaaaaatg atgtgccagc ttgcggtagg
     8581 cttcgcctat ggtttcgtgt gtgatgatta aaagccctat catattgatg cgtcctgttc
     8641 ttcattatcc tgccggcgta tgggcgcgat gccgtctgaa aggccttcag acggcatcgc
     8701 gtccttattt tccgtccaat gcgtaaatct cgcccaaatt acgccagcag cccgccgcat
     8761 ccatgccgta accgaaaaca taacggttcg gcacatccag tccgacataa tcggctcgga
     8821 taggcttggg tttgtcaatc agcttgttgg cgaacaccgc cgcacggcag cttgccgcac
     8881 ccatttccaa aagtttggct tgaatggcgg acatcgtatg cccttcgtcc aaaatatcgt
     8941 ccagcacgac gacgtgcctg ccccggattt gttccgcatc gggcatacgc ttccagttga
     9001 acgcgccgcc ctccagcttg tcgccgtaac gggaaacgtg aacataatca aaatctaagg
     9061 gaaaacgcaa cagcggcagc aactgccccg taaacaccac cgcgccgccc atcacgggca
     9121 gcagcagcgg atatttgccg cccaaatcgc gcgtaatctc gtccgccact ttttgcagtg
     9181 cggcacggca ttggccttgg tcgaacaaaa gatcggcgtt ttcaagcatc gcctgtgttt
     9241 caaggcgttt ggtttctaaa tcggtcatat gtcggaatcg gtcggtaaaa ggaaaattat
     9301 aaaccaaagt atcggctgcc gtctgaaccg tcctgctcaa cggtcggtac gcgcgtgcac
     9361 aaacgtggcg aatttcggcg tgcctttctg cgtaaagcca cggtaacggt aggtaatcag
     9421 cgtaccgatt ttgggcgggt tgtcgcggtc tttatctttg aagccgctgc cgatgcggaa
     9481 ttcgccgtgt cggtttttgc agccgaccgc gcccaaccgt ccggcgtttc gccctttgcc
     9541 ctcatagtgc cgcgttaccg tgcattcgtc gtcgtattgg cttttcagct tcaataattg
     9601 gctgctcctg ccgccgctat aacgggattc gggctggcgc agcatcacgc cttcgccgcc
     9661 ctgcgcttcg atttgtttta aaaagtccat cgcgtgctgc cggacgcgca ctttgatttg
     9721 cgggatgatg gtaatcggcg cgttcggatg cgttttcagc cactgcgttg cgactgccaa
     9781 acgttggtag aggttgccct gcgccttggg tacatcgaaa acgtgcaggc ggatgccgcg
     9841 ccaatctgaa gaagcagaac gcacggcggc ggaaatctgc tcgaactgac cgcgtccgct
     9901 atacaattcg ccgtccaaag gataaggcgg aaactgagcg gtaaaacctt tggggggcgt
     9961 aaacgcatag ccctgacggc ttatcaggtg ctttccgtcc caatacgcgc gcacgccgtc
    10021 gagtttctcg ctcatcgccc agccggcaat atcctgccct ttgtattcct gcgccagcat
    10081 caaatccgcc gcgcctgccg atgcagggat gaaaaccgcc ataaaaatcg gtatgatgcc
    10141 gccgattgtc ttcttaatca cccgattccc ccaatgtcca aacaggcggc aaaccgccat
    10201 aaaacaaacg gcaaacccga tgccgtctga aaaaccgttt aggaacacgc cgatgaccct
    10261 acgttacgaa atcttccccg ttaccccctt ccgccaaaac tgcaccctga tttgggacga
    10321 cgaaagtggc gaagccgtcc tgaccgatgt cggcggcgat gtgccgttcc tgctgcaagc
    10381 gttggcaaac cgcaaactta cgctcacggc aatctggctg acgcacggcc atctcgatca
    10441 cgcgggcggc gtggtcgaaa tgttgaaaac gcataaagtc cctgtcctcg gttcgcaccg
    10501 cgaagatgaa ttcctgctcc aatcgctgcc gcaaaccacc gcgcaatacg gattccccgt
    10561 ctcgcccgcc tttgcgccga accattggct cgaagaaggc gaaacgctca aagtcggacg
    10621 ctacgccttc caagtgctgc atattccggg gcacacgccg ggacacgtgg ttttctattg
    10681 tgccgaagcg gagctgctga ttgcgggcga cgtgttgttt tacgaaacca taggcagaac
    10741 cgattttccg cgcggcaacc acgccgactt aatcaataat atccgcaaca aattattcac
    10801 ccttcccgaa accgtgcaag ttgtcgccgg acacgggcgt atgacttcca tcggacacga
    10861 aaagcggcac aatccgtttt tctaaccgcc ttccctacgg tcttcagacg gcatcatctg
    10921 cactgatgcc gtctgaaacg catccgcctt tcctattcgg cttcgtcagc cgcttcctgt
    10981 tccgaaggtt tgcccaaagc aaatgccgtc tgttcgttgg cacgctcgac caaatcccgt
    11041 tgaagctgtt tgcctgcctt cacgcccaac agacgcagtt tctcggcgcg gccgaccaga
    11101 ttcccgcgcc cctcggcaag ttgcttgaat gccgtctgaa agctgttttg cgcctgatcg
    11161 atgcctttgc cgacgctttc gagcgtctgt acaaagccga caaacttgtc atacagcttg
    11221 ccgccttcgt ccgcaatcgc cagcgcgttt tggttttgct gctcgttgcg ccaaatattc
    11281 gccaccgtcc gcaaagtcgc cagcagcgta ctggggccga ccagcataat ccgtttgtcg
    11341 aaacactctt ggaacaagcc agcgtcattc tgcaacgcca acaggtaggc cggttcgaca
    11401 gggataaaca taaagacgaa atccaatgtg ttcacacctt ccaaatcggt gtaatccttc
    11461 agcgacaagc ctttcatgtg tgcacggatg ctggcaacgt gtgccgccag ttcgcgtgcc
    11521 gccgtatccg catccgccgc ctgcgtgtag cgcacataag ctgtcagcga gaccttggaa
    11581 tcaatcacaa tctgcttgtt gtcgggcagg ttgaccaaaa cgtcgggctg gaggcggcgc
    11641 gtgccgccgt cttcctcttt tcggacggat gccgcctgaa ccacatattc ccgccctttc
    11701 tgaaggccgg aattttccaa aaccgtttcc agaatcatct cgccccaatt accctgaacc
    11761 ttattctgcg taccagtcag cgcgttggtc agggcctttg cctcgctgtg cagctgcgca
    11821 ttcaatcctt gaagccgttt caattcgttt tccaacgtca gccgctcgcg cgattcttta
    11881 tcataggttt gcttgaccaa ctcgccgaaa ccgtggatgc gttcgtttag cgggttcaaa
    11941 acctgatgga gctgctcgcg gttctgctcg gtaaaacggc ggcttttttc ttccaaaatc
    12001 gtgttggcaa gattttgaaa ctgatcgctc aaactcttgc gcgcctcgcc cagcaaggac
    12061 agcttctctt cagaagcaag gcgttcctgt tcgatttgcg ttgccaaacg ttcgttttca
    12121 accgccaaac cctgtgcctt ttcctgcaac tcggtatgcg actgcctcaa ccgctccacc
    12181 tctgcctctt tttcctgcaa atgggcaatc tgtttttcgg ctgcggcaaa acggttgccg
    12241 acatcggaaa ggtcgttttg cacgtcgcgg acagtttggc ggctttcttc caaatcggtt
    12301 tcgattcttt ggcggatttg gcgttccaag gcatattggt cggcaaacgc cttccgttcc
    12361 tgtccgagct gcgttgccaa acgttcgttt tcaaccgcca aaccctgtgc cttttcctgc
    12421 aactcgatat acgactgctt cagccgcgcc gactccgcct ctttttcctg caaatgggca
    12481 atctgctttt cggctgcgga aaaacggttg cccaaagcat aattttcgtc ctgcaaatgc
    12541 cggtatttcc cgtccaacac cgccaattcc gacacggttt tgccgtgtgc ctgttcgaca
    12601 aaatcacatc ttgccgcctt ttccgccagt tgcgcgttca aaccggcaaa ctcgccctga
    12661 aacctgccct tcatcagcaa ccatgtaaac aacacgcccg acaccaacgc cgccaaaggc
    12721 agcaaaacag tcatcagttc catcaatcat cctaatattc aaacattttc acaccggaca
    12781 aaaccgccgc ttattccgat tctacctgtt tgttcgacat agctccaaaa aatatagcgg
    12841 attggcttta aacctgttcg acatcgcctt accatgctgc ttgcggtttc agaccttttc
    12901 ctaattcaat atcaatctgc cacaaaccct gattaagttc ccgatgtctg acatttttag
    12961 aatgatgccg tctgaaatgt tgcagctatg ttcagacggc atacggattc aggcttttca
    13021 aacggcaggc aaaatgaaaa aagggcaaac cctaaaggat ttgccctttt gttccaaacg
    13081 cttagtgtac gtctttccaa tattcttttt taatgattaa tgaatatctt tagaaaattc
    13141 tttatttagg aagtaagcca aaggcagcat aaccgcaaat aggaaaatca tcacgacata
    13201 gcctatacgt ttgcgttgca gttgtgcagg ttcgcccatg tacacaaggt aattgaccaa
    13261 atcgcgtaca tatgcgtcgt actctttttg gatcactttg ccgttaggca ggcggcggct
    13321 gtgcaaaccg gtagattccc aatacagctt aggcttcatc tcgccgtgtt cgtcttttac
    13381 cataaccggc tgacctttgg catccaactc aacggcttga acaccttgtt gctcccacaa
    13441 cgggtggggc ataccgactt tatcgaatac agtattgttc cagccgctcg gacgggtcgg
    13501 atctttatag aagccgcgca tataggcgta aaggtagtct gcacctttgg aacgcgcaat
    13561 caacgtcaaa tcgggcggag cagcaccaaa ccattttgcc gcatctttcg ggttcatcgc
    13621 cgaatgcatg acatcgccga cattatcggt ggtaaacatc aggtttttct tgatttcttc
    13681 gtcagtcaaa ccgatgtctt tcagacggtt gaagcgcata ccgcttgcag agtggcaaga
    13741 caaacagtag tttgtaaaga tttgcgcacc gtgctgcagg ctgacttggt cacgcaggtc
    13801 gatatcgact ttttcgtagt gtccgccgcc gctggcgacg gctgcactca taggcactgc
    13861 cagcaataag gcagcaaacc agtttttcag agtttgtttc attttcgctg ccctcatcag
    13921 atattggttg caaacaagta agcaccaaca acggtaatac cgacgtaaac aaagaacata
    13981 attttttgtt tagtagtgct catggttacg cgttcaggaa ctggtttgtt ggtatccagt
    14041 ttggtataga acggcatacc caggaagaat gcaaagtaga cgaaagacag gatacgtgca
    14101 accaaagtac gagtatcagt tgctaccatt gcacccaaaa tacccaaacc gatgaaggca
    14161 atgatgaaca gaaccaatgc ggttttgaag attgggccgc gatagcggac agatttaacc
    14221 tcgcctttat ccaaccaagg caacagggca atcagtacaa ctgccgcacc cataccgatt
    14281 acaccccata cctgtgttcc caagaacgag ggaatcgcac gcaaaatggc gtagaacgga
    14341 gtgaagtacc ataccggtgc aatgtgcgga ggtgttttca gcgcattcgc agcatcgaag
    14401 ttcggcgctt cgaggaagta gccgccgcct tctggtgcaa agaacatcac ggcacagaag
    14461 acaatcaaga atatcgttac tgccaatata tcatgcacaa cataatacgg aaaaaaaggt
    14521 atgccatcta gagggacacc gttttcatct ttcagctttt tgatttctac accgtcaggg
    14581 ttgttggaac ccacttcatg caaggcaatg atatgagcca caaccaagcc gagcaatacc
    14641 aaaggtacag cgataacgtg cagggcgaag aatcggttca aagtaacatc ggaaacgttg
    14701 aagtcaccgc ggatccaagt ggacaaatca ggaccgataa cagggatggc ggagaacagg
    14761 ttaataatta cctgcgcacc ccagaaggac atttgacccc aaggcaacag gtaacccata
    14821 aaggcttctg ccatcaatgc caagaaaatc agggaaccga aaatccacac caattcgcgc
    14881 ggttttttgt acgaaccgta aatcagacca cggaacatgt gcagataaac gacgatgaag
    14941 aagaaagatg cgccggtaga gtgcatatag cggataatcc agccgccgga cacgtcgcgc
    15001 atgatgtact ctactgcggt aaaggcagca ggcagatggt aggcgttaag gttgccgtcc
    15061 ggtttgtagt tcatggtcag gaaaataccg ctgacgattt gaatcaccag caccagcata
    15121 gacaatgagc cgaagaaata ccagaagttg aagtttttag gcgcatagta ttcagacaga
    15181 tgctctttcc acattttact taatggaaaa cgggcatcta cccagcctaa caatgctttt
    15241 gctttgctat tggtttggtt tgccataatt atcgttcctt attcttagtc ttcgcccacc
    15301 aagatagttg tgtcgctcaa gtatttatat ggcgggacaa ccaggttggt cggggcagga
    15361 acacctttat atacgcggcc ggccaagtcg aatttcgaac cgtggcaggg gcagaagaag
    15421 ccgcctttcc agtctgcacc caaatcggcg ggggcaatgt cgggacggaa ggtgggcgag
    15481 cagcccaaat gggtgcagat accgatggcg acaaggatgt tcggcttaat cgaacgggtc
    15541 tcgtttttag catactccgg ctgctgttcc gcatcggaat tgggatcggt aagttcgccg
    15601 ttcaggcctt tcaggtcttt aagctgctga tctgtacggt tgagcaccca aatcggtttg
    15661 ccttgccact cggcggtcag caactgaccc gcttcgattt tactgacatc cacctcgacg
    15721 gcagcaccgg cggccttggc tttttccgaa gggaaaaaac tggccacaaa cggcgttgcc
    15781 acacccaatg ctgccactcc gcccgcgccg caggtcgcga gtgtcaggaa acggcggcgg
    15841 ccgttgttga tttcttgatt atccattatt cagtcgtcct aatattttgg gaataccgag
    15901 ccattaaacg ttgcaatttt acccagtttg cagtgatact caaagcatta tttaaaataa
    15961 ggtaaagttt tatgatattt ctcaagactc aagccggatt gttttcgtca aaatggcaca
    16021 cttccaaccc gaaaacctct gccgccgatt ctgccagcgc gcgtacgccg taacgttccg
    16081 tcgcgtgatg ccctgccgaa atgaaagccg tacccgtttc attggcaagg tggtattgag
    16141 cttcagagat ttcccccgtc aaatacagat cgacaccttc gtctattgcc gtctgaaaaa
    16201 acccctgcgc cccgccgcta caccatgcaa cccgtcggat ttcgcgttcg ggtttgccga
    16261 tgacgacagg cttacgttgc aaaactgttt caatatgcgc cgccaatgcg ccgagtgtct
    16321 tggcttgttt caggctgccc gagttgagca ggttttgttc gccgaaccgt ttttctgtcg
    16381 caaaacccaa tctgtcggcg agttgggcat tgttgcccag tgtgggatgt gcatccaggg
    16441 gcagatggta gcctgccata ttgatgtcgt gccgtaacag tgcggcaatc cgttcttttt
    16501 tccaaccagt aacggtcggc aactcgtttt tccagaacat accgtgatgt accaaaagca
    16561 aatctgcctt ctgctccaca gcaaaatcaa tcgctgccct gcttgccgtt accgacgtaa
    16621 cgattttccc gatatattcc ctcccttcaa cctgcaaacc gttaggggcg taatctttaa
    16681 acaacgctgt ctgcaatgtt tcattacacc aagtcagaaa atccctgcgc aataccatct
    16741 tttttcctaa tcgctttaaa caagcgggca ttctaatcgc aaaatgtccg gaattcacat
    16801 ttttccgatt tgcacccgca tatgaattat tttaatatgc gccggttcaa tatgccgtct
    16861 gaagccccat ggattccatt atcgaattgc gccacctcaa aaccctgctg gcacttgaag
    16921 aaaccggcag cgtctccctt gccgccaaac gggttttcct tacccaatcc gccctttccc
    16981 accagatccg tatgctcgaa aaccactacg gcacgccgct gttcgaacgc aaatccacgc
    17041 ccttgcgctt taccccggtg ggcgaaaggc tgctgcgcct cgcccacgaa ctgatacctc
    17101 aagttgctgt tgcagaacga gatttggcgc gaatcacaga aggggaggcg ggagagctgc
    17161 ggattgccgt cgaatgccat acctgtttcg actggctgat gcccgccatg ggcgaattcc
    17221 gcccgatgtg gccccaagtc gaattggata tcgtatcggg attccaagcg gatcccgtcg
    17281 gactgctgct gcaacaccgt gccgaccttg ccattgtttc cgaagcggaa aaacaaaacg
    17341 gtatttcttt ccaaccgctg tttgcctacg aaatggtcgg catttgcgca ccagaccatc
    17401 cgcttgccgc caaaaacgtt tggacggcgg aagactttat cggggaaacc ctgattactt
    17461 atcccgttcc cgacgagatg ctggatttgc ccaaaaaaat cctgattccg aaaaacatca
    17521 acccgccgcg ccgacacagc gaactgacca tcgccattat ccaactggtt gccagcagac
    17581 gtggcattgc cgcccttccc tattggacag tcatgcccta ccttgaaaaa ggctatgtcg
    17641 ttcaccggca gattaccgac gacggactgc aaagcaaact gtatgctgcc atccgcactg
    17701 aagatacgga caagagctat ctgaacaact tttgccaaat catacgtgaa cgcggttttg
    17761 cagatttgcc cggactgagc gaactggaat cggtctgacc ccttattcaa ccatacccgg
    17821 cagtttttct attttttcat gtatagtgga ttaacaaaaa ccagtacagc gttgcctcgc
    17881 cttgccatac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    17941 actatactgt ttttgatttt tgcccaatct gtaatcttta gattgccaat ggggaaccgt
    18001 ctactacaaa taaaaaaccc tgcgataagc agggtttttt gaatttccaa cattaacgtt
    18061 tggagaattg ttttgcacgg cgtgctttgc gcagacccgg ttttttacgt tcgacttcgc
    18121 gggcatcgcg ggtaacaaaa ccagcttgag acaaggcggg tttcaacgcg gcatcgaagt
    18181 cgatcagggc acgggtaatg ccgtggcgga ttgcgccgga ctggccggtt tcgccgccgc
    18241 caacaacatt gactttgatg tcgaaagatt cggcgttttc agtcagaacc aagggttggc
    18301 gaacaaccat tcggctggtt tcgcgtgcga agaattcgtc aacgggacgg ccgtttacga
    18361 tgatttgacc tgtaccttta atcaggaata cacgagccac tgaacttttg cggcggcctg
    18421 tgccgtagta gtatttaccg ttcatgtcgc gtccttattt cagttccaaa actttgggtt
    18481 gttgcgcagc atgggcgtgt tccgcacccg catacacttt cagtttttta atcatggcgt
    18541 aacccaaagg acctttgggc agcatacctt ttacagcttg ttccaaagcg cggcccggga
    18601 attgctcttg catttcgcgg aaagtgcgtt cgtagatacc gcctgggaaa ccggaatggc
    18661 ggaagtattt tttatcttcg aatttggcac cggttacacg cagtttgtcc gcattgataa
    18721 caatgatgta atcgccggta tcgacgtggg gggtgtattc aggtttgtgt ttgccacgca
    18781 gacggctggc gacttcggcc gcaacgcgac ccaagacttt gtcttgggca tcgatgacga
    18841 accattcgcg cttcacctcg tggggtttcg ctgaaaaggt tttcatagtg gaaatccaga
    18901 tagatataga aagttgtaaa ttttaaagac aggattcgat tttgtcaatc gcattaccgc
    18961 gttacggaag gattttccgg atttcggcag actgcatact gctttttcgg gcggggcggc
    19021 ggccaatgtg aaaaaccgca tcgttgcgat gcggttttga atgggaatcc ccgcgagagc
    19081 cgtttcggcc gaatccgctt gaaccttgct gacaaggcgg ctgcctcggg tagtttcggg
    19141 tgcgtccgca aaaggacgct cgcgcccact actgctcccg gcaaccttaa gcgaacttat
    19201 tggttcaaag gaatatatgc cttcgcggac accgcaggga aaaaggggtt attcctgcgc
    19261 caagcgggat agtgcttttt ggcaggcgtt gtccatatcg gctattttac gcgcaaaatc
    19321 gccgattgcc aaatcgccgc cgttcaggga ggttttcaac aggtcgtgga cgacgttgag
    19381 cgcggccata atgacgattt tttcgctgtc cgcgacgcgt ccgccttcgc ggatggcttc
    19441 ggctttgccg ttgagcattc cgactgcctg caacagtgtg tctttttctt ctgccggcgt
    19501 gttgacggtc agccgggcgt gcatgacttc gatgtggact tgttcgatgt tcatccttta
    19561 atccttattg ctgcgtttcc tgccgttggg ggaggcgcgc tgccagtgcg ctgatttttt
    19621 ccctgctctg ttcgagcagg ctgcggtatc gtgtattttc ttctgtcagg ctgtcaattt
    19681 tgttttgcag gtcttctttg agtttgccga cttggacgag cagggcttcg ctaagttcgt
    19741 cgacggcggt ttcgtgttcg agtttttgcc gctcgtgcgc ccgtttgagt tcggcgacgg
    19801 tttctttgag gcggcggttt tcgctgacga gggtttcgaa tttttgtacc agcgtataaa
    19861 cgctgctttc gagtttttcg atattttgtt tcataacctt acctgtccgt atgccgtctg
    19921 aaggcttcag acggcatctg tctgttgttt attcaaaacg cgcgctacgt tccatcagcc
    19981 tttcgacgac ctgctgaggg gtcatttcct tacggatgag ttgcagcagg gtttgggtaa
    20041 tcggcatatc gatttggtac ttacaggcag tattgaagac ttcttctatc gtgctgaccc
    20101 cttcggaaac gtgtccgatt tcgaccagca cctgatgcag ttccttgcct tctgccaaac
    20161 ccaagccgac gcggcggttg cgtgagagtg cgccagtgca ggtcaagatg aggtcgccga
    20221 tgcctgccag ccccatcatg gttttgggct gtgcgcccat tgcggaggca aggcgggtaa
    20281 tttcagctaa tccgcgcgta accagtgcgg cacgggcgtt aagcccgtac tctaggccat
    20341 cggacaatcc ggtggcaatc gccataacat tttttaccgc gccgccaacc gccacgccga
    20401 taacgtcggt actgccgtaa agcctcatga cagtcgtgtt gagctgcggt acgagttctt
    20461 caatccactc ttggttttcg gaggcgagga cgacggcaca gggcagttgt ttggcgagtt
    20521 cctgtgcaaa actcgggccg gagagtacgc cgattttctt attgtcgggc aatacttctt
    20581 tcaatacttg aaaggtcagc agccctgtat cctgctcgaa tcctttgcag gcggcgagga
    20641 cggggaggtg tcccgcgccg tactgtttga gcagctctgc gctgcttctc aatccggcaa
    20701 cggaggttac gataaggaca agtccgctgt ctttgagcgc gtctgccaaa tccgcacaca
    20761 cttccaaggt ttcgggaaag gaaaagccgg gcagtccgcg tttgttttca cgcgcttcct
    20821 gcatttggcg gacttggtct gcgttgcgcg tccacaggga tacgcggttg ccgtgttggg
    20881 aaaagtgcag ggcgagcgcc gtaccccacg aacctgcgcc gataacggta attttcattg
    20941 gtcgtctttc aacatatcac tgccgttcac tttaaaacaa tcggtgtttc tctgcaagtg
    21001 cggtcaggga aatgccgtct gaaaggtgtt cagacggcat tttgccccga tgcggcacaa
    21061 tcagcctgta ttgcgcaaac cttgcgccac gccgttgatg gtcaggtgaa ccatcagcag
    21121 cgcgtgcgga ttgtcgggtt ctttacgcag gcgtttgagc atggcgacct gcaggccgtt
    21181 gagcgcgttc aggtagggaa tcctcaaggc aagcgaacgg gcgaggctgc ggttgtcgcg
    21241 taaaagttct tcggtttgca gcaggtcgag cagggctttg cggctgcgtt ggtattcttc
    21301 cttaatcatg ccgaagatga cggcggcttt ttccggcgat tcgctcaagc cggcatagtt
    21361 ttccgcgagg gtgatgtcgg ttttcgccat cacttgctcc atattggaaa gcatggcttg
    21421 gaagaacggg ttgctttggg cgtgttcgcg cagggcggcg agcgtttcgg gtttgtcttc
    21481 gcacaaggtt tccaccgcgc tgccgaaacc gtaccaagca ggcagcatga ggcggttttg
    21541 catccaggaa aatacccacg gaatcgcgcg caagtcttga atccgcgcta aggtcttgcg
    21601 gctggcagga cggctgccga ggttgagggt ggcgatttcc tgaatcgggc tggtttgcag
    21661 gaagtagtcg atgaagtcgg ggtgggtaat cagttcgcgg tagtatttga acgatacgtc
    21721 cgacaatgcc tgcatcagtt tggcatcagg gtctttttta tccggcagga tgctggcttc
    21781 caaagtcgcg gcaaccaagg tttccaagtt gcgttgggca ttgccggggt cggcgtattt
    21841 ggcggtaatg acttcgcctt gttcggtaat gcggatttgt cccgccacgc tgcccgccgg
    21901 ttgggcgaga atggcttggt aagacgggcc gccgccgcgt ccgacgctgc cgccgcgacc
    21961 gtggaacagg cgcatgcgga catcgtattt tttgaagagt tcgaccaagc ccaattccgc
    22021 ctgatacaga caccatgagc tggtaacata gccgccgtct ttgttggagt cggagtagcc
    22081 gagcatgatt tcttggatgt ttccgcggct ttcgagcagt gcgtcgtacc aatcgaggcg
    22141 gaacatggtt tccatgaccg gacaggcgtt ttcgagtgct tcgatggttt caaacagcgg
    22201 cacgatattg atgcggctgt gcggtttgcc gttttccacc gccaacaggc cgctttcttt
    22261 cagcagcaat gccaaggcga gcaggtcgct gggttgttcg cagttggaaa taatgctttg
    22321 tgtaacggca tcttcgccga attcgtcttt gattttgcgc gcttcgttga aaatcgccag
    22381 ttcgcggcgg gtatggtcgc tgtatgtgat aaacgggctg tacagcggac gttgatggct
    22441 caattcgcgc aacagggcgg tttgtttttg ctcttcgttc aggcggttgt agtcttccaa
    22501 gcctgcgtgt tggaaaagct cggcaaccac atcgacgtgt ttgcctgcgt gttggcgcag
    22561 gtcgagcggc atcatgtgga agccgaacac ggacacggag cggatgaggt ctgccaaacg
    22621 gccttcagca agcaggcggc tgccgttgtc gataagggaa cgttgcaatt ttttcaaatc
    22681 atcgagaaat tcttgtgccg aagcatacgg ctcgagaaag ccgaatttgc agcccatgcc
    22741 caaaccgagc gcgcgcgctt tgcccatagc gcgcgccatg atgtaagcaa tggcgcggcg
    22801 gtagggttct tcggtgcgag cgatttcttc gtcaggcgag agggctgcca gtgccattac
    22861 gtcgtcgttg actttgacgc ggcggatgga gagcggcagt tcgcggtaaa gtttgtcgag
    22921 ttcgccacgg taaaaacgga acacggcatc ggcgtggcgg cggaaggcaa aacgcaaggt
    22981 ttcggcagaa acgaacggat tgccgtcgcg gtcgccgcca atccagccac cgattttgag
    23041 gatattcgga acgcagacat cgggatagac catctgaaag tcgtgttcca tattgcggta
    23101 gagcttgggc agggcttcga aaaagctcat cgggaagatg gacacgccgt tgttgatttc
    23161 gtcgttgacg ctgagtttgt ggcggcgcgt ttcgctggtc tgccacaagc ccagcaggat
    23221 agtgtcgatt tcgcggcgca gccgtgccag cgcgtcggca ttggtgcagc gttcgcgttg
    23281 cggcaacagt gcgcggatgc ggcggttgaa gcttaagacg gtttggcgtt gcacttcggt
    23341 cgggtgcgcg gtcaaaacgg cggtaacgga cgtattgtcc aactgccgct gcaccgattt
    23401 gccgtcggtt ttccccgctt tgagcctgcg gacggtttcc gtcaggctgc cttccgcgcc
    23461 gccgcgtccg gcttcttcgt ggatttggcg gcggcgttcg tggtgcacgt cttcggcgat
    23521 gttcaaaatc tgggcgaaca ggccgcaggc caaggttaaa tcgtgggttt gttgttcgtc
    23581 caattgcggc aatacttttt caatcaatgc cgcgctgtcg tcggaagtgg acaagagttt
    23641 gaccgtttcg acaaccaacg gcgaggcttc ttcgtgcagg aggttgaaca gggattgttt
    23701 cagaaattcc gcgtccgccg ccaaagccgc gtccttcgga ttgttcagaa tatgcagttg
    23761 catgattttt ctctcattgc cgtaaatact gtaaatgtac ctcaaatgcc gcatccgtgc
    23821 caaaccgttc acactttaac cgcccgtgtc ccgaaatgcc gtctgaagtt gaacgccgcc
    23881 cgacggcagc gttacaatcg cccgcaactg ttttttccga acatcatcat gaccacgacc
    23941 gaacacgaca acgacgacga actcctgctg cggtacagcc gccacatcct cttggacgaa
    24001 atcggcatcg aagggcagca aaagctttcc gacgcgcata ttttggtcgt cggatgcggc
    24061 ggtttgggtg ccgccgcact gccctacctt gccgcttcgg gtgtcggcac gctgaccata
    24121 gccgatttcg acacggtcga actgcacaac ctgcaacgcc aagtcgcatt tgacgagggc
    24181 gatgtcggca aactcaaaac cgaagccttg gcaggccgcc tgaaacgcat caaccatacc
    24241 gtcaacgtcc gcgccgtcaa cgaaaaactc gacggctgcc gcctgaccgg tttggttcaa
    24301 gccgccgaca tcgttttaga ctgttgcgac aactacgcca cgcggcaagc cgtcaaccgt
    24361 gcctgcgtgc aaacgaaaac accgctggtt tcaggggcgg cggtacgctt tgaagggcaa
    24421 cttgccgtgt accgtcccga cttgcccgac tcgccgtgtt acgcctgcct gtttgacggc
    24481 ggatcggctt cagacggcat ctgttctctc ttcggcgtgt tctcgccgct ggtcggcatc
    24541 atcggcagta cccaagcggc ggaggctctg aaaatcctgc tggatgcggg cgaaccgtcg
    24601 cacggcaggc tggcggttta ccgtgccttg gaagggggct ggcaatattt cgacctgccg
    24661 cgcaaccctg aatgcccggt ttgcggcacg gcgcgataaa ccctgccgcc gtttcagacg
    24721 gcatccaaac ggatgcggcg gaacggtttt aaaaatttaa aaatttacat ttctttgcaa
    24781 aaaaaaaaaa aaaaaaaaaa aaaaaaaata aacttacctt ataattgcaa ttgttttagc
    24841 aatgtctgtt tcgcagactc attgagtaaa acgttttccc cgtaatgtgt ttggccgtct
    24901 gtcccctttg ggttcggacg gctttttttt ggctgtgttt gaatacccgg ttggttttat
    24961 ctgtttgcag cgggggaagc cgcttatttc cgttcgggcg gaaaacggtt ccatcggata
    25021 aaaggcattt tgtccgactg attaaa
//
LOCUS       DAWFKN010000023        24741 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFKN010000023 DAWFKN010000000
VERSION     DAWFKN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24741
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.023"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..200)
                     /locus_tag="WGR46_000933"
     CDS             complement(<1..200)
                     /locus_tag="WGR46_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855898.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVA"
     gene            complement(218..775)
                     /locus_tag="WGR46_000934"
     CDS             complement(218..775)
                     /locus_tag="WGR46_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0855899.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(851..2485)
                     /locus_tag="WGR46_000935"
     CDS             complement(851..2485)
                     /locus_tag="WGR46_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0855900.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2559..2888)
                     /locus_tag="WGR46_000936"
     CDS             complement(2559..2888)
                     /locus_tag="WGR46_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855901.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2888..3994)
                     /gene="serC"
                     /locus_tag="WGR46_000937"
     CDS             complement(2888..3994)
                     /gene="serC"
                     /locus_tag="WGR46_000937"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0855902.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4359..4790
                     /gene="rimP"
                     /locus_tag="WGR46_000938"
     CDS             4359..4790
                     /gene="rimP"
                     /locus_tag="WGR46_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0855903.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            4818..6320
                     /gene="nusA"
                     /locus_tag="WGR46_000939"
     CDS             4818..6320
                     /gene="nusA"
                     /locus_tag="WGR46_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0855904.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6332..9220
                     /gene="infB"
                     /locus_tag="WGR46_000940"
     CDS             6332..9220
                     /gene="infB"
                     /locus_tag="WGR46_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0855905.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9431..10825)
                     /locus_tag="WGR46_000941"
     CDS             complement(9431..10825)
                     /locus_tag="WGR46_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0855906.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10899..12239)
                     /locus_tag="WGR46_000942"
     CDS             complement(10899..12239)
                     /locus_tag="WGR46_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0855907.1"
                     /translation="MLNPSQKLVELVRILEEGGYIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWHEGT
                     WFTGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLAEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12383..13009
                     /locus_tag="WGR46_000943"
     CDS             12383..13009
                     /locus_tag="WGR46_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0855908.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSI
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13073..14491)
                     /locus_tag="WGR46_000944"
     CDS             complement(13073..14491)
                     /locus_tag="WGR46_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0855909.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSIFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRIIYDDYL
                     MQKKARQDLSYQYEFYEFHDKG"
     gene            14818..15546
                     /locus_tag="WGR46_000945"
     CDS             14818..15546
                     /locus_tag="WGR46_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0855910.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVANDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15605..16093)
                     /locus_tag="WGR46_000946"
     CDS             complement(15605..16093)
                     /locus_tag="WGR46_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0855911.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGIEPCVLCISQRLC
                     VLATALCAVIVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPPCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGIALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16782..17246)
                     /locus_tag="WGR46_000947"
     CDS             complement(16782..17246)
                     /locus_tag="WGR46_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0855912.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17471..18529
                     /gene="alr"
                     /locus_tag="WGR46_000948"
     CDS             17471..18529
                     /gene="alr"
                     /locus_tag="WGR46_000948"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0855913.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIKLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(18709..19380)
                     /locus_tag="WGR46_000949"
                     /pseudo
     CDS             complement(18709..19380)
                     /locus_tag="WGR46_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(19523..20878)
                     /locus_tag="WGR46_000950"
     CDS             complement(19523..20878)
                     /locus_tag="WGR46_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0855914.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(20893..21165)
                     /locus_tag="WGR46_000951"
     CDS             complement(20893..21165)
                     /locus_tag="WGR46_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0855915.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(21291..22028)
                     /locus_tag="WGR46_000952"
     CDS             complement(21291..22028)
                     /locus_tag="WGR46_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0855916.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(22309..23208)
                     /gene="prmB"
                     /locus_tag="WGR46_000953"
     CDS             complement(22309..23208)
                     /gene="prmB"
                     /locus_tag="WGR46_000953"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0855917.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            23353..23946
                     /locus_tag="WGR46_000954"
     CDS             23353..23946
                     /locus_tag="WGR46_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855918.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGTLTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            24058..24495
                     /locus_tag="WGR46_000955"
                     /pseudo
     CDS             24058..24495
                     /locus_tag="WGR46_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         6567 a         6670 c         6047 g         5457 t
ORIGIN      
        1 tgcgctacca cttctgattc cccgataccg atgccgtctg aaccttcaga cggcatgaga
       61 cctttgcctg cgtacttggt gcgctggtcg cctccgaaca tggcgcgaca cccgacattt
      121 ccgccgaacg catcgagcgt ttcatgaatc cggtttaaaa cgcatggaaa aatgccgtct
      181 gaaagccttt cagacggcat ttcgcttgag attccgttta ccaatggctg acaaacgctt
      241 ccaaatcggt attcttgggc ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc
      301 cgatgatttt atccttatcc gcacatccga aagcctcacg caacaggggg ctgttgaccc
      361 acatccccgt aatccagaca ttgtcgaaac cttgcgccgt tgccgccagt tgcagcgcat
      421 acgccgcaca acccgccgtc agcatctgct cccattccgg tttcggctta ggcacatcgc
      481 ggttcggcgc aaacgttaca ccgataacca tcggcgccat attgcccact ttttccgcct
      541 ttttcatcgc atcgtcgccg aaattcaatt cggcaaccgt ttgctttaac acatcgcgaa
      601 aacgttgcaa tcctacctcg ccttgaatca cggtaaaacg gaaggggcgc atattgccgt
      661 gatcgggaac ttgggttgcc gcctgaaata tttgttccaa ctccgccgca tcgggggcgg
      721 ggtgcttcag ctttttggaa gatcggcggt tcgtcaataa ttttaaagca tccatatcgt
      781 tatactccgg tcatcggtcg ggtgttcgga cacatatgcc gtccgaaggc ttcagacggc
      841 atatccggca tcagcgcgga cggcggcagg ctgccaatat atccatttcc ttccgatagg
      901 tttggctgtt ggaaatgtcc atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat
      961 attcgttttc cgccgctttt tgtttgaggc attggaaatc tatgcccccg tgttggcgga
     1021 aggctttgga aaaccacata accatcggga tatgcgtctg cccggaaggc gcgatggcgt
     1081 aaggcgcggc gtgcaggtac atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat
     1141 aatgcaccac gctttccaaa tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa
     1201 actggtccac atacaaaacc gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt
     1261 tgttgatttc gttggtgtcg caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt
     1321 cgtaatacgt cggcccgtgg ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt
     1381 ttttgttgag gacttcgtcg aacttggtca gcaggatatt gtcgaggcac tcgccgttgc
     1441 ggcagtattc gggcaggttg agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc
     1501 ccttgcagcc ggaatcgttt tccaaccagg taacttccac gccggcgcgc tgcacgatgt
     1561 ccagcaggtt gtcttggtgt tcggctttga tttcgtcata atccgtgcgg tcgaaggttg
     1621 agaacataca cggcagggag tgcgcggtcg atgtaccgca gcttctgacc tgcgggaaat
     1681 tgacaatttc atcgccgcgc gcggcaagca gcggcgtagt ttggcggctg taaccgttca
     1741 aaccccagtt ggcggcacgc gtggtctcgc ccacgaccag caccacgaaa cggcgcaggc
     1801 tgccggccgg gcggttttgc acgaccgcca tatccaattg cgtataagga atattggaac
     1861 gcttccaatc tttgtatttc gacacgcccg cgccgatgaa attagatggc acaatcagat
     1921 gggttactga tttattgttg cggaaaaacg aggcgtaatc ctgatattgc aacattgcga
     1981 tgcccaacgc gcacaaaaag gaaacggcgg caagcacaag gcgcgtcaaa agctccttat
     2041 accaaacgcg gtatttaacc ttgacggcga tatacgccag cgcgggcaat acgcccaaac
     2101 atacaatcca cagcacatag cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt
     2161 gcaagacatt attcaacatc gacttgttga aatagatatt gaaaaatatt tcttggtaag
     2221 acaccgccgc actgataacc aatatcaacg gaatcaatac cttatgcacg aaaggcaggg
     2281 caatgacgtg aaaaacgaaa ttacttaaaa aaaacagcac caccggcatc gtatagagga
     2341 agatatccgc cccggtgccg ttaaaaggat gaagctcgac aactttggca aaaaaggcgt
     2401 aattcaatac cagcgaggaa tacagggaaa ggaaggcaat cagcgcggaa gagccgagct
     2461 tcggcctcag gttcggtttt atcatttgga atgtgtcgga taagggttgg aaaaggcatc
     2521 cggcatttgg aatccggatt attgaaaaag attcttaatt ataaggcaac ggagcaaagc
     2581 agggcaagaa aacggcggct gtgcgggggg ttccgcccgc cattcaaacg tccggcagac
     2641 ataaaaacat cgtaagcaag attcgaaccg gtctgcaacc gcccctgcca gaaaaacggg
     2701 caaagcattt catattggaa aaacccggcc gcgccgccga cgggacagtc cggcacaaac
     2761 agcatcacgc ccggattgaa aaggacggat agccgccggc agcacaatct taccacctcc
     2821 aaaacgccgc cggagaacgc cgaaacagcc gatgccgtct gaagccgctt cagacaacat
     2881 cgggacatca accgtaacgc cgttggaaat cgcgcataaa atctgccaaa gcccgcacgc
     2941 cttcaagcgg catcgcatta taaatgctgg cacgcatacc gccgacggtt ttatagccct
     3001 taagcaggca caagccctgc aattcggctt ccagcacaaa acggcggtca agctcctcat
     3061 cccccgtttg gaacacgaca ttcattttag aacgcgcatt cggacggata cggttgatat
     3121 agaaaccgcc gctgccatct atcgtctcat acaaggtttg cgccttcagc cgattgaccg
     3181 cttcaatttt tttcacaccg ccctgcgcct gcagccagcg gaacaccagc cccgacatat
     3241 aaatcgcgta agttgacggc gtgttgtaca taccgtcgcg gttcaaatgc gaacggtagt
     3301 tgaacacatc gggaatatcg ttcggacaac gctcgagcaa atcctcacgc acaatcacca
     3361 ccgtaacccc tgccggcccg atgttttttt gcgcgcctgc gtaaatcagt ccgtagtcgg
     3421 caacatcaaa ctcacgcgac agaatctcgc tggacatatc gcacaccaac ggcggcatgc
     3481 cgtctgaaag gcgaggcact tcacggtatt gcagcccgtt gaccgtttca ttgacggcaa
     3541 aatggacaaa cgccgaatcg ggtgcaacat cccacgtttc cacaggcggc aggtcgagat
     3601 agtcgaactg ctcgccgcca tgcgccgcca aacggatttc cgtatcggtc aaacggctca
     3661 tctgttcata agcgatacgg ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac
     3721 cgtgtgccag attcatggct gccatattaa attgggttgt tgctccaccc tgcagaaaca
     3781 atatcttata gttgtcaggc actttcaaaa gctgcctcaa atcctgttcc gcatgatgca
     3841 ggatgctcaa aaacatttcc gaacggtggc tcatcgccat cacaggaaaa cccgtaccgt
     3901 tgtagtccaa catttcctgc cgcgccgttt ccaacacggc ttcgggcaat acggcagggc
     3961 cggcggaaaa attgtaaatc ggataaagag acataatgca gccttgattc tgaacaataa
     4021 cccgcccgat tttaggcttg cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg
     4081 cccgattcca tgccgcccga acacgggaaa caacatcaat atattgattt acaaatataa
     4141 aaatcatgct agcgacaaat agatacattt gttttgtcaa caatattcac gatttcccat
     4201 tacaaacctc ccttacaccc gcttttttcc gtcccaaaaa cacaaaataa atcaacactt
     4261 tcatttctcc gcaaaagcgg ttataatcac gccgattttt caactttgac gaaaaatggc
     4321 cacggggcca tttttttatt atgtacatag ggagcagcat ggatatccaa accatcctcg
     4381 aaaaaaccct gcccggcctg ggctacgaac tggtcgattt cgaactgacc gcgcaaggaa
     4441 cattgcgcgt gttcatcgat aaagaaggcg gcattaccgt cgaagactgc gcaaccgtca
     4501 gcaaccactt gagccgcgtc ttcatggtgg aagacatcga ctacaaaaac ctggaaattt
     4561 ccagccccgg actcgatcgc cccttgaaaa aagccgccga cttcgtgcgc tttgccggtc
     4621 agaatgccaa aatcaaaacc cgcctgccga tagatggtca gaaaaacttt atcggtaaaa
     4681 tcgaaggctg cgaaaacgat accgttacca tatccttcga cggcaaaacc gtacaaatcg
     4741 aattgggcaa catcgacaaa gcccgcctgc gccccgaatt caaattctaa aacacaacaa
     4801 tattggagat gttcaaaatg agtcgtgaaa tgttacagct ggcagaagca ctggcaagcg
     4861 aaaaaaacgt tgatgcggaa gtcgtcttcc aagcactgga attcgccctg tctaccgccg
     4921 ccaagaaaaa ggcagaccgc gaacacatgg atgtccgcgt acaaatcaac cgcgacaccg
     4981 gcgaatacca aaccttccgc cgttggctga ttgtcgccga tgaagactat acctatcccg
     5041 atgtcgaaaa aaccatcgag gaaatccaag aggaaattcc cggcaccgcc atccaaatcg
     5101 gcgaatacta tgaagagcag ctgcccaacg aaggcttcgg ccgccaagcc gcgcaaaccg
     5161 ccaaacaaat catcctgcaa cgcatccgcg atgccgagcg cgagcagaat ctgaacgagt
     5221 ttctcgccgt caaagaagac atcgtgtccg gcacggtcaa acgcgtcgaa cgccacggca
     5281 tcatcgtcga agtcgttgcc ggcaaactgg acgcgctgat tccgcgcgac caaatgattc
     5341 cgcgcgaaaa cttccgcagc ggcgaccgca tccgcgccct cttcctgcgc gtcgaagaaa
     5401 tcggcaacac cggccgcaaa caagtcatcc tgagccgtac ttccggcgat ttcctcgtca
     5461 aactgtacgc caatgaagta cctgaaattg cagacggcat gcttgaaatc cgcgctgtcg
     5521 cccgcgaccc gggacaacgt gccaaagtcg ccgtcaaagc caacgaccag cgcatcgatc
     5581 cgcaaggcac ctgtatcggc gttcgcggtt cgcgtgtcaa tgccgtcagc aacgaattgt
     5641 ccggcgagcg catcgacgtt gtcctttggt cgcccgagcc tgcccaattc gtcatgagcg
     5701 cgctctcacc cgccgaagtc agccgcatcg tcatcgacga agacaaacac gccgtcgatg
     5761 tcatcgttgc cgaagaccag ctcgcgctcg ccatcggacg cggcggtcaa aacgtgcgcc
     5821 ttgcttccga cctgaccggc tggcagctca acatcatgac ttccgccgag gcagacgaac
     5881 gcaatgcggc agaagatgcc gccatccgcc gcctgtttat ggatcacttg aacgtggacg
     5941 aagaaaccgc cgacgtactg gttcaggaag gttttgcaac cttggaagaa gtcgcctatg
     6001 ttcctgccgc cgaactgctt gccattgaag gatttgacga agaaatcgtc gatatgctcc
     6061 gcaaccgcgc ccgcgatgcc atcctgacca tggcgattgc cgccgaagaa aaactgggcg
     6121 aagtatccga cgatatgcgc aacctcgaag gcatagatgc cgatatgctc cgcagccttg
     6181 ccgaagcagg cattaccacc cgcgacgact tggcagagct tgctgtggac gaactgattg
     6241 aaatcaccgg tgtaaacgaa gaaaccgcaa aagccgtcat cctgaccgca cgcgaacact
     6301 ggtttaccga agacaaataa agggggtaca gatgagtaac acaaccgtag aacaatttgc
     6361 cgccgagctg aaacgccccg tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag
     6421 caaaaacagc ggcagcgatt ccctgacgct ggacgacaaa cagcttctga acgcctacct
     6481 gaccaagaaa aacggcagca acggcggcac catcagcatc cgccgcacca aaaccgaagt
     6541 cagcaccgtt gacggcgtaa aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc
     6601 ttctgccgaa gaattggcag cacaggtaaa agccgcccaa acccaagccg cacctgtccg
     6661 gccggagcag acggcagaag acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga
     6721 agcccgtgcc aaggcagaag cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa
     6781 agccaaacct gccgcgcaga aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc
     6841 ggaaaccaaa cccgccgaag aaagcaaagc ggaaaaagcc caagccgaca aaatgccgtc
     6901 taaaaaaccc gtcgagccca aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa
     6961 aggcaaagat gccaaaaaac cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt
     7021 cagcgcggaa gaacaggcgc aacgcgatga agaagcacgc cgtgccgccg cacttcgcgc
     7081 ccaccaggaa gccctgttga aagagaaaca ggaacgccag gcacgccgcg aagccatgaa
     7141 acaacaggca gaacaacagg caaaagccgc acaggaagcc aaaaccggca gacagcgtcc
     7201 cgccaaacct gccgagaaac cgcaggcagc cgcgccggcc gtcgaaaaca aacctgtcaa
     7261 tccggcaaaa gcgaaaaaag aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa
     7321 cgccaaaggc aaaggcggaa aaggcgggcg cgaccgcaac aatgcacgca acggcgacga
     7381 cgagcgcgta cgcggcagca aaaaaggcaa aaaactcaaa ctcgagccga atcaacacgc
     7441 cttccaagca ccgaccgaac ccgtcgttca tgaagttttg gttcccgaaa ccattaccgt
     7501 tgccgatttg gcgcacaaaa tggcggtcaa aggcgtggaa gtggtcaaag ccctgatgaa
     7561 gatgggcatg atggtaacca tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt
     7621 cgaagaactc ggacacatcg gcaaacccgc cgccgccgac gatcccgaag ccttcttgga
     7681 cgagggcgcg gaagcggtgg aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat
     7741 gggacacgtc gaccacggca aaacctcgct gctggactac atccgccgtg ccaaagtggt
     7801 acagggcgaa gcgggcggca ttacccaaca catcggtgca taccacgttg aaaccccacg
     7861 cggcgtgatt accttcttgg atactccggg tcacgaagcg tttaccgcca tgcgcgcgcg
     7921 cggcgcgaaa gcgaccgaca tcgtgattct cgtggttgcc gcagacgacg gtgtaatgcc
     7981 gcaaaccatc gaagccattg cacacgccaa agcggcaggc gttccgattg tggttgccgt
     8041 caacaaaatc gataaagaca ctgccaaccc cgaacgcatc cgccaggaac tgacccaaca
     8101 cgaagtgatc cccgatgatt ggggcggcac ggttcaattc atcgacgttt ctgcgaaaaa
     8161 aggaacaaac atcgacgcgc tgctcgaagc agtcttgctc gaagccgaag tattggaact
     8221 gaccgcacct gtcgatgcgc ccgccaaagg catcatcgtc gaggcgcgct tggacaaagg
     8281 ccgcggcgcg gttgccacat tgctggttca aagcggtacg ctgaaaaaag gcgacatgct
     8341 gctggccggt acggcattcg gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat
     8401 taccgaagcc ggcccgtcca tccccgtcga aatcctcggc ttgtccgacg taccgaatgc
     8461 gggtgaagac gcgatggtat tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg
     8521 ccaaggcaaa taccgcgacg tgcgccttgc caaacagcag gcggcgaagc tggaaaatat
     8581 gttcaacaat atgggcgaaa cccaggccca atctttgtcg gtcatcatca aggcagacgt
     8641 gcagggctct tacgaggctt tggcgggcag cctgaaaaaa ctgtccacag acgaagtgaa
     8701 agtgaacgtg ttgcacagcg gcgtgggcgg cattaccgaa tcggatgtca accttgccat
     8761 cgcttcgggc gcattcatta tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact
     8821 tgccgaaaat gaaaacgtgg aaatccgcta ctacaacatc atctatgatg ccatcgacga
     8881 cgtgaaagcg gcgatgagcg gtatgctttc tcccgaagag aaagaacagg ttaccggtac
     8941 ggtcgaaatc cgtcaggtta tctccgtttc caaagtcggc aatattgcag gctgtatggt
     9001 taccgacggc gtggtcaaac gcgattccca tgtccgcctc atccgcaaca acgtggttat
     9061 ccacacgggc gaactggctt cgttgaaacg ctataaagac gatgtaaaag aagtccgcat
     9121 gggcttcgag tgcggcctga tgattaaagg ctacaacgaa attatggaag gcgaccaact
     9181 cgaatgtttc gacatcgtcg aagtggcacg caccctgtga ttttgatgcg gaaaaatgcc
     9241 gtctgaagcg ttcagacggc atacgaaacg ggttctgtat catacagaac ccgttttttg
     9301 tcgcaaatcg gcttcagaca gccctcttgc cttatcccga tttgaatctg acttgccata
     9361 caaacaggct tcagacggca ttatagtgaa ttaaatttaa accggtacag cgttggctcg
     9421 ccttgccgta ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt aaatttaatt
     9481 cactatattt tcccgacagg cggatttgaa gtttctccac atatccggcg cgttcggcgc
     9541 ggctggtttc ggggcggtgc gtattgagcg acgaccattt ccaatgactg cgggctttgt
     9601 tgagttcggg cgggagtctg gcggcgtccc acggggcttt gcggctgtgc agctcgatat
     9661 ccgactgtgc cgcgtgtccg cgcgtttgca ggacgtggag caaatcgagg gcgcgggcgg
     9721 cgagcagggt caaggtttca gggtcggtgt gcagggtttg gcggccagcg agtttgtcgg
     9781 aaatggtgcg ggtattgggc aggatgccgc ccaaaaagcc gccgattgcc gtacccaatc
     9841 cgagcgagcc gccgagtgtg gcgatgtcca gccccaagcc gatgagcgcg ccagttgccg
     9901 cgcccgtgcc ggtgcggatg ccgtattgtt tgagcaattc gctgtcgaac gggtcttggc
     9961 ggaaggcttg cggcatccag tcgccgccgt cgatttcgct gtggtagaaa cggtaaaggg
    10021 caaacagccg ctgctgcatc tgccgttcga gttggcgtat ttccgcctgc atggtttgca
    10081 gcacggtggc ggtatcctcg ttttcgtcca cttcctgcct gaaggcggcg gcatcaatta
    10141 aaaagtcggc gatttcgcgg cgcgcttcgc cgtccagccg ctgccattcg cgccggcgca
    10201 tggctgtcag gcggtcaagt gtgctgcgtt cgggcaacat ggtggcgagg ttttcccaca
    10261 ggcgtagttc gccttcaaaa tcaaaggcga cggtgtcgaa ccctgcgaaa acgtgcaggt
    10321 ttctcctcgc cagcatggtt gtccacgatt cgggaggctg tccgccggta aagttgaaca
    10381 cgggcataac cggtttggca caccatgaaa ggatggtcaa ctcgtccctg tatttgtcga
    10441 ggacgggttc gcgcgcgtcg atgacgtaca ttgccatatc gctttgcaag acttgccgta
    10501 agactttggc ttcctgattg aaatcatggt gcgcgccgtg gctgccgaga aactgttgca
    10561 gccgttcgat gccgtctgaa cgattgtccg tatggttttc cagccattcc agcacgccgc
    10621 ccgcgtcttc gagtccgggc gtgtcgtaca ggaaaaccag cgtgtctgcg ccgtcgctga
    10681 tggcggcttc ttcgacatga cgcgtggtcg atggggcgtt tctgacttcg ccgaaaccgc
    10741 tgtcgcgcaa aagggtacga aggagcgagg ttttgccggt attggtatgc ccgacgacgg
    10801 cgagggaaag gggttgtttg ttcatgatgt ttttgaagaa tggattttca gacggtcttt
    10861 tttcagaatg gcggcttaac agaacatttc aagtgagttt attggtcttt caaacggcct
    10921 tcctgcgcca ccctgtcagg ctcaagccac gccgcgccgc attcggtcag cgcgttacgc
    10981 caatgttcca gcttttccgc aaggtcgtct gaaagcccct gttccgccag aagctgcacc
    11041 accgcgccgc cctgcgccgc ttccgagagc cggacaatct gccgcaacac gccccggtcc
    11101 ggcacagttt gggcgcgcac gccgataagc agttgcgccg gtttctgctt cagctctgtc
    11161 tccagcgcgg caacctgttc ccgattggcg gcaacgccct tatccagcca ttcctgcgcc
    11221 agcctgcccg taaaccatgt cccttcgtgc cactcggtct ccagcatgac cgcccatttc
    11281 ggcgcatcgt tcaagacgat tttcggcgaa acggcggaca cggtttcccg acgcgtatcc
    11341 gcatcggtaa ttttgttctg ccagcggcgg atgaccgcct gataataggg cttttccaaa
    11401 tccaatccgt tttcgcttgt tttcaaaagg attttgcata ccgcccaagc caagaggcgc
    11461 ggcaggatgc cgtagcaggc gatactgccg accagcagcc ccgaccaagc ccgcgcatcg
    11521 gcaatattgc cgttcagacg gccttcgatg accgcccgcg catcaggcac gggaaaaccc
    11581 agtttcgcag gcagccatgc caacatttcc accgcgcgta ccgaagcggc attgctcaac
    11641 agcgtgcttt cccagttgaa cgtatattgc cgcaccaaaa gcagcagcaa taccgacacc
    11701 agcattccga gcagcgtgca gagccacagg ctgtgcgacg ttgcgcctat tttccaacgt
    11761 accgaaggtt gccgccactc gtccgcatac agccgcaaca ccgcctgatt gacagggtct
    11821 ttgccccgaa accacgtcgc cggactgctg aaaaaacgcc ccactttcac gcgcaggaac
    11881 aacgttgcca accatactgc taacatcagc gtattcatgc ccaacacgcc cgccaaaatc
    11941 aggaagaaat tcagcccctg attgtccatc agaagataag tgcctgaaaa tccggcggta
    12001 aacatcatcg atgctgccac cacccacaac cagaacgacc ccacacgcac acgttccaac
    12061 gtgtcccgca gcatacggtt cctgtcgatc atctccgccc gacggatgat tttttcctcc
    12121 gtactgccgt ccacgcggcg caaagcctcc gtcgcctgca cgggatcgcc gctgaaaata
    12181 tagccgccct cttccaaaat gcgaaccaat tcaaccagtt tttgggaagg attcaacata
    12241 aaatgccatc tgaaaataaa aaaacagatt ttaacacacg cattttcaag aatattcaca
    12301 gtgtaggcaa agagtaaatc ccacacagag gcaaaagtat cggcgtaaac tgactgcctc
    12361 tactttcccg aaagattgtg cgatgtatac aggcgaacgc ttcaatactt acagccattt
    12421 gagcggtttg attctggcgg cggcaggttt ggcgctgatg ctgctgaaaa ccataggaca
    12481 cggggacggc taccgtatct tcagcgtatc gatttacggc atcagccttc ttctgctcta
    12541 tttgagttcc tcgctgtacc acggaattgc agccggaaaa ctgaaaagca ttttgaaaaa
    12601 aaccgaccac tgcatgattt atgtgctgat tgccggaagc tacacaccgt ttgcactggt
    12661 ttctttgaga aacgggccgg gctggacggt attttcactg tcctggctgc tggcggctgc
    12721 aggaatcgca caagaactca ccatcggacg gaaaagcgaa aaacgtctgc tgtctattgt
    12781 gatttatgtc gtcatgggtt ggatggtctt ggcggtaatg aaatccctga cagcctcact
    12841 cccaccggca ggactggctt ggctggcggc aggcggtatg ctgtacagtg tcggcattta
    12901 ctggtttgta aacgatgaaa aaatccgaca cgggcacgga atctggcatc tgttcgtatt
    12961 gggcggcagc atcacccaat ttgtcagcgt gtacggttat gtaatctgaa tgccgtctga
    13021 aaagcaaaac ctcccgttcc tgaagattgg gaggttttct ctttgccgga catcagccct
    13081 tgtcgtggaa ctcgtagaat tcatactgat aggacaaatc ctgtctcgcc tttttctgca
    13141 tcaggtagtc atcataaatg atgcggattt ccttacgcag caagaataag gcaatcaggt
    13201 tggggataac cataaagccg ttgaacatat cggacaggct ccaaaccaaa tcgactttgc
    13261 cgagcgtacc caaaacaatg gcaagcagaa ccaatgcgcg atagatgccc aagtgtcttc
    13321 ccctgaaaag aaaacggatg ttggactcgc cgaaataata ccaaccgata atggtcgtaa
    13381 aggcgaagaa ggtcagacac acggcaagca attgcgaacc gaagcccgga aatgccttgt
    13441 taaaggcaaa ttgagtaacc gccgcgccct gttcgcccga aaggttggca tcggtcagca
    13501 ggataatcaa tgcagttgcc gtacatacca aaatcgtatc gataaacaca ccgacaaatg
    13561 ccgccatacc ttgctgcaca ggatgcttca catccgcagt cgcgtgggcg tgcggagtcg
    13621 aacccatacc tgcttcgttg gaaaacagac cgcgcgccac gccgaaacgt atcgcttcgc
    13681 gcataccgat acccgcagca ccgcccaaaa cggcttcggg attgaaggcg gcggtaaaga
    13741 tgtggttgaa catcggcaca atatggtcgg aaaattcaaa caggataacg acggcgcaca
    13801 aaatataaac aaccgccata aacggcacga caaactgggc aatattggca atacggttca
    13861 cgccgccaat cacaaccatg cccgcaagga cggcaagcac aataccgact gccaaagaag
    13921 gcacatcaaa tgcaatggta acggcagaag cgatggagtt tgcctgagtc gcattgccga
    13981 taaagcccaa cgcgacaatc agcgcgatgg agaaaaagcc cgacaggaaa cgcgccgcgc
    14041 cgcgaccgat tttcggagtc agaccgtgcg taatataaaa cgccggcccg ccgatgtatt
    14101 tgccgtggct gacgacgcgg tatttctgcg ccagcagtgc ctccgcaaaa atcgtggaca
    14161 tccccaaaac ggcagaaagc cacatccaaa aaatcgcgcc cggcccgcct gcggtgatgg
    14221 cggtcgccac gccggcgacg ttgcccgtac cgatttgcgc ggatatggca actgccaacg
    14281 cctgaaactg cgataaagac ttgtcgtctt tatcgccttt ggcaaacaag ccgccgaata
    14341 tggatttgaa tcccgcgccc agcttggtaa tctgcggcgc accgagatac agcgtaaaaa
    14401 acaggccgat acccaaaagc gcgtaaatca gcaggtagtc ccaaaggaac cgattgactg
    14461 tacccaccag aacagacaat atattttcca taaaataaac cttatcttac aattaaaatg
    14521 actgccttcc aaaagacatt ccaataagga aacacggcga gcagaccgta tttgccgcaa
    14581 cagatgcctt aaattgtcaa caatcgggga gaagttgtgc catcataccg taaaatatcg
    14641 ttaattaaaa tatttctata tttttaagcg gaaagcggag gaaatcgctt tcagacggca
    14701 tagacaacgg cacggcataa aacaggatat tttgggtgct tgcaacttat gttaaaatgc
    14761 cgaccgtaaa aaatctgaca aaaacagatt aattatttga aataagaaag gaaatttatg
    14821 gcaggccata gcaagtgggc gaatatccag cataaaaaag cccgtcagga tgccaaacgc
    14881 ggcaaaatct tcacccgttt aatcaaagaa atcaccgttg cggcgcgtat gggcggcggc
    14941 gatcccggtg ccaacccgcg cctgcgcctg gctttggaaa aagcagccga aaacaatatg
    15001 cccaaagaca atgtgcaacg cgccatcgac aaaggcacgg gcaacttgga aggcgtggaa
    15061 tacatcgagt tgcgctacga aggctacggc atcggcggcg cggctttgat ggtggactgc
    15121 ctgaccgaca acaaaacccg caccgttgcg gacgtacgcc acgcgtttac caaaaacggc
    15181 ggcaacttgg gtaccgacgg ctgcgtggcg ttcaacttcg tgcatcaggg ctatttggta
    15241 tttgaacccg gcgttgacga agacgcgctg atggaagcgg ctttggaagc cggtgcggaa
    15301 gacgtggttg ccaacgacga cggttccatc gaagtcatta ccgcgccaaa cgattgggcg
    15361 ggcgtaaaat ccgctttgga ggcggcaggt tacaaatccg ttgacggcga cgttacgatg
    15421 cgcgcccaaa acgaaaccga actctctggc gacgatgccg tcaaaatgca aaaactgatt
    15481 gacgcgctgg aagacttgga cgacgtgcaa gacgtttaca cttccgccgt attgaatttg
    15541 gactgatacg cagcacagca gacatacaat aaaatgccgt ctgaaccttt cagacggcat
    15601 tgatttattt agcccttgcc cacgcccacc aaaccgtcag gacaaccgcc agaaaatacg
    15661 ccgcgctcca aacaggcaaa gcaattccca gcagataatc cggttcggca caatttccga
    15721 acccgcgcac gacaggctcg aaccaatcaa acaaaggcca gcctttcaag cggaacgtcc
    15781 acggcgcgcc gcatggagga gccgtacccg gcggcagcga ctgcagccac aactgatatg
    15841 ccgcaacaga aatacccgta acggccggaa tgctgataaa cactgcgcct gacagtccgc
    15901 ccaccctgcc cttgggctta cacgccagca caattaccgc acataatgcg gttgccaaga
    15961 cgcacaaccg ctgactgata cacaaaacgc aaggctcaat acccaaaaca tactgtgccg
    16021 ccaaagagcc ggcaaatgca cagaccgaaa cggcaaacag cagccaaacg gcttttctaa
    16081 ataacggggt cattttctca acacaccaat caaaataccg atatgccgat tttgctggat
    16141 atatcccgag atggcaaggg acaaaacggc gatttgcccg cacaatgccc acaaaaaata
    16201 catccggagg atttgaattt cagcaaattt agcgaatcaa aagtttattt caatgaaatc
    16261 atatgatttt tttgaataag cggattgatg ggtttttgaa ggaatttgtt accggatagc
    16321 catcgggcaa gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga
    16381 cagcggataa gaaacggcgg ataattcccg ccgtcgagtt gcttgatgca gcttgatttt
    16441 tctcctctat ttctcctttg tagacttggc acacattcaa ccggatgtgt gcattttttt
    16501 atctccgctt tttttgaaat ttaattactt ttattgtttg aaattccatt ctttaagaaa
    16561 tttaacaaga aaaagcttgc tttttatgac tgatagcggc ataatttgac ccgttagctg
    16621 gttcgagtag tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt
    16681 ctatcgaatg tgtgcatttt tttatctaaa acgacaacat gccatacgtt ttgttgttat
    16741 atttcattca ggtgtcaaac tgcatagcca gcctgaaata ttcaatccaa atccaaaacc
    16801 ggattttctt tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt
    16861 tgcaatagga tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta
    16921 atcagataat cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc
    16981 acttcttttt tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca
    17041 aaaaccaata aaggtttgcc caacagatgg ggattgagat gggcgtgata gccggaaata
    17101 taatgctccc gctccaaacg gcgtacccgt tcggtaactg gagtagtgga caatcctact
    17161 ttctcggcaa gctccgtcat cggaatacgg gcatttttct gcaggatctt aaggatacgg
    17221 aaatcaatct tatctaactc tttcatttgt ctttacctta ttgtttatta ttgattttaa
    17281 tggttgggat gctgataagg aaagacaatt atatcaggaa aacgcaagcc tcctttccct
    17341 atggcaaacc caaccccacc tccgaagagt aaggttcttt aatattttaa aagccttgcc
    17401 gttggattat aatcccccaa ccgatttctt aattttgcta ataaacactt gtttggtaag
    17461 gaatgaattt atgcgtcctt tgaacgtgca gatcaggttg ggcaacctta ggcacaatta
    17521 tcggattttg aaggaaatgc acggaggcaa gctgttggcg gtagtgaagg ccgacgcata
    17581 cggacacggt gcggtcagat gtgctttcgc gctggcagac ttggcagatg gtttcgccgt
    17641 ggccacaatc gatgagggaa tcaagctgcg ggagagcggc attacccatc cgattgtcct
    17701 tttggaaggc gtatttgaag catcagagta cgaagcggtc gaacaatact cgctctggtc
    17761 ggcagtcgga aaccaatggc aacttgaggc tttgctttcc cgtcattgga aaaaacctgt
    17821 caaggtctgg ttgaaaatgg attcgggaat gcaccgtacc ggttttttcc ctcatgatta
    17881 cgcttcggca tatgcggcat tgaagcagtc ggaatatgtg gacagtattg tcaaattctc
    17941 gcatttctcc tgtgcggacg aacccgaaag cggtataacg gaaatacaga tggaagcatt
    18001 cgatttgggt actgaagggc tggaaggcga agaaagcctt gccaactccg ccgctatttt
    18061 gaatgttccc gaagcacgca gggactgggg gcgagccggc ttggcgttgt acggcatttc
    18121 cccgttcgga ggcagcgatg acaggctgaa acccgtgatg aggctttcca cccgtatttt
    18181 cggcgaacgc gttttacagc cgcactcccc tatcggttat ggcgcaacat tttataccag
    18241 taaatctacg cgcgtcggcc tgattgcctg cggttatgcg gacggttatc cgcgccgcgc
    18301 cccaagcaat tcccccgtcg ccgtcgacgg taaattgacc cgggtcatcg gcagggtctc
    18361 tatggatatg atgaccatcg agctggacgc ttcgcaagaa ggtttgggac acgaggtcga
    18421 actgtggggc gatacggtta atatcaatac cgttgccgaa gcggccggaa ccatccctta
    18481 cgaattgatg tgcaatatca aacgtgcaaa attcacttat atcgagtaat caagtccaaa
    18541 caaaaatgcc gtctgaagcc tttcagacgg cataaatgcg ttttgacaaa atcagtatag
    18601 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
    18661 ggcgaggcaa cgctgtactg gtttaaattt aggggctgtc ctggataact aggataaact
    18721 tgattttact aattgtttta aaatggaaca agaactttta tctcactgtt gttaaaacgc
    18781 cattcgcact cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt
    18841 aaatgacgtt ttgcctggtt ccaaaagttc tcaattccat taatatggtt ttgtcgttcg
    18901 gcaaaatgtg tgctgtgatt gatacgaaaa cgaagtttca gcgaagctaa aatggctaaa
    18961 ttcgctcaca tctaatacat catagctacg ataacaatcc gtataaacaa tgctgtcagg
    19021 tttcacttgt tcacggataa taggaaataa agtagcggtt tgagtattcg gtactgtaac
    19081 cgtataaacc ttaccatttc gcttcaaaag accgaatacg gcgactttac cggcagcacc
    19141 gccaccgcgt ttgcctttgc gttgtccgcc aaaataactt tcatctgctt ctacttcgcc
    19201 atcaaacatt tccaaatgcg gactgttttg ataaataagt aatcgtaaac gatgaaaata
    19261 ataggctgcg gtatttttat taacgcctac taactctgct gccgttcttg cagttacacc
    19321 tgcgacaaat agctcaatga gtttattttg tttatactgg cttagacgac tttttctcat
    19381 agggataatt ctaacttaat ttgaatttcc ctagttatct aggacagccc catattcttt
    19441 tctaaagaca ttttacccga tgcctataaa caaaaatgcc gtctgaagcc tttcagacgg
    19501 catttcccca tcaaaaccgc aatcagtttt tcatcgattg aaccggtgcg ggaatcctgc
    19561 cgccccggtt gacgaacact tcgcatgagc cttcttttac cggcattaca ggcgcgtagc
    19621 ccaacaggcc gccgaactcg acgctgtcgc cgacggtttt accggttacc ggaataatgc
    19681 gcacggcagt ggttttgctg ttgatcatgc cgatggcggc ttcgtcggca atgatgccgg
    19741 aaatggtgtg cgcgggtgtg tcgccgggaa cggcaatcat atccaagccg accgaacaaa
    19801 cggcggtcat cgcttcgagt ttatccaacg tcagcacgcc tgcttcggcg gcggcaatca
    19861 taccttcgtc ttcggaaacg gggataaacg cgccactcaa accgccaacc gcgctcgaag
    19921 ccatcatgcc gccctttttc acggcatcgt tcagcaatgc caaagctgct gttgtgccgt
    19981 gcgtaccgca gacgctcaaa cccatttctt caagaatgcg cgccaccgag tcgccgacgg
    20041 caggggtcgg tgccagcgac aagtcgagaa taccaaacgg gatattcagc atttttgagg
    20101 cttcgcggcc gatgagttcg cccacgcggg taattttgaa ggcggttttc ttcacaactt
    20161 cggcaacttc ggtcaatgtc gttgcatccg aattttccaa cgcggctttt acgacacccg
    20221 ggccggatac gccgacatta atcacagcat ccgcttcgcc tgagccgtga aacgcgcccg
    20281 ccataaacgg gttgtcttcc accgcgttgc agaacacgac gattttggcg cagccgaaac
    20341 cttctagtgt gatttcagcc gtgcgtttga tggtttcgcc cgccagtctg accgcgtcca
    20401 tattgatacc ggcgcgcgta ctgccgatat tgatggagct gcacacgata tcagtagtct
    20461 tcatcgcttc gggaatggaa cggattaaca cctcgtcaga aggcgacata cctttttgca
    20521 ccagcgcgga aaagccgcca ataaaagaca cgccgatggc tttggcagcc ttatccaaag
    20581 tttgcgccac gctgacgtaa gaatcagcat gggtggccgc cgcgatttgg gcaatcggcg
    20641 tgacagaaat gcgctgattc acaatcggca cgccgtattt ggcagacaga tattttgctg
    20701 tcgccaccaa gtctttgccg accgtggtaa ttttgttgta aatattttgg ttcaacacgt
    20761 cgatgtcggt gctgatgcag tcgtgcaaat caatgccgat ggtaatggtg cggacatcga
    20821 aattctggtc ggcaaccatt ttgacggttt ctaaaatttc gccggattgg atactcatca
    20881 cattcctccg gctcaaatgc ggtgcatcgc ttggaagatt tcttcgtttt gcatacggat
    20941 gtcgagcgcg agttttttgc tctcttccgc aaacaaatcc aaaacctcct gccgcgattt
    21001 gctgcatttt gaagtgtcca ccaagataat catggtaaaa aaatcatcca tcagctgttg
    21061 gctgatattg aggatgttga ttcggttttc cgccaaaatt ttggaaacat cgtacacgat
    21121 gccgacgcgg tctttaccga tgacggtgat gactgaattg ttcacaggct tactccttgc
    21181 agatatccgt taaagtccga aattatacca ccgttggatt ttgaagaaat attgtcaaca
    21241 atatatacat acaaaatgcc gtctgaaact atttcagaca gcatcaagat tcagggttcg
    21301 attaaataac catccttatc ccactgggtt ttcctgacca acttatcatc ctgataaaca
    21361 gcttcactct ttttagaacc atcttcatac cactctaaga ctactccatt acgtttatga
    21421 tgatggatag acagttccga gagtaatcga ccgctttcat cccatgtcag aattttggca
    21481 ggctcatcat tgaccataac catttccgtt ttgatattgc cgtcggcata ccattgcttc
    21541 catacgccgt ttgccttatt ctgtttaaac tggatttcgc tttccttgcc gccgttacgg
    21601 taatagcggt accccgtacc ttcactcaaa ccatttttat aaggcataac ggcagatttt
    21661 ttaccgttcg gataccagtt gacccactcc ccgtccggct tacccttgct gaagcccccc
    21721 gccatttttt tctgaccatt aaaatgccac aaaatcaaca taccgttttg cagggtaggc
    21781 acaaaagatt tgatttgcgt tgaagcaacg atataaggtt cggaatattt cttcatcgac
    21841 ggataataaa aatcctgcgc gtgcgcaata ccctccgcca cactatattg cctgatataa
    21901 gcggcagaag acatcgtcgc cgtcagtttc ccgttctgat taaaataaac agaataggtc
    21961 tgcgccggca aagcggccga aaaacccaac aggacagttg aaaatacaat ccgagataat
    22021 tttttcattg caatagcgat ataaaaacaa ggctgtgttt tagtaatctg ttgatttcaa
    22081 ttatttgcaa gggaaaagac aattattttc cggttaggaa taaacctatt ctattgaata
    22141 gattgaagcc aagtacgcct atcaacacta tattaaaaca ctgccaaaaa caattaactt
    22201 ataaacaata tggtaaggat ttctctgcca agcatcaaac ccgagacaac gtatcgtaaa
    22261 aatgccgtct gaaaacaaat cgtcttcaga cggcatttcc ctttcaactc actcttcacc
    22321 caataactgc tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca
    22381 agtaaaaggc aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac
    22441 cagcaataca cctttgggat tcagaaactt tgccgcattc agaagaatct gcctggtggc
    22501 atccaacccg tccgccccgc tgcccaatgc caattccggt tcgtgcaaat actcttcagg
    22561 caataactca accgattccg catccacata aggcggattg gaaacaatca aatcataagt
    22621 gccttccaat ccttcaaaca aatccgtatg aataagccgg atgcgttctt ccaaaccata
    22681 atcttcgaca ttaatccctg ccacttccaa agcatccaag ctcacatcaa ccgcatcaat
    22741 ttgggcatca ggataatgat gcgccatctg aatggcaagg caaccgcttc cggtgcaaag
    22801 atccaaagca ttatgcacca actcatcgta ttctatccaa gggcgaagtc cgtcacccaa
    22861 caattcataa ataaaagaac gaggtatgat tacgcgctca tccacataga aatcaaactc
    22921 tccctgccat gcctggtgtg tcaaataagc ggctggaatg tgttcgacag cacgacgctc
    22981 aataaccgcc agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc
    23041 aagcatatcc aaaggcaaat tcaaagtatg cagaatcaaa taagccgctt catcatgcgc
    23101 attatctgtt ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg
    23161 taaaatatcg cggatagtcg tcaattcttg tgctgcctga ttaaacataa tatgaaccat
    23221 tctgcgtata gatactttta attataacag aaacaacaag caaacctttt catatcgcca
    23281 aataaccacc caatctaccc atacaactac ataaatgccc gagcgaaaac catcgcccga
    23341 acggaaacga caatggccga cggtatgggc aatctgattg gctgggaaaa aacggggctt
    23401 gttgccggta agcagtggat aaccgcaaaa gacgacaagg tgtccgatgt ctgcaatgcc
    23461 aacggcgaga tgggcgtaat cgggctttac gagcctttct cacacggcac attgacgata
    23521 cccggtcatc cgaactgccg atgcgaggtt gtttccgtat cgggtggcga attgggggaa
    23581 tttgccgaaa aaaaggagct tcgtaaagcg gctatgcagt atgcgcggga taactttatc
    23641 ggcaaaagct atgtcaatga aaacagcggg catgaactga aggtaacttg gcaaggtgtg
    23701 aaacacgcta cgtcaaaggc aaatcaggcg gaattatcca tcatgacaaa acttgatgac
    23761 ttattgcgct acgcaaaata tgagggttct tattcggata ggaaaggtca tcctgatatt
    23821 attgcagcac ataagtatcg tgccgttgcc aaggttggga atgagtcttt aaatatcggt
    23881 gtgattgtaa gggaatttcc agacggacat aaacattacg accatttcat cttgaaggat
    23941 gagtaaagcc cttttgcagt gtcgttctgg agcggatagc gttaaggcaa gtacgcttcc
    24001 agccttacaa aagggcttta aattcagcat gccatttata caggcaggag taaacccatg
    24061 acaaagttat acgcagaaat cgccaagatg gagacgcagg acgacggcac ggtcaaggtt
    24121 tggggttacg cttcaagcga ggaaatcgat tcggacggcg aagtcatcgc ggcggcagct
    24181 atgaaggcgg cgattcccga ttatatgaag tttggcgcgg ggcgcgagat gcacggctca
    24241 aacgctgcgg gaacggcaat tgaaatcaac gttgaagacg acggcataac ctttttcggt
    24301 gctcatatca tcgaccccgt tgtcgtgtcg aaagtcaaaa caggcgttta caaaggcttt
    24361 tccatcggcg gcagcgttac cgcccgcgat gatttgaaca agtcgcaaat cacgggcttg
    24421 aagctgacag aaatcagcct tatcgaccgc cctgccaatc ccgatgcggt gtttacctgc
    24481 tttaaggcgg acaaaggtgc ggaagcggta aacaacgata cagaacataa tgctacatat
    24541 tttagccatt tcccttccaa acaaaaaaca aaaaagcacc ggcggcagcc gatgcccctt
    24601 cctttacagg ttcccctatt ttttcactgc gggcagcact ggtttggctg ggccttttgg
    24661 tgcgggcgcg ccgacggaag cctgatcctt cagcttcgcc agcaccgcag ggccgatgcc
    24721 ctttaccttg gtcaaatcgt c
//
LOCUS       DAWFKN010000024        22570 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFKN010000024 DAWFKN010000000
VERSION     DAWFKN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.024"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..808
                     /locus_tag="WGR46_000956"
     CDS             <1..808
                     /locus_tag="WGR46_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0855919.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMLDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKVIRGNV"
     gene            849..1379
                     /locus_tag="WGR46_000957"
     CDS             849..1379
                     /locus_tag="WGR46_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0855920.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1390..1529)
                     /locus_tag="WGR46_000958"
                     /pseudo
     CDS             complement(1390..1529)
                     /locus_tag="WGR46_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1548..3143)
                     /locus_tag="WGR46_000959"
     CDS             complement(1548..3143)
                     /locus_tag="WGR46_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0855921.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WGR46_000960"
     CDS             complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WGR46_000960"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0855922.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WGR46_000961"
     CDS             complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WGR46_000961"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0855923.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WGR46_000962"
     CDS             complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WGR46_000962"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0855924.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WGR46_000963"
     CDS             complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WGR46_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0855925.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WGR46_000964"
     CDS             complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WGR46_000964"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0855926.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WGR46_000965"
     CDS             complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WGR46_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0855927.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WGR46_000966"
     CDS             complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WGR46_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0855928.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLNLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAVLTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WGR46_000967"
     CDS             complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WGR46_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0855929.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11592..12061)
                     /locus_tag="WGR46_000968"
                     /pseudo
     CDS             complement(11592..12061)
                     /locus_tag="WGR46_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12118..13494)
                     /gene="argH"
                     /locus_tag="WGR46_000969"
     CDS             complement(12118..13494)
                     /gene="argH"
                     /locus_tag="WGR46_000969"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0855930.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGSAPEQVRFQV
                     KRWREMSA"
     gene            complement(13513..14382)
                     /gene="galU"
                     /locus_tag="WGR46_000970"
     CDS             complement(13513..14382)
                     /gene="galU"
                     /locus_tag="WGR46_000970"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0855931.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14410..15009)
                     /gene="rdgB"
                     /locus_tag="WGR46_000971"
     CDS             complement(14410..15009)
                     /gene="rdgB"
                     /locus_tag="WGR46_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0855932.1"
                     /translation="MSEKTEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYSTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVLHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(15002..15235)
                     /locus_tag="WGR46_000972"
     CDS             complement(15002..15235)
                     /locus_tag="WGR46_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855933.1"
                     /translation="MNRRKIYLLSIALFTLAFMLLVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15343..15876)
                     /locus_tag="WGR46_000973"
     CDS             complement(15343..15876)
                     /locus_tag="WGR46_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0855934.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15978..16436)
                     /gene="nudB"
                     /locus_tag="WGR46_000974"
     CDS             complement(15978..16436)
                     /gene="nudB"
                     /locus_tag="WGR46_000974"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0855935.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLAEGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16647..16723
                     /locus_tag="WGR46_000975"
     tRNA            16647..16723
                     /locus_tag="WGR46_000975"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16681..16683,aa:Pro,seq:ggg)
     gene            16881..18767
                     /gene="mafB"
                     /locus_tag="WGR46_000976"
     CDS             16881..18767
                     /gene="mafB"
                     /locus_tag="WGR46_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0855936.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            18798..19217
                     /locus_tag="WGR46_000977"
     CDS             18798..19217
                     /locus_tag="WGR46_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855937.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            19254..20228
                     /gene="mafB"
                     /locus_tag="WGR46_000978"
                     /pseudo
     CDS             19254..20228
                     /gene="mafB"
                     /locus_tag="WGR46_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            20239..20700
                     /locus_tag="WGR46_000979"
     CDS             20239..20700
                     /locus_tag="WGR46_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855938.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            20726..20974
                     /locus_tag="WGR46_000980"
     CDS             20726..20974
                     /locus_tag="WGR46_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0855939.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            21001..21420
                     /locus_tag="WGR46_000981"
     CDS             21001..21420
                     /locus_tag="WGR46_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855940.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            21498..21581
                     /locus_tag="WGR46_000982"
                     /pseudo
     CDS             21498..21581
                     /locus_tag="WGR46_000982"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21578..21820
                     /locus_tag="WGR46_000983"
     CDS             21578..21820
                     /locus_tag="WGR46_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0855941.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            21898..22143
                     /locus_tag="WGR46_000984"
     CDS             21898..22143
                     /locus_tag="WGR46_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0855942.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            22146..22535
                     /locus_tag="WGR46_000985"
     CDS             22146..22535
                     /locus_tag="WGR46_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855943.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5376 a         5084 c         6433 g         5677 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcagatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgctgga
      181 tgtggtgttt ttaaatgcag ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttaa tagggatata tcaagttatc tttattcctt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcagaat
      781 tttaaaagta atcagaggga atgtttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaaggat
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gaatcggcaa ccgcgtcttg gtcggtatgg gcagcaccgt tttggacgat gctgtgattg
     1201 aggacgaagt gatgattggc gcgggcagcc tcgttccgcc gcgcaaacgc ttggcgggcg
     1261 gctaccttta tatcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg acgggatgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaagcccttt tttcgggttt agacggcatt ttatcgccga ttgcttttta cagtttgacc
     1561 gaatgttcgc gcgtttcgtg gaacacgatg tccggccaac gttcttgcgt gagtcccaga
     1621 ttcacgcggt taggggcgag gtaggcgagg ttgccgcccg cgtcgatggc gaggttgccc
     1681 gcgttggctt tttcaaattc cgccagtttt ttcttgtcgt cgcacgatac ccagcgcgcc
     1741 gaccagatgg atgcgctgtc gaacacggct tctacgccgt attcgttggc cagccgcgag
     1801 gtaacgactt caaattgcaa cacgccgacc gcgcccaaaa tcaaatccgc gccgctcatc
     1861 ggtttgaaaa cctgtaccgc gccttcttcg ccgagttgtt gcaaaccttt ttgcagttgc
     1921 ttgattttca gcgggttttt gatgcggacg ctgcggaaca gttcgggcgc gaagaatggg
     1981 atgccggtaa acgtcagttg ttcgccttcg gagaagctgt cgccgatttg gatgttgccg
     2041 tggtttggga taccgataat gtcgccggcg taggcttctt caaccagctc gcggtcgtgg
     2101 gacatgaagg ttaccacgct ggaggcggcg atttcgcggt tgatacgcag gtgtttcatc
     2161 ttcataccgc gctcgaatct gccggagcag acgcgcaaga aggcgatacg gtcgcggtgt
     2221 ttcggatcca tattggcttg gattttaaag ataaatccgg aaaacttagc ctcgtccggc
     2281 tcgaccatac gtacggtcgc gtcgcgcggt ttcggcgcgg gcgcccagtc aattaatgaa
     2341 ttgaggattt cctgaatacc gaagttgtta atcgcagagc cgaagaatac gggcgtgagt
     2401 tcgccggcga ggaattcgtc gagattaaac tcgttcgatg ctgcctgcac caattcgatt
     2461 tcgtcgcgca actgttggat ttccaatgga aagcgttgtt ccaattcggg attttctatg
     2521 cctttgatga tgtcgaactc gtgcggcagg cgttcgccgc ccgcttcaaa gagatagatt
     2581 tcgtcgttca ggatgtggta tacgcctttg aagtttttgc ccataccgat cggccaggtg
     2641 acgggcgcgc agcggatttg caggatgttt tccacttcgt ccagcaattc cagagaatcg
     2701 cgtacttctc ggtcgtattt gttcatgaac gtaacaatcg gcgtatcgcg caggcggcag
     2761 acgtttaaga gcttgatggt ttgcgcttcc acgccttttg ccgcgtcgat gaccatcaac
     2821 gcgctgtcca cggcggtcaa aacgcggtag gtgtcttctg agaaatcttg gtgtcccggc
     2881 gtgtccaaga ggttgacggt gtggtcttta tagtcgaact gcatcacgct tgatgccacg
     2941 gaaatgccgc gctgcttctc gatgtccatc cagtcggaag tggcgaattt gccggttttt
     3001 ttgcctttta ccgtacccgc gctttgaatc gcacctgaaa acagcaagag tttttcagtc
     3061 aacgtggttt tacctgcgtc agggtgggag atgatggcaa acgtgcggcg gcggcgcact
     3121 tggtcgagga tttcttggga catggttttc tttgcaaaaa ggttcaggcc gcttttcaga
     3181 cggcccggac agtgtttgag acggcgaaat tgtacaaaaa aatgcctgat aattcaatgt
     3241 tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg tctttcagga cggcatcggc
     3301 ggtttcccac gcgctgccac gccagacttt gtaaaagcag ctttctcgcc cggtgtggca
     3361 ggcgatgccg ccgttttggg cgatgagcat cacaatggcg tcgccgtcgc agtcgaggcg
     3421 cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc ttcatccatt gtttttggcg
     3481 cgaacggctg taatagtggg caaagccggt ttcgacggtt ttttgcaggg cttcggcgtt
     3541 catccacgcc accattaaaa tacgtttggt ttcggcatct tgggcgatgg cgcaaaccaa
     3601 acctttttcg tcaaatttga cggcttcaag caggttttta tccatatttc ctttcagacg
     3661 gcatagtcga ggcggtcaga ggcgcacttc gatgccggct tcgcgcatag cgcgtttggc
     3721 ttcgcggatg gcgatttccc cgaaatggaa aacgccggcg gcaagtacgg catcggcttt
     3781 gccttcggtt atgccttcaa tcaggtgccg gacattgccg accccgccgg aggcgatgac
     3841 ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc aggttgaaac cctgtttcgt
     3901 accgtccctg tccataccgg taagcaggat ttcgcccgcg ccgcgttttt gcatttcgat
     3961 cgcccattcc accgcatcca aaccggtcgg attgcgcccg ccgtgggtaa agatttccca
     4021 gcgtgtgttt ccggggttga cggctttggc atcgacggcg gcgacgatgg cttgcgaacc
     4081 gaaaaatccg gcggcttcgt caatcaggtc gggacgggta acggcggcgg tgttgatgct
     4141 gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg gcaacggtgc gtacgccgcc
     4201 gccgacggtc agggggatga agacttgtcc ggcaacctct tcgatgatgt gcaggatggt
     4261 gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc aattcgtccg cgccttcgcc
     4321 gttgtagcgt ttggcggctt cgacggggtc gcccgcgtcg cgcaaaccga tgaagttcac
     4381 gcctttgacg acgcgcccgt cttttacgtc gagacagggg atgatgcgtt ttgccagtgc
     4441 cataatcgga tgcctttagt cgagggaatc tgccagttgc tgcgcttggg caaaatcgat
     4501 gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct acgccatgtt tttcggcggc
     4561 acacagggcg cggatgtcgt ccaagccggt cagtccgccg gaggcgatga cgggaatgcg
     4621 gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg ccgctcatca taccgtcgcg
     4681 cccgatgtcg gtgtagatga tgctgttgac accgtcgtct tcaaagcgtt ttgccaaatc
     4741 aattacatga tgcccggtta cggttgccca gccgtcgatg gcggccatac cgtctttggc
     4801 atccagcccg acgataatcc tgccggggaa ggctttgcac gcctcgcgca cgaagtcggg
     4861 gtttttgact gccgccgtgc cgataatcac gtcgtttaag cccaaatcca aatattgtcc
     4921 gatggttttc aaatcgcgta tgccgccgcc gagctgtacg gggatgtctt tggcgacggc
     4981 ggcaaggatg tctttgatgg cgggcaggtt ttgcggaaca ccggcaaacg cgccgttcaa
     5041 atctaccaga tgcaggcggc gcgcgccttg tttgaaccag tgcagcgcgg tttcggcggg
     5101 cgaatcggaa aagacggtcg cctcttccat cagcccttgt ttcaggcgga cgcagcgtcc
     5161 ttctttcaaa tcgatggcgg gtatcagcag cataattttt ctccttgtgc ggggccgtgt
     5221 ccggcttacc agtttaaaaa gtttttcaac atcgtcagcc cggcatcgtg gcttttttcg
     5281 gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga tgcaggcaaa cggggacggg
     5341 tagtcgcttt cgcccaatat ggtttcggga ttttcggggg cgaaatagta gctgtggacg
     5401 aagtaaaaac gcgtgtcttg gggaatatct ttaaacagcg ggtggttttg ggtttggcgc
     5461 acggtgttcc agcccatatg cgggactttc agacggcatc cctgcgggtc gcggaggtcg
     5521 cgctcaaagc gtctgacttt gccgccgaac cagcccaagc cgtcggtgtt tccttcttca
     5581 ctgtggtcga ataaaagttg cgcgccgacg cagattccga aaaagggttt gttttttaag
     5641 gcatctttga ctgcctcgtc caggccgccg cgcgtcagtg ccgccataca gtcgggcatc
     5701 gcgccctgcc cgggaaaaat gactttgtcg gcgcgggaca cgcgttcggg gtcgccgctt
     5761 aaaaagattt cggtattttt tccggcaagc tgccccgccg tccggacgga tttcaatacg
     5821 gaatgcaggt tgcccatacc gtaatcgata atggcggttt gcatggcttc ctcctctttt
     5881 tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa atgccgtctg
     5941 aaaacgggag tctgtcttca gacggcatag ggtttaaacc cggaaagccg tttgtcagcc
     6001 ttccatttgt tttgcctgaa cggcagtcag ggctatggtg aacacgatgt cttccaccag
     6061 cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct
     6121 taagacgttg gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg
     6181 gaagaccaaa acggttgcct gtcctgccac cgggctgccc ggggctttgg atttgcccac
     6241 gcccggcacg gttgccgcat catattgcag cgggccgtcg atggcgaggt ctgggcgttt
     6301 ttcccgggca agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt
     6361 gacggtggag taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc
     6421 ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca
     6481 gtcgccgaag acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct
     6541 tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg
     6601 aaccgcaccg gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac
     6661 cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag
     6721 ttcgcacatc ggtccgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga
     6781 gtcgggcagg ctgataccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag
     6841 caggacgcag cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg
     6901 ttctgcgcct tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt
     6961 gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc
     7021 tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc
     7081 aacggaggct gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa
     7141 gaactgtttg gcaaggttca cccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg
     7201 gacggcgaag acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag
     7261 gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg aggttggcgg catcgagtgc
     7321 ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt
     7381 ttcggcggtt gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg caaaggcttg
     7441 tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat
     7501 atatcctttc gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg catataggca
     7561 gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat
     7621 agcaaacaaa ttctgtttcc aacaagataa atatccgcag gtttgtggat gctgccgcct
     7681 ttcagagggt atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa
     7741 tgccgtctga cagggtgggg gcggaaggga ggttgagcgt gaggacggtt tgtccgaccc
     7801 ggagactgat ttcggtatgc cgcgctttgg gcgtggtttt gagaaccacg gcgtgaatgg
     7861 aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc cggacggatg agcagtgtgc
     7921 cgcgtgtgcc tgcgggtgcg ccgctttgaa cgggcaggcg gcccaatccg caatcggcgg
     7981 tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat
     8041 caaggtcggc aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct
     8101 gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga
     8161 aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc
     8221 gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg
     8281 cgagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc
     8341 gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt
     8401 gcgccgtctt gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt
     8461 gggggaacag cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt
     8521 tggtattttt cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt
     8581 caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa
     8641 agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt taaaaccggg gtgttttgaa
     8701 aacttttgga caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg
     8761 ttatttgaaa gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat
     8821 cagcgcgtaa ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt
     8881 ggagagtgtg tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag
     8941 tttgaggaag acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa
     9001 cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt
     9061 tgggagttgt tccaaggaag tccttagggt ggtttgcgcc atcggcaggt aaagcatgaa
     9121 ataggcaagg atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa
     9181 ataaaccaag gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct
     9241 gtctatccaa acggttaaaa aattgcgata gcggaccgat gcccaaacaa ggggcaaggc
     9301 acataatata gtcaaaaccg cacctaaagc cgatacgctt aaggaacgga taaaggcatc
     9361 aaatacggat acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac
     9421 gccaaagggg ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccaat
     9481 ctgaccgggg aggttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg
     9541 gtaaatcttg gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc
     9601 cattaaaaca gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg
     9661 gaaaatggcg gtggtaaaag tggggtagtt caaaatggat accgcgccaa attcgaccag
     9721 catatgcagg gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat
     9781 ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga
     9841 caggctgatg cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag
     9901 gctcataatc atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt
     9961 gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt
    10021 tttgccgaag aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga
    10081 aatcagggta acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg
    10141 gcggaacaac agttcgacgg cgcggttgat gccgacctgc cacgaacgca tagcgacata
    10201 taaaaaaggc agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg
    10261 tattttttta ggagacatag tgtttttcat cggcaaaacg ggcggacagt ataaatgtcc
    10321 acccgtttga caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg
    10381 cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg
    10441 gttccaaatt gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg
    10501 tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct
    10561 tgttttggga ggattttaac acggctgcgc cggaataggt aatgagtgcg ccgggatctc
    10621 tgtggcggac gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcgaaag
    10681 cgtgccagta gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa
    10741 gggcgacgga gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca
    10801 atgcggccgc ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg
    10861 aagtgggggc gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt
    10921 tttccaaatc tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc
    10981 tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga
    11041 tggtggaggc gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga
    11101 tttgttcgga atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg
    11161 caagctggtc gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg
    11221 catctgcaac ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca
    11281 gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca
    11341 tatcgatttt ctcctaatga atgagagtgt acaccttgtt aaaacataac ggtgtgtagt
    11401 gtattccttc ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc
    11461 ggttatggtt tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag gcggaagcgg
    11521 cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg aatgcttgag
    11581 aacccctctc tttaaaacag cgtctgaaca gggttgccgg aatagtattg ccatcccggc
    11641 agatacagtt tgtcggggtc ttgccaatat tgttcatcca gactgttcgg cagtgaggcg
    11701 gttttgttgt cgagatgttt tgttatatcc cacatttctt tcaggttttt accttccgat
    11761 tggaggtggc ggatttcttt tctcaatgcg tctgccgccc gatggatcag cattgcgctg
    11821 tgtatcgcgc cgattttttc agaacggaac aagtcttttc ggcggcggga aaaccccaat
    11881 tgcagacaaa ttgcagtatg ccgccgttgc cgatgtcggc ttccgcccgc caaatcaaaa
    11941 ccagctcctg ttcacgtccg tccatgcttt cgagtttgcc gtcatgctgt tcaaaaagtt
    12001 tgtctaccgc ctgccacatc atctgttcga agcggtcggc ttgggtatcc gtatcggtca
    12061 tggtgttctt gtcttttaaa aatgccgtct gaacatttct tcagacggca tcgggggtta
    12121 agctgacatt tcccgccagc gtttgacttg gaaacggact tgttcaggcg cgctaccgcc
    12181 caagtggttg cgcgcgttta agctgccttc gggtgtcagc acgccgtaaa cgtcgtcggc
    12241 aatcaaatcg ctgaaacctt gtaagacttc gagcggcagt tcgctcaaat cgacgccggc
    12301 ttcatcagca tggcgcacgg cttgggcgac gacttcgtgg gcatcgcgga aaggcatgcc
    12361 tttttttacc aagtaatctg ccaaatcggt ggcggtagcg aagccctgca tcacggcggc
    12421 gcgcatattg tcgggtttga cggttacgcc gcgcatcata tcggcgtaaa tccgcaacgt
    12481 gtcgataagc gtgtcggcgg tgtcgaacaa gggttctttg tcttcctgat tgtctttgtt
    12541 gtacgccaag ggttgggatt tcatcagggt aatcagaccg ataaggtgtc cgatgacgcg
    12601 gccggatttg ccgcgcacga gttcgggcac gtcgggattt ttcttctgcg gcatgatgga
    12661 cgaacctgtg cagaaacggt cggcgatgtc gataaagccg aaacgcggac tcatccacaa
    12721 aatcaattct tcggacaggc ggctcaggtg aaccataatc agcgaggcgg cggcggtgaa
    12781 ctcgacggca aaatcgcggt cggatacggc atcgagcgag ttttggcaga tttgctcgaa
    12841 acccagtaat tcggcggtaa tttcgcgccg aatcgggtag gtcgtaccgg caagggcggc
    12901 tgcgccgagc ggcatacggt tgacgcggcg gcggcagtcc gccatccgtt cgttatcgcg
    12961 gccgagcatt tcgacgtagg cgagcatatg gtgtccgaag ctgacgggct gggcgacttg
    13021 caggtgggta aagccgggca tgacggtttc ggcgttttgt tccgccaaat ccagcaatgc
    13081 cgtctgaagg ctttgaatca ggctttggat aacggtaatc tggtcgcgca gccaaaggcg
    13141 gatgtcggtg gcgacttggt cgttgcggct gcggccggtg tgcaggcgtt tgcctgcatc
    13201 gccgattttg tcggtcaggc ggcgttcgat gttcatgtgg acatcttcca aatcggacga
    13261 ccattcgatt ttgccgctgc ggatttcttc gaggatttcc gccatgcccc gacggatgtc
    13321 cgccaaatcg ccttcgtcca acacgccggt ttctttcaac atttgcgcgt gtgccagcga
    13381 gccttgaatg tcccattcgg caagccgtcg gtcgaaaccg atggaggcgg tgtattgttt
    13441 gacgagttcg gaaacgggtt cgttgaaacg tccggaccag gttttgtcgt gcataaggat
    13501 tccttgatgg ggttattcgg tgcggtattt ttccaaaagg cggcggaagg gttcgccggt
    13561 ttcggggtgt ttcaggccgt aggcgacggt ggcttcgagg tagcccagtt tgctgccgca
    13621 gtcgtagcgc gtgccttcaa aggggtgcgc caggacaaat tcgtgatcga gcagcttggc
    13681 gatgccgtct gtaagctgga tttcgttgcc cgcgccgcgc ggcagtccgg tcagtaagtc
    13741 gaaaatgcgc ggggtaagga tgtagcgtcc gacgacggca aggttggagg gcgcgtcttc
    13801 gggcttgggt ttttcgacaa tgccggtaat gcgttggaac tgtttgagct gttcggtttc
    13861 gacgatgccg tatgagccgg tttgcgatgc ttcaacggtt tctacgccca aaatgctgtt
    13921 gccgctgcgc ccgtacactt cgaccatttg tttgagcgcg cctttggggg catcaatcag
    13981 gtcgtcggca aggataacgg caaagggttc gtctccgatg gcggcgcggg cgcacaagac
    14041 ggcgtgtccc aagcccagtg cttccgcctg acggatgtag aggcaggtaa tgttcggcgg
    14101 caggatgttg cggacgtgtt ccaacaattt gtctttatgg cgcatttcca actcggtttc
    14161 gagttcgtat gccttgtcga aatggtcttc gatgctgcgt ttgttgcgtc cggtaacaaa
    14221 caccatttcc gtgcagccgg cttccacggc ttcttctacg gcgtattgga tcagcggctt
    14281 gtcgacgatg ggcagcattt ctttcgggct tgccttggtg gcgggcagga agcgtgttcc
    14341 catccctgcg acggggaaaa cggctttccg tatgggtttc attctttttc ctttgtattg
    14401 ttttgatgtt taaagggcga gtttgcgtaa gagttcggca agtgcctgcg cgcggtggct
    14461 ttcgcggttt ttgacctccg aatccaattc ggcggcggtt ttgccgtgtc cgggcagata
    14521 aaaatacggg tcgtaaccga aaccgttttc accggcggga acgccgttcc attgtccgtg
    14581 ccatacgcct tcggcaataa tcggacgcgg gtcgtcttta tggaggacaa aaaccaatac
    14641 gcagacgtag caacagcttt tgtcggcttt gccggcaagt tcggcggcaa gtttcaggtt
    14701 gttggcggta tcggatttgg gatggtcgcc cgcgtaacgt gcggaatgga tgcccggcgc
    14761 gccgtttagg gcggcggcac agatgccgct gtcgtcggcg agtgcgggca gcccgctgta
    14821 tttggcggca tggcgtgctt ttgccagcgc gttttcgaca aaggtggaat agggttcggg
    14881 gcattcgggt atgccgaatg cggattgcgg caatacggtg atgccgtaag gtttgaataa
    14941 gttgccgaat tctttgagct tgccggcatt gccgcttgcc aaaacgattt tttccgtttt
    15001 ttcagacata gcggttttcc tttgcggcgg attgggcggc gcgtagggat ttgtgccgca
    15061 ggtagagggc gagtgcgccg atttgcccga acagcgcgcc gaatgcgaaa aggaaggacg
    15121 cgacggcgaa ggctttgctg ccggcggtca gcagataagc acccaagagg acgagcagca
    15181 taaatgccag tgtgaacagg gcaatagaca acagatagat ttttcggcgg ttcatggcgt
    15241 tcggtcggaa acggtatgtt cggattatat ccgattggga cggtattccc tagaggctgg
    15301 aaaaatgccg tctgaaacgg cgttcagacg gcatggcggg cgttatgcct gtttgttcca
    15361 acgttcgatg gattctttga tgactttttt cgcttcttcc gcatcgcccc acgattcgac
    15421 tttggtcgta cctgcttttt tcaggtcttt gtaatggttg aagtggaact cgatttgttt
    15481 gatgagctgt tgcggcaaat cggacaaagt tttgtaggcg ttgccgttgt tgcggtcgtc
    15541 ggcaggaacg cagacgattt tgtcgtctac ttcgccgtcg tcaacgaatt tcatcacgcc
    15601 gataacgcgc gcttccaaga atacgccggt tgccaaaggt tgttcggtaa cgagcagcac
    15661 gtccaattcg tcgccgtctt cgtccaaagt ttggggaatg aagccgtagt tggtcggttt
    15721 ggcgaagatg gcgggttcga cgcggtcgag ttggaatgcg gcgagtttgc ggttccactc
    15781 gattttgtgg ttgctgccgg cggggatttc gttgacgacg ttgatgatgc cgccgtccac
    15841 atcgccgggg gtcaggattt ggttgaagtc tgccatttgg tttcctttgt ttgggaaagt
    15901 ttgaagtttg aaagtatagc acaaacgtcc ggctgaaaat gcgcccgatg ccgtctgaaa
    15961 gggtgtacgg gcgcgtgtta ccgtttgccc aaaaacctgc ccagttccag aatcgcgcgc
    16021 ctgttggacg gggaaaacac tttttccgcc gcttcttcca agccgaacca gccgtaggag
    16081 acgtgttcct cgggttgcag ggcgatgggc gtatcgcgcg ggatttcggc agagaagagg
    16141 tgttcgcggt tttcaaacac gccttttgga tagcggtgcc gccagtggtg gtagatttcg
    16201 taaaccgtgc tgtcgtgcca gtcttgaagc tgtccttccg ccagcagtat gccggtttct
    16261 tcccaaactt cgcgccttgc cgtttgggcg acggtttcgc ccggttcgag gctgccggtt
    16321 accgactgcc aaaatccttt cggatgcgtg cgttcgatga gcaggatgcc gccgtccccg
    16381 ctataaagga cgaccagtgc ggagacgggg tatttgagcg gttttgccat cggcatcttt
    16441 cggcgggctg cggtaatgaa ggggcggatt atagcaaacg ccgcacgtta tggcgtttat
    16501 ccttttccgt atcctttttt ctgcacggat gggacgcgcc ggtgtttgcc ggtaaatttt
    16561 ccgttgtgtc aaaaagataa gggcggttgt gattttaatg cttgccaaag cgtcgggcgg
    16621 aaactataat ccgaaactta tcgagtcgga gtgtggcgca gtctggtagc gcacttgcat
    16681 ggggtgcaag gggtcgaagg ttcgaatcct ttcactccga ccaaaaattc cgaaagccgc
    16741 tttcaaaagc ggcttttttg ccgtccgtat gattatgatg tagagtacgc ggcgacagac
    16801 attcaaatgc cgtctgaaaa ccgttcagat ggcatctctt tatcttagtt tcattccgta
    16861 ccatcttaag gaacatcaaa ttgggcattt cccgcaaaat atcccttatt ctgtccatac
    16921 tggcagtgtg cctgccgatg catgcacacg cctcagattt ggcaaacgat tcttttatcc
    16981 ggcaggttct cgaccgtcag catttcgaac ccgacgggaa ataccaccta ttcggcagca
    17041 ggggggaact tgccgagcgc agcggccata tcggattggg aaaaatacaa agccatcagt
    17101 tgggcaacct gatgattcaa caggcggcca ttaaaggaaa tatcggctac attgtccgct
    17161 tttccgatca cgggcacgaa gtccattccc ccttcgacaa ccatgcctca cattccgatt
    17221 ctgatgaagc cggtagtccc gttgacggat ttagccttta ccgcatccat tgggacggat
    17281 acgaacacca tcccgctgac ggctatgacg ggccacaggg cggcggctat cccgctccca
    17341 aaggcgcgag ggatatatac agctacgaca taaaaggcgt tgcccaaaat atccgcctca
    17401 acctgaccga caaccgcagc accggacaac ggcttgccga ccgtttccac aatgccggta
    17461 gtatgctgac gcaaggagta ggcgacggat tcaaacgcgc cacccgatac agccccgagc
    17521 tggacagatc gggcaatgcc gccgaagcct tcaacggcac tgcagatatc gttaaaaaca
    17581 tcatcggcgc ggcaggagaa attgtcggcg caggcgatgc cgtgcagggc ataagcgaag
    17641 gctcaaacat tgctgtcatg cacggcttgg gtctgctttc caccgaaaac aagatggcgc
    17701 gcatcaacga tttggcagat atggcgcaac tcaaagacta tgccgcagca gccatccgcg
    17761 attgggcagt ccaaaacccc aatgccgcac aaggcataga agccgtcagc aatatcttta
    17821 tggcagccat ccccatcaaa gggattggag ctgttcgggg aaaatacggc ttgggcggca
    17881 tcacggcaca tcctatcaag cggtcgcaga tgggcgcgat cgcattgccg aaagggaaat
    17941 ccgccgtcag cgacaatttt gccgatgcgg catacgccaa atacccgtcc ccttaccatt
    18001 cccgaaatat ccgttcaaac ttggagcagc gttacggcaa agaaaacatc acctcctcaa
    18061 ccgtgccgcc gtcaaacgga aagaatgtga aactggcaaa caaacgccac ccgaagacca
    18121 aagtgccgtt tgacggtaaa gggtttccga attttgagaa gcacgtgaaa tatgatacga
    18181 agctcgatat tcaagaatta tcggggggcg gtatacctaa ggctaagcct gtgtttgatg
    18241 cgaaaccgag atgggaggtt gataggaagc ttaataaatt gacaactcgt gagcaggtgg
    18301 agaaaaatgt tcaggaaacg agaagaagga gtcagagtag tcagtttaaa gcccatgcgc
    18361 aacgagaatg ggaaaataaa acagggttag attttaatca ttttataggt ggtgatatca
    18421 ataagaaagg cacagtaaca ggagggcata gtctaacccg tggtgatgta cgggtgatac
    18481 aacaaacctc ggcacctgat aaacatgggg tttatcaagc gacagtggaa attaaaaagc
    18541 ctgatggaag ttgggaggtg aaaacgaaaa aaggtgggaa agtgatgacc aagcacacca
    18601 tgttcccaaa agattgggat gaggctagaa ttagggctga agttacttcg gcttgggaaa
    18661 gtagaataat gcttaaggat aataaatggc agggtacaag taaatcgggt attaaaatag
    18721 aaggatttac cgaacctaat agaacagcat atcccattta tgaatagtaa tatttatgaa
    18781 aaattaggag attaatgatg aaaagaatta agtgcttttg tgataaattt ccatcaggag
    18841 atacatttag aatgtgtatc attctggatg actatgataa tagggttgat tattatgtag
    18901 gaatatatga ttacattacg tctaccttaa tgagcgatat ttactatcga tccacgattg
    18961 atgagcattt caagattata gaattaatag aaaataatcc aaatgaaatt tatgatgatg
    19021 gcggtggtca acaattttgc ctagaatttc atcatgataa ggtcattttt tatcacaatg
    19081 aatttgatga agaagatggt tatccagtat taagctgttc gctgcatact tttaaaactg
    19141 ctttaattgc ttggaatgct tttttgcaat tgcctaaaag tattcattcg gtggtggaga
    19201 ctgtgattga ggaataagca taattagctt aatgaataga atcagcgata tagaaaggta
    19261 gaatcagata tatcccacct aaaaattact ctccttcagc accgctacca aaaggaccta
    19321 ataatggata tagtgccggg aatttaagca aaaaagtcct gactttagtt tacataaacg
    19381 gcaaactcaa attcgggcca agcagtctgc cgtcttcggc attgaaagca cctgttccca
    19441 ttgtcggcaa ggctgaggct ccgcccggaa aagctgtgcc tgtcggcgaa gcccgcattc
    19501 cttcaaatcc aaaagcggta aaaggaacaa ccgttaccct gtcgcaggaa caacgcctag
    19561 ccaacggaga gctgataccg aaaggctctg tggtaacgtg gtcggacaat gcggtgaagg
    19621 ttgtgcggcc tgatgggaaa aagcggagat ttaatagtcg ggattgggaa cagaaggcat
    19681 tgccgaaacc gcaactaccg aaagattaca gagatgacat caacataggt ggaaataatc
    19741 ctagatttaa tcagcaacgg catgaaaaaa tcgaatccca caacccaaac agaatagagg
    19801 agatacgcca acgtaatgcc gtttcttctg cagaaaggca ggctgatgca ttaaaaagga
    19861 tgcaaacgtc tggccgcacc cgcctgcctg ggcagccaca aaaaggaaaa cccggcgctg
    19921 ctagagaaat gtcctcttca gacaatccga atcaaacagc cgaggctttt gcacgtcgat
    19981 atttaggcag gaaacctaca ctaatggaac gagtcgaagg tgaaaaatta tacggaggta
    20041 aatgtgtggg ctgttggaaa gcgcaaatgc cggacggaac ctttgtggtg tataggccag
    20101 caggtaatgc ttctatgacc aagggaacta ctgcgtctgt tgatattaat aacccaacat
    20161 taaataactt aaatattaat ttaaaaggca agccaacaga gttaaaattt aaattccctg
    20221 ctagataagg atgttttaat gaatttagag acagcagaaa atattcaatg cgaatttgag
    20281 tatttagcat tagatggtta ttttccaatg cattttgcat cgcatggtca aggaaacaaa
    20341 gattggcaat ttgcagttga attcatatat agattattaa tatgtcaatt agcaactctt
    20401 gaacctatca actttgaaac gaaaaatgat attttagact tttgccataa tttagcaaaa
    20461 caaagtcctt ttaataatga taatgaggtg tggtatcagg gggaaattgt attaacaaaa
    20521 aaagggatag atttaataaa ggaatatatt ccagaggcgt ttgaagagtg gaatggaaag
    20581 aaatttgaat taaacattcc tttcattaaa acattaaaaa atatatttgt taattatgag
    20641 gtagcatggg atgagaataa tccactattc cccattatat ctttaaacct cggcacctga
    20701 taaacatggg gtttatcaag cgacagtgga aattaaaaag cctgatggaa gttgggaaat
    20761 gaaaaaagga aggggcggaa aacctatgac aaatcattct atgttcccaa aagattggga
    20821 tgaggctaga attagggctg aagttacttc ggcttgggag aaaagaaagg aattagaagg
    20881 tcaaaaatgg agaggtacaa gtaaatctgg tgttgaaata gaaggattta cggaacctaa
    20941 tcgaacagct tatccacttt ataatagtaa atgaagtatt gattaaaaag gtgtttttaa
    21001 atgtataaaa taaaatgttt ttatgatcag tttagttctg gtgaattatt taactactgc
    21061 cagtttttag ataattcttc taatcaaaag ataaatacac gaggaacggt gtatcaatat
    21121 attatttatg ttttaacagg agacctttat ctacaaaaag acatagatga aaatttagaa
    21181 tttatccatc aagcagaaaa taatcccaat gaagtttatt caggaggtgg acaaggtttt
    21241 tgctgggata tttctgctga aaaagtagtt ttctatcaca acgaatttga tgaagaagac
    21301 ggctggcctg atttaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat
    21361 gcttttttgc aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa
    21421 gcataattag cttaatgaat agaatcagcg atatagattg gactgcaaat ccacgcttat
    21481 gcgctgtgcc atgattaaca ggaagagagc aacttggatg ggctagtagg gatggtaagc
    21541 atttaaatat atcaattgat ggaaagatta cacacaaatg actaatttaa aattagattt
    21601 ctactctgaa gttattataa aagattcttg ccctaatgat ttgttagaaa atggtgagac
    21661 tattaaagga aaaaaaggag tggtattagg tataagcgaa gaagatggta taatctatgg
    21721 ctatactatt ttactttttg atataaaata ttgtatatat atagataaga aatatattat
    21781 accgactgga aaaaaattct ctcgcgatga tttttattag ctaatttaag taatttaagc
    21841 aagtttttta gatactgaaa ctggtaatta tgtttatgta agcataagga gttcagattg
    21901 aaggattcta cagggcaaat gaggctggca accaaggatt tggcggaagc cattaaacga
    21961 ggagaagtac gtagttctgc ttttacaaca aagcaactaa aggcaatcga aaaaggagaa
    22021 tctaaaatac caaattacac ttggcatcat catcaagaca ctggaaggat gcaattgatt
    22081 aatgaagact tgcatcatga taccggccat atcggttgga gagcgatgag taaaggaaag
    22141 taactatgtg gaaaatcata aaagaggata gtaatgattt aggatttgca attaaatgct
    22201 tattctctca gtctattgat ttaaatgaat tcaaattatg gattgaacaa gtaatacgag
    22261 atatgcccat cgaggacatc ccgttttata tttttgattt ggcggatttt gatgggggaa
    22321 ttgccgatat tgacaatatt gtaggttttg tttcgagtta cagcctatca aaatcgaaaa
    22381 aaaatgcctt gacaggcatt gccttcttaa gggggataga tgtctatgat ccgcctattt
    22441 caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag aggtttcagc
    22501 atttctttcc gtttgtagag ctgcccccgc tttaaacagt caaaataccg tctgaaagcc
    22561 atttccgccg
//
LOCUS       DAWFKN010000025        22247 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFKN010000025 DAWFKN010000000
VERSION     DAWFKN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.025"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGR46_000986"
     CDS             complement(120..1034)
                     /locus_tag="WGR46_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0855944.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGR46_000987"
     CDS             1290..2759
                     /locus_tag="WGR46_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0855945.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGR46_000988"
     CDS             3011..3382
                     /locus_tag="WGR46_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855946.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGR46_000989"
     CDS             3455..4864
                     /locus_tag="WGR46_000989"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0855947.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGR46_000990"
     CDS             4910..5704
                     /locus_tag="WGR46_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855948.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WGR46_000991"
     CDS             5892..6668
                     /locus_tag="WGR46_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0855949.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHSRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7326..9797)
                     /locus_tag="WGR46_000992"
     CDS             complement(7326..9797)
                     /locus_tag="WGR46_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0855950.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDREEAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGR46_000993"
     CDS             complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGR46_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0855951.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KIDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(10977..12257)
                     /locus_tag="WGR46_000994"
     CDS             complement(10977..12257)
                     /locus_tag="WGR46_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0855952.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12410..13129)
                     /locus_tag="WGR46_000995"
     CDS             complement(12410..13129)
                     /locus_tag="WGR46_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0855953.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGR46_000996"
     CDS             complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGR46_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0855954.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSRSVCSF
                     RQLGLMP"
     gene            complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGR46_000997"
     CDS             complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGR46_000997"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0855955.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15609..17018
                     /gene="leuC"
                     /locus_tag="WGR46_000998"
     CDS             15609..17018
                     /gene="leuC"
                     /locus_tag="WGR46_000998"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0855956.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17115..17369
                     /locus_tag="WGR46_000999"
     CDS             17115..17369
                     /locus_tag="WGR46_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855957.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17431..18072
                     /gene="leuD"
                     /locus_tag="WGR46_001000"
     CDS             17431..18072
                     /gene="leuD"
                     /locus_tag="WGR46_001000"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0855958.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18241..19512
                     /locus_tag="WGR46_001001"
     CDS             18241..19512
                     /locus_tag="WGR46_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0855959.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19528..20259
                     /locus_tag="WGR46_001002"
     CDS             19528..20259
                     /locus_tag="WGR46_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0855960.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20293..21363
                     /gene="leuB"
                     /locus_tag="WGR46_001003"
     CDS             20293..21363
                     /gene="leuB"
                     /locus_tag="WGR46_001003"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0855961.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21526..22089
                     /locus_tag="WGR46_001004"
     CDS             21526..22089
                     /locus_tag="WGR46_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0855962.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5349 a         5864 c         5783 g         5251 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcggggacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc actccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac agaaggcagc
     4981 catcgccccc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gtccccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtcc ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acggcagttc ctcagtcagg cgtttgcgcg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcagatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctagt tgccggcgac ctgaatccag gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacagccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 taaccgtttc tccgaaaaca ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gaatgacgac ggtgttttgc cgtagccaaa
     6901 gcagaaaaac atccctattg tcatttcatc atgatatgac aataggatgt taaaaattaa
     6961 ttatcattat atgattgata tataagaaaa actgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc atacacagaa
     7081 ccggtatttg gaaaaaaaat aatccatatt gacatcatta ttccatttat cgcataaatc
     7141 cactttttaa ttttttttcc tcattaatat aatctcttat ttaataaaat caaataccta
     7201 gcaattcgat ctcaaatttt aaacgccaac gtgtttgagc ggtaacaatg catactttaa
     7261 gtttgtaatc cttcatcgtc tcatttttgt tttaatcgac tatacgtcta aagcaacgcc
     7321 gtccgtcatt gttcggacgg cattttttca gacggcattt tcccccgttt gtgaaggcgc
     7381 agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc tatccacggt
     7441 tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc cgcccatatc
     7501 aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg ggcgacggta
     7561 cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc cgtcccgccc
     7621 gctgcggcgg cggatacgtc tgcctgagcc aaaacgggcg cgtcgttgat gccgtcgcct
     7681 atcatcagca ctttttttcc ttctttttgt aaggctttga cgtattccag tttgtcctcg
     7741 ggcatggctt gggcgcggta gtgcgcgaca cccagggcgc gtgcggtttc ggcaactgct
     7801 tcttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa ctgccacacc
     7861 gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg gaaaccgctt
     7921 tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt acctgaaatt
     7981 tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt gacggtcagt
     8041 tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcaat gtcggggacg
     8101 ctgccgcctg aaatgcggca gttgaggatg gcgcgcgcga gcgggtgttc ggactgctgt
     8161 tccaaacttt gcgccactgc cagcacttgg gcttcggtca tgctgccgag cagttcaatc
     8221 cggcggacgg cgggattgcc ttgggtcagc gtgccggttt tgtcgaaaat gatgtcggtg
     8281 gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc gcgcgccagc
     8341 gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca ggggcaggta
     8401 atgaccagca gggcgacggt aatccacaat gcggtgtgcg cgtcggcgta cagcgtccag
     8461 ccgatgaaga ctgggacggc aagcaggagt tcgccgaata tgaaagacga ggcgtattgt
     8521 tccgccaact cggcagtgcg cggtttttgc gctaaggcgc ggtcgagcag gcggacgatg
     8581 tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg gctttgcgtg
     8641 ttgagtgtac ctgcggttac tttttcagac ggcattttgg cgacgggcag gctctcgccg
     8701 gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc aacggggatg
     8761 gtttcgcccg gtttgaccag caccacatcg cctgcttgga gcttgacgac ggcggcttcg
     8821 cggacatcct gtacggcagg atagccgggc atccggtggc agaaggcggg tatcagtttc
     8881 accagccttt cggcagcatc gcctgccttg cgtcgggcaa tgtgttccat aaagcgtccg
     8941 cccagcagga aaaacagcag catggctatg gattcgaaat acatcccctg ccccgcattg
     9001 gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc aatcggcgta
     9061 tccatgccgg cgcggcggtt tttcaggtcg cgcaacgcgc cttgataaaa cgggacggcg
     9121 caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat ttgcaggaaa
     9181 tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat cgtctgcatc
     9241 atccccagcc cggcaacggc gaggcggacg atgtattgtt ttcgttcttt ttggttggcg
     9301 gcttcgattt tttgcgcgtc atagggtgcg gcggtgtatc ctatctgcct gattttcaac
     9361 agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg gtgcgtgctg
     9421 taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc gatcagccag
     9481 acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt gccgccgtgg
     9541 gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg gtcgaggatt
     9601 tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta ataactgccc
     9661 aagcccgcgt caataatgct ttgtgcgacc gcctgacagc cggcgcagca ggtttctcgg
     9721 tcttcgcctt cgtagcggac ggtcagatgc aggttttcgg gaacatccag accgcagtgg
     9781 aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggtg gcacaatact
     9841 cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa atgcggacgg
     9901 cggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca gacggcagca
     9961 ggtcgatttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt tcccgcagta
    10021 cgttttccac gtcttcaatc tgctgtccgc tgttcggggc ggcggcatcg acgacgtgca
    10081 gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaatgccgaa atcagtttgt
    10141 gcggcaaatc actgacgaat ccgacggtat cggtcaggat aatgctgcat tcgggactga
    10201 tgtacagccg ccgcgccgtc gtgtcgagtg tggcgaaaag ctggtctttc gcatatatgc
    10261 ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag ccgaccagcg
    10321 caaacgtttt gattgtgccc gattcgcggg acttgcggcg cagggcgcgc tgttttttga
    10381 ggttggcaag ctgttttttc aaggcattga tccgatgggc gatcaatcgg cggtcggttt
    10441 ccagtttggt ttcgccgggg cctttcatgc cgataccgcc gcgctggctc tgcagatggc
    10501 cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg acttgcagcc
    10561 tgccttcctg cgtgcgggcg cggcgggcga aaatcgccag aatcagccct accctgtcca
    10621 atacgcggca tttcagttct ttttcaaggt tgcgttcctg cgtgggcgtg agttcgtggt
    10681 tgaatacgac caaatcgatg ccgtctgcgg caactgcttc tgacagctcc gccgccttgc
    10741 ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc acgcgcacgg
    10801 aatcgccgcc cgccgctttg accagctcga cggcttccgc caatgccgtc tgaaaaccgt
    10861 tcagacgggc ggcactgctg cccgtaccat ctttgtccaa cattacgccc accagcataa
    10921 cgcgttcggg ttgcgcttgg gtactgccgt tgccgcctgt tctgcctgtc atgtattcaa
    10981 tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag cgtgatgcgg
    11041 ctgcccgcca ttccgtcaac gctgaagacg atgccgcccg cctcgtgcct tcctatggac
    11101 aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa acaccagcgt
    11161 ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagcgtctc gccgccgttt
    11221 acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc ctgcgcgcgc
    11281 cgtccgttgc ggacatagtc gaataccgcc gtataaagcg tgccgtattc caaaggcttg
    11341 agcgggaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt gccggcggtt
    11401 aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact tttcatcgta
    11461 attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat ttcatattcc
    11521 ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgctgcc gacgggatag
    11581 gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac cgcaccgtta
    11641 tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc ttgggcgcaa
    11701 tgccgctcaa acctgccgtt gccctgattg aatacgagaa cggttttacc gttttcgcgc
    11761 acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg gtggtagatt
    11821 gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc ggacgattcg
    11881 gcggcttcct cttccgtgct gatgttttca tgcacgccgt tgtagagata cccggcaagg
    11941 cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg gtgttctccg
    12001 tgtccgtctt cgggattgag cgtgagatag cgtgcgtgcc tgcgggcgga gttttccaaa
    12061 accggcgcgt gtgccagctt gtgcaaaccg atttgggctc ggatgcggtt gaggtagtgc
    12121 agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc gcccgcgggc
    12181 agggattggt tttgggtatg gtagaaaacg cctgccgccg aacccaacag gagcagccga
    12241 ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt ttatcaaaac
    12301 ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac ctttccccgt
    12361 gccgatgccg aacccgatgc cgtctgaacc ttcagacggc atcgggtgtt tatttgtcca
    12421 cccggatggg ggcaggttcg tattgtgtcg attcgtcgcc gtaatacagc acgccgagtt
    12481 tgatgggaat gcgtccctgc gatttgcggt gggcattgga atcgcgcaag gaataggcgc
    12541 aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc atgcgcgcgc
    12601 tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa ggcgcggcgg
    12661 cacggcggcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg cccagcgaac
    12721 tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca aaacgcatat
    12781 cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct ttggctttgg
    12841 caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa ggaatgccga
    12901 acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg tgcggatgga
    12961 tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc gcctccatca
    13021 cttcgccgct gcacggggcg caacaggaat gcagcaagac ttttttatgt ccgccgggcg
    13081 ggacgagtat ggggcggtcg atgtcggtaa cgatgcgtgt tgtgtccata gtttcaaata
    13141 gatgttctgt ttggtgcaaa cggctatttt atcagagccg ccgcatgtga aacagagtgt
    13201 cagggcatca acccgagctg cctgaacgag cagacgctcc gggaggttac gataaaatgg
    13261 tcgagcagcg acacatcgac cagcgacatg gcctgtgcca gccgccttgt gaacatgatg
    13321 tcttcctgcg aaggttcggg cgagccgccc ggatggttgt gcgcaataat caggctgtcg
    13381 gcatattcgt ccaatgccag tttgacgatt tcgcggatgt aaatcgtgtt ttccgcaacc
    13441 gtaccgcgcg acagctctct gaccgcaatc agttggtttt ggcggttcag cagcagcgcg
    13501 acgctgactt cgactttttc ctgccccaaa tgaaagcgta aataatcggc aacggtatcc
    13561 ggatcggaca ggacgatgct ctcctgcaat tcttccgcca atatccgccg cccgatttcc
    13621 ctgactacgg caaactgtgt gaaacttgcc gtccccatcc ctttgtatgc cgacagtttg
    13681 ccgacctccg cgctcatcag cctccccaaa ctgccgaact cctgcagcaa ataacgcgcc
    13741 aaatcgaccg cactcattcc gcgcgtgccg acgcgcagca ggattgccaa aagttcggca
    13801 tcactcaaag ccgccgcccc gcgttccaac agcttttccc tgggtctttc gccttccggc
    13861 cattgcttga tgctcatgtt ttcccttttt taaatagtct gagatgccgt ctgaaacaaa
    13921 tgttcccaca cgccaaatat ccgaaatgcc gtctgaaccg gatttccgct tcagacggca
    13981 tctgtatttc aaaacagtct tacgccgttt cttccaactc gattgaagcc gccagaagcg
    14041 acaggcgtgc cattacgccg taaacgtaga ggcggttgtg ttcgttgtcc acgtcgcagt
    14101 ctttttcaga gcgcggcata cccagcgagt cccattgttc gaatacgcgc ttgcaggctt
    14161 cgggcgcttc ttgggagttg tcgccgttgc cggtggggat gctgttggac agcggaacaa
    14221 acaccatacc gccggcgttg aggttttcgt ccgcaccgcg cccttcgtgt acgcggaaaa
    14281 agccgccgat gacgaagcgg tccatcatat acacgacggg ttcgcacacc gcgccgttta
    14341 aggtttcata agtataaatc ccttcttgga caatcacttc gctgacttcc aaaccttctt
    14401 tgactttcgc cattttattg cggtttttac ggttcaatcc gcgcacttcg tcggcggatt
    14461 tgacgctcat cacgcccatg ccgtaagtgc cggcatcggc tttgacgatg acgaaaggtt
    14521 tgtcggtaat gcccgattcg tcgtatttgg cttgaatttt cgccagcaca cgttctaccg
    14581 cttccgccaa cgcgtcttcg ccttcacgcc cttggaagtc caaaccgccg attttttcaa
    14641 aataagggtt aatttgccat tcgtcgatgc cgattaactt ggcaaattcg gcggtaactt
    14701 ggttgtacgc gccgaaatga tttgttttgc ggcgcgtcgt ccaaccgccg tgcaacggcg
    14761 gcaaaacggt ttggctgatg cctttgagga tgtcgggaat gccggcagac aaatcgttgt
    14821 tcaacaaaac cacgcaaggc gaaaagccgt ctgcaagatg gacgcggtcg cgggtacgca
    14881 ataaaggttc caacaggatt ttgtcgccca atgcagtttc aaattcggtc ggttcggtta
    14941 cttccggatt caggctgccc aagcgcactt cataccctgc cgaacgcaaa atctcgccga
    15001 gggcgtaaat gttttgcagg taaaacgtat tgcgcgtgtg gttttcggga ataatcaata
    15061 cggatttcgc cgtttcgcag gcgcgttgca ccgcatcttg cgcggcaacc gccgccagcg
    15121 ggataaagtt gggattcaga ttattgaagc cgccggggaa caaattcata tcgatagacg
    15181 aaattttgta accggcattg cgtatatcga ccgaaccgta aaacggcggg cggtgtacgc
    15241 tccactgcgt gcggaaccac gcctcgattt tggcgtgatt ggacaggatt tttgcctcaa
    15301 acgcctgaag ttgcgccgaa tgttcgggcg acatcaccgg taatttcatc tttcgtctgc
    15361 ctctgtgtgt tttttgacaa gatgcagatg ataatgcctt tgaaagaatg acgacaagta
    15421 ttctttcgta tcgaatcatg ctttagatat aactgtttac tgaaatggac aatacgtcta
    15481 attttttcgt taaaattgtt tgcaaaataa caccggctca aaaattagac aaaatttgtt
    15541 gcgcggtata aagaatacgt ctaaaatccg cccaatccgc attaccattt atccaaaaga
    15601 acaacatcat gacagcacaa accctctacg acaaactttg gaacagccac gtcgtccgcg
    15661 aagaagaaga cggcaccgtc ctgctctaca tcgaccgcca tttggtgcac gaagttacca
    15721 gccctcaggc atttgaaggc ttgaaaatgg cggggcgcaa gctgtggcgc atcgacagcg
    15781 tcgtctccac cgccgaccac aacaccccga ccggcgattg ggacaaaggc atccaagacc
    15841 cgatttccaa gctgcaagtc gatactttgg acaaaaacat taaagagttt ggcgcactcg
    15901 cctattttcc gtttatggac aaaggtcagg gcatcgtaca cgttatgggc cccgaacaag
    15961 gcgcgaccct gcccggtatg accgtcgtct gcggcgactc gcacacttcc acccacggcg
    16021 cattcggcgc actggcgcac ggcatcggca cttccgaagt cgagcacacg atggcgaccc
    16081 agtgcattac cgcgaaaaaa tccaaatcca tgctgattgc cgttgacggc aaattaaaag
    16141 cgggcgttac cgccaaagac gtggcacttt acatcatcgg gcaaatcggc acggcgggcg
    16201 gtacaggcta cgccgtcgag tttggcggcg aagccatccg cagcctttct atggaaggcc
    16261 gcatgacttt atgcaatatg gcgatcgaag ccggtgcgcg ctcaggcatg gttgccgtcg
    16321 accaaaccac catcgactat gtaaaagata aacccttcgc acccgaaggc gaagcgtggg
    16381 acaaagccgt cgagtactgg cgcacgctgg tgtctgacga aggtgcggta ttcgacaaag
    16441 aataccgttt caacgccgaa gacatcgaac cgcaagtcac ttggggtacc tcgcctgaaa
    16501 tggttttaga catcagcagc aaagtgccga atcctgccga agaaaccgat ccggtcaaac
    16561 gcagcggtat ggaacgcgcc cttgaataca tgggcttgga agccggtacg ccattaaacg
    16621 aaatccccgt cgacatcgta ttcatcggct cttgcaccaa cagccgcatc gaagacttgc
    16681 gcgaagccgc cgccatcgcc aaagaccgca aaaaagccgc caacgtacag cgcgtgttaa
    16741 tcgtccccgg ctccggtttg gttaaagaac aagccgaaaa agaaggcttg gacaaaattt
    16801 tcattgaagc cggttttgaa tggcgcgaac cgggctgttc aatgtgtctc gccatgaacg
    16861 ccgaccgcct cgcaccgggg caacgctgcg cctccacctc caaccgcaat tttgaaggcc
    16921 gtcaaggcaa cggcggacgt acccacctcg tcagccctgc catggcagcc gcagccgcag
    16981 tgaccggtcg ctttaccgac atccgcatga tggcgtaagt attttcaggc ggtctttaag
    17041 aatttgacgc ggccgtctga aatatgtccc gtaaggcaga aacaccttta atattcaaac
    17101 caaggagttt aaccatgaac aaacttttca ttaccgccct gtccgccctc gccttgtccg
    17161 cctgcgccgg cacttgggag ggcgcgaaac aagacaccgc ccgcaacctt gacaaaacac
    17221 aggccgccgc cgaacgcgcc gccgaacaaa caggcaacgc cgtcgaaaaa ggctgggaca
    17281 aaaccaaaga agccgtcaaa aaaggcggca atgccgtcgg acgcggcatt tcccatctcg
    17341 gcggaaaaat cgaaaacgcc accgaataac cgattcgcga tgccgtctga aaccgcttca
    17401 gacggcatcg tcagcacaag acgcaaaccc atgaaagcct ttaccaaaat taccgccatc
    17461 gtcgccccgc tcgaccgcag caacgtcgat accgacgcca tcatccccaa acaattttta
    17521 aaatccatca aacgcagcgg cttcggcccc aatgcctttg acgaatggcg ttacctcgac
    17581 cacggcgaac cgggcatgga caacagcaaa cgcccgttga accctgattt ctcgctgaac
    17641 cagccgcgtt accaaggcgc gcaaatcctg ttgacgcgta aaaacttcgg ttgcggctct
    17701 tcacgcgaac acgccccttg ggcattggac gactacggct tccgcgccgt catcgccccc
    17761 agcttcgccg acatcttctt taacaactgc tacaaaaacg gccttttacc tatcgtgttg
    17821 accgaagaac aagtcgatcg gcttttcaaa gaagttgaag ccaacgaagg ctatcagctc
    17881 tccatcgacc ttgccgaaca aaccctgacc acaccaagcg gcgaaacatt cacattcgac
    17941 attaccgaac accgcaaaca ctgcctctta aacggcttgg atgaaatcgg tctgaccctg
    18001 caacacgccg acgaaattca cgccttcgaa gaaaaacgcc gccaaagcca gccttggctg
    18061 tttaacggtt aagcctatgc cgtctgaaga ccgttcagac ggcattcctg ataacatata
    18121 attgctgcaa ctaatgtaaa cagaggggct ataaattgct ttcatctttc aagcgggctt
    18181 agcaacgctc taaattaatg caatacagct ctatttcaaa taaattttta gatttttaat
    18241 atgcaacaga ttaaatttat tgacttattt tccgggatga gcggtatcag gaaaggattt
    18301 gaacaagcct gtcggaaaca gtcggttgct tgcgaatgtg tttttacctc agaaatcaaa
    18361 ccggcagctt tggaagtatt gaagcaaaac taccctgatg aagtgccgta tggagatata
    18421 acgaaaattg aaacgggaga tattcccgat tttgacatct tgttggcagg cttcccttgc
    18481 caggcttttt ctttcgccgg aaaaagattg ggctttgaag atacgcgggg aacgcttttc
    18541 tttgatgtgg caaggatttt aaaggcaaaa aaaccaaaag gttttatttt ggaaaatgtg
    18601 gaaggattgg tgacgcacga tagaaaagat ccgacacaaa aaataggacg caccctgacc
    18661 gttattttgg aaaccttgga aacattgggc tactatgttt cttggaaagt tttaaatgca
    18721 aaagatttcg gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc
    18781 aaacccgact tgtcttttga aacaacccta agtccgaaat taaaaaatat tttggaatcg
    18841 ggactgccta ccgaaagcag tcctttcatc aaaaaattgc taaagaaatt tcccccgtcc
    18901 gaactgtacg gaaaatcagt aaaggacaaa cggggaggga aaaacaatat ccacagttgg
    18961 gatattgaat taaaaggcac agtaaccgaa gaagagaagc aattattaaa tatgcttcta
    19021 aaagaacgga ggaagaaaaa atgggcttca gaaatcggca tcgattggat ggatgggatg
    19081 cctttgacaa aagcgcaaat ttcaactttc tataaacacc ccaatcttca aaatattttg
    19141 gacagcctga cagataaagg ctatttggtt ttggagcatc cgaaacaaaa aattggcggg
    19201 cgaagaatca aagatgaatc cctgcccaaa ggctacaata ttgtttcagg aaaaaaatca
    19261 tttgaaatca ataaaatatt agacccaaat gatgttgcgc caaccttagt tgcaatggat
    19321 atggagcacc ttttcgtcgt tgacaacggc ggtttgcgca cacttaccgg aaaagaaggt
    19381 ttacgcttat tcggctatcc ggacgattat tcgtttgata ttcctaaaaa agacaaatac
    19441 gatttattgg gcaataccgt tgccgttcct gtgattaagg cggtatctga aaggcttctg
    19501 catactttat aaaccgatag ggataaaatg gtaaaactta ctgcacaaca aatatttgat
    19561 aagttgctga atgaggaaaa aatcttatcg gtcaatggtc aaattagatt tttcttgggc
    19621 gatgtggata ttatcgtcaa acaaaaagat gtcgtcggca acatcattca agaatggctc
    19681 ggcggatggt tgagaaaaag ggaaattgaa tttgatgttt caaccaatac ccaaatgccc
    19741 cccgatttct ttttaaataa aaaagaccgc agtagagaat tgttggaagt aaaggcgttc
    19801 aaccgaaatg ccggcccggg ttttgatatt gcagatttta aaatgtattc tgatgaaatc
    19861 attcataaac cctatatgct ggatgtagat tatttaatat tcggttacga tatggacgac
    19921 aacggcaatg taaccatcaa ggatttatgg cttaaaaaag tatggcaaat tacgagaagc
    19981 atggatgggt gggcaattaa tcttcaagtc aaaaaaggcg tggtgcataa aatccgcccg
    20041 ggtgtttggt acagcataaa taaaaagaat atgcctatgt ttgaatgctt ggaagatttt
    20101 gtttccgcaa ttgaagaaac cgtttatcaa aacccggcaa cacggcataa cgcatcctta
    20161 tggaaaaaga aatttgagga agcctataaa aaacactaca accgatcgat ttccatacct
    20221 cgttggcacg aaattgcaca caaatacaaa aagaaataac ccttcaacca tcaacccata
    20281 aggaaacttg aaatgaccaa acatatcgcc atcctccgag gtgacggcat cggtcccgaa
    20341 atcgtcgccg aaaccgtccg cgtactcgac aaactcatcg cccaaggctt ggacatcagc
    20401 tacgaatacg ccccattggg tggtgaagcc tacgacgaat acggccatcc ttatcccgaa
    20461 tttacgcaaa acctctgccg caaagccgat gccgtcctgc ttggtgcagt cggttctcct
    20521 caatacgaca acctcgaccg tccgttgcgc cctgagcgcg gcctgttggc aatccgtaag
    20581 gatttgaacc tgtttgccaa tttgcgtcct gctgttttat atcccgaatt agccaacgcc
    20641 tccacgctga agcctgaaat cgttgccggt ttggatattt tgattgtgcg cgaactcacc
    20701 ggcgacatct atttcggcga accgcgcggc atccgcgttc tggaaaacgg cgaacgcgaa
    20761 ggctacaaca ccatgaaata cagcgaaagc gaaatccgcc gtattgccca tgttgccttc
    20821 caatccgccc aaaaacgcag caaaaaagtc tgttccgtag gcaaagccaa cgttttggaa
    20881 accaccgaac tgtggcgcga aatctttgaa gaaatcggca aacaatatcc cgatgtcgaa
    20941 ctctcccata tgtatgtgga caacgccgct atgcagctcg tgcgcgcgcc caaacaattt
    21001 gacgtgattg ccaccggcaa catcttcggc gatattttat cggacgaggc ctctatgctg
    21061 accggctcca tcggtatgct gccttctgct tctttggacg aaaacggcaa aggcctgtac
    21121 gaaccgtctc acggctccgc tcccgacatc gccggacaaa acaaagccaa cccgctggcc
    21181 accatcctct cgctcgcaat gttgctgcgt tacagcctga acgacgaagc gcgcgcgcaa
    21241 caagttgaaa acgccgtcca aaaagtgctg caacaaggct tgcgtaccag cgacatttac
    21301 gaagaaggca caaaactcgt ttcctgctcc gaaatgggcg atgcgatact cgccgccttg
    21361 taaaaaaaca aagcgcggat cacaccgcgc tttttcatct gcccttttcc gcccgcaccg
    21421 ttcgtcaacc gcctgcaaac ccaccgttta ccggcaggaa atgccataaa tccctgccta
    21481 tgttaggata aacgccttta ccggcccaca caaaggatta tcatcatgaa aaaaatcatc
    21541 ttcgccgcac tcgcagccgc cgccatcagt actgcctccg ccgccaccta caaagtggac
    21601 gaatatcacg ccaacgcccg tttcgccatc gaccatttca acaccagcac caacgtcggc
    21661 ggtttttacg gtctgaccgg ttccgtcgag ttcgaccaag caaaacgcga cggtaaaatc
    21721 gacatcacca tccccattgc caacctgcaa agcggttcgc aacactttac cgaccacctg
    21781 aaatcagccg acatcttcga tgccgcccaa tatccggaca tccgctttgt ttccaccaaa
    21841 ttcaacttca acggcaaaaa actggtttcc gttgacggca acctgaccat gcacggcaaa
    21901 accgcccccg tcaaactcaa agccgaaaaa ttcaactgct accaaagccc gatgttgaaa
    21961 accgaagttt gcggcggcga cttcagcacc accatcgacc gcaccaaatg gggcatggac
    22021 tacctcgtta acgttggtat gaccaaaagc gtccgcatcg acatccaaat cgaggcagcc
    22081 aaacaataaa tcttgatgcg aatcagcaca aatgccgtct gaacgtcctt caaacggcat
    22141 ttgtgccgtc ttcatcaaac cgccccgcca acggtaagcc gatatggcgg agcacaatcc
    22201 gccgaaagat atttcagcgg caggcagggc ttgccggaaa gtccgga
//
LOCUS       DAWFKN010000026        21718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFKN010000026 DAWFKN010000000
VERSION     DAWFKN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.026"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            42..593
                     /locus_tag="WGR46_001005"
     CDS             42..593
                     /locus_tag="WGR46_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855963.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWAHALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(870..1562)
                     /locus_tag="WGR46_001006"
     CDS             complement(870..1562)
                     /locus_tag="WGR46_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0855964.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1868..2425)
                     /locus_tag="WGR46_001007"
     CDS             complement(1868..2425)
                     /locus_tag="WGR46_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855965.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(2669..2827)
                     /locus_tag="WGR46_001008"
     CDS             complement(2669..2827)
                     /locus_tag="WGR46_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855966.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2824..3666)
                     /locus_tag="WGR46_001009"
     CDS             complement(2824..3666)
                     /locus_tag="WGR46_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0855967.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDSIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLTQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3732..3875
                     /locus_tag="WGR46_001010"
     CDS             3732..3875
                     /locus_tag="WGR46_001010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855968.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            3905..4825
                     /gene="pyrB"
                     /locus_tag="WGR46_001011"
     CDS             3905..4825
                     /gene="pyrB"
                     /locus_tag="WGR46_001011"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0855969.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4835..5293
                     /locus_tag="WGR46_001012"
     CDS             4835..5293
                     /locus_tag="WGR46_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0855970.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5358..6023
                     /locus_tag="WGR46_001013"
     CDS             5358..6023
                     /locus_tag="WGR46_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855971.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6086..7303)
                     /locus_tag="WGR46_001014"
     CDS             complement(6086..7303)
                     /locus_tag="WGR46_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0855972.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7445..7990
                     /gene="def"
                     /locus_tag="WGR46_001015"
     CDS             7445..7990
                     /gene="def"
                     /locus_tag="WGR46_001015"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0855973.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8077..9003
                     /locus_tag="WGR46_001016"
     CDS             8077..9003
                     /locus_tag="WGR46_001016"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0855974.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9082..10341
                     /gene="rsmB"
                     /locus_tag="WGR46_001017"
     CDS             9082..10341
                     /gene="rsmB"
                     /locus_tag="WGR46_001017"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0855975.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10307..10906
                     /locus_tag="WGR46_001018"
     CDS             10307..10906
                     /locus_tag="WGR46_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0855976.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10906..13023
                     /locus_tag="WGR46_001019"
     CDS             10906..13023
                     /locus_tag="WGR46_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0855977.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            13016..14293
                     /locus_tag="WGR46_001020"
     CDS             13016..14293
                     /locus_tag="WGR46_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0855978.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14384..15571
                     /gene="dprA"
                     /locus_tag="WGR46_001021"
     CDS             14384..15571
                     /gene="dprA"
                     /locus_tag="WGR46_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0855979.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15597..16058
                     /locus_tag="WGR46_001022"
     CDS             15597..16058
                     /locus_tag="WGR46_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0855980.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16130..18436
                     /gene="topA"
                     /locus_tag="WGR46_001023"
     CDS             16130..18436
                     /gene="topA"
                     /locus_tag="WGR46_001023"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0855981.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPSRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18509..19204)
                     /locus_tag="WGR46_001024"
     CDS             complement(18509..19204)
                     /locus_tag="WGR46_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855982.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(19224..19775)
                     /locus_tag="WGR46_001025"
     CDS             complement(19224..19775)
                     /locus_tag="WGR46_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855983.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19894..20272
                     /locus_tag="WGR46_001026"
                     /pseudo
     CDS             19894..20272
                     /locus_tag="WGR46_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            20361..20930
                     /gene="rsmD"
                     /locus_tag="WGR46_001027"
     CDS             20361..20930
                     /gene="rsmD"
                     /locus_tag="WGR46_001027"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0855984.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21053..21304
                     /locus_tag="WGR46_001028"
     CDS             21053..21304
                     /locus_tag="WGR46_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0855985.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            21401..21484
                     /locus_tag="WGR46_001029"
     tRNA            21401..21484
                     /locus_tag="WGR46_001029"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21435..21437,aa:Tyr,seq:gta)
     gene            21515..21588
                     /locus_tag="WGR46_001030"
     tRNA            21515..21588
                     /locus_tag="WGR46_001030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21547..21549,aa:Gly,seq:tcc)
     gene            21598..21672
                     /locus_tag="WGR46_001031"
     tRNA            21598..21672
                     /locus_tag="WGR46_001031"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21630..21632,aa:Thr,seq:ggt)
BASE COUNT         5371 a         5444 c         5986 g         4917 t
ORIGIN      
        1 gccccttgtt ttttgttcgg cggcttgcgt ggtcgggtaa aatgaaagtt ttgaacggtt
       61 ggtcggacag gaagatgtgg cgggttttga gtgctttgcc gataggcgtg gtgttttttg
      121 atttgatcta cggttttgtg ttgaatgtgt tgcagggttt ggatttgcag cgtgccgtgc
      181 cggattcgga aggcgtgttg gcggttacgc ccgatattgc attcaacagt ttgcagattg
      241 tcgccaacgg cggtatggcg gcggtggtct gtttcgggtt ggcggttgtg tttttgctca
      301 accgttcggt gcggcggcgg caggtgttgg aaatcggggt gttccggatg ttggggctgg
      361 tggcggtatt ggcgttcagc gcgccgtcgg tgtgggagtg ggcgcacgcg ctgccgctgc
      421 tgctgaaggg cgcggacgtg gtcaatacgg ggaatgcgcg ttatgtgctg acggctttgt
      481 gtatgccctt tccggcggtg tcgtgcgtca tcgggctggt ggggcggttc aggcttcaga
      541 cggcatcggg cagggcggca aagtcagggg gtgcgggcaa ggcggacgga taggacgcat
      601 ttttcagcgg gtgcgtcgag aagcagccga tgtgtttggc agccgcagct tgggggggtg
      661 tagtgctaat ggcggtttct ttgcttttat agtggattaa caaaaaccag tacggcgttg
      721 cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt
      781 ccgtactatt ggtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
      841 tataaaataa atgggcaaaa atcggtttat tatcgttttt gccgcatttg gatttgttct
      901 accgtaaaac gtgtttgacg aacgggattc ttattaaaaa acatctgatt tctaacaaaa
      961 tcagtatttt ttggcacgat ggctaaaatt tttccttcca tttcgccatc acgtgttttc
     1021 catgcgctca agaattgtga tttgctcatt gagacgtgcc ccagcgatgg atcagccagc
     1081 aaaacagttt ctccgttaat accgttcaat accgaaaaat ggttgttttt acggtatttt
     1141 aaatacacaa ttacaggaat ttttagttgt accaactgtt caaatggcaa agcataacct
     1201 tgtgcttcaa aacccagttc gggcattatg cgttgcatat catcaaaaga agcacgcatt
     1261 tgggttttat ccattttgtc caagatttcc gcttcagaat aatgtctgcc ataaaaatta
     1321 ttcagtaacg tggcaatcga agccgcgccg caagaaaaat ccaaatcttg ttttactatg
     1381 ccggaatctc gccgtgcttt ccaactccgt acatggatgt tttggtaaga agcggggttt
     1441 tcagcaaata tagggtaagc aaaaacaata tttgggataa agccaattaa aaccgcatgg
     1501 tttattaatt tagaaatatt ttttatcata gtatatatgc gtagaataaa cgcatgaaac
     1561 ataaaaaaat aaaatcatat gcaattttat tgcatgaata aatatgaata aaatagataa
     1621 tgatgggagt aaatacgcca tgtattttgg aagtttaaat ttattaataa taaaataatt
     1681 tatcgtagcg caaataaatc ccaaaattgg aacgattaga aaaaaaatta atacacccat
     1741 catcatgcaa actctatatt aataaatagc taactaattc tattgtggaa attagttagc
     1801 taactaaaag ttattaatga ttatttccga gaattgactg cattgttggc agcattggcc
     1861 ccaaaaccta gtgcatgaat acccggtctc catgccaaat ttccagccaa tccgcctccg
     1921 gcagcagcag ccagccctgt tttgccgcta ctcctgttgc cgcaccgatt cctgccgcag
     1981 tagccgcgcc ttgcgcagtt cctaatttac catgattata caaattagca ccatgatact
     2041 cccatgcacc taatgcaccg ccaaaagcag cggctgcaat aatgggaaca aattcacctt
     2101 gtgtttcttt catttcagcc tgtgataatt gaattgcttt cacattttgg ctgtcaaaaa
     2161 cttggctgtc taaattttgc gccattacag gtgtaatcat catagccatt acagttgcaa
     2221 ttttcgttgc gctggtttgc acataaatag gattagcaaa ttcgctttga ttgcgttcag
     2281 tgttgatgta gctaatactg ctttctagtt tgaatttacc cttgtcagtc aataattctt
     2341 ccaaacttaa aggtaagtca gcataagcaa tttggctggc aacagtcaaa ataaaatcta
     2401 ttagacattt gtgtttttgc atcatttcgt ttgattttct agtttttgag aatgatacaa
     2461 agttttttac aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat
     2521 tgatagaata aatagcgagc atcgattcac ggtgcacttt agtgcaaagc gtacaacgcg
     2581 agcctgaacc accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg
     2641 tgcaagcgag cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa
     2701 gcaggatagg agcggtaacg caaaggtctc gggctttgat ttcgccgtaa accctgctgc
     2761 cgccttgtcc ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg
     2821 ttttcatttg gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata
     2881 caggcggtgc gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac
     2941 ccctgtttcc gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg
     3001 gcggctttgc ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc tggcgagggt
     3061 gcaggcttcg cggaagccgc agacggcgac ggcattgtgt ttgccttctt caaaaatacc
     3121 cagcaggcgg tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat
     3181 gtcggtcagg gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc
     3241 gccgatttcg cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ctttctcggc
     3301 ggcgtgtttt tcttcgattg cctgtgtcag catgacgtgt tcgtcggcag ggttgttttg
     3361 tccgccctgt tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc
     3421 ggcggcaaag tccatcagga agcggaacat ttggggattg tcggtttcca gttttttatc
     3481 gacggcgacg cagcggatgc tgtccaccag tatggggcgc acccatttcg aataggaaag
     3541 cctgtggtct ttggtgaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg
     3601 acggaaaatc ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac
     3661 taacatggcg gcttcctgaa aagaaatgtc tggctcgatt ataccttatg cttatgcagg
     3721 cgtgtttgga tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca
     3781 aatgtgcgat aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacatacggg
     3841 cggcgtggac gacgtataac cggatacccg cctgacgcgg gttttttacg gaaggggggc
     3901 aaaaatgcct aatccgcttt acagacagca tatcatctcc atttcggatt tgtcgcgcga
     3961 acagttggaa tgcctgcttc agacggcatt gaagctgaag gcgcatccgc gcggcgattt
     4021 gttggaaggc aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca cgaggctgtc
     4081 gtttgaaacg gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg acggcgcgaa
     4141 taccagtgcc aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt ccggctacac
     4201 cgacgcaatc atccaacgcc accccaaaga cggcgcggcg cgcgtggcag cggagttttc
     4261 gcgcgtcccc gtcatcaacg ccggcgacgg caccaaccag caccccagcc agaccttgtt
     4321 ggatctggta acgatttatg aaacgcaggg gcgtttggac aagctcaaaa tcgccatggc
     4381 gggcgacttg aaatacgggc gcaccgtgca ttcgctttgt caggcgttga aacgctgggg
     4441 ctgcgaattt gcctttgttt cgccgcccag cctcgcgatg cccgactaca ttaccgaaga
     4501 gttggacgaa gccggctgcc gataccgtat cctcggcagt ttggaagagg cggcggaatg
     4561 ggcggacatc ctgtatatga cccgcgtcca gcgcgaacgt ttcgatgaac aggaatttgc
     4621 caaaatccaa ggcaaattca acctcgaagc gtctatgctc gcccacgcca aaccgaacct
     4681 gcgcgtgctg caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg atgccacgcc
     4741 gcatgcctat tatttcgagc aggcaaccaa cggcgtttat gcgcgtatgg cgatattgtc
     4801 gctggtgttg aacgaagaag tgtgaggaac cgatatggaa accccgaaac tcagtgtcga
     4861 agccattgaa aaaggtacgg ttatcgacca tattcccgcc ggcagggggc tgaccatcct
     4921 gcgccagttc aaacttttgc actacggcaa cgcagtaacc gtgggcttca acctgcccag
     4981 caaaacccaa ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg acgacaaagc
     5041 cgccgaccgc cttgccctgt ttgcacccga agcagtggtc aacaccatcg acaatttcaa
     5101 ggttgtgcag aaacggcatt tgaacctgcc cgacgaaatc gccgaagtgt tccgctgtcc
     5161 gaacacgaat tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg ttaaaaaaca
     5221 caacgggcag acgcggctga aatgccatta ctgcgaaaaa acctacagcc gggattcggt
     5281 ggcggaagcc tgacggattc ccttaaaccg agcgggcggc atttcgtctg ccgcttgttt
     5341 tgccaatctg aaatggaatg atgatgcacg cttctgtcca aagccgtttc gcaccgatac
     5401 tttatgtttt gattttcttt gccggttttt tgaccgcgca aatctggttc aatcagaaag
     5461 cctatactga agagctgcct ccgcttctgt ccgcattgtc cgccgtcgcg ctggtgtggc
     5521 tggcgtgggc gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc taccgcgaaa
     5581 aaatgataca gaacgaaagc atacaccccg tcctgcacgc ttctttgcaa cacttggaac
     5641 acaagccgca aatgctcgcc ctgctggtca aaaaccacgg caaaggtatg gcggagcagg
     5701 tcaggttcaa ggcagaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt
     5761 tggcaaaaat ggatatgttc gcattgggga cggacgcggt cgcctcgggc gaaacctatg
     5821 gacgcgtgtt cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag
     5881 gaatgttgaa actgacggcg gaatataaaa acatcttcgg cgatgcctgc cgttcggaaa
     5941 cggcgttgga gttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa acatcggaaa
     6001 agtccaaacg gatattttat tgaagatgga aaaatgccgt ctgaaacgga aggtgtttca
     6061 gacggcattt ttgtcggatg attaattatt cggagcggtt gaagccaaac ttcacgcggc
     6121 tgcggccctg atccggtata ttgtccaaat cgcgtcccgg attggcggcg gtgtcgccta
     6181 cggaaatatc ggagatgttt tccaaaatga tggcggacga caggtgttcg gaggtgcggt
     6241 aaaccattgc caagcccact tcttcggcag gagtggaaat cagctcgacg gtatccctgc
     6301 ttttgaaatt gttggagagg tcgacctgca tcgttttctt gcgtttgtag aggctcaaaa
     6361 ccgtgccttt gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt tgaaactggc
     6421 cggcaatcct tgtgccttca aacacggaaa cgattttagc ctgaaccggg cgggacggtt
     6481 cgtgcggcat catgttgaag cggtcggtgt cttccggcat tttcatcagg tagtcgccct
     6541 gctgtatttc ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg gtgcgcagcg
     6601 gggtaatcaa aggatgggtg cgggtatggt attcgttgtc tttcggccgt tctccagcct
     6661 gtttcgagcg ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg atgccgctga
     6721 atgcgacttc ctgcccgagg aatttacccg tatccggatc ggtgatgttt ttattgattc
     6781 ggtaggtcag gtagcgaccc ggctctttca ggcctttggt gtaaaccctg gtgcctttgg
     6841 tgtacagcag cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg gtttctttgc
     6901 gggaaacgat ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg atggcgggaa
     6961 taccgtatcc ggacacttcc ttatccggac tcattttgac gacggggatg ccgtctgtct
     7021 gttccaagcc gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc tggtccggat
     7081 aaatcaggtc gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg ccccattgcc
     7141 acgggctgta caggtatttg cccgaaatgc cccacagggt gtcgccctgt ttgaccgtgt
     7201 agcgttccgg cgcgttcggg cgcacctcca aatttgccgc caaagtttgt gttgagaatg
     7261 ccatacctgc cgcgcagagc agggttataa tacgacgttg cataaccgtt ccccttatct
     7321 gataaatttc ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga
     7381 tgtgccgcgt gataaaaaat gttccgcatt ttaacatcga attatccgca ccatcacggt
     7441 aattatgaaa aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttattgaa
     7501 tatcttgcaa tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagtcga
     7561 cgagcgcatc cggaagctga ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat
     7621 cgggctggcg gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg atttgaccga
     7681 agaccgcagc gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac
     7741 cacttacgaa gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa cccgcgccga
     7801 acgcgtcaag gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg aggcggacgg
     7861 cttgctggcg atttgcgtgc agcacgagtt ggaccacctg atgggcatcg tgtttgtcga
     7921 acgcctgtcg cagctcaagc agggacgcat caaaaccaag ctgaaaaaac gtcagaaaca
     7981 tacgatttga cccttttgcc gtgccgtctg aacgctgcaa agttttcaga cggcacggtc
     8041 ttgtccgaca accttacgca tacgcaggaa tatgctatga aagtcatctt tgccggcacg
     8101 cccgattttg ccgccgccgc cttaaaagcc gttgccgccg ccggttttga aatcccgctg
     8161 gtgctgaccc agcccgaccg tccgaaaggg cgcggtatgc aactgaccgc cccgcccgtc
     8221 aaacaagccg cgctggaact cggtttgcgc gtggcacagc ccgaaaaact gcgtaacaac
     8281 gccgaagccc tgcaaatgct caaagaggtc gaggcagacg taatggtggt tgccgcctac
     8341 ggtttgattc tgccgcagga agtgttggat acgccgaaac acggctgcct caacatccac
     8401 gcttcgctgt taccccgttg gcgtggcgcg gcgccgattc aacgcgcgat tgaagccggc
     8461 gatgccgaga caggcgtgtg tattatgcag atggacatcg gtttggacac cggcgatgtg
     8521 gtcagcgaac accgctacgc catccaaccg accgataccg ccaacgaagt ccacgacgcg
     8581 ctgatggaaa tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc
     8641 cgtctgaacg cggtcaaaca gcccgaagaa ggtgttactt acgcccaaaa attgcgcaaa
     8701 gaagaggcgc gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa aatccgcgcc
     8761 ttcaaccccg tgcctgccgc gtgggttgag tatcagggca agccgatgaa aatccggcgg
     8821 gcggaagtgg tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc ggcggacggt
     8881 ttggtcgttg cctgcggcga aaacgcgctg aagattaccg aattgcagcc tgccggcggc
     8941 aggcgtatga atatcgcggc gtttgcagca ggacggcata tcgaagcagg gacgaagctg
     9001 taaacctttt tcagacggca tttgatccgc aaacgggaat gccgcctgaa accatcagtt
     9061 gaagaaagcg aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc
     9121 attgcggcgg ttgccgaagg acgcaacctt caggacgtgt tggcgcaaat ccgcaccgcg
     9181 catcccgacc ttacggcgca ggaaaacggc gcgttgcagg acatcgccta cggctgccag
     9241 cgttatttgg gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc gattgacaac
     9301 ccgcagctcg aaagcctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg
     9361 ccgcacgccg tggtcaatga ggcggtggaa agcatcgcga aaatcgggcg cgggcagtac
     9421 cgttcgtttg ccaacgcggt tttgcgccgt tttttgcgtg aacgtgaaaa attggcgtct
     9481 tcctgcaaaa aagacgatgt ggcaaaacac aacctgccgc tgtggtgggt ggcttacttg
     9541 aaaaaccatt atccgaaaca ctggcacaac atcaccgccg cgctgcaatc gcatccgccg
     9601 atgacgttgc gcgtcaaccg ccgacacggc aatgccgaaa gctatttgga aaaactggct
     9661 gcggaaggta tcgccgcaaa ggcgttggac gaatatgcgg ttacgttgga agaagccgtg
     9721 ccggtaaacc gtctgcccgg tttttcagac ggcattgttt cggtacagga cttcggtgcg
     9781 cagcaggcgg cgtatctcct taacccgaaa gacggagaac ggattttgga cgcgtgcgcc
     9841 gcgccgggcg gcaagacggg gcatattttg gaactggcgg attgccgcgt taccgccttg
     9901 gacatcgatg caggccgtct gaagcgtgtg gaggacaata tcgcccgttt gggctttcag
     9961 acggcatcgg cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct
    10021 ttcgacgcaa tccttgccga cgtgccgtgc accgcttccg gcgtggcgcg gcgcaatccc
    10081 gacgtgaaat ggctgcgccg tccgaccgac gcgctaaaaa ccgcccgcca gcaggaagcc
    10141 ctgctggacg cactttggca gacgctgaaa cagggcggca gaatgctgct tgccacctgt
    10201 tccgtgttcg tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg ccatgccgat
    10261 gccgaaccga tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac
    10321 gcgcttattc aaaagcatta aacaatggct tgtgctgctg ccgatgcttt ccgttttgcc
    10381 ggacgcggcg gcggagggga tagatgtgag ccgcgccgaa gcgaggataa ccgacggcgg
    10441 gcagctttcc atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt
    10501 gcgccggggc gtgccgctca actttacctt aagctggcag ctttccgccc cgataatcgc
    10561 ttcttatcgg tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact
    10621 gagtttccat ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt cgacagacta
    10681 cgacaccttg gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa
    10741 caaaggcgcg ctgtctggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct
    10801 gtccacttca aaactgccca agccttttca aatcaatgca ttgacttctc aaaactggca
    10861 tttggattcg ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgttttcta
    10921 ccgatcgcag ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc aaccggcagc
    10981 accagttcgc tggcggatta tttctggtgg attgttgcgt tcagcgcaat gctgctgctg
    11041 gtgttgtccg ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcgacggc
    11101 gtattcggtt cgcagattgc caaacgcctt tctgggatgt ttacgctggt tgccgtactg
    11161 cccggcgtgt ttctgttcgg cgtttccgca cagtttatca acggcacgat taattcgtgg
    11221 ttcggcaacg atacccacga ggcgcttgaa cgcagcctca atttgagcaa gtccgcattg
    11281 aatttggcgg cagacaacgc cctcggcaac gccgtccccg tgcagataga cctcatcggc
    11341 gcggcttccc tgcccgggga tatgggcagg gtgctggaac attacgccaa cgggtttaca
    11401 cagttgtccc tttacaatgc cgcaagcggc aaaatcgaaa aaagcatcaa cccgcacaag
    11461 ctcgatcagc cgtttccgga taaggcgcgt tgggaaaaaa tccaacaggc gggttcggtc
    11521 agggatttgg aaagcatagg cggcgtattg tacgcgcagg gctggctgtc ggcgggtacg
    11581 cacaacgggc gcgattacgc cttgtttttc cgtcagccgg ttcccaaagg cgtggcagag
    11641 gatgccgtct taatcgaaaa ggcaagggcg aaatatgctg agttgagtta cagcaaaaaa
    11701 ggtttgcaga cctttttcct ggcaaccctg ctgattgcct cgctgctgtc gatttttctt
    11761 gcactggtga tggcgctgta tttcgcccgc cgtttcgtcg aacccgtcct atcgcttgcc
    11821 gagggggcga aggcggtggc gcaaggcgat ttcagccaga cgcgccccgt gttgcgcaac
    11881 gacgagttcg gacgcttgac caagttgttc aaccacatga ccgagcagct ttccatcgcc
    11941 aaagaagcag acgagcgcaa ccgccggcgc gaggaagccg ccaggcatta tcttgaatgc
    12001 gtgttggatg ggctgaccac gggcgtggtg gtgtttgacg aacaaggctg tctgaaaacc
    12061 ttcaacaaag cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
    12121 cggcacggtt ggcacggcgt ttcggcgcag cagtccctgc ttgccgaagt gtttgccgcc
    12181 atcggcgcgg cggcaggtac ggacaaaccg gtccatgtga aatatgccgc gccggacgat
    12241 gccaaaatcc tgctgggcaa ggcaaccgtc ctgcccgaag acaacggcaa cggcgtggtg
    12301 atggtgattg acgacatcac cgtgctgata cacgcgcaaa aagaagccgc gtggggcgaa
    12361 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
    12421 gaacggctgg cgtggaaatt gggcgggaag ctggatgagc aggatgcgca aatcctgacg
    12481 cgttcgaccg acaccatcgt caaacaggtg gcggcattga aggaaatggt cgaagcattc
    12541 cgcaattatg cgcgttcccc ttcgctcaaa ttggaaaatc aggatttgaa cgccttaatc
    12601 ggcgatgtgt tggcattgta tgaagccggt ccgtgccggt ttgcggcgga gcttgccggc
    12661 gaaccgctga tggtggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
    12721 aaaaatgccg ccgaagcggc ggaagaagcc gatgtacccg aagtcagggt aaaatcggaa
    12781 gcggggcagg acggtcggat tgtcctgacg gtttgcgaca acggcaaagg gttcggcagg
    12841 gaaatgctgc acaacgcctt cgagccgtat gtaacggaca aaccggcggg aacgggattg
    12901 ggtctgcctg tggtgaaaaa aatcattgaa gaacacggcg gccgcatcag tttgagcaat
    12961 caggatgcgg gtggcgcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
    13021 tagcagcgat attttaattg tagacgacga aatcggcatc cgcgacctgc tgtcggaaat
    13081 cctgcaggac gaaggttatt cggtcgcatt ggcggaaaac gccgaagagg cgcgcaagct
    13141 gcgccatcag gcgcgccccg cgatggtgct gctggatatt tggatgcctg attgcgacgg
    13201 catcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtgatgat
    13261 gagcgggcat gccagcatcg ataccgccgt ggaagccacc aaaatcggtg cgctcgactt
    13321 tttggaaaaa ccgatttccc tgcaaaagct gctgtccacg gttgaaaacg cgttgaagta
    13381 cggtgcggcg caaaccgaaa cggggcctgt attcgacaag ctgggcaaca gtgcggcgat
    13441 tcaggaaatg aaccgtgagg taggggctgc ggtgaaacgt gcctctcccg tacttttgac
    13501 gggcgaggca ggttcgccgt ttgaaacggt ggcacgctat ttccataaaa acggtacgcc
    13561 gtgggtcagc ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
    13621 ggcggagggc ggcgttttgt atgtcggcga catagcccaa tacagccgca acatccaagc
    13681 cggcattgcc tttattgtcg gcaaggcaga acgctgccgc gtcagggtgg tcgcatcggg
    13741 cagcagggcg gcaggttcag acggcattgc ctgcgaggaa aagctggcgg aactgctgtc
    13801 ggaatcggtc gtccgtattc cgccgctgcg tatgcagcat gaagatattc ccttcctgat
    13861 acaggggatt gcctgcaatg tggcggaaag ccaaaagatt gcgcccgccg cattcagtga
    13921 agatgcgctt gccgtgctga cccgttacga ctggccggga aatttcgacc aactgcaaag
    13981 cgtcgttgca acgctgttgt tggaggcgga cggacaggaa atcggcgcgg cggcggtttc
    14041 ttcccttttg gggcagaatg tgcctgccga gggagcagaa gatatggtgg gcgggtttaa
    14101 tttcaacctg cccctgcgcg aattgaggga ggaggtggag cggcgttatt tcgagtacca
    14161 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaagtcggtt tggaacgcac
    14221 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
    14281 aaccgaagaa taggcccgga cggccggttt tccggctgcg ggcttttgtt ttcagacggc
    14341 atttggtgca aatgccgtct gaaatcgtaa ggggacggat tttatgacag aggacgaacg
    14401 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
    14461 gatgcgccgt ttcggcagcg cgcaaaatgc cctgtccgca ccggcggaac aggtggcggc
    14521 actgatacgg cacaaacagg cgcttgaggc ttggcgcaat gcggaaaaac gcgctctggc
    14581 gcggcaggcg gcagaagcgg cattggaatg ggaaatgcgg gacggatgcc gtctgatgct
    14641 gcttcaggat gaagattttc ccgaaatgct gacgcagggg ctgaccgcgc caccggtttt
    14701 gtttttgcgc ggcaacgtac aactgctgca caaaccttcc gccgccattg tcggcagccg
    14761 tcatgccacg ccgcaggcaa tgcggattgc caaagatttc ggcaagtcgt tgggcgggca
    14821 aaatatcccc gttgtgtcgg gtatggcttc cggtatcgat actgccgccc atcagggcgc
    14881 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggtatagacc gcatttaccc
    14941 gccgtccaat aaaaaccttg cctatgaaat cgccgaaaaa ggattgattg tcagcgagtt
    15001 tcccatcggt acgcgcccgt atgccggcaa ttttccgcgc cgcaaccgcc tgattgctgc
    15061 cctgtcgcaa gtaacgctgg tggttgaagc cgccttggaa tccggttcgc tgattaccgc
    15121 cggcttggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaatcc
    15181 gcacagcaaa ggctgccaca aactgattaa agacggcgca aaattggtgg aatgcctgga
    15241 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcat catattctat
    15301 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
    15361 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
    15421 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
    15481 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgcaatgcc
    15541 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
    15601 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgt ccgccgcccg
    15661 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
    15721 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaacct gccgacagcg
    15781 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
    15841 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
    15901 acgcgctgat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
    15961 tgctgctttt atgggcaaac aagagcgagc tgcccgtgtt ggtcggcgac gagctgatga
    16021 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcccgccc
    16081 gaggccgtct gaaacgccgg catcaaaacc accatccaga gaacgacaaa tggcgaaaaa
    16141 cctattaatc gtcgaatccc cgtccaaagc caaaaccctg aaaaaatatt tgggcggcga
    16201 ttttgaaatc cttgcatcct acggacacgt ccgcgacctc gtccccaaaa gcggcgcggt
    16261 cgatcccgac aacggctttg cgatgaaata ccaactcatc agccgcaacg gcaaacacgt
    16321 cgatgccatc gtcgccggtg ccaaagaagc tgaaaacatc tacctcgcca ccgacccgga
    16381 tagggaaggc gaagccattt cctggcatct tttggaaatc ctcaaatcca aacgcggctt
    16441 gaaaaacatc aagccgcagc gtgtcgtgtt ccacgaaatc accaaaaacg ccgtgctcga
    16501 tgccgttgcc catccgcgcg aaatcgaaat ggacttggtc gatgcacaac aagcccgccg
    16561 tgctttggac tatttggtcg gctttaacct ttcgccattg ttatggaaaa aaatccgccg
    16621 aggtttgagc gcggggcgtg tgcaaagccc tgctttgcgc ctgatttgtg agcgcgaaaa
    16681 cgaaatccgc gcgtttgaag cgcaggaata ttggacggtg catctcgaca gccacaaagg
    16741 ccgcagcaag ttcaccgcca aactcgccca atacaacggc gcgaaactcg aacaattcga
    16801 cctgtcgaac gaagccgcgc aagccgacgt attgaaagaa ctcgaaggca aagaagccgt
    16861 cgttaccgcc attgaaaaga aaaagcgcag ccgcaatcct gccgcgccgt tcaccacttc
    16921 taccatgcag caggatgccg tgcgcaaact cggcttcacc accgaccgca ccatgcgtac
    16981 cgcccaacag ctttatgaag gtatagacgt agggcagggc gcgatcggtc tgattaccta
    17041 tatgcgtacc gacagcgtga atttggcgga tgaagcgtta accgaaatcc gccattacat
    17101 tgaaaacaaa atcggcaaag aatatctgcc gagtgccgcc aaacaataca aaaccaaatc
    17161 caaaaacgcc caagaagccc acgaagccat ccgtccgact tccgtgtacc gcacgcccga
    17221 aagcgtcaaa cccttcctga gcgcagacca gttcaaactc tatcaaatga tttggcagcg
    17281 taccgtcgcc tgtcagatga cgcctgccaa attcgaccaa accaccgtcg atattaccgt
    17341 cggcaaaggc gtattccgcg taaccggaca agtgcaaacc ttcgcaggct tcttgagcgt
    17401 ttatgaagaa agcagcgacg atgaagaagg cgaagacagc aaaaaactgc ccgaaatgag
    17461 cgaaggcgat aaattgcccg tggacaaact ctacggcgaa caacacttta ccactccgcc
    17521 gtcgcgctac aacgaagcca cgctggttaa agccctcgaa gaatacggca tcggccgccc
    17581 ctcgacctac gccagcatca tctccacgct caaagaccgc gaatacgtta cccttgagca
    17641 aaaacgcttc atgcccaccg acacaggcga catcgtcaac aaattcctga ccgaacactt
    17701 cgcccaatac gtcgattacc acttcaccgc caaactcgaa gaccagcttg acgaaattgc
    17761 cgacggcaaa cgccaatgga ttcccttgat ggacaaattc tggaaaccgt tcatcaaaca
    17821 agtggaagaa aaagaaggca tcgaacgcgc caaatttacc acgcaggaac ttgatgaaac
    17881 ctgcccgaaa tgcggcgaac acaaactgca aatcaaattc ggcaaaatgg gtcgttttgt
    17941 tgcgtgtgcc ggttatcccg agtgcagcta cacgcgcaat gtcaacgaaa ccgccgaaga
    18001 ggccgccgag cgcatcgcca aagccgaagc cgaacaggcc gaactcgacg gacgcgaatg
    18061 tcctaaatgt ggtggtcgcc tagtgtacaa atacagccga accggcagca aattcatcgg
    18121 ctgcgccaac tatccgaaat gcaaacacgt cgagccgctg gaaaaaccga aagataccgg
    18181 cgtccagtgt ccgcaatgca aaaaaggcaa cctcgtcgag cgcaaatccc gctacggcaa
    18241 actgttttac agttgcagca cctatcccga ctgtaactac gccacatgga acccacccgt
    18301 tgcagaaagc tgtccaaaat gcgcatggcc ggttttaacc atcaaaacca ccaaacgctg
    18361 gggcgtcgaa aaagtctgcc cgcaaaaaga atgcggctgg aaagaacaga ttgaaccgcc
    18421 tgcgccgaaa gagtgagatt aggttggttt gaaagagaaa aggtcgtctg aaaaattttc
    18481 agacgacctt tgcttttctg tgattggttt atttgaatcc gcgtgttgtt ttaaagtccg
    18541 ataaaatccg gttcatttca ggcgcaaaca aggcgatgta atcgtaagat agaccgcgac
    18601 tggcactggg atggggaaag cagacgactt cgcaatcttc aaacgattgg aatttgacat
    18661 tgaaacgtgt accgtcaaat tctttttgca ccgtctccag cggtttggtc tgcttaccga
    18721 ccaactgctc gaagcgtggc agtacatttt ggttgttcag aaaatccgcc aacctgctgc
    18781 ccatgaagag gatgactttc ggacgcagtt tttcgatgtg gtagagaaaa ttatcgatgt
    18841 gctcgggttg tgtgaacttg tcgggattgt cgatagtgtt gccctgtgta gcagcccagt
    18901 tggtttgaac cagggatttt tcaaatgcac cgcccaatcc attttcgtct aaggggtgtc
    18961 cccacatttc aaaccaattt tttatcgtat tgtcgtaacg ccactttttt gcctgctctc
    19021 cgaaatagag ggatttgttt gcaaatgtat ggtcgatttt gttttcaggg agtttgtatt
    19081 cacctgctac ataagcagcc tcatcggctt tactccaacc ccattcatag ccacaaatca
    19141 ttaagccatg tttgtcgttg tagcctttga acaggctgtt gctcaaattc aaatccttca
    19201 tcatgaactc ttccttttaa aatttaagag cgattgactt caatgttttt agatggggtg
    19261 gaaaaatcct tgtgtaggca acataaattc aataaatttc ttgataattc gaaacctact
    19321 aatagcgcac ctataaaagc tttttcatta cgttcagcat gacggtcacg tcgttcatat
    19381 tttttacgct tgctgttccc tgttattaca gctaagccaa gtgatatggc gagaattgcc
    19441 caaacaatag tacttaataa caattttccc catactatca ataaggaaag aaaaaacctt
    19501 ttagtattag atcgataggt tataatccat gcccatgaaa atgcaataag agttatacat
    19561 aagatacaag ctgacaggat ttctttattt ttaattaaat aacttagagc gatgaggatg
    19621 acaggtgtca agaaaataat agttacatcc cgatagctat aaaagaaaac tgccctattt
    19681 tgaatgtgga gatgtgcaca gaatccaata tagctaagga taatagttaa tataataaaa
    19741 aaagaccacc aagggtgaag agataggaat tccatgtttt cccttttttt gtaaaaagga
    19801 aaaaatctta tctaatagtt aattgcctaa ccagccagaa gaagtttaaa atctatccca
    19861 ataattcaac catctataca gaaagttcag cttatggaaa cccacgaaaa aatccgcctg
    19921 atgcgcgaat tgaataaatg gtcccaggag gatatggcgg aaaagctggc gatgtcggca
    19981 ggagggtatg ccaaaatcga acggggcgaa acgcagttga atatcccgcg tttggagcag
    20041 ttggctcaga ttttcaaaat cgatatgtgg gacttgctca aatcgggcgg tggtgggatg
    20101 gtgtttcaga ttaatgaagg tgatagtggt ggcgatattt gcgttgtatg cgtcgggtga
    20161 tgtttcgatg aaaatagaat ttttaaaaat ggagttgaaa cactgcaaag aaatgttgga
    20221 acaaaaagac aaagaaatcg agctgctccg caagctgacc gaaaccgttt aaacagatat
    20281 gccgtctgaa aaaagttttc agacggcata ttctttgaca ggtcttgtat aataccgttt
    20341 gaacttacag gtttttgatt atggcggcag gcaaacatac caaacacagc aaccgggtac
    20401 gcattatcgg cgggcaatgc cggggcagga aattgagttt cacatccgcc gacggactgc
    20461 gtccgacacc cgacagcgtg cgtgaaaagc tgtttaactg gctgggacag gatttgacgg
    20521 gtaaaacggt tttggatctc ttcggaggca gcggcgcact cggtatagaa gccgcttcgc
    20581 gcaacgccaa acgcgtgctg atttcggata acaaccgcca aaccgtgcag accttgcaga
    20641 aaaacagtcg tgaactgggt ttggggcagg tgcaaatcgt ctgttcagac ggcatcgcat
    20701 atttgaagac cgtatccgaa cagtttgatg ttgtctttct cgacccgccg tttgcatggc
    20761 aggactggca aatcctgttt gatgccttga agccgtgcct gaacccccgg gcattcgtct
    20821 atctcgaggc gggtacgctg ccggatattc ccgattggct gacggaatat agagagggga
    20881 aatcggggca gagtacattt gaattaaggg ttttccaagt ggctgaataa tatgcgcttt
    20941 gataatcatt tccgagttgt aaacattcgt ttgcaaccgt ccggttcaaa aaaaccttgt
    21001 gctataatcc gcgcccgccc ggttttgata atttagtgga aaaggaaaag aaatgtcgct
    21061 ttttattacc gacgagtgca tcaactgcga cgtatgcgaa cccgaatgcc ccaatgatgc
    21121 catttcccaa ggcgaggaaa tttacgaaat caaccccaac ctctgcacgc agtgcgtcgg
    21181 acactacgat gagccgcagt gccagcaggt ttgcccggtg gactgcatcc tgattgacga
    21241 agaatatccc gaaacccatg acgagttgat ggcgaaatac gaaaagatta tccagtttaa
    21301 ataaattctt tttaaaacat caaattatgt ctgttttgaa ataaaatcaa aaaaaaactt
    21361 gacggaaaag caagccgcta ataaactaac gttctctttt ggaggggttc ccgagcggtc
    21421 aaagggggca gactgtaaat ctgttgcgaa agcttcgaag gttcgaatcc ttctccctcc
    21481 accaaaattc ttacttgggg cagtagcgag taatgcgggt gtagctcaat ggtagagcag
    21541 aagccttcca agcttacggt gagggttcga ttcccttcac ccgctccaaa caattaggcc
    21601 catgtagctc aggggtagag cactcccttg gtaagggaga ggccggcagt tcaaatctgc
    21661 ccatgggcac catctctcga ttattcattt ctttaaggct tagatatata ggatattg
//
LOCUS       DAWFKN010000027        20672 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFKN010000027 DAWFKN010000000
VERSION     DAWFKN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.027"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(110..538)
                     /locus_tag="WGR46_001032"
     CDS             complement(110..538)
                     /locus_tag="WGR46_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0855986.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WGR46_001033"
     CDS             complement(603..2351)
                     /locus_tag="WGR46_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0855987.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WGR46_001034"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WGR46_001034"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0855988.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WGR46_001035"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WGR46_001035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0855989.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WGR46_001036"
     CDS             4754..4924
                     /locus_tag="WGR46_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0855990.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WGR46_001037"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WGR46_001037"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0855991.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WGR46_001038"
     CDS             6085..6279
                     /locus_tag="WGR46_001038"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0855992.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WGR46_001039"
     CDS             6481..7890
                     /locus_tag="WGR46_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0855993.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="WGR46_001040"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="WGR46_001040"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0855994.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEYPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILVEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGR46_001041"
     CDS             complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGR46_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0855995.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13173..13385
                     /locus_tag="WGR46_001042"
     CDS             13173..13385
                     /locus_tag="WGR46_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0855996.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(13664..14296)
                     /gene="mtrR"
                     /locus_tag="WGR46_001043"
     CDS             complement(13664..14296)
                     /gene="mtrR"
                     /locus_tag="WGR46_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0855997.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            14704..15942
                     /gene="mtrC"
                     /locus_tag="WGR46_001044"
     CDS             14704..15942
                     /gene="mtrC"
                     /locus_tag="WGR46_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0855998.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            15954..19157
                     /gene="mtrD"
                     /locus_tag="WGR46_001045"
     CDS             15954..19157
                     /gene="mtrD"
                     /locus_tag="WGR46_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0855999.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19212..20615
                     /gene="mtrE"
                     /locus_tag="WGR46_001046"
     CDS             19212..20615
                     /gene="mtrE"
                     /locus_tag="WGR46_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0856000.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPNDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
BASE COUNT         5018 a         5088 c         5576 g         4990 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgt tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaccgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat caggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag
     7261 gttaaggaag tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac
     7561 ccgttcggca aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg
     7621 agcgtgttgg cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa
     7741 atcaagctgc ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc
     7801 ccgaccgcgc tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctaggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctt gtctatcgtc cgcagtggat agacgcgtgg cagcagggca
     8461 ggatactcag tttgggcgac gatgaaatct ggcagtccaa actgtggcgt tatcttgatg
     8521 acggcaggca gagcgcgccg caccgtgttg cgttgtggga aaagctgttg gaatctttga
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaac attgcgatgt gttcgtgttt gcactcaatc
     8701 cgagtgggat gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc ggtacggatg acggttcgat acgtatcgtc tccgcgcaca
     9001 gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaatatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa tatcgaatcc tatacgcctt
     9121 ttatcgaggc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cggacgtgaa aataagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgacatg gttgccgatt
     9361 tgaacgtccg ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaatccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg ttcggacgtt
     9541 ttgccgcctt cctcgaaacc ctgtcggata ttgtacggat atggcggcaa cctgcgacgg
     9601 ttgatgaatg ggtaggacgt tgccgaggtt tgcttgaaac attgttccaa gccgaagccg
     9661 atgaccaaaa ggcagtccaa aacctcgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccac atccgccgct
     9781 ttctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccc tttaaagtca tctgtctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgcgcccg ccgcgacgac gaccgctacc tcttcctcga agccctcatc agcgcgcgtg
    10021 aaatcctgta cctgtcctac atcgggcgcg acatccgcaa agacgaagaa ctggctccgt
    10081 cttctttgtt gggcgaactc atcgataccg ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaacctgata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc gccgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgatcaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gcttctggcg caaccctgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagcc gcaacacgcc gatcaaatcg ccgaaatcta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag caggacttcc aaaccgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag tcccaaactt cctccgctgc cctacgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttttgt tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 tggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaaaacctc gcttgccacc gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc taccacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc gctcgaacag cccctgtttg aaaacctcgt ccgtctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaaag gaagaggccg gagaagcctg acgggatgga aaggcaaatg
    11221 ccgtctgaaa acagcaaaaa ggttttcaga cggcattttg tttatcgtgt ttaaggtgcg
    11281 ggatagaatg tgggcgcgcc gggaccgacg ggcaggccca aaacaaatac ccaaatgcag
    11341 aataaggcaa tccacgcaat caagaagaaa gcggaatacg gcaacatcat agaaatcagc
    11401 gtacccacgc ccgcatcttt tttgtatttg atcaccgtcg ccataatcag cccgaaataa
    11461 ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc ggcttgaatg
    11521 acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc agttaccgcc
    11581 cattgcgcgg aggcggagcc tatcatcaga ttgataaaag cacaaattaa aataaaaccg
    11641 ataaacaaca cgctgccgcc caagccgact tcttttaaga acgtcgcccc tttaacggca
    11701 atatattgcc caatattcgt ccaattaaaa aatgcgacaa actgtgcggc aaaaaagatg
    11761 atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac gacttcctgt
    11821 tcgccgcgca aacttcgggt tacccggcca taaacaatgc ccggcagtgc aaacaacaag
    11881 aaaataaaaa caacaatcga ttttaaaaac ggcgaaccgg aaaccaatcc tgtttcagga
    11941 tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga taaggcaaca
    12001 aacaccacgc cagcccaaat taatccttta tattccaaag gcgtgatttc attggaatgc
    12061 cgaaagtctt tttcttcttg tgacaaatct gattgataag ggcccaattg cggttcgacg
    12121 attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggc taccataaaa
    12181 aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc cgcctgttgg
    12241 gtgatgcctg ccaagagcgg atcgattgtg cctaagaaca gattggccga ataaccgccc
    12301 gaaacgccgg cgaaagccgc agccagaccg gcaagcggat ggcggccgag ggaatgaaag
    12361 atgatggcgg acaaagggat taggacgaca tagcccaatt cagaagcggt attagataaa
    12421 atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gagattttgt gagcaataag
    12481 cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa taaagaaacc
    12541 aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt caggattttg
    12601 atcaaaccgt cagcatcgag caggctgaca acgtgaatca aaccgtcatc ggcacgtcct
    12661 ttcgcaccaa cagggcgcgg atcggggacg gatagtccga aatacgcacc ggcggcagag
    12721 gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg caacatattg
    12781 cccagccatt cgactgtgcg taaaaatcgt ccgtcccgtt gcgtatcggt ttgactcatc
    12841 tgcttctcct taaaaaaaat atattgagtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgtatatg tatataaccg taatagatta aaacagaaaa
    12961 tgcccacatc gttattctca taaaggcggt aatccgaaaa cttgacgtta aagccttatc
    13021 aggaatgact ggaactcaac aaagcggttt cccgctttcg cggaaatggc gggattttcg
    13081 gtttttgttt gcaaaaatgc cggagaaaaa tgcagtttac attttgaatg gtattgttat
    13141 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaattatgg
    13201 acaaatatga tccatggcat gaagatgatt ttgagtcgta tgaagacatt gccaaagatg
    13261 tatcgttgat gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13321 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13381 tttagtgaat atgagacctt tgcaaaaata gtctgttaac gaaatttgac gcataaaaat
    13441 gcgccaaaaa attttcaatt gcctaaaacc ttcctaatat tgagcaaaaa gtaggaaaaa
    13501 tcagaaaagt tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta
    13561 ttgcaaaggt ctcaatatgt atttttagaa gaaggctttg tgtcattttg atgccgtctg
    13621 aaagggtttg ttcgtttcag acggcattgt taccaaggct tgattatttc cggcgcaggt
    13681 cgggatggtt ttccaagttg tccatcatta tcccgatgat gcgcggggcg gttttgccca
    13741 aatcgaaacg ttcgcaggaa gagaaccacc gccaaatcag cccgtccaag gttgatttga
    13801 tgaagatgac cgctgtttcc ttgtccaaat cgtcagccaa atcctgattt tccaccgctt
    13861 cggtcaaaac ggcggtaatt ttctcgcgcc agattgcctg atgcttgcgg gcaatggcga
    13921 taacggcggc gttttgttcc gtgtgttcgc attttaaaaa caggatgttg tggaatttgt
    13981 agtagatgtc gttgctttgc agccgctcga aaaagtgcag cagcgtgtgg cggaataccg
    14041 cccaagaccc tccttcggca tcttcggcat cttgcgcgat gcagttttcg atgtcgtcgc
    14101 agatacgttg gaacagcgcg tcgaacaagt cttccttatt tttgaaatgc caatagagcg
    14161 cgccgcgcgt tacgccggcg gcttgggcga tttcgttgag cgaggtgcgc gcaatccctt
    14221 tgcggtaaaa ggtttccaag gcggcaagca tcaggtgttc tttggttttc aaggcttcgg
    14281 ttttggtttt tctcataatg gcgttttcgt ttcgggtcgg tttgatgagg gcggattata
    14341 aaaaagactt tgtaaccatg caatcgtgta tgtataatga aacccatgaa attgagacta
    14401 catctcaact ttgaaaacag gcataacctg ctttgcaacc cgttcgaaca ttcgggtatc
    14461 aaagcaggcg gtttttatat cctgaaatat agtggattaa caaaaatcag gacaaggcga
    14521 cgaagccgca gacagtacag acagatagta cggaaccgat tcacttggtg cttgagcacc
    14581 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
    14641 actataagaa tgcctgttga ttggcaaaac cctttacatc aaaccagaca gataggataa
    14701 ataatggctt tttatgcttt taaggcgatg cgtgcggccg cgttggctgc cgccgttgca
    14761 ttggtactgt cgtcttgcgg taaaggcgga gacgcggcgc agggcgggca gcctgctggt
    14821 cgggaagcac cggctcccgt cgtcggtgtc gtaaccgtcc atccgcaaac cgtcgcattg
    14881 accgtcgagt tgccggggcg tttggaatcg ctgcgtaccg ccgatgtccg cgcccaagtc
    14941 ggcggcatca tccaaaaacg cctgttccaa gaaggcagtt atgtccgtgc cggacagccg
    15001 ctgtatcaga tcgacagttc cacttatgaa gcaggtctgg aaagcgcacg cgcgcaactg
    15061 gcaacggctc aggcaacgct tgccaaagcg gatgcggatt tggcgcggta caagcctttg
    15121 gttgccgccg aagccgtcag ccggcaggaa tacgatgctg cggtaacggc gaaacgttct
    15181 gccgaggcag gtgtcaaagc agcacaggcg gcaatcaaat ctgccggcat taatctgaac
    15241 cgttcgcgca ttaccgcgcc gatttccggc tttatcggtc agtccaaagt ttccgaaggt
    15301 acgctgttga atgcgggcga tacgaccgtg ctggcaacca tccgccaaac caatccgatg
    15361 tatgtgaacg ttacccagtc tgcatccgaa gtgatgaaat tgcgccgtca gatagccgaa
    15421 ggcaaactgc tggcggcgga tggtgtgatt gcggtcggca tcaaatttga cgacggcaca
    15481 gtttaccctg aaaaaggccg cctgctgttt gccgatccga ccgtcaacga atcgaccggt
    15541 cagattaccc tgcgcgccgc cgtaccgaac gatcagaata tcctgatgcc cggcctgtat
    15601 gtgcgcgtgc tgatggacca agtggcggtg gataacgcat tcatcgtgcc gcagcaggcg
    15661 gtaacgcgcg gtgcgaaaga taccgtgatg attgtgaacg cccaaggcgg tatggaaccc
    15721 cgcgaggtaa cggtcgcgca acagcagggt acgaattgga ttgttacgtc gggtctgaag
    15781 gacggggaca aggtggttgt ggaaggcatc agtatcgccg gtataacggg tgcgaaaaag
    15841 gtaacgccca aagaatgggc gccgtctgaa aaccaagccg ccgcgcctca atccggcgtt
    15901 cagacggcat ctgaagccaa aactgcttct gaagcggaat aaggaaggca tcgatggcta
    15961 aattttttat cgaccgcccc attttcgcgt gggttatttc gattttcatt attgcggcgg
    16021 gtattttcgg cattaaaagc ctgcctgttt cgcaatatcc gtctgtcgcc gccccgacca
    16081 tcaccctgag ggccacttat ccgggcgcgt ccgcgcaggt aatggaagac agcgtgcttt
    16141 ccgtgatcga gcggaatatg aacggcgtgg aaggtttgga ttatctgtcc acttctgccg
    16201 attcgagcgg cagcggcagc gtgagcctga cctttacgcc cgataccgac gagaatctgg
    16261 cgcaggtgga agtgcagaac aagctttccg aagtattgag cacgctgccg gcaaccgtcc
    16321 agcaatacgg cgtaaccgta tccaaggcgc gttccaattt cctgatgatt gtgatgcttt
    16381 cgtcggatgt gcagtcaacc gaagagatga acgactacgc gcagcgtaat atcgttcccg
    16441 agttgcagcg tatcgaaggc gtggggcagg tacgcctgtt cggcgcgcaa cgcgcgatgc
    16501 ggatttgggt tgatcctaag aaactgcaaa actacaattt gtcgtttgcc gatatcggca
    16561 gtgcgctgtc cgcccagaac gtccagattt cagcgggttc tatcggttcg cttcccgccg
    16621 ttcgcggaca gacggttacg gctaccgtaa cggcgcaagg gcagttgggt acggcagaag
    16681 aattcggcaa cgtcatcctc cgcgccaata ccgacggttc taatgtttac ctgaaggatg
    16741 tggcaagggt cggactgggt atggaagact attcttcctc aacccgtctg aacggtgtaa
    16801 ataccaccgg tatggcggtg atgctgtcca acagcggcaa tgcgatggcg acggcaaagg
    16861 cggttaaaga acgcatggcg acgttggaaa aatactttcc tcagggtatg agctggaaaa
    16921 ccccttacga tacttccaaa ttcgtcgaaa tttcgattga aaaagtgatt catactttaa
    16981 tcgaagcgat ggtgctggtg tttgtcgtaa tgtatctctt cctgcaaaac atccgttata
    17041 cgctgattcc gaccatcgtc gtaccgattt cgctgttggg cggtttcgcc ttcatctctt
    17101 atatgggcat gtcgattaac gtactgacca tgtttgcgat ggttttggtc atcggcatcg
    17161 tggtcgatga cgcgattgtg gtggttgaaa acgtcgagcg cattatggcg ggtgaaggct
    17221 tgccgcccaa agaagcgacc aaaaaagcga tgggtcagat ttcgggcgcg gtcatcggta
    17281 ttaccgccgt tctgatttcc gtgttcgtac cgttggcgat gttcagcggg gcgacgggca
    17341 atatttacaa acagtttgcc ctgacgatgg cgtcatcaat cgcattctcc gccttccttg
    17401 cgctgaccct tacccctgct ttgtgtgcca caatgttgaa gacaatcccg aaagggcatc
    17461 acgaagagaa aaaaggtttc ttcggctggt ttaacaagaa attcaacagt tggacgcacg
    17521 gttacgaagg ccgggttgcc aaagtgctgc gtaagacttt ccgcatgatg gttgtctata
    17581 tcggcttggc ggttgtgggc gtgttcctgt ttatgcgcct gccgacttca ttcctgccga
    17641 ccgaagacca aggcttcgtc atggtcagcg tgcaactgcc tgcaggagcg acccaagagc
    17701 gcaccaatgc gactttggcg caagttaccc aactggcgaa aagcattcct gaaatagaaa
    17761 acatcattac cgtttccggc ttcagctttt cgggcagcgg tcagaatatg gcgatgggtt
    17821 ttgccatatt gaaagattgg aacgagcgta ccgcgcccgg cagcgatgcc gttgcgattg
    17881 ccggcaagct gacgggtatg atgatgggga cgcttaaaga cggttttggc atcgccgtcg
    17941 tcccgcctcc gattctggag ttgggcaatg gttcgggcct gagcatcaac ctgcaagacc
    18001 gcaataatac cggccatacc gcattgctgg cgaagcgcaa cgagttgatt cagaaaatgc
    18061 gtgccagcgg tttgtttgac cccagcaccg tccgtgctgg cggtttggaa gactcgccgc
    18121 agttgaaaat cgacatcaac cgtgccgccg cggcggcgca aggcatttcg tttgccgaca
    18181 tccgcaccgc attggcaagt gcgctgagtt cgtcttatgt cagcgacttc ccgaaccaag
    18241 gtcgtctgca acgcgtgatg gtgcaggccg acgaggatgc ccgtatgcag cctgccgata
    18301 ttttgaacct gaccgtgccg aacaaatccg gtgtcgccgt accgctttcc accattgcta
    18361 ctgtttcttg ggaaaacggt acggaacaga gcgtacgctt caacggttat ccttcgatga
    18421 agctgtccgc ttcgcctgca accggcgttt ccaccgggca ggctatggcg gcggttcaaa
    18481 aaatggttga cgaattgggc ggcggttaca gcttcgaatg gggcggacag tcgcgcgaag
    18541 aggcaaaagg cggctcgcaa accctgattt tgtacggttt ggcggttgca gccgtatttt
    18601 tggtacttgc cgcgctttat gaaagctggt cgattccgct ggcggtcatc cttgtgattc
    18661 cgttgggttt gatcggtgcg gctgcgggcg taaccgggcg caacctgttt gaaggactgt
    18721 tgggcagcgt tccctcgttc gccaacgaca tctactttca agtcggtttc gttaccgtga
    18781 tgggtttgag tgcgaaaaac gcgattttga ttatcgagtt tgccaaagac cttcaagcgc
    18841 aagggaaaag cgcggtcgaa gccgctttgg aagccgcccg cctgcgtttc cgtccgatta
    18901 tcatgacctc gttcgccttt attttgggcg tggttcccct gtatattgcc ggcggtgcaa
    18961 gttctgccag ccagcgcgcc attggtacaa ccgtattctg ggggatgctc atcggcacgc
    19021 tcttgtccgt gttccttgtt ccgcttttct atgtggtggt gcgcaaattc ttcaaagaaa
    19081 ctgcacacga acacgaaatg gcagtaaaac acgccgccga agcgggcatt accggttcgg
    19141 acgacagcca acattaagca accatgccgt ctgaacgccc acgggttttc agacggcatc
    19201 aggacttttt tatggatact acattgaaaa ctaccttgac ttctgttgca gcagccttcg
    19261 cattatccgc ctgcaccatg attccccaat acgagcagcc caaagtcgaa gttgccgaaa
    19321 cgtttaaaaa cgataccgcc gacagcggca tccgcgccgt cgatttgggt tggcacgact
    19381 attttgccga cccgcgcctg caaaagctga tcgacatcgc actcgagcgc aataccagtt
    19441 tgcgtaccgc cgtattgaac agcgaaatct accgcaaaca atacatgatt gaacgcaaca
    19501 accttttgcc cacgcttgcc gccaatgcga acggctcgcg ccaaggcagc ttgagcggcg
    19561 gcaatgtcag cagcagctac aatgtcggac tgggtgcggc atcttacgaa ctcgacctgt
    19621 tcggacgcgt ccgcagcagc agcgaagcag cactgcaagg ctatttcgcc agcaccgcca
    19681 accgcgatgc ggcacatttg agcctgattg ccaccgttgc caaagcctat ttcaacgaac
    19741 gttatgccga agaagcgatg tctttggcgc agcgtgtttt gaaaacgcgc gaggaaacct
    19801 acaagctgtc cgaattgcgt tacaaggcag gcgtgatttc cgccgtcgcc ttacgccagc
    19861 aggaagccct catcgaatcc gccaaagccg attatgccca tgccgcgcgc agccgcgagc
    19921 aggcgcgcaa tgccttggca accttgatta accaaccgat acccaacgac ctgcccgccg
    19981 gtttgccgtt ggacaagcag ttttttgttg aaaaactgcc ggccggtttg agttccgaag
    20041 tattgctcga ccgtcccgac atacgcgccg ccgaacacgc gctcaaacag gcaaacgcca
    20101 atatcggtgc ggcacgcgcc gcctttttcc catccatccg cctgaccgga accgtcggta
    20161 cgggttctgc cgaattgggt gggttgttca aaagcggcac gggcgtttgg tcgttcgcgc
    20221 cgtctattac cctgccgatt tttacctggg gtacgaacaa ggcgaacctt gatgtagcca
    20281 agctgcgcca acaggcacaa atcgttgcct atgaatccgc cgtccaatcc gcatttcaag
    20341 acgtggcaaa cgcattggcg gcgcgcgagc agctggataa agcctatgac gctttaagca
    20401 aacaaagccg cgcctctaaa gaagcgttgc gcttggtcgg cctgcgttac aagcacggcg
    20461 tatccggcgc gctcgacttg ctcgatgcgg aacgcagcag ctatgcggcg gagggtgcgg
    20521 ctttgtcggc acaactgacc cgcgccgaaa accttgccga tttgtacaag gcactcggcg
    20581 gcggattgaa acgggatacc caaaccgaca aataaccggt cgggcaataa aatgccgtct
    20641 gaaacgataa ggtttcagac ggcatttctt tc
//
LOCUS       DAWFKN010000028        20647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFKN010000028 DAWFKN010000000
VERSION     DAWFKN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.028"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(353..1495)
                     /locus_tag="WGR46_001047"
     CDS             complement(353..1495)
                     /locus_tag="WGR46_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856001.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASVANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1799..2062
                     /gene="rpsT"
                     /locus_tag="WGR46_001048"
     CDS             1799..2062
                     /gene="rpsT"
                     /locus_tag="WGR46_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0856002.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2222..3370
                     /locus_tag="WGR46_001049"
     CDS             2222..3370
                     /locus_tag="WGR46_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0856003.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3513..4214
                     /locus_tag="WGR46_001050"
     CDS             3513..4214
                     /locus_tag="WGR46_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0856004.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            4272..6080
                     /gene="aspS"
                     /locus_tag="WGR46_001051"
     CDS             4272..6080
                     /gene="aspS"
                     /locus_tag="WGR46_001051"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0856005.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            6231..6515
                     /locus_tag="WGR46_001052"
     CDS             6231..6515
                     /locus_tag="WGR46_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0856006.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6682..8574
                     /gene="speA"
                     /locus_tag="WGR46_001053"
     CDS             6682..8574
                     /gene="speA"
                     /locus_tag="WGR46_001053"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0856007.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8673..9596
                     /gene="speB"
                     /locus_tag="WGR46_001054"
     CDS             8673..9596
                     /gene="speB"
                     /locus_tag="WGR46_001054"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0856008.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9794..11260
                     /locus_tag="WGR46_001055"
     CDS             9794..11260
                     /locus_tag="WGR46_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0856009.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11467..12564)
                     /locus_tag="WGR46_001056"
     CDS             complement(11467..12564)
                     /locus_tag="WGR46_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0856010.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGKGNLRLGN"
     gene            12628..12765
                     /locus_tag="WGR46_001057"
     CDS             12628..12765
                     /locus_tag="WGR46_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0856011.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            12787..13929
                     /gene="bioF"
                     /locus_tag="WGR46_001058"
     CDS             12787..13929
                     /gene="bioF"
                     /locus_tag="WGR46_001058"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0856012.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNIYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRQEVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13939..14586
                     /locus_tag="WGR46_001059"
     CDS             13939..14586
                     /locus_tag="WGR46_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0856013.1"
                     /translation="MKTKFYDCQGEHLILYFAGWGTPPDAVNHLILPTNHDLLICYDY
                     QDLRLDFDFSSYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLICWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            14574..15356
                     /gene="bioC"
                     /locus_tag="WGR46_001060"
     CDS             14574..15356
                     /gene="bioC"
                     /locus_tag="WGR46_001060"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0856014.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            15576..17012
                     /locus_tag="WGR46_001061"
     CDS             15576..17012
                     /locus_tag="WGR46_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0856015.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            17015..18112
                     /locus_tag="WGR46_001062"
     CDS             17015..18112
                     /locus_tag="WGR46_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0856016.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            18124..18660
                     /locus_tag="WGR46_001063"
     CDS             18124..18660
                     /locus_tag="WGR46_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0856017.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            18662..19306
                     /locus_tag="WGR46_001064"
     CDS             18662..19306
                     /locus_tag="WGR46_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0856018.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            19329..19688
                     /locus_tag="WGR46_001065"
     CDS             19329..19688
                     /locus_tag="WGR46_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0856019.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19781..19856
                     /locus_tag="WGR46_001066"
     tRNA            19781..19856
                     /locus_tag="WGR46_001066"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19814..19816,aa:Asn,seq:gtt)
     gene            complement(20355..>20647)
                     /locus_tag="WGR46_001067"
     CDS             complement(20355..>20647)
                     /locus_tag="WGR46_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856020.1"
                     /translation="SKTYSSDLIKTVDLTHIPTGAKARINAKITASISRAGVLAGGGK
                     LARLGAKFSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT         5170 a         4832 c         5331 g         5314 t
ORIGIN      
        1 aggtttccgt gcggacgcgt tcggattcac gactgcgtgg gaatgacggg atttcagttt
       61 gcggattgat ttgaagttgc aaaatcccaa cggatcggat tttcgctttc gcgtttcaaa
      121 gttacggcgt tatcggaaaa acagaaaacc aaagctgcga ggatttattt aaaacaaccg
      181 aatttcaacg aaccggattt tcgcctgcgc caggatgacg gcggaaggtt ttttgtcttt
      241 tctgacagat gtccgcaatc tgacatcccg accgtgggag cgacggttct atttcaatcc
      301 aatataaaat gccgtccgaa cgatgttcgg acggcatttt taatgcttca aatcagttgg
      361 tgccgacttt gattttctgc cacaggctga cagacagttt tttcgcatcc gtgctcattt
      421 gcggcatcac aaaaccgtct ttcatatcct gctcgttcgg gaagatggaa cgggtgttca
      481 ccagctcggc aggcattttt tcgcgcgccg gtttgctggc gggggcgaag gttacggcga
      541 tgccgttttt cgccgcgatt tcggggtcga gcgtgtagtt gatgtatttg tgggcattgg
      601 cgacgttttt cgcatcggcg ggaatcagcc aagactcaat ccagaagccc atacctttcg
      661 gtgtcagcac ttcgatgccg acgttgtttt tcacttcttc ggaacgtgct ttcgccaagt
      721 tcaaatcgcc gccgttgcct gccgccaggc agatgtcgcc gcgcgccagc tcgtcaatga
      781 tggacgggct gaaacgtttg acatccggac ggatagactt caacacttcc gccgccgcct
      841 tcaaatcttc aggattcgat cctttgggat ctttgcccaa gtagttcagc aaaatcggga
      901 acatttcgct cggggtgtcc cacagggcga tgccgcagga tttcagtttg tgggtgtatt
      961 caggtttgaa cagcaaatcc cagccgtttt cgggcagctt gccgcccaaa agctctttgc
     1021 ctttcgccgt aatcgcaatc gtgttcacgc cggagaaata ggggacggca tactggttgc
     1081 ccgggtcggc ggtttccagc attttcaaga gttcgggatc gatgttttta tagttgggaa
     1141 tcaggtcttt gttgactttt tgatacgcgc ccgcctcgat ttggcgcggc aggaaggcga
     1201 tgcccggcac aaccaaatcg taaccggatt tgccggtcag cattttggct tccagcgttt
     1261 cattgttttc gtacaagtcg taagtgagct tcagattgtt ggctttttta aagtcttcga
     1321 ccgtactctc gtcaacatag ttcgaccagt tgtagatgtt cagagtatcg gtggcagcgg
     1381 cttcggcatt ggcaacagac gcagcgtctg cttgaggttg cacggcgttt ttttcgctgc
     1441 cgccgcaggc tgccagagac agcgcggcca aaacggctaa tacggatttt ttcatacggg
     1501 cagattcctg atgaaagagg ttggaaaaaa aagaaaaccc cgcgccccat aaacaccccg
     1561 gcgcaaggtt tgggcattgt aaagtaaatt tgtgcaaact caaagtgata ttggactgat
     1621 ttccctaaaa aattaccctg ttttcaaaag agggggaaaa cgtccgcccg attttgccgt
     1681 ttttttgcgc tgtcagggtg tccgacgggc ggatagagag aaaaggcttg catataatgt
     1741 aaaccccctt taaaattgcg cgtttacaga atttattttt cttctaggag attccaatat
     1801 ggcaaacagc gcacaagcac gcaaacgtgc ccgccagtcc gtcaaacaac gcgcccacaa
     1861 cgctagcctg cgtaccgcat tccgcactgc agtgaaaaaa gtattgaaag cagtcgaagc
     1921 aggcgataaa gctgccgcac aagcggttta ccaagagtcc gtcaaagtca tcgaccgcat
     1981 cgccgacaag ggcgtgttcc acaaaaacaa agcggcacgc cacaaaagcc gtctgtctgc
     2041 aaaagtaaaa gcactggctt gatttttgca aaaccgccaa ggcggttgat acgcgataag
     2101 cggaaaaccc tgaagcccga cggtttcggg gttttctgta ttgcgggggc aaaatcccga
     2161 aatggcggaa agggtgcggt ttttatccga atccgctata aaatgccgtt tgaaaaccaa
     2221 tatgccgaca atgggggcgg agatgaatac acggaatatg cgctatattc ttttgacagg
     2281 actgttgccg atggcatccg cttttggaga gaccgcgctg caatgcgccg ctttgacgga
     2341 caatgttacg cgtttggcgt gttacgacag gatttttgcg gcacagcttc cgtcttcggc
     2401 agggcaggaa gggcaggagt cgaaagccgt actcaatctg acggaaaccg tccgcagcag
     2461 cctggataag ggcgaggcgg tcattgttgt tgaaaaaggc ggggatgcgc ttcctgccga
     2521 cagtgcgggc gaaaccgccg acatctatac gcctttgagc ctgatgtacg acttggacaa
     2581 aaacgatttg cgcgggctgt tgggcgtacg cgaacacaat ccgatgtacc ttatgccgct
     2641 ctggtacaac aattcgccca actatgcccc gggttcgccg acgcgcggta cgactgtaca
     2701 ggaaaaattc ggacagcaga aacgtgcgga aaccaaattg caggtttcgt tcaaaagcaa
     2761 aattgccgaa gatttgttta aaacccgcgc ggatctgtgg ttcggctaca cccaaagatc
     2821 cgattggcag atttacaacc aaggcaggaa atccgcgccg ttccgcaata cggattacaa
     2881 acctgaaatt ttcctgaccc agcctgtgaa ggcagatttg ccgttcggcg gcaggctgcg
     2941 tatgctcggt gcgggttttg tccaccagtc caacggacag agccgtcccg aatcgcgttc
     3001 gtggaacagg atttacgcca tggcaggcat ggaatggggc aaattgacgg tgattccgcg
     3061 cgtgtgggtg cgtgcgttcg atcagagcgg cgataaaaac gacaatcccg atattgccga
     3121 ctatatgggg tatggcgacg tgaagctgca gtaccgcctg aacgacaggc agaatgtgta
     3181 ttccgtattg cgctacaacc ccaaaacggg ctacggcgcg attgaagccg cctacacgtt
     3241 tccgattaag ggcaaactca aaggcgtggt acgcggattc cacggttacg gcgagagcct
     3301 gatcgactac aaccacaagc agaacggtat cggtatcggg ttgatgttca acgacttgga
     3361 cggcatctga accgcgtgtt cagacggtat atcaagttgc cgtgccgtct gaagccgccg
     3421 gcggtttggc ggggcgggat aaaaatttcg tttgaatccg ggcaaacgcg tataatgtgc
     3481 ggtttgtacg gactttgtcg gaatcaagca agatgacgga acctgcggcc gaaggcggta
     3541 aagctgccaa ggcgttaaaa aaatatctga ttacgggcat tttggtctgg ctgccgattg
     3601 cggtaacggt ttgggtggtt tcctatatcg tttccgcgtc cgatcagctc gtcaacctgc
     3661 tgccgaagca atggcggccg caatatgttt tggggtttaa tatcccgggg ctgggcgtta
     3721 tcgttgccat tgccgtattg tttgtaaccg gattatttgc cgcaaacgta ttgggccggc
     3781 agattcttgc cgcgtgggac agcttgttgg ggcggattcc ggttgtgaag tccatctatt
     3841 cgagtgtgaa aaaagtatcc gaatcgttgc tgtccgacag cagccgttcg tttaaaacac
     3901 cagtactcgt gccgtttccc caatcgggta tttggacaat cgcattcgtg tccggtcagg
     3961 tgtcgaatgc ggttaaggcc gcattgccgc aggatggcga ttatctttcc gtgtatgttc
     4021 cgaccacgcc gaatccgacc ggcggttact atattatggt aaagaaaagc gatgtgcgcg
     4081 aactcgatat gagcgtggac gaagcattga aatatgtgat ttcgctgggt atggtcatcc
     4141 ctgacgacct gcccgtcaaa acattggcag gacctatgcc gtctgaaaag gcggatttgc
     4201 ccgaacaaca ataaagccgc cgttcagacg gcattttctg ttttcagttt aaatcaataa
     4261 aaggtgattt tatgcgtacc aactattgcg gtctgattag cgagcaatac ttagaccaaa
     4321 ccgttaccgt caaaggctgg gtacaccgtc gacgcgacca tggcggtgtg atttttatcg
     4381 acctgcgcga ccgcgaaggc atcgtccaag tcgtgatcga tcccgacacg cccgaagcgt
     4441 ttgccgctgc cgattcctcc cgcaacgaat acgttttgag cattaccggc cgcgtacgca
     4501 accgtcccga aggtacgacc aacgacaaaa tgatttccgg caaaatcgaa atccttgcca
     4561 aagaaatcga agtgttgaac gctgccgcta cgccgccgtt ccaaatcgac gacgaaaaca
     4621 tcagcgaaaa cgtgcgcctg accaaccgcg ttatcgactt gcgtcgtccg gtaatgcagc
     4681 gcaacctgcg cctgcgttac caagttgcta tgggcgttcg ccgctacttg gacgcgcaag
     4741 gtttcatcga cattgaaacc ccgatgctga cccgctccac gcccgaaggc gcgcgcgact
     4801 acctcgtgcc gagccgcgtt catccgggcg agttcttcgc gctgccgcaa tcgccgcaat
     4861 tgttcaaaca actgttgatg gtggcgggtt tcgaccgtta ctaccaaatc accaagtgct
     4921 tccgcgacga agacttgcgt gccgaccgtc agcctgaatt cacgcaaatc gacttggaaa
     4981 cctcgttctt aaacgaggat gaaatcatgg acatcaccga aggtatggcg aaacaagtct
     5041 tcaaagacgc gctgggcgtg gacttgggcg acttcccacg catgccttac tctgaagcca
     5101 tgttctacta cggctctgac aaaccggaca tgcgtatcaa cttgaaattc accgagttga
     5161 ccgacctgat gaaaacggaa gaattcaaag tcttccgtgg cgcagccgac atgaaaggcg
     5221 gccgcgtggt tgccctgcgt gtgccgaacg gtgcgaaatt cagccgcaaa gaaatcgacg
     5281 aatacactaa atttgtcggc atctacggtg cgaaaggtct ggcatacatc aaagtaaacg
     5341 atgtgggcaa cctttccaac ggcgaagaca gcggcctgca atccccgatt gtgaaattcc
     5401 tgtccgaaaa cgccctgaag gaaatcattg cgcgtaccgg cgcggaaaac ggcgacatca
     5461 tcttcttcgg tgcggacaaa gccaaagtcg tgaacgaagc catcggcgca ttgcgtatca
     5521 aagtcggctt ggaacacggc gcggaaaacg gctatttcac agacgaatgg aaacctttgt
     5581 gggttgtcga cttcccaatg tttgaatacg acgaagaagc cgaccgctac gttgccgtac
     5641 accatccgtt taccgcacca aaagaaggcc atgaagacct gatggtttcc gacccggcaa
     5701 attgtttggc gcgcgcctac gatatggtat tgaacggctg ggaaatcggc ggcggctcta
     5761 tccgtatcca ccgcgcagac gtgcaagaga aagtgtttgc cgcgctgaaa atcagccctg
     5821 aagagcaaca agagaaattc ggcttcctct tggacaacct gaaattcggt gcgcctcctc
     5881 acggcggcct tgcattcggc ctcgaccgtc tggtaacgct gatgaccgga gccgaatcca
     5941 tccgcgacgt gattgccttc ccgaaaacac aacgcgcaca atgcctgctg accaacgcgc
     6001 ccaacagcgt ggacgacaag cagttgcgtg aattgagcct gcgcctgcgc caaaaggtgg
     6061 ctgaaaccaa agaagcataa ggaaaacaga gccgtttgac ggctctgttt ttttcagacg
     6121 gcatttacgc ttcttgactt ccctctaatt caaacctaat tttgctgtgt tttaatggcg
     6181 gtattgaaaa acacatcttg ttcaatcaac cgataaaaaa aggactgaaa atgaaaaaac
     6241 tgttattggc cgccgttgtt tccctgagtg ccgcaaccgc atttgccggc gattctgccg
     6301 agcgtcagat ttacggcgat ccccattttg aacaaaaccg cacaaaagcc gtgaaaatgt
     6361 tggaacagcg tggttatcag gttcacgatg tcgatgccga cgaccattgg ggtaagcctg
     6421 tgctggaagt ggaagcctat aaagacggcc gcgaatacga catcgtgttg tcttaccccg
     6481 acctgaaaat catcaaagag cagctcgatc gctgactcct ttgatggcaa gctgaaccaa
     6541 aatgccgtct gaagcgttca gacggcattt tgcctgttcc tcatcaggta tgaggcaggc
     6601 ttttcttatt aaaaaaatga catttcacgc tgatttgtta taatcattcc ttttcaacac
     6661 gacagacgga gcaggtttat tatgcctatc cttaccatcc gtgaagtgtg caacattaat
     6721 cattggggca taggttatta tgatgttgac gattccggcg aaatcatcgt ccgccccaat
     6781 ccggcgcaac acaatcaaac tgcttcactg caaaaactga ctgaagccgt gcaacaaaaa
     6841 catcaggcgc gcctgcctgt tttgttttgt tttccgcaaa tcctcgaaca ccgcctccgc
     6901 gacattaacc gcgcctttca gacggcacgg gaagagtgcg gctataaggg cggttattgt
     6961 ttggtttacc ctatcaaggt caaccaacac cgccgcgtca tcgaatcgct tatgtcaagc
     7021 ggacaaccgc atggtttgga agctggttct aaagccgaac tgatggcggt tttggcacac
     7081 gccggcaccc ggcaaacatt aatcgtctgc aacggctata aagaccgtga atatatccgt
     7141 ttcgccttga tgggcgaaaa actggggcat caggtttatt tggtgattga gaagctgtcc
     7201 gaaatacaaa tggtattgga agaggcggaa aaactcggca tcaagccccg tttgggtgtg
     7261 cgcgccagac tggcttccca aggttcggga aaatggcagt cttcgggtgg ggaaaaatca
     7321 aaattcggct tgtcggcttc ccaagttttg caactggtcg atattttgaa acaaaaaaac
     7381 aggctggatt gcctgcagct tttgcatttc catttgggct cgcagcttgg gaacatccgt
     7441 gatgttgcca caggtgtaca cgaatcggct cggttttatg ttgagttgca caaactgggg
     7501 gtaaatatcc gctgttttga tgtaggcggc gggcttggcg tggattacga aggaaaccgc
     7561 acacaatcgg attgttccgt taattacagc ctcaacgaat atgccgccac agtcgtatgg
     7621 ggcatcagtc aggcttgtct cgaacacggg ctgccgcatc cgacaatcat caccgagagc
     7681 gggcgcggca ttaccgcaca tcacgccgtt ttggttgcta atgttatagg cgttgagcgt
     7741 tacaaaccgc gccggctgga tgcgccatcg cccgaagcgc cgcgtgtgtt gcacagtatg
     7801 tgggaaactt ggacggatat ttccgcctcg cgggaaaaac gttccttacg cagctggata
     7861 cacgaagggc agtttgatct tgccgatgtg cataatcagt ataatgtcgg gctgttgagt
     7921 ttggcgcaac gtgcgtgggc ggagcaactg tatttaaata tctgtcatga agtcggcgaa
     7981 ttgtttaatg aaaaacaccg gtctcaccga accattattg acgaattgca agaacgtttt
     8041 gccgataagc tgtatgtcaa tttctcactc ttccaatctt tgcccgatgc ttggggcata
     8101 gatcaacttt tccctgtttg tcccattacc ggtttgaatg aaccgattgc gcgccgcgcc
     8161 gtgttgttgg acattacctg cgattcagac ggtacgattg accactacat cgacggcgac
     8221 ggcatcgccg gtacgatgcc tatgcctgat tatcccgaag aagagccgcc gcttttaggc
     8281 ttttttatgg tgggagcata tcaggaaata ctcggcaata tgcacaatct tttcggcgac
     8341 actgccactg ccgatgttgt tgtaggggaa gacggacaat ttaccgtcat cgattacgat
     8401 gaaggaaaca ccgttgccga tatgctcgaa tacgtttatc aagatccgaa agagctgatg
     8461 aaacgctatc gcgaacaaat cgaacattca gaccttcctg cctcgcaggc tatgtctttc
     8521 ttaaaagaac tcgaagcggg gcttaatggt tatacctatt tggaagacga atagacgcat
     8581 caaggcatcg gatatgccgt ctgaagcccg attttcttcc tcaaacacca atcaccacga
     8641 ccgattgaaa ccaattacaa ggaatcatta cgatgcaata cagcacactg gcaggacaaa
     8701 ccgacaactc cctcgtttcc aataatttcg ggtttttgcg cctgccgctt aattttatgc
     8761 cgtatgaaag ccaagcagat tgggtcatta ccggcgtgcc ttatgatatg gcggtttcag
     8821 ggcgttccgg cgcgcgtttc ggtcctgaag ccatccggcg cgcctccgtc aacctcgctt
     8881 gggagcaccg caggtttcca tggacatttg atgtgcgcga acgcctgaac attattgatt
     8941 gcggcgactt ggttttttct tttggcgaca gcagggattt tgtcgaaaaa atggaagcgc
     9001 acgccggcaa attactttct tccggcaaac gctgtttgag tttgggcggc gaccatttca
     9061 ttaccctccc gttgttgcgc gcccacgccc gctatttcgg caaactcgca ctgattcatt
     9121 ttgacgcgca caccgacacc tacgacaacg gcagcgaata cgaccacggt acgatgttct
     9181 ataccgcccc caaggaaggc ctcatcgacc cgtcccgttc cgtacaaatc ggcatacgca
     9241 ccgaacacag taaaaaattg ccttttactg tgttgtccgc ccctaaagtc aatgaagaca
     9301 gtgttgaaga gaccgtccgt aaaatcaaag aaaccgtcgg caatatgccc gtttacctga
     9361 ctttcgacat cgactgcctg gacccgtcgt tcgcccctgg gaccggtacg cccgtatgcg
     9421 gcggcttgag cagcgacagg gcattaaaaa tcctacgtgg gctgacggat ctcgacatcg
     9481 tcggtatgga tgttgtagaa gttgccccct cttacgacca atccgacatt accgctttgg
     9541 ccggcgccac aattgccttg gaaatgcttt accttcaagg tgcgaaaaag gactgaacgt
     9601 ccggcatccc ccgggttttc gccgtgccgt tcaaacggcg tattcagtct aatgaaaatt
     9661 caaatactga aacaaaagtt gcccggagcc gcatatcgga aagacggtga aatatcggaa
     9721 tatatcttat aaaacaatta gttaaatatt atttttccga tttttcggga cggtcttttt
     9781 tacggaggtc aatatgatga aattgggttt caaaccgata cccctcgcca ttgccgcagt
     9841 attgtgcgcc ctggttttgg cactgcccgt acccgacggg gtcaagcctc aggcttggac
     9901 gctgctggcc atgtttgtcg gtgtgattgc cgccattatc ggcaaggcca tgccgttggg
     9961 cgcgctgtcg attattgccg tcgggttggt cgcagtaacc ggcgtaaccg ccgacaaacc
    10021 gggcgcggcg atgagcgatg cgttgagtgc gttcgccaat ccgttgattt ggctgattgc
    10081 catcgcagtt atgatttcgc gcggtttgct caaaacaggg ctggggatgc gtatcggata
    10141 tttgtttatc gccgtttttg gaagaaaaac gctgggcatc ggttacagtc tcgctctttc
    10201 cgaactgctg ctggctcccg ttaccccttc caataccgcg cgcggcggcg gcattataca
    10261 tccgattatg cagtcgattg ccggcagtta cggctccaat cccgcaaaag gcacagaagg
    10321 caagatgggt aaatatttgg ctttggtcaa ctatcattcc aatcccattt cgtcggctat
    10381 gtttattact gcaactgccc ccaacccttt aatcgtcaac ttgattgccg aaaatttagg
    10441 cagtagtttc cgtctttctt ggggggcgtg ggcgtgggca atggctgttc ccggcgttat
    10501 cgcctttttc gttatgcctt tgattttata ttttttgtat ccgcctgaaa ttaaagaaac
    10561 gcccaatgcc gttcaatttg ccaaagaccg tctgagggag atgggtaaaa tgtcggcaga
    10621 cgaaatcatt atggcggtca ttttcggtat cttgttgctg ttgtgggcag atgttcccgc
    10681 ccttattacc ggcaatcacg cttttagtat caacgccacc gccaccgcat ttatcggatt
    10741 aagcctgctt ttgctttccg gtgtattgac ttgggacgat gttttgaaag aaaaaagtgc
    10801 gtgggatacg attatttggt ttggcgcatt gattatgatg gccgcatttt taaataaact
    10861 cggactgatt aaatggttct ccggagtgtt ggcggaaagt gtcggcggtt tgggcgttag
    10921 cggcacggct gcgggcgtaa tcctcgtgct tgcttatatg tatgcgcatt atatgtttgc
    10981 cagtactact gcacatatta ccgctatgtt cggcgcattt ctcgctgctg ccgtttcact
    11041 gaatgcccct gcgatgccga ccgcgctgat gatggcggcc gcatccaaca ttatgatgac
    11101 cctcactcat tatgcgaccg gtacttcgcc tgtgattttc ggttcgggct acaccacaat
    11161 gggagaatgg tggaaggcgg gttttatcat gagcgtagtc aattttctga tttttttcgt
    11221 tatcggcagc atttggtgga aagttctggg gtattggtaa gggaaaaata aaataaattt
    11281 ccaatctgtg tttatttgat tgggcgacta ttatcgtgaa atatgccgtc taaagccttc
    11341 agatggcata tttgtgcgct tgaatgttgc agaaagcggc aggcggcggt gtaggaaaag
    11401 ccaaacaaaa accaaaccgc ctatcaactt ctgataaaca taagcattaa ataatcagaa
    11461 ggttattcaa ttacctaaac gcaaatttcc cttacctaaa cgcaaatttc cctgccgtat
    11521 cacatctatt gaaaataata catcaaccgg ctcggaagca gcctgatcag gtgtttctac
    11581 ttgcggcgat gaatcggcag ccggttcggt ataggcagtc ggcgtgccgt cggattgggt
    11641 atttaaatgc ggttgtgcct cgggcgtgtg tacatcaggc acttcgggcg tgcgtgtctc
    11701 ggatatttcg gcagagttgg tttcctcagt ttgttcaatg acttcagctt ggctgtatga
    11761 ggaagaaccc tgtatccacg ccagcgattt gagcggcatc ttcatcttgc cgtttttgcc
    11821 gcaggtcagg cagacggccg atccggtgcg gtctttgagt acagcatcga ctttctcggg
    11881 cgcgatggtt ttaccctgtg tttttgccca ttgcgcaata ctttttttca gagaggcgat
    11941 gtcaaggttg ttcttaccgt aagggtcgcg gaaggcggca agccacaggt tgcgatccgc
    12001 atcttcattg agacccgcca tttgatagat gacggaggcg ggcgagccgc tggcgatggt
    12061 tttggcaaac tcgagcgcgt cgggcgattt catgcggacg cagccgtgac tccgaacccc
    12121 ggggacgctg gccggcgcat tggtcccgtg tatgcccaaa ccgagtttgg ggtcgcctaa
    12181 gcggacaaaa accggcccca aagggttgtc cgggccggcg gctatggttt ttacgccgtc
    12241 gccgcgttct ttctgtatgg atttggggat gtaccaaaca gggttatagg ctttcgcccc
    12301 gattttatgt tcgcctagat tggtttgcgt catcgcccga cctaccgcaa cgggataaac
    12361 cttggtcagt ttgccgtcgg tgtagaggaa caggcgttgc tgagggatgt taatgaagac
    12421 atgttgacct tgtgcgacgg gggagacatc gggaatgacg gtgtttgcgt atgaaaaacc
    12481 gcttatcaat agtgcagcag tgcggcagat tgttttattc atatcaaaat atggtgtgtg
    12541 tccgataggt tttcggcaaa tcatacctga aaccgtacca atttgtgcga aaatatgcgc
    12601 ttcggtacag tgcggacgga ttgagtgatg gcaacggaaa caaatgtcgc ggaaatttcc
    12661 gccttggatt atgaaggcag gggtgtggca aaggtcggcg gcaaaacggt ttttattaaa
    12721 agggcattac ttgattgttt gatgctgggt tggttcaggc tttaactcag gaatatttac
    12781 atcataatga aggtttttaa acaacagctt gaacaactcg gcgcgcaaaa ccaatatcgt
    12841 tcgattccgg atttgattca tcaagggcgg tatattacgc gggaaaactg caaaatgctg
    12901 aatatgtcgt ctaatgatta tttgggtttg gcatcagatg aaaacttgcg ccggtctttt
    12961 ttgcagcaat acggcggtaa ttttccctct tttaccagtt cttcatcgcg tttattaacg
    13021 ggcaactttc ctatttatac cgatttggaa gagcttgtcg cacaacgttt ccaacgggaa
    13081 agcgcgttat tgttcaacag cggctatcac gccaatctcg gtattttgcc tgctttgacg
    13141 acgacgaaaa gtttgatttt ggcagataaa tttgttcacg ccagtatgat tgacggcatc
    13201 cgtttgagcc ggtgtgcgtt tttccgttat cgtcataatg attatgaaca tttgaaaaat
    13261 ctgcttgaaa aaaacgtcgg aaaatttgac cgcactttta tcgttaccga atctgttttc
    13321 agtatggacg gtgatgtggc ggatttgaaa cagcttgtcc aattaaaaaa acagtttccc
    13381 aatatttatc tttatgtgga tgaagcccac gcaatcggtg tttatgggca aaacggattg
    13441 gggattgccg aacgggataa tttgattgcc gagattgatt tattggtcgg cactttcggt
    13501 aaagccttag cctcggtggg ggcgtatgcc gtctgcaacc aagtattgaa agaatgtttg
    13561 attaatcaaa tgcgcccatt gattttttca accgcattgc cgccgtttaa tgtggcttgg
    13621 acttatttta tttttgaacg attgccgcaa ttctcaaaag aaagaagcca tcttgagcag
    13681 ttaagcgcat ttttacggca ggaagtggcg catcggacgc aaataatgcc gagccaaacc
    13741 tgtatcgtcc cctatatttt aggcgggaat gaagccgccc ttgccaaagc ggaatacctg
    13801 caaaggcagg gttattattg cctgcccatc agaccgccga cagtacccaa aaacacatcc
    13861 agaatccgcc tgtctttaac ggcagatatg acaacggatg aagtgcggca gtttgcggcg
    13921 tgcctgtaag gatatgatat gaaaacaaaa ttttacgact gccaaggcga acatttaatt
    13981 ctgtattttg caggttgggg aacgccgccc gatgctgtaa atcatttgat tttgccgaca
    14041 aatcacgatt tattgatttg ctatgattac caagatttac gtttggattt tgatttttcc
    14101 tcctatcggc acatccgttt ggtggcgtgg tcaatgggcg tttgggcggc agagagggca
    14161 ttgcaaggaa taagactaaa atccgcaacg gcagtgaatg gcacaggttt gccttgcgat
    14221 gataatttcg gtatcccttg cgccgttttt aaaggcacat tggagaacct cacggaaaat
    14281 acccgtttaa aatttgaacg cagaatgtgt ggcgataaag catcttttga agattaccaa
    14341 caatttcccg cacgcccgtt tgacgaaatt catcaagaac ttaccgcact ttttgcgatg
    14401 atcgggcaag ataggcgtac agatcttatc tgctggacaa atgccttggt cggatcgggc
    14461 gataaaattt ttatgcctgc caatcagcac cgatattgga caccgcgttg caccgttcgg
    14521 gaaattgacg gcggacatta cctgttttca agattcaccc attggtcggc actatggaat
    14581 cactgactgc cataaataaa tcgcgcattc ggcaggcttt ccaaaaagca ttaaacgatt
    14641 atgaccggca cgccttaatc caacaaaaaa tgacgattaa tttaatgacg catttgcaag
    14701 attatttgcc ggatatgcca ttggaaaacg tgttggaatt gggctgcggc tcaggaatgt
    14761 tgagtgcctt gctgcaaaaa cagatttcag cgaattattg gttatttaat gatttgtgca
    14821 atgtgcagcc ccaactggct gaaaaactgc cgcaatcctt tgatttttat tgcggcgatg
    14881 cggaaaactt tccttttcaa cgacaatttg acttaatcgc aagcgcatct gccgtgcaat
    14941 ggtttcatca acccgacgct tttatcaccc attgcaaaac aggcttgaaa acaaacggat
    15001 tattggcggt tgcaaccttt ggcaaagaca atttaaaaga agtccgccaa attacaaata
    15061 taggcttaaa ttacccgact ttatcccaat ggcaggcttg gttagccaaa gattttgagc
    15121 ttttatggtg tgaggatttt acggtaatac tagactttga tacgccgtca gatgtactca
    15181 aacaccttaa atatacaggc gtaacagcca cgaaccaaaa aaattggaca agaaaaaatc
    15241 tcaatggatt tattggcgat tacttgtcgg cgttcggtat gccgtcgggc aaagtgcgcc
    15301 tgacttatca tccgttattt tttatcgcgc gctactctgc ggcgggcagg caataaggca
    15361 gcttatgggc aaagttattt ttatatcggg tattgatact gatgtgggta aaaggtaata
    15421 tggcgaggct tgtgcagaag gcatattgtt aaacgttaaa ttatggtatg atttaaaact
    15481 tacaagtcta tttcagtaaa tcgttaataa taaaagcgga caatggccgt tgcaggcggt
    15541 cagctatccg ctcgttacgg tatcgggagt atcctttgga aaactcatta attatcggtt
    15601 tggcactggc ggtattgctg atgcttttgg ttatgcgtgc gaagcagggt aagaaaacac
    15661 cgaagcgcaa atcccaaggt gtcggcaata cgcaggggca gacacccgga agcaatgatt
    15721 cggactgggt cgatcagatt ggacaatcgg tttcagacgg cacgcaaccc gactggtctt
    15781 ggaacgaaag tgccgagacc gcatccgccg ccgtatccgc gcaagaagtc gatccgctta
    15841 cggagtatca ggtttataag caattcggtt atcagggcaa ggctgccgaa tctttggctg
    15901 cctatctgga cggcattccg gatggtgaag cgaaacctga aaaccttatc cgcgagctgc
    15961 tcgatatcaa tctcgaagtg ggggatgtcg atgttttggc agacaatctg caaaaatacg
    16021 gcaaactgat tccttccgaa cttttggcaa aatatatcga acaggcatta cagcgcgatt
    16081 caaaccattt gcgtatccgc gtcttggcgg aagaaggttt gggatggggt actcaggaga
    16141 ttgaaaaacg tgcggaaggc ggttctgcga cggcagcttc cgcatcgccc ccgccggatg
    16201 ccggcggtaa ggcttatgaa gccgaagaaa tcaagcgcat cccgattgtg cggggcaaaa
    16261 aagacgtgtc cggaatcagt caagaggaaa tcggtgcgat tgccggtttg gtccgtgccg
    16321 atcaaggtgc gaaaatcctt aaagacaaag tcagctatga aacggcatcg aaacaatacg
    16381 accgtgccat ccaaacttcc gaaaaacctg caaacctgat tatcgatgcg ttgaaactcg
    16441 attaccaata cgcggacata gaccgttttg ccggacattt gtggaaactt taccaaacgt
    16501 tgggcaacta cggcaggcag gttaaagagc ggatgctggg gtgggggtac agcctgggtt
    16561 accatgaggt tttcgatgat ttggaaaaag ggccgaacga ccggcaaatc aaagacatcg
    16621 gtatggggca cgggtatctg ccgaaaaata tacagaaatt caaatcgcaa catcgggatt
    16681 tggtgcttca agattcttcg ttgatcaaca ccggttcgtc tccggcagac gatgcggtta
    16741 aggaagtaga gtcgttgctg atgtatggtc agattgaagc ggcaatggat gtgttggagc
    16801 aggcggtatt gaaatatccc gacgagtccc agctttatat tacgttgatc gatatttatg
    16861 aacgtactga agattgggat aggttggggc agtttttaag ggtattgagg gaacgtgcgg
    16921 acaggcttcc tgaagaggtc gttatgctga tgagccggct gctgcagcgt atgaatcaaa
    16981 atattaaaaa aataaaacgg tacggaaaat aaaaatggaa gttcaactgc cgaaaattaa
    17041 aacagtacgc gtaatgttgg cggggatgac ggcgcagcag gaatccgttt tcaaaatggc
    17101 attcaaaatg cacaatacca cccgttatga aacagtatcc ccttcagacg gcagtgccgt
    17161 gcccgatttg gttttggcgg ataccgatgc cgagggcggt tttgaacttt ggaaagagct
    17221 tgccgagcgt tataaggata tacccgtcgc cgtctgttcg gagaaagttc ccgattctga
    17281 agttccctac ctgcccaaac cgattcggtt tgaaacattg tttcctatgc tccgcaaatt
    17341 gttgcagggc gagaatgttt atgggaaatc gtttattgca cccgcagacc ggtcggcgaa
    17401 aaataacggg aatgtgcagc gtacggttac gatacgccag tttaacccga ataaaggatt
    17461 attgggggcg ttgcggtttg cggaaaagaa caggcaggac atcgctatct tgcatggaaa
    17521 taagccggtc cttattgttt tcccctcgat acaacgggtt ttgctgacag aaagtgtgca
    17581 aaaactcgaa gaattgtgca aagacgaaaa tttgcaggtc agctgcaaga ctgttcccga
    17641 taacccgcaa tggcgcgaaa aggctaaagt aggcattatg tcctgtatgt ggcagttttc
    17701 catttggacg gcgcagggca ggttgattta tccgatttct cccgatactc cgtttacgtt
    17761 gaaatcttgg ccaaacctga cccggttggc aaatgtgccg gggtcgatac gcttgtcggc
    17821 atttctgacc aaggcatccg tcaaccttaa cgtgttgtat aaagtgatgc ctttaaacct
    17881 caatgatatt ctgaattatc ttgcggcaac ctatacaacc gggtttttgt cggtagattt
    17941 aaaaacggtt tcacaacagg catactccga tatggcggat aaaataaata tcggagccga
    18001 ttctgcctct gatagtgaaa tgatgaaaaa agcggaaaaa atcacaacac catcccaatc
    18061 ccagtcgcgc ggtcttctgc aaaggctgat gaaaaaactg ttgggcagct aagaggcgga
    18121 gagatgagag aaaataaaat tattttcaca ggacctgtcg gcgtagggaa aaccactgcc
    18181 attgcggcta tttcggacga agcactcgtt cagaccgatg cttccgcatc cgatatgact
    18241 ttggatagga aaaggaatac gacagtggcg atggactacg gggccatcag cttggatgag
    18301 gataccaaag tccatttata tggtacgccc ggtcaggaac ggttcaactt tatgtgggaa
    18361 atcttaagcc aaggcagtat gggtttggtc ttgcttttag ataatgcccg aaccaatccg
    18421 ttgaaagatt tggaattctt tttacattcg tttcgagggc tgctggagaa ggcacccgtc
    18481 gttgtcggta ttaccaagat ggatatacgc tctcagcccg gtatcgacgt gtatcacaaa
    18541 tatcttgcaa aacataatct taatgttccg gtttttgaaa ttgatgcccg taaggaagat
    18601 gacgtaaaac aattggttag cgcaatgtta ttttctattg atccgggact ggaggtttaa
    18661 tatggaatca acactttcac tacaagcaaa tttatatccc cgcctgactc ctgccggtgc
    18721 attttatgcc gtatccagcg atgcccccag tgccggtaaa actttgttgc acagcctgtt
    18781 gaaagcggat gcggacgaaa tggtcagcag tgagaagctg cttacttggg cggacaccgc
    18841 cgacatcgat accgctttga acctgttgta ccgtttgcaa aaactcgaat tcctctatgg
    18901 cgatgaaaac ggtcattcag acggcatcaa tttgtcggac gagcaattgc cgttgctgat
    18961 ggaacaattg tccggcagcg gtaaggcgtt attggtcgat cggaacggtc tgtatcttgc
    19021 caacgccaat ttccatcatg aggcggcgga agagttgggg ttgttggcgg cagaagtcgc
    19081 acagatggaa aagaaatacc ggctgctgat taagaacaac ctgtatatca acaataacgc
    19141 ttggggcgtt tgcgatcctt ccggtcagag cgaattgaca tttttcccat tgtatatcgg
    19201 ttcaaccaaa tttattttgg ttatcggcgg cattcccgat ttgggcaaag aggcatttgt
    19261 tactttggta aggattttat accgccgtta cagcaaccgc gtgtaaaact tgggagagag
    19321 gaggggttat gcagcaatta ttgatttcaa tccttgaaga tttaaacaat acatctacgg
    19381 atattatcgc gtctgccgtt atctcaaccg acggattgcc gatggcgaca atgcttcctt
    19441 cacatttgaa ttcggacagg gtaggggcga tttctgccac tttgcttgct ttggggagtc
    19501 gctcggtgca ggaactcgcc tgcggggaat tggaacaagt gatgattaaa ggaaaatcag
    19561 gctatatcct tttaagtcag gcgggtaaag atgccgtgtt ggtgctggtg gcaaaagaaa
    19621 ccggcagact tggtttaatc ctattggatg ccaaacgtgc ggcaaggcat attgcggaag
    19681 ccatataaca tataaagatt gcgggcttgc agataaagtg caatcgattg tcaatttata
    19741 ttgacacgtt cggtatttct gttttattat tcgcgcttat tccccgatag ctcagtcggt
    19801 agagcgacgg actgttaatc cgcaggtccc tggttcgagc ccaggtcggg gagccaaatt
    19861 tcaaaaccct ctaagtattt tcttagaggg ttttgtttta ccggcggtca gaaacgcatt
    19921 tttgagatga ttgttttgag atggaataaa atctttgcaa aattcctttc gtgatggtta
    19981 tgaaaaaata ggggctgtcc tggacaacta ggataaactc gattttatag tggattaaca
    20041 aaaaccagta cggcattggc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
    20101 gcaccaagtg aatcggtttc gtactatttg tactgtctgc ggcttcgtcg tcttgtcctg
    20161 atttttgtta atccactata ctaattgaga cctttgcaaa attcctttcc ctcccgacag
    20221 ccgaaatccc aacacaggtt ttcggctgtt ttcgccccaa acacctccta attctaccca
    20281 aataccccct taatcctccc cggatacccg ataatcaggc atccgggtta ccttttaggc
    20341 ggcaacaggc gcacttagcc tgagaccttt gcaaatttgt cggtttcggg gtcgtattgg
    20401 tagcctcgtg cctgtatgtc ttctttgaaa gtttcgtata cgtcatgggc taaaagggct
    20461 gttccgacat agggaaccgc ccttgtgctg aatttcgcgc ctaagcgggc aagtttgccg
    20521 ccccccgcca atacgccggc gcgggaaatg ctggcggtta ttttggcgtt gattcgggct
    20581 tttgcgcccg tagggatgtg tgttaaatct accgttttta ttaaatcaga tgaataagtt
    20641 ttactat
//
LOCUS       DAWFKN010000029        20337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFKN010000029 DAWFKN010000000
VERSION     DAWFKN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.029"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGR46_001068"
     CDS             complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGR46_001068"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0856021.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDTEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGR46_001069"
     CDS             complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGR46_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0856022.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRIFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDDIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAR"
     gene            complement(3572..4999)
                     /locus_tag="WGR46_001070"
     CDS             complement(3572..4999)
                     /locus_tag="WGR46_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0856023.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5159..6148
                     /gene="pdxA"
                     /locus_tag="WGR46_001071"
     CDS             5159..6148
                     /gene="pdxA"
                     /locus_tag="WGR46_001071"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0856024.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPTAEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKDLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGHL
                     GHEETDTIIPALKNLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(7032..9791)
                     /locus_tag="WGR46_001072"
     CDS             complement(7032..9791)
                     /locus_tag="WGR46_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0856025.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENSKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTSGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            10285..11277
                     /locus_tag="WGR46_001073"
     CDS             10285..11277
                     /locus_tag="WGR46_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0856026.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPAREFDIVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11325..12497
                     /gene="lpxB"
                     /locus_tag="WGR46_001074"
     CDS             11325..12497
                     /gene="lpxB"
                     /locus_tag="WGR46_001074"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0856027.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            12505..12751
                     /locus_tag="WGR46_001075"
                     /pseudo
     CDS             12505..12751
                     /locus_tag="WGR46_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12753..13163
                     /locus_tag="WGR46_001076"
     CDS             12753..13163
                     /locus_tag="WGR46_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856028.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQESFN"
     gene            complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGR46_001077"
     CDS             complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGR46_001077"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0856029.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(14096..14473)
                     /locus_tag="WGR46_001078"
     CDS             complement(14096..14473)
                     /locus_tag="WGR46_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0856030.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            14692..15126
                     /gene="fur"
                     /locus_tag="WGR46_001079"
     CDS             14692..15126
                     /gene="fur"
                     /locus_tag="WGR46_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0856031.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            15196..15921
                     /locus_tag="WGR46_001080"
     CDS             15196..15921
                     /locus_tag="WGR46_001080"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0856032.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGR46_001081"
     CDS             complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGR46_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0856033.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(17305..18144)
                     /locus_tag="WGR46_001082"
     CDS             complement(17305..18144)
                     /locus_tag="WGR46_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0856034.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18293..20269
                     /locus_tag="WGR46_001083"
     CDS             18293..20269
                     /locus_tag="WGR46_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0856035.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVAKNA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
BASE COUNT         4006 a         4906 c         6359 g         5066 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaaaacggaa
      541 accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt ttttttttga
      601 ttttctattt ttcgcaggaa tgacggtttg ggttctttct ctttgaagtt gcgatgctga
      661 aaatgccgtc tgaagttcag acggcatttt tgtgccggtt taaaacaagg cctgctgcgc
      721 gagcaggttt ctgacggggg cgaagtctcg gcggtgttcg ggcagcacgc cgtattgttt
      781 gagggcttcc agatgctgct tcgtgccgta acctttgtgt ttgtcgaaac cgtattgggg
      841 atggcgttgc gccagtgcgt acatttctgt atcgcgtgcg gtctttgcca aaacggatgc
      901 ggcggagatt tcgatgattt tgctgtcgcc tttgacgacg gcttcggcag ggatgttcaa
      961 atgttcggga atgcggttgc cgtcgatgaa tattctttcg ggacgcacag ccaaaccgtc
     1021 aacggcgcgt ttcatcgcga gcatggtggc gtgcaggatg ttgaggctgg cgatttcttc
     1081 gggcgtggcg gcggcgacgt gccacgcggc cgcctgattt tttatcattt cggcaagcgc
     1141 gtcgcgtttt ttctcgctga gttttttgga gtcggtcagt ccgggcaggt cgaatgtttc
     1201 cggaaggatg acggcggcgg caaacacgct gccgactaaa ggtccgcgtc ctgcctcgtc
     1261 cacgccggcg gtcagtatgt gcatgatgtt tcctgtcggg atggtacgat gccgtctgaa
     1321 aagggtttca gacggcatcg cgccgatgtg tttatctcgc gtctttaaat ccgcgcttca
     1381 aatgcaccat cagcaatgcc actgccgcag gggttacgcc ggaaatgcgg cttgcttgtc
     1441 cgacggtttc gggtttgtgc tgattgagct tttgctgcac ttctgccgac aagcctttga
     1501 ctttgccgta atcgatatcg tcgggcagct ttaaggtttc gatgtcgcgg cggctgtcga
     1561 tttcttcgtt ttggcggtcg atatagcctt ggtatttgac ttggatttcg acttgttcga
     1621 tgacttcggc ggagaggttt tcagacggca tcgcgccttc gagcgtcatc agcgcggcgt
     1681 agtcgaggtt cgggcggcgt aacaaatcgt gcaggttggc ctcgcggctg agtttttgtc
     1741 cgaacacgcg gatttgttcg tcttcggcga gtttttgcgg tgtgtaccac gttgttttca
     1801 agcgttggat ttcgcgttcg acggcttcgc gtttttcgtt gaaaatgcgc cattgcgctt
     1861 cacccaccaa gccgattttg tagccgtctt cggtcaggcg catgtcggcg ttgtcttccc
     1921 tgagttgcag gcggtattcg gcgcggctgg tgaacatccg gtaaggttcg ttcacgcctt
     1981 tggtgatgag gtcgtccacc aatacgccga gataggcttg ttcgcggcgc agcaggagcg
     2041 gatcttgtcc gcggacgtat tgcacggcgt tcgcgcctgc gagcaggcct tgcgcggcgg
     2101 cttcttcgta gcctgtcgtg ccgttgattt gcccggcgaa aaacagtccc tcaatggttt
     2161 tggtttcgag gcttgctttg aggttgcgcg gatcgaagta gtcgtattcg atggcgtagc
     2221 cggggcgcag gatatgggcg ttttccaggc ctttcatgct gcgaacgagc gcgatttgaa
     2281 tgtcgaaggg caggctggtg gagataccgt tggggtagta ttcgtgcgtg gtcaggcctt
     2341 cgggctcgag gaaaatctgg tggctgtctt tatcggcgaa gcggttgatt ttgtcttcga
     2401 tagacggaca ataacgcggg cccacgcctt cgattttgcc ggtgaacatc gggctgcggt
     2461 cgaagcctga gcggatgatg tcgtgggttt gcgtgttggt atgcgtaatc cagcaggaca
     2521 cttggcgcgg gtgcatttcg gcgttgccgc gcacggacat gacgggaacg ggtgtgtcgc
     2581 cgggctgttc ggtcagttgg gaaaagtcaa tcgtgcgtcc gtcgatgcgc ggcggcgtgc
     2641 cggttttcag acggccttgc ggcagcttca attcgcgcaa acgtccgccc agcgatttgg
     2701 cggcggggtc gccggcgcgt ccgccctcgt agttttccaa accgatgtgg attttgccgg
     2761 acaaaaacgt gcctgcggtc aacaccacgg cgcgtgcttt aaactccacg cccatcgcgg
     2821 tgattacgcc tgaaatgcgg tcgccatcga gcgttacgtc ttcgacggct tgttggaaaa
     2881 ggtcgaggtt ttcttggttt tccaacattt cgcggatggc ggctttatat aggatacgat
     2941 ccgcctgcgc gcgcgtggcc cgtactgccg cgcctttgct ggcgttcaga cggcggaact
     3001 ggatgccgga tttgtcggtt gccaacgcca tcgcgccgcc gagcgcgtcg agttcgcgca
     3061 ccaaatgccc tttgccgatg ccgccgatgg aagggttgca cgacatttgt ccgagcgttt
     3121 caatattgtg tgtgagcaaa agcgtctgcg cgcccatacg ggcggcggcg agcgcggctt
     3181 ctgtgccggc gtgtccgccg ccgacgacga taacgtcgta ggttttgggg taaatcatgt
     3241 gggtcatgtg tgtattgcct gtcggtgttt cagacggcat ttatagtgga ttaacaaaaa
     3301 ccagtacagc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagtac
     3361 caagtgaatc ggtttcgtac tgcttgtact gtctgcggct tcgtcgcctt gtcctgattt
     3421 ttgttaaacc actatattca atatgccgtc tgaaaaacga aatggattca aaagtaaagg
     3481 gttgggattg tacgcttgtt cgccctgttt ttacagtgtg cggaaaggga aaagccgctt
     3541 cgcggggaag cggctccggt aagggcggga tttaccaaac gtcggatttg atacggcgtt
     3601 tcaggcccgg atgttcggaa agtttgaact cggggtcttt gcccattttc agcttggcgg
     3661 tgtaatcgcg cagcagcata aacgccaagg gcgagagcag caggatggcg acaaggttga
     3721 tccacgccat aatgcccatc gccatatccg ccatatccca gaccaaaggc acattggcaa
     3781 ccgcgccgaa atagacccac gccaaaacca gcatacggaa aacggcggta atcagccaat
     3841 ggcttttgat gaattggacg ttggactcgg cataggcata gttgccgata acggtggaaa
     3901 aggcaaacat aaacaggatg acggcgagga agcccgcgcc ccattgcccc acttggctga
     3961 caatcgccgc ctgcgtcaac gccgcaccgc tcaaatcgcc gtaaggctgt tggtaaatca
     4021 agatgatgaa ggcggtgcaa gaacaaacga tgatggtatc gacaaacacg cccagcattt
     4081 gaatcatacc ttgcgaaaca gggtgtttca cttcggcggc ggcggcggcg ttcggcgcgg
     4141 aacccatacc cgcctcgttg gaatacaggc cgcgtttgat gcccatcatc atcgtttgcg
     4201 aaatcagacc gccgagtaag ccgcctgctg ccgcatcgaa tttgaacgcg cccgaaaaaa
     4261 tctgaccgaa cacgtccgga atcatcggaa tattggtcaa aatgatgaaa agcgcgataa
     4321 agaggtacaa aaccgccatc aggggcacga cgatttccgc cgctttagat atgcgcctga
     4381 tgccgccgaa gataatcggc gcggttaaaa tcaccagggc gacgccgaca taatgaggct
     4441 cccaacccca tgccgctttg acggtatcgg cgatggtatt ggtctgaacc gcttcaaaca
     4501 caaagccgaa acagaaaatc aggctcaggg cgaacaacac gcccagccat ttctgcccca
     4561 gcccttgagt gatgtagtag gcagggccgc cccggaaatg gtggttgtcg tagtcgcgga
     4621 ctttaaagag ctgcgccagc gaagattcga caaacgccga actcataccg attaaggcgg
     4681 ttacccacat ccaaaacacc gcgcccggcc cgccgacttt gatggcgatt gccacgcccg
     4741 cgatattgcc cacacccacg cggctggcaa ggccggttac aaatgcctga aacggcgtga
     4801 tgccgtgagg gtcgtccccc tgtttgcggc cgccgagcat ttctttgatg ctgcgcccga
     4861 acaggcggaa ttggacaaag cccgtggtta cggtgaagaa aagccccgta cccaaaagca
     4921 tataaaccaa gtatgaccac atcggatcgt tgatggcgcc gacccagccg tgcagccatt
     4981 cggtaaagtt ctcgttcata tcgcttcctt aaagttgaaa cccgcacata ttggcggtat
     5041 gcaagcaggg tttaaatttt gtaaacgccc attctagcag attgtcaaca aaatcagaaa
     5101 aatttacatc gccgcgcggc tgcggcgtta gaatcgcatt ttgtttggag caaacacgat
     5161 gaaacagcct gtttttgccg ttacttccgg cgagcctgcc ggcatcggcc ctgatatttg
     5221 tttggacttg gcgtttgcac gcctgccctg ccgctgcgcg gtattgggcg acaaacacct
     5281 gttgcgcgcg cgcgccgaag ccttgggcaa aagcgtcgtc ttgcgcgact tcgatccgga
     5341 atcaggcggc gcggcatacg gcgagctgga agtgctgcac attccgaccg ccgaagcggt
     5401 tgaggcgggc aaactcaatc ccgccaacgc cgcctatgtg ctgcaacttt tggacaccgc
     5461 gctcgcaggc atttcagacg gcattttcga cggcatcgtt accgcgccgc tgcacaaagg
     5521 catcatcaac gacgcgcgcg caagcacagg ttttttcagc gggcacaccg aatatctggc
     5581 ggaaaaaagc ggcacggggc aggttgtgat gatgcttgcc ggcaaagacc tgcgcgtcgc
     5641 cctcgtaacg acccacctgc cgctgaaaga cgttgccgcc gccatcacgc aaccgctgat
     5701 tgaaagcgtc gcacgcattt tgcatcacga cttaaaacac aaattcggca tcaaaaatcc
     5761 caaaatccta gtcgccggac tcaaccccca cgccggcgag agcggacacc tcggacacga
     5821 agaaaccgac accattatcc ccgcattgaa aaacctgcgc cgcgaaggga taaacctcgc
     5881 cggcccgtat ccggcggata ccttgttcca accgtttatg ctcgaaggtg cggatgccgt
     5941 attggcgatg taccacgacc aagggctgcc cgtgttgaaa taccacagct tcggacaggg
     6001 cgtgaacatc acactggggc tgccctttat ccgcacctcc gtcgatcacg gcaccgcgct
     6061 cgatttggcg gcaaccggca gggcggattc cggcagcctg ataactgccg tggagaccgc
     6121 cgtcgagatg gcgcgcggca gcctttaaag atgataaaag acccgtcatt tccgcgcagg
     6181 cgggaatccg gtctgttcgg tttcagttgt ttttgggttt cgggtaattt ccaaatcgtc
     6241 attcccgcgc aggcgggaat ccagaccttc gggcgacggc aatattcaaa gattatctga
     6301 aagtttgagg ttctagattc ccgttttcac gggaatgacg aaaggttgcg ggaatccggc
     6361 ttgttcggtt tcggtttttt ttgaggtttc gggcaacttc taaaccgtca ttcccgcgca
     6421 ggcgggaatc caggcctttg ggcgacggca atattcaaag attatctgaa agtttagagg
     6481 ttctagattc ccgttttcac gggaatgacg gaatgttgtg ggaatccaga cctttgggcg
     6541 gcggcaatat tcaaagatta tctgaaagtt tgaggttcta gattcccgtt ttcacgggaa
     6601 tgacggaatg ttgcgggaat ccagaccttc gggcggcggc aatattcaaa gattatctga
     6661 aagtttagag gttctagatt cccgttttca cgggaatgac ggaatgttgt gggaatccag
     6721 acctttgggc ggcggcaata ttcaaagatt atccgaaagt ttgaggttct agattcccgt
     6781 tttcacggga atgacgaaag gttgtgggaa tctagacctt tgggcggcgg caatattcaa
     6841 aaattatctg aaagtttaga ggttctagat tcccgttttc acgggaatga cggaatgttg
     6901 cgggaatcat gggaatggag gaatgtttcg gtaatcacgg gaatggcgaa gtttcagacg
     6961 gcattgcagg tatccgaacc catgtaaaaa agaggttctg cggaacagaa cctctttttg
     7021 ccgccgtcgg ttcagccttg ccgggtttcg acttggatca tttcttcggc agggacggtt
     7081 gcgacttcag acggcttggg ctgttcggaa cggcgcaaac cgcgtccggc ttggacttcg
     7141 ggttgtgccg cccatgcctt caatgcggca gggtcggttt cgatcaggac gagtccgctg
     7201 gtttgtgcgg tttcccgtgc ctgttccgcc gcagccgtaa aggttgcggt ttcagacggc
     7261 atttcctgtg cttcggcttt cggtgtcgcg ccttcgggca ggatggcggc ggtggcacgg
     7321 cggatttttt ccgccgcatc ataaaccggt gcgtcgccgt ttgaaacggc gggagatgct
     7381 gtcggaagat ccctttctgc aaccggatcg gcaatgctga cggtaatcgg cgcgtttgcg
     7441 tcggtttcgc cgaaaacgtg cgcggcggcg gaacggactt tgtcggcggt gtcgtgaata
     7501 ttcaggtact gctcgatttt ggcggcagac ggaatattgc gttttttgcc gttttgacgg
     7561 cggtcgcgct gattgttgcg ctcgcggcgt tctttggcat ctcggctgtc gcgttcgcgg
     7621 cggttgcgtt cggatttggg cttgctgcct ttgtcttctg cggtatgcgg ttcggacggc
     7681 gtgttttccg ctgtctgaac ggttgtttcg gcaacggtgg cggtttccgg cgcggtttgg
     7741 ccgcgttcgc tgcggctgcc gttgcggcgg cgttttccgg tttgcacttc ggtttcggac
     7801 ggtgcggcat ctgcaacggt tgcggcaggc tgtatgttgc ggtcttggat ttccgcttcg
     7861 tcggcgcgtc cggcggcacg gtcgccgcgt tcgttgcggc ggcggttgcc gttgttgcgc
     7921 gtttcggctt tgtcggcacg cgcttcctgt ccggcagttt tgcctgccac ttcgcggact
     7981 tctactttgc tgccttcgcg tttgctgcgg cgcgggtttt ggcggcggtt gttggcgcgg
     8041 ctgccgctgc ggtttgccgt gctgcgtttt tcggaggttt cggcagcggg cgcggcttgg
     8101 gtttcgctgc cgccgaaaat gcgtttgagc caagctttga agctgtccca ccaggaggtt
     8161 tttttctcgg gggcggcagt cggggcgggg ctggtgtggc gcacgccttt gacggcgggt
     8221 tcgggacggg cggctttggc tttttcgccg ccgaacggtt tggcggattc gtcttcttca
     8281 ggctcggcga cgcgtttgta gctcggttcg ccgtcttctt ctacgtcgtc ggtgcggatg
     8341 cggttgattt cgtagtgcgg attttcgagg tggatgttcg gaatcaggac gacgttgaca
     8401 tccaaacgct cttccatcgc aaacagctcg gcgcgttttt cgttcagcag gaaggtggcg
     8461 acatcgacgg gcacttgtgc gcgcacttct ccggtgttgt ccttcatcgc ttcttcttga
     8521 atgatgcgta aaacgtgcag ggcggtggat tcgatgcccc gaatcacgcc ggtgccggcg
     8581 cagcgcggac aggcgacgtg gctgctttca cccaaagccg gtttcaggcg ttggcggctc
     8641 aattccaaaa gtccgaaacg ggagagtttg cccatctgca cgcgggcgcg gtcttttttg
     8701 agcgcgtcgc gtaggacgtt ttccacatca cgctggtgtt tggagttttc catgtcgatg
     8761 aagtcgatga cgaccaagcc gcccaagtcg cgcaggcgca tttgtcgggc gacttcttcg
     8821 gcggcttcca tattggtttt gaaggcggtg tcttcgatgt ccgcaccgcg tgtggcgcgt
     8881 gcggagttga catcgatgga gacgagggct tcggtgtggt cgatgacgat tgcgccgccg
     8941 gaaggcaggc tgacgctgcg agaaaacgcg ctttcgattt gatgttcgat ttggaagcgg
     9001 gaaaacagcg gcgtgtggtc ttcgtagagt ttcagacggc ctatattgcc cggcatgacg
     9061 tagctcatga actcggcaac ttggtcgtaa acttcttgat tgtccaccaa aatctcgccg
     9121 atgtcggggc ggaaatagtc gcggatggcg cggatgagca gcgagctttc cataaagagc
     9181 aggtaagggt cgtgatgcgc ttttcctgct tcttcaatcg cctgccagag ttgtttgagg
     9241 tagttcaagt cccattccaa ctcttccgcg ctgcggccga tgccggcggt acgggcgatg
     9301 atgctcatgc cgtttggaat gtcgagttcc gccatggcgg ctttcaactc ttgacgctct
     9361 tcgccttcga tacggcggga tacgccgccg ccgcgcgggt tgttcggcat caataccaga
     9421 tagcgtccgg cgaggctgat gaaggtggtc agtgccgcgc ctttgttgcc gcgctcgtct
     9481 ttttcgactt ggacgatgac ttccatacct tctttgagca cgtcctgaat acgcgccctg
     9541 ccgccttcgt agtcgcggaa atacgaacgc gatacttctt taaacggcaa gaagccgtgg
     9601 cggtcggttc cgtaatccac gaaacacgct tccagcgacg gctcgatgcg ggtaatgatg
     9661 cctttgtaga tattgccttt gcgctgttct ttgcccagcg tttcgatgtc caaatccagc
     9721 aggttttgtc cgtcgacgat ggcaacgcgt agctcttcgg cctgcgttgc gttgaataac
     9781 attcttttca tgatcacctc gtggacgggc ggcattcggg cgcgccggtc cggttcggca
     9841 ttccgtaagg ctgggttttc cgatgtcttc ggataaaacc ggtaatcagt ttttgagttg
     9901 aaaatccgca gggatgcacg ttccggagaa ccgtgtgcgg aagggtcgga tacagaaggc
     9961 tataaagatc gatgcggcgg tctgtctgcc gcgttccgaa cgctgcggtc ggaaaaatgg
    10021 gggccggctt cttcttgtta tcgtgatgcc tgtgttttgg gcggtttgcg tttgggactt
    10081 ggggcccggc tgccgtctta cttccgcgcc gaaacggcaa aatcaattca aacttgatta
    10141 cgttctgcgc ctgccggctg ggaacaggcg cagggaaaat gctttgcgga gtgcgttttt
    10201 aatataaaat tccgttttaa agtaaaccgt ttcagggggg cgcggcgggc gcgctttttg
    10261 ctgaaacgga tgttcggatt atagatgaaa acgcacgaaa taagcaaaga ttcggtcagc
    10321 ttgatagggg ttgccgaaca cgaggcgggt caacgccttg ataactatct gataaaaatc
    10381 ctcaagggtg tgcccaagag ccatatccac cgcattatcc gcgccggcga ggtgcggttg
    10441 aacaaaaaac gctgcaaacc cgacagccgt attgcggagg gggatacggt gcggattccg
    10501 cctgtgcgcg tggcggagaa ggaaatgccg tctgaaaggc gtgccgccgt gccggcgcgc
    10561 gaatttgaca ttgtttacga agacgacgcg ctgctggtga tcgataagcc ttccggcgtg
    10621 gcggtgcacg gcggcagcgg cgtgagtttc ggcgttatcg aacagttgcg ccgcgcccgt
    10681 ccggaggcgc gttatctcga actcgtccac cgtttggaca aggatacgag cggcttgttg
    10741 atggtggcga agaaacgcag cgcgctcgtc aaacttcacg aagccatccg taacgaccac
    10801 cccaaaaaaa tctaccttgc gctgggggtg ggcaaactgc cggacgacaa tttccatgtc
    10861 aaactgcccc tgttcaaata taccggcgca caaggcgaaa agatggtgcg cgtcagtgcg
    10921 gacgggcagt cggcgcatac ggtgttccgt gtgttaagcc gtttttcaga cggcattttg
    10981 cacggtgtcg ggctgtcgca cctgactttg gtgcgggcga cgttgaaaac ggggcgcacg
    11041 caccaaatcc gcgtccacct gcaatctcaa ggctgtccga ttgcgggcga cgaacgctac
    11101 ggcgattatc aggcgaaccg tcgtttgcag aagttgggtt tgaagcggat gtttttgcac
    11161 gcgtccgagc tgcacttgaa ccatccgctc acgggcgagc cgctggtgtt gaaggcggag
    11221 ctgccgccgg acttggcaca gtttgcggtg atgttggaaa acgggacgaa aatgtgaacc
    11281 ccgatgccgt ctgaagcctt cagacggcat cgggacgtga aagtatgtgg ggacagacga
    11341 atatggttga taaaaaaagc cctttgattg ccgtcagtgt cggcgaagcg tcgggcgacc
    11401 tgttgggggc gcacctgata cgcgccatcc gcaagcgttg tccgcaggcg cgttttgtcg
    11461 gtatcggcgg cgaactgatg aaggcggaag gtttcgagag cctttatgat caggagcggc
    11521 tggcggtgcg cggctttgtc gaagtggtca ggcggctgcc ggaaatttta cggatacgca
    11581 gggggctggt acgggatttg ctgtcgttga aacctgatgt ctttgtcggt atcgatgcgc
    11641 ccgattttaa tttgggtgtg gcggaaaagc tgaaacggtc ggggattccg accgtgcatt
    11701 atgtcagccc gtcggtgtgg gcgtggcggc gggaacgtgt gggcaaaatc gtgcatcagg
    11761 tcaaccgcgt gttgtgcctg ttcccgatgg agccgcagct ttatctcgat gcgggcggac
    11821 gtgcggagtt tgtcggtcat ccgatggcgc agcttatgcc cttggaagac gaccgtgaaa
    11881 cggcgcggca aactttgggc gtggatgccg gcatccccgt attcgccctg ctgcccggca
    11941 gccgcgtcag cgaaatcgac tatatggcgc cggtgttttt tcagacggca ttattgttgt
    12001 tggaacgcta tcctgccgca tgcttcctgc tgcctgccgc aacggaggcg acgaagcggc
    12061 gtttggcgga agttttgcag cggccggagt ttgccggatt gccgctgacg gtaatcgaca
    12121 gacagtctga aacggtgtgc agggcggcgg atgcggtgct ggtaacgagc ggtacggcaa
    12181 ctttggaggt ggcgctgtgc aagcgtccga tggtcatcag ctacaagatt tcgccgctga
    12241 cttatgctta tgtgaaacgc aaaatcaaag tgccgcatgt cggcctgccg aatatcctgt
    12301 tgggtaagga ggctgtgccg gagctgcttc aacatgatgc cgtaccggaa aaactggcgg
    12361 cggcgttggc ggactggtac gaacaccccg ataaggttgc cgcgctgcaa caggatttca
    12421 gggcgttgca cctgctgttg aaaaaagata cggcggattt ggccgcgcgc gcggttttgg
    12481 aagaggcggg atgttgagcg gttaatggat tatcttcttg aagcagcacg tattacaaaa
    12541 aaagggggga gaaattgtga ttaatggcac atcaaacaat aagtatttaa gaggaattcc
    12601 aaatgaagga gaactggctc gagtgggatt aaggttaaaa tataatgggc cgttaaagga
    12661 agagtttaaa caactgaaat ttcataagag tagtagaacc aacttggact caaaaaatct
    12721 taaactcatt gtttttgaaa aggtaaaata atatgacaac ttataccatt ccaaaaaaag
    12781 attatcaatt tctgtatata tatgagggca ctctattaaa ctatactttg aaaaacgatg
    12841 aattccatat catcgtccag aatgtggatt atccggactt tcctcaagag attcctacac
    12901 caaattatac agactgggta aaaattaaat tcaagcagtt cagctatctg aaatttgtct
    12961 atggatacgc tacaaagagc caagatataa atatcgataa tacattggaa cttggcgaat
    13021 taaagcagga tgatgaaatc ttggattatg gaggtgcgct ggaattgata ggcggtaggt
    13081 atgattttcc gactgctttt aggatagata tagtttgccg ggaaatagag ttagaatttt
    13141 tagatcagga gagttttaat taaacgagcc gtaacttgtc atgccgcgca agcaacttta
    13201 ttgatatgcc gatacggagc ctgttggcaa aatgccgtct gaacaatatc ttttcagacg
    13261 gcattttgta tgggggttaa cggttgttca gcccgagtac gtcctgcata tcgtacaaac
    13321 ccgttttgcc gttgacccaa actgcggcgc ggacggcacc ggcggcaaag gtcatgcggc
    13381 tgctggcctt gtgggtgatt tccacgcgct cgccgtcggt ggcgaagagg gcggtgtggt
    13441 cgccgacgat gtcgcctgcg cggacggtgg caaagccgat ggtcgacgga tcgcgcggac
    13501 cggtgtggcc ttcgcggccg taaacggcgc attgtttgag gtctctgccg agcgcgccgg
    13561 cgatgacttc gcccatgcgt aacgcggtgc cgctgggggc atcgactttg tggcggtggt
    13621 ggccttcaat gatttcgatg tcgtagcctt cgtttaatac gcgtgcgacg gtgtcgagga
    13681 tgtggaaggt gaggttgacg ccgacgctga agttggcggc gaaaacgatg cctgtttgtt
    13741 cggcggcagt gtggatagcg gctttgcccg catcgtcgaa gcctgttgtg ccgatgatga
    13801 tgttgacttg tttttcaacg catttttgca ggtgtttgag ggtgggctcg gggcgggtga
    13861 agtcgatgag tacgtcgctt tgtgcgagaa cggcgtcaac gtcgtctgaa atggcgatgc
    13921 cggttttgag tccgacggcg tagcctgcgt ccagtccgag ggcttctgag cctgagtgtt
    13981 cgagcgcgcc ggaaaggacg gtgtcgggat ggttgttgac ggcttcaacc aatacgcgtc
    14041 ccatacggcc gtttgcgccg gcgatggcga ttttgagcgg tgtcatgtgt gttccttatt
    14101 gtttgtctgc gttttgtttt acgcggaggg cttcggcggc attttgcagg gcgttgcctt
    14161 cggtgcggac gagcacgccg ttttcaaaat agacggtcag attgcttcgg tctttgatga
    14221 tgccgttgcg ggaggtgttg aaggtatagt cccagcggtc ggtatggaat gcgtcgcgca
    14281 gtatggggct gccgagcagg agcaggactt ggtctttggt cataccgggg cggagggcgg
    14341 caacggcgcg ggggtcgatt tcgttgccct gtatgacttt gagcttgtat gaggggaaca
    14401 gcgaggcgcg ttcggcactg cacgcggcaa ggccaaggag ggcggaaagg gcgaggatga
    14461 gggttttgtt cacggaaatg cctttctgtg caaatcggga tgggtagtgt aacactgctt
    14521 gaatatttta taaaagcgaa cgataatcat acgattaagc ggtatccgcc ctgtccgcgc
    14581 atcggccgcc ggtgcggttt tactattgca aactgctatg gtgcgatagt gggcaaacag
    14641 gccgaaattg cgtattataa cgtctattgt tttacagggg tattgaatat tatggaaaaa
    14701 ttcaacaata ttgcacaact gaaagacagc ggtctgaagg ttaccggccc gcgtttgaag
    14761 attttggatt tgttcgagac gcattcggaa gagcatttga gtgcggaaga tgtgtaccgc
    14821 attttgttgg aagagggtgt ggaaatcggt gtggcgacga tttaccgtgt gctgacccag
    14881 tttgagcagg cgggtattct gcaacgccat cattttgaaa cgggcaaggc ggtttatgag
    14941 ttggacaaag gcgaccacca tgaccacatc gtctgcgtga agtgcggcga ggtaacggaa
    15001 ttccacaatc ccgaaatcga agccctgcaa gacaaaatcg ctgaggaaaa cggctaccgc
    15061 atcgtcgatc acgcgcttta tatgtacggc gtgtgcagcg actgtcaggc caagggcaaa
    15121 cgttaaatcc ggacggtttg ttgttcagac ggcattcatg attttggatg ccgtctgtgt
    15181 ttttggagaa ctgccatgcg tattccgctg cttgcccctg acaattatgc ctttcccgat
    15241 cctacctatg ctttggcccg gtgcgacggg ctggtcggcg tgagcggcga tttggatgcg
    15301 gggcggctgc tcgaggcgta tcggaacggc gtgtttccgt ggttttctcg ggacggttgg
    15361 tttttttggt atgcggtcgg cccgcgtgcg gtgattgttc ccgaacgtct gcacgttccg
    15421 cgctcgctgg cgaaaacgct gcgcaacggc agctatcggg ttgcggtcaa cggctgtttt
    15481 gcggaagtgg tcgcgcattg tgcggcagcg gcgcgcccga atcaggacgg aacttggatt
    15541 gcgcccgagt ttcagacggc atatttgaag ctgcacgaaa tgggacacgc gcattcgttc
    15601 gagtgccatt atcccgatga aagcggtgaa acgaggttgg cgggcggctt ttacggcgtg
    15661 caaatcggca gggtgtttta cggcgaatcg atgttcgcat tacaaccgga tgcgtcgaaa
    15721 atcgcgtttg cctgcgccgt gccgtttttg gcggatttgg gtgtggaact gatagactgc
    15781 cagcaggaca cggaacatat gcgccgtttc ggttcggagc tgctgccgtt tgcggatttt
    15841 gccgaacgtc tgcggatgtt gaacgccgtg ccgttgaaag aggaaatcgg gcggcgcgaa
    15901 gtggcgtgca aggggctttg atggcggctt atgctccggt caggttcaaa tatggtggat
    15961 taaaattgga aatggcgaca gccgggcaaa atcactgcca aaaaatgaaa aatgccgtct
    16021 ggaacttgaa aacatcgggt ttcagacggc attttgtttg acggtttatt gcttatttga
    16081 gcgggcgcac ttcaagtccg aacatacggc gtgcggtgtt cagcatttgg cagctgaagc
    16141 cccattcgtt gtcataccat gcgaagactt taatcatgtt gccgtcaacg actttggtca
    16201 gtgttgcgtc gaagtggctg gcttcggtgg tgtggttgaa gtccatggaa accaagggca
    16261 gggtgttgta gcctaaaacg cctttgagcg cgcctgcttc cgaggcggct ttcatcagtg
    16321 cgttgatttc ttcgattgtc gtgtcgcgcg cggcttggaa gttcaaatct accaatgata
    16381 cgttgacggt cggcacgcgg atggcaagcc cgtcgagttt gtctttcaat tcgggcagta
    16441 ccagtccgac ggcttttgcc gcgccagttt tggtcggaat catgttttcc acgccgctgc
    16501 gggcgcggcg caggtctttg tggcgcacgt cggtaacggt ttggtcgttg gtcagcgcgt
    16561 ggatggtggt catcgcgcct ttgacgatgc cgacgctttc gctcaacact ttggcaaccg
    16621 gcgagaggca gttggtggtg caggaagcgt tggaaacgac ggtcatgtcg gcggtcagga
    16681 tgctgtcgtt cacgccgtac acgacggttg catcgacatc gtcgccgccc ggtgcggaaa
    16741 tgaggacttt tttcgcgccg ctttccagat gaactttggc tttttctttg ctggtgaacg
    16801 cgccggtgca ttccatgacc aaatcgacac cgagttcttt ccacggcagt tcggcagggt
    16861 tgcgggtcga gaagaagggg attttgtcgc cgttgacgat gaggttgccg ccgtcgtggg
    16921 atacgtcggc ttcaaagcgt ccgtgcacgg tgtcgaattt ggtgaggtgg gcgttggttt
    16981 caaggctgcc gctggcgttg acggcgacga tttggagttg gtcttgaatc tgataatcgt
    17041 agatggcgcg caaaacctgg cggccgatgc gtccgtagcc gttgatggcg actttgatgc
    17101 ccatggtttg ttcctttgtt gaggggttgg gtagattttc ggggcggatt atagcaaatt
    17161 tgtagtggcg tgtaattaat attttattga aaacggcgcg gccggaaggg tgggcggtaa
    17221 gatgcggacg gcacgggtgc ggcggacgga gagcttgata aaatgccgtc tgaaacggtt
    17281 ttcagacggc atatcaggga agggtcagga ggcggtattc tgtgcggctt cctgtttggc
    17341 tttgtattgt ttgagatatt cgagggcggc ggctttttcg ctgtcgctgc cgtatttcat
    17401 atcgcgttgg gcgcggcgca actcggcgcg ttcgcgggct tcggctatct gtttcgcctg
    17461 atagtctttg cggttgtcgg cggcggcgag gcggtcttgt tgggtttgcg cttttgccat
    17521 ggctttggcg atgaggtcgg cagggttaaa cgccggtttt ttcggtgtgt cgggtgtttg
    17581 cggacgcgcg ttgcggacgg cggcttcgcg ttcggcaagc atggccttgc gttcgtcggc
    17641 ttcgcgctgt ttgcgttcgt tgcgtttgag gtagcgcgca cgcgcgtgtt cggcggcggc
    17701 aaaacggctg tcggcggaca ggctgaagcg gcgcgcgcgg ggcaggacgg tgtcggcaac
    17761 gggctgcata tggatgcagt cgacggggca gggggcgacg cagagtccgc agccggtgca
    17821 ttcgtcggcg atgacggtgt gcataagttt gcccgcgccc ataatggcat cggcagggca
    17881 ggcgcggatg caggcggtgc agccgataca ggcggtttcg tctatccggg cgagtgcttt
    17941 ggcttgggtt ttggcaggtg cgacaagggg tttgccgagc agggcggaaa tgtcccgaat
    18001 gacggcttct ccgcccgggg cgcagaggtt gtacgcttcg cctgttgcga ctgcctgtgc
    18061 gtagggcagg cagccgtcgt agccgcattc gcggcattgg gtttggggaa gcaggcggtc
    18121 tatggcggcg gctgtggcgg tcatgtcggt gtgcggctca aaatcgaaag ggcgtatttt
    18181 agcagaattg tatgccgcgc ccgtttcgga tggtgcgcgg tgttttgtta taatacggcg
    18241 gcgtatgccg tttcagacgg catttttctg tattttcctg ttcggacggc ctatgaacga
    18301 attttcgctt gcccctattg tggttgtttt gctggtgtcg gtcattacgg tgatcctgtg
    18361 ccgcaagttc aacattccct ccatgctggg ctacctgctg gtgggctttt tggcggggcc
    18421 cggtatgctc agcctgattc cgaaaagcca tgcgacggat tatttgggcg aaatcgggat
    18481 tgtgttcctg atgttcagca tcggtttgga gttctcgctg cccaagttga gggcgatgag
    18541 gcggctggtg tttgggctgg gcggtttgca ggtcggcgtt acgatgctgt cggtaatggg
    18601 catactgatg ctgacgggcg tcccgttcaa ttgggcgttt gccgtgtcgg gcgcgttggc
    18661 gatgtcgtcc acggcgattg taagccggat tttgtcggaa aagacggaat tggggcagcc
    18721 gcacggtcag atggcgatgg gcgtgctgct gatgcaggac atcgccgtcg tgccgctgat
    18781 gattctgatt cccgcgctgg cgggcggagg ggacggaaat atttgggcgg ccttgggttt
    18841 ggcgtttgca aaaatgctgc tgacgctggg gctgctgttt ttcgtcggca gcaaaattat
    18901 gtcgcgatgg ttcaggatgg tggcaaaacg caaatcgtcc gaactcttta tgatcaatgt
    18961 gctgctggta accttgggtg tggcttatct gactgagctg gaaggtttgt ctatggcgtt
    19021 gggcgcattc gttgccggca tgctgctttc ggaaacggaa taccgtttcc aagtcgaaga
    19081 cgacatccgc ccgttccgcg atattttgct cggctttttc tttatcacgg tcggcatgaa
    19141 gctggacatt caggcattga tcggcggctg gcggcaggta ttgatgctgt tggcaatgct
    19201 gctggtgttg aaggcactgg ttgtgtttgc cattgccttc aaaatgaaac attcggtcgg
    19261 cgacagcctc aaaacggctt tgtatctcgc gcagggcggc gagttcggct tcgtgatgct
    19321 ggccattgct gggcagcttg atatggtttc gccagaatgg gaacaggcgg cgacggcggc
    19381 ggttctgctg tcgatgatta tcgcgccctt cctcttgggc ggcagcgatg cactggtcgg
    19441 gcgtttggtc aagtcaagct gggacatgaa gtcgctcgat ctgcacagta tgctggtaga
    19501 aaccatgagc aagtccgacc atgtgctgat tgtcggcttc ggcaggggcg ggcagacggt
    19561 cggacgcgtc cttgcccaag aggatattcc gtatttcgcg ctcgacttgg acatcgcgcg
    19621 cgtgcaggtt gccaaaaatg ccggcgagcc ggtgtcgttc ggcgatgcga aaaggcgtga
    19681 agtgttggag gcggccggtt tgggacgggc gaaaatggtg gtggttacgc tcaacaatat
    19741 gcacgaaacg caacacgttt tagacaatgt gctgtccatg tatcccaata tgcccgtata
    19801 tgtgcgcgcc accaacgacg attatgtgaa aacgtttacc gatataggtg cggaagaagc
    19861 cgtgtcggac accaaagaaa ccggactcgt gctggcaggc tatgcgatgt tgggcaacgg
    19921 cgcgtcgtat cggcacgtct atcagacgat ggcaaatatc cgccacagcc gttatgccgc
    19981 gttggaggga ctgtttgtcg gtagtgatga tgaggcagga ttcggcgaaa atggcgaaac
    20041 cgtccgccac gcctttcctt tggctgcaga agcatatgcc gtcggcaaaa cagtcggcac
    20101 gcttccgatg gcggcttacg gcatcaaact cttgttcgtc cgccgccgca ccggtcggat
    20161 tgaaaacccg gatgcctcgt ttacattgga aggcggtgac gtgttggtgg tcgcaggcaa
    20221 aaaagaagaa attatctctt ttgaaaactg gagtttgcag ggaatataaa tgaaatgccg
    20281 aaataaggct tgcgccattt ccggttattt ggtttaataa cgctttcgca aatcgca
//
LOCUS       DAWFKN010000030        19944 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFKN010000030 DAWFKN010000000
VERSION     DAWFKN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.030"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(163..363)
                     /locus_tag="WGR46_001084"
     CDS             complement(163..363)
                     /locus_tag="WGR46_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856036.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(377..931)
                     /locus_tag="WGR46_001085"
     CDS             complement(377..931)
                     /locus_tag="WGR46_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856037.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1016..1516)
                     /locus_tag="WGR46_001086"
     CDS             complement(1016..1516)
                     /locus_tag="WGR46_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856038.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1920..3113
                     /locus_tag="WGR46_001087"
     CDS             1920..3113
                     /locus_tag="WGR46_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0856039.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(3178..4422)
                     /gene="clpX"
                     /locus_tag="WGR46_001088"
     CDS             complement(3178..4422)
                     /gene="clpX"
                     /locus_tag="WGR46_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0856040.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4613..4984
                     /gene="rbfA"
                     /locus_tag="WGR46_001089"
     CDS             4613..4984
                     /gene="rbfA"
                     /locus_tag="WGR46_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0856041.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            4995..5519
                     /locus_tag="WGR46_001090"
     CDS             4995..5519
                     /locus_tag="WGR46_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856042.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            5577..6497
                     /gene="truB"
                     /locus_tag="WGR46_001091"
     CDS             5577..6497
                     /gene="truB"
                     /locus_tag="WGR46_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0856043.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            6817..8963
                     /locus_tag="WGR46_001092"
                     /pseudo
     CDS             6817..8963
                     /locus_tag="WGR46_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            8953..11733
                     /locus_tag="WGR46_001093"
     CDS             8953..11733
                     /locus_tag="WGR46_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0856044.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(11877..13049)
                     /locus_tag="WGR46_001094"
     CDS             complement(11877..13049)
                     /locus_tag="WGR46_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0856045.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            13339..13785
                     /gene="iscR"
                     /locus_tag="WGR46_001095"
     CDS             13339..13785
                     /gene="iscR"
                     /locus_tag="WGR46_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0856046.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13814..15028
                     /locus_tag="WGR46_001096"
     CDS             13814..15028
                     /locus_tag="WGR46_001096"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0856047.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            15289..15675
                     /gene="iscU"
                     /locus_tag="WGR46_001097"
     CDS             15289..15675
                     /gene="iscU"
                     /locus_tag="WGR46_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0856048.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15762..16082
                     /gene="iscA"
                     /locus_tag="WGR46_001098"
     CDS             15762..16082
                     /gene="iscA"
                     /locus_tag="WGR46_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0856049.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(16042..16263)
                     /locus_tag="WGR46_001099"
     CDS             complement(16042..16263)
                     /locus_tag="WGR46_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0856050.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            16345..16845
                     /gene="hscB"
                     /locus_tag="WGR46_001100"
     CDS             16345..16845
                     /gene="hscB"
                     /locus_tag="WGR46_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0856051.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16943..19693
                     /gene="gyrA"
                     /locus_tag="WGR46_001101"
     CDS             16943..19693
                     /gene="gyrA"
                     /locus_tag="WGR46_001101"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0856052.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19850..>19944
                     /locus_tag="WGR46_001102"
     CDS             19850..>19944
                     /locus_tag="WGR46_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0856053.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVG"
BASE COUNT         5597 a         5021 c         4662 g         4664 t
ORIGIN      
        1 tataaaaacc cttttccgct ttggcaacga ttgaaaattt ccgtaaattc aaatatctag
       61 attccttcct gcacgggaat gacacggaag ggtttcagat gcagggtggg cattcctgcc
      121 caccaaatcc cgcccttgca acggtgggca agaatgctcg ccctacggct tgactgttcg
      181 atatgatgcc gtctgaaaac ccaacggcgg catgacaatg ccgccctgcc aacgcacgta
      241 aatcagaatt gccatcccga catcaaacgc ttggaaacaa aatgccgtct gaaaatcaaa
      301 cggcaacata acaatgtccc taacaaatgc aaaaatgccg tctgaaagct cttcagacgg
      361 cattggtgcg ccgggtttac cgcctcctgc cgaaaccgcg catagcgggg cggcggtaat
      421 tggcgggcgg gcgcgttgtc gggcggtaac gctgcgcctg cgccgcctgt tgttttgcac
      481 ggaggctgcg cgtgttcaaa tccctgctgg tgcgcgcatt gggatgtgcg gactgatggt
      541 aggcggcacg cgcgcgccgg gcgacgggac tgtcttggtt gcctgcccgt gtgaatttgt
      601 ttgccagcgc gttgccgata aacgcgcctg ccgccgcgcc gaccaggctt tgcagcagcc
      661 agcttcctgt cgattggtcg taaatatact gctgcccgtc tttaccggta acgggttgcc
      721 cgttgttgcc gtttgcctgt gcttcggcag gaatggtgtc tttgactgct tcgggagtca
      781 gttggtaaac cgtatcgtct gcctgctgtg cgagctgctg ttgcagggct tcaatctgtt
      841 tctgctgctg ttcgagccgc gcctgcgtgt cgtcttggca ggcggcgagt gcgaatgttg
      901 cgataagcgc ggaggcgatg atttttttca tgtgtgtcct gtttgggtgg aaaatcggtt
      961 ttattgtatc gccgtcggga attttggcaa gcattctgcc gacaaatcgt gatgtttaca
     1021 ggggcagggt gtgcagtttg cggacaaatg cgaggctgtt ggcgactggg ttgcctttgt
     1081 tttcgacttc gtgttcggtt tctacggtca gcaggcggcg gtttttgtgt ttgttcaggc
     1141 tcaattcggc ttgtgcgggt gtgaaaaaca ggcggtgttt ttcggcaaag cggcgggcgc
     1201 ggctgttggc ggttttcaaa atctgaagca gcgatacgtc cagatggaag cgtcgcacgc
     1261 ccaatacgag aatccgtgcg gcaaaaaaat cggcggcatc ggtaaactcg gcggggctgc
     1321 tgcggctgaa gtcgtgccgt tcggcggcta tcagggcggc aacagtgcgg atttgcggaa
     1381 tcatcgattc gctgatgagt gtgtcgtccc gccctgcatc gagaagcatg ggggaaaaat
     1441 cctgtgcatc atcgacaata atgctacaag tgtgcagggt ttcgttttgt gcggcggttt
     1501 ggggcattgc attcatggtc attttcctga ttctgtcgtg tgttgccgaa tcgggcgacc
     1561 tgtgtgaagg taacaaaaaa gccgccccgt tttcgagcgg cctgttttgc gtatgggatg
     1621 gatttcaagc aagcgcaaaa aagtaccgca cgtctgtgtg gtaccaatag caataagcgg
     1681 ttgtaaattt tttgccttgc atgatgaaat gccgtctgaa gataaaaata ttggggagat
     1741 tctaaatcaa aacgctgccg cgcctcaagc attttatcga aatttttttg atttttcacc
     1801 tatccgattg aaaatatttc ggtttatttt taccgctgcc cgatattgtc ggcaatttcc
     1861 ctttatctgc tttgaaaaac ggtgcataat cccgagcaaa accgcaatca ggagcaatta
     1921 tgcaaaacta tctgaccccc aatttcgcct ttgccccgat gattcccgaa cgcgcttcag
     1981 gcagccgcgt ttgggatacg gaagggcgtg aatatattga tttttcaggc ggtatcgccg
     2041 tcaatgcgct gggacactgc caccctgccc ttgtcgatgc tttaaacgcg cagatgcaca
     2101 agctgtggca catttccaat atctatacga cgcgtccggc gcaggaattg gcgcaaaaat
     2161 tggttgcaaa cagttttgcc gacaaggttt ttttctgcaa ctcgggcgcg gaagcgaatg
     2221 aggcggcgtt gaagctggca aggaaatatg cgcgcgaccg tttcggcgga ggaaaaagcg
     2281 aaatcgtcgc ctgtatcaac agtttccacg gacgcacgct gtttaccgtg tccgtcggcg
     2341 gccagccgaa atacagcaag gattatgcac ccctgccgca aggcattacg cacgttccgt
     2401 tcaacgatat tgccgcgctg gaagctgccg tcggcgaaca gacctgcgcg gtcatcatcg
     2461 agccgataca gggcgaaagc ggcatcctgc ccgccactgc ggaatatttg caaaccgcgc
     2521 gccgtctgtg cgaccggcac aatgcgttgt tgattttgga cgaagttcaa accgggatgg
     2581 ggcatacggg caggctgttt gcctatgaac attacggcat tgttcccgat attttgagtt
     2641 cggcaaaagc cttgggctgc ggctttccga tcggcgcgat gctggcgaca gaaaagattg
     2701 ccgccgcctt ccaaccgggc acgcatggct cgactttcgg cggcaacccg atggcgtgcg
     2761 cggtcggcag ccgcgccttc gacatcatca acgcgccgga aaccttgcac aacgtccgca
     2821 gccaggggca gaaacttcag acggcattgc tggatttggg caggaaaaca ggcttgttct
     2881 cacaggttcg cgggatggga ctgctgctcg gctgcgtgtt ggacgcgcct tatcgcggac
     2941 gcgcatccga aataaccgcc gccgccttga aacacggcgt gatgattttg gttgcgggtg
     3001 cggacgtatt gcgtttcgcg ccttcgctgc tgttgaacga tgaggatacg gcggaaggtt
     3061 tgcggcgttt ggaacacgtg ctgacggaat ttgccgccgc aaaccgcccc taggcatcaa
     3121 aaacaaaaaa ccgcttcgga aacgtggttc aacgttccga agcggttttg tttgccatca
     3181 ggactcgaac accaattccg gttccctgcc ctcttcgatg actgccttac cgaccaccac
     3241 ttttttcaag ccttgcaaat caggcaggcg gtacatcgta tcgagcaggc agcgttcgac
     3301 gatggacctc aggccgcgcg cgccggtttt gcgctccatt gcctgacgcg cgatggaacg
     3361 caatgcgcct tcttcaaatt ccaactcgac attttccata ccgaacaagg cttgatactg
     3421 cttgaccaaa gcatttttcg gctcggtcaa aatattaatc agcgcgtcct catccagttc
     3481 ttctagtgtt gcaatcacgg gcaaacgtcc gattaattcc ggaatcagac cgaatttaat
     3541 caagtcttcc ggttcgacga tgccgaacag cttggtaatg tcggcatttt cgtccttgct
     3601 gtgaacggac gcaccgaaac cgataccgcc tttttcagta cgctggcgga tgactttttc
     3661 caagcctgca aacgcgccgc cgcagataaa caggatgttg gtggtatcga cgttgataaa
     3721 ttcctgattc ggatgcttgc ggccgccttg gggcggaacg ctggccactg taccttcaat
     3781 cagtttcaac aaggcttgtt gcacaccttc gccagatacg tcgcgggtaa tcgacgggtt
     3841 gtcgcttttg cgtgaaattt tatcgatttc gtcgatatag acaatgccgc gctgggcttt
     3901 ttcgacatcg aaatcacatt tgcccaacag tttggtaata atctgttcga catcttcgcc
     3961 gacataacct gcttcggtca gcgtggttgc atctgccatc acgaacggca catccagttt
     4021 gcgcgccaaa gattgcgcca gcagggtttt acccgatccg gtcgggccga taagcaggat
     4081 gttggatttc gacaattcga cattagctcc ggctttcggg tggcgcaggc gtttgtaatg
     4141 gttgtacacc gacaccgcca aggctttctt ggcttgttcc tgaccgataa cgtggtcgtt
     4201 gaggttggcg acgatttcgg cgggcgtggg cagcttgccg gattcttccg gctcccctcc
     4261 ggcactttcc gaaggcgtgc cgtcatttcc gtcttcatgc aatatttcaa tacagtttga
     4321 gacgcattcg tcacagataa aggcgttttc gccctcaatt aaatgtttga cgtgtgattt
     4381 ggattttccg caaaaggaac aggtacggtt ttcgttggac atggctttct ttacaatgta
     4441 tgcgttacag aaaacggcac gtgccgttcg ggttgccaag tataataact atatccgttc
     4501 ttatcaatgt attaccttaa aatcccgccg attaggctat aatacgccct ttcgcaaccg
     4561 ccccggcggc aaaaatgccg tctgaaacca aatctgaaat ctgaggatat tcatgagaaa
     4621 accccaacgc ggctacgccc gccaagaccg tgtcaaagaa caaatcatgc gcgagcttgc
     4681 cgaactcgtc cgtaccggac tgaaagaccc ccgcgccggc ttcatcaccg tcaacgaggt
     4741 cgaagttacc cgcgattaca gccacgccac cgtgttctac accgttttaa accaagatgc
     4801 gcgcgaaatt acggaagaag tgctggaaca cgcacgcgga cacctccgca gcgaattggc
     4861 caaacgcatc aagctgttca aaatccccga gctgcatttc aaatacgacg aatcgttgga
     4921 acgcggtttg aacctgtccg cccttatcga tcaagtggcg gcggaaaaac cggttgaaga
     4981 ctgatactgc taaaatgaac aatctgattc ccgaacattt agccgcctat gcacatagtg
     5041 acagcctgca aattgaaggc gtgcatcgtt gcttttcatt atcctgccaa ggtagagata
     5101 ctttccacat ccgttactat ggagagcctt ttgatggatt gatgaccgat actgataagg
     5161 ctccggtaaa aattgtggca gtagaagctg taagcggcga tgaaatcgta ttgtttgatg
     5221 gcgcggaaca tggctataac gctatgtttt gcgacaaata tagtccaaat caaaagcaaa
     5281 acagaacgtt aactgatttg gatgagtata cttaccgagt tctgatccat ctttattaca
     5341 atatagacta tgaagatgaa tatgaagatt tcgtcaattc tgaaggacaa gtccccttaa
     5401 ttgatggtcg aatcattagt tttgactcat taaaacgaaa tggctttgat gcaatcagcg
     5461 ttgatctaat tgatgaaaaa cactctgttc gtgaattatt gaatgaagaa ttaagctgac
     5521 acgcaggccg tccgaaccct ttcagacggt ctgaacatta atattcccta attaatatga
     5581 ccaataaacc cgccaaacgc ccggtcaacg gcgttcttct tctcgacaaa cccgaaggcc
     5641 tttccagcaa caccgccctg caaaaaacga ggcacttgtt ccgcgccgaa aaagccggac
     5701 ataccggcgt gctcgatcct ttggcaaccg ggcttttgcc cgtctgcttc ggcgaagcga
     5761 ccaagttcgc ccaatatctg attaatgccg acaaagccta caccgccaca ctgaaactcg
     5821 gcgaagccag cagcacgggc gatgccgaag gcgaaatcgt tgccaccgcc cgcgccgata
     5881 tttccttagc cgaatttcag acggcctgcc aagcactgac aggcaacatc cgccaagtgc
     5941 cgccgatgtt ttccgcgctc aagcacgaag gtaaaccgct gtacgaatac gcccgcaaag
     6001 gcatcgtcat cgaacgcaag gcgcgcgaca ttaccgttta cgccatcgat attgccgaat
     6061 ttgacgcgcc caaagccgtc atcgacgtac gttgcagcaa aggcacctat atccgcaccc
     6121 tcagcgaaga catcgccaaa cacatcggca cgttcgccca cctgaccgcc ctgcgccgca
     6181 ccgaaaccgc cggctttacc atcgcccaaa gccacacgct cgaagccttg gcaaacttgg
     6241 acgaaacaga acgcgacagc ctgctgctgc cctgcgacgt attggttgcc cattttcccc
     6301 aaaccgtttt aaacgattat gccgtccata tgctccgctg cgggcagcgg ccgcgtttcg
     6361 aggaagacct accttccgat acgccggtac gcgtttacac ggaaaacggc cgctttgtcg
     6421 gtctagcgga atatcaaaag gaaatatgcc gtctgaaagc cttgcgcctg atgaacacgg
     6481 cggcatccgc cgcctgaacg acggttaaaa atacgggctg tgtttgaata atgtgttgat
     6541 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tttgtactgt
     6601 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatttccg caaaatcacg
     6661 acacactcgg acacccgccc cgcttatcgc aactttgcga acgcccccgg aaacagcaaa
     6721 gacatcaaat aattgatttt attagaatct atttgcaaag ccatttgccg ttacacaaga
     6781 atggcacatt aaaataactg atgaggattt ataacgatga agacagacat tcaaaccgaa
     6841 ttaacccaag ccctactacc acacgaaaaa gtatgggcga acgaagaaaa aaccatttta
     6901 gccaaaaaca tcctgttgga tttggtggaa aaaaccgacc cgaccattat cggtttgtta
     6961 ttgagtaatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt gctggtgttt
     7021 aaattgcagg atttccgttt tttcttggac aaacacagcg tcaataattc ctacacaaaa
     7081 tacgccaacc gcattggttt gacggacagc aaccgctttt tgaaagacag cagtgatatt
     7141 gtgttggatt ttccgtttaa agattgtgtg ttaaatggcg gacaaagcac cgaggaaggc
     7201 gaagaaattt attttaaacg caataacagc cagccagcca attatacacc aaattaaccc
     7261 gaaaaagaca agaaatcttt tttaatcaaa cgcttgcttt tgatgaaatt gaccgacttt
     7321 ttgacgcaaa agcattctca aaattctctc gctataccgc agacggcaaa caagcggttg
     7381 gcgaaatcaa acgccattca gacggcacac cggccgaaaa tcttattatc aaaggcaata
     7441 atctgattgc cctgcattcg cttgccaagc agtttaaagg caaagtgaag ctgatttata
     7501 ttgatccgcc ttattatttt gttaaaaaga aacaacaaga tagttttggt tataacacta
     7561 actttaaatt atctacttgg cttacattta tgaaaaatcg tttatcaatt gccaaagaat
     7621 tattaactga tgatgggatt attgttattt ccattgatga tgacggaaat gcttatttaa
     7681 agattttgtt agatgaaatt tttggatttg aaaattttat cggaaattta cccactatta
     7741 tgaatttaaa aggtaataat gatgaatatg cttttgcagg cactcacgaa tacacactag
     7801 tctttgctaa aaataaagat aaatctactt tttatgaatt tccaatagat gaagataatt
     7861 ttttagagaa atgggaagaa gatgaaattg ggttttataa aaaaggagct ccaatgcgag
     7921 caacaggaac cgaagataaa agagaagata gacctgaaat gttctatcct tttttggtaa
     7981 aaaataacac agttagtaca attacagatg aagaattttc tcaaatctat aataaagatt
     8041 tagaagtatt caatgatgat tttattcaaa aactaaaaga gaaatacgaa aatcttggat
     8101 acaattttat tttacctatt gctaataaac aatgggggcg ttggcgttgg gggtattcaa
     8161 taaagaataa ggcaaggctg cagacggata ttattgttag ccaatctaaa aatggaattt
     8221 ccttgtataa aaaacaaaga cctgaattag atgatttacc tacaaaaaaa cctaaaacta
     8281 ttttttataa acctgaatac agtagtggta atggaactga acagatgaaa aatctttttg
     8341 gtgaaaaggc atttaaaaat cctaaaccag aagaacttat ccaagatttt attaccatca
     8401 caaccaacga aaacgacatc gtcctagact accatttagg cagtggcaca accgccgccg
     8461 ttgcccacaa aatgaaccgc caatatatcg gtattgaaca aatggattat attgaaacgc
     8521 ttgccgttga acgcttgaaa aaagtgattg atggcgagca aggcggtatt tccaaagccg
     8581 tgaattggca aggtggtggc gagtttgttt atgccgaact tgccccattt aacgaaaccg
     8641 caaaacaaca aattttggct tgcgaagatt cagacggcat caaaacgctg tttgaaggtt
     8701 tatgcgaacg ctatttcttg aaatacaacg tcagcgtaaa tgaatttagt caaatcattc
     8761 aagagcctga atttcaatcc ttggcattag acgaacaaaa acaaatggtg cttgaaatgt
     8821 tggatttaaa tcaaatgtat gtttcattat ccgaaatgga tgatgagcaa tttgcaggtt
     8881 gcttaaatga tgacgataaa gcgttaagcc gtgaattcta tcaatcagca aaaaatcaag
     8941 cggagaaaaa agatggcgaa taataaaacg ttatttgatt gggtggaaga ccgcaaatca
     9001 acgctggaag aaatagaaca gacagatttt ttcgcgctgc ctgaatttgt agaacgcaat
     9061 ttaaaatatc cgttttttga atggcaaaaa tcggctttag aaaactttgt gatttttgac
     9121 cgcgcttcaa aattaaagga tttccctgac atcaaaaacc gccctaccca tttgctgttc
     9181 aatatggcaa caggtgctgg caaaacgatg atgatggcgg cgttgatttt gtattatttt
     9241 gaaaaaggtt atcggcattt tttgtttttc gtcaatcaaa ataatatcgt ggataaaacg
     9301 gaaaataatt ttattgaccc ggcgcacgca aaatttttat ttaccgagaa gattttgcaa
     9361 ggtgatacgg taattcctat tcgcaaagtg gaaatgttca gcccacattc agacggcatt
     9421 gaaattaaat ttaccagcat tcaaaaactg tataacgata tccacaccga gcgagaaaac
     9481 caaaccacat tggcagattt gcacaaattg aaccttgtga tgctgggcga tgaagcacac
     9541 catttaaacg cacaaaccaa aggcaaaaaa caagacgagt tggatttgga agtggaatta
     9601 aaagccaatg caggcaatgc cgaaattgaa cgcaaaggct gggagcatat ggttttggaa
     9661 ttgttgctca ataaaaatgg caatcccggc caaaatgtgc tgttggaatt taccgccacg
     9721 ctgcctgaaa atgctgacgt gcaacaaaaa tatgcggaca aaatcatcac aaaatttggc
     9781 ttaaaagagt ttttgcaaaa aggttatacc aaagaaatca atttggtatc cagtacgctg
     9841 aataagaaag agcgggtgtt gcacgcttta ttatttgctt ggtatcgcca tcaaatcgcg
     9901 ttgaaatacg gcattgccaa tttcaagcct gtgatgttgt ttagaagtaa gacgattgat
     9961 gaatcaaaag cggattattc ggcattttta aattgggcag aaaatgtaca agcggatgat
    10021 tttgagtttt tagatacgtt ttcaggcagc ctgaataata gcgacaacgc caacgaacaa
    10081 ggcaaaaccc gaaccgaaca agccctaaaa tttatgcagg aaaatggctt tgagtttgca
    10141 catttggcaa attgggtgaa acaaaattat caaaaacata atgtgattat taccaactcc
    10201 gaaaccaaca aaaacaaaac tgaaaaaacc gacagcgaaa ccgaaaaatt gctaaacaat
    10261 ttggaagcgg ctgataatcc aatccgtgcc atttttacgg tagatagatt aaccgaaggt
    10321 tgggacgttc tgaatttgtt tgatattgtg cgtttgtatg aagggcaaaa tggcggtggt
    10381 tcaaataaaa aatcaggcaa aacggctgcc gctaccgtat cggaaaagca gttgattggt
    10441 cgtggcgtgc gttattttcc atttgcgttt gaaggtaaac agccgaataa acgcaaattt
    10501 gacaacgaca tgcaacacga attgcgtatc ttggaagaat tgttttatta cacgcacgat
    10561 gagcaatccc gctatatttc agaactgaaa aatgagttgc gaaaagacgg ttatttgcct
    10621 gaaaaagacg atgataaggt gttggcaaca tttaaactca aatctgaatt tgccgataat
    10681 aaggatttta gagagttgtt aatttgggcg aataaaaaaa tccccaaccc aaacgccaaa
    10741 agcaataatg cagacagcct gcaagccaat ccgcaaacgc tttcattcca agttcacggt
    10801 aatcaactgt tgcaggaaac gcaatttaca gccgatgaaa atgatgaaac agcccgacaa
    10861 atcggcacac aaaataattt tactcaaacc ataaaaatga gtgaaatgga gcggcacatt
    10921 ttcaataaag cattacatat caaagggaaa aatagtcaat ctttattcca tttcaaccgt
    10981 ttgcaaagca agctcgacat tcaaaatcgc aatgaattgc aaaataactt gttgaaagac
    11041 tggcaaattg aatttttagg attagggcaa gacaaacagg ttcgcttgga cgataaactt
    11101 gcaggctgcc tgaaaatctt ggaaatggtt gaaaaacatc tgaatgaaag tgatatgccg
    11161 tttatcggta caaaagaatt tacacctaaa aaattatggg aaattttcgg cacgccaaaa
    11221 caaaaatggg tcaaaaaaga tgatgtaaaa acgactattg ccacgcaaaa tgattggtat
    11281 gtgatgaata attttgccgg aacgggtttg gaagaagcgt taattcaatt tatttcagcg
    11341 cgtttgggcg atttgaaatc taaatatgac gttcatttaa tccgtaatga agaagtgttt
    11401 aaattgaata actttgccga tggtgaagga tttatgccgg actttatttt attgctgaaa
    11461 gataaacaaa aatcttcttc caatggcgtg aaggactttt tgcattacca aattttcatt
    11521 gagcccaaag gtgagcattt agtggaaact gatggatgga aagaagaatt tttagaagcc
    11581 atcactgtgg aatatgggat ggataagatc ctgcaaaaag atacaccgca ttatcgtttg
    11641 atcggtttac cgttttttac tgatcatcag aaaaatggac aatttacgga gtcattccct
    11701 ttaggtgcgg catcgcttga aaaaatgaag tagtgcattg caggcaaccc cgtttgacaa
    11761 aacttccttt acaaaagggc gttttgtcag atatttaatc aacacattat taaaatacag
    11821 ccaaatttta atgccgtccg aaccctgtgt tcagacggca tcgtattttt cagtatctaa
    11881 accgtttccc tgccccaatc tttgcctctc aaaatcgaag catcgacatc ttgaatatcg
    11941 cggtgtcccg taaacgccat agatatatcc atttctttat acaggatttc cagcgcacgg
    12001 gttacgcctt cttcaccata cgcacccaaa ccatacagaa acgcccgacc tatcattgta
    12061 cctttcgcac ccaaagccca cgccttcaaa atatcctgac cgctgcggat gccgctgtcc
    12121 atccaaactt cgatgtcgct gccgactgca ctaacgatgt cgggcaaggc tttgatggcg
    12181 gacacggtat cgtcgagctg gcggccgccg tggttggaaa cgaccaatgc gtccgcaccg
    12241 cttttcgctg ctttttccgc atcttcaggt tccataatgc ctttgataat cagcttgcca
    12301 ccccacaaat ctttaatgcg tgcaacgtcg tcccagctca ggcgcgggtc gaattgttcg
    12361 gaagtccatg aagacagcga ggacaaatcg ccgacgtttt tggcgtgtcc aacgatattg
    12421 cggaacgtgc ggcgttccgt gttcagcatt ttcatacacc attcgggctt ggtcgccaga
    12481 ttgattaaat tggcgatggt cggtttcggc ggcgcggaca ggccgttttt gatgtctttg
    12541 tggcgttgcc ccaaaacctg caaatcagcg gtcaatacca atgccgaaca tttggcatcc
    12601 ttcgcgcgct taatcaggtt ttccataaac tcgcggtcgc gcatcacata aagctgaaac
    12661 caaaatggtg cggaagtgtt ctcggcaacg tcttcaatcg agcagataga catcgtggac
    12721 agcgtaaacg gaatgccgaa cttctccgcc gcccgcgccg ccaaaatttc accgtcggcg
    12781 tgtgccatac ccgtgaaacc cgtcggcgca atcgccaccg gcattttcac atcctgcccg
    12841 atcattttgg tctccaggct tcggccttcc atattgacca ataccttttg tcggaagcgg
    12901 atgtctttga aatccgaagt gttttcacgg taggtagttt ctgtccacga acccgaatcg
    12961 atgtaatcgt aaaacatacg cggcattttg cgcttggcaa cgcggcgcaa gtcttcgatg
    13021 caggtcattt tgctcaaatc acgtttcatt tgtcgccccc tgaatacctg aataacttta
    13081 tatgaaatag ataatgtata tcaatattga ttataaggca aatcatttca acatttactg
    13141 cacccgccgc cgctcccact tcaagcggca taaggtttag aattcaaaaa taatatatta
    13201 aaatcaaaat attaaaaatc aatcaatctg ccgatttaaa cagccaatcg cgcaatccgc
    13261 ccccatactt gactgaaaca ctcagatatt ggacaattcc gcccactata aaaaaagccg
    13321 acacgggcaa ccaccaccat gagactgacc accaaagggc gttttgccgt taccgctatg
    13381 ctggatttgg cgatgaacgc gcaaaccggc gccgtcaaac tcagtgccat cagcgaacgc
    13441 caaaacatat ccctctccta tctcgagcaa ttgttcggca aactccgccg cgccggactt
    13501 gttgaaagcc tgcgcgggcc cggcggcggc tacatcctcg ccgcaccggc ggcacgcatc
    13561 aacatcgccc aaatcatcgc cgccgccgaa gaccggctgg acgcaaccca atgcggcagc
    13621 aaagccaact gccaccacgg cgcgccctgc ctgacgcacg acctttggga gaatttaaac
    13681 aaaaccatca acgactacct cggcagcgtt accctgcaaa gcatcatcga acagaaaaac
    13741 aacggcgacg gcagccgcgt cgtccaattt acacacatcc attaaataac acccgaaaaa
    13801 gaaagagcaa accatgaccg tcaaaacccc cgtttacctc gactacgccg ccaccaccac
    13861 cgttgacaaa cgcgttgccg aaaaaatgat tccctatctg accgaaacct tcggcaaccc
    13921 cgcctccaac agccacgcat tcggctggga agcagaagag gccgtcgaaa aagcccgcgc
    13981 cgacatcgct gccctgatta atgccgaccc caaagaaatc gtcttcacca gcggcgcgac
    14041 cgagtccgac aacctcgcca tcaaaggcgc ggcaaacttc tacaaaacca aaggcaaaca
    14101 cctcattacc gtcaaaaccg aacataaagc cgttttagat acaatgcgcg aactcgaacg
    14161 ccaaggtttt gaagtaacct acctcggcgt tcaagaaaac ggtttgattg atttggaaga
    14221 actcaaagcc gccatccgcg acgacaccat cctcgtttcc gtaatgtggg caaacaacga
    14281 aatcggcgtg gtgcaagaca ttcccgccat cggcgaaatc tgccgcgaac gcaaaatcgt
    14341 cttccacgtc gatgccgcac aagcctgcgg caaagtgcct gtcgatgtcg aagccgccaa
    14401 aatcgacctg ctctcgatgt ccgcgcacaa agtgtacggc cccaaaggca tcggcgcgct
    14461 gtacgtccgc cgcaaacccc gcgtccgcct cgaagcccaa atgcacggcg gcggccacga
    14521 acgcggcttc cgcagcggca cattgccgac ccaccaaatc gtcggtatgg gcgaagcctt
    14581 ccgcatcgcc aaagaagaac tcgaacaaga tatggcgcac taccgcaaac tgcgcgatat
    14641 tttcctcaaa ggtatcgaag gcatcgaaga agtctatatc aacggcgacc tcgaacatcg
    14701 cgtcccgaac aacctaaacg tcagcttcaa cttcgtcgaa ggcgaaagcc tgattatggc
    14761 agtgaaagaa ctcgccgtat ccagcggctc cgcctgcacc tccgcctcgc tcgaacccag
    14821 ctacgtcctg cgcgcattgg gcagaaatga cgaattggcg cactcgtccc tgcgcatcac
    14881 cttcggtcgc atgaccactg aagaagaagt gcaatttgcc gccgaactga ttaaatccaa
    14941 aatcggcaaa ctgcgcgaac tgtcgccgtt gtgggaaatg ttcaaagacg ggattgattt
    15001 gaactcgatt gaatgggcgg cgcattaaag cgtaccaaca tgccatccga accttcagac
    15061 ggcattccaa aaacaaagca atcaagagaa aaatatgaac gaacaagatt tagatttgga
    15121 caacctcgac aacctgcttg acgatttcga cggcgttacc gtggaaggcg gcgtcgattc
    15181 ggaaaacgac gacggctgtg aaggcggagc gtgcaaaatc taactcaggc cgtctgaaaa
    15241 ccggcaaaca aaccacttaa atcatcaaaa cattaaggaa accacatcat ggcatacagc
    15301 gataaagtaa tcgaccacta cgaaaatccc cgcaacgtcg gtactttcga caaaaacgac
    15361 gaatctgtcg gcaccggcat ggtcggcgca ccggcctgcg gcgacgtgat gcgcctgcaa
    15421 atcaaagtga acgatgaagg catcatcgaa gatgcgaaat tcaaaactta cggctgcggt
    15481 tccgccatcg cttcgtccag cctgattacc gagtgggtta aaggcaaaag cctggatgac
    15541 gcgctggcaa tcaaaaacag cgaaatcgcc gaggagttgg aattgccgcc ggtaaaaatc
    15601 cactgctcca tcttggctga agatgcggta aaagcggccg ttgccgacta ccgcaaacgt
    15661 caggaaaaca gataaagccc ttcagacggc atcgtcccgc aatgccgtcc gaaccacccg
    15721 ccgcttcagg tccgtccggg gcggtacaac aaggaagaaa tatgattacc cttaccgaga
    15781 atgccgcaaa acacatcaat gactatctcg ccaaacgcgg caaaggcttg ggcgtacgct
    15841 tgggtgtgaa aaccagcggc tgctcgggga tggcgtacaa ccttgaattt gtcgacgaag
    15901 ccgatggcga cgacctgatt ttcgaaggac acggcgcgcg catttatatc gatccgaaaa
    15961 gcctggttta tctggatggc acgcaagtcg attacaccaa agaaggtttg caggaaggtt
    16021 tcaaatttga aaaccccaat gtcaaagact cctgcggctg cggcgaaagc ttccatgttt
    16081 aagacataaa aatggcggga ccgtatcaaa accgtcccgc cattttttcg cttcctgcct
    16141 gttgtagctg cctttgcctt ttcttttccg ttccaccttg tgtcggaaca aatcagattt
    16201 cactaaggct tttaaagcat tgtcgcgtat tttgccttta ttgtgctgca ctttgccgcc
    16261 catattcagt cctttcgttt aagaagcggc agattataag gcaaaaacag ttttctgcca
    16321 aaatcttaca tttatcatcc tactatgtcc caatatttca ccctcttccg gattgaaccc
    16381 gctttcgata tcgacaccga aaacttggaa caaacctacc gcgccttggc cgcccgtttc
    16441 catcccgata aattcgcttc agcttccgcc tttgagcaaa agcaggcagt gatgatgtct
    16501 tccaccatca acgatgccta ccgcaccttg aaaaacccca tcgaccgcgc cgcctacctg
    16561 ctgaaaacat cgggcatcga tgccgacgcg ccggagcata ccgctttcgc ccccgaattc
    16621 cttatgcagc aaatggaatg gcgcgaaacg ctgatggagg cacgggcagg caacgacctt
    16681 gaatccttga aaaatctcga caacgaaatc cgcgacgaac aagaaaaact gttctgcggt
    16741 ctgaaacagt cgtttgcccg acaagattac gacacagccg cacaacaagt ccgccaaggc
    16801 aggtttctcg acaaactccg caacgaaatt tcctcggcat tataatccgc accgtgtttc
    16861 agacggcgta accgccgcac cgttccgcgt caaaatatgc taaaataagc aacaattttt
    16921 tgccatacga aacattgaaa ccatgaccga cgcaaccatc cgccacgacc acaaattcgc
    16981 cctcgaaacc ctgccggtaa gccttgaaga cgaaatgcgc aaaagctatc tcgactacgc
    17041 catgagcgtc attgtcgggc gcgcgctgcc ggacgttcgc gacggtctca agccggtaca
    17101 ccgccgcgta ctgtacgcga tgcacgagct gaaaaacaac tggaatgccg cctacaaaaa
    17161 atcggcgcgc attgtcggcg acgtcatcgg taaataccac ccccacggcg ataccgccgt
    17221 atacgacacc atcgtccgta tggcgcaaaa tttcgctatg cgttatgtgc tgatagacgg
    17281 acagggcaac ttcggatcgg tggacgggct tgccgccgca gccatgcgct acaccgaaat
    17341 ccgcatggcg aaaatttccc acgaaatgct ggcagacatt gaggaagaaa ccgtcaattt
    17401 cggcccgaac tacgacggta gcgaacacga gccgcttgta ctgccgaccc gtttccccac
    17461 actgctcgtc aacggctcgt ccggcatcgc cgtcggcatg gcgaccaata tcccgccgca
    17521 caacctttct gataccgtca atgcctgcct gcgcctgctc gatgcacccg acaccgaaat
    17581 cgacgaactg atcgacatta tccaagcccc cgacttcccg accggggcaa ccatctacgg
    17641 cttgagcggc gtgcgcgaag gctataaaac aggccgcggc cgcgtcgtta tgcgcggtaa
    17701 gacccatatc gaacccatag gcagaaacgg cgaacgcgaa gccatcgtta tcgacgaaat
    17761 cccctatcag gtcaacaaag ccaagctggt cgagaaaatc ggcgatttgg ttcgagaaaa
    17821 aacactggaa ggcatttccg agctccgcga cgaatccgac aaatccggta tgcgcgtcgt
    17881 tatcgagctg aaacgcaacg aaaatgccga agtcgtctta aaccaactct acaaactgac
    17941 tccgctgcaa gacagtttcg gcatcaatat ggtggttttg gtcgacggac aaccgcgcct
    18001 gttgaacctg aaacagattc tctccgaatt cctgcgccac cgccgcgaag tcgttacccg
    18061 acgtacgctt ttccggctga agaaggcacg ccatgaaggg catatcgccg aaggcaaagc
    18121 ggtcgcactg tccaatatcg atgaaatcat caagctcatc aaagaatcgc ccaacgcagc
    18181 cgaggccaaa gacaaactgc ttgcgcgtcc ttggcgcagc agcctcgttg aagaaatgct
    18241 gacgcgttcc ggtctggatt tggaaatgat gcgtccggaa ggattggctg caaacatcgg
    18301 cttgaaagag caaggttatt acctgagcga gattcaggca gatgctattt tacgcatgag
    18361 cctgcgaaac ctgaccggcc tcgatcaaga agaaattgtc gaaagctaca aaaacctgat
    18421 gggtaaaatc atcgactttg tggatatcct ctccaaaccc gaacgcatta cccaaatcat
    18481 ccgcgacgaa ctggaagaaa tcaaaaccaa ctatggcgac gaacgccgca gcgaaatcaa
    18541 cccgttcggt ggcgacattg ccgatgaaga cctgattccg caacgcgaaa tggtcgttac
    18601 cctgacacat ggcggctata tcaaaaccca gccgaccacc gactatcagg cgcagcgtcg
    18661 cggcgggcgc ggcaaacagg cggctgccac caaagacgaa gactttatcg aaaccctgtt
    18721 tgttgccaac acgcatgatt atttgatgtg ctttaccaat ttgggcaagt gtcattggat
    18781 taaggtttac aaactgcccg aaggcggacg caacagccgc ggccgtccga ttaacaacgt
    18841 catccagttg gaagaaggcg aaaaagtcag cgcgattctg gcagtacgcg agttccccga
    18901 agaccaatac gtcttcttcg ccaccgcgca gggaatggtg aaaaaagtcc aactttccgc
    18961 ctttaaaaac gtccgcgccc aaggcattaa agccatcgcg ctcaaagaag gcgactacct
    19021 cgtcggcgct gcgcaaacag gcggtgcgga cgacatcatg ctgttctcca acttaggtaa
    19081 agccatccgc ttcaacgaat actgggaaaa atccggcaac gacgaagcgg aagatgccga
    19141 catcgaaacc gaaatttcag acggcatcga agatgaaacc gccgacagcg aaaacgcact
    19201 gccgagcggc aaacacggtg ttcgcccgtc cggtcgcggc agcggcggtt tgcgcggtat
    19261 gcgcctgcct gccgacggca aaatcgtcag cctgattacc ttcgcccctg aaaccgaaga
    19321 aagcggtttg caagttttaa ccgccaccgc caacggatac ggaaaacgca ccccgattgc
    19381 cgattacagc cgcaaaaaca aaggcgggca aggcaatatt gccattaaca ccggcgagcg
    19441 aaacggcgat ttggtcgccg caaccttggt cggcgaaacc gacgatttga tgctgattac
    19501 cagcggcggc gtacttatcc gcaccaaagt cgaacaaatc cgcgaaaccg gccgcgccgc
    19561 agcaggcgtg aaactgatta acttggacga aggcgaaacc ttggtatcgc tggaacgtgt
    19621 tgccgaagac gaatccgaac tctccgacgc ttctgtaatt tccaatgtaa ccgaaccgga
    19681 agtcgagaac tgaaaatcat ctcccgatgc cgtctgaaga ttcagacggc atttatttta
    19741 tccctcatcc gtcatccagc ttctcacaat atagcggatt atagtcaatt aaaaacaagg
    19801 ggctgtccta gataactagg gaaattcaaa ttaagttaga gttgccccta tgagaaaaag
    19861 tcgtctaagc cggtataaac aaaataaact cattgaactg tttgtcgcag gtgtaactgc
    19921 aagaacggca gcagagttag tagg
//
LOCUS       DAWFKN010000031        19642 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFKN010000031 DAWFKN010000000
VERSION     DAWFKN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.031"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(49..1218)
                     /locus_tag="WGR46_001103"
     CDS             complement(49..1218)
                     /locus_tag="WGR46_001103"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0856054.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1401..1511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1564..2460
                     /locus_tag="WGR46_001104"
     CDS             1564..2460
                     /locus_tag="WGR46_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0856055.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WGR46_001105"
     CDS             complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WGR46_001105"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0856056.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3684..4871)
                     /gene="ccsB"
                     /locus_tag="WGR46_001106"
     CDS             complement(3684..4871)
                     /gene="ccsB"
                     /locus_tag="WGR46_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0856057.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4864..6816)
                     /locus_tag="WGR46_001107"
     CDS             complement(4864..6816)
                     /locus_tag="WGR46_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0856058.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINRKDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGDGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7093..7716)
                     /locus_tag="WGR46_001108"
     CDS             complement(7093..7716)
                     /locus_tag="WGR46_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0856059.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7922..8551
                     /locus_tag="WGR46_001109"
     CDS             7922..8551
                     /locus_tag="WGR46_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0856060.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8672..11068)
                     /locus_tag="WGR46_001110"
     CDS             complement(8672..11068)
                     /locus_tag="WGR46_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0856061.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11220..12335
                     /locus_tag="WGR46_001111"
     CDS             11220..12335
                     /locus_tag="WGR46_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0856062.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12338..12937
                     /locus_tag="WGR46_001112"
     CDS             12338..12937
                     /locus_tag="WGR46_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0856063.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12938..13585
                     /locus_tag="WGR46_001113"
     CDS             12938..13585
                     /locus_tag="WGR46_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0856064.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13603..14148
                     /locus_tag="WGR46_001114"
     CDS             13603..14148
                     /locus_tag="WGR46_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0856065.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14167..16452
                     /locus_tag="WGR46_001115"
     CDS             14167..16452
                     /locus_tag="WGR46_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0856066.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQTAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17630..18142
                     /locus_tag="WGR46_001116"
     CDS             17630..18142
                     /locus_tag="WGR46_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0856067.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18222..19301
                     /gene="aroB"
                     /locus_tag="WGR46_001117"
     CDS             18222..19301
                     /gene="aroB"
                     /locus_tag="WGR46_001117"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0856068.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5244 a         5606 c         4508 g         4284 t
ORIGIN      
        1 aatgccgtct gaactgttca gacggcattt ttgattttca aaccggaatt acagccccgc
       61 tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa gtgaactcag gttcttcgcg
      121 gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg cgcaagagat cgagcatttg
      181 gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact aaggtaatca gtttttcttc
      241 gctgattttg cctgtgccga aagtatcgat ggaaatcgaa gtcggttcgg caacgccgat
      301 ggcgtaggaa acttgaattt ggcattgggt tgccaagcct gcggcgacga tgtttttcgc
      361 cacataacgg caggcgtagg cggcggaacg atccactttg gacgggtctt tgccagagaa
      421 tgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatcg acgatgattt tacggccggt
      481 caaaccgcag tcgccttgcg ggccgccgat gacgaaacgg ccggtcgggt tgatcaggta
      541 tttggtttcg tcggtcagca gttcagacgg caaaacgggc ttgataatct gttcgatgac
      601 ggcgtttttc agctcttcat aaccgatttc cgggtcatgt tgcgtagaga ggacgactgt
      661 atcaatgcgt tttaccttac cggtttcgct gtcgtaaacc acggtcaact gcgcttttgc
      721 gtcgggacgc agccacggca ggcggccgtc tttacgcaat tcgctttggc gttgcatcag
      781 gcggtggctg taatagatgg caaacggcat cagggtcggg gtttcgtcgc aggcataacc
      841 gaacatcaaa ccttggtcgc ctgcgccttg gttcaagtcg ataccttcgc cttcgtttac
      901 gccttgggcg atgtcggggg attgttggtc gtagtacacg ccgaccgcgc agccgttggc
      961 atcaaagccc agctcggagg agttgtagcc gatgcgtttg atggtttcgc gtgcgacttt
     1021 gatgtagtcc acttgggcgg tggtggtgat ttcgcctgcc aatacgcaca agcctgtgtt
     1081 gaccaaagtt tctgcggcga cacgcgcttt ggggtcttgc gccaagatgg catccaaaat
     1141 cgcatcggat acttggtcgg caactttgtc cggatggcct tcggataccg attcggaagt
     1201 aaacagatat tcgctcattg agattttcct tgaaaaacaa accatcttct tcagacggca
     1261 tgttgtatga acataatgtc gacagcggga aatatagcaa aatttcccta ttcataccat
     1321 tcagttgaga aatattccca tttgaatggc actttggaat ctctgcccgt acgtttctta
     1381 caggcaaaaa aattcccgca tcaagcgggt ttggattgct ctggtgagcc acatcggctt
     1441 ttcaaccgtc caccttactt ttccttttga aaagcaggtt ggcatggaat tcccaactct
     1501 taatgcagcg cggatggtag cagaaaagcc tttccgccgc aatacttccc tttttcagac
     1561 ggcatgtttc gtttacaatt cgggctgttt ccccctttgc gaaccgccat gcacatcctg
     1621 ttgaccgccc tgctcaaatg cctctccctg ctgccacttt cctgtctgca cacgctggga
     1681 aaccggctcg gacatctggc gttttacctt ttaaaggaag accgcgcgcg catcgtcgcc
     1741 aatatgcgtc aggcaggcat gaatcccgac cccaaaacag tcaaagccgt ttttgcggaa
     1801 acggcaaaag gcggtttgga acttgccccc gcgtttttca gaaaaccgga agacatagaa
     1861 acaatgttca aagcggtaca cggctgggaa catgtgcagc aggctttgga caaacacgaa
     1921 gggctgctat tcatcacgcc gcacatcggc agctacgatt tgggcggacg ctacatcagc
     1981 cagcagcttc cgttcccgct gaccgccatg tacaaaccgc cgaaaatcaa agcgatagac
     2041 aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa ccgcgcctac cagcatacaa
     2101 ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag caaccatcgt cctgcccgac
     2161 cacgtcccct cccctcaaga aggcggggaa ggcgtatggg tggatttctt cggcaaacct
     2221 gcctatacca tgacgctggc ggcaaaattg gcacacgtca aaggcgtgaa aaccctgttt
     2281 ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt tgcacatccg ccccgtccaa
     2341 ggggaattga acggcgacaa agcccatgat gccgccgtgt tcaaccgcaa tgccgaatat
     2401 tggatacgcc gttttccgac gcagtatctg tttatgtaca accgctacaa aatgccgtaa
     2461 cgaaaataaa aatgccgtct gaacaatttc agacggcatt ttgtcatctg acgatttccg
     2521 acagcggcca gcgcggacgg acattgaacg caccgacctc cctgcccttg cctaggtgca
     2581 tcgcaccggc aaaggcaatc atggcaccgt tgtccgtgca atatgccgtc ggcgggaaaa
     2641 acacgctgac tttttcggac ggatgtttcg gcttgccttt gggggtcggg atttgcaccg
     2701 tcatattgcc gaaagtttca cggagcttgc ggtttgcacc gaccccgccg gcgaccacta
     2761 cggttctgaa ccctgtctgc aacagggctt ttttcacttt cgccgccaac acatcgacta
     2821 ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg ctcaggaatg tcatccgccc
     2881 cgttttccgc gcgcactttc tcgacggcgg tcaatacggc ggttttcaaa cctgaaaaac
     2941 tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa aacaaacgct tcgggcctgc
     3001 ccgattccgc aagttccgac agtttcgcac cgcccggata cggcaagccc agcagtttcg
     3061 ccgttttgtc gaatgcctcg cccgccgcat catcgacgct ctcgcccaaa agcgcgtagt
     3121 cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc cgaaaccaac agcgcgacaa
     3181 aaggaaagtc gggtttttcc tccgccaaca gcggcgacag cagatgtcct tccaaatgat
     3241 ggacggggat gacgggcttg cccaatgcca aagccagtgc attggcgtag ctcgaacccg
     3301 ccagcagcgc accgcccaaa ccaggcccct gcgtaaaggc aaccgcgtca atgtcgccat
     3361 acgatgcgcc tgcctgcgcc aaacagcctt cagtcaacgg aacaaggcgg cggatatggt
     3421 cgcggcttgc caattccggc acaaccccac cgtattcggc gtgcattgcc atttgagtgt
     3481 gcaggcagtg cgctcgcaat cctcgttccg tatcgtaaag cgcaacgccg gtttcgtcgc
     3541 aagaagactc gattcctaat accaacatgg tctgatgccg ttaaaaactg aaaaacgtat
     3601 tttagcggat ttcggcacga ctgccgtatc ccaaaaacgg aacatgccgt ctgaagccct
     3661 tcagacggca tcgtcgcacc gtatcaaagc gttccgtaag aatgcagccc gctcaaaaac
     3721 atattcacgc cgataaaagc aaatgcggtt acgaacaaac cgataatcgc ccaccacgcc
     3781 agcactttgc cgcgccaacc ggcaaccagc cgcaagtgca gccaaacggc gtaattgagc
     3841 cagacgatga acgcccacgt ctctttcgga tcccaactcc aatagcgtcc ccaagcatct
     3901 gccgcccaca gcgcacccaa aatggtggca atggtaaaga acagaaagcc gacggcaatc
     3961 gccttataca tcacctcgtc gatcaatgcc gacggcggca gccacagttt tccgcctttt
     4021 ccttccgcac gcagggaaac cagttcggca ataccgagca tcgcggaaat gcaaaacgcg
     4081 ccgtaaccga taaagtttgc cggaacgtgg attttcatcc accaagactg gagcgcggga
     4141 atcagcggct ggatggtatg cgcctcgcgg gacacgctgt accacaagac aaatccaacc
     4201 acgaccgcca taaagccgaa cacgaagccg cccaatttct gtatggcgaa cttaccttca
     4261 taataaagat acatcagcgc ggtaatcacc aaaaacagga tgaacacttc ataaaggttg
     4321 gaaaccggaa tatgccccgc atcgggacgg agcagatagc tttcgtgcca acgtaccagc
     4381 agaccggtaa agcctgctac ggcagacacc catgcaaaca cggttcccat acccaacagc
     4441 gtgttggtcg gcacattttt tacgcttgcc aaaaccgcgc ccgaaatata ggcgaacagg
     4501 gcgaaaaaga caaaggcgca ctgccacata atcgccgact ggctgctgag gaaataccgc
     4561 aacaggaaaa tctctgccga tttgatgttg ccgccataca gaccgacggc ggcataggca
     4621 agcaatacgc ttaaaggaac aaaccagcgc atcggtttga aaaaccaacc caaaaacacg
     4681 gcaataccgg cactcgccca caacatgacc gtttcgtaaa tgtccatatg cataccggaa
     4741 cgggtctgta cgaaaaccgt agccgcaaaa accagcacgg caaataccca atcccaaaga
     4801 ttcagattgc tgatcaaaga cttctgaatc agcagctcgt gttccggaag ggttttatag
     4861 tgttcagtca tgattcaagt ccttgccgag ccgttgcaga ctctcgacgt gttttggaaa
     4921 ttccttctgc aaatcccgtt cgctgcgggc cgaagacatg gcaaaacgga ttttgccgtc
     4981 tgaaaacaat acccacgccc gtttttcgcg cacataaaac atcaataccg tacccaatac
     5041 caacagcacc gagccgagat agaccaaaag cgcacccggg gaacgggtca tctgcaaacc
     5101 cgacgaacgc acctcggaaa acccgtcaag ctggagcagc atgggcgcgg gatattcggt
     5161 caaacccgtg tacgcatcca tactgtgcag caggaaacgg ttccgcgctt catcctgctg
     5221 ccattccggc aagccgtacc ggcgtatggt ttcatccaaa gcagcgttca tcacgccgta
     5281 aagcatttcg tagaaatagc cctgcatctt atcctgctgc tctttcggga tattggacgt
     5341 aataaattcg tccaatccca aatagccttt ttgtgcaaag atgttcagcg tgttttccgc
     5401 agccagcatg aattgttcgc ggatttcggc aggtgcgcct ttggttgcgt cggcaaccag
     5461 acgtttgcgc ccgtccccat ctttcaaaaa ctcacgcaac gccataaagg tgtccgcttt
     5521 caactgcttg tccaagggga tacgcagcca gcggtattgc tgctgcaagc cgctgcgcgt
     5581 gccggtaatc caaaaataat cctgttcctg caaaaccggc agcatatagt ttttatattc
     5641 gaccgcctgc cctgccgcat cacggatacg gtaaacaatg gaagggccga tattggtgta
     5701 ttttttacct tcctgagtaa cggcgcggac atcgttcagc gtggatttca ggcttttttc
     5761 ccgttccgcg ccctcgctca tgtcctccac attcatagaa gtgaactgat cgaactcaag
     5821 acgatatttg tgtttgccaa tttccaacgg aaactggtgt atggatgttg ccttcaacac
     5881 gacaggctcg cgcgaagcat cacccaaatt ccacgccttg aatgtcaaat ccgaaccgcc
     5941 gtcggcaaaa ctcgcctgat aaatcgtgat gccgtgcaag gtcaaaggat ggttcacgcg
     6001 gatggtgcgc tcgagtttct caccggttgc cttgtccgtc acttcaatat cgctggcgaa
     6061 atcgcgcggc ataccggtat tgtaaaaatc gatatggaat tttttcagtt tgacttcaaa
     6121 aggcaagtcc tgaaccaata tcccgttgtc ggcattcagg aaaaccacat ccgcactctg
     6181 cccctcggaa atattgacgt tgcccctaaa tgagagattg gacgcaccca aaatactttc
     6241 gggcttgaaa tccttggcat aaaccgcctg attgtccgga acaatccgac cggtcagcat
     6301 acccagtttc aacagcaggt tactgtctat caacccgccc aggcaaatga caatcaaagc
     6361 aacatgggca aagatatagc cccatttgtt cattgtgcct tttttggcgg caatcagaac
     6421 cgacccgtct ttacggttaa tggtttttcc ctgaaaacct tgtacttcca gataacgttt
     6481 ggcaacctcg ggcgcaattt ttacatccaa cagcgaagaa tggcgcatcg ccgccagaga
     6541 tttttcttta accttttccc gaaaagactt catttcgcgc cagaacggcg gcacattgcg
     6601 aatcaggcac aaactggtag aaaccaccaa aaacatcatg ataacgacaa accatgccga
     6661 agcatagacg tcatacagtc ccagaaaacc aaaaatctgc gcccaaaaag gtccgaattt
     6721 gaccaaataa tccgtctgcg gctggttttg ctgcaacacc gtaccgataa ccgatgcaat
     6781 acccagcaga ctgagcaaag cgaccgcaaa gcgcatggag ctgaaaaaag cgaaccacgg
     6841 acgggaaaga agtgggggag atctacggga tttactcatt gtgtgtttat tccgccatca
     6901 ggaatatggg aaagcagaat tgggcaaaca gaaaacaacg tcccgattct actgtcttga
     6961 tgctttttat ttcaagacaa tgaagacagc ctgcatcgat tccaacggtt gcgattgaaa
     7021 aaacttatcg cagaattgcc tgaagccgtc tgaaaacttt tcagacggcc tctaaaacag
     7081 actattgcgg aattaacgca aaccttggat aaagttggcg accgctttca aatcttcttc
     7141 agacatacgg tttgcaatat cttccatgat ggtattttta cgctgaccgg acttgtaggc
     7201 attcatctgt tcaacaatat atgcctgatg ctgaccgccc aaacgcggat aagcctgaat
     7261 ttcgcttccg cctcccggca tacccgcacc gctcggaccg tggcaggaca tacacgccgg
     7321 cactttttta tcgctcaaac cgccgcgata gattttcgca cccaattcgg gattttcctt
     7381 aggattggct tcaccggatt tgggctgctg tttggcatag aatgcggata cgttcaaaat
     7441 atcctgatcg ctcaaattca ttaccaccgg tttcatcaca gctgccgaac cgtgggtgcg
     7501 tttaccgtcg cggatgccga tggtttgatg atagatgtaa gcagtatgct gtgccgccaa
     7561 acgcggatac atcgcaatgc cgctgttacc gtctgctgca tggcaagccg cacaaaccgt
     7621 tgcggcaacc tgtttgcctt tttccacgtc tgctttggga gatgcggaaa ccgcaccggc
     7681 agccaaaaca aaggccaata aagtcaatcg tttcatggag tgctcctgat tacagcattg
     7741 gataacgcaa caatgctctt tttatattca aatacgggat ttttgacccg attaaaaccg
     7801 atgattctgc aaacgtgtta ttctatacta aatttacatt aaattaccac tgtgtttcac
     7861 ataaaaccaa ccgcatattt ttgctgtcgg acaaacggcg gcggaaaaca aggatatgcc
     7921 catgaacctt tttcaaaacg ccaaattctt cacgacggta aaccatctca aagacctgcc
     7981 ggacacaccg ctcgaaattg cctttgtcgg caggagcaat gccggaaaat ccagtgccat
     8041 caataccctg accaaccatg tccgtcttgc ctacgtttca aaaacacccg gacggacgca
     8101 gcatatcaac ttcttcgagc tgcagaacgg caattttatg gtcgatttgc ccggctacgg
     8161 ttatgcccaa gtccccgaag cagtacgcgc acattgggtc aatctgctcg gcgactatct
     8221 gcaacagcgc aaacagctta tcgggctggt tttgattatg gatgcccgcc atcctttaaa
     8281 agaactcgac atccgtatgc tggacttttt ccacacgacc ggcagaccgg ttcacatcct
     8341 gctgtcaaaa gccgacaaat tatccaaaaa cgaacagata aaaaccctgt cccaagtcaa
     8401 aaaactgctc aaaccttatt ccgacaggca aaacatcagc gtacagctgt tttccagcct
     8461 gaaaaaacaa ggtattgacg aggccaaccg aactgtcgga agctggtttg acgcagcaga
     8521 tgccgccgct tcctctccaa aggaaaactg accccaatta tacggaaacc gtattccccc
     8581 cacttgaccg accgcaaaca tttaaaaaat tgccactgcc aaatctaaaa tgccgtctga
     8641 aagacttttc agacggcatt ttgcggagtc tttaaaacag agaatccaac tgctgctgtt
     8701 tggaaccagt attactcgga agcaccggcg tttcctgcat atcttggcgg acttcgtcat
     8761 ccgccgcctg ccgtccgcct tctgccgcgc ccccgtcatc ttcttttgcc cgtcgggaag
     8821 gttgcggcgc aataccgctg ttgtccagcg tcaagcccgg atcggttacc atacgttcct
     8881 tcatatagta ttcgccattg ctgctgacca caccttcagg cattttcatc cccttgccct
     8941 gctttccttt caacgcaaaa cgcatatagt ccacccaaac cggcaccgca atcgtaccgc
     9001 cgtagccgac acgccccata ctcttaggtt tgtcgaagcc gatatatacg gcagtaacca
     9061 catcagggtt aaaaccgaca aaccacgcat ccttattgtc attggtcgta cccgttttac
     9121 cggcaatatc cgttcttccc aacgcagctg ccccccttgc cgtaccaaca cggaccacat
     9181 cctgcataat cttatacata atataggcat tgcgcggatc gattgcctga ggcgcatttt
     9241 gcccggccac caaaggttgc atttgggcgc gcaacctgcc gtctctgtca taaatcttat
     9301 cgattacgtg cgaagaaacc ctatatccgc cgttcgcaaa tacgctatat gcctccgcca
     9361 ctttcaacgg cgttgtctcg cccgtaccta aagccataga caggcttgcc ggcagctcgg
     9421 acgacctgaa gccgaaacgc cggatatact gttgcgcgta accgacaccg atagacatca
     9481 aaatacggat ggaaaccata ttcttggaag ccgtcagagc ctgtctcaaa gtaatgtagc
     9541 cggaatatct gccgtctgaa tttttaggtg tccaaaccga accgttcggc cctttccccg
     9601 gcagggaaat cggcgcatcg ttaaccactg tggacgcggt catcccctta gataatgccg
     9661 ccgaatagac aaacggctta aaggtcgaac ccggctgccg cattgcctga acggcacgat
     9721 tgaatgtttt gctgtgaaaa tcataaccgc cgaccagcgc gcgcacagct ccggtttttg
     9781 catccagcga aaccaaagcc ccctgcagca acggctcttg aaccaccgcc caacgcccgc
     9841 cgttgttttt gacacggatg accgcgcccc tgcggatacg gtcctccccc attttttcat
     9901 tattgaccgc gcgggccgca aaacccaagg cgcgcctgtc aagcgtaacc cgcctgccgc
     9961 cgggcagctg tatgacgaca tttttctttt tagtcacatc caacacaacg gcgggaacca
    10021 ttttatcgac ggtatagagt cccgacagat actggctgac agtctcctcg acatcttcac
    10081 tcttactcaa atcgatatag ttttccgcac cgcggtagct gctgccgcga tcgaaattcc
    10141 gtagagcctt gcgcaatgcc tcggttgcca ccttctgatg atcggcgcgg accgtggtat
    10201 aaaccttaaa accctgcgta taggcatctt caccgtattt ctcatacagt tcctgacgca
    10261 ccatttccgc cacatataaa gcactctgat cgattttccg aacaaaccgc tcgtaatgca
    10321 gttcctcatt caacgcctga tcgcgctgtt gcacggtaat catcttctcc tcgagcatat
    10381 tgttcaaaat atacttctgg cgcaacttgg cacgttctgg attaacaatc ggattatagg
    10441 cagacggagc cttgggcagt cccgcaagca tggcggcttc cgccaaagtc aaatctcgga
    10501 cattcttatt gaaatagatt tgcgcggcag atgcaaaacc ataggcgcgc tgaccgaggt
    10561 aaatctgatt gaaatacaac tcgaggattt tgtctttgct taaagactgc tcgattttat
    10621 aggcaagcaa cacctcattg aatttgcgtg tgaacgtttt ttcactgctc aaataaaaat
    10681 ttttcgccac ctgctgcgta atcgtactcg cacccgactg cacgctgccg gacacgacat
    10741 tgccgacggc agcgcgggca acaccccaaa catccacccc ccaatgccgg taaaagcgtt
    10801 tatcctcggc ggcgataacc gcattccgca acacctctgg gaaatcgccg atttttgtaa
    10861 attcgcgccg ctgctcccca tacataccga tgacttcccc atccgccgaa taaatagtca
    10921 acggcatttt aggctggtaa tgctgcaaag aatccaaaga cggcagtttc ggatacgtta
    10981 ccaaaatagc aatggcaacc aaacccactc caaatacaca aaacccaaaa accaaaccaa
    11041 aacaagtcgt taaaatcttt ttaatcatag ctgaataata atttaccatt attggtatta
    11101 aataaagtaa aatagcaacc gatttctaca aagcacggtt tcaatgtgca aagaacaagg
    11161 aatccattac ggataccgaa acggttactc actgtacaaa taaagcagga actttcatca
    11221 tgcgcttgtt taaaagcttg aaaaacccta aaaaaacaga tgccaagctc cctaaaaaat
    11281 cttcgggact caataaccgc gcggcaatcg gcatcgatat cgaccagcat tccatcaaaa
    11341 tggtccaatt gtcaggacgt agtttaaacc aaattcaatt ggaaaaatac gtcattgcca
    11401 aattaccaaa gaatatcatt caaggcaata aagtccaaaa ttacgatcaa cttgttacat
    11461 atttgcaaca agcctatgcc aaactgggta cttcgtgcaa aaacatcatc gcgtccgtcc
    11521 cgcaaaatct ggcaaccatc gaacaattga cctacacaga caaagatgca gaattagacc
    11581 tgcaggggtt cgtggagtcc tccatctccg aagtcagctc gatatcgctc gaagaagcca
    11641 attacgacta tcaggtcttg tcccaatcgg ccgccggcga agctgtgttg gccgtcgcat
    11701 cgagaaagga tgaaatcgaa cccctgattg acgcattcaa cgccgccggt atgaaattat
    11761 ccgcgcttga tgtggacatt ttcggacaat acaacgccta cgcgctatgg ataaaccatt
    11821 tcgcccccga gcttgcagcc gaaaaagtcg ccattttcgg cgtatatgcc gcacagacct
    11881 acgccttggt catccaagac ggaaaaatcc tatacaaaca ggaaacctcc gtcagcgaag
    11941 aacagctcaa ccaactcatc cagcgcacct atcaggtaac agaagaaaaa gcggaagaaa
    12001 tcatcaactc cccgcaaaaa ccttccgatt accaagaaag cgtggcaaac tatttcaacc
    12061 agcagattac ccaagaaata caaagggtct tgcagtttta ttacaccacg cagaccgcag
    12121 acgatatgac cgacatcaag catatcctgc tgaccgggga agcggcgcgc caggaaggca
    12181 tcgcccaaac cgtcgcctca caaaccaatg cagatgtaca atgcgtccat cccgcgcgtt
    12241 attttgcgga caacctcaaa acagacaaac aacaattcga acttgatgcg ccgacactga
    12301 ccagggcgtt cggtttggcg gtacggggat tataattatg aacaatttaa tcaaaatcaa
    12361 cctcctcccc tacagggaag agatgaacaa gcgcaaacag cagcagttta aaacgctgat
    12421 gtacggtgcc gtgctgacgg gcgttgccgc cgttgcggca acctacctgt ttatcgacaa
    12481 tatgatcaat aaccagtcgg aaagaaacac gctgctggaa acctccatcg cacacttgga
    12541 taccgagctg tcggaaatac aaaagctcaa acaggaaaaa gatgccttcc tgattaagaa
    12601 aaacaaaatc gaggagctcc agctcaaacg cctccaagcc gcaaaaatcc tcgacagcct
    12661 gaatgaggcc gtccccggaa gcacctacct gacctcgctg gatgccgtta ccgccgactc
    12721 ttatcggctc agcggcagga catccagcga caaccgcgtt gccgccatga tgagggcgat
    12781 gcccaatacc ggcatattca agcaacccga attgttaagc atcaagaaaa acaattcgca
    12841 tcaagaattt acccttcagg caacattaca acccatcgta aaggcggccg aatccaaaga
    12901 gaatccggct tcgggaaacg cacaggaggc aaactgaatg gcttctaaat catctaaaac
    12961 caacttggat ctcaacaacc ttcacctgct caaccttcct gccaggcttt ttatcgccct
    13021 gctggccgtt gccgccgtgc tggggctcgg ttatgccgga ttgttcaaaa gccagatgga
    13081 atcccttgag gaatacgaag caaaagaaac cgaactgaaa aacacctaca aacagaaaag
    13141 tatcgacgcg gccagcctga acaacctgag ggacgaactt gcctcaatcc gctctgcctt
    13201 cgatatcatg ttgaaacagc tgccgacaga tgcagaaatt cccaacctgg ttcaagagct
    13261 tcatcaggca ggttcgagca acggcctgcg cttggacagc gttatgcccc aacctcccgt
    13321 agatgacggc cccatcaaaa gattacccta ttccatttcc attaccggaa attacgaaca
    13381 gatcagccaa tttacccgcg atgtcggcag cctctcccga atcattaccc ttgagtcgct
    13441 gaaaatcgcc caatctccgg aaaacggcgg caatcctgac ggcaagagca gcatcctgaa
    13501 cctcagtgcc attgccacca cctaccaagc aaaatccgta gaagagcttg ccgcagaagc
    13561 ggcacaaaat gccgagcaaa aataacttac gttagggaaa ccatgaaaca ctatgcctta
    13621 ctcatcagct ttctggctct ctccgcgtgt tcccaaggtt ctgaggacct aaacgaatgg
    13681 atggcacaaa cgcgacgcga agccaaagca gaaatcatac ctttccaagc acctaccctg
    13741 ccggttgcgc cggtatacag cccgccacag cttacagggc cgaacgcatt cgacttccgc
    13801 cgcatggaaa ccgacaaaaa aggggaaaat gcccccgaca ccaagcgtat taaagaaacg
    13861 ctggaaaaat tcagtttgga aaatatgcgt tatgtcggca ttttgaagtc tggacagaaa
    13921 gtctccggct tcatcgaggc tgaaggttat gtctacactg tcggtgtcgg caactatttg
    13981 ggacaaaact acggtagaat cgaaagcatt accgacgaca gcatcgtcct gaacgagctg
    14041 atagaagaca gcacgggcaa ctgggtttcc cgtaaagcag aactgctgtt gaattcttcc
    14101 gacaaaaaca ccgaacaagc ggcagcacct gccgcagaac aaaattaaga agaggattac
    14161 tccattatga ataccaaact gacaaaaatc atttccggtc tctttgtcgc aaccgccgcc
    14221 tttcagacag catcggcagg aaacattaca gacatcaaag tttcctccct gcccaacaaa
    14281 cagaaaatcg tcaaagtcag ctttgacaaa gagattgtca acccgaccgg cttcgtaacc
    14341 tcctcaccgg cccgcatcgc cttggacttt gaacaaaccg gcatttccat ggatcaacag
    14401 gtactcgaat atgccgatcc cctgttgagc aaaatcagtg ccgcacaaaa cagcagccgt
    14461 gcgcgtctgg ttctgaatct gaacaaaccg ggccaataca ataccgaagt acgcgggaac
    14521 aaagtttgga tattcattaa cgaatcggac gataccgtgt ccgcccccgc acgccccgcc
    14581 gtaaaagccg cgcctgccgc accggcaaaa caacaggctg ccgcaccgtc taccaagtcc
    14641 gcagtatccg tatccaaacc ctttaccccg gcaaaacaac aggctgccgc accgtttacc
    14701 gagtccgtag tatccgtatc cgcaccgttc agcccggcaa aacaacaggc ggcggcatca
    14761 gcaaaacaac agacggcagc accagcaaaa caacagacgg cagcaccagc aaaacaaacc
    14821 aatatcgatt tccgcaaaga cggcaaaaat gccggcatta tcgaattggc tgcattgggc
    14881 tttgccgggc agcccgacat cagccaacag cacgaccaca tcatcgttac gctgaaaaac
    14941 cataccctgc cgaccacgct ccaacgcagt ttggatgtgg cagactttaa aacaccggtt
    15001 caaaaggtta cgctgaaacg cctcaataac gacacccagc tgattatcac aacagccggc
    15061 aactgggaac tcgtcaacaa atccgccgcg cccggatact ttaccttcca agtcctgccg
    15121 aaaaaacaaa acctcgagtc aggcggcgtg aacaatgcgc ccaaaacctt cacaggccgg
    15181 aaaatctccc ttgacttcca agatgtcgaa atccgcacca tcctgcagat tttggcaaaa
    15241 gaatccggga tgaacattgt tgccagcgac tccgtcaacg gcaaaatgac cctctccctc
    15301 aaagacgtac cttgggatca ggctttggat ttggttatgc aggcacgcaa cctcgatatg
    15361 cgccaacaag ggaacatcgt caacatcgcg ccccgcgacg agctgcttgc caaagacaaa
    15421 gccttcttac aggcggaaaa agacattgcc gatctgggcg cgctgtattc acaaaacttc
    15481 caattgaaat acaaaaatgt ggaagaattc cgcagcatcc tgcgtttgga caatgccgac
    15541 acaaccggaa accgcaatac gcttgtcagc ggcaggggca gcgtgctgat cgatcccgcc
    15601 accaataccc tgattgttac cgatacccgc agcgtcatcg aaaaattccg caaactgatt
    15661 gacgaattgg acgtacccgc gcaacaagtg atgattgagg cgcgtatcgt cgaagcggca
    15721 gacggcttct cgcgcgattt gggcgttaaa ttcggcgcga caggcaagaa aaagctgaaa
    15781 aatgatacaa gcgcattcgg ctggggggta aactccggct tcggcggcga cgataaatgg
    15841 ggggccgaaa ccaaaatcaa cctgccgatt accgctgccg caaacagcat ttcgctggtg
    15901 cgcgcgattt cctccggtgc cttgaatttg gaattgtccg catccgaatc gctttcaaaa
    15961 accaaaacgc ttgccaatcc gcgcgtgctg acccaaaacc gcaaagaggc caaaatcgaa
    16021 tccggttacg aaattccttt caccgtaacc tcaatcgcga acggcggcag cagcacgaac
    16081 acggaactca aaaaagccgt cttggggctg accgttacgc cgaacatcac gcccgacggc
    16141 caaatcatta tgaccgtcaa aatcaacaag gactcgcctg cgcaatgtgc ctccggtaat
    16201 cagacgatcc tgtgtatttc gaccaaaaac ctgaatacgc aggctatggt tgaaaacggc
    16261 ggcacattga ttgtcggcgg tatttatgaa gaagacaacg gcaatacgct gaccaaagtc
    16321 cccctgttgg gcgacatccc cgttatcggc aacctcttta aaacacgcgg gaaaaaaacc
    16381 gaccgccgcg aactgctgat tttcattacc ccgaggatta tgggtacggc cggcaacagc
    16441 ctgcgctatt gatgcgtcaa aataagggca tatgttttac ggcatatgcc ctttctttat
    16501 gctttttgcc gcgaccgaaa tgccgtcatt cccgcgcagg cagaaatcca acttgtccgg
    16561 ttccggttgt ttttcgtctc gtaacttttg agccgtcatt cccgcgcagg cagaaatcca
    16621 gtccgttcag tttcggtcat ttccgataaa ttcctgttgc ttttcatttc tggattccca
    16681 cttttgtggg aatgacggcg gagaggtttt tgttgtttcg gagaagtttc tgcaacccta
    16741 gaatctcgtt atttccacaa aaaacagaaa cccgaaacag caacttaaaa cctcgtcatt
    16801 cccgcaaaag cgggaatccg gtccgttcag tttcggtcgt ttccgataaa ttcctgctgc
    16861 ttttcatttc tagattccca ctttcgtggg aatgacggcg gagaggtttt tgttgtttcg
    16921 gagaagtttc tgcaacccta gaatctcgtt atttccacaa aaaacagcaa cccaaaacgg
    16981 caacttaaaa ccccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg ttcaggacct
    17041 tatcggataa aacggtttct ccaaccctgc gttctagatt cccactttcg tgggaatgac
    17101 gggatatggg tttccgtgcg gacgtgttcg gatttccgcc tgcgcgggaa tgacggcgac
    17161 agatgcccaa cggtctttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
    17221 gacagtacaa atagtacgga accgattcac ttggtgtttc agcaccttag agaatcgttc
    17281 tctttgagct aaggcgaggc aacgctgtac tggtttttgt taatccacta tagtattgat
    17341 aaacatatta tcttcaatat attcaattgg ataattgttt acctaagcaa agataattgc
    17401 cttttcctga caaataagtg aaatcaacgg attgtcaaaa cacagcctga aataaaaaac
    17461 ctccctgatt tcttttattt ttccttaaaa tcagaaaggt tcgggatggt cgggttattt
    17521 ttccaaacgt accgctgccc tgccgatttc gtataaaatt ccgccgtaac ccgacaagcc
    17581 cgaaccctgt cgccccgaaa ggcggggtgt caaacattaa ggaattgtga tgaaaaactt
    17641 taacggcaaa ctcatcctca tcggactgat gggcgcgggc aaaaccacgc tgggccggca
    17701 aatggcgcag cggctggatt accgttttta cgacagcgat cacgaaatcg ccgtagccgc
    17761 cggcgttccc atccccacca tatttgaaat ggaaggcgaa cagggattcc gttcgcgcga
    17821 aaccgccata ctcaaaaagc tggttatcct gccccatatc gtcctgtcca ccggcggcgg
    17881 cgcggtgtta aaagaagaaa accgcgccct tatccgcaaa agcggcacgg tcgtctatct
    17941 gcacgccccg cccgaaaccc tgctcgaacg cacgcgctgc gacaacagcc gtcctttgct
    18001 gcaagttgcc gatcctttgg cgaaattacg tgaactctac gccgcacgcg accccgttta
    18061 ccgccaaacc gccgacttta ccgtagaatc ggcaaactgc cgggaaaccg tgcaaaccct
    18121 gctcaaacgc ttatcccgat aaaccggcat atgcgccgcg cccagaaaac caagccgcgc
    18181 cccgcccggc gggccggcgg ttcaaacttt aaggaacaac aatgaaaaca ctgaccgtac
    18241 acaccccgtc ccacagctac cccatcttta tcggcaacgg gctgctgccg caggcaggca
    18301 gcctgctcaa accgcatttg ggcaaacgcg ccgccatcat cgccaacgaa accgtcgccc
    18361 cgctctacct cggcacgctt cagacggcat tggatgcggc aggcgtatcc catttcagca
    18421 tcatcctgcc cgacggcgag gcgcacaaaa actggcagac gctcaacctc atctttgacg
    18481 ggctgatgca aaaccgcgcc gaacgcaaaa ccacattaat cgcactgggc ggcggcgtga
    18541 tcggcgacat ggtcggcttt gccgctgcca cctaccagcg cggcgcaccg ttcgttcaaa
    18601 taccgaccac actgttgagt caagtcgact catcggtggg cggcaaaacc gccatcaacc
    18661 acccgctcgg caaaaatatg atcggcgcat tctaccagcc gcaggcggtg cttgccgact
    18721 tggacacgct gcacaccctg cccgtccgcg aattgtccgc cggtatggcg gaagtcatca
    18781 aatacggcgc gctcggcgac atcggttttt tcgaatggct cgaacaaaac atatccggac
    18841 tgatgacgct cgatcgggaa aaactcgccc aagccgtgta ccgctgctgc caaatgaagg
    18901 cagacatcgt cgcccaagac gaaaccgaac agggcatacg cgcatggctc aacctcggac
    18961 acaccttcgg acacgccatt gaaaccgaga tgggttacgg cacttggctg catggagaag
    19021 ccatcgccgc cggctgcgtg ttggcggcgc gtttgtccga acaactgggc aaaacctccg
    19081 ccgcagatac cgcgcggctc gccgccctgc tcgaagccgc tggactgccg tccgcaccgc
    19141 ccgtgtttgc ctttgaaaaa tggctcgaac atatgagcca cgataaaaaa gtcagcggcg
    19201 gcatcatgcg ctttatcggt ctgaaccggc tgggcgaagc caacatcacc gaaattaccg
    19261 acacggacat cctccgccgc accctgcaac cgtatctctg atttcctctg ccgatgtgct
    19321 gccgcgcggg tttgacgcac gatgatgatt ttccatcatc tttctccgca aaagcgggaa
    19381 tccagtccgt tcggtttcgg tcgtttccga taaattcccg ttgcttttca tttctagatt
    19441 cccactttcg tgggaatgac ggcggagagg ttttggtttt ttccgataaa ttcttgaggc
    19501 attgaaattc tatagtgaat taacaaaaat caggacaagg cggcgagccg cagacagtac
    19561 aaatagtacg aaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag
    19621 ctaaggcgag gcaacgtcgt ac
//
LOCUS       DAWFKN010000032        19599 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFKN010000032 DAWFKN010000000
VERSION     DAWFKN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.032"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGR46_001118"
     CDS             complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGR46_001118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0856069.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1262..2455)
                     /locus_tag="WGR46_001119"
     CDS             complement(1262..2455)
                     /locus_tag="WGR46_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0856070.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(2491..4350)
                     /locus_tag="WGR46_001120"
     CDS             complement(2491..4350)
                     /locus_tag="WGR46_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0856071.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            4624..5403
                     /locus_tag="WGR46_001121"
     CDS             4624..5403
                     /locus_tag="WGR46_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0856072.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(5614..7026)
                     /locus_tag="WGR46_001122"
     CDS             complement(5614..7026)
                     /locus_tag="WGR46_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0856073.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            7117..7193
                     /locus_tag="WGR46_001123"
     tRNA            7117..7193
                     /locus_tag="WGR46_001123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7151..7153,aa:Arg,seq:acg)
     gene            7211..7285
                     /locus_tag="WGR46_001124"
     tRNA            7211..7285
                     /locus_tag="WGR46_001124"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7244..7246,aa:Glu,seq:ttc)
     gene            7841..9016
                     /locus_tag="WGR46_001125"
     CDS             7841..9016
                     /locus_tag="WGR46_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0856074.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9226..9774)
                     /locus_tag="WGR46_001126"
     CDS             complement(9226..9774)
                     /locus_tag="WGR46_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0856075.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(9777..11273)
                     /gene="ccoG"
                     /locus_tag="WGR46_001127"
     CDS             complement(9777..11273)
                     /gene="ccoG"
                     /locus_tag="WGR46_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0856076.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            11801..13780
                     /gene="tkt"
                     /locus_tag="WGR46_001128"
     CDS             11801..13780
                     /gene="tkt"
                     /locus_tag="WGR46_001128"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0856077.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVTWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            13921..15309
                     /gene="fumC"
                     /locus_tag="WGR46_001129"
     CDS             13921..15309
                     /gene="fumC"
                     /locus_tag="WGR46_001129"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0856078.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(15374..16297)
                     /locus_tag="WGR46_001130"
     CDS             complement(15374..16297)
                     /locus_tag="WGR46_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0856079.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAVGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            16469..16609
                     /locus_tag="WGR46_001131"
     CDS             16469..16609
                     /locus_tag="WGR46_001131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856080.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(16669..17193)
                     /locus_tag="WGR46_001132"
     CDS             complement(16669..17193)
                     /locus_tag="WGR46_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0856081.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(17197..18579)
                     /locus_tag="WGR46_001133"
     CDS             complement(17197..18579)
                     /locus_tag="WGR46_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0856082.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(18690..19313)
                     /locus_tag="WGR46_001134"
     CDS             complement(18690..19313)
                     /locus_tag="WGR46_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0856083.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         4823 a         5651 c         4694 g         4431 t
ORIGIN      
        1 ccatgccgtc tgaaaaccgt tcagacggca tattttttgg acggcgcgcg taaagcggtt
       61 tacgcccaca aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata
      121 aggcggaacg cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc
      181 agcagagctg cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg
      241 aaatcgtagg tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt
      301 tgccgccaca agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg
      361 tcttcgggca gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg
      421 cgttcgtccg taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt
      481 aagagttcgc cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc
      541 atttttttca gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa
      601 aatgccatga ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac
      661 gcgattttcg ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc
      721 gcaaaaatgg cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa
      781 ttacgggtaa cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa
      841 cgcctgaata cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc
      901 acatacaccg cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc
      961 ccgaactgcc gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg
     1021 acgaccaccg gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaat
     1081 gcgtccatgt cgatgtggat aattttgcgt gaagacatcg gctcttctga ggataaaagg
     1141 gatattctac tgccggcatc gggcaaattc caaatatacg ccccgataga cctgcctcca
     1201 taaaaatgcc gtctgaaaca taccctgttt cagacggcat ccgcaaaact acggttttca
     1261 attaaaactg ccaatccagt ttcatgctga cagtgcgcgg ctctccgtag aagttgtttg
     1321 cgccgcgcgt acggttgtag ttgttctcaa aataagtgcg tccgtttaag ttcgtaccga
     1381 tgaggctcaa tttggcgtgt ttgcccaatt cgtaacggac gaaaccgtct atcagcccgt
     1441 agccgccctg acggatgttg tacaggctgc ttgtgccgct ttgtgcggac acgccgccgc
     1501 cgacggtcag ccccgtattc ggtatatgga agctcgttcc gaaacggaat atgtgcacgg
     1561 gtgtgaaatt gctgaagttg tacgggtctg cgcctgtgtt tttggcgagg cgttcggcgt
     1621 tgacttcggc ggcgtttttg tagcggctct tgttgtaggt gtaacccgca aagactttcc
     1681 aatcttcgtt caactcaccc gacaactcga attccgcacc cctgctgacc actttgccta
     1741 tcggtttggc aacggtttgg aacgacccct gcttgccgcc tgctccggga acatagccaa
     1801 aatcgacgac cgtgcggttt ttctgttcga ggtaaaacaa tgcgaacgaa gcattcagcc
     1861 gtccttgcaa gaacgcgcct ttccagccta cctcatagtt tgtgccgacc aaaggcggta
     1921 aaacggtttt ggcactgaca tcgacattat cctgctgttt gaagattttg gtataacttc
     1981 cgtaaatact ctgttgcggt gtcaaatcat aggtaatgcc tgcatagggc gtcaatttat
     2041 gaccttgcat cttggccgta taatggtcct gatccgcctt aatgctcgat gccgtctgaa
     2101 aatcgcttgc cggctgccca tagcggacag gcatatcttt ggtttgcgag gtttcatagc
     2161 gcgtgtagtg cagcccgccc aaaagatgca gtcggccggt tacgttgaaa cgcgtgccgg
     2221 cagtcagcga atgggttttg ttggtgttga ggtatttggc gtagttatac agcgcaggaa
     2281 catggtcgtc ggccactttg acggttttcc aaaccggcac cgtaccggaa aaaccggtaa
     2341 aggcaggcgt gccgtcggga ttggtctcct gaatcttatt gcctttttca tccagttcat
     2401 atacatcgac atatactggc gtccggctgc cgctgtattc gtcatagtaa tacacctgct
     2461 tgccttcggc atcgagcttg ggctcggttt ttattttctt ggcattcctg cattcttcgg
     2521 cataaacggt acggttgcct ttttcatcgt acgcctgcca atcgggttct ttatgccccc
     2581 tgaccaaagg agaagacaaa tcaccgtccg gctcccctga caacttcccg catacacgcc
     2641 gtgcgttgcc cctgtattcg gacgtactct gtggcggcgt tcgtagattt ccagatattc
     2701 ggaacgtatc ttttcatcgc cgtaggcata gccgacaaag aaatcatgct cccgcccgaa
     2761 cagcccatat gtgccggtca ggtcaagttt aattccccat tggcggtcgt ctttggtatg
     2821 ccgcaacggc atataactgt atcgtcggtt ggcggtagcc ttccgattaa aggaagagtc
     2881 atacaggctg tttggaaaac gttgtgccgc attattaaag atgccttcct tcgcaagggc
     2941 tttatcgaca aattccgact tgtcggcatc aacacccgga tccccccaag aaccttgaca
     3001 gataaagtcc agcgcaaaag gggcactcat acactcgtca aaaccggctt tgcgttctgc
     3061 ggcacggcgg ctgcggtact gctcgaaagc agtattatcg aaacggtttt taacaaaatc
     3121 gtctttgcgc tcacggtatt ccttggcggt ttcgtcacga tatgctttca gtttctccaa
     3181 tgccttatct ttcggctcga acgggatgac ttcgtttttt tcggtcaaaa agcctaccgc
     3241 atcctcatct gacaaacccg ccgcatgttc gtttttcaga aaaaactgcc ccaccttcgc
     3301 atcggattca ttcttggtat aagacacttc ggcattgagc tgccaaccgt tgtcaaacac
     3361 atgtttgaaa cctgagaaaa ggttgtattt gtcagcactc aaccgcgacc aatcctcccc
     3421 caaataagtg ttgcgcggca gttgcaaagg ccggttgcag gcaggcgttg aactgaacgg
     3481 ggcagttttc tgattttcac aggacaaaat aatgcccgaa aaatcaggaa cctccctact
     3541 cttctgatac atgccgccca aagtaagcac actgctgtcg cccgcatcgg cttcggcaat
     3601 gccgtaaacc atatgtttcc tgccccaaac gcggtcttta aacgattttt tatactcttc
     3661 cgcacccacc aaccttccgc gtaaggtatt cgccttattc aggctgcctg aaacatccaa
     3721 cactgcacgc cggctgccgc gatggtcggc ggtcagctct ccggtatgtt tgaaagaagc
     3781 ggtaggtcgc ttacggatca aattgacggt tccacccggc tctgaattgg attgggtcaa
     3841 ccccgttgca ccccgtacaa cttcaatatg gtcataaacc gccaaatcgg tactcggaga
     3901 cacgtcgatt ttcgccgtat atcccgaacg gcctgcaaca ttgacggtaa taccgtcttc
     3961 accaatctga tcaatataga aaccgcgtga caaaaaccgc gtctgcaagc ctgaatcgcg
     4021 cacaacgttg acacccgtcg tgtttttcat tgcctcttca agcgtatgca ccgccttatc
     4081 gtcaaggcgg ctgcgcgtga tgacgctgac cgactgcggc gtatccttgc ccgcaatcct
     4141 catacctgtg gcggtggaca tccgatctat cgtataagaa cgggtcttct cggtcttgcc
     4201 cgacagagca tgagagccgc gtacattgac cgtatccaga ctgacggtat tgccgtctga
     4261 aacaggcaca acaccgtctg caaaagaacc accgtaagcc gataacagca taacggtcag
     4321 aattttaagt gaaaaatgat tttgattcat agagacctct gtaatatgca agtgtgcaaa
     4381 tcgtccaaag gccctcacaa ctgttttgat tttttatatt aattgaaaaa aagtaattct
     4441 caattaaatt tatagatggg attgtattcc cattttgaca aaaaacaaac tgctccttac
     4501 cgtttatttc aaaaaacgat aatattgtat tgaaaaatat ccgaatttaa atacagatcg
     4561 tcaatgcgga aaaaaacacc caaattggct ataatcccga caaacacact caaggacaac
     4621 aacatggcag cctcgcccga agcaaaattc actgaagaaa agattttgtg ggtcaaacac
     4681 cacacgccga aactcatcac tttcgccatc agccgtcccg aatcctaccg ttttaaagcc
     4741 ggacagttct cccgactcgg tttctacgaa ggggaaggct ttatttggcg tgcctactcc
     4801 atcgtttccg cagaatatgc agacacgctc gaatattttg ccgtactcat ccaagacggc
     4861 cccatgtcgg cccgtttcgc caaaatgcaa cagggcgaca ccatcctgct cgataaaaat
     4921 gccaccggct tcctcctgcc cgaacgcttc cccgacggca aggatttggt gatgctctgc
     4981 accggctccg gcatcgcccc cttcctttcc attctcgaac aacctgaaat ccgacagcgt
     5041 ttcgataccg tcaacctgat acattccgta tcttttcccg aagaattgat tttcaacgac
     5101 cggctcgccg cattgtccga acatcccctg gtaggcgaat acagacactc gttccatttc
     5161 gtccctgtta ccacccgtgc cgccaacccc tcgggcttaa gcggaaaacg cattccggaa
     5221 ctcttaaaaa acagcagcat cgaacaggcg ctgcatacca agttcacccc ggaatccaca
     5281 cggtttatga tttgcggcaa cccggaaatg gtcaaagata ctttccaaac gctgcttgac
     5341 atgggttacg ccatgcaccg caaccgcatt cccggtcaaa tcatgatgga aaacggcttc
     5401 taaaaaccac cctgcttgtc cgatgccttc ggatggacgg tcaaaccgac acggcacgaa
     5461 aaccgcgtcg gcaaaaatgc cgtctgaaaa aattcagacg gcatcttcgg acacattacc
     5521 tgcaaacggc aacacattcc aacccaaacc gattaggcaa tcaatacgaa cggctgttta
     5581 catacttacc ggctttcatc aaccggtatc gatttaaccg atttccttaa tatttttcct
     5641 gtccgtttta aacttcgcct taaacgcatc cggtaaatct ttatcgaaat accaaagccc
     5701 gtcacccatt tccaatgcgc cgcccattcc atgcagaaca actttttccc ccgttaaagg
     5761 atcagtttcg gtaatttcta cctcatgcat ttttccccaa tccaaaatag gcaacttgtg
     5821 tgcaaacgcc aaaacctgtt cgtcagaacg gccgcacgcc ttatcgcatc ggcctgaaac
     5881 atatacggga caaggctcat cctctgcata accgtccgga aggataccgg ctgaggcagg
     5941 acacaatccg tatgtttccg cccacgacga caaagcccgt ctcgccgcct ttttattggc
     6001 aaataatccg gtaggcggat tatccgtcac accgtttttc aaagccgctg ttttcgcatt
     6061 caacatgccg tctgaacctt tttcaaacct gacggtcgta aatgttttaa gcagattttt
     6121 ggcagataca taacaacccg aatgataacg tccgaccaat tccgctttaa tcttatatgc
     6181 atgcaggctg cccaatgcag gaaaaaaacg gacttcctcc gtattgcacc aatcaaacgg
     6241 ggcttttccg gaatccaata aagccgaaac ctcgctatat acccgttcaa acgtaccgat
     6301 gtaatttact ttccctccgc cgtcgaaaca agccagcacc cccataccgt caggcaaacc
     6361 gtacaactgt tccctcaacc gttcgggcag cgcggcaggc agcggtttcg gattcatcaa
     6421 acggaaacac tgcctgatcc atgcctcaac cccatgttcc gacaaactgt attccaaata
     6481 atcacacaat gccgacacat ccgccatcgc acgatgcctg tcttctacaa caatccccaa
     6541 cctttcgatg atactgtcca ggctgtgctt gtaaaattgc ggatacagac accgggacag
     6601 ttgcacactg cacaaagcag gcgatgaaaa cccgatgccc gcacgatgaa actcatgctt
     6661 taaaaacgta tagtcgaaac ggctgttatg tgcaaccagc acacaaccct tcaataccga
     6721 aaacaactcg ccggcaatct ctgcaaaaac aggcgcatcg gcaaccatgc cgtctgaaat
     6781 ccccgtcagc cccgccacaa actgcggaat cggtttttga ggattaacca accactcatg
     6841 cctcaccacc cttccctgct caaacttgac caaagccact tcggttaccc tgtcttcata
     6901 cagattgccg cccgtcgatt ccaaatcgac cacggcaaca ggcattccaa accgtaaaaa
     6961 taccttttcc agcaagggcc agcgggaagc aacaatcatt ttattctctt taaattcaaa
     7021 caacaaaccg atattttaca cttttaaggc atttcatcca acaaaacaat tgacagaatc
     7081 cgatgattac cctaaaattc aaatctttct tgcagtgcac ccgtagctca gttggataga
     7141 gtatctggct acgaaccaga gggtcgggcg ttcgaatcgc tccgggtgcg ccagtaagaa
     7201 aatacaatat gcgcccatcg tctagcggtt aggacatcgc cctttcacgg cggtaaccgg
     7261 ggttcgattc cccgtgggcg tgccaaattc ccaatccctg agattatccc tcggggattt
     7321 cttattacct cgacaactcg ttaccatatc tttacccccc ttcatcagac tctcagacgt
     7381 aatcgaatca tattcaaacc tttgccatgc aaaccgatat cacgtaaccg gatgcggtgt
     7441 ccgtccaaca ttttacccga ttgaaacgcc tgatatattg caccccatca acgtggcatt
     7501 acttttctta acaatcccct ttgacagcaa ctgactaggg cttttttatg ccatcatcaa
     7561 atttatagtg gattaacttt aaaccagtac ggcgttgcct cgcctttccg tactatttgt
     7621 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa ttcactataa tattttctct
     7681 cccgattgaa acaggcgtaa cagaatatgc ccgaagctcc ggctgctttc ttgtttaccg
     7741 ccgcgatatt tagagtataa taccaaattt gagcaatagt tctaaaacag ttagaaccat
     7801 ttttcatgag cctgactgat tcgtacactc ggagaaactg atgcagaata tttttgaccc
     7861 tttggttatt cgtggaaaat cccttacccc catcgtgcaa ggcggtatgg gggtcggtgt
     7921 ttccgcatcg ggtttatcca gcgcggtggc acgtgaaaac ggtatcggaa cgattgccag
     7981 tgtggatttg cgccaccttc acgaagacct actcgccgaa tcacaaatca atccgagtga
     8041 agagaaatat acatctttga actgtaccgc attagacagg gaaatccaaa aagccaaaag
     8101 cgcttcagag ggaaaaggac tgattgcggt caacgtgatg aaggcggtca aagaccacgc
     8161 cgcgtatgtc cgccaggctt gcgaatcagg ggcggatgcg gttgtaatgg gtgccggtct
     8221 gcctttagac ctgccggaaa tgaccgaggg ctatcataaa gatgtcgcgc tgctgccgat
     8281 tctgtccgaa tcgcgcggta ttaatatcgt cttgaaacgt tggatgaaaa aaggcatatt
     8341 gcccgatgcg attgtagtcg aacatcctgc ccacgcggcc ggacatttgg gtgcatcaac
     8401 cgttgaaggc gtaaacgatg ccaagttcga cttcaaacgc gtgattgagg aaacgtttga
     8461 agttttcaaa agtttagggc tggaaagcga aaaaatcccg cttattcttg cgggaggcat
     8521 ggcaaatttt gaaaaagtca aaaccgccct aaagaactgg ggagcatccg ccgttcaaat
     8581 cggtacggct tttgccgtta ccgaagaagg agatgcacac cttaacttca aaaaaacgct
     8641 cgccggtgcg gaaactgaaa aagtagtcga atttatgtct gttgccggtt tgccggcgcg
     8701 cggtgtccgc accaaattcc tagacagcta catcaagcgt gaaagcaaac ttcagacaaa
     8761 cgccaaagcc gacccgcgcc gctgtaccca aggtttaaac tgcctaacca gttgcggtct
     8821 gcgcgacggg ctttccaaag ccggacaatt ctgcattgat atccagcttg ccgccgcatt
     8881 ccgtggagaa gtagataaag gcctgttctt cagaggtaaa gacccgctgc ccttcggcaa
     8941 tgccatccgc accgtccgcg agacgataca atatctgctg acggggagcg aacctgttgc
     9001 aacgctcgga cgctgacatc aggtaggatt tgatttgcaa acaaaatgcc gtctgaagcc
     9061 cttcagacgg catttttcag gctgcatttc ggaatggtgt taaaaaataa acgggataag
     9121 atacatttat tttcgtccga taaatcagac catcacgccg aataatcaaa caatgcctgc
     9181 ccgacattgc atctggcaaa ccaatgcaat gaaaacacgg cttttttatt tgcttccggc
     9241 attattgaaa agtttatcca tcggggtcag atccaccgca ttgccttggc tggtaatcca
     9301 tttgttctcg acgcgccaca cgcctgccgc gtcctccacg cgcacatacc agtggttggt
     9361 cggcgaaagg gttttgaaca ccgcctcata ttccgccctg ccgttctgcg cgctgccgac
     9421 gggcttgagg gcgacggttt gatcgtccgc cttgcgggtc gggtgcatca gcagcaggtt
     9481 caaaggctgt ttgccgtcaa actcgccgcc gacaaacact tttgccgcat tcatatcggg
     9541 ggaaatgaga acctgcaccc cgatatgccg tctgacagcc tcttcatccc gatgaagctg
     9601 gatgtcgata tgtttgccgt ctttataata atcgtccgta accaaatctg tcgcgtgctg
     9661 ctgtgcgaca aaaaacatag cgacgctggc gatgacgaca aaaatcggcc ccgccatcaa
     9721 gatccacggc cagacgtgtt tgtaccaagg tttgattgga gtgttttgag acacggttat
     9781 tctccgataa aggttgcatc ttcttccaag acgaccggct tgccgtcggg cgcgccgctt
     9841 tcgcggtatt ggaaggtaaa ttcgataggg tggctgcctt tgtccgcgta ttccggaatg
     9901 gtggacactt ggacgggaag ggttaccgtt tcgcgcgggg caaccttgat accgccttcg
     9961 ggcagcccgg tcagggcgat ttcgtcaaag cctttgacac ttgcggtaat gagctgttct
    10021 ttttcacttt tgttgatgat acgcaggctg tatgcgtttt ccagccagcc tttggcgttt
    10081 tcgcgcacca gtacgccacg gtctttcaaa atatcgacct cgaccatttt gcgcgtggac
    10141 aaaccggcca ggaaggcaat gataactaac gccaacaccg cgccgtaacc tgccacgcgc
    10201 ggtctgagca gccgtttttt aatgtctttt tcagaatatt cgtgttccag cgcgctttcg
    10261 gtcgtataac ggattaatcc gcgcggatag cccattttgt ccataatctc atcgcacgcg
    10321 tcgatacagg cggcgcagcc gatacattgg tattgcagac cgttgcggat gtcgatgccg
    10381 acggggcaga cttggacgca catcgcacag ttgatgcagt cgcccaaacc cgcctcttcc
    10441 ttattgaccg ttttcttgcg cgcgccgcgc ggttcgccgc gttccgcgtc ataagaaaca
    10501 atcagcgtgt ccttgtcgaa catcgcgctt tggaaacgtg catacggaca catatgcagg
    10561 catacttttt cacgcataat gtgggcgaag aagaaggtca taaagccgta aaacgctgcg
    10621 gcaaacatcg cgccgccacc tgctgctcca gtgaataaat cgggaacgaa ctggcggata
    10681 gggacaaacc agcctgcaaa cgtgatgccc gtccacgcgc agacaaggaa aatcagcagg
    10741 tatttggtgg ctttgatgcg gattttagtg aaattccacg gcgatttttc cagtttcagc
    10801 cgtttgtttc tatcgccttc gaccaggttg tcaatccaca gcataatttc ggtgtaaacc
    10861 gtttgcgggc aggaatagcc gcaccacagt cgccctgcaa tcgtcgtcca ccaaaacagc
    10921 ccgaaggcgc aaatcatcag cagcaaggca aggtaaatca aatcgcccac ccccaacgac
    10981 aatccgaaaa tgaagaaatg ccgttcgggg atattgaaaa cgacggcctg cctgccgctc
    11041 cagttgaacc acggaatgac gtaaaacaca aactgcgtcg ccaatacggc ggcgatacgc
    11101 agtttggcga accgtccttc cgcctttttg ggatggatgc gttcgccttc gggatggatt
    11161 tgaatcacgc tggctcgcgg atcgaatgtt ttttttgctt tcggcgcggc ttttgtttgt
    11221 tcggacgtgc cgattccgga tgccggactg ccggcttggt tttccgtggt cattctgcat
    11281 tccttagatt tttgattgat ggtttgcccg ttaccgccgc cgtttgcttt tcagacgtca
    11341 tttttcttgt tttttaaggc gttgtgtttc aagttttgag aaaatccgtt tttcccaaaa
    11401 tatatttccg ctattgtaca actttatgcg ccgtccggat gtatggggcg gatacatttc
    11461 ccatccgcat caaaacgcct ggattttacc ttaccgcccg aacaaaatcc gaatacggtt
    11521 aaaaaaaaag actaaaaaaa ccgacacccc catatcggca gaaccgacgg cgcaagctca
    11581 taaacaaacg ctatcgacaa tccggcacac aatctataac tttttatttc aaaaggaata
    11641 atggcaggct tcgcccgcaa atcgaaaatc cttccccgcc tgtcccctgc cgccgccttc
    11701 ccacgcgtcc gcccttttct tgaaagcata agcgaatcgg gcgataatca acgctttccg
    11761 attatccact tatctgaaac accagcaagg aaaatacaaa atgtctcaac tggcaaacgc
    11821 aatccgcttc ctctcggccg atgccgttca aaaagccaat tccggccacc ccggcgcgcc
    11881 tatgggtatg gcggaaatgg cggaaacatt gtggacgaaa ttcctcaatc acaaccccgc
    11941 caaccccaaa ttctacaacc gcgaccgctt cgtcctctcc aacggccacg cgtctatgct
    12001 gttgtacagc ctgctgcacc tgaccggcta caatctgagc attgaagact tgaaaaactt
    12061 ccgccagctg cacagcaaaa cccccggcca ccccgaatac ggctacaccg acggcgtgga
    12121 aaccacgacc ggcccgttgg ggcaagggat tgccaacgcg gtgggtatgg cattggcaga
    12181 aaaaatcctt gccgccgaat ttaataaaga cggtttgaac atcgtcgatc attacaccta
    12241 cgtctttatg ggcgacggct gtctgatgga aggcgtatcg cacgaagcct gttcgctcgc
    12301 cggcaccttg ggcttgggca aactgattgt tttgtatgat gacaacaata tttccattga
    12361 tggtaaagtg gacggctggt ttaccgaaaa catcccgcaa cgctttgaaa gctacggctg
    12421 gcacgtcgtt cccaatgtaa acggtcatga caccgccgcc attcaagccg ccatcgaagc
    12481 cgcacgtgcc gaaaccggca aaccgtccat catctgctgc aaaaccttaa tcggcaaagg
    12541 cagtgccaac aaagaaggca gccacaaaac ccacggcgca cctttgggcg cggacgaaat
    12601 cgaagccacg cgcaaacatt tgggctggac ttaccccgcc tttgaaatcc cgcaagaaat
    12661 ttacgatgcg tggaatgcca aagaacaagg cgcgaaactg gaagccgact ggaacgaact
    12721 gttcgcgcaa tatcaagcca aatatcctgc cgaagccgca gaatttgtgc gccgtatgga
    12781 taaaaagctg ccggacaatt tcgatgaata cgttcaagcc gcattgaaag aagtgtgcgc
    12841 caaagccgaa accatcgcca cccgcaaagc cagccaaaac agcatcgaaa tcttggcaaa
    12901 agagctgccc gaactggtgg gcggctctgc cgacctgacc ccgtccaacc tgaccgactg
    12961 gtcaaacagc gtttccgtta cccgcgacaa aggcggcaac tacatccact acggcgtgcg
    13021 cgagttcggt atgggcgcga ttatgaacgg tttggtattg cacggcggcg taaaaccctt
    13081 cggcgcgact ttcctgatgt tcagcgaata cgagcgcaac gccctgcgta tggctgcgtt
    13141 gatgaaaatc aaccctgtat ttgtgttcac ccacgattcc atcggtttgg gcgaagacgg
    13201 cccgacccac cagccgattg agcaaaccgc caccctgcgc ctgattccga atatggacgt
    13261 atggcggccg tgcgacaccg ccgaatcctt ggtcacttgg gcagaagccg tcaaagccgc
    13321 cgatcacccg tcctgcctga ttttcagccg tcaaaacctg aaattccaag cgcgcagcga
    13381 gcaacaactg aacgacatca aacgcggcgg ctacgtcatc agcgaagcac aaggcaacgc
    13441 ccaagccgtc atcattgcca ccggttccga agtcgagctg gctttggaag cgcaaaaagc
    13501 cctcgccgcg caaaacatcg ccgtgcacgt cgtttccatg ccgtccacca acgtattcga
    13561 ccgccaagac accgcctatc aagccgccgt cctgcccgaa agcctgccgc gcatcgccgt
    13621 agaagccgga cacgccgacg gctggtacaa atacgtcgga ctgaacggcg cagtcgtcgg
    13681 catcaaccgc ttcggcgaat ccgcccctgc cgatttactc ttcaaagcat tcggctttac
    13741 cgtggacaat gtggtcgata cagtgaaatc agtgctgtaa ccccactcct aaacaaatgc
    13801 cgtctgaacg cttttcagac ggcatttttt tgccctcccg ccttgatgct tcctcattcc
    13861 accaatctcc attataatat ttgcgaatca ctcttattca catttcaaaa ggagaaacgt
    13921 atgaacactc gcaccgaaca cgacaccatg ggcaatgtcg aagtcccgtc cgaagcctat
    13981 tggggcgcgc agacccagcg cagccgcaac aatttcaaaa tcggcggcga aaccctgccg
    14041 cagccgctga tttatgcttt ggcgttggtg aaaaaagccg ctgccgcgac caatgtttcc
    14101 ctcggcagga ttaagccgga acaggcggat ttgattacgc aggcggcgga tgatgtgttg
    14161 aacggcaagc tcgacggaca gtttccttta gtggtttggc agaccggctc cggcacgcag
    14221 tccaatatga acatgaacga agttctggcg aaccgcgcca acgaaatcgc cggtacgggt
    14281 ttggcggcgt atcagcccgt ccatcccaac gaccatgtga accacgcgca atcgaccaac
    14341 gacgcattcc cgaccgctat ccacgttgcc gccgcgattg aaatcaaccg ccacctcatc
    14401 cccgccgtaa aagccctgcg cgacaccttg gacaaaaaag cccaagcctt cgcccctatc
    14461 gtcaaaatcg gccgcaccca cttgcaggac gcgacgccgc tgactttagg ccaagaattt
    14521 tccggctacg tttcccagct cgaccacggc ttaggccgtc tgaacgacgc gcttaaagac
    14581 ttgtatgaac ttgctttggg cggtacggcg gtcggcacgg gtttgaacag ccatcccgaa
    14641 tacgccgaaa aagccgccgc caaactcgcc gaattgtccg gcttgccgtt tgtcagcgcg
    14701 ccgaacaaat ttgaagcctt gggcggacgc gatgccgccg ttgccgcttc gggcgcattg
    14761 aaaacgctgg cggcaagcct gaataaaatc gccaacgaca tccgctggct ggcaagcggc
    14821 ccgcgctgcg gtttgggcga aatcaaaatc cccgaaaacg agccgggttc gtccatcatg
    14881 ccgggcaaag tcaacccgac ccaatgcgaa gcgatgacca tggtgtgctg ccaagtgttc
    14941 ggcaacgacg ttaccatcgg tatggcgggc gcgtcgggca atttcgagct gaacgtctat
    15001 atgcccgtca tcgcctacaa cctcttgcaa tccatccgcc tgttgggcga cgcgtgcaac
    15061 agcttcaacg aacactgcgc cgtcggcatt gaacccgtac cggaaaaaat cgactatttc
    15121 ctgcaccatt ccctgatgct cgttaccgcg ttaaaccgca aaatcggtta cgaaaacgcc
    15181 gccaaagtcg ccaaaaccgc ctacaaaaac aacaaatcgt tgcgcgaaac cgccgttgag
    15241 ttgggcttgc tgacgggcga agaatttgac gaactggtcg ttcctgccga tatggttcat
    15301 ccgcgctaat ctttccctca aataaaatgc cgtctgaaac ctcgttcgga cggcattttc
    15361 cgttgcctgc gaactagcgg cgtttgaaca gcctgtcccc caccgccgcc gtaaccgcac
    15421 ccccgaccac gaccagtgcg ccggcataac ccaaaccgtt catatccggc gcggcaaaag
    15481 tatcaggcat cacataatgc ccgagcaaag aaaatattac ggtaaacacg gggagcaagg
    15541 ttgttaccgc gctgactttg gaagcctccc aatgtttcaa cgcctcgccg aacgagccgt
    15601 aaccgattaa cgtattcaag cagcaatacg caaaacaaac ccacgccaac gtaccgtcca
    15661 aacttccgat gtgtgccagt tcggcaaacg gcaggaacac ggcggcactt gccgcataaa
    15721 tcaacagcag aatctgttgc ggcccgaatt gcgccgacag cagcttttgc gccacggcat
    15781 aacacaccca tgccatactg cctaccgcac acagcaacac gcccttcgca tacgcgccca
    15841 aacccgacaa ctcgccgaat ttatcgttaa aaaacataag caaaccggca agcagcaaaa
    15901 ccaagccgat tttctgagcg gcagtcatcc ggtctttaaa caccaacaca ccgacaacaa
    15961 tcatcgtaaa cggcgaaatc tgccacaaaa cctgcgtcgt ggtcggcgaa atataatgca
    16021 gcccttgggc aatcagcaca aagtttgccg aaatgcccgc cacgccgagc agcagcagcc
    16081 tgaatgagca ccaagaaaaa tcccgccact tcggcagccg cccgcccaat gccagcaaaa
    16141 caaacaatac cgccgccgcc acggtaaaac gcacccacac cagcgtcggc gcatcgacaa
    16201 acttcaatac ctgccgcacg gcaatcggca gcgttcccca cgtcatcgcc gccaaaagtg
    16261 ccaacgcgaa gcctaggagc ggcctttggt tttccatcct gattttccta tttttaaaca
    16321 accgtattgc cggacgatgc cggtttgccg catcgggcaa tgatggttca agcgtttgac
    16381 gtttgattcc aaccctttga tttcaaacaa accggctgaa gcccgactat tgcttcgcgc
    16441 tatttggaaa caccgcctga attttaaaat gccgtctgaa aacagcgggg acgtgcggca
    16501 ggcatcactg cctcaaaacg cccgaacggc gcaatcgcaa cgttccgccc aacgcaaggg
    16561 gcggcaacaa gccggctcgg atgcaaaaaa gagaaaccct gccccgtaag gtttaaggtt
    16621 tctccgtcct ttatgatttc cctctgcgag gatgtccggc cgtaaaattc agaacgggat
    16681 gtcgtcgtca atatcctcga ccggggcggc ggcaggcacg ggttggcggc gcggcgcggc
    16741 tggtgcttct tggggatggg acggcgcgtc ggaggcgggc tgccggcttt gctgtgcggg
    16801 gcgttggtaa gcctcctgac tctgaccgta accttcctcg taaggcgcac cgccgctgtt
    16861 ttcattgcgc ccgcccaaca ttttcatttc gttggcgaca atatcgtaag cggtgcgttc
    16921 gatgccgtct ttgccttggt atttgcggct ttggattctg ccttccagat aaaccagccc
    16981 gccttttttg aggtattgcc cggcaatttc cgccagtttg cggtacatcg tgatgttgtg
    17041 ccactcagta cgctctacac gttggccgtt gcggtcgttc caagtttcgc tggtggcgac
    17101 gctgaaatta cacaccgcct cgccgttggg catatagcgc acttcgggat cgcgtccgag
    17161 gcggccgata aggataactt tgttcaatga cattttttaa actcctgtga tgattttttc
    17221 agcggcagcc tgatcgaaac ccttctgcaa cactttgaga tagacggtct gcccgtcgaa
    17281 actgaaaccg atgtcttcca caccctcaag ctccgacaag gcgcggtata acacttcctg
    17341 attgacctgc cacacgccgc cgacagggta actgaggttt ttgacgggct tgggcgcagg
    17401 cgataaaacg gcaatcacca gccacagcag catcaatatg ctgcaaaagg caaacacgcc
    17461 ggaaaagccg tatttttgaa acagcaaacc gcctgccacg ccgccggcaa acagtccgag
    17521 cgactgcatc gtgttgtaca cgcccatcgc cgtacccttc agatcggacg gcgcgatttt
    17581 ggaaaccata gacggcaggc tcgcttccaa cacattaaaa ccgataaagt aaacaaccaa
    17641 ataagcggta atcaagccta ccgagcgcat accggacagc aaaccgagct gtgccgccgc
    17701 gatgcaggcg atgcccaaga taaaaacctg tttgagcttg ttgcgcgtct cgccgacgat
    17761 aatcagcgga accatcacca ccaagcccgt aatggtcgag ggcagataga ctttccaatg
    17821 ctggattttt tccaaaccga gctgcgtcat cgcaaaaggc agcgcggtaa acaatgccat
    17881 ttgcgcggcg tgcagggcga aaatgccgaa atcgagcgtc agcagcctac ggtttttcaa
    17941 aacttcgcct atgcgcgaag gctgcgcctg cgtatcttcg tgcagcttgg aaacctccgg
    18001 gtcgggagtc atccacgcca ccacgccgat actggcaacg gtcaaaatac cggtcagcag
    18061 aaacaagccg cgtacgccca ccgcgtcggc aatcacaggg gcaacgacga ggctgaccga
    18121 aaacgtcaga ccgatactca aaccgatcat cgccatcgcg cgggtacgta cgccgtcgcg
    18181 cgtcaaatcc gccagcagcg cggtaaccgc cgcactgacc gctcccgcac cctgtatggc
    18241 gcgtgcggcg accagcatgg gcagcgtatc ggcggcggcg gcaaggaagc tgcccgctgc
    18301 aaacacaatc agcccgacat aaatggtttt cttgcgcccg aacttgtcgg aagcgatgcc
    18361 caaaggcagt tgcagcagag cctgtgtcag cccgtaaatg cccattgcca gcccgaccag
    18421 cgttttgttg ccttccgcgc cgggcagcga ggcggcatac accgccaata cgggcagcac
    18481 gaggaacata cccagcatac gcagcgcgta cacgccggaa agcgtcgtac tggcgcgcca
    18541 ttcgtgcgga aacatttgga tgcggttatc tcttgccatc atattttttc agacggcatc
    18601 aacagttgca atgccgtctg aacttccagt gaacagattt tcggattata caggattcgc
    18661 cgtatttcgg ttgcggcgcg ggttcaaaat caacgccact gccagcggtt gcgccacgcg
    18721 cccaaaacgg cgttcggata tttattgctg cccaagctgc cgttaaagcg ggcaagcgcg
    18781 cggacgatgt tgcctttttc aagattccgg taatggcgca ggatggtaca gccgtaacgc
    18841 aggttggtgc ggatgtcgaa caggttgtgc gccggtttgc cgatgtagtt tttccaaaac
    18901 ggcataacct gcatcaggcc gcgcgcgccg acaccgctga ttgcatactg gcggaacgcg
    18961 ctttccacct caatcagccc caacacaatc tgcgtatcca aaccggcccg gctgctttcg
    19021 tactggatat tgaccagcag cctgcgccgc tcctcctcct cggggacgaa ccttgccaaa
    19081 cgtgccgaca tggcagacaa ccaacgttcg ccctctttcg gattgtcaaa caccagcctc
    19141 ggcggattga cgctgccgac agaactcctc atcacggaag ccacatcgtc ggcaagcgtt
    19201 tcctcacgtt gcgcgccggc gtgcgccaga ggactgagca acaacgcacc ggcggcacac
    19261 aacaggcggc ggcgttgcag attaacgggt agggtatcgg tcggtttttt catagggaac
    19321 gggggcgcgt ccggacgttt cagacggcat taaatattca aacagacata attgctttca
    19381 atgcgaaaaa ccgcgcacaa aatccaagcg cggcatatcg ccctgccctt ttcgggcaaa
    19441 ccttgatttt accgccctca agaacgcttg tccaaacagg cacaggcaac accacccggg
    19501 cattttccgt tttcaccggt tatccgtcgt ccggattatg cagcagcacc atcagcgcat
    19561 cacgcttttc gggcggcagc aggcggagac ctttgcaaa
//
LOCUS       DAWFKN010000033        19459 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFKN010000033 DAWFKN010000000
VERSION     DAWFKN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.033"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGR46_001135"
     CDS             complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGR46_001135"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0856084.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGR46_001136"
     CDS             complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGR46_001136"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0856085.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIEADMEAAKCW"
     gene            complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGR46_001137"
     CDS             complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGR46_001137"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0856086.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(6181..6501)
                     /locus_tag="WGR46_001138"
                     /pseudo
     CDS             complement(6181..6501)
                     /locus_tag="WGR46_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(6493..6642)
                     /locus_tag="WGR46_001139"
                     /pseudo
     CDS             complement(6493..6642)
                     /locus_tag="WGR46_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(6912..8003)
                     /gene="ychF"
                     /locus_tag="WGR46_001140"
     CDS             complement(6912..8003)
                     /gene="ychF"
                     /locus_tag="WGR46_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0856087.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEELAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEEDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(8117..9793)
                     /locus_tag="WGR46_001141"
     CDS             complement(8117..9793)
                     /locus_tag="WGR46_001141"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0856088.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLAHKVVNAIESQTNNFGFAYDVELGIKDKI
                     RVIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            9968..10399
                     /locus_tag="WGR46_001142"
     CDS             9968..10399
                     /locus_tag="WGR46_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0856089.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(10630..11325)
                     /locus_tag="WGR46_001143"
     CDS             complement(10630..11325)
                     /locus_tag="WGR46_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0856090.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(11341..11841)
                     /gene="hpaC"
                     /locus_tag="WGR46_001144"
     CDS             complement(11341..11841)
                     /gene="hpaC"
                     /locus_tag="WGR46_001144"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0856091.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALVHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(11956..12396)
                     /gene="nadR"
                     /locus_tag="WGR46_001145"
     CDS             complement(11956..12396)
                     /gene="nadR"
                     /locus_tag="WGR46_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0856092.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(12739..13248)
                     /locus_tag="WGR46_001146"
     CDS             complement(12739..13248)
                     /locus_tag="WGR46_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0856093.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(13327..13402)
                     /locus_tag="WGR46_001147"
     tRNA            complement(13327..13402)
                     /locus_tag="WGR46_001147"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13367..13369),aa:Lys,seq:ttt)
     gene            13613..14692
                     /gene="apbC"
                     /locus_tag="WGR46_001148"
     CDS             13613..14692
                     /gene="apbC"
                     /locus_tag="WGR46_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0856094.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGATAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            15875..19006
                     /locus_tag="WGR46_001149"
     CDS             15875..19006
                     /locus_tag="WGR46_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0856095.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAQAETHNYAIVMNER
                     NQLQVKQEGSYSTLREKDRKRKFIYTSQRNGAGQQNNFISFDNTDTLVSQQSGTAVFG
                     TATYLPPYGKVSGFDADGLQKRNNAVDWIHTTQAGLAGYAYTGVICRSNQCPQLVYET
                     KFSFDGIGLVKNAGSLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSEGTAKDGKVSNK
                     LVSSFDENNSNSNQNLVYTTKGHHISLGDWQREHTAMAYYLNAKLHLLDKKGIKDIAQ
                     GKTVDLGTLRPRVETTGRSWLDFWATWKIEDKGNITVRLGLPEVKAGRCINKPNPNPK
                     AQALSPALTAPALWFGPVQNGKVQMYSASVSTYPDSSSSQIFLQNLSRKDDTSKPGRY
                     SLKPLSTSEIKSKEPTFTGRQTVIRLDGGVQQIKLQGNEVTSFNVNNGNNTFGIVKDL
                     GVDPDASEWKKVLLPWTVRASNDDNQFKTINQQLNQQKIQYSQRYRIRDNGNRDLGDI
                     VNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRQYFDNDTSALK
                     DSTLAKELRTFAEKGYVGDRYGVDGGFVLRRITDDQDKQKHFFMFGAMGLGGRGAYAL
                     DLTKADSNNPTAVSLFDVKNGNNGKNSNNSNNSVQLGYTVGTPQIGKTHNGKYAAFLA
                     SGYATKDINNGDNKTALYVYDLESNNGTPIAKIEVPNGKGGLSSPTLVDKDLDGTVDI
                     AYAGDRGGSMYRFDLSGNNPTSWSARAIFSGNKPITSAPAISQLKDKRVVIFGTGSDL
                     SEDDVDNTDEQYIYGIFDDDTVANNVNVKLSGLGGGLLEQKLSEENKTLFLTDYKRSD
                     GSGDKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADGGKL
                     TKKSARPIVPEANTKVAQYSGHKQTAKGKSIPIGCMEKGNETVCPNGYVYDKPVNVRY
                     LDEKKTDGFSTTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDLDSLDITGPT
                     CGMKRISWREVFY"
BASE COUNT         4621 a         5277 c         5155 g         4406 t
ORIGIN      
        1 cactatagat tcccacttcc gtgggaatga cggttcagtt gcattccgac aacaccgtaa
       61 tcttgaaatc cgtcattccc gcgcaggcgg gaatccagac ccctgacgcg gcgggaatct
      121 atcggaaatg actgaaaccc cgagattcta gattcccact ttcgtgggaa tgacggttca
      181 gttgcgttcc gacaacaccg caatctcgaa atccgtcatt cccgcacagg cgggaatcca
      241 gacccacgac gcggcgggaa tctatcggaa atgactgaaa cctcgagatt ctagattccc
      301 actttcgtgg gaatgacggt tcagttgcat tccgacaaca ccgcaatctt gaaacccctc
      361 cgccgttata aagacaaatc acggcacaaa aaatgccgtc tgaaatgctg ttcggcggtt
      421 tcagacggca tttgctcaaa ctttatcagg cgtaatggcg cgtttcgcct tctccgccga
      481 cattctctgc acagcgtttg cagacggttt catagcctgc aaccgcgccc acatcgcggg
      541 tgtagtgcca gcagcgttcg catttttcac catcactggc tttagcggca acggcaagtt
      601 cgctgcctac tttcacttct gctttagaca ccagcaaagc aaagcgcaat tcttcgccca
      661 aagcattcag atagccggcc atttcttccg gcgcggtaat ttcggcttcg gcttgcaagg
      721 acgaaccgac ggttttgtcg gcgcgcaaag gctcgatggc ggcggttacc gcttcgcggg
      781 cttcgcggat tgccgtccat tttttcacca gttcggcttc ggctttttcg ttgatggtcg
      841 ggaactcgtg ccaggtgtgg aagaggacgc tgtcttcttc gccgccgccg atgatgtccc
      901 acgcttcttc gccggtaaag cacaaaatcg gcgcaatcaa gagaaccaaa ctgcgcgtga
      961 tgtggtacag ggcggtttgc gcgctgcggc gggcgcggct gtcggctttg gtggtgtaga
     1021 ggcggtcttt caggatatcg aggtagaacg cgcccaagtc ttccgagcag aaagaaacaa
     1081 tgtctttcac ggcaaaatgg aaggcataac gcggatagta atcgcctgcc agacgctctt
     1141 gcagccgacg cgccaatacc acggcgtagc ggtcgatttc caccatgtct gcctgttgca
     1201 cggcatcttc aataggattg aagtcgctga ggttggcaaa caggaagctc aaggtgttgc
     1261 ggatacggcg gtagctttcg gttacgcgct tgaggatttc tttggaaatc gccaattcgc
     1321 cgctgtaatc ggtggatgcc gcccacaggc gcaggatgtc cgcgccgaac tcgttataca
     1381 cttcttgcgg cgcgacgacg ttgccgatgg atttcgacat tttgcgcccg ttttgatcca
     1441 ccacgaaacc atgggtcagc agctgtttgt acggcgcgcg acccattgat gaggcgcagc
     1501 cggtcagcat ggacgactga aaccagcccc ggtgttgatc gctgccttcg aggtacaagt
     1561 cagcaggcca gtccaattct tcgcgctgtt tcacaacgga ataatgggtc gaaccggagt
     1621 cgaaccatac gtccatcgtg tcggagagtt tgtcgtaatg ttcgcaatct tccgcgctca
     1681 acaattcgcc cttatcgagg gagaaccaag cctcgatgcc tttttcttcg atgcgttgcg
     1741 cgactttttc caaaagttcg gcagagttcg gatgcagctc gcccgtttct ttgtgaacaa
     1801 agaaagtcat cggcgtgccc caatagcgtt ggcgcgaaac cacccagtcg gggcggcctt
     1861 caatcatggc ttccaaacgc gcacgacccc atgatgggaa aaattcagtg tcatcaacgg
     1921 ctttgatggc tttatcgcgc agggttttac catcggcacc tgctttatcc ataccgacaa
     1981 accattggcc ggtcgcacgg taaatcaacg gcgttttgtg acgccagcag tgcgcgtagc
     2041 tgtgttcgat tttgctgctt gccaagaggt tgccgatttc ttccaaccat tgcaggataa
     2101 cgggattggc ctcccaaacg ctcatgccgg caacgcgcgg cgtttcactg atgtatttgc
     2161 cttcggcgtt gacggggttg taaagctcga tgccgtattt attgcagacg gcgtagtctt
     2221 ccaaaccatg agcaggcgca gtatgcacca aaccggtacc ggcgtcggtg gtaacgtggt
     2281 cgccgttgag catgggaata tcgcgttcga ggaacggatg attcatgtgc aggttttcca
     2341 gcttgtcgcc ggtggtttcg gcaagaatag caatgccgtc tgaaaaaccg taacgtttga
     2401 gcgcgtcttc tgccaaatct ttcgccagca ccaatttgcc tttgggcgta tcgattaatt
     2461 gatacaccac gtccgcaccg gcagacacgg cttggctcgc tggcagcgtc caaggcgtgg
     2521 tcgtccagat gacggcaaac gctttgccct cgatgcctgc caaaccgaat gcagcggcaa
     2581 gcgcagcggt gtctttaaac ggatagccca cgtcaatcgc aggcgatact ttgtctttgt
     2641 attccacttc ggcttcggcc aatgaagagc cgcagtccaa gcagaactga accggtttcg
     2701 cgccgcggta gagatagccg gatttgtaga tttcgccgag catacgcacg gtatcggctt
     2761 cggttttaaa atccatggtc aggtagggct tgtcccagtc gcccaacacg cccagacgga
     2821 taaagtcttt tttctggcgg gcaacttgtt cggcggcata ttcgcggcac aattcgcgga
     2881 aacgcgcttt gggcatgtct ttgccgtgca gcttttctac catcacttcg ataggcaaac
     2941 cgtgacagtc ccaacccggc acataaggcg cgtcaaaacc ggcttgggtt ttgctgcgga
     3001 taatgatgtc tttgagaatt ttgttgacgg cgtgaccgat gtggatgtcg ccgttggcat
     3061 acggcgggcc gtcgtgcagg atgaacttcg gacgaccttt ggcgatttcg cgcagttttt
     3121 ggtagcgttt ttgctcgtac cagcttttca gccatgcagg ctcgcgcttg gcaagattgc
     3181 cgcgcatcgg aaacgggctt tcgagcaggt ttacggtttt gctgtaatcg gtcatttttt
     3241 aatctctatt gttacaatat ttcggttcag acggcattgc gcctcaaaca gtatttcaca
     3301 acgggaaacc cctatgccgt ctgaacagtt aaaaggttga ttgtagccca atcggatggt
     3361 ttgtataagg tttttctacc aacactttgc ggcttccata tcggcttcaa tctgcctttt
     3421 cagttctgcc ataccgtcaa acttttcctc atcgcgcagt ttgtgcagga agcggacgtt
     3481 caaccgttgt ccgtacaaat cgccttgaaa gtcgaacagg tggacttcaa gcttttgaga
     3541 acagccgcta tcaacggtgg gattgaaacc gaagctcgcc acgccgcgcc gcgtgccgaa
     3601 tgcgccgtcc gcttcgacga caaacacgcc gccgagtgca taacggtggc ggggcaggcg
     3661 gatgttggca gtcggggcgt ttaaggtgcg tccgagtttt ctgccgtgca ccaccctgcc
     3721 gctcaagacg tagtcgtgtc ccaaaagttt tttcgcatag gcaaggttgc cgtctgaaag
     3781 ggcttgtcgc acggcggtac tgctggtgcg gatgtcttcg acgatgacgg aaggcgtacg
     3841 ctcggtctgc atatcgggct gttgtgccaa aagttcaaaa cagccttccc gccccgcacc
     3901 gaaacggaaa tcgtcgccga cgagcaaata acgcgtattc aaggtttgac gcagcaggcg
     3961 gtcgataaac ccttgcgcgg atatttcgga aaaattttga tcgaaacgta aaacccagac
     4021 ggcatcgacg cagcccgtcc cttccagcaa ttccagtttg gtacgcaggg ggctgatgcg
     4081 gcagggcggg gttttgcctg cgagggcggc aaaaaattct ttgggttgag gttcgaagat
     4141 gacggcaacg acgggcagtc cgcgcgtatc ggcttcgagg cggagttttt ggaggatgtg
     4201 tttgtgtccg aggtgtacgc cgtcaaaatt gcctatggtt acggcggccc cgtgcggaaa
     4261 gtcgggcgcg ttgtgccgcc ccagcctgat tctcattgtt atgttcgggt atgtggtgaa
     4321 acaggcggtc attgtaaacg gtattgcggt ttatagacag tgtgccgccc gttacgcccg
     4381 cccgacgcgg gaaaagtagg caaatttccc gcagccgaac gcgccaaacg cataaagcgc
     4441 gcagcaggcg cggtgctatg tgttgaaaca tcgccccaaa cctgatgcaa catcagtgaa
     4501 aatcgttctt ttttaaccag gtaaaccgag acaaacaacc ctccgccgtc attcccgcgc
     4561 aggcgggaat ccggacctgt ccgcacagaa acttatcgga taaaaacagt tgctcaaacc
     4621 ccgagattct agattcccac tttcgtggga atgacggttc agttgcgttc cgacaacacc
     4681 gtaatcttga aactcgtcat tcccgctcag gcgggaatct agaacgtgga atctaagaaa
     4741 ccgttttgcc cgataagttt ccgtgcggac aggtccggat tcccgcctgc gcgggaatga
     4801 cggcatttct gcggcaatcg gattatttcc aaaccaaaag cgcgtgattg cgtttgccgc
     4861 gccgaaggat ggtgtatttg ccgaaacgtt tgtgttcgtc ggtcagcagg caggcatcgt
     4921 cggggcgttc ggcggcgtgg ttggggttgt tggcttcggc aggtttgccg ttgagcaaaa
     4981 ccgctttgct gttcacaaag ccgcgcgctt ctttattgga ggatgccaaa ccggttttga
     5041 ccaaggcttc gacgacattg atgccgtctg aaacttcaaa tgcaggcagg ccgtcgaggg
     5101 cgagctgctc gaagtcgctt tcggtcaggc tgctttggtc ttcggcaaac aggctttcgg
     5161 aaatgcgctg cgcggcggca agggcttctt cgccgtgaat caggcgggtc atttcttcgg
     5221 cgaggatgcg ttgcgcttcg ggcttgctgc cgcttgcctg gtctttggct tcgacggcat
     5281 cgatttcttc gatggacagg aaggtaaagt atttcaggaa tttatacaca tcggcatcgg
     5341 cgactttcag ccagaattgg tagaactgat agggcgaggt ttttttcgcg ttcagccata
     5401 ctgcgccgcc ttcggttttg ccgaatttgg tgccgtctga tttggtaacc aaaggcagag
     5461 tcagtccgaa cacttgtttt tggtacaggc ggcgggtcag gtcgataccg gcagtgatgt
     5521 taccccattg gtcggagccg ccgatttcca aaaccgcgcc gtggcgtttg ttcaactcgg
     5581 cgaagtcgta gccttgcagc agggagtagg cgaactcggt gaaggaaatg cctgcgccgt
     5641 cgcggtcaat gcgctgtttg acggattctt tgttcagcat ggcgttgacg gagaaatgct
     5701 tgccgatgtc gcgcaggaag tcgaggcagt tcattttgcc gaaccagtcg gcgttgttcg
     5761 ccataatggc ggcgtttccg ccttcaaagc tcaagaaagg ggttaattgg ctgcggatgc
     5821 tttccaccca gccggcaacg gtttcggcgg aattcaagct gcgttcggcg gctttgaagc
     5881 tggggtcgcc gatcataccg gtcgcaccgc ccaccagtgc aatcggcgta tgccccgcct
     5941 gttggaagcg gcgcaatgcc aatacgggca gcaggtgtcc gatgtgcagg ctgtcggcgg
     6001 tcgggtcgaa gccgcaataa agggaaattt tttgttcgtt caacaaagcg tctaaggctt
     6061 cgatgtcggt ggtttgcgcg ataaggccgc gcgattgcag gtcttggatg acgctcatcg
     6121 gtctctttca aaaaaaatta gcggttttgc aaaccgccga ttgtaacaaa tttaagcgaa
     6181 tcaatggtta tggcgcgtat cgagaaaccg ttgtttttcg gaaaaacgct tcgccaattc
     6241 cgtgccgccg taagggttga tgtggtcttt gtccgagtaa accggcaatc cgccgatttg
     6301 gaaatttgcg gggatatagg cggcggcatc aataatatag acgttggggt atttggctgc
     6361 caattccctg atgcgtgcat tggctttcag agtgctttcg tcgtccgggc gcagggtttg
     6421 gcggtaaccc ggtatgcgtg aagacaagat ataggcgcgc tggacgttgt aagacgaggc
     6481 aaggttgtcc gccatcaaaa agcctgaaat cacaaagaat atatcgacac cgagaaatcc
     6541 gcccggcagc caattttttt cgatatggaa cacgatgacg gacaaaacgg cggcggcgcg
     6601 caatgtgtcg atgtccgggc ggtagggtaa ggcttggctc ataatatttt tgctttcatc
     6661 gcggcggcgc aaatgccatc tgaaaggtca ttttgtatcg gggctcggga tattcggaat
     6721 gccggacgga tttttgcaac agcggggcag gcggtttgtt ttgcaggaat cggggagggc
     6781 aaatcggaaa tgcggacggg attttatttt gatgcagccg cattccggcg gtatggaaac
     6841 gccgaaaacc accaaaaccg gcttcagacg gcatttctgg caaagccgtc tgaaaacctg
     6901 ccgcatttgg gttacacgtt aaacaaaaag tgcatcacat ccccatcctg cacgacgtat
     6961 tccttacctt ccacacgcat tttgccggct tctttggctt tggcttcgcc gccgagcgag
     7021 acaaagtcgt cgtaagaaat gacttgggcg cggatgaagc cgcgttcaaa gtcggtgtga
     7081 atcacgccgg cggcttgtgg tgcggtgtcg cctttgtgga tggtccaggc gcggacttct
     7141 tttacgccgg cggtaaagta ggtttgcaag cccaagaggt cgtagccggc gcggatcagg
     7201 cggttcaggc ccggttcttc caagcccatt tcggcgagga attcggcttt ttcgtcctct
     7261 tccaattcgg cgatttcgct ttccattgcg gcgcatacag ccacaaccgg cgcgttttct
     7321 tttgcagcca attctttcag gcggtcgagg tgcgggttgt tttcaaaacc gtcttcggaa
     7381 acgttgccga catacatggc cggtttggca gtcagcagga acagcggctt gagcatggcg
     7441 agttcttctg cgtccaaacc gaaggagcgt acaggcttgc cttcgtccag atgaggcagc
     7501 agttttttgc acagatcgac cagtttttgt gcgtctttgt cgcctgagcg tgcgcgtttt
     7561 tcttcgcgga cgatggcttt ttcgacgctg gccaagtcgg caagcgccaa ctccgtaccg
     7621 atggtttcga tgtcggcaat cggatcgacg cggcctgcaa cgtggacgat gttgtcgtcg
     7681 tcaaagcagc gcacgacatt cacaatcgca tcggtttcgc ggatgttggc gaggaactgg
     7741 ttgcccaagc cttcgccttt gcttgcacct gcaaccaaac cggcgatgtc gacaaattcg
     7801 acgatggcag gctgcatttt ttgtggattg acgatttttg ccaattcagc catacgcgga
     7861 tcggggactt caacgatgcc gacgttgggt tcgatggtgc agaaaggata gtttgccgct
     7921 tcgatacccg attgggtcag cgcgttaaag agggtggatt tgccgacgtt gggcaaaccg
     7981 acgatgccgc atttcaaact catgtttttt cctgaaaata gagaaattta acggcggatt
     8041 atagcatacc gccgctcgcg ttccgaaaaa atgccgtctg aaacggcttc agacggcatc
     8101 cggtttcaaa aaccgctcag aacaagccgt gaatcacgcc gtgttcgtcc acatcgattt
     8161 tctcggcggc cggcactttc ggcagacccg gcatcttcat catattgccg cacagcgcga
     8221 cgatgaagcc cgcgccggcg gaaacggtga tgccgcgcac ggcgatgcgg aagccttcgg
     8281 ggcagcccaa cagtttggcg ttgtcgctca aagaatattg ggttttcgcc atgcaaatcg
     8341 gcattttgtc caagcccagt ttttccagcg aggcgatttc ggcagacgct tccgcgctga
     8401 aatcaacatc ttccgcgccg tacacttttt gggcaatcac acggattttg tctttgatgc
     8461 ccaactcgac atcgtaggcg aaaccgaagt tattggtttg actttcgatg gcgttgacga
     8521 ctttgtgcgc caaatccgcg ccgcccgcac cacctttgcc ccacacttcg gtcagggaaa
     8581 cttcaacgcc gtgttcggca caggcttttt caatcatcgc caactcggca tcggagtcgg
     8641 acacgaagcg gttgagcgca acgacgacgg gcagtccgaa tacgtttttc aggttggaaa
     8701 tgtgtttcaa gagattaggc aggccttttg ccaaggcttc gaggttttct tcgccgaggt
     8761 tggcgcgttc cacgccgccg ttgtatttca aagcgcggac agtggcgacg acaacagccg
     8821 catcaggttt caaatccgcc agacggcatt tgatgtcgca gaatttttcc gcgcccaaat
     8881 ctgcgccgaa gcctgcttcg gttacggcat aatcggcaag atgtttcgcc aaacgggtgg
     8941 cggttacgga gttgcagccg tgggcgatgt tggcgaacgg gcctccgtgt acgaaggcgg
     9001 gcgtgccttc gatggtttgc accaagttgg gcttaatcgc atctttaagc aatgccgcca
     9061 tcgcgccgtg tgccttcaaa tctttggcat aaacggggct gccgtctttg gcgtaggcga
     9121 cgaggatgtt gcccaaacgc tctttcaaat cgctgatgtc tttggcaaga cagaataccg
     9181 ccatcacttc ggaagcaacg gtaatgtcga aaccgtcggg gcgcatcacg ccgtcaacag
     9241 gcttgcccat gccgtcgatg atgttgcgca actggcggtc gttcatatcg accacgcgcc
     9301 gccacaggac gcgtttcgga tcgatgttca actcgttgcc ttggtagata tggttgtcga
     9361 gcatggcggc gagcaggtta ttcgccgcac cgatggcgtg aaagtcgcct gtgaagtgca
     9421 ggttgatgtc ttccatcggc aaaacttggg catagccgcc gcctgccgca ccgcctttca
     9481 cgccgaacac cggacccaaa gaaggctcgc gcaaagcaat cacagagtct ttgccgatat
     9541 ggcgcaacgc gtccgccaaa ccgatggtta cagtggtttt accctcaccg gcaggagtcg
     9601 gattaatggc ggtaaccaaa atcagacggc cttgtttttg cggaagcttg aatgcctcgg
     9661 caggattgat tttggctttg taatggccgt agggttcgat attgtccacg ttcaaaccca
     9721 gcttggcggc aatttcacca atcggacgca tggtggagga ttgggcgatt tcggcatcgg
     9781 ttttgaagct catgattttc ctttagaaat gaggagggac atgccgtctg aaagcatcag
     9841 gcgacaaaca ggtggattga aaataatatc aggcatatta taacgttatc cgcaccaaac
     9901 ccgcagtgaa atttttgacg ctgcaacaaa aataccgttc atattgttca caatccaagg
     9961 agaaaacatg agcagcaacg catggctgtt ttgggcattg gcatcggcag gcttcgcctc
    10021 attgaccgcc atcttcgcca aaatgggttt acagggtata gattcagatt tcgctacctt
    10081 catccgcacc ttggtcatcc tcgccgcttt gttattgttt ttgacctaca ccggcaaatg
    10141 gcagggtgtg aacggcttta cggggcacaa ctggacattc cttatcctat ccggccttgc
    10201 taccggcgca tcttggcttg cctacttcaa agccctgcaa ctgggtaacg cctcgcaagt
    10261 cgcccccgtc gacaaattca gcctggtctt ggtcgcgctg atggcggtgg ttttcttgga
    10321 cgaacgcccg aacacgcagg aatggatagg cttggggctg gtaacggcgg gcgtgttggt
    10381 gctggcgttg aaacgttaaa ccgaatccgc cataccgcct gaaaccgggt tttgacaatc
    10441 tgacgggcgg cggtctgaac aaagttaccc aataaaatcc gacaccgcgc cgacggcaaa
    10501 ccgccgcgct tcagatggca tttgttggac ggaaagcccc gaacgcccta ttttcacgcc
    10561 tccacgaaaa tatgctggac atccggtcgg aaccgttcgg cagccggctc ggcaagccac
    10621 cgttttattt cacttccaag cccctgcaag ggcttgagcc tctttcagac ggcataccgt
    10681 gccgacatcc agccacaagc ccgtatgctt ctgaccgctc acgcggtttt gccgcatttc
    10741 gccacgcaat acgggcgcga gtttcgccac actgcccgct tcgattccgt caaacatttc
    10801 aggacggtaa atacccacgc cgctaaatgt caatccgttg ccgccattta cttccggccg
    10861 cacgctgccg tcgggcagca gggaaaaatc gccgtcgggg ttgtgcggcg gattttccac
    10921 cagccacaga tgggcggaaa tatgttccgg cagggacgat gccgtctgaa acgcggcggt
    10981 aaaatcgatg tcggtcagca cgtcgccgtt gaccaccaaa aacggctgcc cacccaacag
    11041 cggcaatgcc tgcgcgatgc cgcctgccgt ttccaaaccg cctgcgggtt cgggcgaata
    11101 ggcgatgttc acgccataag ccgagccgtc gcccaaagca tcttctatct gccgacccag
    11161 ccaagcgtgg ttgatgacga tttcggtaaa ccccgcctgc ttcagacggc ataggtgcca
    11221 accgattaga ggcttacccg ccacatcgag cagcggcttc ggagtggtat cggtcaaagg
    11281 gcgcatacgc tcgccgcgtc ctgccgccag tatcatcgct ttcatatatc tgtccgaata
    11341 tcagtctaaa aatctaaact gccgtctgaa atacagcagc gcggggcgtt tgcacccgca
    11401 gtttttgatt tcgtcgagcc tgacgtaaaa cacaaaatgc gtgccgattt catgtttgcc
    11461 gacaatatgc ccgtgcaggt gcaccaacgc gccctctatt tcaagttgtc ccgttttgcc
    11521 gcgatgccag atgtggtagg caaaccgctc ttcgggcgac aggccggtca gcccggcaaa
    11581 atgttcggca acatcctgat gttcgtccgc cagcgtattg atgcacaggc tgccgttttc
    11641 cgacaggatc ggaatgatac gggctttacg gttgatgcac agcatcacgg tcggcggctc
    11701 gtcggtaacc ggcgcgaccg ccgtcattgt aatgccgtaa cgccctgccg caccgtctgt
    11761 cgtgatgaca tgaacgcctg ccgcacagga tgccatcgca tcacggaacg aagtttgaaa
    11821 agttttctgc aaatccgcca tttttcccct ttaaactgtc ccctatataa gaatgctgca
    11881 cacaaggcac cccccatgtg cagcagttct gattcaaaaa gccgtcggtc ggacatttcc
    11941 gcgcgttacg gcgtattacg agttcaacgc atcctcgatt ttggcaagtt ctgccaacag
    12001 gtctttaagc agcagcattt tctcgcggcc cagcacttcc tcgatagcgt cgtaacgctc
    12061 gtccacttct tcgccgattt cctcatacag cttctcgccc tcggcagtca gcttcagaaa
    12121 aacacgtcgt tggtcgttgg aaggtttcag gcggacgacc aaacccgctt tttcaaggcg
    12181 ggtcaggata ccggtcaggc tggggcgcag aatacacgcc tgattcgcca aatcttgaaa
    12241 gtccagcgtg ccgttttccg ccaaaagacg gataatccgc cattgctgat cggtaatatt
    12301 cgcctgattc agaataggcc tgaattgggt catcagggct tcccttgcct gtatcagact
    12361 gatattgata gacgcatgtt ttgattgggt aggcattgtt caagtctcca agttatcgaa
    12421 aatcaaactt tcaaaccgtc gggaaagcct gtgggcgtaa attttgatgc aaccgttata
    12481 taacaaaacg aacatatagc aacaatacgc tataaaccgc atcggacgac tgggtataaa
    12541 agactttaat tccgataatc ctatctaaaa atattttaat agttatatct tagtctattt
    12601 tccctacaat ctcaacaaag tattacattt actgtcgaaa gaactttctt ccagaaaaaa
    12661 caaaagccgc cgcatcagac gagcaaggta tatgccgttt gattctctac atagcgtaaa
    12721 gtttaacaaa aacaaaagtt aaccgaaaac atccgcctga aaaattcgtg cgcgcaagcc
    12781 ccaataactg ctgattcccg tcgtataatg gaccattttc ccatttttga ccaaaacgac
    12841 ggcaggcgtt gcgacaatcc gccaagacct tgccaaaccc ccgtcctcat cgttgacagt
    12901 cggaaagccc aagccgcgtt ttgccatata cgccgccact tccgccgaac tgccggaacg
    12961 taccgccacg ccgacgaccg gcacgccgtc cgccgccaaa tcatcgatta tcggcgactg
    13021 ataacggcac acgccgcacc agctccccca aaaatacacc agcaccgtcc tatcccggct
    13081 aaactgtccc aaagtcagcc gctgccccga cagcagggtc aaaggccgcc ctgccgcgcc
    13141 ggcaggttcg tcgggcttgc gtacccaatc caaaaacagc gacaccaata aaaacaccaa
    13201 tgccgtctga acggcaaatt tgatgcccga aagcagtttc tttttcatac gctctctcaa
    13261 acggtacgcc cgcgcaaccg gcaggcaaac aaaaagccaa gtctcaaaac ttggcttccg
    13321 gttatctggt gggtcgtgag cgattcgaac gctcgaccaa cggattaaaa gtccgctgct
    13381 ctaccgactg agctaacgac ccgataagcc gtgcattata cagcaccatc ctacctcgtc
    13441 aagcaaattt tacaggctta attgcagacc actgtttgca cgggatattt tgacaacgga
    13501 ttttcacaat ccgccgcata ccgtgtaaaa gttcgcacaa ggaaaagcaa accgcccgaa
    13561 atcaatgtac actttccgcc cgtttcccaa actgcacaca gaaacacaca ttatgaacat
    13621 acaaaacatc cgcaccctcc tcgacaccgt cgccgttccg aatacggcac gcacgctcgg
    13681 cggcgaaaag gccgtccgtt cggtcgaaca gcgttcagac ggcatccata tcgccctgca
    13741 tttcggcttc cctgtcgcgc acatcgccgc cgccgtcgcc gattccgtac aagaaatcct
    13801 gatgcccgaa acaggcggcg cgcacatcca tctgtccata gacaccgaaa tcggcacaca
    13861 taaagtccaa cctggcgtta ccaccatcaa aggtgtgaaa aacatcattg ccgtcgcctc
    13921 cggcaaaggc ggcgtgggca aatcaacaac caccgccaac cttgccgccg caatggcgcg
    13981 gatgggcgcg cgcgtcggcg tgctcgatgc cgacctttac ggcccgagcc aaccgaccat
    14041 gttgggtgtg gacgaccgca aacccgatca gaaaaaccaa aaactcattc ccgtcgaatc
    14101 ttcagacggc atacaggtca tgtctatcgg ctttctcgtc gataccgacc aagccgtcgt
    14161 ctggcgcggg ccgatggtca gccaagcctt gcagcaactg atgtttcaaa gcgagtggga
    14221 cgaagtggac tacctgttta tcgacctgcc ccccggcacg ggcgacatcc aactcacgct
    14281 gtcccaacgc atccccgtaa ccggatcggt catcgtaacc acgccgcagg acatcgccct
    14341 gatagacgcg cgcaaagccg tggatatgtt ccgaaaagtc aacattccca ttttaggtgt
    14401 attggaaaat atgtccgtcc acatctgctc caactgcgga cacagcgaag cactgttcgg
    14461 cacagacggc ggcaaagact tggcggcacg cctcaacgtc cccctgctgg gacagcttcc
    14521 attaagcctg cccgtgcgcg aagccatgga cggaggggcg acggcgcaac tgttcgacga
    14581 acaccccgcc atcgcccgaa tctacaccga tgccgcattc caaatcgccc tgggcattgc
    14641 cgacaaaggc aaagacttca gcagccggtt ccctaaaatc gtcgtcgaat aaagccgcgt
    14701 ccgaaaccgc aacagcaatg ccgtcccaag ccccgcgcct gccgatatgc cgtcattccc
    14761 gcgcaggcgg gaatctagaa cgtggaatct aagaaaccgt gttgtaaagg cagaccgatg
    14821 ccgtcattcc cacgaaagtg ggaatctaga acgtaaaatc tcaagaaacc gttatacccg
    14881 ataagtttct gcaccgacag gtctggattc ccgcctgcgc gggaatgacg gttcgggtat
    14941 ttctgacgat ttaggcattc ccgacaaggt ggattttcaa ggtgttgtat agggtgtagg
    15001 aggattcgta aaaggtgagt tatagggtgg gcttcagccc accgattcca acgattccac
    15061 caatcctaca ccgttcccat agactcaaat caacacagaa acttatgcgc cgtcattccc
    15121 gcgcaggcgg gaatctagac cttagaacaa cagcaatatt caaagattat aaaagacccg
    15181 tcattcccgc gtaggcggga atctagacca ttggacagcg gcaatattca aagattatct
    15241 gaaagtccga gattctagat tcccactttc gtgggaatga cgcggtgcag gtttccgtgc
    15301 ggatggattc gtcattcccg cgcaggcggg aatccagacc ttgggataac ggcaatattc
    15361 aaaggttata aaagacccgt cattcccgcg caggcgggaa tctagacctt agaacaacag
    15421 taatattcaa agattataaa agacccgtca ttcccgcgca ggcgggaatc tagaacgtaa
    15481 aatctaaaga aaccgtgttg taacggcaga ccgatgccgt cattcccgcg caggcgggaa
    15541 tccagactgt cgggcatctg cagcggtttg ctaaaaaacg ctttaccgtg atcagtgtgc
    15601 aaagttaaaa tggggaggta agctttccaa ccagcaattc ggcgggcgcg ggatcgggcg
    15661 gtttgccgaa tcccgccgtt tgcggcgcgc ctgccgccga cggtatcccg cgaagcaaga
    15721 tttaagggat aaaatatgtt ccaacacgca gggcggcaca taaggcgccg ccctgattcg
    15781 gaagggcttg cacccctccc ggacaaagcc tgatcctgcc gccccgaagg acggatgccc
    15841 gaagggcggg gggtttgacc gaaaaggaaa tacgatgaat aaaactttaa aaaggcgggt
    15901 tttccgccat accgcgcttt atgccgccat cttgatgttt tcccataccg gcgggggggg
    15961 ggcgcaggcg gaaacccata actacgctat tgtcatgaac gaacgaaatc agctgcaggt
    16021 aaagcaggag ggttcatatt caaccttaag ggaaaaagac aggaaacgca aatttatcta
    16081 tacgagccag agaaacgggg cgggccaaca aaacaatttt atctcattcg acaataccga
    16141 tacccttgtt tcccaacaaa gcggtactgc cgtttttggc acagccacct acctgccgcc
    16201 ctacggcaag gtttccggtt ttgatgccga cgggctgcaa aagcgcaaca atgccgtcga
    16261 ttggattcat accacccagg ccgggctggc aggctacgcc tacaccggcg tcatatgcag
    16321 aagcaaccaa tgcccccaac ttgtctatga gaccaaattt tccttcgacg gcatcggttt
    16381 ggtaaaaaat gcgggcagcc tggataggca cccggaccca agccgcgaaa attcgcccat
    16441 ttacaaattg aaagatcatc catggttggg cgtatctttc aatttgggca gcgagggtac
    16501 cgccaaagat ggcaaggtat ccaacaaatt ggtatcttct tttgatgaaa acaatagtaa
    16561 tagtaatcaa aacctcgtct atacgacaaa aggccaccat atttcccttg gcgactggca
    16621 gcgcgaacat accgccatgg cctattatct gaacgccaaa ctgcacctgc tggacaaaaa
    16681 agggattaaa gatatcgccc aaggcaaaac agtggatttg ggcaccttga gaccgcgcgt
    16741 cgagacgaca ggacgaagct ggctagattt ttgggctacg tggaagattg aagataaagg
    16801 gaacattaca gtccgcctcg gcctgccgga agtcaaagca ggccgctgca tcaacaaacc
    16861 gaaccccaat cccaaagccc aagccctttc gccggcactg actgcccccg cgctgtggtt
    16921 cggacctgtg caaaatggca aggtgcagat gtattccgct tcggtttcca cctaccccga
    16981 cagttcgagc agccaaattt tcctgcaaaa cctttcccgc aaggatgaca caagcaaacc
    17041 gggccgctat tccctcaaac ccttgagtac gtcggagatt aaaagtaaag agccgacttt
    17101 cacagggcgg caaaccgtca tccgattgga tggcggcgta cagcagatta aacttcaagg
    17161 caatgaggtc actagtttta atgtaaacaa cggcaacaac actttcggca ttgttaagga
    17221 cttgggcgtc gatcctgatg ccagcgagtg gaaaaaagtg ctgctgcctt ggacggttcg
    17281 tgcttccaat gatgacaatc aatttaaaac aattaaccaa caacttaacc aacaaaaaat
    17341 ccaatacagc caaagatacc gcatccgcga caacggcaat cgcgatttgg gcgacatcgt
    17401 caacagcccc atcgtggcgg tcggcgagta tttggctact tccgccaacg acgggatggt
    17461 gcatatcttc aaacaaagcg gcggggacaa gcgcagctac aatctgaagc tcagctacat
    17521 ccccggcacg atgccgcgcc aatattttga taacgacact tccgctctca aagactccac
    17581 cctcgccaaa gagctgcgca cctttgccga aaaaggctat gtgggcgacc gctacggcgt
    17641 ggacggcggc tttgtcttgc gccgcattac agatgaccaa gacaagcaaa aacatttctt
    17701 tatgtttggt gcgatgggcc tgggcggcag aggcgcgtat gccttggatt tgaccaaagc
    17761 cgacagcaat aacccgaccg ccgtttccct gtttgatgtc aaaaatggca ataatggcaa
    17821 gaatagcaat aatagcaata atagcgtgca attaggctac accgtcggca cgccgcaaat
    17881 cggcaaaacc cacaacggca aatacgccgc cttcctcgcc tccggttatg cgactaaaga
    17941 cattaacaac ggcgacaata aaaccgcgct gtatgtgtat gatttggaaa gcaacaacgg
    18001 tacgccgatt gcaaaaatcg aagtacccaa cggcaagggc gggctttcgt cccccacgct
    18061 ggtggataaa gatttggacg gcacggttga tatcgcctat gccggcgatc gcggcggcag
    18121 tatgtaccgc tttgatttaa gcggcaacaa cccgaccagt tggtctgcac gcgccatttt
    18181 cagcggcaac aaaccgatta cttccgcgcc cgccatttcc caactgaaag acaaacgcgt
    18241 ggttatcttc ggcacgggca gcgatttgag tgaagatgat gtggacaata cggacgaaca
    18301 atatatttac ggtatctttg acgacgatac ggtggcgaat aacgtaaatg taaaactcag
    18361 cggtttggga ggcgggctgc tcgagcaaaa gcttagtgag gaaaataaaa ccttattcct
    18421 gaccgattat aagcgatccg acggctcggg cgacaagggc tgggtagtga aattgaagga
    18481 cggacagcgc gttaccgtca aaccgaccgt ggtattgcgt accgcctttg taaccatcca
    18541 taaatatacg ggtacggaca aatgcggcgc ggaaaccgcc attttgggca tcaataccgc
    18601 cgacggcggc aagctgacca agaaaagcgc gcgcccgatt gtgccggaag ccaatacgaa
    18661 ggtcgcgcaa tattccggcc ataagcaaac cgccaaaggc aaatccatcc ctataggttg
    18721 tatggaaaaa ggcaatgaaa ccgtctgccc gaacggatat gtttacgaca aaccggttaa
    18781 tgtgcgttat ctggatgaaa agaaaacaga cggattttca acaacggcag acggcgatgc
    18841 gggcggcagc ggaacattca aagagggtaa aaaacccgcc cgcaataacc ggtgcttctc
    18901 cggaaaaggt gtgcgcaccc tgctgatgaa cgatttggac agcttggaca ttaccggccc
    18961 gacgtgcggt atgaaacgaa tcagctggcg tgaagtcttc tactgatttg cacgcgaaaa
    19021 tgccgtccga aaggttttcg gacggcattt tttgcgtttt tcgggagggg cgggttcgta
    19081 aaaggcgggc tatagggtag gcttcatctc gccaatctca ctgaatccat caatttccac
    19141 aattcaatta aataccgtca aaccgatgcc gtcattcccg cgcaggcggg aatctagacc
    19201 ttagaacaac agtaatattc aaaggttagc tgaagcttta gagattctgg attcccactt
    19261 tcgtgggaat gacgggattt gagattgcga catttatcgg gagcaacaga aaccgctctg
    19321 ccgtcattcc cgcgcaggcg ggaatctaga ccttagaaca acagcaatat tcaaagatta
    19381 tctgaaagtc cgggattctg gattcccact tccgtgggaa tgacgggatt agagtttcaa
    19441 aatttattct aaatagctg
//
LOCUS       DAWFKN010000034        18798 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFKN010000034 DAWFKN010000000
VERSION     DAWFKN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.034"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR46_001150"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR46_001150"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0856096.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTSGLAGMAKG
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WGR46_001151"
     CDS             complement(1906..2343)
                     /locus_tag="WGR46_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856097.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGR46_001152"
     CDS             complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGR46_001152"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0856098.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAQAAAPAPAAAPAPTAVAAPAPSAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGR46_001153"
     CDS             complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGR46_001153"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0856099.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGGGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEAERFNRLNPLETAKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRSFFEVDRYNVAVAALSA
                     LADQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7532..9244
                     /locus_tag="WGR46_001154"
     CDS             7532..9244
                     /locus_tag="WGR46_001154"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0856100.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGSVGFAGKVYADFVTEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDAVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNA"
     gene            9307..9963
                     /locus_tag="WGR46_001155"
     CDS             9307..9963
                     /locus_tag="WGR46_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0856101.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCCRKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGR46_001156"
     CDS             complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGR46_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0856102.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10421..10969)
                     /locus_tag="WGR46_001157"
     CDS             complement(10421..10969)
                     /locus_tag="WGR46_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0856103.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10971..11531)
                     /locus_tag="WGR46_001158"
     CDS             complement(10971..11531)
                     /locus_tag="WGR46_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0856104.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11859..13664
                     /locus_tag="WGR46_001159"
     CDS             11859..13664
                     /locus_tag="WGR46_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0856105.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13784..15268
                     /locus_tag="WGR46_001160"
     CDS             13784..15268
                     /locus_tag="WGR46_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0856106.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSSIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15597..17624
                     /gene="uvrB"
                     /locus_tag="WGR46_001161"
     CDS             15597..17624
                     /gene="uvrB"
                     /locus_tag="WGR46_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0856107.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            17692..18456
                     /locus_tag="WGR46_001162"
                     /pseudo
     CDS             17692..18456
                     /locus_tag="WGR46_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245990.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(18621..>18798)
                     /locus_tag="WGR46_001163"
     CDS             complement(18621..>18798)
                     /locus_tag="WGR46_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856108.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYN
                     GIDCKSFPLFLKEC"
BASE COUNT         4709 a         4673 c         4983 g         4433 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccaccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgttcgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctca actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtctactt tgcggccttt agccatgcct gccaaaccgc
     1261 tggtcagacg ggaaaccacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggggca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ctgcttcggc tttaggggcg gcagccgcac cttcggcttc gacgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agactgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaacagc ttcgccaaga atacggtgaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca acgggcacat caggcgcggg gcaaactctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 cggcgtgaaa cctgtgccgc cgatgccgcc caagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcgcg ggcttttttg gacaattcgg tcaattcttg
     2761 gctgatttgt ttcaagcctt tttgatctac gtctttgatg acgggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaggt tgtcgccatc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccagagga gacagtttca cgccttcgcg ttcccattct ttgttcagtt gtttgcggaa
     3001 ttcttccagt tcggtcatat ccgcttcttc gtgtacggta acgtgcggaa tcacaaccca
     3061 gttgcgggac aggttttgac cggaaatttt cttaatgcgg gacaattctt taacttcgac
     3121 attaccgaat ttggagaagt ccactttagg ccagggcagc aagtccagac cgccgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc accgccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcga cagcagtcgg
     3421 agccggggcg gcagccggtg cgggtgcggc ggcttgagca ggggctgctg cggcagagcc
     3481 ggcggtttct acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     3541 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     3601 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc actttgattt caacggcgat
     3661 tacatccaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     3721 tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag gagcggcttc
     3781 ggcagcggcg gcaccggttt caacggtcag aataacgccg ccttcagaga ttttatcgcc
     3841 cactttaact ttaacttctt taacaacacc ggctgcatca gcaggtacat ccatagtggc
     3901 tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca atagtgtcgc ctactttgac
     3961 ttctacggcg atgatgtcga cgttttcatg accgccgata tcagggactt tgatttctac
     4021 gatactcatt tgagttcctt taatattttc agtttgatgc cgtctgaaaa tttgtttcac
     4081 acggcatcgg tacagtttgt tcaaaccatt caagcattcg gttttcagac ggcctgatgt
     4141 ctgaatgaag caaacaggcc gtctgaaaca tcaattagcg tttccagcta ggagctgaat
     4201 cggctttgat accatatttt tcaatggctt gttgaacggt ttctttgctg actttgcctt
     4261 gatcggccaa tgcgctcaat gcagcaacgg caacgttgta gcggtcaact tcgaagaagc
     4321 tacgcaggtt ggcgcggctg tcggagcggc cgaagccgtc agtgcccaag acgtggtaat
     4381 cgttcgggat ataggcgcgg atacggtcgg catagctgcg gatatagtcg gtagcggcaa
     4441 tcactgggcc gtcatgacct tgcagttgag aagtaacaaa cggtactttt gcagtttcta
     4501 aaggattcag gcggttgaaa cgttctgctt cgacagcgtc gcgatgcaac aggttgaaag
     4561 atgggcaaga ccaaatgtct gcttccacac cgaagtcggc tttcagcagc tcggcaccgg
     4621 caatcacttc ttgcagaatc gtaccggaac ccatcagttg gactttcttg tcgcctttgc
     4681 cgccggcttt cagcaggtac atacctttga ggatgtcttg ttccgcacct tccggcatat
     4741 ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct tcattattgg
     4801 catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg aaagtcgggt
     4861 cataagatac gcagttcgga atcaaatctg cttgaatatg gctgtggccg tcttcgtgtt
     4921 gcagaccttc gccgttcagc gtcgtacggc cggcagtacc acccaacagg aagccgcgtg
     4981 cgtgcatatc acctgccgcc caagccaagt cgccgatacg ttggaaaccg aacatagagt
     5041 agtagatgta gaaaggaatc atcgcaaagt tgctgttggc gtagctggtt gcagcagcga
     5101 tccagtcagc cattgcgccc ggttcgttaa taccttcttg caagatttga ccgtcaacgg
     5161 attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga cctttcggat
     5221 tccaaatacc gtattggcgg aacatgcctt ccataccgaa agtacggctt tcgtcgggaa
     5281 cgataggtac gacgcgtttg ccgatttttt tgtctttcaa caaggttgac aaaatgcgga
     5341 caaatgccat agtggttgag aattcgcggt cgccgctgga tttcagttgg gtatcaaatg
     5401 cggacagctc cggcacttcc aaaacttctt gggtaggttt gcgttgcggc aggtagccgc
     5461 ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca
     5521 ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc
     5581 ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg ttctgacctt
     5641 cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg gtaggtttgc
     5701 cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg tcgtggccgc
     5761 cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgct ttcagttcgg
     5821 gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt
     5881 cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct ttcgccaaga
     5941 ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg ccggcaaagt
     6001 tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc aagcgttgca
     6061 gattgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc agtgcgattg
     6121 caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat actttacggc
     6181 ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc tgataaatcg
     6241 ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg ggcaagaggt
     6301 gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag ttgttcagct
     6361 ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg gcgacgtgac
     6421 cttggaagaa gaccaaatcg ccttcttcgc ctccgccttt ggctttccaa aagtggttga
     6481 aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca cccagttcca
     6541 aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgga
     6601 tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc gaaacggtat
     6661 tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg cagtatttga
     6721 ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg acggagctta
     6781 acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg gtggacatag
     6841 tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac ccctttattt
     6901 tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga aaaaaataat
     6961 ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa acttgaaaaa
     7021 aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg caaataacca
     7081 tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag tctttttgat
     7141 ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat atcgaatcat
     7201 tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt tgtggataac
     7261 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
     7321 tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt caatatgatg
     7381 tttctggtca tgtttttgcc gtctgttatg ggatttgtcc cgcgcttggg gcagggggtg
     7441 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
     7501 tttgaatctg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
     7561 tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga ttcgcgccgg
     7621 cctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc tgcgcgtgtt
     7681 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagttgct
     7741 gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg agttttacgg
     7801 taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg gcccgacctg
     7861 cgaggaagtc atcgccgaca tcgtgcgcaa agaaatcaac agctacaaac agctgccgaa
     7921 aaacttttac cacatccaaa ccaaattccg cgacgaagtg cgcccgcgtt tcggcgtgat
     7981 gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc catgccgact acgcctcgct
     8041 tcagacgacc tatgatgcca tgtatgacgc ttactgccgc atcttcactc gtttgggttt
     8101 ggcattccgc cccgtcgccg cagacaccgg cagcatcggc ggtacgggtt cgcacgagtt
     8161 tcaagtgttg gcggaaagcg gcgaagatgt tattgcgtac agcgacactt ccgattacgc
     8221 cgccaatatc gagttggcac cgaccttgcc gcttaaaggc gaacgcgccg ctgctcaggc
     8281 tgtgttgacc aaagtacata caccaaacgt caaaaccatt gagtctttgg ttgaattcct
     8341 gaatattccg gttgaacaaa ccctcaaatc catcgtggtc gaaggcgaaa acgaaggcga
     8401 aatcgtctta ttactgttgc gcggcgacca tgagttcaac gacatcaagg cagaaaaact
     8461 ggcgggcgtg aaatcgccat tgacgatggc aagttctgcc gcgattgtcg aacagttcgg
     8521 cgcaaacggc ggctcgctgg gttcggtcgg ctttgcaggc aaagtttatg ccgatttcgt
     8581 cactgaaaaa ggcgcggact gggtcatcgg tgcaaacgaa gacggctacc actacaccgg
     8641 cttcaacttc gggcgcgacg ctgccgagcc tgaatttgtc gatttgcgca atgtcgtcga
     8701 aggtgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
     8761 acacgtcttc cagttgcgcg acaaatacac ccaagccatg aacgtgagct tcctcgacaa
     8821 caacggcaaa tcgcaaatca tggaaatggg ctgctacggt atcggcatca cccgcgtcgt
     8881 tgccgccgcc atcgagcaga acaacgacga aaaaggcatc atctggacga aggcgatggc
     8941 gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacgccg tgcgcgaagc
     9001 cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggatgaccg
     9061 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg ttgggtattc cgcaccgcat
     9121 cgtcatcggc gaccgcgctt tgaaagaagg caatgtcgaa tacgccgaac gccgcaacaa
     9181 cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt gtaacagctt cattaaatgc
     9241 gtaagcttgc tcgaatgccg tctgaaaata atttcagacg gcatttttac ttagggggaa
     9301 agtgaagtgg cttcagtatt tttagaagga gaggcagtcg aggtcggcaa tattttctgt
     9361 atcggcagga actatgccgc acatatcgaa gagttaaaaa acgaaatacc ttccgaacct
     9421 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
     9481 gagtttagta gggatgtgca gtttgagtgc gaactcgtgc tgctggtcgg taaggattca
     9541 gacggcacgg gcgagggtaa agatattttg ggatgtgtcg caggatatgg tgtagggctg
     9601 gaccttacgg cacgggatat ccaatgctgt cggaaggaaa aagggctgcc ctggctgaag
     9661 gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg cagaatcggc
     9721 aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggat
     9781 accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc agattacggt
     9841 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
     9901 ggggacaatt tggcattgga gctggacggc ttggtgcgcg cctctttcac ggtagggtgt
     9961 taaccgcgcc gcttaaccct cacgcccgtt gaaacattcc gccggaccgc cctcgaaaaa
    10021 accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgccgaa
    10081 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    10141 atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg acaggtgcgt
    10201 catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat aacgcggctg
    10261 ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc cgccggtaac
    10321 ggtgctcaaa ggatgggata gccctaattc cgcgtctcct tgtgccacgc cgatacgcgc
    10381 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    10441 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    10501 aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt cagccacgca ttgtcggcaa
    10561 gttcgcgttc gagctggcct tttccccagc ttgaatagcc tatgctgatc aaggctttgt
    10621 caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtt agcgcgatat
    10681 tgtctgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg acataacctc
    10741 gctcgacttg caccggcccg cccatcatca cgctgtcgtg ctgcatccgc atggggatgt
    10801 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    10861 tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    10921 cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata tcagcgtttg
    10981 tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt gccggaagta
    11041 tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac cgcgcttaag
    11101 gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc gaaggttttg
    11161 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct cgcaccgtgt
    11221 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    11281 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    11341 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    11401 ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact tgcccgtccg
    11461 attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga cagcagtagc
    11521 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa ggcggcaggg
    11581 cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt atgcggctta
    11641 atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat atgacgcaca
    11701 atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa gattgggcaa
    11761 acggcatttt cagacggcat aactgacagt ataatccaaa cgtccggctg ctttcgtgca
    11821 gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa ccccttctgc
    11881 ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg gcacacaacc
    11941 gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa gcggcggaag
    12001 gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc ggcaaagagg
    12061 cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa gcagagaagg
    12121 aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa tcgaaggcgg
    12181 aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac gcaaaaacac
    12241 agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca gggcaatccg cgtaagggcg
    12301 gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac aagaacggca
    12361 aagcagtgaa acaggataaa aaatacaggg aagagaaaaa tgccaaaacc gattccgacg
    12421 aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa gccctgctca
    12481 aacaaagcga aggaatgctg cttcatgtca gcaattccct caaacagctt caggaagagc
    12541 gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc aaccttgctt
    12601 ccgtcaaccg caaacagcgc gaggcttggg acaagttcca aaaactcaat accgagctga
    12661 accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc gtatcgggga
    12721 actataaaaa cagccagccg aatgcggttg ccctgttcct gaaaaacgcc gaaccgggtc
    12781 agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc ctccaatcgg gaagttgtca
    12841 aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc aacaatgagc
    12901 ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa cagggtgtaa
    12961 ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc aaagatgccc
    13021 gaaaactgct ggaacagaaa gggaacgagc agcagctgaa caagctcttg agcaatttgg
    13081 agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa ttggctgaag
    13141 ccaggctggc ggcagccgaa aaagccagaa aagaagcggc gcagcagaag gctgaagcac
    13201 gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa catccaagcg ccttcggtta
    13261 tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg acgtttgaaa aaaccggttg
    13321 acggtgtgcc gaccggactt ttcgggcaga accggagcgg cggcgatgtt tggaaaggcg
    13381 tgttctattc cactgcaccg gcaacggttg aaagcattgc gccgggaacg gtaagctatg
    13441 cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga tcacggcgag aactacatca
    13501 gcatctatgc cggtttgagc gaaatttccg tcggcaaggg ttatatggtc gcggcaggaa
    13561 gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga ggggctttac ctgcaaatac
    13621 gttatcaagg tcaggtattg aacccttcga gctggatacg ttgatcggat tgccgaaacg
    13681 gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc cgtgaagttt gccgtacctg
    13741 tattttccgg tttttggaaa tcaagagaga aagttaaaga agaatgtcga aacctgtttt
    13801 taagaaaatc gcactttata ctttgggtgc aatcagcggc gtggccgtca gtctggcggt
    13861 gcagggtttt gccgccgaga aggaccggcg ggataacgaa gtcctgccgg tgcaatccat
    13921 ccgcacaatg gcggaggttt acggtcaaat caaggcaaac tactatcagg acaaacccga
    13981 tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc ggtttggatc cgcattccga
    14041 atatatggat aaaaaaggtt atgccgagat aaaggagtcc accagcggcg aatttggcgg
    14101 cttggggatg gaaatcgggc aggaagacgg atttgtcaaa gtggtttcgt cgattgagga
    14161 cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc attgtgaaaa tcgataatgt
    14221 ttcgacgcgg gggatgacag tcagcgaagc ggtgaagaaa atgcggggca agccgggtac
    14281 gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag ccgatagtcg tcaacctgac
    14341 ccgtgccatt attaaagtga aaagcgtccg ccatcacctg atcgaacccg attacggcta
    14401 tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc gtcaataccg ccgcaaaaga
    14461 gctggtaaag gaaaataaag gaaaaccgct caaggggctg gtgttggatt tgcgcgacga
    14521 ccccggcggg cttttgaacg gcgcggtcgg cgtgtcggcg gcgtttttgc cgcctgatgc
    14581 gctcgttgtc agtacgaaag ggcgggaagg tacggaaggc tttgcgttaa aggcgcgtcc
    14641 cgaagattat atttttaaag ccggcacgga tcctttggcg ggtattcctg ccgagttgaa
    14701 aaccataccg atgacggttc tgattaattc gggttcggct tccgcgtcgg agattgtcgc
    14761 aggtgcattg caggatcata aacgcgcggt tatcgtcggt acgcagagct tcggcaaagg
    14821 ttcggttcag actttgattc ctttgtccaa cggcagcgcg gtcaagctga caacggcact
    14881 gtattatacg ccgaacgacc gttctattca ggcgcagggg attgttcccg atgtcgaagt
    14941 aaaagataag gaacgcattt ttgaaagccg cgaggcggat ttggtcggac acatcggcaa
    15001 tcctttgggc ggcgaggatg tgaacagtga aacccttgcc gtgccgcttg aaaaagatgc
    15061 ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag gacgaggatt tgtcttcaag
    15121 gcggattccc aaccctgcca aagacgacca gttgcggaaa gctttggatt tagtcaagtc
    15181 gcccgagcag tggcagaagt ctttggggct ggcggcgaaa aagccggttt caaataaaga
    15241 taagaaagat aaaaaagata agaagtaggt tttgacgatg ccgtctgaaa ggatttcaga
    15301 cggcatatta tgtttgaaag gacaggttat gagcgatttt ttaccggaat ttgaaccgtg
    15361 cggcatttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
    15421 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta tacttgggta
    15481 gcgattgggt ttcagacggc atacattcag gatttcaccg ccttatgccg tctgaaagac
    15541 gtttccgacc catccctact acacgctttg tccgcaaacg catcacaggc aagattatgg
    15601 aagtcatccg ataccccgat tcccctttca aactccacca acccttcccg cccgcaggcg
    15661 accagcctac cgctattgcc ggcttgctcg aagggctttc agacggcctt gcctaccaaa
    15721 ccctgctcgg cgtaaccggt tcgggcaaaa cctacaccat ggcgaacgtc atcgcccaaa
    15781 gcggccgacc cgccatcatc atggcgcaca acaaaaccct tgccgcccag ctttatgccg
    15841 aaatgcgcga gtttttccct gaaaacgcgg tggaatattt cgtctcctac tacgactatt
    15901 accaacccga agcctatgtg cccagccgcg acctgttcat tgaaaaagac agcgcgatca
    15961 acgaacatat cgagcagatg cgcctttccg ccaccaaaaa cctgatgacg cgcgatgacg
    16021 tgattatcgt tgccactgtg tccgccattt acggtatcgg cgacccgact gagtatcaac
    16081 aaatggtgtt gtccgtcaaa gaaggcgaca ccatcgagca gcgcgacatc atcgccacgc
    16141 tcgtttccat gcagtacgaa cgcggcgatt tggatttcaa acgcggcagc ttccgcgtgc
    16201 gcggcgacgt gattgacgtg taccccgccg aaagctccga aaacgccttg cgcatcagcc
    16261 tgtttgacga cgaaatcgac cgcctcgata tgttcgaccc gctttcaggc agcctgcacc
    16321 aacgcgtcgg ccgctacacc gtcttcccgt ccagccacta cgttaccccg cgcgacaccg
    16381 tattgcgcgc ctgtgactcc atcaaagaag aattgcgcga acgcattgat ttctttacca
    16441 aagaaaaccg tcccgtcgaa caacaacgca tcgaacagcg cacccgcttc gacctcgaaa
    16501 tgctctacga aatgggcttc tgtaaaggaa tcgaaaacta ctcccgccac ttctccggca
    16561 aaaaagaagg cgaaccgccg cccacgctga tggactacct gccaagcaac gccatcatgt
    16621 tcatcgacga aagccacgtt accgtaaccc aaatcggcgg tatgtacaaa ggcgacgcat
    16681 cgcgcaagca aaacctcgtc gattacggct tccgcctgcc ttccgcccgc gacaaccgcc
    16741 cgctcaaatt ccacgaattt gaaaaagtca tgccgcaaac cgtcttcgtt tccgccaccc
    16801 ccgccaaata cgaagaagaa cacgccggac aagtggtcga acaagtcgtc cgccccacag
    16861 ggctggtcga ccctcaaatc atcatccgcc ccgtcgccac ccaagtcgac gatttaatga
    16921 gcgaaatcaa cgaccgcatc caaaaaggcg aacgcgtact cgttaccacc cttaccaaac
    16981 gcatggcgga gcaactcacc gactattaca gcgaactcgg catcaaagtg cgctacctgc
    17041 acagcgacat cgacaccgta gagcgcgttg aaattattag agatttacgg ctcggattgt
    17101 ttgacgtact cgtcggcatc aatctcttgc gcgaaggcct cgacatccca gaagtctccc
    17161 tcgtcgccat cctcgacgca gacaaagaag gcttcctgcg ctcccaccgc agcctgattc
    17221 aaaccatagg ccgcgctgcg cgcaacgtga acggcgtcgc gattctgtac gccgacaaaa
    17281 tcaccgactc tatgaaagcc gccatcgacg aaaccgaacg ccgccgtgaa aaacaaatta
    17341 aattcaacga agaacacggc atcgtgccgc aacagattaa aaaacaggtc aaagacatca
    17401 ttgacggcgt gtaccacgaa gaagacagcg gcaaaggccg tagacaaggc aaaaacaaag
    17461 ttaaagtcgg cgaaattcac aacgaagaag acgcgattaa agaaatcgcc aaactcgaaa
    17521 aagccatgca acaggcggct agggatttgc agtttgaaga agcggcggtg ttgagggata
    17581 ggattaggaa tattaaagag aatttgttgt ttggggcaga gtaaaggagc ttgatttttt
    17641 aattagcgaa gatacaataa cgtcatctat tatttttata ggaaagtaac tatggcagag
    17701 caactacagc tgattcctcg tgttgaaagc ggtgaaatta tcccacagag aatgagtgac
    17761 ggatatatca atgcgacagc attgtgtaaa tctgtaggta aatcttacag tgattataga
    17821 caattacagt ctacaaatca tttcttaaat gaattgaaag cctagacggg attatccgaa
    17881 caacaattga ttcaacagag aattggtgga gaaccttctt tacagggctc ttgggtacac
    17941 ccttatttag caattaattt agcacagtgg ttatcccctg catttgctgt aaaggtatct
    18001 acttgggttc atgaatggat gtcaggtaaa gtcaatgctt ctccttccca ttccgcgcat
    18061 atccaaagat acttgcaaaa tagaggaaaa attccatata cgcatttttc aatgcttaat
    18121 gaaattacgc taaatttaat tgctcctttg gaggaacaag gttatgtatt acctagtcag
    18181 ttagttccgg atatttcaga aggaagatta ttttgtagat ggctgaggga aaatagaaac
    18241 gttgatccga ataacttccc aacatataaa catgaattcc ttgatgctcg gagacccgtg
    18301 gaggccaaat tatatccaat agaatactat caagatttta aatggcactt taatgaagta
    18361 tggttaaaag aaaaagctaa acaatatttt tcagaacgtg atgcagcaat tttgccttat
    18421 ttaaccccgt taatgttacc agcaagcgag tcgtagcaag ccgtagcctg cattaccaac
    18481 caccgcgtgc gtagctacgc catacaccct acctgcgggc gggacaaact ttaaggggct
    18541 gtactagatt agccctaaat cccataccaa tcccgcagga ttttaagctg ttgagacggt
    18601 gtgccgaagt taaatcgaaa ttagcattct ttcaagaaca gtggaaaaga tttgcaatcg
    18661 attccgttgt attttcgcaa gacgcgtttt gcctgattcc aaaaattctc aatgccgtta
    18721 atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg gataaaaccg
    18781 ctcacgtcca acttgtcg
//
LOCUS       DAWFKN010000035        18421 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFKN010000035 DAWFKN010000000
VERSION     DAWFKN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.035"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..163
                     /locus_tag="WGR46_001164"
     CDS             <1..163
                     /locus_tag="WGR46_001164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856109.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            200..412
                     /locus_tag="WGR46_001165"
     CDS             200..412
                     /locus_tag="WGR46_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0856110.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WGR46_001166"
     CDS             560..2143
                     /locus_tag="WGR46_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0856111.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="WGR46_001167"
     CDS             2227..3324
                     /locus_tag="WGR46_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0856112.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFLWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="WGR46_001168"
     CDS             3438..4553
                     /locus_tag="WGR46_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0856113.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="WGR46_001169"
     CDS             4678..5133
                     /locus_tag="WGR46_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0856114.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="WGR46_001170"
     CDS             5204..5680
                     /locus_tag="WGR46_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0856115.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5668..6594
                     /locus_tag="WGR46_001171"
     CDS             5668..6594
                     /locus_tag="WGR46_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0856116.1"
                     /translation="MSEMKKNKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6659..7216
                     /locus_tag="WGR46_001172"
     CDS             6659..7216
                     /locus_tag="WGR46_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0856117.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            7580..9271
                     /locus_tag="WGR46_001173"
     CDS             7580..9271
                     /locus_tag="WGR46_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0856118.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            9284..12223
                     /locus_tag="WGR46_001174"
     CDS             9284..12223
                     /locus_tag="WGR46_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0856119.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYSDNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(12317..13276)
                     /gene="ttcA"
                     /locus_tag="WGR46_001175"
     CDS             complement(12317..13276)
                     /gene="ttcA"
                     /locus_tag="WGR46_001175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0856120.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(13374..14237)
                     /locus_tag="WGR46_001176"
     CDS             complement(13374..14237)
                     /locus_tag="WGR46_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0856121.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATIKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            complement(14272..14634)
                     /locus_tag="WGR46_001177"
     CDS             complement(14272..14634)
                     /locus_tag="WGR46_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0856122.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKKDAATA"
     gene            14709..15188
                     /gene="ybaK"
                     /locus_tag="WGR46_001178"
     CDS             14709..15188
                     /gene="ybaK"
                     /locus_tag="WGR46_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0856123.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPRDLNILNPKTIQAAV"
     gene            15211..15933
                     /locus_tag="WGR46_001179"
     CDS             15211..15933
                     /locus_tag="WGR46_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0856124.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            15935..16810
                     /locus_tag="WGR46_001180"
     CDS             15935..16810
                     /locus_tag="WGR46_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0856125.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFLLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(16882..18279)
                     /gene="aspA"
                     /locus_tag="WGR46_001181"
     CDS             complement(16882..18279)
                     /gene="aspA"
                     /locus_tag="WGR46_001181"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0856126.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         4770 a         4433 c         4874 g         4344 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agacggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atctgcttga
     1141 agaacccttg gaagcgcgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaaaggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgttaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcaggggat gattatattg ccaaagaatt ttatagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctag aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 caccacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac actgcgaaaa gcggtacacg
     2221 acacatatga accatacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
     2581 gagggattgt ttgcctgttt gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt acattcctcc gatttgagtg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgc
     2761 cctttgcttt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cttgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttccgacggc agccacacgc gccagcgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acaggtttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt tttgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtcttcaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatac cttgcccgct tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgtgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga cagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggccgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accggaatct gtaaaaaatg tcagaaatga aaaaaaacaa agtccacctg
     5701 atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat ggcgcaaaaa
     5761 gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgagatcgg tattgacggc
     5821 gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg tttgtgttgc
     5881 accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc caagcccgac
     5941 cgcctgatga ttgaagcaag cggactggct cacgcagcca gcgtcatcga cgaactcaaa
     6001 gccaaaccgc tggacagcct tttggaaatc ggcgccgtct ttaccgtcgt caatccgcgc
     6061 cagttcatca accccgatta cgcgcaacag gcgttgtata aagaccaaat cggcatctgc
     6121 gacgtattgg tcgccagcaa aaccgattta tgcaccaccg aacagcttgc cgaatttcac
     6181 gacaaagcgg caaaactgtt cccgcccaaa gccaaagtgg tcgaagtcca aaacgcacaa
     6241 ctcgacatcc ggtggctcga tattcccgtc atcgaaaaat cacgctaccg cctcaaagcc
     6301 ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc cggacgcgat
     6361 ttcgacggcg aaaagctgac caactttttc aacgacctgc ccaaaatgac cgaaggactc
     6421 gtccgcgcca aaggcgtgtt tcaagtattg ggaacgtggg tgtggctcaa ctgggtagac
     6481 ggacagtggg gcgcgaacca agtctcatgg cggcgcgatt cgcgttttga attgattgcc
     6541 aaatcgtttg atgcggattt aatcgaacaa aagctcaaag acgcattgga atagcgttca
     6601 cgcgccttca gacggcatat gaaatgaaaa tgccgtctga aggtctgagg aggacgggat
     6661 gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttccc tgccgtctat
     6721 ggcggcaacc catgttttga tggaaaccga tatgggcaat atccgtttgg ttttggacga
     6781 atccaaagca cccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag gcttttacga
     6841 caatacgatt tttcaccgcg tcatcggcgg cttcgttatc caaggcggcg gattgaccga
     6901 ggacttggca caaaaggcaa gcgataaggc cgttgccaac gaatccggca acggcttgaa
     6961 aaacactgtc ggcaccatcg ccatggcgcg gacgacagac cccgattccg ccaccagcca
     7021 attctttatc aatctggcgg acaatgattc actcaactac aaaaacggac aatacggcta
     7081 taccgttttc ggcagggtcg aaagcggaat ggacaccgtt tccaaaatcg cccgcgtcaa
     7141 aaccgccacg cgcggctttt atcaaaacgt acccgtacag cccgtcaaaa tccgtcgcgt
     7201 tgttgtcggg cagtaacacg cagacagatg ttcagacggc gtcgcccgtt tcacaaaaaa
     7261 cgccgtctga agtgaaaaaa tattttaaaa cagatagttg atattgacaa attcaaaccg
     7321 aggattttaa aatgctgcca acccaaccca acccaaccca acccaaccca acccaaccca
     7381 agcccctgat ctaatcgaca cagccggggt gaacaccgag gcaatctaca cttcagacgg
     7441 cattaccgcc aactctaccc aactcgaaca gctcaaaaaa ctgtttcccg cctgttttga
     7501 cgcagacgga aatttcctta tcgacagatt acaagccgaa attgcgccac agaccgacat
     7561 cggacgcgaa ttttacgaaa tgaactggct gggcaaatca tatgctcgcc tgcttcgcaa
     7621 cctgccgccc gaaaccctga taagtgaaga caagacgcac aacgccaaac ccgaaaatgc
     7681 aggcagccaa aacctactga ttcgtggcga caatctagaa gtgttgaaac acttaaaaaa
     7741 cgcctacaca aacagcgtga agatgattta catcgacccg ccctacaaca ccggatcaga
     7801 cggctttgtc tatcaggacg accgcaaatt cacaccagcc gaacttgcct gcttagccaa
     7861 tattgacgaa gacgaagccg cgcggatttt agatttcacc gacaaaggct caaactcgca
     7921 cagtgcatgg ttgaccttta tgtatccgcg cctgtatatc gcccgcgaac tgttaaagga
     7981 cgacggcgtg atttttatct cgattgacga taacgaagcg gcgcagttga agttgttgtg
     8041 tgatgaagtg tttggggaag ggaattttat taaagattta attgttaata catcagaagg
     8101 tggtggtaat gcaaaatatg ttgtaaatgg gcatgaaact gttttagttt atgcgaaaga
     8161 tattactcat tttgacaatc taaaaagacc aaaagatata cgcggcaaaa aaattgtaat
     8221 caatgatgaa ttatattgga tacaggaaga tgccgttcgc gaacagtttg gcaaatatgg
     8281 gaacttgcat tacgaagata ttattgaaat aaaaggaatt gagtttaaaa acaaaattga
     8341 taaagatatt gaaaataatg aatatatctt aattccgaag tcttatggga aaacaattat
     8401 tggaaaactt agaaaagtat ccgatgattt ttccaaattt catagcatct taaatattgg
     8461 aaatataagt aaaaatctta ctaaagatgg tattcgtgaa ttagaagagt tgtttgaaac
     8521 tggaaaaggt tattctcctt ttgaaacacc aaaaccatta gatttattga aaagattagt
     8581 tctttctgca actttcaagg gaaatcaaac tgacctaatc ctagacttct tcgcaggcag
     8641 cggcacaacc gcccacgccg tgatgcagct taacgccgaa ggacaaaacg gtaaccgccg
     8701 ctatatctgt gtacagcttc ccgaaaaaac cgctgaaaaa tccgaagccc gtaaagcagg
     8761 ctacccgacc atcttcgaca tcaccaaagc ccgcatagaa aaagccgccg ccaaaatccg
     8821 cgtcgaacat cccgattaca cgggcgattc gggcttcaaa atctttcaaa cggcagacaa
     8881 tttcaggcag catccggaca aggatttttc gcccgaacaa ccggatttgc cgcttaacga
     8941 tgaattaagc gaagaacagc tgcaaacgct tctgaccacc tggacgctgt atgacggggc
     9001 ggcactgacc acgccggttg agcctgtgcg gttaggggct tacacggcgt atctgtgcga
     9061 aaaacggctg tatctgatga atgccggttt tacttccgcc gatttgttgg cgtttatccg
     9121 caagctggac gacgatgcgg atttcaatcc caaccgcgtg attgtattcg gcagcaatat
     9181 ggcaagcgcc atgcagcacg aacttgacca ggcggttcgc ggttatgcca ataaaaaaga
     9241 gattgagttg aatgtggtta tcagggtttg acggaggcaa ttcatgagcg gttttaatta
     9301 cgagaaaaac cagccgcacc aaatgcgggc ggtttcggcg gttttgggcg tgtttgacgg
     9361 ggcaacgccc aaatatcggg cggcagacga aaatcccgaa cttttgtttg ctgcaaaaca
     9421 atactcagac aatatcctga aagtgcaaag ccaaaacggt atagacggcc gattccccga
     9481 ccgttcggac gaccaaaata tccttgatat ttccatggaa acgggcacgg gcaaaaccta
     9541 tacctacaca caaaccatgt tcgagctgca ccgttggctg ggcgtgttca aatttatcgt
     9601 ggtcgtgccg actttgtcca ttaaggcggg aacacagcag tttttgcaaa gcaaggcttt
     9661 ggcagagcat tttgaacagg atttcggcgg cgattatgaa ggcgtgcgcc tgaaaaccta
     9721 tgtggtggaa agcgcgaaaa agaataaggg caaaaagtcc aatgcgccca taacgattga
     9781 gcaatttgtc aaagcggaaa acaaaaagga aatccatgtg ctgctgatta acgcgggcat
     9841 ggttaattcg tcgtccatga acgatacggg cgacaaggca ttgaaagatt tgtttgacaa
     9901 tcccgttgat gcattggccg ccgtgcgccc gtttgtgatt gtggacgaac cgcataaatt
     9961 cccgacccgg gagagcgcga aaacgtgggg caatatcaaa cgcttaaaac cgcaatatat
    10021 tttgcgctac ggtgcgacat ttaacgatga atattacaac ttgctttacc gtttgacggc
    10081 agtagacgcg tttaacgacg ggctggtcaa aggcgtgcgc gtgtttcagg aagaaatgca
    10141 gggcggcatg gatgcggcgg taaaactggt gtcgtcgggc ggcaaagaag cgaaatttga
    10201 attaaacgaa aaggacaaaa agcagacgtt caaactggca aaaggcgaag atttggcgca
    10261 aatccatccg gctatttcgg atttgaaaat cgacaaaatg aataaaaccg tggtggtgtt
    10321 aagcaacggc ttggagttga aaacgggtgc cgtcatcaac ccttattcct attcgcaaac
    10381 ggtgcaggat gcgatgatgc agcgggcggt tgccgaacat ttcaagctgg aacgcgcgct
    10441 tttggcagaa cgcgcgccac agcccaaaat caagccgctg acgctgtttt ttattgatga
    10501 tattgcgggc taccgcagcg gcaacgagct ttcaggcagc ctgaaagata aatttgaaag
    10561 ctggattcac gcggaagcgg cacgccgcct gaaaacggaa agcgacccgt tttaccgcga
    10621 ttacctgcaa aagacgttgg acgatgtatc cgcctgccac ggcggttatt tttccaaaga
    10681 caatacagac agcgacgata gaatcgagca ggaaatcaat gaaatcctgc acgataagga
    10741 aaaactgttg tctttggaca acccgcgccg ctttattttt tccaaatgga cgctgcgcga
    10801 aggctgggac aatcccaacg ttttccagat ttgcaaactg cgttccagcg gcagcacgac
    10861 ttccaagctg caagaagtcg gacgcggcct gcgcctgccg gtaaacgagc tgatggcgcg
    10921 ggtgcgcgat gtgccgtaca aactgaatta ttttgtcgat agcagcgaaa aagactttgt
    10981 gaagcagctt gtcggcgaaa tcaacgataa ttcttttcag gaagaaatct ccaaaaagtt
    11041 taccgaagag ctgaaacaaa aaatattgca aaaatacccc gatatcaaac cgctggtatt
    11101 ggtaaaccaa ctgttttcag acggcatcat tgacgacaat gaaaacttta ccaaagacgg
    11161 ctatgacaaa ttaaaagccg cctatcccga agccttcccc aaaggtttgg acaaaggcaa
    11221 agtcagcaac gccaaagacg agggcaaaga caccatcatc atgcgcgaag gcaaatatga
    11281 agaactcaag gccttgtggg agctgattca ccataaagcc gttttgcagt acaaaatcaa
    11341 ggatgaagcc gaatttgtcg atttatttac cgcctatttg cgtgaaaacg ccgccaaatt
    11401 cccgcaggca ggcatacgca cggcggtaaa cgaagcttat atcaacaacg ggcttatgct
    11461 ttcccgccgc atagacagta ttgaagatga agattttatc cgtttcaaca caatgactta
    11521 ccgagagttt ctggaaaaac tggcacaaac ggcaaaaatc cgtatgcaga ctttgcatca
    11581 ggcgttttac cgcatccgca acgaactgaa cattggcgat tttttgaata tgcaaaccat
    11641 cgcccaaatc aaaaacggct tcaaccggtt tttgcttcat cattccttcc ataaattcga
    11701 actggattac cggcttgtcg gcagcaaaat ccatccgacc aaatttacca ataaagacgg
    11761 caaaccgcgc gcggtgaaaa aagcagattt gggcagattt gaagatacgg agcaccggcc
    11821 tgccgccggc tatctcttcg acgagatttt ctacgattcg gatatagaac atgaaaatgt
    11881 cgccaacaac caaattgaag gcgtaaccgt atttaccaaa ataccgagaa actccatcaa
    11941 aatccctgtt gccggcggcg gcacgtattc gcccgatttt gcctatatcg tgaaaaccaa
    12001 aagcggcgag atcctgaact ttgtgattga agccaaaggg actgacgggg cggaagattt
    12061 gcgaaaaagc gaagagcgga aaatcaaaca tgccgaaaag ctgtttgccg agatttccaa
    12121 agaaatcaag gtggtgttca aaacgcagtt tgacggcgag aggatagccg aactgatcgg
    12181 gcaaaatatg cccgcaggcg ggcattctga aaacggacac tgagaaacaa atgtttggaa
    12241 gccgtctgct tcgggatttt aaaatgccgt ctgaaacgcc aaacgggctt cagacggcat
    12301 ttttgacggc ggggggttac gcgccgcagg ttttcggctt gttcgccaga atgttgatga
    12361 ctttgcgttc ggctttttgc ggcgcgattt tgatttcgct ttcgtcttct tcgctgccgt
    12421 ctgaaaaacg ttcgggcatt ttttcgctgt caaacgccaa atcgccgccg tgtttcaggg
    12481 tttgaccgcg ttccaatccg gcaaagtcga agagttcggt atcggcaagg tgggaaggga
    12541 cgacgttttg cagggcggag aacatcgatt cgatgcggcc gggaaagcgt ttgtcccaat
    12601 cgcgcagcat atcgccgatg acttggcgtt gcaggttggg ttgcgagccg cagaggttgc
    12661 acgggatgat ggggaattgt tttaattcgg cgtatttgat taagtctttt tcttttacat
    12721 acgccagcgg acggatgacg atgtgttcgc cgttgtcgct caccagcttg ggcggcatgg
    12781 ctttgagttt gccgccgtaa aacatattta aaaacaaggt ggcgaggatg tcgtcgcggt
    12841 ggtgtcccaa ggcgattttg gtgcagccca attcttttgc agtgcggtag aggatgccgc
    12901 ggcgcaggcg gctgcacagc gaacaagtcg ttttgccttc gtctaatacg cgtttgacgg
    12961 tggagtaggt gtcttcttca acaattttgt agggaacgcc gatgctttcg agataggtcg
    13021 gtaatacttc ttcggggaag cccggctgct tttggtcgag attgacggca accagttgga
    13081 aatcaatcgg cgcgctggct tggagctggc gcaggatgtc caagagggca tagctgtctt
    13141 ttccgccgga gaggcagacc atgattttgt cgcccggctc gatcatgttg aaatcgttaa
    13201 tcgcgtcgcc gacggcgtgg cgcaggcgtt tgctgagttt gttgttttcc agttcttgtt
    13261 tggttttttt ggacatggcg gtggtttgaa aaattgggga tgcggcattg taaccgaaaa
    13321 caggggcggt tttaaaatgc cgtctgaaaa aggttcagac ggcattagag ggattattct
    13381 gcattttcgg ttttaaagaa gggatgaacc gctttgaaga tactgccgtt tggggcggtc
    13441 agttcttttt gcgcggcgga gaatttaatc acggcaaggg cggtaaagtt ttccgaatct
    13501 tgaacgctgt cgagtacgat gccggtctct tcgccgtctg ccgtcagcag ggttcctgct
    13561 tcgacggccg aatttcccga caataccgcc aagccgcgtt tgacctgacc gcgatactgg
    13621 gcacgggcaa tgatttcctg tcccggatag cagccttttt tgaagtgtac gccgccgatg
    13681 atgtgctggt tgagcatttg ggcgacggcg gtttctttgg tagccgcgca tatccacggg
    13741 taaccgctgc ggatttcgtg cagccgccac gcgttttcgg cggcggcatc ataaggcggc
    13801 aaggcgtttt tgggggcgat gtgcaaaatg ccccgatggg gcaggacgac ggtgcagatg
    13861 ccgtctgaaa cgcattcggc ggtaaagacg agactgggtt cttgcgcggc aagcggttcg
    13921 gcggatgctt ccaattccgc gtcgacggcg taatcttcaa ggatttcaaa aacgacttta
    13981 gcgcgcagga caaacatccg caggcgtttg atcgttgctt caagcaagtc ttgcgccata
    14041 atcagcagca aatcgccgcc tcggttgacg acaatcatat tggcgatgac gcggcctttg
    14101 ggcgtgttgt aagtcgcata acacgcctgc ccggtctgaa gattattgat gtcgttggaa
    14161 agctgtccgt gcaggaaggt ttggcggtct tcgccgctga cgcgtaccac gccgaaaaag
    14221 ggcagtaagg ttttcatcat ttgcgtactc tgaaatataa aggaaatctg tttatgcggt
    14281 tgccgcatct ttcttcacgg cggttatttt gatttcgacg caccactccg gacgggcgag
    14341 ccgagcttcc acgcaggcgc gggcgggcgt tctgccggcg gcaacccaag cgtcccacac
    14401 gccgttcatt tccgcatagt cgcccatatc gcgcagatag atgaccgcat ccaaaacgtg
    14461 cgctttgtcc gagccgcatt ccgccagcca gcggtcgatt tgggcaagca cgtcggcagt
    14521 ctgttcggca gccgtttcac cgttttcggg aaccatgccg gagaggaaaa tcaagccgtt
    14581 tgcgccgacg gcttcggaat agcggggcgt tgtgccgaaa tatcggatat ccatatcggt
    14641 ttccttcgat aaaggggata tatggtaaca ttgcgcttga ccgatttcca tgttttgcat
    14701 gacgaaaaat gagtaaacat acttatccga taacacctgc cgtgcgcgtt ttgcgtgaaa
    14761 acggcatcga atttgaacct tttacctatg cctatgagga acacggcggc acggcgcagt
    14821 ttgcccgact attcggcaaa gacgaacact tggtcattaa aaccattgtt ttgcaagatg
    14881 aaaacggtaa ggggctgatt gtcttgatgc acggcgacaa gcagatttca acccgcaatc
    14941 tggcgcggca tttgggtgcg aaacacattg aacccgccac gcccgtacag gcaaacaagt
    15001 ggacgggcta tctggtcggc ggcacaacgc cgttcggcat ccggacaaag ttggatattt
    15061 acgtcgaaca gtcggtgatg gatttggaaa ccatctatat caacggcgga aaacgcgggt
    15121 tcattatcgg catccgtccc agagatttaa atattttgaa cccgaaaaca atacaggcgg
    15181 cggtttgacg ggaaagtata aaggaacaat atggacaaag atttgtatgc cgtattgggc
    15241 gtgtcgccgc aggcgggagc ggacgaaatc aaacgcgcct accgcaagct ggcgatgaaa
    15301 tatcatcccg accgcaatcc gggcaatccg aaggcggaag aaaagttcaa agaaatccaa
    15361 cgggcttacg atacgctttc cgacctgtcg aaacggatgc aatacgacgc gtccttcaga
    15421 cggcatgagg aacgcgggcg gcaggaagag gcattccgcc gcgaacaggc gcgcagggag
    15481 cagttttacc gcgaacagat gcgccgcgaa caggcgttca gacaggcgtt tgaacggcag
    15541 gcatcacgtt cgtgccatac ttacgaaccg tccggcggcg gaagcgggcg caactatgtc
    15601 ctcgccgcct acatcctgtt cggtttgggt gcaatcatgc tgttcatgcc catagtcggc
    15661 gtgattttcg cctatatgcc catagtcggc gtgattctcg cctatatgaa acggaacagt
    15721 ttggacagca ttgtctatgc cgcacatacc gaatacctga ttaaaacctt ttggcgcaca
    15781 ttttggcttt atattttggg tgcgctgact gcccttttgg gtatcggcgt gctgattatt
    15841 attgcaacga acgtctggta tttctaccgc atcatcgccg gctttatccg cttcaacggc
    15901 ggcagggcgg ttgcacccga gaaatggata tagtatggct tacctgttaa tcagcatcgt
    15961 gttcagcgtg tcggtttcca ttttgctgaa aatggcaagg aagaaaaaaa tcgacatcgc
    16021 gcaggcggtc gccgtcaatt atgtggtcgc ggtcatactg accctgctgg tattgaagcc
    16081 ggatgtcggc aatatcggcg catttttgcc gacatggtgg ctgtttgccg cattgggcgt
    16141 gctgctgccg tccgtatttg tcattatggg caaatccgta gaatccgccg gtatcgtcaa
    16201 atccgacgcg gcgcagcgtt tgtcgctgtt tttgccgatt gttgccgcct tgacgctgtt
    16261 tggcgaaaaa ctcagcgaag gcagactaat cgggctgtgc ctcgcatttg ccgcactgtt
    16321 ctgcctgctt tggaaacaca gcggtggcaa aaaatcagga agcgcgtggc ggcaggcggt
    16381 attgctgctg ggcgtgtggg caggttacgg cattatcgat atcctgttca aacagcttgc
    16441 caaaagcgga acggcatttg cgggcaacct gctggttgca tttttgctgg cgggtgtgct
    16501 gatgtttgcc tgcctgtttg ccaaatcggt cagatggcgc gttgagagtg tggtcggcgg
    16561 catactcttg ggcggtttga acttcatgaa catcgtaacc tacatcaccg cgcaccaaat
    16621 gatgaaggac aatcccacgc tggtttttgc cggtatgaat atcggcgtga ttgttttggg
    16681 gacgctttcg ggcgcactgt tttttaagga aaaaatcaac acaatcaata cggcgggaat
    16741 cgtgttggca ctgtgttcca tcgcctgcct gttttattgg gcggaggtca aggcactgtt
    16801 cggcatataa tccggctgat tgatataaca aaatgccgtc tgaagcagca tccctgcttc
    16861 agacggcatt tgtctgcaac gttacagatg ggggttcatc aggttttcag gagagaggat
    16921 gcggttgagt tcttcttcgc tcaacagccc gcgttccaag acaacctcgc gcacgccttt
    16981 gccggtttgg gcgcagattt tgccgaccaa atcgccgttg tggtgtccga tatacggatt
    17041 cagataagtc accaaaccga tggagttgaa aacgtaacgt tcgcagattt cgcggttgac
    17101 cgtaatgcct ttgacgcatt tgtcggacag gttgactgcg gcattgccca agagggaaat
    17161 ggtttcaaac atacattggg cgatgaccgg ctccatcacg tttaattgca gttgcccggc
    17221 ttcggcggcg aaggtaatcg tcgtgtcgtt gccgatgact ttgaagcaga cttggttgac
    17281 cacttcggga atcacgggat tgactttggc aggcataatg gaagaaccgg cctgtaattc
    17341 gggcaggttg atttctttca agccggcacg cgggccggaa gagagcaggc gcaagtcgtt
    17401 gcagattttg gagagtttga ccgccgtgcg cttcaatgcg ccgtgtacca tcacatatgc
    17461 gccgcagtcg gaggtcgcct caatcaggtt ttcggtcagt ttgcaaggca agccgctgac
    17521 ttcggagagt tttttgacca ccagttcggc gtagcctttg ggcgtgttca cgcccgtgcc
    17581 gattgccgtt gcgcccaaat tgacttccaa cagcagttgg cgggtgcggt cgaggttgag
    17641 gatttcttct tccaacaaca cttggaaaga ttggaattct tggccggcag tcatcggcac
    17701 ggcatcttga agctgggtgc ggcccatttt caaaacgtct ttaaattctt cggctttggc
    17761 ggcaaaggcg tttttcagga cagtcagttt gtcgagcaat tcgccgatac tgtaatacac
    17821 ggcaaggcgg aagccggtag ggtaggcatc gttggtggat tggctggcgt tgacgtgatc
    17881 catcggattg acgatgtcgt agcggccttt ttcgtatccc aaggcttcca atgcgaggtt
    17941 ggcgatgact tcgttggtgt tcatattgac cgaagtaccc gcaccgccct gatacacatc
    18001 ggacgggaat tggtcgaggc agcggttgtt cagcagaact tcgtcgcaag ccttttcaat
    18061 ggcggcggcg atttcgggct tgaccgcgcc caacttaccg tttgcctgtg ccgtcgcctt
    18121 tttcaccatc accatactgc ggacaaactg cggcacgtca gaaatttttt gtgtggagat
    18181 tttaaagttt tcaatggcgc gcagagtgtg gatgccccaa tacacttcgg cgggaatctc
    18241 gcggtcgccc aataaatcgt gttcgatacg gacagtcatg tttttacctt tgtaagtcgg
    18301 ataattaata ttgaaaaaat gcgccatcgg aaagatgccg ccgcaggacg aacactatac
    18361 cggccggatg aaattgtcca tatcgtatgc cgtctgaaaa cgggaaacgt tgttttcggg
    18421 t
//
LOCUS       DAWFKN010000036        17143 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFKN010000036 DAWFKN010000000
VERSION     DAWFKN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.036"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(425..760)
                     /locus_tag="WGR46_001182"
     CDS             complement(425..760)
                     /locus_tag="WGR46_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0856127.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(841..2970)
                     /gene="dnaX"
                     /locus_tag="WGR46_001183"
     CDS             complement(841..2970)
                     /gene="dnaX"
                     /locus_tag="WGR46_001183"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0856128.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALRNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSVQTAEKEAAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1742..1781)
                     /gene="dnaX"
                     /locus_tag="WGR46_001183"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(3065..5041)
                     /gene="mutL"
                     /locus_tag="WGR46_001184"
     CDS             complement(3065..5041)
                     /gene="mutL"
                     /locus_tag="WGR46_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0856129.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQKDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHEITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNVLLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5185..5370
                     /locus_tag="WGR46_001185"
     CDS             5185..5370
                     /locus_tag="WGR46_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0856130.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5381..6049
                     /locus_tag="WGR46_001186"
     CDS             5381..6049
                     /locus_tag="WGR46_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0856131.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            6049..6765
                     /locus_tag="WGR46_001187"
     CDS             6049..6765
                     /locus_tag="WGR46_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0856132.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWLSL
                     IQTYPGSPAAKRAAAAIRKR"
     gene            6813..7298
                     /gene="purE"
                     /locus_tag="WGR46_001188"
     CDS             6813..7298
                     /gene="purE"
                     /locus_tag="WGR46_001188"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0856133.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(7481..8935)
                     /locus_tag="WGR46_001189"
     CDS             complement(7481..8935)
                     /locus_tag="WGR46_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0856134.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8932..9633)
                     /locus_tag="WGR46_001190"
     CDS             complement(8932..9633)
                     /locus_tag="WGR46_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0856135.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9630..10409)
                     /locus_tag="WGR46_001191"
     CDS             complement(9630..10409)
                     /locus_tag="WGR46_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0856136.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10694..12100
                     /locus_tag="WGR46_001192"
     CDS             10694..12100
                     /locus_tag="WGR46_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0856137.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            12212..13738
                     /locus_tag="WGR46_001193"
     CDS             12212..13738
                     /locus_tag="WGR46_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0856138.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            13934..14467
                     /locus_tag="WGR46_001194"
     CDS             13934..14467
                     /locus_tag="WGR46_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0856139.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14510..15811)
                     /gene="aroA"
                     /locus_tag="WGR46_001195"
     CDS             complement(14510..15811)
                     /gene="aroA"
                     /locus_tag="WGR46_001195"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0856140.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            16203..16679
                     /gene="greA"
                     /locus_tag="WGR46_001196"
     CDS             16203..16679
                     /gene="greA"
                     /locus_tag="WGR46_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0856141.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3636 a         4628 c         4768 g         4111 t
ORIGIN      
        1 gtcattcccg cgaaggcggg aatccagacc cctcggcacg gaaatttatc gagtaaaacg
       61 gtttctcaga ttctacgttc tagattcccg cgttcgcggg aatgacgggg tgtaagtttc
      121 tgctccgacg gggttggatt cccgctttca cgggaatgac gaagtttcag acggcattta
      181 agcggtacgg atgtgtaaat aatggtaggg tgggcttcag cctgccgatt cccgctattc
      241 ttgccgtttt tgcgttctta tcattctcac tgtttttacc gttcacgccg aatcctgcaa
      301 actgatgccg tcattcccgc gaaggcggga atccagaccc gtcggcacgg aaatttatcg
      361 agtaaaacgg tttcttagat tctacgttct agattcccgc ctgcgtggga atgacggtcg
      421 gggatcagcg gaagaagtcg cccactccgg gggggagtcc ttgggcgaat gcgcccattg
      481 ttttgtttgc ggtttcttcg gctttgccac gggcggattt gagggcggcg aggatgaggt
      541 cttcaagcat ttctttgtcg tcggcggctt cttgaatcaa atcggggctg atgtcgattt
      601 tgcgtacttc gtgcgcgcag gtcattgtga ttttgaccaa gccgttgcct gcttcgcctt
      661 cgatttcggt ttcggcaagt ttggcttgcg cttttttcat attttcctgc atttgctgcg
      721 cctgtttcat caggccgcct aatccggctt ttccgaacat actgaatact cctgtttgtg
      781 tttaaatgaa tgagggaaac ggcaatgccg tccgaagggt tcgggcggca ttatatctgt
      841 ttatggccgg tttgccgcca attccagtga ttcgggctgc cattgcgcgc cgaatgcttg
      901 gaggattttt tgtgcggcgg ggtcggcttc gagcaatgct tgtgcttttt gcctgtcctc
      961 ggcttggacg cgcttgtcct gcatcgcggg ggtttctctg ccggattcgt cctgccagtc
     1021 ttgggtttgc agggtgagtt gcagcccgta ggcttgggca agggtgtcgc ggattttgtc
     1081 gagacgtttt ttgtcggcgg tggcgcgtgc ttcggcggtc attgccaaaa ccatcaggcc
     1141 ggtgtcggga tggtattccg tccacgcgga gtgttgcgcc ggcatttgcg ccgcgccgag
     1201 tttgcgggcg aaatgccgga cgatggctgc ccagttttcg gtggaaaact cgggcggtgg
     1261 ggcggacggt gtgttgtttc cgccgatgcc gtctgcttct gcttcttcgt ccgcgcctcc
     1321 gcctgcggta tcggcagggg cggcgtgttc ccaatcgggc ggcgggattt ccgcgttgtc
     1381 ttctacgggg cagtcatcat ccggaaagct gtaaccgttg aaaggttttg caggagcttc
     1441 gtgcgcaaat gcttccgttt caagggcttc attattcggt gttgtctgaa tggggttttc
     1501 agacggcact tcggacaagg gggcattggt ttcgttgtcg gctgccttgt tcttggaaac
     1561 ttggttttcg ggcggcgttt cggcttcgga tgccgtctga atgctttttg ccgacgtttg
     1621 cgccgtgccg gctgcggctt gggcttcgtc tggcgcgtct tcccaaggcg gaacatcgtt
     1681 gttttcttga ttggaaacgg gttcggcggt tttgccttca gacggcattg ccgctgcgga
     1741 tgccgtctga acggatgttt gggcggtttc cgcttcaggg cgcggttggg gcttttttgc
     1801 ggcggcttcc ttttcggcgg tttgtaccga tggggattga agttcggtat tttcaatcac
     1861 ggcgtttgca tcacacgaag ctgccgccaa gggcgcaaac gccagcatac gcagcagggt
     1921 catcataaag ccggcgtatt cgtcgggggc gaggcccaag tcgcgtttgc cgtggacggc
     1981 gatttggtag taaagctgga tttgttcgcc acctatggtt tgggcgaggc ggtgcaaaat
     2041 atcggaatcg gggtcgtcgt gcgccaaggc attcggcact gcctgtatca gggcgagttg
     2101 ttgcagcagt atggcgagtt cgcccaaggc gttgtcaaag ccgacggcac acgccgccat
     2161 ttcccgcgct ttggcggtca gggctgcgcc gtcttggttg atgatgcctg tcagcagttc
     2221 gtaaaggtat tgtttgtcaa ccgcgccgat catttggcgg acatcgtttt cggcaacttt
     2281 gcccgaaccc agggcgatgg cttggtcgag caggcttaag gcatcgcgca tcgatccggc
     2341 ggcggcgcgt cccaaaagtt gcagggcggc gggttcgtag gcgatttttt cgctgtcgag
     2401 gacgtgggcg aggtggtcgg caacctgctg cgcggtcata ttgcgcaaga cgaattgcag
     2461 gcagcggctt aatacggtaa cggggacttt gtgcggatcg gtcgtggcaa ggatgaattt
     2521 gacgtgttcg ggcggctctt ccagcgtttt gagcatagcg ttgaacgcgc ttttggaaag
     2581 catatgcact tcgtcgatga tatagacttt gtatttgccg gcagtcggcg cgtattgggc
     2641 gttttccaag acttcgcgga tgttgtcgat gcctgtgttg gaggcggcgt cgatttcgag
     2701 caggtcgaca tagcgtccgg catcgatttg ggtgcagctt tggcacacgc cgcaaggttc
     2761 gccgtgttgc gcgttttcgc agttgaggct tttggcaagg atgcgggcga tggtggtttt
     2821 acctacgccg cgcgtaccgg tcagcaggta ggcgtggtgc agcctgcctt cgtccagcgc
     2881 gttgcgcaag gctttgacga cgtgttcctg accgactaag tcggcaaagg ttttcggacg
     2941 ccattttcgg gcgagaactt gataggccat gtttttctct tggtttcggt cgtgatgttt
     3001 ctgtcggtgc ttcggaatgc cgtctgaacg gcggtctcgg gcggcgtatt ctagcacttt
     3061 cggcttattg tccgcgcaag aacagtgcgt ccaattcttt caaagtcagt ttgacccacg
     3121 tcggcctgcc gtggttgcac tggttgctgc gcggcgtgtt ttccatatcg cgcagaagga
     3181 cgttcatttc gggcagggta agccggcggc cggcgcggat cgagccgtgg caggacatgg
     3241 tggcgaggat gcggttttcg tgttcctcga tggtttggct gctgccgact tgggcgagtt
     3301 cgcccaatac gtcgcgggcg agggagacga catcggattt gcccaacatt gcgggtgcgg
     3361 cgcggacggc gagggtgttg ccgcccatat cggataattc cagcccgaag cctgccagcg
     3421 tttcggcata atcggcaagg gcggcgcatt cttcgtggga cgcggcaaag gttacgggaa
     3481 taagcaggcg ttggctttgc agcctgccgt tttcctgacg ctggcgtttc attttctcgt
     3541 agttgacgcg ttcggcagcg gcgtgcatat cgatgagcag caggctgtct tcggcttggg
     3601 caagaatgta gatgccaagt aattgggcaa tggcgaaacc gagcggcggc agttcggatt
     3661 gggaggggat gccgtctgaa agcggcgtat ctgtttgggg agcaggcgtt tcagacggca
     3721 tattgccgaa acgtgcctgc tcgaatcggc ttaactcaag gtcgatgtcg tcggtttttt
     3781 tgtaaagctc ggcataggtg ttcattgccg cgcggctttc gcgcagggac aggctgcgtt
     3841 gttgcggcgc acgcgcggct tggtagggca taggcgcggt tttgcctgat gcaccaaagg
     3901 cattgcgcgt atcgggtttg ttgcctgtcg ggtagtcgga tgcgcgatta aacagatttt
     3961 cgctgtcgtt ttcagacggc attggtgcag gcgtaactcc cgtgatttcg tgcaacactt
     4021 cgcccgcgtt gccgacgctt tcggtcaggt cggcgcgcgt gtcggcaagg gctttgttga
     4081 gcgtgtggaa cacgagttgg tgtacctgct gactgtcgcg gaagcggatt tcggttttgg
     4141 tcgggtggac gttgacatct acggcttcgg gcggcaggtc gagaaagagg acgaaagcag
     4201 gtgtcagcgc gttgtgcaat acgtcgcggt atgcctgctt gacagcatgg agcatgactt
     4261 tgtcgcggac aaaacgatgg ttgacaaagc agtattgttt gtcggttttg cctttggcga
     4321 aagtcggctt ggcaatcgcg ccatagagcc gcaatgcgcc attgccgctg tcgatttcca
     4381 aggatgccgt ctgaaagtca tcgccgacaa tggcggcaat ccgttcatgc aggctttgtg
     4441 cagggagttt gaacacttgt ttgccgtcgc gtttgagtga gaaggcgatg tgcggatgcg
     4501 ccagagcaag tcgttcgagc atggtggcgc agtgggcgta ttcggtgttt tccgatttga
     4561 ggaacttgcg ccgcgcgggg gtattgaaga agagttcggc agcttcgatg gtggtgccga
     4621 cggggtgggc ggctgccgta gggctgctga gtttgccgtc ttcggctttg acttgggtcg
     4681 cgtgcgaact gtctttttga cggctggtca gggtcaggcg gctgacggag gcgatgcttg
     4741 ccaagccttc gccgcgaaag cccatgctgg caacgtgttc caaatcgttt aaggttttga
     4801 ttttgctggt ggcgtggcgg tggagcgcga gttcgatgtc gtccggatgg ataccgctgc
     4861 cgttgtcgct gacgcgaatc aggcggatgc cgccgcccgc cagttcgact tcaattgctg
     4921 ttgcgcctgc atcgatactg ttttcaacga tttctttcag ggcgttggca gggcgttcga
     4981 ccacttcgcc ggcagcgatt tggttgacaa gatggtcggg cagggcggcg attcgggaca
     5041 taaggcgggc ttccgttgca aaaaacgtct tattctataa taaaacccct tatctttctg
     5101 cccgtattct tgataaaagc cgtttatccc gtttggaaaa taccagtata atcacgccat
     5161 attccgtaaa attggagcac aaagatgtat cactaccaat ccgatgccac acaattcctc
     5221 aaccgcctga ttgaagaaaa acctgagttg gaacagcagc gtttggaaaa cagggggctt
     5281 ttgtgggatg tcgaactcaa tcccgaagaa caggaaaact tcgaggcggc aaaagtagcg
     5341 aaaaaacctt atacctatta ccaagactga acccgccgaa atgacgaccc atctgtccaa
     5401 tgtcgcaccg gacctgcaaa actatctgaa cagcatcggc gaacccgaac atcccgtttt
     5461 gacgcggctg cgtgagaaga ccgagcatca ccgtatgggc aaaatggcga ttgcgcgcga
     5521 acaggcggcg gttttggttt ggctggcaaa gctgattcgc gcggaaaaat atctggaagt
     5581 cggcgtgttt accggataca gcagcaccgc gcttgcattg gcgttgccag aacacgggcg
     5641 gattaccgcc tgcgacatca atgtaacctt taccgatacg gcgcgtcagg tttggaacga
     5701 ggcaggtgtg gcacataaaa tcagcctgca cctgcaaccc gcattgctga cattggatga
     5761 tttgattgta cagggtgaag ccggaagcta cgatttggca ctgatagacg cagacaaacc
     5821 gcccacgccg caatatttcg agcgttgcct caaactcgtc cggcaaggcg gcatcattgc
     5881 cattgacaat attttgttga acggaagggt gatgcgcgaa gcggcttccg acgcgccgcc
     5941 cagtgtcggc atcctcaaag atttcaatca aaacctgccg aacgacccgc gcatcgtccc
     6001 catcaccctg cccgtcggcg acggtttgac cctgcttctg aaaaaataat gaagaccaaa
     6061 ttaccgcttt ttatcatttg gctgtccgta tccgccgcct gttcttcccc tgtttcccgc
     6121 aatattcagg atatgcggct cgaaccgcag gcagaggcag gtagttcgga cgctattccc
     6181 tatcccgttc ccactctgca agaccgtttg gattatctgg aaggcacact cgtccgcctg
     6241 tcgaacgaag tggaaacctt aaacggcaaa gtcaaagcac tggagcatgc gaaaacacac
     6301 ccttccagca gggcatacgt ccaaaaactc gacgaccgca agttgaaaga gcattacctc
     6361 aataccgaag gcggcagcgc atccgcacat accgtcgaaa ccgcacaaaa cctctacaat
     6421 caggcactca aacactataa aagcggcagg ttttctgccg ctgcctccct gttgaaaggc
     6481 gcggacggag gcgacggcgg cagcatcgcg caacgcagta tgtacctgtt gctgcaaagc
     6541 agggcgcgta tgggcaactg cgaatccgtc atcgaaatcg gagggcgtta cgccaaccgt
     6601 ttcaaagaca gcccaaccgc gcccgaagcc atgttcaaaa tcggcgaatg ccaatacagg
     6661 cttcagcaaa aagacattgc aagggcgact tggctcagcc tgatacagac ctatcccggc
     6721 agcccggcgg caaaacgcgc cgccgcagcc atacgcaaac gatagttttt ccatttttcc
     6781 gctttttacc ccatccgcac caaggaggat ttatgataca aatcggcatc atcatgggca
     6841 gcaacagcga ttggcccgtt atgcggcagg cagcgcagtt tcttgaagag ttcggcgtag
     6901 aatatgaggc gcgcgttgtt tccgcacacc gcaccccgga tttgatgttc caatacgccg
     6961 aaaccgcacg ggcgcgcggc atcaaagcca tcatcgcggg cgcgggcggt gcggcgcatt
     7021 tacccggtat ggttgccgcc aagaccaccg tccccgtttt gggcgtaccc gtccccagca
     7081 aatacctgcg cggcgaagat tcgcttttat cgattgtaca aatgcccaaa ggcgtacccg
     7141 tcgccacatt cgccatcggc gaggcaggcg cggcaaatgc cgcattgttc gccgtttcca
     7201 tgctggccaa cgaaaacccc gaactggcgc aaaaactggc agacttccga gccaaacaag
     7261 aacagactgt tttgaatatg gaattggaac agatttaaaa cccgccgtcc gcgaatatat
     7321 agtggattaa atttaaacca gtacggcgtt acctcgcctt gccgtactat ttgtactgtc
     7381 tgcggcttcg tctccttgtc ctgatttttg ttaatccact atacaaatgc cgtctgaagc
     7441 cctgtttcgg gtttcagacg gcatttttgc gaacaacttt ttatgcctgt tctttttggc
     7501 gcattttttc tgccatcagt tcgtgcaggg ttttcttcgc cggtttcatc ggcacgcggt
     7561 tttgcgtcca acccaactgt ttgcgcgggg tcaggttgcg gaacttggtg gctgcccaac
     7621 cgaaggcgcg gtaggttttg ctgccgctga aaataccgtt gaatgtgcgc cacgccattt
     7681 gttcgccgaa ggtatgcgat gcgccttgcc ctcggatggg gtgcggcacg gtttcggtcg
     7741 gcgaacgttg cgcttcaacg cgcaaacgct gcatttgttc ggtaatcggg atgcgtaccg
     7801 gacagacttc cacacacgca ccgcacatgg tacaggcggt cggcagatcg cgggtggcat
     7861 caagacccaa caggtgaggg gaaataatct cgccaatcgg gccgggatag gttgtgccgt
     7921 atgccgcgcc gccgatgcgg gtataaaccg ggcaatggtt catacacgcg ccgcaacgga
     7981 tacattgcag ggtgcggcgc atttggtctt cggcataagc ctggctgcgg ccgttgtcga
     8041 gcagaaccaa gtgcatttct tgcggaccgt ctaattcctc actgcggcgc ggaccggtaa
     8101 tcatgttgaa ataagtggta atgttctgac cgatggcaga acgcggcagc agactgtaca
     8161 agggtggaat atccgacagc ttggcaacta ctttttcaat gccggtaatg gcgatatgca
     8221 cgggcggaac ggtggtactc aagcgaccgt tgccttcgtt ttccaccaga cacagcgtac
     8281 ctgtttcagc aacggcgaag ttcacgccac tcaaaccgac atcggcagtg ctgtaaatat
     8341 cgcgcagggc tttgcgggcg aagccggtca gttggtccac atcgtctgta agcggtgtgc
     8401 cgaggttttg gtggaacagt tcgctcacct gttctttggt tttatggatt gcaggcatca
     8461 cgatatgggt cggtttttcg cctgccattt ggacgatgaa ctcgcccaag tcgctttcta
     8521 ccgctttaat gccttttgct tcaagataat ggttcagctc gatttcctcg ctgaccatcg
     8581 atttgccttt gaccatcagc ttgccgtttt tggctgtgat gatgtcgtgg ataatttggc
     8641 aggcttcggt cggggtttct gcccagtgca ctttcacgcc caacttagtc aggttttctt
     8701 ccagctgctc cagcagggct ggcaatttag acaatgagcg ctgacggacg tgttcgcaca
     8761 aatcgcgcag gctttgcagc tcttcttcgt cggtcaaaac ggctttgcgt ttggtcatca
     8821 gcatatccat cgcggtacgc aggcttttgc gcaaaggctt gtcttgaagg gaaattgcgg
     8881 cgttttgctt gaaagtttcc ggcttcatgt gaaatttgat ggtttgcgta gtcatgcgtt
     8941 ttcctccaaa tcggcagggg aaatgtggtc gggcaggatg gcgaggatga ccaaatcgcg
     9001 cgggccgtgc gcgccgtaag caagcgtcag ttggatgtct gcggttttgg acgggccgga
     9061 aatcaggaat acattggtcg gcataccgtt ttccaccagt ttttcgcctt cgacggcatt
     9121 atgaaactcg ttgtacatct tggacgtatc gaacaggcag aaatgcacgg gcggaacgag
     9181 gcttaaagta cgcggttctt cggggctgga aaacagcatc agcgtgccgg tgcgggcgat
     9241 gccgcattgc gcgccgctga agcccgcatc gatgttcgtg aaaaactcgg ttttccaagt
     9301 atcgatttcg cgctcgaagg caatcggttc gatattgctg tccgccaatg cggcacgggc
     9361 aatttgtccg tgttcggtcg ccaagggcag caggatgttt ttcaaaccct tgccttctgc
     9421 cgcttcgcgg aaaacctgca tccaattgct tttcgtcacc caataaattt cggttttgac
     9481 cgcacgcata gcggcagccc aatgtttcag acgctcaact tcgctgcccc aagaaacacc
     9541 catttcacgg taataatcaa aaaccgcagg ttcttccatc ggcaatgcgt cggctttttt
     9601 cagttttgcc aaaatatttt cacgcgcgct catgctttgc ctccggtgcg ttccaacaag
     9661 aaggatgcga tatgtttcgg acgcggcata tccggctcgt ccttggcgat tttgccgccg
     9721 atgttcatca tacagccgca gtccgcgctg atgatttcgg ttgcgccggt ttctttcagc
     9781 gcggcgactt tgtctgttac cattgcgccg gaaatatcgg cttgtttgac ggagaatgtg
     9841 ccgccgaagc cgcaacattc gctttcgtgg tcgtggacga tgcgttcgac gttttccata
     9901 ccgtcaatca gttgccagcc tgaaagatgg acattcattt cgcggcgggc ggcgcaggaa
     9961 gtgtgaacgg cgactttgag cggttcgccc ttgtcttcgg gtttgaaacc gatggcaagc
    10021 aggaaatggg taaactcgat gatgcggccg gcgcaatcca cagccctttc ctcgtactcg
    10081 ctgcctttaa acagcgtcgg ccagtggtgt ttcatcatgc cgccgcacga gccggacggc
    10141 acgacgatcg gccagttttc agggaaaagg tcgagttgtg ctttggcgac atcgaaggct
    10201 tcggtcggat ggccggatga ataggcaggc tggccgcagc agctttgcgc catcgggaaa
    10261 tggacgcgta tgccctgctg ctcgattagg gtaatggcat ccatgcctgc ttcgggcata
    10321 aaaagatcaa ggacgcaagt gccgaaaaaa tagacatcgg tcggattgct gtcgtatcgg
    10381 atgatttttg ggggaatggt tgcgctcatg ttttttgata acgggaaacc cgtttttctc
    10441 tgtagaaagg taagcgttta ctttaagtaa ttgactgttg cgggtcaagt ctaattttaa
    10501 aaaataatcc ggtttttctt acaaactgcc cccataacgc ttactgtacc ttaatctgat
    10561 ggttttcgat aataattatc attacaatgc aatgccggtt cgtttgcttg tgaacattca
    10621 agatgccgac tctgacggca ttcagacagc atctgaaaac aataacggca caaggatggg
    10681 gtacaatcgc cctatggaaa aaaacaccct atccgcccgc gcaccatccc cttggctgcc
    10741 gcttctgctg gcaattgcca tttttatgca gatgttggat gcgaccattt taaataccgc
    10801 actgcctgaa attgccgccg acctgaatga gtcgcctctg gatatgcaac tggcagttat
    10861 ttcctacacg ctgacggttg ccctgctgat tcctttgagc ggttatttgg cggacaggtt
    10921 cggaacgaaa aaagtctttt tcggttcgat tgccgttttt atgctcggat cggcattgtg
    10981 cgccgcatcg ggttcgctgt ttgaattgac gctttcccgt gtcgttcagg gcatcggcgg
    11041 ttcgatgctg gttccgatac cgcgtctgac catcttgcgt gtgtacgaca agtccaagct
    11101 gctcaatgcc atcaattatg cggttatgcc cgcattaatc gggccggttt tagggccttt
    11161 ggcgggcggt tatttggtcg aatacgcttc gtggcactgg attttcctgc tcaacctgcc
    11221 catcggtctg ctgggtttca tattgggacg caacatcatg cccgatatta aaggcagtaa
    11281 tatctcttta gacttcaaag gttatctgat tttttctgcc gccgcgtgcc tcttgttact
    11341 ttcggcagaa agcctgtcgc acgcgctgcc tccgtatttt gcactgttgc cgctgtgcgg
    11401 cggactgctg tttgcacgcc gttatttccg acatatgaaa accgcgtcca aaccgattta
    11461 ttccgccgac ctgtttctga tacgcacttt ccgtctggga ctggcgggca atctgttcag
    11521 ccgtctcggc atcagctcga ttccttttct gatgcccctg atgtttcaaa tcgctttcgg
    11581 cttcggcgca agcctgtcgg gttggctggt cgcacccgtc gccctgtctt cgctgctggt
    11641 caaaccgctg attgcaccgc tcatgaaacg tttcggctac cgcacggtac tgctttggaa
    11701 caccaagctg cttgccgcct tcatcatgct gctcgccctg cctgacggaa actcgccgct
    11761 gtggatttgg gttttcctct cgctggcgat cggcgcgtgc aactccctac agttttctgc
    11821 catgaacaca ctgaccctcg ccgatttgcg cccgcaacaa acaggcagcg gcaacagcct
    11881 gatggcggtc aaccaacagc ttgccatcag catgggcatt gttgccggcg cattaatcct
    11941 taaaaactgg acatttctga taccggcttc ttcaggtctg cattccgcct tccgtatgac
    12001 cctgctcagc atcggcggca tcacccttgc atcatcgctg gttttcaaac ggctgcacgt
    12061 ttcagacggc accaacctga cacggaacac accgtcctga agcggtccac acgcaaaact
    12121 tttacccgtt tcaacgtttg gattatgata ccgcacttcc atgcgcgcca accccaaaac
    12181 acaggcaatg ccgtctgaaa ccatatccct gatgaaaaca cgcagcctaa tttccctttt
    12241 atgcctcctt ctctgttcat gttcttcatg gttgccccca ctggaagaac ggacggaaag
    12301 ccgtcatttc aatacttcca aacccgtccg cctggacaac atcctgcaaa tccggcacac
    12361 ccctcatacc aacgggctat ccgatatcta tctgttgaac gacccccacg aagcctttgc
    12421 cgcccgcgcc gcccttatcg aatctgccga acacagcctc gatttgcaat actacatctg
    12481 gcgcaacgac atttccggca ggctgctgtt caacctcgtg taccttgccg cagaacgcgg
    12541 tgtgcgcgta cgcctgctgt tggacgacaa caacacgcgc ggattggacg acctcctgct
    12601 cgccctcgac agccatccca atatcgaagt gcgcctgttc aaccccttcg tcttacgaaa
    12661 atggcgcgca ctcggctacc tgaccgactt cccccgcctc aaccgccgca tgcacaacaa
    12721 atcctttacc gccgacaacc gcgccaccat actcggcgga cgcaatatcg gcgacgaata
    12781 cttcaaagtc ggtgaggaca ccgttttcgc cgacctagac atcctcgcca ccggcagcgt
    12841 cgtcggcgaa gtatcgcacg acttcgaccg ctactgggca agccattccg cccacaacgc
    12901 cacgcgcatc atccgcagcg gcaacatcgg caagggtctt caagcactcg gatacaacga
    12961 cgaaacgtcc agacacgcgc tcctgcgcta ccgcgaaacc gtcgaacagt cgcccctcta
    13021 ccaaaaaata caggcaggac gcatcgactg gcagagcgtc caaacccgcc tcatcagcga
    13081 cgaccctgca aaaggactcg accgcgaccg ccgcaaaccg ccgattgccg ggcggctgca
    13141 agacgcgctc aaacagcccg aaaaaagcgt ctatctggtt tcaccctatt tcgtccccac
    13201 aaaatccggc acagacgcac tggcaaaact ggtgcaggac ggcatagacg ttaccgtcct
    13261 gaccaactcg ctacaggcga ccgacgttgc cgccgtccat tccggctatg tcaaataccg
    13321 aaaaccgctg ctcaaagccg gcatcaaact ctacgagctg caacccaacc atgccgtccc
    13381 tgccacaaaa gacaaaggcc tgaccggcag ctccgtaacc agcctgcatg ccaaaacctt
    13441 cattgtggac ggcaaacgca tcttcatcgg ctcattcaac ctcgaccccc gttccgcacg
    13501 gctcaatact gaaatgggcg ttgttatcga aagccccaaa atcgcagaac agatggagcg
    13561 cacccttgcc gataccacac ccgaatacgc ctaccgcgtt accctcgaca ggcacaaccg
    13621 cctgcaatgg cacgatcccg ccacccgaaa aacctacccg aacgaacccg aagccaaact
    13681 ttggaaacgc atcgccgcaa aaatcctatc cctgctgccc atagaaagtt tattatagaa
    13741 atatagtgga ttaataaaaa ccagtacggc gttgcctcgc cttagctcaa agagaacgat
    13801 tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact gtctgcggct
    13861 tcgtcgccta ttaatccact ataccgtctg aaacaccttc agacggcata tccgaacccg
    13921 caaaggaaaa accatgtttc cccccgacaa aacccttttc ctctgtctca gcgcactgct
    13981 cctcgcctca tgcggcacga cctccggcaa acaccgccaa ccgaaaccca aacagacagt
    14041 ccggcaaatc caagccgtcc gcatcagcca catcgaccgc acacaaggct cgcaggaact
    14101 catgctccac agcctcggac tcatcggcac gccctacaaa tggggcggca gcagcaccgc
    14161 aaccggcttc gattgcagcg gcatgattca attcgtttac aaaaacgccc tcaacgtcaa
    14221 gctgccgcgc accgcccgcg acatggcggc ggcaagccgc aaaatccccg acagccgcct
    14281 taaggccggc gacctcgtat tcttcaacac cggcggcgca caccgctact cacacgtcgg
    14341 actctatatc ggcaacggcg aattcatcca tgcccccagc agcggcaaaa ccatcaaaac
    14401 cgaaaaactc tccacaccgt tttacgccaa aaactacctc ggcgcacata ctttctttac
    14461 agaatgagcc gcccgccgcg ccgcgccgga atcgcggcaa aatgccgcct tattccgctg
    14521 tttcggtcag cgatgagaac acgtcgaaat aagtcgggaa ggttttgtgg gtgcatttcg
    14581 gatcgttgat gacgacgggt acgtccaaca gcgaaaccag cgagaaacac attgccatgc
    14641 ggtggtcgtc gtaagtgtcg atgacggcgt cgggtgtcgg cgtttcgggc ggggtgatgt
    14701 gaattgcttc ggcttcttcg gcgacttttg caccgagttt gcgcaactcg ttcgccattg
    14761 cggcaatgcg gtcggtctct ttgacgcgcc acgaaccgat gttgcgaagc gtgcaggttt
    14821 gccctgtagc aagcgcgacg acggcgaggg tcatggcggc atcggggata tggtttgcat
    14881 ccaaatcaaa ggcttggacg gcacgttctt tcgggcgcga gacttcgacg aagttttcgc
    14941 cccaaaccac gtccgcaccg attttttcca gctcgcaggc aaaggcgaca tcgccctgta
    15001 tgctgtttgc gccgataccg gtaacgcgga cgggcgtggc ggcaatcaaa ccggctgcga
    15061 ggaagtagga cgcgctggag gcatcgcctt cgacgtgcaa gtgttcgggc gcgtggtagt
    15121 gcgcatcggc gggaattttg aagacgcggt agccttcatt gataacctgt acgccgaatt
    15181 gcgccatcag ttttaaagta atgtcgatat agggcttgga aattaactcg ccgaccatac
    15241 ggatttcaaa cgcctgcccg gtcagcggca acgccattaa aagggcggtc agaaactggc
    15301 tggatacatt gcctttaatc ggaatcacgc gctcgccgtt gtcttggcgt tcgccgatat
    15361 gaagcggcgg atagtgttcc ttgccgagat attcgacatc ggcgccggca atccgcagtg
    15421 catcggccaa atcgccgatg gggcgttcgt gcatacgcgc aacgccgtgc aggcgataat
    15481 cgccgcccaa aacggcaagc gcggcggtca gcgggcggaa cgccgtgccc gcgttgccca
    15541 aaaacaaatc ggcagtgcgg ttggggaagc gtccgcctgt gccgtgcact ttcagacggt
    15601 cttcggcaag atattcgatt tcaacgccga gtttatcgag tgcttcgagc atacggtcgg
    15661 tatcgtcgga tttgagcagg gaatggattt cgcaagcatt gtcggacaag gcggcaagca
    15721 gcagggtgcg gttgctgatg cttttggagc cgggcagggc gacagtggaa ggtttgagcg
    15781 aggcggcagg gaggcggagg gattcggtca tggcaaaacg taaatataaa gataaaaaca
    15841 gcctgcatta tactggtgca aatgccgtct gaaaaatctc aggtttggca ttttcggttt
    15901 taaagtccgt gaatatggtt ttttgtgccg aaaattgatt atttttaaat tttttgtttc
    15961 taaaattttt ttgccggcat attttcggct tttgttgcgg acatagcgtg tttgcggacg
    16021 gcacggcacg attgccaatt tcatagaatt tggtagaata gccgctgttc aacgacagac
    16081 aagccgccga ttttccgggc ggcttgtgtt tttattttaa tatccggacg cacaaaacaa
    16141 gacggtatgc ggtatgtttg ctgccgcaaa ccggtgtgcc ggaatatgga gaaaaaagac
    16201 cgatgcaaaa aatccccctg accgtacgcg gtgcggaatt gctgaaacag gaattgcagc
    16261 agcttaaaag cgtggcgcgc cccgaagtga tcgaagcgat tgctgaagcc agatcgcacg
    16321 gcgatttgtc cgaaaacgcc gaatatgaag ccgccaaaga acgccaaggc tttatcgaag
    16381 gccgcatttc cgagctggaa cacaaacttt ccgttgccca catcatcaat ccgaccgaaa
    16441 tccacgccga aggcaaaatc gtgttcggta cgacggttac gctggaagat ttggaaacgg
    16501 aagaacacgt tacctaccaa attgtcggcg aagacgaagc ggatatcaaa cagggcaaaa
    16561 tctatgtcgg ctcaccgatt gcccgcgcct taatcggcaa ggaagagggc gatacggcgg
    16621 aagtccaagc cccgggcggc gtgcgcgaat acgacattat cgaagtccga tatatttgat
    16681 tcggcttgat ttcgatacac ccgacacacg caggaaatta tagtggatta ataaaaatca
    16741 ggacaaggcg acgaagccgc agacagtaca gatagtacgg aaccgattca cttggtgctt
    16801 gagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ccggtttttg
    16861 ttcatccact ataacagcaa ccctgtcgcc gtcattcccg caaaagcggg aatctaggac
    16921 gcagggttaa gaaaacctgc atcccgtcat tcctcaaaaa cagaaaacca aaatcagaaa
    16981 cctaaaatcc cgtcattccc gcaaaagcgg gaatccaaac ttgtccgcac ggaaacttat
    17041 cggataaaac ggtttcttag attccacgtt ctagattccc gcctgcgcgg gaatgacggc
    17101 agaacggttt ctgttttttc cgataaattc ttgaggcatt gaa
//
LOCUS       DAWFKN010000037        17071 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFKN010000037 DAWFKN010000000
VERSION     DAWFKN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.037"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(27..653)
                     /locus_tag="WGR46_001197"
     CDS             complement(27..653)
                     /locus_tag="WGR46_001197"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0856142.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR46_001198"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR46_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0856143.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR46_001199"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR46_001199"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0856144.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WGR46_001200"
     CDS             complement(3056..3553)
                     /locus_tag="WGR46_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0856145.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR46_001201"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR46_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0856146.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR46_001202"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR46_001202"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0856147.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WGR46_001203"
     CDS             complement(5863..6309)
                     /locus_tag="WGR46_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0856148.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WGR46_001204"
     CDS             complement(6314..6673)
                     /locus_tag="WGR46_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856149.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WGR46_001205"
     CDS             complement(6746..7474)
                     /locus_tag="WGR46_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0856150.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR46_001206"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR46_001206"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0856151.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8623..9333
                     /locus_tag="WGR46_001207"
     CDS             8623..9333
                     /locus_tag="WGR46_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0856152.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVLFPFAGRDLTLTRLPSTSRTYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            9416..10618
                     /gene="trpB"
                     /locus_tag="WGR46_001208"
     CDS             9416..10618
                     /gene="trpB"
                     /locus_tag="WGR46_001208"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0856153.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWAEFRCDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGR46_001209"
     CDS             complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGR46_001209"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0856154.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAKLAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAAAQPQAQAAAQPQAQAAAQADAVSTNTNSALSDAMASTQ
                     SILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGID
                     RSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLR
                     SRLQTQQKANFNRTSIQTGFTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKV
                     SSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQ
                     YSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         3468 a         4187 c         4646 g         4770 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcgactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 accgcttccg ctccggtgat gcggacggct tcgccgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccagcagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg gtggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtcgaact ggcggcagaa tgtgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggcacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg atttttttgg cgcgggcaat atcgacggcc
      541 cggctgctgc cttggaaaaa gaccagcccg acggcatctg cacctgccgc tacggcggcc
      601 gatgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacgat gcgtttgcac cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgttcaaaac
      781 cacttcgtcg ccttcgcgcg ctttaatcag gcaacgcgcc aacggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacaggccga cggtcgcgcc gaaaaatact tggtcggtcg cttcgcgcaa
      961 ttcgggatcg acgacgacgg cggactccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag atagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttgttga ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcgga ttgagtgctt tgacgacggc
     1501 ttccaaatcg ctcaaatttt ggaacacgat gccgtctgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgctcgttgg caatcaactc ttcgcgcgtg ggcatatcaa tgccgtacac
     1621 attgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgcccgccgc
     1681 gcgtaccatt tcgacgattt cgcggctggt cgtcccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctgtccgg gcataataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 accctcgcgg tagggtttgt cgagatggac ggcaagctcc atcgcgctgg ggcggctggt
     1921 gtcggggatg ggcatcacaa cgtcgatgcc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccagcgac acgcccatat ccaagcgcga ttggtaaacg gatacgccgt caatcacaga
     2041 gtcggggcgg gcaaaataaa catattcaaa aaggcagggg ctgagtttgg cacggtcgct
     2101 gcattggcgg gcaatcattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 gtcgcgttcc aaatcgtagg taagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggattttctg ccttcgctgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacgccgt atgcgccgcg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactt cgcggcgcaa ttcgtgcgcg aatacgttga gcaagacttc
     2461 ggaatcggag ctggtgttga cgtggcgtag gtgtttgttg cacacgtttt cataaagttc
     2521 ggcagtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaacggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgcccgc gttgcccgca gtaggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgtgtg cggaacactt cgcgcaccat
     2701 gcctttgcct ttgtgcatat ggaaggtacc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagagaat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccgg agtttccgcc gtgccgccgc tatggttcaa cacggcttcg gaaagcgata
     3121 cgaaaaacgg cagtgtgtaa gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca aacattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgccgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaagtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ccgtctgaaa
     6301 ggcgcgcaca gccttacatc atgttgtcga acacgggggt aatgttcaat tccgcttctt
     6361 ccatgtccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttatcga tgtcccaacc tgccagctcc gcctcgaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggctcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggatat gctgtctgaa
     6721 cgttcggttt tcagacggca tagcatcaat gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat cgtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgttgct gctggccgcc ggaaagttgg gaagggaagg
     7081 cgtctttttt gtgtgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctctgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt aatgttttcc aacacggtca
     7201 ggtgcgggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcttca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gctacgtatt tttccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tactcgaaat cgtccgccct gccgatgcgg
     7861 tgtttcaccg gaatcatgcg gacgacggcg gaatcgactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgataatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttccttttc ggttgaaacc ccgcccttta
     8461 gggcggtaga atcagactct atttgggagg ggcgtaaccc cttccaaatc aggatggcac
     8521 atagggcggt gctttatgtg tcgtcctgtg tgttgaaaca taaatgtgtt tacagtatcc
     8581 gtttgatgtc ggcattgtaa ccgaaaacgg cagggcgtga taatgctgtt tgaaggcttg
     8641 ccgtgtttgg cggtttggtg caaaaaccgg ctgtctgccg ttttgcctgt tggaggattg
     8701 aacgtgtctg aaaatctgct tgaaatcgaa acccatccct tcgattccgt gttgccgccg
     8761 aaggctgcgg tcatgatgat ggggacgttt ccgcccaagg aagacaaacg cgcgatgcag
     8821 tttcattatc cgaatttcca aaacgatatg tggcgcgttt acgggctggt gtttttcggc
     8881 gatgcggatt atttcaaagt gccgtccaac ggggaggctg cgcactctga aaaagcgttt
     8941 gatgccgaga aaatcaaggc gtttttgcac gaacggggga ttgcgtcctg tccgaccgtt
     9001 ttgaaggcgg tacgtcagca cggcaatgcg tccgacaagt ttttaaaggt agttgaaacc
     9061 gtcgatttgg cggcggtgtt ggcaaaaata cccgagtgcc gccatatttg tacgacaggc
     9121 ggcaaggcga cggaaatcct gctcgatatt cagggtggcg gcatcaaaat gccgaaaacg
     9181 ggcgaaaccg tgctgtttcc gtttgccgga cgggatttga cgctgacgcg cctgccttcg
     9241 acttcgcgca cctatccttt gagtttggcg aaaaaagcgg cggcgtatcg ggcgtttttc
     9301 gaaatggcgg gcttgtgtga aaagcagtta taattgccga caatttcccg ttcggacggc
     9361 atgtttgaaa aaacggaaat gccgtttgaa aacctgaagc acaaggaaga atccgatgaa
     9421 gaactaccac gcgcccgacg agaagggctt tttcggcgaa cacggcgggc tttatgtttc
     9481 cgagaccctg attcccgcct tgcaagagct ggcggatgcc tataaggcag cgaaaaacga
     9541 tcctgaattt tgggcggagt ttcgctgcga tttgaaacat tatgtcggca ggcccagccc
     9601 cgtttaccac gccgcgcggt tgtccgagca tttgggcggc gcgcaaatct ggttgaagcg
     9661 cgaagacttg aaccacaccg gcgcgcacaa agtcaacaac accatcggtc aggcactgtt
     9721 ggcaaaacgc atgggtaaaa aacgcgtcat cgccgaaacc ggcgcgggtc agcacggcgt
     9781 ggcgagtgcc accgttgccg cacgcttcgg tatgacttgc gacgtgtata tgggcgcgga
     9841 cgacatccaa cgccaaatgc ccaacgtgtt ccgtatgaaa ttattgggcg cgaacgtggt
     9901 cggtgtagaa agcggcagcc gcacgctgaa agacgcgatg aacgaagcca tgcgcgaatg
     9961 ggtcgcccgc gtggacgaca cgttctacat catcggtacc gccgccggcc ccgcgccgta
    10021 tcccgaaatg gtgcgcgatt tccaatgcgt gattggcaac gaagctaaag cgcagatgca
    10081 ggaagccatc ggcagacagc ccgacgttgc cgttgcctgc gtgggcggcg gatcgaacgc
    10141 catcggtttg ttccacccgt atatcggcga agaaaacgtg cgcctcgtcg gcgtggaggc
    10201 tggcggtttg ggcgtgaaca cccccgatca cgccgcgccg attacttcgg gcgcaccgat
    10261 tggcgtattg cacggtttcc gcagctatct gatgcaggac gaaaacggtc aggttttggg
    10321 tacgcactct gtttccgcag gcttggatta ccccggcatc ggcccggaac acagccatct
    10381 gcacgacatc aagcgcgtcg aatacactgt tgccaaagac gacgaagcac tcgaagcctt
    10441 tgacttgctc tgccgcttcg agggcatcat ccccgcgctc gaatccagcc acgccgttgc
    10501 ttgggcggtg aaaaacgcgc cgaaaatggg caaagaccaa gtgattctgg tcaacctatc
    10561 cggtcgtggc gacaaagaca tcaataccgt cgcgaagctc aaagggatta aactgtaacc
    10621 tcgtccgtct gatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta
    10681 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
    10741 atgccgtctg aagcctgagt tcagacggca ttttattttg ctatgaattt agtattttag
    10801 aaacgaatct gtattttaat ttgtccggat ttttgttttt ccaattgttt tccttttgta
    10861 atactgccat ttacgtttaa tgtaacatta cggtacagta acgcggcacc tgctgaatat
    10921 tgctgttgat tatctgcttt atagacgaag gaattaccgc ccacattcac gccgcctttg
    10981 ccataattgg caaagtaagc tgcagataac aagggtttta cggtaaggtt gccgacttta
    11041 aaccgataag caaaatccag tccggccgtt agtgttttca ctgccataga acttacttta
    11101 acactgtcgt tacccaactt gtaatctgca gatgacaggc ggctgtaacg gattcccgca
    11161 ctggggacaa tctcgaattg attgattttc agcgtattgc ccaaagtaaa gccggtttgg
    11221 atgcttgttc ggttaaagtt tgctttttgc tgcgtttgta accggcttct caagctgcct
    11281 gcaccaatat cgccggccac ataccaagca tcatttaaat aatacttacc ataaaggttg
    11341 gcttgcacaa aagtattttt gccgctcgcc tgatcaaaag tatgctgact gtcagagtaa
    11401 gtcaatacgc cgcctatctg catattttcg gacaagctgc ggtcaatgcc gatttgtgtt
    11461 tgcgtgcgtt tcgaactaaa ccggcgatat tgtgcggaag cataatcacg gccataaccg
    11521 atgttcgaca tccaaacgct gtttttttcg gcatcagcac gtgatttttg tgcaatgtgc
    11581 cgtgttaatg aagcacctgt atccaacaag atagattgcg tgcttgccat tgcgtcagat
    11641 aaagccgagt tggtattggt gctgactgca tcggcttgcg cggcggcttg ggcttgcggc
    11701 tgagcggcgg cttgggcttg cggctgagcg gcggcttggg ctcgcggctg agcggctctt
    11761 ggttgcaaac tcactgtctc aactttttca tgaagttccg tattgtcttg aggctgtttg
    11821 tctgatgtgt caaccgattc ggatacatct tcatcttcca acgcatccaa ggggatttct
    11881 tcataatcat tttcatattt ttcatgaagt tccgtattgt cttgaggctg tttgtctgat
    11941 gtgtcaactg attcagatac attttcatct tccaacgcat ccaaggggat ttcttcatag
    12001 tcattttcat accagtccgg attatgcaag gccctgggtg ctgcgtaagc tgacgaatca
    12061 aatgatggcg agggcggtgc cggcagagta gatctgcgtc cgcgacgttt cggacgattt
    12121 tgggaagctg ccatataatc ctgaggtgcg gcacggcgtt ttcggcgttg cggctgggat
    12181 tgggctgctt gacggtgctc ttcttcctca gcttttcgtt tcgccgattc tgccgcttct
    12241 cgatctgttt cggctttttg ttttgccgac aactctgctg cttggcgttc cgcttcttga
    12301 cgacgtgcaa gttcggcggt ctggcgagct tcttgctggc gttttgctgc ttcggctgct
    12361 gccctttgct tggcaagttc ggcggttttg cgttcggttt ccgctttttg tttgacagct
    12421 aactctgcag cttttcgtgc ttcttcctgt tgacgcgcaa gggctttttg ttggcgtgcc
    12481 gccaatgctt gggcttcagc tgcggctttt tggtttgccg acaattctgc cgctttgcgt
    12541 tctgcttcct ggcgatgtgc aagttcggca gcttggcgtt ttgcctcttc ggcttctgct
    12601 tttcggcgcg cagccaatgc ttgagcctca cgttcggctt ctgccctttg ttttgccaac
    12661 aactctgccg ctttgcgttc ttcctgctgt cggcgcacaa gttctgcttg ggcttgggca
    12721 atttccacat tgttttgctg taccgaacgg tgtccgcggc gtttaggacg gtcttgggaa
    12781 gctgccatat aatcctgcgg tgcggcacgg cgtttgcggc gttgcggctg ggattgggct
    12841 gtttgacggc gttcctcttc cccgaccctt tgttttgccg acaactctgc cgcttcgcgt
    12901 tctttttcat gccggcgtgc aagctctgca gcttggcgtt ttgcctcttc ggcttctgct
    12961 tttcggcgta ccgccaatgc ttgagcctca cgttcggctt cgactttttg ttttgccgac
    13021 aactctgccg cttcgcgttc tttttcatgt cgacgtgcaa gttcggcgct gctgcgttct
    13081 tgttctgctt tttggcgggt cgccagctct cttgcttcgc gttcggcttc tgctttttgt
    13141 tttgctaact ttgccgattt acgctctgct tctgcttgct gacgcttcac ttgctccgct
    13201 tttgcttgct ggcgttttgc ttcttcggct tgatttgcct gcgggctagg cggtgcgacg
    13261 acgatatttt gaggcttggc aatttgtgca ccgtccgttt gtgttgcctt ttgtgcttga
    13321 gaagccgtgt ttgtggcagg agacggggcc ggtttgactc ggcggcggtt ctcggcataa
    13381 ggattgtaca atcgggtaat gccattttct gttttgattg tataacgcaa tgcgcctaaa
    13441 tctacatggt tattcgccaa ggaaacagaa aggcgggagc ggtcttgtac ggatgatgca
    13501 tcaaagagat tcagcccttc ctgattgggt tcgcctgttt tatcttgaac atggagctga
    13561 taatgaccgg atgcggattc cttcacaagc actttatccc caagattttc cgccaagtgc
    13621 gtcagataat gaaaatgccc gttaccggat aaatgattga ttttgagcgt gtggtattga
    13681 tttgcacttt gcgcatcgga ggcattgttc aaatgaatat ggctatccgc caatgacaga
    13741 ttgtgtactt ggctgtcgcc agtcaaatgc catttgctat gttggtttag gctgacacgg
    13801 ctgtttcctt gcccttgaat ttgcccccat aatgcagcct tgcccaagac caatgccgca
    13861 ttctggttca gattcacatt gccgttaatc tgtgtcgctc caaagctgtt taaagcctta
    13921 tcggataagt tgcctgtgtt gcaggtaaca taaccggtat agtccgagcg cacgcaaacc
    13981 tcatcgccgt ttttgtaacc caaatttact ttggcgttgt ctgtcgcggt gatgttggcg
    14041 gtaatgttgg atacatttct accggaagag aatgatgcgg attgattaac cgcaatttct
    14101 gcggctttga atgtgcggtt tatccagtcg tcttcaaata cgacttcatt gtttttggag
    14161 aaatgtgcgt cttttcgggc tgaagatttg ttcacaaaat ctcttgcgtg tggtgttgga
    14221 cgacctgata acaagacatt gccttgagtt acgcttattt ttccgtttaa attagtgccg
    14281 cctgttaaca agaaacggtt ttgcgcgctt ttgccgttga aattaaggtt taatgcaccg
    14341 ttatgtcctt ttccgttttc ttcgccaaag aaaccgctaa aaccgctaat acgctgattg
    14401 tttttgtggt tcatcgcgtt tttcttggct tcttcttgcg tatatcccat aaagacccag
    14461 tcgttatttt ctgttacacc gttctcgggc atcggtgcgt tcacgctgcc gccggatttt
    14521 agggcgtaat aacgatagtt tttgtaataa aggtctttgc cttgcggaat cgggcgcctt
    14581 ggtcggtagt aataactata atcatcttcg tcatagtcat tttgtatgct atgtaccgaa
    14641 aggctgttcg gattggtaat taaagattta cccgttagcg tgattgtgga ggcgtggctt
    14701 gtgttgtggt tgacaatgcg cgcgccttca tccacgttgc ggatgtgttc aaaagtcaag
    14761 tcattgccat tggcatccaa acgaccgcca cggaaaccga aatataggtt atcgggatta
    14821 atctgatttg aactatttaa taccaatgta ccgcgtccgc tgacaatgcc gacttgggag
    14881 aaagcctgga cttttttgtc ggcatcggct tgttgattca gaataaccgt accgtcgccg
    14941 acttttaatt gcccttggtt aacgcctgtg ccgtttattt ctaatgtgcc tttgccgatt
    15001 tttgccaatc tgtcgccatt cggatttttg acttgccaaa cgactttttt gccgtcggta
    15061 acatcaatcc ccgcacctag ccaagtgatg tcattattta tacctttgac tgtgtaatcg
    15121 cctttgaaaa acagaccgcc cgcgccttgg ttgatgtttt gatccaatac caaagtgccg
    15181 ttgtcttcaa aggtaacatt ctgtccgttg tttgcatcct tttcattgcc ggcaagcctt
    15241 accgctgtag aaccaatctt gctgtttgtg ccaaaagtaa tgttccaatg atgttctcca
    15301 ttacctttga tggtgccggc gttatcgcgt tgtttgattt tatctgcaaa ttctttttta
    15361 tagatattcc attcttgcca agagtttttt tgatagcctg cccaataatc gtaagcacct
    15421 aaaaagaccc agcgtttttc ttgtttatca taagcaaata atggtgaacc gctatcgcct
    15481 aacacagcat agttagtcaa tgcgttttga gataaaactt ctttgagttt ttctggggaa
    15541 tgatgttttg aattatcacc gaagccaatc aagccttctt gatttagatt cgatgtgaca
    15601 ttcacgtctt gataaggcgt acctgcaatg gcataacgat aagctcgtga aagctctgtc
    15661 atattgtagc ggctgttata ttcaaattgc gtacctgctc caactcgcac aaactcagaa
    15721 aaacggtttt tatctttata ggtttcaacg ccgccgcctg cactggttgg tgcaataggt
    15781 gcgacttctg ttacgaattt attaaggcgt gccatgttgt agtcttcaag acgaccttga
    15841 ttaccgtgat gccaattttt atttggttcg tagtcatttt gtgcaactga acgatattcg
    15901 ttttcatcat tgctaacatc taagtgccca ttgtgatgcc cgtaataaga gatttcgtct
    15961 ccttttacat gtttgacgct gacggcatac tggggatcga tgacggttaa tgtacgtctg
    16021 ttgacatctg caacgctaaa atcaatcatc ggtacgttgg ataatgcgtt gccgatgttt
    16081 tgaccttgtt tgtttttcac tgataaatcg gttgcgccga caaaaaattt gcctttgttt
    16141 tctgcaaagt cacggaatat ttgataatcg acatcgtctc tgaccaatgc cgcttctgag
    16201 tatggcgtaa gggcataggc aagaaagatg gataaggata tggcgttaat tttaaaacgt
    16261 ttggttttca taaggtttta ccgttttaag ggtgataatg ttttgtattt tacgccggtt
    16321 tggcggtgtt gactattttt tgttgtgtga atcattaatt ttggtgtttt attgccaatt
    16381 taaaaaaaga atcccgatgt ttttatttcc gcttcctttg ttctgttatt caagcgaagg
    16441 cgggaagccg gttttcgggg ttcggttctt ccgttaaatt tctgcggctt tttgtttttg
    16501 gattcccgct tttgcgggaa tgacgggatt ttaggtttct gattttggtt ttctgttttt
    16561 gagggaatga cgggatgtag gttttcttaa ccctgcgtcc tagattcccg ctttcgtggt
    16621 aatgacggga tgtgggttcg tgggaatgac gcggtgcagg tttccgtacg gatggattcg
    16681 tcattcccgc gcaggcggga atccagacct tagaacaaca gcaatattca aagattatct
    16741 gaaagtccga gattctggat tcccactttc gtgggaatga cgaaaagtgg cgggaatgac
    16801 gaaaagtagc gggaatgacg aaaagtggtg ggaatgatgg ttcgggcatt ccttaaatcc
    16861 cccgtgtatc gctgtaaatc ttagagatgg cggaatatag cggattaaca aaaaccagta
    16921 cagcgttgcc tcgacttagc ttaaagagaa cgattctcta aggtgctgaa gcaccaagtg
    16981 aatcggttcc gtactgtttg tattgtctgc agcttcgccg ccttgtcctg atttttgtta
    17041 atccactata aagaccgtcg ggcatctgcg g
//
LOCUS       DAWFKN010000038        16589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFKN010000038 DAWFKN010000000
VERSION     DAWFKN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.038"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            209..1273
                     /locus_tag="WGR46_001210"
     CDS             209..1273
                     /locus_tag="WGR46_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0856155.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WGR46_001211"
     CDS             1414..1737
                     /locus_tag="WGR46_001211"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0856156.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WGR46_001212"
     CDS             1809..2132
                     /locus_tag="WGR46_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0856157.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGRQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WGR46_001213"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WGR46_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0856158.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WGR46_001214"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WGR46_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0856159.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WGR46_001215"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WGR46_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0856160.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WGR46_001216"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WGR46_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0856161.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WGR46_001217"
     CDS             4530..4883
                     /locus_tag="WGR46_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0856162.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WGR46_001218"
     CDS             4873..6687
                     /locus_tag="WGR46_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0856163.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLSIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WGR46_001219"
     CDS             6877..7554
                     /locus_tag="WGR46_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0856164.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WGR46_001220"
     CDS             7568..8974
                     /locus_tag="WGR46_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0856165.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WGR46_001221"
     CDS             9092..11518
                     /locus_tag="WGR46_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0856166.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNDTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WGR46_001222"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WGR46_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0856167.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WGR46_001223"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WGR46_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0856168.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WGR46_001224"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WGR46_001224"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0856169.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WGR46_001225"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WGR46_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0856170.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13483..13788
                     /locus_tag="WGR46_001226"
     CDS             13483..13788
                     /locus_tag="WGR46_001226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856171.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPRSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHIIITALPLSLSKPQIIMTPSLLLSGLTFRLVLALIAVSLLWGVYFWAVSA"
     gene            13785..14510
                     /locus_tag="WGR46_001227"
     CDS             13785..14510
                     /locus_tag="WGR46_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0856172.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14534..15409
                     /locus_tag="WGR46_001228"
     CDS             14534..15409
                     /locus_tag="WGR46_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0856173.1"
                     /translation="MNLYDLLLAPFAEFDFMCYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15486..16400
                     /locus_tag="WGR46_001229"
     CDS             15486..16400
                     /locus_tag="WGR46_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0856174.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQNLVGANQDTHAYHMTSGDIKKIRSAKLVLINGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
BASE COUNT         4334 a         5288 c         3641 g         3326 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagcaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cagacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac gcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag
     2641 ggtaatctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctattagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtagag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacggcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctaccgcgtc atccaactgc ctgccggcat cgacgaatcc gttaaagccg
     4021 gcgaaacacc tttcgcttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt cccttgcttg ggaactgtgg attgcaccgt
     4621 tacgcccggg cggttcgtgg cttgcgctca aaaccctgcc cctctgcctg ccgctttcag
     4681 gcatcctgaa gaaaaaaatc tacacctacc aatacagctc catgttgatt ctgatttatt
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatccggc agaacaaatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgccc gtatccggtg cgcgccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aggcagcaac cgtcgcccga cttttaccac gatgctgcag ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cagcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc tttatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa taccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagctg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgcctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcaaccg tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag ccctcgaagc cgccgacccc gccctaatcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgtc caattccaat tcggcagcca
    10501 ctccctctat ggcgacatcc tgaccgcacg ttgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac cgtcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcacacccca aaacactgtc
    10981 cctaaaaaac gacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaatcgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcacacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccctttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa cgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatc cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacagcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcacggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt actcgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcttccc aagctgcaaa agtcatttac ctcagccccc
    12601 aaggaaaacc gctgacacac ccaaaagcgg cagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acgctatgag ggaatagacg aaagactgct gcaaaccagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcagtttaa
    13021 tcccaaagga atcccgcatc ttgaataaaa tcctacaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggtc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgcc ccgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatct acctttctta
    13921 aatccttgat gggattgcag cccatcgaca ctggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcaat ccgacatcga ccgctcgcag cctatgaccg
    14041 ttttcgactt ggcggcaatg gggctgtggt atgaaatcgg ttttttcaga ggaataaatg
    14101 ccgttcaaaa acaacgcgtt cacgaatctt tggaacgcgt cgggatgcgg cagtttgccg
    14161 gccgccaaat tgcccacctt tccaacggac aattccagcg tgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctgg acgaaccttt caacgctgtt gatgcacgga
    14281 caacctacga gcttctcgac gtgttgcaaa aatgccattg cggcggacac gctatcattg
    14341 ccgttttgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcgtcataa atgccgtctg
    14521 aaaccgaaaa accatgaacc tctacgacct gctccttgcc ccctttgcag aattcgactt
    14581 catgtgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac ccgtcggcgt
    14641 attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg ccgtcttgcc
    14701 cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg gtttgggcgg
    14761 cgtagccgca ggcatgctga tggcactgct tgccggactt gttagccgct tcaccaccct
    14821 gaaagaagat gccaactttg ccgcctttta tctcagcagc ctcgccatcg gtgtagtcct
    14881 cgtcagcaaa aacggcagca gcgtcgattt gctgcatctg cttttcggct ctgtacttgc
    14941 cgtcgatatt cctgccctgc aactcatcgc cgccgtatcc accctcacac tgcttaccct
    15001 tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgttcc tcaaatccgt
    15061 cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca tgaacctcgt
    15121 atccggcttt caagccctcg gaacactcat gtccgtcgga ctcatgatgc tgccagccat
    15181 taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg ttctgacagc
    15241 cctgctgtgc ggcttgagcg gactgcttat ttcctaccac atcgaaattc cctccggtcc
    15301 cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg gcaaagaagg
    15361 cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac cccgcaagcc
    15421 gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat cacaggagaa
    15481 cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc gccgcaactg
    15541 ccgcccccct tccggttgta accagtttca gcattttggg cgacgtagcc aaacaaatcg
    15601 gcggagagcg cgtatccata caaaatttag tcggcgccaa ccaagatacg cacgcctatc
    15661 atatgaccag cggcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg attaacggct
    15721 tgggacttga agctgccgat gtgcaacgcg ccgtcaaaca aagcaaagta tcctataccg
    15781 aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac catcacgacc
    15841 acgatcatga ccacgaagga caccaccacg accacggcga atatgacccc cacgtctgga
    15901 acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg ataaaggccg
    15961 accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg cagctcaaaa
    16021 aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa cgcaaagtcc
    16081 tgaccgggca cgatgccttt tcctatatgg gcaaacgtta ccatatcgaa ttcatcgccc
    16141 cgcaaggcgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc atcatccgac
    16201 aaatcaaacg cgaaggcatc aaagccgtat ttaccgaaaa tatcaaagac acccgcatgg
    16261 ttgaccgcat cgccaaagaa accggcgtca acgtcagcgg caaactgtat tccgacgcac
    16321 tcggcaacgc gcccgcagac acctacatcg gcatgtaccg ccacaacgtc aaagccttga
    16381 ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg caaaaggccg
    16441 tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacaaagccg cagacagtac
    16501 agatagtacg gtaaggcgag gcaacgctgt actggttttt gtgaatccac tatattgcta
    16561 gaagtgcacc gattaaggtt aattaaaat
//
LOCUS       DAWFKN010000039        16208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFKN010000039 DAWFKN010000000
VERSION     DAWFKN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.039"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGR46_001230"
     CDS             <1..69
                     /locus_tag="WGR46_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0856175.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            76..151
                     /locus_tag="WGR46_001231"
     tRNA            76..151
                     /locus_tag="WGR46_001231"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Trp,seq:cca)
     gene            266..544
                     /gene="secE"
                     /locus_tag="WGR46_001232"
     CDS             266..544
                     /gene="secE"
                     /locus_tag="WGR46_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0856176.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            546..1082
                     /gene="nusG"
                     /locus_tag="WGR46_001233"
     CDS             546..1082
                     /gene="nusG"
                     /locus_tag="WGR46_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0856177.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1183..1617
                     /gene="rplK"
                     /locus_tag="WGR46_001234"
     CDS             1183..1617
                     /gene="rplK"
                     /locus_tag="WGR46_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0856178.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1617..2312
                     /gene="rplA"
                     /locus_tag="WGR46_001235"
     CDS             1617..2312
                     /gene="rplA"
                     /locus_tag="WGR46_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0856179.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2542..3042
                     /gene="rplJ"
                     /locus_tag="WGR46_001236"
     CDS             2542..3042
                     /gene="rplJ"
                     /locus_tag="WGR46_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0856180.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3100..3471
                     /gene="rplL"
                     /locus_tag="WGR46_001237"
     CDS             3100..3471
                     /gene="rplL"
                     /locus_tag="WGR46_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0856181.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3662..7840
                     /gene="rpoB"
                     /locus_tag="WGR46_001238"
     CDS             3662..7840
                     /gene="rpoB"
                     /locus_tag="WGR46_001238"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0856182.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7992..12167
                     /gene="rpoC"
                     /locus_tag="WGR46_001239"
     CDS             7992..12167
                     /gene="rpoC"
                     /locus_tag="WGR46_001239"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0856183.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12458..12715
                     /locus_tag="WGR46_001240"
     CDS             12458..12715
                     /locus_tag="WGR46_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856184.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12712..12861
                     /locus_tag="WGR46_001241"
     CDS             12712..12861
                     /locus_tag="WGR46_001241"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0856185.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            13040..13411
                     /gene="rpsL"
                     /locus_tag="WGR46_001242"
     CDS             13040..13411
                     /gene="rpsL"
                     /locus_tag="WGR46_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0856186.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13529..13999
                     /gene="rpsG"
                     /locus_tag="WGR46_001243"
     CDS             13529..13999
                     /gene="rpsG"
                     /locus_tag="WGR46_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0856187.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14018..16123
                     /gene="fusA"
                     /locus_tag="WGR46_001244"
     CDS             14018..16123
                     /gene="fusA"
                     /locus_tag="WGR46_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0856188.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4298 a         3599 c         4204 g         4107 t
ORIGIN      
        1 ctgcgctttg cgattcgcga aggcggtcgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaag tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg
      121 gttgggggtt cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt
      181 taattttttt gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag
      241 acagatgcta tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa
      301 agtggatcaa ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt
      361 ttttgcatat ttctcaaatt cttggttcga attcaaaaag gtggtttggc ctaagcgtga
      421 agatgctgtc agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat
      481 ctatgcggca gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg
      541 ttgagatgtc gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc
      601 aacgaatatt ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc
      661 tggtgcctgt agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa
      721 agtcatatcc tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg
      781 taaaaagcac cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga
      841 ttagtcagag agaggctgaa attattttac agcaggttca gaccggcata gagaagccga
      901 aaccaaaagt tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg
      961 attttaacgg ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc
     1021 agatatttgg tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact
     1081 gatttttata cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc
     1141 ggaaatggtt ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc
     1201 ggctatatta aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct
     1261 gctttgggtc agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc
     1321 caaggtatgg agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca
     1381 ttcacatttg tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg
     1441 caaaaaggta gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg
     1501 gaagaaattg ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc
     1561 cgtactatag caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg
     1621 ctaaagtatc taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg
     1681 caattgatga agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg
     1741 ttgacgtatc tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt
     1801 cagtcgttct gcctaaaggc accggtaaga taactcgcgt agctgtattt actcaaggtg
     1861 caaatgcaga agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg
     1921 ctgaaatcaa agcaggcaat ctgaactttg atgtcgttat tgcttctccc gatgcaatgc
     1981 gtattgttgg tcagttgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag
     2041 taggtactgt tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtac
     2101 aataccgtac agataaagca ggtatcgttc atgcaacgat tggtcgtgct tctttcgctg
     2161 aagctgactt gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg
     2221 ctgccgctaa aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta
     2281 ttcgcgttga tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt
     2341 ttctgggctg cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat
     2401 cgtaagattt aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt
     2461 gcttgtaaga tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt
     2521 ataaacagtg ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag
     2581 gaaattagcg cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc
     2641 agtgtttcca gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc
     2701 gttctgaaaa atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc
     2761 gatcaaatgg ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa
     2821 gtgttgcacc aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat
     2881 ggcgaagtaa tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag
     2941 ctgttgtcca aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg
     3001 gctgctttgg cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta
     3061 atcaattatt ttttaataca atatttggag taaaatagca tggctattac taaagaagac
     3121 attttggaag cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt
     3181 gaagaaaaat tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt
     3241 gctgccgatg ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggtgatcaa
     3301 aaagtcggcg tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa
     3361 gacatcgttg acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa
     3421 gacatccaaa aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct
     3481 tcttatgaag gctggcagtt ttctgccagc cttattttgc ttcttaaaat aagcattaag
     3541 tattgtttac atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca
     3601 gattgatgcg tgccgttgct ttcagacggc ctattattga aaattacttt tcggagtgta
     3661 tatgagctat tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa
     3721 tgttttggaa gttcctttct tgctggcaac ccaaattgat tcttatgcga agtttttgca
     3781 gctggaaaat gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat
     3841 tttcccgatt gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg
     3901 cgagcctttg ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt
     3961 gcgcgcgcgt atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga
     4021 agttcgtgaa aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt
     4081 tgtgattaac ggcacagagc gtgtgattgt ttcccagttg caccgttcgc ccggcgtgtt
     4141 tttcgagcat gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat
     4201 cattccctac cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt
     4261 ccgtatcgac cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa
     4321 caatgagcaa atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg
     4381 tgttcaaacc gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt
     4441 ggataaagaa ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg
     4501 tgatattacc aatgcaggcc tgacccgttt ggatgtagaa ccagaaagcc tgctgggcaa
     4561 agcattggct gccgacctga ttgattcgga aaccggcgag gtattggctt ctgccaatga
     4621 tgaaattaca gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac
     4681 cctttacatc aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga
     4741 gactgccggc cggcaggcgg cgcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc
     4801 gcccactgaa gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta
     4861 cgatttgtcc cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc
     4921 cgaagcccaa caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc
     4981 cgacaaaggc ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt
     5041 cgagttgcgt aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt
     5101 acgttcggta ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg
     5161 tgccgtaaaa gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat
     5221 taatgcaaaa cctgtttctg ctgctattaa agaattcttc ggctccagcc aattgagtca
     5281 gtttatggat cagaccaatc ccttgtctga agtaacccat aaacgccgcg tatccgcatt
     5341 gggtccgggc ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac
     5401 ccactacggc cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa
     5461 ctcactgtcc gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg
     5521 cgttatcgac ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg
     5581 ctatgtgatt gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt
     5641 ggttacctgt cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat
     5701 ggacgtggca accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca
     5761 tgatgacgcg aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt
     5821 gcgtcctgaa aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc
     5881 tactgcaatc gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgcgtcgt
     5941 cgttcgtgtt catgacgatg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt
     6001 ggttaaattt acccgttcca atcaatctac taacatcaac caacgtccgg ctgtcaaagc
     6061 aggcgatgtt ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga
     6121 attggctttg ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga
     6181 agactcgatt ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat
     6241 tgaggaattg aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga
     6301 tattccgaac ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat
     6361 cggtgcggaa gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac
     6421 ccagctgacg ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt
     6481 aaaagatact tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt
     6541 cttcactcgt gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgagtt
     6601 gaaacgctac cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga
     6661 ccgtatcgag cgtatgattg tcggtcagaa agccaacggc ggcccgatga agctggccaa
     6721 aggcagtgaa atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga
     6781 tatccgtctg accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca
     6841 acaaaaacgc gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg
     6901 cgacgaattg caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg
     6961 tctgcaagcc ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat
     7021 tctgccagtg gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa
     7081 cccattgggc gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg
     7141 ggcagcaaaa ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg
     7201 cgagttgcgc gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga
     7261 tgccctgact gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt
     7321 cgcctctcct gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgc tgaacttggc
     7381 ttatccgagc gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat
     7441 cacgctgtat gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat
     7501 gcactatctg aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc
     7561 gtacagtctg gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt
     7621 cggcgagatg gaggtttggg cattggaagc atacggcgcg gcatacacgc tgcaagagat
     7681 gttgactgtg aagtctgacg acgtgaacgg tcgtaccaaa atgtacgaaa acatcgtcaa
     7741 aggcgaacac aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat
     7801 tcgctcactg ggcttggata tcgatttgga acgctactga tacgggtttc agacggcata
     7861 aggggagctg ttctgcaggt atgcggggca gccgacaatg tttaaaaacg aaatgccgtc
     7921 tgaaaacact gtacctctat ccatatcgaa aatccgccat gcggtaaaaa tacttccttc
     7981 aaggagcaaa aatgaatttg ttgaacttat ttaatccgtt gcaaactgcc ggcatggaag
     8041 aagagtttga tgccatcaaa atcggtattg cctctcccga aaccatccgc tcatggtctt
     8101 atggcgaagt caaaaaacct gaaaccatca actaccgtac gttcaaacct gagcgtgacg
     8161 gtttgttctg tgccaaaatc tttggcccgg tcaaagacta cgaatgcttg tgcggaaaat
     8221 acaaacgctt gaaatttaaa ggtgtaactt gtgaaaaatg cggcgtggaa gtaaccctgt
     8281 ccaaagtgcg ccgcgaacgc atgggccata tcgaattggc tgcgcccgtc gcacatattt
     8341 ggttcttaaa atccctgcct tcccgcttgg gtatggtgtt agacatgact ttgcgcgata
     8401 tcgaacgcgt attgtacttt gaagcatttg tggtaaccga tcccggtatg actccgctgc
     8461 aacgccgcca attgctgact gaagacgatt actacaacaa gctggacgaa tacggcgacg
     8521 atttcgatgc caaaatgggt gcggaaggta tccgcgaatt gctgcgtacc ctgaatgtag
     8581 cgggcgaaat cgaaatcctg cgccaagagt tggaatcgac cggttccgac accaaaatca
     8641 aaaaaatcgc caaacgcttg aaagtattgg aagctttcca tcgttccggt atgaaactgg
     8701 aatggatgat tatggatgtg ctgccggtat tgccgcctga tttgcgtccg ttggttccat
     8761 tggatggtgg tcgttttgcc acttccgatt tgaacgattt gtaccgccgc gttattaacc
     8821 gtaacaaccg tctgaaacgt ctgttggaac tgcatgctcc tgacatcatc gtccgcaacg
     8881 aaaaacgtat gttgcaagaa gcagttgact cgctgttgga taacggccgt cgcggtaaag
     8941 ccatgaccgg cgccaacaaa cgcccgctga aatcattggc agacatgatt aaaggtaagg
     9001 gtggccgctt ccgtcaaaac ctgctgggca aacgtgtgga ctactccggc cgttccgtga
     9061 ttaccgtagg cccgtacctg cgtctgcacc aatgcggttt gccgaaaaaa atggctttgg
     9121 aactgttcaa accgttcatt ttccacaaat tggaaaaaca aggtttggcc tctaccgtta
     9181 aagcagcgaa aaaattggta gagcaagaag taccggaagt atgggacatc ttggaagaag
     9241 tcatccgcga acatccgatt atgctgaacc gtgcgccgac cctgcaccgt ttgggtattc
     9301 aagcgttcga acctatcttg attgaaggta aagcgattca gttgcaccca ttggtgtgtg
     9361 ctgcgttcaa cgccgacttt gacggcgacc aaatggcggt acacgttcca ttgagcttgg
     9421 aagcacaaat ggaagcacgc acgctgatgc tggcttcaaa caacgtattg tctcctgcca
     9481 acggcgaacc gattatcgta ccttcccaag acatcgtatt gggcctgtac tacatgaccc
     9541 gcgaccgtat caatgccaaa ggcgagggca gcctgtttgc cgatgtgaaa gaagtgcatc
     9601 gcgcatacca taccaaacag gttgaactgg gtacgaaaat taccgtacgt ctgcgcgaat
     9661 gggtgaaaaa cgaagcaggt gagtttgagc ctgtcgttaa ccgttacgaa acaaccgtcg
     9721 gccgtgcatt gttgagcgaa atcctgccga aaggcctgcc gtttgaatat gtcaacaaag
     9781 cgttgaagaa aaaagaaatt tctaaactga ttaacgcatc gttccgcctg tgcggcttgc
     9841 gcgatacggt tatctttgct gaccacctga tgtacaccgg tttcggattt gcggcaaaag
     9901 gcggtatttc cattgccgtt gacgatatgg aaattccaaa agaaaaagcg gccttgctgg
     9961 ctgaagccaa tgccgaggtt aaagaaatcg aagaccaata ccgtcaaggt ttggttacca
    10021 acggcgaacg ctacaacaag gtggtcgata tttggggtcg tgccggcgat aagattgcta
    10081 aagcgatgat ggacaacttg tccaaacaaa aagttatcga ccgtgacggc aacgaagtcg
    10141 atcaagagtc attcaactcc atttatatga tggcggactc cggtgcccgt ggttctgcag
    10201 ctcagattaa acagttgtcc ggtatgcgtg gcttgatggc aaaacctgac ggctcgatta
    10261 ttgaaacgcc gattacctca aacttccgtg aaggtctgac cgtattgcaa tactttattg
    10321 cgacccacgg tgcgcgtaag ggtttggcgg ataccgcatt gaaaaccgcg aactccggtt
    10381 acctgactcg tcgtctggta gacgtaactc aagatttggt cgttgttgaa gacgattgcg
    10441 gtacttcaga cggctttgtc atgaaggcag tggtacaagg cggtgatgtg attgaagcat
    10501 tgcgcgatcg tattttgggt cgtgttaccg cgtctgacgt tgtcgatccg tcaagtggcg
    10561 aaaccttggt tgaagccggt acgttgctga ctgaaaaact ggtggatatg atcgaccaat
    10621 ccggtgtcga tgaagtcaaa gtccgtacgc cgattacttg taaaacccgt cacggcctgt
    10681 gtgcacactg ttacggtcgt gacttggcac gcggcaaact ggttaacgcc ggtgaggcag
    10741 tcggtgtgat tgctgcacaa tccattggcg aaccgggtac ccagttgacc atgcgtacgt
    10801 tccacatcgg tggtgcggca tcccgtgcgg cagcagccag ccaagtggaa gccaaatcca
    10861 acggtacggc acgattcagc agccagatgc gctacgttgc caacaacaaa ggcgagttgg
    10921 ttgtcatcgg ccgttcttgt gaagtcgtga ttcacgacga tatcggccgt gaacgcgaac
    10981 gccacaaagt accttacggt gccatcctgc tggtacaaga cggtatggcc attaaagccg
    11041 gtcaaacctt ggcaacctgg gatccgcata cccgtccgat gattaccgaa cacgcaggta
    11101 tggtgaaatt cgaaaacgtg gaagagggcg ttaccgttgc caaacaaacc gatgatgtaa
    11161 ccggtttgtc cactttggtg gtgattgacg gtaaacgtcg ttcctctagt gcttccaaac
    11221 tgctgcgtcc gactgtgaaa ctcttggacg aaaacggcgt ggaaatctgt attcccggta
    11281 cgtccactcc ggtttcgatg gcattccccg ttggtgcggt gattaccgta cgcgaaggtc
    11341 aggaaatcgg taaaggcgac gtattggcgc gtatcccgca agcctcttcc aaaacccgcg
    11401 acattaccgg tggtctgccg cgcgttgccg aattgtttga agcacgcgtg ccgaaagatg
    11461 ccggtatgtt ggcggaaatt accggtaccg tttccttcgg caaagagacc aaaggcaagc
    11521 aacgtctgat tgttactgac gtggacggtg tagcatacga gaccttgatt tccaaagaga
    11581 aacaaattct ggtacacgac ggtcaagtgg taaaccgcgg tgaaaccatc gtggacggag
    11641 ctgttgatcc gcatgatatt ctgcgtttgc aaggtatcga agcactggca cgctacattg
    11701 tccaagaggt gcaagaggtt taccgtctgc aaggtgtgaa gatttctgat aaacacatcg
    11761 aagtcatcat ccgtcaaatg ttgcgccgtg tgaacattgc ggatgccggc gaaaccgggt
    11821 tcattaccgg agagcaggtc gaacgcggcg atgtgatggc ggccaatgaa aaagctttgg
    11881 aagaaggcaa agagccggca cgttacgaaa acgtattgct gggtattacc aaagcctccc
    11941 tgtccaccga cagtttcatt tctgccgcat cgttccaaga aacgacccgt gttctgaccg
    12001 aagccgcgat tatgggcaaa caagacgaac tgcgcggttt aaaagagaac gtcatcgtcg
    12061 gccgcctgat tcctgccggt accggtttga cttatcaccg cagccgtcat cagcaatggc
    12121 aaggggtaga gcaggagact gctgaaaccc aagtaacgga tgaataatct tcggcgcgtt
    12181 tattcaatac aaaaccgcaa gctttaagct tgcggttttt ctttgtccga ttaaggcaaa
    12241 aacaagcgtt ttcgtcattt tgaggcgtgt ggattattcc ttaggtattt tcgggccgga
    12301 gaccaacgag gtggcgggtg tcgtcggtac gtccggagac caaaataact ttgccaagga
    12361 tgttggtttc ggcggtcaaa aaagtagcgt cttaatgttt tccatttaaa caaatgtcgt
    12421 ctgaaacttc agacggcatt tcctttaaaa aataaatatg aaacccagaa atctcttttt
    12481 tgcaggctgc ctgctgactt cggcgacgtt tgccgaggat atcggcgtac ctgtcgaact
    12541 gattaacgtc ggtaatcgga ttgcgatgcc gtctgaaggg gaaagcctcg ccctcctgcc
    12601 gtttgccgag gatgtaccgc cggttcgcga tgcaatgccg tctgaagttc ctaaaagcgc
    12661 ggcaggcggc gatgttcggg gtgaccggat gggaatgccg attaacatcg gatgagcgcg
    12721 gctttatggc ataaaaaact gtcgtggaaa ggatttacac cccaaataaa tttccgttac
    12781 aacaagatca acagcaatat gcccgccttt tattcgcgca gcagcaagga atggtttgtc
    12841 agtatagaaa aaacgtattg acagtatttt ctccagccgt ccgactgatt gtgagggatg
    12901 tcggtaaata tttatcggca aacaagaaaa tcatctttct tcttgtcgtt atgcttgact
    12961 gtatacttgc aataaaaata taattccact cttgccgaca tggtgtcggc aagtatttaa
    13021 ctcaacagga cgagaaaata tgccaactat caaccaattg gtacgcaaag gccgtcaaaa
    13081 gcccgtgtac gtaaacaaag tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg
    13141 cacccgtgta tacacgacta cccctaaaaa acctaactct gcattgcgta aagtatgtaa
    13201 agtccgcctg accaacggtt ttgaagtcat ttcatacatc ggcggcgaag gccacaacct
    13261 gcaagagcac agtgtcgtat tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg
    13321 ttaccacact gtacgcggtt ctttggatac tgcaggtgtt aaagaccgta aacaagcccg
    13381 ttccaaatac ggtgctaagc gtcctaaata attactggga cttaaatagg cacgtcggcc
    13441 gcctaagctg aacaacggcc gagtaagtga atactcaatt gggtattcat gggaatagac
    13501 ccgactgaat agattaaagg aaattaaaat gccaagacgt agagaagtcc ccaagcgcga
    13561 cgtactgcca gatcctaaat tcggcagcgt cgagttgacc aaattcatga acgtattgat
    13621 gattgacggt aaaaaatccg ttgccgagcg tatcgtttac ggtgcgttgg aacagattga
    13681 gaaaaaaacc ggcaaagtag caatcgaagt atttaacgaa gccattgcaa acgccaaacc
    13741 tatcgtggaa gtgaaaagcc gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt
    13801 tcgtccttca cgccgtttgg ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg
    13861 tggtgagaaa tccatggacc tgcgtttggc aggcgaattg attgatgcgt ccgaaggccg
    13921 tggcggtgcg ttgaaaaaac gtgaagaagt acaccgtatg gctgaagcca acaaagcatt
    13981 ctctcacttc cgtttctaat tttgaaaggc taataaaatg gctcgtaaga ccccgatcag
    14041 cctgtaccgt aacatcggta tttccgccca tatcgatgcg ggtaaaacca cgacgacaga
    14101 acgtattttg ttctataccg gtttgaccca caagctgggc gaagtgcatg acggtgcggc
    14161 tactaccgac tacatggaac aagagcaaga gcgtggtatt accattacct ctgctgccgt
    14221 tacttcctac tggtccggta tggcgaaaca attccccgag caccgcttca acatcatcga
    14281 caccccggga cacgttgact ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg
    14341 cgcggtaatg gtttactgcg cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg
    14401 gcaagccaac aaataccaag tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg
    14461 tgccaacttc ttccgcgttg tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc
    14521 tatcgtcatt ccggttggtg cggaagacaa cttcagcggt gtggttgatt tgttgaaaat
    14581 gaaatccatc atttggaatg aagtcgataa aggtacaacc tttacctatg gcgatattcc
    14641 tgccgaattg gtcgaaactg ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga
    14701 agccagcgaa gaactgatgg acaaatactt aggcggcgac gagctgaccg aagaagaaat
    14761 cgtaggcgcg ttgcgtcaac gtactttggc aggcgaaatt cagcctatgc tgtgtggttc
    14821 tgcatttaaa aacaaaggtg ttcaacgtat gttggacgca gttgtagaat tgctgccagc
    14881 tcctaccgat attcctccgg ttcaaggtgt caacccgaat accgaggaag ccgacagccg
    14941 tcaagccagc gatgaagaga aattctctgc attggcgttc aaaatgttga acgacaaata
    15001 cgtcggtcag ctgaccttta tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt
    15061 attgaactcc gtaaaaggca ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc
    15121 agaccgtact gaaatcgaag aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa
    15181 agacgttact accggtgaaa ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat
    15241 ggaattcccc gagccggtaa tccatattgc cgttgagccg aaaaccaaag ccgaccaaga
    15301 gaaaatgggt atcgccctga accgcttggc taaagaagac ccttctttcc gtgtccgtac
    15361 agacgaagaa tccggtcaaa ccattatttc cggtatgggt gagctgcact tggaaattat
    15421 tgttgaccgt atgaaacgcg aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc
    15481 ttaccgtgaa actatccgca aagccgttaa agctgaatac aaacatgcaa aacaatccgg
    15541 tggtaaaggt caatacggtc acgttgtgat tgaaatggaa cctatggaac cgggtggtga
    15601 aggttacgag tttatcgatg aaattaaagg tggtgtgatt cctcgcgaat ttattccgtc
    15661 tgtcgataaa ggtatccgcg atacgttgcc taacggtatc gttgccggct atcctgtagt
    15721 tgacgtacgt atccgtctgg tattcggttc ttaccatgat gtcgactctt cccaattggc
    15781 atttgaattg gctgcttctc aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct
    15841 gcttgagcca atcatggcag ttgaagtgga aaccccggaa gaatacatgg gcgacgtaat
    15901 gggcgacttg aaccgccgtc gcggtgttgt attgggtatg gatgatgacg gtatcggcgg
    15961 taaaaaagtc cgtgccgaag tacctctggc agaaatgttc ggttattcga ccgacctgcg
    16021 ttctgcaacc caaggccgcg ctacttactc tatggagttc aagaaatatt ctgaagctcc
    16081 tgcccacata gctgctgctg taactgaagc ccgtaaaggc taatcaggca aataggccgt
    16141 ccgaaaggct gaaatgattt ttcagacggc attgttcttt aatcgatctt taatgtaaag
    16201 gaattagc
//
LOCUS       DAWFKN010000040        16082 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFKN010000040 DAWFKN010000000
VERSION     DAWFKN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.040"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(309..731)
                     /locus_tag="WGR46_001245"
     CDS             complement(309..731)
                     /locus_tag="WGR46_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0856189.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(849..1520)
                     /locus_tag="WGR46_001246"
     CDS             complement(849..1520)
                     /locus_tag="WGR46_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0856190.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1536..2162)
                     /locus_tag="WGR46_001247"
     CDS             complement(1536..2162)
                     /locus_tag="WGR46_001247"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0856191.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDRLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2269..3087)
                     /locus_tag="WGR46_001248"
     CDS             complement(2269..3087)
                     /locus_tag="WGR46_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0856192.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGTVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3346..3786)
                     /locus_tag="WGR46_001249"
     CDS             complement(3346..3786)
                     /locus_tag="WGR46_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0856193.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSETSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3849..5255)
                     /locus_tag="WGR46_001250"
     CDS             complement(3849..5255)
                     /locus_tag="WGR46_001250"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0856194.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5425..6540
                     /gene="lptF"
                     /locus_tag="WGR46_001251"
     CDS             5425..6540
                     /gene="lptF"
                     /locus_tag="WGR46_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0856195.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6537..7607
                     /gene="lptG"
                     /locus_tag="WGR46_001252"
     CDS             6537..7607
                     /gene="lptG"
                     /locus_tag="WGR46_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0856196.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGLIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7917..10502
                     /gene="acnB"
                     /locus_tag="WGR46_001253"
     CDS             7917..10502
                     /gene="acnB"
                     /locus_tag="WGR46_001253"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0856197.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10644..11639
                     /locus_tag="WGR46_001254"
     CDS             10644..11639
                     /locus_tag="WGR46_001254"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0856198.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11708..11809)
                     /locus_tag="WGR46_001255"
                     /pseudo
     CDS             complement(11708..11809)
                     /locus_tag="WGR46_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11863..12876)
                     /gene="ilvC"
                     /locus_tag="WGR46_001256"
     CDS             complement(11863..12876)
                     /gene="ilvC"
                     /locus_tag="WGR46_001256"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0856199.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12956..13249)
                     /locus_tag="WGR46_001257"
     CDS             complement(12956..13249)
                     /locus_tag="WGR46_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0856200.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(13316..13807)
                     /gene="ilvN"
                     /locus_tag="WGR46_001258"
     CDS             complement(13316..13807)
                     /gene="ilvN"
                     /locus_tag="WGR46_001258"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0856201.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13818..15545)
                     /gene="ilvB"
                     /locus_tag="WGR46_001259"
     CDS             complement(13818..15545)
                     /gene="ilvB"
                     /locus_tag="WGR46_001259"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0856202.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         3763 a         4371 c         4093 g         3855 t
ORIGIN      
        1 caataaatta atatatggct taaaataacg ggatgcccgc cttccgcccg cccgcagaag
       61 caggcgggta tcattttaaa acgcggcatt tgaaatttga ccgaaaattg ttgacaatcc
      121 ggaatcaagc ctgcacaata ccccgacaag tccaagtatt ataaaggctg aataaagagg
      181 aaacagcagg cagatatatt cgggaggtgc agtccgaata tatctgcttt tttatgcgcc
      241 tccggattgc ctgccgcacc tttcccttca gacggcatca gccgtttccc cataatgccg
      301 cccgatgcct atttatctgc cccggcaatt tcaaaactgt gggtaatctt tgccgctttg
      361 cccaacataa tcgaagccga acagtatttt tcggcagaca tctgaacggc gcgctcaatg
      421 gccgattctt tcaaatcatg cccgaatact ttgaaatgga tgtggatttc ggtaaacacg
      481 cgcggcgcat cgtccgcccg tttcgccgta accgtcgcac ggcagtcagt cactttctga
      541 cgctgttttt cggcaatcat caccacatcg atgctcgaac agcccgccac gcccaacagc
      601 agcatttcca aagggctggg cccgcgctta gccttacctt ctgccgccga cccctccata
      661 acgacgctgt gcccgccttc cgttgttccg acaaagcaca ttccgtctat ccattttgat
      721 gtaacctgca tggtgtcatt cctgaaaata gcgttaaaac cgctttgcat atggcgttat
      781 tgtaaacaat ttcaagcggc ttatgcagaa atatggacaa aacggcaaaa aaacgcctga
      841 aaatcgattt acggtttggc ggactggcca ttgatctgca ccgatttgag tttcagcgta
      901 taggttttgc cgtcgtcggt atagccgatt tgtgccggaa tattgttcag ggacggtgcg
      961 aagaaataca ttaccgtatc gtcgccgcgc cgcacccgat atttgacgac ttcggtttcc
     1021 acgccgccta tgctgtattt tcctgtaccc gccttattca aaccgccgac ggaataaagt
     1081 tttttgccgt tggtgatttt cagccccggg gggagtttcg cgtcatttgc cgccaactgc
     1141 caggcaagcg tgaacaaatc catagccttg gggctttgct cggttttgct ctcgcccgct
     1201 ttgccgtagg ttacgctgcc gtcggcgaat ttggcttccg catacagttt gcccctgcgt
     1261 atgtctctat agtaggtagg gtgcagggta ttgccgacaa ccgtaccgcc ggactcgaaa
     1321 cggatattgt atagcggcac tttaatcgtc gaaacgattt tgtaagcatt gccgctgcgt
     1381 tcaaatgtca tcgtggcggg aatgccgtag ctgccggaat agtgcagcac ggcggattgg
     1441 ggcagccctg ccgcatacgc gcacggcagg gcggcggaca aaatggcggc ggaaaatata
     1501 tttttaaaag tcttcatcat ttgctcccgc ccggtttacg ccgtcagaaa acgggcggca
     1561 tcggcgtttt ccgaatttct gacgcggttt ccctcaataa tcaggcggcc ggcggcaaaa
     1621 tcggcaacgg ctttcggata aagtttatgc tcgacagcca aaacccgggc ggcaacatcg
     1681 tctgccgtat cgccgtcgag tatcggcaca atcccttgcg atacaatcgg gccgcaatcc
     1741 agttcggcag taacgaaatg gatggtacag ccggcaacgc ggcaacctgc ctccaaagcg
     1801 cgttcgtgcg tatgaagtcc ggtaaacgag ggaaggatgg acgggtggat gttcatcagc
     1861 ctgccttcgt aatgggcgca aaactcgggg gtcagaatcc gcataaaacc tgccaaaacc
     1921 accaagtcgg gttgatatgc gtcgattttt tccatcatgg cggtatcgaa ggcaagccgg
     1981 gatgtaaagt ttttatgatt cagtctatcg gtcgggatgc cgcgttcggc cgcccattgc
     2041 aaaccggcag ccgtttcgct gttgctcaac acggcggcaa tgcggacgtt gggaatagcg
     2101 gcattgacga ttgcctgcat attgctgccg cgcccggaaa tcaggatgac gatgtttttc
     2161 ataatggtgc gcttttgaaa gggatgccgt ctgaaccgct gtttggtggt ttcagacggc
     2221 atttgccgta aaaatgcccg aaaacctgtt tcgggcatgg attcggactt aatttacttt
     2281 tttgatgtcg acttgagccg gctgcttggc gggcgcgttt tcgggtgcgc cgattttgac
     2341 cagtttcaca tcaaatacca aagtggcgtt cgggccgatt ttgtcgcccg caccctgttc
     2401 gcggtaggca aggttggacg ggatgtagaa cgtggcttcg ccgccttctt tcagaagttg
     2461 tacgccttcg gtccaacccg gaatcacttg gctcaaaggg aaggtgaccg tgccgccgtt
     2521 ggctttgctg ctgtcgaata ccgtaccgtc aatcaggcgg ccttcgtatt ccacggtaac
     2581 gatgtcgtct ttggtcggct gtttgccttc accctgtttg gtgattttgt attgcaggcc
     2641 ggaagcagtg gtcttcacgc cgtctttggc ggcattttct ttcagaaagg cttcgccttt
     2701 ttctttattg gccttcgcgt ccgccttgtg tttttctacg gctttagcct gttgttcctg
     2761 aaggaatttc atcatgactt cctgagcctg ctcttcggtc attttgattt ctttgccgtc
     2821 atacactgcc tgcatggctt cggtaaagac tttcaaatcg atttccgcgc cctgttcctt
     2881 catttgcttc agggagcgtc cgatgtccac gcccatcgca tagcttgcct gctgcatcgt
     2941 gctgccgatc gaagaggtgt cgccctgcgc ggaagaagcg gcggcaggtt cggatgcaga
     3001 tgcgggggcg gcttcttttt tgccgcaggc ggaaagtgcc aaagcggcgg aaagggtcag
     3061 tgcgctgatt ttgaaaatgg tgttcatgat ggatcttcgc tgtcgataag gtcggaaaaa
     3121 cgggattata gccgagtttg aatgtttcaa cacacaggat gacacataaa gcgtcaatcg
     3181 tgtgttgccc tgttttggaa gggattgaac cttccaaaat aagttttgat tctaccgccc
     3241 cgagggacag atgtccaagt ggcggggttc aaccgataag gaaattttaa tcaaatagaa
     3301 tcaagcctgt ttaaattttg taaatgcggc atttcagacg gcattttatg ccttgccctc
     3361 catgccgtga tgttcgatgg caaaaccgct tcggcggtag gcggtaaagc gttcgcgcgc
     3421 gtcggcgaga tcttccaagc tgttgccgac gatttccaaa acgcgcgcgg gcaagaccgg
     3481 agcggtgttc caaaaaccgt cggacaggtt caaaacggtc atgccttcgg gaattcgggg
     3541 cagattgccg ccgcaggcaa gcaggacgga tgtttcagac ggcatggctt cttccgtttc
     3601 ccaaatttcg tgcggaatga aactctcggc ctcgtattgc caaagcattt tgtccaattc
     3661 ctgaagctgc ccgaacgaat cggaccacac cagtatcctg ccgccgtccc gaatcgcacg
     3721 ggcaatcagg cggcaggtga aaatcggaac ttgggcaacg tgcgtgtaaa aggtggcttt
     3781 cggcatattg tttgaacatt tggcaggata atgccgtctg aaaggcttca gacggcattg
     3841 tggcaaaatt aaagattccg cagatagttc agcagcaagg gaacgggacg gccggtcgca
     3901 cctttttccg caccggattt ccacgccgta cccgcgatgt caaggtgcgc ccacgggtag
     3961 ccttcggtaa agtaagacag gaaggtggcg gcggtaatcg tgcccgcgcc gggcgtgccg
     4021 atattgggga tgtcggcaaa attggacttg agctggtctt tgtaggtttc aaagagcggc
     4081 agttgccacg ctttgtcgtc cacgttgcgg gaggcggcga gcaggctgtc gatcaaatcc
     4141 tgattgttgc ccatcacgcc gctgacatcg tgccccaagg cgacgatgca tgcaccggtc
     4201 agggtggcga cgtcgatgac ggctttgggt ttgaactgtt cggcgtaagt gagcgcgtcg
     4261 cacaaaatca gacggccttc ggcatcggtg ttcaacactt cgatggtcag ccctttcatg
     4321 ctttttacga cgtcgcccgg tttgttggcc gcgccggaag gcatgttttc acaagtggcg
     4381 acaacggcaa tcagattaat cggcagttgc agtttgacgg cggcgcagaa ggtgctgatg
     4441 acggttgccg caccgcacat gtcgaacttc atttcgtcca tgttcagacc gggtttgagg
     4501 gaaatgccgc cggtgtcgaa ggtaatgcct ttgccgacca ataccacagg cgcggcttct
     4561 ttgtccgccg caccgaaata gctcagttcg accaaatagg gatcttcgac actgcctttg
     4621 gcgaccgacc aaaacgaacc catgttttct ttgatgtagt ctttttcgat gattttggcg
     4681 tgcgcgccca gtttttcggc ttcggctttg gcggtgcgcg ctaaaaattc gggcgtgcat
     4741 tcgttgggcg cggcgttgcc caagtcgcgg cagaggcttt gtccgtaaac ttgcgcttcg
     4801 gcgacgcgca aggcttcttt aacgacggct tcgtgtgcgc tgtggaacac ggcggtttca
     4861 aatttggcgg gcttggcttc ttttttgtag cggtcgaaac ggtaggcggc gttgccgaac
     4921 gcaatcgcaa acgcttcggc tacggcggcg gcttgcgctt catcgaaggc atggatgtcc
     4981 acattgaccg tttcctgatt ttgcgcccat ttggcggctt cggcggcggc cttgttcaat
     5041 gcggcgcggc cggtgctttt cagacagcat acggcaacag cctgcaaacc gttgcctgtc
     5101 gggatttttg tgtcggcaaa attttgacct tcttcaagcg aagacaaaag ggcaagggcg
     5161 gtcggatggt ttaattgcgc ctcttcggtg cagacaaata actgtgcgcc tgcctgctgt
     5221 tcctgcaaga tttcggtttt tgtgctaaat tccacgttta ttctcctgat tgagacggtt
     5281 gtcggtagtt ttcggacggc ctttcgctca aaagaccgtc tgaagacggc tggcacgatt
     5341 gtaccccatt tgaagcaccg tctgaaacct tgcgcggaca atccgcctgc gccgaaccgc
     5401 ttaccgcccc ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct
     5461 tttaccgccg tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc
     5521 aacctgctcg gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc
     5581 ggcttctggg tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg
     5641 ttgaccgtgt tgacccgcta ctggcgcgac agcgaaatgt cggtctggct atcctgcgga
     5701 ttggcattga aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg
     5761 gttgccgtca tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct
     5821 gaaatcctga agcagaagca ggaattgtct ttggtggagg caggcgggtt caacagcttg
     5881 ggcaagcgca acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg
     5941 aaaaacctgt tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa
     6001 gaaggtaact tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt
     6061 tacagcggca cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcaacctg
     6121 attatcagca ccacgcccaa actcatcgac cccgtttccc accgccgtac cattccgacc
     6181 gcccaactga ttggcagcag caacccgcaa catcaggcgg aattgatgtg gcgcatctcg
     6241 ctgaccgtca gcgtcctcct actctgcctg cttgccgtgc cgctttccta tttcaacccg
     6301 cgcagcggac atacctacaa tatcttgatt gccatcggtt tgtttttaat ttaccaaaac
     6361 gggctgaccc tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg
     6421 ctgcctatgc acatcatcat gttcgtcatc gcaatcgtac ttctgcgcgt ccgcagcatg
     6481 cccagccagc ccttctggca ggctgttggc aaaagtctga cattgaaagg cggaaaatga
     6541 acctgatttc acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg
     6601 ccttcctcgc tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag
     6661 gcagttacgg catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct
     6721 acgaactgat gcccctcgcc gtccttatcg gcggactggt ctctctcagc cagcttgccg
     6781 ccggcagcga actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga
     6841 ttctgtcgca gttcggtttg attttcgcca ttgccaccgt cgcgctcggc gaatgggttg
     6901 cccccacgct gagccaaaaa gccgaaaaca tcaaagccgc cgctattaac ggcaaaatca
     6961 gcaccggcaa taccggcatt tggctcaaag aaaaaaacag cattatcaac gtgcgcgaaa
     7021 tgttgcccga ccataccctg ctgggcatta aaatctgggc ccgcaacgat aaaaacgaac
     7081 tggcagaggc agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga
     7141 aaaacatccg ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag
     7201 aaaactggcc gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc
     7261 aaatgtccgt cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca
     7321 cccgaatcta cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga
     7381 tggcgctcgt cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa
     7441 aactcttcgg cggcatctgt ctcggattgc tgttccacct tgccggacgg ctcttcgggt
     7501 ttaccagcca actctacggc atcccgccct tcctcgccgg cgcactacct accatagcct
     7561 tcgccttgct cgccgtttgg ctgatacgca aacaggaaaa acgctaaacc aatgccgtct
     7621 gacccccttc ggacggcatt ggtttttatt atcatattcc tgtagacaga tagtcgttcc
     7681 ctattatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactattgta
     7741 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataca ttacctgtca
     7801 taacagttcc atttttgtta aaactagtct atgatagcgg tacaaatatt gtttacaata
     7861 tttaacgcaa atcatttgca acccgacaaa agaaaaacag aaaaaggaac aaagagatgt
     7921 tagaagccta tcgtaaagcc gccgccgagc gcgccgccct cggcattccc gccctccctt
     7981 tgaacgcgca gcaaaccgcc gatttggttg agctgctgaa aagcccgccc gcaggcgaag
     8041 gcgagttctt ggtcgaactg cttgcccacc gtgttccgcc cggtgtggac gatgccgcca
     8101 aagtcaaagc ctcattcctg gctgccgttg ccgaaggcag cgcgtccagc ccgctgatct
     8161 cccccgaata tgcgaccgaa ctcttaggta cgatgctcgg cggttacaat atccacgcct
     8221 taatcgaact cttggacgac gacaaactcg cgcccattgc cgccaaaggc ttgaaacata
     8281 cgcttctgat gttcgattcc ttccacgacg ttcaggaaaa agccgaaaaa ggcaacaaat
     8341 acgcgcaaga agttttgcaa tcttgggcag atgccgaatg gtttacctca cgcgccaaag
     8401 ttcccgaaaa aatcaccgtt accgttttca aagttgacgg cgaaaccaat acagacgacc
     8461 tctcccccgc gcccgacgcg tggagtcgtc ccgatattcc gctgcacgcg ctggccatgc
     8521 tgaaaaaccc gcgcgacggc atcacgcccg acaaaccggg cgaagtcggt ccgattaaat
     8581 tgttggaaga actcaaagcc aaaggccatc cggttgctta cgtcggcgac gtggtcggta
     8641 ccggttcttc acgcaaatcc gcgaccaact ccgtcatttg gcataccggc gaagacattc
     8701 cgttcgtgcc gaacaaacgc ttcggcggcg tatgtttggg cggcaaaatc gcgccgattt
     8761 tcttcaatac ccaagaagat tccggcgcgc tgccgattga agtcgatgta tctgctctaa
     8821 aaatgggtga tgtcgtcgat atcctgcctt atgaaggcaa aatcgtgaaa aacggcgaga
     8881 ctgttgccga gtttgaattg aaatcacaag tattgctgga cgaagtgcaa gccggcggcc
     8941 gtatcaacct gattatcggc cgaggtctga ccgccaaagc gcgcgaagcc ctgaaactgc
     9001 ctgcctctac tgcattccgc ctgccgcaag cgcctgccga aagcaaagcc ggtttcacct
     9061 tggcgcaaaa aatggtcggc cgcgcctgcg gtctgcccga aggacaaggc gtgcgcccgg
     9121 gtacttactg cgaaccgcgt atgacgacgg tcggctcgca agacacgacc ggcccgatga
     9181 cccgcgacga gttgaaagac ttggcttgtt tgggcttctc cgccgatatg gtgatgcagt
     9241 ctttctgcca caccgccgcc tatccgaaac ctgtcgatgt aaaaacccat aaagaactgc
     9301 ccgcctttat ttccacccgt ggcggcgtgt cactgcgtcc gggcgacggc gtcatccact
     9361 cgtggctcaa ccgcctgctg ctgcccgata ccgtcggcac cggcggcgac agccataccc
     9421 gtttccccat cggtatttcc ttccccgccg gctccggctt ggttgccttt gccgccgcaa
     9481 cgggcgtaat gccgctcgat atgcccgagt ctgtattggt acgcttcagc ggcaagctgc
     9541 aaccgggcgt aaccctgcgc gatttggtga acgccatccc gctgtacgca atcaaacaag
     9601 gtttgctgac cgttgccaaa gccggtaaga aaaacatctt ctccggccgc atcctcgaaa
     9661 tcgaaggcct gcctgatttg aaagtggaac aagcctttga attgaccgac gcatccgccg
     9721 aacgctccgc cgccggctgt accgtgaagc tcaacaaaga gccgattatc gagtacatga
     9781 aatccaacgt cgtgttgatg aaaaacatga ttgccaacgg ctatcaagac ccgcgcactt
     9841 tggaacgccg catcaaagct atggaaaaat ggctggcaaa tcccgagttg ctcgaagcgg
     9901 ataaagatgc cgaatacgcc gccgtgattg aaatcaacat ggacgacatc aaagagccga
     9961 ttatcgcctg cccgaacgac ccggacgacg tgtgcttcat gtccgaacgc tccggcacca
    10021 aaatcgacga agtattcatc ggttcgtgta tgaccaacat cggccacttc cgcgccgcct
    10081 ccaaactttt ggaaggcaag gcagacaccc ccgtccgcct gtggattgcg ccgccgacca
    10141 aaatggacgc gaaacaattg tccgacgaag gacactacgg cgtactcgga cgtgccggcg
    10201 cgcgtatgga aatgccgggt tgctccttat gtatgggtaa tcaggcgcaa gtacgcgaag
    10261 gtgcgaccgt tatgtccacc tccacccgca acttcccgaa ccgtttgggt aaaaacacct
    10321 ttgtttacct cggttcggcg gaattggcag cgatttgctc caaactgggt aaaatcccga
    10381 ccgttgaaga atatcaagcc aatatcggca tcatcaacga acagggcgat aaaatctacc
    10441 gctatatgaa cttcaacgaa atcgacagct acaacgaagt agccgagacc gtgaacgttt
    10501 aatccccgtc atccgtatga agtaagggat tgaccgcaat gccgtctgaa caaccttcag
    10561 acggcattgc aacattccgc taacccttct ttccgcaaac gctgcaaata cggcgttcac
    10621 gcccccacat aaaggaaacg acagtgaacc tgaaaaaccg ccattttctg aaacttttag
    10681 acttcacgcc ggaagaaatc accgcctacc tcgaccttgc cgccgaattg aaagccgcca
    10741 aaaaagcagg gcgcgagatt cagcggatga aagggaaaaa catcgccctg atttttgaaa
    10801 aaacctctac tcggacgcgc tgcgcgtttg aagtcgccgc gcgcgatcaa ggcgcgggag
    10861 tgacttattt agagccgtcc gccagccaaa tcgggcataa ggaaagcatc aaagacaccg
    10921 cccgcgtgtt gggcaggatg tacgatgcca tcgaatatcg cggtttcggt caggaaactg
    10981 tcgaagaatt ggcgaaatac gcgggcgtac ccgtgttcaa cgggctgacc aacgagttcc
    11041 atcccacaca aatgcttgcc gacgcactga ctatgcgcga acacagcggc aaacctttga
    11101 accaaaccgc gtttgcctac gtcggcgacg cgcgttacaa catgggcaat tccctgctga
    11161 ttttaggggc aaaattgggg atggacgtgc gtatcggcgc accgcaaagc ctgtggccgt
    11221 ctgaaggcat tattgccgcc gcacacgccg ccgccaaaga aaccggcgca aaaattaccc
    11281 tgaccgaaaa cgcgcatgaa gccgtgaaga atgttgattt tattcatacc gatgtgtggg
    11341 tcagcatggg cgagccgaaa gaagtctggc aggaacgcat cgatttgctg aaagattacc
    11401 gcgttacgcc cgaactgatg gcggcatcgg gcaatccgca agtcaaattc atgcactgcc
    11461 tgcccgcctt ccacaaccgc gaaaccaaag tcggcgaatg gatttacgaa accttcgggc
    11521 tgaacggtgt ggaagttaca gaagaagtat tcgaaagccc cgccagcatc gtgttcgatc
    11581 aggcggaaaa ccgtatgcac acgattaaag cggtaatggt cgcggctctg ggcgactgac
    11641 agaactgtgc ctgtttaaat tcatccgcaa cacagatacc gtctgaacac gatgttcaga
    11701 cggtatccat atatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
    11761 ctatttgtac tgtctgcagc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
    11821 aaactgccta cacgatgtgt aggcagtccc gtttgaaaac aatcagtttt tgtcttggtc
    11881 aaccaatttg ttggcagtaa tccaaggcat catggcacgc agttgtgcgc cgactttttc
    11941 aacttggtgg tcggcattca aacggcggcg ggcagtcata gacgcgtagt tgacgttacc
    12001 ctcttggata aacatttttg cgtattcgcc ggtttggatg cgtttcagag cattgcgcat
    12061 ggcttctttg ctggaagcat tgaccacttc agggccggta acgtattcgc cgtactccgc
    12121 attgttggaa atggagtagt tcatattggc aataccgcct tcgaaaatca ggtcaacaat
    12181 cagtttcatt tcgtgcagac attcgaagta agccatttca ggcgcgtaac cggcttcggt
    12241 cagggtttca aaacctgctt tgatcaactc gaccacgcca ccgcacaata cagcttgttc
    12301 gccgaacaga tcggtttcgg tttcttcgcg gaaagtggtt tcaatcacac cgcctttggt
    12361 gccgccgttg gcagccgcat aagacagggc gatgtctttg gccttgccgg aattgtcttg
    12421 gtaaacggca atcagagaag gcacgccgcc gccgcgtttg tattcactgc gtacggtatg
    12481 acccgggcct ttgggggcaa ccataatcac gtccaagtcg gcacgcggaa cgatttggtt
    12541 gtagtgaacg ttgaagccgt gtgcaaatgc cagcgttgcg ccttctttca gattggctgc
    12601 aacttcggcg tgatagacgg caggcatggt ttcgtcaggc agcagcagca taacgacatc
    12661 ggcttctttg gtcgcttcag caacagtttt gacgacatgg ccggctgctt cggctttttt
    12721 ccaagaagaa ccttggcgca gaccaatcac cacgtttaca cccgaatctt tcaggttggc
    12781 agcatgggca tggccttgcg aaccgtaacc gatgatggca acggttttgc ctttgatcag
    12841 ggacagatcg gcatctttat cgtaatagac ttgcatttga tttcctttaa ggtaaatggt
    12901 tgtcgaagcc ttaaaatgtt gagcggcttc ggacgggtta aacagagtgt gccgcttaag
    12961 cggcaacttc attcatcaat acgatttcca acgcttcggt tttgccgtcg acggactgga
    13021 cgaaggcttg gaaatgcgcg ctggcattgt gttcgtcaat agctgcttgg gatttccaat
    13081 tttccacgaa aacaaaacgg ttcggtttgc cgatttcctg atggagatca tagctgatgt
    13141 tgccctcttc cgcacggctg gctttgacca gttctttaaa ctgtgctgcc agtgtttctg
    13201 tgtattccgg tttaacggta accagtgcga caattttaat gttcgacata aatctctcct
    13261 gccgttcgtt tttcagacgg cattcaaata ccgtgccgtc tgaaaggtta cggcgttaaa
    13321 ttttcaaaat acgctcaccg cgaccgatgc cggccgcgcc tgtacgtacg gtttccaaaa
    13381 tttgggcgcg tccgaccgtt tccaaaaagg aatccagctt gtctgtcgag ccggtaattt
    13441 caatcgtata gctgcggtcg gttacgtcga tgatgctgcc ccggtagatt tcggtcaagc
    13501 gtaaaaattc gtcgcggtct ttgccggcgg cacggacttt taccaacatc agttcgcgtt
    13561 cgacaaaacg gctttcgttc aaatcgacca ctttaatcac ttcaatcaat ttattgagtt
    13621 gcttggtgat ttgttcgata acctgctcgt cgccgtgtgt aacgatggtc atacgggaca
    13681 gggttttgtc ttcggtcggc gcaaccgcca aagaatcgat attgtaatcg cgtgcagaga
    13741 acaaaccgac cacgcggctc atcgcacctg attcgttttc gatcagaaca gataagatat
    13801 gtcgcatttg cctctcctta cgcctttccg tccgcacgca tatgcggcgg aagtaccatt
    13861 tcgtccaaac ctttgccgtt gccgaccatg ggcatcacat tctgtttctg gtcggtcagg
    13921 aagtcgataa acaccagcct gtctttttgg ttcaatgctt ccaacaacgc accttccaca
    13981 tcagacttct tgtccacgcg gataccgata tggccgtatg cctcggcaag tttgacgaaa
    14041 tcgggcaaag aatcgaaata ggtttccgac tctcgtccgc cgtaatatat ttcctgccac
    14101 tggcgtacca taccgagata accgttgttc agcgtaatga cgttaaccgg aatccgatat
    14161 tggaaacagg tggacagctc ttggatgttc atctggatcg agccgtcgcc ggtaatacag
    14221 aatacgtctt gatccggggc ggcaagtttt gcaccaatcg cataaggcag accaacgccc
    14281 atcgtaccca aaccgccgga gttgagccat tggcgcggac gttcgaaggg ataatattga
    14341 gccgcaaaca tttgatgctg ccctacatcc gatgtgatga ttgccgaatt gccggtaatc
    14401 tcggcaagct tctgaatcac atattgtggc ttgataattt cgctgccgtt gtcaaaccac
    14461 aagcaatcgc gggaacgcca ttcctctatg gttttccacc atttgcccaa agcatcttca
    14521 gacggcacgg actcttgttt ttgccacagc gcaaccatct cggacaaaac gtttttcacg
    14581 tcgccgacaa tcggaatgtc cgccttcacg cgtttggcga tgctggaagg atcgacatcg
    14641 atatggataa ccttcttcgc cttctcgaaa aatttggacg gtacggaaac cacacggtcg
    14701 tcaaaacgcg cacctacggc aagaacgaca tccgcattct gcatggcaag gtttgcctcg
    14761 taagtaccgt gcataccgag cataccgagg aattggcggt cgccggaagg ataagcgccc
    14821 aagcccatca gcgtacccgt gcacggagca cccgtcattc ggacaaatcg ggtcagctct
    14881 tcagaagcat tacccaacac cacgccgccg ccaaaataga cgaccggacg tttggcagat
    14941 gccaacatct gcacggcctt tttaatctga ccgatatgcc cttgaacaac cggttgatac
    15001 gaacggataa aaatgtcttc ctgaggatag ctgaatttcg ccatcgcctg cgtaacatct
    15061 ttcgggacat caaccaccac gggccccggt cggccgcttg cggcaatttg gaacgccttt
    15121 ttaatggttt ccgccaactc attgatgtcc gtaaccagga aattgtgttt gacgcacgga
    15181 cgggtaatac ccaccgtatc aacttcttgg aacgcatccg taccaatcag ggaattgcct
    15241 acctgcccgc tgatgaccac catcggaatc gaatccgtat aggcagtagc aataccggtc
    15301 agtgcattgg taacgcccgg gccggatgta accaatgcca cgcccacctt accgctgacg
    15361 cgcgcatacg catctgccgc gtgtactgcc gcctgctcgt gacgggtcag aatgtgcttg
    15421 aatttattga gttggaaaag ggcatcgtag atttcgataa ccgcaccgcc gggataaccg
    15481 aaaacgtact cgacaccttc ggctttgaga ctctgcacta tgatttgcgc gcctgatagc
    15541 tgcataacga cctcttttat acggtttcaa accaataggg acaaaccgct ttgccacagc
    15601 acctgtaatg caattccacc aagcagcgat ttagggtacg cgcattgggg gaacacggca
    15661 acagacggat tatccaatca attggaaagg aacacagagt ttgtgaaaaa gagtagaaac
    15721 gataacgcaa accgacagtt caatcaagaa aaatctttca tcttttaatt attttttgaa
    15781 agcagagaaa ttattgattg attttaaaag aataaaatca ggagtacctt ttttgaaaga
    15841 tggaaattgt tgacagtttg tgtaggaggg gcagatgtga aaaacccttc ttcgatatca
    15901 agaattgtaa aatttacagg gtttcatccc aataaagact cgggatattg attgaacttg
    15961 attttatttt tgatatatca aaaatattcc caaccatact tcctgaaaat ggctcattgc
    16021 accggactgt attggacggc attaacagaa ccaagagggc taacaacgac ttaatatatt
    16081 ga
//
LOCUS       DAWFKN010000041        16031 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFKN010000041 DAWFKN010000000
VERSION     DAWFKN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.041"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(392..1147)
                     /locus_tag="WGR46_001260"
     CDS             complement(392..1147)
                     /locus_tag="WGR46_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0856203.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(1327..2487)
                     /gene="nirK"
                     /locus_tag="WGR46_001261"
     CDS             complement(1327..2487)
                     /gene="nirK"
                     /locus_tag="WGR46_001261"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0856204.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2857..5112
                     /locus_tag="WGR46_001262"
     CDS             2857..5112
                     /locus_tag="WGR46_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0856205.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5376..6080)
                     /locus_tag="WGR46_001263"
     CDS             complement(5376..6080)
                     /locus_tag="WGR46_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856206.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            6297..6560
                     /locus_tag="WGR46_001264"
     CDS             6297..6560
                     /locus_tag="WGR46_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0856207.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6638..8683
                     /locus_tag="WGR46_001265"
     CDS             6638..8683
                     /locus_tag="WGR46_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856208.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDVWVGDGHSFKAKIRHPMGYLFTPEVTMIVDGCSGAVVGW
                     SVALSETAVAVADALRHGMTHFPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFT
                     GIPGNPQGRGKIERLWQTITIPLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGK
                     ELSKAQEAALSAAPTWAQFIADLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYR
                     METDGMRVKTDVLSPLELDFMYRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVA
                     YDIHDADHVIVKDMQGKVICKAVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDL
                     IEAQRQSAAPIIEQQPDFGEFLKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8725..9639
                     /locus_tag="WGR46_001266"
     CDS             8725..9639
                     /locus_tag="WGR46_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0856209.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9715..9957
                     /locus_tag="WGR46_001267"
     CDS             9715..9957
                     /locus_tag="WGR46_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856210.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9947..10246
                     /locus_tag="WGR46_001268"
     CDS             9947..10246
                     /locus_tag="WGR46_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856211.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            10249..10401
                     /locus_tag="WGR46_001269"
     CDS             10249..10401
                     /locus_tag="WGR46_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856212.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10417..10581
                     /locus_tag="WGR46_001270"
     CDS             10417..10581
                     /locus_tag="WGR46_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856213.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10562..10777
                     /locus_tag="WGR46_001271"
     CDS             10562..10777
                     /locus_tag="WGR46_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856214.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10774..11088
                     /locus_tag="WGR46_001272"
     CDS             10774..11088
                     /locus_tag="WGR46_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856215.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            11207..11584
                     /locus_tag="WGR46_001273"
     CDS             11207..11584
                     /locus_tag="WGR46_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856216.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11568..12023
                     /locus_tag="WGR46_001274"
     CDS             11568..12023
                     /locus_tag="WGR46_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856217.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11995..12189
                     /locus_tag="WGR46_001275"
     CDS             11995..12189
                     /locus_tag="WGR46_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856218.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            12182..12793
                     /locus_tag="WGR46_001276"
     CDS             12182..12793
                     /locus_tag="WGR46_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ0856219.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12799..13449
                     /locus_tag="WGR46_001277"
     CDS             12799..13449
                     /locus_tag="WGR46_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856220.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13450..13650
                     /locus_tag="WGR46_001278"
     CDS             13450..13650
                     /locus_tag="WGR46_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856221.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13666..13974
                     /locus_tag="WGR46_001279"
     CDS             13666..13974
                     /locus_tag="WGR46_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0856222.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNG
                     "
     gene            14050..14484
                     /locus_tag="WGR46_001280"
     CDS             14050..14484
                     /locus_tag="WGR46_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ0856223.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14481..14927
                     /locus_tag="WGR46_001281"
     CDS             14481..14927
                     /locus_tag="WGR46_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856224.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14975..15616
                     /locus_tag="WGR46_001282"
     CDS             14975..15616
                     /locus_tag="WGR46_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856225.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15748..>16031
                     /locus_tag="WGR46_001283"
     CDS             15748..>16031
                     /locus_tag="WGR46_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0856226.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTV"
BASE COUNT         4151 a         3522 c         4606 g         3752 t
ORIGIN      
        1 ggaatgacgt ggtgcaggtt tccgtgcgga tggattcgtc attcccgcgc aggcgggaat
       61 ctagacttta gaacaacagc aatattcaaa gattatctga aagtttgaga ttctagattc
      121 ccactttcgt gggaatgacg aaaagttgcg ggaatgacga aaagttgcgg gaatgacgaa
      181 aagttgcggg aatgacgaaa agttgcggga atgacggaaa gtggcgggaa tgacggaaag
      241 tggcgggaat gacggttcgg gcattcctta aatcacccgt gtatcgctgt aaatcttaga
      301 gatggcggaa tatagcggat taacaaaaac cagtacggca aggcgaggca acgccgtact
      361 ggtttttgtt aatccactat aattgaaggg gttatcggct tgcgcaacgg aagcccaagt
      421 tgtgcaagac atatttggat tgcaggctgg tgcggatgcc gtagcggagg aaggcggcat
      481 agttggacga gtcgctcgac ccaatagacg cgccgctgca aaacatttgc gcattggcat
      541 ttccggagga aagcaggctg ctgttgaaat cttccgtcca ttcccaaata agtccgtgca
      601 tatcataaac gccccagtag ttgggacggc ccttgccgac gtcatgcaag ccttttcgtc
      661 cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc gggttcgttt gagccgtttt
      721 tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc aatggtcggc aggcgtttgc
      781 cttgtgcggc gcaataggcg ttggcggcaa accaggaaac attggttacc ggttgtttta
      841 attcgcccgc cttcggcgca tagctgcggc tgccgttttt catccaatgc ttcaggtaag
      901 cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca ttgggggtgg ctgttgacaa
      961 attcggcaaa ctcggcattg gtaacgggat atttatccag tttgaacggt ttgactttaa
     1021 tcaggccggt atcttttttc agataaagcg ggcggtagct gccgccttcg atttgaacca
     1081 tttcggcagc tgccgcttga gtgccggcga gtgccgcaca gagaaagagt aaccgggtaa
     1141 acttcatatc atgcctcctg acaggcagtt aaaattgatt tttcagacgg cattttaaac
     1201 aaaaagccgc gaatgccgtc tgaagaatca atcttccgaa aggaaagatt ggttgttaaa
     1261 aaaccactgc cgtgcgtaat taagtacggc ggcagtggtt cgtttgccta aaaatgggtg
     1321 ttcaatttag taatcgcttt ttgcaggagc agaggctgca ggagcagaag ctgcgggagc
     1381 ggaagcagca ggagctgcac cgttaccggc gtaagcggta tcactcaatt tttgagtcat
     1441 gatttcaggg ttttctgcac cctctacttt caattgaccc agtgcgcctt tgttgaatgc
     1501 gcggaagata gagtggtcaa ccaaagtgta gctgcccggg atgtcgactt tgaattcgac
     1561 gatggcagag ccgccggcag gaacgatggt gctttgtacg ttttcgttaa tcagtttgcc
     1621 gccttcaaca taaactttgt cgaagatttc accgattacg tggaaggaag acaccaagtt
     1681 aggaccaccg ttaccaacgt acatacgtac agtttcgcct gctttggctt tcagggcgtt
     1741 gtcgccggcg atagcaccta cgtgaccgtt aaatacaacg tattcaggtt gttcggcgat
     1801 ggctttgtcc atatcgaacg gttgcaggcc ttgcgcgcct tttttgcctt tggtgtagaa
     1861 gtcgccttgg acgatgtaga actctttatc cactttcggc agaccttctt taggctcgac
     1921 caaaatcaga ccgtacatac cgttggcgat gtgcataccg accggcgcga cggcgcagtg
     1981 gtagatgtac agacccggtt gcagggcttt gaagctgaat gtggaagtac ggcccggagc
     2041 ggtaaaggtt gcggccgcgc cgccaccttg gccggtagct gcatggaagt caacgttatg
     2101 gggaacggta gaagaaggat tgttagagaa ttctacttct acggtatcac cttcgcgtac
     2161 gcgaatcata cggcccggaa cgtcgccgtc aaatgtccag tagcggtatt ccaccccgtc
     2221 gtccattttc atggtttttt cgacggtttc catttttacg cgtactttgg caggatagtc
     2281 gcggtcgatt gcgggaggta cttcaggagc gtgggtggta actgcatcga tgacgggcag
     2341 ttcgcctgca ggcgtttcgg cggcggtttg tgcggcggag cttgcggctt cggcggaagc
     2401 ggcaggggtt tgcgcggcag gttcgccacc gcaggcggct aaagcaaaca tagaagcgat
     2461 aattgcggct aaggcttggc gtttcataat gttttccttt tgtaagaaaa gtagggagat
     2521 tggttaagaa ctggtgtgat tatgcgtttg ccccatcacc gacattttga tttaagtcaa
     2581 ataattcata aaatatgaac ttaaaatgat acttctaata ctttatagct actaaaaatt
     2641 acaataaata ggctgatttg cttaagggct tcggcaaatc aggcaaaggc agggtagttg
     2701 ccgactaaag aataatgata gttattatca tatatgtatt tgttttatat gtgagtttct
     2761 ttgaacaaat gtttatttgc gcggtaaacc gtgctacaat ctttaacatt catattttgt
     2821 gaattttaat ccactatatt aaaaggagct ctcaaaatgg gacagtacaa gaagctgtgg
     2881 tacttgctgt ttgccgttct ggcggtatgc tttaccattc ttggctatat gggcagcgag
     2941 gtttataaga aagccccgcc ttaccccgaa caggtcgttt ccgcatcagg caaagtgctg
     3001 atgacgaaag acgatatttt ggcaggtcag tctgcgtggc agagtaccgg cggtatggaa
     3061 gtcggttcga ttctgggtca cggcgcatat caggctccgg actggacggc ggactggctg
     3121 catcgtgagt tgtccgcttg gttggatttg accgcgcaac agacttacgg caaaaaattc
     3181 gatgaagttt cccctgaaga acaagccgtt ctgaaaaccc gactggctga cgaataccgc
     3241 aaccaaagcc gtattaaaga agacggcagc gtcgtcatca gcgatacgcg cgcgaaagcc
     3301 atcgaaagca tcctgcctta ttaccacggt gtttacggcg acgatcccgc gctgcaaacc
     3361 acccgcgaac actttgcaat gaaaaacaac acattgccaa gtcaggaagc gcgtgaaaaa
     3421 ctgttcgact tcttcttctg gacttcttgg tctgcttcga ccaaccgtcc cggcgaggtc
     3481 tttacctaca ccaacaactg gccgcacgag cctttgatta acaatgtacc gactactgaa
     3541 aactacatgt ggtcgtttac cagtgttgta ctgctcttga tgggcatcgg tttgttgatg
     3601 tggggttatt ccttcctgac caaacacgag gaagtggaag tgccgtctga agacccgatt
     3661 tccaaaatcc aactgacccc ttcgcaaaaa gcattgggca aatacgtctt cctgacggtt
     3721 gccctgtttg tggtacaagt attgctgggc ggtctgactg cgcactacac cgttgaaggt
     3781 cagggtttct acggcattga cgaggcgttg ggctttgaaa tgtccgactg gttcccctac
     3841 gccctgaccc gtacttggca catccaatcc gccatcttct ggattgcaac cggctttttg
     3901 acggcgggtt tgttcctcgc gccgattgtc aacggcggca aagatcccaa gttccaacgt
     3961 gcaggcgtaa acttcctcta tatcgccctc ttcatcgtgg tcggcggttc ttacgcgggc
     4021 aacttcttcg cgttgacgca catccttccg ccggaattta acttctggtt cggacaccaa
     4081 ggttacgaat acctcgattt gggacgtttc tggcaactcc tgctgatggt cggcctgctg
     4141 ttgtggctgt tcctgatgtt gcgctgcacg gtttccgcct tcaaagaaaa aggcgtggac
     4201 aaaaacctgc tggcaatctt tgtcgcttct atggtcggtg tgggcgtatt ctacgcacca
     4261 ggcctgttct atggcgaaaa atccccgatt gccgtaatgg aatactggcg ttggtgggtg
     4321 gttcacctgt gggtggaagg cttctttgaa gtatttgcca ctgccgcttt tgcctttgtc
     4381 ttctacaata tgggctttgt ccgccgcagt accgccacgg cctctactct ggccgctgcc
     4441 gccatcttta tgttgggcgg cgttccgggt acgctgcacc acctgtactt ctccggctct
     4501 acctccgcct ctatggcaat cggtgcctgc ttctccgctt tggaagtcgt gccgctggtg
     4561 ttgctgggcc gtgaagcata cgagcattgg tcttaccaac acctgtccga ctgggcgaaa
     4621 cgcctgcgtt ggccgctgat gtgcttcgtg gcagtcgcct tttggaatat gatcggtgcc
     4681 ggtgtattcg gtttcctgat taacccgccg atttccctgt tctatatcca aggtttgaat
     4741 acttctgctg ttcatgcgca cgccgccttg ttcggtgtgt acggtttctt ggcattgggc
     4801 ttcgtattgc tggttgcccg ttatttgaaa ccgaacgcgc agtttgacga caaactgatg
     4861 acttggggct tctggctgct caacggcggc ttggtcggta tgattgccat cagcctgttg
     4921 ccggttggcg tgattcaggc atacgcctcc atcacgcacg gtctgtggta tgcccgcagc
     4981 gaagagttcc tgcaaatgga aatcctcgat accttgcgtt gggtacgtac ggcagctgac
     5041 ttggtcttta tcggcggtgc aatctgcgtt gccatccaag ctactaaaat cgtattcggc
     5101 cgcgacaaat aagttttgcg gtatgaaatg agcaacttat ggggtaggta ttattttgac
     5161 acctttagaa agaaagcaaa agaaggcgaa aattttaaag gaaacaaaaa tttgaacgga
     5221 aatttaggaa aaagcggctt tgacaccttt aggtgcaaag taggtagaaa gtgatataac
     5281 tttgatacct tttttgcacg gttttgatga ggttttgggt tgattttggt tttatttgaa
     5341 ttgaacggcg tttggaatat ccaaacgccg tttttttagt tgacggtacg gccataccat
     5401 tctacgcgtc cgatgatggc tacattttgg ttgtcgtcgg ttaaatctat ttcgaaaggc
     5461 tcataaaccg gattggctga tttgacgagc agcttgccgg gtagtttttg gatttgtttg
     5521 acaaacaggt gattatcgat gcggataacg tacaggccgt cgcgcggggt ggtttcggcg
     5581 tgattcacta ggatgttgtc gccgtggttg agtacgccct ccatcgaatc gccctttacg
     5641 gcgatgacgg acagtttgtc aagctggcgg gtaacgtagt tttctatcca ataacgcctg
     5701 aatgccatgc agaataaggg cttgtcgtct tctgcgggat agccgtgccc tgcggcggcg
     5761 tagacgtcgt aacgcgggat aaagacgaac tcgtctaagt tgatggggtt gccgagggta
     5821 tcggtagccg taccgtcggt atgggtacgg acttcgaccg cttggccttt attcatgtat
     5881 ggcgagcctt ggccggtaag aagccagttt aaatcgcaac cggttacttc ttgaatttta
     5941 atcagatagt ctgctgttgg aatggcaccg tctttccaga cacggctaaa accggaggca
     6001 gacatatcaa tcttattgta gaagtcggcg ggtttggcat tgtccggcca caaagatttc
     6061 aaacgctcta aaaagtccat gattgtcctt ttgcttagaa tttcgcattt aagcaaaaaa
     6121 tacacttctt ttgcttaaaa ttaattcgtc aaataatcaa atagatggct aatattttgc
     6181 ttttttgcgt tatatttcta agcaaaagag ttgcaataac cttttttgct tagtattatt
     6241 cactcactta atcaattaac gaagtaaatc acattactga atggaataag tcaattatgc
     6301 aaaaaaacgc aacgccgaaa aactggcacc gcgcggatat tgttgctgct ttgaaaaaga
     6361 aaggctggtc gcttcgagca ctttcaatag aagcggggtt gtcgccgaat acgcttagaa
     6421 gcgcactggc cgccccttat cttaagggag aaaggattat tgccgctgca atcggagtgg
     6481 aaccggaaga gatttggccc gaacggtatg cagatcggaa tttaaaacct gttttcccca
     6541 aaagagtagt taatggataa gtggttttcg atttagttcc ttaaatgcta aaggttatac
     6601 gcatttaagt aaaacacaaa aagaaagaaa tagcaaaatg ttgatttcag cggttgagtt
     6661 ggcgggcttg caacttccaa agctgccgaa cagtagacaa ggcatcgagt accacgccaa
     6721 aaagaataat tggccgtttg aagaggttgt aggacaggct cgaggcggca agcttaagaa
     6781 atatttggtt tccgccctcc cttcggagat tcaggcggcc atacaggaaa aacaggcggc
     6841 aacattgttg gccaaggtgc cgatgttgcc ggcagaagta aaaaaaccgc ttcggcaaaa
     6901 taaaaagatg cggcagttgg gtttgatacc gtgcgaggaa ggtttggcgc ggttggacga
     6961 caggcagacg gaaacggccc acgcgcgttg tgcgattgtc gcctatgtat tgccgctgca
     7021 tgaattggcg ggtatgccga ttaagaaggc ggtggcgttt gtggcagctg aagcggcagc
     7081 aggcaggttg ccggaggatg tggccaagct gattccgttg gcgaatgcgc ggaacaacgg
     7141 cgaacgcagt ttaagcgaac cgactttata ccgatgggtg cgggcttacc gtgcggcatc
     7201 ggacagtatg agccgcctct tggcacttgc gccggtgaaa acacgggaaa agacgccgct
     7261 gctggcgatt gactggctgc cttacttctt gatgttttat cagcggccga acaagccgac
     7321 gatgatggcg gcggcgaaga aactggcgca atggtatctg gaacagggaa aaatcgagca
     7381 gatgccgagc tatgaccaga ttcaaacagt gatgaaacgg ttgccggaac atatgaagga
     7441 gcgcggcagg cgcacggggt cggcttataa ggctttgctg ccttacattg atagagattg
     7501 gacggcgttg aaaccgaacg atgtctgggt gggcgacggc cacagcttta aagcaaaaat
     7561 ccggcatccg atgggctatc tgtttacgcc ggaagtgaca atgattgtgg acggttgcag
     7621 cggtgcggtc gtgggttgga gtgtggcgct gtcggaaacg gcggtagcgg tggcggatgc
     7681 tttgcgccac gggatgacgc atttcccgcc acctttgggt tattactcgg ataacggctc
     7741 gggtgaaacg ggcgatatgc tggataagga aacgacgggt attttgccgc gtatcggtat
     7801 cgagcatttt acgggcattc cgggcaatcc gcaggggcgc ggcaagattg aacggctgtg
     7861 gcagaccatt acgattcctt tggccaagca gtatgcgact tatcagggca aggatgcgga
     7921 tgccgaaaca ttgagaaagg tatcgaatgc gctggcgagt gcgcagaagg tgcagaaaaa
     7981 gggcaaggaa ctgagcaagg cgcaggaagc ggcgctgtcg gcggcaccga cttgggcgca
     8041 gtttatagcg gatttggaag aagcggtacg ccgatacaac tttgaacacg agcaccgcag
     8101 cctgccgaaa aatccggaag cgggacggca ttttacgccg atgaaatatt acgaataccg
     8161 gatggaaacg gacggcatga gggtgaaaac ggatgtatta agcccgctgg agttggattt
     8221 tatgtaccgt ccggaggaag aacgcatccc cgaccgcgga gcggtgtctc tgcacaataa
     8281 tacttacttc caccaagaat tgctggatta cagcggtcag aaggtacggg tggcttacga
     8341 catccacgat gccgaccatg tgattgtgaa ggatatgcag ggcaaggtga tttgcaaagc
     8401 ggtattcaac ggcaacaaac gggcggcatt tgcggaaacg cggatggaac aactggccga
     8461 acgccgccgc aaaggccagg ccaaacgtct gcaagacaag atggatttaa tcgaggcaca
     8521 acgccaatcg gccgcgccga ttatcgagca gcagcctgat ttcggcgaat ttttgaagct
     8581 ggaaacggaa agcggcgggt tggtagaggt ggaaaacagg ccgtctgaaa gcggcagggg
     8641 caagaagaag ttacgcgatt ttttatggga agacgccggt tagtaaaacg gcacatcttt
     8701 gacacttttt aaggaaaacg aaaaatgatt gagaaattaa gacaatttct ggaaaccagc
     8761 ggcatgagcc aaaacaaggc ggccgaccgc atgggcgtat cgagaagtgc attgtcgggc
     8821 tatttgaacg gcaaatacga cggcgatatt gcggggatgg ataagaaagc cgcgctgttt
     8881 cttgaacagg aaggcgaccg ggcggaattg aaaaagctgg atattcccta tgttgaaacg
     8941 ggcacggcca agaagatgaa aggttggctg ggtttggcgg catggctggg gcagctcggt
     9001 attgtgtatg gcggtgcggg cttgggtaaa accacggtat tgaaacaata tgcggccact
     9061 aacccgctgg ctttgttgat tgagccggat acaggctata cggcaaaggt gttgttgcag
     9121 gaaatctgcc atgcactgga tttaagcgag agaggcaata tccatgagct gacggagcgg
     9181 attatcaact gcctgaagcg tgataagaag agccgcagcc cgcgtgatgc acaccgtatt
     9241 ttgctgattg acgaggcgga acagttaccg acacgcgcac tggagagcct gcggcggata
     9301 catgataaat cgggcgtggc ggtggcgttg gtgggtatgc caaagctgct gctgaatctg
     9361 aaggggccga acagtgaatt caagcagttg ttttcaaggg tatcggtcaa gatggaattg
     9421 ggcgagatgc tgccggaaac ggatttaaaa cagattgcgg cggcagtgtt gaagacgaac
     9481 gatgaggcgg tattgcagaa ggtggtgaaa acggccaaag gcaatgcgcg gaaactgtcg
     9541 aagctgctgc tgattgtgga ctatttgttg caggttaacc ctgatgttga tttggatgat
     9601 gatgtaatcg aacacgcgga aacctattta atccactaaa cctttgacag ataaggcaat
     9661 aattttttta ccttgttatt ttgttaagtt attgaaatta aaggaaaact aaaaatggaa
     9721 aatttgaaaa tgcggcatct gctcgccata ccggcgattg tgattgcggt aacggcgtgt
     9781 gcgaaccgct gcaccgagcc ggtgccggcg tggagcctgt atcagaacga gaccagtcag
     9841 cagcggctga tgcgggaaaa acagaatgcg gctgactttg cggtattgca ggcagaaaaa
     9901 gcctatgaac ggatgagcga taaagagcgg atgaaagggg tggtttatga aaactgagac
     9961 gaaaatctgc aaaaaatgcg gagaagagag gccgttgttc cgtttcaaaa aagacgatgc
    10021 gtatgcgggc gggtataggc ctgtatgtta cgagtgttta aacgccagag caaaggaact
    10081 gaaggcagtc cgagacagta tggcggaaag aaagattatc tctaaaggca tcagcataaa
    10141 tggagggaaa acgcatatgg aatttcctga agaacctgaa tttatgaaaa acaaatactg
    10201 gaccgttaca aaaacggatt atagaagacc attaaaagga ctttaaccat gagtaaacgg
    10261 ttggcacgac ggaagatgcc gaaacacgaa ttgaaacggg ttaaagcgtt cgccctgaaa
    10321 caggcggtcg agaagattcg ggcacggtgg ggcgagaaag cgatacggct tgcctcaaca
    10381 gacaacaaac agactgatta aacaaggaat aagaaaatga tgaatgaaat gaatgcggta
    10441 gcggctttgt tggttattgc catattgctg gcggcggtaa tgactgtgtt ggtcgagata
    10501 ttggtcgagc aacgggttaa ggaacggctg gggaaactgc tggataaggc gtgccgaccg
    10561 gatgacgggt ggcaggaatg ataaggaagg aaatgtgatg aatatggtac tgacaattta
    10621 tttttggttg gtggggctgg tctgttttgc ccgttccgcc aaagagactt attacgcgga
    10681 atctttatac ggtctgctga ctgggctgtt gggggtgatg tgttcggctt ttgtggtgtc
    10741 agtgtcggtg gcaagggtga tggggtggtt gaaatgaatc aattgaaaat gacgatcgcc
    10801 aaacctgaga cggaagattt tgaagatgcc tgggcattta tcagaatgct taatttggtg
    10861 acttatgatt tgaaccctct gaaaactgat accgacggcg aatacgaata tttggcggat
    10921 gaagacaaat ctgatgtatt agatgccgtt gtcgaaaaat ttaacgaatg tagtcttgag
    10981 tggatgttgt cggcgctgca agcactgatg tcgccggaga tggggattat caaccaagat
    11041 tcggatacgt tggagttgca tcccaaactg aaaggcggta cggaatgaaa gtgcgctgcc
    11101 ctacctgcgg tgcggtgatg agcttagatg tattaatcgc ccatgatgat gcagcgaagc
    11161 tctgattgcc ctgacaggca tttcagacga cctttttaag gcggtattgc ggtatctgac
    11221 gctgtttcgc cccgccgaaa aggatttaag ttttaaccga gtttcaaagc tcgtcggcga
    11281 gattgcgccg atgatacggg acggaaagat tgtccgcaac cgcaaaactt atccggcccc
    11341 gcgcgaggct tggatttggg cggcaacgcg atgccttgag gcacgggacg cgggaaaact
    11401 gacaccgcca ctgaccagcc acggttattt gttggaaaac attacgtttt ggtcgcctga
    11461 aaagacggcg ggaacggcgg ttttgccctc tccccaacca tctcacacga gagaggtggc
    11521 aagcaccaaa ttgaggagcg ggttgggcgg cttgatggag tggtcaaatg gaggaaaaca
    11581 atagctggct gaaaaaagca atcgcgcagg gtttcatgat gctcgccgcc ctaaacctca
    11641 aaggccgccc tgcctcggcg gatttgacgg cagtcgccga actttggttg gtcatactaa
    11701 gcggccggtc gtggcagccg gagcatgacg ggatcaggat acaggcagcc tttagggcta
    11761 tcgcggcgtc ctcgtcagag tggccaaacc ctgccgacct tatcaagcac ctgccaccgg
    11821 gcgaagtcag gatggtgccg aggctggaaa agaagcactg cccgacggaa tacggcaaag
    11881 agcaggccgc cgaactgaaa aagattgtcg gcagattgaa aaacgcaccc tgcatgaata
    11941 gagattggat acacgggcaa cgccaccggt cggtggacga atgtaaaagg atttatgccg
    12001 aaaggcagaa gggaaataaa tagccaaatg agtacgtttt ttgacaatca ggaatccgac
    12061 ttaataaacg aatttctgac ggaatactgg gaagagtttg aaaaattctc cgccaacaac
    12121 ggatttgatg acgatttatt gaagcaaata tcaatggaat tggaaggaca taggaaccat
    12181 tatgagtaac ttagacatga aacaatacaa acaggatgcc aaaggcaatc tcgtgccgct
    12241 ggccaatatt aaagaaatcg acctgctgcg cgacgagctg gtgcaggaaa ttgccgccaa
    12301 agcccgcgcg gtacaggata acttgatggc gttcaaacgc gaggcgatgg acgatatcgc
    12361 agcgtttgta cagttgagtg ccgaccgcta tgacgtatct gtcggcggta agaaaggcaa
    12421 tatcagcctg cacagctttg atggagcgta ccgcgtcaac cttgccatac aggacacgtt
    12481 ggtatttgac gaaggtttga ttgccgccaa agccctgatt gacgagtgta tcaacgaatg
    12541 gacggaaggc agtcgcacgg aattgaaaac actgattaac gcggccttcc aagtggacaa
    12601 agaaggcaat atcagcactg cccgcgtcct cggtctgcgc cgcctgcaaa tcacggatga
    12661 aaaatggcaa cgggcgatgg atgcgctctc cgacagtttg caggtgcata tcagcaaacc
    12721 gtttgtgcgg gtatatcagc gcggcgagga tggggagtat cagttaatga atttggatgt
    12781 ggcgaaggtg taggaaaaat gaacgcaaat ataatgattt atttgattga agttaatgat
    12841 gggccggttg caaaagcact gcgaagtttt cagcaggaca aggccgcaat gcggcaggcg
    12901 tggcttgatt gggcgcgaga acacttgccg tcagacgcgc taatgcggga atggagcgat
    12961 gggcaagtgg cagggtttgc gttcccttca ggcatacccg atggatggaa aaagcccaat
    13021 aagaacggaa tttgttggcc acggcaaaac aacccaatcc tcaaaaccat gccacttaac
    13081 aaaagattta aacgccccga agagtatttg gaagaagttg gcatcaccgc accaacaatg
    13141 atttttgaaa agaatagcga cggagagact tgggcatctt ggggtatcgg taatttcttt
    13201 aatcctgttc agttcgtttg ggcgggattg gaggaggacg cgccaaaggg cgtggtaact
    13261 cctgattatg cctacgagct tagagaaggg gctaagagaa tcagaaacgg ctgtacgatg
    13321 cagccgcccg aagattttga ttggcagaat ctgttaccgg gctgccgtgt cattcctcgg
    13381 tatgagtggg attacttggt tgggaaatgg caggaaacac ggaatgatgc cgaagaacag
    13441 gaggcttaaa tggcaaaaat cattattgaa atcgaagacc tgcccgaggg caccgacatt
    13501 aattttaagg gcgaccttcc ggcggcggat gcaaaagaca aaacaggtgc gcagcaaacg
    13561 gctgtgttaa tcagcaagat gattcaggcg gcgcagatga tggcatcccc atccgtaaac
    13621 attaggccgt ctgagactgc aaatccataa cccgcgcggc acggtctgcc gcatttaaat
    13681 ctaaatagga gtcaaaaagt gaataaatcc gaattaatcc aagccatcgc cgacgaggcg
    13741 gaattgagca aacgcgctgc ggcggagttt gtcaatgcgt ttgtcagcgt ggtaacgcaa
    13801 gaactgaaag acggaaacga cgttacgctg gtcggcttcg gcacgttcca cgccgcccaa
    13861 tccgccgagc gtcagggacg caatccgaaa acgggcgaac cgctgaccat cgcggcgcgc
    13921 aaaacaccca agttccgctc gggcaaggcg ttaaaagatg cggtaaacgg ttaaacgtct
    13981 gaaaccccaa atccaagcgg atttttgaaa agtccgcttc agtttgaagt tttagcgacg
    14041 aggaaaacca tgatgaacac aaacgaacag tacaaacgca aggggctgat tgcgaaaatc
    14101 aaaatcgcgc aaagccagct tgggatggaa gacgatgttt accgtgcgat gctggcgcgt
    14161 gtgtgcggca aaacatcctg cacgcagatg aatctagccg aattgcaggc ggtggcggcg
    14221 gaaatgaagc ggatgggctt taagcagacc gcaccgaagg gcaaaggtat ccgcccgcat
    14281 ttgacacagg accgtgccgc gctgttgaac aagctggaag cgctgttgac ggtgggcgat
    14341 aaaagctggc agtacgccga cgggatggcc aaaaggatgt tcggcaagga tttggtacga
    14401 tttttgacac ccgagcagct atacaaactg gtgcaggcgc tgcaaatcca tatcaacaaa
    14461 gcgaaaaagg cggcagagca atgatcaggg tggatgaaga agattttgag gcggtgcgcc
    14521 acctgctgcc cgaaagtgtg ctggcactga ttacgatcat cgggctgaca gaaacggtgg
    14581 agctggtgaa aaacttaggc ggcacgacct acccgttgcg gcagggacgc accaaaggca
    14641 gcgaatcgcg gctggcttat ttggaggaaa tcgtcggcgg tgcggcgatg gaaaaaatgg
    14701 tggaagcact ggccccctgc gatttgttta ttccgaaatg cgagcaggct ttgctggaat
    14761 tgcgtgaccg ccatatccgc cgccgcttcg atgcgcagac gggtaaaggc gtaccggctt
    14821 atgaggcggt gaacgatttg gctttggcgc acgcgctgac cgaccgccat atctggcgga
    14881 ttctgaagaa gccggataat gagtgtgagc aaggcgggtt gttttaaatt ataatgccgt
    14941 ggggataaca cggcattttt tatgggaaat caagatgaag acgttttatg ttctgatgtt
    15001 ggccgcaatg cttgcggctt gcggcggaca agaaaacaag cctgctgccg gcgaaagtca
    15061 aaccgataat cgggcgcagc aatatcagaa tcagcaaaaa cagattgccg cacgtttggc
    15121 tgatgaagaa tatttcatcg gtgaaaacaa tttggaacaa atccgtctgc acgccaattt
    15181 gaaagagcgc gctcaaaagc tgttgtcttt attggcgcag gccgacaagg aaagccgtgt
    15241 gtgggttttg cccggcgatg cagccaaaat caaagaattc aatgcggctt tcgcggcggt
    15301 ggcaaaatct gccgaggaat ctttcggcgg cccttttctt gcggataagg cggggcttta
    15361 tcaatgtacc gctgcggcca atgcggctta cgattatttt ttcgcccggc agagaaagga
    15421 tgcgctgacc gaaaattacc gtcaacaata cacggacaac atagctgcct gccaagggca
    15481 aatccgcacc ccgccagccg cccaagcgac agtgtatgcg cgtaaaggta tcaggctgcc
    15541 gttgaataat tgtttgccgg tattggccgg agatgaagaa tttgatacct acacctgccc
    15601 gatggaagta aaataggccg tctgaaaccc agcccgagag caaaagccct cgggcttttt
    15661 gctgcctgtc tgacatgggg caggcgcggt gccaaataag cggacaggat aatcaaggct
    15721 cgataacaga ctaagcagga cgaatctatg agcaagatta ttgtgctgac cgcaggccac
    15781 agcaacaccg acccgggtgc ggtcaacgga agcgaccgtg aggcggactt ggcgcaggat
    15841 atgcgcaata tcgtggctgc tattttgcgc gatgactacg gtttgactgt taaaaccgac
    15901 ggcacgggca aaggcaatat gccgctgcgc gaagcggtca agctgattcg cggctcggat
    15961 gtggcgattg agtttcacac caacgctgcc gtcagcaaag cggcgacagg catcgaagcc
    16021 ttgagtaccg t
//
LOCUS       DAWFKN010000042        15991 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFKN010000042 DAWFKN010000000
VERSION     DAWFKN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.042"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            275..1300
                     /locus_tag="WGR46_001284"
     CDS             275..1300
                     /locus_tag="WGR46_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0856227.1"
                     /translation="MKYKALSLLPLVAALAACAGGGVAEPHVPVSIPTATPLPKGEVT
                     LSSDNGKIENINTTSTGSTSVISIQERAVTKNYFGVESQEKSFIFKTPGGAQYTLSSY
                     ADPITVSYSSPDFKIPDHHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIG
                     PNGEIDLFAGGFPVGKTPPPAFSYGSSTPETKGTGKITYQVWGIRVRNGQFVTSSYTP
                     PKNSGYTFNPTNTPVLSFITANFNSNKLAGKIIGNSDYGPDVEIKEAQIDGLSFSGDA
                     TSGGKTGKLEGKFFGKFNSSYDSDTSIGGKITFDGDRSLDTVFGGVSYKKELESTTDR
                     ETTHLTK"
     gene            1331..3763
                     /gene="hupB"
                     /locus_tag="WGR46_001285"
     CDS             1331..3763
                     /gene="hupB"
                     /locus_tag="WGR46_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0856228.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGNVEIKNKGYVYDPNKPFNPSLGPSSYVATGVARSQPDPQEWVNKSTLFK
                     LGYNFNDRNRIGWIFEDSRTDRFTNELSNLWTSLTGDITGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKDYDTNGINGQVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGKGDNANSDYSYFAKLYDPKILTTNQGKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGRATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4370..6004)
                     /gene="groL"
                     /locus_tag="WGR46_001286"
     CDS             complement(4370..6004)
                     /gene="groL"
                     /locus_tag="WGR46_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0856229.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(6097..6387)
                     /locus_tag="WGR46_001287"
     CDS             complement(6097..6387)
                     /locus_tag="WGR46_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0856230.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            6616..7269
                     /locus_tag="WGR46_001288"
     CDS             6616..7269
                     /locus_tag="WGR46_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0856231.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            7482..9017
                     /locus_tag="WGR46_001289"
     CDS             7482..9017
                     /locus_tag="WGR46_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0856232.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            9014..9106
                     /locus_tag="WGR46_001290"
     CDS             9014..9106
                     /locus_tag="WGR46_001290"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0856233.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(9166..10386)
                     /gene="lysA"
                     /locus_tag="WGR46_001291"
     CDS             complement(9166..10386)
                     /gene="lysA"
                     /locus_tag="WGR46_001291"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0856234.1"
                     /translation="MTLFCEQVPYPRLAEALGTPLYVYSQSALTEAFEHYQTAFAALS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IKFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLIEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(10397..10567)
                     /locus_tag="WGR46_001292"
     CDS             complement(10397..10567)
                     /locus_tag="WGR46_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0856235.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSTPQSAPPTQK"
     gene            10638..10961
                     /gene="cyaY"
                     /locus_tag="WGR46_001293"
     CDS             10638..10961
                     /gene="cyaY"
                     /locus_tag="WGR46_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0856236.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDNRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            10988..12004
                     /locus_tag="WGR46_001294"
     CDS             10988..12004
                     /locus_tag="WGR46_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0856237.1"
                     /translation="MNTRPFYFGLIFIAIIAIFANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGTLLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            12030..12449
                     /locus_tag="WGR46_001295"
     CDS             12030..12449
                     /locus_tag="WGR46_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0856238.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALSINDYIHAVYDFVEQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            12471..12977
                     /gene="luxS"
                     /locus_tag="WGR46_001296"
     CDS             12471..12977
                     /gene="luxS"
                     /locus_tag="WGR46_001296"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0856239.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            13123..15918
                     /gene="polA"
                     /locus_tag="WGR46_001297"
     CDS             13123..15918
                     /gene="polA"
                     /locus_tag="WGR46_001297"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0856240.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         3838 a         4336 c         4072 g         3745 t
ORIGIN      
        1 ctgcagatgc ccaacggtct ttatagtgga ttaacaaaaa tcaggacaag gcgacggagc
       61 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttgagcacc ttagagaatc
      121 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataccga
      181 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
      241 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      301 gccgcttgtc gctgcccttg ccgcctgtgc cggggggggg gtagccgaac cgcacgtccc
      361 cgtgtccatc cccaccgcca cgccgttgcc caaaggcgag gtaacgttat caagcgataa
      421 cggcaaaatc gaaaacatca acaccaccag caccggaagc acatccgtca tttccattca
      481 agagcgggcg gttacaaaaa actatttcgg tgttgaatca caagaaaaat cattcatctt
      541 caaaacaccc ggcggcgcgc aatacaccct ttcatcctac gccgacccca taaccgtcag
      601 ctactcctcc cccgatttca aaattcccga ccaccacgca gggcagcggc ttgccgacgg
      661 cagccgcatc tttatctgct gcagcgactc cggggctact tcgtatgcgg aaattaccaa
      721 acaagattat atgaaattcg gggcgtggat aggtcccaac ggcgaaatcg acctcttcgc
      781 cggcggcttc cccgtcggca aaacgccacc tcctgcgttc tcctacggca gcagcacacc
      841 cgaaaccaaa ggcacaggca aaatcactta tcaggtttgg ggcatccgcg tcagaaacgg
      901 gcaatttgtt acttcctctt atacgccgcc gaaaaacagt ggttatacgt tcaatcctac
      961 caacaccccc gtcctttcct ttatcaccgc caatttcaac agtaacaaac tggcaggcaa
     1021 aatcatcggc aacagcgatt acgggccgga tgtggaaatt aaagaagcac agattgacgg
     1081 attatccttt tccggcgatg ccacctccgg cggcaaaacc ggcaaattgg aaggcaagtt
     1141 ttttggcaaa ttcaacagca gctatgacag cgacaccagc ataggcggca aaatcacctt
     1201 tgacggcgac cgctccctcg ataccgtatt cggcggcgtg agttataaaa aagaacttga
     1261 aagcaccacc gatcgagaga ccacccatct cacaaaataa taattaatcc gcttttaaaa
     1321 aggttttctt atgcacatcc ccttcaagcc cgtattggct gccgccgcca tcgcccaagc
     1381 gtttcccgcc tttgcggcag accccgcgcc gcagtccacc caaacgctga acgaaatcac
     1441 cgttaccggc acgcacaaaa cccaaaaact cggcgaagaa aaaatccgcc gcaaaacctt
     1501 agacaagctc ttggtcaacg acgaacacga cctggtgcgc tacgaccccg gcatttccgt
     1561 cgtcgaaggc ggcagggcgg gttctaacgg ctttaccata cgcggcgtgg acaaagaccg
     1621 cgtcgccatc aacgttgacg ggctggcgca ggcggaaagc cgctcttccg aagccttcca
     1681 agaattgttc ggcgcgtacg gcaacttcaa cgccaaccgc aacacttccg agccggaaaa
     1741 cttttccgaa gttaccatca ccaaaggcgc ggactcgctc aaatccggca gcggcgcatt
     1801 gggcggcgca gtcaattacc aaaccaaatc cgcaagcgat tatgtttccg aagacaagcc
     1861 ctaccatttg gggataaagg gcggcagcgt cggcaaaaac agccaaaaat tcagcagcat
     1921 caccgccgcc ggcaggctct ttggtttgga tgccttattg gtttataccc gccgcttcgg
     1981 caaagaaacc aaaaaccgct cgaccgaggg caatgtcgaa attaaaaaca aagggtatgt
     2041 ttacgacccg aacaaaccat tcaacccgag tctgggcccc tcctcctacg tagccacagg
     2101 ggttgcgcgc tcccaacccg acccgcaaga atgggtaaac aaaagcaccc tgttcaagct
     2161 gggctacaat ttcaacgacc gcaaccgcat cggctggatt tttgaagact cccgcaccga
     2221 ccgttttacc aacgagctgt ctaatttgtg gacgagtctc acgggagaca taacgggcga
     2281 ctaccgccac cgccaagacg tgagctaccg ccgccgctcc ggcgtcgaat acaaaaacga
     2341 attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat
     2401 gaacacctgg acttgggaca tcccgaaaga ttacgataca aacggcatca acggccaggt
     2461 ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa
     2521 acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttgggcg gcggcaaagg
     2581 ggacaatgcc aactcggatt acagctattt cgcaaaactg tacgacccca aaatcctcac
     2641 taccaaccaa ggcaaaatca caatgctgat cgaaaaccgg tcgaaatata aatttgccta
     2701 ttggaacaat gcgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg
     2761 ctacgacaaa aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg ccatccgggg
     2821 acagattccc catttgggtt cggaacgcgc gcacgccggc ttcagctacg gcacggggtt
     2881 cgactggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc
     2941 gccgacttcg gacgaaacct ggctgctgtt cccacacccc gatttctacc tcaaagccaa
     3001 tcccaacctg aaagccgaaa aagccaaaaa ctgggaattg ggtctggcgg gcagcggcaa
     3061 agcgggcaac ttcaagctct cgggcttcaa aaccaaatac cgcgacttta tcgaattgac
     3121 gtatatgggc gtttcgtcag acaaccctaa caagcccacc tacgccccgc tttcagacgg
     3181 tacggcattg gtcagctcac ccgtttggca aaaccaaaac cgcaccgccg cctgggtgaa
     3241 aggcatagag tttaacggca cttggaacct cgacagcatc gggctgccca aagggctgca
     3301 caccggcctc aacgtcagct acatcaaggg cagggcaacg caaaacaacg gcaaagaaac
     3361 gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc
     3421 caaacgctgg ggcatcaacg cctacgccac gcgcaccgcc gccaaaaagc cgtccgacac
     3481 cgtccacagc aacgacgact tgaacaaccc gtggccttat gccaaacaca gcaaggccta
     3541 tacgctgttc gacctttccg cctacctcaa catcggcaaa caggtaacgc tccgcgccgc
     3601 cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca gcatccgcga
     3661 gttcggcacg gtcaaccgcg ttgacaacaa aacccacgcc ggcatccaac gctttacctc
     3721 gccgggcagg agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat
     3781 gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc
     3841 ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg
     3901 tcattcccgc gactctccgt cattcccacg gaagtgggaa tctagaaacg caaagctgca
     3961 agaatttatc ggaaatgact gaaactcaac gaacctggat tcccgcctgc gcgggaatga
     4021 cgaagctatc cgcacagaaa cctgcaccac gtcattccca cgaaagtggg aatccagaac
     4081 gtaaaatctg aagaaaccgt tttatccgat acgtttccgc accgacagac ctagattccc
     4141 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
     4201 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaaca acaatgcaaa
     4261 gaacggtgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaccgc
     4321 acggcgttga ccgtgcggct tgttgtctga aggcttcaga cggcattgct tacatcatgc
     4381 cacccatacc gcccataccg cccatgtcag gcatagcagg tttttcttca gggatttcgg
     4441 caatcataca atcagtggtc agcatcaggc cggcgataga tgcggcgtgt tgcagcgcgg
     4501 aacgggttac tttggcgggg tcgagtacgc ccatttcgat catgtcgccg tattcgccgg
     4561 agcctgcgtt gtaaccgtag ttgcctttgc cttccaacac tttgttcacc accacgctag
     4621 gttcgccgcc tgcgttggca acgatttggc gcagcggaga ctcaacggcg cgcaagacga
     4681 tttgtacgcc tgcgtcttgg tcggcattgc cggtgtgcag gttttccaga gcggcacggg
     4741 cgcgcaacag ggcgacaccg ccgcctgcaa ccacgccttc ttcaacggct gcgcgggtag
     4801 cgtgcagcgc gtcttccacg cggtcttttt tctctttcat ttcgacttcg gtcgcggcac
     4861 cgactttgat cactgccacg cctcctgcca atttggcaac gcgctcttgc agtttttctt
     4921 tgtcgtaatc gctggtcgcg gtttcgattt gttggcggat ttcggcaaca cgcgcttcga
     4981 tttgggctgc gtcgccgaag ccgtcgatga tggtggtgtt ttctttaccg atttcgatgc
     5041 gtttggcttg acccaagtcg tccaaagtcg ctttttccag agacaggccg acttcttcgg
     5101 aaatcaccac gccgccggtc aggatggcga tgtcttgcaa catcgctttg cggcggtcgc
     5161 cgaagccagg ggctttgacg gcaacggttt tcaggatgcc tcggatgttg ttcacgacca
     5221 aagtcgccaa ggcttcgcct tctacgtctt cagcgataat caacagcgga cggctggctt
     5281 ttgccacttg ttccaaaaca ggcagcaagt cgcggatgtt gctgattttt ttgtcgaaca
     5341 acaatacaaa cggattgtcc aaagcagcga tttgtttttc agcgtcgttg atgaagtaag
     5401 gagacaagta gccgcggtcg aactgcatac cttcaaccac gtccagctcg ttttccaaag
     5461 atttgccgtc ttcaacggta atcacgcctt ctttgccgac tttttccatc gcttcggcga
     5521 taatcgcgcc gacttgttcg tcagagttgg cggaaatcga gccgacttgg gcgatttctt
     5581 tagaagtgtc gcaaggtttg gcgatgtttt tcagctcgtc aaccaaagcg gcaacggctt
     5641 tgtcgatacc gcgtttcagg tcggtcgggt tcataccggc ggtaacgtat ttcatacctt
     5701 cggcaacgat ggattgcgcc aatacggtgg cggtagtcgt accgtcgccc gccacgtcgt
     5761 tggttttgga cgcgacttct ttcaccattt gcgcgcccat attttcaaac ttgtctttca
     5821 attcgatttc tttggcgacg gttacgccgt ctttggtgat gtgcgggccg ccgaatgcgc
     5881 ggtcaaccac cacgttgcgg cctttgggac ccaaggttac gcgtacggcg tttgccagaa
     5941 tgttcacgcc gtttaccatt ttttgacgga cttcattgcc gaactgtacg tcttttgctg
     6001 ccatttcaat tctccaaaaa tcattaaaac tgtctgataa aaccgtttat gccgtctgaa
     6061 ggcggtttgc cgtttcagac ggcatcgtgt ccgtatttat ttttcaacga tgccgaaaat
     6121 atcttcttcg cgcattacca acagctcttc gccgtcggct tttacggttt ggccgctgta
     6181 tttgccgaag atgattttgt cgccgacttt gacatccagc ggacggcgcg cgccgtcttt
     6241 accgattttg cccgcgccca cggcgatgac ttcgcccatg tcgggttttt cggcggccgc
     6301 acccggcaaa acgatgcccg atgcggtttt ttcttcagct tccaagcgtt tgacgacaac
     6361 gcggtcgtgt aaaggacgga tggtcatatt tatgctccga taaaatgatt tgaaaacaat
     6421 catctgcccg aacggttcgg acagattgaa gtggaaaccg ggctgccgcc tgccggcccg
     6481 cccgtataag tcggcaaaat tagggtgtgt gcgggcaaat tcaagtgagg cggaaaaaat
     6541 ttatttccgc cgttttttat agtggactaa atttaagggg ctgtactaga ttagcagata
     6601 tgttaccctc gaaatatgaa gataacgcac tgcaaattaa agaaaaaagt acagaaagaa
     6661 ctgctccgtt tttttgtact cgaagttacc gcccgttctg ccgccgatat tttgggtatc
     6721 catcccaatt cggcagcact gttctaccgt aaaatccgca cggttatcaa ccatcatttg
     6781 gccctggctg ccgatgaggt ttttgaggat cctgtcgagc cggacgaaag cgatttcggc
     6841 ggacggcgta aaggcagacg tggtcgcggt gcggcaggaa aagtggttgt cttcggcatt
     6901 ctgaaacgca acggacgggt ctataccgtt gtggtggata atgccaagtc tgaaacgtta
     6961 ctccctgtca tcaaaaagaa aatcatgccg gacagtattg tttataccga tagtctgagc
     7021 agctgcgaca agttggacgt gagcggtttt atccattacc gcatcaacca ttccaaggaa
     7081 tttgcagacc gtcagaacca cattaacggc attgagaatt tttggaatca ggcaaaacgc
     7141 gtcttgcgaa aatacaacgg aatcgattgc aaatctttcc cgctgttctt gaaagaatgc
     7201 gaatttcgat ttaacttcgg cacaccgtct caacagctta aaatcctgcg ggattggtgt
     7261 gggatttagg gctaatctag tacagcccct aaaatttttc gttttcaagc cttcaccgct
     7321 tgccatcagc gttaaatttt tttacgataa gcacatagat tgtaaacaat cggccacaag
     7381 ccggtttgtt ttttcagaag acattatccc tgtcagacgc tgtttctata tatttcgcct
     7441 ataatggctt gtttttaata aataattcaa gaggtatcaa cgtgtctgat tccaagacga
     7501 aagaacgcgc cacattcggc acgcgccgcg cgtttatgat tgccgccatc gggtccgccg
     7561 tcggcttggg caatatttgg cgtttcccct atattgcttt tgaaaacggc ggcggcgcgt
     7621 tcatcctgcc ctatctggtc gcgcttctga cggcgggcat cccgctgctg ctgctcgatt
     7681 atgccatcgg ccaccgttac cgtggttctg cgcccttggc tttccgccgc ctcggacgat
     7741 ggtttgagcc ggtcggctgg tggaacgtga tgaccaatat cgtcatctgc atctattacg
     7801 cggtaattat cggttgggcg gcaagctata cctattattc ggtcaacgcc gcctggggtg
     7861 cggatccgca gggttttttc tttaaggact tcctgcaaat ggcgggcccg gaagccttgg
     7921 gtttggattt tgtcggcaaa gtcgccggtc ctttggcggg cgtgtgggtt tttaccgccg
     7981 ccattatggc gttgggcgtg caaaagggcg tggcgcgcgc ctcgtcgttc tttatgccgc
     8041 tgcttttggt gatgtttttg attatggtcg gcatttcact aaccctgccg ggtgcggcaa
     8101 agggcttgga cgcattgttt acgcccgact ggtcgaaact cgccgattcc aaggtctggg
     8161 tggcggcata cgggcagatt ttcttttcgc tttccatctg cttcggcatt atggttacct
     8221 attcttctta tttgaagaaa aaaaccgact tgggcggaac ggggctggtg gtcggttttg
     8281 ccaacagcag ctttgaactg ctcgcgggca tcggcgtgtt tgccgcattg ggctttatgg
     8341 cgcaggcggg cggtaaggcg gtcaacgagg ttgcctcagg cggcatcggt ttggcgttta
     8401 tcgcctttcc gaccattatc aaccaggcac cgatgggctg gctgatcggc atattgtttt
     8461 tcggttcgct ggtgttcgcc ggcgttacgt cgatgatttc catccttgaa gtgattgtgg
     8521 cggcgattca ggacaagctg aacatcgggc gcgtcaacgc cacgctgctg gtctgcattc
     8581 cgatgggcat tgtttccacg ctgctgttcg gtacggcgac ggggctgccg gttttggacg
     8641 tgatggacaa attcgtcaac acctacggca ttgttgccgc cggctttgtt tatgttgccg
     8701 ccatcatcat cagcggcagg ctgccggaat tacgcaagca cctgaacgct ttgtcctcca
     8761 tccgcatcgg cggcttgtgg acggtctgcg tcgtggttac cgtcgtgatg ctcggctata
     8821 tgctgtttaa agataccagc ggcctgatgg agaaaaatta cgaaggttat ccggatggtt
     8881 tcctcagtat tttcggctgg gggatgtcgg cggcgttggt cgtgttcggg ctgctgctgt
     8941 cgttgctgcc ttggaaacac ggtcaggatt tcaacgtcaa agacgaacac gaacatgaac
     9001 aaggagaaga aaaatgagta cttccgccat tgtgatgatg attgtctcaa tcgtgataat
     9061 ctggggaggg ctgctgcttt ccctgttaag gctgccgaac gagtaagcct ttagagcgtt
     9121 aaaaatgccg tatgaaccgc ttcagacggc attttgatgg tcggcttaca ggcaggcgag
     9181 ttcgtttgcc atttgctgtt ccaaggtttc gcgccggcgg atgagtcggt attcgttgcc
     9241 gtccaccaac acctctgccg cacggttgcg cgcgttgtaa ttgctcgcca tactggctcc
     9301 gtatgcgccc gcgctgcgga taagcagcaa atcgccttct tcgcaggcga tggtgcggtc
     9361 tttgccgagg aagtcgccgg tttcacaaat cgggccgacg atgttggcgg tcagcggcgc
     9421 gatgtttttt ggttctaccg cttcgatgtg gtggtaggca tcgtatagtg cggggcgcat
     9481 caaatcgttc atcgccgcat cgaccatcac aaagtttttc tcttcgccgt gtttgacgaa
     9541 ttcgacacgt gtcaataacg cgcctgcgtt gccgaccaag ctgcggccgg gctcaaggat
     9601 gagtttcaga cggcgtgtcc ccatcagttt ttgaaccgcc cgggcatacg cgcccaagtc
     9661 gggcacattt tcgtcttggt aaacgatgcc gacgccgccg cccaagtcca aatgttccaa
     9721 aacaatgcct tcggcggcaa gagcgtcaac caaaatcaaa atgcgttcgc aggcttcgat
     9781 gagcgggctt aagtcggtca gttgcgagcc gatgtggcag tcgatgccga tgattttcaa
     9841 attgggctgt tgtgcggcat ggcggtaggc ttcgagcgcg tcggcgtagg cgatgccgaa
     9901 tttgttggct ttcagtcctg tggagatgta gggatgggtt ttcgcatcga catcggggtt
     9961 gacgcgcagg gagacgggcg cggttttacc caaacgcgcg gcaacttttt gaatgcggtc
    10021 gatttcgggg atgctttcca tattgaagca ttttacgccg gcattcagcg cgaacttgat
    10081 ttccgcctcg cttttgccta cgcctgaaaa tatggttttt gctgcatcgc cgcctgccgc
    10141 caaaacgcgt gccaattcgc cgccggacac aatgtcaaaa ccgctgccca gcgaggcgaa
    10201 gtgtttgatg atgctcagat tgccattggc tttgacggca tagcagacga gtggggaaag
    10261 cgcggcaaag gcggtttggt agtgttcaaa cgcttcggtc agcgcggatt ggctgtacac
    10321 ataaagcggc gtgccgagtg cttcggcaag gcgggggtag gggacttgtt cgcaaaacag
    10381 ggtcatattt tcgttttcat ttttgggttg gtggggcgga ttgcggtgtg ctttgaagct
    10441 gcaaaccggt ttggattacg ccgaaacgcg ccttgtcgcc ttctttgggc aggtagaggt
    10501 cgcctttgta accgcaggcg gaaagcagga gggcggttgc cgccgcaaaa aatacgccgt
    10561 atttcatcgg taaacttcct tcataagcgc gaatgtggca agattcggca tcttaaacaa
    10621 aaaacacgca aaaagccatg atgaccgaaa gcgagtttat ccgcgcgagc gaagcattat
    10681 ttgaacacat cgaagaccaa atcgacgaaa acggctggga tttcgactgc cggtttgccg
    10741 gaaacgtcct gaccatcgaa gccgaaaacg gcacgcaaat catcgtcaac cgccacacgc
    10801 ccaaccaaga attatggatt gccgccaaaa gcggcggcta ccatttcgcc gaacaaaacg
    10861 gcaaatggct ggcaacgcgc gacaaccgcg atttttacga cgttttaaac gaagccctga
    10921 gcgcggcttc gggcgaagcg gtaaagattg ccgaattgta atttgagtgt tatcacggag
    10981 agaaaaaatg aacacacgtc ccttttattt cggacttata tttatcgcga ttatcgctat
    11041 atttgctaac tatttaggaa acactgattt ttcccatcat tatcatatca gtgctttaat
    11101 tattgctatc ttgctgggaa tggcaatcgg caataccatt tatccgcaat tttcaacaca
    11161 agtggaaaaa ggcgttttgt ttgccaaagg cacgcttctt cgcactggca ttgtgctgta
    11221 tggtttccgc ctcacttttg gcgatattgc cgatgtagga ttaaatgcgg ttgtcactga
    11281 tgcaatcatg ctaatttcaa ccttcttttt taccgtactt ttaggcattc gttatctaaa
    11341 aatggataaa caattggttt atctcactgg ggcaggttgc agcatttgcg gtgcggcagc
    11401 ggttatggcg gcagagtctg ttaccaaagc agaatcccat aaagtttcag tggcgattgc
    11461 catagtggtc attttcggga cgcttgctat ttttacttac cccttgttct acacgtggtc
    11521 acaagattta attaacgccc atcaattcgg tatttatgtt ggttctagtg tacacgaagt
    11581 ggctcaagtg tatgcgattg gggaaaatat tgatcctatc gtggcgaata ctgccgtcat
    11641 ttccaaaatg atccgagtga tgatgctcgc ccccttttta ttaatgcttt cttggttatt
    11701 aacacgtagt gatggggtat cagaaaatac ctcacacaaa attacaattc cttggtttgc
    11761 tgtacttttt attggtgttg ccatttttaa ttcttttgat ttattaccaa aagaactcgt
    11821 gaaattattc gttgaaatcg attctttctt attaatttca tcaatggctg cgcttggctt
    11881 aacgacgcac gcaagcgcaa tcaaaaaggc aggattaaaa ccgtttgttt taggaatact
    11941 aacttattta tggctagtgg ttggtggatt tttagtgaac tatggaatat caaaattaat
    12001 ataaaattca ctaaagagag cgttacccaa tggcacaatt accgctatat ctgacttctg
    12061 aaatcaaaga ctttactgtc ggcacgccta aagttttgga atcattttcc aaacatatcc
    12121 cttatggtgt tgtctttgaa gacgacggcg acacgggcta cttctatgcc gcttcgcaag
    12181 acgggattct agatgccttg cacatctaca atgtcgaaga tgtatccgac aaacatatcc
    12241 ccaatcatgt cttgatttta tgggatgatg cctgcaccat agccgcattg agtatcaacg
    12301 actacattca tgccgtctat gattttgtcg aacaggcagg atattgccgc aacggcttcc
    12361 ctgaaacagg cggcgaatgg gtgaaagtcg aaaaccgcgt cttggacgat gaattgctgg
    12421 acaaaatctt atcccgaaaa tctacataac ctactcaaaa ggataacaaa atgcccctgt
    12481 tagacagttt caaagtcgac cacacccgta tgcacgcccc tgccgtacgc gtggcgaaaa
    12541 ccatgaccac gcccaaaggc gataccatta ccgtattcga cctgcgcttt tgcgttccta
    12601 acaaagaaat cctgcctgaa aaaggcatac acacgctgga gcatttgttc gcaggcttta
    12661 tgcgcgacca cttaaacggc aacggcgtcg aaatcatcga catttccccg atgggctgcc
    12721 gcaccggttt ttatatgagc cttatcggca cgccttccga acagcaggtc gccgatgcgt
    12781 ggctggcttc gatgcaggat gttttgaatg tcaaagacca aagcaaaatc cccgagttga
    12841 acgaatacca atgcggcact tatcaaatgc actcgctcgc cgaagcgcaa caaatcgcgc
    12901 aaaacgtgtt ggcgcgcaaa gtggcggtga acaaaaatga agagctgacg ctggatgaag
    12961 ggctgctgaa cgcctaatcc gccaaaaatg ccgtctgaac aagggtttca gacggcattt
    13021 gccttttccg ttataatctg gggttgtgcg ggggcgggtt ttaagccggc atcgtccttc
    13081 cctatttttt tctgtccctt atcggtttta agcgggtttt ttatgtccaa cagacctaca
    13141 ctcctcctcg ttgacggatc gtcctacctc taccgtgcgt atcacgcgat ggggcaaaac
    13201 ctgaccgcac ccgacggtgc accgacgggt gcgctgtatg gcgtattgaa tatgttgcgc
    13261 cgtttgcggt cggaatatcc gcacgattat tgcgcggtgg tttttgatgc gaaaggcaaa
    13321 aatttccgcc atcaaatgtt tgaagaatac aaggcgacgc gcccgccgat gcccgacgat
    13381 ttgcgtccgc aggcggaagc tttgccggat ttggtgcgcc tgacgggctg gccggtattg
    13441 gtgattgggc aggtggaagc ggacgatgtg atcggcacgc tggcaaaaca gggggcggaa
    13501 catggtttgc gagtcattgt ttcgaccggc gataaggaca tggcgcagtt ggtggatgag
    13561 cgcgttacgc tggtgaacac gatgagcgga gaaacgctgg atattgaagg cgtgaaggca
    13621 aaattcggcg tgcgccccga ccaaatccgc gattatctcg cgctgatggg cgacaaggtg
    13681 gacaacgtgc cgggcgtgga aaaatgtggc ccgaaaacgg cagtgaagtg gctggaagcc
    13741 tacggctcgt tgcaaggcgt gatagaacac gcttcggaaa tcaagggcaa agtgggcgag
    13801 aatctgcgtg ccgccctgcc gcgcctgccg ctgtcgtatg atttggtaac gattaaaacc
    13861 gatgtggact tgcacgccga gctttcagac ggcctcgaaa gcctgcgccg taccgcgccg
    13921 aaatgggcgc agctggttgt cgatttcaaa cgctggggct tccgcacctg gctgaaggaa
    13981 gcggaaagcc gtatgcacga agcggcggac ggcgatttgt tcggcagcga tacgataggc
    14041 gaacaggcgg cgttggatat ggaaacgtcg tctgaaaggc tacctgaaaa agccgttgcc
    14101 cccgaaaagc tggattatca ggccgttacc accgaagcgc agtttgccac tttgttagac
    14161 aaattgtcgc aggcggaaaa aatcggcatc gacacggaaa ccacttccct agacgcgatg
    14221 aacgccgcgc tggtcggcat cagcatcgcg ttccaagcgg gcgaagcagt ttacatcccc
    14281 gtaggacaca gcctgaccgc cgcgccagaa cagcttgatt tgcaagacgt attaggccat
    14341 ctgaaaccgt atttggaaaa tcctgccctg aaaaaaatcg ggcaaaacct caaatacgac
    14401 caacacgttt tcgccaatta cggcatcgcc ctgaacggca tcgccggcga cgccatgctc
    14461 gcttcctaca tcatcgagag ccatctcgga cacggcttgg acgaattgtc cgaacgctgg
    14521 ctcggcttgg aaaccattac ctacgaatcg ctgtgcggca aaggcgcgaa gcaaatcggt
    14581 tttgccgatg tcgccatcgg gcaggcgacc gaatacgccg cccaagacgc cgatttcgcc
    14641 ctgcgcctcg aagcgcacct gcgcgcgcaa atggacgaaa aacagcttga aatgtatgaa
    14701 aaaatggagc tgcccgtcgc gcaggtattg tttgaaatgg aacgcaacgg tgtgcaaatc
    14761 gaccgcgccg aactcgcccg ccaaagcgcg gaactcggcg ccgagctgat gaagctcgaa
    14821 caagaagcct acgccgccgc agggcagccg ttcaacctca actcgcccaa acagctgcaa
    14881 gaaatcctgt tcgacaaaat gggcatcccg acaaaagggc tgaaaaaaac cgccaagggc
    14941 ggcatttcca ccaacgaggc cgtgctcgaa cagctcgcgc ccgactaccc cttgcccaaa
    15001 atcatcctgc aaaaccgcag cctggcgaaa ctcaaatcca cctacaccga caaactgccc
    15061 gacatgattt ctccccggga taaccgcgtg cataccacct acgcccaagc cgtcgccatt
    15121 acaggacgct tggcaagcaa caaccccaac ctgcaaaaca tccccatccg caccgccgaa
    15181 ggccgccgcg tgcgccgcgc ctttaccgcg ccaccgggca gcgtcatcgt ttccgccgac
    15241 tattcccaaa tcgaactgcg catcatggcg cacctctctg gagacaaaac cctcattgcc
    15301 gcattccaaa acggcgaaga cgtacaccgc cgcaccgccg ccgaagtgtt cggcactgcg
    15361 cccgaaaacg tctcgcctga gcaacgccgc tacgccaaaa ccatcaactt cggcttaatc
    15421 tacggtatgg ggcaatacgg ccttgccaaa tcattgggca tcgacaacct ttccgccaaa
    15481 aactttatcg accgctactt cgcccgctac cccggcgtcg ccgaatacat gcagcgcacc
    15541 aaagaacaag ccgccgccca aggcttcgtc gaaaccctgt ttggccgccg cctctacctg
    15601 cccgacatcc gcaacaaaaa cgccaacgcc cgcgccggag ccgaacgcgc cgccatcaac
    15661 gcccccatgc aaggcaccgc ctccgacctt atcaaacgcg ccatgataga cgtgtcccgc
    15721 tggctggttt cagacgacct gaagagcaaa ctgattatgc aggtgcatga cgaactggtg
    15781 ctggaagtcg ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg
    15841 aaagtgggcg gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat
    15901 tgggaagagg cacattgatt gaaaggtgtt attatgctat ctttatttaa ataaaattta
    15961 atttttggta tattttttct aaatgttcct a
//
LOCUS       DAWFKN010000043        15972 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFKN010000043 DAWFKN010000000
VERSION     DAWFKN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.043"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            79..600
                     /locus_tag="WGR46_001298"
     CDS             79..600
                     /locus_tag="WGR46_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856241.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGSYEGYWREKSDKKEGMIVVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCRQTAQAYLDAQKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPRALLL"
     gene            complement(851..913)
                     /locus_tag="WGR46_001299"
                     /pseudo
     CDS             complement(851..913)
                     /locus_tag="WGR46_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1019..2344)
                     /locus_tag="WGR46_001300"
     CDS             complement(1019..2344)
                     /locus_tag="WGR46_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0856242.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDQHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            2557..3396
                     /locus_tag="WGR46_001301"
     CDS             2557..3396
                     /locus_tag="WGR46_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0856243.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            3393..4559
                     /locus_tag="WGR46_001302"
     CDS             3393..4559
                     /locus_tag="WGR46_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0856244.1"
                     /translation="MRKFNLTTLSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            4703..5968
                     /gene="efeB"
                     /locus_tag="WGR46_001303"
     CDS             4703..5968
                     /gene="efeB"
                     /locus_tag="WGR46_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0856245.1"
                     /translation="MSTNNPTQPTRRTLFKTALAAGVGGAIGGYLGGKGLGETAERTA
                     ESQHSPQAYPCYGKNQAGIVTPQQAFSIMCAFDVTAQNAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(6040..6368)
                     /locus_tag="WGR46_001304"
                     /pseudo
     CDS             complement(6040..6368)
                     /locus_tag="WGR46_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
     gene            complement(6444..7814)
                     /gene="glmU"
                     /locus_tag="WGR46_001305"
     CDS             complement(6444..7814)
                     /gene="glmU"
                     /locus_tag="WGR46_001305"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0856246.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7869..8141)
                     /locus_tag="WGR46_001306"
     CDS             complement(7869..8141)
                     /locus_tag="WGR46_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0856247.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(8134..8847)
                     /locus_tag="WGR46_001307"
     CDS             complement(8134..8847)
                     /locus_tag="WGR46_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0856248.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            8906..9907
                     /locus_tag="WGR46_001308"
     CDS             8906..9907
                     /locus_tag="WGR46_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856249.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            10001..10849
                     /locus_tag="WGR46_001309"
     CDS             10001..10849
                     /locus_tag="WGR46_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0856250.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10875..11360
                     /locus_tag="WGR46_001310"
                     /pseudo
     CDS             10875..11360
                     /locus_tag="WGR46_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
     gene            complement(11462..13030)
                     /gene="msrAB"
                     /locus_tag="WGR46_001311"
     CDS             complement(11462..13030)
                     /gene="msrAB"
                     /locus_tag="WGR46_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0856251.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            13176..14441
                     /gene="ftsY"
                     /locus_tag="WGR46_001312"
     CDS             13176..14441
                     /gene="ftsY"
                     /locus_tag="WGR46_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0856252.1"
                     /translation="MFSFFRRKKKQETPAPEEAQIQETAAKAESETAQVIENIKEDVN
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            15030..15314
                     /locus_tag="WGR46_001313"
     CDS             15030..15314
                     /locus_tag="WGR46_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0856253.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT         3574 a         3960 c         4699 g         3739 t
ORIGIN      
        1 tttttgcaaa tcaatattga caagctgaaa aaaaaaaaca atatactcac ccagtcttaa
       61 tgttaacgga gtatggaaat gaaacaaatg cttttggccg tcggcgtggc ggcggtgttg
      121 gcgggctgcg gcaaggatgc cggtagttac gagggttatt ggcgcgaaaa atcggacaaa
      181 aaagagggta tgattgtcgt caaaaaagaa aaaggcaatt acttccttaa taaaatcaac
      241 gtggttacag gtaaggaaga atctatgctt ttgtctgaaa aagacggcga gctttcgata
      301 aacacgggga taggggaaat cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc
      361 gaacgcaggc agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat cgcccaccag
      421 aaaaagtgca gacaaacggc acaggcatac ctcgacgcgc aaaaggcgtt gccgtcaaac
      481 caaacgtatc agcagcgtca ggcggcgatc gagcaattga aacggcggtt tgaagccgag
      541 ttcgatgaat tggaaaaaga aatcaaatgc aacggcagac cccgggcatt gttgctttag
      601 cggaggataa ccgggaggat gccgccgtcc gaatcggacg tgcggtttcc gtaccggtac
      661 gggcgggcag gaatgtccgc ctttttttgt tcggacgcgt ttgaataccc gtttgattcc
      721 gaccgtttgc aaggggtatt tccgttcggg cggaaattat agtggattaa caaaaaccag
      781 tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctc aagcaccaag
      841 tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat
      901 ttgatccact ataattccgt caaataagaa aggaattttg tgcctgcggt atcgcaaaac
      961 ttagccttaa tgcgcccgat tgcctaggga tgggcttcag atggcattgt tttccggttt
     1021 acgggcggta ttcgggcttc ataccgttgg gtaggagctg ccagacgtaa cccgtggttt
     1081 tctgtttgcc ggcaagttcg ccggcttcgt cgccgtatcc ccaaaaataa tccacgcgca
     1141 ccgcgccttt aatcgcgctg ccggtatcct gcgccataat caggcggttg agggctttgc
     1201 gggtaaccgg atgggcggtg gcgacaaata agggcgcacc caaggtaatg tagtgctggt
     1261 cgactgcgcc ggcatattcc cccatcagcg gcgtgcccag tgcgccgaca gggccgtcat
     1321 tgccgcttcc ggtaagctcg cggaaaaaga tatagctggg gttttgaccc aaaacttcgg
     1381 cgaggcgttg cggattttgc cgcatataag acttaatgcc ctgcatggag gtttgtccga
     1441 gtttgaggta gcccttatcc gccatgtatt ttccgatgga aacataggga tgctcgtttt
     1501 tgtcggcata gccgatgcgg atgtatttgc cggacggggt tttcagacgg cccgagcctt
     1561 ggatgtgcat gaaaaagagt tcgacggggt cttcggcgta accgagtatc ggtgctttgc
     1621 cgtcaagcgc gccgccgttg atttggtttc gcgtgtggta agggaggaag cggcttcctt
     1681 caaacctgcc cttgattgcc gttgtgcgtg cggtgatggg gaatcgggag aggtcggcgg
     1741 tatgtgtgcc gccggtattg tcgattgtgc cgctgttttt tcccgtctgc ctgatgcgga
     1801 caagggcttt tccgctccgc aaaccggcag gcagggggac ggagataaaa tcgtcgggaa
     1861 taccgtaaat cgggaagcgg gcttgtgccg tccgcctgtc gtcgcccttc agcaccggct
     1921 cgtaatagcc ggtaaccgta ccggcaaggc ttccgttgcc tgcaacctgc cacggcgtga
     1981 aatagcgttc aaaaaactgt tttgcctgaa aggaatggac gggggtttga aaggcttggg
     2041 cgcacacatc ctgccagcct tggcggtttt tcaaattggc gcagccgagg cggaaggatt
     2101 gcaggctttt ggcgaaatcc tgcgccgccc agtggggcag ggacaggtgc ggcacaacgg
     2161 tataaacggc cccgccgccg ccgaccgtcg ttccggcggg gtcggggatg ccgaccggcc
     2221 ggtccgggcc gttgatgacg gatgtgtcgg gttgcggaaa ggtttggatg ctcttgcttt
     2281 ggcaggcggc gaggatggcg gcggcgatgc cgcacagggc ggcgcggaac aggtattttt
     2341 tcataatgga caatgttgcc ggcagtaata aaaaaaagat ggtttcgggc ggcgttgcgg
     2401 cagccgtgga gaggggattt taacacaggg cgcagctgca gcctgcggaa ctttccgccg
     2461 cgcggtactg cagataaaaa taacttgcat ttgtatttac aagcaatgaa aatattccga
     2521 taatatatta ttcatcatcc ttgttcgttc gcgtttatgc tggtcgcttt tttaattatg
     2581 ttgcgcgagg gtatcgaagc cgcgctcatc gtcggcatcg ttgccggttt tctgaaacag
     2641 tccggacatt ccaaactgat gcctaaggtc tggttcgggg tcgtccttgc ttctttgatg
     2701 tgtttgggca tcggatacgg catccattcg gcaacgggcg agattcccca gaagcagcag
     2761 gagttcgtcg tcggcattat cggtttggtt gctgtcgcga tgctgactta tatgatttta
     2821 tggatgaaaa aggcggcgcg ttcgatgaag cggcagcttc aggattctgt acaggcggct
     2881 ttgaaccgtg gcagcggtca aggatgggcc ttggtcggta tggcgtttct tgccgtggcg
     2941 cgcgaaggtt tggagagcgt ttttttcctg cttgccgtat tcaaacagag cccgacgtgg
     3001 cagatgccgg ccggtgcggt agcgggggtt ttggctgccg ccgtgattgg cgcgttgatt
     3061 tatcagggtg ggatgcgcct gaatctggcg aagtttttcc gttggacggg ggcgtttttg
     3121 attgtcgttg ccgccggcct gcttgccggc tcgctgcgcg cgctgcacga agcaggcatt
     3181 tggaacgcgc ttcaggacat tgtgttcgac tcatcaaaat atttgcacga agacagtccg
     3241 ttaggtgtgc tgctcggcgg atttttcggc tataccgacc atccgacgca gggcgaggcc
     3301 ttggtttggc tgctgtacct tattcccgtc ataacttggt ttttgtgcgg cagcaggccg
     3361 tctgaaactt taacccgtaa agaggagctg aaatgagaaa attcaatttg accacattgt
     3421 ccgtgatgct tgccttaggt ttgaccgcgt gccagccgcc ggaggcggag aaagctgcgc
     3481 cggccgcgtc cggtgaggcg caaaccgcca acgagggcgg ttcggtcagt atcgccgtca
     3541 acgacaatgc ctgcgaaccg atggaactga ccgtgccgag cggacaggtt gtgttcaata
     3601 ttaaaaacaa cagcggccgc aagctcgaat gggaaatcct gaaaggcgtg atggtggtgg
     3661 acgagcgcga aaacatcgcc cccggacttt ccgataaaat gaccgtcacc ctgttgccgg
     3721 gcgaatacga aatgacttgc ggtcttttga ccaatccgcg cggcaagctg gtggtaaccg
     3781 acagcggctt taaagacacc gccaacgaag cggatttgga aaaactgtcc caaccgctcg
     3841 ccgactataa agcctacgtt caaggcgagg ttaaagagct ggtggcgaaa accaaaacct
     3901 ttaccgaagc cgtcaaagca ggcgacattg aaaaggcgaa atccctgttt gccgacaccc
     3961 gcgtccatta cgaacgcatc gaaccgattg ccgagctttt cagcgaactc gaccccgtca
     4021 tcgatgcgcg tgaagacgac ttcaaagacg gcgcgaaaga tgccggattt accggctttc
     4081 accgtatcga atacgccctt tgggtggaaa aagacgtgtc cggcgtgaag gaaattgcag
     4141 cgaaactgat gaccgatgtc gaagccctgc aaaaagaaat cgacgcattg gcgtttcctc
     4201 cgggcaaggt ggtcggcggc gcgtccgaac tgattgaaga agtggcgggc agtaaaatca
     4261 gcggcgaaga agaccggtac agccacaccg atttgagcga cttccaagcc aatgtggacg
     4321 gttcgaaaaa aatcgtcgat ttgttccgtc cgctgattga ggccaaaaac aaagccttgt
     4381 tggaaaaaac cgataccaac ttcaaacagg tcaacgaaat tctggcgaaa taccgcacca
     4441 aagacggttt tgaaacctac gacaagctga gcgaagccga ccgcaaagcg ttacaggccc
     4501 ctattaacgc gcttgccgaa gaccttgccc aacttcgcgg catactcggc ttgaaataag
     4561 ccttaagcgt tcagacggca tttagcgaca gatgccgtct gaaattttat ctgcaaaacc
     4621 ggaaaggtat tgaaaaaggc ggaggtttcc gtttcccgcg ttccgccgta tcggataccg
     4681 agtttaaata aaggataaaa acatgagtac gaacaacccg acacaaccga ccaggcgcac
     4741 cctctttaaa accgcactcg cagccggcgt gggcggcgca atcggaggtt atctcggtgg
     4801 gaaggggctg ggtgaaactg ccgaacgcac cgccgaaagc caacactcgc cccaagccta
     4861 tccctgttac ggaaagaatc aggcaggtat cgttacgccg cagcaggcat tttccatcat
     4921 gtgcgccttt gacgttaccg cgcaaaacgc caagcagctg gaaaacctgt tccgcacgct
     4981 gactgcccgt atcgagtttc tcactcaagg cggtgaatac caagacggcg acgacaaact
     5041 tccgccggcc ggcagcggca ttttgggcaa agccttcaac cccgacggat tgaccgttac
     5101 cgtgggggtg ggcagcagcc tgtttgacgg acgattcgga ctcaaagaca aaaaaccgat
     5161 tcatttgcag gaaatgcgcg acttctccaa cgataagctg caaaaaagct ggtgcgacgg
     5221 cgatttgagc ctacaaatct gtgcctttac ccccgaaacc tgccaagccg ccctgcgcga
     5281 catcatcaaa cacaccgtcc aaaccgccgt tatccgttgg agtatcgacg ggtggcagcc
     5341 caaatccgaa cccggcgcga tggcggcgcg caacctgttg ggcttcaggg acggcacggg
     5401 caaccccaaa gtttccgacc ccaaaaccgc cgacgaggtt ttgtggacgg gtgtggccgc
     5461 caacagcctc gacgaaccgg agtgggcgaa aaacggcagc tatcaggcag tccgccttat
     5521 ccgccacttt gtcgagtttt gggacaggac gccgcttcaa gagcaaaccg acattttcgg
     5581 gcggcgcaaa tacagcggtg cgccgatgga cggcaaaaaa gaagccgacc aaccggattt
     5641 tgccaaagac cccgagggga ataccacgcc caaagacagc catatacgcc tggcgaatcc
     5701 gcgcgatccc gaattcctca aaaaacaccg cctcttccgc cgcgcctaca gctattcgcg
     5761 cggactcgcc tcaagcggac agcttgatgt cggactggtg ttcgtctgct atcaggcaaa
     5821 ccttgccgac ggattcatct tcgtgcaaaa cctcctcaac ggcgaaccgc tggaagaata
     5881 catcagcccc ttcggcggcg gctatttctt cgtcttgccc ggcgtggaaa aaggcggctt
     5941 tttggggcaa gggctgctgg gcgtataaat ccgccatata aaaaacgccg tccgaacctt
     6001 gccaaacggg ttcggacggc gtttcttgtt tttgggcggt caggcttttt tgacgaattc
     6061 ggattttaaa ttcatcgcgc tgccgtcgat tttgcagccg atgtgtgatc gccttcctgc
     6121 aggcgtatgc ctttgacttt tgtaccttgt ttgatcacca tcgagctgcc ttttaccttg
     6181 aggtctttga tgaggatgac ggtatcgccg ttttgcagtg ccgcgccgtt ggcatcgcgc
     6241 acttgagccg caaggtcggc ggcggattcg gtttcattcc attcgtgggc gcattcgggg
     6301 cagatgtatt gtccgccgtc ttcataggtg tattcggagg cgcattgcgg gcatgggggt
     6361 aatgacatgg ttttccgtcc ttatcggatg tttgtttttg ggatgccgtc tgaaacctga
     6421 aaccggcttc agacggcatg gctttattgt ttgtcttttt ccggcctcac ccatccttcg
     6481 atgacggttt ggcgggcgcg ggcgagggcg agtttgttgt cttcgatatt gcgggtaatc
     6541 gtgctgcctg cgcctgtggt tactttgttg ccgagggtaa cgggggcgac taggacgcag
     6601 tttgaaccga tgcggacttc gtcgccgata acggttttgt gtttgtgcac gccgtcgtag
     6661 ttggcaataa tcgtaccggc gccgaagttg gttttgctgc cgacttcggc atcgccgatg
     6721 taggtgaggt ggttggcttt ggtgcctttg ccgatggcgg cgtttttgat ttcgacgaag
     6781 ttgccgacgt gtacgtcgtt agctagtttg gcttgcggac gcaaacgggc gtatgggccg
     6841 attcggttgt tttcgccgac ttcgcagtct tcgaggtggg agaagggggc gattttgctg
     6901 tttgcaccga ttttggcgtt tttgatgacg cagtttgcgc cgatttcgac gttgtcgccg
     6961 agctcgattt cgccttcaaa gatacagttt gcatcaatca cgacatcctg tccgtgtttc
     7021 agacggcctc gtaaatcgaa acgtgccgga tcgcgcaggg ttacgcctgc tttgagcaat
     7081 tcttgcgcct gttcggtttg gaagatgcgt tcgagttcgg cgagctggcg tttgttgttc
     7141 acgccggcgg cgaggtagga ggcgcgcact tggacgggat ggactttaat accatcggca
     7201 acggctttgg cgatgaggtc ggtcagatag tattcgcctt gtgcgttgtt gctggagagg
     7261 ctgttcagcc agttttccag tttggcgttg ggcaggacga ggatgcctgt gttgatttct
     7321 ttcacggctt tttgggcggc atcggcgtct ttttcttcga cgatggcggt tacgctgccg
     7381 ttgccgtcgc ggatgatgcg ccccaagcct gtcgggtcgg cgggcacgtc ggtcaacagc
     7441 ccgacttcgt tgcctgcggc ttcgagcagg gtttcgaggg tttcaacgtc aattaaagga
     7501 acgtcgccgt acagcaccag cgtgcggcct tcggcggcaa ggtggggcag ggcggttttg
     7561 acggcgtggc cggtgccgag ctgttcggtt tgttcaaccc aaacgacatc gcgtttgaca
     7621 gtattcaaaa cttgctcttt gccgtgtccg atgacgacgc agatgttttg cggattcagt
     7681 gcggcggcgg tgtcgataac gcgctcaacc atgggcttgc cgccgatgca gtgcagcact
     7741 ttgggcattt tggaatacat gcgcgtgcct ttgccggcgg cgaggatgac gatgtttaaa
     7801 gtgttttgcg gcatagcgtt ttcctgtgca atgccgtctg aaagggcttc agacggcata
     7861 gggtgggttt atcggttttg aaatttgggc ttttgccagt gttggcgatg ctcttcgtcg
     7921 gcgttgttgc cggtttggtt gggtaacacg gcatggcgtt cgggacggta ttggttgtag
     7981 ttcatatttt tcgagtagct gccgtcttgg taataaacgg gcgtgccggc gggatatttt
     8041 tgacggacgg cggtcttgcc gttgccgtct tgataagttt cccacgcgca gcccgacaaa
     8101 agggcggcgg cagtcatcag gataaggaag gttttacgca taacattctt tcgtattttc
     8161 gggggttgtg cgagtattgt aatgattttg gcggtgttct gacaaagttt ctgcataccg
     8221 agccagttgc gccatatcgc ttacggaagc atcgataaag ggcaggtcgt gcacttttgc
     8281 accgaaccgg acggttttca tgcccaatgc ttttgcctga tgcaggttgt ccgcgctgtc
     8341 gtccaccata atgcagcatt cgggcggtac gtccaaaagg cggcagacat tgagatacgc
     8401 ttgcggattg ggtttgtaca gcagcccgaa atcatccgtg ccgaacagtg cgtcgaaacg
     8461 gttttccaaa ccgagtgcgc cgacaacggc acggacgtaa aacgacgggc cgttggaaaa
     8521 aaccgccttg cgcccgttca gacggcatag ggtgttttcc gtatccgcca tgccgtgcag
     8581 cctggtcagg attgcatcga tcggatggct ttcgcgcaaa aattcggcga tgtcgatttc
     8641 gggatggtgg atttgcagtc cggcgagcgt tgcgccgtag cggtgccaat agtcttgacg
     8701 caggtcggac gcggcagatt cggagagttt gaggcggcgt gccatatagc gtgtcatggc
     8761 gcggttgatg agtgtgaaga tgccggcatc ggcattgtgc agcgtgttgt cgaggtcgaa
     8821 cagccacacg gtcgggtttt cttgcatttt gaaccgtgaa aatttgttag aatgttattt
     8881 tacagcgaat agaggaggac tcggaatgaa acggaaaatt tggctgctgc cgctgctggc
     8941 ggtttcggca tacctgcagg cgcagacgga agtcaggctg gcggtgcata agtcgttcag
     9001 cctgcccaaa gggctgattg cgcgcttcga gcgggcaaac gatgcgaagg tgtcgattat
     9061 tcaggcgggc ggcgcgaacg aaatgctcaa caaactgatt ttgagccgcg ccaacccgat
     9121 cgccgacgcg gtgtacggct tggacaacgc caatatcggc aaggcgcggg aaatgggcat
     9181 tttggcggcg gcgcaacccg gatccgcccc cgtcgcggtc gggctgcctt cggctttggc
     9241 ggtcgattac ggctatgtgt ccatcaatta cgacaaaaaa tggtttgaag gcaaaaagct
     9301 tcctctgccg caaaccctgc aggatttgac ccgccccgaa tataaaaacc tattggtcgt
     9361 gccgtctccc gccacgtcgt ccccggggct gggcttcctg atggcgaaca tcagcggtct
     9421 gggcgaagaa ggcgcgttca aatggtgggc acagatgcgg cagaacggcg tgaaggtcgc
     9481 caaaggctgg agcgaggcgt attacaccga cttttcgcac aacggcggcg cgtatccgct
     9541 ggtggtcggt tatgccgcca gcccggcggc ggaagtgtat ttttccaaag gcaaatacag
     9601 cgagccgccg acgggcaacc tgtttttaaa aggcggcgta ttccgccagg tcgaaggcgc
     9661 ggcagtcttg aagggcgcga aacagccgaa gctggcggca aaactggtgc aatggctgca
     9721 aagtcgggaa gtgcagcagg cggttccgtc cgaaatgtgg gtttaccccg ccgtcaaaaa
     9781 cacgcgcctg cccgacgtgt tccgcttcgc ccaagccccg acgcacacca ccgcccccgc
     9841 gcagcgcgat attgatgcga accagcgcgg atgggtttcc cgttggatta gaacggtttt
     9901 gaaataaaac aaacataccg ccccgcaggg cttcatacgg catttttaca cctgtgccga
     9961 ttacgccgca cggggcggat gttcgatcaa gaggaaaaca atggacttca aacaatttga
    10021 ttttttacac ctgatcagtg tttccggttg ggagcatctg gctgaaaagg cgtgggcgtt
    10081 cgggctgaac cttgccgccg cgctgcttat ttttttggtc ggaaaatggg cggcgaaacg
    10141 cattgtcgct gtgatgaggg cggcgatgac gcgcgcgcag gtcgatgcca cgctgattag
    10201 ttttttgtgt aatgttgcca atatcggctt attgattttg gtgattattg ccgcattggg
    10261 cagattgggc gtttccacaa catccgtaac cgccttaatc ggcggcgcgg gtttggcggt
    10321 ggcgttgtcc ctgaaagacc agctgtccaa ttttgccgcc ggcgcgctga ttatcctgtt
    10381 ccgcccgttc aaagtcggcg attttatccg cgtcggcggt tttgaaggat atgtccgaga
    10441 gattaaaatg gtgcagactt ctttgcggac gaccgacaac gaagaagtcg tgctgcccaa
    10501 cagcgtggtg atgggcaaca gcatcgtcaa ccgttccaca ctgccgctgt gccgcgccca
    10561 agtgatagtc ggcgtcgatt acaactgcga tttgaaagtg gcgaaagagg cggtgttgaa
    10621 agccgccgtc gaacacccct tgagcgttca aaacgaagag cggcaggccg ccgcctacat
    10681 caccgccttg ggcgacaatg ccatcgaaat cacattatgg gcttgggcaa acgaagcaga
    10741 ccgctggacg ctgcaatgcg acttgaacga acaagtggtc gaaaacctcc gcaaagtcaa
    10801 tatcaacatc ccgttcccgc aacgcgacat acacatcatc aattcttaaa cgccgtctga
    10861 aaggggagtg ggaaatggac gcgctgcaca ccatcgcccg aaacctgacg aaaaaacgtc
    10921 aaaccgtaag ctgtgccgag tcctgtacgg gcggaatgct tgccgccgca ttcacaagcg
    10981 ttgcaggcag ttcgcaatgg ttcgaccaga gttttgtaac atacagcaac aaagccaaag
    11041 aagaccgctt gggcgtgttg cccgaaaccc tgctcgaaca cggcgcggtc agccgctaaa
    11101 ccgtctatga gatggcgcgc ggcgcgaaag ccgtggcgca ggcggattac gccgtcggta
    11161 tttccggcat cgccggcccg ggcggcggca gcgaaagcaa acccgtcggc acggtttggt
    11221 tcgggtttgc ctttccaggc ggaagttgcg aagcaatgcg ccgttttgac ggcaaccgcg
    11281 aatccgtccg tgcgcaggcg gtcgccttcg cattggaacg gttggcgggg ctgattgaaa
    11341 acggcggcga tgccgtctaa acaaaatctc cgtctggaca gaatccccgc cggataaaaa
    11401 ataccgtctg aaacgttgca ggtttcagac ggtattttgt cgggttaggc ggcggtgcgg
    11461 cttatttcac tttactcttc aacgcgccgt agcctgccgc gtccatttgt tccagcggga
    11521 tgaatttcaa gctcgcgccg ttgatgcagt agcgcagtcc gcctttgtcg cgcgggccgt
    11581 cggggaagac gtgccccaag tgcgaatcgg cggcgtggct gcgcacttcg gtgcggcgca
    11641 tgttgtagct gaaatcatcg tgttcggtaa cggattttgc atcaatcggg cgcgtgaagc
    11701 tcggccagcc gcagccggaa tcatatttgt cggcggagct gaacaaaggt tcgccgctga
    11761 caacgtccac ataaatgccg ggtttgaaca aatggtcgta ttcgtggctg aaggcatatt
    11821 cggtcgcgct gttttgggta acttggtatt gctcttcggt cagggtgcgt ttgagttcgg
    11881 cgtcactcgg ttttttatac gttgccgcat cgaagccttt gccttgcggg gcggtcttgg
    11941 ttttgcccgg cagcggttcg tcggctttgc ggatatcgat gtggcagtag ccgttggggt
    12001 ttttaatcag gtagtcctga tggtattcct cggcatcgta gaagtttttc agcggctcgt
    12061 tttcaacaac gaggggcagt tggtattttt gctgctcgcg tttgagggcg gcggcgatga
    12121 cggctttttc ggcggggtcg gtgtagtaca cgccgctgcg gtattgcgta ccggtgtcgt
    12181 tgccctgttt gttgaggctg gtcggatcaa cgacacggaa gaaatattgc aggatgtcgt
    12241 ctagactgag tttgtcggca tcgtaggtca ctttgacggt ttcggcgtgg cccgtatggc
    12301 ggtaggacac gtcttcatag ctcggatttt tcgtgttgcc gttggcgtag ccggataccg
    12361 cgtcaaccac gccgtcgatg cgttggaaat aggcttccaa gccccagaag cagccgccgg
    12421 cgaggtagat ggtgcgtgta ttcatgattg ctgaatcctt tttctgagtg tcgggtttgt
    12481 agaacgaatg tttcaaactg cccaaatcgg cattcgggtc gcggattaac gccaacgcct
    12541 gcgcttcgtt gatgctgcct ttgacgatgc gctgcacgtc gccgtcttta ccgattaacg
    12601 cccatgaggg gtaaacgctg atattcaggc tttgggcaat cgtgccgccg ttgtcggtca
    12661 cgacgggcag cttgggataa ttcaaaccgg cataccattt ttgaaagtcg ccgtcttttt
    12721 tctcgtgcaa aaagcccggg gaggcgacgg taatcaggtt ggcggagctg aattttgcat
    12781 cttgcgccca tttttcggtc tgtcccaatt cggacagaca caaaggacac cagctcgccc
    12841 aaaatttaat cagcgtcggt ttgtcttttt tcaagtaaac actggcgggg cggttgtccg
    12901 cggttttcaa agtggataaa gtgtgcggca cggtcgcggc tccggcatcg acgattttgg
    12961 gcgaacaagc acccagcgca agcaggcagc cgaacttggc gcaaagggaa aagaaagtac
    13021 ggtgtttcat tttgatgttt cctgtgtgga cggtttgcat gattagacgt ttgagatgcc
    13081 gaaaccttac agcccggatt ttcagacaac cctgccgcgt aaaatacgct acaatacgcc
    13141 ctatttcaag tttctaaaat taaaaggaaa atccaatgtt cagcttcttc cgtcgcaaga
    13201 aaaaacagga aacgccggct ccagaggagg ctcaaattca ggaaaccgca gcaaaagcag
    13261 aatccgaaac tgctcaagta attgaaaata ttaaagaaga tgttaattct ttggcggaaa
    13321 gcgtcaaagg gcaggtcgaa tctgccgttg aaaccgtcag cggtgcggtt gaacaggtaa
    13381 aggaaaccgt tgccgagatg ctgtctgaag cagaggaagc ggcggaaaaa gcagcggaac
    13441 aagtcgaagc ggcaaaagaa gccgttgccg aaaccgtcgg cgaggctgtc gggcaagttc
    13501 aagaagccgt tgcgacaact gaagaacaca agctcggttg ggcggcgcgt ttgaaacaag
    13561 gtttgaccaa atcgcgcgac aaaatggcga aatcgctggc gggcgtgttc ggcggcggac
    13621 aaatcgacga agatttgtac gaagagctgg aaaccgtgct gattaccagc gacatgggca
    13681 tggaagccac cgaatacctg atgaaagacg tgcgcgaccg cgtcagcctc aaagggctga
    13741 aagacggcaa cgaattgcgc ggcgcgttga aagaagcctt gtatgacctg attaagcctc
    13801 tggaaaaacc attggtcttg cccgaaacca aagagccgtt tgtcatcatg cttgccggca
    13861 tcaacggcgc gggcaaaacc acgtctatcg gcaaactcgc caaatatttc caagcgcagg
    13921 gcaaatctgt gctgcttgcc gccggcgaca ccttccgcgc cgccgcccgt gagcagcttc
    13981 aagcttgggg cgaacgcaac aacgtaaccg tgatttcaca aaccacgggc gattccgcag
    14041 ccgtgtgctt cgatgccgtc caagccgcca aagcgcgcgg catcgacatc gtgcttgccg
    14101 acaccgccgg ccgcctgccc acgcagcttc acttgatgga agaaatcaaa aaagtgaaac
    14161 gcgtgctgca aaaagccatg cccgacgcgc cgcacgaaat catcgtcgtg cttgatgcca
    14221 acatcgggca aaacgccgtc aaccaagtca aagcctttga cgacgcatta gggctgacgg
    14281 ggcttatcgt taccaaactc gacggcacgg caaaaggcgg catcctcgcc gcgcttgctt
    14341 ccgaccgccc cgtccccgtc cgctatatcg gcgtgggcga aggcatagac gatttgcgcc
    14401 cgtttgacgc gcgcgcgttt gtggacgcac tgctggattg agccgaaatg ccgtccgaaa
    14461 acagcagacc gatgccgtca ttcccgcgca ggcggaaatc cagaccttgg gataacggca
    14521 atattcaaag tttataaaag acccgtcatt cccgcgtagg cgggaatcca gaccttggga
    14581 taacagcaat attcaaagtt tataaaagac ctgtcattcc cgcgcaggcg ggaatctaga
    14641 accatcggca cggaaactta tcgggtaaac ggtttcttga gattccgcgt cctagattcc
    14701 cactttcgtg ggaatgacgg gattttaggt ttctgatttt gtttttctgt ttttgtagga
    14761 atgatgaaat tttgagtttt aggaatttat cggaaaaaac agaaaccgct ccgccgtcat
    14821 tcccgcgcag gcgggaatct aggtttgtcg gtgcggaaac ttatcgggta aaacggtttc
    14881 tttagatttt gcgttctaga ttcccacttt cgcgggaatg acgaagagtt gcgggaatga
    14941 tggaaagcta tgggaataac gaagggttaa agtaatcacg ggatggtgtt cgcgggaata
    15001 taaattgaaa taattcaaaa gggtattata tgcagcctgc ggtttatatt ttagcaagcc
    15061 aacgtaatgg cacgttatac attggcgtta catctgattt ggtgcaacgt atttaccaac
    15121 atagggagca tttgattgag ggatttacat cacggtacaa cgttactatg ctggtttggt
    15181 atgaactgca tcctacgatg gagagtgcaa ttactcggga aaaacagttg aagaaatgga
    15241 acagggcttg gaaattgcaa ctgattgaag aaaataatgt ttcttggcag gatttatggt
    15301 ttgatattat ttagcccgtc attcccgcgc aggcgggaat ccggcttgtt cggtttcggt
    15361 tttttttttg aggtttcggg caacttctaa accgtcattc ccgcgcaggc gggaatctag
    15421 accttagaac aacagtaata ttcaaagatt ataaaagacc cgtcattccc gcgcaggcgg
    15481 gaatctaggt ctgtcggcac ggaaacttat cgggtaaacg gtttcttgag attccgcgtc
    15541 ctggattccc actttcgtgg gaatgacggg attttaggtt tctgtttttg gttttctgtt
    15601 tttgtaggaa tgatgaaatt ttgagtttta ggaatttatt ggaaaaaaca gaaaccgctc
    15661 taccgtcatt cccgcgcagg cgggaatcca gaccttagaa taacagcaat attcaaagat
    15721 tatctgaaag tccgggattc tagattccca ctttcgtggg aatgacggaa tgtaggttcg
    15781 cgggaatgac gcggtgcagg tttccgtgcg gatggattcg tcattcccgc gcaggcggga
    15841 atccagacct tgggataaca gcaatattca aaggttataa aagacccgtc attcccgcgc
    15901 aggcgggaat ctagacctta gaacaacagt aatattcaaa ggttagctga agctttagag
    15961 attctggatt cc
//
LOCUS       DAWFKN010000044        15938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFKN010000044 DAWFKN010000000
VERSION     DAWFKN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.044"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..79)
                     /locus_tag="WGR46_001314"
     CDS             complement(<1..79)
                     /locus_tag="WGR46_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0856254.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGR46_001315"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGR46_001315"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0856255.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGR46_001316"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGR46_001316"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0856256.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGR46_001317"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGR46_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0856257.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQSERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WGR46_001318"
     CDS             4804..5868
                     /locus_tag="WGR46_001318"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0856258.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5888..6850
                     /locus_tag="WGR46_001319"
     CDS             5888..6850
                     /locus_tag="WGR46_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856259.1"
                     /translation="MPSEPPFGRYLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTTFCCLSLTAALILTACSSGGGGVAADIGAGLADVLTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTALQTEQEQDLEHSRKMVAKRRFKIGDIAGEHTSFDKLPKDVMATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAVAYIKPDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGEKAQEVAGSAEVKTANGIHHIGLAAKQ"
     gene            6990..7667
                     /gene="tsaB"
                     /locus_tag="WGR46_001320"
     CDS             6990..7667
                     /gene="tsaB"
                     /locus_tag="WGR46_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0856260.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AVASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRTADIALPEGHVFSDG
                     IGNAFALENRPPFAGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTVKEQ
                     AERKART"
     gene            7664..8104
                     /gene="rimI"
                     /locus_tag="WGR46_001321"
     CDS             7664..8104
                     /gene="rimI"
                     /locus_tag="WGR46_001321"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0856261.1"
                     /translation="MNIRRPSPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8098..8832
                     /locus_tag="WGR46_001322"
     CDS             8098..8832
                     /locus_tag="WGR46_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0856262.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            8894..9535
                     /gene="pyrE"
                     /locus_tag="WGR46_001323"
     CDS             8894..9535
                     /gene="pyrE"
                     /locus_tag="WGR46_001323"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0856263.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9609..10043
                     /locus_tag="WGR46_001324"
     CDS             9609..10043
                     /locus_tag="WGR46_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856264.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10040..11350
                     /gene="argA"
                     /locus_tag="WGR46_001325"
     CDS             10040..11350
                     /gene="argA"
                     /locus_tag="WGR46_001325"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0856265.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11446..13461
                     /locus_tag="WGR46_001326"
     CDS             11446..13461
                     /locus_tag="WGR46_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0856266.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEARELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13575..14531)
                     /locus_tag="WGR46_001327"
     CDS             complement(13575..14531)
                     /locus_tag="WGR46_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856267.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14767..15732
                     /locus_tag="WGR46_001328"
     CDS             14767..15732
                     /locus_tag="WGR46_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856268.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         3549 a         4844 c         4370 g         3175 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacaac cggtttcata cacatctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaacc
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctta tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg caacgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatatc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caataccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt atagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccgta ttggcgcatg gtgtgcatct ggtttttgcg tccggtaagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgttg
     3421 tacacatagt gcagggcgat ggtcagctcg accgcaccta agttgctggc gaaatgaccg
     3481 ccggtttgac cgacggattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagtgct
     4261 ttgggtacgc gctcggactg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggca gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaaccggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat ttacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatacctgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgccggt
     5881 aaacaaaatg ccgtctgaac cgccgttcgg acggtatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagta aacctgtgaa ccgaactacc ttctgttgcc tttctttgac
     6061 cgctgccctg attctgaccg cctgcagcag cggagggggc ggtgtcgccg ccgacatcgg
     6121 tgcggggctt gccgatgtgc taaccgcgcc gctcgaccat aaagacaaag gtttgcagtc
     6181 tttgacgctg gaccagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct taatacgggc aaattgaaga acgacaaggt
     6301 cagccgtttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caagtgtaca aacaaagcca ttccgcctta accgcccttc agaccgagca
     6421 agaacaagat ctagagcatt ccaggaagat ggttgcgaaa cgccggttca aaatcggcga
     6481 catagcgggc gaacatacat cttttgacaa gcttcccaaa gacgtcatgg cgacatatcg
     6541 cgggacggcg ttcggttcag acgatgccgg cggaaaactg acctatacta tagattttgc
     6601 tgccaaacag ggacacggca aaatcgaaca tttgaaatcg ccggaactca atgtcgatct
     6661 ggccgtcgcc tatatcaagc cggatgaaaa acaccatgcc gtcatcagcg gttccgttct
     6721 ttacaaccaa gacgagaaag gcagttactc cctcggtatc tttggcgaaa aagcccagga
     6781 agttgccggc agcgcggaag tgaaaaccgc aaacggcata caccatatcg gccttgccgc
     6841 gaagcaataa ccattgtgga aatgccgtcc gaacacgata atttaccgtt cggacggcat
     6901 ttttgcgcca taccgcccta ggacacgacg gcacggcagg caagccggtt tcactatata
     6961 atgccgtctg aaccaacgag aaccacacca tgcaagccga tttcaaccgc cccgtcctcg
     7021 ccgtcgatac cggaacttcc tatttgtcgc tcgcgctgcg tgccgacggc gaaacccgtc
     7081 tgttccatca ggaagccggc agccgccagt ccgaactgat tctgccggaa atccgcacgc
     7141 tgtttcacga tgcgggcatt accgccgccg atttgggggc gattgtttat gcgaaaggcc
     7201 ccggcgcgtt taccggactg cgtatcggta tcggcgtggc gcaaggtttg gcaacgccgt
     7261 tcaatacgcc tttaatcggc atccccacgc tcgatgccgt cgcctcgctg ccgccgccgc
     7321 aaagctgcat actcgccgct gcggacgcac gtatgggcga agtgttttat gcgtggttcg
     7381 acacgctgaa ccgccgccgt ttgagcgatt atcaagtcgg taggacggca gacattgccc
     7441 tccccgaggg acacgtcttt tcagacggca tcggcaatgc gttcgcgctg gaaaaccgtc
     7501 cgcccttcgc aggcaaaccc gatatgccga ccgccgccga ttttctcaat ctggcggaag
     7561 aaggcggttt tcccgccgtc gctgcggcac acgccgagct gctctacgtc cgcaacaaaa
     7621 tcgccttgac tgtcaaagaa caggcggaac ggaaagcccg aacatgaaca tccgccgccc
     7681 ctcaccgtcc gactgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg
     7741 gacgcaacgc caatttgagt ccgcactggt ttcgccgtcc gaacaggttt tccttgcgga
     7801 aaaagacggc gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact
     7861 gcacctgatt gccaccgcgc ccgaatgccg ccgccaagga attgcgtccg ccctgctcga
     7921 atattggtac gcacacctgc ccgaaggcac gcaacgcctg ctgctcgaag tccgcgcagg
     7981 caacaccgcc gcacaggcac tgtacgcggc gcacggcttc agcattgcgg gcaggcggaa
     8041 aaactattac cgtacagccg acggtaaaac cgaagatgcc gtcttgatgg agaaaatatg
     8101 ttaagcgcgc gctacctcca cctgcacgaa gctttgggtt taggcccgat gtggctgaaa
     8161 caggccgccg ccgtcctgcc gcccaaaaac acacccgcac cctcggcaca ggcacgtcct
     8221 caaaccgtcc gcgccgcccc gatccgccct tcccaacccc ataacggtca ggcacggctc
     8281 gaaacgatga aagcgttgga aaccgccgcc gtacatacgc gcaaacccgc gcctgaaacc
     8341 gaaacgcctc tgcccggcct ttcagacggc atcgcccccg ttcccgccgc ttcgggcatc
     8401 accaagcttg ccgtcgtcag cctttgccca ccgatcgagg atgcggttta cgggcaactg
     8461 ttccacggca aagcaggtgt tctgcttgac aatatactca aagccgtagg actggatgcc
     8521 gcttatgtac acaagaccag ctgggtgaaa accgccgccg tcggcaaccc gatgccgtct
     8581 gaacaggccg tcgcgaatgc gctgggtcaa atcgccaggg aactcgacgg ctgccgtgcc
     8641 cccgccgtcc ttttcctcgg gcaggctttt gtccagcctg aacggcaaac gatgattgaa
     8701 actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgctcggct tttacgccaa
     8761 cccgaactta aagcccgaaa ctggcaggtg ttgaaacagt tgaaacacgc cttgcaggca
     8821 ggcggcagtt gaagcaggcc gcacggggcg gtagaatcgc aactgcgtcc caatatctga
     8881 cagaaagcac aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa
     8941 tgttttgaaa ttcggcgaat tcaccaccaa agccggacgg cggtcgccct atttcttcaa
     9001 tgccggcctc tttaacgacg gcttgtccac gctgcaactg gcaaaattct atgcacaatc
     9061 catcattgaa agcggcatcc gattcgatat gctgttcggt cccgcctaca aaggtattat
     9121 tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta
     9181 caaccgcaaa gaagccaaag accacggcga aggcggcgtg ttggtcggcg cgccgcttaa
     9241 agggcgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat
     9301 caaactgatt gaagcggagg gtgcaacccc cgccggtgtc gccatcgcgc tcgaccgcat
     9361 ggaaaaaggc acgggtgaat tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc
     9421 cgtcgccccc atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt
     9481 cggacagttc ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca
     9541 aagcatatgc cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag
     9601 gaaataccat gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa
     9661 cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag
     9721 ccaaagacca tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt
     9781 cggatgtcaa actcgttcac cgcatcggca cgcgcgccat tggcaagaaa cagatttccc
     9841 gcgacgaaat cgccggcatc ctcaacggcg gtacaaccct gcacgatacg ccgcccgcaa
     9901 ccgccgccac ccctgccgcc gcaccgcagg ttgccgtacc gcccgccgcg cctgcccgtc
     9961 aggaagggtt caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc
    10021 tttcctacct cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc
    10081 cgtcccctac atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct
    10141 gctcgaaggt gataccttaa acaagctcgc cgccgacatc gggctgttgt cgcaactggg
    10201 catcaggctc gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca
    10261 aggccgcacg ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca
    10321 ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt
    10381 ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg
    10441 tccgataggt gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga
    10501 caccgccgcc ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg
    10561 acattcctac agcggcaaga ccttctatct cgatatgctt caaaccgccg cctccgtcgc
    10621 cgtctcgctt caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc
    10681 cgacggcacg ctcgccgaaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc
    10741 cggcggcgaa acgcgacggc tgatttcgtc caccgttgcc gcgctcgaag gcggcgtgca
    10801 tcgcgtccaa atcctcaacg gtgcctccga cggcagcctg ctgcaagaac tcttcacccg
    10861 caacggcatc ggcacatcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag
    10921 cggcgacatc ccgcacatcg ccgccctcat ccgcccgctg gaagaacagg gcatcctgct
    10981 gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga
    11041 cggcaacctg tacggttgcg ccgccctgaa aacctttgcc gaagccgatt gcggcgaaat
    11101 ctcctgcctt gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc
    11161 ccacattatc gataaggcgc gcagtatggg cataagcagg ctgttcgccc tctccacaaa
    11221 taccggcgaa tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga
    11281 aacgcggcgc aaagactacc gcagcaacgg acgaaactcg catattctgg tacgtcgcct
    11341 gcaccgctga ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg
    11401 gcatttcccc gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc
    11461 gacccctacc gccattttga aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa
    11521 gcgaatgccg aaacgcgcgc gcgtttttta aacaacgaca aggcgcgcgc gctttcagac
    11581 ggcattttga atcaaatgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg
    11641 cggatgtacc atttccatca ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgcacg
    11701 gcggcgacct accgttcggg ctatccggag tggaaaatcc tgttttcagt ggcggacttc
    11761 gacgaattgc tcggcgacga tgtatatttg ggcggcgtgt cgcacttggt ggaaaagccc
    11821 aaccgcgcgt tgttgacact aagcaaatca ggcggcgata cggcgtacac gctggaagtg
    11881 gatttggaag caagggaatt ggtagaaggt ggttttcact ttccggcagg caaaaaccaa
    11941 gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg cttgggacga acgccagttg
    12001 accgaatcgg gctatccgcg tgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa
    12061 agcctgcctg tgtatcaaat cgataaagat gcaatgatgg tgaacgcgtg gcgttatctc
    12121 gatccgcagg gttcgccgat tgatttgatt gaagcgtcgg acggttttta caccaaaacc
    12181 tatttgcagg tgtcggccga aggcgaggcg aaaccgttaa acctgcccaa cgattgcgat
    12241 gtggtcggct atctggcggg gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg
    12301 aaccaaagct atccgagcgg tgcgctggtg gcggtgaagc tgaatcgggg tgaactcggg
    12361 gcggcgcagc ttttgtttgc gcctgatgaa acgcaggcat tggaaagcgt ggaaacgacc
    12421 aagcgttttg tggtggcgag cctgctggaa aacgtacaag gccgtctgaa agcgtggcgt
    12481 tttgccgacg gcaaatggca ggaagtcgaa ctgccgcgcc tgccttcggg cgcgttggaa
    12541 atgaccgacc agccttgggg cggcgacgtg gtttaccttg ccgccggcga tttcaccaca
    12601 ccgctgacgc tgtttgcgct ggatttgaac gtgatggaac tgaccgtcat gcgccgccag
    12661 ccgcagcagt tcgattcaga cggcatcgaa gtgcggcagt tttgggtgat gtcgtccgac
    12721 ggcgaacgca tcccttattt ccacgtcggc aaaaacgccg cgcccgacac gccgacgctg
    12781 gtttatgcct acggcggttt cggcgtaccc gagctgccgc attatctggg cagcatcggc
    12841 aaatactggc tggaagaggg caacgccttc gtgttggcga acatacgcgg cggcggcgag
    12901 ttcggcccgc gctggcatca ggcggcgcag ggaatcagca aacacaaaag cgtcgatgat
    12961 ttgcttgccg tcgtgcgcga tttgtccgag cgcggcatca gttcgcccca acacatcggt
    13021 ttgcagggcg gcagcaacgg cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa
    13081 agcataggcg cactggtgtg cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc
    13141 tccgccggtt caagctggac agacgaatac ggcaatccgc aaaaatacga agtctgcaaa
    13201 cgccggttgg gcgaattgtc gccgtatcac aatctttcag acggcatcga ttatccgccc
    13261 gcgctcatca ccaccagcct cagcgacgac cgcgtccatc ccgcccacgc gctcaagttt
    13321 tacgccaaac tgcgcgaaac ctcgccgcaa tcttggctct actcgcctga cggcggcggc
    13381 cataccggca acggcacgca gcgcgaagcc gccgacgaac tcgcctgcgt gttgctgttt
    13441 ttgaaagagt ttttgggcta agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt
    13501 cgtctgaaac tgctttttca gacgaccttt tttaatggtt gtttcaaatc aaaatatcta
    13561 tgccgccggc cccatcagca cttcttcaca tccgaaggca aaaatccgta atgccgtctg
    13621 aacgcttcgt tgaaccgtcc cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg
    13681 gtgctgccgg attcgatgag ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg
    13741 gcgacggttt cgccggtttg cgctttgaaa tagcgtttca ggtagcattc gttcagtccg
    13801 acgcggcggg cgatttcggc gatggtcagc ggacgggcga attcgtgttg caggatgtcg
    13861 gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc
    13921 aataatcggg cggagagttc cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc
    13981 gattcgaacg ggcgttgcag cagtgggcag gccgccgccg tcagtgccgc cgcgttttgc
    14041 gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc
    14101 aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac
    14161 atgccgctgt tgtcgggcag cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc
    14221 cagatttcgc cggcagacgg gcggtattcc ctgccgccca tttgtaaccg gttctgcccc
    14281 gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg
    14341 cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac
    14401 atttccagat gcaaaccggg ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg
    14461 tccaacgccc agccggattt atcggagcgg acataggttt ggtactggcg gtagatggcg
    14521 gcggtgttca tgattggata ggaacgagtt gtctaacaaa tgaattaaat aggaattatt
    14581 accaataatc aagcgcaggg attggttgaa acggaaaagg tcgtctgaaa gggtgtttca
    14641 gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg ggaattttgc gttttgcggc
    14701 gtggtttctg caggttgttt gcttaataat aaacattctt attcgtatgc aaaggaaccg
    14761 cacaccgtga aaccgcgttt ttattgggca gcctgcgccg tcctgctgac cgcctgttcg
    14821 cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg catctgccgc cacactgacc
    14881 gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac
    14941 gactgggcgg cgttggatac gctgaccgaa ttgggcgtga atgtgggcgc aaccaccgcg
    15001 ccggtgcgcg tggattattt gcagcctgca tttgacaagg cggcaacggt ggggacgctg
    15061 ttcgagcccg attacgaagc cctgcaccgc tacaatcctc agcttgtcat taccggcggg
    15121 ccgggcgcgg aagcatatga acagttggcg aaaaacgcga ccaccataga tctgacggtg
    15181 gacaacggca atatccgcac cagcggcgaa aagcagatgg agaccttggc gcggattttc
    15241 ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg
    15301 cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt cggttacggg caacaaggtg
    15361 tccgccttcg gcacgcagtc gcggttggca agttggatac acggcgacat cggcctaccg
    15421 cctgtagacg aatctttacg caacgagggg cacgggcagc ctgtttcctt cgaatacatc
    15481 aaagagaaaa accccgattg gattttcatc atcgaccgta ccgccgccat cgggcaggaa
    15541 gggccggcgg ctgtcgaagt attggataac gcgctggtac gcggcacgaa cgcttggaag
    15601 cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg tcgcgggcgg ctcgcggcag
    15661 ttgattcagg cggcggagca gttgaaggag gcgtttgaaa aggcagaacc cgttgcggcg
    15721 gggaaagagt agggagtcgt ctgaaaacgg agtttccgaa ggaagcgggg ggtttctgcg
    15781 aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa
    15841 gcaaacctac ggtttgccgc cctctcccta gccctctccc acagggagag gggattgggg
    15901 tgcaggctgc ctttaaggtt taggcaaatt tttaactt
//
LOCUS       DAWFKN010000045        15875 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFKN010000045 DAWFKN010000000
VERSION     DAWFKN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.045"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            101..220
                     /locus_tag="WGR46_001329"
     CDS             101..220
                     /locus_tag="WGR46_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0856269.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            233..1096
                     /locus_tag="WGR46_001330"
     CDS             233..1096
                     /locus_tag="WGR46_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0856270.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGR46_001331"
     CDS             complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGR46_001331"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0856271.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2303..2392)
                     /locus_tag="WGR46_001332"
     tRNA            complement(2303..2392)
                     /locus_tag="WGR46_001332"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2356..2358),aa:Leu,seq:taa)
     gene            complement(2495..2568)
                     /locus_tag="WGR46_001333"
     tRNA            complement(2495..2568)
                     /locus_tag="WGR46_001333"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Cys,seq:gca)
     gene            complement(2594..2669)
                     /locus_tag="WGR46_001334"
     tRNA            complement(2594..2669)
                     /locus_tag="WGR46_001334"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Gly,seq:gcc)
     gene            complement(2691..2766)
                     /locus_tag="WGR46_001335"
     tRNA            complement(2691..2766)
                     /locus_tag="WGR46_001335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2731..2733),aa:Gly,seq:gcc)
     gene            complement(2790..2865)
                     /locus_tag="WGR46_001336"
     tRNA            complement(2790..2865)
                     /locus_tag="WGR46_001336"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2830..2832),aa:Gly,seq:gcc)
     gene            complement(2891..2966)
                     /locus_tag="WGR46_001337"
     tRNA            complement(2891..2966)
                     /locus_tag="WGR46_001337"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2931..2933),aa:Gly,seq:gcc)
     gene            complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGR46_001338"
     CDS             complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGR46_001338"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0856272.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3715..4138)
                     /locus_tag="WGR46_001339"
                     /pseudo
     CDS             complement(3715..4138)
                     /locus_tag="WGR46_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4178..4288)
                     /locus_tag="WGR46_001340"
                     /pseudo
     CDS             complement(4178..4288)
                     /locus_tag="WGR46_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4420..4755)
                     /locus_tag="WGR46_001341"
     CDS             complement(4420..4755)
                     /locus_tag="WGR46_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0856273.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            4884..5789
                     /gene="mtrA"
                     /locus_tag="WGR46_001342"
     CDS             4884..5789
                     /gene="mtrA"
                     /locus_tag="WGR46_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0856274.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(5851..6339)
                     /locus_tag="WGR46_001343"
     CDS             complement(5851..6339)
                     /locus_tag="WGR46_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0856275.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6486..7388
                     /locus_tag="WGR46_001344"
     CDS             6486..7388
                     /locus_tag="WGR46_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0856276.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7429..8559)
                     /locus_tag="WGR46_001345"
     CDS             complement(7429..8559)
                     /locus_tag="WGR46_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856277.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKVG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPSHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            8763..11387
                     /gene="alaS"
                     /locus_tag="WGR46_001346"
     CDS             8763..11387
                     /gene="alaS"
                     /locus_tag="WGR46_001346"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0856278.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPAERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIDLDEAGFEREMEAQRARARAAQSFKANAQLPYEG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSEQA
                     IVLLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            11449..12180
                     /locus_tag="WGR46_001347"
                     /pseudo
     CDS             11449..12180
                     /locus_tag="WGR46_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12889..15300
                     /locus_tag="WGR46_001348"
     CDS             12889..15300
                     /locus_tag="WGR46_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0856279.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAHRTFQWTSQAAGKAAVH
                     CIIVGFRQKPPMPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPIHCE
                     PDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLWFHG
                     VSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGNYLI
                     IPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAGRLK
                     SDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQEAG
                     LPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFELYRK
                     AAAIA"
BASE COUNT         4030 a         4307 c         3759 g         3779 t
ORIGIN      
        1 ggaggattag gggggtattg gggtaaaatt agtggatatt tgaaacgaaa acagccgaaa
       61 acctgtgttt gggtgtcagc tgtcgggagg gaaaggaatt ttgcaaaggt ctcacaaagt
      121 atttgccaca ttcggcgaca agcgcacaac ccggctgttt gccgatttgg atgcactggc
      181 tgaagtgatg gaaaaaacaa tctcggaaaa taaaaaatag gggggcaaat atgtggaaaa
      241 tgttgaaaca catagccaaa accaccgcaa gcgattgatt ggcacatttt ttctggtcgg
      301 actggaaaac cttttgatgc tggtgtatcc ggtgtttggc ggctgggcga ttaatgccgt
      361 gattgcgggg caggcgtggc aggcgttgct gtacgctttg gttgtgcttt tgatgtggct
      421 ggtcggtgcg gcgcggcgga ttgccgatac gcgcacgttt acgcggattt ataccgaaat
      481 cgccgtgccg gttgtgttgg aacagcggca gcggcaagtc ccgcattcgg cggtaactgc
      541 gcgggttgcc ctgtcgcgtg agtttgtcag cttttttgaa gaacacctgc cgattgccgc
      601 gacatccgtc gtatccatat tcggcgcgtg catcatgctg ctggtgctgg aattttgggt
      661 cggcgtgtcg gcggtgggca tacttgcgtt gtttttatgg cttttgccac gttttgccgc
      721 catcagcgaa aacctgtatt tccgcctgaa gaacagcttg gaacgcgaca accactttat
      781 ccgaaaaggc gacgagcggc agctggaccg ccattacgga ctgcttgcgc gcctgcgtgt
      841 gctgatttcc aaccgcgaag ccttcggcta tctctgcgtc ggcacggcga tgggtatttt
      901 gttcggcttt gcttttgtga tgatgacgct caaaggctac agcagcgcgg ggcatgtcta
      961 ttcggtcggc acttatctgt ggatgtttgc catgagtttg gacgacgtgc cgcgattggt
     1021 cgaacaatat tccaatttga aagacatcgg acaacggata gagtggtcga aacggaacat
     1081 caaagccgga acttgaaaaa tgccgtctga acacgcttca gacggcattt ccatccgttc
     1141 ggcaaactac atcacatccg cccgccggtt gacaagtttg gcaaacaact tttcaacaga
     1201 agcttccgcc tgcaaaccaa tgcgctggat caggctttgc ttctcctgat atttcacttc
     1261 gataacctgt ttgttttcaa acgctttcaa caacaaatca tcactggtcg aaatctcgtc
     1321 aatcaagttc aacgccaacg cctgccgacc gaaccaatgc tcgcccgttg ccacttcctc
     1381 aatatccaat tgagggcggt tctcgctgac aaactgcttg aacaactgat gcgtttcctc
     1441 cagttcctgt cggaatttct gtttgccctt ttccgtattt tcacccataa aagtaaccgt
     1501 gcgcttaaat tcgcccgccg tcatcacatc cacatcaata tcatgttttt tcaacaggcg
     1561 gtggatattc ggtacttccg ctacaacacc caccgaaccg acaatcgcaa acggagcgga
     1621 aacaatttta tccgccacac acgccatcat ataaccaccg ctcgccgcca ccttatcgac
     1681 ggcgacggtc agcggaatat tgcgttcgcg caaacgccta agctgcgaag ccgccaaacc
     1741 gtaaccgtga accacgccgc ccggactttc caatctaagc agaacctcat cttcaggctt
     1801 ggcaatcaaa agcaccgccg taatctcatg acgcaaggat tctacggcgt gtgcatacaa
     1861 atcgccgtca aaatccaaca caaaaaggcg ggatttttgc gtttcggaag atttctcccc
     1921 accctccttc aaacgctttt tctctgcttt ggcttccgcc ttttcctttt tcttttcctc
     1981 tttttcctga tgttttgcct cttccccgct taaaaagaat gcttcaaacg attgccgctg
     2041 ttttttataa ttttccgaaa aatccgtcag tacgacactg ccgctttccg actgtttctt
     2101 actctgtacg atagccaaca caatcagcgc aattgcgccg aacacggtaa gcagttcgag
     2161 caggaaaata ccgtaattca gtaaaatttc tttccacatt gattggattt cctcttgttc
     2221 aggcatgaac atgtcaatat tgtccatcac cgtccgacag ataaaaaaat aaccgcttgg
     2281 agcggcattg tcattttcag cttggtgccc ggagccggaa tcgaaccggc acgggatgtt
     2341 tagtcccgac ggattttaag tccgttgtgt ctacctattt caccacccgg gcatttgtga
     2401 aaggtggagg cgggggcgcg gattttaacc ggcctatatg aagattgcac tcctcatagc
     2461 ataaacactc tgccaccccg ccgtagtacg ataatggagg cgagagtcgg aatcgaaccg
     2521 gcgtagacgg atttgcaatc cgctgcataa ccactttgct atctcgccct aaaactggct
     2581 tatctaaaaa acttggagcg ggaaacgagt ctcgaactcg cgacctcaac cttggcaagg
     2641 ttgcgctcta ccaactgagc tattcccgcg cgttcaaaca tatcggtttt tggagcggaa
     2701 aacgagtctc gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctat
     2761 tcccgcgttg atatgtttga aataaaactt ggagcgggaa acgagtctcg aactcgcgac
     2821 ctcaaccttg gcaaggttgc gctctaccaa ctgagctatt cccgcaatga ttgtgggaaa
     2881 atgaaatttt tggagcggga aacgagtctc gaactcgcga cctcaacctt ggcaaggttg
     2941 cgctctacca actgagctat tcccgcccga tttcattctc caacatcgaa gagacgcaat
     3001 tattatggat tctgtttttg ccgtcaagct atttttatgt ttttttcagg cgatttcttt
     3061 ccacgccatt ttcagataat acagcatcga ccagactgtc agcaaagatg cgataaacat
     3121 caatacattg ccgataacaa caagattaaa tccgtaaaaa tcgggaatat tcagcagcag
     3181 caggaaaatt gccagcattt gcgcggcggt tttgaactta ccgacggtgg cgacggcaac
     3241 gctgttcctt ttgcccattt gcgccatcca ttcgcgcaat gcggaaatgg taatttccct
     3301 gccgataatg atcatggcaa acaaaacata ggtccggtcg agtttgacca gtaaaagtag
     3361 cgatacggca accatcagct tgtcggcgac gggatcgagg aaggcgccga aatcggaagt
     3421 ctgtttccac agtcttgcca aaaatccgtc aaaccagtcg gttaaggcgg caacggcaaa
     3481 aatgacggcg gcggtgagat taatcgtttc ctccgcgaac cacggaaaag gcaggtaaaa
     3541 aagggctgtc atgacaggaa tgagcaagac cctcaaccat gtgaggaaga tagggagatt
     3601 ccaaggcatc ggttttctct gtgcagactg taaagttgtg attataacgg ttatcctcat
     3661 aacccaaaac gtaaaattgc tgcatgggca ttcccccgcc ccgccaatct gttttcacat
     3721 tcttttcaaa cgcaggaaaa tggcgggcaa taaaagcaaa atacccagtt tcaggcagaa
     3781 aacggcaggt tgtgccaaca cttcgacaag gcggtcttcc gtgcgggcaa aatctttatt
     3841 gcttatagac actgccactg ttgcggtatt ccaacagaac gccgtttaaa aaacctttgc
     3901 cgacggtttc gcttaaaacg gctctaacct gctccgccct gatggttctg ccgatattgc
     3961 cgcctgtgca caaactgtcg aacccatagc aggaaagccg gtaatgctgc ccgtctgcat
     4021 ccagtttgat tgcccgtccg ctgcggttgg gggcggtaac ggtcaattcc gcatattcga
     4081 atgttttttc ttgttcgtga aatgccgtca ggtaaggtgc aataaaaacg gcggacaaca
     4141 gcagacagct tatggcggca aaccataccc agcgataata tagtggatta aatttaaacc
     4201 agtacagcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     4261 cctgatttaa atttaatcca ctatatttca cgcttacccc ttgtttctca aatgccgtct
     4321 gaaataagcg gcttaatata ttgtttacag tattgggaag cataacagac aaaatgccgt
     4381 ctgaaatatt ttcagacggc atttcttatc cgaaacggat tatttttgcg tttcaaccgc
     4441 ttccaatgca cgcagggcgt aagtgtaagc ggcaccggca ttcagggcga tggcggttgc
     4501 caatgcgcct gcgatttcgc tgtcggtcgc accggctttg gcggcagcgg cggcgtgaac
     4561 gctgatgcag ctctcgcaac gagtggtaat ggcaacggcg atggcaatca gttcgcgcgt
     4621 tttggcatca agtgcttcgg cggcggcggc ctgttccaat gcgccgtagg cctgcagcat
     4681 tttaggatgc gccttaccca gctcgccgaa cgatttttta accaatgcgg tatgttcttt
     4741 ccaatcttta aacattttct tttcctttct cttgcgttta accctgatac gcgcttgcgt
     4801 atctgttttc gatgtgcgta ttattgcaat tattcagttg tgtttctcgt ttaatcatct
     4861 cattttatgg ttcaaaaaga tttatggaca ttctggacaa actggtcgat ttcgcccaat
     4921 tgacgggcag tgtggatgtg cagtgccttt tgggcggaca atggtcggta cggcatgaaa
     4981 ccttgcaacg cgaaggattg gtacacattg ttacatcggg cagcggctat ctctgcatcg
     5041 acggcgaaac ttccccgcgt ccggtcagta caggggatat tgtatttttc ccgcgcggct
     5101 tgggtcatgt gttgagccac gacggaaaat gcggagaaag tttacaaccg gatatgcggc
     5161 agcacggtgc gtttacggtc aagcagtgcg gcaacggaca ggatatgagc ctgttttgcg
     5221 cccgtttccg ctacgacacc cacgccgatt tgatgaacgg gctgcctgaa accgtttttc
     5281 tgaacattgc ccatccgagt ttacagtatg tggtttcaat gctgcaactg gaaagcaaaa
     5341 aacctttgac ggggacggtt tccatggtca acgcattgtc gtccgtcctg ctggtgctta
     5401 tcctgcgcgc ctatctcgaa caggataagg atgtcgaact ctcgggcgta ttgaaaggtt
     5461 ggcaggacaa acgtttggga catttaatcc aaaaggtgat agacaaaccg gaagacgaat
     5521 ggaatgtcga caaaatggtg gcggctgcca atatgtcgcg cgcgcaactg atgcgccgtt
     5581 tcaaaagccg ggtcggactc agcccgcacg cctttgtgaa ccatatccgc ctgcaaaaag
     5641 gcgcgttgct gctgaaaaaa aacccggatt cggttttgtc ggtcgcactg tcggtaggct
     5701 ttcagtcgga aacgcacttc ggcaaggcgt tcaaacggca atatcacgtt tcgccgggtc
     5761 aataccggaa agaaggcggg caaaaataaa tcggggcttc aaacgcaaat gccgtctgaa
     5821 aaggctttca tacagcattt gcgtaccgcg tcatttcaag ggctgcatct tcatcacttc
     5881 catcaaaaag ttggtaaatg cggggttgtt gggtttgaca tccatatttt tccaacgctg
     5941 ctgccagccg cgcaaggcat tctggatata cagcttggac tgttccgtat tgattgcgcc
     6001 ccgctggctg tctatcgccg aacgcaggta gatttcatac atactgtcat cgacggcatt
     6061 gcgtccgacc aggcgttttc tgaagttgtt cagatattgc gccgcctgaa ccttggtcat
     6121 tttaccgata cccacctgat agcccaagcg cgtcgcttca tcgctgattt tggcaacatc
     6181 cgtccaatgc gaagaggcaa ggcggaaacc ttttgcaggt gcttccgttt tgacggtatt
     6241 gataggattc acggggattt ccgtcaatgt gggcacataa atagactggc agccggaaag
     6301 aactgccaca atggaaagag ggataaggta ttttttcatg cccccattat aatcaagttt
     6361 gccttgagaa aacaaattgt tcggcaagaa aaataaaatt tcggcatcag aagcaggcaa
     6421 aaacacattc cacaagcctt gccgcaaggt ttacaatccg accgtcctta tcgcaacgac
     6481 cgtttatgga taccgcaaaa aaagacattt taggatcggg ctggatgctg gtggcggcgg
     6541 cctgctttac cattatgaac gtattgatta aagaggcatc ggcaaaattt gccctcggca
     6601 gcggcgaatt ggtcttttgg cgcatgctgt tttcaaccgt tgcgctcggg gctgccgccg
     6661 tattgcgtcg ggacaccttc cgcacgcccc attggaaaaa ccacttaaac cgcagtatgg
     6721 tcgggacggg ggcgatgctg ctgctgtttt acgcggtaac gcatctgcct ttggccaccg
     6781 gcgttaccct gagttacacc tcgtcgattt ttttggcggt attttccttc ctgattttga
     6841 aagaacggat ttccgtttac acgcaggcgg tgctgctcct tggttttgcc ggcgtggtat
     6901 tgctgcttaa tccctcgttc cgcagcggtc aggaaacggc ggcactcgcc gggctggcgg
     6961 gcggcgcgat gtccggctgg gcgtatttga aagtgcgcga actgtctttg gcgggcgaac
     7021 cctgctggcg cgtcgtgttt tacctttccg tgacaggtgt ggcgatgtcg tcggtttggg
     7081 cgacgctgac cggctggcac accctgtcct ttccatcggc agtttatctg tcatgcatcg
     7141 gcgtgtccgc gctgattgcc caactgtcga tgacgcgcgc ctacaaagtc ggcgacaaat
     7201 tcacggttgc ctcgctttcc tatatgaccg tcgttttttc cgccctgtct gccgcatttt
     7261 ttctgggcga agagcttttc tggcaggaaa tactcggtat gtgcatcatc atcctcagcg
     7321 gcattttgag cagcatccgc cccactgcct tcaaacagcg gctgcaatcc ctgttccgcc
     7381 aaagataaaa aatgccgtcc gaacatcctt cagacggcat atcgggcttt atttccccgc
     7441 cttcacatcc tgccactggc gcaccataaa cttcaatgcc gccggctgga taggcaccat
     7501 gataaagctg tttttcaaat cctcctcggt tgggaaaatc gtattgtcgt ttttaaattc
     7561 gtcttccatc agctcacgcg ccggcttgct cgaaggcgca taagtaacga aattgccgtt
     7621 tttcgccgac acttccgggt cgaggaagtc gttgatgtat ttgtgcgcgt tggcgacgtt
     7681 tttcgcatct ttcggaatca cgaaagaatc cacccaaatc cccacgccct ctttgggcat
     7741 catcacgcgg attttttcct tgccgcccgc ttcttcggca cggcgtttgg cgatgttcaa
     7801 atcgccgccg aaaccgattg ttacgcaggt atcgccgcgc gccaaatcat cgataaagcc
     7861 ggacgaagta aagcgtttga tattggggcg gtttttcttg agcagggcgg ttgcctccct
     7921 gatgtcttcc gtattgctgc tgttcgggtt tttacccaaa tagttcaaca ccataggata
     7981 gatttccgcc gcgctgtcta aataactgat accgcattgt ttgagtttgg acgtgtattc
     8041 ggggtcgaac accaaatccc actggttgtc cggcagcttg tccgtaccca aagccttttt
     8101 cacgcgttcg gtattgatgg cgaaggtatt tgtcccccaa taaaacggca cggcgtattc
     8161 gtggctggga tcgaccccgt ccatcagcct catcatttcg ggattgaggt gtttataatt
     8221 gggaatcagc gatttatcga ttttctgata cgcacctacc ttaatctgcc tgcccacaaa
     8281 cgcattggac ggcgcgacaa tgtcgtaacc ggattttccg gtcagcacct tgctttccag
     8341 cgtttcatcg ctgtcgtaca catcataagt aaccttgatg ccgtttttct tttcaaaatc
     8401 ggcaacggtt tccggatcga cgtattccga ccagttgtaa attttcaata cgtttcggtt
     8461 ttccgccggc gccggttttt cggcaggcgg tttgtccgaa ccgccgcacg ctgcaagcag
     8521 caaagcagtc aggacggcga gtggcagatg tttggccatt atcattcctt gcatatcggg
     8581 ttggagaaag cggtcattat agccgatatt ggcaacaggg cttcagacgg cattcaaaat
     8641 cccgcaatgc cgtctgaaag ccgccgcttc catagctaga aacagggatt tgcggtaaga
     8701 taccgccgtt cgttttccct gcttttacca tgacaagaca tttgagagac attgaaaaaa
     8761 ttatgaaaac ctccgaactg cgccaaaaat tcctaaaatt ttttgaaacc aaaggccaca
     8821 ccgtcgtccg ctcttccagc ctcgtgccgc acgacgaccc gaccctgctg tttaccaacg
     8881 cgggcatgaa ccagtttaaa gacgtattct taggtttcga caaacgcccg tacagccgcg
     8941 ccaccaccgc gcaaaaatgc gtacgcgcag gcggcaagca caacgacttg gaaaacgtcg
     9001 gctacaccgc ccgccaccac accttctttg aaatgatggg caacttctcc ttcggcgact
     9061 atttcaagcg cgacgcgatc cacttcgctt gggaattcct gacttcccca gaatggttga
     9121 acatccctaa agacaaactc ttggcgaccg tttacgcaga agacgacgaa gcctacaaca
     9181 tctggttgaa cgaaatcggt atgcctgccg agcgcatcgt ccgcatcggc gacaacaaag
     9241 gcgcgaaata cgcatccgac aacttctggc aaatgggcga caccggcccc tgcggcccct
     9301 gctccgaaat tttctacgac cacggcgaag aaatctgggg cggcattccg ggcagccccg
     9361 aagaagacgg cgaccgctgg atcgaaattt ggaactgcgt atttatgcag ttcaaccgcg
     9421 acgaacaagg caatatgaat ccgctgccca agccgtccgt cgataccggc atgggcttgg
     9481 aacgcatggc cgccgtcatg cagcatgttc acagcaacta cgaaatcgac ctgttccaag
     9541 acctgctcaa agccgttgcc cgcgaaaccg gcgcgccgtt cagtatggaa gaacccagcc
     9601 tgaaagtcat cgccgaccac atccgctcct gctcgttcct gattgcagac ggcgtcttgc
     9661 cttccaacga aggccgcggc tacgtattgc gccgcattat ccgccgcgcc gtgcgccacg
     9721 gttacaaact gggtcaaagc aaaccgttct tccacaaact cgttgccgat ttggtcaaag
     9781 agatgggcgg tgcctaccct gaattgaaag aaaaacaagc tcaaatcgaa gaagccttga
     9841 aaaacgaaga aagccgtttt gcccaaactt tggaaaccgg tatggctttg ttggaaaacg
     9901 cgttggccaa aggcggcaaa acactcggcg gcgaaatcat cttcaaactc tacgatacct
     9961 acggtttccc atacgacctg actgccgaca tctgccgcga acgcaatatc gatttggacg
    10021 aagcaggctt cgagcgcgaa atggaagccc aacgcgcacg cgcacgcgcc gcacaaagct
    10081 tcaaagccaa cgcccaactg ccttacgaag gtcaagacac cgagtttaaa ggttatagcg
    10141 aacgccaaac cgaatccaaa gtcctcgccc tctacaaaga cggcgagcaa gtcaacgaat
    10201 tgaacgaagg cgacagcggc gcagtcgtta tcgactttac cccgttctat gcagaatccg
    10261 gcggccaagt cggcgacgtc ggctatatct ttgcaggcga aaaccgcttt gaagtacgcg
    10321 atacccaaaa aatcaaagcg gccgtattcg gccaattcgg cgtacaaact tcaggccgtc
    10381 tgaaagtcgg cgacagcgtt accgccaaag tggatgacga aatccgcaat gccaatatgc
    10441 gcaaccacag cgcaacccac ttgatgcaca aagccctgcg cgacgtattg ggcggacacg
    10501 ttgaacaaaa aggctctttg gttaccgccg aatccacccg tttcgacatt tcccatcccc
    10561 aagcggtcac tgccgaagaa atcgccgaag tcgaacgccg cgtcaacgaa gccattttgg
    10621 cgaacgttgc cgtcaacgcc gccattatga gtatggaaga cgcacaaaaa accggcgcca
    10681 tgatgctctt cggcgaaaaa tacggcgacg aagtgcgcgt actgcaaatg ggcggtttct
    10741 ctaccgaact ttgtggcggc acacacgttt cacgcacggg cgacatcggc ctctttaaaa
    10801 tcatcagcga aggcggtatt gccgcaggcg tgcgccgtat cgaagccatc accggcctga
    10861 acgcactcaa atgggcgcaa gagcaagaac gcttggttaa agacattatt gccgaaacca
    10921 aagcccaaac cgaaaaagac gtactggcga aaatccaagc aggcgcggca cacgccaaag
    10981 cattggaaaa agaattggca cgcgccaaag ccgaactcgc cgtccacgca ggcgcaaaac
    11041 tcttggacga cgcaaaagac ttgggcgcgg ccaaactcgt tgctgcccaa atcgaagccg
    11101 acgcagccgc cctgcgcgaa atcgtaaccg acctgaccgg caaatcagaa caagccatcg
    11161 tattgctcgc cgcagtcaac gacggcaaag tctccctgtg cgcaggcgta tccaaagccc
    11221 tgaccggcaa agtcaaagca ggcgatctgg ttaaatttgc agccgaacaa gtcggcggca
    11281 aaggaggcgg ccgtcccgac ttggcgcagg ccggcggcac ggatgccggc aaattgcccg
    11341 aaatgctggt aagcgtggaa agctggcttt gtcagaaatt aagttaaatt taaatcttaa
    11401 taagccattt gagtcttgtt caaatggctt atattttgga ttgttagaat gaatactaaa
    11461 acagagttac aaaaactttt agaagaggat atatcaactt taacagaaac actaatttgt
    11521 gcagatgctc ttcctccaag atatgtacgt agcattgcaa ctcctattgt ccgtcgatgg
    11581 ttaattgaca aacaattaaa tattctcgca aaagaaatag gtttaactat agaattgccc
    11641 atccttgata caagtttggt atttgaaaaa ttgtcaacct tagagaataa agttaatttt
    11701 tatatggctg gtggagttta tttaggcgga gaatttatta gctctatata tcattcatca
    11761 caagaatttt ctggtgaacc aataatctat gctgaaccaa atataatttt atgtcctgct
    11821 gaaaaatttt taactttaaa acgagttttc cataatggaa atatttttaa tatgaatcag
    11881 ataattactt ttttatccaa taaacaagga ggagtacatt ttgataaaaa ttacgataaa
    11941 tataaaactt gacaagttgc tatagaaaaa gcagcaaatt ttttaaaact cggtaatcca
    12001 tataatgaag ataaattatc tcttagtgaa gaacatgata ctattttagt agttttacca
    12061 ttagaaaaag gatatgaatg gaattgtctt gagatagaag tactatccgc tgctcaatct
    12121 ctagcaaata tctattgcaa caaagttcgc ctcatagatg gtcatgtttg gaaggaataa
    12181 aagaaaaagc atagttggta atttttagtt acgtagggtg ggcttcagcc caccgttttt
    12241 tcatttttga ttgttgattg gtgggctaaa gcccacccta cggttactaa aatcccgtca
    12301 ttacctcgca ggtgggaatc cggtttgttc ggtttcggtt ttttagggac agtgccgcaa
    12361 cattaaattt ccagattccc actttcgcac aaataacagc gcaaataggg caaagataag
    12421 gatatgggtt tttttctttg aatttactat acaattcgcc cgtccccgtt atagaaaccc
    12481 aagccgatga aaaccctgct ccaactccaa accgccgcac aaaacttcgc cgcctactac
    12541 aaagaccaaa ccgacgaacg ccgcgagaaa gacaccttct agaacgaatt tttcgccatt
    12601 ttcggcatcg accgcaaaaa cgtcgcccac ttcgaatacc ccgtcaaaga ccctgccgac
    12661 aacacccaat tcgtcgatat attttgggaa ggcatcttcc ttgccgaaca caaatccgcc
    12721 aacaaaaacc tgaccaaggc caaagagcag gcggaacgtt atttacagga aatcgggcgc
    12781 accaagccct ccgcgctgcc cgaatattac gccgtcagcg attttgccca tttccacctt
    12841 taccgccgcg tacctgaaga aggcgcagaa aaccaatggc agttcccttt ggaagaattg
    12901 cctgaataca tcacgcgcgg cgttttcgac ttcatgttcg gcatcgaagc caaagtccgc
    12961 caaattcaag aagaagccaa cattcaagcg gcggcgacca tcggcaggct gcacgacgcg
    13021 ctcaaagaag aaggcattta cgaagaacac gagctgcgcc tcttcatcac gcgcctgctt
    13081 ttcctctttt ttgccgacga cagcgccgtt ttccggcgca actacctttt ccaagacttt
    13141 ttagaaaact gcaaagaagc cgacacgctc ggcgacaagc tcaatcaact ctttgaattt
    13201 ctcaacacac ccgaccaaaa gcgcagcaag acccaaagcg aaaaatttaa aggtttcgaa
    13261 tacgtcaacg gcggtctttt caaagaacgc ctgcgcactt tcgacttcac tgccaagcag
    13321 caccgcgcct taatcgactg cggcaatttc gactggcgca acatcagtcc agaaatcttc
    13381 ggcacgctct tccaatccgt catggacgcg caagagcggc gcgaagcggg cgcgcactac
    13441 accgaagccg ccaatatcga caaagtcatc aacggccttt ttttagaaaa cctgcgtgcc
    13501 gaatttgaag ccgtcaaagc cctcaaacgc gacaaagcca aaaaactcgc cgccttctac
    13561 caaaaaatcc aaaacctgca attcctcgac cctgcctgcg gctgcggcaa cttccttatc
    13621 gtcgcctacg accgcatccg cgcccttgaa gacgacatca tcgccgaagc cctcaaagac
    13681 aaagcagacg gcctgttcga cagcccgtcc gtccaatgcc gtctgaaaca gtttcacggc
    13741 atcgaaatag acgaatttgc cgtcctcatc gcccgcaccg ccatgtggct caaaaaccac
    13801 caatgcaaca tccgcacaca aatccgcttc gacggcgaag tcgcctgcca tacgctgccg
    13861 ctcgaagacg ccgccgaaat catccacgcc aacagcctcc gcacaccttg gcaggcggcg
    13921 gactacatct tcggcaatcc cccctttatc ggctcgacct accaaaccaa agagcagaaa
    13981 aacgacctcg aaagcatctg cggccatatc aaaggctacg gcctgttgga ttacgtctgc
    14041 aactggtacg tcaaagccgc aggcatcatg gcgcagcatc cccaagttca gacggcattt
    14101 gtttccacca attccatctg ccaaggccag caggtcgaaa tcctctgggg cagcctttta
    14161 aaccaaggca tcgaaatcca ctttgcccac cgcaccttcc aatggacgag ccaagccgca
    14221 ggcaaagccg ccgtccactg catcatcgtc ggcttccgcc aaaagccgcc aatgccgtct
    14281 gaaaaaaccc tctacgacta tcccgacatc aaaggcgaac ccgaaaaaca cgccgtagcc
    14341 aacatcaatc cttatctgat cgatgcgccc gatttgatta tcgccaagcg cagccgtccc
    14401 atacattgcg aacctgatat ggtcaacgga agcaaaccga ccgaaggcgg caaccttatc
    14461 ctttcaaccg ccgaaaaaga tgccctgatt gccgccgaac ccttggcgga gcaatacatc
    14521 cgccccttta tcggcgcgga tgagtttctc aacggcaaaa cccgttggtg cctgtggttt
    14581 cacggcgtat ccgatgtcaa acgcaaccac gacctgaaac aaatgcccca agttcaagcc
    14641 cgtattcagg cggtcaaaac catgcgcgaa gccagcagcg acaaacaaac tcaaaaagat
    14701 gcagcaaccc cgtggctttt tcaaaaaatc cgccagcctt cagacggcaa ttatctgatt
    14761 attccgagcg tgtcgtctga aagccgccgt ttcatcccca tcggttatct gtcgtttgaa
    14821 acagttgtca gcaatctggc atttatcctt ccaaacgcca ccctctacca cttcggcatc
    14881 ctcagctcca ccatgcacaa cgcctttatg cgtaccgttg caggtcgtct gaaaagcgat
    14941 tatcgctact ctaataccgt cgtgtacaac aacttcccct tccccgaaag ctgccggttg
    15001 ccgtctgaaa acgaccgccc cgacccgctc cgcgccgccg tcgaagccgc cgcccaaacc
    15061 gtcctcgacg cgcgcggaca ataccgccga gaagcgcagg aagccggttt gcccgagccg
    15121 accctcgccg aactctatgc gcccgacgca ggctataccg ccctcgacaa agcccacgcc
    15181 accctcgaca aggcagtcga taaagcctac ggctacaaaa caggcaaaaa taccgacgac
    15241 gaggcagaac gcgtcgcctt cctgttcgag ctgtaccgca aggcggcggc aattgcgtag
    15301 tagttgcgta gtagttgcgt aggatagttg cgtagggtgg gcttcagccc accaactccc
    15361 accaactccc accatcttca ccctaaaacc ccgtcatctc cgcaaaagca attgcgtagg
    15421 gtgggcttca gcccaccgtt tttcttcatt ttcggttgtt gattggcagg ctgaagccca
    15481 ccctgcggtt acaaaaatcc cgtcattccc gcgcaggcgg gaatccagac ctttcggggc
    15541 ggaaacttat cggataaaac gatttcccct gcatcctgga ttcccacttt catgggaatg
    15601 acgggatata ggtttcccta cggacgtgtt cagattcccg ccttcgcggg aatgacggcg
    15661 gagaagtttc tgtctttccg ataacttacc gtaacttaaa atcccgtcat taccgcaaaa
    15721 gcagttgcta cggttacaaa aatcccgtca tccccacgaa agcgaaaatc ctgcctgtcg
    15781 gatttcggtt ttttcgggcg tttcgggaac ttataaatcg tcattcccgc gcaggcggga
    15841 atccagaacg cgggacggcg gcaatattca aaggt
//
LOCUS       DAWFKN010000046        15148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFKN010000046 DAWFKN010000000
VERSION     DAWFKN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.046"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            306..1460
                     /gene="obgE"
                     /locus_tag="WGR46_001349"
     CDS             306..1460
                     /gene="obgE"
                     /locus_tag="WGR46_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0856280.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1543..2592
                     /locus_tag="WGR46_001350"
     CDS             1543..2592
                     /locus_tag="WGR46_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0856281.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3280..4701
                     /locus_tag="WGR46_001351"
     CDS             3280..4701
                     /locus_tag="WGR46_001351"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0856282.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGVASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4784..5563
                     /gene="xth"
                     /locus_tag="WGR46_001352"
     CDS             4784..5563
                     /gene="xth"
                     /locus_tag="WGR46_001352"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0856283.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIATSGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5550..5894
                     /locus_tag="WGR46_001353"
     CDS             5550..5894
                     /locus_tag="WGR46_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856284.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            5904..6434
                     /locus_tag="WGR46_001354"
     CDS             5904..6434
                     /locus_tag="WGR46_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0856285.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWRAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6527..7642)
                     /gene="asd"
                     /locus_tag="WGR46_001355"
     CDS             complement(6527..7642)
                     /gene="asd"
                     /locus_tag="WGR46_001355"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0856286.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAKVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7818..8399
                     /locus_tag="WGR46_001356"
     CDS             7818..8399
                     /locus_tag="WGR46_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0856287.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8461..9315)
                     /gene="folD"
                     /locus_tag="WGR46_001357"
     CDS             complement(8461..9315)
                     /gene="folD"
                     /locus_tag="WGR46_001357"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0856288.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9538..9615
                     /locus_tag="WGR46_001358"
     tRNA            9538..9615
                     /locus_tag="WGR46_001358"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9573..9575,aa:Pro,seq:tgg)
     gene            9640..9716
                     /locus_tag="WGR46_001359"
     tRNA            9640..9716
                     /locus_tag="WGR46_001359"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9674..9676,aa:Arg,seq:tct)
     gene            9744..9819
                     /locus_tag="WGR46_001360"
     tRNA            9744..9819
                     /locus_tag="WGR46_001360"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9777..9779,aa:His,seq:gtg)
     gene            9954..10460
                     /locus_tag="WGR46_001361"
     CDS             9954..10460
                     /locus_tag="WGR46_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0856289.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10457..12235
                     /locus_tag="WGR46_001362"
     CDS             10457..12235
                     /locus_tag="WGR46_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0856290.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDEQGVLHLETAEGKQTVVSGEISLRPDNRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12348..13172
                     /locus_tag="WGR46_001363"
     CDS             12348..13172
                     /locus_tag="WGR46_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0856291.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(13935..14723)
                     /locus_tag="WGR46_001364"
     CDS             complement(13935..14723)
                     /locus_tag="WGR46_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0856292.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(14937..15131)
                     /gene="thiS"
                     /locus_tag="WGR46_001365"
     CDS             complement(14937..15131)
                     /gene="thiS"
                     /locus_tag="WGR46_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0856293.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3633 a         3837 c         4185 g         3493 t
ORIGIN      
        1 cgagtttatc ctagttatct aggacagccc caaaacttat tatatgtaac ggtttgaacg
       61 gacattccgt cgggtcggaa tgtcaaaaag gcgcgattgt accaaagaag gtggacataa
      121 ttttgtttgc aggctgaaga tttgcttaaa aattcattaa gatgggcgga acaaatagtt
      181 tgggtacggc gtgttgaaat accgcttatc ccttataata gcgtccgaaa ttctgttcgg
      241 acggcatcaa aacacacgcc atgccgtctg aacccccatt tgaaaccaac aaacaagagc
      301 attgaatgaa attcatcgac gaagcaaaaa tcgaagtcgc cgcaggcaaa ggcggtaatg
      361 gcgcaaccag tttccgccgc gaaaaattcg taccgcgcgg cggcccggac ggcggcgacg
      421 gcggcaaagg cggcagcgtt tgggcagaag ccgacgaaaa caccaacacc ctcgtcgaat
      481 accgcttcgt caaacgctac caagccaaaa acggcgaaaa aggccacggt tccgaccgtt
      541 acggcgcagg tgcggacgac atcgtcctca aaatgcccgt cggcaccctt atccgcgacc
      601 tcgacaccgg cgaaaacgtt gccgacctca cttatcacgg acagcgcgtc tgccttgcca
      661 aaggcggcaa aggcggcttg ggcaacatcc acttcaaatc gtccgtcaac cgcgccccga
      721 aacaatccac ccccggcgaa gaaggcgaag cccgttccct gcaactcgaa ctcaaagtcc
      781 tcgccgatgt cggcttattg ggtatgccca acgccggcaa atccaccctg attaccgccg
      841 tatccgccgc acgccccaaa atcgccaact accccttcac caccctgcat ccaaacttag
      901 gcgtggtacg catcgacgaa aaccacagct tcgtgatggc cgacatcccc ggcctgattg
      961 aaggcgcggc agaaggcgca ggcctcggcc atcgtttcct caaacactta tcacgtaccg
     1021 gcctgctgct gcacgtcgtc gatttggcgc ccttcgacga aaccgtcaac cctgccgaag
     1081 aagcacttgc tatcatcaac gaattgcgca aatacgatga agaactttac ggcaaaccgc
     1141 gctggctggt gctgaataaa ctcgacatgc ttgacgaaga agaagcccaa acgcgcactg
     1201 ccgccttcct cgaagccgtc ggctgggact accccaaacc cgacgaccgt ttccaatttg
     1261 acatggaaac cccgcgcctc ttccaaatca gcgcgctgac ccaccaaggc acgcaggaat
     1321 tggtacacca aatcaaccaa tacctgaccg agaaaaaacg tatcgaagct gaaaaagccg
     1381 aagcggagaa agcagcggca aatgttgaaa ttatcgaaca gcagcctaaa acggatacgg
     1441 gcgtgtttaa gccggagtga aaaaagaaag ggtttcagac gatatttgtt ctacaaaagg
     1501 ttgtttgaaa caattttaaa gcaagattga aatggattaa aaatgtcatc taaccaatat
     1561 atccagtcgc ttgagttgac aaactttacc gttcttccaa acgatacttt tgaattttct
     1621 gaaaatctca atgttattgt tgccgaaaac ggttgtggca aaacacattt gttaaaaatt
     1681 ttatacagcc tgctagaagt aacatctaac accaaaaaca gattactgaa aacagaattg
     1741 caaaaaagtt ttgccgataa attactgaat gttttccgtc ccgattcctt gggcaggttg
     1801 agcaaacgtt tgcaggggcg cgggcgtacg gaaatcgttt taaaactgca aaacggcact
     1861 acgcatagta gtctgaattt ttccagtaac tcatcatcgc aagtcaatgt tcaaagcatc
     1921 ggattgaaag aaaatgaata tactccgaca cctatcttct tacccagccg cgaattaatt
     1981 acactttgcc cttggttcac ttctttgtat caaaaccaaa gcatcccttt cgaagaaact
     2041 tggtttgata cctgtatgca attaaatcac ccgcttgcca aaggccccag agaaacaaaa
     2101 attcgagaat tgctcgaacc tattgagaac gcgatgggag gaaaagtatc tgaagaacaa
     2161 ggaaggtttt atctatcgct gacaaatacg ggcggtaaga ttgaagcacc tttggtagca
     2221 gagggtttgc ggaaatttgt catgattgcc cggttaatcg caacaggggc attgctggat
     2281 aaaggctatc tattttggga tgaaccggaa gctaatctga atcctaaatt gatcaaagtg
     2341 gctgcccgta ttatttggtc tttatctcaa cagggtattc aggtttttat tgcaacacac
     2401 tctctattct tattgagaga attggaactt ttgaagcttg aaaataacac ggatttacct
     2461 gtacgttttt tctcgttgat tgcttccgat gatggaacaa aagtggagca agggaacagt
     2521 attaatgatt tgaacacatt gatattgtta gatgaaaaca ttatgcaggc agacaggtat
     2581 ctgaatattt gaggttaagc tatgactaca atcacagaaa agagattaac ctttgctttt
     2641 cctgaagatt attacgttac caagtatgac gaatgggaac attataaaat ttttcagaac
     2701 agttgtaatt tacgtaataa aattgataca aatgaaaagg gaaagaacgg gataaatcaa
     2761 agtgtagatg acgataatgg cagctcaggt gtagatataa tagcccttca tgagtcgaca
     2821 ctttggctaa tcgaaattaa agattattac cgacttgggc ttgagccaaa tgcccaatca
     2881 atagatgaaa aactttctga cctaccatat ttaatcgcaa gaaaaattcg agattcattg
     2941 gcaggattgg tttctgctaa atttaaagca gaaaaacagg aagaaaaaga tttttctcgc
     3001 ttggctttag attgtaacga aataaaaata gtccttcata ttgaaatgcc tagtataaga
     3061 agtaaacttt atccctcgtc atcagattta gcaaacttat taaaagataa gtttaaatta
     3121 tcagaattta caaaaaattt tgcaaactgt tatgcagaac caattttcac aaatatcagc
     3181 catattaata atccgcaact acgtaatgtt ccttggtccg tatctacagg aacagaacag
     3241 aaattatcat ccgagcaaca aagattaatc cataatccca tgaccaccat ctacaacacc
     3301 cttacccgcc aaaaagaacc ctttgccccc atcgaccctg aaaacgtgcg tatgtacgtt
     3361 tgcggcatga ctgtttacga ttattgccac ttaggccatg cccgtgtgat ggttgtgttc
     3421 gacatgattg cccgttggtt gcgtgagtgc ggctatccgc tcacttatgt acgcaacatc
     3481 accgacatcg acgacaaaat cattgcccgt gcggctgaaa acggcgagac catcggcgag
     3541 ctgactgcgc gtttcattca ggctatgcac gaagatgccg atgctttggg tgtgttgcgt
     3601 cccgatatcg agccgaaggc gacggaaaac attccgcaaa tgattgccat gattgaaacc
     3661 ctgattcaaa acggcaaggc atatcctgcc gcaaatggcg acgtttacta cgccgtgcgt
     3721 gagtttgccg cttacggaca actgtcgggc aaatcgttgg acgacctgcg cgcgggcgaa
     3781 cgcgtggaag tggacggttt caaacgcgat ccgcttgatt ttgtattgtg gaaagcggcg
     3841 aaagcaggcg aaccggcgtg ggaaagcccg tggggcaacg gccgtccggg ttggcacatt
     3901 gaatgctctg ccatgagtga aaacctgttc ggcgatacct tcgacatcca cggcggcggc
     3961 gcggatttgc aattcccgca tcatgaaaac gaaatcgccc aaagcgtcgg tgccagcggt
     4021 catacctgcg gccacgacca cgcacaaacc caccacggtc aaagcattgc cagccacgtc
     4081 aaatattggc tgcacaacgg ctttatccgc gtggacggcg aaaaaatgtc caaatcactg
     4141 ggcaacttct tcaccatccg cgaagtgttg aaacaatacg accccgaagt cgtgcgcttc
     4201 ttcatcctgc gcgcccacta ccgcagcccg ttgaactatt ccgacgcgca tttggacgac
     4261 gcaaaaggcg cgttgacgcg tttgtatacg actttgaaaa atacgccgcc cgccgatccg
     4321 atgccgtctg aagccggcga tgactacacg cgccgctttt atgttgcgat gaatgatgat
     4381 tttgatacgg tgaaagcggt ggcggtgttg ttcgaacttg ccggcgaagt gaacaaaacc
     4441 aataacgcgc aactggcagg ccgtctgaag gctttgggcg gcatcatcgg actgctgcaa
     4501 cgcgatccga ccgagttcct gcaaggcggc gtggcttcag acggtctctc caacgaagaa
     4561 atcgaagact tgatcgcccg ccgcaaacag gcgcgctccg ataaaaactg ggcggaatcc
     4621 gaccgcatcc gcgaccttct gaacgaacac aaaatcattt tggaagacaa cgccggcggc
     4681 acgacttggc ggcgcggtta aaatccatcg aaaagggtaa atgtgccgca ttttgccctt
     4741 ttttgttcag acggcatttt tattgtgaga acttattttt attatgctta aaatcatttc
     4801 cgccaacgtc aacggcatcc gctccgccta caaaaaaggt ttttacgaat acatcgccac
     4861 atcgggcgcg gacattgtct gcgtgcagga actcaaagcg caggaggctg atttgtctgc
     4921 cgatatgaaa aatccgcacg ggatgcacgg ccattggcat tgcgccgaga agcgcggtta
     4981 cagcggcgtg gcggtgtaca gcaaacgcaa acccgacaat gtgcaaatcg gtatgggcat
     5041 tgaagaattc gacagggaag ggcggtttgt gcgttgcgat ttcgacaggt tgagcgttat
     5101 ttcgctttac ttgcccagcg gcagcagcgc ggaagaacgc cagcaggtga aataccgttt
     5161 ccttgatgcg ttttacccta tgctcgaagc gatgaaaaac gaaggacgcg acattgtcgt
     5221 ttgcggcgac tggaacatcg cccaccaaaa catcgacctg aaaaactgga aaggcaatca
     5281 gaaaaattcg ggcttcctac cggaagaacg cgagtggata ggcaaggtta tccacaagct
     5341 cggttggacg gatatgtggc gcacgcttta tcccgatgtg ccgggctaca cgtggtggag
     5401 caaccgcggg caggcgtatg cgaaagatgt cgggtggcgc atcgattatc agatggttac
     5461 gcccgaactt gccgccaaag ccgtgtccgc acacgtttat aaagatgaaa aattttccga
     5521 ccatgcgccg ctggtcatgg agtatgacta tgctgccgaa taaagttttg ggtaaatatg
     5581 attggaatgt ggacggaaaa acaggaatag gcgcagcatg ggttgtcgct gcgtttatcc
     5641 tgcccatatt ggtgtgggcg atatttatgt tgtcgcggat gcaaggctgg cttgcgccga
     5701 ccaaggcaaa cccgacatgg gcgttggtgt ggctgctgat ctgcctgccc tgcctgctga
     5761 ttgcgtccaa atgtttgggt tggaaaggct ggcggctgtt ggcaaacgtt cttgcctgcc
     5821 tcgccgtgtg cgctctgttg agtgtgcctg ccgcgctgct gattgcgttc acgctgcaag
     5881 acctgctcaa ataggcgcgg acaatggaaa gtttcgagct tgaggatttg acgctgtggc
     5941 tggtacgcga tgcggatgaa gcggaaatgt ggatagaccg ctgggcgatc agttatcccg
     6001 tcgtgcagat gtccgaagcg tcggccggtc aaagcatagg ggaatggcgg gcagggcttc
     6061 agacggcatt tgaacgcata cgcggcaaat acgtcgccgt tgtcgcacac ggtgcgggcg
     6121 cggccgcatt tttggcgtgg ctgtatcagg cagacatcct gacacggaag aaaattgcca
     6181 acatcatcct tgtaccgcag cgtcccgata tttttcccga cgatgcggaa cacactttcc
     6241 aacgcgtccg ctgtccctgc cgtgccgcat tggttgtacc cgaacacggc ggcgtgccgc
     6301 acggttgggc gcaaaaacag gcggatttgt ggaacgcccg cctgttggtt tccccgcatt
     6361 cgggcagttt gaacggcatg ctcggcggct ggcagtgggg tatgaagctg atgcaggaaa
     6421 tgttgctggc gtgaacgctt gccttatcgg aatttcggtt aactggggtt gcaaggaaaa
     6481 tgccgtctga aacaggcttc agacggcatt tccattcaaa cagggcttac aggctgccca
     6541 acacgatacg cagcacgcgg cgcaacggct cggcagcccc ccacaagagt tggtcgccga
     6601 cagtgaacgc gctgatgtat tcgccgccca tctccagttt gcggatacgg ccgacaggaa
     6661 cagacagtgt gccggtaact ttggcaggag tcagctcgtg tatgctggct tctttttcgt
     6721 tagggatgac tttcacccag tcgtttgcac ctgccaaaat cgcttcgatt tcggaaacag
     6781 gcaggtcttt tttcagcttc agggtgaggg cttggctgtg gcagcgcata gagccgatgc
     6841 gtacgcataa gccgtcgatt acggtcggat tgtcgctgcg gccgaggatt ttgttggttt
     6901 ccacaccgcc tttccattct tctttagatt gaccgttgcc caaatccacg tcaatccacg
     6961 gaatcaggct gccggcgagc ggtacgccga agttggcttt cggatagtct tcgctgcgca
     7021 ggaaatcgga cactttgcgg tcgatgtcga gaatcgcgct ggaaggatcg gcaagctcgt
     7081 ccgctacttt ggcgtgaatc gcgcccatgc cgctgatgag ttcgcgcatg tttttcgcac
     7141 ccgcaccgga agcggcttgg taggtcatgc tggttgccca ttcgaccaaa tcgttttgga
     7201 acagaccgcc caaagccatc agcatcaggg aaacggtaca gttgccgccg atgtagtttt
     7261 tcacgccgtt tttgaggccg ttgtcgatga cgttgcggtt gaccgggtcg aggacgataa
     7321 tcgcatcgtc tttcatgcgc agggatgacg ccgcgtcaac ccagtagccg ttccagccgc
     7381 tgtcgcgcag gggttggaag acggatttgg tgtaatcgcc gccctgacag gtaacgatga
     7441 tgtccatttt tgccaattcg gcaacatcgt tggcatccaa caatgttttg gctgcctgac
     7501 cgaaatcagg ggctgcgccg ccgacgttgg aagtggtaaa gaaaaacgct tcaggaatgt
     7561 gggcgaagtc gttttcttct ttcatacgct gcatcaaaac cgaaccgacc ataccgcgcc
     7621 agccgacgaa acctactttc attgtgttgc tccttgagga aaaatcgtta ataaaattat
     7681 gtatggcgta aaggaagcgt tttaacgccg tattgggaaa atgtaaagtt ctttatcggg
     7741 ctttctttaa gtattggcgt tttcggtgtc gtggcggtag aatcggggtc agttaagaac
     7801 aattatcagg aacggatatg gaagaaaaaa acgactacac cgacgcggca tccgacaacc
     7861 gcaacgggca ggaaattgaa gtcggaattg ccggcgcagg cgaccggata cttgccgcgc
     7921 tgctgaacca attgtttacc tttttggttt tgttggtgcc gtttgccgga ctgattgctt
     7981 tcgccatcaa gaatgaaggc aggattggcg gcagtgagga aatattcggg ctgcttttgg
     8041 gcatgacttc cttttgggta ggactggcgg gcattttggt ttataccgtc gttcaaatct
     8101 actatatgag tcgggacggg cagtcattgg gtaagaagat catgagaatc agggtgttga
     8161 aaaccgacgg ccgcaatccg ggttttgtcg gcacggtttt ggtacgcgaa atcgcatggt
     8221 cggttttggt tgccattatt gccgccgtta tcggtcttgc agtaggtgac aacggagaaa
     8281 acgccatcaa cctgctggca ttccttgcca actttgtcct gctctttatg gtcaaacgcg
     8341 accgccgcac gctttacgac atactggcgg atacggttgt cgtcaagctg cccaaataaa
     8401 cggacacggc aaaatgccgt ctgaaagcct ttcagacggt atttttatct tcagaaccgc
     8461 tcaagcatcg tgcagtgaag ccgcgtgcag ggtgttttcc atcaatgtgg caatcgtcat
     8521 cggacccacg ccgccgggaa cgggcgtaat catcgccgcc cgttcttttg ccgtttcaaa
     8581 ttccacgtcg ccgcacaggc tgccatcgtc caaacggttg atgcccacat caataacgac
     8641 cgcgccaggt ttgatccatt cgcctttgac aaagttcgga atgcctacgc cgaccaccaa
     8701 aatatcggct ccggcaacct cgtctgtcag attttcggtt gcgctgtggc agaccgttac
     8761 cgttgcgcgc gccagcagca gttccaaagc ctgcgggcgg ccgacgatat tcgacgcgcc
     8821 gaccacgacc gcttttttcc ccttcggatc aatgccgtaa gcttccaaaa gcgtcatcac
     8881 gcccttgggc gtacacgggc gcatcagcgg cattttgacc gccagcctgc cgacattgta
     8941 aggatggaag ccgtccacgt ccttatccgg cgaaatacgt tccaaaaccg cctggctgtc
     9001 gaggtgcttc ggcagcggta gctgaaccag aataccgtcc acttcggaat cggcattcag
     9061 gcggtcgacc agtgccagca gttcttcctg cgatgttgat tcgggcagct cgtaagacag
     9121 tgatttgatg ccgcattttt ggcaggcggt tttcttgttg cggacataaa ccgcgctggc
     9181 agggtcgcct ccgaccaaaa ccacggccag gcaaggcatg tgcagattgt cctgttggcg
     9241 ttgcgccacc gcttcggcaa ccgcctgcag gcgtttttgc gaaacttctt taccattgat
     9301 cagttgtgcc gacatatccg tccttttgtt aaacaggtag aaaatgctga aaattatcgc
     9361 attttccgtt tgtttttaca gcaggatgcc gaaaataaat ttgccctgaa ataaaatacg
     9421 gctgtttgga cggattaaat attttttcaa atcaaaaaac tatatttttc atcttggtgt
     9481 cttgaaaata aatattgacg catctttccc tgagttgtat agttcgcttc ttcaagtcgg
     9541 ggcgtagcgc agcccggtta gcgcatctgc tttgggagca gagggtcgtg agttcgaatc
     9601 ccaccgcccc gaccaatcaa tcagcacgaa tcggcatgag cgcccgtagc tcaaccggat
     9661 agagcaccga ccttctaagt cgggggttac aggttcgatt cctgtcgggc gtgccaagaa
     9721 tttgattgaa aaccgttttt atggtggctg tagctcagtt ggtagagccc cggattgtga
     9781 ttccggttgt cgtgggttcg agccccatca gccaccccag ataaaggccc gtacttttcg
     9841 gtacgggtct tttctcgttt gcgcgtactg tccgcgccgc cggctttgcc tttcgcatat
     9901 gcttctgtat catgtaggtt ttctgtcttt tatggttttt gtcggagact ttgatggttg
     9961 acgcttggtc tgttcctgat tttgaatcga aaatctgtcc gcccgaggcg ttggcggcgc
    10021 gtttggcgtt gttgccgcgc ccgctggtgt ttaccaacgg ctgtttcgac atcctccaca
    10081 gggggcacgt tacttatctg gcgcaggcgc gttcgatggg gggcgcgttg gtgctggcgt
    10141 tgaataccga tgcttcggtg cggcgtttgg gcaagggcgg cgaccgcccg attaatcctt
    10201 tggagaaccg tgccgccgtt gccgccgcgt tggaaagtgt ggatttggta acgtggtttg
    10261 acggggatac gccggcggcg ttgattgagg cggtcaaacc tgagattttg gtcaagggcg
    10321 gcgattgggc tgcggataag attgtcggtg cggcagaaac gttggcgcgc ggcggtcagg
    10381 tgttttcaat tccgtttctg caccagactt cgacaacgaa gactttggca aaaatccgtg
    10441 cggcagaggg cggaaaatga cggttttgaa gccttcgcac tggcgggtgt tggcggagct
    10501 tgccgacggt ttgccgcaac acgtctcgca actggcgcgt atggcggata tgaagccgca
    10561 gcagctcaac ggtttttggc agcagatgcc ggcgcacata cgcgggctgt tgcgccaaca
    10621 cgacggctat tggcggctgg tgcgcccatt ggcggttttc gatgccgaag gtttgcgcga
    10681 gctgggggaa aggtcgggtt ttcagacggc attgaagcac gagtgcgcgt ccagcaacga
    10741 cgagatactg gaattggcgc ggattgcgcc ggacaaggcg cacaaaacca tatgtgtgac
    10801 ccacctgcaa agtaagggca gggggcggca ggggcggaag tggtcgcacc gtttgggcga
    10861 gtgtctgatg ttcagttttg gctgggtgtt tgaccggccg cagtatgagt tgggttcgct
    10921 gtcgcctgtt gcggcagtgg cgtgccggcg cgccttgtcg cgtttgggtt tgaaaacgca
    10981 aatcaagtgg ccaaacgatt tggtcgtcgg acgcgacaaa ttgggcggca ttctgattga
    11041 aacggtcagg acgggcggca aaacggttgc cgtggtcggt atcggcatca atttcgtgct
    11101 gcccaaggaa gtggaaaacg ccgcttccgt gcaatcgctg tttcagacgg catcgcggcg
    11161 gggaaatgcc gatgccgccg tgttgctgga aacgctgttg gcggaacttg atgcggtgtt
    11221 gttgcaatat gcgcgggacg gatttgcgcc ttttgtggcg gaatatcagg ctgccaaccg
    11281 cgaccacggc aaggcggtat tgctgttgcg cgacggcgaa accgtgttcg aaggcacggt
    11341 taaaggcgtg gacgaacaag gcgttctgca cttggaaacg gcagagggca aacagacggt
    11401 cgtcagcggc gaaatcagcc tgcggcccga caacaggccg gtttccgtgc cgaagcggcg
    11461 ggattcggaa cgttttctgc tgttggacgg cggcaacagc cggctcaagt gggcgtgggt
    11521 ggaaaacggc acgttcgcaa ccgtcggtag cgcgccgtac cgcgatttgt cgcctttggg
    11581 cgcggagtgg gcggaaaagg cggatggaaa tgtccgcatc gtcggttgcg ccgtgtgcgg
    11641 agaattcaaa aaggcacaag tgcaggaaca gctcgcccga aaaatcgagt ggctgccgtc
    11701 ttccgcacag gctttgggca tacgcaacca ctaccgccac cccgaagaac acggttccga
    11761 ccgctggttc aacgccttgg gcagccgccg cttcagccgc aacgcctgcg tcgtcgtcag
    11821 ttgcggcacg gcggtaacgg ttgacgcgct caccgatgac ggacattatc tcgggggaac
    11881 catcatgccc ggtttccacc tgatgaaaga atcgctcgcc gtccgaaccg ccaacctcaa
    11941 ccggcacgcc ggtaagcgtt atcctttccc gaccacaacg ggcaatgccg tcgccagcgg
    12001 catgatggat gcggtttgcg gctcggttat gatgatgcac gggcgtttga aagaaaaaac
    12061 cggggcgggc aagcctgtcg atgtcatcat taccggcggc ggcgcggcaa aagttgccga
    12121 agccctgccg cctgcatttt tggcggaaaa taccgtgcgc gtggcggaca acctcgtcat
    12181 tcacgggctg ctgaacctga ttgccgccga aggcggggaa tcggaacata cttaaaaaat
    12241 acaccctttt tcaacacggg gcggtatccg tccgaatccg tccggattct gccgcccgac
    12301 agactgccgc ccaggcgggc ggggtttcaa acattgagga aattctaatg aaatggctct
    12361 tcaccctttt ggcggtactc aatattgccg tattcggcgg cacggtgggc tataaattgg
    12421 ccgttaaagc gacgggcggc gtgccggaaa accgggccgt cgaaaacacg ccccccgcaa
    12481 cgcccgccgc cgacaatgcg gcggcatccg tggaggatac ggcggcactg ctcaaaccgg
    12541 gcgacatcct gaccgaagag caggcggagc agttgcgctt gaagaaagaa gcggaacaga
    12601 aaaaactgag ggagaaaaaa cagcgtgaag aaaaagcccg ccgcgaaaaa ctcgccgccg
    12661 aaaaggcgca ggcggaacgc gaaaacggcg cggcggatgc cttatgcgcc gcgcaggcaa
    12721 gcctcacgat ggacgaagat gactaccacc gcatcaaagg acttttgggc aaatggtcgc
    12781 acgttgccag caggagcgtc gaaaaacgca ccgccaaagc caaacctgcc gacaaaacct
    12841 accgcgtcgt cctgcccgtt tccgccgatg ccgaaaatca ggcggcggag ctgtctgcca
    12901 aaggtttcaa ccccataccg tttgacggcg cattgagttt gggtgtcggc aacagccggg
    12961 aaaacgccca agccctgcaa aaccggcttg ccggcgccgg attcggcggg gcgcatattg
    13021 tcgaacactt tgccgaagcc gacaggcagg acgattcttt gagcgtgtcg cgtatgacgg
    13081 ttttgtttac cggcgtgaat gccgccgatg cggacgaaat ccgtaaaatc acgtccctat
    13141 acggcaaact gaacctcaag tcttgcaaat aggcgcgaaa gccggacgat aaacgccaaa
    13201 cccaccgcga aggtgggttt ggcgtttcgg ttttgagtat ctgatatata aaaagggaat
    13261 gtatgtctcg tatatgccga ctccaagtgt gaaagtgatg atggggaaat atggtgttta
    13321 atggttcgga acggacggct tccgagtggt tttgattata cgctaataat aataattatc
    13381 aataaaaatt taaaaataaa tgcagatttt ttgtaatacg atgaaaaata aacaaattaa
    13441 cctgtccgcc gatgtttgtg attcgggtcg gcaaactttc attgtgtcgg acaaatcggg
    13501 gtcggcagtc atagctgagg gcttcgtgcc tgacggaaat aaggttgccc gtgtttgaac
    13561 atccgtccga ttgggcgcgg acagggttgt cattcggggg tgtttgagcg tggattcggg
    13621 gatgcgaaag cggtaaaacc gttgcggatg aaggcttacg gacggcttta ggtattttta
    13681 agtgtattca aacaacaaca aaacgcttct tctattgccg tatttagggc ttgcgcggca
    13741 gcctgcacac cgtggaggcc gagtgtttcg ttggttgcgg catcgggacg ggcggcaagg
    13801 ttttccggcc ggtttttcca ttttttccga caaagccgtg atgccgggga atggcggttt
    13861 gtccgtttcg gaccgccgcc gttttgagcc gcgatgccgt ctgaaccgtt cagacggcat
    13921 ttttgccgcc tttttcaata ttccgccgaa tgccaaaacg gctgtccgac tgtcggcgtg
    13981 ctggcttgcg ctttgtcgcg tgcttcgacc ggttcggctt caaatgccag ccgtccggat
    14041 tcgacggcga gtgcgaaggc gcgtgccata ttgaccggat cgccgctgcg ggaaacggca
    14101 gtattcaaaa gcacgccgtc aaaaccccat tccatcactt gtgccgcctg tgagggcaaa
    14161 cccaagcccg cgtcgataat cagcggcgtg tcgggcaggc gttcgcgcag gacgttcaac
    14221 gcgtaggcgt gaaccgcgcc caaacccgtg ccgatcggag ccgcccacgg cattaacgcc
    14281 tgacagcccg cgtcgagcag gcggcggcag gcaatcaggt cttcggtgca ataaggcagc
    14341 actttgaagc cgtctttaat caggatttcc gccgcttcga caagttggaa cacatccggc
    14401 tgcaaggtgt cgtcatctcc gatgagttcc aattttatcc aatcggtttc aaacacttcg
    14461 cgcgccattt gcgccgtcgt taccgcttcc tgcacgcttt ggcagcctgc cgtgttcggc
    14521 aggacgggaa cgccggtttc ttgaagcagc gaccaaaacc cctgaccgtg cgcctcgccg
    14581 ccgcttcccg cgcggcgcag cgagacggta atcatcgcag gctgggcggt ttggatggat
    14641 tgtttgagga tttcgggggt cgggtaggcg gctgtgccga gcagcagccg cgaagggaaa
    14701 gtttcgctgt acagggtgag cataatgggt tcctttgtat agtggattaa caaaaaccag
    14761 tacggcgttg cctcgtctta gctcaaagaa aacgattctc taaggtgctg aagcactaag
    14821 tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttaaag
    14881 ttaatccacc ataacgttgt tttggggaca ggggtcgtct gaaaaggcaa aaccgcctag
    14941 ccgccgacca ccggccgcac gatatcgatt ttgtcgtttt cgtttaaaac cgtttccgca
    15001 tacgcgcctt tggggacgaa aacggtgttg accgccacgg caaagggctt ttgcggcgcg
    15061 gtttgggcga tgaggtcggc aacgcttgtg ccgtgaagtt cggcgggtcc gccgtttaag
    15121 atgatgttca tggtttctcg atgtttct
//
LOCUS       DAWFKN010000047        14950 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFKN010000047 DAWFKN010000000
VERSION     DAWFKN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.047"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            136..2016
                     /locus_tag="WGR46_001366"
     CDS             136..2016
                     /locus_tag="WGR46_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0856294.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQVQAADIDTDLLWEVCGGEEFAAEAIAEEYYGHTPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAETLKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPGDKMEQVIMQRLSTVYFPNGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(3250..4275)
                     /locus_tag="WGR46_001367"
     CDS             complement(3250..4275)
                     /locus_tag="WGR46_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0856295.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALK
                     PGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(4380..6788)
                     /locus_tag="WGR46_001368"
     CDS             complement(4380..6788)
                     /locus_tag="WGR46_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0856296.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            6858..7862
                     /locus_tag="WGR46_001369"
     CDS             6858..7862
                     /locus_tag="WGR46_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0856297.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(7919..8617)
                     /locus_tag="WGR46_001370"
     CDS             complement(7919..8617)
                     /locus_tag="WGR46_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0856298.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            complement(8911..9018)
                     /locus_tag="WGR46_001371"
                     /pseudo
     CDS             complement(8911..9018)
                     /locus_tag="WGR46_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9076..10614
                     /gene="murJ"
                     /locus_tag="WGR46_001372"
     CDS             9076..10614
                     /gene="murJ"
                     /locus_tag="WGR46_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0856299.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            10746..11702
                     /locus_tag="WGR46_001373"
     CDS             10746..11702
                     /locus_tag="WGR46_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0856300.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            11753..12535
                     /gene="trpC"
                     /locus_tag="WGR46_001374"
     CDS             11753..12535
                     /gene="trpC"
                     /locus_tag="WGR46_001374"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0856301.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            12601..14901
                     /gene="recQ"
                     /locus_tag="WGR46_001375"
     CDS             12601..14901
                     /gene="recQ"
                     /locus_tag="WGR46_001375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0856302.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLGMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGKTGVMLRPLK
                     RNKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         3149 a         4218 c         4178 g         3405 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctcccc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggtgc aggcggcgga catcgacacc gatttattgt gggaagtatg cggcggagag
      361 gaatttgccg ccgaagccat cgccgaagaa tattacggcc atacgccgac caaaaccgag
      421 ctggcggcga ctttgattgc gctttacgcc gcaccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacaac aggacgcgca aatcgacgct tgggcggaaa ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcttacacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctccgccta cgaactggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg ttttcccgac cttccgcttc ctgaaatgcc cgacctgccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttggg caacggcacg aagcgcgtcg gcatccacat tgccgcgccg
      961 tcgcttgccg ttaaaccggg cgacaaaatg gaacaagtca ttatgcagcg gttgagtacg
     1021 gtttattttc ccaacggcaa aatcactatg ctgcctgaaa actggatagc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcgga aatccagatt cttcggtaca gaaacttatc ggataaaacg
     2281 gtttctttag attctacgtc ctagattccc gcctgcgcgg gaatgacggc ataggggttt
     2341 ccgttttccc gataaattac cacaacccaa aatcccgtca ttcccgtaaa agcgggaatc
     2401 cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt cattcccacg
     2461 caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc cgcgaaagcg
     2521 ggaatccaga ccgttcggct tcagtttttt ggtttcgggc aacttctgca tcgtcattcc
     2581 cacacaggtg ggaatctagg tctgtccgca cgggaactta tgcgccgtca ttcccgcgaa
     2641 agcgggaatc cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt
     2701 cattcccaca caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc
     2761 cgcgaaagcg ggaatccaga cccttcggca cagaaactta tcggataaaa cggttgcttt
     2821 agattctacg tcctagattc ccgctttcgc gggaatgacg gcatagaggt ttctgttttc
     2881 ccgataaatt cctgttactt ttcgttgcta gattcccgcc tacgcgggaa tgacgatgga
     2941 aagattgttg ttgcttcgga taaatttctg cagttttaaa taaccggatt cccgcctgct
     3001 cgggaatgac ggcataggtt tttttgtttt tccgataaat taccacaacc caaaatcccg
     3061 tcattcccgc gacggcggga atccagtcct ttaaactcca gccattcccg ataaattcct
     3121 gttacttttc gtttctagat tcccgctttc gcgggaatga tgaatgacgg tgtaaaagta
     3181 actcgaaatc caaaaagcca taccgcccgg atttttgccg atcggtatgg ctttttgctt
     3241 caaaccgcct tagcggatgt cgacatacgc gccggaatgc aggtcacgca acaggttgac
     3301 ggttgcctgt tcggcttttt gttggaagat gtattgccgc acggaattgc ggatacgttc
     3361 ctgaggtgtg ccggcatcgc gcacttcgtt caatttgatg atatgccagc cgaattgggt
     3421 gcggacgggc gcgccgacct gtccgggttt gagcgcgtgg acggcttctt caaaggcggg
     3481 aaccatcacg ccgtcggcaa accagcccaa atctccgccg ttgcccgcgc tcgcgtcttg
     3541 cgaatattgg cgcgctaggc tggaaaagtc tgtgccgctg cgggcctctc cgtagatttt
     3601 gcggatggtg ctttccgcgc cgacggcggc gttttcgctg tcggctttaa tcaggatgtg
     3661 ttgggcgcgg tattggcgca acggtgcgcc ttcgggcagg gtgatgcctt gtttttgcgc
     3721 ctgctcgagg aaggcatcga tttcagcttc gctcacgcgg ctgttctgca tcactgcctg
     3781 ctggcggact ttttcggcaa tgatgttgtc ggcaaaatcg cggcgttggg cggggctgag
     3841 gtttttgagg gcggaatttt ttgcgacgac ggcatcgatt tccgcttcgc ttgcttgaat
     3901 gttgcggcgt ttgcccgcct gtacaatcag ggattggttg acaagctgca tcagcacctg
     3961 tcgggacagc tcggattcgc ttatctgcgc gtctttgggc aggttggctt tggcttcggc
     4021 aacggcttcg gcaagccggc ggcgcgtgat gacttcgttg tcggcaacgg cggcaatgcc
     4081 gtctgaaaag cggataccgc cctgctgttg tgcgggtgcg gcttcttttt gcgccgtggc
     4141 ggcaactttg gcaactttgg cagctttggc agctttggca gctttggcgg atgcggtttt
     4201 tgccttttgc ggtgcggcgt ggacatcggc tgctgccagc aatgcggcgg caatcatcag
     4261 ggctttgatt ttcatcattc tttccttatc gtttgaatcc tgcctttgat ggcgggtttg
     4321 gggttgtcgg tggaatgccg tcttgtgcgg gaacggtgct cctgccgggt ttccgacggt
     4381 cagggccgtt tgttgcgtcc ggcggaaaga gagtgggcgg ggatatagcc gggaacggcg
     4441 acatccatcc tgtctgcggg gtttctgccg acactgctga ggtctttcaa ctgaagtgag
     4501 aaaaagacag cgtttttgta ggtgttttcg ccggtaacgt agcgttgggc gtacacgccc
     4561 gcgccccagc agccgcaact gcttttgtat tccgcacccg ccagcacctc tatcggtttt
     4621 ttggcttcaa aaccgtagtt gtaacggacg acggccgaca ggttgcgcgt cagcggccat
     4681 tgtgcggaca ggtcgagctg gctgagtttg tcgtaaaaat aggaaccgtc ggacttcagg
     4741 tagatttttt cgttgcgccc gtatttgtag cgggcgttca gcactttgcc ctgtgcggga
     4801 cggtagcttg caccgacggc gtagttctcg gcgcgtttgt cgttttggtt gtagtggatg
     4861 ctgctgtcga ggatgaagcg gctgccgatg ccgctggagg cgaatgccac ccagtcggaa
     4921 cggctgcgcg gttttttgcc gacactgccg tcaagcatga ctgcgtcgtt tttgaagtag
     4981 aatttctgcc cgatgccggc gcggaaacgc tcttcccccg tcgcgccgtc caaaatacgg
     5041 ctttgcacgg cggcggaaag gctgtttgcg gtgttaatcc tgtcgttgcc gtaatagagg
     5101 ttttcacgaa aaagctgccc gtagccgaag ctgctttccg acgaatcgaa attgggcagg
     5161 tcgttttggg atttggcagg aatatagttg tagaacaggc gcggctcgag ggtttgcagg
     5221 actccgccgc cgaacatccg cgtattgcgt tcgaaggtca tgccgctgtc gatgttgacg
     5281 atgggcagag tgcggctgac gcgtcgggct tcttggctgc cgaagcggtt gaggctgtaa
     5341 taggtggcgt gcagtccgag tttgggacgg acgtaacccc agctgttgct gaaatcccat
     5401 ttgatgtcgg gatagacgac gaggcggctg ccgtcttggc ggctgtcgtg gctgaagcgg
     5461 gtaaattggg cggacacgcc gatttgcgcc ctgccggtgt ttttgcgcca atcggcggaa
     5521 aggcgcggca tcagggcata cggtttgtct ttgtagccgc tttggtttgc cagcgtctgg
     5581 tatttcagaa ccgaaaggcc ggcattcagg ctgccgcccg ccgccctgcc gccataatcc
     5641 agccatacac ggcggttgag gttgacgttg ccggcgattt ctttgttgcc gtaaaagtcg
     5701 cggtagtagc cgctgtcgga gacttggttg aaatcgacac ccgcctgaag cgtgtcggaa
     5761 atgtcgtgcc ggtgctgcca tttcgcctga tagcggttat tcctgccgct tttcttgtcg
     5821 tgcggcagcc aggtcaggtc ggactggccg gcataatccg gccgcaggta gcgtacctgc
     5881 ccgtcaaaga ccgcgccgcg ttcgccgatc acgccgggcg cgaacgtggc atcgagattg
     5941 ggggcaaggt tgaaataata gggaacggaa agggaaacgc cgtccgaacc ggcggacagt
     6001 gagggaacga gcaggccgct tttgcggttg ccgtcaagcg ggaagtccgc ccaaggggtg
     6061 tagaaaatgg gaacgccgcc gaacacgaag gcggcgtgtt tggcaacgcc tatgcctttt
     6121 tcccgatcgg cttcgacgga agcggccttg acataccagc cggcatcgcc ggcggaacag
     6181 gtgttgaatt gggtttccgt cagtttgtaa tgcccttcgc ccaacatttc ggcggtgcgg
     6241 ctgacgcttt gcagccgccg tccgccgtgt tcggtttcca tacggacgtt gtgcgcttcc
     6301 ccggtctgct gctcgagatt gtaggtcagg gtttcgcccc gaatcagcgt accgtcctgt
     6361 tggagggcga accggtcgcc tgcggtaacg gtgtcgcccg actggtcgta atccgcccaa
     6421 tcggcattga gggtcgtccg attgcgttcg acgacgacgt tgccttcggc acgcacctgc
     6481 acctgcgact gtccttccat cctgtcggca acaatgcgcg tatagtcttc ggggatggat
     6541 gcttcgccgc tgccttggac ggcggcttcg gttctctcgg ggctgccgct ttcgttactg
     6601 caaaacaggc aggtcgaacc gaggctcagg ctggtaggct gtatgggttc ggacacgctc
     6661 cgaacgcttc ctccggcggt cggattgtcc gtttcctccg ccgcaacggc atcggcggcg
     6721 gcgcaatgcg tgccgaaaca gaagcccaat gccagcacca gtggtttgag tgaaaataaa
     6781 cgagccaaaa tcgcccctca agtcggtttg ccagttagaa tagcgtttat tgtaacctga
     6841 aatgctttag tactgttatg caacggcaaa ccgaactgaa aaattggctt cagaccatct
     6901 atcccgaacg ggacttcgat ctgtccttcg cggcggcgga tgctgatttc cgccgctatt
     6961 tccgtgcaac gttttcagac ggcggcagcg tcgtctgtat ggacgcgccg cccgacaaga
     7021 tgagtgtcgc gccttattta aaagtgcaga aactgtttga catggtcaat gtgccgcaga
     7081 tattgcacgc ggacacggat ttggggtttg tggtattgaa cgacttgggc aatacgacgt
     7141 ttttgaccgc gatgcttcag gaacagggcg aaacggcgca caaagccctg cttttggagg
     7201 ccatcggcga gttggtcgaa ttgcagaagg cgagccgtaa aggggttttg cccgaatatg
     7261 accgtgaaac gatgttgcgc gaaatcaacc tgttcccgga atggtttgtc gcaaaagaat
     7321 tggggcgcga attaacattc aaacaacgcc aactttggca gcaaaccgtc gatacgctgc
     7381 tgccgcccct gttggcgcag cccaaagtct atgtgcaccg cgactttatc gtccgcaacc
     7441 tgatgctgac gcgcggcaga ccgggcgttt tagacttcca agacgcgctt tacggcccga
     7501 tttcctacga tttggtgtcg ctgttgcgcg atgcctttat cgaatgggaa gaagaatttg
     7561 tcttggactt ggttatccgc tactgggaaa aggcgcgggc taccggcttg cccgtccccg
     7621 aggcgtttga cgagttttac cgctggttcg aatggatggg cgtgcagcgg cacttgaagg
     7681 ttgcaggcat cttcgcacgc ctgtactacc gcgacggcaa agacaaatac cgtcccgaaa
     7741 tcccgcgttt cctcaattac ctgcgccgcg tatcgcgccg ttatgccgaa ctcgccccgc
     7801 tctacgcgct cttggtcgaa ctggtcggcg atgaagaact ggaaacgggc tttacgtttt
     7861 aaacccaatc aaaatgccgt ctgaaaacca agtttcagac ggcattttcc aaacgggctt
     7921 agtgcgcggc tttttgttct tcgcgtactt tgtccgccaa aaggtcgatg gtgttcatac
     7981 cggactccca gtcggcaaat tcgactttat atttgccgcc gacgataacc gtgggcgtac
     8041 cgtcgatttg gaaggtttcg gtcagctcct gcattttgtc ggcgcgcgcc tggctttcgg
     8101 gagattcgta agcggcaagg acttttttgc cgtcaaaggc ggtttgttcg cccagccatt
     8161 ttttgaggac ttccggctct tgcagcttga ttttttggtt gaccatcgca tcgaaaatat
     8221 ggctgttcgc cacatctttg ctgtcggcgg cagccatatc gacggcggcg gcgaggcgtg
     8281 cgagcgtcag catttctttc tgccagacga cgtgttcggt acgcaggtac atatcgtctt
     8341 taaaagactt ggcgtgtttg cttaaaacag gttcgaggtg ggcgcagtgc ggacagaaat
     8401 agccgaaaaa ctcaaggact tcgactttgc ctgcctgctg ttggggaatc gggttggcaa
     8461 ggacagtata gttttgccct tcgaccagcc ctgccggggc ggcggctgcc gaagcggcag
     8521 gcgcgctgtc ggcggggacg ctggtttgga ctttgctgtc gcacgcggca agtgcgaaca
     8581 gggcggcaac gccgagggcg aggtgtttgg atttcatacg gctctccgtg atgttggaaa
     8641 cagatgttgg aaatcaatcg gaatccggct attttattgc atttccgcgt gttgatacag
     8701 tttgcctccg gaaaaggacg ttttcgtttg gggaaaccgc ttcggacggc atcaaacccg
     8761 atgccgtctg aagcggtttc tgtcgtacaa tacgcgccgt cgccccagac ggatacggct
     8821 gttggggaac taatgatgtt ccaacacaca ggacggcaca taaagcaccg ccctatgcgt
     8881 tgccctggtt cggaagggtt acaccccttt caaatatagt ggattaaatt taaaccagta
     8941 cagcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     9001 atttaaattt aatccactat caaagtctga tcctgccgcc ctaaagggcg gggtttcaac
     9061 cgaaaaggaa atacgatgaa tatgctggga gctttggtaa aagtcggcag cctgacgatg
     9121 gtgtcgcgcg ttttgggatt tgtgcgcgat acggtcattg cgcgcgcatt cggcgcaggc
     9181 atggcgacgg atgcgttctt tgtcgcgttc aaactgccca acctgcttcg ccgcgtgttt
     9241 gcggaggggg cgtttgccca agcgtttgtg ccgattttgg cggaatataa ggaaacgcgt
     9301 tctaaagagg cgacggaggc ttttatccgc catgtggcgg ggatgctgtc gtttgtactg
     9361 gtcatcgtta ccgcgctggg catacttgcc gcgccttggg tgatttatgt ttccgcaccc
     9421 ggttttgcca aagatgccga caaatttcag ctctctatcg atttgctgcg gattacgttt
     9481 ccttatatct tattgatttc actttcctct tttgtcggct cggtactcaa ttcctatcat
     9541 aaattcagca ttcctgcgtt tacgcccacg ttcctgaacg tgtcgtttat cgtattcgcg
     9601 ctgtttttcg tgccgtattt cgatcctccc gttaccgcgc tggcttgggc ggtttttgtc
     9661 ggcggcattt tgcaactcgg cttccaactg ccctggctgg cgaaactggg ttttttgaaa
     9721 ctgcccaaac tgagtttcaa agatgcggcg gtcaaccgcg tgatgaaaca gatggcgcct
     9781 gcgattttgg gcgtgagcgt ggcgcagatt tctttggtga tcaacacgat tttcgcgtct
     9841 tatctgcaat cgggcagcgt ttcatggatg tattacgccg accgcatgat ggaactgccc
     9901 ggcggcgtgc tgggggcggc actcggtacg attttgctgc cgactttgtc caaacactcg
     9961 gcaaaccaag atacggaaca gttttccgcc ctgctcgact ggggtttgcg cctgtgcatg
    10021 ctgctgacgc tgccggcggc ggtcggaatg gcggtgttgt cgttcccgct ggtggcaacc
    10081 ttgtttatgt accgtgaatt cacgctgttt gacgcgcaga tgacgcaaca cgcgctgatt
    10141 gcctattctt tcggtttaat cggtttgatc atgattaaag tgttggcgcc cggcttctat
    10201 gcgcggcaaa acatcaaaac gcccgtcaaa atcgccatct tcacgctcat ctgcacgcag
    10261 ttgatgaacc ttgcctttat cggcccgttg aaacacgccg ggctttcgct cgccatcggt
    10321 ttgggcgcgt gcatcaatgc cggattgttg ttcttcctgt tacgcagaca cggcatttac
    10381 cggcccggca agggttgggc ggcgttcttg gcgaaaatgc tgctctcgct cgccgtgatg
    10441 ggcggcggac tgtgggcggc gcaggcttgc ctgccgtttg aatgggtacg cgccggcgga
    10501 atgcggaaag cggggcagct ctgcatcctg attgccgtcg gcggcggact gtatttcgca
    10561 tcactggcgg ctttgggctt ccgtccgcgc catttcaaac gcgtggaaaa ctgaccgatg
    10621 ccgatatcct ttttcggcaa acgcttttcc acgcctatat tcgggcgcgc atcccccacg
    10681 tccgcaccag ccggacgggc tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat
    10741 accttatgat tttgacaccg ccggacacac cctttttcct ccgcaacggc aatgccgaca
    10801 cgattgccgc caaattcctg caacgctccg cacctgcata ccgccgcgag ctgcttcccg
    10861 acagcacggg taaaaccaaa accgcctacg acttttcaga cggcatttcg cccgatgcgc
    10921 cgctggtcgt gctgtttcac ggtttggagg gcggcagtgg cagccattac gcggtcgaac
    10981 tgatgctcgc ggtacgcgat cggggttgga acggcgtagt cgtccatttc cgcagctgcg
    11041 gcggcgtagc gaacaccgcc ccggtgttct accacttggg cgataccgcc gaaattgcct
    11101 ttactttgga cacgctcgcc gcgcgttacc gtgaaatata cgccgtcggc gtatcgctgg
    11161 gcggcaacgc gctggcaaaa tatttgggcg aacagggcga aaacgcgctg ccgcaagccg
    11221 ccgccgtcat ctccgcaccc gtcgatgcag aggcggcagg caaccgcttc gacagcggca
    11281 tcacacggct gctctacacg cgctacttcc tccgcacact gatacccaaa gcacggtcgc
    11341 tccaaggttt tcagacggca tttgccgcag ggtgcaaaac actgggcgag tttgacgacc
    11401 gtttcaccgc accgctgcac ggctttgccg atcggcacga ctactaccgc caaacttcct
    11461 gcaaaccgct gctcaaacac gttgccaaac cgctgctcct gctcaatgcc gtcaacgacc
    11521 ccttcctgcc gcccgaagcg ctgccccgcg cagacgaagt gtccgaagcc gttaccctgt
    11581 tccagccgac acacggtggt catgtcggct ttgtcggcag caccggcggc aggctgcacc
    11641 tgcaatggtt gccgcagacc gtcctgtcct atttcgacag cttccgcaca aacaggcgtt
    11701 aacggtttga tgctaatatt ccccctttcc ccagacgaat acggaacacg acatgaccga
    11761 catcctcaat aaaatcctta ccgccaaggc acaggaagtt gccgcccaaa aagccgccgt
    11821 caacgccgaa cacatccgcg cacttgccgc agaagccgcg cccgtccgca gctttatcgg
    11881 ttcgatacgc ggcaaacacc gcctcaacct gcccgccgtc attgccgaaa tcaaaaaggc
    11941 aagcccgagc aaagggttaa tccgtccgga cttccgaccg cccgagattg cacgcgccta
    12001 tgaaaacgcc ggggcggcgt gtttgtccgt actgaccgac gaaccctatt tccaaggttc
    12061 gcccgaatac ctcaaacagg cgcgcgaagc cgtatcgctg cccgtgctgc gcaaagactt
    12121 catcatcgac gaataccagg tttatcaggc gcgcgcctgg ggggcggatg ccgtcctgct
    12181 gattgccgcc gcactggaac gggaacaatt ggaacgcttt gaagcggtgg cgcacgaatt
    12241 gggcatgacc gtcctgctcg agctgcacga tgaaagcgaa ttggaaaaat gccgcaacct
    12301 gaccacgccg ttgtggggtg taaacaaccg caacctgagg acttttgaag tctccctcga
    12361 ccaaaccctg tcgctgctgc ccgcgctgga aggcaaaacc gtcgttaccg aaagcggcat
    12421 tacaggcaag gcggatgtgg aatttatgca atcgcgcggc gtgcatacct tcctgatcgg
    12481 cgaaacgttt atgcgtgccg acaatattga ggcggaagta ggcaaactct tctaaatccc
    12541 gatttcagac ggcattccct ttctttaacc gacacaccct ttaccggaca cggaaccatc
    12601 atgcaccgac cgaccgccaa acagattctg cacgaagtat ttggttatcc cgaatttcgc
    12661 ggcaggcagg aggctgtcat caatactttg gcaggcggcg ggagtttgac ggtgctgatg
    12721 ccgacgggcg ggggcaagtc tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg
    12781 gcggttgtcg tatcgccgct gattgcgctg atgaacgacc aagtggccag cctgcatgtg
    12841 gccgggattg aagcagcggc agtcaacagt ggtacatcgg cagatgaggc gcgcgagatt
    12901 gccgacaagc ttgcccaagg ccgtctgaag ctgctttatg tcgcaccgga acgcctagtt
    12961 accgaccgct ttttacgttt tctcgaccaa caaaccgtca gcctgtttgc cattgatgag
    13021 gcgcattgcg tcagccagtg gggacacgat ttccgccccg aataccaaca gctcggcatg
    13081 cttgccgaac gctatccgaa cgtcccgcgc atcgccctga ccgctaccgc cgatgccgcc
    13141 acgcgcgccg acatcaagca ttatctgcac ttggacgacg cgcccgaatt tgtctccagc
    13201 tttgaccgcc cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa aaaacaactg
    13261 cttgaattca tccgcaaaga aatgacgggg caaagcggca ttgtgtattg cttaagccgc
    13321 aaaaaggttg aagatgtggc gcggtttttg cgtgaaaacg gattaaacgc gattccgtat
    13381 cacgccggtt tgggtatgga cgtgcgtgag gaaaaccaac gccgctttac gcgtgaagac
    13441 aatattatcg tggtggcgac cgtggcgttc ggtatgggca tagacaaacc cgacgtgcgc
    13501 tttgtcgccc atctcgatat gccccagagt gtcgaacatt tctatcagga atcggggcgc
    13561 gccggccggg acgggctgcc tgccgcgagc tggctgtgtt acggcttgaa cgattgggtg
    13621 ttgctgcgcg aacggattgc cgaaggcaac agcggcgaag tgcaaaagca aatcgaaatg
    13681 caaaaactcg atgccatgct tgccgtctgc gaaaccgccg cctgccgccg cgtgctgctg
    13741 ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt gcgacaactg cctgcatccg
    13801 cccgtacggt ttgacggcac ggtgttggtg caaaaattac tcagctgcgt gtaccgcgcc
    13861 ggacaacgtt ttgccgccgg ttacatcacc aaccttttgc gcggtaaaag cgacgattgg
    13921 atacgcggca accggcacga acaactgtcc acattcggca tcggtgcaga attgtccgac
    13981 aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg gctacctcac cgtcaacatt
    14041 gcccgatatc aggcattgca actgaccgaa gccgccaaaa aagtcctcaa aggcaaaacc
    14101 ggagtgatgc tgcgtccgct caaacgcaac aagcccgccg tccgcaccct caaagacaac
    14161 tggctgcgta ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa
    14221 caggcagaag ccgaaggcat ccccgcctat atgattttcg gcgacaaaac cctgcgcgac
    14281 cttgtcgaaa aaatgccgca aaacctcaac gggctgcacg acatctacgg cttgggcgaa
    14341 gccaaaaccg aacgtttcgg acacggcata ctcgaagtct gccgcaacgc cgccggcttc
    14401 agtcgcgacg cggtcatccg tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc
    14461 gaggcctggc ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc
    14521 gacgaaagcc ttgccgatat gcttgccgcc acgcccgaaa ccgaaaccga cctcgaaggc
    14581 gtacacggct tgggcagcgt acgcgccgcc aaatacggac gggacatcct cgccgtctgc
    14641 cgtccgtttt cagacggcat cgatgaagcc gccaaacgca aacgccgcct gatgcgcgcc
    14701 ctgatccaat ggtgcggcga aacggcaaaa cacgaacagt ccgaacccta ccgcattctc
    14761 agcaaagccg ccctgcgcgc cattgccgcc aaacagccga agggtttggc ggagcttgcc
    14821 gccgtatacg gcgtgggcga agaaaaaacc gcacgttacg gtgcggcggt gttggcggtg
    14881 ttgggacaaa atgccgtctg aaaaccgtta ccaagtttca gacggcattg cctctattta
    14941 aaaattcctg
//
LOCUS       DAWFKN010000048        14839 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFKN010000048 DAWFKN010000000
VERSION     DAWFKN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.048"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(148..843)
                     /locus_tag="WGR46_001376"
     CDS             complement(148..843)
                     /locus_tag="WGR46_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0856303.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1014..1673)
                     /locus_tag="WGR46_001377"
     CDS             complement(1014..1673)
                     /locus_tag="WGR46_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0856304.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1871..3938)
                     /locus_tag="WGR46_001378"
                     /pseudo
     CDS             complement(1871..3938)
                     /locus_tag="WGR46_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4124..4729)
                     /locus_tag="WGR46_001379"
     CDS             complement(4124..4729)
                     /locus_tag="WGR46_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0856305.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLATVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4778..5176)
                     /locus_tag="WGR46_001380"
     CDS             complement(4778..5176)
                     /locus_tag="WGR46_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0856306.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5284..6195
                     /locus_tag="WGR46_001381"
     CDS             5284..6195
                     /locus_tag="WGR46_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0856307.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6185..7486
                     /locus_tag="WGR46_001382"
     CDS             6185..7486
                     /locus_tag="WGR46_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0856308.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7549..7845
                     /locus_tag="WGR46_001383"
     CDS             7549..7845
                     /locus_tag="WGR46_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856309.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7879..10029
                     /gene="dinG"
                     /locus_tag="WGR46_001384"
     CDS             7879..10029
                     /gene="dinG"
                     /locus_tag="WGR46_001384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0856310.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10065..10679
                     /locus_tag="WGR46_001385"
     CDS             10065..10679
                     /locus_tag="WGR46_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0856311.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10774..12642)
                     /locus_tag="WGR46_001386"
     CDS             complement(10774..12642)
                     /locus_tag="WGR46_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0856312.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12723..14093)
                     /gene="purB"
                     /locus_tag="WGR46_001387"
     CDS             complement(12723..14093)
                     /gene="purB"
                     /locus_tag="WGR46_001387"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0856313.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14165..14797)
                     /locus_tag="WGR46_001388"
     CDS             complement(14165..14797)
                     /locus_tag="WGR46_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0856314.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3542 a         4105 c         3822 g         3370 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccgct atatattctg atttaaaacc ataaggcttt
       61 aagcaatcat ctcttctata aagcctaaat acaaaaggcc gtctgaaatc ctattttcag
      121 atggccttta ctcattcaat cctcaactta ttgcggcttt ttctgctctt cgcgtacttt
      181 atccaccaac tggtcaatcg tggtcatacc ggactgccag tctttaaatt caacttggta
      241 tttgccgccg acaatcacag tcggtgtgcc gctgatttgg aatttattgg tcaactcttc
      301 catttgagcc gcacgcgctt ggctttcagg agcctcaaat gcagccaata cttttttgcc
      361 gtcaaacgct gtttgctcgg acagccattt tttcagggta tcggtatcgg ccagattgat
      421 tttttgatta accatcgcat cgaaaatatg gctgttggct ttatctgatt caccggccat
      481 ttccactgcg gccgccaaac gtgccaaagg tttcatttca tcaccccaca cgacatgctc
      541 ccggcgcata taggtatcgt ctttaaacgt tttgatgtgc tcgctcaaga ccggctcaag
      601 atgggcgcaa tgcgggcaga agtagccgaa aaattccaat acttcgattt taccggcctg
      661 ctgttgcgga ataggcgtag acaatacagt gtagttcaca ccttcgttca actcagcagg
      721 ggctgccgga gcagatgagc tgctttgggc gctgtctgcc ggaacactgg tttcagcctg
      781 tttgctacaa gcggccaatg ccaacagggt caatgaagtc aaagctaagg ttttcagttt
      841 cataggtatc tcttgtgtgt cagattaatg tgcggatttt atggcatttt attgaaggcg
      901 tgttcgattt gagtgaaaaa atgtgtttag ttttatttca gcctttataa aggccgtctg
      961 aaatatggct tagataatta gccttatttt cagacggcct ttgttctagt aatttacggt
     1021 ttcggacatt ttgtcttcaa tccgtcgatg tgttccatta agtcaggtgg gacatgattc
     1081 cacataggat caagaataaa atcgatacct tcccgtctgg ctaatttggc tgctgggaca
     1141 aaatcggaat caccagaaaa caagatgatt ttatcggctt gtttttttaa ggtaatagat
     1201 gaaatatcca agcctatacg catatctaca cctttttgtt taacatcgag cacgacatcg
     1261 ttttccgtaa tttgagttga cgggtttttc agaagatttt tcatgacttg gggttttaat
     1321 atccatgctg tattgtccga cagcttgcct aaacgtaacg ctaatttacg cttcttcttc
     1381 aattcggtat gcagattgca acgaaatatg gcttccggag tctttgaaaa atctatggca
     1441 cgatgagata cgggattgtg cattttcttt tcaaacggca cgcagtcgta aaagaaaatt
     1501 ctatataaat catccatttg atctgtgccg ttgattttat ctttcagatg cagcatcgaa
     1561 tatcggaaag ccaaatcagc aattcttttt gcatcgtgtg gattttgaga caggcctaat
     1621 gaccgtaccc gcttaataaa aaatgcaccg tcaatcaaaa tggcggtttt catattgctt
     1681 cccctatatt tgtcaaagat ataaaaaagc ccttgggatc ggcatacccg ctatgattta
     1741 atgggtaatg cgtactgcca agggcactat ttctttttgg attatactga tgtagataaa
     1801 gcctgtcaaa aagttcgtat gctgaattta cggattatca ggattttcag acggcctttg
     1861 cctgatttca tcagaaacgg taatttaccc gggcagtata gccgcgcgga ttacccggca
     1921 tagagtccga acgccaatat ttttgattga acagattggc tgcggcaaag gtaacgttaa
     1981 catttttatg gttccagcca agcatggcat caactcgggc aaagcctgga agcgtagtca
     2041 cttctttatt tcttgagtcg taaccgtagc gtttgcctgt accggttacg ccgatttcgc
     2101 cgtagaggtt ttcggtcggg gtataacgga aaaacaggtt gccggtaacg ttgctggtgt
     2161 tattcaaatg gatgcccact cggtcgggat tttctttgtc ttcaacgact ttcgcctgca
     2221 tcacgcccaa cgaaccgcgc agatagagtt tttttgggga tgatttgccc gatggcggac
     2281 aattccacgc cgcgcgaacg gtgtttgccg ctaaccgcat aaatataagg gttgtttttt
     2341 ggatcggggc ggtagcggat attgaagcgt tcgatttggt aggcagacaa cgtagtgctg
     2401 aggcggtcgt ccagccaact gcttttcacg ccggtttcgt attggcgggt gtactcgggg
     2461 tcggcgttga acacggcgga agacaacgta tcgatgctca aatagccgcc gcgtccgcca
     2521 taaggcgcga agcctttgtt atacgaggcg taaagtgtgt ggacgggatt gatgttccac
     2581 actgcgccga tgttggggct gaacgagtgt ccgctgtatt ggcggctgct gccggtgagt
     2641 ttgttttcgg aattaaaggt gtatttgtcg taacggccgc cgaggacgaa tttcaaatcg
     2701 ggcgtggcgg agaagatgtt ttgcacaaag atgccgtagg agtcggcttt gtggcggttt
     2761 tgggtcagaa taggctgcaa tctgcccgaa gccggccagc ttgcgcggtc gtaggggttg
     2821 atggaggcgg aaaaggcgct gctgaaaccc aatgtcgggt tgcggtgttc gcggctgtaa
     2881 tccatgccta cggtcaggtg gttttcaaaa cggccgatgg tgtagtcgcc gttgagcgtt
     2941 aagttggacg acagggtttt gttgtcggtc tgctgccagg cgtagttacg tttgattaag
     3001 ttgccatttt cgctgcctgc atagaaatga tcaaaatcct gcgccgccgt gcggtgggcg
     3061 agctgccatt gggcacgcca tttgtcgttg aaggcgtatt caaggtcgga acgccaaact
     3121 tgcagcttgt ctttgacaaa atcgttccgg tgggcgaacc ccatgcggta aggcagtccg
     3181 aagcggtcgt acacggactt ggtcggactg cggtcgggcg tgcgctccac attgtcgtag
     3241 gtgtattgcc ccgtccactt caagccgttg tcgagtttga cggtaatgct gggcgaaacc
     3301 atgacatttt tgctgtctat gccgctgcgg aacgaattgg cgcgcccgac ttcgccggtg
     3361 agacggatgg cgacgttttt gttcagcact tcgttaatgt ccatattcag gctgcggttt
     3421 gcccacgaac cgtaaaccgc accgatgttg cggctttgtt tgaagttggc gtatttgctg
     3481 accatgttga tgacgccgcc gccgttggtg cggccgtaaa gcacggaaga cgggcctttc
     3541 aggatttcca cgcgctcgat gttggcagta ctgcggcgca cttgtccgct ttcgcgcacg
     3601 ccgtcgcggt aaatatcgga tgcgtcggct tgaaaaccgc gcaggaaaat gctttcaccg
     3661 cgcatatcgt aggcagcgtc gatgccgaca ttgccttcga ggatggaact caaatcgttc
     3721 gtgccgtaat ttttgttttt ctggatattg cgggtaacgg cggcttcgtc gtagttgatg
     3781 tagcctttga gtacgctggt gtcggactgt ccgaccacgg aaacggtagg tagcgtggcg
     3841 gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcga cagggaagga agcaataatc
     3901 agcgtgggta ataaagctaa atgaaatgat attttcattt ttatattcaa tttaacaaaa
     3961 caaccgaatt atattgcctc acggaggaaa tgagaataat ttcttttaac tatattgaac
     4021 atgatatttg taaacaaagg tctcagaatg cggaaaactc gccgcctgat actgaaaaat
     4081 gccgtctgaa cagggttcag acggcatttt tttgaccgcg aaattatgcg ccgaacactt
     4141 tcaaacgttc tgcaactggt tcaaaggtct tttcacctgc cgccccttca ataccgccga
     4201 taaccatttg tgcgcgcaac agccagtttt cgggaatatt ccacgctttg gcaatcgccg
     4261 catcgggcaa ggggttgtaa tgttgcaggt ttgcacctac gccgaccgtg gcaagtgtcg
     4321 tccagacggc atactgtacc atcgcgttcg cctggtccgc ccaaacggga aagttggcgg
     4381 cataagcagg gaactgctcc tgcaaacctt tgacgacatt ttgatcttca taaaacaaaa
     4441 tggttgccgc acccgcctta aacaggttca atttttgcgc ggtcggttca aaactgtcgg
     4501 caggcacggc ggcacgcagc gcgtcttcga caaattgcca caccttatca tgctcttcgc
     4561 caaacagcac gaccacgcgg gcagattggg aattgaacga agaaggtgtg tgcaaaacgg
     4621 cgtgttcgac gatttggaca acttcatctt tgccgacggg cagattttta tttaacgaat
     4681 aaatggaacg gcggctttcg gcagcctgtt gcagagattg acgggtcatt tcttttcctc
     4741 aaaaacattc aacaaaatac actttaggca gtccgattca ggctttacgc ttgaaaaaca
     4801 gcctgtccag cgaccatgct ccgccgcccg ccgccgcgat atagaggaat acgaagcaga
     4861 acagcactgc ggactcgccg ccgttggcaa tcgggaacaa agcatttccg gaagcgtgcg
     4921 ccataaaata ggcaaccgcc atctggccgg acaaaacaaa cgcggcaggg cgcgcaaaca
     4981 ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat accggcaagc agcaacagcc
     5041 cgccgggcga accgctgccc atttcaatgg ggaaggcgaa gattttcgac gtaccgtgca
     5101 acaaaaacag gtaagcggtt acgatacgca aaacagaaag caaaaccggt tggatacggt
     5161 tgcagcaatc ggacataaaa gctcctttga tatttgtctt catttcaggc ggtagagtat
     5221 agatgtttcc ccttttcaaa tatatactgc ttttattgat acaacttttc cacacaagaa
     5281 aatatggaca ccctgttcag cctcaaggtt ttccgccaag tcgtccaaag cggcggcttc
     5341 acccgcgccg ccgacgcgct cggcatctcc accgcaatgg caagcaaaca cgtcagccac
     5401 ttggaaaaca ccgtccaagc caaactcctg caccgcaaca gccgcaacct cagcctgacc
     5461 gaagccgggg aagaatacta ccggcaatgc agttacgcgc tcgacacgct cgacgatgcc
     5521 gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc tgctgcgcgt aacgatgcca
     5581 ctgtggtttg ccggcagcca gatatgcaac tggctggcgg aataccgcga acgttatccc
     5641 gaagtgacat tagaactgat tttggacaac cgccacgtcg atttgattgc cgaaggcgtg
     5701 gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcgc tgattgcgcg cccgctggcg
     5761 gaaatcgaat ttgccctgct cgcctcgccc gatttcctga ggcgcaacgg cgtgccggaa
     5821 acgccggaag aagtggcggg gctgcccgcc gtcctgccga cctacaccaa ccagcagaaa
     5881 ctcgacctca cccgcaaatc ggacggcaaa aaataccggc ttgaactgac ccccgtcatc
     5941 cgtaccgaca acacgctgat gatgcgcgaa atgattaagg cgggcgcgtg catcggttat
     6001 cagccgcttt gggcggcgga acacgatttg cgctgcggca cgctggtgag gctgctgccc
     6061 ggatacgccg tcccgaccga ccggctgaat gccgtttatg cagacagggc attcttaagc
     6121 gcgaaagtcc gcagcttcat cgattttctg aacgaaaaaa tcgccagcag gaaaggctgc
     6181 cgaaatgccg tctgaaaccg cccgccccct ttatgcggac acgtgcgccg cacacacgct
     6241 cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc gccttatgca ccgccgttgc
     6301 cgaaggtttg cccgttatcg gtatttggat tgacgatgcc gaaacagaca accctcgccg
     6361 cgccgcgttc taccgccaat ccgccgccga actcgcccaa gggcttgcag ggcgcggcat
     6421 cccgctctac acggcggcat cgcctgccga gctcgtccgg ctcgccgtcc gcctcaatat
     6481 ccgcgccgtc atcgccgacg aatcccatac ttttgccgac aaactcgccg acaacgccct
     6541 ttggcatgaa ttggacaaac acggcatcgc gttaaccttc gtcaatgacc gcgccgtttt
     6601 cggcaaaacc gacctgatac ccgacggcgg cacggcacac accgatttcg accgctaccg
     6661 cgaagtatgg ctcgaccgct tttccaagca gccccccgcc ggtccggacc tattcgcggc
     6721 ataccgccaa cccttccccg aaaacctttc cgccccgccg cctgccgccc tttcagacgg
     6781 catcttcctg ccgcaaaaca gcggcgaaac ggcggcttgg cggcagtggc ggcggtttct
     6841 cgaacaggcg gattcctact ccgttttaaa ggatttcccc tcgcgcaaac acacttcgct
     6901 gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc ctgctcgcgc gggaaagcct
     6961 cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc cgcgattttt tccttcaact
     7021 tgccttgcag cacacggatg acgacccttc agacggcaat cctgaacaca ccctgcgcct
     7081 gacgctttgg cagcagggcc ggaccggcat tccgattatc gatgccgcga tgcgctgttt
     7141 gcacaaaacc ggcagcctcc accccgccct gagacgcttg agcgcggatt ttttctgcca
     7201 cgttttaaac ctcccccgcc gcgaaggcga gatatggttt gcccgacagc tgaccgattt
     7261 cgatgcagca atcaaccaag gcaactggcg gcttgccgcc tcacggcaca cctgccccga
     7321 cattgccgcc gccgcacaca agaccgaccc cgacggcacc tttgtcagac ggcacattcc
     7381 cgagcttgcc cacctgtccg ccgacaccgt ccacacgcct tggcggtttg cctgttcggt
     7441 cgatacccac ggctatcccg cccatcctgt cgccggtatt ccctaagcgg tacggcaacc
     7501 taaaacaaat gccgtctgaa cttccgtttc agacggcatt gcgtccgaat gactcaatat
     7561 cttccgtttt tttcactttc aatcatcagg tatgcccaaa tcaccgccac ccaagccgcc
     7621 accgttccca tcgtcggcaa aaaatgctgc caatccgtca tcggcggtac agggcaacgg
     7681 ccgcattcga tttccgcgcc gcccaacaac cggtacagca gcatatccgc aatacaaagc
     7741 aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc acgtccgcac gatttacctc
     7801 cccccattcc gacaacgata ttgtaccgct tccgtgcaat gttaaaataa ccccacttct
     7861 accgccgtac aaaacgccat gctcaccgat ttagaaaaaa acgccatccg cgaccattac
     7921 caaaacatcg gcaaaaacct gcccggtttc cgtccgcgtg cttcgcagcg ggaaatgatt
     7981 gcggcggttg ccaacgcttt ttcgcggacg ttgacgcgtg aagaaggcga agaagcaccc
     8041 aaacgtgagg gcgagagcat tgccgtgatc gaaggcccga ccggcgtggg caaatcgttg
     8101 gcctaccttt tggccggcgg catcatggcg caaacgcgcg gcaaacggct gattgtcagc
     8161 agcgcgacgg tggctttgca ggagcagttg gttgaccgag atttgccgtt tctggtcgaa
     8221 aaaagcggtt tggagctgac ctttgcgctt gccaaagggc gcggccgtta tctttgtccg
     8281 tacaaactct atcaactgac gcaaaacaac gcccagcaaa acctgcttgg ttttgaagcg
     8341 ccggaagtgt tgtgggacag caaacccaag cccgaagaat tgaacctgct gcgcgacatc
     8401 gccgacgaat tttccgcccg acggttcaac ggcgaccgcg acacctggcc ggaaaaaatc
     8461 gatgacgcga tttggctcaa agttaccaac gaccgccacg gctgcctgaa agccgcctgt
     8521 cccaaccgcg ccgaatgccc gttttacctg gcacgcgata tgttggaaac cgtcgatgtc
     8581 gtcgttgcca accacgatct tctgcttgcc gacatcagta tgggcggcgg cgtgattctg
     8641 cctgcgcccg aaaacagttt ctattgcata gacgaagcgc accacctgcc caaaaaagcc
     8701 ctcagccgtt ttgccgccga acattcatgg aatattgccg tttggacgct ggaaaaactg
     8761 ccgcaactga ccggcaagat tgccgcgctg accgataaag ccgaacttgc caaccttgcc
     8821 gacgaagccg cctcatcctt actcgacagc ctgcacgaat ggcagttcca tttggcggaa
     8881 gagccgtctt tgagcttggg gccgtctgaa aacgacaggc gtaccaacag cgaaccgact
     8941 tggctgtggg aagacggcaa aatccccgaa ggcctcgaaa ccaccgtttc caacaccgcc
     9001 attgccgcac gcagcctgct caaacacgtt atcgggctga acgatgcgct ttccgccgcg
     9061 cgccgcgaaa aagaacaaga cagcgcgctc atcgaccgcc tgaccggcga gttcggcctt
     9121 tttatctccc gtatcgaaca aattagtgcg gtttgggatt tgctctccac cgttcccatt
     9181 gaaggcgaag agccgttggc gaaatggata acccgccgtg ccgacgataa aaacgactat
     9241 attttcaacg ccagccccat cagcagcgcg tcccaccttg ccaacagcct gtggcggcgg
     9301 gcggcaggcg cagtgttgac ctccgccacc ctgcaatcct tgggcaactt taacctgatg
     9361 ctgcgccaaa ccgggctgca atggctgcct gaaaccacca ccctcgccct caaaagcccc
     9421 tttgactttg aaaaacaggg tgaactctac atccctccca tatacgccag ccccaaagac
     9481 cccgaagccc acaccgccgc catcatcgag tggctgccca agctcatttc gcccggcgaa
     9541 gccatcggca cgctcgtcct gttttcctcg cgcaaacaaa tgcaggatgt cgccttacaa
     9601 ctgcctgacg actatctgcc gctcttgctc gtacaaggcg aattgcccaa agccgttctc
     9661 ctgcaaaaac accaccgggc catagaagaa ggcaaagcca gcatcatctt cggactcgac
     9721 agctttgccg aaggactcga cctgcccggc accgcctgcg tgcaagtcat catcgccaaa
     9781 cttcccttcg ccatgcccga caaccccatc gaaaaaaccc aaaaccgctg gatagaacag
     9841 cgcggcggca accctttcat cgaaatcacc gtccccgaag ccggcatcaa actcatccag
     9901 gccgtcggcc gcctcatccg caccgaacaa gactacggcc gcgtaaccat cctcgacaac
     9961 cgcgtcaaaa cacagcggta cggccaacaa ttattagccg gcctgccacc gtttaaaagg
    10021 atagggtaaa cctaccgccc tgcccgcaac agaaaggaaa aatcatgaac ttcacccgac
    10081 tgctcaacca agtcttaagc acggttcaaa aaaaaggcaa caccttctcc gacagcccgc
    10141 tcaattcatt cggcggaggc gcgctggttg ccggtgtcgc ctccatgctg ctgaacggta
    10201 aaaaccgcaa aaccatcacc aaaatcggtt cgaccgccgc tttgggctac ctcgcctacc
    10261 ggggctatca gatgtggcag caaaacaaag ggcgggcaac cgtaacacaa agcgatttcc
    10321 aacctgccgg agaaaccgaa gaaacataca gccgcaccgt attgcgcacc atgatagccg
    10381 ccgccgcttc agacggcatg atagacgaag ccgaacgccg gactatcgaa caggaaagcg
    10441 gcacagaccc cgaaactgct gcatggctcg ccgccgaata ccgcctgccc gcaagcatcg
    10501 aagacatcgc cgccgccgtc ggcaacgatg aggcgttggc ggcggaggcc tatctggcgg
    10561 caagactggt atgtgccgat ttgtcgcgga aagaaaccgt cttcctcgcc cgcctgtcgc
    10621 aggctttgga actggatgac aatctggtgg aaaacttgga aaggcaattg gggttttgat
    10681 tcaaaattcc ctcccgcagg ggaaggaaaa taaaaaccgc cgccgccccg aaaggcaaac
    10741 ggcggcacaa acgatatcgg acggcaaggc aggctactgc aatgcgccgt cgcgagaaga
    10801 tttaagcagg cgttcgtgtt tgtgaaattc ccgccccata taataagaac cgaaataggt
    10861 caggtggtct tggtcgccgt aaagatagcg gccgtatatt tcgaccgtgt ttttgggcag
    10921 gtatttttgt gcgtccaccc aatgcacatt gggaatatct ttaatcaaat caaagaccgc
    10981 ctgattgctc ttgccgatgt cgcccatagc ctgaatgggg cggagatatt ggtttgcggc
    11041 aaatcttttc aatttttcct ccctcagggg cgaacggctg attgatgtgt tgtttgcaaa
    11101 aacatagacg ggtttgacgg cggctatcct tttgacggtt tccctgaatc gggctgggaa
    11161 cccgggtatt aggaaggatt gcgcttcaaa tctcggcacg ggctggccgc ccatcctcaa
    11221 atcatagaat tgggcaatga aaacggcttc ggctttttca acttcatccc ggtattttcg
    11281 acataacggg ttgtctgcca gcttctcatc tacccaaacc aaacactccg aatcgaggga
    11341 caggattttg gctttccacc cttcccggct gccgacataa tccagaaacc cccgcaggtg
    11401 tccggcgtgc gagtcgccga gggtcaggac ggtttccgga aaatgatttt ccgcagcaag
    11461 gggcgcgccg ggcaacgggc ggaggtgttc ctgtttcaat atcccccttg cgtacaggtt
    11521 gtaaccgaca agtatcaggg acggggcgag atagaggcag aaaaatgcct ttttgaaggt
    11581 catcttccgt tttctaagcg gctgttcaat caaataataa ctcaacaggg aaaatccggc
    11641 cgtcaacgcg gcaaccgccg atacggcagg cagtccgagc tgtttgtcgc ctgtaatgta
    11701 atgggcgaaa gcaataaaaa tccaatggta caggtatagg gaataagaga ttttgccgac
    11761 aaatacgatg gggcttgccg acaggatgcg ggtcggaagt gtcccgtatt gcatactccg
    11821 gataagcagt gccgtcagca ggcagggaag gagcagggtc attcccggga taaacggatt
    11881 gtgtttgtca atcacgaaca ggcaggcaag caatgcgccg aagcagagtg atgaaagcaa
    11941 ctgccgtttt ccatttgctg tttgccgtct gccgttttgc gtttgcccgt aaaccgccag
    12001 cagcgaacct gccaacagct cgggaaacct cagtgtcgaa aggtaataag tattgggttg
    12061 gttgagaata tcggtataaa acccgcttgg caaaaacgat gtggcagtca aaatcagaaa
    12121 taggatgatg ctgatgttac gcagcacccg tagcgatttt gtttttttgc agcaaaatat
    12181 cagcaaaaga ggatacagga ggtaatactg ttcctctact gccaaagacc agatatgcag
    12241 tacggggttc tcgtcggcac tcaaatcgaa atacccctgc tgaaacccca gataaatatt
    12301 ggacaagaaa accgcagaaa gctccacggt tttccgcatt tggttgaaat cttcgtaaag
    12361 gaagatttga gaggcaatca ccgaagccag cgacacggcc gcaataaaag caggataaat
    12421 ccgcttaatc ctgcgggtat aaaaatcccg gaaagaaaaa gaaccgttct gtatttcaga
    12481 aagaatgatg ccggtaatga ggaatcctga gatgacaaag aaaatgtcca cccccaggaa
    12541 tcctccgggc agccagcggt tattcaggtg gaaaatcatg acggatagca cggcgacggc
    12601 ccgcaatccg tcaatttccg gtctgtatcg gacagcttgc ataaatatcg cccctgtatg
    12661 tcccgttatc ttaaaaatgc cgtctgaacg cgcgttcaga cggcatcggt ttcagtaaac
    12721 gctcaaatcc gtttcgccaa cgcttcagcc ttgcccacat acagcgcggg ggtcagctca
    12781 agcaatttgg ctttggcttc ggcggggatt tccagcaatc cgataaagcc tttcagcact
    12841 tcgggcgtga tgccgccttt gccgcgcgtc aggtctttca gcttctcgta aggattggcc
    12901 acgccgtaac ggcgcattac ggtttgaatc ggctcggcga gcagctccca agtggcatcc
    12961 aaatcggcgg caagcgcggc ggggttaggt tcgagcttgt tcagaccgcg caggtgggcg
    13021 gcgaaaccca acacggcata gcccacgccc acgcccatat tgcgtaagac ggtgctgtcg
    13081 gtcaggtcgc gctgccaacg ggaaatcggc agtttttccg ccaaaaaacc caatacggcg
    13141 tttgccatac caaggttgcc ctcggagttt tcaaagtcga tggggttgac tttgtgcggc
    13201 atggtggaag aaccaacttc gcccgctttg actttttgtt tgaagtaccc caaagaaatg
    13261 tagccccaaa cgtcgcggtt aaagtcgatc aggatggtgt tgatgcggct gagggtttgg
    13321 aagaattcag ccatgtagtc gtgcggctcg atttggatgg tgtaggggtt gaaggtcagg
    13381 ccaaggctga tttcgacgaa gttgcggcag tgggtttccc aatcgacgtc gggataggcg
    13441 accatgtggg cgttgtagtt gccgaccgcg ccgttgattt tgccgaggaa ctcttgcgcc
    13501 tgaaggcttt taaactggcg ttgcaggcgg tacacgacat tggcggtttc tttgcccaaa
    13561 gtggtcggcg tggcgggctg gccgtgggtg cggctcatca tcgggacggc ggcaaggtcg
    13621 tgcgccatag cggtcagttt ttcgatgatt tctgccagtt tgggcagcaa aacagcctca
    13681 cgcgcttctt gcagcattaa agcgtgggac aggttgttga tgtcttcgct ggtgcaggcg
    13741 aagtggatga actcactcac ggcggcgact tccggcactt cggcaaaacg ttttttcagc
    13801 caatattcaa tggctttgac atcatgattg gtcgtggctt cgatggcttt gacggcggcc
    13861 gcgtcttcca atgaaaagtt ttcaatcacc gtgtcgattt cggcaagcgt ttcggcactg
    13921 aagggcggca cttcggcaat cttcggctcg gcggcgaggg ctttgagcca gtttaattcg
    13981 actttgacgc gcgctttcat caggccgtac tctgagaaaa tcgggcgcaa tgcttcaacg
    14041 gattgggcat aacggccgtc taaaggggaa agcgaggcga tggggttgat catttcggca
    14101 tcctgttcgg gaaagacatc aaaaattgtt aaattgtttg caattataca tcactctcag
    14161 gacgctatgc cgtctgaagc ccgtgttccg acagatacgc aacgatttcg tcttcatcgt
    14221 ccgatatggc gaacaaagag gctgcccctt cggaaatcag accgcgcgcc aaaagctgcg
    14281 cgtttatcca ctccgccaag cccgaccaaa acgcctttcc gaccaaaaca atcggacgcg
    14341 gcggcacttt gcccgtctgc accaaggtca ggatttcaaa caattcgtcc agcgtcccga
    14401 agccgcccgg catcacgaca tatgcttggg aatagcggaa aaacaccgcc ttgcgttcgg
    14461 caaaacggga aaaccgcaag gcgatgtcct gatacggatt cggtttctgc tcgtgcggca
    14521 aaacgatgtt cagccccacc gaaaccgact tcccggcaaa cgcgcccttg tttgccgcct
    14581 ccataatccc cggcccgccg cccgaaatga cggcaatgcc cgaatccgac agccgccgcg
    14641 ccagacggca ggagaacgca taatccgcat gattctgcgg cgtgcgcgcg ctgccgaaga
    14701 tactgactgc cggaaacacg cccgccaatg cttcgtctgc ctgcctgcgt tcggcatcat
    14761 aacgtgcctg ctccggcaca cggtttgtat tctccattcc atcctccgtt caaaaacagc
    14821 gattgtacac cgtcaaaaa
//
LOCUS       DAWFKN010000049        14822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFKN010000049 DAWFKN010000000
VERSION     DAWFKN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.049"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGR46_001389"
     CDS             complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGR46_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0856315.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1720..3243)
                     /locus_tag="WGR46_001390"
     CDS             complement(1720..3243)
                     /locus_tag="WGR46_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0856316.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            complement(3550..4356)
                     /locus_tag="WGR46_001391"
     CDS             complement(3550..4356)
                     /locus_tag="WGR46_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0856317.1"
                     /translation="MNMKKWIAAALVCSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4408..4659)
                     /locus_tag="WGR46_001392"
     CDS             complement(4408..4659)
                     /locus_tag="WGR46_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856318.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4759..5520
                     /locus_tag="WGR46_001393"
     CDS             4759..5520
                     /locus_tag="WGR46_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856319.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDIRKILNDIKYELKYKR"
     gene            5818..6987
                     /gene="metZ"
                     /locus_tag="WGR46_001394"
     CDS             5818..6987
                     /gene="metZ"
                     /locus_tag="WGR46_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0856320.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALESAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7073..7279
                     /locus_tag="WGR46_001395"
     CDS             7073..7279
                     /locus_tag="WGR46_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0856321.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7378..7490)
                     /locus_tag="WGR46_001396"
                     /pseudo
     CDS             complement(7378..7490)
                     /locus_tag="WGR46_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8412..9164)
                     /locus_tag="WGR46_001397"
     CDS             complement(8412..9164)
                     /locus_tag="WGR46_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0856322.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9268..9735)
                     /locus_tag="WGR46_001398"
     CDS             complement(9268..9735)
                     /locus_tag="WGR46_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0856323.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9787..11304)
                     /locus_tag="WGR46_001399"
     CDS             complement(9787..11304)
                     /locus_tag="WGR46_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ0856324.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGR46_001400"
     CDS             complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGR46_001400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0856325.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     AIAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEAEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            13924..14607
                     /locus_tag="WGR46_001401"
     CDS             13924..14607
                     /locus_tag="WGR46_001401"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0856326.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
BASE COUNT         3118 a         3682 c         4381 g         3641 t
ORIGIN      
        1 tgaatattgc cgctgtccaa tggtttggat tcccgcctgt gcgggaatga cgatttggaa
       61 attacccgaa acccaaaaac aactgaaacc gaacagaccg gattcccgcc tgcgcgggaa
      121 taacggtggg ggacgatgcc gtctgaagcc cacctaccat tattccgctt ccataccgct
      181 tggcggcaat gtgtccggaa tggggcggtt tatccgaaaa agcccatttt gacttggatg
      241 agtttttcca gttcgttcag gatgcgccgg cgcgagacga aaaagatgat gtggtcgccg
      301 tcttggatga cggtttcggt atggtgtccc ataatggttt cgccggttcc ggcgcggacg
      361 acggcggcaa tgtggcagcc ttcgggccat ttgatgccgc tgatgcgcct gccgatgatg
      421 gcggaagttt ttttgtcgcc gtgtgcgacg acttcgatgg cttccgccgt gccgcgccgg
      481 atggggtgga cggcaacgat gtcgccgcgc cggatgtggg cgagtatcga gccgatggtg
      541 atgaggtggg gggagacgac gatgtcgatt ttgttgcctt cgagcaaatc gacgtagctt
      601 gagcggttga cgatgccgat gacgcgcttc gcgccgaggt ttttcgccaa aagggcggac
      661 ataatgttgc tttcgtcgtc gttggtcagg gcgcagaata cgtcgatttc gtcgatgtat
      721 tcgttgtcga gcagggtttc gtcggttgcc gaaccttgca ggacgagggt gttgtcgagg
      781 ttttcggcta tccattcggc acggcgcggc cggcattcga tgattttgac gttgtatgcg
      841 tgttcgagct gcttggcgag acggtagccg atgttgccgc cgccggcaat catgatgcgg
      901 cgggtgctgg tttctttggg gcgcaattcg ggtatgaccg cgccgatgtt ttcggcggcg
      961 gcggcaaata ggatttcgtc gccttcgatg atgacggttt gcggcgcggg gacgatgagg
     1021 cggttgttgc ggtaaacggc gcagatttgg cagtcggccc cgtcgggcaa atcttgggcg
     1081 atgtcggcaa tgctgcgtcc gacaagcagt ccgccgcgcc gcgcctgtat gatgaccatc
     1141 cgcacgcggt cgtctgcaaa acgtaaaacc tgcaatgcgc ccgggcagtc tatcaggccg
     1201 gcaagctgtt cggtaacgag ctgttcgggg ctgatggttt cggttatgcc gaatatggaa
     1261 aggctgccgt tttcgttgtt ttcgagcttg gggctgaggt attcgaggta ttcgctggaa
     1321 cggacgcgcg cgatgcggcc ggggatgttg aacaggtcgg cggcaacttt gcaggcgacg
     1381 atgttggttt cgtcgctgcg ggagagcgcg agcagcaagt cggcatcttc cgcgccggcg
     1441 cgttctaatg tgaaggggga tgcgccgttg ccgaaaacgg tttggacatc gaggcggctg
     1501 cctgtttcct gcaatgcttt ttcgtcgatg tcgataacgg ttacgtcgtt gttgggtatg
     1561 gcggcaaggt tttgtgcgac ggtagaacct acctgtccgt tgcctaaaat gaggattttc
     1621 acggtgtggg tcgccgagtg atgaaatggt ggattgtacc gcaaccaggg gcggaaatgc
     1681 cgtctgaaag cagattttgt gttcagacgg catgacgcct caagatttga cggggatgat
     1741 gccgattttc gcctgccatt ggggcggggc ggtggcgtgg atggattcgc ctttgctgtc
     1801 tacggcgacg gttacgggca tgtctttgac ttcaaattcg taaatggctt ccatgcccaa
     1861 ttcggggaac gccaagactt tggaagattt gatggctttt gccacgagat acgccgcgcc
     1921 gccgactgcc atgaggtaca cggctttgtt gtcggcgatg gcttcgcagg tggccacgcc
     1981 gcgctcggat ttgccgatca tgcccaagag gtcggtttgt tcgagcattt ggcgggtgaa
     2041 tttgtccatg cgggtggctg tggtcggacc tgccggaccg acgacttcat cgccgaccgg
     2101 atcgacgggg ccgacgtagt aaatcaggcg gttggtgaaa tcgacgggca attcttcgcc
     2161 tttgttgagc atatcgacga ggcgtttgtg tgcggcatcg cggccggtga ggattttgcc
     2221 gttcaacagc aatacgtcgc cggttttcca gcttgccact tcttctttgg tcagcttgtc
     2281 gacatcgacg cgtttgccgt tgtcggggct gtaagtcaaa tcgggccagt cttcgacgcg
     2341 cggcggcgtg agttcgacag ggcctgagcc gtccaattca aattcgacgt ggcgggtggc
     2401 ggcgcagttc ggaatcatgg caatcggttt ggaggcggcg tgggtcgggt aatcgaggat
     2461 tttcacgtcc aacacggtgg tcagaccgcc caagccttgc gcgccgatgc ctagcgcgtt
     2521 gactttttca aagagttcga ggcgcagggc ttcggtggtg gacaattccg cgccggacgc
     2581 ggctttttcc tgcaattctt ggatgtcgat gtggctcatc agggattctt tcgccatcaa
     2641 cacggctttt tcgggcgtac cgccgatgcc gatgcccaag atgccgggag gacaccagcc
     2701 cgcgcccatg gtcgggatgg ttttcaatac ccaatcgacg atgttgtcgg aaggattgag
     2761 catggcgagt ttggatttgt tttcagagcc gccgcctttt gccgcgcagg ttacttcgac
     2821 tttgtcgccc ggcacgatgc tcatatggat gacggcgggc gtgttgtctt tggtattttg
     2881 gcgtttgccg gcggggtcgg cgagaacgga agcgcgcagc gtattgcctt cccaagtgta
     2941 ggcgcggcgc acgccttcgt taaccatttc ttccacgctc atatccgcat cccactgcac
     3001 atccataccg actttcaaaa acacggttgc gataccggta tcttggcaga tggggcggtt
     3061 gttttccgca cacatacggc tgttgaccaa aatctgcgtc atcgcgtctt tggcggcggg
     3121 attttcttcc ttctgccacg ccttataaag cgcgtcgatg tagtctttcg gatggtagta
     3181 gctgatgaat tggaaggcat cgcagatact ttggataaag tcttcttgct tgatgacggt
     3241 catgattgtg ttccttttca aggttgtggc ggtaagtgtc tgctgctgtt ttaagcaacc
     3301 tttttgaagg ctgtttttta tcaggttgaa atagtgccag cgtttgtacg gtaaagacaa
     3361 tacataattc taatctcttg attatcaatt tgttttaata aaatactgtt ttactcggat
     3421 tttgatattt gtaatctttg cctgttgctt tgcggtaggg agaaatcagc aggcagttca
     3481 ttgtaggccg attgatgaac aatgccgtct gaaagggctt cagacggcat tgtgttcgga
     3541 cgggcgggct tattttgcgg cctgtccgtc ttcttttgca aaatatttgg cgtaaatctt
     3601 gtcgtattcg ccgctttcgc gtactttttc caacgcatcg ttcagcattt tgacggttgc
     3661 ttcgtcgcct ttgcgtaccg cgatgccgta gtgttcggtg gtgaagtcgg gcagggtaac
     3721 gaagtccatc cctttggtcg gattgttttt cacataattg gcgatgaccg cgctgtcgct
     3781 gactacggaa tccaagccgc cgttttccag ttctttgata atcaggggaa cgttttcaaa
     3841 gcgcgcgatt ttcgggttgt cgttgcccaa gagtttggat acggagaaat cgcccgtgta
     3901 gccggttacc acgccgactt tgttcatgtt tttcaaatcg tcggaagaag atattttttt
     3961 gcctttcgga acgaggacga cttgggtgat ttcaaaatac gggtcgctga agtccataga
     4021 ctgtttgcgg tcgtcggtaa tggttacgcc cgacatcaca acgtccgcat cgccgttgtt
     4081 caaggcgggg aaaaggctgt cccacggctg gtgtttgaat tcgattttaa aattgcccgc
     4141 cttcgccatc gcgttcatca aatccacatc gaaaccttcg acattgcctt tcgagtctaa
     4201 agattcaaag ggggcaaact cggcgttgga agccacgcgg tacactttgt cggggtttgc
     4261 ggcgggcgcg gcggcatctt tgccctgacc gccgcaggca gacagcgcga gtgcggaaca
     4321 gacaagggcg gcggcaatcc attttttcat attcatcaaa attcctttcg gtaatcaaaa
     4381 agcctaaaaa atcagtttaa cacaaaatta acgttctttt tcacggaaaa acgcataagt
     4441 aacgtcgtgg aacaaatcgg tatgggggct gtcctcatag ctcaattctg ccaaggcgtc
     4501 ctcaaaagca agctgaatcg actcgaccgc gatttcggcg gtttcggcgg gaagggcgcg
     4561 gattaaggct ttgaatgcgg tggacaggac gtggttctgc atacgcagca cgtcgttcgc
     4621 ctcttccaga tattccaaac gctgttcgat gctgcccatg atgtatcctc gcgaatctgt
     4681 taaaatgcgc cattatatca aaccgcgctt tgggcgcggg tttgtctgcc tgcaaagatt
     4741 aagaaccgga agccgaaaat ggatgcgaat aaagagccga attgggaaaa acgtttggaa
     4801 aacctgcgtc agacgcgccg tatgaaggca gtgccgactt acgcgccgct gtcccgccct
     4861 tctgaaaccg gaacggacgc gtctgtgccg aaactggacg atgcaagccg aaaccgtgtg
     4921 ttaaaggcgt atctgaagaa atggcaggag gagcacaatg ccgccgttgc cgattgcggg
     4981 gaagaaatcg aaaccgaccc gatgatcgtc cttcaggaaa actggctcaa cgcgcaggct
     5041 tccctgcaaa tgtcggtttc ggaaaaacac atcgaatcgc ggcggagaat ccggtttgat
     5101 gccaaaaaag acagtgccga atttgaaacc ggacagattg aaggcgggga aaacgccgat
     5161 gcggcagaat cgggtacggc ggcagacggg gttgcggaaa acggcgaagg cggtgcggaa
     5221 gaagcgttga tgcccgtcca aatcaatatc ctgaacccga aagccgtcaa ccggcgcgaa
     5281 gtgttctgcc tgtccgaaca ggagctgacc gagcggctga tcaaacgcct gcgcccgcat
     5341 ctgaccgata ccgtcaacgg catcatccgt acggcggtgc aaaagcagat ggcggttttt
     5401 acctaccagc tccagcagat gctgcacgag caggcgggtg cggtagtcga agacgtgttg
     5461 gaacacgata tccgcaaaat cctcaacgac atcaagtacg agttaaaata caaacgctga
     5521 ttgccatgcc gtctgaaagc cgtttcagac ggcattttcc tgcccgtccg acttgaaagc
     5581 ctcatggaac aggcgcaaaa tattaatata gtggattaac aaaaatcagg acaaggcgac
     5641 gaagccgaag acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
     5701 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
     5761 aaattaaaca acgggcgcac gctccgttgc cgaacggata aaaccgagga atcaccgatg
     5821 agcaaaaaac tccatccgca aacgctcgcc atacgcggcg gcaaagaaca aaccggatac
     5881 cgcgaacaca atcaggcatt gttcctgacc agcagtttca tgtgggacaa cgcccaacac
     5941 gctgccgatt tgttttcaaa aaaaatcaaa ggcttcactt atacccgtac cgccaacccg
     6001 accaccgccg cctttgaaaa acgcatcgcc gctttggaaa gtgcggaacg cgcggtcgcc
     6061 acttcgaccg gcatgtccgc gattcaggcg gcgtttttta cctttttgca ggcgggcgac
     6121 catgtgattt ccagccgcag cctgttcggc acgaccgtcg gctttatcaa taacatcgtt
     6181 accaaattcg gcatcgaagt gagccacgtg tcgccgactg atataaacga atggaaagcc
     6241 gccgtcaaag ccaacaccaa actgctgttt ttggaaacgc cgtccaatcc cttgggagaa
     6301 gtggccgact tggaagcctt ggcggaattg gcgcacggca tcggcgcgat tttggtggtg
     6361 gacaacagcc tgctgtcgcc catcggctct cagcctttga agcacggcgc agatatttcc
     6421 gtttcttccg caaccaaagc catagacgga cacggacgcg tgatgggcgg cgtgttggcg
     6481 ggttcggaag aactgatggc gcaggccgcc gtttactgca actcttgcgg gctggcgatg
     6541 tcgccgttta acgcgtggca gttgttgagc ggcgtggaaa ccctgtcgct gcgtatggaa
     6601 aaacagttcg acaacgccct gaaaatcgcg caatggctgc aagcgcagcc gcaggttcaa
     6661 gccgtgtatt acaccggatt gcccgaccat ccgcaggcgg cgcttatccg caaacagcaa
     6721 aacggcggtg gcatcgtcat cggctttgaa gtagccgacc aagaagccgc gtggaaagtt
     6781 gtggacggcg tggaactctt ttcccgaacc gccaacctcg gcgacgtgcg ctcgaccatc
     6841 acccatccgt ggacaaccac gcacggcagg atgcagcccg aagaaaaact cgccgccaac
     6901 atccgcccgg gactggtgcg cctgtcggtc ggtttggaat atgttggcga tttgattgac
     6961 gatttgaaac aggcactcgc acgctgattg cgtgtttcag acggcattgg acagcagatg
     7021 ccgtctgaaa cccttatcgc gtatggcata cacatcaaca ataggaaaaa atatggatga
     7081 agcagttttt gccgattggg cgttgaaaat ctgcctgacc ggcttgatta tttttttggg
     7141 ttttatcgtt tggaatttgg gcaaggagtc caaagcgggc aaattcggca tcgccatttt
     7201 gtttttggtt ttgggcttgg gcgtgttcgg ttttgtgttc aaagagttat tgattaagtt
     7261 tttggtattg ccgaaatagg tttgcggcct gtcaaaatgc cgtctgaaat cataaaaagt
     7321 aatctgcacc ctaaaagttt gatttaaccg ccaactgatt aaggtgcgga tttttatgac
     7381 aaaatatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt
     7441 actgtctgcg gcttcgtcgc cttgtcttga tttaaattta atccactata aaaatcatct
     7501 ttccgcagtc attcccacgg acgcgaaagt ctgtcttttt gaatcccgga taattttaca
     7561 tcgtcattcc cgcgcaggcg ggaatccaaa tcattgggta gcggcaatgt tcaaaagtcg
     7621 tctgaaaaat cagaagttct agattcccgt tttcacggga atgacggaat ttcagacggc
     7681 atcctcccgc cccgtcattc ccgcgcaggc gggaatctag tccgttcggt ttcggtcatt
     7741 tacgataaat tcctgttgct tttcatttct ggattcccac ttccgtggga atgacggagg
     7801 agcggataaa ttctcgcaat ctaaaacctt gtcatttcca taaaaaacag caacccgaaa
     7861 cagcaactta aaaacagcaa cctgaaacct cgtcattccc gcgcaggtgg gaatccagat
     7921 cattgggtgg cggcaatgtt caaaagtcgt ctgaaaaatc agaagttcta gattcccgtt
     7981 ttcacgggaa tgacggaatt tcagacggca tctgcccgcc ccgtcattcc cgcgtaggcg
     8041 ggaatctagt ccgttcggtt tcggtcattt acgataaatt cctgttgcct ttcatttcta
     8101 gattcccact tccgtgggaa tgacggcgga gcggataaat tctcgcaatc taaaacctcg
     8161 tcatttccat aaaaaacagc aacccgaaac agcaacctga aaccttgtca ttctcgcgca
     8221 ggcgggaatc tagtccgttc ggtttcggtc atttacgata aattcctgtt gcttttcatt
     8281 tctggattcc cacttccgtg ggaatgacaa actttaaggt gtgatgattt atccaaaaca
     8341 gtcgaaacgc aaaaaccggt ttctcgtttg cataagaacg gcaaattttt cggtgtcttg
     8401 ttttatgggc gttatccctt gtccgtcccg tagggcaggt aaacgtcgaa gcgggtgctt
     8461 ttgcctgtgt attggtaggc agggtcttgt ccggcaaggt gttcgccgag gcgggggcgt
     8521 ttgacgacga cgcgtttttt tgctgtttgg cgtgcggtat gcaggagtgc cgcttcatct
     8581 tgcgcttcgc cgacgagccg gtggaagtag gtcatttctt ttttaacggc ggcacttttg
     8641 cggcgttcgg gatacatggg gtcgagatag acgatgtcgg gtttgccttg tgtttggaca
     8701 agtgcgggca tttgttcggc ggcgttgccg aaatggaggt tgatgtgcgc ggcggtgttt
     8761 tgcgtttcgg gattgaggag ggcgcggcgg atgccgtctg aaagcaggca ggcgacggcg
     8821 ggatgttgct cgaaggcggt aacggccagc ccgagcgagg cgaggacgaa gctgtcgcgc
     8881 cccaatcctg cggttgcgtc ccaaacggtg gggtgcgcgg tgtggttgac ggctttggcg
     8941 atgagttcgc ccccgccttt tgtgcgccgg tattgtgccg cgccggaggc aaaatcgacg
     9001 atgacgctgc ttttttcccc gacaagcctg aggctgacgg tatcgtgttc ggcaaggagg
     9061 taactgcctt gttcgggcgg ttgggaaacg ggcacaaggg ggaatgcccg tatcagggtg
     9121 cggacggttt cggtggcggt gttgtcaata aggatgtcgg tcatggtgtt ttgtgtccgt
     9181 tcgggcgtgg ggcgaacccg ctttcccgtg ccgtttcaga cggcattttt tcgggtgggc
     9241 ggagatggtg cggatgccgt tttgtcgtca gccgatgttg aggcgttcca tacggtagcg
     9301 catggaacgg aagctgatgc ccaagcgttt ggcggcctgc gtgcggttgc cttcggtttg
     9361 tttgagggtt tgttcgatga tgtcgcgttc gatttggtcg agatagtcct gtatctgcat
     9421 ggtatcgggg tcgaacgcgc cgcaacgcaa cgcttcctgt gcctgatcgg cgttgccgaa
     9481 ggcggtgatg acggcaaccg gcgtatcgag caggcggctg ttgatgtgtt ggacgacttc
     9541 cagccccgag ccgtccggca tacgcatatc ggtcaggacg agcgaatagc gttggctgtc
     9601 gagcttgttt ttggcttcgg caacgcctga cgcggtatgg acgcgcaagc ccattttcat
     9661 cagggtcatt tccatcaggt cgagaatgtc ggtttcgtca tcgacgacga ggacggggtg
     9721 ttgcagttca gtcattggtt ttttccggta atgtgagttc gaaacatttg gcttccggta
     9781 ggtaggtcaa atcgccgaaa ttggcgtgcg ccagttcgcg ggcgacatac agcccgaggc
     9841 cggtgccgtt ttccgccgtg gtgtaaaagg gttcgaacag gtgttcctgc acttcgggcg
     9901 gtcggcaaag aggatacaga cggtgttttt ttgcgcgggg cggatggtga cggaaatcga
     9961 gccgggctgt ttgcggctgt gccgccacgc gttgttggcg aggttccaca taatttgccg
    10021 caggtgggcg ggatcgaaat aggcggtcgg gctgccgcct tgaatgtccg gacggatgca
    10081 gtcggcggca tcgggatggc cgagcaggaa ctcttgtttg aattcttccc aaaacggtat
    10141 caggccgatg gtttcgcgtt cggttttgtt gcgcttgttg agcgaggaaa tgtcttcgag
    10201 cattttgtcg atgcggcaga tgttgccgtc gatgattttg cacaatttgg cgttgaacgg
    10261 atctgccgcc cccgcttcca tattttcgcg cagcaggtcg ttggcgtggc ggatggcgga
    10321 catcgggttg cggatttcgt gggcgaggtt ggcggtcagt tgtccgagcg cggcaagttt
    10381 gacggacagg gcttctgcct gaatttcgct ttgcgggcgg atgtagagga tgagcagctt
    10441 gtccgaccct ttgttgaccg gaacggcgcg gatgccggcg ttcaggccgg gtgtgccgag
    10501 gacgtattcg aaagtgtgcg gagaggcttg atcccacagt tcggcggcag aatcggacag
    10561 accggcgcgc cgtccgattt cgagcgcggg gaaaaggtct ttcgccttcc ggttgaacag
    10621 caccgccagc ccttcggcat tgatgacgac gacagcttcc tgaacgcgtt cgagtacggt
    10681 ttggctcaag cctttgatgc ggtggtaggc gatatggctg tcgtaggcga gttttccggc
    10741 acggtcgatg tatttgaccg acagtgaggt gaagatggcg gaaagatagg aggcggcgac
    10801 aaggatgaag gttgccgagg cggttcgggc atccgatatg agcgggtatt tgccgatatc
    10861 gccgtcggca atggcgttga atatcagcag gatggcggcg tagctggaat agagcagggg
    10921 gtagcgcccg tagctgagca ggcaggagca gacgacgaag ggcaggatca ggatgccgaa
    10981 gccggaatcg atgccgccga acaggtaggt cagcacgccg atcatcgtga tgtccgccac
    11041 tgcgctgaaa cgcggcattt tcagcgactg ccattgccaa tgcggatgga taatggaaaa
    11101 gaaaatcagc cagcaggcaa tggcgagcca aacataaaac gcccacgccg tccacgaagc
    11161 gtgcaggttc gcacggctgc cggtttccag cccgaggatg tgcataatca tcagcgggaa
    11221 aacgatggcg acgcggatga tgttgatcag attgggaatc cggtctttga gtttttcaag
    11281 ttcgcggggg ttggaaatta ccataattat gtgcggaatc gggtttattt cggagaagaa
    11341 agctgtttca ggctgcccga tatgggcaat agtttgcctt ttttgccgcg ttttgcctca
    11401 agcagggaga tggggatgcg gtctttgtgc gctgcgccgc gcctgccttc gctttcaatc
    11461 aggatttctg cctcggaaga aacggcggta tgcgtcatcg attcgccggc gtttaatccg
    11521 atgatttgca gtcctttgcc ttttgccata attttcaatt cgccgatggg gaaggcgatg
    11581 gcgcggtttt gactggtggc ggcaatgatt ttgcaatcgg ggttgatgaa cgaggaggca
    11641 tagacggcaa cgggcggcag gacggtttcg ccactgtctg cggtcatgac cactttgccc
    11701 gctttcacgc gcccgaccat atcgcccagt ttggcaatga agccgtagcc gccgctgctt
    11761 gataataaat aatgttgttc cggcaatcct gtcaacatcg caacgggttt cgcgccgttt
    11821 tgcaactcga tcaaggatga aaccggcacg ccgtcgccgc gtccgccggg aatttcggcg
    11881 gcatcgagtg tataggttct gcccaatgaa tcgaggatga cgacgggtaa aaccgttctg
    11941 ccttcaaggg tttgtttgag gcggtcgcct tctttgaacg cggtttggct caaatcgaga
    12001 ttgtgtccgg cacggctgcg tatccagcct ttttccgaca gaatcagtgt gatgggttcg
    12061 tcggcggtgg tttgtgtcag cacggcgcgt ccggcctctt ccaccagcgt gcggcgtgca
    12121 tcgccgtatt gtttcatatc cgcctgcatc tctttgataa tcagcttgcg cttttcgttt
    12181 tcatcgccca aaaggatgtt cagacggcct tgttcttcgc gcaattcgtt caattctttt
    12241 tcgagtttga aaccttccaa acgtgccaac tggcgcaggc ggatttccaa aatgtcttcg
    12301 gcttggattt cggtcagccc gaacgccgcc atcaaatcgg ctttcgggtc atctgattcg
    12361 cggatgactt taatcacttc gtcgatgtgc agaaagactt tcagacggcc ttcgaggatg
    12421 tgcagccgtt tttccacttg gttcaaacgg aatttcagac ggcgtgttac ggttacggtg
    12481 cggaaatcca gccattcctg caaaatcgtt ttcaggtttt tctgcgcggg gcggttgtcc
    12541 agccccatca tcaccaagtt catggacaca ttgccttcca gcgaagtttg cgccatcagc
    12601 gtgttgatga aggtatcggt atcgatgcgg ctggatttcg gttcgaacac cagtcgcacg
    12661 ggatgttcgc cgtcggactc gtcgcgcacg cggtcgatta aatccagcat cagctttttg
    12721 gtgttgagct ggtcttggtt gagctgtttc ttacccgctt tcggtttcgg gttggtttgc
    12781 tcttcgattt cggcaaggat tttggcggaa ttggcgttcg gcggcagctc ggttacgatg
    12841 acgcgccact gtccgcgcgc caatttttcg atttcataac gcgcacgcac gcgcacgctg
    12901 cctttgccgg tttcgtaaat acggcgcaat tcgtccgccg gcgtgatgat ttgaccgccg
    12961 ccggcaaaat caggtgcggg aatgtattgc atcaggtcgg cggtttccag cgtcggcttt
    13021 ttcaacaacg caatcgccgc ctgcgtcact tcgttcaaat tgtgcgacgg aatctcggtc
    13081 gccatgccca ccgcaatgcc tgacgcgccg ttgagcaaca ccataggcag gcgggcgggc
    13141 aggtgcagcg gttcgtcaaa cgcgccgtcg tagttcggca caaaatccac cgtcccctga
    13201 ttgatttcgg acaacagcaa ttccgcaatc ggcgtcagcc gcgcttcggt gtaacgcatc
    13261 gccgccgccc cgtcgccgtc gcgcgagccg aagttgccga tgccgtcgat taagggatag
    13321 cgcaaggtaa aatcctgcgc catccgcacc atcgcctcat aggcggaact gtcgccgtgc
    13381 gggtggtatt tacccaaaat ctcgccgacc acgcgcgccg atttcaccgg cttcgccccc
    13441 gccgtcaaac ccatatcgcg catggcaaac aaaatgcgcc gctgaacggg cttttggccg
    13501 tctgaaactt caggcagcgc gcggcctttg accacgctca tggcgtattc gagataggcg
    13561 cgttcggcgt atcggccgag catcagcgta ttggaatcgg tatgtggagc gtgcggttgc
    13621 atatttatcg tatttccttt tcggttgaac ccccgccact tggacatctg tccttcgggg
    13681 cggtagaatc agactttatt tgggaggggg caaccccttc caaatcaggg caacacatag
    13741 ggcggtgctt tatgtgtcgt cctgtgtgtt gaaacattgt gtatgcggaa tgtaaagcaa
    13801 agggcattat tgtaaatcaa aacggcaaac catagtgggc gcgtgtctga atctgaatca
    13861 aatacgggaa tgtgaaaata tgttataaat aaggctttcc cactttcaca cattggagac
    13921 gatatggaat tggtatttat ccgccacgga caaagcgaat ggaacgcgaa aaacctgttt
    13981 acaggctggc gcgacgtgaa gctgagcgag caggggcttg ccgaggctgc tgccgccggt
    14041 aaaaaactga aagaaaacgg ctatgagttc gacatcgcct tcacatccgt cctgacccgc
    14101 gcgattaaga cctgcaacat cgttttggaa gaatccgacc aactgttcgt accgcaaatc
    14161 aaaacatggc ggctgaacga acgccactac ggccgactgc aaggcctgga caaaaaacaa
    14221 accgccgaac aatacggcga cgagcaagtc cgcatctggc ggcgcagcta cgacaccctg
    14281 ccgccgcttt tggacaaaga cgatgagttt tccgcacaca aagaccgccg ctatgcccat
    14341 ctgcctgccg atgttatccc cgacggcgaa aacctgcaag taacgctgga gcgcgtattg
    14401 ccgttttggg aagaccaaat cgcccccgcg attttgagcg gcaaacgcgt cttggtggcg
    14461 gcgcacggca actccctgcg cgcgctggca aaacacatcg agggcatttc cgacgaagac
    14521 atcatgggct tggaaatccc gaccggtcag ccgttggtgt acaaattgga cgacaatttg
    14581 aaagtcatcg aaaaattcta cctgtaaggg tgtgaaataa aaaatgccgt ctgaaggctt
    14641 gagcgttcag acggcatttt tgacggtaaa cgtagcaact gctttcgcga gaacgacggg
    14701 attttaggtt tttgtttttc tgttttcgtg ggaaggatgg gttttaggtt gcggtcattt
    14761 atcggaaaag cagaaaccgc tccgccgtca ttcccgcgca ggcgggaatc tagaacctct
    14821 ga
//
LOCUS       DAWFKN010000050        14673 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFKN010000050 DAWFKN010000000
VERSION     DAWFKN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14673
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.050"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(205..1056)
                     /locus_tag="WGR46_001402"
     CDS             complement(205..1056)
                     /locus_tag="WGR46_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0856327.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1397..2911
                     /gene="katA"
                     /locus_tag="WGR46_001403"
     CDS             1397..2911
                     /gene="katA"
                     /locus_tag="WGR46_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0856328.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            4245..4673
                     /locus_tag="WGR46_001404"
     CDS             4245..4673
                     /locus_tag="WGR46_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0856329.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSDKGTFPKNNASAGIASASEITGKYVAKVEVSGATITSTMKSQG
                     VNKDISGATLVLEGKQNSGSFEWTCKAGSVKAKFLPSICR"
     gene            5130..5630
                     /locus_tag="WGR46_001405"
     CDS             5130..5630
                     /locus_tag="WGR46_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856330.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            6106..6672
                     /locus_tag="WGR46_001406"
     CDS             6106..6672
                     /locus_tag="WGR46_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0856331.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(6948..8984)
                     /locus_tag="WGR46_001407"
     CDS             complement(6948..8984)
                     /locus_tag="WGR46_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0856332.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(9072..9944)
                     /locus_tag="WGR46_001408"
     CDS             complement(9072..9944)
                     /locus_tag="WGR46_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0856333.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPADSETL"
     gene            10214..12604
                     /gene="gyrB"
                     /locus_tag="WGR46_001409"
     CDS             10214..12604
                     /gene="gyrB"
                     /locus_tag="WGR46_001409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0856334.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            12703..14088
                     /locus_tag="WGR46_001410"
     CDS             12703..14088
                     /locus_tag="WGR46_001410"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0856335.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(14159..14257)
                     /locus_tag="WGR46_001411"
     CDS             complement(14159..14257)
                     /locus_tag="WGR46_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0856336.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(14302..>14673)
                     /locus_tag="WGR46_001412"
     CDS             complement(14302..>14673)
                     /locus_tag="WGR46_001412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856337.1"
                     /translation="VKEYQPEVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYDLVQTEYRRFKADKT
                     ENRNHKANSTFEYLHILEKTF"
BASE COUNT         3464 a         3977 c         3994 g         3238 t
ORIGIN      
        1 aaacccccgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg
       61 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      121 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct tttggtttgt
      181 tgtctttata agattattca gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      241 ctcaaaggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc cgcggaaagc
      301 cagcaggttg gcgagcgcct cgtcagaata cggcgtgggt gtggacgagg caaaaccagc
      361 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      421 ggtttggggc aggcgttttt ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct
      481 gtcgagggcg tttcggacga tatgcagggc ggagggtttg gcagcacatt cgcccgatct
      541 ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa
      601 ttttttcaat gcggtcaggg cttgtttcag cattgcttca tccaacatcc actccaaggg
      661 cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc
      721 gatgaggatg tggacacagg cggattcttg atcggaaagc gggtgttcgc atacttgcgc
      781 gtgcaggtat tgcttgaaat cctgctcgcc ggcgatgttg gacagcatat cttcgccttc
      841 gtctccgccg atttgacggg caggcgcagt gtaatggctg aactcggcat cggaaaattc
      901 atccgtatct ttgcgttcga gcagggggtt gtccgacagc cagttttcga cctcgcgttc
      961 aagttcgata cccgacatct gcaatacgcg caaagattgt tgcagccgct ggttgagctg
     1021 ctgggtctgt ttgagcttta ttccgagtaa cgtcatgata atgcgagaga attattattt
     1081 tggcctgtcg gtggcagatg atgccgggaa acattattat aaatggtttt atgtagggat
     1141 tttgattcgt gccgataaca aaaaacaaac gaaaatcaaa aaccgattgc ttataacaat
     1201 attaaatcga tttcatagtt ttaatagcga aaatcttggc gtatagtcgc atccatagtt
     1261 tttacaaaag ggaaataaaa tgtcgattca agaaatttat tgcaatcaag aaaccggtta
     1321 cgaatacgct ttccgccaaa tcgtactgta agccgtttac ccccatttga ccaacctgag
     1381 aaaaggaaca agagcgatga ctacctccaa atgccctgta acccatctga ccatgaacaa
     1441 cggcgcgcct gttgccgaca atcaaaacag cctgaccgcc ggtcctcgcg gccctctgct
     1501 ggcgcaggat ttgtggctga atgaaaaact cgccgacttc gtgcgcgaag tcatccccga
     1561 acgccgtatg cacgccaaag gttcgggcgc gttcggtacg tttaccgtaa cgcacgacat
     1621 caccaaatac acccgcgcca aaatcttcag cgaagtcggc aaaaaaaccg agatgttcgc
     1681 ccgtttcacc accgtggcag gcgaacgcgg cgcagccgat gcagaacgcg acatccgcgg
     1741 ttttgccttg aaattttata ccgaagaagg caactgggat gtggtcggca acaacacgcc
     1801 cgtgttcttc ctgcgcgacc cgcgtaaatt ccctgacctg aacaaagccg tcaaacgcga
     1861 tccgcgcacc aatatgcgct ctgccacaaa caactgggac ttctggacgc tgctgcccga
     1921 agcactgcac caagttacca tcgtgatgag cgaccgcggc atccccgccg gctaccgcca
     1981 tatgcacggc ttcggttcgc atacctacag cttctggaac gaagcaggcg agcgtttttg
     2041 ggtaaaattc catttccgca cccaacaagg cattaaaaac ctgaccaacg aagaagccgc
     2101 caaaatcatc gccgacgacc gcgaaagcca tcagcgcgac ctgtacgaag ccatcgagcg
     2161 cggcgagttt ccgaaatgga cgatgtacat ccaagtcatg cctgaagcag acgcggaaaa
     2221 agtaccttat catccgtttg acttgaccaa agtttggccg aaaaaagact atccgctgat
     2281 tgaagtgggc gaattcgagt tgaaccgcaa tcccgaaaac ttcttcgccg atgtggaaca
     2341 atccgccttc gcaccgagca acctcgttcc cggtgtcggc gccagcccag ataaaatgtt
     2401 gcaggcgcgt ttgttcaatt acgccgacgc gcaacgctac cgcttgggcg tgaacttccg
     2461 ccaaatccct gtcaaccgtc cgcgttgccc tgttcacagc aaccagcgcg acggacaagg
     2521 acgcgccgac ggcaactacg gcagcctgcc gcactacgaa cccaacagct tcggccaatg
     2581 gcagcagcag cccgacttcg ccgaaccgcc tttgaaaatc aacggcgacg cggcgcactg
     2641 ggactaccgc caagacgatg acgactattt cagccaaccg cgcgccctgt tcaacctgat
     2701 gaacgacgcg cagaaacagg cattgttcgg caacaccgcc gccgcaatgg gcgacgcgcc
     2761 cgacttcatc aaataccgcc atatccgcaa ctgctaccgt tgcgatccgg catacggcga
     2821 aggcgtggca aaagccctcg gactgactgt cgaagacgca caagccgccc gcgcgaccga
     2881 ccccgcactc ggccagcccg gtttgctgta agggggcatt atgtggatgg aaattgaaga
     2941 aatcctgtcc cgcgccgtcc gctaccggaa ataaccgggc ataaaaatgc cgtctgaaac
     3001 gtcgtttgac cgcttcagac ggcatttcca caccccaccc cgcattttta tatggtcgcg
     3061 catcgcagtg ggacgtaggg tgttcgtagg gtgggcttca gcccaccaat cacaccaatc
     3121 acaccaatcc gccacctcga caaatccgcc acctcgacaa atccgacaaa tccgacaatc
     3181 ttaccggtgc cggcaaacct atgccgtctg aagttggcgg aggcggtggg ctgaagccca
     3241 ccctacagcc cgccattccc gccccacagc caacttttcc gcgtcattcc cgcgaaagcg
     3301 ggaatctaga acgtaaaatc taaagaaacc gttttttccg ataagtttcc gtaccgacaa
     3361 ctctagattc ccgcctgcgc gggaatgacg aaatttttgt ttttgatttt ttgtttttgg
     3421 ggaatgacgg gatttgagat tgcgggcatt tatcgggtaa aacggaaatt aagcgttgcg
     3481 aaaatttatc cgaaatcaca gtaacttttc cgcgtcattc ccgcgaaagc gggaatccag
     3541 gttcgttgag tttcagccat ttctaataaa ttcttgcaac tttgagtttc tggattcccg
     3601 cctgcgcggg aatgacgaaa tttcagtttt tgattttttg tttttgcggg aatgacggga
     3661 tttgagattg cgggcattta tcgggaaaac agcaacctct cctccgtcat tcccacgaaa
     3721 gtgggaatcc aggttcgttg agtttcagtc atttctgata aattcttgca gctttgagtt
     3781 tctagattcc cactttcgtg ggaatgacgg gatacgggtt ttcgtgcgga cagatccgtc
     3841 attcccgcgc aggcgggaat ccggacccgt ccgcacggaa acttatattt cgtcattccg
     3901 ccaatcccac cccaccccca aaaaaaccaa aaaaaaccca atctgacaaa atctgacaaa
     3961 aaacgtcagc ccgaaactga caaaaattgt cacaattgac aaaaaaacgt cagcccgccg
     4021 caggccgcgc cgcgttgata taaaaaaata ccaataccaa acagacaaat aaaaaaatat
     4081 tctgtaaaat gccgagctga accccgtttt ggcacgccgc ttgctggaaa gaaggcgaac
     4141 cgaaacaaaa aagaccacgg tcaaatacat tacgggttta cgtttgcggc agatgccgca
     4201 aatttccaat caaacttaaa cattttcaaa caggagtcat ccaaatgaaa gcaatccaaa
     4261 aaggtttcac cctgatcgag ctgatgatcg tcatcgccat cgtcggtatc ttggcagccg
     4321 tcgccctgcc cgcataccaa gactacaccg cgcgcgccca aatgtccgaa gccctgactt
     4381 tggccgaagg tcaaaaaacc gcagtggtcg agtattattc cgacaaaggc acattcccga
     4441 aaaacaatgc ttccgcaggt attgccagtg cttctgagat tacaggtaag tatgtggcta
     4501 aggtagaagt ttcgggtgct accattactt ctacaatgaa aagtcagggt gtaaacaaag
     4561 acatcagcgg cgcaaccttg gtactggaag gcaaacaaaa ctcaggctcg ttcgagtgga
     4621 cttgtaaagc aggttctgta aaagcaaaat tcttgccatc tatctgccgc taataaggac
     4681 aatggtcggg tttgaccccg ccgtgaaaac aaatgccgcc cgaaccgctc gggcggcatt
     4741 ttctattccc ctttgccgct gtagggctgt agggtgaggc gtaggtgcgt agggtgggct
     4801 gtagggtggg cttcagccca ccattccacc aacccaacca actccaccac atcaaggatt
     4861 tttggcgaac cgttgggatt cggcgggttt cgagtttcgg cggggattgg cggaatggtt
     4921 ggtttgggtt gggtgggaat ggttggattg gcggaatggt gggctgaagc ccaccctacg
     4981 aaacacccta cgaaacaccc tacagcctac acatcagccc tacgccctcc ctacccattt
     5041 ccctgcaaat cccctaaaaa tcctcccgca cgccctgaaa acaaggaata atacaggttt
     5101 ccgcccatcg ggctaaaaag gggataaata tgtcaaatta ccggagggac ttcacgcgcg
     5161 gggcgcacta ttttttcaca ttggctttgc aagacaggac gaaggattac ctgatacggc
     5221 atatcgacag gctgcgcctc gcctatcgtg aaacgaaaac acgttatccg tttgaaacgg
     5281 cggcaatttg tattttgccg gatcacctgc atttcattat gcggctgccg gaaggcgacc
     5341 acgattaccc gatacggatt gcatatttga aaacccggtt ttcccaactc atccccaaag
     5401 agtgccgcgc ccccaacggg agtaaaacaa aaaaacgcga agccggcatt tggcagcgca
     5461 ggttttggga acacctgatc cgggacgaac aagatttaaa caaccaccgg gattatattt
     5521 atttcaaccc cgtgaaacac gggtatgcgg cgagtgtgaa ggaatggcgt tattcttcct
     5581 tcttcaaaga cgttgaaaaa ggcatttacc ctttggattg gggagggtaa agctcccgcc
     5641 gccgccaaaa tccgcaggac ggacgcgggg cagatgagtc gggcgggacg tagggtgagg
     5701 cgtaggtgcg tagggtgggc tgtagggtgg atgtgtaggg tgggcttcag cccaccaatc
     5761 caaccacctc cgccaatcca accattccca ccaacccaaa ccaaccattc cgccaatccc
     5821 cgccaaaaac cgaaaccgcc gaatcccgac ggtttgccaa aaacccttga tgcggtggaa
     5881 ttggcgggat tggatgagtt ggtttggcag aattggtggg ctgaagccca ccctacagcc
     5941 caccctacaa cccgccctaa gtcccaccta cgttccatcc gacgcatccg ccctacggtt
     6001 ccggattccc ctgcgaaccg ccccgcgcac gaaaactgct ttacgcggcg tttttcgcgt
     6061 tttgctgttt tgctataatc cgcccttttt aaggacgggt gcggtatgag tctttacgct
     6121 ttgctcttga ttgcgctggg gatgtcgatg gatgcgtttg ccgtcgcgct ggcaaagggt
     6181 gcggcggtca ggatgcctcc gcgcaaaatt gcagcaacag ccttggtgtt cggcacggtt
     6241 gaagcgctca cgccgctggc aggctgggta ggcggttttt atgccaagcc gtttatcagc
     6301 gaatgggacc attgggcggc tttcgtcctg ctgggcgggc tgggtctgaa aatgatgcac
     6361 gaagggttgt ccggcgaggc ggaggacgcg cgggaaaaca aacgggaaag ctggtggttg
     6421 acggttctga ctgcttttgg aaccagtatt gattccatga tagtcggggt gggcttggcg
     6481 tttatggagg taaacatcgc ctttgccgcc gcaatcatcg gtatggcgac gacggtgctg
     6541 gtggcggtcg ggctgacggt gggaagggct ttgggcgtat tgttcggcag gcgtgcggaa
     6601 tttgccggag gtttggtgtt gattgccatc ggtacatgga cgctcttgtc gcacttgggg
     6661 ctgatcgggt aaggactgcc aaaaaatgct gtctgaagcg ttgtttgacc gtttcagacg
     6721 gcatttttta ttttttgggc gtagggcgaa tccgtaaggt gggatgtaag gtgggatgta
     6781 gggtgggctt cagcccaccc tacgcccact gtttttgatt ttttgttttt gcgggaatga
     6841 cgggattttg gcttactgtt tttgtgaaaa tggcgaaacc gcgcaaatgc tgatgccgtc
     6901 tgaaacactg tttgattgtt tcagacggca ttttttgttc acatcgatca agccgcgttg
     6961 tcgaaaccgc tgtggcgcaa gagtgcgtct atgctcggtt cgcggccgcg gaaggctttg
     7021 aaggattctg ccgcgctgcg cgatccgccg acggcgagga tttcctgcca aaagcgtttg
     7081 cctgtggcgg cgacatcgtc gctttcttca aaggcggcgt atgcgtccgc gctcaatact
     7141 tccgcccacg cgtagctgta atagcctgcg gaatagccgc ctgcgaagat gtggccgaag
     7201 ctcaaggcga agcggttgta ttcgggcggc tggatgacgg cgactttttt gcgcacgctg
     7261 tctaaaacct gttgccagtt tttcagacgg ccttcgtcgt cttcgctgta aatcatcata
     7321 tcaaagaggg cgaactccat ttgccggacg aggaacatgc cgcgttggaa gtttttggcg
     7381 gcgagcattt tgtcgaagag ttctttcggc aggggaacgc cggtttcttc gtgggctgac
     7441 atttgtgcca agacattgta ttcccaaacg aaattttcca taaactggct gggcagttcg
     7501 accgcgtccc attctacgcc gttgatgccg gatacgccca gttcgtccac ttgggtaagc
     7561 aggtggtgca gcccgtgtcc ggtttcgtgg aagaggatga ggatttcgtc gtggctcagg
     7621 cgggcttccc tgccgccgac gggtggggcg aagttgcaga cgaggtaggc ggtgggcagt
     7681 tgcagcgtgc cgtctgaaaa acggcggcgg cctttgtagt cgttcatcca cgcgccgccg
     7741 cgcttgcctt cgcgggcgta gaggtccata tagacgccgc ctatggtttt gccgttttgt
     7801 tgcaattcaa aatagcgcac gtctttgtgc cagacgggga cggttttttc ggtaaatccg
     7861 atgccgtaga gttttttgat ttgggcgaac agtccgttta atactttgcc gacggggaag
     7921 tattttttga cttcggtttc gctgaacgcg tatttggctt cgcgcagttt ttcgctggcg
     7981 tagcccaagt cccacggttg caaatcggcg aggccgaggc tttcgcgggc gaaggctttg
     8041 acttcggcga ggtctttttc ggcgtagggt ttggcgcggc gggcgaggtc gtgcaggaag
     8101 tttaaaactt gttcgggggt gtccgccatt ttggttgcca gcgacaattc ggcgtagttt
     8161 ttgaagccga gcagtttggc ggtttgcagg gcgtttgcga gcgtgcggtc gatgttggcg
     8221 gtgttgtcga atttgccgtc gtctgacagt tcgctggcgc gggtaacgta ggcgcggtag
     8281 atttgttcgc gcagttcgcg gttgtcggcg tattggatga cggcgaggta gtgtggaatc
     8341 tgcaagccga ttttgtagcc tgttttgctt tcgctttgcg cggcggcggc aaacatggcg
     8401 agcgcgtctt cgggaatgcc ggcaagcggt gcggcatcgt caaagtaaat gccgaacgcg
     8461 tcggtcgcgt ctaggacgtt ttgggagaat ttggcggaaa gttgcgcgcc ttcggtttgc
     8521 agttttgcca gttctgcctg ctgttcgggc ggcagttccg cgccgctgag gacgaaatcg
     8581 cgcagatcgt ggttgagttt ggttttttgt gcgggggaga gggtgtcgaa ttcgggggaa
     8641 tttttgatgg ttttgaagcg gttgtacagc tcgatgtctt gtccgatttc ggtgaagaag
     8701 acggtgattt cgggcatcag ttcgttatag acggcgcgca gttcgggcgt gtcggcgacg
     8761 gagttgaggt gcgacaccac gccccaaatc ctgccgacgc gttcggtgat gccggtcagg
     8821 ggttcgacag tgtttgccca gccggtgtgc gtttgggctt tgatggcggc gatttgttcg
     8881 cgcgcttcgg cgatggcggt ttgcagggcg ggtttgatgt cttcggtttt gatttgatca
     8941 aaacggggtt cttcgcccaa atggagcagt gcgttgtcag tcataagatg ggtttccttt
     9001 gcgtgttgtg cttcagacag catatcggca atatgccgtc tgaagccgga aatggggtcg
     9061 gtcggggaaa gttaaagtgt ttcggagtcg gcgggatgat ttaacaggcg gtattgtacg
     9121 cgcaaaaatt cgtccataac cgcctgttgg atttccagcc gctttgaaac gggggaggcg
     9181 aagcggacga tgatgcggta tgccttgtcg tcatacggta cgcgggtaac gcgcggtctg
     9241 gcggcgggcg tgataaacag tttttccgcc tgcacgtttt ccaaatgccg ctggatggcg
     9301 gggatgtagg gcgcgcacaa gggctcgagt acggctttca gacggcatac ggcctcatcc
     9361 gaatccaaat ggatggggac ggggatttcg accgtatgga tgacgtagtc gcccaaaata
     9421 ttgtcgcggc gcacggggtg gcttaacaac aggctgttgg ggaaagaaac ggtggttccc
     9481 gcaagctgtc cgaccaaggg gttcgggccg acctgcatca tcagcgtgtt caacaggttg
     9541 atgtcgacca cgcgcccgcg caggccgttg atttcgatat agtcgccgac cgagtattgc
     9601 tgggtggcag accttaaaat actgcccgac agacacataa tcagttcctt cgtcgccacg
     9661 acgaccgccg ccgccaccgc aaacatcgac aaagccagcg tttgaatttg cgccgaccag
     9721 ataaatgcca gcgaaaacag caccaaaagc agcgttatat tgcggctggc aaccaaaaac
     9781 cgccgcttgc tttcgatgcc gaaatccgga tgccgtttga agtggatatt caacagaagg
     9841 gcgcgcgcca gcagcaaagc cgcaaccgcc gccacggatt cgaccgcctc cgcacgtatc
     9901 gggacggcac cgagccaagt gtccaacata ttccatattt ccatttccgc gcccctgtcc
     9961 gaaaagtata gggtgtagat tttagtggca aaaaaacgct tttgccactt tacgggacaa
    10021 tccccgacct gatgccggag gcaaacgtac cgtttcatct tttcccggcg gcacactgat
    10081 gcggataacg ctgaattagg cggcaaaatc ggctataata cacaattata tatgtcgtcc
    10141 cgcccgcttt ttctacaagg tgcggacttg ttttggcggc atcgcaaaat cttttcaaaa
    10201 tccggcaaaa aatatgactg aacaaaaaca cgaagaatac ggcgccgaca gcattcaggt
    10261 gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg tacatcggcg acacgcagga
    10321 cggcagcggt ctgcaccaca tggtgttcga agtattggac aacgccattg acgaagcact
    10381 cgccggacat tgcgacaaaa tcacggtaac gatacacgcc gaccattccg tcagcgtcgc
    10441 cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa gaaggacgct ccgccgccga
    10501 agtcatcatg accgtattgc acgcgggcgg taaattcgac aacaacagct acaaaatctc
    10561 cggcggcctg cacggcgtgg gcgtgtccgt cgtcaacgcg ctgtccgact gggtaacgct
    10621 gaccatctac cgcgacggca aagaacactt cgtccgcttc gtgcgcggcg aaaccgaaga
    10681 gccgctgaac gttgtcggcg attccgataa aaaaggcacg accgtgcgct tcctcgccag
    10741 tacggaaacc ttcggcaacg tcgaatacag cttcgacatc cttgccaaac gcatccgcga
    10801 actttcgttc ctgaacaacg gcgtggacat cgaattgacc gacgagcgcg acggcaaaca
    10861 cgaaagcttc gccctttccg gcggcgtggc tggattcgtg caatacatga accgcaaaaa
    10921 aacgcccttg cacgaaaaaa tcttctacgc gttcggcgag aaagacggca tgagcgtcga
    10981 atgcgcaatg caatggaacg acagctatca ggaaagcgtg cagtgcttca ccaacaacat
    11041 ccctcagcgc gacggcggta cgcacctgac cgcgttgcgc caagtgatga cgcgcaccat
    11101 caacagctac atcgaagcca acgaagtcgc caaaaaagcc aaagtggaaa ccgccggcga
    11161 cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa ctgcccgacc ccaaattttc
    11221 gtcccaaacc aaagacaaac tggtttccgg cgaaatcggt cccgtcgtca acgaagtcat
    11281 cagccaagcc ctgaccgact tcctcgaaga aaaccctgcc gaagccaaaa tcatcaccgg
    11341 caaaatcgtc gatgccgccc gcgcgcgcga agccgcccgc aaagcccgcg aaatcacccg
    11401 ccgcaaaggc gtaatggacg gcttggggct acccggcaaa ctcgccgact gccaagaaaa
    11461 agaccccgca ttgtcagaac tttatttggt cgagggcgat tctgcaggcg gttccgccat
    11521 gcaggggcgc gaccgcaaat tccaagcgat tttgccgctt aagggcaaga ttttgaacgt
    11581 ggaaaaggcg cgctttgaaa aaatgctcgc cagtcaggaa gtcgccgctt tgattactgc
    11641 gttgggtgca ggtatcggca aggaagaatt caatgccgag aagctgcgtt accaccgcat
    11701 catcattatg accgatgccg atgtggacgg cgcgcacatc cgcaccctgc ttttaacctt
    11761 cttctaccgc caaatgcccg agctggtcga acgcggctac atctacattg cccagccgcc
    11821 tttatacaaa gccaaatacg gcaaacaaga acgatatctc aaagacgaat tggaaaaaga
    11881 ccaatggctg ctcggtttag ccttggaaaa agccaaaatc gtttcagaca gccgcaccat
    11941 cgaaggcgaa gagcttgcca ataccgccaa acaattcctg ttggccaaaa ccgtcatcga
    12001 acaggaaagc cgcttcgtag acgaactcgt cctgcgcgcc atgctacacg cgtcgcccat
    12061 tgatttgacg tcgtctgaaa acgccgataa agccgttgcc gaactttccg gtctgcttga
    12121 cgaaaaagaa gtcgccctcg aacgcatcga aggtcatgaa ggacaccggt tcatcaaaat
    12181 cacgcgcaag ctgcacggca atgtggtggt gagctacctc gagcctaagt tcctcaacag
    12241 caaagcctat caaaccctta cccaaaccgc cgccgcactt aaaggcatgg tcggcaaagg
    12301 ggcgaagctc tacaaaggcg acaacgaata cgacatcgac agctttgaag gcgctttgga
    12361 catcttgatg agcgttgccc aaaaaggtat gtccatccaa cgatacaaag gcttgggcga
    12421 gatgaacccc gaacagctgt gggaaaccac gatggatccc gccgtgcgcc gcctgttgaa
    12481 agtgcgcatc gaagatgcca ttgccgccga cgaagtattc gttaccctga tgggcgacga
    12541 ggtcgaaccg cgccgcgcct ttatcgaaaa caacgcgctg attgcccaaa atatcgacgc
    12601 ataagtgccg ttttaaaaaa ggagacgggc atcgtgccgc gtctcctttt ggtttgtcaa
    12661 acggaagcca tgccgtctga aaccgccttt cggagcaaaa ccatggtcag catttttgat
    12721 attttcaaaa tcggtatcgg gccttccagt tcgcatacgg tcggcccgat gaaggcagcc
    12781 gccgcctttg cggcaggttt ggatgcacag gctgttcgca tcgtcatcga catttacggc
    12841 tcgctcgccc tgaccggata cggacacggt acatttgacg cgctgatgct cggtttggaa
    12901 ggcagcctgc cgcacgacat cccgcttgtc ggcattcccg aacgcctcga acgcatccgc
    12961 acgcagcaca tcctccggct caacgggcaa gaaatccgct tcatccccga ccgcgacctg
    13021 aacatactcg gcaatcaagt gctgcccaaa caccccaacg gcctgcgttt taccgcctat
    13081 gcttcagacg gcacggtatt gaatgaacag gtttattatt cggtcggcgg cggctttgtc
    13141 gttaccgaag aagattttga ccggcaggcg gaaacggaaa aagccgttcc ctatccctat
    13201 accagttgcg ccgaactgct tgcccgatgc cgtctgaacc ggctcgacat ctccgaagtc
    13261 gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag ccgaaatccg ccgccgcgcc
    13321 gctgccgttg ccgaggttat ggaaggctgc atcaaacgcg gcttgggtgc ggacggcgaa
    13381 ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc ttgccgccaa gctcaaagtc
    13441 ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga tggtgtacgc catggcggtc
    13501 aacgaagaaa acgccgccgg cggacgcgtc gttaccgccc cgaccaacgg ggcggcaggc
    13561 atcattcccg ccgtattgca ctatttccgc aagttcaacc cccacgccac acaggaacgc
    13621 gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc tctacaagac caacgcctcc
    13681 atttccggtg cggatgtcgg ctgtcagggc gaagtcggcg tagcgtgttc gatggcggcg
    13741 ggcgcatacg ccgaagtcat cggcggcacg cccaaacaag tggaaaacgc cgccgaaatg
    13801 gcgatggaac accatttggg gctgacttgc gaccccgtcg gcggactggt gcaaatcccc
    13861 tgcatcgagc gcaacggcat cgccgccgaa aaagccctca aactcggcac gctcgcgctt
    13921 ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag tcatccgaac catgctgcaa
    13981 accggccggg atatgaaggc gacctacaaa gaaacctcgc ttgccggact cgccgccacg
    14041 ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg agtgctgagg atggacagaa
    14101 acgaaaatgc cgtctgaaaa cagtttttta gacggcattt catttatttc ttgcggcttt
    14161 accgtttcag tgccagcgtc aacacgcccg ccgtaaccag ccccaagcct atccattcct
    14221 gcgtgttcgg gcgttcgtct aagaaaacca ccgccatcag gaatgcaaac cggcgttgtt
    14281 tgcagcatcc ggcaagaatt tttaaaaggt cttttctaaa atatgcagat attcgaatgt
    14341 cgaatttgcc ttatggttac ggttttctgt tttatctgcc ttaaaccgcc ggtattccgt
    14401 ttgaaccaaa tcatatttgc cgaaacgctc aaaaatctcc cttacctgcc cgacagacat
    14461 caagccttcg ttgttgtagc tgagaaaaat atattgaaag tccgcatccc taatcaaagc
    14521 ctcaaatacc ggaaccacat ccgattttga gcaagtccgc gctaaaacac atcgtacagc
    14581 gcaaaaccgg atgggaatgg caaaatccgg aaacaaacat ttttcaaagc atacaaacaa
    14641 aaaaatacca acctccggtt ggtattcctt aac
//
LOCUS       DAWFKN010000051        14177 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFKN010000051 DAWFKN010000000
VERSION     DAWFKN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.051"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..82)
                     /locus_tag="WGR46_001413"
     CDS             complement(<1..82)
                     /locus_tag="WGR46_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0856338.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHII"
     gene            complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGR46_001414"
                     /pseudo
     CDS             complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGR46_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGR46_001415"
                     /pseudo
     CDS             complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGR46_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2152..4425)
                     /locus_tag="WGR46_001416"
     CDS             complement(2152..4425)
                     /locus_tag="WGR46_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0856339.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKVSDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4489..5139)
                     /locus_tag="WGR46_001417"
     CDS             complement(4489..5139)
                     /locus_tag="WGR46_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0856340.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5136..5933)
                     /locus_tag="WGR46_001418"
     CDS             complement(5136..5933)
                     /locus_tag="WGR46_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0856341.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5933..7096)
                     /locus_tag="WGR46_001419"
     CDS             complement(5933..7096)
                     /locus_tag="WGR46_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ0856342.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGR46_001420"
     CDS             complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGR46_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0856343.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8493..9611
                     /gene="wecB"
                     /locus_tag="WGR46_001421"
     CDS             8493..9611
                     /gene="wecB"
                     /locus_tag="WGR46_001421"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ0856344.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            9613..11250
                     /locus_tag="WGR46_001422"
     CDS             9613..11250
                     /locus_tag="WGR46_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ0856345.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            11323..12066
                     /locus_tag="WGR46_001423"
     CDS             11323..12066
                     /locus_tag="WGR46_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0856346.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            12068..12931
                     /locus_tag="WGR46_001424"
     CDS             12068..12931
                     /locus_tag="WGR46_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0856347.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            12976..13995
                     /gene="galE"
                     /locus_tag="WGR46_001425"
     CDS             12976..13995
                     /gene="galE"
                     /locus_tag="WGR46_001425"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0856348.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
     gene            14111..>14177
                     /locus_tag="WGR46_001426"
     CDS             14111..>14177
                     /locus_tag="WGR46_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ0856349.1"
                     /translation="MRKILVTGGAGFIGSAVVRHII"
BASE COUNT         4184 a         3205 c         2846 g         3942 t
ORIGIN      
        1 gaatgatatg gcggacaacg gcagagccga taaagcccgc gccgccggta acgaggatgg
       61 tttttttgtt tgcggtttgc atttaatccc catatccgtt ggggttgcgg ctgacccaac
      121 gccacgaatc ctccatcatt tgcgcaaggc tgcgtttggt ttcccaacct gtctcttgtt
      181 tggtatggga cgggtcggca aaggaacacg ccaagtctcc ggcgcgtcgg ggttggattt
      241 gatagggaat atttaagccg gatgcggctt caaaggcacg gatgatttcc aaaaccgaat
      301 aggcgcgccc cgaacccaag ttgaacaaat gtacgccggc aacaccgcct ttcgccttca
      361 ttgccgcgat atgcccttct gccaaatcca tcacatgaat atagtcgcgc attcccgtac
      421 cgtccggggt cggataatcg ccgccgaata ccgacagttg cggcagcctg cccgaagcca
      481 cttggcagat atagggcaaa aggttgttgg gaataccgtt gggctgttca ccgatcaagc
      541 cgctttcgtg cgcgccgatc gggttgaaat agcgcaacaa aatcacgctc caacgcggat
      601 cggccttttg gatgtcggtt aacatccgtt ccaccatcgc tttggatgcg ccataaggat
      661 tagcggtatc gcccgggcgc atatcttccg tatagggcat tttacgtatc atggcgcgac
      721 gcattaaagt aaacgccaaa gcgcgtttat actggttatc cagtttccct atatttttac
      781 gttacaaatc aagttgttgg tattcatcgt gaaaatagaa tttttcggca taacgctgac
      841 tcataaaacc ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg
      901 tttcatggtt gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt
      961 tagtaatcac acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa
     1021 atacatctgc aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt
     1081 tctctttatt accgatatag ttaattttcg gatattgctt attcattttc actcattttt
     1141 aaaatacttt cagcaatagc ttttgccaat aaaggcggta cggcgttacc aacctgctgc
     1201 tgctgggcaa ttttgctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct
     1261 aattcacgaa cggttaacgc ccgattctgt tcatagtgaa aaactttgcg catatctcct
     1321 gtaatacaaa cggctggttt tgttgctgtt gtaacggatg tatttacgga tatcacctgt
     1381 tttcggacgt aatggttcag gaatatcgtt acggttacct ccatttttaa caaatgccat
     1441 tttttctaac atttgtgccg aatgattcat agcttcatga tttgcaacgt gtggattgct
     1501 ttcgccagca gccagttttg gaaaatgtcc tattgctgat ccaacagtct gatgggaaat
     1561 ctgcaaaggt tcgggaaagg aaattttgcc tttatccctc ctcccgataa atatcactcg
     1621 gctacgtatc tgaggaacac cgaaatcggc tgcactcagt atcttacatt ccaccgaata
     1681 accgatattc tgaaatgctt gaataatctc aatacgtgtt ttacctgaat tgtgtgtata
     1741 gagtcgcgct acattttcca taacaaaaaa atatggttgg acaattttaa ctattcggac
     1801 aaactcttta aataaatggt tgcgtgggtc atctgtaaat gtccgtccaa tctttcctgc
     1861 catactaaaa ccttgacaag gtggtcctcc aataatcaaa tcaactgctt gtccgttaag
     1921 acaattgatt aaatcttgtt cggttagtgt ggttaaatct ttttgcagta attgatgatg
     1981 ggggaagttg gtacggtaag tctgacaata atcagactcc atttcaacag aaagcaattg
     2041 ttggaatccg gcttgttcaa aacccaagga taggcctcct gctcctgaga aaaggtcaat
     2101 ataggtaagt ggtgtgtgca taagtcaaaa tccataaact cttcgattat tttaccgctt
     2161 cagcttcgca aacgcatccg ccatcgccga attggctggg gcgcggtcgt tgcgttgggg
     2221 tttgcggccg gctgtccgtt cgcggctgcg gttttcagac ggcattttat gttttgcgcc
     2281 gcccggttcg tcatccaagc gcatggtcag cgcgatgcgt ttgcgtgcag catcgacttc
     2341 cagcactttc actttcacca cgtcgccagc tttcaccact tcgcgcgggt cttggacgaa
     2401 cttgttggac agggcggaga tgtgcaccaa gccgtcctga tggacgccga tgtccacgaa
     2461 cgcgccgaag ttggcgacgt tggaaaccac gccttcgagt atcataccga cttgcaagtc
     2521 gctgatttcg tggatacctt cggcaaacga tgccgtctga aactcgccgc gcggatcacg
     2581 gccgggtttt tccagttcgg acaggatgtc caaaatggtc ggcaggccga agcgttcgtc
     2641 ggtgaagtcg gacactttga tttgcttcac gcgctcgcgg ttgccgatga gttcggcggc
     2701 gctaatgcct tgttgcgcca gcattttggc gacgacggga taggcttcgg ggtggacggc
     2761 gctcgcgtcc aacggctctt taccgccgtt aatccgcaaa aagcctgccg cctgctcgaa
     2821 ggttttttcg cccaaacgcg gcactttcag caattttttg cggctgtcga acgcgccgtt
     2881 ttcatcgcgg taggcgacga tgttttgggc aagagtttga ttcaaaccag aaatccgcgc
     2941 caagagcggg gcggaggcgg tattcacgtc cacgccgacg gcgttcacgc agtcttcgac
     3001 caccgcgtcc agcgatttgg cgagccggct ttggttcacg tcgtgctgat actggcccac
     3061 gccgatggat ttggggtcga ttttgaccag ctcggcaagc gggtcttgca gcctgcgggc
     3121 gatggacacc gcgccgcgca gggaaacgtc caagtcgggg aactcgcgcg ctgccagttc
     3181 ggacgcggaa taaatcgacg cgccggcttc ggacacgacg attttatgca gccccatttt
     3241 cgacatttca cgcaccagtt cgcccgcgat tttgtcggtt tcacggctgg cggtgccgtt
     3301 accgatggcg atgagcttca cgccgtgctg tttaatcagg cgcgacagcg ttgccaacat
     3361 attgttttct tgatgcaaat agacgatgac ggtatccagc agcttgccgg tgtcgtccac
     3421 cacggcgcat ttcacgccgt tgcggtagcc ggggtcgaga cccagcgtgg tcagccgtcc
     3481 ggcgggcgca gcaagcagca agtctttgag attgcgggcg aacacggtaa tcgcgtcggt
     3541 gtcggcggct tctttcaaac gattgagagc ttcaagttcc aacgacaaaa agattttcgc
     3601 gcgccaagtc aggcgcacgg tgtcgcgcag ccatttgtgg ccgtctgaaa ctttgaagcg
     3661 gcgggcgatg atttgctcgt attcgctttg ctgcgtaatc ggcgtgtcgt cgggctgata
     3721 tttgagcgcg atgttcaaca cgccttcgtt gcgtccgcgc aaaaccgcta gcgcacgatg
     3781 gctgggcatc gcacgaatag gttcgcggtg gtcaaaataa tcgctgaatt tttcgccttc
     3841 ggtttctttg cctccaacaa cttgcgcgtg gatttcggct tcgttccaca gcttgtcgcg
     3901 cagcgtgccg atgagttccg cgtcttcggc aaactgctcc atcagaatcg cacgcgcgcc
     3961 gtccaacgcg gctttggcat cggggacgtt ttcgttcagg tagccttgtg cggcggcttc
     4021 cacgtcctgc gactgctcgg caagcaacac gtccgccagc ggctgcaaac cgtgttcgcg
     4081 cgcgatttgc gctttggtgc ggcgtttggg tttgtagggc agatacaggt cttccagcgc
     4141 ggttttgtta tcggcggctt cgatttgcgc cctgaggtcg tctgaaagct tgccttgctc
     4201 ttcaatgctt tttaaaacaa cggctttgcg ctcttccaac tcgcgcagat attgcagccg
     4261 ctcggcaagc cggcgcaact gcgtatcgtc caacccgccc gtcgcttcct tgcggtagcg
     4321 ggcgataaac ggcacggtcg cgccgtcgtc caaaagctcg acggcggcgg tgatttgcgc
     4381 ggcagtcgcg gagagttctt gggaaagaat ttgagtaatg ttcatcaata aaattccaac
     4441 ggacaggccg tctgaaattt cagacggcct gatttaaaaa caatcgcttt aaggcagcga
     4501 attataatat tcgtaggctt tgtccatatc ttcaaactgg tacatatgcc ctttttccag
     4561 caccatcgca ttatcgcaat attgcttcat ggcgctgtgg ctgtgcgaca ccaagatgat
     4621 ggaacggtct ttgcgctttt caaacaactc gtacttacat ttatcggcaa aacgcgagtc
     4681 accaactgca atcacttcgt caatcaaata gcaatcaaac tctaccgcca acgacaaagc
     4741 aaatgccaaa cgcgccttca tacctgaaga ataacgcttg accggctcgt aaagatattg
     4801 ccccaactcg gaaaactctt cggtaaagtt cttcacataa tcgatatcga cattgtaaat
     4861 ccggcagatg aaacgcaaat tgtccatacc ggtcagactg ccttgaaacg caccggagaa
     4921 tgccaaaggc caagaaatac tcattgtccg cttgatttca cccgtggtcg gcggctcaac
     4981 gccactgatc aaacggatga gcgtcgattt acctgcaccg ttgcggccga gaataccgat
     5041 tttctcgccc ttctccattt tgaagctaat atcgtgcaag actgtccgcc aaccttggcg
     5101 ggtcagatag cgtttggaaa cgtgttcaac tgaaatcatt gcggctcaac ccctttgctg
     5161 aatctcgcca ccaccgccaa accaagcagc agcaaaacca agttacacaa caaaatatac
     5221 caaggatttt cgtaagtcgt gacgctgtcg ccaaaatatc cggcacggaa catttctgta
     5281 ccatgcacca tcggaatcat cagcacatag tgttgcaact gctgaggcag gttgtgtacg
     5341 aagaaaaaca cgccggacaa aggcatcatc acaaaactga tggtactcca aactttgcca
     5401 aacggttcaa aatgaaacgc caccgaacag atgaccagcc ccaatccaac ggcaaacata
     5461 gccatcaaaa gccaagccaa caacatatag aaaatatctg ccggtacatc aatccaaccg
     5521 atgataacca atgcgaacat aatcacgacc tgcgcaatgg tggcgccggc aatctccaac
     5581 aacatacgcg caaaaatggt atccaatacg cgtacatttc ggtgatacag caggcttgtg
     5641 ttggcagaaa tcgaaccgat agcatgatta gaagcattac gccacatcat catcatcgga
     5701 tagcctgtca gcgtgaatgc cacgatattc aaagctgaca cgttgtgcat acggaaaaac
     5761 ttccacatca acaccataac caaagtcagc agcaaaggtt cgacaaacaa ccacaaaaaa
     5821 ccaatgttat tacgaccgta gcgagtaata atttcacgca ttaagagtgc gccgattacc
     5881 cttttttgga taagcagcga ctccaaaaac gatgttttat gtagctcttt catcagtttt
     5941 tatgctcacg gatacttgca gacagcaaca cgacaatgcc atagacaatc aaaccgatga
     6001 caaacgttgc cacgatgttg tacaaacggt ttggctcata tgccaagtca ggtttgttcg
     6061 gctgggaaat cacttccaaa tacaattgct gacgatctgc ttccgctttt gcactttcca
     6121 aagaagtcat cgccgcagcc aattgttttt cggccaattc gttttccaaa tacacgcgtt
     6181 gatattccgc cgcctgattg gacaacgaac cctcgccacc gccggaaatc gctttcattt
     6241 gttgcgagat ttctttacgc aaactcttct cacgcgcaat caaacctgga atttgcgggt
     6301 tttcaggcgt aacggctttt acctggtcca gctgggtctg aatgacaatc aactcgtcct
     6361 gtaatttgga taccaaaccc atttgcacat cggattgcgc cttcaagtcg aaaataccgt
     6421 tagacacacg gaattttgtc aattggacag aagcctcttt aacttgctct tcagcagaat
     6481 tgaccacttc ctgagcataa cgaatcgtat cctgacgcgc acgttcgttc agctggttaa
     6541 tcaatacctc accctgttta agcaaagcat cattgatctt ttgagattca ccggcattaa
     6601 acgatgtaac gctcaaattg gaaatgcctg agacagagtc aaaatggatg gataccttat
     6661 cacggtagta ttgataaaac gcctcatcct cgccacgcag gccaaaacca ttaaaacggc
     6721 tgaaaatatc gccttctttt tcataaaaat cgcgaatggg cattttctta cgcaacgcat
     6781 ccaaagccga acgcgactgc atatattcct gcacggtata aatatcgtct tgcgcacggg
     6841 caaaacctgt gccctgcaaa atggcaccca ggccattgag agaagattgg cttttaggcg
     6901 agcgcaccac aaagctcgat tgcgacgtaa aacgatcgga agcgaagaag ccgaaataca
     6961 ccaacgaaat taccgtaggg ataatcaccg ttacccaaaa taaagggctt agctttttaa
     7021 tccaactttt ctttttcggc tttttacgct cggctttggt ttcagtggca actgccacag
     7081 gaagttgctc agacatctta attactcaca ttaattagtt aaattattaa tactgttcgc
     7141 gccactggta accggcgaga acacaaacga caagaatttc tgcacttcag ccaacggcgc
     7201 attcgacaca tacaatacat ctttattctt cacaggaaag cgctgcatag aaaatagcga
     7261 atgcgcatca gccatattca cacgatatac cgttggaatc tctgcctcac tgccataacc
     7321 ttgagcaatc catttatcct gacgttctgc cggcaattcc accaatggcg tatagcggaa
     7381 cacaaacaca ccacgcgcat cagaacggcg atcttgcaaa ccgcccatac ggccaatggc
     7441 ttcagaaagc gataagcctc tggctgaaaa accgatttct tgtgttctcc ccaccgcacc
     7501 catagacgta aaggtatagg gattggtaat catggtaacc acatcaccgc gacgcagcaa
     7561 aatattttgt cgcggatttg caactaaatc ttccaaggca acagttcgta ctacattgcc
     7621 acgtgtcagc tgcacattcg tatcctgcac atttgccgtt gaaccaccta ccgcagccac
     7681 cgcatccaac acacgctcac cggctgccgt caacggcata cgcacactat tgcctgcgcg
     7741 aatcaccgat acatttgccg cattattctg caccaagcgc accatcactt gcggctgatt
     7801 ggccattttt ttcaggcggc ctttaataat ttcctgaacc tgaccaggcg ttttaccgac
     7861 caccgaaata tcgccaacaa acggcacaga aaccgtacca cgtgccgtca ccagttgctc
     7921 cggcaacttg gtttgttgcg cactacccga gcccatcgaa gaaaggccac caccaaacaa
     7981 taccgctggc ggcgcttccc aaatcataat atccaataca tcaccaatat ttagcgtacc
     8041 ggtagaagca taaccatcgc caaactgggt aaaagactga ttcacctgag ctttatacaa
     8101 agactgcgct acagcatgat tgacgtcaat caactctact tcaggaattt gagcttcaga
     8161 ctgttgcccc aatgagacaa ctttttttgc acttgggcca gaggagggaa tagcactaca
     8221 tgcacttccc aaaattaaaa aagaaattac aatacaaaac tttaacttaa gcataaaata
     8281 aaaaatctca ttaagtatga ttgtttttaa ataaatttaa aacctaccag agatacaata
     8341 ccactttatt ttgtagaaca caaacgtgta taatatataa cataaacatc atcttcgaaa
     8401 taatattggg gcttaggaag caaaatcatc aaaaaacgtg ataagctcct aatattttta
     8461 acacattact atattacaca taggatattc caatgaaagt cttaaccgtc tttggcactc
     8521 gccctgaagc tattaaaatg gcgcctgtaa ttctagagtt acaaaaacat aacacaatta
     8581 cttcaaaagt ttgcattact gcacagcatc gtgaaatgct agatcaggtt ttgagcctat
     8641 tcgaaatcaa agctgattat gatttaaata tcatgaaacc caaccagagc ctacaagaaa
     8701 tcacaacaaa tatcatctca agccttaccg atgttcttga agatttcaaa cctgactgcg
     8761 tccttgttca cggagacacc acaacaactt ttgcagctag ccttgctgca ttctatcaaa
     8821 aaatacctgt tggccacatt gaagcaggcc tgagaactta taatttatac tctccttggc
     8881 cagaggaagc aaataggcgt ttaacaagcg ttctaagcca gtggcatttt gcacctactg
     8941 aagattctaa aaataactta ctatctgaat caataccttc tgacaaagtt attgttactg
     9001 gaaatactgt catagatgca ctaatggtat ctctagaaaa actaaaaata actacaatta
     9061 aaaaacaaat ggaacaagct tttccattta ttcaggacaa ctctaaagta attttaatta
     9121 ccgctcatag aagagaaaat catggggaag gtattaaaaa tattggactt tctatcttag
     9181 aattagctaa aaaataccca acattctctt ttgtgattcc gctccattta aatcctaacg
     9241 ttagaaaacc aattcaagat ttattatcct ctgtgcacaa tgttcatctt attgagccac
     9301 aagaatactt accattcgta tatttaatgt ctaaaagcca tataatatta agtgattcag
     9361 gcggcataca agaagaagct ccatccctag gaaaaccagt tcttgtatta agagatacta
     9421 cagaacgtcc tgaagctgta gctgcaggaa ctgtaaaatt agtaggttct gaaactcaaa
     9481 atattattga gagctttaca caactaattg aataccctga atattatgaa aaaatggcta
     9541 atattgaaaa cccttacggg ataggtaatg cctcaaaaat cattgtagaa actttattaa
     9601 agaatagata aaatgtttat acttaataac agaaaatggc gtaaacttaa aagagaccct
     9661 agcgctttct ttcgagatag taaatttaac tttttaagat atttttctgc taaaaaattt
     9721 gcaaagaatt ttaaaaattc atcacatatc cataaaacta atataagtaa agctcaatca
     9781 aatatttctt caaccttaaa acaaaatcgg aaacaagata tgttaattcc tattaatttt
     9841 tttaattttg aatatatagt taaaaaactt aacaatcaaa acgcaatagg tgtatatatt
     9901 cttccttcta atcttactct taagcctgca ttatgtattc tagaatcaca taaagaagac
     9961 tttttaaata aatttcttct tactatttcc tctgaaaatt taaagcttca atacaaattt
    10021 aatggacaaa taaaaaatcc taagtccgta aatgaaattt ggacagattt atttagcatt
    10081 gctcatgttg acatgaaact cagcacagat agaactttaa gttcatctat atctcaattt
    10141 tggttcagat tagagttctg taaagaagat aaggatttta tcttatttcc tacagctaac
    10201 agatattcta gaaaactttg gaagcactct attaaaaata atcaattatt taaagaaggc
    10261 atacgaaact attcagaaat atcttcatta ccctatgaag aagatcataa ttttgatatt
    10321 gatttagtat ttacttgggt caactcagaa gataagaatt ggcaagagtt atataaaaaa
    10381 tataagcccg actttaatag cgatgcaacc agtacatcga gattccttag tagagatgaa
    10441 ttaaaattcg cattacgctc ttgggaaatg aatggatcct tcattcgaaa aatttttatt
    10501 gtctctaatt gtgctccccc agcatggcta gatttaaata accctaaaat tcaatgggta
    10561 tatcacgaag aaattatgcc acaaagtgcc cttcctactt ttagctcaca tgctattgaa
    10621 accagcttgc accatatacc aggaattagt aactatttta tttacagcaa tgacgacttc
    10681 ctattaacta aaccattgaa taaagacaat ttcttctatt cgaatggtat tgcaaagtta
    10741 agattagaag catggggaaa tgttaatggt gaatgtactg aaggagaacc tgactactta
    10801 aatggtgctc gcaatgcgaa cactctctta gaaaaggaat ttaaaaaatt tactactaaa
    10861 ctacatactc actcccctca atccatgaga actgatattt tatttgagat ggaaaaaaaa
    10921 tatccagaag agtttaatag aacactacat aataaattcc gatctttaga tgatattgca
    10981 gtaacgggct atctctatca tcattatgcc ctactctctg gacgagcact acaaagttct
    11041 gacaagacgg aacttgtaca gcaaaatcat gatttcaaaa agaaactaaa taatgtagtg
    11101 accttaacta aagaaaggaa ttttgacaaa cttcctttga gcgtatgtat caacgatggt
    11161 gctgatagtc acttgaatga agaatggaat gttcaagtta ttaagttctt agaaactctt
    11221 ttcccattac catcatcatt tgagaaataa gttaaattat gaagaacctt tgagtgcaat
    11281 tcgaaggttc ttcattcata ttattcatat tttggagaaa ttatgttatc taatttaaaa
    11341 acaggaaata atatcttagg attacctgaa tttgagttga atggctgccg attcttatat
    11401 aaaaaaggta tagaaaaaac aattattact ttttcagcat ttcctcctaa agatattgct
    11461 caaaaatata attatataaa agatttttta agttctaatt atactttttt agcattctta
    11521 gataccaaat atccagaaga tgatgctaga ggcacttatt acattactaa tgagttagat
    11581 aatggatatt tacaaaccat acattgtatt attcaattat tatcgaatac aaatcaagaa
    11641 gatacctacc ttttgggttc aagtaaaggt ggcgttggcg cacttctact cggtcttaca
    11701 tataattatc ctaatataat tattaatgct cctcaagcca aattagcaga ttatatcaaa
    11761 acacgctcga aaaccattct ttcatatatg cttggaacct ctaaaagatt tcaagatatt
    11821 aattacgatt atatcaatga cttcttacta tctaaaatta agacttgcga ctcctcactt
    11881 aaatggaata ttcatataac ttgcggaaaa gatgattcat atcatttaaa tgaattagaa
    11941 attctaaaaa atgaatttaa tataaaagct attacgatta aaaccaaact aatttctggc
    12001 gggcatgata atgaagcaat tgcccactat agagaatact ttaaaaccat aatccaaaat
    12061 atataaaatg cgtaagatta cttttattat ccctataaaa cagtctttaa taaaacctga
    12121 ttgctttata cgcctctttt ttaatttatt tttgctaaaa aaattctcaa gtaaatacgg
    12181 attttctata ttagttgcag acaacagtaa cttcctttgg aaaaatatta ttaaattaat
    12241 tacaaaattt tacaaatgta attatattag tattaaatct cataatactt tttatacgcc
    12301 tgctaaaatt aaaaatgcag ctgccatcta tagttttaat accttgaatt caaattacat
    12361 tttattctta gatgttgacg ttttattatc ggaaaatttt attcaacatt taataaaaaa
    12421 aacaaaaacc aatatcgcct ttgattggta ccctgtttca ttcttaaaca aacaatttgg
    12481 gattataaat tttatattat tctcatataa aggtaatcta aatatagaag aatcattcat
    12541 tatacaaaca gggtttgtaa ctggcttaca attatttaat tctgattttt tctacaaaac
    12601 agctggatac aatgaaagct ttcttggcta tggctgtgaa gatattgaaa tgattcacag
    12661 agcaacatta ttattaaata ttagacctgc ctttaatgaa aatcatcaat attttacaga
    12721 tgatagagga tatatgcctt ctaaattaac cggatttcga aattattttt attatttgaa
    12781 aagagatgaa ttttcaaact tacagataac tcctaaacat ttctggcata agcgaaaaaa
    12841 taaatcaaaa tatctaaaaa atagatatca aaatgatgta aaaatgattc agattatgaa
    12901 agattttgat cgaaaatttc taaaaaatta acgagctgtc ttgcccatat gaatcctgat
    12961 tactttaatt taattatgaa aaatattctc gttaccggcg gcaccggttt tatcggctcg
    13021 cacaccgttg tttctttgct gaaaagcggc catcaagtcg tgattttgga taacctatgc
    13081 aattccagca tcaatatcct gccacgcttg aaaacgatta ccggccaaga gattccgttt
    13141 tatcaaggcg atatccgcga tcgtgagatt ttgcgccgta tttttgcgga aaaccgcatt
    13201 gattcggtga ttcattttgc cggcttgaaa gcggtgggtg aaagtgtggc cgagccgatg
    13261 aaatattatg ataataatgt ttccggcagc ttggtgttgg cggaagaaat ggcgcgtgcg
    13321 ggcgtgttta gcattgtgtt cagttcttcg gcgacggttt atggcgatcc gggcaaagtg
    13381 ccttataccg aggatatgcc actgggcgat accaccagcc cttacggcac atcgaaatca
    13441 atggttgagc gcattctcac tgatattcaa aaagccgatc cgcgctggag cgtgattttg
    13501 ttgcgttatt tcaatccgat tggcgcgcac gaaagtggtt tgattggcga gcagccaaac
    13561 ggcatcccga ataatttgtt gccttatatc tgccaagtgg cggcaggcaa actgccgcaa
    13621 ttggcggtat ttggcgatga ctaccctacc cccgacggca cggggatgcg tgactatatt
    13681 catgtgatgg atttggcaga aggccatgtc gcggctatgc aggcaaaaag taatgtagca
    13741 ggcacgcatt tgctgaactt aggctccggc cgcgcttctt cggtgttgga aatcatccgc
    13801 gcatttgaag cagcttcggg tttgacgatt ccgtatgaag tcaaaccgcg ccgtgccggt
    13861 gatttggcgt gcttctatgc cgacccttcc tatacaaagg cgcaaatcgg ctggcaaacc
    13921 cagcgtgatt taacccaaat gatggaagac tcatggcgct gggtgagtaa taatccgaat
    13981 ggctacgacg attaagttga cctgatacag gccgtctgaa agagatgttt tcagacggcc
    14041 tctttatctg aaaaacacac attctgtctg ctataatctg tttatatttt ttggctatcc
    14101 tctgaaattt atgagaaaaa tccttgttac cggcggtgcg ggctttatcg gttctgccgt
    14161 tgtccgtcat attatcc
//
LOCUS       DAWFKN010000052        14112 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFKN010000052 DAWFKN010000000
VERSION     DAWFKN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.052"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..395)
                     /locus_tag="WGR46_001427"
     CDS             complement(<1..395)
                     /locus_tag="WGR46_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856350.1"
                     /translation="MKITHCKLKKKVQKELIRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDKSDFGGRRKGRRGRGAAGKVVVFGILKHNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYT"
     gene            complement(628..2685)
                     /gene="ppk1"
                     /locus_tag="WGR46_001428"
     CDS             complement(628..2685)
                     /gene="ppk1"
                     /locus_tag="WGR46_001428"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0856351.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2854..3297)
                     /locus_tag="WGR46_001429"
     CDS             complement(2854..3297)
                     /locus_tag="WGR46_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0856352.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3304..3819)
                     /locus_tag="WGR46_001430"
     CDS             complement(3304..3819)
                     /locus_tag="WGR46_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0856353.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4089..6719
                     /locus_tag="WGR46_001431"
     CDS             4089..6719
                     /locus_tag="WGR46_001431"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0856354.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYANFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYSLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLNGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVSVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVFAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNSAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6948..7739
                     /locus_tag="WGR46_001432"
     CDS             6948..7739
                     /locus_tag="WGR46_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0856355.1"
                     /translation="MNLFFDTELGRQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYGFEDKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            7869..7982
                     /locus_tag="WGR46_001433"
     CDS             7869..7982
                     /locus_tag="WGR46_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0856356.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
     gene            8005..8951
                     /locus_tag="WGR46_001434"
                     /pseudo
     CDS             8005..8951
                     /locus_tag="WGR46_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(9334..9645)
                     /locus_tag="WGR46_001435"
     CDS             complement(9334..9645)
                     /locus_tag="WGR46_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0856357.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(9642..10009)
                     /locus_tag="WGR46_001436"
                     /pseudo
     CDS             complement(9642..10009)
                     /locus_tag="WGR46_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10508..10750
                     /locus_tag="WGR46_001437"
     CDS             10508..10750
                     /locus_tag="WGR46_001437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856358.1"
                     /translation="MDGFPLIPYGASVFPASYFPLGIPDLVVVQLYFHVFFAVFSDAV
                     KNQVSSIRADWTGLYGQEGGRQDNNQPEILVLIGIG"
     gene            complement(10810..10895)
                     /locus_tag="WGR46_001438"
     tRNA            complement(10810..10895)
                     /locus_tag="WGR46_001438"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10859..10861),aa:Leu,seq:gag)
     gene            complement(10909..11259)
                     /gene="secG"
                     /locus_tag="WGR46_001439"
     CDS             complement(10909..11259)
                     /gene="secG"
                     /locus_tag="WGR46_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0856359.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(11266..12039)
                     /locus_tag="WGR46_001440"
     CDS             complement(11266..12039)
                     /locus_tag="WGR46_001440"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0856360.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            12223..12726
                     /locus_tag="WGR46_001441"
     CDS             12223..12726
                     /locus_tag="WGR46_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0856361.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            12828..13484
                     /locus_tag="WGR46_001442"
     CDS             12828..13484
                     /locus_tag="WGR46_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0856362.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLASRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            13563..13886
                     /locus_tag="WGR46_001443"
     CDS             13563..13886
                     /locus_tag="WGR46_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0856363.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAVEIDG
                     NMMRY"
BASE COUNT         3172 a         3492 c         3970 g         3478 t
ORIGIN      
        1 tcggtataaa caatgctgtc cggcatgatt ttcttcttga tgacagggag taacgtttca
       61 gacttggcat tatccaccac aacggtatag acccgtccgt tgtgtttcag aatgccgaag
      121 acaaccactt ttcctgccgc accgcgacca cgtctgcctt tacgccgtcc gccgaaatcg
      181 cttttgtccg gctcgacaga gccctcaaaa acctcatcgg cagccaaggc caaatgatgg
      241 ttgataaccg tgcggatttt acggtagaac agtgctgccg aattgggatg gatacccaaa
      301 atatcggcgg cagaacgggc ggtaacttcg agtacaaaaa aacggatcag ttctttctgt
      361 acttttttct ttaatttgca gtgtgttatc ttcatattcc cagggtaaca tatctgctaa
      421 tctagtacag cccctaaaat ttaatccact ataatgccgt ctggaatgcg gcggcttggg
      481 gttgggagcg tataggggaa gtgcttgtgc aggaatggcg aaggtgtgtt tttgactccg
      541 gtccgccctg tttagattgc tgtaaaacta tgccgtctga aacggaaagg tttcagacgg
      601 catggttttt gattggggcg gtgcgggtca gcctccgagc agtgtccaca aatcgttttg
      661 caggtcggct tcggattcgc cctcggcggg cgcggcgcgg atatagccgc cgtcgggctg
      721 catcagccac gcgtgcgtat tgtctgccaa tgccatttcc aaaccttcgc ggatgacgcg
      781 ttttttgagt tcgggcgcgg taatcggcgc ggcggtttcg atgcggcgga agaagttgcg
      841 ccccatccaa tccgcgctgg agataaaggt atcgtccgcg ccgttgttgt ggaagcaata
      901 cacgcgcgcg tgttcgagct gcctgccgat gatggagcgg acgcggatgt tttcggacaa
      961 gccttttaca cccgggcgca aggtgcacat accgcgcaca atcaaatcga tttgtacgcc
     1021 tgccgcgctt gcccgataca gggcttcgat gacggtcggt tcgatgagcg aattcatctt
     1081 ggcggtaatc cgcgccggtt tgccggcttt tgcgtgttcg gtttcgcgtg cgatgcggtc
     1141 gataaccatt ttgtgcaggg taaacgggct ttggtagagt ttgttcagcc gcccgggttt
     1201 gcccaagcct gtgatttcca taaacaatgt gttcacatcg gcggtgattt gttcgtcggc
     1261 ggtaatgatg ccgaagtcgg tgtagatgcg cgatgtgcct tggtggtagt tacccgtgcc
     1321 gaggtgggcg taacgcttga gtacgccgtc ttcgcggcgg atgaccagtg ccattttggc
     1381 gtggactttg tagccgaaca cgccgtacac gacgtgcgcg cccgcctctt cgagctgctt
     1441 cgcccagttg acgttgttgg cttcgtcaaa acgcgccatc agttcgacga cgacggttac
     1501 ttgtttgccg gcgagtgccg ccttcatcag ggcgcggacg agttcggaac gcgtgccggt
     1561 gcggtaaatc gtcattttga cggcaagcac gtcggggtcg gcggcagctt cgcgcatcat
     1621 ttcgacaacg gggtcgaacg attggtaggg gtggtgcagc aggatgggcg attggcgcac
     1681 caaatcgaat atggacgcgg ttttgcccaa ggctttcagt ctgcccggcg tgtgtgtggg
     1741 aaatttcaaa tcggggcggt tgactaggtc ggggacggcg ttgaggcgca cgaggttgac
     1801 cgggcctttg acctgataga gttcggcggc ggtcagtttg aattgcgcga gcagaaagtc
     1861 gcggatgtag gcgggacagg tgtcggcgac ttcgagccgc acgccgtcgc cgtattcgcg
     1921 gtcgtgcagt tcgttttgga tggcggcgcg gaggttttgc aggtcttctt cgtcaacggt
     1981 caagtcgctg tcgcgcgtca ggcggaactg gtggcagcct ttgacgttca tgcccgggaa
     2041 gagtttgccg acgtgggcgt ggaggatgga cgacaggaag acgaagccgt gtccgccgcc
     2101 acacagttcg gacggcaggg gaacgacgcg cggcaggatg cgcggtgcct gcacaatcgc
     2161 catccccgaa ggcctgccga atgcgtccgt gccgtcgagt tcgacggcga agttgagcga
     2221 tttgttcagc ggtcgcggga aggggtggga cgggtcgagt ccgatggggg tcaggatcgg
     2281 cagcagttcg cggtcgaaat agtcttcgat ccattttttc tgcgcgcccg tccagttgcg
     2341 gcggcggtaa aaatggatgc tttcccgcgc caactcgggc tgaaggacgt tgttgaacag
     2401 gtcgtactgg tgccgtatca gggagcgcgc cgcttcggta acgtcggcga tggtttcaga
     2461 cggcattttg ccgttgtcca gcctgcgccg ggggtgcagc ttgttttcac gcttgagcca
     2521 cgccatgcgg acttcgaaaa actcgtcaag attggacgac acgatgcaca ggaagcgcag
     2581 gcgttccaaa agggggacgt ttttgtcttc cgcctgtgcc aacacgcggc ggttgaatgc
     2641 cagcaggctt agttcgcggc agaggatgcg gttttgttcg ggcataaggt tctcccaaag
     2701 atggttgttg ttcggtcgga gcgggcaggc aatgccgtct gaaggcttgg cggcatctgc
     2761 gccggtttca gacggcatga cgtggcggac agcctgtttc aggcatcggg gcaatgtgaa
     2821 aacagatacc ggcgcgtgcg gtatccgtct gtttcaaatc acttccaaca caaaataatg
     2881 acgcagtttt tcgtacaccg catcgctgac gttgatgcag ccgttggtca taatcctgtc
     2941 ggacacggac ggggaggcga tgcgttcgtt ccgcctttcc gacggtatct gattccaaac
     3001 gcggtgcagg gcgaaaagaa aatcaccctc ctgcttgaaa ccgatgactt cgccgccgta
     3061 tccaggcttg tcggtactgt tcagcgtcag cccgaacgtc cccttgggcg tggcaacacc
     3121 gatgaggacg gggtggcact gcctgtcatc ggcaaaacag agttccgctt tggatgtgtt
     3181 tacgatgact tttttctttt gaatataagc gttgaccgca tccggttgcc cctgtgcata
     3241 tacgggggca accattgaca ggccgcataa tatccctaaa aacaggcgga acggcatgtc
     3301 ggattattgg cggatacgtt tcggttttgc cggcacgatt tcgcggatga tgacttgcgg
     3361 ctcggctttg ggtgcgggcg gcggacaggc ggcatctttc gggaagacgg ggttccagta
     3421 gaagctgcgg gcaaatttgt ctttatcgaa aatcactttg tattggcagg tagtaacatc
     3481 ttctacgccg gaagtgtttt cagggtcgat acctacgccc ggggtatgga agtggaacag
     3541 gtaatcccat tcgcgcacgc cgtacatacc ttcgtcgtaa tgcgggcggc ccaggatttt
     3601 gtagatgtcg tctttggtca ggccggggcg catctgatcc agttcgtcat aagtcgggaa
     3661 tgtgccgcgc ttgttgtcga gcgttacgga atagggtttc gggaaaaccg gattgtcggt
     3721 cgtgccgtcg gctttgacgt tgcttttggt tgcgcaggca gacagaacgc ccgccgccaa
     3781 tactgccaag cccggtttga cgatttgttt gattttcata tgcagaatcc tcttttaccc
     3841 gatgcccgtc tgccttttgt tcggaagagc cgcatcagga gagttttaaa catttgattt
     3901 tggtttgtaa tattagcata aaaaacgtgc gtatcaataa acgcggtgta tttgtacggc
     3961 atacggaata atgcgcgtgc gaatttacgc atcctgccgg caatctgccg attcgccgct
     4021 tccggcaacc tgttataatt gatgccgact ttatttttcc aacgttttta agcgaaaaca
     4081 gaatgactat gcaagaacaa tatcaacccg ccgccatcga gcctgcggcg cagaaaaaat
     4141 gggacgacgc ccgtattttc aacgtctccg aagacgcttc caaacccaaa tattattgcc
     4201 tctccatgtt cccctatccc agcggcaagc tgcatatggg gcatgtgcgc aactacacca
     4261 tcggcgatgt attgagccgc ttcaaacttt taaacggctt caacgtcatg cagcctatgg
     4321 gttgggacgc gttcggcatg ccggcggaaa acgcggcgat gaaaaacaac gtcgcccctg
     4381 ccgcttggac gtacgataac atcgaataca tgaaaaccca gctcaaaagc ctgggttttg
     4441 cgattgactg ggcgcgcgaa accgcaacct gcaaacccga atactaccgc tgggaacaat
     4501 ggctgtttac caagctgttt gaaaaaggca tcgtctatcg caaaaacggc acggtaaact
     4561 gggacccggt cgaccaaacc gtccttgcca acgagcaagt tatcgacgga cgcggttggc
     4621 gttcgggcgc gttgatcgaa aaacgcgaaa tcccgatgta ttacttcaaa atcaccgatt
     4681 acgccgaaga gctgctcaac gacttggaca agctggaaca ctggcctgaa caagtcaaaa
     4741 ccatgcagcg caactggatc ggcaaatctc gcggcatgac cgtgcgcttt gctgtttcag
     4801 acgacagcaa acaaggcttg gaaggcgatt acgcgaactt cctgcaagtt tataccaccc
     4861 gtcccgacac gctgatgggc gcgacttatg ttgcagttgc cgccgagcat ccgctggcaa
     4921 ccgcagccgc cgccgacaaa cccgaattgc aggcatttat cgccgaatgc aaagccggtt
     4981 cggttgccga agccgatatg gcgacgatgg agaaaaaagg cgtgccgacc ggccgctacg
     5041 tcgtcaaccc gcttaacggc gacaaactgg aagtgtggat tgccaactat gtattgtggg
     5101 gctacggcga cggcgcggtg atggctgttc cggcgcacga cgaacgcgat ttcgagtttg
     5161 ccaccaaata cagcctgccg aaaaaacagg tcattgccgt cggcgacaac gcatttgacg
     5221 aaaaccaatg gcaagaatgg tacggcgaca aagaaaacgg cgtattggtc aacagcggcg
     5281 acttgaacgg cttggatttt cagacggcat tcgacgccgt cgccgccaag ctgcaaagcc
     5341 aaggcgcggg cgaaccgaaa acccaatacc gcctgcgcga ctggggcatt tcgcgccaac
     5401 gctactgggg ctgcccgatt cccatcgtcc attgcgaaca atgcggcgac gtacccgtcc
     5461 ctgccgacca attgccggtc gttttgcctg aaaacgtcgt acccgacggc atgggttcgc
     5521 cgctggcaaa aatgcccgag ttttacgaaa ctgcctgccc atgctgcggc ggcgcggcaa
     5581 aacgcgaaac cgacaccatg gacaccttca tggaatcgag ctggtatttc ttccgctata
     5641 tgtcgccaaa attttcagac ggcatggtcg atcctgccgc cgcgaaatac tggggcgcgg
     5701 tcgaccaata catcggcggc atcgaacacg cgattttgca cctcttgtac gcgcgtttct
     5761 tcaccaaact gatgcgcgac gaaggcttgg tcagcgtgga cgaaccgttc gaacgcctgc
     5821 tgacgcaagg catggttgtc tgcgaaacct actaccgcga aaacgacaaa ggcggcaaag
     5881 attggatcaa ccccgccgat gtcgagctga cgttcgacga caaaggccgc cctgttttcg
     5941 ccgtcctcaa agccgacgga ctgcccgtcg tcatcagcgg cacggaaaaa atgtccaaat
     6001 ccaaaaacaa cggcgtcgat ccgcaagagc tgattaacgc ctacggcgcg gacaccgccc
     6061 gtctgttcat gatgttcgcc gcaccgcccg aacagtccct cgaatggagc gacagcggcg
     6121 tcgaaggcgc gcaccgcttc ctgcgccgcc tgtggcgcac cgtttacgaa tacctgaaac
     6181 aaggcggcgc agtcaaagcg ttcgcaggca gccaagacgg tttgtctaaa gaactcaaag
     6241 acctgcgcca caaactgcat tccactatcg ccaaagtcag cgacgactac ggccgccgcc
     6301 agcagttcaa caccgccatc gccgccgtga tggaactgct caaccaatac gacaaaaccg
     6361 acaccggcag cgaacaaggc cgcgccgtcg cccaagaagt attggaaacc gccgtacgcc
     6421 tgctgtggcc catcgtgccg cacatctgcg aaaccctgtg gagcgaattg aacagcgcga
     6481 aactgtggga agcaggctgg ccgacagtcg atgaagccgc cttggtcaaa tccgaaatcg
     6541 aagtcatggt tcaagtcaac ggcaaactgc gcggaaaaat caccgtcgcc gccgacgcat
     6601 ccaaagccga cctcgaagcc gccgcactcg ccaccgaagg cgcagtgaaa ttcatggaag
     6661 gcaagcccgc gaagaaaatc atcgtcgttc ccggacgctt ggtaaatatc gtcgtctaag
     6721 ccgcttttaa ggtttagcca tacggataaa ggccgtctga aacttggaaa cagggtttca
     6781 gacggccttt ttaaggcaga ttgggttgtc cgcatgacag acctcaaata tagtggatta
     6841 actttaaacc ggtacggcgt tgccccgcct tgccctgctg tctgcggctt cgtcgccttg
     6901 tcctgatttt tgttaatcca ctaaaactta atctcaaaat ccaaaatatg aatttatttt
     6961 tcgataccga attgggaagg caacaaaata aagcaaccca caaaatccgt gtaatgagcg
     7021 aggcttggct ggaaaaaaac ggctactgcc cctgttgcgg aagcaagccg atgaagagat
     7081 ttgccaataa caaacctgtt gcagacctct tttgcccaaa ttgccacgag caatatgaat
     7141 taaagagtaa aaatcaaaaa accataggca acagcgtgcc tgacggtgca tatcacacca
     7201 tgttggagcg catccggtca gataccaacc ccaacttttt ctttcttgca tataaaaaag
     7261 cggattactc catacggcaa ttggtgcttg tacccaaaca tttcatcacg ccggatatga
     7321 ttattcccag aaataaaggc attaaaaacc ggccgaacca cattatgtgt tccatcaatc
     7381 tcgccccttt gcctgaaagc ggcaaaatat tcttaataga cgattcccgc attatcgaac
     7441 ccgaaaccgt tctgaaaaaa tggcaatcca acctgttttt acgcaaccaa aatgcggagc
     7501 gcaaaggctg gcttttggct gttatgaaat gtatcgacca actccccgaa gaattcacat
     7561 tgtcgcaaat gtatggattt gaagacaaac tatccatcca atttccccaa aacaaccata
     7621 tcagagacaa aatccgccaa cagttgcaaa ttttgcgcga ccaaaatacg atcgaattca
     7681 ttggtcgcgg actttacaaa aaaatcgaca aattgccccc gacttccaag gcgttttaat
     7741 ttcaaatcat gatactgaat tcacaaggga tatttattct tctgctattc tgaaaacagt
     7801 tgaagttcag cgcaatattg ccgcaaaagg cagcagccgt aaaaaagtcg gggaattatt
     7861 ggcaatttat gcgtgattcc gacaaaccgg ccgcctccga aggcgcagtg aaattcatag
     7921 aaggtaatcc tgccaagaaa atcatcgccg taccgggcag actggcgagc atcgtggtgt
     7981 aaatcttttt aaaaacatca aacaatgtta agatgccgtc cgaaatccgt tttagattca
     8041 gacggcattt tttaaattta attttttaag ggataagcct aatatgaaat ttccttcctt
     8101 ccttccttcc ttccttcctt ccttccttta aacggatagt ctgtctgtcg gcagcaatct
     8161 cgatattggg aggttgtgcg gtcgttgctg atgtttacgg tcatgattcc gccacaatga
     8221 atgctgcggc tgccgaagat tatatgaaaa cggttgagtt gaacaagtct gccggcaatg
     8281 tcgataccac atccagaaca gcccgcaggg tgcaggcagt atttcgacgt atgctgcctt
     8341 atgccgatgc ggcaaataat accggccata agtttgactg gaaaatgacg gttttcaaaa
     8401 acgatgagct gaacgcgtgg gcaatgcccg gtggaaaaat ggcgttttat acggggatag
     8461 tcgacaaact caagctgacc gatgacgaaa ttgccgccat tatggggcat gaaatgacgc
     8521 acgccctgca tgaacacggt aaaaataagg tcgggcaaca aattttgacc aatacggcgg
     8581 cgcagatagg cacgcagatt atattagaca aaaaaccgga cactaatccg gaattggtcg
     8641 gattgggtat ggatattttg ggaacgtacg gcattacctt tccttatagt cgcagcttgg
     8701 aagaagaagc cgatgagggg ggaatgatgt tgatggcgca ggcaggctat catccggcgg
     8761 ccgctgtcag ggtttgggaa aaaatgaatc aggaaaacga ccaaaacggc tttatttatg
     8821 ctattacctc tactcatccg acaaacaatg cccgtataga aaatctaaaa cggttgttgc
     8881 cgaccgttat gccggtttat gagcaaagtg tcagaaataa ggggcgcgtt aataaaaaac
     8941 gtcggcgtta agggaagggg atcgagaatt tgagccgttg tttcaaaatg ccgtctgaaa
     9001 tcgtttgaga ttcagacggc attgaaacag ttctgagccc cgcccgtttt gccttcaagc
     9061 ccgcgccgct taaatccaat cccccctgca acgggcgcaa aaaaatcccc gtgccttgcg
     9121 accggggatg tttcgggttt cgcgcccgtc tgcgccgtgc cttgctgtcg ggctatgtcg
     9181 tctgcaccgc cgccgtttcg gcttggtttg atgcggtcgg gcatttatgc ggctatgccc
     9241 tgatattcgg ttcggcggtc ttgcctgtct tggcaggcat attcagcagc gcgtctattg
     9301 tgaacttgct gattttgccg tttgccaagt cggctacgca atggcttcga tacccttgtt
     9361 tttgacgatg gtcagcagct tcaacgccgc gccgctcggc tttttaacgc cccgctccca
     9421 agccgaaacg tgattttttc ccacgttgag atagatggcg aaagcggctt gcgatagtgc
     9481 ctccttctcc ctgattgcct tgatgtctcc gccgctcaac ggtttgattt cggtcaggca
     9541 ggacttgtca aagccgcgca tagtcttttt gtcgattgcg ccgatgttgt aagccccttc
     9601 catcatttca tgaatggcgg ctaatgcctc gcttttgtat ttcatgattc tatctccgtg
     9661 ataatgccgt cttctttcaa agccgccagc tctgcccgcg tgtatttcag ataatatgcg
     9721 gcggcttttc ggtaaacgtc cagttcttta tccgaaatgt tttccctgtc gttcttggca
     9781 aaggcgtaaa cgaaaatgcc ctatctgcct gtttgaacaa tatcaggctg cgatagccgc
     9841 cgcttctgcc ttgtccttgc cttgctatgc gctgcttaat cacaccgccg cctaaatctg
     9901 cgtctatcag cccgttatcc gcccgctcta ccgcttccag cagctcggaa tcgttgattt
     9961 tgtgcttctt ggcaaatttc actatccatt ggtttttgaa tatccgcatt gtgtagccgt
    10021 tgattagtga tacttggaat tataccagag acagacaaca cggcaagctg ccttttttat
    10081 gccccgcaat ttctccgttt atgccgcctg aaacactggc ggctaacctg attatttttt
    10141 aatcaatcgc ttttaatggc tcaaattgcg tttttagcgc gtttcgctgt cttcctatgc
    10201 ccccatatat aaccaagtgg tttaaccgcc attcctgctc aaaataggcg cggttccgtg
    10261 cgttttggct tttcattccg ctacgctccc actgccaaga cggtgaaaaa ccggaaaacc
    10321 ccaaaagttg ttaccgattc gttaccattt gttaccgcat ttgttaccaa ttttttatct
    10381 ttttcttatt ttatagtgaa ttaaatttaa accggtacgg caaggcgagg caacgccgta
    10441 ccagtttaaa tttaatccac tatagtatag gttttttcca aaaacccaaa aaaaaccgct
    10501 ctgcatcttg gacggtttcc ccctcattcc ctacggtgcg tcagtattcc ccgcaagcta
    10561 tttcccgctt ggcattcctg atttggtggt cgttcagctt tattttcacg tatttttcgc
    10621 ggtattttcg gatgccgtaa aaaatcaggt tagttcaatt agggcggatt ggacgggatt
    10681 gtacggtcag gaagggggac ggcaggataa caatcagcct gaaatccttg tattgattgg
    10741 gattggttga cggcattaga caggattaaa cgaaaaaaaa cgcttagaat ttctaaacgt
    10801 tttgtgtgtt ggtgccgaca gcgagatttg aactcgcaca gcctacggcc actaccccct
    10861 caagatagcg tgtctaccaa tttcaccatg tcggcatttg aaaaactgtt atttctgctg
    10921 ctgaggaaca ggggcagaag gttcggtatt gcttacgggt ttgggtgctt gctgagtttg
    10981 ttgtacgttg ctgaagtcca aaccgtgttt tgtcgtgtgg gtgtgaatat acaccatagc
    11041 catgcaggtt gcaaagaaaa atgttgctgc aacggcggtc gaacggctga ggaagttagc
    11101 gttgccggca gagccgaata cgccttgcgc gctgccgctt cccgatccga aggtcgcgcc
    11161 ggcatccgcg cctttgccgt gttggagcaa tactaacacg atgacggcca aagcggaaat
    11221 tatattaaca atccaaatta gggttttgaa ggcttccata tttttctacg cattttgtgc
    11281 ggcactgatg atggcggtaa aggagtcgta cgacaatgac gcgccgccga cgagtgcgcc
    11341 gtccacataa ggtactgcga agatgtcggc cgcgttgtcc gctttcacac ttccgccgta
    11401 aaggacgcgg attttaacat cgcttccgca caaagacaag atttctttgt agatgaatgc
    11461 gtgcatatcg gcaatctgtt cgacggtggc gactttgccg gtgccgattg cccagacggg
    11521 ttcgtaggcg acggcgatgt ttttggtgtc cagtccttgg aggatggaaa gctgatgggc
    11581 gatgacttcg tgttctttgc cggcttcgcg ctcttcgagg ctttcgccga cgcacaataa
    11641 cgggatgagt ccgacgttga ggacgttttc cattttgcgg cgttggattt cgtttttttc
    11701 gccgaaataa aggctgcgtt cggagtgtcc gatgaggacg atgtctgtgc cggtgtcggc
    11761 gagcatttcg gcggacactt cgccggtgta cgcgccgttg tcggggaagc ggctcacgtc
    11821 ttgggcgcag gtgaggatgc ggttgtttaa gacgatttgc atggcgttgt gcagttgcag
    11881 caggtaaacg gtcggggcgg cgagtccgat gaggacgcgt tcggcggtgg ggaggatgcg
    11941 gaagcggtgc atgagcgaat tgttgttctg gagccggccg ttcattttcc agttgccgat
    12001 gacccatttt tgatcccaca ttccgatttg gtgatacatc ttttttgctc cgtgtcgtgt
    12061 tttttctgtc tgccgcgtgt ggcgcgttgc aatgtgaagt ttagtggata tgcggcgggt
    12121 tcgcaacttg gggcgggcgg ctgcgggggc ggtttggaat gttgtttcgg gcaggttgtt
    12181 ttataatggc cgcctgatat gtatgcaact ataggagatg tgatgcacgc gcttcatttt
    12241 tcggcttcgg acaaggccgc gctttatcgg gaggtgttgc cgcagattga gtctgtggtg
    12301 gcggacgaga cggattgggt ggcgaatttg gcgaacacgg cggcggtttt gaaggaggcg
    12361 ttcggttggt tttgggtggg tttttatttg gtcgatacgc gttcggacga attggttttg
    12421 gcaccgtttc aggggccttt ggcgtgtacg cggattccgt tcggtcgcgg ggtgtgcggt
    12481 caggcttggg cgaagggcgg gacggtggtc gtcggggatg tggacgcgca tcccgaccat
    12541 attgcctgtt cgtctttgtc gcgttcggag attgtcgtgc cgctgttttc agacggccgc
    12601 tgtatcggcg tgttggacgc ggacagcgaa catttggcgc agtttgatga gacagatgct
    12661 ttgtatttgg gcgaactggc gaagattttg gagaagcggt ttgaggcttc gcgccaggcg
    12721 gcttgagtga tggaaagggg cgggcggcgc gtgccgaagt tggcgcggcg ggagtgtggt
    12781 tttataatgc ctgccattgt tgaaacaatt atttgacgga gcactaaatg gattttgaaa
    12841 aagcgcggtt caatatggtc gaacagcaaa tccgtccgtg ggatgtattg gattttgacg
    12901 ttttggacgc tttggcggag attccgcgcg agctttttgt cgatgaggat ttgcagggtt
    12961 tggcgtatgc ggatatggcg ctgccgcttg ccaacggtca taagatgctg gagccgaaag
    13021 tcgtggcgcg gctggcgcag ggcttgaagc tgacgaaaaa cgatacggtt ttggaaatcg
    13081 gcacgggttc gggctatgcg accgcgcttt tggcaaaatt ggctagtcgt gtggtgtcgg
    13141 acgacatcga tgccgaacag caaaaccgcg ccaaagcggt gttggacggc ttgggtttgg
    13201 acaatatcga ttatgtgcaa aacaacgggt tgaccgaact ttccgcaggt gcgccgtttg
    13261 atgcggttta tgtcggcggt gcggtaaacc ttgtgcctga agtgttgaaa gaacagttga
    13321 aagatggcgg acgcatggcg gttatcgtag gccgcaagcc tgttcagcgc gcgcttttga
    13381 ttacgcgcaa gggcgatgtg tttgaagaga aggtgctgtt cgataccttg gtggcgcatt
    13441 tggatgataa ggatgccgat ccgtttggcg gtttcgattt ttaagatgtt gacaggtcat
    13501 gccgtctgaa agcgggtttg gggcttcaga cggcattttg cttggttttt ttggggggtg
    13561 cgatggatat tgtgcaactt gggcccgcgg aattgaaagc gtggatggac gaagggcgga
    13621 tgttttgttt gttggacgtg cgtacggatg aagaaacggc ggtttgttcg ctgccaaatg
    13681 cgctgcatat cccgatgaat ctgattccgc tgcggcagaa cgagctgccg gatgatgtgc
    13741 cgcttgtggt gtattgccat cacggtatcc gcagtctgca tacggcgatg tatttggcgg
    13801 acgcgggttt tgaaaacctg tacaaccttc agggcggcat cgacgcgtgg gcggttgaga
    13861 ttgacggcaa catgatgcgt tattgaggca ggcatttcag acggcattcc ttaaatgcgt
    13921 gtacctgtgg tgttccaaaa ggtcgtctga aagtgcagct tctgcgaagc taaagcgcgg
    13981 tatcaacgaa gttaaaattt gcctgaacct taaaggcagc ctgcacccca atcccctctc
    14041 ccgtgggaga gggctaggac gaggctggca aactgaaagt ttgcttgggc ggcattttca
    14101 atgtgcaggc tg
//
LOCUS       DAWFKN010000053        13677 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFKN010000053 DAWFKN010000000
VERSION     DAWFKN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.053"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGR46_001444"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGR46_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0856364.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(866..1174)
                     /locus_tag="WGR46_001445"
     CDS             complement(866..1174)
                     /locus_tag="WGR46_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856365.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(1158..1460)
                     /locus_tag="WGR46_001446"
     CDS             complement(1158..1460)
                     /locus_tag="WGR46_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0856366.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(1534..3897)
                     /locus_tag="WGR46_001447"
     CDS             complement(1534..3897)
                     /locus_tag="WGR46_001447"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0856367.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAGTAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPQDAPVGVNIREYLDLDDTVFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQSVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSAAQFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTEHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAVNKLVQEIGVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3933..4451)
                     /locus_tag="WGR46_001448"
     CDS             complement(3933..4451)
                     /locus_tag="WGR46_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856368.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIVP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(4458..5522)
                     /locus_tag="WGR46_001449"
     CDS             complement(4458..5522)
                     /locus_tag="WGR46_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0856369.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(5699..6691)
                     /gene="pheS"
                     /locus_tag="WGR46_001450"
     CDS             complement(5699..6691)
                     /gene="pheS"
                     /locus_tag="WGR46_001450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0856370.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(7037..7396)
                     /gene="rplT"
                     /locus_tag="WGR46_001451"
     CDS             complement(7037..7396)
                     /gene="rplT"
                     /locus_tag="WGR46_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0856371.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(7409..7606)
                     /gene="rpmI"
                     /locus_tag="WGR46_001452"
     CDS             complement(7409..7606)
                     /gene="rpmI"
                     /locus_tag="WGR46_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0856372.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(7753..8274)
                     /gene="infC"
                     /locus_tag="WGR46_001453"
     CDS             complement(7753..8274)
                     /gene="infC"
                     /locus_tag="WGR46_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0856373.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(8292..10205)
                     /gene="thrS"
                     /locus_tag="WGR46_001454"
     CDS             complement(8292..10205)
                     /gene="thrS"
                     /locus_tag="WGR46_001454"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0856374.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(10258..10334)
                     /locus_tag="WGR46_001455"
     tRNA            complement(10258..10334)
                     /locus_tag="WGR46_001455"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10298..10300),aa:Val,seq:gac)
     gene            10513..11628
                     /gene="tgt"
                     /locus_tag="WGR46_001456"
     CDS             10513..11628
                     /gene="tgt"
                     /locus_tag="WGR46_001456"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0856375.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            11638..12720
                     /locus_tag="WGR46_001457"
     CDS             11638..12720
                     /locus_tag="WGR46_001457"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0856376.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12911..13379
                     /locus_tag="WGR46_001458"
                     /pseudo
     CDS             12911..13379
                     /locus_tag="WGR46_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(13376..13666)
                     /locus_tag="WGR46_001459"
     CDS             complement(13376..13666)
                     /locus_tag="WGR46_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856377.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3516 a         3654 c         2991 g         3516 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cgcaatatag
      661 tggattaaca aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta
      721 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc agcttcgtcg
      781 ccttgtcctg atttttgtta atccactata attatttttt agcgtgtaaa acaaaaccgg
      841 ctgcatacct gcaaccggcc tcaaatcagc acaattcctt atccaaatcc gccaacagct
      901 ctttcagacc gtctccgatt tcctgaagcg taaccggacg gttgccgtcc gaacccgact
      961 ccgcaccttc tgcatacggt gcaaacgtgc tctgcaattt caacagtttc accacatcca
     1021 aacgggtgta gcgttcgccg ccgtaaccga caaccacacc gtaatcatgc tgccattgcg
     1081 caaaaccata ggggctgatt tgcaacagtc cgcacaactc gtccagcgtg aaatagcgtt
     1141 ttgcgggaac cttcaaatca acgttgtttg tcatagtagt gttccaccat gcttttgagt
     1201 ttctggctgg catggaaagt taccacgcga cgggcggtaa tcggcacttc ctcgccggtt
     1261 ttcggattgc ggcccgggcg ttgcggcttg tcgcgcaact ggaaatttcc gaaaccggaa
     1321 attttgattt cttcgccgct tgccaaagtg ctgcggattt cttcaaaaaa gagttcgacg
     1381 atttctttgg catcgttttt ggtgacgttg ctgactttat ccaccaaaat atcggccagt
     1441 tctgctttag ttagtgtcat atgattacct tctgttgtaa tagttgtgga ttattaccaa
     1501 tttttattta aaattcaagc gaatatttat tttttaactg cgaagccgcg cccctgccgc
     1561 cgttgccgcg ccaatcagtt ttccgataag cggctcgacc gcctcatccg tcagcgtgtt
     1621 ttccatatcc tgcaaaatca ctttgaccgc cacgctcttc atcccttcgg gcagacccgt
     1681 gccgcgatac acgtcaaaca cgccgatttc ctgtaccaac ttgtttaccg cgcctttcaa
     1741 gacaagcagc aaatcatcat ggctcatagc ttccggcatc acaaacgcca aatcgcggcg
     1801 caccggctgg aatttcgata cggcccgata gcgcgttttc ccgcattcca acacggccgc
     1861 catatcgatt tcaaatacca gcggcgcttg cggcaggtcg tatttttgca gccatttcgg
     1921 atgcagttcg ccgacaaagc cgatgacttt gccgtctgaa acgatattgg cggcgcgtcc
     1981 ggggtgcagg gcgggatgtt cggttttgac aaactcgact gctttgtttt tcaacagatt
     2041 ttccacatcc gccttgatgt cgtaaaaatc cgcattacgc gttttctcgc cccattgctc
     2101 gggcatcgcc gcaccgtacc acaaaccgcc gatacgttcg ttttgaacaa attgagcagc
     2161 cgaacccttg ccgaacacgc gggcgatttc aaacacgcgc acgcggtttt gcttgcggtt
     2221 caagttgttt tgcagaattt ccaccaagcc gccgatgagc gtggaacgca tcacggcata
     2281 ctgcgccgcc agcgggtttt gcaggcggat ggggtcggcg ttggcggcaa aatcttgttc
     2341 ccactgctcg tcaacgaagg catagctgac cacttcgcgg tagccgcgcg ccgccatttc
     2401 gttgtaaacg gcaaaacgcg gacggcgtgt ttcgggcagt tccagcattt tcagacggcc
     2461 tgacgtgtaa tcgtcgggga tgttttcata gccgtaaacg cgtccgattt cttcaatcaa
     2521 atcagcctca atttcgatgt cgaaacggaa gctcggcgcg gtaacgcgga agccttccgc
     2581 cgttttctcg ggctgcaaac ccaagtgttg caaaatggtt tccacctgtt cggcagggat
     2641 gtccacaccc aacacggttt tcagacggcc caaacgcaat ccgacctgct tcgcttcagg
     2701 caattcgcct tgcgcttcca ccatttctcc tgccgcaccg ccgcagattt gcaacaccaa
     2761 ttcggtggcg cgttcaatgg catccgcctg caaacggtaa tccacgccgc gctcaaagcg
     2821 gaacgaagaa tccgaaccga aaccgtactg gcgcgatttg ccggcgatga tttcgggcgc
     2881 aaaccaagcc gcttccagca cgatattttg cgtgccgtct gaaaccgcgc tcgccgcgcc
     2941 gcccattaag cccgccaaac tcaacacgcc tttttcgtcc gccacgacca gcgtattttc
     3001 agacagggaa acggttttct cgttcaggca ttccagcgtt tccccttcgc gcgcgcggcg
     3061 gatgtgcagg ctgccggaaa gtttgtcggc atcgaaaacg tgcatcggct ggccgatttc
     3121 cagcattaca taattgccga tgtccaccag cacggaaata ctgcggatgc cgctgcgctc
     3181 caaacgttgt ttcatccaat cgggagtagc cgctttcgca ttgacatttt caataacgcg
     3241 gctgataaaa cggccgcaat cggcaggcgc gttaatctgc acggactgtt ttcgactgcc
     3301 cgtgatcggc gcggtatgga tttcgggctg cctgaacgcg caccccgtca atgcggacac
     3361 ttcgcgcgca atgcctttga tgctcaggca gtcggcacgg ttcggcgtaa ttttcagcgt
     3421 aaacaccgtg tcgtccaaat ccaaatattc gcgaatgttt acaccgacag gagcatcttg
     3481 cggcagaatg tgcaggccgt tcacaccgtc gtcgggcaga ccgagttcgt cggtggaaca
     3541 cagcatcccg ttcgacacca cgccgcgcat cttggtcggc ttgattttga aattgcccgg
     3601 caacaccgcg cccggcaacg aacacggcac tttgatgccc gctttcacat tcggcgcacc
     3661 gcacacaatc tgaaccaact cacccgtacc cgcatcgact cgggtaacgt tcaaacggtc
     3721 tgcatcagga tgtttttcaa cagattttac ttcggcaaca accacgcccg caaaggcagg
     3781 cgcggcagtc ccggcttctt ccacttccaa gccggacatc gttaacaggt gttccagctt
     3841 atcggcggaa agttcggtat cggcttgggt tttcagccat gagtaggaga attgcatgtt
     3901 atgttctcaa ttaactaatt aactttagat tttcaagagc tagctaaacc tttttgaatg
     3961 gctaagtccc tattaattac ttctacagaa atgttccata aagaaatcaa ccgcaaagta
     4021 tctttcttat taaattcttg ctttattaat tcattcaatt gcccaagata attaagcggc
     4081 acatctggga taaccagtaa taatttgatt tctatgctac cccattgcaa atttgaaaaa
     4141 gcactaggca caattgtttc tttgatatct tctctgccta atattcccaa aatttcaaca
     4201 tttaatgcat tacaccattt ttgataaatc tctgaaatat attcatcaaa acgctcagga
     4261 gatctttctt gatttggaat actttgtttt gcctcaacaa ataacaaatt atcttcttgc
     4321 agatagataa actctactgt cttaatacct tcttgccttc tcttccggat atatggttct
     4381 atataaaaca ggttattttt tactagatta aaagttatac ctgattcagt gagatttatt
     4441 atttcactca taaaaaccta accaatccat gctgtttccg gagataaaac acctatttct
     4501 tctctgaaca atcgaataga ctcaccaata atttcattat ccggcatgct attttgtaaa
     4561 ttttcctgca cccaagtagt ctgttccttt tctttgccag gcataaaaac aggaatactt
     4621 tcactatgct ttctagctaa taaacacagc ttcttgataa caaaatatga atgcgtactc
     4681 ataaaaatct gtattcccaa tttggattta gataaggaaa acagaatttc tagaaattga
     4741 ttaattgctt tcggatgtag attagcttct ggctcatcaa taaataagat tgatccagga
     4801 ctcaaaaacc gatttcctaa tagagtgtct aatatcccaa tcttttttac cccctctgct
     4861 gtagaattga tagagaaagt ttgtctatct ttttcatata cccatgcctt ctttttatca
     4921 ttgtagctaa cataaccgcc aaacaatgct tctagatcct ttctagcttc cgctgcctca
     4981 gggtaattcc tcccttttgt tggtttaata tccaatgctt ttaccaaatc aatgtaagta
     5041 gcatcaaaac caaatctctt ttcttcctct gattttttaa ttacatcaaa tagtgataga
     5101 acttctttgg gtggtaaaaa aatagagtta gcgtttattc tgggagaaag ttcgtatagc
     5161 tccggtttaa ttagcttttt ggtagttcta ccaaaagaaa acaaaactga agaactatct
     5221 tctaaagtca tacttaactt aagaggccgt tcgtttggat tttcttttcc tcttgtaact
     5281 aaatcaccaa tttcctctac ttggaacgtc caatatagct tatctcgcaa ttcctcagaa
     5341 aaatcacgaa tattctttcc tttatgagct tcctcatgac tttttaaaac actatataat
     5401 gcctttaaaa taaaagtttt ccctttgcta ttttctgcaa taattaaatt tattttccct
     5461 aaattatcag ccttcaataa gctaatcggc ccgaaatttt caagccaaaa tgatttaatc
     5521 atataatccc cctaatatat atcaacaata tttgtacaag acacattgtg aaaagacagc
     5581 aaccacagca ccatagtaaa cgcatagcag gcggataatg gagcgacata gcgtttcatg
     5641 atttagcttt ctgaggtagg cacaggctac ttttttcatt tttaggcagc ctacacaatc
     5701 attttacaaa ctgcttcaaa aaattcaaat cgttatcaaa aaacaagcgc aaatcgttca
     5761 cgttgtaacg cagcatcgcg aaacggtcga gaccgatacc gaaagcgaaa ccggtgtatt
     5821 tttcgggatc gatattcaca tttctcaaca cgttgggatg caccatgccg cagccgccga
     5881 cttccagcca tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg
     5941 ggaagaaaga cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa
     6001 aatccgtgaa cactgctttc aaatcggcaa aagttacgcc ctcttctacc cacaaacctt
     6061 ccgcctgatg gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg
     6121 gggcgataat gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg
     6181 aagtgtgcgt acgcaaaaca tcgccgtttt caacgtaaaa cgtatcctgc atcgcacggg
     6241 caggatggtt tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag
     6301 gcccgtccgc cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca
     6361 gggttacggg atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atatcgaggg
     6421 cttcggcggc aagtcgggct tgcagcttga cttcgttgag ggcttcgcgt ttggcattaa
     6481 aagccgtctg aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt
     6541 caggcgacat ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttaccaagat
     6601 aacgggcttt gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc
     6661 cttctgcaac gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatac
     6721 cacctgaaac aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta
     6781 ttttcttcac ggtcaatcca ttggataaat tttctgtata gtggattaaa tttaaaccag
     6841 tacggcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
     6901 tgatttaaat ttaatccact atattttaac gcaaatttat ttaaaaaaat agactttaaa
     6961 gaaagaaaat caatcacata gaatttctgc aaacaaagaa aaaaggaagc cgcagcttcc
     7021 ttttcaattt tttggattaa gcagccaaag cagctttggc tttttcaacc aattgtgcaa
     7081 aagcggcttt atcgaacacg gccaaatcag ccaatacttt gcggtcgatt tcaatagagg
     7141 cgcgtttcag accgttcata aatttgctgt aagacaaccc gttttcacgc gcacctgcat
     7201 tgatacggac aatccacaat tgacggaatt ggcgtttgcg ttggcggcgg tcacggtacg
     7261 cgtattgacc ggctttcatt accgcctgct tggcaacgcg gtaaacgttt ttacgacggc
     7321 cgcggtagcc tttggctaac gcgaagattt tttggtgacg ggcacgagcg gtaacaccgc
     7381 gttttacgcg tggcatattc taaactcctt aagcgtaggg taacatttta gcaacagaag
     7441 ccaaatcgcg atcatttacc atagaggtac cgcgcagttg gcgtttgttt ttggtggtct
     7501 ttttagtcaa gatgtggcgt ttgaacgcat gagcgcgttt cacaccgccg ttacccagta
     7561 ctttaaagcg ttttttcgcg ctagacttgg ttttcatttt aggcatggga aaactccatt
     7621 cgttatcgga taaggcatta gggggtttta aaacatcagt ttcaaacact tgaacccaca
     7681 ataccacggt tttttgccgc aattgaaaac ttactccgaa gcggcaatcg gagtaagcgg
     7741 agaattatag ctttattttt tcttcggcgc aatcatcatc accatttggc gaccttccat
     7801 tttgggaaag gactcgattt gcgccacttc agccaaatct tcttttacac gttccaaaag
     7861 ttgcgcgccg agttgctggt gagccatttc acggccgcgg aaacgcaatg tcactttgac
     7921 tttatcgccg tcggcaagga agcggttaat gttgcgcatc ttgatttgat aatcgccctc
     7981 atccgtaccc ggacggaatt tgatttcctt gatttgcacc tgcttttggt ttttcttggc
     8041 ttcgtcgcgt ttcttggcct gctggtattt gtatttaccg taatccatca gtttgcacac
     8101 aggcggttta gcagttgggg aaatctctac caaatcgaca tcctgccctt cggccatagc
     8161 caaagcttca cgaactgaaa cgacaccaag ctgttcgcct gactcactga ttaaacgcac
     8221 ttctttggcg gtaatttcgc cgttgattcg tgcttcgcgt tcttgagcga tgatgaatac
     8281 tcctataaaa attaatgatt gacgagggca tcagtgattt cttgctgcaa ttgcgcaatg
     8341 aaatcatcca aatccaaaga acccaaatct tctgctttgc ggcgtaccgc cactttgttt
     8401 tcctgcttct ccttatcgcc gacaacgatt tgataaggga aacggtattg gctgttgtcg
     8461 cggattttgt aaccgatttt ttcgttacgc aaatccaact cggcgcggaa tcctgccgcc
     8521 tgcaatttgg cagccacttc ccgacaataa tctgcctgat tttcggtaat attcataatt
     8581 accaattgaa ccggagccaa ccataacggg aatgagcctg catggttctc aatcagaatg
     8641 ccgataaacc gctccaaaga acctaaaatg gcgcgatgca acataacagg acgcgcacgg
     8701 tcgttgtttt cagttacata ttcggcattc aaacgctccg gcaatacgaa atccagttgt
     8761 aatgtaccgc attgccaaga acgacccaag gcatctttga catgatattc gattttaggc
     8821 ccgtaaaacg caccctcgcc cggcaattcg ccccattcca cgccgcaggc agtcaatgcc
     8881 tcgcgcaaac cctgctctgc cttatcccac acgtcatctg aacctgcacg tttttcaggg
     8941 cgaagagaaa gcttgacgga tacatcatgg aaaccgaact gtttgtagat gcgaaccaac
     9001 aattcattga acgcacgagc ctcgctgacg atttgatctt cggtacagaa aatatgcgca
     9061 tcatcctgca caaaaccgcg aacccgcatc agaccgtgca gcgcaccgct cggctcattg
     9121 cggtggcaag aaccgaattc cgccaaacgc atcggcaaat ctcgatacga acgcaaaccg
     9181 ttgttaaaaa tttgaacatg acccggacag ttcatcggtt taaccgcata ttcgcgtttt
     9241 tccgaactgg ttacgaacat attgtctttg tagttgtccc aatggccgga tttttcccaa
     9301 aaggttttat ccatgatttg aggcgttttg acctctttat aaccggcggc attcagctct
     9361 ttccgcatat gctgctcaat cacttgccac aaagcccagc ctttgggatg ccaaaacacc
     9421 atacccggtg cttcgtcttg caagtggaac aaatccagtt gtttgccaag cttgcggtgg
     9481 tcgcgctttt cggcttcttc gatacgttga atataggctt tcaactcgtc ttttgtcgcc
     9541 caagccgtac cgtagatgcg ttgcagcatt tcattgttgc tgtcgccgcg ccagtatgcg
     9601 cccgccaact tggtcagttt gaagtttttc aggaaacggg tattcggaac gtgcgggccg
     9661 cggcacatat cgacatattc ctggtgatga tacatcccca tcgcttccac ttcgggcata
     9721 tcgtcaatca ggcgcagttt gtattcttcg ccgcgctctt gaaaaatttt aatcgcctcc
     9781 gcacgcggag tcatgatttt gaccacatca tagtcttggg caatcaattc tttcatacgc
     9841 gcttcaatgg cggcaacatc ttccggtgta aacggttttt ccgtggcgat gtcgtaataa
     9901 aagccctctt caatgacggg gccgataacc atttttgcat taggatagag ttgcttgacg
     9961 gcatgcccga caagatgcgc gcaggaatgg cggatgattt cgatgccttc ctgatctttc
    10021 ggagtaatga tttgaacagc agaatcttca acaatcgggt cgcacgcatc gaccaatttg
    10081 ccgtttacct tgcctgccac cgccgccttc gccaaaccgg caccgataga cgcagcaatt
    10141 tgagccacgg taacggggga ttcgtattgg cggactgagc cgtccggcaa ggtaatattc
    10201 aacatcaaac gctccgaaag attaaaaaat aacggcataa atgccgttat ggggatttgg
    10261 taggcacgat tggattcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac
    10321 tgagctacgt gccttcaaag aattttcgca ttttatcgag tcgttttaat ttttgcaaga
    10381 ggtatatgca tttctttatg caacattgtg gcaagatttg gggatgtcgt ctgaaaaacc
    10441 gtgggaattc atttataatg ccgtctttta aaacatattt cgacccctgt cgcccaactg
    10501 acattcaatc ttatgcttaa atttacttta cacaaaaaag acggatacgc ccgacgcggc
    10561 acgctggagt tgaaccacgg caaaattgaa acgccggtat ttatgcctgt cggtacttac
    10621 ggttcggtta aggcgatgaa cccgcaaaac ctgcacgaca tcaaggcgca aattattttg
    10681 ggcaacactt atcatttgtg gctgcgtccg ggcttggagg tcgtcgaaca gtttggaggg
    10741 cttcacggct ttatcggctg ggacaaaccg attttgaccg actcgggcgg ttttcaggtt
    10801 ttttctttgt cggatatgcg caagctgacg gaagaaggct gtacgttcaa aagcccgatc
    10861 aacggcgaca agctgttttt atcgcctgaa atttccatga aaatccaaac ggttttgaac
    10921 tcggacatcg cgatgcagct ggacgaatgc acgccgggcg aagcaacgcg cgaacaggcg
    10981 cgaaaatcgc tgcaaatgag cctgcgctgg gcggaacgga gcaaaaaagc ctttgaagat
    11041 ttgaaaaacc cgaacgcact gttcggcatc gtgcaaggcg cgatgtatga agatttgcgc
    11101 gaagagtcgc tgaaaggctt ggaagagctc gatttccccg gcttggccgt cggcggcttg
    11161 tccgttggcg aacctaagcc cgaaatgtac cgcatgttgc gcgccgtcgg ccctatcctg
    11221 ccggaacaca aacctcatta tttaatgggc gtcggtacgc ctgaagactt agtgtacggc
    11281 gtggcacacg gcgtggatat gttcgactgc gtcatgccta ctcgcaatgc gcgtaacggc
    11341 tggctgttta cccgttttgg cgatttgaaa atcaaaaacg ccaaacacaa gctcgacaag
    11401 cgtccgatag acgaaagctg cacctgctac gcctgccaaa acttcagccg cgcctacctg
    11461 caccatctgc accgcgccgg cgaaatcttg ggcgcacagt tgaacaccat ccacaatctg
    11521 catttctacc aagtcattat ggcggaaatg cgcgaagccg tcgaacaagg caaatttgcc
    11581 gactggcaag cacaattcca cgaaaaccgc gcacgcggca cagactgact ccctgcaatg
    11641 ccgtctgaag ccgcttcaga cggcattttg ccgcgttacc ctacccgcac tatttttcag
    11701 aaaccgatca tgctcccatt ccttccagaa ccctcccttt cctatacgca acaaaaccgt
    11761 accgccgttt tgctgttgaa cctcggcacg cccgacgcgc cgaccgcgca ggcggtacga
    11821 ccttatctga agtcctttct gaccgaccgg cgcgtcgtcg aactgcccaa atggctgtgg
    11881 tatcccattc tgcatggtct ggtactgacg ctccgcccca aaaaaagcgc gcacgcctat
    11941 gaaaaaatct ggtttaagga aggttcgccc ttagaagttt acacagcacg ccaagctgcc
    12001 gcgcttgcca aacgtatgcc cgacctcatc gtccgccacg ccatgaccta cggcaatcct
    12061 tcggtggcag acgtattgtc ggaactgaag gcgcaaggcg cgggcagact gctggtcatc
    12121 ccgatgtacc cgcaatatgc cgcttccagc agcggagcgg cagtagataa ggtgtgcgaa
    12181 caattactgc tgcaacggaa ccaaatgagc gtccgcaccg tttcgcgctt ttatgacgat
    12241 accggctaca ttgatgcgat gaaaaaccac atcctccgat attgggcgga acacggacgt
    12301 gggaaaaaac tgatgttgag ttttcacggc gtaccgcaga agcactacga cctcggcgac
    12361 ccctatcccg acgagtgccg ccacaccgcc aaactgcttg ccgaagcact ggaactgacc
    12421 gaagaccaat atgtcgtatc gttccaaagc caattcggca gggcaaaatg ggttacaccg
    12481 agcacgcagg atttgttcgg caaactgccc aaacaggggg taaccgagct ggacgtattc
    12541 tgcccgggct ttttggcaga ctgtttggaa accatggaag aaatcgccct gatggggcgg
    12601 gaacagtttt atgaagcagg cggcaaaagc taccgctaca tcccctgcct caacgacaat
    12661 cccgactgga tagacgcact cgtcgcactt gccgaagaaa accttggcgg ctggcgttga
    12721 aactgtttga cccatgtcta aaatgccgtc tgaacgccgt gttcagacgg cattttcccg
    12781 ctactttagc gtatgatggc atctatgctg gacaacacat caagtgcaac cttactaccg
    12841 attcggcaag aaaatctttc acccgctacg caaaatcaac aacaaggttt caaacattaa
    12901 ggaaacacca atgaacacaa cccgactgcc gaccgccctc gtcttgggct gcctctgcgc
    12961 cgccgcttct gccgccgaca acagcatcat gacaaaaggg caaaaagtgt acgaatccaa
    13021 ctgcgtcgcc tgccacggca aaaagggcga aggccgcgga accatgtttc cgccgctcta
    13081 ccgctccgac ttcatcatga aaaaaccgca ggtgctgctg cacagcatgg tcaaaggcat
    13141 caacggtaca atcaaagtca acggcaaaac ctacaacgga ttcatgcccg ccactgccat
    13201 cagcgatgcg gacattgccg ccgtcgccac ttatatcatg aacgcctttg acaacggcgg
    13261 cggaagcgtt accgaaaaag acgtaaaaca ggcaaaaaac aaaaaaaaac taaacagaca
    13321 aaatgccgtc tgaaaccggc aatccggctt cagacggcat tcaaatcaaa cctttctaac
    13381 gcggtacttt cagccccata gacccgatat tgccgtcccc gtcaacctcc ttcgcggtca
    13441 gcataaagcc gccgatgtca atacggccgc actcgaccgg ctcctgactg tcggaacgct
    13501 tatcgaaaag ctcggcaagg ctcaaaccct cttcgcccgc ttccagcctc aaaccgtaag
    13561 caagtgccaa atcacccgaa cgtgctgccg gcaaaacgac aaactcaccg aagaaatcaa
    13621 aatactcacg gacgctaata cccgtctcgg taaagtattt cgccatttta tagtgaa
//
LOCUS       DAWFKN010000054        13192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFKN010000054 DAWFKN010000000
VERSION     DAWFKN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.054"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(117..749)
                     /locus_tag="WGR46_001460"
     CDS             complement(117..749)
                     /locus_tag="WGR46_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856378.1"
                     /translation="MEKIEKFKSELLNAIFQYTQCISIFVYKKNIYYLIDCKENFILN
                     MKLDLDLDFKNGNITLEQYQDEMNSYYYRNGIWQLTKDNFESYLQSDSVIELKKDELK
                     KLMFQGFTSAEAVRLYSVVENKLSYNDPISDSGQQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(904..1398)
                     /locus_tag="WGR46_001461"
     CDS             complement(904..1398)
                     /locus_tag="WGR46_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ0856379.1"
                     /translation="MIFKQNQNYWAVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTKR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLDAWDAIDADDVILSLDNSPEEIGTGLRLAL
                     SRCR"
     gene            1722..3155
                     /locus_tag="WGR46_001462"
     CDS             1722..3155
                     /locus_tag="WGR46_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0856380.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            3264..4052
                     /locus_tag="WGR46_001463"
     CDS             3264..4052
                     /locus_tag="WGR46_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0856381.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            4420..5976
                     /locus_tag="WGR46_001464"
     CDS             4420..5976
                     /locus_tag="WGR46_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0856382.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            5993..6739
                     /gene="surE"
                     /locus_tag="WGR46_001465"
     CDS             5993..6739
                     /gene="surE"
                     /locus_tag="WGR46_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0856383.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            6995..8242
                     /locus_tag="WGR46_001466"
     CDS             6995..8242
                     /locus_tag="WGR46_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0856384.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPVAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(8449..8931)
                     /locus_tag="WGR46_001467"
     CDS             complement(8449..8931)
                     /locus_tag="WGR46_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0856385.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(9084..9245)
                     /locus_tag="WGR46_001468"
     CDS             complement(9084..9245)
                     /locus_tag="WGR46_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856386.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(9319..9780)
                     /gene="recX"
                     /locus_tag="WGR46_001469"
     CDS             complement(9319..9780)
                     /gene="recX"
                     /locus_tag="WGR46_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0856387.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(9848..10555)
                     /locus_tag="WGR46_001470"
     CDS             complement(9848..10555)
                     /locus_tag="WGR46_001470"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0856388.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            10962..12227
                     /locus_tag="WGR46_001471"
     CDS             10962..12227
                     /locus_tag="WGR46_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0856389.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(12285..13091)
                     /locus_tag="WGR46_001472"
     CDS             complement(12285..13091)
                     /locus_tag="WGR46_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0856390.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         3076 a         3184 c         3599 g         3333 t
ORIGIN      
        1 attttgcaaa ggtctcggcc tgagtgttgt tttacaggaa agtaaaggcc gtctgaaagg
       61 gtttaccagc tttcagacgg ccttttgaat tcatgcaagc tatggcttgc ttatatttat
      121 aattgtctaa atttccaata atcccgtcct tcaattttcc aataacaaaa ttcgtccggt
      181 attaaatacc caaaatccat cgcctttgaa aaccagccat cataaacata atcttcatga
      241 catctgtccc aatccatatg taagtacact tcagtgtcaa aattgacata gaataaaggc
      301 agcctcgagg aaatctgatt gatttttaag aaatcacttt gctgtccaga gtctgagata
      361 ggatcattgt acgacagctt gttctcgaca acggaatata accttaccgc ttcagcagaa
      421 gtaaatccct gaaacatcag tttcttcaat tcatcctttt ttaattcaat gactgaatct
      481 gattgaagat agctttcaaa attgtcctta gttaactgcc agatgccgtt tcggtaataa
      541 taactattca tttcatcctg atattgttct aacgtaatat taccgttttt aaaatctaaa
      601 tctaagtcta atttcatgtt taatatgaaa ttttccttac aatcaatcaa ataatatata
      661 tttttcttat agacaaaaat agatatgcat tgagtatatt gaaagatggc atttaataat
      721 tcagatttaa atttctcaat tttttccata tttgccaaat tccttaatag ctttatcttc
      781 agcaagtcgt agcccgcatt aaatctgcag gtaaggtgtg gtacgcacgc cagtgagctt
      841 gagtctaatg aaaaaggtcg tctgaaacat ttttccggct ttcagacgac cttttgtcaa
      901 atattatcgg cagcggctca atgctaacct taaacctgtt ccgatttctt caggactgtt
      961 atccaatgat aaaattacat cgtctgcatc aatggcatcc cacgcgtcca gcttgacatg
     1021 gcggctcggg ctgattttca ggcagccgtt gtgcagccaa atatccacgc tcatcatgtt
     1081 tttaaatagg gcgcgtttgg ttttatagcc caagttcccg catagcttgg caacccaatc
     1141 ctcatagcgt tgccgaattt tttcggtatc aaaaaaatct tggtcttctg gactgtcata
     1201 aacgaaagtc ctgctgtttg ccaatgcttg caagaccgtt gtgcctaaag tttcattgtc
     1261 ggtatccaat ggcaggatat gagggggata taggtggtct attgccgtta atcccaaacc
     1321 tgaacatgtt tgaacaatca gagcttcttc attcgtataa aaaactgccc aataattttg
     1381 attctgttta aaaatcatta ttttatctcc gtaattttga ctgtaatgtt ttgacttttg
     1441 ccatactcta ccacacgttg caactgcaat ctttgttcct tatatattat caaagggctg
     1501 cattactttt ctttctaaat taccattgta taaaaagtat ttttcttaaa cgacagggat
     1561 tgcgcccgcg ccatattcag acaccgcccg gatgttgcac tgcccgatcg gatgcttcag
     1621 acggcatcgg aagggtttgc agttttggaa tatgaagatg ataatgtccg cgagattgac
     1681 ggcatttgaa agcggttatt ccgcataccg gaggatacga aatgaacgaa tattaccaat
     1741 taatcaagca tcccgatatt tccctttccc cgatttcaga cggcatcggg gtcggcaatc
     1801 cggcaacggg cgaaattttg gcgtatgtcc gcaatacggg ttcggacaaa ctgaaaaacc
     1861 tgattcaaaa ggcggcggca gcgcaaaaat tatgggcggc gaaaactgct ttggaacgcg
     1921 ccgacatatt gtggcgttgg tattttttag ttaaagaaaa caaagaagca ctggcgcgcc
     1981 tgatgacgat ggagcagggc aaaagcctga ccgagtcgcg cggcgaaatt gattatgcgg
     2041 cttcgtttgt gcgctggttt gccgaagagg cgcggcggat tgacggcgat gtgttaacga
     2101 gtgtaaaagc gtcgcaaaaa ctggttgtgt tgaaacagcc tgtcggcgtt accgccgcga
     2161 ttacgccgtg gaacttccca tctgcaatga ttgcgcgcaa ggctgcgcct gctttggcgg
     2221 tgggttgcgc gatgatcatc aaacccgcat cgctcacgcc tttgagtgcg tatgccttgg
     2281 cttcgttggc ttacgaagcg ggcataccgc aggatttgtt gcccgtcgtc agcggccgcg
     2341 catcggaaat cggccgtgaa tttgccacga acccgattat ccgcaaaatc agcttcaccg
     2401 gttcgaccga agtcggcgcg aaaatttttg ccgacagcgc ggcggacatt aaaaaactca
     2461 gtttggaact gggcggcaac gcgccgttta tcgtgtttga cgatgccgat ttggacaaag
     2521 ccgtcgaagg tgcgctcgcc agcaagttcc gcaacagcgg tcagacctgc gtctgcacca
     2581 accgcgttta cgcccaatcc ggcatttacg acgaattttg tcgcaaattg agtgaaaaag
     2641 tagccgagct caaattgggc aacggcttgg aggatggcgt caaccaaggg ccgctgattg
     2701 aggaaaaagc ggtagaaaaa gtcgagcagc acattgctga cgcgctcgct aaaggcgcgg
     2761 tctgtctgac cggcggcaaa cgcagcgcat tgggcggaac gttttttgag ccgaccgtct
     2821 taagcggcgt aacggcgcaa atggcggtgg ctcgcgaaga aaccttcggg cctttgtgtc
     2881 cggtattccg ttttgaaacc gaagccgaag tcattgaggc tgcgaacaat acggaatacg
     2941 gtttggctgc ttaccttttt accgccgaca ccgcacgcca gtggcgcgtc ggcgaagcat
     3001 tggaatacgg tatggtcggc atcaatacgg gcttaatcag taatgaagcg gcaccgttcg
     3061 gcggcgtgaa acgcagcggt ttgggacgtg aaggcagcaa atacggtgcg gacgaatatc
     3121 tggaattgaa atatctgtgt atggatgtcg ggtgacggat gccgtctgaa cggcagggtt
     3181 tcagacggcg ccgcatttta agcagtctct atctgttgta caatgcgccc tgtttttacg
     3241 gttatttttg atttgaacaa gatatgatgg aaaacggaaa aacagtcccg agatggcgtt
     3301 ttgccttgaa aagtgcgggc tggcacctct taatcagcct gtcggttgca gggctggcgg
     3361 cattgctggt ttttaaggtt tggtatcctt atccttatgc cgagctgacg ggagggctgt
     3421 cgctttatca gctggtggtg gctgtcgata ttgtatgtgg tccgctgctg actttaattt
     3481 tggcaagccc gaagaaaaag acaaaggcac gcatggtcga tttttccatg gtcggcatca
     3541 tccagctggc ggctttggtg tacggtctgc acagcgtttc tttggcgcgc cctgtgatag
     3601 aggcgtttga aaaagacagg attaatgttg tgaccgccgc cgaagtggcg gttgaagatt
     3661 tgcacaaagc gccggaaggg ctgcaaagcc tgtcgtggtt cggcatccgc cgcattgcat
     3721 tgaaagaacc tgaggatgcg gatgagaaga acaagacgct ggatttgtcc ctgaaaggta
     3781 tcgagccgag tatgcgtccc gaccggtggc tgccgtattc cgacaaggaa gcagaaaaaa
     3841 tccgcaaaca tctgaaaccg ctgaaagtct tggcggatgc gagaaaaacg acggttgcgg
     3901 acattctgaa acaggcaggt ctcgccgaag gggaggagct gtattacctg ccgtttacca
     3961 gcagcaggca gaaagagtgg atagtcatta ccgataaaga gggcaacacc aaaggctacg
     4021 cgccgataga cggcttcatc atcacccctt aagcgttggg actccgtccg cactcgaaca
     4081 tccgttcttc gcggcggtag aatcagactg tatttgagag gggcgcaacc cctcaatcag
     4141 ggcaacacat aggggaaggc tttatgtgcc gtccgtgtgt tgaaacattt caaaataagc
     4201 tcatgccgtc cgaacatcct ttcagacggt atggcgtttt gccattgccc cgataagctg
     4261 ttaaactatt taaattattt cgaccgaagg tacacgcaca tgcaagaccc cgagcagagc
     4321 agcaagcccg cccgccgttt tttgtgcgta ccgtctgttc aggcagcttg agcgcttcag
     4381 gctgcctttt cgcgtttgaa cttgaggaat acgaaaatca tggatttcag ttggttggca
     4441 gaaccgcata cctggatagg ttttgccacg cttttggtgt tggaagtcgt attggggata
     4501 gacaatcttg tctttgtggc gattttggca aacaaggtcc agcccgcacg gcgcgaccgc
     4561 gcacggatta tcgggctggg gctggcagtc gtcatccgca tcattatgct tgcttttatg
     4621 gcgcacatca tcacgctgac cgagccgctg ttccaaatcg gcggcctcgc cgtttccggc
     4681 aaggacatga ttatgctcgc gggcggtatt ttcctgcttt acaaagccac caccgaactg
     4741 catgaacgcc tcgaagggca caaccgtttt accgttgccg acaaccaaaa aaaacacgcg
     4801 ccgttttggg gcgtggtcgc gcaaatcctg atactggatg ccgtgttttc catcgattcg
     4861 gtcattactg cggtggcgat ggtcgatcat atcgtcgtgg cgatgggtgc ggtcgtcgtc
     4921 gcgatggctg taatgatttc tgccagcaaa ctcttgaccg aatttgtcga cagacaccct
     4981 accgtcgtga tgctctgcct tggttttttg ttgatgatcg gtttcagcct gattgccgaa
     5041 gccttccatt tccacattcc caaaggctac ctctacgccg ccatcggctt ctcgatttta
     5101 atcgaattgt ttaaccagat ttcgcagcgc aacagccgca aaaacgacta catcggcagc
     5161 tcgtggcgca agcgcaccgc cgaaaacgtc ttgggtatga tgggtatacg cgaaagcgtg
     5221 cttgccgacg cgggcggcga atccggggac gacgcgcatt ttgaagaaaa cgaaaaatcg
     5281 atgatacgca gcgtgctgac gcttgccgaa cgcccgatta tgggggtgat gatcccacgc
     5341 cgcgacatcg aacggctgga catttcccaa agccgcgaag aacagtgtgc ccaactgcaa
     5401 aacacgcctt acagccgcct gctcgttgtc ggtaaggcgg gcgtggacga acctttgggc
     5461 tacatcaaca aaaaagacct gctgtcccaa ctgctggaaa caggcggtct cgacattcag
     5521 acggcattgc gccagccact cgtcctgccc gacagcacca ccgcgctggg cgcaatcgaa
     5581 ctcttccgcc aaagcagcgc ggattatgct ttggtggtgg acgagttcgg cgcggtattg
     5641 ggcatggtaa ccatgaaaga cctgctcgaa accatcgcag gcgagttccc cgaagaattt
     5701 gagcgcgaag aagaaccagc cgttcagggg aatcccgatg aaagcctgac ggtggaaggc
     5761 gcgttggaat atgtggaact cgcaccgcaa ctcaacctgc cgcagcagga ggaagatgcc
     5821 gatttccata cggttgccgg gctgattatg gaagaattgc aaaccatccc cgatgtcggc
     5881 gattttgccg atttccacgg ctggcggttt gaagtggtcg aaaaagaagg gcagcgcatc
     5941 gagcgggtca aaatcaccaa attgcccgaa gaataagcat tcaggataga aaatgaacgt
     6001 tttgatttcc aacgacgacg gctacctctc cgaaggtatt gccgttttgg cgcgcgttac
     6061 ggcggaattt gccaacgtca gggtggttgc gcccgaacgc gacaggagcg gggtcagcaa
     6121 ttcgctgacg ctggaacgcc ctttgcagtt gaaacaggcg caaaacgggt tctactatgt
     6181 caacggcacg ccgaccgact gtatccacat cgggcagtct gtattttcgg attttcaggc
     6241 cgattttgtc ttttcgggca tcaaccgggg cgcgaatatg ggggacgaca cgctttattc
     6301 ggggacggtt gcggcggcaa ccgaagccta ccttatgggc atacccgctg tggcgttttc
     6361 cttaaacgac gcttccggac gctattgggc aaccgcagaa aaggcactgt ggacattgtt
     6421 ggcgcatttt ttcaaaaacc ccccgcagtc ccctattttg tggaacatca atatccccgc
     6481 cgtcgagccg gaagatgtgc ggggcattaa aatcgcccgt ttgggcagga ggcatcacgg
     6541 tcagaacgtc atccccgcgc gcaatccgcg cggcgaacag atttattgga taggcccggt
     6601 aggcagggtt tccgacaaag aagagggaac ggatttcggt gaatgcggcg caggtttcat
     6661 taccgtaacg ccgctgcaaa tcgacctgac cgcctatccg gacatggcgg aaacagcggc
     6721 gttctggcat gcggactgac cgtttcatca aatatagtgg attaacaaaa accagtacgg
     6781 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
     6841 cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     6901 cactataccg tcgggctttg cgcttgagaa gaacgcgcat ttttattatg atataccgca
     6961 ttcaggctat atatcggaac atgaaggtta tcttatgttg aaacaaacga cacttttggc
     7021 ggcttgcgcc tcctttgccg cactgttggg cggttgcgcc acccaacagc ctgctcctgt
     7081 cattgcaggc aattcaggta tgcaggacgc gccgtcttcg gcagtttaca acaaccccta
     7141 tggagcaacg ccgtacagcc cggctcctgc cggcgatgcg ccttatgtgc cgccggtgca
     7201 aagcgcgccg gtttatacgc ctcctgctta tgttccgccg tctgcacctg ccgtttcggg
     7261 tacatacgtt ccttcttacg cacccgtcga catcaacgcg gcgacgcata ctattgtgcg
     7321 cggcgacacg gtgtacaaca tttccaaacg ctaccatatc tctcaagacg atttccgtgc
     7381 gtggaacggc atgaccgaca atacgttgag catcggtcag attgttaaag tcaaaccggc
     7441 aggatatgcc gcaccgaaag ccgcagccgt aaaaagcagg cccgccgtac cggttgccgc
     7501 gcaaccgccc gtacagtccg cacccgtcga catcaacgcg gcgacgcata ctattgtgcg
     7561 cggcgacacg gtgtacaaca tttccaaacg ctaccatatc tctcaagacg atttccgtgc
     7621 gtggaacggc atgaccgaca atatgttgag catcggtcag attgttaaag tcaaaccggc
     7681 aggatatgcc gcaccgaaaa cctcagccgt agaaagcagg cccgccgtac cggctgccgt
     7741 gcaaacccct gtgaaacccg ccgcgcaacc gcctgtgcag tccgcgccgc aacctgccgc
     7801 gcccgctgcg gaaaataaag cggttcccgc gcccgccccg caatctcctg ccgcttcgcc
     7861 ttccggaacg cgttcggtcg gcggcattgt ttggcagcgt ccgacgcaag gtaaagtggt
     7921 tgccgatttc ggcggcaaca acaagggtgt cgatattgca ggaaatgcgg gacaacccgt
     7981 tttggcggcg gctgacggca aagtggttta tgccggttcc ggtttgaggg gatacggaaa
     8041 cttggtcatc atccagcata attcttcctt cctgaccgca tacgggcaca accaaaaatt
     8101 actggtcggc gaaggtcagc aggtcaaacg cggtcagcag gttgccttga tgggcaatac
     8161 cgacgcttct agaacgcagc ttcatttcga ggtgcggcaa aacggcaaac cagttaatcc
     8221 gaacagctat atcgcgttct gactttctgc ctgaaatcaa gttggaaatg ccgtctgaaa
     8281 ggttttcaga cggcattttt gtttttggcg gctgtgtttt caaatcattc ggaagggacg
     8341 gggcatataa atttgtttga ctgtgtttgg gtatttgctt gatttcatcc gtttgcagta
     8401 gaaacggatg gcgcgatgtg cctgtctttc agacggcata gtccgccgtc agcagccgca
     8461 ggacatgtct tcagaggctt gcaggctgat gtctctgcgt tttttggctt tttcgtagcg
     8521 gcagcgttgg cggtcggtca ggatttccag cggagggaca gggacgggtt tgccgtcttt
     8581 gaccgcaacc atcgtgaagt agcagctgtt ggtatggcgg atttcgccgg tgcggatgtt
     8641 ttgtgcttcg acacggatgc cgatttccat cgaggtgcgt cccgtgtagt tgacagcggc
     8701 gtaaaaggta accaaatcgc cgatgtggat gggttcttta aacaggactt tgtcaaccga
     8761 cagggtaacg caataattgc cgctgtaacg gctggcgcag gaataggcga cttggtcgag
     8821 caggagcagg agttcgccgc cgtgtacgtt gccgctgaaa ttggcggtgt ccggcatcat
     8881 cagttcggac ataatgagtt cgtgggaagg cagttggcgt tgttgtgtca tagtgtacct
     8941 gttttaagaa agggtgtttt gaaatgggtg cggggaaatt ttagggaatg gggaagtgga
     9001 attcaagcta aaaaaagcta tgatgggata gtctgccgaa atggaccgcc gatgccgtct
     9061 gaagccttca gacggcattt tgtttaggaa agccaaaaac ggaagaccag gaaaaacacg
     9121 agtgccaata cgcccgccca aacgacgagc atgaatccag caactgcccg tcccatacgg
     9181 taaaccgggt ttttgcgttc gtcttcgatg ggggaggtgt ggtgtttttc gggttgtccg
     9241 gacattttgt tctcaatcgg aaaagttgga aacgggcgta aggttatgtt gccgtgcaaa
     9301 aagattcaag aaggaaggtc agcagccgtc ttcccaattt tcgtcccaag cgtgtttcaa
     9361 tgccgtctga acggtatcgg catcgaatcc gcgataggcg aggaaacgcg cctgtttttg
     9421 tttttctttg aggtcggcgg cggggtgttt gaatttttta cgcaacacgg ctatggcggc
     9481 cagtttttcg cttgagcggt cgggaagcag gttgcggctg gtttcttcat ctatgccctg
     9541 ttgcgccaaa gcctgtttca gcctcaatga accgtgtttg cggcttttgc tgcggatata
     9601 ggcttcggca tagcgcaaat ccgactgcca gttgcgttcg gcaaattcgt ttaacacgtt
     9661 ttccaactcc tcttcgcttt cggcgtgcgg tgcaagtttg cgtttcagac cgatgcggct
     9721 gagttcttgg cgcgagagga tgtccatcgc gcgggcgcgt agggattttt gcggtttcat
     9781 ggcggcggtt tcggcgtggg gcaggtttca gacggcatag cggcgcaaac cggagccgtc
     9841 cgaatgccta ctcttctttg tttttctgag gttgcaggtt ttcgtaaatt tcgggcagcg
     9901 agccgataat ccagtcgggg cgggtcgcat cgtcttgcga gagcagcgtc atatcgccgt
     9961 aaccgaaggt aacgccgacg ctcaggcagc cggcggcttt ggcggcgatg atgtcgttgc
    10021 gcgagtcgcc gaccataacc atgtttgcaa catcgatacc caaaacttcg gcggcgtgcc
    10081 gcagcggcag ggggctgggt tttttctcgg gcaggctgtc gccgccgagt atcaggctga
    10141 agtagtcggc aagtccgagc tgtttcaaca gttcgacggc aaggatttcg tttttattgg
    10201 taatgactgc cagcgggatg cccaaagatt tgagcaatgc cagcccggtt gcggtttcgg
    10261 gataggggcg ggtgaagacg ctcaaatggt cgcggtagta tttcatatag gatacgaaac
    10321 ctttttccca cagttcggaa tcggcttcgc ggtcgcggtc gttggtgagg acgcggtgaa
    10381 ccagtttgcc gatgccgtcg cccacatagc tttcgaccac tttggcgggc agcggtttca
    10441 taccgagttg ttccaacatc gcttctgcgg cggcggcaag gtcggggacg gaatcgcaca
    10501 gtgtgccgtc caaatcgaag gcgacggctt ggacgtgttc gatggctgca ttcataatct
    10561 gatttcctgt cgggttgaaa gggtttattt taacatcgtt tgcacagggc ttctaatgta
    10621 tagattgtta acaatcgcaa caggttttgc aggcggtgtt ttcggatttc ggggcggaat
    10681 cgggcaaggg tttgaaatcc cagcgatgtt ccaattaaat ttttaaaaaa taaaaggatg
    10741 cctatatctt tatttaattt gacatttatt tttcggacgc gcgcgctgct ttttcaggcg
    10801 gagtccgggc tgattccgtg cggcttgaaa aataaaatca tatatagatt aatcaaatcg
    10861 ggcttatgcc gactacacgc tattacattt tgtcgggaaa ttggttatag taatgagcga
    10921 aatgcacgcc cctgaaaatc tttttgtgaa aaggaagcaa aatgtctgaa gcccttgcaa
    10981 aagaaaccct caatccgttt gaaatcgcgc gcaaacaggt caaaaccgcc tgcgaccggc
    11041 taaaaaccga tccggccgtt tatgaaatcc tgaagagtcc gacccgcgtg ttggaagtca
    11101 attttcccgt caaactcgac gacgacacgg tcaaaacctt cacgggctac cgttcgcaac
    11161 acaacaacgc cgtcggcccc tataaaggcg gcgtgcgctt ccatcccgga gtgaatctgg
    11221 acgaagtcaa agccctgtcg atttggatga ccatcaaatg ctgcgtagcg ggcattcctt
    11281 acggcggcgg caaaggcggc gttaccttgg atccgcgcga ttattccgag gcggaactgg
    11341 aacgcatcgc ccgcgcttat tccgaagcca tttctccgct gatcggcgag aaaatcgata
    11401 ttcccgcacc tgatgtgaac accaacggca aaatcatgtc ttggatggtt gatgcctatg
    11461 aaaacgtggt gaaacattct gcaccgggcg tatttaccgg taaaccggtt gagttcggcg
    11521 gctctttggc gcgtaccgag gctaccggtt acggcgtgaa cctcgccgcc gtccaagcct
    11581 tggaaaaact gggcaaagac gtgaagggtg ccacttacgc catccaaggc ttcggtaatg
    11641 tcggttacca cacaggttat tacgcgcatc aatccggcgc gaaagttgtt gcggtttcta
    11701 ctgttgacgt tgccatttac aacgaaaacg gcttggatat ggaagctttg tttaaagagt
    11761 tccaagaaaa aggcttcatc accaacgaag ccggttacgg caaggaaatt accaacgccg
    11821 aacttttggc tttggatgtg gacgtactcg ccccttgcgc gctggaaaat caactgactt
    11881 ctgaaaacgc cggtaaagtc cgcgcgaaaa tcgtggtcga aggcgcgaac ggcccgacta
    11941 cgcccgaagc cgacgttatc ctgcgtcaaa acggcgtatt ggtcgtgccc gatattttag
    12001 cgaactgcgg cggcgtggtg gtatcttatt ttgaatgggt acaaaatctg caaggctatt
    12061 attgggagtt tgacgaagtt caggaaaaag aaaccgtcgt cctgcgccgt gcgttccgcg
    12121 atatatggaa cttggcgcaa gagtatgatg tcgatttgcg aaccgcgtct tacatgatga
    12181 gtatccgccg tgttgaaaaa gcgatgaaac tgcgcggttg gtattgatgt ttgaacaaaa
    12241 aatgccgtct gaagccttca gacggcattt ttgtgcaggc gggattaatg gtgcgaatgg
    12301 ccgatttgga aggttaaagt cgagtagttc gcctgttttt ggcacacgct ttgatcgggg
    12361 aagtcggctt tgtgttcgac attggctttc cagaagcctt ggcgcaaggg gatgatgtcc
    12421 acttcgcctt tgtcgtctgt gctgtcggag aaagcctgtg cttcggtttt gtgcgttttg
    12481 ctgcggtcgc tggtgtcgaa gccgtcaaag gtggcggtaa cggtggcatt gggcagcggt
    12541 tcgccacgga acagaacgcg gactttgaag cgttcgccta cgtgaatgtt ggcgggattg
    12601 tccagcggga cgatttccaa gttttgtccg accggtttgg tgatgatggc ggtgtccgcg
    12661 ctttcgtgtc cgacgttgac gatgtttttg ccgaacattc gggtttgttc gcaatagctt
    12721 gcgtcaggca tttctttgat gcccgcctgt ttccagcctg ctttgttttt tgaccagaaa
    12781 gtaggctgat attcggcgat gacgaggtaa ctgccgtcct taacgggacg gttgcttcgg
    12841 tactggtagt tgtatgtgcc gcgttgaatc atgttttcct tgcctttttc ggtaaccagc
    12901 tgcatcggtt tgctgaagat gtgcaggcgg tctttggcga tgggttcgag ttcgggaaat
    12961 tcgccgtagc ccaagtcggc tttaaggtat tcgccgccgt gcgtgtgggc ggtttcgacc
    13021 cagacgcggt gggcgtgggc ggatgtggca aatagggcgg aaacggcgag cagtgtcaat
    13081 gttttcttca tggtgcagcc tttgtctcgt gtgggttaaa gattttgtta tgttataaca
    13141 aaataggtat cgtgtgaaga tggatttgct gtctgaaaac tgtctttttg ta
//
LOCUS       DAWFKN010000055        13105 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFKN010000055 DAWFKN010000000
VERSION     DAWFKN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.055"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..440)
                     /locus_tag="WGR46_001473"
     CDS             complement(<1..440)
                     /locus_tag="WGR46_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856391.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFF"
     gene            complement(608..808)
                     /locus_tag="WGR46_001474"
     CDS             complement(608..808)
                     /locus_tag="WGR46_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856392.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(923..1342)
                     /locus_tag="WGR46_001475"
     CDS             complement(923..1342)
                     /locus_tag="WGR46_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856393.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(1400..1765)
                     /locus_tag="WGR46_001476"
     CDS             complement(1400..1765)
                     /locus_tag="WGR46_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856394.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(1747..2292)
                     /locus_tag="WGR46_001477"
     CDS             complement(1747..2292)
                     /locus_tag="WGR46_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856395.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(2455..2726)
                     /locus_tag="WGR46_001478"
                     /pseudo
     CDS             complement(2455..2726)
                     /locus_tag="WGR46_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3288..3760)
                     /locus_tag="WGR46_001479"
                     /pseudo
     CDS             complement(3288..3760)
                     /locus_tag="WGR46_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3849..4073)
                     /locus_tag="WGR46_001480"
     CDS             complement(3849..4073)
                     /locus_tag="WGR46_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856396.1"
                     /translation="MMKNKYILTKAAIGITAISIFYLLNQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
     gene            complement(4250..4858)
                     /locus_tag="WGR46_001481"
     CDS             complement(4250..4858)
                     /locus_tag="WGR46_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0856397.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIPVKYSGLTKIRTRRRSRRQYK"
     gene            complement(4890..5273)
                     /locus_tag="WGR46_001482"
     CDS             complement(4890..5273)
                     /locus_tag="WGR46_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0856398.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            5642..6346
                     /locus_tag="WGR46_001483"
     CDS             5642..6346
                     /locus_tag="WGR46_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0856399.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(6410..6976)
                     /locus_tag="WGR46_001484"
     CDS             complement(6410..6976)
                     /locus_tag="WGR46_001484"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0856400.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7042..7458)
                     /locus_tag="WGR46_001485"
     CDS             complement(7042..7458)
                     /locus_tag="WGR46_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856401.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(7510..8409)
                     /locus_tag="WGR46_001486"
     CDS             complement(7510..8409)
                     /locus_tag="WGR46_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0856402.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(8440..9897)
                     /gene="der"
                     /locus_tag="WGR46_001487"
     CDS             complement(8440..9897)
                     /gene="der"
                     /locus_tag="WGR46_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0856403.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQTAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(10051..10680)
                     /locus_tag="WGR46_001488"
     CDS             complement(10051..10680)
                     /locus_tag="WGR46_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0856404.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(10681..11976)
                     /gene="hisS"
                     /locus_tag="WGR46_001489"
     CDS             complement(10681..11976)
                     /gene="hisS"
                     /locus_tag="WGR46_001489"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0856405.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNFLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(12070..12612)
                     /locus_tag="WGR46_001490"
     CDS             complement(12070..12612)
                     /locus_tag="WGR46_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0856406.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
BASE COUNT         3324 a         3102 c         3005 g         3674 t
ORIGIN      
        1 aaaaaaaaat aatttttgca ttgattttta atagatttaa aattcgaata tagtgtttct
       61 ccagatttaa aaagtttatc tttatctata tatttgatgc tttccctatc caaaataaat
      121 atttcaaaca ttaaaaaatc attacatgac caagccaaat cataaatttg attcaaatgg
      181 gtatcataaa gataaaaata ataaggtttg ggaacaggta aaatatttaa tggaaaagga
      241 ggactaattt tcttaatatc tgatgactga tatccatatc tttctatatt cttaaaaatt
      301 tcatctttct taagataaca agacattctg acaatcacaa cctgttcatc ggatatatta
      361 tctatttgca tggcgcgtat aacacgccat gcctgattaa aattagtctc ccttacctta
      421 gaatctatta tctttttcaa agatgaaaat gccccttatc ttgaactctc cagttagaac
      481 ctggaagttt cgtacctctt aaatgtgtat taatatttct tatagtttca ttaaaatgcc
      541 acgcgctgcc gatttcaacg gtaattttcg taccgctttc ttcaaattcc aagtattccc
      601 ccattagcta acgcaaagaa gcagacgcca tttcggcttc gttatgataa acccgccttc
      661 cgttgatata gacttccgcc cctgtccgct ccaaattcca aaaattccgt actgaaattt
      721 ccatatcccg atattgtgca gaccatgttt tttcgaaggt tttcataaaa tttcctatac
      781 ctgtccaatc ggcacatatc aattgcatta ttatagtgga ttaacaaaaa tcaggacaag
      841 gcaacgaagc cgcagacagt acaaatagta cagaaccgat tcacttggtg tttcagcacc
      901 ttagagaatc gttctctttg agctaaggtg aggcaacgcc gtactggttt aaatttaatc
      961 cgctatacat ctcaatacga taaatattcc ttaagtcaaa atgcaagcct gaccgtacct
     1021 taactgtcaa aattttatta ttttttattg attttaaaac aatttctgca aaattctctt
     1081 cgctttctct cttttttaga agcacataag aaaaaataaa acttccccga ttaaattcat
     1141 aaatatgttt caaccattcg cctcctcttt ctgtaagaca agattcagtt tcattcttcc
     1201 ttattgtata aatatttcct tcacaaaatc tgaaataaat ccataaatcc atcttatcca
     1261 taattaaaga aaaagtttca cctcgagatt ttgtcaacaa ttcgcaaggt tgcgatgttg
     1321 caatcaaata gccgaaagac attttttacc tcatacatgg tcgaaatcag tttctgttag
     1381 ttcagaatcc attttttcgt caacaactga atccgcattt ttgaattaac gttttcatca
     1441 gctgccgttt atctaaaccg gcaggttcag tttcaaaata agccttatat gaagactgta
     1501 agcatttcag aaaaagatca tcagaagaca tatctgccga atcaaataca actgttttga
     1561 ttttggtact tacccaaaac cctttttgct ctttttctac tatacggaaa ttcagaatat
     1621 ttccaaccga atcaaaagca cggtaaacat catccatcaa atcctgcggc tctattttct
     1681 tttccaattc cgacaatcct tgaaatatat ccaaagacac atcttcaaat agaaaaaaag
     1741 gaggagttag aagcggtttt tccatgatct gtccgtagat tttgattccc aagggcgatg
     1801 acgaccaatt ccctgtccag gcaaagtctt gcccgtatta tccgtaactc gacgatgata
     1861 atggggaaat tttccaatag gatgacctgt tctattaccg aaaggggcta tccgcatatt
     1921 attgccgatt ttaatctcac gtccatattt agcaaaggaa acaacctttc ctgcggcgcc
     1981 tacaccacca ggaattgcgc ctaatccgcc agcaatagca acatctctaa cagaagctgg
     2041 tctgcctgtc gttgcataac taaaaccatg ctgtgtccac ataccaatgg cagcaccacc
     2101 caagatagcc aatggaagaa acgccctctc agtctccttc atctcctttt gagaaagttc
     2161 cgccaactgc atcggcgcat ctgcccgcgt gtggaacact tggtcttcaa atgcctgatt
     2221 gtccaagccg tttgccattg cgggggcaat catagacagc atcattacgg ctgcggtgat
     2281 ttgttttttc ataataactc ctttggatta caaggttgga aaatcaaagc cctgcttaga
     2341 acgtatgttg cacacccaat ttcaataggt aaatcagatt gcaaatccag caatttgaat
     2401 attgtcattg ttccgtgcaa aagggatttt tattgatgag ttgtgtactg ggtttcagct
     2461 tggcttttta gataatcttt tcacaagaac atacatcaat actgtccaaa aaaaagattt
     2521 gataaatata ttgcaacata ttttattcca cctccatccg aaacagaaac caatatattt
     2581 ttaattaata catagctgat tattacattt attattccta taatatataa ggatcttgcc
     2641 aaaatttttt ttgattttat tttaggaata tctcttatcc atgctaaaat acagcccaat
     2701 gtcgaaaaga aaataacagc aaacactacc ttacctcctc tcacttccaa ccaaaccgat
     2761 agcaggtttg gttggaagtt ggtttattta ttatttaacg gcgaatgtca gtgttcttac
     2821 ccgtagaacc tgcataacca tttatgccac cagcaacagt ccccactgct acacctcgca
     2881 taccattaat cagaattctt cctatttttt cgaatccact atttcttcca aagatgcagc
     2941 aaacgcaggt tgagcctaga cccaatctac gtttgctgcc gcatctgatc cctattgttt
     3001 cttatcctta catcttcctg ccttgtcaat caaataaaga cagaagacta tacaaaaact
     3061 gaccgacata ctgaaaatac ctatcccctt ccatgcaatc tccgcaccat tgacccaata
     3121 gattagactg agaaacaaca aagccacagt ataaatcaac gtaaaaaata ttgcgtaaag
     3181 cacaataaag ggaactttta tctcacggtt gttttttata atatattcag caacttgatt
     3241 tccgaatata cctgataaaa aatatagtat tagatcatcc atttcgttta tcttctatgt
     3301 tttcccattg cggcgctaga agactgattt aatgctgcac catttgcacg aataactgca
     3361 ttaggaatag catttccccc tttccaagca gaacgggcaa aaacactagt agtaatccct
     3421 gcaccgcgca acatcgcact gctatatccg ccgcctatca taccaccagc ggttgcagcc
     3481 aaggtacttc ttgttgaagc aaattgccct gttttaattt tagaaatgcc gtgattagcc
     3541 caagcactaa ttgcccctcc catcaaagca cctgccacaa taggaagaaa cgccccctca
     3601 gtctccttca tctccttttg agaaagctcc gccaactgca tcggcgcatc tgcccgcgtg
     3661 tggaacactt ggtcttcaaa tgcctgattg tccaagccgt ttgccattgc ggggcgatta
     3721 tagacagcat catcgtggct tcagtgattt gttttttcat aacaaatttt tagggaggag
     3781 ataatgttcc atcaaaatcc tcctgaaagg ctgcctgaaa tttccaagca gcctttttga
     3841 ttgcttaatt aatcggatag tgcgacaaat ataaagtggg cggcaattat tgccgtaacg
     3901 gcagccataa ctgttttatt ttcttcaaac caaagcaagt tgagaaacat aaacatcaag
     3961 aaatatgatg gttcttcagt tttgccgctg ctttcttgat ttagaagata aaatatagat
     4021 atggcggtta tgccgattgc tgcttttgtt aaaatatatt tgtttttcat catcttgtat
     4081 ggtttttcat gcaagtttca acaataaaat tcggaataag atagagagat gttgatatat
     4141 cagataaata tatagtggat taaatttaaa ccagtacagc gttgcctcgc cttagctcaa
     4201 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact
     4261 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatttc accggtattt
     4321 tcttaccgta ttctgcgatt gcctgtcgga aaatgccgat caacctgcct ataacggcat
     4381 tttcgccaaa ttcgttcaga cagttttccc taagtcgggc aggttcaaaa tcagagtggt
     4441 gttcatacat tttgatgagt gcgtcggcaa gggcatcgtc gtcgtcaaca ggaaccaaat
     4501 atccgttgcc gtctgaaaca atagattccg caccgccgca gcgtgtcgca atgacgggca
     4561 atccttggga cagtgcttcg atatagacca cgccgaaggt ttcggtgcgg ctggcaagga
     4621 cgaatgcgtc gctgttcctc atcaaatcca agactgcttc gggctgcaat gcgcccaaaa
     4681 atgtaacggc atgggtaatg cccaagtctg ccgcctgctg tttcagccgc tgttcttcct
     4741 gtccgctgcc gccgatgttc aggcgcagtt gcgggcattg tgccaacgcc cgggcaaagg
     4801 cagtgagtag gacatcgtgt cctttgagac ggcgaaggtg cgagacggtg cagaacacga
     4861 aatgcggttt gttgtttttt tctctttgtt caaacggtcg gttgaatatt ccgcccagta
     4921 tgttggggga ggtactgcca ttcgcagccg tatttgtgtt gcaggacgtg tgcgaaatgg
     4981 cggctgacag cgagcagtgc ggaggcgtgc gccgctgctt tttccatcgg ctgccattgg
     5041 tggggacgga tcagaccgcg cgtgatggtg ctgctgtgtt ccgtgacgac ataggggatg
     5101 ccgtattttt gggaaatttt ttgggcgagt atgccggcat agttcatgcg gtgggcgtga
     5161 atcaggtcgg gcagcccgtt ttcgcggatg tagtgtttga aagctttcaa acccgcacac
     5221 acccagcgga tgcagtcgat gtcgatgaac ggaaagcggg ggaagaaata tatgccgtgc
     5281 catgcataga tgtccaaacc gctttgccga tattttgcaa aaccgtaagg ccggtcagga
     5341 tgcttgctgt ttctttccgc aagtagcgga acatcggtgc aagcacggcg gttttgatgc
     5401 ctttcctctg caatgccagt gcctgatttt gaaaaaaatc ccgtccacat cctgttcgga
     5461 ttgcggatac catgagggga tgacgaggac gtgcaagggt tcgggcatag tgggattccg
     5521 tatcggaaag gcggttatta taagacagac gcagaccgaa tatttaaatt gttgccttac
     5581 gctaatgcaa tttagcgcgc cgatatgcta gattggcggt tttataagta aggatacgga
     5641 tatgttgcgt tctattttgg cggcttccct gctggcggta tcttttccga cggcggctga
     5701 agcattgaat tacaatattg tcgaattttc tgaatcggcg ggtgtcgagg cggttcagga
     5761 tacaatgtcc gcacgtttcc aagtgacggc ggaaggacgg gacaaaaatg ccgtcaatgc
     5821 cgagtttgtt aaaaaattca acaatttcac cagaaaatca aaaaatggta gctttaaaac
     5881 cgaattggta tcgcgcagtg cgatgccgcg ctatcaatat accaacggca gacgcattca
     5941 aacaggttgg gaggagcgtg cggaatttaa ggtcgagggt agggattttg atgcgttgaa
     6001 ccgttttatt gccgatgttc aggcagatgc cgcgttggaa tatacggatt tccatgtgtc
     6061 gcgcgaacgc cgcaacgagg tcatcgatca ggtcagcaag gatgccgttt tgcgtttcaa
     6121 ggcgcgtgcc gaaaagttgg cgggcgtttt gggtgcgtcc ggttataaaa tcgtcaaatt
     6181 gaatttggga cacatcggca gccatatcgc gggaggggga gctgctcagg caaaaatgct
     6241 tcgtgccatg ccgatggcgg caagcgtcaa tatggagggt gcggattccg ccgcgcctgg
     6301 tgtggaggaa atcagcatca gcgtcaatgg gacggttcag ttctgatttg aggtgaacgg
     6361 caaatgccgt ctgaaacccg acgataaggg ttcagacggc atttatattt caggctttgg
     6421 gcagggtaac gccggtttgc cccatatatt tgccgttgcg gtctttgtat gaggtttcgc
     6481 agatttcgtc gctctcgaag aagaggactt gcgccacgcc ttcgcctgcg tagattttgg
     6541 cgggcagggg ggtggtgttg gaaaattcga gggtaacgta gccttcccat tccggttcga
     6601 acggggtaac gttgacgata atgccgcagc gggcgtaggt ggatttgccc aagcagacgg
     6661 tcaggacgtt gcgcgggatg cggaaatatt cgaccgtgcg cgccagtgcg aaggaattgg
     6721 gcgggatgat gcagcagtcg tcttcaacgg taacgaagtt tttcgggtcg aagtttttgg
     6781 gatcgacgat ggtgctgttg atgttggtga agattttaaa ttcgtttgcg cagcggatgt
     6841 cgtagccgta gctggacgta ccgtaggaga tgatgcgttt gccgtcggct tctttgattt
     6901 ggttcggctc gaaagggtcg atcatgccga attcttcgct cattcggcgt atccatttgt
     6961 cggacttgat gctcataatg ttttccttgt ttcttgcagt gttcggacaa agcattgggg
     7021 gatgccgtct gaaaacgggg cttatttgtt tttgggcagc ttcacttctt taatcatgcc
     7081 gttttcgcat ttcataatga agagggtttc gccgttttgc gagacggtaa atctgccgtg
     7141 tgccaagcct tttttgaatg tgcccgagag taccatgttg cggaatttgg tactgtcgga
     7201 attgaacggt tcgataaata tttcgcggtt ggcggcaacg gtataaacgc cttgtccgtc
     7261 gaatttgcca tttttaaacg aaccggtata gttgcgcccg tcttggcagc gccatgtgcc
     7321 tttgccggcg ggtttaccgt ctttgccgac atcgccttcg taggtgcagc cgggttcttg
     7381 ataggaagtc agaacggcgg acgcggcggg gagggcgaac atcatggcgg gcagtaggaa
     7441 tgcgagatgt ttaagcataa gggttattcc attggatttt ggctgacggt ataaaagccg
     7501 tctgaaaaat caatcttgcc agccgcccaa ataggaaacc agctcttcca acatgatgct
     7561 gacggattcc gccatcagga tttgcgaggc gaaggcgaga ccggcggcat cgtcgccgtt
     7621 gctttcggct tcttcctgca atacgtcgag gtattggatg cgcttgagtg tgaagtcttg
     7681 cgtgaggata aaggcaattt gttcgcgcca caccaagccc agttgggtaa cggttttacc
     7741 gtttttgacg tgttgaacca cttcgtcggc ggttaaatct tgtttggata ctttgacgac
     7801 gggaacaata tcgcccgtgc ctttgagttc gcaatcgctg tccaattcaa aaccgccttc
     7861 gcaatgccct tgcaacagcc agccggtcat cagggaagag ggcgattgtt tggtattcgg
     7921 cagcgaggct tccaaaccgc ccaaagcttc gcgcagcttg gtcaggatgt tttccgcttt
     7981 ggcggaagcc gcgttattga cgagcaggta gccgtggcgg gtgttaaaca ccgcttctgt
     8041 acggctgctg cgggtaaacg ctcggggcag caggtcgtct gtaatttgct ctttaagctc
     8101 ttgtttttct ttgcgaccga cattgcgggc ttcattgttt tggatttccg ctaccttctc
     8161 ttccaaaata tcgcggatga cgccggcagg caggactttt tcctcttttt tcagggcgac
     8221 gcgcaaggta aagtcggcag ggaaaacgag ttccggggag aatgaaaccg gtgcggtaaa
     8281 gccttcgctg aaccagtcta agccttggca atgggtaaat tcagcttcag caagtttgtc
     8341 ggcaagtacg tccgcctcag gcagcttttc tttgttgagc ggataaaaac taatctgctt
     8401 gaaccacata atgtttcctg ttgtttgaaa tgtcgggaat tatttgctga attgtttttt
     8461 cacactgact ttggttttct tcttgaagcg gtttttctct tctttgcggc cttcgcgttt
     8521 ctcaatacgg ttgctcaggc tgacgcggcg cagcggtttt ttcttcggtt tgtcgtcggc
     8581 gttttcatac gggttttccg aaacattgta ttgaatccgc aacggcgtgc cttgcaggtt
     8641 gaaggctttg cggaacgttt gggtcagata gcgcgtatag ctgtcggaaa tcgcgtgcag
     8701 cgaattgccg tgtaccacaa ttacgggagg gttcatgccg ccttggtggg cataacgcat
     8761 tttcggacgc accaagccgg cacgcggcgg ttgctgacgc tcgattgcgc tttgcaggac
     8821 gcgcgtgatt ttcggcgtcg gcatcttaat catcgccgcg ttgtaggcag tctgaatgct
     8881 gtcaaacaaa ccgtctatac cgcgctcttt caatgcggaa ataaagtgga acttggcaaa
     8941 atcgaggaaa tacagtttgc ggttgatatc gcgtttcact tgctcgcgac gttcttcgct
     9001 gatgccgtcc catttattga ccgccaccac caaagcacgg cctgcctcca aagcgaaacc
     9061 tgcaatcgtc gcatcttggt cggcgatgtc ctgctgcgcg tccaatacca aaacagcgac
     9121 gtttgccgct tcaaccgcct gcatcgcttt gataacggag aatttttcca ctgcttcatc
     9181 cactttgccg cgacggcgca cacctgcggt atcgatgatg gtaaacggtt tgccttcgcg
     9241 ctcgaaatcg atatggatac tgtcgcgcgt cgtacctgcc atatcgaagg cgatgacgcg
     9301 ctcttcgccg agaatggcgt ttaccaaggt tgatttaccg acatttggac gaccgataac
     9361 ggcaaaaaca ggatgtttcg cctcttcctc ttctttttca ggctcaggga aggtttccaa
     9421 aatatcttca atcagataat acacgccgtc accgtgcgca cctgaaataa cataagggtc
     9481 gcccaaagca agttcgtaga actcggcggc aagcacggcc ctgttgccgc cttctccttt
     9541 attcacggcc aaataaacgg ggcgcgggct ttggcgcaaa cggtcggcaa tgattttatc
     9601 ttgcggcgtc agaccggtac ggccgtccac caaaaacaca accgcatcgg cttcatcgac
     9661 agcctgtaag gtttgttttg ccatttcgtg caaaatgccg ctgtccacaa ccggctcgaa
     9721 accgccggta tcgatgacca aataaggttt gctgccgact ttgccgtgtc cgtaatggcg
     9781 gtcgcgggtc aggccgggca ggtcgtgtac gagtgcgtct ttggtgcgcg tcaaacggtt
     9841 gaataaagta gatttgccga cgttggggcg gccgacaagc gcgatggttg gtttcatgat
     9901 tgagtctttc tgtgtcaagt gccgttcggg agaactgaac acgagcaggt gtccgttgga
     9961 cacggtaaat aggttcttca cggcaagagg ccataaagag ggagggtaaa atgccgtctg
    10021 ataaataatg gacggcattt cagacggcat ttatttcagc gaatcaagtt tcatttgaac
    10081 caattcgcga ccgacagaat cttgaggcat tttttctaaa gcctgtccgt agttttttaa
    10141 ggcttcctgg ctttttccct gtgcggcata gacatcgcct ttggtttcca tcagcagggg
    10201 ggcgaagtcc gcttcaaccg gcgtgtcgag tgcggcaagc gcggcatcgt attttttttg
    10261 ttgcaacaac acaacgccca gacgctgcgc cgccaacgcc tggatcaggc tgtctttttg
    10321 gttggacaat acccatttca aatggccttc ggcaacatcg taacgctgcg cgtcaaattc
    10381 ggttgctgcc gccatcagcg tggcttgggc ggcggaaatg gaatgggggt agctttgttg
    10441 gagcttggcc aattcggcat tgatttcgct ttgcggggct ttgttttgcg ccttttccac
    10501 gatgtttgcc agcaccgccg ccgcttcctg attttgggaa gccgcacggt tttggtaaac
    10561 cgtgtatccc aagtagccga gtgccgccaa aatcagcacg gcaaacagcc atttgcccgt
    10621 ggttttccaa aaatatttaa agttgtccaa ctcttgttgt tcttccaaat gggctgccat
    10681 ttatgcgttc ttccattgtt gtaaagtgtt tgtcaaatca gcggcggcga cggtttgctg
    10741 accgtgtgcg ccgttcatat ctttgagcgt aaccgtaccg ttcgccagtt cgtcttgcgc
    10801 gacaatcagg gcaaagcgtg cgccgctgtt gtcggctttt ttcatttgcg ctttcaggct
    10861 ctgatagccg gaatgctgca tcacattgaa accttgcgcg cgcaaggctt gtgcgtattt
    10921 catcacctgc aagtccgccc cttcgccttg gtgcattgca tagacatcag gcgcggcgtt
    10981 tacttccagc gagccgtatt cgctcaccaa aagcagcagc cgctcgatgc ccattgcaaa
    11041 gccgatagac ggcgcaggct taccaccaag ttcctcaatc aaaccgtcgt aacggccgcc
    11101 gccgcacaca gttgcctgcg cgccgagttt gtcggtcgtc cactcaaaaa ccgtttgatt
    11161 gtaataatcc aaaccgcgaa ccaagcgcgg attttcaata tattggatac ccaaaccgtc
    11221 caacatcgct ttgaagcgtg catagtggtt ttgcgaagcc tcgcccaaat aatccaccag
    11281 acgcggtgcc gcattgcaga tttcctgcaa atcagggttt ttcgtatcca aaacgcgcaa
    11341 aggattggtt ttcagacggc gtttgctgtc ttcatccaat ttatcttcat aacgggtcag
    11401 atattcaacc aatgccgcac ggtgtgccgc gcgttcctca cggttgccca agctgttgat
    11461 ttccaaagtc aggtattcgc ggatacccaa tttttcccac aagtcggcag acatcgcgat
    11521 aatttccgca tcgatatccg gcccttcaaa acccaaagcc tcgataccga cctgatggaa
    11581 ctgacgataa cggccttttt gcggacgctc gcggcggaac atcggcccca tataccacag
    11641 cttttgcggg ctgttgtaca gaaagttgtg ttcgaccacc gcacgcaggc aggaagccgt
    11701 accttcagga cgcaagctca aactcaaaga atcgtttgaa tcggagaagg tgtacatttc
    11761 cttgccgacc acatcggttt cctcgccgat ggagcggaca aacaaaccgg tttgctcgac
    11821 aatcggcgta cggatttgct gataaccgta agtgcgtgtc cagcggccga ccgtatcttc
    11881 aaacgcctgc caaaacgcag ccgtcagttt gaaatctttt tgctcgacag gcagaaggtc
    11941 gttcatgcct ttgacggatt ggattttttg tgccatttca agtaagaatg cttaaatcaa
    12001 attgcgggcg attatagcgg atttttaagg gtttgtgagg ttgggggtgg tttgcggacg
    12061 gcatttgact tactctgcac gtgcttgcct gatttgtccg actgcaaact ccgtctgccg
    12121 cttgggatga tgtgtgaaaa acaatttatt tgaaattgtc tcggcttttt tcggcacgac
    12181 cgccaaaatc ttacctgcca aatttccctc acgggtttgc caagcatcca aaaactgcgc
    12241 cctgctcatt gaaacatgac ccagcgacgg gtcggcaagc aaaaccgtat tgccgtctat
    12301 cccgcgcaat accgagaaat gatcatcctt gcggtatttc agatacacga tgacggggat
    12361 tttcaactgt gcgagctgct cgaaagacag ggcatagcct ttcgcttcaa aacccaaatc
    12421 tggcataatg cgccgcatat cctcaaacga cgcgcgcatc tgctccttat ccagcttttt
    12481 caacacttct tcttccgtca gcgtttgccc gtaaaaattg ttcaaaagcg tcgccaccga
    12541 agccgccccg caggaaaaat ccaaatcctg ctttacaata ttgaaatccc gcctttcttt
    12601 ccaactctgc actttgattt ttccgtaaac aacaggatta tcgttaaaca tcggtgcagc
    12661 attcaaacga taagataaag aaacgacaac acacgccaac agaaaaacat atttgaactt
    12721 catcatattg tccacataaa gggcagcctg aaaatctttc aggctgccct tgtcaaatta
    12781 ttcctagctt tcggcttttt tggcaaacca aacaatccga ttacccgcat aatactttcc
    12841 atttattgaa atccgacaag ccgcgcccaa aaaatgccat gcactgtcga tttccgcagt
    12901 aatctttgta tcgttttctt caaattccaa atattcaccc aataataaac ttgaaacaga
    12961 acgcgtgggc agcaagtgcc caccctacgc ctattcaaat aatttgatta aataaagaat
    13021 aaagaaaatg catcaggaac aaaattataa tctgccacct gactcacacc gcttttaaag
    13081 taaggggcat caaaatcaaa accgc
//
LOCUS       DAWFKN010000056        12920 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFKN010000056 DAWFKN010000000
VERSION     DAWFKN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.056"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            733..858
                     /locus_tag="WGR46_001491"
                     /pseudo
     CDS             733..858
                     /locus_tag="WGR46_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1009..2052)
                     /locus_tag="WGR46_001492"
     CDS             complement(1009..2052)
                     /locus_tag="WGR46_001492"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0856407.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(2049..2342)
                     /locus_tag="WGR46_001493"
     CDS             complement(2049..2342)
                     /locus_tag="WGR46_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856408.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            2596..3750
                     /locus_tag="WGR46_001494"
     CDS             2596..3750
                     /locus_tag="WGR46_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0856409.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWQRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQEKHEEVKKLIYQLIELDFLELL"
     gene            3759..3965
                     /locus_tag="WGR46_001495"
     CDS             3759..3965
                     /locus_tag="WGR46_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856410.1"
                     /translation="MKNWKQFTFFVILVIACYQLLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            4170..4529
                     /locus_tag="WGR46_001496"
     CDS             4170..4529
                     /locus_tag="WGR46_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0856411.1"
                     /translation="MKRKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDSTNYC"
     gene            4590..5306
                     /locus_tag="WGR46_001497"
     CDS             4590..5306
                     /locus_tag="WGR46_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0856412.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYALDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            5371..5826
                     /locus_tag="WGR46_001498"
     CDS             5371..5826
                     /locus_tag="WGR46_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0856413.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYE"
     gene            6549..6851
                     /locus_tag="WGR46_001499"
     CDS             6549..6851
                     /locus_tag="WGR46_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856414.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            7135..7374
                     /locus_tag="WGR46_001500"
     CDS             7135..7374
                     /locus_tag="WGR46_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0856415.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            7435..9180
                     /gene="hrpB"
                     /locus_tag="WGR46_001501"
     CDS             7435..9180
                     /gene="hrpB"
                     /locus_tag="WGR46_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0856416.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEEGK
                     SDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVH
                     KTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQ
                     YQSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAY
                     LNRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQ
                     FFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPR
                     HQFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGF
                     QTANTVYGFNLNYSF"
     gene            9289..12240
                     /locus_tag="WGR46_001502"
                     /pseudo
     CDS             9289..12240
                     /locus_tag="WGR46_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
BASE COUNT         3671 a         2835 c         3132 g         3282 t
ORIGIN      
        1 tttttgacac attttcatga tttcaaatcc cgttattccc aagtggacgg aaacccgata
       61 atgggaagcc gcagagactt atcgggaaaa cggcaagccc tccgccgtca ttcccgcgca
      121 ggcgggaatc tagaaataaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
      181 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
      241 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcacgg caatgatggg
      301 attttaaatt gcaggtattt accgggaaaa acggcaagct ctccgccgtc attcccgcgc
      361 aggcgggaat ctagaaatga aaaaccacgg tgttatcgga aatgattgaa accggagaaa
      421 tgacggaatc gaagcgtgtg gaaatgtgtc ggaaatcgct gaagccggat aaactagatt
      481 cccgcttttg cgggaatgac gggattttag gtttttggtt tgttttcgcg gcaatgacgg
      541 gattttaggt ttttggtttg ttttcacggc aatgatggga ttttaaattg caggtattta
      601 ccgggaaaaa cggcaagccc tccgccgtca ttcccgcgca ggcgggaatc tagaaatgaa
      661 aaaccacggt gttatcggca atgactgaaa ccggagaaat gacgaaattg aagcgtgtgg
      721 aaatgtgtcg gaaatcgctg aagccggata aactatagtg gattaaattt aaatcaggac
      781 aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac gctgtactgg
      841 tttaaattta atccactata gattcccgct tttgcgggaa tgacggtatt ttagattttt
      901 gttttcgcgg aataatgagc caaaagccgt ccgaacccaa aaaaaccgtc gctttcagac
      961 ggcatcaaaa aaccgcaggc acgatgcctg cggtttgccg tttgcgcttc aggggagcag
     1021 ggggattgcg cccaagccgt gtgtttcctt cagtccgaac atgatgttca tattttgcac
     1081 cgcctgaccc gccgcgcctt tgacgaggtt gtcgatgacg gaaaggacga cccacacatc
     1141 ggattgcgcc gcctgttgga tgctgatgcg gcagaggttt gcgccgcgca cgctgcgggt
     1201 ttcgggtgtc gaaccggcgg gcaggatgtc cacgaacagg ctgtcgcggt aatagtcgcg
     1261 caggatggtt tcggggcaga tgccgtctga aaggtggagg taaacggtgg cgtgcatacc
     1321 gcgtatcatc ggcgcgaggt gcggcgtgaa cacgaatcct tcggcgatgc cgtcctgaag
     1381 cccggcgatg gtctgcctga tttcgggcag gtggcggtgt ccagctatgc cgtatgcttt
     1441 gaagttgtcg ccgacttcgc acaacagcga accgacattg cctttcctgc ccgcgccgga
     1501 cacgccggat ttgcagtcgg caatcagcgg cataccgggc ttcagacggc attgccgcaa
     1561 cagcggcacg agcggcaggg atacgcaggt cgggtagcag ccggggttgg cgacgaggcg
     1621 tgcctgtgcg acggcttcgc ggttgagttc gctcaatccg tacacggctt gggaaacgag
     1681 gtcgggggcg gcgtgggtca tgccgtacca gtgttcccag gtcggaatgt cccgtatgcg
     1741 gaagtcggcg gaaaggtcga tgacgcgcac gccctgttca atcaggcgcg gcgcgtcttt
     1801 catggcgatg ccgttgggcg tggcgaagaa gacgatgtcg cattgttcca aacctgcctc
     1861 gtcgggcgtt tggaaggcga ggccgtacac gccgcgcaaa ctcggaaagt aatcggcaac
     1921 tgcagttccc gcttcgctgc ggctggttac ggcggcgact tcgacatcgg gatgggcggc
     1981 aagcaggcgc agcagttcca cgcccgtgta gcccgtcgcg ccgacaatgc cgactttgat
     2041 ttttttactc atggtttttc ctttgtgtgg ttggcgggta tgccgtccga acgctgtccg
     2101 acggcttgtc cgacggggtt ttgattggcg gcggaaggac ggtacacaaa gcgtttgaaa
     2161 agcgtgtcta atttaatggc atccgcctct gtttttaaaa cccagccctg ccgtccggaa
     2221 taaacataac cgtaggcgcg aaagtcggca ggatatttcg gtgcctgccc caaacccaaa
     2281 ccgtatgcgg cgaaagcggt ggtcgtggag aaggaaagca ggaggaggac gagggttttc
     2341 ataaggttcc aaaccgaacg gggcgatgaa gcccgattat agcaaaaggt cgtctgaaaa
     2401 ccgttttcag acgacctttt atttaattac aataaaaatg ttaacaacaa aaaacaaacc
     2461 gcttttttcc gtttggagac aaatttttaa ctaaaattgt caacaattcc ttgacgcaca
     2521 cacaaaaaaa acttcaatat ttgtaaatat atgtaacatt ataaatttta aatattttct
     2581 gagtaagggg aagtaatgag catccatctc gacttcggca ttagtcctaa aacgttccga
     2641 cagacttatc tgtatcaaaa gcccaagctc tttaaaggag cggttcggaa tctcgaatcc
     2701 gcatcttgga aagatatcaa cgagatatac caacgagcag acccaaccgc accgctgttt
     2761 catctgcgta aaaaaggcgc aatcgttcct aaagaagaat acgtcgaaag tttcgacgat
     2821 ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct acgaacatat gaagaatggt
     2881 gcgtcgttag tctataacca tattaacaac gagccgtttt cagaccatat cgcccgtcaa
     2941 gtcgcccgct ttgccggcgc acatactatt gttagtggat atcttgcttt tggcagcgac
     3001 gaatcttata aaaaccattg ggacacccgc gatgtgtatg ccatccagct tttcggcaag
     3061 aaacgttggc aacttactgc ccctgatttc cctatgccat tgtatatgca acagactaaa
     3121 gatactgata tttccattcc tgaacatatc gatatggata ttatccttga agcaggtgat
     3181 gtcctctaca tcccgcgcgg ttggtggcaa agacctatcc cgctcggctg tgaaaccttc
     3241 cacctcgctg tcggtacctt tccgcccaac ggctataatt acctcgagtg gctaatgaag
     3301 aaattcccca ccatagaaag tctgcgccac agtttctcag actgggagca agataggacg
     3361 cgtatcaacg atactgccgc acaaattgct gccatgattg ccgaccccgt caattacgaa
     3421 gccttcagtg aagacttcct cggcaaagaa cgtaccgata ccgcttttca tctcgaacag
     3481 ttcgcgaatc ccaacgctac tccgctttca gacgacgtca ggttgagact aaatgccaat
     3541 aatttggata cgttggaaaa gggatatttg attgggaatg ggatgaagat aagcgtagat
     3601 gagttgggga aaaaagtgtt agaacatatc ggtaagaatg aaccgttatt gttgaaaaat
     3661 ctactggtta acttcaatca ggaaaaacat gaagaagtta agaagttgat ttatcagttg
     3721 atagagttag attttctgga acttttgtga gggattctat gaaaaactgg aagcaattta
     3781 catttttcgt aatattagta atagcttgtt atcaattgct atatttttta tcagatatgt
     3841 ttcttctcga ctacattaac aaatatagtt ggaatttgaa ttttattcag ggtactttga
     3901 attttttttc aatatatctg ccatatgttt ttgttagccg aatttttagg aataccaacc
     3961 aatgaaagac taggctatga aatcaaaatt tgtgtataag ttactggtgc ttgcaaagta
     4021 cctgtatatt ttaatttgta tatcaatacc aatcgttttt atttatgaaa gctaccaagg
     4081 agatgatgtt aattttatga tttatattcg tgcttttatt attttttcca tattgggtgt
     4141 attgactcaa tattctatgt ctaatttttt tgaaaaggaa aaataacatc attgaactat
     4201 ccattgagaa tctggagcta atttatggcg gaggcttagg ggagagaatc cttgaaggag
     4261 gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg tattggtctg ggtgtcggta
     4321 tagtttctgg tggtattcct gccggaccgg ctattattct cggtggcaca gttggtgctg
     4381 ctgttggtgc tgtaactggt atgggtgttg cagcatatga gtattaccaa gaaactcagc
     4441 ccaaagggac agtaacggtc ggtccgattt ctgcttttgc tgtggacaaa tcaggaaacg
     4501 gctacactga cagcacaaat tactgctgat atttctcatc gtccagacaa tctctagggg
     4561 tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc caagcaaccc ttcttccgtc
     4621 ccgaagtcgc cgttgcccgc caaaccagcc tgacgggtaa agtgattctg acacgaccgt
     4681 tgtcattttc cctatggacg acatttgcat cgatatctgc gttattgatt atcctgtttt
     4741 tgatatttgg taactatacg cgaaagacaa cagcggaggg acaaatttta cctgcatcgg
     4801 gcgtaatcag ggtgtatgca ctggatacgg ggacaattac agcgaaattc gtggaagatg
     4861 gagaaaaggt taaggctggc gacaagctat ttgcgctttc gacctcacgt ttcggcgcag
     4921 gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt gaagaaaacg ttggcagaac
     4981 aggaactggg tcgtctgaag ctgatacacg ggaatgaaac gcgcagcctt aaagcaactg
     5041 tcgaacgttt ggaaaaccag gaactccata tttcgcaaca gatagacggt cagaaaaggc
     5101 gcattagact tgcggaagaa atgttgcaga aatatcgttt cctatccgcc aatgatgcag
     5161 tgccaaaaca agaaatgatg aatgtcaagg cagagctttt agagcagaaa gccaaacttg
     5221 atgcctaccg ccgagaagaa gtcgggctgc ttcaggaaat ccgcacgcag aatctgacat
     5281 tggccagcct cccccaagcg gcatgagaca gaacaaagct ggcttgaacg caccattgcc
     5341 gatatttctc aagaagtttt ggattttgaa atgcgctctg aacaaatcat ccgtgcagga
     5401 cggtcgggtt atatagcaat accgaacgtc gaagtcggac agcaggttga tccttccaaa
     5461 ctgctcttga gcattgttcc cgaacgtacc gagctatatg cccatctata tatccccagc
     5521 agtgcagcag gctttatcaa gccgaaagac aaggttgtcc tacgttatca ggcatatccc
     5581 tatcaaaaat tcgggcttgc ttccggcagt gtcgtatcag tagcaaaaac ggcactgggc
     5641 agacaggaat tgtcgggatt gggcatggta tcctccgatt tggcgaagag caacgaacct
     5701 gtttatctcg tgaaaataaa acccgacaaa ccaaccatca ctgcatacgg tgaggaaaaa
     5761 ccgctgcaaa tcggcatgac gttggaagca gacatcctac acgagaaacg gcgattgtac
     5821 gaatgagacc tttgcaataa cataggttac taaaatttta tgctcaatct cattttcaaa
     5881 atgcaaaact tttctgattt ttcctacttt ttgctcaata ttaggaaggt tttaggcaat
     5941 tgaaaatttt ttggcgcatt tttatgcgtc aaatttcgtt aacagactat ttttgcaaag
     6001 gtctcgaatg ggtattggag ccgatttata gtatatcggg caaactgtaa gacttgaggc
     6061 tgaaagataa agaagttgtc tgaaaccttt tcagacgacc agaagacaat atataaaaac
     6121 acaaacttca aaactgcctg aaaccatccc cttttacagg cactccgttt gagacctttg
     6181 caataacata ggttactaaa attttatgct caatctcatt ttcaaaatgc aaaacttttc
     6241 tgatttttcc tactttttgc tcaatattag gaaggtttta ggcaattgaa aattttttgg
     6301 cgcattttta tgcgtcaaat ttcgttaaca gactattttt gcaaaggtct ccgtctgtaa
     6361 aagcaaggat agggcatctg cccttttgat tgtttgatta acgatacaag gagtttcaaa
     6421 atgagagttt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact
     6481 atttgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc actatatgtg
     6541 ttcatgaaat gacttgggtc ggaggctcag gtaatgcgca acaaagttca tattattgcg
     6601 aaatttgcga atctgcaggg cttaacgata cgggaaatcc tgataaatct ttaggattgc
     6661 caaacaatac gttcagtaat ccgcctggtt ggggagctac aatcggagct ttagcaggta
     6721 gccgcatagg gatgcctgaa tttggtacgt ttgcgagcca tgccattgaa aatttcgact
     6781 ggtcatggta tcgacgttat agggaaattg ccgaaacgat tgaacgagaa tattcaggcg
     6841 gtttgcctta atagttgagg aggtcatgat gtttgccaaa cattatcaat tcatcgcact
     6901 cggcatcatg ctgcttcttt atatgttgat tctctatacg accgattttt ccaatctgac
     6961 gtattggatg ctgtttttta tctgttttat tacaggaaaa atattagctc gtttgttaga
     7021 gaaaagcttt aaataaaata gcagctagtc gcaaaaggtc gtctgaaacc ttttcaggcg
     7081 gcctttctaa aatacatcca acttcctaat ccctattttt caaaaaggaa atctatgccc
     7141 catctgcaaa acctgtcttt gggcttaaag aaaaagctgc ctgttatcct gcaaacagaa
     7201 atatcagaat gcggcttggc atgtctggcg gctgtggcgg gatttcatgg tttccatacg
     7261 aatttacgcg cactgcgttc aaaatactgt ccgagacctt tgcaaaattc cccaaaatcc
     7321 cctaaatgtc ttggtgggaa ttttggggaa ttttgcaaag gtctcattct ataactgtaa
     7381 atacttttaa atttatgaca aaatagtaaa tattgctaaa ataatattga tgtcatgaaa
     7441 ttttttcctg ctccatgtct gttggttatc ctggctgtca taccccttaa aaccttagct
     7501 gccgatgaaa acgatgcaga acttatccgt tccatgcagc gtcagcagca catagatgct
     7561 gaattgttaa ctgatgcaaa tgtccgtttc gagcaaccat tggagaagaa caattatgtc
     7621 ctgagtgaag atgaaacacc gtgtactcgg gtaaattaca ttagtttaga tgataagacg
     7681 gtgcgcaaat tttcttttct tccttctgtg ctcatgaaag aaacagcttt taaaactggg
     7741 atgtgtttag gttccaataa tttgagcagg ctacaaaaag ctgcgcaaca gatactgatc
     7801 gtgcgtggct acctcacttc ccaagctatt atccaaccac agaatatgga ttcgggaatt
     7861 ctgaaattac gggtatcagc aggcgaaata ggggatatcc gctatgaaga aaaacgggat
     7921 gggaagtctg ccgagggcag tattagtgca ttcaataaca aatttccctt atataggaac
     7981 aaaattctca atcttcgcga tgtagagcag ggcttggaaa acctgcgtcg tttgtcgagt
     8041 gttaaaacag atattcagat tataccgtcc gaagaagaag aaggcaaaag cgatttacag
     8101 atcaaatggc agcagaataa acccatacgg ttcagtatcg gtatagatga tgcgggcggc
     8161 aaaacgaccg gcaaatatca aggaaatgtc gctttatcgt tcgataaccc tttgggctta
     8221 agcgatttgt tttatgtttc atatggacgc ggtttggtgc acaaaacgga cttgactgat
     8281 gccaccggta cggaaactga aagcggatcc agaagttaca gcgtgcatta ttcggtgccc
     8341 gtaaaaaaat ggctgttttc ttttaatcac aatggacatc gttaccacga agcaaccgaa
     8401 ggctattccg tcaattacga ttacaacggc aaacaatatc agagcagcct ggccgccgag
     8461 cgcatgcttt ggcgtaacag gtttcataaa acttcagtcg gaatgaaatt atggacacgc
     8521 caaacctata aatacatcga cgatgccgaa atcgaagtgc aacgccgccg ctctgcaggc
     8581 tgggaagccg aattgcgcca ccgtgcttac ctcaaccgtt ggcagcttga cggcaagttg
     8641 tcttacaaac gcgggaccgg catgcgccaa agtatgcccg cacctgaaga aaacggcggc
     8701 gatattcttc caggtacatc tcgtatgaaa atcattactg ccggtttgga cgcagccgcc
     8761 ccatttattt taggcaaaca gcagtttttc tacgcaaccg ccattcaagc tcaatggaac
     8821 aaaacgcctt tggttgccca agacaagttg tctatcggca gccgctacac cgttcgagga
     8881 tttgacggtg aacagagcct ttccggagag cgaggtttct actggcagaa cactttgggc
     8941 tggcatttcc atccgcgcca tcaattctat ctcggcgcgg actatggtcg ggtatcgggc
     9001 gaaagtgccc aatatgcctc gggcaagcgg ctggcgggtg cagtggccgg cttcagaggc
     9061 agccataaag taggcggtat atttgcttat gacttatttg ccggcaagcc gctgcacaag
     9121 cccgaaggtt ttcagacggc caacaccgtt tacggcttca atttgaatta cagtttctaa
     9181 cctctgaatt ttttactgat atttagacgg tctttcctta tcctcagacc gtcaaacttt
     9241 acctacgtac ttggcgcgca gtacgttcat cttcaaaatg gaatagacat gaataaaggt
     9301 ttacatcgca ttatctttag taaaaagcac agcaccatgg ttgcagtagc cgaaactgcc
     9361 aacagccagg gcaaaggtaa acaggcaggc agttcggttt ctgtttcact gaaaacttca
     9421 ggcgaccttt gcggcaaact caaaaccacc cttaaaacct tggtctgctc tttggtttcc
     9481 ctgagtatgg tattgcctgc ccatgcccaa atcaccaccg acaaatcagc acctaaaaac
     9541 cagcaggtcg ttatccttaa aaccaacact ggtgccccct tggtgaatat tcaaactccg
     9601 aatggacgcg gattgagcca caaccgctat acgcagtttg atgttgacaa caaaggggca
     9661 gtgttaaaca acgaccgtaa caataatccg tttctggtca aaggcagtgc gcaattgatt
     9721 ttgaacgagg tacgcggtac ggctagcaaa ctcaacggca tcgttaccgt aggcggtcaa
     9781 aaggccgacg tgattattgc caaccccaac ggcattaccg ttaatggcgg cggctttaaa
     9841 aatgtcggtc ggggcatctt aaccaccggt gcgccccaaa tcggcaaaga cggtgcactg
     9901 acaggatttg atgtgcgtca aggcacattg accgtaggag cagcaggttg gaatgataaa
     9961 ggcggagccg actacaccgg ggtacttgct cgtgcagttg ctttgcaggg gaaattacag
    10021 ggtaaaaaac tggcggtttc taccggttct cagaaagtag attacgccag cggcgaaatc
    10081 agtgcaggta cggcagcggg tacgaaaccg actattgccc ttgatactgc cgcactgggc
    10141 ggtatgtacg cagacagcat cacactgatt gccaatgaaa aaggcgtagg cgtcaaaaat
    10201 gccggcacac tcgaagcggc caagcaattg attgtgactt cgtcaggccg cattgaaaac
    10261 agcggccgca tcgccaccac tgccgacagc actgaagcat caccgactta tctctccatc
    10321 gaaaccaccg aaacaggcgc agcaggcgca tttatctcca atggtggtcg gatcgagagc
    10381 aaaggcttat tggttattga gacgggagaa aatatcagct tgcgtaacgg agccgtggtg
    10441 cagaataacg gcagtcgccc agctaccacg gtattaaatg ctggtcataa tttggtgatt
    10501 gagagtaaaa ctaatgtgaa caatgccaaa ggctcggcta ctctgtcggc cggcggtcgt
    10561 actacgatca atgatgctac tattcaagcg ggcagttccg tgtacagctc caccaaaggc
    10621 gatactgaat tgggtgaaaa tacccgtatt attgctgaaa acgtaaccgt attgtccaac
    10681 ggcagtattg gcagtgctgc tgtaattgag gctaaagaca ctgcacacat tgaagcgggt
    10741 aaaccgcttt ctttagagac ttcaaacgtt gcttctaaca tccgtttgaa caacggtagc
    10801 attaaaggcg gaaagcaggt ggtattgatg gctgatgacg acattcaggc aaaagcctcc
    10861 cacctgaatg cgtccggcaa tctgtatatt catgcaggta aggatctgga tttgaatgcc
    10921 gataaagact tatcaacaca aagtatcagc ctgagggcag acaacaccgc cctcatcagc
    10981 agcaacggta acaccttgac tgcagaaaag aatctggata ttcaggcagg tagcttgagt
    11041 gtgcgtcaaa gtaatctgca atccagtggc ggtaatgtgc agatgagcgc gactaaaggc
    11101 aacatcagct tgaatcagag ttggataaat gccagtcaga atattgatac ggcagcactt
    11161 caaggcaata ttatttcaga cggtctgact gctgttgccg aagttggacg cgtatctctt
    11221 cttgccaacg gcaatgttga tttcaacggt ctaaatacct tgattgcgga aggagacatt
    11281 aatgctggtt cagtcggtaa aggccgtctg aaaatggaca ataccgatat ttatgcttct
    11341 gcaggcgatg tgaaactggt tgccggaggt caattagatt taggcaacgg caccgttaac
    11401 ggcggtcata tcagcttaga cagcaataaa ggcagcatgg tggtgcagaa tgtacacctg
    11461 aatgcccgcg catcactgaa agtggatgcg gatcaaactt taaccattaa taacagcaag
    11521 ctcaattccg atcacaatac ccagattaat acaaatcatg gtcatatgac gcttaatcag
    11581 cttgatgctc attcacgtcg tcatatgagt atcagtgcac agggtaaagg caaaggaaaa
    11641 gacagcggtc aaattttaca aaacgaccag caaaacagta aaagtacttt ggcggcagat
    11701 ggtgtattgt cattgaacag cagcgcatta caggttttgg acaatactac cctgcgcggt
    11761 ggcgcgataa acatcaaagc tggcggaggc atcatcaaac gggggcatat cgattgggaa
    11821 acccaagata cggcaactat gcgttcagcg gaactgaaac cactgtccgg tatgatgtcg
    11881 atagaatcgg gcggtgataa ccctttaacc gttgaacccg gtaaccgtat cgtttctgca
    11941 ggtgatttgg ctgtgaaaca caacggcaca ttccaaatca gtgccagagc aggaaataac
    12001 ggcaatccga gtgcgcaaac agccagcgtt tcagctaaag gcaatatcgg gattgtggcg
    12061 ggagaggtgg atattgatgc agcaaatatt gccgcaggga aagatttggc tttagtggca
    12121 actaaaggga atatttcact aaacagtatt aggaatacgt ttagtaacta tcaattgaaa
    12181 acagataaac acaatatcac acagcaactt actgatgtag agcaggagct tagtaagtta
    12241 acgagcgatc ctaaatatcg taaagcacag gatcttccac aaatgttgag gcgtaaatat
    12301 aagaggcgtg acaaagtatt cggtgatagt gaggccagat tacgtggttt acgagcaaag
    12361 ataaatgctg ctgatgaggc ttgggcagaa cgccaatccc cagtaaaagc gttacttgaa
    12421 cgtaaacaat tgttacaaca agccctttta acagtttccc aaccgggtag tggtcacgaa
    12481 aaccaaggta gcacccttag cggtcaaaat atcaaactgc tcgctgcagg tggtatccgt
    12541 atccagggct ccaaagttgc tgccacccaa caggcaaata tccaagctgc aggattttta
    12601 cctgcaccag ccgcagagga actgcaagaa ggtcgacttc aatcagccat tgacatcagc
    12661 ggtgtacttg atacttttga atatggtcag caaggcagcg ataaatacgg ctatgccatt
    12721 ttcagcaggc catctgaaat ttcaggaaaa acaggtgtta ccctctctgc accaaatgcc
    12781 aatgagaaca gccgcatcag tctgagtgcg gcaaacatcg aagctgaaaa cggcaaaatc
    12841 aaaattcaat cttacggtga ccaatattac tatgcgagac agagcgaact ctataccttt
    12901 gaacgccgca gctacaaaac
//
LOCUS       DAWFKN010000057        12834 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFKN010000057 DAWFKN010000000
VERSION     DAWFKN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.057"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(963..1631)
                     /locus_tag="WGR46_001503"
     CDS             complement(963..1631)
                     /locus_tag="WGR46_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0856417.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGR46_001504"
     CDS             complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGR46_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0856418.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2401..2979)
                     /locus_tag="WGR46_001505"
     CDS             complement(2401..2979)
                     /locus_tag="WGR46_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0856419.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            complement(3201..3413)
                     /locus_tag="WGR46_001506"
     CDS             complement(3201..3413)
                     /locus_tag="WGR46_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856420.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(3580..4293)
                     /locus_tag="WGR46_001507"
     CDS             complement(3580..4293)
                     /locus_tag="WGR46_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0856421.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFAVESLTEL
                     SNDDLQKTYAAVSTKKRKK"
     gene            4526..4777
                     /locus_tag="WGR46_001508"
     CDS             4526..4777
                     /locus_tag="WGR46_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856422.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4779..6756
                     /locus_tag="WGR46_001509"
                     /pseudo
     CDS             4779..6756
                     /locus_tag="WGR46_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6823..6900)
                     /locus_tag="WGR46_001510"
                     /pseudo
     CDS             complement(6823..6900)
                     /locus_tag="WGR46_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6971..7885
                     /locus_tag="WGR46_001511"
     CDS             6971..7885
                     /locus_tag="WGR46_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0856423.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMQDSKARREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDEELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            7976..8242
                     /locus_tag="WGR46_001512"
     CDS             7976..8242
                     /locus_tag="WGR46_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856424.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            8351..8524
                     /locus_tag="WGR46_001513"
     CDS             8351..8524
                     /locus_tag="WGR46_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856425.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            8517..8684
                     /locus_tag="WGR46_001514"
     CDS             8517..8684
                     /locus_tag="WGR46_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856426.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8748..8975
                     /locus_tag="WGR46_001515"
     CDS             8748..8975
                     /locus_tag="WGR46_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856427.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            8990..9508
                     /locus_tag="WGR46_001516"
     CDS             8990..9508
                     /locus_tag="WGR46_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0856428.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(9668..9883)
                     /locus_tag="WGR46_001517"
     CDS             complement(9668..9883)
                     /locus_tag="WGR46_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856429.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(9887..10485)
                     /locus_tag="WGR46_001518"
                     /pseudo
     CDS             complement(9887..10485)
                     /locus_tag="WGR46_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(10617..10817)
                     /locus_tag="WGR46_001519"
     CDS             complement(10617..10817)
                     /locus_tag="WGR46_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856430.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(11018..11203)
                     /locus_tag="WGR46_001520"
     CDS             complement(11018..11203)
                     /locus_tag="WGR46_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856431.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(11476..11643)
                     /locus_tag="WGR46_001521"
     CDS             complement(11476..11643)
                     /locus_tag="WGR46_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856432.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            11880..12122
                     /locus_tag="WGR46_001522"
     CDS             11880..12122
                     /locus_tag="WGR46_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856433.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            12166..12357
                     /locus_tag="WGR46_001523"
     CDS             12166..12357
                     /locus_tag="WGR46_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856434.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            12354..12770
                     /locus_tag="WGR46_001524"
     CDS             12354..12770
                     /locus_tag="WGR46_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856435.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         3428 a         2575 c         3377 g         3454 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 aggctccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatt actttcctag agtaagggta
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtaaat gcagagtggg tggaaacact cactttatgg tttgctacgc tctgttcaat
      661 tagcaacccg ataacccaat atagataata gggtaattaa tctaatctaa tttgtcagca
      721 tccgttaatt tattgcaaaa taaagtattg aattatgtcg ggtgcaaatg acgaaatata
      781 agtttccgtg cggacggatc aagattccca ctttcgtggg aatgacggtg gaaagattgt
      841 tgtttttccc gatgaattcc tgtgtttttt gtttttccgg ataaattcct gtggctttga
      901 gttttttgga tttcagcctc aatgccgtct gaacgccgaa tcgggcttca gacggcattg
      961 cgtcatttga aattcaaaac cggccagcct ttttctttgg cttctttttc cagctcggca
     1021 tcggggttga cggcgacggg ttcgtcgacg aggcgcagca gcggcaggtc gtttttggag
     1081 tcgctgtaaa aataggtttt gccgtagctt tcgagcgttt cgccgcgttc ggcgagccat
     1141 tggttcaggc gggtgatttt gccttctttg aggctgggcg tgccgatgta attgccggtg
     1201 tagcggccgt cagaaccggt ttcgagttgt gtgccgatga tgttggtgat gccgaaaagg
     1261 tggcagacgg gggtgatgat gaactcgttg gttgaggaaa tcacaagggt ttcgtcgcct
     1321 gccatttggt ggctctgcac cagcatacgc tgcataggcg agatgtgggg gatgatgtat
     1381 tccgccataa attcgcggtg aaactctgcc agctcttctt tgctgtaacg agcgagcggg
     1441 gcaaggtgga atttgaggaa tgcgtcgatg tcgaggcaac cgttttggta gtcgcggtag
     1501 aatttttcgt tttgcgcttc ggtttcggca gcgtcaacca agcctttttt gatgaggtat
     1561 tgcggccagg cgtggtcgga atcggtgttg atgagggtgt tgtcgaggtc gaagatggcg
     1621 aggtttttca ttgggtttcc tgttgtttca aaagctggcg caaaagcggc agggtgatgc
     1681 gtttgcccat tgtgacggcg tagttgtcca gcgtgtcgag catcatcatc aggctgtcca
     1741 tatcgcgccg ccagtgtttg agcaggtatt cgaaaatttc ggaatcgacg gttacttggc
     1801 gtgccgccgc catactggcg agcgcgtcga ttttttcttg gtcggttaag ggcttgactt
     1861 cgtaaacgag gcagtacgcc atacgcgtcc gcaaatcttc gcggatgacg agctgctggg
     1921 gcgtgtattc cgaaccgagc agcaaaaagc ctttgccgct gttgcggaag cggttgaaga
     1981 tggaaaaaag cagggcttgt tcttcgttgc ccagtttttc gacttgatcg acggcgaggt
     2041 attccgcctc gaacgcggca tcggtcagcg gcatggaggc ggcatcgata taggcggcgt
     2101 ttttgccggc ttcgagcgcc tgtgcgaccc acgcctgcaa aagatggctt ttgcccgcac
     2161 cttcttcacc ccagacatag ataaactgtc cgtgtttgtg tcggaggaca tagaccagtt
     2221 ccgcgttttc cgtgccgagg aatttgtcga aactcggata gtcgtgtgcg gcaaagtcga
     2281 aaataagctg gttcacggtt cggcattccg aggggtggta aacgggttta ttgtacgttg
     2341 ttttcgcgtg ctttatcaat ttgaacgatg ccgtctgaaa acggcttcag acggcatcgt
     2401 tcaaccgcag gcaacgttgc cgacatcgag gcgcatattg tggaacgcgt tgagcgtgct
     2461 tcggtgtccg atgctgatga tgatgctgtc gggcagtttt tgctttaagg cgcggtagag
     2521 cgcggcttcg gccggttcgt ccaaggcggc ggtggcttcg tcgagcagga cgattttggg
     2581 cttggaaagc agggcgcgga cgaaggcgac gcgttgcagt tcgcccaggg agagtttgtg
     2641 ctgccagtcg tctgttttgt ccaatttgtc aatcagataa cccaagcggc aggtgttcat
     2701 ggcttcgatt agctccggat gctgtttgtc gatgtcggga taacaaaccg cgtcgcgcag
     2761 gctgccctgt gccgtgtacg ggcgttgcgg caggaagagg atgtcttgat gcggcggacg
     2821 gctgactttg ccgctgctgc cgaacggcca aagcccagcc agcgcgcgca gcaacgaagt
     2881 tttgccgcaa ccgctcgggc cgcgtatcag cagggaatcg ccgcttttga gtttgatgtt
     2941 gatgccgctc aacaggattt cgccgttgtg gcggaacagg gcgacgtttt cctgtggggg
     3001 actgttagtt tttgcacaag gaacaaatag agtaaaaaaa cgctgaaatc ttcggaagac
     3061 gtggatttcg gcgttttttt gtatccggaa aagttacgcc agctttttca caaaaccgcg
     3121 ccggaatgcg cggttttctg tttaaagctg acgagattag ggaattttta aaactgtttt
     3181 aagaggtttt taaaatggat ttaatcaata ctccggccat accactcaac acggcctatg
     3241 atggcgatgt cgtcttgggt attactcaaa tctatttcaa acggtgcgta acgtggattt
     3301 tcagacgtta caagcagttt gcccggtata cgttgcacac gtttgacaaa gaggtcattg
     3361 cctatacgca agacatatag gccgtcgcgc ggctcggttt cggcgtggtt gattagaatg
     3421 tttataaaag gctttaacta gaaactgaac atggaacttg tataatagtt ccatgtttga
     3481 aaaaatcttt taatatcata catctaaaaa aacaaaacat ctgaagacca taagctaaac
     3541 atgaaacttg aatttaaagg cggagtttca tgtttaagcc tattttttcc gttttttagt
     3601 cgatactgct gcatatgttt tttgcaaatc gtcattactt aattcagtca ggctttctac
     3661 tgcgaaattt ttttcgaggt aacttttcaa ccaatcttca agctcaagtt gttttacatt
     3721 taatttgata taagcgtatt tcttttttcg ccagtcggga tcgttgttct ttgatgttgc
     3781 tgtattgctc aacctaccca gccattttct gagataagtt tctgctttgt cgtaatcgct
     3841 caatgcgatt agtttgtaac ttggaacttt acagtgagca ttcagactag cccaaattgc
     3901 acggattgat tttggcgacc gtttggcaat ctcttctgct gcggcaactt gtttaacaag
     3961 ttgttgcagc tttgaagctt gctcaagcgt tatatgttcc ttgccgggtt ttgaatcgac
     4021 acgggttctg atgacgtgat tttgggtatt aatttgatga acaaccgaac cagatgaggc
     4081 gataacagcg ttactgacgt tgccgttaat ctgattctca acgtttgatt tgccaatagc
     4141 gtcgtccaat tttttattaa ggtcgtctga aacagcattt aaatttgccg aatgaatgcc
     4201 tgtaaagaca aattgaatat ccacccctaa ttggacagca gcaagtaaaa agtccgcagg
     4261 aatgttgacc tgcccgcttt catacagtct caaactttct gcactgcatc cagtttgctc
     4321 agcaaaattc tttcgagagt atccaagtcg gcttctctct ttaactaaat tcaatgctaa
     4381 atcaatgcgt tccataaaac accgcaaaat agtacccaat atatttggta taccaaatat
     4441 attgggtata tcatccaggt aacagttaag gcaggcatta aagcctgtaa ggtaagtaag
     4501 taatggattc tatcatggag gcattatgca agcgaacgaa atctataaat ccttagaaga
     4561 aaaaaatata tcggcaagaa tgttggcttc ggctttaggg gtaactaacc aatctgtttc
     4621 tgaggtcata agaaatggac gaggaagcaa aagaattgcg gaggcaatcg cgaaagtgat
     4681 tcaaaaagat ttagttgaag tttttccgca ttacaagaaa acagattgtc gagacaaaaa
     4741 gatagctgaa ttgaaagaga tgctcggagt gggttgaaat ggaatatatg gatctcaaaa
     4801 gcctgcttaa ttttgggttg ccaaatatgc caagaacagt aatagggctg agaaaaaaaa
     4861 gcgcaaaggg atggttggca aacgagaagg cgtcaaggta agggtggtgg tgttgaatat
     4921 gccctaccta ttgaaatccg agcagccatc atgaaacggc agtcggacga gctggcggaa
     4981 aagatgccga aaatgttgcc ccaagtcaga ccggggacgg cgatgtcggc tcaggcactg
     5041 gctgaagcgg ccaagctgtt gaacgagaaa caacggtcgg tggcggatgc gcgatgtgcg
     5101 gtggtggcgg cggtattggg gattaaatac caatacggtt gctctgcaaa ggctgcggtg
     5161 gctcagtttt tgggcttgct ggcagaaagt aaattggacg cggtcacgct tgggaacttg
     5221 gaaaaggcca atgaccgcag ccggacggca aaggttggcg aacgtacttt agacggctgg
     5281 atttctgctt atttgaaagc ggaaaacgcg acggagcggt tggttgcttt ggctccgaag
     5341 acgacgaagg cggtcaagcc gattgagagc tacggttggt tgccgatgtt tatgcagttt
     5401 cacaatatcc cttcggctcc gaaactggcg cacagctacc gccggtttgt gcagtgggcg
     5461 gaagcggaaa atatgccggt caatgatgtg cctaacttga gtatggtgcg gcgcgtttgg
     5521 gacaagctcc cgctgattat gcaggagcgc ggcaggaaaa cgggggcggc ttataaatcg
     5581 ctgttgcctt atgtgaaacg tgattggggg gctttgaagc cgaacgatgt ttggatcggc
     5641 gacggccata gctttaaggc aaaggtggcg catccggtac acggcaggcc gtttaagccg
     5701 gaagtgacgg tgattattga tggttgtacg cggtttgtgg tcggtttttc ggtgtcgttg
     5761 gctgaaagtt gtgtggcggt atcggacgct ctgcgtatcg gggtcaagca ctttggtttg
     5821 ccgattatct actactcgga taacggcggc ggccaaaccg gcaagacgat agaccatgaa
     5881 atcacgggta ttacgtcccg attgggtatc cgccatgaaa cgggtatcgc gggcaacccg
     5941 caagggcgcg gcatcatcga acgatggtgg aaagacaatc tgattgagat ggcgcgacag
     6001 tatgagacgt ttgcgggcgc ggggatggac agcagcacga agaacctgat gtaccgcaag
     6061 atggaaagtg cttttaatgc tttggaaaaa ggcaaggagt tgacggtaga acagcaaaaa
     6121 tatttgaaaa aactgccgag ctggtcgcgt tttatcgcgg atgtggccaa gtgtatcgac
     6181 gaatacaaca accgcccgca cggcgagctg ccccgacatc ctgacggcgg gcattattcg
     6241 ccgaaggctt atcgggaaat gaggctggaa caggacggta tcgcgccgga tatgttgtcg
     6301 gcggaagagc tggcgacgat gtttatgccg caggaggtgc gaaaagtaca gcgcggttgg
     6361 ctggatttgt tcaacaactc ttatttctca accgagctgg cggagtatca caaggacgag
     6421 gtacgggtca gctacgattt gagcgatgcg tcggcggtca atgtgtttga tatggacggc
     6481 aagtttatca ctaaggcgca ggccaacggc aatacccgcg aggctttccc gacggctcgt
     6541 atcgaccaac tggcggaaaa ccgccgaaaa ggcaaaatca agcgggcgga aaatgcaatc
     6601 aagctcgcaa atgcggaagt caatccggca ctggaacagg ctgcggtttg ggacgagctg
     6661 ggacatttgg gcggaaacgt catcgaggcg gagtatgcgg tattgccgaa aacgggcacg
     6721 gacgacggga ttttcttgtt tgaggcggat atgtaaagga aaacatgatg gaaaaacaga
     6781 aaaatgcggc gttttcggcc gagcttgttg aaaaattgaa actcaagcga gcccttgggc
     6841 ggattcaacg atttcaagca aagattcaag gtgttcccgc tgaacggaat caggctcaaa
     6901 tttctaaatc gggaaactct ttcatttcag acggccttta aaggttgttt aaaactcaag
     6961 gatattaaaa atgaaacaaa ttaatcaagc attgcaacaa aaactggttg aatttaaaga
     7021 aaaatcgggt atgaaccaaa cccaactggc acgcggtatc gatacttcac cggcctccat
     7081 cagtatgtat ctgaacggca cttatgccga taaaggcggc aattatgaaa ccatcgagac
     7141 gaaaatcgag gcgtttttgg agatgcagga cagtaaagcg cgacgtgaag agctggtgtt
     7201 gggatttgta tcgactaaga cgacacgccg tattgcggaa gcgatgcgcg atgcgcacga
     7261 aggcggcgaa atagtggtga tctacggtca ggccggtttg ggcaagactc aggcggtcaa
     7321 aaactactgc gagaaaaacc ctgcggccat cttgattgag gctaatccga gctttacggc
     7381 tttggtcctg atgcgcaagt tggcgacggc ggcgaaggta tcggcgatgg gcagcctgaa
     7441 tgatttgttt gaatcggtat cggacagatt gcgcgattcg ggccgtctga ttgtggtcga
     7501 tgaagcggaa aacctgccgt tacgcgccct tgagattatc cgccgactgc atgatgaaac
     7561 gggctgcggt ttggtgttga gcggtatgcc ccgattggtg gcgaatttgc gcggtaagca
     7621 tggcgagctg gtacagcttt acagccgcgt gtcgcttccg ctgaatttgg gcgaatcttt
     7681 gccggatgaa gaactctttg agattgcgaa agcggctttg cctgatgcgg acgaggagac
     7741 gctcttggaa ctggttaaac atagtaacgg caatacgcgc cggatgagca aattgatgcg
     7801 cggcgcggta cgcacggcga acaagaacgg tatcaagatg caggccggta tcgttaaaaa
     7861 atacagctcc ctgattatcc gatagggccg tctgaaacgg taagtctctg acagggctat
     7921 atattttttt accctatgat tttaataagt tgttgtttta aaaggaaaac gcaaaatgca
     7981 agttttgaag aaagttgatt ggaagatgtt tgtggcacgc tctttttggc ggtgtgtgcc
     8041 ggttggtttg acggcgggcg tgtggtgttt tgtggcgggg atggcgttgc attcctgcgc
     8101 gaaagaacct gaaccggttg cgaaagagct gacgaaggtc gagaagatgg aaagacaggc
     8161 ggatttggag gtcaaggtat gggaagagca atacgagtca atgagcgtcg aggaaaaaat
     8221 gaaagggatt gtttatgagt gataagccaa ttttattgag tcctgcggcg aaaaaagagg
     8281 ctttggatcg ggcggtaaag gaaatccgtg caaaatatgg cgataaggcg attgtgaaag
     8341 gatgtgtgaa atgagtttcg gacgacgtaa tacggattgg caggcttggg gacaacaccg
     8401 caggcgtgcg acggcgcgaa tggcgcaaaa aagccgggag cgcgaaatcg aggaatatca
     8461 ggcgcagttt gaagataagg acggcaaagg tcgtctgaaa acgggaggaa acaaagatga
     8521 atgaaaaaga tttgatcgaa tggctcgaag accgtggcga gctgatggtc atgaagaagg
     8581 acggcgaagg ctttgtgatt gcagcgcgtg cgccggacgg gatgtggaag actgccgagg
     8641 cggaaacttt ggctcaggcg ataactttat gggaggaagt gtgatgttta agtttttgag
     8701 aaaccgcgcg aaagttgaag caattttaaa caagaaagga aagtgaaatg aaagtgtatg
     8761 tttttaaaat tagtaatgaa aacggtaagt taaagataga actccccgaa atcccaatgg
     8821 gtaagcaaat tgacgaagtt gatttgattg ctgggctaac cacggaattt attgcaagta
     8881 tgttacgtga tgcccaaaaa gatcgtcgca aattcgtaat cgacgcatca aatcaattgg
     8941 ctgcaatcca gacatatcaa aaaatcttca attaaggaag gaagagaaaa tggctaaaac
     9001 ccgaatcaaa cagcccgcca tcgaagcggc acaagacaaa acggaagtta ctgcatttat
     9061 ccgccaaatc ggcgacttgc agcgcgaagt caaacgcctg gaaaccgaag ccggagacaa
     9121 aaaagcggct atcgaagaag aatatgccgc caaagccgcg ccgatgtgtg ccgaaatcat
     9181 gagcctgacc gaacgtgtgg ccgcctactg cgaagcgcat aaggacgagc tgacggaaaa
     9241 tggtaaaacc aaaaccgtgg actttactac cggcctgatt aaatggcgca ttcgtccgcc
     9301 atccgtcaag gtaacgggcg tggccgccgt cttggcgtgg ctctcggaaa aatccgcctt
     9361 tgccgagttt gtccgcacta aaaaggaaat cgacaaagac gccatcctga atcaaaaaga
     9421 gcgtttttca gacggccaag tgccggggat taagattgtg agcggcgtgg aggattttgt
     9481 gattgagccg agcgaacaaa ttctataaaa tcaacagcct gatttcaatt cagtgtgttg
     9541 aaacgtgatt gagcctacgg agcaggagct tgcctagccg gttcaaatag gctttaaccc
     9601 atgattaaag gccgtctgaa atggggttta aaacctgttt cagacggcct tttttcatac
     9661 cctagattca ggctgctttc tcttcctgct cgtacacggc gttgtagaaa gcggcggaca
     9721 gcttgtcggc tttctcggag gcggtttcaa tcacggcaga caaaccgtct tcgattcctt
     9781 ccatgtccat atccaaaact ttcagatggt tgagcgtgaa caccagcaga ttcagggctt
     9841 tgaggctgtc ttggtcgaaa gtcagagtat aggtggtatt catggcttaa ccctccaatc
     9901 ccaaagactg ttgttgcacc accattttgg gcttggggac atattccaag aaacccaaat
     9961 cgttgagttt tttgagacgg taggaaaccg cgccggggtt catgtccaag agtttgccta
    10021 tttcggtcag gttcaagccc atactgcggt agcgcagcag cgcgagcatt tcgggcgcgg
    10081 cttggaaata ggcgtcttcc aatgcatcga tgcggtatag cacggcatca ggcagggctt
    10141 ttgcctgttt ctccatttcg ataaaatatc ggcgggcttg gcgtcctttg tcgttgcgct
    10201 ccaccatgca cagctctttt gccatgtcga gggataaata atacccctga acagttttat
    10261 cacgctgtcc aaagaaacct gcttcggtgc ggacaatttt gtccacgccg ataaaatcaa
    10321 gggcttgtgt aaatccgtat tcttcaatgc gacgctgaat ccatttagtt ccacacctaa
    10381 gaacttatgc aggtcatgag catcaaccag tgcttgtgtt tgattgtcca attgacctga
    10441 aacagttggg atgagttggg tattcatggt aaaattatct ttcatttcaa ggttgagatg
    10501 acaaggaaaa gaacgctcta acgttgcttt tccactatcc ccgataagct gtcacttatc
    10561 ggggatttcc atatcttgtg ttgcacaaga tatggaaatt ttaaattatg gtaattctat
    10621 ttgtcaatat tcttttttgc tttctttttg aatttttccg attccatatc tcctctaatt
    10681 ttcgatttta cccagtttga aaaatctaaa ttttcagaaa atttaattaa atcaagttct
    10741 ttttcggaat tgaacgatac tgttttcact actcgcttga gtgcgtattt ttcttgagcc
    10801 tttttaatcg agtccataat cttcctttaa ataggtttta aaacacgatt atggtaatac
    10861 taaaaaccat tttaatcaag atgtattaca acaaaaagcc gtctgaaatg gggtttaaaa
    10921 cctgtttcag acggcctttt ttcatgccct acataaagag agttgctgct ctgctttcca
    10981 cctgttccgc cgatgttttc ccatcggcgg aattttctta tttcattttt tccttcatga
    11041 ctgatttaac ccagtttgaa aagtcgggaa tttctttttg cataaaatcc accaactctt
    11101 tctctctttc caaatggaaa gaaactgttt tcatcagccg cttttggtca tactttcgct
    11161 gatattcagc ttgttttgct tttagcgttt tttcatcagg cataggcaat ccttgatttt
    11221 taaaatcgtt gtagtaaaat ggcttgcaga aaggttggcg aaaggggcgc ggtgttctgc
    11281 caccccttgt gtttaatcaa cttgcttagt aagccggact acagcttaaa agcagaatga
    11341 tgaaaaaaac aactttcgcc agttttgtca tttccacaag cctcctttct atccgataag
    11401 cccccgaagt tgccgcttcg gggctttgct ttatcagata ggcgtattat ctagtatttc
    11461 tagaatttag tcaagctatt ttttgatttt ttctttgatg attgatttaa cccattggga
    11521 aaaatcgata ttttgggcat attccaaaag ttctttttcc gtttcggcat tgaacgagac
    11581 tttcttaatc acccgtttct gctcgtaacg ctttcggttt tctgccagtt tttcatcaac
    11641 cataggcaaa ctccttgatt ttttaaaccg tattttgtaa gattgggact ggggacagcg
    11701 gcgaccgccc ccagctggtc agtctttagt aggcattagc acttgccaac atgaaaactg
    11761 cgaccaagat gagaatttga atagtgcgtt tattcatctt ctttctccca gttgagcccc
    11821 gcttcggtgg ggcttttccc gtatcggccc tcactacgcc gatgaattga attataaacg
    11881 tggacacgtt aaaagtcaac cctttcccca acaaaatcaa agaaaaatgc cgtctgaaaa
    11941 cagctcgaat gctgtttcag acggcatttt ttgttgtcgg gcatcaatgc cctgccattt
    12001 gtgcggcgtt gcggttatca agatatggta ttttagtgtg tatttttaga agaaatttac
    12061 catatatagt attaaaatat tatacggttg ttttatatgg atttgatttt cttgaaaaat
    12121 aaatcccata tgctaatatt tgcactattt gggcgaggtt gaaaaatgaa taccgtcaaa
    12181 gaatatacgg cagtccgcga acggctactc aatgctgccg attatttgga agaagtccgt
    12241 aaagaccgta agacgggcaa tattgcgtcc gtggaatttg tgccgccgaa aataggggct
    12301 cggggttacg gcaaattcaa agtccgttac aaaaccttag tggcggttga tttatgagcg
    12361 gggaaaaatc cgagaatctg ccggccgatg agcagcagaa aatgcttcag gagtttttag
    12421 aacttcaaaa acaggatatt gcagtcaaac agaaggagtt ggaaagccgt aaggacgaaa
    12481 tccaatctca cgaacgcatt gcactggcta ccatcgaagc gcaaaaacag ggtgaacagc
    12541 aacatggtga gattttcaaa gaggtacaca aacgccgtct gaatgccgtg attgctattt
    12601 cttttctgat tgccgccgtg ctgattactg cgttatggcg cgataaggac agcgtcgcta
    12661 ttgaaattgt caaaatcggc ggggcggtag ctttgggata ttttgcagga atcaacagag
    12721 gtaaagcgca aaccttggaa aagcaacggc gcgaaaaaga cagtgattag attgtagtaa
    12781 aaaaggtcgt ctgaaacgtt ttggcgaaac tcgcaaagct cgttttcaga cggc
//
LOCUS       DAWFKN010000058        12008 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFKN010000058 DAWFKN010000000
VERSION     DAWFKN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.058"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            69..2183
                     /locus_tag="WGR46_001525"
     CDS             69..2183
                     /locus_tag="WGR46_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0856436.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2320..3579
                     /locus_tag="WGR46_001526"
     CDS             2320..3579
                     /locus_tag="WGR46_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ0856437.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3947..5353
                     /locus_tag="WGR46_001527"
     CDS             3947..5353
                     /locus_tag="WGR46_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0856438.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5571..6785
                     /gene="gltS"
                     /locus_tag="WGR46_001528"
     CDS             5571..6785
                     /gene="gltS"
                     /locus_tag="WGR46_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0856439.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6884..7900
                     /locus_tag="WGR46_001529"
     CDS             6884..7900
                     /locus_tag="WGR46_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0856440.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AASDAGVPQAAEDAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7911..8108
                     /locus_tag="WGR46_001530"
     CDS             7911..8108
                     /locus_tag="WGR46_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856441.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8126..8275
                     /locus_tag="WGR46_001531"
     CDS             8126..8275
                     /locus_tag="WGR46_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856442.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHKTAHKAL
                     PYVPS"
     gene            complement(8534..9937)
                     /locus_tag="WGR46_001532"
     CDS             complement(8534..9937)
                     /locus_tag="WGR46_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0856443.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAMAKQLWD
                     ANKLALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVF
                     EKEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRM
                     NSLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSAR
                     FKNHADIIGLQYTYKFK"
     gene            complement(10146..11618)
                     /gene="pyk"
                     /locus_tag="WGR46_001533"
     CDS             complement(10146..11618)
                     /gene="pyk"
                     /locus_tag="WGR46_001533"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0856444.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAIEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            11878..>12008
                     /locus_tag="WGR46_001534"
     CDS             11878..>12008
                     /locus_tag="WGR46_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856445.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGINKNTAVYYFHR
                     "
BASE COUNT         2820 a         3349 c         3027 g         2812 t
ORIGIN      
        1 aaagacttgg caggcaaagt attggccgaa gcagtcacgt tttaagacta ttcaggccgt
       61 ctgaaagaat gttcctcttt tcagacggcc tacaatccat taataacaat aatcgacgaa
      121 aacgcattgt gaaaaacgcc tacatcccct ctcgcggcat ccgcaaaatc ccctatctct
      181 ccactctatt gcctgagttt catatctgca aggatgggaa aggagcagag gctgttatcg
      241 gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccaccgaa caccagcttc
      301 cctttattgc tttggaagac ggctttttac gctcgctcgg actgggcgtc tccggttatc
      361 ccccctactc catcgtctat gacgacatcg gcatctacta cgacactacc cgcccttctc
      421 gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg gctcaggcgc
      481 aacagacgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac gcgcccgaac
      541 tttcagacga tcatccttta cgctccccat ccaagcctga aactgtcctc attatcgacc
      601 aaaccttcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg tttgaactga
      661 tgtttcagac ggccttaaat gaaaacccgc aagccgatct ttgggtcaaa acccaccccg
      721 atgttttgtg cggcaaaaaa caaggctatc tgacccagct ggcgcagcaa caccgcgtcc
      781 atcttttggc tgaagacatt aatccgattt cgctgctgca agcggtggac aaagtgtatt
      841 gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg gtaacgtttg
      901 gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa atcaaccgcc
      961 ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc gcagcctatc
     1021 tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc tttgatgtca
     1081 tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag ttatattgcg
     1141 tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta ccctcttgcc
     1201 gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc gacgatgcac
     1261 gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa caacaccaca
     1321 tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc tccaacttag
     1381 tgccgccgct gtcgctcgtt accgacgata tgagcattta tttcaatgcc gaaaccccgt
     1441 cccgtcttga atacatccta caaaaccaaa acttcgacga tcaagacttt cagacggcct
     1501 tgaagctgca aaaaatgctg accgaaaacc acatcagtaa atacaacgtc ggcagctcag
     1561 acttcaccgc cccgtcaacc gacaaaaccg tgatcctcgt tcccggccag gttgaagatg
     1621 atgcgtctat ccgctacggt tcgccccaaa tctaccgcaa tctggatttg ctccgtaccg
     1681 tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat gtagtcagcg
     1741 gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac caaaccgccg
     1801 agcaaaccga catcctgacc tgtctccaat acgcagacga agtacatacc atgacttcgc
     1861 tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc ctgccttttt
     1921 acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc cgcagacttg
     1981 agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc caccccgaaa
     2041 cccatcaggc cataaatgca gaaaccgcag cccaaatcct gatacgacaa aaaaatatgc
     2101 aaaaaaacaa caacggatta catcgcgggt actttgccaa aaaattaggt aaaatcaaac
     2161 aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat aaacttgcct
     2221 ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt gaaagttcaa
     2281 cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac cgtcctcaaa
     2341 aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca aggccctgtc
     2401 ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac cgtacataaa
     2461 ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca taccgttgtt
     2521 tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac tcaacatcac
     2581 atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc aaaacgcatt
     2641 gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg cccctactac
     2701 atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg tgccgacttt
     2761 tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac gccggtacac
     2821 ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt gttccgcaat
     2881 ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca ttacctcaaa
     2941 ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat ccaaatcgcc
     3001 aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca ggtattcaac
     3061 gacagccaag tccgtatcca ttgcgacttt cccagtgtcc gcagcttcct gctccatgtt
     3121 ttgagttcat ttgccgaaca cgcgcctgcc gataccaaca tcatcatcaa gcatcatccg
     3181 atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa agaacacccc
     3241 gaactcaaag gccgcgtgat ttatgttcat gatgtccccc tgcccgtttt cctgcgccac
     3301 ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggcctgat tcacaatatg
     3361 ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga ccaaaatacc
     3421 ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgtttca tgcctaccgt
     3481 atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt gtttttcccc
     3541 aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg aaagttcagg
     3601 ccgtctgaaa acatttcaga cggcctgaaa caatcaatac cttagctact gccatgtaaa
     3661 taaaacacaa aaacctgcat ttatcattaa caataaatta caaaaacagt ataatgaccg
     3721 agctgtcatg agcgcatacc gactcaacct gagccctttg taacacacaa aatatggatg
     3781 tatccctagg caaaacaata taacaagcca aacatcctaa agataagccg gcaaggcaac
     3841 acactctaca aaaccgtgcc aagcaatatt tttacaaagc cctcaaccgg tatcgccgcc
     3901 catatgccgc agcatccgtc ttccacttta tatccgcccg caaaccatga ccgccgctcc
     3961 tgatatcctc taccggcaag ccgccgccct tttggaacaa tccaataccg cccaagccct
     4021 gcccctgctg caacaggccg cagagcaggg atatacggaa gctgcttttg tattgggcaa
     4081 ccatctgctg aaaaacggtc atccggagca ggcacttcca tggttggaag ccgccgcggc
     4141 ccaacgccat cccaaggcac tcttcaccct gctgcaacaa cgcgaacaca acggcacccc
     4201 gaccggacag cttcttaacg aatacgcctg gctgagtgag caaggtcacc tcgaagccca
     4261 attaattctc atgcgttacc acacgcagcg caacgatcca caatcgctct actgggcgga
     4321 gcttgccgct gcccaatatg ccgcacctgc gtattaccat ttggcacgcc atcatcaacg
     4381 ccaaggcgac gttgcgacag ccatcgaaca atacgaaaaa gcggcagcac tcggcataac
     4441 tgctgcctgc tggcaactcg gtcaaatcta cttctacggt acaggtgtca gccccaacca
     4501 cgcacaagcc gaacactatc tcgaacaagc ggcgcaagcc ggccacatcg ccgcacaaac
     4561 cctgctggcc gaccttcttg ccgcccaacg caaacccgaa gccttagaat ggtatcgtcg
     4621 tgccgccaat aaggagcaag cggaagcaca agccaagctg gcccaatacg ccctgaccgg
     4681 cgaactttcc gaacgcgatc cgttccaagc ggcgcgctat gccaaagccg ctgccgagaa
     4741 agaccatcct gaagccctga aaatcatggg cgacctctac cgctacggcc tcggcatcaa
     4801 agctgacaac catatcgcgc acgattacta ccaccgtgct gccgcgctgg gttctgccgc
     4861 cgcagcacaa aaactcatca gcgacgccgc gctgtaccat ccgcaacaat acgaacaaat
     4921 caaaactgcc gccctgcaac aacaacaaac cgaaaccatc taccgtttgg cggaagcaca
     4981 agcctgcgcc atcggccgtc ccgccgacta caatgccgcg cgaaaaaatt acatggaagc
     5041 tgccgggttc caccataaaa acgcagcggc agccttaggc cgcatctacc attacggcct
     5101 cggtacggcg caagatcctc gggcggctgc acactggtac gccattgctg ccgaacaaaa
     5161 ccacccttcc gcccaatacc acctcgcctg tttttactat cacgggcaag gtgtcggctg
     5221 tcatgttccg accgcctgct actggctgca ggccgccatc ggcaacggcc acacttcggc
     5281 cgaatcatta atatccctat tagaacaatg gcgacgcgaa gcacaccatg ccatcggaca
     5341 aaaggccgtc tgaaaagatt tacactcgca ttttttgaca atctttaact attcccctaa
     5401 tatttgccag ttatttttca cggacacgcc attgttttca tttctttctg aaaacacctt
     5461 gtccgcgcat caataccatg acactcggcg gataacgcca agcgttgaaa cacactacat
     5521 ccggaacaaa aacggatgct cggaaaaata tttctaggag gtgaaacaac atggaatggg
     5581 aattcaacag ttattacaca ctgattgccg ccacgctcgt gttgctggtt ggtaaatttc
     5641 tggttcaaaa aatcaaattc ttacgagact tcaatattcc cgagccggta gccggcggtt
     5701 tgattgccgc tatcgtcctg ttcgccctgc acgaggcgta cggcgtgagc ttcaaatttg
     5761 agaaaccgct gcaaaatgcg tttatgctga ttttcttcac gtccatcggc ttgagcgcgg
     5821 atttttcccg tttgaaggcg ggcggtttgc cgctggtggt ttttaccgcg attgtgggcg
     5881 gatttatctt ggtgcaaaac tttgtcgggg tcggactggc tacggctttg ggtttggatc
     5941 cgctcatcgg tctgattacc ggttcggtgt cgctgacggg cggacacggt acgtcaggtg
     6001 cgtggggacc taattttgaa acgcaatacg gcttggtcgg cgcaaccggt ttgggtattg
     6061 catcggctac tttcgggctg gtgttcggcg gcctgatcgg cgggccggtt gcgcgccgcc
     6121 tgatcaacaa aatgggccgc aaaccggttg aaaacaaaaa acaggatcag gacgacaacg
     6181 cggacgacgt gttcgagcag gcaaaacgca cccgcctgat tacggcggaa tctgccgttg
     6241 aaacgcttgc catgtttgcc gcgtgtttgg cgtttgccga gattatggac ggcttcgaca
     6301 aagaatatct gttcgacctg cccaaattcg tgtggtgtct gtttggcggc gtggtcatcc
     6361 gcaacatcct cactgccgca ttcaaggtca atatgttcga ccgcgccatc gatgtgttcg
     6421 gcaatgcttc gctttcgctt ttcttggcaa tggcgttgct gaatttgaaa ctgtgggagc
     6481 tgaccggttt ggcggggcct gtaaccgtga ttcttgccgt acaaaccgtg gtgatggttt
     6541 tgtacgcgac ttttgttacc tatgtcttta tggggcgcga ctatgatgcg gcagtattgg
     6601 ctgccggcca ttgcggtttc ggcttgggtg caacgccgac ggcggtggca aatatgcagt
     6661 ccgtcacgca tactttcggc gcgtcgcata aggcgttttt gattgtgcct atggtcggcg
     6721 cgttcttcgt cgatttgatt aatgccgcga ttctcaccgg ttttgtgaat ttctttaaag
     6781 gctgattttc cgcctttccg acaaagcacc tgcaaggttt accgcctgca ggtgcttttg
     6841 ctatgatagc cgctatcggt ctgcaccgtt tggaaggaac atcatgtatc ggaaactcat
     6901 tgcgctgccg tttgccctgc tgcttgccgc ttgcggcagg gaagaaccgc ccaaggcatt
     6961 ggaatgcgcc aaccccgccg tgttgcaagg catacgcggc aatattcagg aaacgctcac
     7021 gcaggaagcg cgttctttcg cgcgcgaaga cggcaggcag tttgtcgatg ccgacaaaat
     7081 tatcgccgcc gcctacggtt tggcgttttc tttggaacac gcttcggaaa cgcaggaagg
     7141 cgggcgcacg ttctgtatcg ccgatttgaa cattaccgtg ccgtctgaaa cgcttgccga
     7201 tgccaaggca aacagccccc tgctgtacgg ggaaaccgct ttgtcggata ttgtgcggca
     7261 gaagacgggc ggcaatgtcg agtttaaaga cggcgtattg acggcagccg tccgcttcct
     7321 gcccgtcaaa gacggtcaga cggcatttgt cgacaacacg gtcggtatgg cggcgcaaac
     7381 gctgtctgtc gcgttgctgc cttacggcgt gaagagcatc gtgatgatag acggcaaggc
     7441 ggtaaaaaaa gaagacgcgg tcaggatttt gagcggaaaa gcccgtgaag aagaaccgtc
     7501 caaacccacg cccgaagaca ttttggaaca taatgccgcc gccagcgatg cgggcgtacc
     7561 ccaagccgca gaagacgcgc ccgaaccgga aatcctgcat cccgacgacg gcgagcgtgc
     7621 cgataccgtt accgtatcac ggggcgaagt ggaagaggcg cgcgtacaaa accagcgtgc
     7681 ggaatccgaa attaccaaac tttggggagg actcgatacc gacgtgcaaa aagagttggt
     7741 cggcgaacaa cgcaagtggg cgcaggaaaa aatcagcaac tgccgacaag ccgccgcgca
     7801 ggcagaccgg caggaatacg ccgaatacct caagctgcaa tgcgacacgc ggatgacgcg
     7861 cgaacggata cagtatcttc gcggctattc catcgattag gggcaaaccg atgaataccg
     7921 tcccaaaaag caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag
     7981 tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg
     8041 ccagattggg cgaaaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg
     8101 gcagataaag agaaaattga cggaaatgcc gtctgaaacc ctgttgtcgg tttcagacgg
     8161 cattttgacc aatacggtac gcaggcgcaa aacagccgtc ttttcctgcg tttcctatgc
     8221 tgatgtttca acacacaaga cggcacataa agcgttgccc tatgtgccgt cctgattcgg
     8281 aaggggttac gcccctccca aatatagtgg attaacaaaa accagtacgg cgttgcctcg
     8341 ccttagctca aagagaacga ttctttaggg tgctgaagca ccaagtgaat cggttccgta
     8401 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaat
     8461 cgagcctaaa acaatgccgt ctgaaacgga aatctgtttc agacggcatt gttacattca
     8521 aacggcgggc cgtttatttg aatttgtagg tgtattgcag accgatgatg tcggcgtggt
     8581 ttttgaaacg tgcggaagac gcgcctttgc tgtccacatc gttgccgctt gccttcgccg
     8641 tgcggtagct ggtgtcgttg atgtggatgt gggtgtaggc ggcatcgacg acgtggtttt
     8701 taccgatatg gtatttcata ccggcggaga accagatgcg gttgccgtcg ggtaggctgt
     8761 tcatgcggta gtcggcgttg cggacgggcg atttgtcaaa agcgatgccg gcgcgcagtt
     8821 gcagcggttc gctgatttga taagaaccgc cgaagccgac tttgtaggtg ttgcgccagt
     8881 tgggggtgat ggtggtgcgg tcggatttgc ctttgacgac ggttttttct ttttcaaaaa
     8941 ccagttccgc cttatcgaag cggctgtggc gcgtccaagt tacgtcgccg aacaggtcgg
     9001 ctttgtcgga cactttgtac atgccgtgta cggacaaaga ctcgggcgta acgattttaa
     9061 cgcgggcttt ttcgcttggc gtgtagccga gcagtgcgag tttgtttgca tcccataact
     9121 gtttcgccat tgcgccgtct gccgcccatt cggcatcgcc tttgagcgtg tgcgagactt
     9181 tggaacggta gttcacgccc acgcgcgcac ggtcgttgat gtcccacatc cacgccagtt
     9241 ggtagccgaa gccccaatcg ctgcctttga catcggcgtg tccgtcggcc tgaatttttg
     9301 cagcttcggc tacaccgtta ggtttgggcg gttttgccgt caatatctct gctttactct
     9361 taatccccca gtcggcatat ttgcgcagtt cggcggaagt atgttgggcg atgatgcctg
     9421 cgccgaagga atggcggtcg ttgagtttcc acgcggcgac aggttcgacg gcgatgctgg
     9481 tcagaccgag tttgttgatg ttgtggcgca acacggaatc tttttcgtat tcggtggcag
     9541 agccgaaggg gacgtacacg cccaagccca cggtcagatt gtcgttgact ttgtatgcgc
     9601 cgtagatgtg gggcgcgacc gtggttttgg tgattttgcc gcttttcgaa ccttggacgg
     9661 gaagcccggt aaagtcggtg gcggaatccg cctcataatg aatgctgggc agcacgatgt
     9721 tggcgttgac ggaaatctgg ctgctgtcga gtttggtcag gccggcaggg ttgtagaaga
     9781 tggtcgatgc gtcggcggct tctgcggcgg cggcatttgc cgtgctttgc gcgttgaccg
     9841 actgtgtgcc gaagtggtag ccggatgcgt ggacggatgc ggcggcaaag gcagtgccga
     9901 gcagcaggac ggtttttttc agtgcggaag gggtcatttc ggtttccgta aaaaggcgga
     9961 tggtggataa ataaaaaagg cagtcggaat tgccttgttt cgctttagta taggtactcg
    10021 attttatccg atgttgccgg atttgcacaa ttttttcagt ttgcccgaac cgccgcgccg
    10081 ccgcaaaaaa tgccgtctga agcctcgggc atcggcttca gacggcattt tccactcagg
    10141 gcggattatt tgacgcgcag cacttccagt gtgttggtcg aaccggattc gcgcatttgc
    10201 gagccgctgg tgatgatgta ttggtcgccg gaatgcagga tgttgtgttc caccagcatg
    10261 gtttcgactt cgttcaacgc cgtgtcgtgg tcggtgctgg ttgccaaaat cagcgggcgc
    10321 acgccccggt acatcgccat acggcgttgg gcggaaacgc tcggggtcag cgcgaaaatc
    10381 ggcagggtga tgttgtggcg gctgatttca aaggcggtcg aaccgctttc ggtcagggcg
    10441 acgatggctt tggcgtgaac cgcgcgcgcc acgctgaccg caccgccggc aaccgccagg
    10501 ttggtgctga ccgcttcggg atactcgacc tgttcggcaa cgccgttgag cgaatcctgc
    10561 tctttttccg cagccgcgca gataatcgcc atttggctga cggtttcaaa cggatacgcg
    10621 ccgacggcgg tttcggcgga acacatcacc gcatcggtgc cgtccaatac cgcgtttgcc
    10681 acatcgctga cttccgcgcg ggtcggcacg gggttggtaa tcatcgattc catcatttgc
    10741 gtcgccgtaa tgctgaagcg gcgcaactcg cgggcgcggc ggatcatccg tttttgcagg
    10801 gcggggacgg cggcgtgtcc gacttcgatc gccaagtcgc cgcgtgcaac cataatgccg
    10861 tcgccggcga ggatgatttc atcaaggttt tcaatcgctt ctacgcgttc gattttggaa
    10921 accaaaccgg ggcgcacggc cgtgctgcct ttcatttctt cttcgacttt ggcgcgcgcg
    10981 atgtgcaaat cttcggcgga tttcacaaag ctgatggcga gatagtcgca accgatggca
    11041 atcgcggttt tcaggtcgcg gaagtctttt tcggtcaacg cgcctgcgga cagaccgccg
    11101 ccgcgtttgt tgatgccctt gttgcttttc aggacgtggc tgttttccac ccttgtgata
    11161 atcctgctgc cttcgacgga ttccacggtc agggtcagca ggccgtcgtc cagccacaag
    11221 acatcgcctg cggcaacgtc gtcgggcagg tcgcggtagt ccaaaccgac cgcctcgcgc
    11281 gtgccttcgc cttcgagcgc ggcatccagt accagcgttt cgcctttgtt caattcgatg
    11341 ccgccgccgg cgattttgcc cacgcggatt ttcgggccct gcaggtcggc aatgatggcg
    11401 atttcctgtc cggtgcgttt tgccgcctcg cgcacgatgc gggcgttttc ctgatggaat
    11461 tcgggcgtgc cgtggctgaa gttgaagcgg acgacgttca ggccgccgac gcggatcata
    11521 tcttccaaca gttctacgtt gttgctgccc ggcccaaggg tggcgacgat tttggtgttg
    11581 tggctgatgc gggtcagatc gcggcttgtc tggttcatat gaaagtcctt tcggtctcaa
    11641 tcgggtgttt tgcggtattt tgttgcaaaa ttacagaaat ttggaaccgg tttgatgcca
    11701 tttgatgaac gcggcggaat attctgtaaa aatatgattt aaattaatag tttgatattt
    11761 tacctgcaaa ccgccttttt tggtgcaaaa ttacacggtt ttatgactta ggctaaattt
    11821 atttttggga ctgtcctaga taactaggga aattcaaatt aagttagaat tatccctatg
    11881 agaaaaagtc gtctaagccg gtataaacaa aataaactca ttgagctatt tgtcgcaggt
    11941 gtaactgcaa gaacagcagc agagttagta ggcattaata aaaataccgc agtctattat
    12001 tttcatcg
//
LOCUS       DAWFKN010000059        11973 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFKN010000059 DAWFKN010000000
VERSION     DAWFKN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.059"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            141..575
                     /locus_tag="WGR46_001535"
     CDS             141..575
                     /locus_tag="WGR46_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0856446.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WGR46_001536"
     CDS             complement(624..1967)
                     /locus_tag="WGR46_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0856447.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WGR46_001537"
     CDS             complement(1972..2625)
                     /locus_tag="WGR46_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0856448.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WGR46_001538"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WGR46_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0856449.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WGR46_001539"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WGR46_001539"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0856450.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WGR46_001540"
     CDS             4571..5503
                     /locus_tag="WGR46_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0856451.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WGR46_001541"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WGR46_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0856452.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7068..8078
                     /locus_tag="WGR46_001542"
     CDS             7068..8078
                     /locus_tag="WGR46_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0856453.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGR46_001543"
     CDS             complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGR46_001543"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0856454.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9061..10110
                     /locus_tag="WGR46_001544"
     CDS             9061..10110
                     /locus_tag="WGR46_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0856455.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPNDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(10179..11720)
                     /locus_tag="WGR46_001545"
     CDS             complement(10179..11720)
                     /locus_tag="WGR46_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0856456.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3073 a         3623 c         2972 g         2305 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacggc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cgttatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct tccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaactcaa atgcgaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgatgtgaac aaatactgcc gttgagcagc ctcatcgaaa accccatgcc
      601 gtctgaaacg gcacggggtt tgtttatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcacctag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcaccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttggc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg tttggcggcg
     1201 ttgcacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcgcgttcc
     1261 aaatcggcat cgggcatcac gataaaggcg ttgctgccgc cgagttccaa gacggttttc
     1321 ttgaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgtcga gccggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccaaaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagctgga
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatag aaatggcaat cagcaacacc aaaattccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccatcaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 agcctgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg accaggccgg tccgatggcg caaaccgccg
     3781 aagactgcgc gattctgttg aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgaacgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggcgtgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttcag acggcattgc
     3961 aaaatactat caacctcctc aaaacccaag gcgcggaatt ggtcgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agtaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcggc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agttaaacgc cgcatcatga
     4201 tcggcactta tgtactgtca cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcatcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctt atggcggaaa aactgttcgg
     4861 cacagacatt ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgaccttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg tgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt accgacatcg aaagcaaaaa acgctcgatg taccgcaagc gttatgaaat
     5401 gctggacaac atggttgcag agatgaagga cggtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgctccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccggcg tggtggaatg tgctttgccg ggcgtactgc cggtaatgaa
     5701 ccgagaagtc gttgaaaaag ccatcaaatt gggtttggct ttggatgcga aaatcaaccg
     5761 gaaaaacgtg ttcgaccgta aaaactactt ctatcccgac ttaccaaaag gctatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgcgccca tatggaagaa gacgcgggca agtccgtaca
     5941 tgaaggcttg aacggcgcga ccggtatcga tttgaaccgc gccggcacgc ctttattgga
     6001 agtcgtatcc gaacccgaaa tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt
     6061 gcacagcttg gtaacctggc tgaacatttg cgacggcaat atggcggaag gttcgttccg
     6121 cgtcgatgcc aacgtatccg ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcagaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaacgatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagac gcgcacgact accgctactt
     6361 ccctgacccc gatttgctgc ccgtcatcat ttcagacgac cggttgcaaa aagcaaaagc
     6421 agaaatgccc gagctgccaa aagaaatggc ggcgcgtttc gtggcggatt atggcgtgag
     6481 cgactacgac gcgcgcctgc tcactgccag ccgcgtgcag gctgcctatt ttgaagaagc
     6541 cgctaaagag agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cacgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cacgcctcgc
     6661 cgctctggta ggcaaaatcg ccgacggcac attaagcagc aaattggcga aaaaagcctt
     6721 tgaagccatg tgggctgaac ccgaagcctc cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgataccg gcgcgattga agcgatggta gacgaagtgc tggcaaacaa
     6841 tgcaaaagcc gtggaacagt tcaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 gcaagtgatg aaggccagca aaggcaaagc caatcccgcg caggttcaag agctgattaa
     6961 agccaaactt gcctgataag caagatgccg tctgaaaccc aaatccgacg ttcagacggc
     7021 attttcacag tctgaccaca ttaaaacagc ataaaaaaca atattccatg aaccacacca
     7081 ttacactgcc cgaccaaacc acctttgccg ccggcgacgg tgaaaccgtt ttagccgccg
     7141 ccgcccgtca aaacctcaat ctgccccatt cctgcaaaag cggtgtctgc ggacaatgca
     7201 aagccgaact ggtcagcggc gatattcaaa tgggcggaca ctcggaacag gctttatccg
     7261 aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa agcgatatca
     7321 gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcactctg cccgcacgca
     7381 tcgaaaatat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg cccaaagccc
     7441 cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc aacgtcagcc
     7501 gcagctactc catcgccaat ccgcccgacc aagaaggcat tttggaactg cacatccgca
     7561 ggcgtgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa gtcaaagaaa
     7621 aaggcattgt ccgcgttaaa ggcccgctcg gttcatttac cctgcaggaa gacagcggca
     7681 aacccgtcat cctgctggca accggcacag gctacgcccc catccgcagc atcctgctcg
     7741 accttatccg ccaagacagc ggccgcgccg tccatttcta ctggggcgcg cgtcatcaag
     7801 atgatttgta tgccctcgaa gaagcacaag ggttggcatg ccgtctgaaa aacgcctgct
     7861 tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc cacgtacaag
     7921 acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc tgcggctctc
     7981 cggctatgac cgaacaggca aaaaatctgt ttgtgcaaca gcataagctg ccggaaaact
     8041 tgtttttctc cgacgcattc acgccgtccg catcataatt ccccggtata aaaaagattc
     8101 aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgta aaaaaatgcc
     8161 gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca aggcgacagc
     8221 cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg cagcctgctc
     8281 ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc ccaatgcggc
     8341 ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc caccaatacc
     8401 gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg gctctgatac
     8461 gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac acgcccttcc
     8521 acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa tggatgagca
     8581 tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc ttcagaatta
     8641 acttccttca gcagcaccat acggctgttg ggtctgccgc gtccgtcgac cgccgccaca
     8701 ttgaccgccg tcggctcgtt aacctgtgcg cgtaccgcct cgtccaacca ccgctcgaac
     8761 tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct gtaatcttcc
     8821 cgaatattgt gcaaatccat ttaccgctcc tcttgttatt tgaaagattc tacccccgca
     8881 cgcaaaccga tttcaaccgt tgcacaaact ttgtccccaa agccgcagcg acgatttcat
     8941 ccgcaaaacc gccgcatcag gtacaatatc gaaccgtccg actgaggacg gcattttatc
     9001 aacccgtcct gccgcatacg ccgcagaaga accgctttat caagcgagtt aggaaaaatg
     9061 atgtccaaac agcccaccag caaacgccaa tggcgcgacg gcgcagcccc gtctgccaag
     9121 aaaaccgcca aaccgttcaa aagcaaagcc cgtcccaaag acgaaacgga caaaaccgca
     9181 tcccaacctt acggacaaaa agcttcagac ggcatcaaac ctcaaaacct ccccaaacag
     9241 cgcgccgcca aagccaaaaa actcgtcgtc cgcaatccca accaaaaaat tatggaacac
     9301 gcgcgcgatt taaaagaacg ccgcagcgac ctatcgcgca tggaacccga acgcctgcaa
     9361 aaagtgcttg ccgcgtccgg cgtcggctcg cgccgcgaaa tggaagaatg gatcaacaac
     9421 ggctgggtaa cggtcaacgg caaaaccgct caactgggcg acaaagtcac ccccaacgac
     9481 cacgttaccg tcaaaggcag catcatcaaa ctcaaatggg ccgaccgcct gccgcgcatc
     9541 atcctatatt acaaacaaga aggcgaaatc gtttcccgcg acgacccgca aggccgcgtc
     9601 agcatattcg accgcctgcc gcaggccgcc agcagccgct gggtcgccat cggacgcttg
     9661 gacatcaaca ccagcggact tctgattctc accacctccg gcgaactcgt ccaacgtttc
     9721 gcccacccca gcttcgaagt cgaacgcgaa tacgccgtgc gcgtactggg cgggctgacc
     9781 ggcgaacaaa tgcgcgtcct caccgaagaa ggcgtgatgc tcgaagacgg cttggcaaaa
     9841 gtcgaacgca tccgcgaaca aggcggcgaa ggcgcgaaca aatggtacaa cgtcgtgatt
     9901 aaagaaggcc gcaaccgcga agtgcgccgc atttttgaaa gccaaggact caccgtcagc
     9961 cgcctcgtgc gcgtcggctt cggccccatc ggactgccca accgcctcaa acgcgggcag
    10021 ttctacgaac tcaaccccgc cgaagtcgcc aacatcctca aatgggcgga aatgctgctg
    10081 ccgggcgaat accgccgcaa aaaagcctaa acccgccaaa acacaaaaat gccgtctgaa
    10141 acatctgctg tttcagacgg cattttgttc gggcgttttc aggagaaaag gtcgagtgct
    10201 ttgacaaaga tcatcaccac gccgtaggcg agcggtatga cggccaatgc ccaacgccac
    10261 cagaccgata tgccgccgcc ggaaactttt tcgcccacaa ggtaagcgtc ggatatggcg
    10321 gtttcgtcat cggggttgcc gctgtgtgcg gcggttttga tgtctttttc gtggtgtttt
    10381 tcgtgtacgg atttgacggc gaggttgcac aacaaaccga taatcagcag acccgccatg
    10441 atgtacatgg ttacgctgta tgcctgtgcc gccggtatgc cgctgtcgat ttggctttgg
    10501 cgtatgtaat tgaccagtac cgggccgatg acggcggcgg ttgaccaggc cagcaggatg
    10561 cgtccgtgaa tcgcgccgac ctgataggtg ccgaacaggt ctttcaggta ggcgggaatg
    10621 gcggcaaatc cgccgccgta catggaaata atcacgcaaa agccgatgat gaacagggct
    10681 ttgctgccgc cctcgccgat ggagggaacg gcgaaataca gcagcgaacc gagtacgaag
    10741 aagatggtgt aggtgttttt gcgtccgatt ttgtcggaaa cgctcgacca caaaaagcgt
    10801 ccgcccatgt taaacaggct caggaggctg acgaagcctg ccgccgcacc tgcgccgact
    10861 gccgcctgcc tgcctatgga ggtttcggaa aagagttcct gaatcatcac ggatgcctga
    10921 cccaatacgc cgatgccggc agttacgttc aggcacaata cccagaacaa cagccaaaac
    10981 tgcggcgttt tcatggcttg ggacacgttg acatgattgc tgctgaccag cttgttttgc
    11041 gttttcggcg cggtatagcc ttcaggtttc cagccgtcgg caggtacgcg gatggtaaac
    11101 gcgccgaaca tcatcagtgc gaggtaaagc agacccaata cggcgaaggt ttcggcaacc
    11161 ccaaccgaag cagcgtttga aaaggtgttc atcagtgata cggaaagcgg cgaggccagc
    11221 attgcgccgc caccgaaacc cataatcgcc aaaccggtcg ccatacccgg cttgtcggga
    11281 aaccatttca tcagtgtgga aaccggcccg atgtagccca aacccaagcc tacgccgccg
    11341 atgacgccgt tgcccaaata gagcaggaag aggttgtgcg tacgcacgcc gaatgcggat
    11401 acgaagaagc ccaggctgaa acagcaggcg gcggcaaata tggctttgcg cggccctacc
    11461 cgttccatcc acgtaccgaa cagggcggcc gacgcgccca gcatcgcgag tgcgatactg
    11521 aaaatccaac ctacggtcgt cagcttccaa tctccggccg ccgattcggt tatgccgata
    11581 agtttggtca gcggcgcgtt gaatacggaa taggcgtaaa tctgcccgat ggcaaggtgt
    11641 accgccaatg ctgcgggcgg tacgagccaa cggttgaaac ccggcttggc aatgcttgcc
    11701 tcacggtcta aaaacttcat aacatcctct ttctgtcagt tgaaaaataa aatttcattt
    11761 gctcaatgga aacttattga aaattataaa aaaatatcgg gtcgggtttt tatccgcccc
    11821 aagatgcgcc gtctgaaaca tttcgggtgt acggaaaggt ttctgttttt tccgacaaat
    11881 tcctgcggct tttcgcttcc ggattcccgc ttttgcggga atgacgaatt aaagattatc
    11941 ttaaggtcaa gggactggat tcccgctttt gcg
//
LOCUS       DAWFKN010000060        11961 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFKN010000060 DAWFKN010000000
VERSION     DAWFKN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.060"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGR46_001546"
     CDS             complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGR46_001546"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0856457.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1783..2796)
                     /locus_tag="WGR46_001547"
     CDS             complement(1783..2796)
                     /locus_tag="WGR46_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0856458.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGR46_001548"
     CDS             complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGR46_001548"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0856459.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3538..4212)
                     /locus_tag="WGR46_001549"
     CDS             complement(3538..4212)
                     /locus_tag="WGR46_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856460.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4366..4450
                     /locus_tag="WGR46_001550"
     tRNA            4366..4450
                     /locus_tag="WGR46_001550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4400..4402,aa:Leu,seq:tag)
     gene            4971..6005
                     /gene="purM"
                     /locus_tag="WGR46_001551"
     CDS             4971..6005
                     /gene="purM"
                     /locus_tag="WGR46_001551"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0856461.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(6479..7135)
                     /locus_tag="WGR46_001552"
     CDS             complement(6479..7135)
                     /locus_tag="WGR46_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0856462.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGRSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(7132..8904)
                     /locus_tag="WGR46_001553"
     CDS             complement(7132..8904)
                     /locus_tag="WGR46_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0856463.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            9110..9724
                     /locus_tag="WGR46_001554"
     CDS             9110..9724
                     /locus_tag="WGR46_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0856464.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            9887..10504
                     /locus_tag="WGR46_001555"
     CDS             9887..10504
                     /locus_tag="WGR46_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0856465.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            10512..11375
                     /locus_tag="WGR46_001556"
     CDS             10512..11375
                     /locus_tag="WGR46_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0856466.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(11388..11822)
                     /locus_tag="WGR46_001557"
     CDS             complement(11388..11822)
                     /locus_tag="WGR46_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0856467.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
BASE COUNT         2891 a         2861 c         3255 g         2954 t
ORIGIN      
        1 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
       61 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      121 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      181 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      241 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      301 ggacgcgcag ttttttgacg ttgaggccga cgaggatttg tgcgccgatg ccgtagcttt
      361 tgctgtccca tttgtaggct tggtttgcac ctttgggcag ggtgcggtcg agcagggatg
      421 cgccgtcttc ggcgcggtgt aagaggatga cgacgccgct ttcggcttgt tggatgcgct
      481 caagggcttt gggcagcgac catgaatgac gcgggttagc ttggatgaag tccatgacgc
      541 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggagggcgtg cctttgacga
      601 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
      661 acggggtttg tacaggcgca ttgcccatgt cttcaagcag gctttcggta cggctgcggt
      721 attcgatgag gtcggcaatc gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
      781 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggaggcag
      841 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
      901 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
      961 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1021 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1081 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1141 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1201 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1261 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1321 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1381 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1441 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1501 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1561 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     1621 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     1681 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     1741 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     1801 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     1861 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgtct tcgcgccgaa
     1921 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     1981 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2041 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2101 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
     2161 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
     2221 caattgcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2281 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2341 ctccaccagc gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2401 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2461 ttgttgtaaa tcaaagagaa tccccccccc cgcgtttttt gaacggttgg tatcggccca
     2521 ataatcgtcg ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt
     2581 gcgggcatcc gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc
     2641 tgcatattcg tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat
     2701 ttcataatcg gcaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc
     2761 cacattgtaa atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga
     2821 tgtttgcgcg tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta
     2881 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
     2941 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
     3001 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
     3061 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
     3121 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
     3181 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
     3241 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
     3301 tctgtctgta cggccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
     3361 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
     3421 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccat ttgcctcttc aaagccgtgc
     3481 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
     3541 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
     3601 agccaaggca agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc
     3661 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat
     3721 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg
     3781 tacatccgag gcaacattca tccagccgct ttgcgccgaa cggcaagcca gcgaaacctt
     3841 gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgcc gggacaggca
     3901 aaccagccga aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac
     3961 gcctttacag cgtgaaaaca ccgaatccaa agccatgaaa agccgtttca gaccttcctc
     4021 attcccgctg ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa
     4081 aaccgaacgc tcaatcgaag tcgtccccat atccattacc gtctgcgccg tcagatacgc
     4141 cgtccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     4201 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     4261 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     4321 accttgcaca aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga cgtggcgaaa
     4381 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     4441 gtccgcacca ataatgataa ataaattata tagttaatgc ttatcgggac aaattagtgc
     4501 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     4561 acacgcagaa cggtacataa agtgccgcca tttgttccgc accgaatcgg aaacgggtgc
     4621 ttccgcccgc aatcagattc actgccccta aatgccgggg tttcaaccgg aaagaagaaa
     4681 atattgattt tgatgatggc atcaaaaaac cccttccgcc cgtctgccaa aaggaatggt
     4741 taagaaaagc aatgcgtctt ttgatggaac gcgatatata aggaattgag gggaagggca
     4801 ggcgggtttc tgcccgttaa tcctgcggac ggtcccgcca aacccgacac gcgcgggcat
     4861 atggcggatt gtttacaata ttgtctaaaa atctgaacct gcaaacgtga aataaggtag
     4921 aatacgcccc gttattttac cgtccaatct ccatttacca aggaaaaacg atgagtactt
     4981 cattgagtta ccgcgatgca ggtgtcgata tcgacgcagg cgaccaactg gtcgaaaaca
     5041 tcaaaccgtt tgccaaacgc acgatgcgcc cggaagtatt gggggatttg ggcggcttcg
     5101 gcgcgttggt cgaaatcggc aagaaatata aaaatcccgt attggtcagc ggtacggacg
     5161 gcgtgggtac caagctcaaa cttgcctttg attgggataa acacgatacg gtgggcatcg
     5221 accttgttgc aatgagtgtc aacgatattt tggttcaagg agcagaacct ctgtttttct
     5281 tggattattt cgcctgcggc aaattggatg ttccgcgcgc gaccgatgtg atcaaaggca
     5341 ttgcacaagg ttgcgaagaa tccggctgcg ccctgattgg cggggaaact gccgaaatgc
     5401 cgggtatgta tcccgtcgga gaatacgatt tggcgggttt tgctgtcggc gtggtggaaa
     5461 aagagaatgt cattaccggc cgcagcatcg gcgcgggcga tgtggtattg ggtttggctt
     5521 ccaacggcgc acattcaaac ggctattccc ttatccgtaa aatcatcgaa cgcgacaatc
     5581 ccgatttgga tgccgagttt gataatggca aaaccttgcg tgaggctgtt attgcgccga
     5641 cccgtctgta tgtgaaaccg attcttgccg ctttggaaaa atttaccatt aaaggtatgg
     5701 cacacattac cggcggcggc attaccgaaa acgtgccgcg cgtgttgcct gaaaacacgg
     5761 ttgcacaaat cgatgctaaa tcgtgggaat tgcccaagct cttccaatgg cttcaaaagg
     5821 cgggcaatgt ggaaacccaa gaaatgtacc ggacctttaa ctgcggcatc ggcatggtcg
     5881 ttattgttgc tgccgaagat gccgatgcgg ttcagggtct cttgggtgaa caaggtgaaa
     5941 cagtttaccg tttaggtttg atccgtgagc gtcagggaga cgagcatcaa acccaggttg
     6001 cctgattgct tctatagtga aatgccgtct caaacatggg tttggatggc atttttatag
     6061 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
     6121 ggcgaggcaa cgccgtactg gtttttgtta atccactata tattgattga tatgagggtg
     6181 ggttacacat gcttaaacga tataagccat tgtatattta tgggattcgg aatggaacat
     6241 tttaccctgc attagtattt ccgcttttta ttatgccgtc tgaaaatatc atgatgatgc
     6301 gttttcagac ggcatagctg atattgcagt atcagatagg ggcaaacgga aaacactaaa
     6361 ccgaacaata ttactgctat gccaagcgta cttatggggg cataagctta catgcttggt
     6421 ctgttatccg aatgaccgta cggggattaa accccctaat atgccgtctg aacccgcctt
     6481 attccagcac gggttggggg acgttgtgcc ggatggcgta aacagcggcc tgcacccggc
     6541 tgctgaggtt gagtttgcgg agcaggtttt gaacgtggac tttgacggtg gattcggcaa
     6601 gatcgaggtg gcgggcgatg attttgttgc tgcgtcctgc ggcgagatag cccaagattt
     6661 ccagttcgcg aggggtaagt gaggagagtg cctgtgtccc ttgggcaggt tggggggaaa
     6721 tcaggctttt aacgagtttg gcggtcatct cgggcgagaa tacattatcg ccttcggcgg
     6781 ctttgcgtat gctttcgagc agaaagtcgg cgttgatgtt tttcagcagg tagccgcgcg
     6841 cgccgatgcg catacattcg gtcaagtcgt cgttgtcttc ggaaacggtc agcataatca
     6901 ctgcctgctg cggattgatg ctgatgattt gggagagtgc ttcgcgtccg ttcataccgg
     6961 gcatatcaag gtcaagcagg acgacatcgg gttggagccg gttgatcatt ttaatgcccg
     7021 agaggccgtc tgcggcttcg ccgatgacct caaaatcatg ttggcgcgac aaaagggatt
     7081 taatgccgct gcggaagagg gtatggtcgt ctatcaggat aattttaatg gtcatttcaa
     7141 gctttcttca gatacacccg ccaatgagac ggtggttccc tgttggggtt gggaatagat
     7201 tttcaatacg gcgcggatac gtttggcccg ctcctgcatg atgtgcagtc cgacatggct
     7261 gcccgtgggt tctcctattt tctccgtgtc gaaaccttgt ccgttgtctt ggatggtcat
     7321 ggtaaagcgt ccgccgtgtt cggaaagggt gaattttaca tgggtggcgc gggcgtgttt
     7381 gcggatgttg gacaggcttt cctgcaggat aaaaatcatt tggagctgcg cttcctgagg
     7441 cggcaggaac gaaccgtttt cccaggcggt ttcgaccgtt atcccggttt gttgcgtaaa
     7501 gcgggcgaat aggtcggcaa cggcttcggg aaattcttta ttgctgattt tggtacggaa
     7561 gttgagcagc agttcgcgga catcttcata acattcctgc acgcctgttt tgatgaagcc
     7621 gatgttttct gcggcttcct cccgtttgtt ttcggcaaag gcggtttcca gcatctgtac
     7681 ctgtaggttt aggaacgtta atgcttgtgc gatgctgtca tgtaatcctt gcgcaatcag
     7741 gttgcgttcc tgcaatactg caagcaggcg tttttcttcc tcctgttttg tgccggcaag
     7801 cgatacgccc aattgcctgc ctagtgtttg aagcaggatg cggtcgtctt catcaagaga
     7861 aatgccgttt ggaaagctga gcaacagcct gcccaatgtt tcgttctggt actcaatggg
     7921 gaagatttcc tcatggtact tccccaaatc cgaagctgct gtgccgcaat ccgcatgatg
     7981 aatggaaaca taaacatcgg attcgccgtc caaacaaact ctgccggaat ctgctcctac
     8041 ggcgggcagg atacggttta gaaaatgttc tgcagcctgt tgcggtatgt aggattggtg
     8101 caggtcccgt gtagtttggt acagcagggt caggttttga ttttgttttt cgagactgcg
     8161 tgtctgctcg gcgacttgtc cttccaaatc atcatataaa attttcaacc tgccgcccat
     8221 ttgattgaaa caacgcccga cctgtttgaa ttccggcgta ccgccttcgg gaaccggaat
     8281 atcgaaacac ctccgtccga tgcgttccgc accttccctt aacgcctgca gcggccggat
     8341 aacccaaatc tggtgccaaa acagcatcag tacagacgac accagcgtca tcaacataat
     8401 tgcccattga aaacgcctga gccaccatgt gtttttttcg ttggcatttt ccaatgcctg
     8461 caaaaacagt tcgatgtttc cggcaaagcg gtagagatcg acctgagtcg gtcgccggta
     8521 ggactggagc ggggggagga tgtgtgcctg ccaatctata atcagcatgg attgtatcaa
     8581 atcataagca agaggggtgt ccgaaggaat cagcggatgg atggcatcac tttgtgaaat
     8641 acgattcagg cttttttcaa attcgtcaac ctgattgtct atttgggagc ggggagatcc
     8701 ttcacccgcc atgtatgcca gacggtatgc ctgcattctc aagttgcccg cctcttcgat
     8761 gacggaggcc gcgttttcca gacgcaaaga gagcagcagt gtcaaaacga cagacaatgc
     8821 cgccaacccg acccacactc cggtcaggag tttcaggcgt agggaaaggc tgatgccgtc
     8881 tgaaaaacgg gctggcagta tcatgctcgg cgaaaaattg ttccaaataa tgcaaacaat
     8941 atcattcttt ggaattagat acaactgctc agaaagaatt ggttaaggaa aacttaatcc
     9001 gcaccgcttc agtgttaaat catgttatga taaaattcag ataatatgaa tttatttgcc
     9061 gtctgcttca gacggcattt tgagtcattg ggaacggcag actgcaatca tgaaaacctc
     9121 gaacttttcc aagagaacat ccgcaatgaa aacttttgcc ctgatactgg ccgacggtca
     9181 ggcgagccgt atgggaggcg aggacaaggg gcttgctctt ttggggggta aggcactgat
     9241 agatcatgtc atcgacaggg tcaggccgca ggtcagccat atcgccatca gcaccaaccg
     9301 gaatttggaa gaatatgctc gaagaagtcc gcatattttt ccggacgcac ggcagtggca
     9361 gcatttcggc ccgctttcgg cattgtgtac cgcagccaac gatttgcagt tggcgactgc
     9421 cgactggctt ttggttgtgc cgtgcgatat gccgtatctg ccggacgatt tggtggcgag
     9481 gtttgaaacc gtgtcgaaac gcacaccgtt gtgcaatgcg tattatgtgg aaacgccgat
     9541 aacgatgcac tacaacatta tgtatatccg cccgcagatt ctgcaaagcg cgattcccta
     9601 tctgttttcg ggtatgaaaa cattaagaag ctggttgcag cagcaaaggg cgcgaccggt
     9661 cagattcgag tttgacgggc attttgccga cttgaacacg caaactgatt tgcaggaggg
     9721 ataagggtaa gaccgccgac cggcgtggaa aggaaaggtc aagccgtacc gggcggtttt
     9781 tgcccgaatc ggaggcataa tgctgtctga aggcatttca gacggcattt ttcgcgggga
     9841 gatgctacaa tttgcaccat ttttaccgac acagggaaac aggattatgt ttacaggcat
     9901 tgttcaagga ttgggaaaac tgacggcaat ccaccgcccg tcggaggcat ttcagactta
     9961 tgtcgtcgag cttccgcaag aggcggcgga caatctgcaa cgcggcgcat cggtcgccaa
    10021 caacggctgc tgcctgacga ttaccgaaat cgaaggaaac cgcgtcagtt tcgatttaat
    10081 ggcggaaact ttggcaaaaa ccaatttagg gctgctgaag gaaggcgatt gcgtcaacat
    10141 cgaacgggcg gcgcgtttcg gagacgaaat cggcggacac gtcatgagcg gacacattat
    10201 ggcaaccgtg cctattgtcg aaatcgaacg ggacgggtac aaccgcacgg tttggttttc
    10261 gctcccgcac gaactcaaac cctatatcct gaccaaaggg ttcgtcggct tggacggttg
    10321 cagcctgacc ataggcaaag tcgaagacag ccgtttcaat gtccatttga ttcccgaaac
    10381 tttggaacgg acgctgttcg gcagcagaaa ggtcggcgac agaatcaaca tcgaaatcga
    10441 tccgaatacg caggcaatcg tcgataccgt cgaacggctg atggcgcaaa gatatgcaaa
    10501 gtgaggtaga gatggaattg aacgaatttc tcgataaagc ctatgccgtt ttgcggcgtt
    10561 tggatgccgt gcttccgccc gaacccgggc atacggattg gaacgcgctt gccttccgct
    10621 ggcagagtgc gggcaaaaaa ggttttttgg aacacttgcc cgatccgcac accttcccct
    10681 tggcaaggct ggcgggagtt ggcaggcaaa ccgaattgct ggtgcgtaat accgaacaat
    10741 tcattgccgg cagacccgcg aataacgtat tgatgagcgg cgcgcgcgga acaggcaaat
    10801 cctcgctggt caaagccctg ctgcacgaat atgcggataa gggattgcgc ctgatcgaag
    10861 tcgataaaag cgatttaatc ggcctgcctt acctgttgac gcttttgaaa gaacgtccgg
    10921 aaaaatttat cgtattttgc gacgatttgt cgtttgaaag cggcgatgaa acctataagg
    10981 cattgaaaac cgcgttagac ggcggtctgt ctcaacgttg cgccaacgtt ttggtttacg
    11041 cgacttccaa cagacggcac ctgatgcccg aatattttga cgagaatgcc ggtacgacgg
    11101 ggatgcgggg ggaaatccat cagaaagaag cggtggaaga aaaaatatcc ctgtccgacc
    11161 gattcggatt gtggctcagt ttttatccgt tcgatcaaaa cgattatctg gcggcggtgc
    11221 aaaattggct ggaagatttc ggcgtgccgt atgatgaaac cgcacagatg gcggcattgc
    11281 agtgggcgca gacgcggggc agccgttcgg gacgttccgc gtggcagttt gcctgcgact
    11341 gggcgggcag gctgccgaaa caacgggcgt tgtgagcgtt tcggggatta tttgaggcag
    11401 gaagaaagcc acatttgctg ctgttgcggt gtgaggatat ggaagaaccg gtgttggatt
    11461 tccaattcgt ccaccgcaaa atccataccg gacaaatagc ggctttcgac ataatcgcgc
    11521 gcctcgttcc gattaaaaac atccgaggaa atgatttcga cgacagaccg gcggcggctg
    11581 tgttcggaat gcataacctt caaacgcgcc ctgtcgcccg ccattttgaa ggcggcgcgg
    11641 attttacgca gttcattgtg ctgaccctgt gtcagtccga gccggcgtat gtcgcagttc
    11701 ggttgaaaat cgttcagggg gatataagcc gtggcggcgt gggaaagctg gcaggaaagc
    11761 aaagccatac ttaaaaaaga ggcggcaggt ttggcaaaac ggcagggagc aggcagtggc
    11821 acggcttttc ctcttgttgg aaattttagg aaatatacta gattgacgtg tgaaagatgg
    11881 tattatcgcg gttaactttt gttaataatg cgtacgtatg tccggcaggc agacataacg
    11941 gcggaatcgg aacgtatagt g
//
LOCUS       DAWFKN010000061        10949 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFKN010000061 DAWFKN010000000
VERSION     DAWFKN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.061"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..104)
                     /locus_tag="WGR46_001558"
     CDS             complement(<1..104)
                     /locus_tag="WGR46_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0856468.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPI"
     gene            291..473
                     /locus_tag="WGR46_001559"
     CDS             291..473
                     /locus_tag="WGR46_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856469.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(535..1536)
                     /locus_tag="WGR46_001560"
     CDS             complement(535..1536)
                     /locus_tag="WGR46_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0856470.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGGVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1613..5446)
                     /locus_tag="WGR46_001561"
     CDS             complement(1613..5446)
                     /locus_tag="WGR46_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0856471.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSNGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDETAVQTAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKERWDWLLANLNAPLADYKARYGAS
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLQIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5707..5803
                     /gene="ffs"
                     /locus_tag="WGR46_001562"
     ncRNA           5707..5803
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGR46_001562"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5970..7661
                     /gene="dld"
                     /locus_tag="WGR46_001563"
     CDS             5970..7661
                     /gene="dld"
                     /locus_tag="WGR46_001563"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0856472.1"
                     /translation="MSASQLLSRLTQTVGEKYIITAPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKIGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7793..7900
                     /locus_tag="WGR46_001564"
                     /pseudo
     CDS             7793..7900
                     /locus_tag="WGR46_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7956..8327
                     /locus_tag="WGR46_001565"
     CDS             7956..8327
                     /locus_tag="WGR46_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0856473.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8482..9030
                     /locus_tag="WGR46_001566"
     CDS             8482..9030
                     /locus_tag="WGR46_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0856474.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9165..10256
                     /locus_tag="WGR46_001567"
     CDS             9165..10256
                     /locus_tag="WGR46_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0856475.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10714..10884
                     /locus_tag="WGR46_001568"
     CDS             10714..10884
                     /locus_tag="WGR46_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0856476.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2527 a         2986 c         2980 g         2456 t
ORIGIN      
        1 tcgatcggct gccaatcaat cacctggtcc aacttcaata gcgggaagcg gttgatgtgt
       61 ttggcaatca tggcttgggc ggtttgccgg aagaaggtgc tcatgagaaa tctcctaaat
      121 gtcttggtgg gaatttaggg gattttgggg gattttgcaa aggtctctat ttgtactgtc
      181 ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc gtgcgtttgt
      241 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      301 atgtaatcgg cttttgctcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgtcggcg
      361 cattcggtat gcaggaagac gataccccgc aatcggatta tgaaaaacgt ttgggcttgg
      421 gtgcgaagct gaaaaacaaa aatacgccgg atacggaaga aaagcagcaa taaagatatg
      481 aaatgccgtc tgaaagattt tcagacggca tttgctttga agcatgggag acagtcaaac
      541 atttatctca tgctcaaatg cgttcaggat tcggcggact tcatccgcat ccttaaccgt
      601 tcggacgcgg tcaaacagcg tttgcgccgg gtcgaacgtt tttttcatca tgcccaacca
      661 ttgcttaaga cgggcgatcg ggtatttatt gtttgcctct tttgtcaggc acagctcgaa
      721 aaactgccgt atccatttag aaacttcggc aaaatccgtg tctttgaccg gcccgccgtt
      781 ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accccgccgc gaccgagcat
      841 cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc tgaaaacgtc
      901 gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc attcccaatg
      961 cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc cgcatgcgcc
     1021 cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt aaccgagccg
     1081 catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt tgacgatgtg
     1141 gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt tgacggtggg
     1201 cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg cttccagcgc
     1261 attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg gcgtgccgga
     1321 aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg atcgggaatg
     1381 cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc caatacgcgt
     1441 cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga tgattcgggt
     1501 tttttgtttg ggttcggttg tttgtccgtc aatcatgatt cgggtaggta tttgattcaa
     1561 acaatgccgt ctgaaggctt tcggcgttca gacggcattt ccgtgtccgt tgttacagca
     1621 atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc cagttttcgc
     1681 cgaggatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg ttgtcgtcgg
     1741 tgtagcggct caagccttgc aggcaggctg ggcaggaagt aagcattttg acgggttcac
     1801 cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaat ttgacttggg
     1861 tggcgatatc gggtcgtttg acggcgaaca taccggactc gccgcagcag cggtcgctta
     1921 agacgacttt ctgccccatc aggctgctgg ccatttgggt ggcgttcatg gttttgatgg
     1981 gggtgtggca ggggtcgtgg tagaggtatt gctgaccttg cacgccgtcg agtttcacgc
     2041 ctttttcgag caggtattcg tggatgtcga tgatgcggca gccggggaag atttcttcaa
     2101 agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg gtcttaatgt
     2161 cgaggtagtt gagggtgttt gccatacggt ggaaagccac gcggttgttg gtgctcattt
     2221 cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac atatagccgg
     2281 gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca agtccgattt
     2341 ggctgaacag gcgttccgaa ccgcaacccg ggaagtagaa cacggcttcg gcatcttcgg
     2401 gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc aataaggagc
     2461 gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg ataacctgtt
     2521 ctttaattgg ggctttgccg acggttgcct tgggttcggc tttttgcttt ttcgtgccga
     2581 tctgaagaag cttgccgatt ttgtaggcaa agttttgcgc tgggaagccg gtctgtatca
     2641 tggcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac gccataccca
     2701 tagctgcggc aggcgcaaat cgtttgtggc cggaatcggc aaggtagttg cggacggcta
     2761 cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg tggcacacgg
     2821 tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg ccgcggcggg
     2881 tttgttcttc gtataagaag gcttcggtca gcaagcccac gccgaggatt ttgttgcgcg
     2941 gactgtacag caggttggca cgcggaacgt gagtagagca gacgggtttg catttgccgc
     3001 agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtttgatttt tccataatca
     3061 gcgattccgc gcccaacagc tcgaaggacg gcgtgtaggc gttgcgtaag tccgagcctt
     3121 tcatcagttt gtggcggttg aaggtgtgtt tggggtcgac ttggtttttg tagtcccaaa
     3181 acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt gatgccgatg ccgtgttcgc
     3241 ccgaaatcac gccgccgagc gaacgggcga ttttcataat gcgttcgact gagcggtatg
     3301 ccgtctgaag catttcggca tcgtctgagt taaccggaat attggtgtga acgttaccgt
     3361 cgccggcgtg catatgcagg gcgacaaaga cgcggctgcg tacggttttg gcgtggattt
     3421 ttcccaagcc ttggataatt ttggtgtcgg ttttgccgct gaagatttca gaaagcggct
     3481 tcattacgtc cgctttgaca gacacgcgca ggcggaaatc gcggaatgcg gtaaagcagc
     3541 tttcattgtc tttggcttcg ggcgcggcgt ggacggatgc gccatagcgg gctttgtagt
     3601 cggcaagcgg cgcgttcaaa ttggcgagca accagtccca acgttctttg acggcggaaa
     3661 cgtgggcaag ggcgtgtttg ccgcgttctc ccaaaagttc ggcggtcggc aggtccgtac
     3721 ccattttgtc gatggggagt ttgcccgaca gatattgctc caaggcggcg cagagtttga
     3781 gtttgttttg gatagaaagc tcgatgttga tgcgttcgat gccgtctgaa tactcgccca
     3841 gccgttccag cggaatcacc acgtcttcgt tgattttaaa ggcgttggtg tgtttggcga
     3901 tggcggcggt gcggctgcgg tcgagccaga aggttttacg cgcttcgggc gacacggcga
     3961 taaagccttc gccgtcgcgt gcgcgggcga gttcgcagat gtgttcggcg gctgtctgta
     4021 cggcggtttc gtcgtcggaa accacgtccg ccagcaagac cattttcggc cgccccttgc
     4081 ctgccgcttt ggtggcgtag ccgacagcgc ggacataacg ccagtccaaa tgctccaaac
     4141 cggccagtcg tacgctttca tgggcaagca aaaagtcgcg gatttcgaca atagatggcg
     4201 tggcggtggc aaccgtgccg aaaaactcca tacacacggt gcgcgtgtgt ttcggcattt
     4261 tgtgcaatac gaaggcaacg ctggtgatga tgccgtccgt gccttctttc tgcacgccgg
     4321 gcagtccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg cggaacttgt
     4381 ggccggggat ttccaagcgt tcggttttaa cgatattgag gccgtttgaa tccagcgtgt
     4441 gaacgtcgaa cacggcggtt tcttcatcgt ggattttgcc gaaattgtgg cgcacgcgct
     4501 cgatgcgcag ccattcgcct tgagggttga ccatctgcca gtaggcgagg ttgtccaacg
     4561 ccgtccccca cagcacggct tttttgccgc ccgcgttcat cgccacattg ccgcccacgc
     4621 aggacgcgtc ggcggaagtc ggatcgacgg cgaacaccaa acctgcctga tgcgcggttt
     4681 cttccactcg tcgggttact acgcccgctc cgcaccggat aatcggatgc ttgccgtcca
     4741 agcccgccag ctcaacgtat tcaacaccac gatgcttgtc gagtttttcg gtattgatga
     4801 ccgcgctgtt cgcgtccaaa ggtaccgcgc cgccggtata acccgtgccg ccgccgcgcg
     4861 ggataataac caagtccaat tcgattaagg cgcgcaccaa aggcgcgatt tccgcctccg
     4921 tgtcgggatt gacgacgaca aaaggatatt cgacgcgcca gtcggtcgca tcggtaacgt
     4981 gcgttacgcg cgccaatccg tcaaacatga tgttgtgcgg cttggtaatt ttgcttaagc
     5041 gttccaaaat ttgatggcgt ttttggatgg tttcatcaaa actgctatca aaacgctcca
     5101 ctgctttttc tgctgcggca accaacacat cgacttgccg attatcgtcg cggcgtttgc
     5161 ggatttcgtt caacctgtgg cgcatttcgc gtaccagcgc ggcgcggcgt ttcgggtgct
     5221 ccagcaaatc atcgaccaga tacggattgc gcacgaccac ccaaatatcg cccaatactt
     5281 caaacagcat tcgtgccgaa cgcccggttt tacgctgtcc gcgcaggtct tgcaggattt
     5341 gccacgcttc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag gatgtatagt
     5401 tgtaggggat ttcccgaata cgctgcgggg cggtagtcgt ggtcatggtg tgtccgtgtt
     5461 tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgggttt gaaaaggcga
     5521 taatgtagtt caatttttga tatttttcaa tgccgaaaca ggaaataccc ttatagaaac
     5581 aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta ggtggtgttt
     5641 ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat aatgccgttt
     5701 ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc ggaagccagc
     5761 agcccactcc ggtgcgccag tgccgggggt ttggcccgcc gccctatttg aaacgccgga
     5821 gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg ttcagacggc
     5881 attttcttta ctttgccata acgtataact acaatgtgaa aacaattcca atgtgcaatg
     5941 cacctgccga caattttaag gaaactgcca tgagcgcatc acaattactg tcaagactga
     6001 cccaaaccgt aggcgaaaaa tacatcatca ccgcccccgc gaagaccgaa caataccgcc
     6061 aaggctaccg cttcggcgag ggcagggcgt tggcggtggt gcgccccgga accattctgg
     6121 aaatgtggaa aattctgcag gcctgcgtcg aagcggacgt gattgtgatt acgcaggcgg
     6181 cgaataccgg tttgacgggc ggctcgaccc ccgacggcaa cgattacgac cgcgacatcg
     6241 tgattgtgaa caccatgcgg atgaacatca tccaaaccat caacaacaac gaacaagtcg
     6301 tctgcctgcc cggctcgacc ctgaaccagc ttgaattgct gctgaaacct ttggggcgcg
     6361 aaccgcattc ggttatcggc tcttcctgca tcggtgcgtc cgtcttgggc ggcgtgtgca
     6421 acaactccgg cggcgcattg gtgcagcgcg gccctgccta caccgaaatg gcactgttcg
     6481 cccaaatcaa cgaagaaggc aggttggaac tggtcaacca cttgggcata gacttgggcg
     6541 acacgcccga agaaatcctg accaacctgc aaggtcatca ttatcaaaac aaagacatca
     6601 aacaagacgc gggcaaaggg cacgaccacg cctattgcga acacgtccgc caagtggacg
     6661 aaccgaccgc cgcgcgtttc aatgccgacc ccgcccgcca ttacgaagcc tcgggctgcg
     6721 cgggcaaact gatggttttt gccgtccgtt tggacacctt cccgcaagaa aaacaaaccg
     6781 ccgtgttcta catcggcacg aacgacatca acgagctgac cgacatccgc cgcgccgcct
     6841 tgggcgaatt tgaaagcctg cctgtttccg gcgaatacat ccaccgccac gctttcgaca
     6901 ttgccgacgt gtacggcaaa gacacgtttt acgtcatcaa aaaattcggt acgcaccaac
     6961 tgccgaaatt atttgacttg aaggcgcgcg tggacaggtt cggcaaaaaa gccagcttcc
     7021 tgcccaaaca tttttccgac aaggccatgc agttcgtcag caaatacctg cccgaccacc
     7081 tgcccaaatc catgcgcgat taccgcgaca aatacgaaca ccacctgatt ctgaaaatag
     7141 gcgggaaagg cgtggatgag gcgcgcgcgt tcttaaaaga atattttgca caccacagcg
     7201 gcgcgttttt cgagtgcaac gccgaagaaa cccaagccgc gatgctgcac cgtttcgccg
     7261 tcgcctccgc cgccatccgc taccgcgccg tgcatgatga cgaagtggaa gacttggtcg
     7321 cgctcgacat cgccctgcgc cgcgacgatc gcgactggtt tgaaaaactg ccgtcggaaa
     7381 tcgacaataa aatcatccat aaactctact atggacattt catgtgccac gttttccatc
     7441 aggattacat catcaaaaaa ggcaacgact gcatggcgtt ggaacacgaa atgctgcatc
     7501 ttttagacca acgcggcgcg caatatcccg ccgaacacaa cgtcggtcat ttgtatgaag
     7561 ccaaacccgc gctcaaacag ttttaccgca aacttgaccc gaccaacagc ttcaacccgg
     7621 gcgtaggcaa aaccagcaaa aagaaaaact gggcggaata agcgcgtccg ctttgaaggc
     7681 aggcaatgcc gtctgaaggc aaaacgccgt tcagacggca ttgtcgggtt tgaagttccc
     7741 cgtcgggtaa tcgctttcaa accgtatgcc gacacacggg cgcgcccctc ccaaatatag
     7801 tggattaaat ttaaatcagg acaaggcaac gaagccgcag acagtacaga tagtacggca
     7861 aggcgaggta acgccgtact ggtttaaatt taatccacta taaagtctga tcctaccttc
     7921 ccaaagggca gggtttcaac cgaaaaggaa acacgatgaa accatacaaa atctacaccc
     7981 atcccgccct gccgccccaa gccgtcaaac aaggctggtc atggccgggg ctcttgttcg
     8041 gcacgctgtg ggcgtgtttc aaaagaatgt ggggtttggg gctggggctg acgggcgcga
     8101 tattcgtgct ggctgtgttt gcccaactgg tttacggcga cacgcccgcc acagactcgg
     8161 cgtttaacgt attgggcttg gcggtttccg tctggttcgg cgcaaagggc aacagccttt
     8221 atgcacgcca ccttttatcg cgcggctata ccgaattgcc cgaaacggtt gaggcagcca
     8281 atccccaagc cgcattggcg caatatttcg ggcgcggggg ccgataagcg cgttttcttt
     8341 tccatcgtgc cgtctgaaaa atgttcagac ggcatttctt ataacctttc gtttgcagca
     8401 cataaccaat cagtctttcc tattccgttc cgaaatgcat accatgcgcc cgtacccaat
     8461 atttgcgaac aaggaaagaa aatggcacgt ttaaccgtac acaccctcga aaccgccccc
     8521 gaagccgcca aagcgcgcgt cgaggcggta cttcaaaaca acggctttat ccccaacctt
     8581 atcggcgtat tatcaaacgc ccccgaagcc ttggcgtttt accaagaagt cggcaagctc
     8641 aacgccgcca acagcctgac cgccggcgaa gtcgaagtaa tccagattat tgccgcccgc
     8701 accaaccaat gcggcttctg cgtggcaggg cacaccaaac tcgcaaccct gaaaaaactc
     8761 ctttccgaac aatccgtcaa agccgcgcgc gctttggcgg caggcgaatt tgacgatgct
     8821 aaactcggcg cgctcgccgc ctttacccaa gccgtaatgg caaaaaaagg cgcggtatcc
     8881 gacgaggaac tcaaagcatt ttttgatgcg ggctacaacc agcagcaggc agtcgaagtc
     8941 gtgatgggcg tagccttggc aactttgtgc aactacgtca acaacctcgg acaaaccgaa
     9001 atcaaccccg aattgcaggc ttacgcctga tacggcaaac cgcccgaata tcgggcggtt
     9061 tttcaaaaac accccctcaa acaaaaacaa gccgcctaag cggcagggaa gcagccgcgc
     9121 atcaggcgcg cccccgattt ccccgcaaac cttaaggaac aaagatgaac gcccaaaccc
     9181 tgattgccaa cgttgccgaa ttcgtcaaaa ccaagctcaa acccatagtg gacgacatcg
     9241 accgcaaagg atactacccc gaagcattta tgcgcgaact cggcgcaatc ggtggattcg
     9301 gcgcagtcgg tacagaagcc gaaggcggca acggcttggg tttggcaacg caaatcgccg
     9361 tattgcgcga aatcggcaaa gaatgcggcg caacctcttt cagtgcgtgg tgtcaggcgg
     9421 cttgcgcgtg gtatctgcac caaacgccca accgggccgt caaagacaaa taccttgccg
     9481 acatcctgca aggcaaagta ttggcgggta cgggcatgtc caataccgtc aaacaccttg
     9541 ccgacatcga aaaacacaac ctccaagccg agcgcgtgga gggcggctac acagtcaacg
     9601 gcgcgctgcc gtgggtgtcc aacatcggcg aagaccacat ttgggcgaat accgcccaaa
     9661 tcggcggcgg ctacgtcatg ttcatcacag gcggacaatg ggaaggcgta agcctgcaaa
     9721 actgccccga attttgcgcc ctcgaaggca cacgcacctt cagcctgaac ttcaaagacg
     9781 tatttatccc cgacgaagac atcatcgccg cgcccgaaca gtttgccgac tacatccaaa
     9841 gcatcaaagc aggctttatc ctcctgcaaa tcggcatcgg cgcgggcgtg atagacggca
     9901 gcctcggcat catccgcctc gccaacgtcg tcaacgccga agtcaaccgc tatctcgacg
     9961 acggctacga cagcctcaaa acaaggctgg acggcgcgtg ggcagaaacc gaacggctcg
    10021 ccggattggc ttggagcggc acgcccgaca accttaccac cctcaagctg cgcgaagccg
    10081 ccgccgtact gacacttgcc gccgcccaat ccgccgccct gcattccggc gcaaaaggct
    10141 acctgatgcg gagtcccgcc caaaggcgcg tccgcgaagc aatgtttgtc gccatcgtaa
    10201 ctcccgcgat caaacacctc cgcaaagaaa tagcggcaat cgaagccgca aaataaacag
    10261 gcggaaaaaa ccgaaacccc catcgcgtca ttcccgcgaa agcgggaatc cagcccccaa
    10321 cgtaacagga atccatcgga aaaaacagaa accctcaaac cgtcattccc gcaaaagcgg
    10381 gaatccagcc ccccctaacg caacaggaat tcatcgaaaa aaccgaaacc tccaaaccgt
    10441 cattcccacg gaagtgggaa tccagtaatc gaaaaaccac aggaatctat cgaaaaaaaa
    10501 ccaaccccaa aaaaaccggg cggataccgc accattctcc caaaccctga tttatagcgg
    10561 atgaacaaaa atcagggcaa ggcggcgaag ccgcagacag tacaaatagt acgggtgctt
    10621 cagtacttta gagaatcgtt ctcttcgagc taaggcgagg caacgccgta ccggtttttg
    10681 ttaacccact atacaaaaaa caaggaaaca caaatggcgc aatatatgtg cggcccctgc
    10741 ggctggattt acgatgaaga acacggcgac cccgaacacg gcatcgcccc cgggacaaag
    10801 tttgaagaca tccccgacga ctggaaatgc cccgaatgcg gcgtgggcaa agaagatttc
    10861 tacctgttgg atttcgtgat atagcggcag ccgcaccctt agggaaaacc ccgtcatccg
    10921 gcgttccgcc tgcggcggca gggaacacc
//
LOCUS       DAWFKN010000062        10727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFKN010000062 DAWFKN010000000
VERSION     DAWFKN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.062"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(293..505)
                     /locus_tag="WGR46_001569"
     CDS             complement(293..505)
                     /locus_tag="WGR46_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856477.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(517..798)
                     /locus_tag="WGR46_001570"
     CDS             complement(517..798)
                     /locus_tag="WGR46_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856478.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(836..1099)
                     /locus_tag="WGR46_001571"
     CDS             complement(836..1099)
                     /locus_tag="WGR46_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856479.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1127..1700)
                     /locus_tag="WGR46_001572"
                     /pseudo
     CDS             complement(1127..1700)
                     /locus_tag="WGR46_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1675..2019)
                     /locus_tag="WGR46_001573"
                     /pseudo
     CDS             complement(1675..2019)
                     /locus_tag="WGR46_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2012..2887)
                     /locus_tag="WGR46_001574"
     CDS             complement(2012..2887)
                     /locus_tag="WGR46_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0856480.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2932..3222)
                     /locus_tag="WGR46_001575"
     CDS             complement(2932..3222)
                     /locus_tag="WGR46_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0856481.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3234..3413)
                     /locus_tag="WGR46_001576"
     CDS             complement(3234..3413)
                     /locus_tag="WGR46_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0856482.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3527..3904
                     /locus_tag="WGR46_001577"
     CDS             3527..3904
                     /locus_tag="WGR46_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856483.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVSKGAEKKAAENTQVSATHKTA"
     gene            3933..4178
                     /locus_tag="WGR46_001578"
     CDS             3933..4178
                     /locus_tag="WGR46_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856484.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4412..5380
                     /locus_tag="WGR46_001579"
     CDS             4412..5380
                     /locus_tag="WGR46_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856485.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5377..5859
                     /locus_tag="WGR46_001580"
     CDS             5377..5859
                     /locus_tag="WGR46_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856486.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5949..6452
                     /locus_tag="WGR46_001581"
     CDS             5949..6452
                     /locus_tag="WGR46_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0856487.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6561..6893
                     /locus_tag="WGR46_001582"
     CDS             6561..6893
                     /locus_tag="WGR46_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856488.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            6890..7300
                     /locus_tag="WGR46_001583"
     CDS             6890..7300
                     /locus_tag="WGR46_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0856489.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            7315..8037
                     /locus_tag="WGR46_001584"
     CDS             7315..8037
                     /locus_tag="WGR46_001584"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0856490.1"
                     /translation="MNCCWGLSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPR
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGR46_001585"
     tmRNA           complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGR46_001585"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8783..9388)
                     /locus_tag="WGR46_001586"
     CDS             complement(8783..9388)
                     /locus_tag="WGR46_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0856491.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(9866..10051)
                     /locus_tag="WGR46_001587"
                     /pseudo
     CDS             complement(9866..10051)
                     /locus_tag="WGR46_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10139..10727)
                     /locus_tag="WGR46_001588"
                     /pseudo
     CDS             complement(10139..10727)
                     /locus_tag="WGR46_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216327.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2558 a         2494 c         2728 g         2947 t
ORIGIN      
        1 gcgcccgctg ccgcctaaaa ggcgaccgga tgcctgatta tcgggtatcc ggggaggatt
       61 aagggggtat ttgggtaaaa ttaggcggta tttggggcga aaatagacga aaacctgtgt
      121 tggggtttcg gctgtcggga gggaaaggaa ttttgcaaag gtctcagagt gagtttattt
      181 tggggcggcg gcgggtcggg ggcaagcggc gtggggcttg gttgtggttt ttaggtttgg
      241 gggtgggtaa aaaatgccgt acccggcggg caggcttcgc actctgaagg tttcagacgg
      301 cgtttgtttt ttctgtccaa tcgaggaact gccgccattt ttccagcgac atatcggtcc
      361 gccgcgtttc ggtatcgggt ttggcttccc agcggcctac ctgtatgtag tgcttcaccc
      421 cgacgatttg cgccaactcg gcctgtgtca gtttgcagcg gttgcgcagg gtgcgaaggt
      481 tgtaaggcgt gtagccgagt tccatgtcgt tgcggtttat tttgtttcgc atattttttt
      541 gactgcccgg cggcaggttt cggtaaggac ggcggcaaat cgggcttcgt ctgccggttt
      601 tccgtcgaaa aaaataaaat cgtaaagggt gatgccgttt tcggaatggc ggtaatgccc
      661 gcccgggcat tcgctgtctg tctgcctgtg gattttggca atcaggcggg gatagcggca
      721 atgggtaatg aaacggcttg cgccgtcttg cccgataatc cattcggggt agcggttgaa
      781 tagtgcggct ctgttcattt tgttcggggg ataaagcccc tcgcggggct tgtggtcagg
      841 caaatttgaa tatcagtgcc aacacggcgg cgatggcggt aaccagcccg gtggccgcca
      901 tcatgggata ccatcgggat tcttgggcta tttttacgga ttcggcgttg attttgtgcg
      961 cgtctgcaat gattttggcg atttcggcat ctacttttct cagactggag tgtttcattt
     1021 cttgttcgat tatgttcatc gtacttcctt tcgtttttgg cggttgccgc cgcttgtcgg
     1081 atggtaggat gtctgccatg tgtatatatt tatatattga taccttttag gttttattgc
     1141 aagtgttttg ggcggcggct tcgtatgctt ggcggtggcg gcggctgtac cattcggcga
     1201 gttcgcgccg ctcttggagg cggtagctgt cggcggtcag gtaatggtgc aggcttgaga
     1261 agccggcgcg ttggagggcg gcgcggctga tgtggccgag ggcgaggtct gttgtgaggg
     1321 tgtcttttcc ggcggtcagg ctgatgttgg tgaagaggtg gcggaatccg tgcattgtgt
     1381 gtttggattt gccgggggtg ctgccgtcat agcccagtcg tcggatggcg ttgtgggcga
     1441 atttgatgct gatgtggtcg ggatgggggg gcgggtttgc gccgtgggcg gatgcagggg
     1501 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1561 tccgacagtg ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1621 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1681 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1741 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1801 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1861 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1921 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1981 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     2041 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     2101 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2161 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2221 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2281 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2341 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2401 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2461 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2521 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2581 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2641 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2701 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2761 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2821 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2881 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2941 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     3001 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     3061 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3121 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3181 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3241 agtcccagct tcttggcaat ttcatgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3301 accaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtt
     3361 tggccgtttt gggcaaaatt ttcttttagt ttttcaacag tatagccata gatagccctt
     3421 tcccattgtg ataaaatagt gaactattta tgcactagaa attaattagt gcattaccaa
     3481 tgcgtgaatt atgagatttt aaaatctctt ttgcaaggat tattttgtga tttctaaatc
     3541 tctttttggt aatcgtttga aagaaaaaag aaagttttta gggcttaccc aagcacaagc
     3601 tgctgaaaaa gcaggtattg agcgcgaaac atggggcaaa tacgaacgtg gcgtatttat
     3661 gcctagtgga gatgtattgc tctctttttt gaatatggga atagatgtga gcagcttatt
     3721 tgctgctgaa cagggaatta ggccgtctga aaattctgaa attagtaagg aagagtcaga
     3781 gttattaggg tattaccgcc aagccagcga taacggcaaa tttgtcattc tcagtgtgtc
     3841 aaaaggcgca gagaaaaaag cagccgaaaa tactcaagtt tcagccacgc ataaaacagc
     3901 gtgattttta aatccaaaat ggagacagtc ttatgtttga ctttgaacaa caaatcaaat
     3961 ggggtgagcg agcggaagaa atcgttaaag aggcagccac ccagaacaat atcgaaattc
     4021 ccgaaccttt ggcttcggca ttagctaagg cggtaaaagt gcattatcta agccaagctg
     4081 gtgtgttttc tttggtagaa gcctatgcag acacggtaaa tccaactgaa aaagaagtag
     4141 attaccaagc aatcggcaaa gagctatttg aaaaataaaa aatgccgtct gaaattttca
     4201 gacggcattt aggacactcc gacagtcggg ctgctccggt gttctgaaac ggatattaag
     4261 caattgaagc ggccgtgcat tttaaaacgc attaaaaact accccgacat acaggtcatc
     4321 gggcaggtgg ttcaggtgtt gaaagatttg aattgatgcc gtctgaattt attttttcga
     4381 cacagatatt aaaagagaaa ggactgtaca aatggcaaaa aaaactacta aaactattca
     4441 ttatttacgt gcgcagcaaa accatagctt gttcgattta gaggtagcat taagaagggt
     4501 tttgtcagct gctcctactg ttcaagatac acaaattgaa caatataatc agcttaccca
     4561 aatcatgcat agaactttgg atcctaaaga tgtaaaaggc aattcaattg gtggattact
     4621 gatacacatt ggcagcggta caaaagatga acatatccgc actatgagca atagacctgt
     4681 acagcaagat gatcatggtg gaacacaagc tcctccaagt ggatattcct ttttgcgtaa
     4741 agaggctttt ttgtatattg ttggacacca tgtaattttt tgcggacatg gttttttgtc
     4801 agcatcaacg gttgcttctt atttaagttt acttagcaat aaattgagag agaacaatcc
     4861 ggatattgtt caatttaata ttgaattcaa agcagtaggc aattgcgaca agttgtcatt
     4921 gatacaacaa catggggtaa aaagtattct tttagatgct tcggcatatc aattatccag
     4981 aaacagactg tatcaagata gccgttcaac cattgctaaa gcattaggga aagttggtag
     5041 tgtatttaca agcgaattga gtgatgagga attggaagca caatccgaaa ttcatatcaa
     5101 tttagaagtc ttgctaaacg gtaattcgag agccagtatt gaagcgcaat cgctgatgca
     5161 ggagcaggca gaggaaatta ttgatgatga aacggttaat caaggatttt ccattaccac
     5221 gcaacaaggt gaagtgattc agccctctga tgtaaaactg tctaaatctg ttagaatagg
     5281 acgatacgat gaagccaact cgttactccc tgactcggca tttacagcaa tcagtgaata
     5341 tttcctagaa ctccaaagca gaaacttaac tgaacaatga aaaaatatct gcccttacta
     5401 cgttttttgt tgattgcttg tatatcagca tttttagcat ggaaagggca accttttgtt
     5461 catgggaacg agaaggccgt tgatttaatt attaacgtat ttgcaatctt agccggtttt
     5521 ttaattgcca ttatgacatt gtttagtgat atgaggtttg atgaagatgc aaattggcga
     5581 caaattcaaa ttcgtgaagg cgtacaagaa caacggtata taaaacattc cttacttttt
     5641 tacacatatc ttgccgtatt agtttgtgtt tttattgtca tcttgttggc tcataaagaa
     5701 gaatacaaaa atggaccagc cattttttgg cttgaacgga gctatttatt tttagcctgt
     5761 atttccattt tttattctgt attcttacct gggaatttaa ttaaaagccg aaaagaagag
     5821 tttaaaaaat taatggaaaa gaagaaaccc aaaatctaga acgagtttta acccccttta
     5881 aaatcccatt cagacgacct tttctaaaat ccctgtattg atttttaatt caatacaggg
     5941 atttttccat gtcagacgaa ttcaaccgat ttatcgaacg cgtcctctcc cacgagggcg
     6001 gttacgtcaa tcatcccaaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgca
     6061 ccgcacaggc aaacggctac aacggctcca tgcgcgccat gacgcgtgaa caggcaatcg
     6121 gcatttaccg taaagcgttt tgggagcgtt accgcgccga ccaaatgccc gaagcggtcg
     6181 cttttcaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctcc
     6241 aacgcgccgc aggcgtgccg gacgatggaa tcatcggcga aatcagcctc aaagccatca
     6301 attcccttcc cgaaaacgac cttttattgc ggttcaacgc cgagcgtctg gtcttttata
     6361 ccaagctggg tacgttcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
     6421 tgattcacgc cgccgcagac aatgccgatt gaggagcaaa ccatgtcaaa aaagtccatt
     6481 ctcgccctga tggccgcagc catgcagccc gatttcagcc acagcgacct aggcattcgc
     6541 tacgccatgc cgactcaggg atgttggacg caagcccacc gcaagagcgg ggtagccgcc
     6601 gcgaaacgcg cagccaaaaa aaagcgtcac aaataaccgc ctttttccga tggctgggcg
     6661 gcttggtctc taatccggcc acagggaaaa tcagccatac caaactttgg gcaaacgtcg
     6721 ccgcagccgc catgacttgg aagttcgtgc aggcggcgga cgcgcccgaa tggctttggt
     6781 gggcatacgg cgcgatggtc ggcgggtatg cattaatcaa acgcggcatc gccgtcgtac
     6841 cgcagttggc ggaagtcaaa aaatccgcaa atcaggaagg ggggcggcaa tgattgaatt
     6901 tgtccgagcc aaaaaacggc tgctttgggc atttgtgctt ttgcttgtgt ggacgtgcgg
     6961 ttaccgatac gccgccgaca aggccgaagc ggaacaaacc gccctgattg ccacctatcg
     7021 gcattcttct atggttgcgg cggaacaata tgccttgcag cttaaaaaag cgcaggacga
     7081 aaggcagcgg tggtacgact tttcccaaaa acaaggaaga aagcccgtga aaaaacagta
     7141 tccgccgcaa acgaaaaaag ccggctatct gaaaaccaag gaagaactgc ttgcggaatt
     7201 ggcttgcctt aaagcggaaa tggttgccct aaaaaagccc gatgccttaa tccatgggaa
     7261 agaagtgcgg cagaaagaac gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac
     7321 tgctgttggg gattgtcctt ctattaccaa ttggccgtcc aatcggcaga agacaaatat
     7381 gccgatttga aacggcatat ccatgatatt tatcgacgac ataagggaag atacggctac
     7441 cggaggattg cggcagccat ccgtcacgca ggaacaccgg tcaatcacaa gaaagtcagc
     7501 cgtctgatgg cgaagacggg gctgaaggca gtgatacggc ggcgcaaata ccgctcgttc
     7561 aaaggagaag tcggcaaaat tgcgccgaat atcctgcgac gctgtttcca tgcagaaaag
     7621 ccgaatgaga aatgggtaac ggacgttacc gagttcaatg taggcggaga aaagatatac
     7681 ctttctccga ttatggattt gtttaacggg gaaatcgtca gttaccgtat tcaaatccgc
     7741 ccgactttcg atttggccgg cgagatactg aaaggtgcgc cggagaaacc gagatcgtct
     7801 gaaaagccga tactgcattc ggatcaaggt tggcaatatc agatgttttt ttatagtgga
     7861 ttaaatttaa atcaggacaa ggcgatgaag ccgcagacag tacaaatagt acggcaaggc
     7921 gaggcaacgc cgtactggtt taaatttaat ccactataca acaacgatag aatcaagttg
     7981 aaattaaaag ggctgagcct tgttcagtac agaattcagt ccctgaaagc cgcttgatta
     8041 aactgtccga ctttttgggg tcagttcggc ttcggcattt ttttatccgt ttgggggtaa
     8101 cttgtttgga aagctgcaag cttataaata aaggattaca tttaagtttt gggtaacctt
     8161 tttaaaaaaa tgcgtgatga cttttgcatt cttaaggcgt tttttggggt aattcgtgaa
     8221 aagagttacc ccaaaagtta ccccataaat ggcgaaaact caagcatacg ccagcatatt
     8281 caaggcaaca aaaaagcctt gaaactgttg aagttcaagg cttttttgtg tttcaggata
     8341 ctgctgaaaa tagggtatgg tggaggcggg gggaatcgaa cccccgtccg aaagtcctct
     8401 acaaagcgtt ctacatactt agttgtgtct atttgaaaat cttatttcca tcatgccgac
     8461 caacaggcct tttggaaacc agttacctta aatctcattt cctgccaagt aacccggcag
     8521 gaaaccagtc aatgtaagat gacgttgcgg tggctttcgc cacacagccc attgaccgac
     8581 tgctgcaacg gctagcctta agcggctaaa gcgtaagttt cgtcgtttgc gactatttga
     8641 attcagtgtt ttacgggaat ctgagacccc ggtatgcccg catctgcttc gcaacccccg
     8701 tcgaaaccaa ggtcgccccc aaaaatggtt tgcaaattat acggatattg tgcggtgctg
     8761 ccaagtctgt cggagaaatt tgtcagtctt gctgccttaa tttgcgtttg agcaggatgc
     8821 ggacgcagcc gtcgttgcct tcccggggtt cggcgtaggc gagtacatcg gggtgttgca
     8881 tcagccagtt tcgggtcata tttttcagaa cgggtttgta gcctttggaa cctaatccgc
     8941 tgccgtggat gatttcgccg catacgccgc gtttttgggt gaatgcgatg aattcgttga
     9001 ggactttttg ggcttcttcc tgtgtgtagc cgtgcaggtc gacatcggta acgacgggat
     9061 agtatccgtt tttcaggcgt tggatgtcgt tttttccctg tccgtttttg ctgaagctgg
     9121 cgggcgggtc gttgtatgtg ctgccgatgt agaagtagtt ttcttcgtcg gcgcggttgt
     9181 ctttgggacg gactttgatg ggggttttgt cgggcggcgc ataatattgc tgccggtttt
     9241 ttaatgggga gacttgtccg actgcttgtg aaaaatcgaa atcctgttct tgtttttgct
     9301 tgtttttttc ggctgcctgt ttttctgccg cttctttttg ggcttgtttg cccagttgtt
     9361 tgaggatgtt ttggaagtcg gtattcatat tttttcctgt tatttgtccg atggctgttt
     9421 cgggcggggt tttaatttgc cggaatgttt gccaatcggg ggaggatgat tttgttgccg
     9481 tcgtatgttt tttgaaagtg tgattgtata tcaaaaagaa atgcggcaac cgtcggcagt
     9541 gttgattgcc ggaaatgcgg gccggtcgaa ccgatatgcc cgaacgcctg ataaagtttt
     9601 aaaaacctgc cttgcgaagc aggttaatgg ttttgccaat tttgaattgc cggaaacgcg
     9661 aaacacggaa atctgatgtt ttataaatgt tccgatacga actgcaaaat attggttgag
     9721 acctttgcaa aattcctttc cctcacgaca accgaaaccc caacacaggt tttcggctgt
     9781 tttcgcccca aatatctcct aattttaccc aaataccccc ttaatcctcc ccggataccc
     9841 tataatcagg catccgggct gccttttagg cggcagcggg cgcacttaac ctgttggcgg
     9901 ctttcaacag gttcaaacac atcgccttca gatggctttg tgcactcact ttaatcagtc
     9961 cgaaataggc tgcccgcgca tagcggaatt tacggtgcag cgtaccgaag ctctgttcga
    10021 ccacataacg ggtcttcgat aaataccggt tgagaccttt gcaaaattcc tttccctccc
    10081 gacagccgaa acccaaacac aggttttcgg ctgttttcgc ctcaaatacc tcctgatttt
    10141 acccaaatac ccccttaatc ctccctggat acccgataat caggcatccg gtcgcctttt
    10201 gggcggcagc gggcgcactt agcctgttgg cggctttcaa caggttcaaa cacatcgcca
    10261 cgggtcttcg acaaataccg gtcgcgtttg gtttgcgttt ccgtcagcgg acggttgcgg
    10321 caggctttgc gcataatgcc gtcctgcaac tgatgttctt ccagatgttg ccggttttcc
    10381 gcactgtcat agcctttgtc ggcatagacg gtcgtacctt tgggcagtcc ttccaacaaa
    10441 ggcggcaggt gtttgcactc atgggcattg gcgggggtga tgtgcagttt ctcgatatag
    10501 ccttccacat cggtacgggt atgttgtttg taaccgagtt tgtagaggcc gtttttcttg
    10561 atccaacggg catcgctgtc cttactcggt gtggtttggc cgctgatttg tccttcctca
    10621 tcgacttcta tagcctgacg ctgtttgctg ccggcggtct gaataatggt ggcgtcaacg
    10681 acggcggcgg atgctttctc tacttttagg cctttttcgg tcagttg
//
LOCUS       DAWFKN010000063        10718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFKN010000063 DAWFKN010000000
VERSION     DAWFKN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.063"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(63..1205)
                     /locus_tag="WGR46_001589"
     CDS             complement(63..1205)
                     /locus_tag="WGR46_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0856492.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WGR46_001590"
     CDS             complement(1254..1676)
                     /locus_tag="WGR46_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856493.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WGR46_001591"
     CDS             complement(1669..2478)
                     /locus_tag="WGR46_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0856494.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WGR46_001592"
     CDS             complement(2560..2937)
                     /locus_tag="WGR46_001592"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0856495.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WGR46_001593"
     CDS             complement(3137..3904)
                     /locus_tag="WGR46_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0856496.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..4693)
                     /locus_tag="WGR46_001594"
     CDS             complement(3914..4693)
                     /locus_tag="WGR46_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0856497.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPT
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVKIIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4692..4799)
                     /locus_tag="WGR46_001595"
                     /pseudo
     CDS             complement(4692..4799)
                     /locus_tag="WGR46_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4845..5573)
                     /gene="pdxJ"
                     /locus_tag="WGR46_001596"
     CDS             complement(4845..5573)
                     /gene="pdxJ"
                     /locus_tag="WGR46_001596"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0856498.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5597..6388)
                     /gene="recO"
                     /locus_tag="WGR46_001597"
     CDS             complement(5597..6388)
                     /gene="recO"
                     /locus_tag="WGR46_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0856499.1"
                     /translation="MSEHRINHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6422..7549)
                     /gene="pheA"
                     /locus_tag="WGR46_001598"
     CDS             complement(6422..7549)
                     /gene="pheA"
                     /locus_tag="WGR46_001598"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0856500.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACLTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7588..8820)
                     /locus_tag="WGR46_001599"
     CDS             complement(7588..8820)
                     /locus_tag="WGR46_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0856501.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9340..10194)
                     /locus_tag="WGR46_001600"
     CDS             complement(9340..10194)
                     /locus_tag="WGR46_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0856502.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2554 a         2876 c         2788 g         2500 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt acccaactgc
      241 ggataaagcg cctgcaaagc gtggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggtcgg acggactgcg cgtcggcaac gccgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcggg tttgcgcttc ggcgcggcgg
      481 cgcaaatccg cccacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaagcggcgc
      541 atcatgcccg gtgcgcggcc tgtggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggaggctttt gacgttggca
      901 cgctttgcct cgattttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gctttcgagt
     1141 tcttgcgata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcatagcgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcaa attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcaccccaaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccttttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttcc ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataatctt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgttggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc agtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatcattatt tattacatag tcatcgccgc gatatttaat atcccaaaat atagatttaa
     4501 aaccatcgtc tttgtggttt gtcagcattc ctaattgaga tagaaagtca aataatttct
     4561 tgtttctccg gctgtcatca agatggttta gattaagacc gcgaacaata caatacccaa
     4621 tacgctgtac tgtattgtga atttcttcta tcaaactatt attgtttgca gataaaatat
     4681 acttttgatt catatagtga attaaattta aaccagtacg gcgttggctt gccttgccgt
     4741 actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat ttaaatttaa tccactataa
     4801 catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct gcccttacgg cagcagcctt
     4861 gccctgaaca tcgcctcctt catctggcgc acggcttcgg gcagtccgag gaagagggct
     4921 tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg cgaggatttg ggcgatgggg
     4981 gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga ccaagcccaa atcgccggca
     5041 aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt gttcggcgtg gctgcgcgcg
     5101 tcggcatacg cgcctgtgtg cagctcgaca acgggcgcgc cgacatcata ggcggcttgg
     5161 atttgcctgt cgtcggcatc gataaagaga gagactcgga tgcctgcgtc ggtcaggatt
     5221 ttggtgaacc cggcgatttt ttcctgttgc gccaatacgt ccaagccgcc ttcggtcgtg
     5281 atttcctgac gtttttcagg cacgatgcac acgtcttccg gcatcacttt aagcgcgttt
     5341 tcgagcattt cttccgtcaa cgccatttca aggttcaggc gcgtgcggat ggcgtttttg
     5401 acggcaaaca catccgcgtc tttgatgtgg cggcggtctt cgcgcaggtg catggtaatc
     5461 aaatccgcac cgtgcgtttc ggcaaccagt gccgcctcca cggggctggg ataagtcgtg
     5521 ccgcgcgcat tgcggacggt agcgatgtgg tcgatgttga cacctaaaag cataatcttt
     5581 ccttttattt ctgccttcag acggcatttg aagccgtgcc gtccgaagtc gggacggttt
     5641 cccgggcggt ttctttgcgg tcaaactgcc gtatctgttc caacacctgc cgcgatttca
     5701 gcccctcggg caacaggtgg cggataaaaa gccgtgtgat tttcaatgcc tgttgcaggc
     5761 tttcggcagt gcggaaactg ccttcgcgca aatcgatcag gctctgccct tcgacaattg
     5821 cgccggcttc gtgcgaaagc gcgtctgcgt cctcgcatac gggcatgacc gcctcttcgg
     5881 gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat
     5941 cgggggcaac gcccaaggca ttgagcagct tccactcgaa gcggcgcaaa tcggcaatat
     6001 ggttggcttc acggcacacc gcctccatca cttttgccaa cgcgtcgtac agttcgggca
     6061 tcggatcttc gcgcgcggtc agtttcaaca ccagttcgtt cacatacaga ccgctgaaca
     6121 acgccctgcc ctgcggctgc cgccatccgc cgacccattc ggcacggtgc agggttttga
     6181 gttcctgact gccgtaccac gacgcactga cagggacgaa cggaaccaat acgccgcgca
     6241 gctcgctctg ccgtttgcgc gcgcttcttg ccaaaagtgc cacacgcccg taacggcggc
     6301 tgaaggcttc gacccgcagg ctgctttcgc gccagggcga tgatgccaac aggaagacgg
     6361 gttcgtggtt gatgcggtgt tcggacatca tgcctgaaat gccgtctgaa cgctgccgcc
     6421 gctacaaaac ggcggtcggg tacgaaccga tgactttgac gaacgaagcg cgttcgccca
     6481 agcgttccaa tgccgtctga atctgcgcgt cccggcggtg tccttcgatg tcgatgaaga
     6541 acaggtattc ccacaaaacg gatttgctcg gacggctctc aaacttggtc atggaaatac
     6601 ccgactcggt cagcggttgc agcagcgagg caaccgcacc ggcgcggttg ggcgcggaaa
     6661 cggccagcga agtcttgtcg ctgctgcttg caccggtttc gtgatgtccc atcaccaaga
     6721 agcgcgtggt gttgttcggt tcgtcttcga tgcactcggc aaccatatcg agtccgtaga
     6781 tttccgccgc cgtgcgtccg gcgatggcgg caaccgtacc gtcgtccgat tcggcaacca
     6841 gccttgcggc ttcggcattg ctggacacgg caatccgttc ggcgttgggc aggtgtctgc
     6901 ccaaccagtc gttgcactgc gccaacgcct gcgcgtggga aaagactttg gcaatgcctt
     6961 cggtgctgcc gttgttttta cgcaaaaggt tgtggtggat gcgcaaaacg acttcgccgc
     7021 acgcctgcaa cgcggtaacg gcaagcaaat ccaacgtgcg cccgaccgag ccttcggtcg
     7081 agttttccac gggggcgacc agatagtctg cctgacgggt ttcaacctgc ttgaagcagt
     7141 tgtctatggt cagacacgcc atggtgtgcg cggcgtgtcc gaaatgctta atcgccgcct
     7201 gctgggtaaa cgtgccctgc ggccccagat aggcgatggt cagcgggcgt tcgacggcga
     7261 ggcactcgct catcacttcc cgaaacaggc gtgctaccga ttcgtcgggc agcgggcctt
     7321 tgttcaaatc ctgaatgcgg cgcaacacgg cgacctcgcg ctccggacgg tatactgcgc
     7381 ccgtgccttt cagctcgccg atggcgtggg cgtgttgcgc gcgttcgttg agcaggcgca
     7441 ggatttcggc atcgatggtg tcgatggcgt tgcggtgggg aaggaggagt tcgtcgatag
     7501 tttgggacat aatttcgcct gaacagtaat ttatcgtgca ttctaacatc aaatgccatc
     7561 tgaaacggtt tcagacggca tttgatgtta aaggtattcg ctctgttcgt tttctttcca
     7621 cgcacgatgt gagcagagcc acagaagcgc aatgccgcaa gaggtggacg cggtcatggt
     7681 taccgccatc acggttgccg aaccgttgtg caagaaggtc gccgccatac ccacgcccgc
     7741 gccgattaag gactggaaca cgcccaacac ggcgttcgcg ctgccgccct cttctttgaa
     7801 ataagacata aagcatgcct gcgtgttcgc accgaccaag ccctgcgtgc cgacggaaaa
     7861 catcacgcac gcaaccagca gccaaaacgg gggcaacccg aaaaacagca cggcggcgag
     7921 ttgggacagg ttggcggcaa attggacgac aatcccccat agcaggatgc tttgcggatg
     7981 cgcgccggtt ttgagccgcc acgcggtaat gcggttgaaa aacatcatcg tgatgatgtt
     8041 gagtgcaaac gcccaagcgt attgatgagg cgtaacacgg taaagctgct ggtacacgaa
     8101 ggaagattcg gtcagaaagg cgaacatcga accgaagctg aatgcctgaa aaaacagata
     8161 acccatcgcg gcacgggttt tcaatacgcg tttgaaccgc ccagccacca gcccgaacac
     8221 atcccgtccg attttgcctc cgacggcagg cttgggcagg aaatactgca ccaaaccgag
     8281 cagcaccagc gaatacgccg ccagaaaaac aaaaatcgcc tgccagccac ccaagccctg
     8341 caacagtgcg ccgaccatgg gtgcgaccag cggcacaacc atcaaaatga tgccgataag
     8401 ggcaaacatc tgggcggctt tgcgtccgga ataataatcg cgcaccattg cgccgacgat
     8461 gaccacagtc atgcccgcac cgaatgcctg cacgacgcgc aggttgagga gctgttcggc
     8521 actcgaagca aatacgatgg cggcaacggc aaggcagtag acggccagtc cggtcagcgc
     8581 gacgggtttg cgccctttga tgtcggacac cgaaccgccg accacctgtc cgaacgccgt
     8641 gccgaacata aacaaactca ggctctgttc gatgcggtgg acatccgcgt tcagcgactg
     8701 cgccatttcg ggaatcgcgg gcaggtaggc atcgatggaa aacggcatca gcgtaaccag
     8761 catcgccatc aaaaccgcca tcagtttttc gctcatttca ggataatggg cagaaggcat
     8821 aaattctcgt ccgattcgca aacaaaccgc ccggcggcgg aaaacacaat acgcggaaac
     8881 ggaaaatttt aacacaccgg cacaaacagg cggcaacaaa agccccggca aaaccggacg
     8941 aaccggaacg atatagtgga ttaacaaaaa ccagtacagc gttgccttgc cttagctcaa
     9001 agagaacgat tctctaaggt gcttaagcac ccagtgaatc ggttccgtac tatctgtact
     9061 gtctgcggct tcgtcgtctt gtcctgattt ttgttaatcc gctataaaat cccgtcattc
     9121 ctgcgcaggc gggaatccgg acatccaacg ctgcagcaat ttgtcggaaa taaccgaaac
     9181 tcaaaaaacc ggatgcccgc ctgcgcggga atgacgatgc aaagatggtc gttatttcgg
     9241 ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga tggaaagatt
     9301 tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat gcgacagccg
     9361 gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca cgggcgacag
     9421 gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga gcgaaatcaa
     9481 cagtcccgaa acgttggcag gaacacggct catccccttg ttccacagcc aataggcgta
     9541 ccagccgcac cccaaaccca aatacagcag cgacaatacc atcccgacgc tccagtccac
     9601 ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg cggcggcaat
     9661 ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg tcggacgcat
     9721 agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc agccgacttc
     9781 gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg ccgccgcgcc
     9841 gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga caaacaccat
     9901 cagcagtggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt atttcaaccc
     9961 gacaaactga agcagcaggg tcagcacata gttgacgaac gacacaatca gcaacggctt
    10021 ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg caggcagcgc
    10081 ggcaattagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat agacatattt
    10141 ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa acatagtgag
    10201 tgataaagtc agatggtcat acaaaagtat agcggatgaa caaaaatcag gacaaggcga
    10261 cgaagccgca gacagtacag atagtacgaa accgattcac ttggtgcttc agcaccttag
    10321 agaatcgttc tctttgagct aaggcgaggt aacgccgtac tggtttttgt tcatccacta
    10381 taacagcaac cctgtcgccg tcattcccgc aaaagcggga atccagtccg ttcagtttcg
    10441 gtcatttccg ataaatgcct gctgcttttc atttctagat tcccactttc gtgggaatga
    10501 cggcggaagg gttttggttt tttccgataa attcttgagg cattgaaatt ccaaattccc
    10561 gcctgcgcgg gaatgacgat tcataagttt cccgaaattc caacataacc gaaacctgac
    10621 aataaccgta gcaactgaac cgtcattccc gcaaaagcgg gaatctagaa tctcagactt
    10681 tcagataatc tttgaatatt gctgttgttc taaggtct
//
LOCUS       DAWFKN010000064        10691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFKN010000064 DAWFKN010000000
VERSION     DAWFKN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.064"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(1..384)
                     /locus_tag="WGR46_001601"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="WGR46_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(494..781)
                     /locus_tag="WGR46_001602"
                     /pseudo
     CDS             complement(494..781)
                     /locus_tag="WGR46_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(889..1098)
                     /locus_tag="WGR46_001603"
                     /pseudo
     CDS             complement(889..1098)
                     /locus_tag="WGR46_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1095..2150)
                     /locus_tag="WGR46_001604"
     CDS             complement(1095..2150)
                     /locus_tag="WGR46_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856503.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(2256..2738)
                     /locus_tag="WGR46_001605"
     CDS             complement(2256..2738)
                     /locus_tag="WGR46_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0856504.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(2785..3921)
                     /locus_tag="WGR46_001606"
     CDS             complement(2785..3921)
                     /locus_tag="WGR46_001606"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0856505.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            complement(3918..4463)
                     /locus_tag="WGR46_001607"
     CDS             complement(3918..4463)
                     /locus_tag="WGR46_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0856506.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(4460..5935)
                     /locus_tag="WGR46_001608"
     CDS             complement(4460..5935)
                     /locus_tag="WGR46_001608"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0856507.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(6046..6411)
                     /locus_tag="WGR46_001609"
     CDS             complement(6046..6411)
                     /locus_tag="WGR46_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856508.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(6432..8342)
                     /locus_tag="WGR46_001610"
     CDS             complement(6432..8342)
                     /locus_tag="WGR46_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0856509.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(8361..9005)
                     /locus_tag="WGR46_001611"
     CDS             complement(8361..9005)
                     /locus_tag="WGR46_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0856510.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(9169..9987)
                     /gene="opc"
                     /locus_tag="WGR46_001612"
                     /gene_synonym="opcA"
     CDS             complement(9169..9987)
                     /gene="opc"
                     /locus_tag="WGR46_001612"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0856511.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            complement(10578..>10691)
                     /locus_tag="WGR46_001613"
     CDS             complement(10578..>10691)
                     /locus_tag="WGR46_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856512.1"
                     /translation="ARAAYFGLIKVSAQSHLKAMCLNLLKAANKLSAPAAA"
BASE COUNT         2312 a         2352 c         3033 g         2994 t
ORIGIN      
        1 catagacggt cgtacctttg ggcagtcctt ccagcaaagg cgacaggtgt ttgcactcat
       61 gggcattggc gggggtgatg tgcagtttct cgatatagcc ttctgcatcg gtacgtgtat
      121 gttgtttgta accgagtttg tagaggccgt ttttctttgt ccaacgggca tctttatcct
      181 tactcggtgt ggtttggccg ttgatttgtc cttcttcatc gacttctata gcctgacgct
      241 gtttgctgcc ggcggtctga ataatggtgg cgtcaatgct ttctctattt ttaaaccttt
      301 ttcggtcagt tggcggttaa tcagttccag caattcggac agggtgtcgt cttgcgccag
      361 ccagttgcgg tagcggcata aagtatagtg gattaacaaa aatcaggaca aggcgacgaa
      421 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt ttttgttaat
      481 ccactatact gctcagttcg tcgaaacggc aaaacaggtt gaaatcgatg cgggtaatga
      541 ggctgtgttc gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga
      601 acatggacaa cagcgggtag gcgggacggc cgcggtggtc tcgaaggtaa cgggtttttt
      661 gacggttcag gtactgctcg atcggctgcc aatcaatcac ctggtccaac ttcaatagcg
      721 ggaaacggtc gatgtgtttg gcaatcatgg cttgggcggt ttgttgaaag aaggtgctca
      781 tgagaaatcc cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg
      841 tctctggttt tgtttctgac aggcaaaagc actgtttatt tggctgtcaa aaggatggtt
      901 aaggaaagtt atgcgcccct gaagcgggcc ccagataagg atggttgcgc cgacggcttc
      961 agacggcatt ttggcggcgg tgttgggttt tgtatccggt ttgccgttgt gtcctgtgat
     1021 gatgattttg ggcgcggttt ttctgttttg atgtgtgaaa tgccgtctga aaggcggttc
     1081 agacggcata gcggtcattt ttgtgcggtc aggcggtcga atatgccgcc gtcggcgaag
     1141 taggttttca tgatgttgtc ccatccgccg aatttttctt cgggagagaa ggtgtctaag
     1201 tctgggaagt cggctttgtg tcttgccaat acttcggggt tgcgggggcg caggtagagt
     1261 gaggcggcga gttcttgcgc cggttcgctc caaaggtatt cgagataggc gcgggcggtt
     1321 ttttgcgtgc cttttttcgc gacgacgctg ttgacgacgg cgacggggct ttcggcggaa
     1381 atggtgtagc tcggatagac gatttcaaac tgtccttggg tcagtttttt gctgacgtag
     1441 ttggcttcgt tttcaaaagt gatgagtacg tcgccgatgt tgcgttgtgt gaaggtggtg
     1501 gtggcggcgc gtccgccgtt ttcaaaaacg ggggtgtttt tgaggatgga tgcgacgagt
     1561 ttttgggctt cctgttcgtt gccgttggtg gttttcagac cgtaaccgta tgcgccgagg
     1621 aaggcgtagc gtccgttgcc cgaggttttg ggattggcga tgacgatgtt aacgccgtct
     1681 ttggcaaggt cgttccaatc gcggatctgt ttggggttgt tttttcggac aaggaaaacc
     1741 atagtgctgg tgtagggcgc ggcgtggtcg gggagggctt gttgccagcc tttttctacc
     1801 agtccttttt tttcgagcag gtcgatgtcg gaggattggt tcatggttac gacatcggct
     1861 tgaaggccgt tggctacgga taatgcctgt ttgctggagc cgccgtggga ctgttggatg
     1921 ctgacggatg tgccggggtg ttcggattgg tatgttttga taaataaggg gttgtattct
     1981 ttgtaaaaat cccgtgccac atcgtatgag gcgttgagca gggtaatgtt ttttccgtcg
     2041 gattcggtat tggccggggc attttgtccg gacggatggt ttgaatcggc tgcggggctg
     2101 caggcggtga gcagggctgc ggtatagagt gccggtgcgt aggttttcat atgcttgtcc
     2161 tgtcggttgg tagatggggc aactttatac ggctgtctgc gcttgtggaa ataatgtttg
     2221 atttgaagat tatcagtttt ggttataagg acggatcaga ggtgtttccg catcagttcg
     2281 catttgattt tgatgctggg gtcaagctgc aatactgccg aaccgagcga ttcgtagcgg
     2341 ttgagaaggt aaaacgggac ggagttgagc gatgcgtaga gtttcagaaa gctcaaaccg
     2401 gatttgtggg cgatggtttc cgcctgatga agcagggcag tgcccagtcc gaggttgtgg
     2461 aacagggggt ggacgtaaag tgcatcgagt tgtgcttctt ggcagtcgat ttggaaaaat
     2521 ccctgtatgt tgcctttgta ttcggcaacc caaagtgctt tgtcgggatc ggaaatggtc
     2581 ggcaggtagc tttctgtgtt taacaagcct tcccatactt ttagggcgtg ttcgttgtag
     2641 ctgaggatgc aggtgtattg gacggagtgc aggtggactt tgaagatgtc tttgcagtct
     2701 tgcacggtgg cggggcgtaa cagtgtcagc aggttcatgg cagtatgtcg gcggcttcag
     2761 acggcatctg tgccgttggt cggattatag ggactgatgc agtttttttg cttcttgaaa
     2821 tgcggtgtcc gaatcggtgc ttaaaacggt aaagtgtccc attttccgcc ctttgtgcgc
     2881 ggtttttttg ccgtaaaggt gcaggtgcgc gtccggccgg ctttgcaggg gaaaccaatc
     2941 cggttcgccg ccgtcttcct gccaaacgtc gcccaaaata ttcgccatac agcaagaact
     3001 cagcaatttg gtgtcagcag gtggcaggtt gcacatcagg cggacctgtt gctggaattg
     3061 gtctgccgcg caggcgtcga cggtatggtg gccggaattg tgcggacgcg gcgcgatttc
     3121 gttgacgacc aattcatgcg tgtcgccgac aacaaacatt tctaccgcca atacgccgac
     3181 gtagttcaat tcatcggcca aacgctgcgc catttgtcgc gcctgttgct gaatgtcggc
     3241 actcagtcgg gctgggacga tggagtaggc gaggataccg ttttcgtgaa tgttttcggc
     3301 aggatcgaaa gtttgcacgt tgtcattgtt cagacggcat acgataacgg aaatttcgcc
     3361 gcgcaagtcc accatttttt ccaaaacgca atccacgccg cggtgttcgg caaacgcggc
     3421 tttgagttca tccaccgttt tgacgcggat ttggcctttg ccgtcatagc ccaatgtagc
     3481 ggttttcagg atgccgggca gaaattgtat gctttcttca gtgatgtctt cggctttgca
     3541 aattgcttga tacggcgcgg tttgcaggcc tgctttgcgt atccatgcct tttcctgaat
     3601 gcggttttgc gcgatggcaa cgcagtcgcc gctgggggaa acattggtat gtttggcgag
     3661 aaaacgcatc gcatcggcat tgacgttttc gaactcggtc gtaacagccg cacattttgc
     3721 caattcttcc aaagcggttt ggttgtcaaa cggcgcacac aaatggcggt cggcaaattc
     3781 cgctgccggc gcattcgggt tgggatcgag tacggttact ttgtagccca tggttttggc
     3841 agcaacagta aacattctgc ctaattgtcc gccgccaaga atgccgagca tggcgggcgg
     3901 agaaagagat atgtttttca tgctgactct tcaaattgta caagttgata gctataacta
     3961 attcttgacg gatgtcttgt atcgctggaa ttaccagttt cagaaataca gaatactttt
     4021 tccataaatt tttctgcttt tagaaattcc agtattctgt ttttttcatc cttataagca
     4081 ctgcggtctg taccccatgc aagaataatc atatcagcat cttccaaaca tcctttaaat
     4141 ttggaaaaat cggtttgggt gtttgcccta attcctgttt gctgtgtaga gtaactggaa
     4201 aaaatattta acattttgaa gttggtaaaa ccgtacatat ccaagaaacg tgcaagttgg
     4261 gtaagggttt tgtcgcttct ttcatcattt gctttacttg gattaattcc tatagcaaca
     4321 gctgagaaat ttttaggatt ttctgtgttg ccgctccatc taaccgttag gatttctcga
     4381 cttttttcat tatctgtata gagaccgtct ttggtagtcg gtctgagtgt ttggcgaagc
     4441 tcataaagtt tttcataagt catttatcca acccttcctg caccatctgc gcggcgcgta
     4501 taacggctcg ggctttgttt tgtgtttcct gccattcgga ggccggatcg gagtcggcaa
     4561 ccacgcccgc gccgctttgg acgtatagcg tgttgttttt tactacggcg gtgcggatgg
     4621 cgattgccaa atccatgtcg ttgttgaaac cccatacgcc gacggcaccg ccgtagatgc
     4681 cgcgtttgct cggttcgact tcttcgatga tttccatggc gcggactttg ggtgcgccgg
     4741 agagtgtgcc ggcagggaag gtagcggcga ggatgtccat gttggtcatg ccgtctttca
     4801 gacggccttc gacgttggaa acgatgtgca ttacatggga gtatttttca atcaccattt
     4861 tgtcggtaac tttgacttcg ccggttttac tgatgcggcc gacgtcgttg cgtcctaagt
     4921 caatcaacat gacgtgttcg gcgatttctt tggcatcgct taacaaatct tgttcgttgg
     4981 caaggtcttc ggcgggggtt ttgccgcgca ggcgcgtgcc ggcgatgggg cggacgatga
     5041 catcgtcgcg ttcgcggcgg acgaggattt cgggcgagga gccgacgatg tggaaatcgc
     5101 cgaaatcgta gtaaaagaga taaggcgaag ggttgagcgt acgcagggcg cggtagaggg
     5161 cgagcgggct gtcggtgaat tccatgctca tgcgctggct ggggacaacc tgcatgcagt
     5221 cgcctgcgaa gatgtagtct ttgattttgt taacgcaggc tttgaacggc tcttcgccga
     5281 attcgctgac ggcttcggtg tgtttgctgc cgagcgagag cgggatggcg cagctttggc
     5341 gcaactgggt gcggatgtct tcgaggcgtt cgcgggcgcg ttcgtagccg tcgggctgcg
     5401 acggatcggc gtaaacgacg agatggattt tgccgctcaa attgtcgatc accgccaatt
     5461 cttgcgacag catcagcaag atgtcgggcg tgccgagcgg gtcggctttg gtggtgtttt
     5521 tcaggcggtg ggcgaagtgt tcaaaattgt agatggtttc gtaaccgaag tagccgacca
     5581 gtccgccggt aaagcgcggc aggcttggga tttcgggtgt tttgaagcgg ttgtggaagg
     5641 cttcgataaa gggcaggggg ttgccgtcgt gttgctcgac aatttcgccg ttttgataaa
     5701 catcgacgtg tttgccgctg gctttgagat agtggctgca aggcaggccg ataaaagaat
     5761 agcggccgaa acgttcgcca ccgacaacgg attcgagcag gtaggtatag gggcggttgg
     5821 cgagtttgag atagagggaa agcggcgtat ccaaatcggc aaggagttct tgcacgagcg
     5881 ggatgcggtt gtagccttgg gcggcttggg cttggtattc ttgtttgctg atcatttctg
     5941 ctttcccaaa gggcggtttc ggacggcgcg gcaacgggcg cgagtatagc attttatcgg
     6001 aattgttgac agtctgaccg gagatgccgt ctgaaaagga cgggcttacg gctttgtttt
     6061 gcgctgccgg tattgtttcc atttaaacag ataacctgcg gttacgggaa tcagggctaa
     6121 gatggttttg aacgtccatg atgcatacca aggtaaagaa ccgggtgcga ccgattgatt
     6181 cagatacggc caccaacagg cagtcaggca gccgagcagc agcggccaga tatgccggct
     6241 gaggtttttt tgccaaagga ggaatgatgc ggcagaccaa agcagggtaa gggcagtgat
     6301 ttttaacacg gtataagcat ccgctccaaa cagggcggcg agaaaatacg taccggcaat
     6361 ccagaggacg gcaagtatca ggacaatgat ttcttcaggt cgtttgaaca tttttttctt
     6421 cctgtttgat ttcagacggc attgccgttc tgtttggttt ccagcagctc ccagcgttcc
     6481 agcttttcca aaagcagcat ttcgatttct tcggcgcggc tttgcagtgc gccggctttt
     6541 tcgtaatctt tgaagatttc gggatcggaa agctgcgcgt tgatttctgc ctgttcggct
     6601 tccaaggcgg cgatttcgtc gggcagggcg tcgagttcgc gctgttcttt gtaggacagt
     6661 ttgaccgtgc ggttggcttt gggtttttct ttcaccggtt cggcatcggc tgttttgggt
     6721 gcggattccg tctgaatctt cgcttcgcgt gattttgcgt cgatgtagtc ctgatagccg
     6781 ccgatatatt cttttaacct tccatttcct tcaaaaataa tgctttgggt aatcacgtta
     6841 tcgaggaaca tacggtcgtg tgagacaagg aataccgtgc cttgataatc gcgcagcaaa
     6901 tcttcaagca gctcttgggt atcgatatcc aagtcgttgg tcggttcgtc caaaatcaaa
     6961 atattagcag gacgggtaaa gagttttgcc agcaaaaggc ggttgcgttc gccgccggag
     7021 agcgatgaaa cgggactttg tgcgcgggct ggatggaata agaaatcttc cagatagctc
     7081 attacgtgtt ttttcttgcc gccgacttcg acgtaatcgt tgccttgacc gagcgtgtaa
     7141 aacacggtgt cgttttcgtt caacgtgctg cggaattggt cgaaataggc aacttcctgc
     7201 ttactgccga tacggattct gccgtaggtc ggctgcaatt cgcccaaaat cagcttgagg
     7261 aaggtggttt tgccgatacc gtttgggccg attaagccga ttttgtcgcc acgctgcaag
     7321 atggcggaga atttgtccat gatgactttg tcgccatagg taaacgaggc atgttccagt
     7381 tcggcaatga ttttgccgct tttctcgccg ctgtcgagct tgaaattgac ctgcccttgt
     7441 acgttgcggc gttcggcacg ctggcggcgc agttcttcca aacggcgcac gcggccttcg
     7501 ttgcgggtac ggcgcgcttc gatgcctttg cgtatccacg cttcttcctg cgcgtggaat
     7561 ttgtcgaaga ggcggttgtg ttccgcttcg actgccaact cttgcgcttt tttctcgcta
     7621 tatttggaga acgagccggg ataggaacgc aaaataccgc gatcgagttc gacaatccgc
     7681 gtggcgatat tgtccaaaaa acggcggtcg tgggtaatca caaccaggct gccttcaaac
     7741 gctttgagca ggttttccaa ccaaataatc gcgtcgatgt ccaaatggtt ggtcggttca
     7801 tcgagcagca atacgtcggg cttctgcacc caagcctgcg ccaaggcgac gcgctttttc
     7861 tgaccgccgg agaggttgcc gattttttcg ttttccggca aaccgagttc gcccaaagtc
     7921 tgcttcaccg ccgcatccaa cttccagccg tccttcgctt cgatctcaag ttgcaattcg
     7981 ttgagctctt tcaataaggc ctcactcgaa ccattttcca actcatggct gacatgatga
     8041 taacggcgca ataaatcgcg aatttcgccc aaaccttcgg caacagtatc aaatacggtt
     8101 gcttccttat caaaaaagga ttcctgcggt acataaacga ttttgaggtt gttttggaca
     8161 ataatctgcc catcatcaag cttttgcacg ccggcgagga tttttaaaaa cgaagactta
     8221 cccgcaccat tacgaccgat taagccgatt ttttcgcctc tgtcgagttg gaaggaagtt
     8281 ttgtcgagca gggcgacgtg gccgacggca aaggaagcgt tttctacaga taatatattc
     8341 atgatacaaa ttctcaacag ttaccgtttg gattttaccg caagtttggc gcgggcaatt
     8401 tcaaccgcac ccggcaggac ggaaacaata atgatgccgc ccatcaccaa gcccagattg
     8461 ttttttacga cggggaagtt ggcaaagaaa tagcccgcgt aagaaaacag gataacccac
     8521 aacaagccac cgatgatgtt gtagcggata aatttggcat agtgcatttt ccccataccg
     8581 gcgacgaagg gggcgaaggt gcggacgatg ggcataaaac gggcaatgat gatggttttg
     8641 ccgccgtgtt tttcgtaaaa acggtgggtt ttatcgagat attcacgtcg gaagatttta
     8701 gaatcgggat tggcgaacag cctgccgccg aaatatttgc cgacggtaaa attgagcgcg
     8761 tcgccgagta tggcggcaag gcttaataat gcaaccatca aatgaatatc cataccgccc
     8821 agcgcggcaa taccgccggc ggcaaacagc agcgaatcgc cgggcagtaa gggcgtaaca
     8881 atcaggccgg tttcgcaaaa aacaatcaaa aacagaatcg cataaatcca cacaccgtat
     8941 tgcgccgaca gcgcgagcag gtgttggtcg atatggagga tgaagtcgat ggcggaggca
     9001 agcacggcgc gttcctaaaa aaaacaaacc gcgtatttta accgattgga aaaatgccgt
     9061 ctgaaatttt cagacggcat cggctattca aattcatttc acgtaaaaac cgcaaaccaa
     9121 aatagtttgc ggtttggcat ttaaagtgac aatgatgatt tcaaatcatc agaattttat
     9181 gccgacgcgc aagccgtatt cacgaatact ggttttcggg atggtgagcg atacgtcgcc
     9241 actcttggtt gttacactaa actcgccgga ttctttgtaa gtgcgttgtt tgtagaacgg
     9301 ccccgcctcg atgctggcgg attcgcccag ttttttacca atatttgcac ccaatccaaa
     9361 gccccagccg ttggtttttt gattgatgtc ttttttgaga ttggtaacgc cggtgtttaa
     9421 tttatagcgg gaattgaggt caaatttcac ttcagaccaa gggttgatat accagccgtt
     9481 acccagttgg gaaagcaaat ccgcgtgaac tttggctaac cacgactgac ggctgctgtg
     9541 aagcgtatgc ttggtggttt taatgctgtc ttttgaagat tcaaaaccca agccggcacc
     9601 cacacggaaa tttaaagaat cacttaacgt ttgggtgtag gtgtagcctg tgtaaagatc
     9661 gatacggttt tcaggaacgc cggtgggcag ttttacattt ttagtcttac ccagcttgtt
     9721 ctcatctgtt tccaaattaa taatattttt tttgctgcgc ccgaaaccgg cttccaagcg
     9781 gatgccttgg ttggcatcaa aaggaatatc agcacgtacg ccgatgtgtt tggcagcttt
     9841 gtgtttttct ttcaggaaag cacgagttga agaaatggaa gagaggtcgg tgtggacggt
     9901 aaactcatta gcggtttgaa gctcttgtgc agcggcggca gtaccggtca gggcaatcat
     9961 ggcacatgta aaaactgttt ttttcatagt taaaatctcc aaaatttgga ttgtagtcgg
    10021 atatggtaac ataacgtaaa taatcgttac gcttacaatt atattcttaa gctttcgggg
    10081 ggggggggga ttttacgtat attaataaaa attaacaaat agttatttgt ttacaacgaa
    10141 ttgttattct cacttggttt tctgtttttt atgggaatga cgaaatttta ggtttctgat
    10201 ttcggttttc tgttttaagg gaatgacgag acttgagatg gcggcattta tcgggagcaa
    10261 ctgaaaccac tctgccgtca ttcccgcgaa agcgggaatc taggttcgtc cggtttcggt
    10321 tatttccgat agattcctgc cgcgttgggg gtctggattc ccgcctgcgc gggaatgacg
    10381 aatttcaggt tgctgttttt ggttttctgt ttttgtgaaa ataacaggat gagatctttg
    10441 caaaattcct ttccctcccg acagccgaaa cccaaacaca ggttttcgtc tattttcgcc
    10501 ccaaatacct cctaatttta cccaaatacc cccttaaccc tccccggata cccgataatc
    10561 aggcatccgg tctcctttta ggcggcagcg ggcgcactta gcttgttggc ggctttcaac
    10621 aggttcaaac acatcgcctt cagatggctt tgcgcactca ctttaatcag tccgaaatag
    10681 gctgcccgcg c
//
LOCUS       DAWFKN010000065        10425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFKN010000065 DAWFKN010000000
VERSION     DAWFKN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.065"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..297)
                     /locus_tag="WGR46_001614"
     CDS             complement(<1..297)
                     /locus_tag="WGR46_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0856513.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGR46_001615"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGR46_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0856514.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGR46_001616"
     CDS             complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGR46_001616"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0856515.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VNTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4882..5430)
                     /locus_tag="WGR46_001617"
     CDS             complement(4882..5430)
                     /locus_tag="WGR46_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856516.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5433..5996)
                     /locus_tag="WGR46_001618"
     CDS             complement(5433..5996)
                     /locus_tag="WGR46_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0856517.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6848..7237)
                     /locus_tag="WGR46_001619"
     CDS             complement(6848..7237)
                     /locus_tag="WGR46_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0856518.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7810..7935)
                     /locus_tag="WGR46_001620"
                     /pseudo
     CDS             complement(7810..7935)
                     /locus_tag="WGR46_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGR46_001621"
     CDS             complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGR46_001621"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0856519.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2384 a         2677 c         2653 g         2711 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacggcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcctt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgtttcaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca atctccgctt
     1381 acgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg tcataaaact gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgaaaccgc
     1621 aaaacggttt cagacggcat tttcaacctg attcaggcat cagttgcggt ttttcaaacg
     1681 cccgtgcagc tcttgaacgc tgtacacgcc cagatggtct cggcttttcg cgccttcgct
     1741 catcgcttcg ccgccggcag tagtggtgta ttgcggcaca cgttgctgca atgcgctttg
     1801 gcggatgatg tggctgtcgg acacggattg cggatcgctg gaaacggtat tcacgaccag
     1861 tgcgatttcg ccgtttttca gcgcgtcgcc gatgtgcggg cggccttcgg ggactttgtt
     1921 gatggtctgc acaattagcc cgtgttcggt caggtattgc gccgtgccgc gcgtggcgca
     1981 gatgccgtag cctaaggctt ggaagttttt ggcggttttg atgacgcgtt ctttgtcttc
     2041 ttcgcgcacg gagaggaaga ttttgccggt cgggttgagg cgttcgcctg cgccgagttg
     2101 ggctttgtag taggcttcgc cgaagcttgc gcccacgccc atgacttcgc cggtggagcg
     2161 catttccggt cccaaaatcg tgtccacgcc cgggaatttg atgaatggga acacggcttc
     2221 tttaacggca tagaaatcgg ggacaacttc tttttccacg ccttgttctt tcagggaaat
     2281 acctgccatg cagcgtgcgc cgactttggc gagcggcacg ccggtggctt tggagacgaa
     2341 gggcacggta cggctggcgc gcgggttcac ttccaacacg aacaccacgc cgtcctgcac
     2401 ggcaaactgc acgttcatca gaccgaccac gcccagcgcg tacgccatcg ctttggtttg
     2461 gcggcggatt tcgtcttgga tttcttcgct taaggagtag ggcggcagcg agcagccaga
     2521 gtcgccggag tgaatgcctg cctgttcgac gtgctgcata atgccgccga taaccacgtc
     2581 tttgccgtct gaaacgcagt ccacatccac ttcaatcgcg ttgttgagga agaaatcgag
     2641 caacacgggg ctgtcttcgg aaacctgcac ggcttcgcgc atgtattttt gcaaggcttc
     2701 ggcagagtgg acaatctgca tcgcgcgtcc gcccaaaaca taagacgggc gcacgaccag
     2761 cggatagccg atttcttcgg ctttgacgag tgcttcttct tcgttgtggg cgatgcggtt
     2821 gggcggttgg cgcagaccta agtcgttcaa cactttttgg aagcgttcgc ggtcttcggc
     2881 ggcgtcgatg ctgtcggcgg acgtgccgat gatgtttacg ccgttttcaa ccaacgcgtt
     2941 ggcgagtttc agcggggttt gaccgccgta atgcacaatc acgccccacg ggttttcggt
     3001 gcggacgatt tccaacacgt cttccaatgt cagcggctcg aaatagaggc ggtcgctggt
     3061 gtcgaagtcg gtggacacgg tttctgggtt gcagttgacc atgatggttt caaagcccga
     3121 ttcgcgcagg gcgagtgcgg cgtgaacgca gcagtagtca aactcgatgc cctgaccgat
     3181 gcggttcggg ccgccgccga gaatcatcac ttttttacgg tcggaaggac gggattcgca
     3241 ttcctcttcg taagtggagt agaggtaggc ggtttcggtg gcaaactcgg cggcacaggt
     3301 gtcgacgcgt ttgtaaaccg gatgcagctt cagcgcgtag cggtgttcgc gaacttcttt
     3361 ttcgcttacg ttcaacaatt gtgccaaacg tttgtcggag aagcctttgc gtttcagacg
     3421 gcgtagggcg gcgaaatcca aatcactcaa aatgccgtct gaaaccgctt tttcttcctt
     3481 catcaagtct tcgatttgcg ccaggaacca agggtcgatg gcgcagattt cgtggatttc
     3541 ttccagcgtg aagcccgcgc ggaacgcgtc tgccacaaac agcatacgtt cggggccggg
     3601 gtttgccagt tcgcggcgga tttccgcttt gtcggagctt ctcggattga agccgcacaa
     3661 gcctgtttcc aagccgcgca gggctttttg gaagctttcc tgaatcgtgc ggcccatcgc
     3721 catcacttcg cccacggatt tcatctgcgt ggtcaggcgg tcgtctgcgg cagggaattt
     3781 ttcaaatgcg aaacgcggga ttttggttac cacatagtcg atggaaggct cgaacgacgc
     3841 gggggttttg ccgccggtga tgtcgttgcg caactcgtcc agcgtaaagc cgaccgccag
     3901 ttttgccgcc accttcgcaa tcgggaagcc cgtcgctttg gaagccagcg cggacgaacg
     3961 gctcacgcgc gggttcatct caatcacaat catctcgccg ttttcagggt tcaccgcaaa
     4021 ctgcacgttc gagccgcccg tgttcacgcc gatttcgcgc aataccgcca acgaagcgtt
     4081 gcgcatgatt tggtattctt tgtccgtcaa cgtttgcgcc ggcgcaaccg taatcgagtc
     4141 gcccgtatgc acgcccatcg ggtcgaagtt ttcaatcgag cagatgatga tgcagttgtc
     4201 gtttttatcg cgtacgactt ccatctcgta ctctttccag ccaaggacgg actgctcaat
     4261 cagcagctcg tgcgtgggcg aggcatcgaa accgcgttcg caaatcgcca aaaactcgtc
     4321 tttattgtag gcaatgccgc cgcccgaacc gcccatggtg aaagaaggac gaatcagggt
     4381 agggaagccg acctgttctt gcgccgccaa agcttcgttc atggtgtggc agacaaaaga
     4441 tttcgggcaa gataaaccga ttttttccat cgcctcctta aagcgaccgc ggtcttctgc
     4501 tttgtcgatg gcgtcttccg tcgcaccgat caactcgaca ttgtatttcg ccagcacgcc
     4561 gttgcgcgcc aaatccagcg cacagttcag cgcggtctga ccacccatcg tgggcagaat
     4621 cgcatcgggc cgctccttgg cgataatctt ctccaccgtc tgccacataa tcggctcgat
     4681 gtaggtaaca tccgccattt cagggtcggt catgatggtg gcggggttag aattcaccag
     4741 aatgacttta tagccttctt cacgcaaagc cttgcaggcc tgtgcgcccg aatagtcaaa
     4801 ttcgcaggcc tgaccgataa cgatagggcc ggcgccgatg ataaggatgg attttaggtc
     4861 ggtacgtttg ggcatgggtg gttactcttg aattaaagaa ggttgttttg ttacatagct
     4921 tttaagaaaa gtatttccct tctctaaaat tgaagctaaa acatcatctt ctatgttaga
     4981 taaaatattc tctaaatctg cgcttgattt aattttaaag aattcttttg tttgagaatg
     5041 gtatacaaga gaaggtttat ttagctgtaa aagttctgct atattattta atgaaccaat
     5101 actcttacca tcccataaaa taaagccata atcagcaatt tctgccattt tcttatcttt
     5161 tgctgtataa aatacccttc cagttccttt gctgtctact tggacaaact gccaattccc
     5221 cacattattt cggtaaattt tgccagaaaa gtaaatatgc acattagcat aatcttgttc
     5281 ttgtagaaat ttttggatag ctttatcagc cccattagca tctccaataa caatatcaaa
     5341 attattgcta agaatattgt taattctttc tcgtatttgt ggatttagtc gtgaaatgct
     5401 gcgtgaacca gaaaagaaaa tagtgcgcat ttttatcctc tagtctttgt cattgctaaa
     5461 acaattacag atctagcatt ttttgaaaac aatttttcag ttgatatttc caatgttgct
     5521 ccgctatcaa ataaatcatc aattactaaa atattcttat tggatagatc tatattgtca
     5581 attgtgatgg aattattcag tatttctagt ttttctgatt tttcttcaat atttttgagt
     5641 ggtgtgtgtg agctagattt tcttaaaata ggagaatatg gaatattcaa gcgatcactt
     5701 agttctttgg caattaattg aactggttga ttaatacgct ctgttgtaaa aggggcggga
     5761 acaattaaat ttatagattc taaaccacta aatttttgta aaatatagtc taccagcaaa
     5821 gaaacatttt gggtttgatt acgatatttt aattgataaa cccattctcc aatcacactt
     5881 ctcttgctat caaagtgagg atgccctaat tcatcataac ctaacaaaat acttttttgc
     5941 atatgatggt ctaaagcaaa tccttttgtc cagttaccat ttagttctat cgccatttca
     6001 ttgactcctt gtcaaaatta agtataacga gcaatcctag caagccgtag cccgcatgta
     6061 gggtatgtgc ggtacgcacg cacgcgttct tcattttccc tgtaacccca acccaccgca
     6121 tgcgcgcctt gcggcacaca ctctgccgac ggattcaaag gtcgtctgaa aaaacgctcg
     6181 cggcgtttgt tacatctgcc ttgcgataaa ggctgcctga aaaattccca ctgttgatat
     6241 ttcgggcatt ctttctcaaa ccgcctgcaa ccgcaaaatc cgctgcaacc tgccccctcc
     6301 cccgtggggg agggctgggg agagggcatt ccccgaattt cggcaacctt tcccaacaac
     6361 tcaaccaccg aaatacaagc cttgtggctt gttgccctct ctctagctct ctcccacggg
     6421 agagaggacg gagtggctgt agggctaagg gttctgcaag gaaacaggtt gtgcaggttg
     6481 ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc aagtcgcttt tcaaaccgcc
     6541 tgcaaccgca aaatctgctg aaacctactc cctcccccgt gggggagggt tggggagagg
     6601 gcattccccg aatttcggca acctttccca acaactcaac caccgaaata caagccttgt
     6661 ggcttgttgc cctctctcta actctctccc acgggagaga ggactgggcg gttgttgggg
     6721 ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc agacggcctt
     6781 tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa tcaaaatcag
     6841 ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg atttctgtca
     6901 gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg gtaaagccca
     6961 agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg tgctgccctc
     7021 cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata tagtttccca
     7081 tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg tgctgccaca
     7141 attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg cgcaagatgg
     7201 ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt gttgtcgtgc tgtttgctgc
     7261 cctctctcta actctctccc acggggagag aggacggggc ggttgttggg gttaaagggg
     7321 ctgtaaggaa aacaagtcgt tcaggccact ttttactttt cagacgacct gcaatcaacg
     7381 gttgtaaatc taagttgcct ttcaaatcgc ctgcaaccgc aaaatctgct gcaaccttct
     7441 ccctcccccg tgggggaggg ctggggagag ggcattcttc gaatttcggc aaccttcccc
     7501 aacaccctaa ccgcccaaac acaagccttg ctgtttgttg ccctctctct aacgctttcc
     7561 cacagggaga caggacgggg cggctgtagt gattaagggt tttgcaagga aaacaaggaa
     7621 aacaagttgt acaggctgtt ttttattttt caagcgacct gaaatcaacg cttgtaaatc
     7681 caagtcgctt ttcaaactgc ctgcaaccgc aaaatctgct gcaacctacc ccctcccccg
     7741 tgggggaggg ctggggagag ggcattttcc aagttgcggc aatctttccc aaatccctta
     7801 gtcttctaaa atacaagcct tacggttgtt gccaaatgaa acttcttcca tacccaaaac
     7861 ttgcgtgtaa aacgcaatgg ttcggtcaat gtcggcaacg gtcagtacca gatggtcgag
     7921 tgcgctaatc ttcatttccc gctttctttt tcaggcggcg gcagtgccgt ctgaaagcca
     7981 ttatgcccgt tttgccgctt tcatattgcc aatgaatttg tcaaacaaat agccgacatc
     8041 ttgcggaccg gggctggctt cggggtggcc ttggaagcag aacacaggtt tgtcggtcag
     8101 ctcgatgcct tgcaaagtgt tgtcaaacaa ggatttgtgg gtaatgcgtg cgttagcggg
     8161 cagggtgtcg gcatcaacgg caaaaccgtg gttttggctg gtgatgacga ctttgccgct
     8221 gtccaaatct tgcacagggt ggttcgcacc gtggtggctg aagcgcattt tcagggtttt
     8281 cgcgccgata gcgaggctga tgagctggtg tcccaagcag atgccgaaaa tcggtttgcc
     8341 gctttccatc agtttttgca cggcttcgat ggcgtaggtg caaggctcgg ggtcgccggg
     8401 gccgttggac aggaacacgc cgtcgggatt gagtgccaac acgtcttgtg cgctcgtttg
     8461 ggcggggacg acggtcaggc ggcagccgcg cgaggcgagc atacgcagga tgttggtttt
     8521 cacgccgaaa tcgtaggcga cgacgtggta aggctgttcg tcaggggtaa cgaaaccctt
     8581 gcccaattcc cattcgcctt ccgtccattc gtaagtttcc gtgcaggaaa cttcttttgc
     8641 caagtctttg ccgaccatac tgccgaacgc ggcgatgagt tcttgcgctt tttcgattgt
     8701 ggcatccgca ccggtcagaa tcgcgccgcc ttgcgcgcct ttttcgcgca acagcgtggt
     8761 caggcggcgg gtatcgatgt cggcgatggc gacggttttg ttgcgtacca gatagtcgtg
     8821 caggctttcg gaggcgcgga agttgctgtg caagagcggc aggtcgcgga taatcaggcc
     8881 ggaggcataa acgctgcggc tttcttcatc ttcggcgttg gtgccggtgt tgccgatgtg
     8941 tgggtaggtg agggtaacga tttgtttgca gtaggacggg tcggtcagga tttcctgata
     9001 gccagtcatc gaagtattga acacgacttc gccggaagtc gaaccttcgt aaccgattga
     9061 tgtgccgtgg aatacgctgc cgtcagcgag gacgaggagg gcgggggtgc tcatgatggg
     9121 aatcctgttt ttaataaaat tcttgacaat gccgtctgaa gggacttcgg cagggggcat
     9181 ccggtcggaa aaatccggtc aaaaaaaaac acgccgcgca agatagacgc gtaacgtgct
     9241 tttcgggcaa gtgcctatac cctgaaagca gcatatttta agacgaaaca tacccgattt
     9301 caagcgcaaa cgcggcaggc gggctgatat gttcgcgcga aaacggaaat ccgtggcgta
     9361 aaaccgcccg ctccgcgccc actatgcccg ttacgcattt ccccacgtcc gtccggcgaa
     9421 actatgggaa tacccgaatc gtcaggaata cctgaatcgt caggaatacc cgaatcgtca
     9481 ggaatacctg aatcgtcagg aatacctgaa tcgtcaggaa tacccgaatc gtcaggaata
     9541 cccgaatcgt caggaatacc cgaaccatca ttcccgcgca ggcgggaatc cagacctgtc
     9601 ggcacagaaa cttatcgggt ataacggttt cttgagattc cgcattctag attcccgcct
     9661 gcgcgggaat gacggcatcg gtttgacggt atttaattga attgcggaaa ttgacggatt
     9721 cagtgagatt ggcgggatga agcctaccct atagcccgcc ttttatagcg gattaacaaa
     9781 aaccggtacg ggttgccccg ccccggctca aagggaacga ttccctaagg cgcccaagca
     9841 ccaggcgaac cggttccgta ccatttgtac tgcctgcggc ccgccgcctt gtcctgattt
     9901 ttgttaatcc gctatacgaa cccgccccct cccgaaaaac gcaaaaaatg ccgtccgaaa
     9961 acctttcgga cggcattttc gcgtgaaccg tcattcccac gaaaacagaa aaccaaaatc
    10021 agaaacctaa aattcgtcat tcccacgaaa gtgggaatcc aggactcagg acctgagaaa
    10081 cctttttacc cgataagttc ccgtaccgac cggtccggat tcccgcctgc gcgggaatga
    10141 cgcgtttcat ttgcccccat cccgaaaacg caaacaatac cgtctgaaag ctctgtaggg
    10201 agggcttcag cccaccgatt ccatcaatcc cacatttccg ccgttttcac cgaatccgcc
    10261 ccttttcaca atccagtcct acgcaactga accgtcattc ccgcgcaggc gggaatccgg
    10321 acttgtcggc acggaaactt atcgggtaca acggtttctt gagattttac gtcctagatt
    10381 cccgcctgcg cgggaatgac ggcggagcgg tttctgtttt ttccg
//
LOCUS       DAWFKN010000066        10199 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFKN010000066 DAWFKN010000000
VERSION     DAWFKN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.066"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(52..126)
                     /locus_tag="WGR46_001622"
     tRNA            complement(52..126)
                     /locus_tag="WGR46_001622"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:Glu,seq:ttc)
     gene            complement(144..220)
                     /locus_tag="WGR46_001623"
     tRNA            complement(144..220)
                     /locus_tag="WGR46_001623"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:Arg,seq:acg)
     gene            complement(330..2003)
                     /gene="recN"
                     /locus_tag="WGR46_001624"
     CDS             complement(330..2003)
                     /gene="recN"
                     /locus_tag="WGR46_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0856520.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEECLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2187..2789)
                     /locus_tag="WGR46_001625"
     CDS             complement(2187..2789)
                     /locus_tag="WGR46_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0856521.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2890..3831)
                     /gene="ylqF"
                     /locus_tag="WGR46_001626"
     CDS             complement(2890..3831)
                     /gene="ylqF"
                     /locus_tag="WGR46_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0856522.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3912..4766)
                     /gene="rapZ"
                     /locus_tag="WGR46_001627"
     CDS             complement(3912..4766)
                     /gene="rapZ"
                     /locus_tag="WGR46_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0856523.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4747..5709)
                     /locus_tag="WGR46_001628"
     CDS             complement(4747..5709)
                     /locus_tag="WGR46_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0856524.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5711..6160)
                     /gene="ptsN"
                     /locus_tag="WGR46_001629"
     CDS             complement(5711..6160)
                     /gene="ptsN"
                     /locus_tag="WGR46_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0856525.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6345..7235)
                     /gene="ubiA"
                     /locus_tag="WGR46_001630"
     CDS             complement(6345..7235)
                     /gene="ubiA"
                     /locus_tag="WGR46_001630"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0856526.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGALLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7265..7741)
                     /locus_tag="WGR46_001631"
     CDS             complement(7265..7741)
                     /locus_tag="WGR46_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0856527.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7756..8403)
                     /gene="bioD"
                     /locus_tag="WGR46_001632"
     CDS             complement(7756..8403)
                     /gene="bioD"
                     /locus_tag="WGR46_001632"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0856528.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8400..9701)
                     /gene="bioA"
                     /locus_tag="WGR46_001633"
     CDS             complement(8400..9701)
                     /gene="bioA"
                     /locus_tag="WGR46_001633"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0856529.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2378 a         2790 c         2568 g         2463 t
ORIGIN      
        1 gagaaatgag aaatgagaaa tgcctccgat ataatcggag gcattttgaa ttggcacgcc
       61 cacggggaat cgaaccccgg ttaccgccgt gaaagggcga tgtcctaacc gctagacgat
      121 gggcgcatat tgtattttct tactggcgca cccggagcga ttcgaacgcc cgaccctctg
      181 gttcgtagcc agatactcta tccaactgag ctacgggtgc agcacaatca cttgttcaag
      241 tgattaagaa agcgtgataa tacggattta tctgagttct gtctattgtt ttagactttt
      301 atttttagtt attttttaac tgattgattt taaaataaac tatttttcga cgcaagttgc
      361 agcaattctg ccgcatgttg ccgcgtcgta tcggtaatga cttctccgcc caacatacgg
      421 gcaacctctt cgacccgttg gattttatcc aatatactga tttcgctgac ggtttgctct
      481 ccctcgctgt gcttgcgcac ccgccagtgg ttttcgccgc aggatgcgac ttgcggaaga
      541 tgggtaacgg caagcacctg atgttttctg cccaacgaac gtaatgcctt gccgaccatt
      601 tcagccactc cccctccaat accggtatcg acctcatcaa aaatcagggt gggaacttgg
      661 gtatattggc tggcaacaac ctgtaaggca aggctgatac gcgccaattc gccgccggag
      721 gcaactttat tcagcggacg gggcggattg cctttgttgg cggcaacttg aaattgaacc
      781 tgctctaaac cgtgtgccgt cggcgacgaa ggcaacagga cgatgtcgaa acgtgcacct
      841 ttcatggcaa ggtgttgcat atgctcggtc gtttcgccgc tcaaacgctc tgccgcctga
      901 tggcgcatgg cagaaaggat gtgggcagct tcctgatatt cggcaaaatt gtgggcaaca
      961 ttatgctcga gcgcgtccaa atcggcggca gcttgcaggc tttgcaggca ctcttcgatt
     1021 tctgccaact tggcaggcaa ctcttcaggt tcgatccggt atttccgcgc catccccatc
     1081 agttcgccca tacgctgttc ctgtgccgcc aattcattgg gattgatgtc gctccaacct
     1141 gccacatcgc gcatattggc actgatttcg cccaattcgg cttcgatgct tgccaacata
     1201 ttcaggctct cggcaaagcg cggctcgatg ttttgcagat tggccaatag cttttgacat
     1261 tgatagatat ggcgttggat gccgttgtcg ccgtcaatct tgcttccgac ttcttcggca
     1321 gcctgcaaca gctcggcaga atgggcaagg ctgtcgtggc tttggctgag ggcttcccat
     1381 tcgccttgtt taatgtccaa ctgattcagt tcgttaaact gccattccag acgctcccgc
     1441 tcgataatga cggcatcggc gtgttcctgc gcctcttgga gggctttttt cgcattggcc
     1501 caattttgat aaagctgcct gacggtttcc gcctgctccc tgctgcccgc aaatgcgtcc
     1561 aacaattcgc gctgggcggc ttcctgatta agcgaatgat gggcgttttg cccgtggatg
     1621 tcgataagct ggctaccgac ggatttgagt tgcgccaagg tagcggcctg attgttgata
     1681 aagctgcggc ttttgccttt ggcatcgata atgcggcgga tactgagttc ttcttctccg
     1741 tcgtttaaaa gcccctgttc atacaattct gcttttaaaa caggcaaatg ggaaatatca
     1801 aacaacgccg acaactgcgc ttcttttgcg ccgctgcgga cttggctgta atcggcttta
     1861 tcgcccaaca gcagaccaat cgcatccaaa gtaatggact tgcccgcgcc ggtttctccg
     1921 gtcaatacgg taaagccgct ttgaaaatcc agattcagat tttcaacaat gacaaaatca
     1981 cgcaaagaaa gtgttagaag catcgtgagg attccttagt aattctaccg acaaaagcaa
     2041 tatcaacttt agatatacga attataacta agatttttta aattaaaaat aagtttttcc
     2101 ttaatttttc ttaatgcttg tttttatctg cttatttgtt gcaatgccgt ctgaagcaat
     2161 gtgcgtttca gacggcattt ggaatttcag ttgggcaggg tatcggacgg tacggcttcc
     2221 ggctcttctt catccgtagg cggcatttcc attaaatcgg gcaaaaccag ttcgcccagt
     2281 tcggtcagcg gcggaagttc ttccagaccg tccaagcgca aatcgctgag aaacgttgcc
     2341 gttgttgccc acaatgcggg ttttcccaat gtgtcccgat gtccgatgac ttcaatccac
     2401 ccccgatcct gcaaggtctg catcacgttc tgcgacaccg ccacgccgcg tatgccttcg
     2461 atgtcgccgc gcgttacggg ctgctggtag gcgataatcg ccagtgtttc catcacggcg
     2521 cgggagtagc gcggcgcacg ctgttcttgc aggctgccca gccgctcgaa tgccgtctga
     2581 acaatctgaa aacgccagcc ctcttgcgta tgcaccagtt gcaacgccct atcctgccaa
     2641 cgcgttttca actgcgccaa cacatcaatc agtttgtcct gcgacagtgg cggcacacac
     2701 aattcgcgca tagatttttc ggtcagcggt tcggtttggg tcagcagtgc ggcttcaatc
     2761 agcgcgtcgg gagaaatttt gtcggtcata cgggtatccg tgctgaaacg gcatgggttt
     2821 ggatatgccg tctgaaatcg gttggagtag agagaagctg cctgaaaaat atttttcaga
     2881 cggcattctt tatgcttccg aaggcttctg ccctttacgc tctgcttttc tggcttcgcg
     2941 tatggctttg agttcggctt ctttctgacg ggcttttttg agccaagttt cccattggtt
     3001 cggcgtttcg agggtgattc tgccgatttt gccttcacgg aagtcggtga ggatgttttc
     3061 ggcggctttt tggtagttga tccgtccacc gctgaggact gcgccgcgtt ttttggctat
     3121 ccattcgagc caaacgtttt cgtcccagtg gctgctgggg tctttgtcgg cttggtagcg
     3181 ttcttgcagc atagggaggt agtggcggcg gaggtagtct aaaagttcga gggcgacttc
     3241 ttcttcgtcc aatgcgttgc gtccgactgc gccgccggcg gcgaggttgt agccgctttc
     3301 ttcgacaatg attttcggcc acagcattcc gggggtgtcg tacagccaga agtcgtcggc
     3361 aaggaaaagg cgttgttcgg ctttggtgat gccgggttcg ttgccggttt tggcggattt
     3421 tttgcctatc atgccgttga tgagggtgga cttgccaacg ttggggatgc cgcagatgag
     3481 gacgcgcagg ggtttatcta tgccttggcg gtggggaatc atggcacgac aggcttgggt
     3541 aattttgccg tgtgcgcctg tttcggagga atcgagggcg atggcgcagg tgtcggggcg
     3601 gctgttatag tgttcgagcc agattttggt gcgctcgggg tcggcaagat cttgtttgtt
     3661 gaggatttta agtttaggtt tgcctttgga aagttgggca agcagggggt tttcgctgga
     3721 ggcgggcatt cgcgcatcca gcatttcaat caccatgtct acgcttttgg cgcgctcggc
     3781 gatggctttt ttcgctttgt tcatatggcc gggaaaccat tggattgcca tgtctttcct
     3841 ttcttttcaa tatttgaaat gccgtctgaa acggaggacg gggtttcaga cggcataatg
     3901 gtttgacgga attagcggtc tgacaggttt tgcgcctgtc tgtgccgtat cagcaattca
     3961 taacgccctt tcaaccttcg ggcgagtttt tcgacaatat agaccgaacg gtgctgtcct
     4021 cccgtgcaac cgatggcgac ggtaacgtag ctcctgcttt catcctccaa acgcggtaac
     4081 caatgcgtaa caaacctttc gatgtcgtca accatttcct gcacaagcgg ctgtccgtcc
     4141 aaataatccc aaacgggctt gtccataccg gtgtaaggcc tcaactcggg atcgtaatac
     4201 gggttgggca ggctgcgcat atcgaacata aaatccgcgt tgttcggcac accgtatttg
     4261 aacccgaagg actccaaaat caccagcagc ccggtacgtt cgaccttcag ccactgccgg
     4321 actgcatggc ggagctgttg ggcattcatc ttggaagtgt cgatacaata ggcgatttct
     4381 ttaagcggga acagccattc ccgttctttc tttaagcttt ccaacaaggt catatcctga
     4441 ttgctcagag gatgtcctcg cctggtttcg gaaaaccggc ggaccaacac gctttcttcc
     4501 gcctcgacaa acaaaacttc aaccctgtgc cccagtccgc gcagagaggc aatctgttcc
     4561 cgcgcctgtc cgatgtcaat gccggaacgc acatcgacgc tgaccgccaa ttcggtttcg
     4621 tccgcacgtt cgatatgata cgacaccagc gcgggcaaca tttccaaagg caaattgtcc
     4681 acgcagaaat aacccgaatc ttccatttgg cgcagtgcga cggacttgcc cgaaccggac
     4741 aggccgctaa tcaggacgat cttcattgtg ttgttcgttt tctttaagtt gcgtctgatg
     4801 gcgttccaaa aattcgcgcg tactgtcctt accgcgcaac tgcaaaatgt aattgcgtac
     4861 cgccgcctca accaaaacgg cgaggttgcg tccgacggcg acgggcagcg taaccgaacg
     4921 gacgttgacg ttgaggatgg attcggtttc ggtgcggatg ctcaaccggt caagctgctt
     4981 catatactcg tcgtccgcct cgactaaatt gataatgagt tgcaggattt ttttggggcg
     5041 gatggaagtt tcgccgaaaa tatggcggat attgagtatc cccaagccgc gcacttccaa
     5101 aaaatcgcgc agcataggcg aacaacgccc ttccagcgtt tccgggccga tgcggaacag
     5161 ctcgaccgca tcgtcggcaa tcaggctgtg gccgcgcgaa atcagttcca atgccaattc
     5221 gctcttaccc aggccggaat gcccggtaat cagcacgccg atttcaaaca catcgagaaa
     5281 tacgccgtgt ttgacggacg atgccgccaa ggtgcgttgc aggtaaatcc gcaacacgtc
     5341 catcagatag gggctttcga gtttggaagt cagcagtgga atatcgtttt tatgacaata
     5401 gtcgcgcagt cccggggaaa ccggcaagcc gtttgccaca atgaccaaag acatagaaat
     5461 gtcgaacagg tcgccgaact gataacccgt ttcccccgat tcgagacggt tcagatattc
     5521 cgattccgcc aaaccgacca cttggatttg gttggggtgg atgaaattca ggtgtccgac
     5581 caaagcgagg acgggcttgt ccgcctctac gccgatacgg ttgtccgcac ccgaattgcc
     5641 ggcggcccag gcaagttgta acttgtattg gttgtcgtca aacagtcggc ggacggaaat
     5701 actgggcata ttattcttca gtcaaaatgg cgcgggcttc ttccgcagaa gaaaccgtca
     5761 tcagcgattc gcggatgctt ttttgggaaa acttgccggc cagtttggat aagacttcca
     5821 aatgttcgcc agttgcgttt tccggcacca gcaagataaa aatcagggaa accggcttgc
     5881 cgtccggtgc gtcaaatccg acgggttcgc gcgtgcggat gaacgcgccc gtcgcctgct
     5941 tcacgccggc atgacggccg tgcgggatgg caacgccctg ccccaaaccg gtcgaaccga
     6001 gtttttcacg ggcaaaaaga cattcgaaaa catcagcatg ggacaatgag gattcgcgtt
     6061 ccaaaagcag gcccgcttcc tcaaacagcc tttttttact gcccacctcc atatccaaaa
     6121 caatatggga caaaggcaaa atttcgccga taaggctcat aagcttctct tttcagacat
     6181 cgcaaaacag aaagattgta ccgactgcta gggcaaatct caatcctgca tacggtacgg
     6241 gctgacataa cacagcgttt taaaaacata ttttaacgct tttcggcaca gatagaaatg
     6301 ccgtccaaag cagtttacgg ctcttcagac ggcattgccc tgccttattt cgcgaaaaac
     6361 gtatgggcaa aaatggcggt aaaccacacc caaccaattc tgttgtttgc caaaaacgtt
     6421 tcaaaacaga tttgccggac gcggcttttg atggcggcat attggcggta ttgcagcagc
     6481 aggacgatgg ggattgccgt ccaatatgcc catgccgcac cgataaccgc ccccaatact
     6541 gccatcagca gggtaaagcc gccatgacac agcataacgg cggcgatgtc gtaacgcccg
     6601 aacgtgacgg cggaggtttt gatgccgatt ttcaaatcgt cttctttgtc cgccattgca
     6661 taaaccgtgt catacgccag agtccataac acattggcgg caaagagtat ccacgcttga
     6721 ggcggcacgt ttccagcaac ggcggcaaac gccatcggga taccgaagga aaaggcaagc
     6781 ccgagataga gttggggaat cggaaaaaaa cgtttggtaa acgggtaagt cagcgcaaga
     6841 aacagcgcgg gcaggctcat cagccatgtc agatgattca gcggaatcag gcacaatgcg
     6901 gcaagcaggc acaaaaatgc cgtcagcagc agcgcttctt tttttccgac ccggccctgt
     6961 gcgaacggac ggtttttggt acgctcgaca gcaccgtcaa aatcgcggtc ggcaaaatcg
     7021 ttgatgacgc aaccggcact gcgcattaaa aacgtaccga ttgtaaacgc cgccaatacc
     7081 gccaaatcgg gaatgccgtc tgaagccagc cacaatgccc agtaggtcgg ccacagcaaa
     7141 agcagcgccc caatgggctt gtccgcccgc atcaggcgca ggtacacatc caaacggtcg
     7201 gacagtcgta aaaataaagg ggatttagga ttcatattgc cgcacagctt gaaaaaacgg
     7261 tattttatcc gataaagcgt ttcagttcgg gcagaaaata ctcggtcagc agcatttcct
     7321 cgccgtgacg ggaaaaccga gaacgccgcg cggcaaagta ccgtccgcat ccttcgccgg
     7381 aaacggcaaa ctcaaacgcc gaacgcgccc cttccaaatc ggcttgaaac agacgctcgc
     7441 ccaaagggcg cgtgccgcag tccaaaatgt tttgccaaaa cgccgaacct atacggcact
     7501 cgctccttgc ctcaacaaca gggataccgt ccagcttcaa caaaacttcg cgcaccagcc
     7561 tccctccgca ttccgtctcc aattcgccca gtttcagcag ttccaccgaa aatgtatgcg
     7621 gcaaggcgcg caatgcggcg gtcagcgacc gggctttcaa taaccggctc atcggcaggt
     7681 cgatgccgtc tgaaacggcg gcgggcaagt cgggcagcca tttcccaaat aggtgttcca
     7741 tatttttccc aatctttata ccgcgtctgt ttttgccaac tccatccatt ccgcttcgga
     7801 aaaatcggct ttcagacggc atttcaagta gctcaggctg tcttgggcga tgtgtgcgtc
     7861 gcggctgtcg tggatgtggt tgaacacgag gctgtgcaag cgaatgccat attgtttgag
     7921 cgcggcgaaa ctgagtaaag tgtggttgat actgcccagc cgtccgctgg taacgaggat
     7981 gacgggataa tcttgttgct gaatataatc aatggttaac aaatcttccg tcagcggaac
     8041 catcaatccg cccgcgcctt cgaccaaaac gacttcgtac tgcgccgcca attcttgtgt
     8101 ggcggtgcgg attttgtcca agtccaaagc cctgccgtca agacgggcag cgagatgagg
     8161 cgaagctgga taactgaaga tttcaggcat ggtcagccct tgtttgtcgg cttcctgcat
     8221 gggtttaccc atgattttac gatggacggc gatgtcgtcg gcaatgtttt ggcaaccggt
     8281 ttgcacgggc ttttgcgtaa tcacgctttt gccctgctgc aacaattgtt ttgccaacat
     8341 accggtggcg acagttttgc cgatgtccgt gtctatgccg ctgacgaagt aaacgccttt
     8401 catttgctgt gttccttcaa gatttgcacg gttttgtcgg caagtttggt caaaacgccg
     8461 tctgaaataa tatacggcgg catcagatac accagcctgc cgaacgggcg cacccaaatg
     8521 ccctgcgcca cgcaatctgc ttgaaaacgc gccatatcca cgcctttatc cagctcgatc
     8581 acgccgatgg cacccaaaac gcgtacgtct ttcacgccgc gaatatccca cgcggctttc
     8641 agacggcctt ttaagatgct ttcaatgcgg cggatatttg cctgccagtc ttgagacaaa
     8701 agcagtttga ccgaagcgca ggcaacggca cacgccagcg ggtttgccat aaacgtcggg
     8761 ccgtgcataa acacgcccgc ttcgccgcgc gaaatcgttt cggtaacttt ttgcgaagtg
     8821 attgctgccg ccagcgtcat atagccgccg ctcaaaccct tgccgatgca cataatatcc
     8881 ggcacgacct ccgcgtgttc gcaggcaaac atcttgcccg tgcgcccgaa tccggtggcg
     8941 atttcatcaa aaatcaaaac gatatcaaac tcgtcgcaca aatcgcgcaa tccgcgaaga
     9001 tactgcggat gataaaaata catgccgccc gcgccctgca cgaccggctc taaaataaag
     9061 gcggcaatat ccgcatgatg cacttcaaac aaggcgcgga caggctgcaa atccgccccg
     9121 tcccattcat cgtcgaaacg gcttttcgga ttatcgacaa aataacgctg cggcaacgcg
     9181 ctgccgaaaa tatggtgcat ccccgtttcc ggatcgcaga cggacatcgc gttccaagta
     9241 tcgccgtgat acccgcgccg taccgtcgca atattctgct tcgccgtcaa cccccgcgcc
     9301 tgctggtatt gcaccgccat cttcaacgcg acttccaccg aaaccgaacc cgaatccgca
     9361 taaaaaatac gatccagccc ctgcggcaaa atcccgacca acaatttgcc cagctccacc
     9421 gccggctcat gcgtcaaacc gccgaacatc acatgcgaca tctgtttcat ctgcgtctca
     9481 accgcctgat tcaaaacggg atgattgtaa ccgtgtatcg cacaccacca agacgacatc
     9541 ccgtcaatca gccgcgtgcc gtccgccaat tcgataaaca ccccttctgc acgtttgaca
     9601 ggataaacgg gaagcggatc ggtcatggaa gtatagggat ggagcagatg ggtacggtcg
     9661 aaatcaagca atgatgatgt gtgttgatgt tcagacggca taagtttctc tcttttctta
     9721 ttgctgcatt caaacgcaaa acgcgtattc tactccgaca gaccgcttcc cacacctttc
     9781 catccgtttc gggcgtaaaa ccacgcaaca aatcgtccgc agtataagcg cacaccgttt
     9841 cgcattcccc aagcccgatt ggaatcagac gacccaacgc ccaatattgt ttcacacagc
     9901 ctccgcccaa cgcttcaatc agtcatagac aaaacggcaa tccatacgat acagatcata
     9961 gcaacagcca tcgcaacagc gttagcaaaa tcaggggact ccgacatagg cgcatagcat
    10021 ctaccaatgc acagctcctc attcggctct atgaatacca tacccatcac aaaatccacc
    10081 gccaaaacca ggcacggctt cttatactta tgatagattt ccaccatcct gccccatata
    10141 tcccaaacat tcataccgca tatcccgcag gcaacaaatt ccgattgaag gttacagcc
//
LOCUS       DAWFKN010000067        10078 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFKN010000067 DAWFKN010000000
VERSION     DAWFKN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.067"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..330)
                     /locus_tag="WGR46_001634"
     CDS             complement(<1..330)
                     /locus_tag="WGR46_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856530.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKFNGIPKAHF
                     ELYLKGYE"
     gene            complement(636..1895)
                     /gene="rho"
                     /locus_tag="WGR46_001635"
     CDS             complement(636..1895)
                     /gene="rho"
                     /locus_tag="WGR46_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0856531.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2115..4499)
                     /gene="ppsA"
                     /locus_tag="WGR46_001636"
     CDS             complement(2115..4499)
                     /gene="ppsA"
                     /locus_tag="WGR46_001636"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0856532.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4910..5731
                     /locus_tag="WGR46_001637"
     CDS             4910..5731
                     /locus_tag="WGR46_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0856533.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5971..6633)
                     /locus_tag="WGR46_001638"
     CDS             complement(5971..6633)
                     /locus_tag="WGR46_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0856534.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6646..7473)
                     /locus_tag="WGR46_001639"
     CDS             complement(6646..7473)
                     /locus_tag="WGR46_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0856535.1"
                     /translation="MKPDIYALLELALLSDDPDEKGQLTDEAFAAVQNIDGAEANAPP
                     LDFRHAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            7750..8373
                     /gene="lolA"
                     /locus_tag="WGR46_001640"
     CDS             7750..8373
                     /gene="lolA"
                     /locus_tag="WGR46_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0856536.1"
                     /translation="MMKPHKLFQFLAVCSLTAAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKT
                     AQDRAIGGSPAAILSDKAALEAGYALEEDGSSGGIDYVLATPKKNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGSLNTKPGLSRGAFKFTPPKGVDVLSN"
     gene            8646..9785
                     /locus_tag="WGR46_001641"
     CDS             8646..9785
                     /locus_tag="WGR46_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0856537.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9917..>10078
                     /locus_tag="WGR46_001642"
     CDS             9917..>10078
                     /locus_tag="WGR46_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0856538.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT         2262 a         2557 c         2828 g         2431 t
ORIGIN      
        1 ttcgtacccc tttaaataca gctcaaaatg cgctttggga atgccgttaa acttgcgtaa
       61 atgacgtttt gcccgattcc aaaagttctc aattccatta atatggtttt gtcgttcagc
      121 aaaataactt tcatctgctt ctacttcgcc atcaaacatt tccaaatgtg gactgttttg
      181 ataaataagt aatcgtaaac gatgaaaata ataggctgcg gtacttttat taacgcctac
      241 taactctgct gctgttcttg cagttacacc tgcgacaaac agttcaatga gtttattttg
      301 tttataccgg cttagacgac tttttctcat aggggcaact ctaacttaat ttgaatttcc
      361 ctagttatct aggacagccc caaatttata caaaaatgag cgcggttcgg cgcaaccttg
      421 aatcaagttc ccgcatcggt tttcattgcc ggtacggatg cgttcaagcc ggttttgcaa
      481 aggccgcgct ttcggcaagc ggacacggac actgccgacg gttgcgccgt taacgggggg
      541 agggaggagc tgcgccgacc gtgtgaatga aagtgccgtc tgaaacccga ttttcaggct
      601 tcagacggca tttcgcatta atgcgggcgg cgcgtttatt tgccgcgcat cagttcaaag
      661 aaatcgtcgt tgtttttaga ggctttgatt ttcccgttta agaactcggt agcttcgatt
      721 tcatccatcg ggtgcaggaa cttgcgtaag agccacatac gttgcaactg gtcgttcggc
      781 acaagcagct cttcgcggcg cgtgccggat ttgttgatgt tgatggcggg gaagaggcgt
      841 ttttccgcca tacggcggtc gaggtgcaat tccatattgc cggtgccttt gaattcttcg
      901 taaatcacat cgtccatacg gctgccggtt tcaaccaatg cggtggcaat gatggtcagc
      961 gaaccgcctt cttccacgtt gcgcgctgcg ccgaagaaac gtttggggcg gtgcagcgcg
     1021 ttggcatcga caccgccggt caggattttg cccgaggtgg ggacgacggt attgtaggcg
     1081 cgggcaaggc gggtaatcga atccagcagg atgaccacgt cttttttgtg ttctaccata
     1141 cgcttggctt tttcaagcac catttcggca acttggacgt ggcgttgcgc cggttcgtca
     1201 aaggtggagg aaaccacttc gccgcggacg gaacggctca tttcggttac ttcttcggga
     1261 cgctcgtcaa tcaagaggac gatgagctcg acttcaggat agtttgcggt aacggcgtgg
     1321 gcaatatttt gcaacatcac ggttttaccg cttttgggcg gggcaaccaa gagggcgcgc
     1381 tgacctttgc cgatggggga aatcaggtcg atagcacgtc cggtcaggtt ttcttcggac
     1441 tttaagtcgc gttccagctt caactgttcg gtcggaaaca gcggggtcag gttttcaaac
     1501 aggattttat ggcggcatac ttccgggtgg tcgccgttga tggtatcaag cctgaccagg
     1561 gcaaaatagc gttcgttgtc ttttgggacg cgcacgcttc cttcgatggt gtcgcccgta
     1621 tgcaggttga agcggcggat ttgggtgggt gagacataga tgtcgtcggg accggcaaga
     1681 taggacgtgt ccgcgctgcg gaggaagccg aagccgtcgg gcaggatttc aagcgtgccg
     1741 gagcaggtga aaccctcgcc ttttttcatc atctggcgga cgatggcaaa tacgaggtct
     1801 tgtttgcgga atcggttggc gttttcgatg ccgtgttctt ccgccaattc taagagtttg
     1861 gaaatgtgca gggtttgtaa ttcggagacg tgcataataa tgatgtattt tgaagaggaa
     1921 aaagacaggc agatgccgtc tgaaagaaga agctgaccgt tgccggttgc tcggggaagg
     1981 gggaattgta ggcagtcggc gcgtgggtgt caaatattat cgcggacggg gcatcggcag
     2041 aaaatgccgt ctgagcggag ctgcttggaa aaaaataccc ccgcgctttt caggctcggg
     2101 ggtatgggca ttgattattt gttcaattca ttcgccaaat acagccaagt ttcgatgacg
     2161 gtatccgggt tcagggaaac gctttcgata ccttcctcaa ccagccattt ggcgaagtcc
     2221 ggatggtcgg acgggccttg accgcagatg ccgacatatt tgttctgctt gcggcaggcg
     2281 gagatggcaa ggtgcagcat cactttgacg gcagggttgc gttcgtcaaa cgattcggat
     2341 accaagccgc tgtcgcggtc gagaccgagg gtcagttggg tcatgtcgtt cgagccgatg
     2401 gagaagccgt cgaagtattg caggaattgt tccgccaata ccgcgttgct cggcagctcg
     2461 cacatcataa tcaggcgcag gccgtttttg ccgcgttcca agccgttttc tttcagggct
     2521 ttgacaacgg cttcggcttc gcccaaagtg cggacgaacg gaatcatgat ttcaacgttg
     2581 gtcagaccca tttcatcgcg gacgcgtttc aaggctttgc attccaaggc gaaacagtct
     2641 ttgaagttgt cggcgacata acgcgccgcg ccccggaagc ccaacatcgg gttttcttca
     2701 tgcggttcgt atacgttgcc gccgaccagg ttggcgtatt cgttggattt gaagtcggac
     2761 atacggacga tggttttacg cggataaacc gatgcggcca atgtcgccac gccttcggcg
     2821 attttatcga cgtagaagtc gacaggggac gcgtaaccgg cgatacggcg ggtaatttcc
     2881 gcttttaatt cgtcgtcttg tttgtcaaat tccaacaagg ctttcgggtg gataccgatt
     2941 tggcggttga tgataaattc catacgcgcc aagccgatgc cttcgctggg caggttggcg
     3001 aagctgaatg cgagttcggg gttgccgacg ttcatcatga ctttgacggg tgctttgggc
     3061 atattgtcca aggcgacatc ggtgatctgt acgtccaaca ggccggcata gatgaagccg
     3121 gtatcgcctt cggcgcagga tacggtcact tcctgaccgt tttccaagag ttcggtcgca
     3181 ttgccgcagc cgacgacggc aggaataccc agttcgcgcg cgatgatggc ggcgtggcag
     3241 gtacggccgc cgcggttggt cacgatggcg gaagcacgtt tcatcaccgg ttcccaatcc
     3301 gggtcggtca tatcggtaac gagtacgtcg ccggcttcga cggaatccat ctcggaagca
     3361 tctttaatca ggcgtacctt gccctgaccg actttctgac cgatggcacg gccttcgcat
     3421 aatacggttt tatcgccgtt gatggcgaag cggcgcaggt tgcggctgcc ttcttcctgg
     3481 gatttgacgg tttcggggcg ggcttgcagg atgtagagtt tgccgtccaa gccgtcgcgt
     3541 ccccactcga tgtccatcgg gcggccgtag tgtttttcga tggtcagtgc gtaatgcgcc
     3601 aactcggtga tttcttcgtc ggtaatggag aagcggttgc ggtcttcttc ggggacatcg
     3661 acgttggtta ccgatttgcc ggcttcggct ttgtcggtaa aaatcatttt gatgtgtttc
     3721 gaacccatgg ttttacgcag gatggcgggc ttgcccgctt tgagcgtggg tttgaacaca
     3781 taaaattcgt ccgggttgac cgcaccttgt acgacgtttt cgcccagacc gtaagaggag
     3841 gtaacaaaga cgacttgatc gtagccggat tcggtgtcga gggtgaacat cacacctgat
     3901 gcgccgctgt cggaacgcac catgcgttga acgccggcgg aaagggcgac gatatcgtgt
     3961 tcgaagcctt tgtggacacg gtaggaaatg gcgcggtcgt tgtacaggga ggcgaataca
     4021 tggtgcatcg cttctttaac gttttccaat ccgttgatgt tcaagaaggt ttcctgttgg
     4081 ccggcaaatg atgcgtcggg caggtcttcg gcggttgcgg aagagcgtac ggcaacggaa
     4141 atgtccgcgc cgccggcatc ggcaaccatt ttgttccatg ccgcttcgat ttcggcatcg
     4201 agctgttcgg ggaagggtgt gtccaaaatc cattggcgga tttctttgcc gacgcgtgcc
     4261 agttcggcaa cgtcttcgac atccaatttt gccagtgcgg cggaaatgcg ttcgctcaga
     4321 ccgttgtgtg cgaggaatgc gcggtaggct tcggctgtgg tggcaaagcc gccggggacg
     4381 cgaacgcctt tttcggtcag ctgactgatc atttcgccca gcgaggcgtt tttaccgccc
     4441 acgcgttcaa catctgtcat acgcaggttt tcaaaccaaa ttacgtagtt gtcggccatt
     4501 tgtgtgtcca atccaaaata tgttaaaaaa gaaacaaatc cgcgtgctta ttttaagcga
     4561 ttcgttcctc tgctgtcatg tgttttatcc gttttaaaat catgatgccg tctgaaaaat
     4621 tgcggtttcg gcgtgtgtag cggtttgaaa cttgcagccg gtatacttct ttttttgggt
     4681 attttctttg taaaacaggt ggtttgaata ggttaatgtt ttttctgttt gatttttttg
     4741 tttatttttt aaaattttct gccaaaaaat actttatata aataatttta tttcaaaatt
     4801 atattgtgtc tgtttgggtg taatccgagg taggcgtgct gcgggtgctt tccttgtgtc
     4861 tgccgctgct gttatgatga tatttttatt ctgtatttaa ggatggaaga tgagcagtcc
     4921 gcgccatgtg ttttacattt ccgaccgtac cggtctgact gccgagaata tcggcgaggc
     4981 gttgctgaac cagtttggca atctgtcgtt caaacgccat acgcatccgt ttgtcgatac
     5041 gccggaaaag gcgcgcgcgg tggtggagaa ggtcaatcgg agccggcagg aaaacggtca
     5101 gcgtccgatt gcgtttgtca gtgtggttga tgacgaaatc cgtcggatta tcaaaggggc
     5161 ggatgctttt cagattaatt tctttgagac ttttttggga ctgttggaga aggaactcaa
     5221 taccgaagcc acggcatccg agcaggggca tcacagtatc ggtaatacga agcgttacga
     5281 tgcgcgtatg gaggcggtaa atttctcatt gaaccacgat gacggggtca gcgataagaa
     5341 ccttcaggaa gcggatgtaa tcttgatggg tgtatcgcgt tcgggcaaaa cgccgacctg
     5401 cctttacctc gccctgcaat acggcatccg tgcggcaaac tatccgctga ttcccgacga
     5461 tttggaatcg gccgatctgc cgcgtatggt caagccttat agggataagc tgttcgggtt
     5521 gaccatccag ccggaacgtt tgcaggccat ccgccaagag cgccgcccga attcaactta
     5581 tgccaaaatc gatacatgcc gcagcgaggt ggcagacgcg cagagtatgt tcagacggca
     5641 tgggattccg tttgcgaata cgacggataa atcagtggaa gagcttgccg tacacatcct
     5701 tcaggcgtgc aagctcaaac gcaggttttg acgggttttg attcggtttg aaggtggaaa
     5761 tgccgtctga aatcaggttt cagacggcag ttttatagtg gattaacaaa aatcaggaca
     5821 aggcgacgaa gccgcagaca gtacaaatag tacggagccg attcacttgg tgcttcagca
     5881 ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ctgtactggt ttagatttaa
     5941 tccactatat gtttgtgggg cggatatttt tcagggctgt attttgtcca gacattcgag
     6001 cagatcgagt ggcgtgcgga tgtggaaatc cgcctgccat gagccggtat cgtcttcggg
     6061 agcgatgtag ccccattcgg cgaggacggt tttcatgccg gcgttgcggc ctgcttggat
     6121 gtcgcgttcg gcatcaccga cgtagagtgt gtgttgcggg tcggcgtgga tttgtccgca
     6181 cgcatacagc atgggtttga tgctgggctt gggttcgccg caggtgtcgc cgctgacgac
     6241 gacggcgggt gggatgatga agccgagttt ggggacgagt ttgtcggtga agcgcatggg
     6301 tttgttggtg atgatgcccc atttgatgcc gcgttggttg agttcggcaa tgagttcgtt
     6361 cacgccgtca aagagctcgg tgtcttgggc gtagcggcgg tcgtattcct ccagatattc
     6421 ggtgcgccat cgggcatagt cggggtggtc gggggtgatg cctgcgccga gtttgatcag
     6481 tcctgccgcg ccgtggctgg cttgggtgcg gatctcgtcc atgctttttg caggtagtcc
     6541 gtggcgagcg agcagggtgt tgagtgcgcc tccgaggtcg agggcggtgt cggcaagtgt
     6601 gccgtcgagg tcgaagagta cggcgtgtat catgtgtgtt ccttttcaga cggtattttt
     6661 gttctgtttc aaaccctgct cgaaatcttc cagcatatcc aattcaaagc ggctgaagcc
     6721 cgctttttcg cgcgcttcga tgttcacata gccgcggaag ataaacatat cgtaacgggt
     6781 aatcagactg cggaacaggg cgacaggttc caaaccgcgt tcgcggcaga ggtgttgata
     6841 ccaatggttg ccgatggcga catggcccac ttcgtcgcgg taaatgatgt ccaatacgcc
     6901 gcaggtttcc gaatcgccgc gctgcgccac cttcgcgcgt atccccggcg taacgtccag
     6961 cccgcgcgct tccaacacgc gcggtaccaa cgccatacgc aacagcgggt cgtaggcggt
     7021 tttgtacgcc atatcccaca aatggttgtg cgcctcgaaa ctgccgtaat cgaagccgaa
     7081 agcgcgcaat ctgtcgcgca tcaggcggaa atggtacacc tcttccttcg ccactttcac
     7141 ccagtcgcgg acaaactgaa acggcagcgt gcggaaacgg tatgccgcgt ccaaagccag
     7201 attgacggcg ttgaattcga tatgcgcaat cgcgtgcagc atcgccgcat agccttctgc
     7261 cgtgttcatt ttgcgtggcg tcagttgcga cggcgcgacc aaaacaggct tgtccggccg
     7321 tcctgcgtgg cggaagtcca gcggcggcgc gtttgcctcc gccccgtcaa tgttttgaac
     7381 ggcggcaaac gcctcatccg tcagctgtcc tttttcatcg gggtcgtctg aaagcagggc
     7441 aagttccagc aaagcataaa tatcgggttt catctcaagt ccgccgtgtt tggaaaacaa
     7501 atattatagc gtttaaaaaa aacaagatga ggcatataat ctccgcaatt cggcattccg
     7561 cgcccaaacc gtcaaataac ccggcacaca tttaaagggc ggcgtggcac gcctgccttt
     7621 tctcgggggc aaaaaaatca gccgtcggaa aacgcggttt gcaaaatgcc aaccgcccgt
     7681 cccaccgccc gtatgattgt tttgccacgc cgatacttta cgccacaccc gttccaacaa
     7741 ggaaaaataa tgatgaaacc gcacaaactg ttccaattcc tcgccgtctg ctccctgacc
     7801 gccgccgtcg cttccgcaca ggcgggcgcg gtggacgcgc tcaagcaatt caacaacgat
     7861 gcagacggta tcagcggaag cttcacccaa accgtccaaa gcaaaaagaa aacccaaacc
     7921 gcgcacggca cgttcaaaat cctgcgtccg ggccttttca aatgggaata caccaaacct
     7981 tacaggcaaa ccatcgtcgg cgacggtcaa accgtttggc tctatgatgt tgatttggcg
     8041 caagtgacca agaccgccca agaccgtgcc atcggcggca gccccgccgc catcctttcc
     8101 gacaaagccg ccctcgaggc aggttacgcc cttgaagaag acggctcgtc cggcggcatc
     8161 gactatgttt tggcaacgcc caagaaaaac aacgccggct accaatacat ccgcatcggc
     8221 ttcaaaggcg gcaacctcgc cgccatgcag ctcaaagaca gcttcggcaa ccaaacctcc
     8281 atcagcttcg gcagcctgaa caccaagccc ggcctctcgc gcggcgcgtt caagtttacc
     8341 ccgcccaaag gcgtggacgt gttgagcaac tgatgccgtc cgccccgatg ccgtctgaac
     8401 gccgccgagg cttcagacgg catttttacg caggcggaac aatgtcccgc attaccgccc
     8461 gatcgggcat cggaacggca aacccgtgaa aattaacggt tgcgccccgg ccgtttttgc
     8521 cgtttaatgc aaaccttgct gcactaaggg ccaagaaagc cgaccggccg cccccacagc
     8581 ttccgatgca ggcggcccgg ccgtccctgc aatgtttttt atttttgaac gaaaggtcga
     8641 aaaccatgaa aaaaacactg gtggcggcgg caatcctgag cctcgccttg actgcgtgcg
     8701 gcggcggaag cgataccgcc gcccaagccc cctccgccaa gcccgaagcc gaacaatcgg
     8761 gcaaactcaa tatctacaac tggtcggatt atgtcgatcc cgaaaccgtc gccgcctttg
     8821 aaaaagaaac cggcatcaag acgcgttccg attattacga cagcaacgaa acactggagg
     8881 caaaagtcct gaccggcaaa tccggctacg acctgaccgc gccgtccatc gccaacgtcg
     8941 gccggcaaat caaagcgggc gcgtatcaga aaatcgacaa ggcgcaaatt ccccattacg
     9001 gcaacatcga taaagatttg ttgaaaatga tggaagccgt cgatccgggc aacgaatacg
     9061 ccgtccccta tttctggggc atcaacacct tggcaatcaa cacgcggcag gtgcaaaagg
     9121 cattgggtac ggacaagctg cccgaaaacg aatgggattt ggtgttcaaa cccgaataca
     9181 ccgccaaact caaatcctgc ggcatcagct atttcgacag cgcaatcgaa cagattccct
     9241 tggcgttgca ctatttgggc aaagacccca acagtgaaaa tcccgaagac atcaaagccg
     9301 ccgtcgatat gatgaaagcc gtccggggcg acgtgaaacg cttcagctct tccggctata
     9361 tcgacgatat ggcggcgggc aacctgtgtg ccgccatcgg ttacggcggc gatttgaaca
     9421 ttgccaaaac ccgtgccgaa gaagccgcaa acggcgtgga aatcaaagta ttgaccccga
     9481 aaaccggcgt gggcgtgtgg gtggattcct ttatgattcc gcgcgacgca caaaacgttg
     9541 ccaacgccca ccgctatatc gactacacgc tccggcccga ggtggcggcg aaaaacggca
     9601 gcttcgttac ctacgcaccc gccagccgcc ccgcgcgcga gctgatggat gaaaaataca
     9661 cctccgatgc atcgattttc ccgaacaaag aactgatgga aaaaagtttc atcgtatcgc
     9721 ccaaatccgc agaatccgcc aaactgggcg tgaagctgtg gcaagggctc aaagcgggca
     9781 aataaccgga ctccctgccg tctgaaacct ttcgggcggc aggaaacggc gcgtccgtta
     9841 tcaaacaggg ggcgtttccc ctcctgccgg ttatgattgg gttaagatta aaatgattta
     9901 gtaaaatgag aaagatatgg atttaagtat cgtagttccc atttataatg tcgaaagtta
     9961 tttggaagcg tgtttaagtt ccatagaacc tatattaagt aatgaaaatg tcgaacttat
    10021 ccttgtgaat gacgggtcaa aagacggaag tgaagatata tgttataaat atatagat
//
LOCUS       DAWFKN010000068         9987 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFKN010000068 DAWFKN010000000
VERSION     DAWFKN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.068"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(177..1619)
                     /gene="aldA"
                     /locus_tag="WGR46_001643"
     CDS             complement(177..1619)
                     /gene="aldA"
                     /locus_tag="WGR46_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0856539.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1654..1854)
                     /locus_tag="WGR46_001644"
     CDS             complement(1654..1854)
                     /locus_tag="WGR46_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856540.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1890..2252)
                     /locus_tag="WGR46_001645"
     CDS             complement(1890..2252)
                     /locus_tag="WGR46_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856541.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISV"
     gene            complement(2461..3378)
                     /locus_tag="WGR46_001646"
     CDS             complement(2461..3378)
                     /locus_tag="WGR46_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0856542.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFVGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSVGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3550..4350
                     /locus_tag="WGR46_001647"
     CDS             3550..4350
                     /locus_tag="WGR46_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0856543.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4398..5174
                     /gene="mlaE"
                     /locus_tag="WGR46_001648"
     CDS             4398..5174
                     /gene="mlaE"
                     /locus_tag="WGR46_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0856544.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAIDFVLTAWMFTD"
     gene            5226..5720
                     /gene="mlaD"
                     /locus_tag="WGR46_001649"
     CDS             5226..5720
                     /gene="mlaD"
                     /locus_tag="WGR46_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0856545.1"
                     /translation="MKKNILEFWVGLFVLLGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5757..6347
                     /locus_tag="WGR46_001650"
     CDS             5757..6347
                     /locus_tag="WGR46_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0856546.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTTRQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6404..6682
                     /locus_tag="WGR46_001651"
     CDS             6404..6682
                     /locus_tag="WGR46_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0856547.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTGDAFERFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6679..7506
                     /locus_tag="WGR46_001652"
     CDS             6679..7506
                     /locus_tag="WGR46_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0856548.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7631..7993
                     /locus_tag="WGR46_001653"
     CDS             7631..7993
                     /locus_tag="WGR46_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0856549.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIETRFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7990..8376
                     /locus_tag="WGR46_001654"
     CDS             7990..8376
                     /locus_tag="WGR46_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0856550.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8373..8861
                     /locus_tag="WGR46_001655"
     CDS             8373..8861
                     /locus_tag="WGR46_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0856551.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8943..9110)
                     /locus_tag="WGR46_001656"
     CDS             complement(8943..9110)
                     /locus_tag="WGR46_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0856552.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9077..9457)
                     /locus_tag="WGR46_001657"
     CDS             complement(9077..9457)
                     /locus_tag="WGR46_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0856553.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9467..9593
                     /locus_tag="WGR46_001658"
                     /pseudo
     CDS             9467..9593
                     /locus_tag="WGR46_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9695..9910
                     /gene="rpmE"
                     /locus_tag="WGR46_001659"
     CDS             9695..9910
                     /gene="rpmE"
                     /locus_tag="WGR46_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0856554.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2347 a         2781 c         2478 g         2381 t
ORIGIN      
        1 agtgaatcgg ttccgtacta tctgtactgt ctgcggcttc gtttaaattt aatccgctat
       61 acaaagacaa aaacatcgac ctcggtcatg actgattgcc gatgaagcaa taaaaatgcc
      121 gtctgaacct gaaaacgggt tcagacggca ttttctatcg gggttttaaa gcggcattaa
      181 atgtcggttt ccaaataaac gacttgggtt tgcagatatt cttccaaacc gtgtttgccg
      241 tccgcgccgc cgataccgga ttttttccaa ccggcgtgga aaccctgcat cgcttcaaag
      301 ttttcgcggt tgatgtaggt ttcgccgaat tgcaggcggc gggtaacgta gaaggcttcg
      361 tttaaattag tcgtataaac agaactggtc agaccaaact cgcaatcgtt agccagtgcg
      421 atgacctggt cgagcgtgtc gaaagcggaa acgggcagca cggggccgaa ggtttcttct
      481 ttcataatgt ccatactgtt gtcggtgtcg gtcagcaggg tcggctcgaa gaaataaccg
      541 cgtccttcgg cgcgtttgcc gccgcaaacc aatttcgcac cttgtttgac tgcccgttcc
      601 actttttcgg caacggcttt gacggcgcgc tcttcaatca gcgggcccat ttccagcgcg
      661 cctgcttcgg cttcggcagg gttgccgtag cgtacgcctt tcatggcggc ggtcattttt
      721 tcaatgaatg cgtctttcag actgctgtgg acataaacgc gctcggcgca gttgcagatt
      781 tgaccagtgt tgccgacgcg cgaagccaag atggatttca ctgccaagtc caaatccgcg
      841 tctttcaaaa cgatagcagg tgctttgccg ccgagttcca gcgagacttt ggtgatgttg
      901 gcagaagcgg cttccatcac ttggcggccc gcttcaacgg agccggtcag gctgaccata
      961 tcgacttgcg gatgggcgga caaggcattg ccgatttccg cacccggacc gttcacaacg
     1021 ttgaacacgc ctgctggcag tccgaccgca tcgacgattt cggcgaagat gtggcagttg
     1081 atcggggtta cgctgctggg tttgacgacg atggtgttgc ccgttaccaa agcggggccc
     1141 attttgcggg caatcaggaa gaaggggaag ttccacggca aaatgccggc gattacgccc
     1201 agcggacgtt tgaacaataa aatattttcg cgcgggcggt cgctttggat gatttcgcct
     1261 tcgtaacgac gcgcccattc ggcttggtaa tcgagatagt cggcggtaaa catgacttcc
     1321 acgcgcgcca agtctttggt tttgccgcct tcggcaacga tggtgtcggt cagctcgtcg
     1381 gcgcgtccgc gtatgccttg ggcgattttg cgcaaatacg cgccgcgttc gaccgcaggc
     1441 aggcgttccc aagccggttg cgccgcgcgt gccgccgcca cagctcggtc aacgtctgct
     1501 tttccgcctt tgggttcgcg ggcgatggtt tcttcggtgg atgggttcaa tacgtcgcgc
     1561 cattcgccgt tgaaatcgtt ttcaaagcgt ccgttgatgt acatggccaa ttgtttcatt
     1621 tcgggttctc cagttttgta gtcagatgta gtcttagttt attcccaaat aaattgccgt
     1681 acaagtttct tttgaaatgt ttagacgaga agctactatg tctgcatatt gttctgctaa
     1741 tttatattca ctcgaatgca actgccgatt attccaatct gcattcgcat cagtaaaagc
     1801 cccagcattg ccgcctgtgg cataaccaat cgctaaactg ccaacggtat ccacaacagg
     1861 tttcaggctg cctgaatttc tctaataaat taaacactta ttctatcagc cgttaatgta
     1921 accaaacaat tatcaagata agagtagttt actaaatact catcttcttc aaattgtata
     1981 ttttgtaggg ttagatgatt attttttaat tctgccgtta tttcatactt gaaactatca
     2041 tctatttttc taaatccaat atttgaagtt gatttatcca gtatgtaatc attaaagatt
     2101 tcataggata attccacttc ataaaattga ttttcaaata ttttgaatgg gcagtatgat
     2161 ataaaacaat gtagttcata accatttatt tcaatcaata ctgcctcttc aatggtgtta
     2221 tctaactttt tgattttaca tttatataac attttatctt gcctttagat aaattgtttt
     2281 gttttttgga ttgttattca aagttctcca aaagtctttt cctgattttg tatgtatcgt
     2341 ggtaacacta ccattttcat tggttccaac aaaaataaat ttagattgac ctttggaatc
     2401 agtacccaaa aactgaacat atccagacat gcggatttcg ggttttaaat caaatgcggc
     2461 ttaccggttt tcttttttgg tttccgccgg cgtgctgccg aaatattgcc tgaatgcctg
     2521 agtaaagctg gaaacgtggc ggtagccgca taaatacgcg gtttcgccta cgcttttgcc
     2581 cccgttttgc aacagataga gcgcgtgctg catttgtttg tggtgcagcc actcgcttgc
     2641 cgtaatgccg aaatggtcgc gcatacggcg ttgcagcgtc ctttcgctga tgttcagcgc
     2701 gtctgtcagc cggttgactt ggtgtgcgcc gccgacaaac gcggcattca gggtgcggct
     2761 gaagtcttca gacggcatag cgtctgcttc cgccgtttgc cccgccgccg gcccgatgcc
     2821 gtctgaaacc gtgtcccaca agtccgacag cagccgcaaa acgtccgcct cgcggcgcaa
     2881 tgtttcgccc aaatgcccct ttgggacggc tttcaggcag gatgccgcca agccgcgcag
     2941 gttcgggggc aaatcccata tcctgaccgg ttcgcggtaa agcaggggtg cgaaacgcgc
     3001 gtattccgga cgcagcagcc attgttccat acctttaatg gtcattttga ccgttttgcc
     3061 gcctcggtaa agatagcggc tgaacaggac ttcttccccg acagcaatta ggacaatctt
     3121 gccgccgtcc gcatcgattt ggaagcggca gcggttgatg ccgaagtcca aactgccttc
     3181 cagcaagagc acgaacgaca cataaggttc ggcgaggcgg ctgctgcaaa aatcacaccg
     3241 tgccgttacc gtgccgccgt ggatggaaat gccgttggac aaagtatcga aacggtagct
     3301 gccctccaca tagctgccgc cgccgctttt gaccagattg acgaaccggc tgctgttcaa
     3361 atgttctgct ttttccatag ttttctgata aaggttatca tttgaaagat aacatttttc
     3421 gccacagcaa acaatccgcc gccacaacgg cattttcaca acacttgccg gccgtatgcc
     3481 gtctgaaaat tgcccgtctt cccgataagc cttaaaatac acgccgtcaa acctaccgtc
     3541 cgtccgtaca tgagcccatc cccctttatc gaaatgaaag acgtcgcttt tgcgtatggc
     3601 gaccgcccga ttctgaacga catcaatttc agcattccgc aaggcaattt tgccgccgtg
     3661 atgggcggtt cgggcagcgg caaaaccacg ctgatgcggc tgattacagg gcagattcgt
     3721 ccgcagtccg ggcaggtttt gattgaaggg cgggatttgt cgggcttttc ggctgacgaa
     3781 ctctacgaac accgccgccg tatgggcgta ttgttccaac acggcgcgct gtttactgat
     3841 ttgtctgtat ttgacaatat cgcttttccg atgcgcgaac tgacgcagct gccggaagcg
     3901 gtgattcgag atttggtttt gttgaaactg aacgcggtcg gtctgcgcgg tgtagaaaac
     3961 ctgatgccgt ccgagttgtc cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacgatt
     4021 gcgctcgacc ccgaaattat gttgtacgac gaaccgttta ccggtctcga tccgatttcc
     4081 ttgggcgtga ttgcccactt aatcagccgc gtcaacaagg ctttgcgttc gaccagtatt
     4141 atggtaacgc acgacattga aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg
     4201 cacggcgaaa ttatgttctc cggctcgccg caggaaatgc gcgaactgga ttcgccttgg
     4261 gtgcgccagt ttgtcggcgg cttggcagac ggtcccgtag cataccgcta tccggcgcaa
     4321 acgtcgttgc agcaggacct gttgggataa atgagttgat gccgtctgaa accgttgaac
     4381 tttacacata gaaatttatg aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta
     4441 ttcaatccct cggcagtatc acgctgtttc tgctgaacat tctggcgaag tccggtacgg
     4501 ctttcgtccg tccgcgcctg agcgtgcgcc aagtgtattt cgccggcgtg ctgtcggtgt
     4561 tgattgttgc cgtttcgggg ctgtttgtcg gtatggtctt gggtttgcag ggctatacgc
     4621 agttgtcgaa attcaaatcc gccgatattt tgggctatat ggtcgcggct tcgctgttgc
     4681 gggaactggg tccggtgttg gcggcgatcc tgtttgccag cagcgcgggc ggtgcgatga
     4741 ccagcgaaat cggtctgatg aaaacgaccg aacagctcga agcgatgaac gtgatggcgg
     4801 taaaccctgt cgcccgcgtg gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc
     4861 ttttggcttc gattttcaac gtggcgggta ttttcggcgc gtatttggtc ggtgtaacct
     4921 ggctgggctt ggacagcggc attttctggt cgcaaatgca gaacaacatc acgatacact
     4981 acgatgtaat caacggtctg atcaagtccg ccgcgttcgg cgtggcggta acgctgattg
     5041 ccgtgcatca gggcttccat tgcgtcccga cctcggaagg cattttgcgc gccagcacgc
     5101 gtacggtggt ttcgtccgcc ctgacgattt tggcgatcga ctttgtgttg accgcgtgga
     5161 tgtttacaga ttgacaggcc gtctgaaaac gaaacaaaaa acatttggat attcaaggaa
     5221 ctttaatgaa aaagaacata ttggaatttt gggtcggact gtttgtcctg ctcggcgcgg
     5281 cggcggttgc ctttctcgct ttccgcgtgg ccggcggtgc ggcgttcggc ggttcggaca
     5341 aaacttacgc cgtttatgcc gatttcggcg acatcggcgg tttgaaggtc aatgcccccg
     5401 tcaaatctgc aggcgtattg gtcgggcgcg tcggcgctat cggacttgac ccgaaatcct
     5461 atcaggcgag ggtgcgcctc gatttggacg gcaagtatca gttcagcagc gacgtttccg
     5521 cgcaaatcct gacttcggga cttttgggcg agcagtacat cgggctgcag cagggcggcg
     5581 acacggaaaa ccttgctgcc ggcgacacca tctccgtaac cagttctgca atggttctgg
     5641 aaaaccttat cggcaaattc atgacgagtt ttgccgagaa aaatgccgac ggcggcaatg
     5701 cggaaaaagc cgccgaataa acgtatgcca tcatattcaa actaggacac ttaatcatga
     5761 aaaaatcctc cttcatcagc gcattgggca tcggtatttt gagcatcggc atggcatttg
     5821 ccgcccctgc cgacgcggta aaccaaatcc gtcaaaacgc cactcaagta ttgagcatct
     5881 taaaaagcgg tgatgccaac accacccgcc aaaaagccga agcctatgcg attccctatt
     5941 tcgatttcca acgtatgacc gcattggcgg tcggcaaccc ttggcgcacc gcgtccgacg
     6001 cgcaaaaaca agcgttggcc aaagaatttc aaaccctgct gatccgcacc tattccggca
     6061 cgatgctgaa attaaaaaac gccaacgtca acgtcaaaga caatcccatc gtcaataaag
     6121 gcggcaaaga aatcatcgtc cgcgccgaag tcggcgtacc cgggcaaaaa cccgtcaaca
     6181 tggacttcac cacctaccaa agcggcggta aataccgtac ctacaacgtc gccatcgaag
     6241 gcgcgagcct ggttaccgtg taccgcaacc aattcggcga aatcatcaaa gcgaaaggcg
     6301 tggacggact gattgccgag ttgaaggcta aaaacggcag caagtaatcc ataagccatg
     6361 ccgccagtct gaaggcggca tggatttttt cctaccgaac actatgcata cagaactcaa
     6421 caacggaaca ctgcacatca gcggcgacgt aaccgtcaaa accctgaccg gggacgcatt
     6481 tgagcgtttc cggcagcaat gccgtctgaa agaaaccatt gccgtcgatt tcggcggtgt
     6541 caaacgtgcc gattccgcct gcgtgtcgct gctgctcgaa gtgctgcgcg gctgcaaagg
     6601 cagcgtcagg ctgaccggca ttcccgaatc cgtgcgcgcg ctgtccgaac tgtacgaaat
     6661 caaagactgg ctgaaatcat gaaaaaaacc gcctatgcct tcctcctgct gattgggttt
     6721 gcttccgccc ctgcatttgc cgaaacccgc cccgccgacc cttatgaagg ctacaaccgc
     6781 gccgttttca aattcaacga ccaagccgac cgctacattt tcgcccctgc cgcgcgcggc
     6841 taccgcaaag ttgcgccgaa acccgtccgc gccggcgtgt ccaatttttt taacaacctg
     6901 tgcgacgtgg tcagcttcgg cagcaatatc ttgcgcttgg acatcaaacg cgcaagcgaa
     6961 gaccttgtcc gcgtcggcat caacaccact ttcggtttgg gcgggcttat cgacatcgcc
     7021 ggcgcgggcg gcattcccga caataaaaac accttgggcg acacgtttgc ctcgtgggga
     7081 tggaaaaaca gcaattattt cgtgttgccc gtcttagggc cgtccaccgt ccgcgacgcg
     7141 ctcggcacgg gtattacctc cgtttattcg cccaagaata tcgtcttccg cacccctgtc
     7201 ggacgctggg gcacgactgc cgtatccgcc gtcagtacgc gcgaaggcct gctcgatttg
     7261 accgacagtc tggacgaagc cgccatcgac aaatacagct acacgcgcga cctctatatg
     7321 aaagtccgtg cgcggcagac cggtgcaaca cctgccgaag gtacggaaga taacatcgac
     7381 atcgacgaat tggtcgaaag tgccgaaacc ggcgcggcgg aaactgccgt tcaagaagat
     7441 tccgtatccg aaacacaggc agaagcagca ggggaagccg aaacgcaacc tggaacacaa
     7501 ccttaaagcc atgccgtctg aaaatccgtg ttcggacggc attcccttct gtcggaacgg
     7561 acagtccgaa accacagatt gggctataat cccttttttg cgcataccgc gcgacagcaa
     7621 aggaaaaacc atgtacgaag tcaaccgcag cgtattcgtc ctcattccac tcgaaccctt
     7681 ctggaactgg ctgcaaaccc tgccgggcaa ccaccttgac ggactgaccc tcgaagacat
     7741 ccaagccgac gcgaattcct accttgtccg cccgtgcgaa accgccgacg aagtatggga
     7801 cgaaatcgaa acccgttttg aagacatttt cgccgccgaa cttgccgact ggtgcgaaga
     7861 cgaacgcgag tggcctgcgc tcgatgccga cattttcaac gaatggttcg acatccagct
     7921 ttctaccgtc atcaccgacc tcgaacacga accgcttgcc cgcgaagcct tccaacccat
     7981 caacctgaac tgatgaagct gaccgtccgc aactaccatc tcgacggcta cggccatgtc
     8041 aacaatgcgc gctacctcga atttttcgaa gaagcgcgct gggcgttttt tgaagaacgc
     8101 ggactgctgc acgagttggc aggcctcata ctgattgtcg cccgcatcga catccgatac
     8161 agccgccccg ccgtcgaagg cgacgtattg cagttttcat gccgcctgaa aacccccggc
     8221 acgcgccgta ttgtgctgac ccagacgatt acactgccaa acggcaaaac cgccgcagaa
     8281 gccgacatca ccctgatgcc cgtccacgcc gccacacaac gcaccgtcag cctgcccgcc
     8341 acccttgccc gcgcattgga agccttgtcc gaatgaaaaa aatactcacc gccgccgtcg
     8401 tcgcactgat cggcatcctc cttgccatcg tcctcatccc cgacagcaaa accgcgcccg
     8461 ccttctccct gcccgacctg cacggaaaaa ccgtttccaa cgccgacctg caaggcaaag
     8521 taaccctgat taatttttgg tttccctcct gtccgggttg tgtgagcgaa atgcccaaaa
     8581 tcattaaaac ggcaaatgac tataaaaaca aaaacttcca agtccttgcc gtcgcccagc
     8641 ccatcgatcc gatagaaagc gtccgccaat atgtcaaaga ctacggtttg ccgtttaccg
     8701 tcatgtatga tgcggacaaa gctgtcggac aggcgttcgg cacacaggtt tatccgactt
     8761 ccgtccttat cggcaaaaaa ggcgaaatcc tcaaaactta tgtcggcgaa cccgatttcg
     8821 gcaaactcta ccaagaaatc gataccgcgc tggcacaata gcgatgccgt ctgaagcgca
     8881 aaaaaacaga aacgatgttc ctgttttttc agatcatccg ccccgacacg ttcagacggc
     8941 attcaacctt ccggcagatt ctttttcacc actgccggat tgcctgccgc caaagagtag
     9001 ggcggaatgt cttttgtaac caccgcgcct gcgcccacga ccgaaccgcg tccgacggtt
     9061 acgcccgcca ttacaatcac cctgtgcccc ggccagacat cgtcctccaa cgtaatcgga
     9121 cggatttccg tgtagccctc aaagcgtttt ttgaacggtc aaacttgtgg ttatttgaat
     9181 aaaacagaca ttccggctcc atcatcacat ttttgccgac caccagccca cggcagattt
     9241 cacagtttgc cccgatgccc gagccgtcgc ccaaaaccgt atccggaaac acatacgccc
     9301 cgcgttcgat attgaccccg cgtccgatat gcggagaaac ccgccgcgcc aaaaaaccgc
     9361 gcacccgtct gccgacgatg ccgatgccgc gcatatgtga cggcggcaac cccgaaccgg
     9421 caaaacgcag cagcaggaaa aagaattttg atcccatcaa acctccctca aaagaacttt
     9481 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acagatagta
     9541 cggcaaggcg aggcaacgct gtactggttt ttgttaatcc actatacatt ttatcgaata
     9601 tcctcttgac gaaacaacag aaaatagcga gaatgccgag cttgtctgta gcataggtac
     9661 attccctatg cccgcttttt tgaaaggaaa tcaaatgaaa caaggtattc acccgaacta
     9721 ccacgaagtc aacgttacct gctcttgcgg caacaaattc gcaaccaaat ccgcgatgga
     9781 aaaagaaaac ttcaatatcg aggtttgctc cctgtgccac ccgttctata ccggcaccca
     9841 aaaaatcgtc gataccaccg gccgcgtgga caaattcaac aacaaattcg gcaacctgtt
     9901 caaacgctga ttgccgcttt gcaagaatta agccctgcca tcatttcgat ggcagggctt
     9961 tttaatattt ggcacactga cttctta
//
LOCUS       DAWFKN010000069         9956 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFKN010000069 DAWFKN010000000
VERSION     DAWFKN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.069"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            54..581
                     /locus_tag="WGR46_001660"
     CDS             54..581
                     /locus_tag="WGR46_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0856555.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            669..1724
                     /gene="plsX"
                     /locus_tag="WGR46_001661"
     CDS             669..1724
                     /gene="plsX"
                     /locus_tag="WGR46_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0856556.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            1860..2111
                     /locus_tag="WGR46_001662"
     CDS             1860..2111
                     /locus_tag="WGR46_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0856557.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2362..2553
                     /locus_tag="WGR46_001663"
     CDS             2362..2553
                     /locus_tag="WGR46_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856558.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            2573..2776
                     /locus_tag="WGR46_001664"
     CDS             2573..2776
                     /locus_tag="WGR46_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856559.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            2803..3765
                     /locus_tag="WGR46_001665"
     CDS             2803..3765
                     /locus_tag="WGR46_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0856560.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3812..4183
                     /locus_tag="WGR46_001666"
     CDS             3812..4183
                     /locus_tag="WGR46_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0856561.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            4209..5135
                     /gene="fabD"
                     /locus_tag="WGR46_001667"
     CDS             4209..5135
                     /gene="fabD"
                     /locus_tag="WGR46_001667"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0856562.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            5274..7139
                     /gene="msbA"
                     /locus_tag="WGR46_001668"
     CDS             5274..7139
                     /gene="msbA"
                     /locus_tag="WGR46_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0856563.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKQVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            7239..8804
                     /gene="guaA"
                     /locus_tag="WGR46_001669"
     CDS             7239..8804
                     /gene="guaA"
                     /locus_tag="WGR46_001669"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0856564.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            8866..9612
                     /gene="fabG"
                     /locus_tag="WGR46_001670"
     CDS             8866..9612
                     /gene="fabG"
                     /locus_tag="WGR46_001670"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0856565.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         2529 a         3070 c         2377 g         1980 t
ORIGIN      
        1 ggtttttgtt aatccgctat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
       61 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
      121 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
      181 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
      241 acgacagcga caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctcgacc
      301 ccgttccaga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
      361 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
      421 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
      481 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
      541 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
      601 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
      661 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
      721 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
      781 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
      841 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
      901 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
      961 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaaa
     1021 ccattcccgg catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg
     1081 ttaccctcgc cctcgacctt ggcgcgaacg tcgactgcac gcccgaacag ctcgcccaat
     1141 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgtg
     1201 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     1261 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     1321 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     1381 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     1441 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     1501 tcgatccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa
     1561 gccacggcgg cacagacgaa accggtttcc gctacgcact cgaagaagcc taccacgaag
     1621 ccaagtccgc cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactcg
     1681 aaactgccaa agccgtccaa aacgaaaatg ccgacggtct gtaacacaca cgatgccgtc
     1741 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
     1801 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa
     1861 tgaagacatt agaaaaacgg atgaaagctc tagacaaaca gatgatgaag ttcggaaaat
     1921 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
     1981 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
     2041 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
     2101 cgtccgatta accaataaag ccaatgggtt gataaacatg aaaacatcga cggtcgtttt
     2161 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
     2221 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
     2281 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tatctatttt
     2341 gaaaaaggat attatttgcc aatgattcaa acaattttag aagacgggga tattgaagtc
     2401 aaaaacctta aaacagaaaa ttacagcggg aaggcaaggg aaatcttggg agattcctac
     2461 cctattgagc atatctctaa aaatatttct acaattcaaa atataatttc tgaatttatg
     2521 atacaaaaac aaacaccggc accaataatt tgatggaaaa ccaacaatca aaatgccgtc
     2581 tgaagcatgt ttggttttca gacggcattt ccttcgctta aaacagcgta tcggcaaccc
     2641 cgccctgcct gtccacggca atctgcatct gaaaaccatc tgtatcccaa accacacccc
     2701 catccctgtt tccgtcattt gcaccctgtc cgtattgggc aatcatcggt ttttcgctta
     2761 caatagccga atctgaacca actatccaaa aaggccgttc ccatgcagta tgccaaaatt
     2821 tccggcacgg gcagctatct tcccgccaac cgcgtcagca atgacgacct tgcccaaaaa
     2881 gtggatacct cggacgagtg gattaccgcg cgcacgggca tcaaattccg ccatattgca
     2941 gccgaaaatg agaaaaccag cgatcttgcc gccgaagcag cccgccgcgc actggatgca
     3001 gccggattag acagcggcga aatcgattta attattgtgg cgacggcaac gccggatatg
     3061 cagtttcctt ctaccgccac catcgtccaa caaaaactgg gcatcaccaa cggctgcccc
     3121 gcgtttgacg tacaggcggt gtgtgcgggc tttatgtacg cgctgaccac ggcaaacgcc
     3181 tacatcaaaa gcggcatggc aaaaaacgcg ctggtcatcg gcgcggaaac tttcagccgc
     3241 atcgtcgatt ggaacgaccg cacaacctgc gtattgttcg gcgacggcgc gggcgcggtg
     3301 gttttgagcg cggcggacaa accgggcatc atccacagca aactcaaagc cgacggcaat
     3361 tatctgaaac tcttaaacgt ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg
     3421 tacatttcga tggacggtcc cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc
     3481 gccgatgacg ttatcgaaga agccggttat accgccgctc aaatcgactg gatcgtgccg
     3541 catcaggcaa accgccgcat tatcgaatcg accgcgaaac atttaggttt gagtatggac
     3601 aaagtcgtcc tgaccgtcca agaccacggc aacacatccg ccgcatcgat tccgctggct
     3661 ttggatacgg gcatccgcag cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc
     3721 atcggcggcg gtttcgcgtg gggcgcggtg ctgttgcaat attgaacccg atgccgtctg
     3781 aaacaggctt tcagacggca tttcccatat catgaagcgg caggctttct tcaaaccaat
     3841 tgcgtgtgcg gcatttctgt ctgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta
     3901 cgcaatattg tccctctatg cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg
     3961 gggaaaacgc cgcattcccg aacaccgcct gctgctgcct gccttgttcg gcggttgggc
     4021 gggcgcatac ttgggcagca ggatattcag gcataaaacg gcgaaaaagc gttttgttgt
     4081 gctgttccgt ctgactgttt cgggcaatgt cctggcgacc tgcatcctga ttgattattt
     4141 cgttccgccc gaactttttg tcaaactcgg gcaacatctc tgacaatcca accaaacaaa
     4201 ggaataccat gtcttttgca ttcttctttc ccggacaagg ttctcaaagc ctcggcatga
     4261 tgaacggctt tacagggcag gccgtcgtca aaaacacatt tgacgaagcc tccgccatat
     4321 tggggcagga cttgtgggcg atgattaacg gcagcgatgc cgaaatcatc ggtcaaaccg
     4381 tcaataccca acccatcatg ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag
     4441 cgggtggcaa aacgcctgcc gccgttgccg gacacagcct cggcgaatat accgcgcttg
     4501 tcgccgccgg cgcattggat tttgccaacg cggtcaaact cgtgcgccta cgcgcagaac
     4561 tgatgcagtc cgccgtaccg caaggcgtgg gcgcaatggc ggcaattttg ggtttggaag
     4621 atgaacaggt aaaacaaatt tgcgccgaat ccacacaagg cgaagtggtt gaagccgtca
     4681 acttcaactc gcccggacaa gtcgtgattg caggcaatac cgccgccgtc gagcgcgcta
     4741 tgcttgccgc caaagaagcc ggcgccaaac gcgccctgcc gctgcccgtg tccgtacctt
     4801 cccattgcag cctgatgaaa cccgccgccg acaaactcgc cgaagccctg aaaaccgttg
     4861 aaatcaagca gccgcaaatc cgcgttatcc acaacgccga cgttgccgcc tacgatgatg
     4921 ccgacaaaat caaagacgcg ctcgtccgcc agctttacag ccccgtacgc tggacggaaa
     4981 ccgtcaacgc cctcgtttca gacggcattg ccgaatccgc cgaatgcggc ccgggcaaag
     5041 tgttggcggg cttggcaaaa cgcatcaaca aagccgccgc gtgcagcgca ctgaccgatg
     5101 ccggacaggt tgccgccttt atcgaaacgc actgacttcg ttccgcaaaa agcagcctgc
     5161 atgcctcagg ctgcttttca cgtccgaacg gcaaacagcc gcatatttgc gctataattg
     5221 atgacgacca aaccaccgac agcggctgcc gttgcagttc ccgccccacc gacatgatag
     5281 aaaaactgac tttcggactg tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg
     5341 cgtacgtccg cccctacaaa atccgcatcg ttgccgccct gattgccatt ttcggcgttg
     5401 ccgccaccga aagctacctt gccgccttca tcgcccccct gattaaccac ggcttttccg
     5461 cacctgccgc gccgcccgag ctgtctgccg ccgccggcat catttccacc ctgcaaaact
     5521 ggcgcgaaca gtttacctat atggtttggg ggacggaaaa caaaatctgg accgtcccgc
     5581 tctttctcat cattctcgtc gtcatccgtg gcatctgccg ctttaccagc acctatctga
     5641 tgacttgggt ctccgtgatg accatcagca aaatccgcaa agatatgttt gccaaaatgc
     5701 tgaccctttc ctcccgctac catcaggaaa cgccgtccgg caccgtactg atgaatatgc
     5761 tcaacctgac cgaacagtcg gtcagcaacg ccagcgacat cttcaccgtc ctcacgcgcg
     5821 acacgatgat tgttaccggc ctgaccatcg tcctgcttta cctcaactgg cagctcagcc
     5881 ttatcgtcgt cctgatgttc cccctgctct ccctgctctc gcgctactac cgcgaccgtc
     5941 tgaaacaagt catttccgac tcgcaaaaaa gcataggcac gatgaacaac gtgattgccg
     6001 aaacccatca gggacaccgc gtcgtcaagc tgttcaacgg gcaggcgcag gcggcaaacc
     6061 ggttcgacgc gatcaaccgc accatcgtcc gcctcagcaa aaaaatcacg caggcaacgg
     6121 cggcacattc cccgttcagc gaactaatcg cctcgatcgc cctcgccgtc gtcatcttca
     6181 tcgccctgtg gcaaagccaa aacggctaca ccaccatcgg cgaatttatg gcattcatcg
     6241 tcgcgatgct gcaaatgtac gcccccatca aaagccttgc caacattagc atccctatgc
     6301 agacgatgtt cctcgccgcc gacggcgtat gtgcatttct cgacaccccg cccgaacagg
     6361 acaaaggcac gctcgcaccg cagcgtgtcg aagggcgcat cagcttccgc aacgtcgatg
     6421 tcgaataccg ttcggacggc atcaaagccc tcgacggctt caacctcgac atcagacaag
     6481 gcgaacgcgt cgccctggtc ggacgttccg gcagcggcaa atccaccgtc gtcaacctgc
     6541 tgccccgctt tgtcgaaccg tctgccggca acatctgcat agacggtatc gacatcgccg
     6601 acatcaaact cgactgcctg cgcgcccaat ttgccctcgt ctcccaagac gtattcctgt
     6661 ttgacgacac cctgtttgaa aacgtccgat atagccgccc cgacgcgggc gaagccgaag
     6721 tcctatccgc cctccaagcc gctaacctgc aaagcctgat cgacgcatca ccgctcggac
     6781 tgcaccagcc catcggatcg aacggcagca acttatccgg cggacagcgg caacgcgttg
     6841 ccattgcccg cgccattttg aaagacgcgc cgatattatt attggacgaa gccaccagcg
     6901 cattggacaa cgaatccgaa cgcctcgtcc aacaggcgct cgaacgcctg atggaaaacc
     6961 gcaccggcat catcgtcgcc caccgcctga ccaccatcga aggggccgac cgcatcatcg
     7021 taatggacga cggcaaaatc atcgaacaag gcacacacga acaactgatg tcccaaaacg
     7081 gctactacac gatgttacgc aatatctcaa acaaagatgc cgccgtccgg acggcataaa
     7141 caaaatgccg tctgaaatgg tacaatcgcc ccgacccttt cagacggcat catatccgcc
     7201 gacccatccg attatcttca atcactgtaa aacccattat gacccaagac aaaatcctca
     7261 tccttgactt cggttcgcaa gttacccagc tcatcgcccg ccgcgtgcgc gaagcccacg
     7321 tttactgcga gctgcattcc ttcgatatgc ctttggacga aatcaaagcc ttcaacccca
     7381 aaggcatcat cctttccggc ggccctaatt ccgtttacga atccgactat caagccgata
     7441 ccggtatctt tgacttgggc attccgattt tgggcatttg ctacggcatg caatttatgg
     7501 cgcaccactt gggtggtgaa gtgcaacccg gcaaccagcg cgaattcggt tacgcgcaag
     7561 tcaaaaccat cgacagcgag ctgacacgcg gcattcaaga cgatacgcca aacacgctcg
     7621 acgtatggat gagccacggc gataaagttt ccaaactgcc taccggtttc accgtcatcg
     7681 gcgacacgcc gtcctgcccg attgccatga tggaaaacgc cgaaaaacaa ttctacggca
     7741 tccaattcca ccccgaagta acccacacca aacaaggccg cgccctgttg aaccgctttg
     7801 ttttggatat ttgcggcgca caaccgggct ggacaatgcc gaactacatc gaagaagccg
     7861 ttgccaaaat ccgcgaacaa gtcggcagcg acgaagtgat tttaggcttg tccggcggcg
     7921 tggactcttc cgtagccgcc gcgctgatcc accgcgccat cggcgaccaa ctgacctgcg
     7981 tgttcgtcga tcacggtttg ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg
     8041 cccgcaactt gggtgtgaaa gtgattcacg tcgatgccga agggcagttt atggcgaaac
     8101 tcgccggcgt taccgaccct gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag
     8161 tatttgatgc cgaagagaaa aaactcacca acgccaaatg gctggcgcaa ggcacgattt
     8221 accccgacgt aatcgaatcc gcaggcgcga aaaccaaaaa agcccacgcc atcaaatccc
     8281 accacaatgt cggcggcctg cctgaaaaca tgaagctcaa gctgcttgag ccgttgcgcg
     8341 acttgtttaa agacgaagtg cgcgaactgg gcgttgcttt gggtctgccg cgcgaaatgg
     8401 tgtaccgcca tccgttcccc ggccccggtt tgggcgtgcg catcttgggc gaagtgaaaa
     8461 aagaatacgc cgacttattg cgtcaagcag acgatatttt catccaagaa ttacgcaaca
     8521 ccaccgacga aaacggcaca tcttggtacg acctgaccag ccaggcattt gccgtattcc
     8581 tgcctgtgaa atccgtcggc gtaatgggcg acggacgcac ttacgactac gtcgtcgcac
     8641 tgcgcgcagt catcaccagc gacttcatga ccgcacactg ggcagaactg ccttactcac
     8701 tgctcggacg cgtgtccaac cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt
     8761 acgatgtcag cggcaaaccg cctgccacca tcgagtggga ataaccagca aacagggctg
     8821 caccgtccgg cgcagtcttt cgattatcgg aaaaaaggaa aaaatatgag cacacaagat
     8881 ttaagcggca aaatcgcttt ggtaaccggc gcatcgcgcg gtatcggtgc agcaattgcc
     8941 gacacactgg cggcagccgg tgccaaagtc atcggtacgg cgaccagtga gagcggtgcg
     9001 gcggcgatta gcgagcggtt ggcgcaatgg ggcggcgaag gccgcgtatt aaattccgcc
     9061 gaacccgaaa ccgtcgaaaa cctgattgcc gacatcgaaa aaacgttcgg caaactcgat
     9121 attctggtca acaacgccgg catcacccgc gacaacctcc tgatgcgcat gaaagaagaa
     9181 gagtgggacg acatcatgca ggtcaacctc aaatccgtgt tccgcgcttc taaagccgtc
     9241 ttgcgcggca tgatgaagca gcgcgccggc cgtatcatca acatcacatc cgtcgtcggc
     9301 gtgatgggca atgccggtca aaccaactat gccgcggcaa aagcaggctt aatcggtttc
     9361 tccaaatcca tggcgcgcga agtcggcagc cggggcatta ccgtcaactg cgtcgccccc
     9421 ggctttatcg ataccgatat gacccgcgcc ctgccggaag aaacccgcca agcctttacc
     9481 gcccaaaccg ccttgggcag attcggcgac gcgcaagaca ttgccgatgc ggttttgttc
     9541 ctcgcttccg atcaggcgaa atacattacc ggccagacgc tgcacgtcaa cggcggcatg
     9601 ctgatgccct gacggacagc cgtgccgaca atgccgtctg aagccctttc agacggcatt
     9661 tgcattccca aacaaaatga acacacacca caccctgccc tgcccctgcg gttcaggcac
     9721 aagctgagac ctttgcaaaa ctcctttccc tcccgacagc cgaaacccaa acacaggttt
     9781 tcgtctattt tcgccccaaa tatctcctaa ttttacccaa ataccccctt aatcctcccc
     9841 ggatacccga taatcaggca tccgggctgc cttttaggcg gcagcgggcg cacttagcct
     9901 gttggcggct ttcaacaggt tcaaacacat cgccttcaga tggctttgcg cactca
//
LOCUS       DAWFKN010000070         9795 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFKN010000070 DAWFKN010000000
VERSION     DAWFKN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.070"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(10..1305)
                     /locus_tag="WGR46_001671"
     CDS             complement(10..1305)
                     /locus_tag="WGR46_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0856566.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQSFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WGR46_001672"
     CDS             complement(1431..2318)
                     /locus_tag="WGR46_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0856567.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WGR46_001673"
     CDS             complement(2318..3283)
                     /locus_tag="WGR46_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0856568.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRADPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WGR46_001674"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WGR46_001674"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0856569.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4619..4726)
                     /locus_tag="WGR46_001675"
                     /pseudo
     CDS             complement(4619..4726)
                     /locus_tag="WGR46_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4805..5074)
                     /gene="rpsO"
                     /locus_tag="WGR46_001676"
     CDS             complement(4805..5074)
                     /gene="rpsO"
                     /locus_tag="WGR46_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0856570.1"
                     /translation="MALTVEQKAQIVKDFQRQEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5297..6232)
                     /gene="secF"
                     /locus_tag="WGR46_001677"
     CDS             complement(5297..6232)
                     /gene="secF"
                     /locus_tag="WGR46_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0856571.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVAIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(6236..8089)
                     /gene="secD"
                     /locus_tag="WGR46_001678"
     CDS             complement(6236..8089)
                     /gene="secD"
                     /locus_tag="WGR46_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0856572.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8301..8567)
                     /gene="yajC"
                     /locus_tag="WGR46_001679"
     CDS             complement(8301..8567)
                     /gene="yajC"
                     /locus_tag="WGR46_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0856573.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8695..>9795
                     /locus_tag="WGR46_001680"
     CDS             8695..>9795
                     /locus_tag="WGR46_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0856574.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRASIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2506 a         2608 c         2431 g         2250 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacgattgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcagc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgtgcata aaaaacattt gcggccaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgcgcagg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc tggaatatcg ggttcaaacc tgcataggta ttgacggcat acaccagatt
      601 tttgcattcg acgctgcctt cgggcgtgta aaccagccaa ccgttttgat gcggttcgat
      661 gcacgtcatc ggggattgct cgaaaatctg cgcaccggct tcggcagcgg cacgagcgat
      721 gcccaaagtg taattgagcg gatgcaggtg tccggataag gggtcgaatt gtgccccttg
      781 gtacatatcg ctgtcaagct gctgtttcaa ctcggcttta tcccaaagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccactgctgc aactcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcgcaa tcaatggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcctgctc
     1021 caagccgacc tgtttttcaa tttcccccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 aggtgtattt tcggacggaa tgatacaggc tgtcgggcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggtcgcca agacattcat ctgcggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgac
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc atgccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca aaatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaac ggcagggtaa
     2581 tcgtcaagaa cgacttgacc ggccccgcgc ccaagtcgga agccgcttcg agcaggcggc
     2641 tgtccagttt caccagctgc gtgtaaagcg gcagaatcat aaacggcaga taggcataaa
     2701 ccatcaccag gtttaacgaa aacgcattgt agaacaaatc taatggctca ctgataatac
     2761 ccatcttaat caataagttg tttacaatgc cgttgtgtcc gagcaaaccc atccacgcat
     2821 aaacgcgcag caggaacgat gtccaaaaag gcagcataat ggcaagcagc aaaccattac
     2881 ggacagaagg gtcggcacgg gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggcg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc acacgctgcc
     3241 ccgggcggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgctgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgaggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagcgtgga ttttccgctg ccggaactgc ccaaaagagc gaaaatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 tttgcagata aggcttggtt gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt cgcaacgtct gatgccgtct gaaactcttg tgaagcgcac gggcagcatg
     4561 aaatggaaca agattccaaa gaactttata ttatattagt ttatgcggtt ttcgggcaat
     4621 atagtggatt aaatttaaac cagtacagcg ttgccttgcc ttgccgtact atttgtactg
     4681 tctgcggctt cgtcgccttg tcctgattta aatttaatcc actataaata tggatttgag
     4741 cttgtcggaa agcaacagaa aagaaaacac cgcccatttt tctgggcggt gtcggaaagc
     4801 gtaattattt acgcagaccc aagcgggtaa tcaacgcgcg atacgtatcg ggctgggtac
     4861 ggcgcaagta ggccagcagg cggcggcgtt ggctgaccat tttcaacagg ccgcgacggc
     4921 tgtggtggtc tttggggttg gctttgaagt ggggggtcag gtcgttgatg cggaaagtca
     4981 acagagcgac ttgtacttcg gaagagccgg tgtcgccttc ttggcgttgg aaatctttaa
     5041 cgatttgtgc tttttgttct acggtcagtg ccataatgaa aactccaaaa atataagaat
     5101 ccccataggg attcagacaa gtttgccaag cctgaagaca acggcaaact ccctatgccc
     5161 aagataggac aacgtggcat tatgacacaa ttcccgcatt tctgcacaat attttaagac
     5221 cgtggaagta accgttaaaa atgccgtctg aagcattgtc tgcttgagac ggcaatgttc
     5281 agacggcata tgcgcttcaa accacgattt cttccttctg tttcgcttcc ttggcgatat
     5341 tgtcgcggct caaaccgaac attagcagaa gcgggctggc aaccaatacg gaagaataaa
     5401 tgccgaacac gatgccgatg gtcaacgcca tagaaaagcc gtgcaaggcc gcaccgccga
     5461 acaccagcat agaaacaacc atcgcctcgg tcgaaccgtg ggtaatgatg gtgcggctca
     5521 tcgttgcggt aatcgcgttg tcgatgactt ccggcacggc atgtccgcgc atcgccggct
     5581 tgcggaagtt ttcacggata cggtcgaaga cgacgacgga ttcgttcaca gaatagccca
     5641 atacggcaag gatacccgcc aagacggtca gtgaaaattc ccattggaag aaggcaaagc
     5701 agccgagaat aatcacgatg tcgtgcatat tggcgataat ggcagatacg gcaaaacgcc
     5761 attcgaaacg catcgacagg taaataatga tgccgatagc gacaaaacct aaagccatca
     5821 atccattact taccaattcc tcaccgactt gcgggccgat aaattcgact tggcgcaagg
     5881 taacgtcggg actgtctttt ttcagcaaat ccataacctg attggacaac tgtgcggaag
     5941 taacaccttc tttgttcggc aggcggatca tgatgtgttt gttcgtaccc aatgcctgaa
     6001 cctgtacatc acctattttc agcgtatcga ggcgttcgcg catcttattg acatccgcac
     6061 cctgctgata ttggacttcc attaccgtac cgccggtaaa ttcgacagag aaattcagac
     6121 ctctggtaac caaaaagaat acggcggcaa taaaggtaac caacgaaata aaggtcgtca
     6181 gtttgccgta gctcataaac ggaatatcgc gtttgatttt aaagagttcc atagcttact
     6241 ccttgcctcc tgccatttcg gctttcggct tccacaccga accaatggaa atattctgca
     6301 atttgcgtct gcgtccgtac cacagattga ccaacgcacg gaatacgacg acggacgaat
     6361 acatcgaagt cagaataccc aaacagtgta cgaccgcaaa accgcgtacc gggccggaac
     6421 cgaataccaa aagcgcgata ccggcaatca gcgaagtcag gttggaatcg acaatggtcg
     6481 cccatgcgtg ttggaaaccg agattgattg cctgctgcgg cggcacgccg gcacgcaatt
     6541 cttcgcggat acgttcgtta atcaagacgt tggagtcgat tgccataccc aaagtcaacg
     6601 ccagcgcggc catacccggt aacgtcaacg ttgcctgcat ggcagacaaa ataccgatta
     6661 ggaacagtat gttggcactc aatgcaatgg tagaaaagaa acccatcaga cgatagtaaa
     6721 ccaccatgaa tgcagcaacg atggcaaaac cccataaagt cgaatggaag cctttttcga
     6781 tgttctcctt acccaaagac ggaccgatgg tacgttcttc gacaatctgc atcggcgcgg
     6841 caagagaacc ggcacgcaac agcaaagacg tatcattggc ttcggctgtc gtcatgcttc
     6901 cggaaatttc cacgcgtccg ccggtaatgg cagtacggat aaccggcgcg gttacaacct
     6961 cggattttcc ttggtcgatc aaaaccatcg ccatgcgttt gccgacattt gcggcagtca
     7021 gttcgccaaa aatgctgcca cccgcgctgt ccaagctcag actgacggca ggtgcgccca
     7081 tttggtcgaa actcggttgc gcatcgttga tgttgtcgcc cgtcagctcg acctgtttgc
     7141 tgattagcag agtttcggga cgatctccgc cgcttgaaag cagctcataa ccgctcggca
     7201 cgttgccttc caatgcctcg cgcaacttgg caggatcgtc ctccaccata cgcaattcca
     7261 aagtcgcggt acggccgatg atgtcttttg ccttggcagt atcctgaacg cccggaagct
     7321 gcacgacgat acggtcggca ccggactgct ggatgacggg ttcggccacg cccaactcgt
     7381 tcacacggtt gtgcagggta gtgatgttct gtttgaccgc atcggaacgc actttattga
     7441 ccgcctcttc cgaaagcgtc aagacgatat tgctgccgtc tgaattcagc gttgcttcag
     7501 gaaacagctt gcgcaactgc ggcagagcct tttgcacatc acctgcatcc tgcaaaggga
     7561 cggtcaggct gtttccagcc tgacgcaccg tgccgctgcg gattttttcg cggcgcagtt
     7621 cgcggcggat gtcgcccgaa taacgttcaa acgttttctg catcgccgct ttcatatcga
     7681 cctgcatggt gaaatgcacg ccgccgcgca agtccaaacc caaaaacatc ggattggctt
     7741 tgattttcgc catccattcg gggctgtccg ccaacaggtt gagcgcggta atataccctt
     7801 cgcccaaagt gttttcgatg acgtcgcgcg ctttaagctg cgtttctgtg tctttgaaac
     7861 gcactttcag tgaattgtcc acaacaaaca tcccgtcggt ctgaatacct gcgtttttca
     7921 gcgcggcatc cactttgaat tgagtctgtt cgttgatgat gatggcttgt cggttggtcg
     7981 atacctgcac ggcgggtgtt tcgccgaata ggttgggcag cgaatacact gcggcaaccg
     8041 caatcgtgaa cacaatcagc agatatttcc ataaaggata acggttcatc attgttcctt
     8101 aatggttgga accccaccct ttcggtggtg tcggaatcgg gctatttcag aagaggcaaa
     8161 aacccttccc agccaggcaa gaccggaaag cggcatcctg aatatgccgc cctgcgtgtc
     8221 ggaacatggt caagccttcg gttggaattc aaaacaaagt gccgcattcg ggcttttccg
     8281 atgcggcttg tcggcacaaa tcaatcgact tttgcagaaa tcgcattgcg ttccacttcg
     8341 acctcgattt ttgtaccctg tccgatatcc acggtaaaaa actgttcgcc gactctggtt
     8401 accttaccct tgaaacctgc cgccaagacc actttgtcgc cgactttcaa ggcggcaagc
     8461 attgcctgat gcgctttgaa tttcttttgc tgcggacgca tgatcaggaa gtagaacacc
     8521 accataatca acactaaagg agcaaattgt gcaacagctt gattcataat ttatccgttc
     8581 tttctaatat ggttgaaaat cgagaggggt atataataac ataagaccgt aaacaatata
     8641 tcgggtttgc cgtccgtacc gaccgtatac cgcagcctgc ccgtccacaa acccatgtcc
     8701 ttgacccgcc taatcttgaa attctatgca ctattgcgcc tttttttggg caaaaacgcc
     8761 cgcaccgcat ggatttcgca tcccgcctgt gccgggcacg aacccggcgc aaaccatccc
     8821 gattcgcccg accgcatcct ctgcatcgag caggcattgc gccgcgccag tatttggcag
     8881 cacctccaaa ccatagaggc ggaagaaatc agcgatacgc gcctcgcact tgtccactcg
     8941 agcaaatatc tgaaccgttt ggaatcttgc ctgccccaaa aaggcaagat ttcccgcctg
     9001 gataacgaca ctgcaatcag cacaggctcg ctgtctgccg cacgctttgc cgccggttcg
     9061 gcagttcagg cagtcgacat ggtcatgaac cgtaaagcat ggcatgcctt ttgcgccgcc
     9121 cgcccgcccg gacaccatgc gggcagcggc aaagccggcg gattctgcct gctgaacaac
     9181 gttgccgccg gcgtcatgca tgccattgcc gaataccgcc tgaaacgcat tgccgtcatc
     9241 gatttcgatg tccactacgg cgacggtacg gcagaaatat tcaaagacga tccgcgcatc
     9301 ctgtttttca acctgtttga aaccgacctt tttcctttcc ccgaaaacaa cgatatgccc
     9361 gacggcggca atatggtgca cctgcccttg ccgccgggaa cgggcagccg cacattccgc
     9421 gaagccgtcc gcagacagtg gctgccccgg cttgccgcat tcaaacccga actggtgctg
     9481 ctgtcggcag gattcgacgc acaccgtcta gacgaatcgg gcaggctcaa cctgcacgag
     9541 gcggattttg cctggctgac acacaaaatc attcagacgg catcgggctg ccccggcaaa
     9601 atcgtatccg tactcgaagg cggctacacc cttgaacctc tagcacaatc tgctgccgag
     9661 catatccgcg tgctggcagg gctgggcaaa tccgatgccg caaccgccta tcagaaaaca
     9721 ctggacccaa caaaaaaacg gtttgccaaa ccgaaaaccg gacaggtgcg ccaacctacc
     9781 caatccgacc gttac
//
LOCUS       DAWFKN010000071         9761 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFKN010000071 DAWFKN010000000
VERSION     DAWFKN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.071"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGR46_001681"
     CDS             complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGR46_001681"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0856575.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKLEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTTLDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPTSPKQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACCGDTEAQMDMLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSDELLSHYGNVRKLWKEVFGEEAATA"
     gene            complement(3350..3541)
                     /locus_tag="WGR46_001682"
     CDS             complement(3350..3541)
                     /locus_tag="WGR46_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856576.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLETFAIT"
     gene            complement(3678..4151)
                     /locus_tag="WGR46_001683"
     CDS             complement(3678..4151)
                     /locus_tag="WGR46_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0856577.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASEMRGYSPKNWLYVLLSDVFHRKEEFDDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4204..4485)
                     /locus_tag="WGR46_001684"
     CDS             complement(4204..4485)
                     /locus_tag="WGR46_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856578.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4572..4895)
                     /locus_tag="WGR46_001685"
     CDS             complement(4572..4895)
                     /locus_tag="WGR46_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856579.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5228..6235)
                     /locus_tag="WGR46_001686"
     CDS             complement(5228..6235)
                     /locus_tag="WGR46_001686"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0856580.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6467..6703
                     /gene="acpP"
                     /locus_tag="WGR46_001687"
     CDS             6467..6703
                     /gene="acpP"
                     /locus_tag="WGR46_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0856581.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6859..8106
                     /gene="fabF"
                     /locus_tag="WGR46_001688"
     CDS             6859..8106
                     /gene="fabF"
                     /locus_tag="WGR46_001688"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0856582.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8461..9591
                     /locus_tag="WGR46_001689"
     CDS             8461..9591
                     /locus_tag="WGR46_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0856583.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGGDLERLAARDIIRFPGFVNNVSEVIKA
                     HHIFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT         2340 a         2551 c         2460 g         2410 t
ORIGIN      
        1 ccgtctaaaa agtagaatgc gctattttaa tggaaacggc ggggttttaa acggttctta
       61 tttgtttgtt ttgatttttg cttgggtaac aaatcccatc attcccacaa aaaaaatccc
      121 gtcattcccg cgaaagagga atccggtttt tgggtttcag ccattcccga taaatcgctt
      181 tagctctgcc gcaaccttaa aacggcacgg tacgaaaatg ccgtctgaaa cccagattgt
      241 caggcttcag acggcatttt ttgttcaggc ggttgccgct tcctctccga acacttcctt
      301 ccacaatttc ctgacattgc cgtaatggga cagtaattcg tcgcttactt cggttttttc
      361 cgcgtcgcgc agtttggtgt tgtgctgctg ccggcggtaa aaacggtagg cggtgcggct
      421 ttgtccggca agcgttttat caatcaaacc gcagtcggcg gcgatattca agagggcgat
      481 gttgccgtag ttgtccaaga gttgcggata ctggcgggca tgggcaagta tcagatattg
      541 gacgataaat tcaacatcga ccacgccgcc gcgtgcgtat ttgacgttgc tgtcggcagg
      601 cggatgggtg gggaacattt tttcgcgcat ttcgatgatt tcgcctgcca aggcggtttg
      661 gtcgcgttcg gcggtaagga tttcggtgcg gatgcggtcg aatgccgtct gaatttcggg
      721 tgtgccgcag atgaagcggg cgcgggtgag ggactggtgt tcccatgtcc atgcgttttc
      781 gcgctggtat ttttcgaagg cggcgatgct gtgggcgagg aagccggcgt cgccgttggg
      841 gcgcaggcgc aggtcggttt cgtagaggct gcctgcgccg gtggcggcgg aaagccagtt
      901 ggtcaggcgg cgggcgaggc ggctgtacac gtcgcctgcg tcggggtggg gatcgtcgta
      961 gagatagacg aggtcgaggt cggaggcgta gccgagttct ttgccgccga gtttgccgta
     1021 gccgatgacg gcgaattgcg gggtgtcgcg gtgttttttg ggcatatccg cccacgcgca
     1081 cgaaagggcg gcggcaatga cggtgtcggc gagggcggaa agctggtcgg agagggattc
     1141 taccgtccac aatccggcaa ggtcttggac ggcgaggcgg aagacttggg cgtgctggaa
     1201 gcggcgcagc atgtccattt gcgcttcggt gtcgccgcag caggctttga tgccgtctga
     1261 aagttcggcg gcgagtttgt cccaatcaaa cgccgtatct aataattgcg cgctgatgag
     1321 ttcgtccagc agaatcggat atttgttcag atacgccgcc acccacgagc tttggcccat
     1381 aatctctgcc agttgcgcca gggtttgcgg atgttcgttg aggaaggcga gataggcgga
     1441 tcggcggctg atgttttcga gaaaatccaa cagccgcatc aatgtatcgg tcgggttgct
     1501 ttgcgctgcc gccgcctgta cgaacagcgg cacaaccgca tcgaaacgcg gctgggcgtg
     1561 tgcggaaaga tggcggtatt tatggccgtg gcggatttgg tcgagccttg cggcgacggt
     1621 ttcggcatcg aacccgtgcg ccttcagacg gcatcgccgc ccttcttcgt cgggtttgtc
     1681 ctgccatgcc cattgccatt cgctgttgtc ttgcgtttgc tcttcgggtt cgctcaaaat
     1741 ttcgttgaac aactgattga ctttgttccg atgaacattg agaccgtctg aaaaagcgga
     1801 ataactgtcg aaacccatgc tttcggcgag cagttgccgc tgtttgggcg aggtcggcag
     1861 ggtttgggtt tgccgatcgt cccagtattg caggcggtgt tcgacatcgc gcaggaatcg
     1921 gtaggcggca agcagagttt cgacgttttc agacggcatg atcccggttt cggcaagttt
     1981 cttcagcgtt tcctgcgtgc ctttcagttg cagcgcacgc atttggccgc cgcgtatcat
     2041 ctggaaaatc tgggcgataa attcgacttc gcggatgccg cccgcgccga gtttgatgtt
     2101 gtccgccatg ccttttttgc tgacttcgct gcggatttgg cggtgcagct tacgcatcgc
     2161 ctcatacgcg ccgtaatcca gatatttgcg gaacacaaag gggcgcacca gtgctttgat
     2221 gtcgttcgga tacggcgtaa ccacgcgacc tttgcaccac gcgtagcgtt cccattctcg
     2281 cccctgtgta atcaaatatt gctccagcgc ggtttcgctc aataccaacg cgcccgaatc
     2341 gccgtccggc cgcagccgca tatcgacgcg gaacacctgc ccgtcggcgg tgatgtcgtt
     2401 cagcagcgca atcagtttct gcccgacttt ggtgaaaaac tcctgattgc cccgttcgcg
     2461 cctgccgtcg gtgtcgcccg attcgggata gacgaaaatc aaatcgatgt cggaagacac
     2521 gttcaactca tagccgcccg ccttgcccat cgccaccacg ctcaaatgct gcggcgattt
     2581 ggtataacgc ccgatcggcg tgccgtacat gtcccgataa taggcgtagg cgaaatccag
     2641 cgtggtattg acggcaaaat cggcaaacag cgtaatcgtg cgggttactt cgttcaaatc
     2701 gctgatgcgg tttatatcgc gcacgataat ctgcgacacc acataacggc gcaacccgcg
     2761 caactgccgc gccaattctt cctcgttttc ttccgcgcgg attttgtccc agtcggcaaa
     2821 ggcttggaaa tccgcttcgg tcaacacctt gtccagcatg ggcaggaaga tttcaagctt
     2881 gagtttgccg ttgtcgagct ggcgggcgag gaagagggaa tggcggcggg cggtgtcgag
     2941 gcggttgtcg gacatttcgg attccgtttg gaaggatggc aggaagatga tggcggattc
     3001 acttcaaatc aggacagggc gaggggatgc cgtaccggtt taaagtcaag ccactatatc
     3061 atagcaacct tcgatgccgt ctgaaacctt gccccgacat ccagacggat gcctgccgat
     3121 aaaaaacagc ggcaaatgcc gccgtttcct gttcacacct cgccacgctc ttttaaaata
     3181 gcataagccg agacctttgc aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg
     3241 ccaaaaaatt ttcaattgcc taaaaccttc ctaatattga gcaaaaagta ggaaaaatca
     3301 gaaaagtttt gcattttgaa aatgagattg agcataaaat tttagtaacc tatgttattg
     3361 caaaggtctc aagccgcact tttctcccgt gtggaatttt taaaaaacca tcactcttga
     3421 cgatttcatg cagctcctca cttgatttct ccgcatattc cattttcaac gccttatgct
     3481 cattgccggc ttcaatcatt ccctctccca ctgcttttgc cacaccattc caaaaaccca
     3541 taatcatgtc ctccaatcgt ttcaaataaa taaggtcgtt gtttctgtca agaaacaacg
     3601 acagtttata aactgtcgga aaacaaaatg ccgtctgaag gccggataat gcttcagacg
     3661 gcatcggatt gccggtttta accctgccct ccgccgttgt tcaaatagtg ttcccagtga
     3721 gaatataacc gggcaatatt ttcttcatag gtgtgtgcag aaggaatata accgtctttg
     3781 ggcggcatat acctgacaaa tgattctatt tcttctggat aatcaaaatc tgcataaatt
     3841 ttttcaactt cccccaaagg gtcgtcaaat tcttcctttc tatggaatac atcgcttaag
     3901 aggacgtaca gccaattttt aggggaataa cctctcattt ccgaagccaa atcttccaaa
     3961 acaggcttta attcaaaaat attggatttg ttggttaaag atagtttgaa cacacgttca
     4021 tcatgatctg aaagcgtcat ttttcgggca taagctgcca catcagccca acccaagtat
     4081 ttattcccat acccccacag aatatccttc caagaaagac ggactttttg cgcggttaaa
     4141 tcaaggttca ttttttactc cgattgcctg ccccgtcatt ccgccccttc cgattgacgc
     4201 ctgttacgaa aagtcttcca aatgacggct gattgcctct tcaacagtcg gatgccaatc
     4261 cataaatgaa gcctgcaatt cctctaaaac caactcattg aataaatcat ccaaattccg
     4321 ccaatccgtt ttttcatccg gatagatgaa atttgccata acgacagtat gtttgttttg
     4381 ggaataatcc gataaggcgc aaatcatcag ataccgcccc gaatgctcca gataaaaaat
     4441 tcgtttttcc aaaggaaacc ggttattccg ttgcccgttt tccatttttc atccttttta
     4501 acgtatcggc ttccgcgtcc ttcaaattga gttacctgcc ttccgccaag caaacgcccg
     4561 cgcccctttc atcatttgaa tatccgtttg accatattta ccagataact atcttcttta
     4621 tataaatcaa tcagcaaagg cacgataccc ggctcataaa ataattctgt cgtatcagat
     4681 gtttctttta acgcggaaat tatctccaac agttcatacc cgtatcgggg ataagcaaac
     4741 aaatagggga ctgcttcccg caaacatgaa aatgcttcgt aactttgcat ttcccatata
     4801 tgttcatctt taaaatcact caaataccat tcatccaatt cactttccgg atcggatatc
     4861 aatcggataa attcatttag ttcgtcctta aacatatcaa atccttaaat ataaaatctt
     4921 ttgattatcc gccgtatcct tattttccac atcatgaatt tcaacgccgt tcatattttg
     4981 cgttttccca agccgtccgc cacagtataa aattattttt tgtatcaatt gcttatatat
     5041 ttaacaactt accgccggac ttgctttggc tgcgtatcaa gattgtccgg ttcgcggcgg
     5101 gcgtttgctt tgtttcaaac agggcggcgc gtaagggatt ttcaaggggc gtcaggcgga
     5161 aaaaggaaat gaaaatgccg tctgaacggc aaaacgtgcg ttcagacggc attttgggcg
     5221 gatcgcgtca tcgcgccaaa gccttcaaac attctttgac caatgccgga cctttgtata
     5281 tcaatccgct gtacacctgg acggcagttg cgcccaagcg gattttctct gccgcatcct
     5341 tgccttccat aatgccgcct acgccgataa tcggcagctt gccgtctatg tggtctgcca
     5401 acagcttcaa cacccgatta cttttttcat gaacgggcag cccgctcaaa ccgccctgct
     5461 cgcctgcgag cggatggctg ccgagacttg atttgtcgat ggtggtattg gtagcgatga
     5521 tgccgtccat ttcgacggat ttgacaacgt gggcgatgtc ttcgatttgt gcttcatcca
     5581 aatcgggggc gattttgacg gcgagcggaa cgtatttccc gtatacagag gcaagctgtg
     5641 cctgtttgtt tttcaaagcc tcaagcaatg cgctcaactc gtcgccacct tgcagcgcgc
     5701 ggaggttttt ggtgttgggc gaggaaatgt tgacggtaat gtagcttgcg tgcgcgtagg
     5761 ctttttcaag gcagattaaa taatcatcgg cagcgttttc gatgggtgta accgcgtttt
     5821 taccgatgtt gatgcccaat acgccactga atttactttt ttcgatgttg cgtatcatgg
     5881 tgtcgatacc gtggttgttg aaacccatgc ggttgatgat gccttgatat tcgggaacgc
     5941 ggaagaggcg cggctgcggg ttgccgggct gcggtttggg cgttaccgtg ccgatttcga
     6001 tgaaaccaaa gccgagcgcg cccaatgcgt cgatgtattc gccgtttttg tcgagtccag
     6061 cggcaagtcc gacagggttg ggcaaatcca tacccatcaa ttttacaggt ttggtacggt
     6121 tgccggttac gggaatcaga cccaatttat aaaccgtgta gagcgcgtcg agcgtgaagt
     6181 ggtgggcttt ttcggcatcg agtgcaaaca ggatgcggcg ggcaagtgga tacatgacag
     6241 ggctttctgc ttcagatgga aatgccgcca ttttaaccga aatcccgcca aacatacggc
     6301 acacaggtca aacttgaacg acaaaacccg ccgcacccaa aaatgtacaa atcggcaaat
     6361 attgatataa tagtcattag atagaaaact ctattaaaag tcggtacaat accaccgatt
     6421 tatttccctt aaacaacctg ccgcaaggca taaaggaacg actgatatgt caaacatcga
     6481 acaacaagtt aaaaaaattg ttgctgaaca actgggcgta aacgaagccg acgtgaaaaa
     6541 cgaatcttct ttccaagacg acttgggcgc ggattctttg gacaccgtgg agctggttat
     6601 ggctttggaa gaagcattcg gctgcgaaat ccccgacgaa gatgccgaaa aaatcaccac
     6661 cgtccaactg gctatcgact acatcaatgc ccacaacggc taaccggtcg tcgcccgaca
     6721 caacagcctc tgctgcaccg cgcaatagag gcttttccct tatatggcaa actgtttgaa
     6781 cccctgccgc aaattacaga cggcatctgc acgacagccg catatgcccc cattccgaca
     6841 accaacagcg aggttatcat gagtcagaga agagtagtca ttacaggctt aggtcaggtt
     6901 tcccctgtcg gcaacactgt cgcagaggct tgggacaccc tgctcgccgg caaaagcggc
     6961 atcggcgcga ttacccgctt tgacgcatcc gacatcaaca gccgtgtcgc cggcgaagtg
     7021 cgcggtttcg acatcggaca atacatcagc gcgaaagaag cgcgccggat ggacgtattc
     7081 atccactacg gcattgccgc cgcattgcag gcaatcgccg attcgggctt ggacgatgtg
     7141 gaaaacctcg acaaagaccg catcggcgtg aacatcggtt ccggcatcgg cggactgccc
     7201 agcatcgagg tcaccggcaa agccgtaatc gaaggcggcg cgcgcaaaat caaccctttc
     7261 tttatccccg gttcgctgat caacctgatt tccggacacg ttaccatcct caaaggctac
     7321 cgcggcccga gctacgggat ggtttccgcc tgtaccaccg gcgcgcacgc catcggcaat
     7381 tccgcccgac tgatcaaata cggcgacgcg gacataatgg ttgccggcgg cgcggaaggc
     7441 gcaatcagca ctttgggcgt gggcggtttt gccgcgatga aagccctctc cacccgcaac
     7501 gacgaccccg ccaccgcttc ccgtccgtgg gacaaaggcc gcgacggctt cgttatcggc
     7561 gaaggcgcgg gcatattggt gttggaagaa ttggaacacg ccaaaaaacg cggcgcgaaa
     7621 atctacgccg aaatcgtcgg cttcggcatg agttccgatg cttaccatat caccgcgccg
     7681 aacgaagaag gccccgccct tgccgttacc cgcgcgctga aagatgccgg catcaatccc
     7741 gaagacgtgg attacgtcaa cgcgcacggc acgtccaccc ccttgggcga tgccaacgaa
     7801 accaaagccc tcaaacgcgc gttcggcgaa cacgcctaca aaaccgtcgt cagctcgacc
     7861 aaatccatga ccggccacct gctcggcgcg gcgggcggcg tggaggccgt gtacagcatt
     7921 ttggcgatac acgacggcaa aatcccgccg accatcaaca tttttgaaca agacgttgaa
     7981 gccggctgcg atttggacta ctgcgccaac gaagcgcgcg acgcggaaat cgacgttgcc
     8041 atttccaact ccttcggctt cggcggcacc aacggcacgc tggtcttcaa acgcttcaaa
     8101 ggctgattcc gcaaagtcgc cgccgacatc gaaatgccgt ctgaaaccgt ttcagacggc
     8161 attttcaaaa caccccgccc gttaccggca cttcgcggca cacaaggcag ccttataata
     8221 cgcaggcaaa cccaagccct gccggattgc gggacgaaac cctgccgtag cagaccgtat
     8281 ttccaatcat atattcaagg tgtccgcctt atgtttcaac acacaggacg acacataaag
     8341 caccgcccta tgtgtcgtcc tgatttggaa ggggttacgc cccctcccaa atagagtctg
     8401 attctaccgc cccgaaggac agatgtccga gtggcggggt tcaaccgaaa aggaaacacg
     8461 atgaaaatcg tttttatcac aacagtcgca tccagcattt acggtttccg cgcccccgtc
     8521 attaaaaaat taatcggcaa aaaccatcag gtgtatgcct ttgtatcgga gttttccgat
     8581 aatgaattgg atattatcag ggaaatgggg gttacacccg ttacctaccg ttcaaaccgc
     8641 agcgggctga acccgttttc cgatataaaa tccaccttcc tcatctttaa agaactcaaa
     8701 aaaatatcgc cggatttggt tttcccttat ttcgcaaaac ccgtgatttt cggcactttt
     8761 gccgcaaaac tggcaggcgt gcccagaatc gtcgggatgc tggaaggttt gggattcgca
     8821 tttaccccgc agccggaagg cataccgtta aaaacaaaaa tcataaaggg gattttgatt
     8881 gccttgtacc acattgccct gccgatgttg gaaagcctga ttgtattaaa ccccgacgac
     8941 aaagacgaac tgacggacaa atacggcatc aaaataaaaa acatccatat tttgggcgga
     9001 atcggtctgg attcgcggca atatccttat tccgaggcgg atattcccga tgaaaaagaa
     9061 cccgtaaaat tcctctttat cggcagattt ctgaaagaaa aggggattga tgattttatt
     9121 cgggcggcgg aacaggttaa ggacaaatac cccgatacgg tttttaccgc tttgggcgca
     9181 atcgacaaat cacgcggggg gggggggggc gatttggaac ggcttgccgc ccgcgatatt
     9241 atccgtttcc ccggttttgt gaacaatgtt tccgaagtga taaaggcgca tcatatattc
     9301 gtattgccgt cttataggga aggcgttccc cgaagcactc aggaggcaat ggccgtcggc
     9361 agggcggtga ttacgacgga tgtccccgga tgcagggaaa cggtcgccga caaggtcaac
     9421 ggcttcctga tcgagccttg gaatccccgc atcttggccg aaaaaatgat ttattttatc
     9481 gaaaacaggg aagccgtccg cctgatgggg aatgcaagtt atgcgattgc caaagataaa
     9541 ttcgatgccg aaaaagtcga tttgaaattg ctcgatattt tgaaggcgta aacaaggctg
     9601 cccgcttttg agtttcggtc atttctgata aacccccgtc attcccgcca cacccccgtc
     9661 attccctcga aagcgggaat tcaggttcgt ttagtttcgg tcatttccga taaattcttg
     9721 caactttgcg tttctagatt cccactttcg tgggaatgac g
//
LOCUS       DAWFKN010000072         9726 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFKN010000072 DAWFKN010000000
VERSION     DAWFKN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.072"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(666..1922)
                     /locus_tag="WGR46_001690"
     CDS             complement(666..1922)
                     /locus_tag="WGR46_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0856584.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(2270..2611)
                     /locus_tag="WGR46_001691"
     CDS             complement(2270..2611)
                     /locus_tag="WGR46_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856585.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2815..3672)
                     /locus_tag="WGR46_001692"
     CDS             complement(2815..3672)
                     /locus_tag="WGR46_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0856586.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3733..4548)
                     /locus_tag="WGR46_001693"
     CDS             complement(3733..4548)
                     /locus_tag="WGR46_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0856587.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4792..5577
                     /locus_tag="WGR46_001694"
     CDS             4792..5577
                     /locus_tag="WGR46_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0856588.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5836..7958
                     /locus_tag="WGR46_001695"
                     /pseudo
     CDS             5836..7958
                     /locus_tag="WGR46_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8082..9632
                     /locus_tag="WGR46_001696"
     CDS             8082..9632
                     /locus_tag="WGR46_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0856589.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVSAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEVDIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2502 a         2289 c         2532 g         2403 t
ORIGIN      
        1 gatttttgtt aatccactat aatttattga agatggctga aattcaaagt tacagaaatt
       61 tataaacaaa aaacagcaac ccgccgccgt cattcccgcg aaagcgggaa tccagtccct
      121 tgaccttaag ataatcttta attcgtcatt cccgcaaaag cgagaatccg gaagcgaaaa
      181 gtggtgggaa tgtattagaa atgactgaaa ctcaaaaaac cggattcccg cctgcgcggg
      241 aatgacgatt gatacgtttc gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
      301 ccgcagacag tacaaacagt acggaaccga ttcacttggt gcttgagcac cttagagaat
      361 cgttctcttt gagctaaggc gaggcaacgc cgtactgatt tttgttaatc cactataatt
      421 gatgaaatgc cgtctgaacg ctttgaaaag ggttcggacg gcattttgtt caaaccgaga
      481 cctttgcaaa aatagtctgt taacgaaatt tgacgcataa aaatgcgcca aaaaattttc
      541 aattgcctaa aaccttccta atattgagca aaaagtagga aaaatcagaa aagctttgca
      601 ttttgaaaat gagattgagc ataaaatttt agtaacctat gttattgcaa aggtctcaaa
      661 ccggtttatt tacgttgcaa tacggcggcg aagaagccgt cggtttggtg tcgggcggag
      721 ttgaggcgca ggtatttgcc ggtatccaaa tcgattttca aaccggcaag cagttcggcg
      781 cagttgacgg gttcaaattc gggatgttcg gacaggaaac gttcgacttg cagctcgttt
      841 tcttcgggca ggatgctgca agtggcgtac accaagcggc cttgcggttt taccagtttg
      901 gaggcggcat cgaggatgct gtgttgctgt tccaaaaggt tggccacggt ttcggcggat
      961 tggcggtatt tgagatcggg attacggcgt aaagtaccca aaccggagca gggcgcatcc
     1021 actaaaacgc ggtcggcttt gcctgccagt cgggcgatac ggctgtcgtg ttcgctgctg
     1081 atgcgttcag ggtggatgtt ggtcagtccg gcgcgggtca tacgcggttt gaggttggca
     1141 aggcgttttt cggcgatatc gaaggcgtag attctgcctt tgttcgccat ttgcgcaccg
     1201 acagccaagg ttttaccgcc ggcaccggca cagaaatcga caatgatttc gccgcgtttt
     1261 gcgcccacca ataaggcaag cagctggctg ccttcgtctt gaacttccag cgtaccgtcc
     1321 aaaaacagct cgtgtttgtt aagcgcgatt ttgtttttca ggcggatgcc ccaaggcgaa
     1381 taaggcgttg cctctgcatc ggcactttcg gcttgcaaca atggcagcac tttatcgcgt
     1441 ttgcctttca aggtattgac gcgtatatcg agcggcgcag cttggttgat gctgcggccg
     1501 aaggcgagga tttcttcttc gcgccaatgc tgctgcagct gttctaccag ccattgcggc
     1561 aattctgcgg cggtattcag gccgtctgaa aactcggttt tacgggcttt caaattgccg
     1621 agaaactcta tttcttcttc atcaagcagg tctttgattt ggctgatgtt ggtgcttctg
     1681 ccgagaacca gtgcggcgag tgcggctttg cgcggctgcg cgtgcggacg gcgcagggcg
     1741 gtactgattt tttgatagtg gcgcagcgcg gcaaaggcgg tttcggcgat ttcgtggcgg
     1801 tcttggcggc cgagcttttt gtgttcgcgg aaataggcgg agaggacggc atcggaaggc
     1861 tgtttgaaag tcagcatttc agccaaaact ttggcggtat ggtcgagttg tgcggcgttc
     1921 ataatgtaat gggttctctt actgtgggtt attcataatc aatctaacgt ccttaaaata
     1981 acaatagcat ctttatcatt acttagtatt ctatccgcaa taagaaagat gtgttaaaag
     2041 tgcgcaatcg ggatttgtct aaatttctcc gttaactaca aaggcagcct gaaaaaagtt
     2101 ctattttcag gtcgcttgaa cagcatccga tataatcaca tcagcgattt cattttgtaa
     2161 tcactcccta aaacatgagg aaaaaaacaa aatttatttt cttcgttcag tatatctatc
     2221 ctaaatatgc catcactcca ttgagttttt aatttgtatt tcgctcttgt taaatgtttt
     2281 tctgtttgcc gatttctttt gctaatgtaa aaaatactct accactctca ttggttaaag
     2341 ttttcaagta ttcatcaaca tcagtaacat taataacaac tgtttcattt gtatgtttat
     2401 taaattctaa atcaataata atattcccat taaccaatat ttcatatata tcttcttctt
     2461 cagggagcat agacaaacaa taagcacttg aagctgaaaa ctcttttttc agatactgaa
     2521 gaagaaaagt atttttaatt aaaatattat taaatttatc taatgattgc ttgacttctt
     2581 tagttgtcca atttttaaca ttattcttca ttgtattcta cctggggtcg tagttattaa
     2641 attaccatac ttatttgttt ggatcgtcat aatattagtt ggcatgttgt tgttatattt
     2701 atcatatcca atatccttat taactcggat gactctttca taaattgtat gggaaatgag
     2761 atttccatca ctaagcctct caatagatat acgcgttaat gttgggaaag ggtttcagac
     2821 ggccttcata atgcgttcga tttcgtgcca aaagccgtcc ggccgacatt ccaaaacggc
     2881 aatcaagcct tggtcgatac ggcggatttc ggttgcgttc aatgtttcgg tttcggcgac
     2941 acgcgcaggc gcgaggtagg ccatacggtc gagcagatgg tagcggactt cttggcgttt
     3001 aaacccgtat tccgcccaat cttgaatata gatgccgcgc caaccgtatg gattaagcgg
     3061 tggctcaaaa gcatggaaac cgtgtggaaa aaatccgatg ccgcgcacga tggaaacggg
     3121 ttttacgtca agcggcagac cgtgttgccg caaggttcgg atgccgtctg aagtgtgcgg
     3181 cagaccgagt tgggcggtca gtttggcggc ttttcccaac agcgtgtctt tggggtcgag
     3241 tccgcgcatg tcttcgagcc tgcccgtgcc gccgccgtaa tatttgcagg tcagctcgat
     3301 atggtagggc ttgccgttaa gccttaccac aaaatccgcc gcgccttgcg tattgccgtc
     3361 cgaaccggaa accgtgaggt tgtgcgcgag cagttcggcg tgtggcgcgt tggcaaacca
     3421 aaatgccagc agttcttcgg cgtaaatgcc gaggcggtgt ccgaacgggg cgcgtcgggt
     3481 gaggtgttcc gtcagcggcg cgggatcggc atccaatgcc aaaagatagc ggaaaccgcg
     3541 ttcccccaat agttcgcgca cgcccaattc gcaaccgctt tgccacaaag gcggcgcagt
     3601 cagcagcgag gcgaggtcgc ggacgggttg gctggtgagc ttccaccata atgcgtctag
     3661 ggcgtaattc atgcttatcc gaatatcagt catgccgtct gaaaagctgg aaatgttttc
     3721 agacggcctt aatcagtctt ttttatggat acgatggctg acggtattga aaaaaccgcg
     3781 caggatatcg atgtcttcgg tttgcgtgtt ggcgcgtccg aacaggctct gcatacggcg
     3841 catcagacgc tcgccgttgc ggcggttgaa aaagccgatg tcgttcatca cgctttccat
     3901 gtgggcgacc atgcctttga tttgctcgtg ggtcgcagcg tggtcttctt gttgaagatg
     3961 ggtcatgggc gaaccggttt ggctgaagat ttcgtagcac acgacctgca cggcttgggc
     4021 gaggttgagc gagaaatagt cgggattgcc gttgatggtc atcagtcggt tgcaggcttg
     4081 gacttcttcg atgctcaagc cgaaagtctc gttgccgaaa accagtgcca ctttctcgcc
     4141 tcggtttgcg gtctgcagta attcggatac caaatcgcgc ggggtttgca gcggcgcagt
     4201 aatttcgcgg cggcggctgg tcagggcgca ggcgatggtg gtgtcggcaa gggcttcgtc
     4261 caaagaagcg gcaatggtgg cattttccaa aacgtctgcc gcgccggaag cgaggatgaa
     4321 gctttcttcc ggtaatttaa acgattgagg atgctccggg tcaaacacgg gcgggttttc
     4381 cgtcatcggc gttgccatca gatttggggc gacgatggtc agtttgtgca gacccattgt
     4441 tttcatcgcg cgcgcggcag agccgatgtt ggcgggatgg ctggtgcgcg tgaggatgat
     4501 gcggatgttg tccagataag cgggcagggc gggtttgaga gtagtcatgt tcggttttcg
     4561 gtcggaatgg aatagggtat aatgcgccgt ctttttcgtt cggacggtca aatgccgtct
     4621 gaaatgttct ttaacaacgt cggaaattta acgttgcgcc tgaagcagac acgcgtcagg
     4681 cggtatttta cccgatttgg caggatttac cccaaccaaa taaactccga ttttgccgct
     4741 ccgaaggact gtacgtccga accggcggta tgccaccctt aaggaaatct gatgaatccg
     4801 tttttgaata cagcctttaa agccgcccgc cgtgccggtc agatgatgat acgcgccgcg
     4861 ggcaatctcg atgccgtcaa aaccgacagc aaagccttca acgattttgt ttccgatgtt
     4921 gaccgcaatt ccgaaatcat cctggttgag gctttgaaag aagcctatcc gcaccacaaa
     4981 atcacttgtg aagaaagcgg ctcccacggc aaagccgcag ccgagtacga atggattatc
     5041 gatccgctcg acggcacgac caatttcctt cacggtcatc cccaatacgc catctctatg
     5101 gcgctgctgc acaaaggcgt gttgcaagaa gctttggttt acgcccccga acgcaacgac
     5161 gtatatatgg cttcgcgcgg caaaggcgcg ttgctcaacg accgccgcat ccgcgtttcc
     5221 aaccgcatcg aattgaaccg ctgcctgatc ggtaccggct tccctgttgt cgatcaaagc
     5281 atgatggaca agtatctggt gattttgaaa gatttcttgg ccaaaaccgc cggcggccgt
     5341 cgtgaaggcg cggcttcttt ggatttgtgt gctgtagcaa caggtcgttt tgacggcttt
     5401 ttcgagttta acctcaaacc gtgggacatt gccgcaggtg cattgattgt tcaggaagcg
     5461 ggcggtatcg taaccgatat gtcgggtgaa gacggctggc tggaaagcgg cgatattgtg
     5521 gctgccaatc ccaaagtgct ggcgcaaatg ttgaaaatca tttccgcaca cgtttaaatt
     5581 tcagcccgct cccgggaatg cgggaaaacg ggcgggtatg ccgtctgaag tatcgcgccg
     5641 atatttcaga cggcattttt actggcagcg ggggcaggaa ggtggaaacg gctaaatgcc
     5701 cgaaactgcc gaggatggaa actaaacgga gaaccctgtg caatttgttt atagtggatt
     5761 aacaaaaacc agtacggtgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
     5821 tgaagcacca agtgaatggg caaagccaac gcctttaccg cgccgattac cgtcgtcaat
     5881 tacgacgaac aagccctaaa caacaccgaa gcgcgtactt tggtggatgc cgtagcgaaa
     5941 aaagacgctt ccgtttggca gttcggcggc gaaagcaaca cgctgaccgg actgtatttc
     6001 agaggttatc agcttgactc gcgccaattc agcgtcaacg gtttggcagg tatgtacggc
     6061 acgcaaggca cggccagcgt gcaagttgga tcggcacaac tgattaaagg cgcatccact
     6121 gccgtaaacg gcatggaccc tgaaggtgcg gtatccggtt ccgtcaatat cgaaaccaaa
     6181 aaagccgccg atgaaggcaa ccgcaaaatc ggtttgggct ggttcagtaa caaccgcgcc
     6241 caaggtacgt tcgacttggg ccaacgtttc ggcgaaaaca aagcattcgg cgtgcgcgcc
     6301 aacggcaaac tgcgccacgg cgacaccccg cgccacggtt acagcgaaga caacaaagaa
     6361 tttgcggtaa atgccgacta tcgcggcgaa acactgcgcg tagcgttcga ttccatctac
     6421 gcaaaacgca aaaccaacgg cggtcgcgcg cgtatgcagg acattcaaaa cgccggcgga
     6481 cgcttgtttg gcgcgcccga tggtaaaacc aacctgctgc cttcttggaa ctggcaaaat
     6541 accgccggcc aaaccaatat gcttacgttt gaatgggatg cgtttgataa tgcacagatt
     6601 acaggcggta tcggctacaa caaggcacgc tattacggca cgctgatttc cccgaccgta
     6661 tgtttgaatg ctaccagcac ttgtaccgat atgcaaaaca caaacgccag cggcaaagta
     6721 acggcacgca actatgacta caataccggt actgcgcgcc tgaccgacca atatttccgc
     6781 acattgagta tgaacctgac cgcacgcggc gaatttgaaa ccggtccggt aacacataac
     6841 tggagcaccg cgttcgaccg tgttatccgc cagcggaaaa ccataaacgg cagcggaaac
     6901 ggcaacagca agatagaagt gaaggcaaac gaaaatattg cgaatcaatt ggcatcgttc
     6961 aaagcctact atcccaattc ttgggcgaaa accgccaact tggatgccaa tatcaaagtt
     7021 aacagcctgg ctttgtccga caccttgggc ttcgttgata acaaataccg cctgactttg
     7081 ggcggacgtt tccaagcggt tgaatacacc gataagaaaa aatcacagag cggcgatgcc
     7141 aaacgtttca gcccgatgct gatggcggcg tgggtgccgc aacctgattt ggttgtgtac
     7201 ggcaactata tggaagactt ggagcctgcc gacatcaaaa ccgatgattc cggcgaaacg
     7261 acaatggcga aaccgcgtgt cagccgccag tttgaagtgg gcgtgcgtaa aaactggggc
     7321 aatttcgtaa ccacgttaaa cgcgttccaa atcaaacgcc cggctactgg cgcggtcata
     7381 caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac aattccgatt
     7441 tcgcccgcta taaggcgcaa ggcggcgcgg ccggcgacga gcaaggtatg gaacgcaatc
     7501 gcggtattga gttcaacacc tacgcgaact tgctcaacaa tactttgcgt ccgactttgg
     7561 gtctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat atgctggtta
     7621 acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac
     7681 cgtttgccaa aggattgact ttaaacggta atgtttcgta tttcggcaag tcttaccaag
     7741 acacgcaaaa acaatacgcc ttcccgtcct acaccttgat tgacgtaggc gcgcgctaca
     7801 aaaccaagct cggcaaaaat actttgaccg tcagcagctc ggtggaaaac ctgttcaaca
     7861 aaaactattg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc gtcggtatgc
     7921 cgcgcactta ctggctgaag gcggaattgg atttctgatt tccctatatc cggaaaatgc
     7981 cgtccgaaca ggtttcagac ggcatttctt ttaaagagat atggcgggat acgcttaaat
     8041 aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt
     8101 cggttgcggc agcattgctc ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag
     8161 ccgaagaaag cttgacgcag cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg
     8221 aatcgcacca atatgagcgc ggctggttta cctctacgga aacgacggtc atccgcttga
     8281 aaccggagtt gctgaataat gcccggaaat acctgccgga caacctgaaa acagtgttgg
     8341 aacagccggt tacgctggta aaccatatca cgcacggccc tttcgccggc ggattcggca
     8401 cgcaggcgta cattgaaacc gagttcaaat acgcgcctga aacggaaaaa gttttggaac
     8461 gcttttttgg gaaacaagcc ccggtttccc ttgccaatac cgtttatttc aacggcagcg
     8521 gcaagatgga agtcagtgtt tccgccttcg attatgaaga actgtcgggc atcaggctgc
     8581 attgggaagg tctgacgggg aaaacggttt accaaaaagg tttcaaaagc taccgcaaca
     8641 gctatgatgc ccccttgttt aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa
     8701 aagtgcattt cgattcggaa acttcagacg gcatcaatcc gcttgctttg ggcagcagca
     8761 atctgacctt ggaaaaattc tccctagaat ggaaagaggg tgtcgattac aacgtcaagt
     8821 taaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc aatcccaacg
     8881 gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc aagaccgggg
     8941 aatcaggcgc gtttatcaac agtgaagggc agttccgttt tggcacactg gtttacggcg
     9001 atgaaaaata cggcccgctg gatattcata tcgctgccga acacctcgat gcttctgcct
     9061 taaccgtatt gaaacgcaag tttgcacaaa tttctgccaa aaaaatgact gaggaacaaa
     9121 tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc tggactgttc accaacaatc
     9181 ccgtattgga cattaaaact tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg
     9241 gaaaaatcat gtttaaagat ttgaagaagg aagatttgaa ccaattgggt ttgatgctga
     9301 agaaaaccga ggtagacatc agaatgagta ttcctcaaaa aatgttggaa gacttggcgg
     9361 tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa
     9421 gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt cagagtatgg
     9481 caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt tctctgaaaa
     9541 acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagccg gattttgatg
     9601 aaggcggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag
     9661 gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat aagccgtgtc
     9721 ggattc
//
LOCUS       DAWFKN010000073         9316 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFKN010000073 DAWFKN010000000
VERSION     DAWFKN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.073"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            90..299
                     /locus_tag="WGR46_001697"
     CDS             90..299
                     /locus_tag="WGR46_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0856590.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            388..1602
                     /locus_tag="WGR46_001698"
     CDS             388..1602
                     /locus_tag="WGR46_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0856591.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLVD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="WGR46_001699"
     CDS             complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="WGR46_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0856592.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALIQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4529..5539
                     /gene="trpS"
                     /locus_tag="WGR46_001700"
     CDS             4529..5539
                     /gene="trpS"
                     /locus_tag="WGR46_001700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0856593.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5545..5886
                     /locus_tag="WGR46_001701"
     CDS             5545..5886
                     /locus_tag="WGR46_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856594.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHTNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6207..6572
                     /locus_tag="WGR46_001702"
     CDS             6207..6572
                     /locus_tag="WGR46_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856595.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(6781..8289)
                     /gene="ppx"
                     /locus_tag="WGR46_001703"
     CDS             complement(6781..8289)
                     /gene="ppx"
                     /locus_tag="WGR46_001703"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0856596.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8405..9137)
                     /locus_tag="WGR46_001704"
                     /pseudo
     CDS             complement(8405..9137)
                     /locus_tag="WGR46_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         2165 a         2414 c         2452 g         2285 t
ORIGIN      
        1 cgatttgaat ttttcagaaa atgaagcgga cgtttgggcg gaggcaactt gtttgataag
       61 atagcaatat ttcaaaacgg agaaagatca tgccttatgt caatattaaa gtaaccggcg
      121 gcaaggaagc accgactgcc gcgcaaaaag cggaactgat cggcggcgta accgaattgc
      181 tggcacgcgt gctgggcaaa aatcccgaaa caacggttgt cgtgattgat gaagtggata
      241 ccgataactg gggaataggc ggcaaaagcg tcagcgaacg gcgcaaagag ggcaggtaaa
      301 aagcctgaaa atctcggttt gatgctttaa attccgcgtg aaaaagagta cattcccacc
      361 cattgcccaa aatttacgga acacattatg gataaatttc ccaagtctgc aaagctcgat
      421 cacgtctgtt acgacatacg cgggccggtt cacaaaaaag cccttcagtt ggaagaggag
      481 ggcaataaaa tccttaaact caatatcggc aaccctgcgc cgttcggctt tgaagcccct
      541 gatgaaatct tggtcgatgt catccgcaac ctgccgactt cgcaaggcta ttgcgattct
      601 aaagggctgt attccgcccg caaagccatt gttcactact atcagaccaa aggtttgcgc
      661 gatattacgg ttgatgatgt ctatatcggc aacggcgtgt ccgagctgat tacgatgtct
      721 atgcaggcat tgctcaacga cggcgacgaa atcctgattc ccgcgcccga ctaccctttg
      781 tggacggcgg cggcaacgct tgcgggcggt acggtacgcc attatctgtg cgacgaagaa
      841 aacggctggt tccccaacct tgtcgatatg gaagccaaaa tcacgcccaa aaccaaagcc
      901 atcgtcgtca tcaatcccaa taatccgaca ggcgcggtgt acagcaggga aatcctgttg
      961 gaaatcgccg aactggcgcg caagcacggt ttgattattt tcgccgacga gatttacgat
     1021 aaaatccttt atgacggcgc ggttcaccac catattgccg cgcttgcccc cgaccttttg
     1081 acggtaacgt tcaacggttt gtccaaagcc taccgcgtcg ccggcttccg ccaaggctgg
     1141 atggtgctca acgggccgaa acatcacgca aaaggttaca tcgagggttt ggatatgctc
     1201 tcgtctatgc gcctgtgtgc caatacgccg atgcagcacg cgattcagac ggcattgggc
     1261 ggttatcaga gcattaacga attcattttg cccggcgggc ggcttttgga acagcgcaac
     1321 agggcgtggg aactggtcaa ccagattcct ggcgtgtcct gcgtcaaacc gatgggtgcg
     1381 atgtatatgt tcccgaaaat cgataccgaa atgtaccgca tccgcgatga catgaaattc
     1441 gtttacgatt tgctggtgcg cgaaaaagtc ttgctggtac agggaacggg gtttaattgg
     1501 attaaacctg accatttccg cattgtcacg ctgccttacg tccatcagat tgaagaggcg
     1561 atgggcaggt tggcaagatt cctgcaaacc taccgccaat aaggggacgg tttgtctgcc
     1621 gaggataaaa aatgccgtct gaaacggaga ttcccgtttc agacggcatt ttcaacagca
     1681 ggaacgaatc aggcaaattt cagtctgtcg ccgtcggctt ctaccctgat ttcgctttcg
     1741 ggcgcatagt ttccggcaag cagggctttt gccagcgggt tttcgatttc cgactggatg
     1801 gcgcgtttga gcggacgtgc gccgtaaatc gggtcgaaac cggctttggc gatgatgtcc
     1861 agtgcggcat cggaaacagt caggcgcagg ttttgttttt ccaaacgttt ttccaaacct
     1921 ttgagctgga ttttcgcaat gctgcggatg tttgcctgat ccaaaccgtg gaacacgacc
     1981 acttcgtcga tgcggttgat catttcagga cggaaatgtt ctttcacatc ctccatcaca
     2041 acttctttta ccgcttcgta atcctgaatg cccatttgtt ggatgtgctg gctgccgatg
     2101 ttggaagtca tcacaatcac ggtattttta aaatctaccg tcctgccttg cccgtcggtc
     2161 aaacgaccgt catccaatac ttgcagcagg atgttgaaca catcgggatg ggctttttcc
     2221 acttcgtcca gcagaatcac gctgtacggt ttgcggcgca cttgttcggt caggtagccg
     2281 ccttcttcgt agccgacata acccggaggc gcgccgatta agcgggcaac ggcgtgtttt
     2341 tccatatatt cggacatatc gatgcgaatc agatgatctt cgctgtcgaa cagaaagcct
     2401 gccagggctt tacacaactc ggttttaccc acgccggtcg ggcccaagaa caggaagctg
     2461 ccgtaaggct tgttcggatc ggcaagaccg gagcggctgc ggcggatagc gtcggacacg
     2521 gcacgcacgg cttcgtcctg tccgaccacg cggcggtgca atacttcttc cattttcagc
     2581 agtttgtcgc gttcgccttc catcattttg gatacgggaa tgccggtcat acgggaaacc
     2641 acctctgcga tttcctctgc gccgacatta ttccgcaaga gtttgtttgc cggttttgtg
     2701 ctgtccgtat ctgcccgttc ggcggctgca cgctgttttt ccaaatgctc caaatcttca
     2761 tacatcaatt ttgaagccag tgccaaatcg ccttgccgtt ttgcctgttc gattttaatt
     2821 ttgacttcgt caatttgttt cttaatatta gcagcaccgt ctgaaattgc tttttcggct
     2881 ttccagattt cgtctaaatc ggcgtattct ttttgcagac cgttgatttc ctcgtctatc
     2941 agttccaaac gttttttgct ggcatcgtct ttttcttttt caacgtgcgc cttttccatc
     3001 cgaagctgaa ttagacggcg gtcgattttg tccattgctt ccggcttggt ttctttttcc
     3061 atcttgacac ggctggcggc ttcgtcaatc aaatcaatcg ctttatcagg caggaagcgg
     3121 tcggtgatgt agcggtcgct caattccgca gcggcaacga tggcggggtc ggtgatgtcg
     3181 atgccgtggt ggatttcata acgctcctgc aaaccgcgca ggatggcgat ggtgtcttcc
     3241 acactgggtt cgccgaccaa tactttttgg aaacggcgtt cgagtgccgc atctttttcg
     3301 atgtattggc ggtattcgtc caaggtggtc gcgccgatgc agtgcaattc gccgcgtgcc
     3361 aaagccggtt tcaacatatt gcccgcgtcc attgcgccgt cagttttgcc tgcgccgacc
     3421 aaagtatgga tttcatcgat gaaaatcagg gtgttgccgt cgtctttcgc caaatcgttc
     3481 aatacgcctt tcaagcgttc ttcaaattcg ccgcggtatt tcgcgccggc aatcaaagcc
     3541 gccaaatcca aaaccagcag gcgtttgtta cgcagggatt caggtacttc gccgttgacg
     3601 atacgttgcg ccaagccttc aacaatagcg gttttaccca cacccggctc accaatcagc
     3661 acagggttgt ttttggtacg gcgttgcaat acctgaatcg cgcggcggat ttcgtcgtcg
     3721 cggccgataa cggggtcaag tttgccgtcg cgggcgcgtt gagtcaggtc aagtgtgtat
     3781 tttttcaaag catcgcgttg gtcttcggca ttcgcatcat tcacgttttg tcctcctcgt
     3841 accgcgtcaa tcgcggcatt gatattttgt tcggtcgcgc cggcttcttt caaaattttg
     3901 ccggtcgcat cgttctgctg aatcaaggca agcaggaaaa gttcgctggc gatataggca
     3961 tcgccacgtt tggttgctgc tttgtccatc aggttcaaca ccgcctgcaa ttcgcggctg
     4021 ggcagaatat cgccgccctg accggacact ttcggcaggc tgctcaaatg ctgctgcaaa
     4081 cgctgtttca cctgcggcac gttcacgccc gaatgcgcca gcaacgcggc ggctccgctg
     4141 ttttggtcgt caagcagggc ttttaacaca aagcctgctt ctagatagct gccgtccgca
     4201 gccaacgcca aactctgagc ttctgcaagg gcttgttgga atttggcggt taatttgtcg
     4261 tatcgcattt ttgtttcctt ttcaaaatgt ccgctgtcga agcctatatg tgcataattg
     4321 tggataactc aagttctgtt ttctgttttt ctatatttaa ttcgatatat cattgaattt
     4381 aaagtatata aaaatgtata ataatgtgta taactatatc ttcttaatat ggaaaagtct
     4441 gttgtcggct ggatgtaggt ggcaaatcgg gtataatcgg cgcatctttt tccctttcag
     4501 acggcattga tgccgcaagg acatttttat gagcaaaaaa cgagttctga ccggcgtaac
     4561 caccaccggc atcccgcatc tgggcaacta cgtcggtgct atccgccccg ccgtccgcgc
     4621 ggcgcaaaac cctgataccg aatccttcct cttcctcgcc gattaccacg gtatcatcaa
     4681 atgccacgag ccggagatga ttcaccaatc cacccaagcc gttgccgcca cttggcttgc
     4741 ctgcggactc gaccccgagc gcaccacctt ctaccgccaa agcgacattc ccgaagtgat
     4801 ggaattgaac tggattctga cctgcatcac cgccaagggt ttgatgaacc gcgcccacgc
     4861 ctacaaagcc gccgtgcagg caaatgcgga aaacgggcag gaagaccccg atttcggcgt
     4921 ggaaatgggt ttgttcagtt atccgattct gatgactgcc gatattctga tgttcaacgc
     4981 caacgaagtc cccgtcgggc gcgaccaaat ccaacacgtc gaaatggcgc gcgacatcgc
     5041 cggccgcttc aatcaccgct tccgagaact cttcaccctg cccgaagtga aaatcgacga
     5101 aaacgtcgaa ctcttggtcg gtttggacgg acgcaaaatg tccaaatcct acggcaacac
     5161 cattccgctt tgggaaaacg acaaaaaaac ccaaaaatcg gtcaacaaaa tcatcaccaa
     5221 tatgaaagag ccgggcgagc cgaaacagcc cgacgaaagc ccattgtttg aaatctacaa
     5281 agccttctcc acgccgtctg aaacggtgga atttacgaaa atgcttgccg acggcctggc
     5341 ttggggcgag gcaaaaaaac ttttggcggc gaaaatcaac gccgaactcg ccgaaccgcg
     5401 cgaacgctac aacgagctga ccgccgaccc ttcgcaaatc gaagagattt tacaggcagg
     5461 tgcgcaaaaa gcgcgtaaag aagcacgcga attgttggac aaagtacgcg atgcggtcgg
     5521 catccgcccg ttgaaataaa cccgatgccg tccgaacctc ttaaaggctt gccggaacaa
     5581 aacgtcgtcc ggctgatcgg caagcatacc aacgacttgg aagccgtcgt cggcaaatgt
     5641 atggaaaccg acggaaaggg cgcgccttcg ggctgggcgg caaacggcgt gtgccatacc
     5701 ttgtttgcca aactggtagg caatatcgcc gaagacggcg gcaaactgac ggattacctg
     5761 atttcgcatt ccgccctgca accctatcag gcaggcaaaa gcggctatgc cgcagtgcag
     5821 aacggacgct atgtgctgga aatcgacagc gagggggcgt tttatttccg ccgccgccat
     5881 tattgaggta ttcggacatc ccggaatata tttgggtttt tcaaaccctg caggaaaaag
     5941 tccgcaccga cgagaaacgc aaaaggaggg ggatatgtgt tacaattttc cgaactgttt
     6001 tcataaaata gttttcggac gtgtttcaat atggcattga tgccgcctta ttgttcgaga
     6061 aaaaaaacct ttatatttaa atataatggg ttttaactaa acgggaaacc gttttctctc
     6121 cggccgatgg gcaaaatcag ccgattgatg gaacacggtc cgatttttaa gcaaaacctt
     6181 aatcaacaac tcaagaagga taagacatga aaaaattatt gattgccgca atgatggcgg
     6241 ctgccttggc agcttgttcg caagaagcca aacaggaggt taaggaagcg gttcaagccg
     6301 ttgagtccga tgttaaagac actgcggctt ctgccgccga gtctgccgct tctgccgtcg
     6361 aagaagcgaa agaccaagtc aaagatgctg cggctgatgc aaaggcaagt gccgaggaag
     6421 ctgtaactga agccaaagaa gctgtaactg aagccaaaga agctgtaact gaagccaaag
     6481 aagctgtaac tgaagcagct aaagatactt tgaacaaagc tgccgacgcg actcaggaag
     6541 cggcagacaa aatgaaagac gctgccaaat aatttgttgc tttggcaaaa atgacgagat
     6601 gccgcctgcc ggcaacccaa acgaaaccgc ctgaagattt tcaggcggtt tttgagttgt
     6661 atgccccttg ttttttacgc ctgcataacc gttccgagca gatgtgccgc gcatatcggg
     6721 atgttgcgga cagggaaatg ccgtctgaac atcattcaga cggcatttgc cccgcaaggt
     6781 tcagacggcc tcgactttga acggcatatt gattttttgc cattggacgc tttcatattc
     6841 caatgcgtcg gcaatcaggg gatggcgttc cagccattcc ctgtcaatac gcaggatgaa
     6901 gccgcaactt tccgtgtccg tacgcaactg catatttttc gggaaagaca ggtcttggcg
     6961 cgaacggcag aacagggcgg caaggcgcag cgacaaaacg gcataccaca acatttcgtt
     7021 ggtgccgata atgccgccca tttttttcat atcgccgcga tgaccgatga ccagttgggc
     7081 aagtatggtc tgttctttgc gtgagaaacc cggcatatcg gcgttttcga ggatgtaggc
     7141 ggaatgcttg tgatagccgg tgtgggcgat gtccaaaccg atttcgtgca gcgcggcggc
     7201 gcgtccgaga tactgttgcc acaaggcaag ctcttgaacc gtaacatttt tagcgtggca
     7261 gaggctgtcc ataaaggttt gcgcggtctc ggcggtgcgt ttcgcctgat tgaggctgac
     7321 gtggtagcgg tgttggaact cggcaaccgt ttgtccgcgc atatcttcgt ttaaaccgcg
     7381 cccgatcaaa tcgtaaaaca cgccgtcgcg cagggcggct tcggttacgg tcatcctgtc
     7441 gagtttcatt tcttcaaacg ccgccatcat cacggcaagt ccgccggcaa aaacttcgat
     7501 gcgttccggt ttcaggtttt caaatttggc ctttttgacc gaaccggctt cgatgatgcg
     7561 ttcggcgagg gcgcgcatgc ctttgtaggt aatgtccgcc tcttggggca tttcggcggc
     7621 aagcacgtcg cggatggatt ttgccgaacc cgatgtgccg acggcgaaat cccaaccttc
     7681 gcgcctcata tttttgctga tgcgctggat ttcgttgcgg gcggcggaaa tggcagattg
     7741 gaagtctttg gcggtgattt tgttttggaa gaagcgcagg ctgtaggtaa cgcagcccaa
     7801 gggcaggctt tcggtaatgt cgggattcag cgtcgagccg atgacaaatt ctgtcgaacc
     7861 gcccccgatg tcgataacca gcattttgcc gccgccgggg gggagggtgt ggatcacgcc
     7921 ggtataaatc agccgtgcct cttcgcgccc ggcgatgatt tcgatgggga aacccagtgc
     7981 cgcttcggct ttgggaagga aatctgcgat gtttttggcg acacggaatg tattggttgc
     8041 cacggcgcgt acctgttcag ggcggaagcc gcgcaggcgt tcgccgaatt ttgccagaca
     8101 gtccaaagcc tgttcttggg aagcggcact caggtttttc tgttcgtcca gtccggcggc
     8161 gaagcgcacc atctgtttga acgaatcgat gactttcaac tgtccgttgt tgttttcgca
     8221 aatctggagg cggaaactgt tagaacccaa atcgacggag gcaaggacgt ttgcgggggt
     8281 ggtggtcatg gcggataccg gtgggggaaa aacgcaatgt tactctgacg gcgcaggcgt
     8341 tgacaataaa tgatgcggcg gtttttgatt ctgcccacgg atgttgccga cggcattttt
     8401 gcgcttattt gaaatccttt tccacgctca tgaaaatctg cgtgtttttg cgtgtgtaaa
     8461 aacttttcat attgctgtcg attttcagat agcggaaatt gagttgcggc gtaaagccct
     8521 tccaagagat tttgtcgtgc cacaacgaca ggtttgcctg atattcgtgg tctttgcgcg
     8581 ggaagcggta cacaatggtc ccgggtgcgt caaacatcct gcgggtatag cgcaggtttg
     8641 cccgcagacc caagcccgcc gtcgaacgtt ttgactgcgc cgacacgcaa acccttgcgg
     8701 acggaagcct gttccgcctc tttcgtcatg ttgtgcgacc agtccgcacc gccgtaaagc
     8761 agccagtctt tcggcgcgga atacatcagc gtcgcgcccg ccagcggcat atggctgtcg
     8821 tatcgggcgg cggtgcggtc ttcctgataa cgcttccaca tattgcccgc gtttagtgtc
     8881 aaccgccagc gttcgcccaa gcgttgggag aaatcggcat tgaagccgcc gacgaaattg
     8941 tatcggctgc cgcctaagag gttttgcccg acaaacggca cgatgccgaa cgagcgcgtt
     9001 accgaacgat ttttatagcc gagcgacagg tgcaggcact gttcgctgaa atctttgtta
     9061 tcccaataat gcacgccgcc gccgctgatg ccgccgtaga ggaaatgatg cccgcccgca
     9121 ttgatttcgc gcgacacgcc taagccctag cgcaaaccgt gtgccttttg cggcaggctg
     9181 tcggcggttt tcgtccagct tctgcccgca aattcaatcg tttttttgga cgaagcgttg
     9241 tttatagcgg attaacaaaa atcaggacaa ggcgacgaag cctcagacag tacagatagt
     9301 acggaaccga ttcact
//
LOCUS       DAWFKN010000074         9208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFKN010000074 DAWFKN010000000
VERSION     DAWFKN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.074"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(232..1155)
                     /locus_tag="WGR46_001705"
     CDS             complement(232..1155)
                     /locus_tag="WGR46_001705"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0856597.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
     gene            complement(2179..2501)
                     /locus_tag="WGR46_001706"
                     /pseudo
     CDS             complement(2179..2501)
                     /locus_tag="WGR46_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(2488..2835)
                     /locus_tag="WGR46_001707"
     CDS             complement(2488..2835)
                     /locus_tag="WGR46_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0856598.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQKVEVAKGVITAQMASSNVNNE
                     IKGKKLSLWAKRQNGSVKWFCGQPVQRDKADADADADAVTADTAAANKKIDTNHSHEP
                     TSRHSRESGNDGI"
     gene            complement(2896..3198)
                     /locus_tag="WGR46_001708"
                     /pseudo
     CDS             complement(2896..3198)
                     /locus_tag="WGR46_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(3227..3556)
                     /locus_tag="WGR46_001709"
     CDS             complement(3227..3556)
                     /locus_tag="WGR46_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0856599.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(3634..3852)
                     /locus_tag="WGR46_001710"
     CDS             complement(3634..3852)
                     /locus_tag="WGR46_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0856600.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            3974..4927
                     /locus_tag="WGR46_001711"
     CDS             3974..4927
                     /locus_tag="WGR46_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0856601.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYPRDVAAGLWEKSPFFCYYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(5002..7059)
                     /gene="metG"
                     /locus_tag="WGR46_001712"
     CDS             complement(5002..7059)
                     /gene="metG"
                     /locus_tag="WGR46_001712"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0856602.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(7175..9013)
                     /gene="glmS"
                     /locus_tag="WGR46_001713"
     CDS             complement(7175..9013)
                     /gene="glmS"
                     /locus_tag="WGR46_001713"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0856603.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1956 a         2202 c         2628 g         2422 t
ORIGIN      
        1 agattgcggc atttatcgga aaaaacagaa accgctctgc cgtcattccc gcgcaggcgg
       61 gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtcc gagattctag
      121 attcccactt tcgtgggaat gacgaatttt aggtttctgt tttggttttt tgtccttgta
      181 ggaatgatga aattttaagt tttaggaatt taccggaaaa aacagaaagc gttatccaca
      241 atttctgatg ttcagctcgt gaaatgcgtc gggcaaatca tcgctgtcgg caaattccac
      301 ccggtcgtaa gccgtttcgt ctgccaaaac cgcgcgcaag agtgcgttgt tgatggcgtg
      361 tcccgatttg tagccttcaa acgcgccgac aatcggatgt ccgacgatat acaaatcgcc
      421 gatggcatca aggattttgt ggcgcacaaa ctcatcggga tagcgcaagc cttcaggatt
      481 caggacatcc gtgtcgtcaa tcacgatggc gttgttcaaa ttgccgccca aacccagatt
      541 gtgggcgcgc atcatttcca cttcgtgcat aaagccgaag gtgcgcgcgc gcgcgatttc
      601 gtcgatgtag gatttgccgg cgaaatcaat ttcaaaagtg ggcgagctgc ggttgaaaac
      661 cggatggtcg aattcgatgg tcagcgttac cttaaagccg tcatacggcg taaagcgtac
      721 ccatttgccc gcttctttga tttcgacagg cttgaggatt ttcaaaaaac gcttttgcgc
      781 cttttgatcg accacgcccg catcttgcaa aaggtaaata aacggcaggc tggagccgtc
      841 cataatcggg atttcgggcg cgttcagctc aatcagcgca ttgtcgatgc cgtaggcgga
      901 cagcgcggac ataatgtgtt cgattgtgcc gacgcgcacg cctttgtcgg taacgatggt
      961 ggaggaaagg cgggtatcgt tgatcaaata aggggtcagt ttgatttgtt cgcccatctc
     1021 gccgtccaaa tcggtacggc ggaaggaaat cccgctgttt tcaggcgcgg ggtgcagggt
     1081 cagcgcgacg cgttcgcccg aatgcagccc gactccggta acgctgatgg atttcgccaa
     1141 agttctttgc agcataaacc gcttccttat caatggggta agttttggaa taatacgata
     1201 aaaccggaaa aacaagctat gtttttccat agtatttgcc aatgtatctg ttttcaatac
     1261 gtaagccgca taaaaatgta tagtggatta acaaaaatca agacaaggcg acgaagccgc
     1321 ccctctcgaa aaacgcaaaa aatgccgtcc gaaaaccttt cggacagcat tttcgcgtga
     1381 accgtcattc ccacaaggac aaaaaaccaa aacataaaac caaaaacaga aacctaaaat
     1441 tcatcattcc cacgaaagtg ggaatctagt tcgttgagtt tcagttattt agaataaatt
     1501 ttgaaactta aatcccgtca ttcccacgaa cctacattcc gtcattccca cgaaagtggg
     1561 aatccagttt tttgagtttc agtcattccc gataaattgc cttagcattg aatgtctaga
     1621 ttcccgcttt tgcgggaatg acggcggcgg gttgctgttt tttgtttata aatttctgta
     1681 gctttgaatt tcagccatct tcaataaatt atagcggatt aacaaaaacc agtacggcgt
     1741 tgtctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg
     1801 ttccgtacta tttgtactgt ctgcggcttc gccgccttgt cctgattttt gttaatccac
     1861 tatatctagc cgaattactt tattttttga tacgcaaccg gccggttgcc gtcattcccg
     1921 cgcaggcggg aatccagtcc gcttgttcgg tacatgagag ggcggattgc gcgcctgtca
     1981 gataaacccc gtgtttaaac gggcggcaat gaggtacgcg cagagccttg aagcgcaatc
     2041 gatatattat tttcagccaa aacggacgcc cccgcttgcc ttgcaaaccc ttaaaaagga
     2101 agccacccgg attaatccga gtggccgtgg aaaatcactt accgcttgat ttatttaaaa
     2161 tttatggtat aatttgcctt atccggtaga tgaatcatcg cggcaggttg acggcaggtg
     2221 cttggtgttg attttgtcgt cggtgccgtt ggcggtggcg gctttgacgt cgtctttggc
     2281 tttgtcggcg cgcgcaaccg gctgtccgca gaaccatttt accgaaccgt tttgacgctt
     2341 ggcccacagg gagagttttt tgcctttgat ttcgttgttt acgccgcttg aaagcattgt
     2401 ggcggtaatg acgccgtttg cgactttaac ttccttaaca tatttgcctt tgatgtcggt
     2461 gggggggatg ccacgccggc ggcatcatta aatcccgtca ttcccacttt cgcgggaatg
     2521 acgggatgta ggttcgtggg aatgattggt gtcgatcttc ttgttggcgg cggcggtgtc
     2581 ggcggtaacg gcgtcggcgt cggcgtcggc gtcggctttg tcgcgctgaa ccggctgtcc
     2641 gcagaaccat tttaccgaac cgttttgacg cttggcccac agggagagtt ttttgccttt
     2701 gatttcattg tttacgttgc ttgaagccat ttgggcggta atgacgcctt ttgcgacttc
     2761 aactttctga acatatttgc ctttgatgtc ggagggggag gatgccacgc cggcggcatc
     2821 attaaatccc gtcattccca ctttcgtggg aatgacggga ttaaagtttc aaaatttatt
     2881 ctaaataact gaaactcaac gaactagatt cccgcttttg cgggaatgac gaagtttttc
     2941 tgccatttgc cgtgattcgg gcaatactcg gtaacggctg attttttgaa agtgtttgaa
     3001 atcggcgcgt ggtgtttttg tgcaaacggc agatgacgca tcgcggcagg ttgacggcag
     3061 gtgcttggtg ttgatgttgt cggcggcggt gcgcgtaacc ggctgtccgc agaaccattt
     3121 taccgaaccg tcttgacgct tggcccacag ggagagtttt ttgtctttga tttcgttgtt
     3181 tacgttgctt gaagccatta tgtcagacgg tattgcccgg gcagctttat tcgtacactt
     3241 tcagcagctc gacttcaaat atcaaagtgg cgtgcggggg aatcacgccg cccgcgccgt
     3301 gtgcgccgta gcccatttcc gaagggatgg tcagcttgcg tttgccgcct tccttcattc
     3361 cgccgaagcc ttcgtcccag cctttgatga cttgtccgac accgagcgtg atggtcagcg
     3421 gctggcggcg gtcgaggctg gagtcgaatt tggttccgtt ttccagccaa cctgtgtaat
     3481 gcacggtaat ctctttgcct ttaactgctt cttttccgaa gccttcttgc aagtcttcaa
     3541 taatcaggcc gcccatattt gtcctttcat tgcttgttgg tcaaaacggc aagggtaaca
     3601 taccgtccgt cgaagtcaaa tgccgctcaa acgtcagctg catcggtgca gctgaaacgg
     3661 ctgtgtttgt tggactgttt tatttttttc gtaaaggttc catgcttttt catggaaata
     3721 gaaaacgacg gtgttgatta ggggctcgac cagcgcgacc gcccccgata cgcctatact
     3781 gcccgtcagt acataggtta cactgaaggc ggtgctgaaa tgcagtgcgg caaaagtcag
     3841 ggttttaagc atcatcctct cccgaattgg acattgacgg aggaatgata aaggttatca
     3901 taaggttgcg cggtttaaat ttgctatttg ttgttagtgt aaataaatca tttttcaaat
     3961 ggggatagaa attatgaatc ataaaaagat cgttgttttg gatgcggata ctttgcccgg
     4021 ccgggttttt cattttgatt ttccgcacga gcttgcggtt tacggtacga caggtgcgga
     4081 tgaaacggca gaacgggtgc gcgatgcaca tattgtcatt actaacaaag tgatgatttc
     4141 tgccgatatt attgcggcta atccgcagtt ggagctgatt gccgtcagtg cgaccggtgt
     4201 gaacaatgtc gatattgggg cggcgaaggc ggcgggcgtt gcggtatgca atgtccgcgc
     4261 ctacggaaac gaatcggttg cggaacacgc ctttatgctg atgattgcgt tgatgcggaa
     4321 tttgcccgcc tatccgcgtg atgttgcggc aggattatgg gagaagtcgc cgtttttctg
     4381 ctattacggt gcgccgattc gggatttgaa cggtaaaacg ctggcggttt tcggacgcgg
     4441 caatatcgga cggacgcttg ccggatacgc gcaggcgttc ggtatggggg tggtgtttgc
     4501 cgaacacaaa cacgcgcccg acgtgcgcga aggctatgtt tcctttgaag atgcggtaca
     4561 gactgccgac gtgttgtcgc tgcactgtcc gctgaacgcc caaactgaaa atatgatagg
     4621 cgaaaacgaa ttacagcaga tgaagcccgg tgcggttttg attaattgcg ggcgcggcgg
     4681 gctggtggat gaaaacgcgc tgcttgccgc actcaaatac gggcagatcg gcggggcggg
     4741 tgtcgatgtt ttgacgaatg agccgcccaa aaacggcaat cccttgctga atgcacgatt
     4801 acccaatctg attgttacgc cgcataccgc gtgggcaagt cgtgaggctt tggacaggct
     4861 gtttgatata ttgttggcga acattcacgc ctttgtgaaa ggagaggcgc aaaaccgcgt
     4921 ggtttgagct tgtcgggatg acggaaaaca atgccgtctg aacgcccaaa gggttcagac
     4981 ggcatttttt gagattcctg tttaaccgac tttgtcgccc ggctgcgcgc ctgtatccac
     5041 atctaagagc ttcagtttcc cgtctgccgt ggcggcactc aaaatcatgc cttcggatac
     5101 gccgaatttt gccattttgc gcggggcgaa gttggcgacg gcgatgacca ttcttccgtt
     5161 taactcggca gggttggggt aaaacgcggc gatgccggag aagatgatac gtttttcaaa
     5221 accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct tcgacggctt cgcagttcaa
     5281 tactttggcg acgcgcatat cgattttcat gaagtcgtcg aaacttgcct gttcggcgac
     5341 tttttcgtat ttgccctctt cggcggcagg tgcggctgcg gcggcgatgc tttgtttgtt
     5401 ggcttcgatt aaatcgtcca cttgtttttg ctccactcgt tgcattaaat gtttgtattt
     5461 gttgatggcg tgtttgccca aggtttcgcg tgtatttgcc caagtgatgg cttccaaatt
     5521 caggaatttg gcggcgtttg cggcggtttg cggcaagacg ggggcgaggt aggcggtcaa
     5581 catggtgaag gcgttgatga gctcgctgca cacttcgtgc aggcgttcgt cttgaccttc
     5641 ctgtttcgcc agttcccaag gtttgttggc atcaacgtat tcgttcacgg catctgccaa
     5701 tgccatgata tcgcgcaggg cgcgggcgta ttcgcggttt tcgtattgct cggcgatggt
     5761 gtcgctttcg gcggcaagtt ttgccagcaa tgcgctgtcg gcaacgtctt tcagacggcc
     5821 ttcaaagcgt ttggcgataa aacctgaggc gcgggcggcg atgttgacgt atttgccgac
     5881 gaggtcgctg tttacgcgcg aaataaagtc ttgcaggttc aaatcgatgt cttcgatttt
     5941 gctgttgagt tttgcggcga tgtagtagcg catccactcg gggtttaaac cttgttccag
     6001 ataggatttg gcggtgataa acgtgccgcg cgatttggac attttttgtc cgtctacggt
     6061 caaaaagccg tgtgcgtaca cgccggtcgg ggcgcggtgg ccggagaaat gcagcatggc
     6121 aggccagaac agggcgtgga aatagaggat gtctttgccg atgaagtggt acatctcggt
     6181 ttggctgtcg gctttgaagt attcgtcaaa atcgacgccg atgcggtcgc acaggttttt
     6241 aaacgacgcc atgtagccga cgggcgcgtc cagccagacg tagaagtatt tgcccggcgc
     6301 gtcggggatt tcaaaaccga aatacggcgc gtcgcgggaa atatcccagt cggacagggt
     6361 ggtttcttca ccttcgccca gccattcttt cattttgttg agggcttcgg cttgcagatg
     6421 gggcttgccg tcgtgcgggt tgttgccgga agtccatgct ttgaggaagt cggcgcattc
     6481 tcccagtttg aagaagaagt gttcggattc gcgcaattcg ggtttcgcgc cggaaacggc
     6541 ggaatacggg ttaatcagtt cggtcgggga ataggtcgtg ccgcagactt cgcagttgtc
     6601 gccgtattgg tcttgggcgt ggcatttcgg gcattcgcct ttaacgaagc ggtcgggcag
     6661 gaacatttgt ttttcggggt cgaaaagctg ctcgatgacg cggctttcga ttttgccgtt
     6721 ggctttcagt gcgcggtaaa tgtcttggga aaactgtttg ttttcagggg aatgggtgct
     6781 gtaataattg tcgtagccga tgaaaaaacc ggtaaagtcg gcgaggtgtt cttcgcgcac
     6841 tttggcaatc atgtcttcgg gcgcaatgcc ttgtttttgc gcggcaagca tcacgggcgt
     6901 gccgtgggtg tcgtcggcgc agcagtagtg gcactcgtgg ccgcgcagtt tttgaaagcg
     6961 cacccaaacg tcggtttgga tgtgttcgac catgtggccg aggtggatgc tgccgttggc
     7021 atagggcagg gcggaggtaa cgaggatttt gcgtgtcata ttgtgctttg caaacaatgg
     7081 gtaaaggcgg attataccgc aaatcaaacg gggaaatgcc gtctgaagcc tgaaaaatcg
     7141 ggcttcagac ggcatttttg ccaaccggcg ggatttattc gacggttacg gatttcgcca
     7201 gattgcgcgg cttgtctaca tccgtgccgc gcgcgagggc ggtgtggtag gaaaggagct
     7261 gcacggggat ggtatgcacg acgggggaca gttcgccgac atggcgcggc gcacggataa
     7321 cgtgcacgcc ttcggttgca ttaaaattgc tgtcgaggtc ggcaaagacg aaaagttcgc
     7381 cgccgcgtgc gccgacttcc tgcatattgg ctttgacttt gtccaacagg ctgtcgttgg
     7441 gcgcgatgac gacgacgggc atgttttcgt ccaccaaggc aagcggcccg tgcttcagtt
     7501 cgccggcagg ataggcttcg gcgtggatgt aggtgatttc cttcagcttt aacgcgcctt
     7561 cgagggcaat cgggtaatgg atgccgcgcc ctaaaaacag cgcgctggtt ttcttggcaa
     7621 actgttgcgc ccaggcggca atttgaggtt cgaggtttaa agcgtgctgc acgctgccgg
     7681 gaagctggcg gagttcttcg gtgtaacgtg cttcatcttc gccggatacc aaaccgcgca
     7741 ctttcgccag cgttaccgcc aaaccgaaca gcgcgaccag ctgcgtggta aacgccttgg
     7801 tcgaggcgac gccgatttcc gcaccggcac gggtataaag cacgaggctg ctttcgcgcg
     7861 gcagggcgga ttccatcacg ttgcagatgg agaggctgtg gcggtgtccc aaagatttgg
     7921 cgtatttcaa cgcctccatc gtgtccagcg tttcgccgga ttgggaaatg gtaatgacca
     7981 gttggtccgg atcggcaatc acgctgcggt atcggtattc gctggcgatt tcgacgtcgg
     8041 tcggaatttt ggcgatggat tcgagccagt attttgcggt cagcgcggcg taataggacg
     8101 tgccgcaggc aaggattttg acgctgcgga tgctttcaaa cacgcttttg gcgtctttgc
     8161 cgaagttttc gggaatgaag ccgccgtcga ggaaaacctc cgccgtgtct gcaatcgcgc
     8221 gcggctgctc atggatttcc ttttgcataa agtggctgta cggtcccagt tccaaagagg
     8281 cgagcgagag ttcggatacc ttgactttgc gttcggcagg caggccgttt ttatcggtca
     8341 gccttttgat gccgtctgaa gccagcagcg cgatgtcgcc gtcttcgagg tacgccacgc
     8401 ggcgcgtaaa ggcgatgacg gcggatacgt ccgaagcgat aaaggtttca tcgtcgccca
     8461 aagcgaccaa aagcgggcag cccatacgcg ccacaactaa ttcatcaggc ttgtcttggg
     8521 caataaccgc gatggcgtat gcgccgtgga aacgtttgac cgcttcttgt accgcttcaa
     8581 acagcctgcc gccgttttgc gcgtattcgt gattgatgct gtgtgcgatg acttcggtat
     8641 ccgtttgcga ttcaaaacgg tatcccaaac cttccaaacg tttgcgttcg ctttcaaagt
     8701 tttcgatgat gccgttgtgt acgaccgcaa tcataccgcc gctgatgtgc gggtgggcgt
     8761 tcggctcagt aacgccgccg tgtgtcgccc aacgcgtatg tccgatgccg atgccgccgc
     8821 tgatgccttt ttcgcgtgcc gcgtcctcca taagctgcac gcgtccgacg cggcgcacgc
     8881 gtttgatttt gccgtcggtg ttgacggcaa tgcctgatga gtcatacccc cggtattcga
     8941 ggcgtttgag gccgtcggtc agaaaatcga cgacgttgtg atgggcgcgg atggcgccga
     9001 cgataccgca cataactgtt ccttagtatc aggttgaaaa aaaacaggcg cggacggctc
     9061 ccgtgccgca ccttccgctt cggattataa accgcctagt gtcggaaaac agcaaaatgc
     9121 cgtctgaagg cttgggcttg ctcaaaaaag gagggatttc cccgcctgac cgggatgggc
     9181 gttcagacgg cattacctgc cgccggtt
//
LOCUS       DAWFKN010000075         9091 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFKN010000075 DAWFKN010000000
VERSION     DAWFKN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.075"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            218..997
                     /gene="pgeF"
                     /locus_tag="WGR46_001714"
     CDS             218..997
                     /gene="pgeF"
                     /locus_tag="WGR46_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0856604.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1049..1528
                     /locus_tag="WGR46_001715"
     CDS             1049..1528
                     /locus_tag="WGR46_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856605.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1530..2528
                     /locus_tag="WGR46_001716"
     CDS             1530..2528
                     /locus_tag="WGR46_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0856606.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2649..3068
                     /locus_tag="WGR46_001717"
     CDS             2649..3068
                     /locus_tag="WGR46_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856607.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3144..3659
                     /locus_tag="WGR46_001718"
     CDS             3144..3659
                     /locus_tag="WGR46_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0856608.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3743..4627)
                     /locus_tag="WGR46_001719"
     CDS             complement(3743..4627)
                     /locus_tag="WGR46_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0856609.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5111..5974)
                     /gene="rpoH"
                     /locus_tag="WGR46_001720"
     CDS             complement(5111..5974)
                     /gene="rpoH"
                     /locus_tag="WGR46_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0856610.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6238..7788)
                     /gene="lnt"
                     /locus_tag="WGR46_001721"
     CDS             complement(6238..7788)
                     /gene="lnt"
                     /locus_tag="WGR46_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0856611.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALIAALLAAGYT
                     ARQTDFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPET
                     AIPVMRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQD
                     GIPYYAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNI
                     CYEDGFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATN
                     TGATAIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALIL
                     FIFRNKEH"
     gene            complement(7857..8225)
                     /locus_tag="WGR46_001722"
     CDS             complement(7857..8225)
                     /locus_tag="WGR46_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0856612.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8125..8583)
                     /locus_tag="WGR46_001723"
                     /pseudo
     CDS             complement(8125..8583)
                     /locus_tag="WGR46_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8777..9013
                     /locus_tag="WGR46_001724"
                     /pseudo
     CDS             8777..9013
                     /locus_tag="WGR46_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT         2001 a         2250 c         2668 g         2172 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact atataaagct cggagaaggt atataaggac
       61 atcattcctg caccggcaag aatccgaacc cgaacgtttg aaaacaatcc cgaatctccg
      121 aattcccgcc tgtgcgggaa tgacgaaaaa acaagcatcc atttgccccg aaggcagtta
      181 atcaaccctt tccgccacac acctattcca atatccaatg aaaaccatca cagaaaccct
      241 aaatctcgcc ccgaaaggca aaaacttcct gaccgccgat tggcccgcgc ccgccaatgt
      301 gaaaaccctg attaccacgc gcaacggcgg cgtgagccaa ggtgcgtatc agagtttgaa
      361 cctcggtacg cacgtcggcg acaatcccga agccgtgcgc cgcaaccgcg aaatcgtgca
      421 acagcaggtc ggattgcccg ttgcctacct caatcaaatc cacagtaccg tcgtcgtcaa
      481 tgctgccgaa gctttgggag gcacacccga tgcggacgct tccgtagacg acacgggcaa
      541 agttgcctgt gctgtcatga ctgccgactg tctgcccgtt ctattttgcg acagggcggg
      601 tacggcggtt gccgccgcac acgcgggctg gcgcggtttg gcgggcggcg tattgcaaaa
      661 caccattgcc gcgatgaagg ttccacccgt cgaaatgatg gcgtatctcg gtcccgccat
      721 cagtgcggat gcgtttgaag tcggacagga tgtgtttgat gcgttctgca cgcccatgcc
      781 cgaagccgcc gccgcatttg aaggcatagg cggcggcaaa ttccttgccg acctttacgc
      841 gctcgcccgc ctgattctga agcgcgaagg cgtgggcggc gtatatggcg gcacgcattg
      901 tacggttttg gaacgggata ctttcttttc ctaccgccgc gacggagcga cagggcgtat
      961 ggcgagcctg atttggctgg acggcaatgc cgtctgaaca cgccgctgat ataatctacc
     1021 gactttgtgc ttttgagaaa ggcaagccat gaacaaactg tttcttactg ccgcagtgct
     1081 gatgctgggc gcgtgcggtt tccacctgaa aggtgcagac ggcatttctc cgccgctgac
     1141 ctaccggagc tggcacatcg aaggcggaca ggcattgcag tttcctttgg aaaccgcgct
     1201 gtatcaggct tcgggcaggg tggacgatgc tgccggcgcg cagatgaccc tgcgtataga
     1261 cagcgtttcc caaaacaagg aaacctacac cgttacccgt gcggcagtta tcaacgaata
     1321 tcttttaatc ttgacggttg aagcgcaggt attgaaacgc ggcgagccgg tcggcaaacc
     1381 gatgaccgtg tccgtccgcc gcgtccttgc ttatgccgac aacgagatct tgggcaaaca
     1441 ggaagaggaa gcggcattgt gggcggaaat gcggcaggat gccgccgaac agattgtccg
     1501 ccgcctgacc tttctgaagg cggaatgacg tggcggcaca tatcggacac attgatacgg
     1561 acgcgccttt gaaacccctg tacgtcatcc acggcgagga agaactgttg cgtatcgagg
     1621 cattggacgc attgagggcg gcggcgaaga aacaaggtta ccttaatcgg gaagtttata
     1681 cggcagacaa tgccttcgat tggaacgaac tgttgcaaac cgcaggcagt gcgggtctgt
     1741 ttgccgattt gaagctgttg gaactgcata tccccaacgg aaaacccggc aaaaccggcg
     1801 gcgaggcgtt gcaggatttt gccgcccgcc tgccggaaga tacggtaacg ctggttttgc
     1861 tgcccaaact ggagaaaacc cagctccagt ccaaatggtt tgccgcattg gcggcaaagg
     1921 gggaagtgtg ggaagccaaa ccggtcggcg cggcggcttt gccccaatgg atacgcggac
     1981 ggctggacaa aatcggtttg ggtatcgggg cagacgcatt ggcactgttt gccgagcgcg
     2041 tggaaggcaa tctgttggcg gcgcgtcagg aaatcgacaa gctcgggctg ctgtatccga
     2101 aagggcatac cgtcaatatc gatgaggcgc aaaccgccgt tgccaacgtc gcccgcttcg
     2161 acgcgttcca actggcaggc gcgtggatga agggcgatgt cctgcgcgta tgcaggcttt
     2221 tggacggatt gcgggaagag ggcgaagaac cggtgctgtt gctgtgggcg gttgccgaag
     2281 acgtgcggac gctgatccgg cttgctgccg ccctgaagca ggggcagagt gtccaatccg
     2341 tacgcaacag tctcagactt tggggtgaaa agcagacgct cgcaccgctt gcagtcaagc
     2401 ggatttccgt cgtccgcctg cttgacgcgc tcaaaacctg cgcccaaatc gaccgaatca
     2461 tcaaaggtgc ggaagacggc gacgcatgga cggtattcaa acggcttgtc gtgtcgctgg
     2521 cggaataaag cggtaatccc caaaatccga aaatactgta aaataccgtt aatcctgaaa
     2581 agtattcacc aatccgtccg aaaacatttc agacggcacg accacctcaa taaaggaaca
     2641 ttaaccctat ggacaataag accaaactgc gcttgggcgg cctgatttta ctgactaccg
     2701 ccgttttaag cctcattatc gtattgattg tcgactcctg gccgcttgcc atcctgcttg
     2761 ccgccgtcat cgtcgccgcc gctgcgggcg gttttgtttg gacatcccgc cgacagcaac
     2821 gccagtttat cgaacgcctg aaaaaattcg acatcgatcc cgaaaaaggc agaatcaacg
     2881 aggcaaacct gcgccgtatg taccacagcg gcggacaaca ccagaaagat gcgattaccc
     2941 tgatctgcct gtcgcaaaaa tgttcggtgg acgaggcgca cgctatgttc aaaaaacgcc
     3001 cgacacgtca ggaaatcaat caaatggcgg caaaacagtc gcgcggtcag aaacgtccgc
     3061 accgttaacc gccgcaaggc atctttgcat aaatgccgtc tgaagcctgt tggcgtttca
     3121 gacggcatat tctgattgaa aagatgatga cactgaaaac cgccccgctc aaacgccgct
     3181 ttgccgccat gctgtacgaa atgctgctgg tcggtgcggc aacctgtttg gcagcattgt
     3241 ttgccggtat tgccgccatt tttctgaatc ccgtttctat cgcggtttct gcattggtaa
     3301 cgagtatcct gataatggga tcatggtggc tttattttcg cgccaactgg cacggtcagg
     3361 ggcagacctt ggcgatgagg acatggaaaa tcggcttgtg cgaccttaac ggcatacagc
     3421 cgtctttgca cctgctgcgc ctgcgcttta tttgggcgtg catattcatc gtcttcattc
     3481 ctatgcttgc ttatgccgga ttgcgccact tcctcggcat tccgcccaag ggcgcggtcg
     3541 gtgcggcatt gatttggctg attttaccgt gggggttcgc actgctgaat cccgatcggc
     3601 agtttctgta tgattttctt gcaggaacaa gattggtggc ggtcaaagga aagccttaag
     3661 cctttatacc gcaaaggttt caacctgaaa aaatgccgtc tgaaagggct ttcagacggc
     3721 atttgcttat cggggaaacc gattattcga tattctgcac ttgttcccgc atctgctcga
     3781 ttaagacttt cagttcgacc gaggcttggg tgcattcggc ggcaatggat ttgctgccca
     3841 aagtgttggc ttcgcggttt aattcctgca tcaggaaatc caagcgtttt ccgctgctgc
     3901 ctttgtgttc ggtaacgata cggcgcactt cggcaatgtg agtgcgcagg cggctgaatt
     3961 cttcgtcgat gtcggatttt tgaataaaga gggcaaattc ctgttgcagc ctgtcattat
     4021 tgatgtcgcc gactgcttcg acgaggcggg cgcggatttt ttctttatgt gtttccagca
     4081 gggtaggaaa gagttcgctc aatgcatcta tgatttcttc catgctttcg aggcgttgta
     4141 acaggtgctc gcccaatttt ttcccttcgc gccggcgtgc ggcagtaaag tcttttaacg
     4201 ctttttcggt cagttcggta atgctttttg ccaattcctc cgtattttcc ctttggcttg
     4261 ccaatacgcc ggggaaacgc aggatgtcgg caacgcccag ttttgccaaa tcgtgatgct
     4321 tgcggaggtc tttgttgatt tcggcaagct ggttcaccaa atccgagttg agttccaagg
     4381 actgtctgct gttttccgta tcttgaattt ggattttgca ttcgactttg ccgcgcgcga
     4441 tatgggatga aattttctcg cggataccgc tttccaaata gcgcaaatcg tcgggcatcc
     4501 tgatttgaat atctagaaaa cggtggttga ccgcacgcag gtcgaggttg atgcgtttgc
     4561 tgccgcactc tgccgccgcg ttggcaaagc cggtcatgct gtggatgtgg atatttccgc
     4621 tgctcatgtc gttctccgaa gcccgttaaa atggaatcaa tatatcacat ctgtatggcg
     4681 gcaagcgttt tcgggtgtga aaaattgaag gttttgcagc ggcagattgg aatcacgctc
     4741 ttttgttgct gcaaggaagg gaaatgtata gtggattaac caaaaccagt acggcgttgc
     4801 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
     4861 cgtacgattt gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat
     4921 atcaattccg ccaatctgtc ggaaaagcag ctgatgcggc agtgtctggt gcatgtctgc
     4981 tttttgattt cggcaattgc aacggcgtgg acggataaaa tcgtgtacgg cacgacgcac
     5041 aaaccgcatt gatgtttacc aaataaaata cccgacaaaa caatttgtcg ggtattttat
     5101 tgcgtatatt tcaaaccgct tcggcttctt cggtcaggaa accacgcagt ttctgcatgg
     5161 cttttgcttc gatttggcgg atgcgttcgg cagagacgcc gtattcggcg gcaagctggt
     5221 gcagcgtcag cccgccgtcg tcttgaagcc agcggctttc cacaatacgg cggctcctgt
     5281 catccagttg cgccaaagcg ttttgtaaac cttctgtttg cagggcgtaa tgcgcctgtt
     5341 tcgatagttg tcggctcggt tcggaatcgt ggtcggcaag ccagtcgatg ggggcgaaac
     5401 tgtcctcgtc gtcgctgttg tctgccatga tggcgatgtc gtgtcccgtc atgcgctgtt
     5461 ccatttccag aacttcggaa agtttgacac ccaaatcgtc ggcgatgtct tgtgcctctt
     5521 tgggagacag ggcgttgagg tttttacgca tactgcgtaa attgaaaaac agcttgcgtt
     5581 gcggtttggt ggtggcaacg cggacaaggc gccagttcct caggatgaat tcgtgaattt
     5641 ccgctttaat ccaatgcacg gcaaatgaaa acaggcgtgc acctcgacct ggctcgtagc
     5701 gtttgaccgc cttcatcagt ccgatattgc cttcctgaat caggtctgcc tgattcagcc
     5761 cgtagccgtc atagccgcgc gcgatggaaa cgacgacgcg caggtgggac aggatgagtt
     5821 gtttggcggc gttgaggtcg ccctttatcc ggcgttcggc gaggcgggtt tcttcttcct
     5881 gggacagcat cgggatgctg ttgacggtgt ggatgtattg ttcgaggctg ccgttgccgc
     5941 tttggatggc gggtaatgcg aaagcgttat tcatttgggg catttccttt cggctgaaac
     6001 tgcgtatcgg cggtttgctg tgttgggatg cagtatatca ctgcttggct tgtattttgt
     6061 atgtttggca ggagatatgc gctaaggttt gaaagacagg aaaattttgt aaggcaggtt
     6121 tgattgattt tgtaaacctg atggctcaat ccgatttcgg aattatatta catacgtagt
     6181 tgtatgtaaa cagccggttt gaaaaaagac agcccgtctg gacgggctgt gcaggtatca
     6241 gtgttctttg tttcggaaga tgaaaagaat cagtgcggct agggtcaata tgcccatcaa
     6301 ccaccatgaa ctgccggttt tcatataggg cgtttcgccg acatagcctt tgatgtgtcc
     6361 ttccaatacg gtttccgtat cgggttgggc ttgggcgatg atgttgcctt tgggggagat
     6421 gatggcggtt gcgccggtgt tggtggcacg gaccatatag cgtccgagtt ccatagcccg
     6481 tgcctgcgat tgttggaggt gctggtacat ggcgttggat tttccgtacc acgccatatt
     6541 gctggcattg gcaagcaggg tggcatcttt ggcggcggca atcagttcgt cgccgaatcc
     6601 gtcttcgtaa cagatgttga aggcgatttt ttggtttttc atcagcaggg cggattgctt
     6661 accgccgcct ttgcggaagt cggaaagggg catattcatc attttgtaaa gcggcgtggt
     6721 caggaagggc agcggtttgt attcgccgaa ggggacgagg tggtttttgg cgtagtaggg
     6781 gatgccgtcc tgattgtttt cctgataacc ggtcaggttg atgacggcgt tttcgtaacc
     6841 gttgccgtcc gaagtgtatt ggctgatgcc gacggcgagc gcgctgccgt tgttttgcgc
     6901 ctgttcggca aattttgcca gtatgttttc cggcaggttt tggcgcataa cggggatggc
     6961 ggtttcgggc aggatgacga tgtcggcagt ggttttgccg acctgttcgt aatatttctg
     7021 tatggtcggg atgacttggt cttcacgcca tttgagggtt tggtcgatgt tgccttgaag
     7081 cagggcgacg gtgctgcggc tgccgtcggg gcgggtgaag tcggtttgtc gggcggtgta
     7141 gcctgcggca agcagggcgg caatcagggc ggcaatcagg ataattggaa gcaggcgttt
     7201 gcccgaacgt gtggtgttat cactcgccaa aaccagccag acaccgagaa aggcggttgc
     7261 cagtgtaacc atgtggatgc cgcccaatgg ggcaaagccg gcgagcgggc tgtccggggt
     7321 gatttgggag tagccgattg cgccccagcc gaatccggtc aggaaacgtt cgcgggcaaa
     7381 ctcggtcagc gtccacagga tgggcagtac caaaccgatt tttatgcccc gaggcagggt
     7441 aaattttttc cacagccaga aacacagtgc cggataaagg gcaaggtagg cggggagtag
     7501 gaaggtcagc ggtacggcat agaggtcggg caggccggaa acgtcgtgca gggcggtgtg
     7561 tatccagtag aactgtgtcg tgtatgcggt caggccgaac aggtaggcgg aagagacagc
     7621 aaaacgcgga cgcagttcga tgaggcggac gaaggcaccg aaaatcaagg gcatcagcca
     7681 aaagtggtag tagggtgcga aggtaaaggg ggtggcggcg gcaaaaagga tgagcaaagg
     7741 ccagtagagg gcggggtgct gccagtattt gtccagtttg gaaaccatat tcatctgtct
     7801 gttcggaaga taccgtctga acatctttca aacggcatcg gtatttgaaa aagaaatcaa
     7861 tgcctgccga aacgattcat cagcggcaag gcgggggcgc aggcaatcga accggaaagc
     7921 agcccgacaa cgaggttggc gaagtactcc gcccagccag cgtcccagtg ttgcgcgtgc
     7981 aggaaatcgt gcagaaggcc gaggttgtgg gcgaccagta cgccgccgat aagaaacatg
     8041 gcaagcgtgc cgaccacgct caaaccgcgc ataaagcaag gcataaaggc agtcagcatt
     8101 tgccccaaac tgcgcgaaaa ggtttgtggg cggcgcatca gcagcatgcc taagtcgtcg
     8161 agtttgacga tgacggcaac gattccgtac accaaaacag tcatgccgat gccgattgcc
     8221 gccattacga gcatgcgcgt catgtcggtg cagaaacttg tgcagcagct tttctacgcc
     8281 gtcaaagcac agataaatgc cgcctgccgt caaaagcggc gtaatgagtt gcggcaggaa
     8341 ggcggaaagc agcagagccg caggtaccaa aaccggcttg ttggaaaaag aacctttcgc
     8401 catcgaccaa acaatcggca actcgcgttc tgccgatacg cccgtaaccc ggttggcatt
     8461 gggtgccaaa tcgtcgccga ccatgccggc ggttttcttt gcggcggctt tggtcatcag
     8521 ggcaacatcg tccaaaacgg cggtgatgtc gtccggcagg gtaaatagtg aggcaaatgc
     8581 cattaaagaa tcctgaaatg cggcgcaaag tccgacatta tataggagaa cgcggatttg
     8641 ggcggtttca ggcggcatga aacaggaaaa tgccgtctga aggcggtggc ggacgtgaag
     8701 taaagtttcg tgaaaaaaat gccgtgttac agtctttcga ttttaattta atgaatttta
     8761 agggagaatc gttagcgtgg attggatggg cagtctgttc ctgccgggtg gcgcactgtt
     8821 gtttctgagc gtggtttcga ccactttacc cgcacgtttg ggaatgcctt tgctgctggt
     8881 ttctcccgcc ggcgtgttgg acggggcggc ggaagccttg gcgattgcgg cgttcctgat
     8941 gctggtcgcg cgtccgccgg cagtgttcgg cggtttgtgg aaattcaatt acagcctgcg
     9001 tgaaaaggcg tatagccgaa tcgaaatgca gtccgacacc gtgcttcagg cggggatttg
     9061 gtgtggtaca tcctgcccga cggcaaggtc g
//
LOCUS       DAWFKN010000076         8824 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFKN010000076 DAWFKN010000000
VERSION     DAWFKN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.076"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGR46_001725"
     CDS             complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGR46_001725"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0856613.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1355..2485
                     /locus_tag="WGR46_001726"
     CDS             1355..2485
                     /locus_tag="WGR46_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0856614.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2556..3407
                     /locus_tag="WGR46_001727"
     CDS             2556..3407
                     /locus_tag="WGR46_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0856615.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3490..4152)
                     /locus_tag="WGR46_001728"
     CDS             complement(3490..4152)
                     /locus_tag="WGR46_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0856616.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            4186..4293
                     /locus_tag="WGR46_001729"
                     /pseudo
     CDS             4186..4293
                     /locus_tag="WGR46_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4352..6145)
                     /locus_tag="WGR46_001730"
     CDS             complement(4352..6145)
                     /locus_tag="WGR46_001730"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0856617.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAKEYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNVAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6391..7500
                     /gene="proB"
                     /locus_tag="WGR46_001731"
     CDS             6391..7500
                     /gene="proB"
                     /locus_tag="WGR46_001731"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0856618.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIARIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7513..8775
                     /locus_tag="WGR46_001732"
     CDS             7513..8775
                     /locus_tag="WGR46_001732"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0856619.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         2011 a         2483 c         2297 g         2033 t
ORIGIN      
        1 ccgataagtt tccgtgccga caggtctaga ttcccgcctg cgcgggaatg acgaaatttc
       61 aaagttatgg cgttatcgga aaaacagaaa atcaaagccg gagaatttat cccaaacaac
      121 cggatttcaa aaaaaccaga ttcccgcctt cgcgggaatg acggatttta ggtttctgtt
      181 ttggtttctg ttttcgcggg aataacaact tagaaattgc ccgaaacccc aaaaacagaa
      241 accggacaaa cggggtttcc gccaaagccg gcattttccg actttgcttc aggcatcttc
      301 cccaccgccc aaaatcatcg aaccgatacc ggcatcggta aagatttcca gcaaaagcgc
      361 gttgggcacc ctgccgtcga tgatgtgcgt ggctttcacg ccgttgacgg cggcttcgac
      421 cgcggaagcg attttcggca gcataccgcc atacagcgta ccgtcggcaa tcagttcatc
      481 aatccgtttc ggcgtgagtt tggtcagcag attgcccgtt ttgtccatca caccggcgat
      541 attcgtcatc atcaaaagtt tttcggcgtt caattcttcc gccaatttgc ctgccaccaa
      601 atcggcgttg atgttgaacg cttcgccttt ttcacctacg ccgacggggg cgacgacggg
      661 aatgcagcca cgttctatca gccctttaac caaaccggta tcgatgcttt ccaccgtacc
      721 gacctgtccg atgtccacgc cattctgttc gggcgtatca atcaaaagtt tcttcgcctt
      781 aatgaaatgg tcgtcgcgtc cgcttacgcc gaccgcgtgt ccgccatatg tgttaatcat
      841 cgacacgatt tctttattga catgcccgcc caacaccatt tcgacaatat ccatcgcctc
      901 tttgtcggta acgcgcattc cttggacaaa ctcacccttt ttgccgactt tttcaagcat
      961 cgcattgatc tgcggcccgc cgccgtgaac gatgacggga tgaatgccga ccagcttcag
     1021 cagcacgaca tcgcgggcaa acccttcttt caaggcaggt tcggtcatcg cgttgccgcc
     1081 gtatttgatg acggcgaccg aaccggaaaa ccggcggatg taaggcagcg cttcggcaag
     1141 gatacgcgcc ttgtcggcgg cggaaataat gttttcagac tccataacgg ctccctattg
     1201 ttgacaattt cggcttatgt taacgccaaa cgccgtctga aacaatcttc agacggcatc
     1261 cggcagactt tcgggcaggt tgaagccttg cgatacgggc ggtaaaatcg cggtttatcc
     1321 ggttcgagaa accggcgaca gcaaggactt tcccatgatt aaaatcagca cccgcttcga
     1381 tgccggctcg gtcgtcgtca aagacctgac cgatccttcc aacatccgcc tcgccctgcg
     1441 tcccgacaac gcctccgatt ttgcacaatg gttctacttc cggctgcaag gcgcggcata
     1501 tcaaaactgc atcatgcatt ttgaaaacgc ggcagaagcc gcctacccga aaggctggga
     1561 aggctatcag gcgtgtgcct catacgaccg ccgcaactgg ttccgcgtac cgacttccta
     1621 cgaaaacggc gtactgaccg tcaatcatac gccgctgtcc aacagcgtgt attacgccta
     1681 tttcgaacct tactccgaag aacagcattt aaacctcctc ggcgacgcgc aaggcagcgg
     1741 cctgtgccgc atcgacgact tgggcagcac cgtgcaaggg cgcgacatca atctgctgac
     1801 catcggcaat caggtcgaaa gcgatctgaa aatctggatt accgcccgcc agcatccggg
     1861 cgaaaccatg gcggaatggt ttgtcgaggg gctgctcggc aggctgctcg atccgcaaga
     1921 cccgacagcg cgcaccctgc tcgaccgcgc cacgttctat atcgtcccca atatgaaccc
     1981 cgacggttcg gcactgggca accttcgtac caacgccgcc ggtgcgaacc tcaaccgcga
     2041 atgggaaaat ccgactttgg aaaaaagccc cgaagtgttc ttcgtgcgtg aaagaatgct
     2101 ggaaaccggc gtggatttgt ttttggatat ccacggcgac gaagggttgc cctttgtctt
     2161 tgttgcagga actgaaggcg tgccgaacta caatccgcgc atttccgcgt tggaaacaca
     2221 gttcaaaacc gcccttttga acgccagccc cgatttccaa gacgaatacg gttacgaaaa
     2281 agatgcgccc ggtcaggcaa acatgacctt ggcaacaaac tgggtcggca accgtttcaa
     2341 ctgccttgcc tatacgttgg aaatgccgtt caaagacaat gccaacctgc ccgacgacga
     2401 tttcggatgg aacggtcaac ggtcgttgcg cttgggcgaa gccgcgttgt ccgccatcct
     2461 caacatcatc ggcgatttgc gctgaaaccc ttattgcaga aatgccgtct gaagcatcat
     2521 gcctttcaga cggcatttga aaacgaaagc ccgttatgat tacccatccc caattcgacc
     2581 ccgtccttat cagtatcggc ccgcttgccg tccgctggta tgccctaagc tacatcctcg
     2641 gatttattct ttttaccttt ctcggcagaa ggcgcatcgc gcaaggcttg tccgttttta
     2701 ccaaagaatc gctcgacgac ttcctgacat ggggcatttt gggcgtaatt ttgggcgggc
     2761 gtttgggtta cgtcctgttt tacaagtttt ccgactacct cgcccatccg cttgatattt
     2821 tcaaggtatg ggaaggcgga atgtcgttcc acggcggctt tttgggtgta gttattgcca
     2881 tatggttgtt cggccgcaaa cacggcatcg gcttcctcaa actgatggac acggtcgcac
     2941 cgctcgttcc gctgggtctc gcttcgggac gtatcggcaa cttcatcaac ggcgaacttt
     3001 ggggacgcgt taccgacatc aacgcatttt gggcaatggg cttcccgcag gcgcgttacg
     3061 aagatgccga agccgccgcg cacaatccgc tttgggcaga atggctgcaa caatacggta
     3121 tgctgccgcg tcatccctcg cagctttatc agtttgcact tgaaggcatc tgcctgttcg
     3181 ccgtcgtttg gctgttctct aaaaaacagc ggccgaccgg acaagtcgcc tcgctcttcc
     3241 tcggcggcta cggcatattc cgcttcattg ccgaattcgc acgccaaccc gacgactatc
     3301 tcgggctgct gaccttgggg ctgtcgatgg ggcaatggtt gagcgtcccg atgattgttt
     3361 tgggtatcgt cggctttgtc cggttcggca tgaaaaaaca gcactgaccg cctgtgaaaa
     3421 ataccgtctg aaacatggaa atccggtttc agacggcatt tttacgtttg gttcatggaa
     3481 acaaacgctt caattcttac cgcaacacgc cttgtatttt ctgccgctgc cgcacggaca
     3541 gggatcgttc ctgcctgttt tttcgccttc cctgcggacg gtttgcggtt tgttgatgac
     3601 tgcctgccag tagcggtaga tatccgccaa tgcataaggc aactcggatt ccagttccgc
     3661 cagctcgcct tctgtgaatt gcagacggat agcgccgttt tcctcttcgt cgtaaatacc
     3721 gcccaatgcc atgatgggat aaaacaactc ttcaaacgct tcatcatcga cggcttcaaa
     3781 ccaatcggtc ggcacaatat ccaaaccgta aagataagca ttgcaccatg tgtaaaaatc
     3841 gctgccgccg tcttcgtttt catacagcca caaatcgggc agttttttat ccgacatcgc
     3901 ggcggttgtt tccatcgcca ttgccaaaac cagccgttcg atttcggaac gttcggcggc
     3961 ggtaaattgc gattcgtcgc ccaacacttc gggcagccag tcgagcggtg tcaatttgtc
     4021 cggcccgctc aacagcgccg tcataaaacc ttgaacctcg tcgcaacgca tcgtgttgcc
     4081 ttgttcgctt ttggcatcca acaattcgct caaccgccgt ttggatgctt cggtaaattt
     4141 tcgggaatcc atcattttcc ttttcaaatg ggttttgcgc cctattatag tggattaaat
     4201 ttaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
     4261 acgccgtact ggtttaaatt taatccacta tatcgccgca atgccgtctg aagccttttt
     4321 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4381 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcagtggcaa
     4441 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgt gccagacgga
     4501 cgctggtttc gccttggctt tccgtacctt gcgtgacggc attgacggaa taaatctgca
     4561 aagtcgcgcc gctttgcgcc acgctttcaa tcgctttgaa aattgcatca accgggccgg
     4621 aaccggttgc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4681 gctcttcgcc ggtttcggta ctgatttttt gggaaatgaa tttgtagctt tcggtattca
     4741 tattgcccat ttcgtccgac accagcgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4801 tgtcggcgag ttctttgaag cgtgcaaatg ccacgttcag tgcctcttcg ctttccaact
     4861 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4921 tcaatcggtt agccgaccaa ccgaccgatt cggcagacat aatctcgtaa gtttcgcggt
     4981 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     5041 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     5101 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     5161 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     5221 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     5281 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5341 gttcgcggaa aaattcttcg gttttgtacg ggatggaata gccgacggta tcgggaatat
     5401 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcagcaagg aaatcgattt
     5461 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattct ttggcgattt
     5521 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5581 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5641 cctcccccgc ctgacggatg tcccgctcga tggcgcgtga caatgaacag accgttgatt
     5701 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5761 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5821 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5881 tgataacgcg gttggcctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5941 aagcattcaa atccagcggc ctgccttgcg attcggcaag cgcggtcagc ttttgcagtt
     6001 gcgccaaagg cagcgacccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     6061 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     6121 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     6181 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaaatc
     6241 tcaataaacc gccatagatt ttattaattt tatacatttt gtccatttta ttgcagacgc
     6301 ggcgtgattg ttgacaattg cggcagaaca gggcaaattc ggcggcacaa ggtggcaacg
     6361 agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt atttaaagtc
     6421 ggcacatctt cgattaccca ttcggacggc agtctctcgc gcggcaaaat ccaaaccatc
     6481 acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt gtcttccggc
     6541 gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac gtccggtcaa aatcgccgac
     6601 aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac ggcaaacctg
     6661 tcttcagacg gcatcgtgtc cgcgcaaatc ctgctcagcc gcgccgactt tgccgacaaa
     6721 cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc tgcaacgccg cgccgtcccc
     6781 atcatcaatg aaaacgatac ggtttcggtt gaggaattga aaatcggcga caacgacaca
     6841 ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct gaccgacata
     6901 gacggtcttt acacgggcaa cccgaacagc aatcccgatg ccgtacggct ggacaaaatc
     6961 gaacacatca accatgaaat catcgaaatg gcgggcggat cgggttcggc aaacggcacg
     7021 ggcggtatgc tgaccaaaat caaagcggca accatcgccg ccgaatccgg cgtaccggtg
     7081 tatatctgtt cctcgctcaa acccgatgca cttgccgaag ctgccgaaca tcaggcggac
     7141 ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc agaagcaatg gctggcgttc
     7201 tattccgaaa gccggggcag cgtttatgtg gacgaaggtg cggaacacgc tttgtccgaa
     7261 cagggaaaaa gcctgctgat gtcgggcatt gccagaatcg aagggcattt ttcccgtatg
     7321 gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg gcgcgtcctg
     7381 ttcggttctg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg cgtgttcatc
     7441 catcgggacg actggatttc catcacgccc gaaatacgcc tgcttctgac cgaattttag
     7501 aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc ggcaaaaaaa
     7561 tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc tgcttgctat ggcggacagt
     7621 ctggaagcgg cggcggcaga tattttggcg gcaaaccgtc aagacttgga agccgcggca
     7681 ggcaacattc ccgaaagcat gaccgaccgc cttttgttgg acggcaaacg catttgcgcg
     7741 atggcggacg gcattagggc ggttaccgca ctgcccgacc ccgtgggcga aatactggaa
     7801 acctcgactc tgcccaacgg cttggaaatc gtcaaaaagc gtgtggcgat gggcgttatc
     7861 ggcattattt acgaaagccg cccgaacgtt acgtccgatg cggcggcttt ggcactgaaa
     7921 agcggcagcg cggtcgtact ccgcagcggc aaagatgcat tccaatccgc acgcgccatc
     7981 gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgacgc gctccagttg
     8041 attgaagaca ccgggcgcga aagcagttac gaaatgatga gggcgaaaga ttatctagac
     8101 ctgctgattc cgcgcggcgg ggcggggctg atacgggcgg tggttgaaaa tgccgtcgtg
     8161 ccggtcattg aaacgggaac gggcatcgtc cacatttata tcgacaagga cgcggattgg
     8221 gacaaggcgc tccgtatcgt gtacaacgcc aaaaccagcc gcccgtccgt gtgcaactcg
     8281 atggaagtgc tgctggtgca tgaagacatt gctgccgact tcctgcccaa gctcgaacgg
     8341 ctgttggttc gcgaccgcat agaagccgga ctgccgcccg tccgcttccg tttggatccg
     8401 caggcggcgc ggcatatcgg cggcgaagcg gcgggtgcag acgatttcga taccgagttt
     8461 ttagactaca tcctcgctgt gaaaaccgtc gcatcggtcg aagaggcggt cgggcacatc
     8521 gaagcccacg gcacacacca ttcagacggc atcgttaccg aaaaccgcca cgctgcggac
     8581 tatttcacga cccatatcga ttctgccgcc gtgtatgtca acgcgtccac gcgctttacc
     8641 gacggcggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa actccacgcc
     8701 cggggcccga tgggtttgaa agagctgacg agttacaaat acatcgtaca aggcacggga
     8761 caggttaggg aataatccga attcaaacgt ctgatatagt ggattaactt taaaccgata
     8821 cggc
//
LOCUS       DAWFKN010000077         8788 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFKN010000077 DAWFKN010000000
VERSION     DAWFKN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.077"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(391..825)
                     /locus_tag="WGR46_001733"
     CDS             complement(391..825)
                     /locus_tag="WGR46_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0856620.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR46_001734"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR46_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0856621.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGR46_001735"
     CDS             complement(1556..2398)
                     /locus_tag="WGR46_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0856622.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGR46_001736"
     CDS             2653..3291
                     /locus_tag="WGR46_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0856623.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WGR46_001737"
     CDS             complement(3743..4543)
                     /locus_tag="WGR46_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0856624.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WGR46_001738"
     CDS             complement(4679..5182)
                     /locus_tag="WGR46_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856625.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WGR46_001739"
     CDS             complement(5519..6160)
                     /locus_tag="WGR46_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0856626.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            6489..8702
                     /locus_tag="WGR46_001740"
     CDS             6489..8702
                     /locus_tag="WGR46_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0856627.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPIPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2344 a         2366 c         1895 g         2183 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttatacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcgaaataa agtggcacgc ccgatttttc actcataccg attaaataat
     5581 ccatcgtctt ccgagggaac gcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacaa cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc tgagaccttt gcaaaaatag tctgttaacg aaatttgacg
     6301 cataaaaatg cgccaaaaaa ttttcaattg cctaaaacct tcctaatatt gagcaaaaag
     6361 caggaaaaat cagaaaagtt ttgcattttg aaaatgagat tgagcataaa attttagtaa
     6421 cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc ggaacggaag
     6481 accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgact ctgcaagaat
     6541 tgcgcgaatg gttcgacagc tactgcgccg cgctgccgaa caacgataaa aaacttgtct
     6601 tagccgcccg ttcgctggcg gaagcacatt accccgccga tgccgccacg ccgtatggcg
     6661 agccgctgcc cgaccacttc ctcggcgcgg cgcaaatggt tcatgaactc gacctgctcc
     6721 ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc gactggaacc
     6781 tattggtttc cgaacgctgc aacagtaccg tcgccgagct ggtcaaaggt gtggacgaag
     6841 tgcagaaact cacccacttc gcccgggtgg acagcctcgc cacaccggaa gaacgcgccc
     6901 agcaggcaga aactatgcgg aaaatgctgc tggcgatggt taccgacatc cgcgtcgtgt
     6961 taatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgcc cccgacagcc
     7021 ccgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc gccaaccgtt
     7081 tgggcgtgtg gcagctcaaa tggcagctcg aagatttggg cttccgccat caagaacccg
     7141 aaaaataccg cgaaattgcc ctgcttttgg acgaaaaacg caccgaacgc ctcgaataca
     7201 tcgaaaactt cctcaatatc ctgcgtacgg aactcaaaaa atacaatatc cactttgaag
     7261 tcgccggccg tccgaaacac atctactcca tttacaaaaa aatggtgaag aaaaaactca
     7321 gcttcgacgg cctctttgac atccgcgccg tgcggattct gattgatacc gtccccgagt
     7381 gttacaccac gctgggcatc gtccacagcc tctggcagcc cattcccggc gagttcgacg
     7441 actacatcgc caaccccaaa ggcaacggct ataaaagttt gcacaccgtc atcgtcggcc
     7501 cggaagacaa aggcgtggaa gtgcaaatcc gcaccttcga tatgcaccaa ttcaacgaat
     7561 tcggtgtcgc cgcccactgg cgttacaaag agggcggcaa aggcgattcc gcctacgaac
     7621 agaaaatcgc ctggttgcgc caactcttgg actggcgcga aaacatggcg gaaagtggca
     7681 aggaagacct cgccgccgcc ttcaaaaccg agcttttcaa cgacacgatt tatgtcctga
     7741 ccccgcacgg caaagtcctc tccctgccca cgggcgcgac ccccatcgac ttcgcctacg
     7801 ccctgcacag cagcatcggc gaccgttgcc gcggtgcgaa agtcgaaggg cagattgtgc
     7861 cgctgtccac cccgctcgaa aacggacagc gcgtcgaaat cattaccgcc aaagaagggc
     7921 atccttccgt caactggctc tacgaaggct gggtcaaatc caacaaggca atcggcaaaa
     7981 tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc cgcgtccaac
     8041 tcgacaaaca gcttgccaaa ctcacgccca aacccaacct gcaagagctt gccgaaaatc
     8101 tcggctacaa aaagccagaa gacctctaca ccgccgtcgg acaaggcgaa atttccaacc
     8161 gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccataccc gtcagcgaaa
     8221 ccaccatcgt caaacagtcc aaaatcaaaa aaggcggcaa aaacggcgtg ctcatcgacg
     8281 gcgaagacgg tctgatgacc acgcttgcca aatgctgcaa acccgcgccg cccgacgaca
     8341 ttgtcggctt cgttacccgc gatcgcggca tttcggtaca ccgcaaaacc tgcccctctt
     8401 tccgacacct cgccgaacaa gcacctgaaa aagtgctgga cgcaagctgg gcggcgttgc
     8461 aggaaggaca agtgttcgcc gtcgatatcg aaatccgcgc ccaagaccgc tccgggcttt
     8521 tgcgcgacgt atccgacgca ctcgcccgcc acaaactcaa cgttaccgcc gtgcaaaccc
     8581 agtcccgcga cttggaagcc agcatgaggt tcacgctcga agtcaaacaa gttaccgacc
     8641 tcccgcgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt acccggcttt
     8701 aaatacaaaa atgccgtctg aaagccgtat aacgcttcag acggcatttt gattaccagg
     8761 gtttgctatt ttttgttgca tagtcaat
//
LOCUS       DAWFKN010000078         8767 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFKN010000078 DAWFKN010000000
VERSION     DAWFKN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.078"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(288..803)
                     /locus_tag="WGR46_001741"
                     /pseudo
     CDS             complement(288..803)
                     /locus_tag="WGR46_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1811..2581)
                     /gene="xth"
                     /locus_tag="WGR46_001742"
     CDS             complement(1811..2581)
                     /gene="xth"
                     /locus_tag="WGR46_001742"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0856628.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2641..2922)
                     /locus_tag="WGR46_001743"
     CDS             complement(2641..2922)
                     /locus_tag="WGR46_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0856629.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            complement(3328..4209)
                     /gene="nadC"
                     /locus_tag="WGR46_001744"
     CDS             complement(3328..4209)
                     /gene="nadC"
                     /locus_tag="WGR46_001744"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0856630.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(4284..5222)
                     /locus_tag="WGR46_001745"
     CDS             complement(4284..5222)
                     /locus_tag="WGR46_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856631.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5529..6641
                     /gene="nadA"
                     /locus_tag="WGR46_001746"
     CDS             5529..6641
                     /gene="nadA"
                     /locus_tag="WGR46_001746"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0856632.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKTEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            6693..8201
                     /gene="nadB"
                     /locus_tag="WGR46_001747"
     CDS             6693..8201
                     /gene="nadB"
                     /locus_tag="WGR46_001747"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0856633.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLNRQTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPTLQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYARRQNIGAHFNSDC"
     gene            8384..>8767
                     /locus_tag="WGR46_001748"
     CDS             8384..>8767
                     /locus_tag="WGR46_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856634.1"
                     /translation="MSTFFRQTTQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNRLAQDNTLSELLELINRQL"
BASE COUNT         2037 a         2430 c         2307 g         1993 t
ORIGIN      
        1 aaataactga aaccgaacag actagattcc cgcctgcgcg ggaatgatga attcatccgc
       61 acggaaacct gcaccacgtc attcccacga acctacattc tgtcattccc acgaaagtgg
      121 gaatccagga ttcagggacg gagaaacctt ttttcccgat aagtttccgc accgaccggt
      181 ctagattccc gcctgcgcgg gaatgacggt tcgggtattt ctgacgattc atcgagccgt
      241 agcaactgta tttttcaccc cgtcgggcaa aaataccaaa actcaaatca agccgtccgg
      301 ataccgtttt cggcggtatc gttttcggca aaataatcac gcatccgggc attcgatatc
      361 gtcagcagtt tgcgcataca tgccgtaacg gcaaccttat acggcttacc ctcggacagc
      421 aggcgttggt agaaatcccg aataagcggt tcaaaacgtg tcgctgccac ggtagccata
      481 tacagtgcct tacgcaccgc agaccttccg ccaaagcagc ggcttttaaa tttggtttcc
      541 ccgccctccc tcgggtgcgg ggcaatgccg gccaaactcg ctatccgttt gtgcgacagc
      601 cgccccaatt cgggcagcat cgccatcagc gtagccgtcg ttatcgaacc gatgcctttg
      661 atttgctccg ccacttgggc tttgccgtca aaatgcgtgt gggtgtggtc gtcgatttgt
      721 ttgtccaatt cgtcaatcag ccggtcaaaa tgggcaatca gttgtttgac gcttccgact
      781 tgcgtttcat gaaactgatg cagatatagt ggattaacaa aaatcaggac aaggcgacga
      841 agccgcagac agtacaaata gtacggcaac gctgtactgg tttaaattta atccactata
      901 ttttgtaata gctgccatta tgcctactag gatatatacc ttctatataa tccttattta
      961 aataaaaacc cttataaaaa tctgtgttat ctgtcgggat tgaaaaaaag attttatctt
     1021 tctcatcagg tttatatttg ctatcaatat caaaaagaga ataaggatta caatgagttc
     1081 gacatactag gtaaaaattt atatcttgga tcatgaaata ttcgatattt atcaggatcg
     1141 aaagatgcta aaaattcatc aatatcggta ctggatattt tgtcatcact aataaacatc
     1201 gctagagatg ggcgactttg attttccatt tgttgattag taaaggatat aaatactaac
     1261 ttgccattgt caaaccacat agatttatat agtggattaa atttaaacca gtacgtcgtt
     1321 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     1381 ttaatccact atagttattg cctttgggag aatcaattct gacagcacat aacataggtt
     1441 catctttaca gtcgttatat tgggtttcat caaactcaac tgcatagcac aataaaggca
     1501 ataggaaaaa aaggagatta atttattact atacataagg cagtctgact attcattcta
     1561 aaataaatcc ggtttttaat atttgattct gctccggatt atatacataa taaattttac
     1621 tgctccaaaa tctgatatta accctatgga tttcataaat ttttcagaaa aataggctgt
     1681 gcttgaacaa tccgccgact ccggctgttt gtcagggaaa aggcaattat tttcggttag
     1741 tcagcagcct atcctattga atcacttaaa gatatggatg tttgtcaacg ccatatcaaa
     1801 acacggtctt ttaccaatcg aattctgccg tcaccggcgc gtggtcgctc ggacgctcca
     1861 gcgcgcgcgt ctccaaatcg acacggacat ccttcaacgc ctccgccatc gcaggcgaca
     1921 ccaaaatatg gtcgatacgc aggcccagtt tgcgttggaa catcgcgccg cgatagtcga
     1981 accaggtata gaacgcgcct tcgggatgga cttggcgcag gctgtcggtc agtcccaaat
     2041 ccagcaggtt tttgaaccac tgccgttcga cggacgaaca gtggattttt tcgtgccatt
     2101 tttcagggtc gtaacagtcc gcatcggcag gcgcgatatt gaaatcaccc agcagcacca
     2161 gtttgccgta gcgggtcatt tcatcgcgga caaactccgt cagtgcggca aaccattgtt
     2221 ctttatattt gaatttcggg ctgtcgaggg cttcgccgtt gacgcaatag acattgatga
     2281 cgcgcacacc gccgacggtt gccgcaatca cgcgccgttg cggatcgtcc ggcagcgcgg
     2341 gcagcccgat atgcacgtct tccggcgcgt tgcggctgac gattgccacg ccgttgtagg
     2401 ttttctgccc gctccaaaca cagtgccagc ccatcatttg caaagcggcg gccggaaatt
     2461 tgtcctgatc gagtttgagt tcctgcaaaa ccaaaatatc gggcggattg tcggcaagca
     2521 ggttttgcac ctgcggcagc cgcacattga gcgaattgac gttccaagtg gtgattttca
     2581 taacatttcc gaaaaatgcc gtctgaaacg ttcagacggc aaaaggaaag gattttaaca
     2641 ctataccgcg cgtttgtttt ccaaatcgcg gattgtccgc agaatcgttg ccgcgtcttc
     2701 ggaaatcagg cggtattcga taatgcggtg gtaacgcgta aagtccacca gaccgcccgc
     2761 cctcaggcgg ttcaaatggc cggaaacggc ggtggccggc agggataagg attttgccag
     2821 ttcggcgata ttgcgttcgc tgtccaacag ttggatcagt atcgccatac gttcgggatg
     2881 cgccataagt ttgagtatgg tgtggagcgg tattgtgttc atcggtattc ctaaaacctt
     2941 tgccgcccgc caagcggatt gacgggcgac agatatggat gattgtgatt atctttataa
     3001 atcttattat aaatcaaata tttatggatg attgtacagg attgtatgtt ccttttccca
     3061 ctttttttgc aaacacgcgt aaaggcgggc tttcagacgg cataaaactg ccgcccccgt
     3121 cctttcccct gccctaccga tgcctccgcg ctgtcgggca aacaaaatac ctgcctgaaa
     3181 cgctgttttt tctaaagcat cacgaccttc acgaaaaatg ccgtctgaag aatatttcag
     3241 acggcatttc ctccttgagg caataaccgg attacggtta ggcggttcct tgtctgacat
     3301 atcagacaac tgccgacacc ctaaaactca tgccaagaaa tccaaaccta tgtccaacga
     3361 gcggctgctg tgggtcagat agccgagggc gatgccgtcc acgccggttt gcgccacgcg
     3421 cttcaggcgg tcgaagccga tgccgcccga tgcttcgcaa tagacggtgt gggggtgggc
     3481 ggtttgcgtg tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttgtccag
     3541 caaaatccgt tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tgtccacttc
     3601 gatttccacg caggtcaatg ctccgaccgc ctgttttgcc tgccgaacgg cttgggcgat
     3661 gctgccgcaa taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca agcccatgcg
     3721 gtggttcacg ccgccgcctg ccctgacggc gtatttttgc aggacgcgca gcagggggat
     3781 ggttttgcgg ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc
     3841 ggtggcggtg gcgatgccgc ttaagtgcgt gaggtagttg agcgcggtgc gttcggcggc
     3901 gagcagcgcg cgggcgttgc cttcgacggc agccagcgtc tgacctgcgc ggacgacttg
     3961 cccgtctcgg atttcggctt ggaagcgaac ggacggatcc atcgtctgaa aggcgaggcg
     4021 cgccaagtcc atgccggcga taacgccgtc ttcgcggctg acgaggaaga gtttggcggt
     4081 tttgtcgggg gcgataacgg cggcggacgt aatatcgccg cgcctgccca aatcttcgct
     4141 caaggcttgt tccaccatgg ggcgcaacag ggtgtcgggc agggggaaga gggtgttttc
     4201 agacggcatg gggtttcctt tgttgaaaaa gattgatgtt gcaccgatgg gcaaaacttc
     4261 gtttgatgtc gtctgaaaac gggctaacgg ctgcccagcg gcagtccgat gtccgaaatc
     4321 agcctgtcgg gcaggacgtc gtcgcggaag cggcaaagct gcgcgggacg gcctttgctg
     4381 cccgatacac cggtatccga cggctcgatg aggttttgct gctgaatctg gcggcggaag
     4441 ttttgcttgt gcagcaatct gccgctgatg gcttcgacac tgttttgcag ttgcagcagc
     4501 gtgaattcgg gcggcatcag ttcaaaaatc acggggcggt atttgatttt ggcgcgcagg
     4561 cgagacaggg cggtcgccag tacgcggcgg tggtcgtggc gcatgggctg ccccgtaagc
     4621 gcgaagtcga agtttgcctg cggctcggcg gcctccgcta tcaggccgct ttcatacagc
     4681 atttcatagc gttgcaaaac gtattcttcc gaccagtttt ccggttcgac cccccaacac
     4741 aaatgaatgc gcttgagccg cttttggcgc acttcctccg tgtccgccga gtttgcccaa
     4801 atgcgcaggc ggccgacgac ggcgtcgcgc tgcccgccgt cggtacgcaa atcttcccac
     4861 gggaaatagc cgtagcagtc ctgccatttc gcgtcgggat gcaggatgct gtcggctgcc
     4921 tcgcgcacca gccccaaata gctgacgtac aagacgggca tgccgtgttc gttgcggcgg
     4981 tgggtatcga caaaggtgta aagctgttcc acatagccca taggctgcga agtctgcttg
     5041 gcgacccaca gtttcacgcc cgcctgcagg gaattgcgca ggggggagag cgggccgttg
     5101 ggcagaagcg tgccttgggc gacggtcaat acccgcaggc cgccgtcggt aacggcaatc
     5161 aatacgggaa ccagctcgac gatgctttgc ggcggggctt cggcttcggg gtaggcatcc
     5221 atgctcggct ttctgcgggt gggaaaggct ttattgtata gtggattaaa tttaaaccag
     5281 tacagcgttg cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     5341 tgatttttgt taatccacta taaaccaaag cggtttgtcg gaacgaagcg ggtcggtttt
     5401 acggtatcgc aaacctgttt tcagacgacc ttgcttatta tactcaattt gagcataata
     5461 taaaaatatt tcaaaatata aacggcaaat ctttatgccg tctgaaaaac cagccataag
     5521 gaagtctcat gcaaaccgcc gcccgccgct cgttcgatta cgatatgccc ctcatccaaa
     5581 ccccgacttc cgcctgccaa atccgtcagg cgtgggcgaa ggttgccgac acgcccgacc
     5641 gcgagacggc aggccgtctg aaagacgaaa tcaaggcttt gctgaaggag aaaaacgcgg
     5701 tcttggtggc gcattattac gtcgatccgc tgattcagga tttggcttta gaaacgggcg
     5761 gatgcgtggg cgattcgctg gaaatggcgc gcttcggcgc ggaacacgaa gctagtacat
     5821 tggtggtggc gggtgtgcgc ttcatgggcg agagcgcgaa aatcctctgc cctgaaaaaa
     5881 cagtgctgat gcctgatttg gaggcggaat gttctttgga tttgggctgt ccggaagaag
     5941 cgttttcagc gttttgcgac caacaccccg accgcacggt cgtagtgtac gccaacactt
     6001 ccgccgccgt gaaagcgcgt gccgactggg tggtaacgtc ttcggtggcg ttggaaatcg
     6061 tatcgtattt gaaatcgcgc ggcgagaagc tgatttgggg acccgaccgc cacctcggcg
     6121 actacatccg ccgcgaaacg ggcgcggata tgctgttgtg gcagggttcg tgcatcgtcc
     6181 acaacgaatt caaagggcag gagctggcgg cgttgaagac ggaacacccc gacgcggtgg
     6241 tgctggttca tcccgaatcg ccgcaaagcg tcatcgagct gggcgacgtg gtcggttcga
     6301 ccagcaaact actgaaagcc gccgtatcgc gtcccgaaaa gaaatttata gtggcaaccg
     6361 atctgggcat cctgcacgaa atgcaaaagc aggcgcccga caaagaattc atcgccgcgc
     6421 cgacggcagg caacggcgga agctgcaaaa gctgcgcgtt ctgcccgtgg atggcgatga
     6481 attcgctggg cggcatcaaa tacgccctga caagcggacg caacgaaatc ctgttggaca
     6541 gaaagctggg cgaagccgcc aaactgcctt tgcagcgtat gctcgacttc gcggcagggc
     6601 tgaagaaaaa agatgtgttc aacggcatgg gccccgcctg atttgccgtc catattgccg
     6661 tttcagacaa cctctcaaac aaggacaaca ccatgcaaac ggattgcgac gtattgattg
     6721 ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct gcctgaatcg ttccgcatcg
     6781 tcattttgtg taaaaaccgg ctggacgaca ccgccagccg tcatgcgcaa ggcgggattg
     6841 cggcggcgtg gtcgggagag gacgacatcg aaaaacacgt tgccgatacc ttggaagcgg
     6901 gcgcgggttt gtgcgacgaa gccgccgtcc gcaccatcct gtcgcagggc aaaccggcaa
     6961 tcgaatggct gctggcgcag ggcgtggcgt tcgaccaaaa taataacggc ctgcacctga
     7021 cacgcgaagg cgggcatacc tgccgccgaa tcgcccacgt cgccgattac acgggcgaag
     7081 ccgtcatgca gagcctgatt gcccaaatac gccgccgccc gaacatccgc gtttgcgagc
     7141 ggcagatggc gttggacatc caaaccgaat caggcgcggc gtgcgggctg accgtcctca
     7201 accgccaaac gcaagaaacc taccgcatcc gcgcccgcca taccgtactc gcaggcggtg
     7261 gtttgggaca gatttacgcc gccaccacca cgccgcccga atgcacgggc gacgccatcg
     7321 ccatggcaat acgcgcaggc tgcgcagtcg aaaacctcga atttatccaa ttccacccca
     7381 caggcttggc aaggccgtct gaaaacggcc gcaccttcct gatttccgaa gccgtgcgcg
     7441 gcgaaggcgg catcctgacc aaccaagcgg gcgaacggtt tatgccgcat tacgaccgcc
     7501 gcgccgaact cgcaccgcgc gacatcgttg cccgcgccat tgccgccgaa accgccaaac
     7561 aaacgcaaga cttcgtctcg ctcgacatca gccatcaacc cgcagcgttc gtccgccagc
     7621 attttccgtc catccatcga cactgtttat cccaatgcgg cctggatatc acgcgccaag
     7681 ccatccccgt ccgccccgtg caacactaca cctgcggcgg catccaaacc gaccccagcg
     7741 gcagaacttc cctgccgcag ctctacgcct taggcgaaac cgcctgcaca gggttgcacg
     7801 gagccaaccg cctcgccagc aactccctac tcgaatgcgt cgttaccgcc aggcttgccg
     7861 cccaaaccat cgcggatgga caagcgttcc aaaccgtacc gtccaaaagg tcgtctgaaa
     7921 acacctccgc cgaagcagac atcttttcag acggcatcca aaacacattc agccgcccca
     7981 ccctgcaagc gttcaaccaa cgccatctgg gcatcctccg caacgatacc ggcctgcgcc
     8041 gcgccatcgc ccaactgcgg ctttggaagc aaaaccaaac cgagccgcac actgcgtccg
     8101 aatacgaaaa ccgcaacctg ctcgaatgca gcctcgccgt cgcccaagcc gcatacgcaa
     8161 ggcggcaaaa catcggcgcg cattttaata gtgattgtta aggttggcag gtcagagaaa
     8221 atcgttcaaa taaatttgaa tagcatggtt aagaaattgg cattcggttt ttagggttgc
     8281 gtaagttggg ttatcaatct gttctaaaag cagaaaggcc gagacctttg caaaattccc
     8341 caaaatcccc taaattccca ccaagccatt taggggattt tccatgagca ccttcttccg
     8401 gcaaaccaca caagccatga ttgccaaaca catcgaccgt ttcccgctat tgaagttgga
     8461 tcaggtaatt gattggcagc cgatcgagca gtacctgaac cgtcaaaaaa accgttacct
     8521 ccgagaccac cgcggccgtc ctgcctatcc cctgctgtcc atgttcaaag ccgtcctgct
     8581 cggacaatgg cacagcctct ccgatcccga actcgaacac agcctcatta cccgcatcga
     8641 tttcaacctg ttttgccgtt ttgacgaact gagcatcccc gattacagca ccttatgccg
     8701 ctaccgcaac cggctggcgc aagacaatac cctgtctgaa ctgttggaac tgattaaccg
     8761 ccaactg
//
LOCUS       DAWFKN010000079         8734 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFKN010000079 DAWFKN010000000
VERSION     DAWFKN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.079"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            132..1301
                     /gene="prpF"
                     /locus_tag="WGR46_001749"
     CDS             132..1301
                     /gene="prpF"
                     /locus_tag="WGR46_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0856635.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1715..2914
                     /locus_tag="WGR46_001750"
     CDS             1715..2914
                     /locus_tag="WGR46_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0856636.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            3272..3892
                     /locus_tag="WGR46_001751"
     CDS             3272..3892
                     /locus_tag="WGR46_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0856637.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(4019..4462)
                     /locus_tag="WGR46_001752"
     CDS             complement(4019..4462)
                     /locus_tag="WGR46_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0856638.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4636..5004
                     /locus_tag="WGR46_001753"
     CDS             4636..5004
                     /locus_tag="WGR46_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0856639.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5049..6203
                     /locus_tag="WGR46_001754"
     CDS             5049..6203
                     /locus_tag="WGR46_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0856640.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6260..7132)
                     /locus_tag="WGR46_001755"
     CDS             complement(6260..7132)
                     /locus_tag="WGR46_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0856641.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7217..8029
                     /locus_tag="WGR46_001756"
     CDS             7217..8029
                     /locus_tag="WGR46_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0856642.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1902 a         2376 c         2519 g         1937 t
ORIGIN      
        1 cacaacacag tagccgtaga ttgtggcgaa ccccgacagt ttgcggaatc aaacggcttt
       61 ggtcggagtg gcagcctaat ccactataaa aatcgtgggc agagcccacg ctacataagg
      121 agaatctaga aatgccgcaa attaaaatcc ccgccgttta ctaccgcggt ggcacatcaa
      181 aaggcgtgtt tttcaaacgt tccgatctgc ctgaggcggc gcgggaagcg ggtagcgcgc
      241 gcgacaaaat cctgttgcgc gtactcggta gccccgaccc ttatggtaaa cagattgacg
      301 gtttgggcaa cgccagttcg tccaccagca aagccgtgat tttggacaag tccgaacgcg
      361 ccgatcacga tgtcgattac cttttcgggc aagtttccat cgacaaacct tttgtcgatt
      421 ggagtggcaa ctgcggcaac ctcaccgctg ccgtgggcgc attctccatc gagcaaggct
      481 tggtcgataa aggcaagatt ccttcagacg gcatctgcac ggtcaaaatc tggcagaaaa
      541 acatcggcaa aaccattatt gcccatgtac cgatgcaaaa cggcgcagtt ttggaaacag
      601 gcgattttga gctcgacggc gtaacgttcc cggcagccga agtacaaatc gaatttcttg
      661 atccagccga cggcgaaggc agtatgttcc caaccggcaa tttggtcgat gaaattgatg
      721 tgccgaatat aggccgtttg aaagccacgc tcatcaacgc gggcattccg accgttttcc
      781 tgaatgccgc cgacttgggc tacacgggca aagagttgca agacgacatc aacaacgatg
      841 ccgcagcttt ggaaaaattc gagaaaatcc gcgcttacgg tgcgctgaaa atgggtctga
      901 tcagcgacgt atccgaagct gccgcccgcg cgcacacgcc gaaagtcgcc ttcgtcgcgc
      961 ccgccgccga ttacaccgcc tccagtggca aaaccgtgaa tgccgccgac atcgatttgc
     1021 tggtacgcgc cctgagcatg ggcaaattgc accacgcgat gatgggtacc gcctctgttg
     1081 ccattgcgac cgccgccgcc gtgcccggta cgctggtcaa ccttgccgca ggcggcggaa
     1141 cgcgtaaaga agtgcgcttc gggcatcctt ccggcacatt gcgcgtcggt gcagccgccg
     1201 aatgtcagga cggacaatgg acggccacca aagcggttat gagccgcagc gcacgcgtga
     1261 tgatggaagg ttgggtcagg gtgccggaag attgttttta aattgacgta gcatgggttt
     1321 gcccgcgagc cataaacagg tcgtctgaaa aacaagtaaa catcaaatca ctgaccattc
     1381 ctttcccttg ccctgtggcg gaaggcggca aatcacaagg aagaacacgg aaaccccgat
     1441 aaaagacagc ttcccgtatt accgtcattc ccgcgcaggc gggaatctag acctgtcaat
     1501 atggaggatt ggtaggggaa aacaggtttc gtgagttcta cattctggat tcccgccaca
     1561 gcctgtcctc gcgtaggcgg ggacggaata acgatagaaa atgcggcata cgctttgccc
     1621 aaagaggccg tctgaaacac cttgcgcctg atgtctgcct ttttcagacg accccacacc
     1681 aaaaaaacaa ccacaaacta caaggagaaa catcatgtcc gaccaactca tcctcgttct
     1741 gaactgcggc agttcatcgc tcaaaggcgc cgttatcgac cgaaaaagcg gcagcgtcgt
     1801 cctaagctgc ctcggcgaac gcctgaccac gcccgaagcc gtcattacgt tcaacaaaga
     1861 cggcaacaaa cgccaagttc ccctgagcgg ccgaaattgc cacgccggcg cggtgggtat
     1921 gcttttgaac gaactggaaa aacacggtct gcacgaccgc atcaaagcca tcggccaccg
     1981 catcgcccac ggcggcgaaa aatacagcga gtctgttttg atcgaccagg ccgtaatgga
     2041 cgaactcaat gcctgcattc cgcttgcgcc gctgcacaac cccgccaaca tcagcggcat
     2101 ccttgccgca caggaacatt tccccggtct gcccaatgtc ggcgtgatgg atacttcgtt
     2161 ccaccaaacc atgccggagc gtgcctacac ttatgccgtg ccgcgcgagt tgcgtaaaaa
     2221 atacgctttc cgccgctacg gtttccacgg caccagtatg cgttacgttg cccctgaagc
     2281 cgcacgcatc ttgggcaaac ctctggaaga catccgcatg attattgccc acttaggcaa
     2341 cggcgcatcc attaccgcca tcaaaaacgg caaatccgtc gataccagta tgggtttcac
     2401 gccgatcgaa ggtttggtaa tgggtacacg ttgcggcgac atcgatccgg gcgtatacag
     2461 ctatctgact tcccacgccg ggatggatgt tgcccaagtg gatgaaatgc tgaacaaaaa
     2521 atcaggtttg ctcggtattt ccgaactttc caacgactgc cgcaccctcg aaatcgccgc
     2581 cgacgaaggc cacgaaggcg cgcgcctcgc cctcgaagtc atgacctacc gcctcgccaa
     2641 atacatcgct tcgatggctg tggcctgcgg cggcgttgac gcactcgtgt tcaccggcgg
     2701 tattggcgaa aactcacgta atatccgagc taaaaccgtt tcctatcttg atttcttggg
     2761 tctgcacatc gacactaaag ccaatatgga caaacgctac ggcaattcgg gtattatcag
     2821 cccgaccgat tcttctccgg ctgttttggt tgtcccgacc aatgaagaac tgatgattgc
     2881 ctgcgacact gccgaacttg tcggcatctt gtagccaaaa aagggacgag tccgcacaat
     2941 gccgtctgaa accccaaacg cccgattagg ctgatgagga ttttagacgg cattgttcat
     3001 ttttttgtta tcttgcattt ttgtgcggac ggtggaattt catcctgtaa acataaatat
     3061 ttgtcggaaa acagaaaccc tccgccgcca tttctacgaa agcaggaaac cagcaacgca
     3121 aagcgacagg gatttgttgg aaatgaccga aaccgaacga accggattcc cgcctgcgcg
     3181 ggaatgacgg gattttctgt ttttgtggaa atgacgggat tttgaatttc gggcgtacaa
     3241 tacggaaaac atgacgataa ggaaaaaaac tatggcacag tttttcgcta ttcatcccga
     3301 caacccccaa gaacgcctca tcaagcaggc ggtcgaaatc gtcaataaag gcggcgtggt
     3361 cgtttatccg accgattcct gttatgcctt gggttgcaaa ctcggcgata aggcggcgat
     3421 ggaacgcata ctctccatcc gaaaaatcga tttgaaacac cacctgaccc tgatgtgcgc
     3481 cgatttgagc gagttgggca catacgccaa aatcgacaac gtacagtttc gtcagcttaa
     3541 ggccgccacg cccgggcctt atacctttat cttgcaggcg acgaaggaag tgccggcgcg
     3601 cacgctgcac ccgaaacgca aaaccatcgg gctgcgtatt cccgataatg ccattgcaca
     3661 agccctgctg ggggaattgg gcgagccgct tttaagctgc accctgatgc tgcccgaaga
     3721 cggcgaacca ttgaccgatc cttatgaaat ccgcgagcgt ttggaacacg ccgtcgattt
     3781 ggtgattgac ggcggctggt gcggaaccga gccgaccacc gtcgtcgata tgaccgacgg
     3841 cacggaactg gtgcgccggg gttgtggcga tacggcggtg ttcggtttgt agggaaaccg
     3901 atgccgtctg aagcatcggc tgttcagacg gcattgcgcg ccttgccggc ggcagtccga
     3961 aatgccggcg cgtatcgcgc tcggtcggaa tatccgtttg aaacggcatt ttgatgcatt
     4021 actgcaccgc aatcggaatt ctcggttcgt agagcaggtc gtaggtcggc ttgttgagca
     4081 ggtcttggag cgtgaaaccg tccagatacg tgaaaaacga cttcattgca ccgccgagta
     4141 tgcctgtcag acggcaggac ggtgtaatca ggcattcgtt gttcgaaccc atacattcga
     4201 ccagctgcat cggttcgagg tggcggacga ccgagccgat gttgatgcgg tcgggcggtg
     4261 tggcaagccg cagaccgccg ccttttccgc gcacgctgtg gaggaagccg cctttgacca
     4321 gcgcggtaac gaccttcatc agatggcttt tggaaatgcc gtaggttacg gcgatggtac
     4381 tgatgttgac cagcgcatcg tcgttgatgg cagtgtagat aaggacgcgc agcccgtagt
     4441 ccgtatgttg tgtcaaatac atgattttct cggtatggat tgttattctt atcggtacgg
     4501 tttaaggttc acggacaata ccttaatggt tgaaaccctg tccgtcgggg cggtagaatg
     4561 cagcctgtct gcggcggtat gccgtctgaa acatccgcgc taccgtttga gaatttgtta
     4621 ttgtaactca aaatcatgaa accgttgaaa cgacatcccg cccttatcgg gctttcgcgt
     4681 gaccaccacc attcgctttc cctgtgcgtg cgtctgttgc ggacgccgga agaaaggcat
     4741 cgggacgaac tcgaaccgca tttttccgaa ttggaaaccc attttcgcga agaagaaacc
     4801 aagtttgccc caatttggca gaatgtcgcc cccgaattga aacaacgttt cgagaaagac
     4861 cacgcccgac tgcggcagat gatggcaagc cccgaatacg gtaacgcggc gtggaatacc
     4921 gcttttgcca caaccctgcg cgaccacgcg cgctttgaag aacgcgagct gtttcccgcc
     4981 gccgaaccgt ttttgccggc atgattccgt tttgcggcaa atatattaat gataaacaag
     5041 gaacacacat gaaatttacc aagcaccccg tctgggcaat ggcgttccgc ccgttttatt
     5101 cgctggcggc tctgtacggc gcattgtccg tattgctgtg gggtttcggc tacacgggaa
     5161 cgcacgagct gtccggtttc tattggcacg cgcatgagat gatttggggt tatgccggac
     5221 tggtcgtcgt cgccttcctg ctgaccgccg tcgccacttg gacggggcag ccgcccacgc
     5281 ggggcggcgt tctggtcggc ttgactatct tttggctggc tgcgcggatt gccgccttta
     5341 tcccgggttg gggtgcgtcg gcaagcggca tactcggtac gctgtttttc tggtacggcg
     5401 cggtgtgcat ggctttgccc gttatccgtt cacagaatca acgcaactat gttgccgtgt
     5461 tcgcgctgtt cgtcttgggc ggcacgcatg cggcgttcca cgtccagctg cacaacggca
     5521 acctaggcgg actcttgagc ggattgcagt cgggcttggt gatggtgtcg ggttttatcg
     5581 gtctgattgg tacgcggatt atttcgtttt ttacgtccaa acgcttgaat gtgccgcaga
     5641 ttcccagtcc gaaatgggtg gcgcaggctt cgctgtggct gcccatgctg actgccatgc
     5701 tgatggcgca cggtgtgttg gcttggctgt ctgccgtttt tgcctttgcg gcaggtgtga
     5761 tttttaccgt gcaggtgtac cgctggtggt ataaacccgt gttgaaagag ccgatgctgt
     5821 ggattctgtt tgccggctat ctgtttaccg gattggggct gattgcggtc ggcgcgtctt
     5881 atttcaaacc cgctttcctc aatctgggtg tgcatctgat cggggtcggc ggtatcggcg
     5941 tgctgacttt gggcatgatg gcgcgtaccg cgcttggtca tacgggcaat ccgatttatc
     6001 cgccgcccaa agccgttccc gttgcgtttt ggctgatgat ggcggcaacc gccgtccgta
     6061 tggttgccgt attttcttcc ggcactgcct acacgcacag catccgcacc tcttcggttt
     6121 tgtttgcact cgcgcttttg gtgtatgcgt ggaagtatat tccttggctg attcgtccgc
     6181 gttcggacgg caggcccggt tgagacaaac cgccgcagat ttcgggtctg ggcggtttgc
     6241 ttttcagacg gcagggcggt cagttgccgt ccagccagcg gtcgcgtgtg gttttggctt
     6301 cttcaaaata gcggtacagg gcttcgcggt cgtcggtggt caggatgttt gccaaaacgt
     6361 ccaactgttt gcccaagcct tgaaccagtt gcagcaggct gtctttgttg gcaaggcaga
     6421 tgtccgccca cacggcggga tgaccggagg cgatgcgggt gaagtcccga aagcccgtgg
     6481 cggcgaattt cagatattcc tgtccgtcgg ggtggtcgag aatctggtgg acataggcga
     6541 aggcggtcag gtggggcata tgggagacgg cggcgaaaac cgcgtcgtgg cgttgcgcgt
     6601 ccatcgtata aatttccgca ccgaccgcgt gccacaggtt ttctaccaag gcaatgccgt
     6661 ctgaatgttc gccgccgtgt ggcgtgatga tgagttttct gtggcggaac agcccgaact
     6721 gcgcggcttg cgcaccgctt ctgtccgaac cggcaatcgg gtgggcggcg atgcagcggt
     6781 gcaggcggtc gggcagacag cggcggaagg cttcgatgac cgaagatttg gtgctgccga
     6841 catcggaaat ccaagtgtgt tccggcaaaa cggggcgcag cgcggtcaaa atggcaggaa
     6901 cggtggcgac gggtgtggca atcagtacca agtccgcacc gccgatgctg tccgcgtcga
     6961 tggcaacgga agcccggtca atcacgccgc gttccaatgc acgttcgagg ttgtcgcggt
     7021 cggtgtcgat accggtaacg gtgcggacga gtccctgcct tttgaggtcg agaacgaacg
     7081 aaccgccgat cagccctaca ccgatgaggg caatatggtt caaaatgggc atttgtgtaa
     7141 acggttttcg caaagtaccg tcatggtagc ctatcggcgg aatatgccgc aaggtcggca
     7201 ggaaaaagga gaagaaatgg acaaaatcag agttgccgcc gtgcagatgg tgtcgggcgt
     7261 gtcgccggaa accaacgtcg ccgccatgaa acgcctggtc gcacgggcgg cggagcaggg
     7321 tgcggattgg gtgctgctgc ccgaatattg ggtgctgatg ggcgcaaacg ataccgacaa
     7381 actcgcgctt gccgagcctt tgggcggcgg acgctttcag acggcattga gcgaaacggc
     7441 gaaagaatgc ggcgtggtgc tgttcggcgg aaccgtgccg ctgcaaagcc ccgaggcggg
     7501 caaggtcatg aatactttat tggtgtacgg gcgcgacggc aaacggacgg ggctgtacca
     7561 caaaatgcac ctcttcggct tttccggttt gggcgaacgc tatgccgaag ccgataccat
     7621 cagcgcaggg cgggaagtgc cgcacttgtc ggcagaaggc gtgtcggtgg cggcgggcat
     7681 ttgttacgat gtccgctttc ccgaattttt ccgccgccag ctgccgtttg acgtattgat
     7741 gctgcccgct gcgtttacgc acacgacggg caaggcgcat tgggagctgc tgctgcgcgc
     7801 gcgtgccgtc gaaaaccaat gttacgtcgt ggcggcggca cagggcggtt tgcacgaaaa
     7861 cggacggcgc acgttcggac acagcatgat tgtcgatccg tggggcgacg tattggacgt
     7921 attacccgaa ggcgaaggca tcgttacggc agacatcgat gccaaccgcc tgaacagcgt
     7981 ccgcaaccgc ctgcccgcct tgaaataccg ggttttggat gccgtctgaa ggttcagacg
     8041 gcatcggtgc cggggaatca gcgggctgta ggatgggctt cagcctgcca tttccatcaa
     8101 tcccacattt ctaccgtttt catcgaatcc atcgaatccg cccctttcga cgacccagtc
     8161 ctacgcaact gaaccgtcat tcccacggaa gtgggaatct agaatctcgg gggtttcagt
     8221 catttccgat agattcccgc cgcgtcaggg gtctggattc ccgcctgcgc gggaatgacg
     8281 aatccatcca tacggaaacc tgcaccacgt cattcccacg aaagtgggaa tctagaacgc
     8341 ggggtttgag caactgtttt tatccgataa gtttctgtgc ggacaggtct agattcccgc
     8401 ctgcgcggga atgacgggtt ttaagattgc ggtattgtca ggaatgacga atccatccat
     8461 acggaaacct gcaccacgtc attcccacgg aagtgggaat ctagaatctc ggggtttcag
     8521 tcatttccga tagattcccg ccgcgtcggg ggtctggatt cccgcctgcg cgggaatgac
     8581 gaatttcgag attacggtgt tgtcggaacg aaactgaatc gtcattccca cgaaagtggg
     8641 aatctagaac gcggggtttg ggcaactgtt tttatccgat aagtttctgt gcggacaggt
     8701 ctggattccc gcctgcgcgg gaatggcggg tttc
//
LOCUS       DAWFKN010000080         8565 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFKN010000080 DAWFKN010000000
VERSION     DAWFKN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.080"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(80..598)
                     /locus_tag="WGR46_001757"
     CDS             complement(80..598)
                     /locus_tag="WGR46_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0856643.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(618..2003)
                     /locus_tag="WGR46_001758"
     CDS             complement(618..2003)
                     /locus_tag="WGR46_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0856644.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKFVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2242..3783
                     /locus_tag="WGR46_001759"
     CDS             2242..3783
                     /locus_tag="WGR46_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0856645.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3812..4411
                     /locus_tag="WGR46_001760"
     CDS             3812..4411
                     /locus_tag="WGR46_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0856646.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4408..5013
                     /gene="nadD"
                     /locus_tag="WGR46_001761"
     CDS             4408..5013
                     /gene="nadD"
                     /locus_tag="WGR46_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0856647.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5072..5458
                     /gene="rsfS"
                     /locus_tag="WGR46_001762"
     CDS             5072..5458
                     /gene="rsfS"
                     /locus_tag="WGR46_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0856648.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5512..5982
                     /gene="rlmH"
                     /locus_tag="WGR46_001763"
     CDS             5512..5982
                     /gene="rlmH"
                     /locus_tag="WGR46_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0856649.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6055..6321
                     /locus_tag="WGR46_001764"
     CDS             6055..6321
                     /locus_tag="WGR46_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0856650.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6478..7161
                     /locus_tag="WGR46_001765"
     CDS             6478..7161
                     /locus_tag="WGR46_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0856651.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7285..7797
                     /gene="coaD"
                     /locus_tag="WGR46_001766"
     CDS             7285..7797
                     /gene="coaD"
                     /locus_tag="WGR46_001766"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0856652.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7829..8482
                     /locus_tag="WGR46_001767"
     CDS             7829..8482
                     /locus_tag="WGR46_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0856653.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1862 a         2279 c         2502 g         1922 t
ORIGIN      
        1 atcaatgtaa tccattctgt tcccgatgtt tatgccgtct gaaacccatc ctgtgtcggg
       61 cttcagacgg catattgctt caaagcaggt tttccgaggc aacccagttc agaatatcgg
      121 cttcgaccgc ttcgggcgtg ccggggttgg cgattttgac cccgtattcg ccttcgccca
      181 gttcctcgag gatttccagt tgcgaatcga gcatccctgc tttcatgtaa tgtcctttgc
      241 gcgacatcat gcgctcgagg ttgatgtctt gcggcggact gaggtggatg aaggcagctt
      301 tgccttcggc tccgcgcaga atgtcgcggt agccgcgttt gagggcggaa caggttacga
      361 tggtgtggtt cgcaccgttt tgcgcctgtt gcgtcatcca gtcgcgcaga ttgcccaacc
      421 acggatagcg gtcttcatcg gtcagcggaa tacccgcgcc catcttgtcg cggttggctt
      481 gggtgtggaa ctcgtcgcct tcggcatagg gacattgacc gaggtgtttc tgcagggaca
      541 gcgcggcggt ggtcttgccg cagccgcata cgcccataac gacaaaatgc gtagtcattt
      601 actatccttt ccgtctgtca gacgatggcg aacagcagtg cggacaaggc aaagccgatg
      661 agtgcgatga gggtttggtt gaccgtccag gttttcagcg tggtcggtac gtccatgtcc
      721 aagagacggc cgaccagcca gaagccggag tcgttgaagt ggctgcaacc gaccgaacct
      781 gccgccgttg ccaatacgat acaggcgagc tgccagtcgg taaagccggc ggcggcaacg
      841 gcaggagcca tcagcgcggc ggcggtggtc agggcgacgg ttgccgaacc ttgcgcgata
      901 cgcagtgcca aggcgacaag gaaacagccc aaaaggacgg gaatgcccaa atccgccatg
      961 ctgtcggcga gtgccttgcc gatgccggaa gcgcgcaaaa cgccgccgaa cataccgccc
     1021 gcgccggtaa tcagaatcac ggaacagacg ggggcgagtg cgccgtccac ggttttttcc
     1081 aacgcgctgc cgctttcgcc gcgtttgcgt cccaagacaa acagtgcgac caatacggaa
     1141 atcagaaggg cgatcggtgt cgaaccgatt atttttgccg tctgaaccca ggtttcgtcc
     1201 gcacttacga atttttcgct gatgagggcc gatacgccgg tattcaggaa aatcagcagc
     1261 atgggaatca gcatgatggc gacgaccgtt cctgctttgg caggttcttt cggcaggtcg
     1321 ttgtcttgcg tgccgccgct gagcagttcg ggaacgggaa catggatggt gcgccccaac
     1381 actttgccga gcatatagcc gctgaaatac catgtgatga aggcggtcgg cagacccaaa
     1441 atcaaaactt ggccgatgtt cgcgccgtaa aattcggaag cggcaatcgg gcccggatgg
     1501 ggcggcagga agacgtgcat gacggaaaat gcgccgatgg aggcaagcgc gaagggcagt
     1561 acgtcctgtt tcatgcgccg tgcggtggcg aacacgatgg gcagcatgac gattagtccg
     1621 gcatcgaaga aaatcgggaa gccgaaaatc agcgaggcaa cgcccagcgc gaacggtgcg
     1681 cgtttttcgc cgaacatccg gatcagcgcg tccgccagcg actgtgcgcc gccggatgtt
     1741 tcgaccagac gtccgagcat cgcgcccagg ccgaccagaa gcgccacgcc gccgagcgtg
     1801 ccgccgaagt ttttgaccag tacgtcgttg acaatgctgc ctgtgggcaa accggttgcc
     1861 aaagccgtca gcaggctgac gatgaccagt gtcagcagcg cgtggatgcg gaatttgacg
     1921 attaaaatca gaatgaggat gattgccgcc gccgaaatgc ccaacagggt ttgcgcggac
     1981 agcgtctgtg tccagccgtc catttgtaaa gcctttctct gtaataaaag tacaaaattg
     2041 tcaggttttt ttaagttgtg cgaaaacgat agcacaaatc gggcggtaag ttgtttgtct
     2101 gaagttatat tcctgtttat ttgaacgatt tatttcagac ggcgggagga aggcggatac
     2161 ggtatgtagt cggattgaaa tatacggtat gtggggcttt tgggtacaat cgcaacatca
     2221 gtgtttcaag tggggaaggc gatggatgga cggcgttggg cggtatgggg tgcttttgcc
     2281 ctgctgcctt cggctttttt ggcggtaatg gtcgttgcgc ctttgtgggc ggtggcggcg
     2341 tatgacggtt tggcgtggcg cgcggtgctg tcggatgcct atatgctcaa acgtttggcg
     2401 tggacggtat ttcaggcagc ggcaacctgt gtgctggtgc tgcctttggg cgtgcctgtc
     2461 gcgtgggtgc tggcgcggct ggcgtttccg gggcgggctt tggtgctgcg cctgctgatg
     2521 ctgccttttg tgatgcccac gttggtggcg ggcgtgggcg tgctggctct gttcggggcg
     2581 gacggcctgt tgtggcgcgg acggcaggat acgccgtatc tgttgttgta cggcaatgtg
     2641 tttttcaacc ttcctgtgtt ggtcagggcg gcgtatcagg ggtttgtgca agtgcctgcg
     2701 gcacggcttc agacggcacg gacgttgggc gcgggggcgt ggcggcggtt tttggacatt
     2761 gaaatgcccg ttttgcgccc gtggcttgcc ggcggcgtgt gccttgtctt cctgtattgt
     2821 ttttcggggt tcgggctggc attgctgctg ggcggcagcc gttatgccac ggtcgaagtg
     2881 gaaatttacc agttggtcat gttcgaactc gatatggcgg ttgcttcggt gctggtgtgg
     2941 ctggtgttgg gggtaacggc ggcggcaggg ttgctgtatg cgtggttcgg caggcgcgcg
     3001 gtttcggata aggcggtttc ccctgtgatg ccgtcgccgc cgcagtcggt cggggaatat
     3061 gtgctgctgg cgtttgcggc ggcggtgttg tctgtgtgct gcctgtttcc tttgttggca
     3121 attgttgtga aagcgtggtc ggccggcgaa tcgtggcgtg tgttaatgga aagtgaaacg
     3181 tggcaggcgg tgtggaatac tttgcgcttc tcggcggcgg cggtgtatgc ggcggcggtt
     3241 ttgggtgtgg tgtatgcggc ggcggcgcgg cggtcggcgt ggatgcgcgg gctgatgttt
     3301 ttgccgttta tggtgtcgcc ggtttgtgtt tcggcgggcg tgctgctgct ttatccgcag
     3361 tggacggctt cgttgccgct gctgctggcg atgtatgcgc tgctggcgta tccgtttgtg
     3421 gcaaaagatg ttttatcagc ctgggatgca ctgccgccgg attacggcag ggcggcggcg
     3481 ggtttgggtg caaacggctt tcagacggca tgccgcatca cgttccccct cttgaaaccg
     3541 gcgttgcggc gcggtctgac tttggcggcg gcaacctgcg tgggcgaatt tgcggcaacc
     3601 ttgttcctgt cgcgtcccga gtggcagacg ctgacgactt tgatttatgc ctatttggga
     3661 cgcgcgggtg aggataatta cgcgcgggcg atggtgctga cattgctgtt ggcggcgttc
     3721 gcggtgtgca ttttcctgct gttggacggc ggcgaaggcg gaaaacggac ggaaacgtta
     3781 taatgcgaac cctttttcag aggacggcaa aatgagcgag ttggataaca tccttgccca
     3841 caaccggcag tttgtcgaat cgggcgaata tgaaaaatac tttaccgaca aataccccga
     3901 acgcgggctg gcggttttgt cctgtatgga tgcgcggatt atcgggctgc tgcccgacgc
     3961 gttgggtttg aaaaacggcg atgccaagct gattaaaaat gccggcgcgc tggttacgca
     4021 cccgtggggt tcggtgatgc ggagcctttt ggttgccgtg tttgaattga aggtcagaga
     4081 gattatggtc atcgcccatc acgattgcgg tatgcagggg ctgaatgccg aagaattcct
     4141 cgggcgcgtc cgggaaagcc ggattcccga agaccgtatc gaaaccctgc gttatgccgg
     4201 tatcgacctc gacggctggc tgaccggttt cgacaacgtc gaagacagcg tgcgccacac
     4261 ggtggacctt atccgtaacc atccgctgat gccgcgccat attgccgttc acggattggt
     4321 catccatccc gttaccggca aactgacgct ggttgtggac ggcagtgttt cagacggcat
     4381 ggacttatcg gaaggaatgg aaacatcatg aagaaaatcg gactgttcgg cggcactttc
     4441 gacccgatac acaacgggca tctccacatc gcccgcgcct ttgccgacga aatcggcttg
     4501 gacgcggttg ttttcctgcc gacaggcggc ccgtatcaca aagacgcagc ctccgcttcc
     4561 gccgccgacc gccttgccat ggtcgaactg gcgacggcag aagacgcgcg ttttgccgtc
     4621 agcgattgcg acatcgtccg agaaggtgca acctatactt ttgataccgt ccaaatcttc
     4681 cgccagcagt tcccatccgc gcaactctgg tggctgatgg gcagcgacag cctgatgaag
     4741 ctgcacacat ggaaaaaatg gcagatgctc gtgcgcgaaa ccaatatcgc cgtcgccatg
     4801 cggcagggcg acagcctgca ccaaaccccg cgcgaactgc acgcgtggct gggcaagtcc
     4861 cttcaggacg gcagcgtccg catcttgtcc gccccgatgc ataatgtgtc gtcaacggaa
     4921 atccgccgca accttgccgg ccaaggcgtt tcagacggca tcccgcctgc cgccgcacgc
     4981 tacatccgcg aacacggttt gtatgaaaaa taaagtcaaa tcagtaagaa acggctataa
     5041 tgccgtctga aaacatcccg ttaggaacat aatgaacgaa caagaactgc aagacctgca
     5101 aaaaatggtc ggggtcgccg tcaacgccct cgaagacatc aaagccaaag acatttccgt
     5161 tctcgaaacg caagacaaaa cttcgctgtt tgccagaatg attatcgcca gcggcgacag
     5221 tacgcgccaa gtcaaagcac tggccaacaa cgttgccgtc gatttgaaag aagccggttt
     5281 tgaaatcctc agtaccgaag gagacagcgg cgaatggacg ctggttgacg caggcgacct
     5341 cgttgtccac gtcatgctcc ctgccgtgcg cgacttctac gacatcgaca ccctttgggg
     5401 cggcgagaaa ccgagtttcc acgccggaat gcagaagccg tggcacgcgg cagactgatt
     5461 cccgatgccg tctgaacctt cagacggcat tttcctgtca gggagaaagc attgaacatt
     5521 accgttttgg cagtcggcac caaaatgccg cgctgggttg acgaagccgt cgccgaatac
     5581 gccaaacgct tcggacgcga cgtcgcctac gcactcaaag aaatcaaacc cgaaaaacgc
     5641 ggcgcgggcg tgaatgccgc ccaaggtatg gcggcggaag aaaaacgcat ccttgaagcc
     5701 attccgcaag gcgcgttcct cgtcgttctt gacgaacgcg gcaaagcacc gacctccgtc
     5761 gagctggcgg aacacctcaa aagctggcag caaaacggcg aacacgtctg cttcgtcatc
     5821 ggcggcgcgg acggaatgac cgaccgcctc aaacaacaag cccgtataat gatgcgcctg
     5881 tccagcctga ccctgccgca cgggatggtg cgcgtccttc tgaccgagca gctctaccgc
     5941 gccgcctcca tcctgcacaa ccatccctat catcgggaat aagaaggctt ttgtccgtat
     6001 ccctaatcgg ttaaaattac cccgttattt ctgaacacag caaaggaatc cgctatggca
     6061 cgcatggtat tctgcgtcaa gctcaacaaa gaagccgaag gcatgaaatt tccaccgctt
     6121 cccaacgaat tgggcaagcg catttttgaa aacgtatcgc aagaagcgtg ggcggcgtgg
     6181 acgcgccacc aaacgatgct gattaacgaa aaccgtttaa gcctcgccga tccgcgcgcg
     6241 cgcgaatacc tggctcagca gatggagcag tatttcttcg gcgacggcgc ggatgccgtt
     6301 cagggatacg ttccgcaata acggttttcc gttttgaaca caggctgtcc gaaactgctt
     6361 cagacggcct ttaaaatacg ccggacaact ttatcttcac gcaaccggac aaattttgac
     6421 attgggcaat gttaaaatcc cccattattc ccaaccgcgc tttcaggagc agatgatatg
     6481 caacacgacg tttacgacta caccgcgcat acggtttcta aaaacaccgt cctgcagaaa
     6541 acctaccgcc tgctcggatt ttcattcatt ccggctgccg caggcgcggc acttgccgcc
     6601 aatgccggtt tcaattttta cgccgccttc ggttcgcgct ggatagggtt tgccgtcgtg
     6661 ttggcgtttt tctacggtat gatccacttc atcgagaaaa accgttacag caataccggc
     6721 gttaccctgc tgatggtatt cacattcggt atgggcgtat tgatcggccc cgtgctgcaa
     6781 tacgcgctcc atattgccga cggtgcgaaa atcgtcggca ttgccgccgc gatgaccgcc
     6841 gccgtctttt taacgatgtc cgccttggcg cgccgaaccc ggctcgatat gaacgcgctc
     6901 ggacgcttcc tgaccgtagg tgcggtcatt ctgatggtcg ccgtggttgc caatctgttt
     6961 ttgggtattc ccgcactcgc cctgaccatt tccgccggtt ttgtcttgtt cagttcctta
     7021 atgattatgt ggcaggtacg caccgtcatc gacggcggcg aagacagcca catcagcgcg
     7081 gcactgacac tgtttatctc gctttacaac atcttcagca gcctgctgaa catcctgttg
     7141 tctttaaacg gcgaggattg atgtcggtaa aacgccgtcc gactatgccg tctgaaaatg
     7201 cttcagacgg cattttactt tgggcataca atacggatgc acacgcacca acacacagcc
     7261 gactatgaag ccgggaggaa tcaaatgtta ccgaatacac cgcgccgcgc cgtttatgcc
     7321 ggcagtttcg atccgcccac attggggcat ctgtggatga tacggcaggc gcaatctatg
     7381 tttgacgaac tcatcgtcgc catcggcatt aaccccgaca aacgcagcac ctataccgtc
     7441 gctgaaaggc aggatatgtt gtgcgatatt actaaaatgt ttcccaacgt cagaaccgat
     7501 gtatttgaaa accgatttct ggtgcattat gcccgtgagg tagatgcagg attcatcgtg
     7561 cgcggcatcc gttctgcttc ggattacgaa tacgaacgtt ccatgcgcca catcaacagc
     7621 gacctcgccc ccgaaatatc caccgtattc ctcatgccgc cgcgcgaaat cgccgaagtg
     7681 tcgtccacta tggtcaaagg actggtcggg ccggaaggct ggacggaaac gatccaccgc
     7741 tacgtgccgc aagctgtgta cgaaaaaatc cttgccgaac atcaacacga aaattgaacc
     7801 cttctatttt tgcaaggcat atatggatat gttggaaatt ttgttccgcc atcaagactt
     7861 cgtcgccatc aacaaaccgg gcggcatatc cgtccaccaa gacagcggcg agaccggact
     7921 ggcccgaaca ctcgccatac agttgggcgt agagcgtgtg tggctgctgc accgattgga
     7981 caaacagacc agcggcattt tactgtttgc cctcaacaaa gaaagcgcgt ccgcccttag
     8041 cggacagttt gccggcaaaa gcatcaaaaa aacctatttg gcactgtccg accgcaagcc
     8101 gtccaaaaag cagggttgga ttaaaggcgg aatggaaaaa tccagatgtg gaatgtggaa
     8161 gttgacgcgc aatacagaaa atatcgccgt tacccgattc cacagcatca gtatcgccga
     8221 aaaactgcgg ctgttcatcc tggaaccgca tacgggcaaa acgcaccaat tgagagtggc
     8281 gatgaaaagt ttgggcagtc cgattctggg cgacagctta tacggcggaa cagaatccga
     8341 aaccatgttt ctgtatgcat ggaaaataca atttgcctac caaaaccgac aaatcgaaat
     8401 tgttgcaccc ttaaaaaacg aatggcagac tgaaaatatc tctcatgcgc tggaagaatt
     8461 ttatatggca aaagcaggtt aataaatata aattaattta taaacaatat gataaatatt
     8521 tttataaaaa tttttcaaga aaacggttga ctgcaaatct cttca
//
LOCUS       DAWFKN010000081         8343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFKN010000081 DAWFKN010000000
VERSION     DAWFKN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.081"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(116..1219)
                     /locus_tag="WGR46_001768"
     CDS             complement(116..1219)
                     /locus_tag="WGR46_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0856654.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGR46_001769"
     CDS             complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGR46_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0856655.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPNEPRQAAGSASRPELEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDMDLARTALQDIIAEKHKVITADLIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3303..3437
                     /gene="rpmH"
                     /locus_tag="WGR46_001770"
     CDS             3303..3437
                     /gene="rpmH"
                     /locus_tag="WGR46_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0856656.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3440..3805
                     /locus_tag="WGR46_001771"
     CDS             3440..3805
                     /locus_tag="WGR46_001771"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0856657.1"
                     /translation="MDYRFGKQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRPTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3870..4091
                     /gene="yidD"
                     /locus_tag="WGR46_001772"
     CDS             3870..4091
                     /gene="yidD"
                     /locus_tag="WGR46_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0856658.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4263..5900
                     /gene="yidC"
                     /locus_tag="WGR46_001773"
     CDS             4263..5900
                     /gene="yidC"
                     /locus_tag="WGR46_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0856659.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5981..6694)
                     /locus_tag="WGR46_001774"
     CDS             complement(5981..6694)
                     /locus_tag="WGR46_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0856660.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCVAADLTLPTQEIISRTIAQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGR46_001775"
     CDS             complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGR46_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0856661.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7491..7994
                     /locus_tag="WGR46_001776"
     CDS             7491..7994
                     /locus_tag="WGR46_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0856662.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8028..8207
                     /gene="rpmF"
                     /locus_tag="WGR46_001777"
     CDS             8028..8207
                     /gene="rpmF"
                     /locus_tag="WGR46_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0856663.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         2057 a         2482 c         1989 g         1815 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatac ctttccgaca gccgaacaaa accccgaatc
       61 cgtctgcacg gttcggggca tatctccaat acgggcatcg tgctccggaa aaccgtcaaa
      121 ttcgcatcgg catcacgata tatttgaaat tcggattgtt cggcacggta aacagcgtcg
      181 agcggttggc atcgccgaag gcaagctgca tatcgtcgga atggatgttg cgcaacacgt
      241 ccatcagata gccgatattg aaaccgactt cgagttcgcc gccctgatag gcaatctcga
      301 tttcttcgcg cgcttcttcc tgctcgttgt tgctgcacac gacgctcaac aggccggatt
      361 gcaggaacag gcgcgcgcca cggaattttt cgttggcaag aatggacaca cgttccagcg
      421 cgcccaaaag ttcggcacgg gacaaaacga aaatcttgtc gttgtccaaa ggaatcacgc
      481 ggttgaaatc ggggaatttg ccgtcgatga ccttgctgac gatggtcgtg ccgttgcatt
      541 ggaaacgcac ctgtttgtcc agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca
      601 gtttgaacag ttccagcacc gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg
      661 catcaatcgc gcaggctgca taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact
      721 ggctgccctc aacctgcatc agcagaccgt tgagataata gcggatgtcc tgcaccgcca
      781 tgctgtactg cacttgcgac agcatggttt tgaaacgctc ctgctccagc gagaaagtcg
      841 cgctgatgtc ctcgccgaca ttcatcatcg gaaaatcggc ggcaggcagg gtctgcaggg
      901 caaaacgcga tttgcccgcc ttcagcgtca gacggctgtc gtcccaatcc agcgacacca
      961 gcgcaccggc aggcagtgcc cgtaaaatgt cctgaaattt cttggcattg gtggtgatgc
     1021 ggaagtcgtc cgcgccgccc tcgggacccg cagtgtcgat ttggatttcc aaatcggttg
     1081 ccaagagttt ggtctgaccg ccttttccct caatcaggac gttggacagg atgggcaggg
     1141 tgtggcggcg ttcgacgatg ccggtaacgg cttgcagcgg cttgagtagg ctgtcgcgtt
     1201 cggcttgtaa aatcaacatt ttcgctcctt tgaatcggtt tgtatagtgg attaaattta
     1261 aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacgaaaccg attcacttgg
     1321 tacttcagca ccttagagaa tcgttctctg tgagctaagg cgaggcaacg ctgtactggt
     1381 ttaaatttaa tccgctatat ctttaccctt cggacggcat gggcaatatc atgtcgtctg
     1441 aaaacgtttt ccatcagttt tgaatcagaa tcagcagttt ctcgtaatcc tgcgccaact
     1501 cggggtcttc ctcgcgcagt ttcgccaccg ccctgatgcc gtgcatgacg gtcgtatggt
     1561 cgcgtccgcc gaacgaatcg ccgatagacg gcaggctcaa agtggtcagt tctttggtca
     1621 ggctcatggc aacctgacgc ggacgggcaa tgttgcgcgt gcgttttttg ccgagtacgt
     1681 cgctgatttt gatgcggtaa tatttcgcca ccgcatcaat gatgaggtcg gcggtaatga
     1741 ctttgtgctt ctcggcgata atgtcctgca acgccgtacg cgccaaatcc atatcgatga
     1801 cggggcggtt cataaagcgg ctgctcgcgc tgacgcggtt gaatgcgcct tcaagctcgc
     1861 gcacattgga acggatcagg ttggcaataa acaatgccgc ctcgtcttcg atgctgatac
     1921 ccgccgcctc tgcctttttc tgcaaaatgg cgatgcgcat ttccaattcg ggcggctcga
     1981 gttccaaagt caaaccccaa gagaaacgcg atttgagccg gtcgtccatg ccttcgattt
     2041 tggcgggtaa aacatcgcaa gtgaggatga gctgtttttt ctcgttgtgg aaatggttgt
     2101 acagatagaa aaactcttcc atcgtacggt ctttgccttt gatgaactgg atatcgtcga
     2161 taatcagcag gtcgtattgc ttgtattgct gcttgaacac gtcgtaggta ttgttgcgaa
     2221 ccgccttcat aaagctgcgg atgtagtcgt ccgaatgcat atagcgcact ttcgcatcgg
     2281 gacggttttt caaaagctcg ttgccgaccg cctgcacaag gtgggttttg cccaaacccg
     2341 tgctgccata gaggaagaac gggttataac tctgtcccgg actttccgct atcgcctgcg
     2401 cggcggcagc tgcaaggcgg ttgcccttac cttcgaccaa cgtatcaaac gtgtaatccg
     2461 gagacaggtt ggtttgttcg taacgcgcct cttccgcatc acgctgcgca tccgtccgcg
     2521 ctttcgcaag cacctccaat tccggacggg aagcagaccc ggcagcctga cgcggctcgt
     2581 tcggcaggtt tttcatacgt tccgccaaaa tatccgccgc cgttttcgac gcagcgggtt
     2641 tgacaggctc ttcagacggc agctcgtcca acagaacctc ctgcacaggc atctcctccg
     2701 accccgcgtg caaggacggc tcggcaggtt cgacagcacc ttcaaccgcc gccatctcat
     2761 aacgcacgcc ttctcccggt ttgaatacga aggcggaacg gccggcagcc aactcttccc
     2821 tcaccgcttc tatttttccg gcaaactggc tcttgagcat attgcaggca aactggttct
     2881 tgccgtacac cacccatacg ccaccctcct caccgacggt aaggggcgca atccattgcg
     2941 caaactgccc gtgaggcaac atatcgtgaa gacggcggag gcacagcggc caaaactctg
     3001 ctaatgtcat ggataggctc gaatcggtaa aaatgaaatc gaaaacaaag aaaatataat
     3061 attttcaaaa agaaaacaaa tctgttgaac acacatcggt tcaaaatgca gcgaaccgat
     3121 tataccgact cgccgatatt ttatccacaa cccgtgcaaa aatttatcca cagaaaggcg
     3181 gcagaaatcc gcaggcaatc gggcaatcct cctgcaaagt ttctatattg attgacaaaa
     3241 gcggcaaatt ggagtgtaat tcacggttta attatctacc cattctattt ttaggaaaca
     3301 tcatgaaacg cacttatcaa ccttccgtta ccaaacgcaa acgcacccac ggcttcctgg
     3361 tgcgctccaa aacacgcggc ggccgcgcag tactggcagc acgccgtgcc aaaggccgca
     3421 aacgcctggc agtgtaattt tggactaccg cttcggaaag cagtaccgct tgttaaaaac
     3481 ggatgatttt tcatccgttt ttgcattcag aaaccgccgc agccgcgacc tgctgcaagt
     3541 ttcgcgctca aacggcaacg ggctgggcca tccccgcatc ggtctggtga tcggcaaaaa
     3601 aaccgccaaa cgcgccaacg aacgaaatta tatgaagcgc gtcatccgcg actggttcag
     3661 attgaacaaa aaccggctgc cgccgcagga tttcgtcgtg cgcgtccacc gtaaattcga
     3721 caggcctacc gcaaaacagg caagggcgga actggcacaa ctcatgttcg gcaaccccgc
     3781 aaccggatgc aggaaacagg catgatcaga acggtactct gcaggcaagg ttcagacggc
     3841 aacgggtttc ccatacaagg aacatcccga tgaacttcct attgtccaaa ctcctgctgg
     3901 gactgatacg gttctaccaa tattgcatca gcccgctgat tccgccgcgc tgccgttata
     3961 cgccgacctg ttcgcaatac gcggtcgaag cggtcaaaaa atacggcgca ttcaaaggcg
     4021 gtcggctcgc catcaagcgc attgcacgct gccacccttt cggcggacac ggacacgacc
     4081 ccgttccctg acccgacgca atattcaaat tgcacgcttt ccttttattt cccatcggtt
     4141 tctatataat gccgtctgaa acttcgggct ggcggcacga ccgtcgggta tgaagcccac
     4201 ccttattccc cgtctatcgg aacacgcaac ctgcggcatt tccgaccatt caggaaactc
     4261 ttatggattt taaaagactc acggcgtttt tcgccatcgc actggtgatt atgatcggat
     4321 gggaaaagat gttccccact ccgaagcccg tccccgcgcc ccaacaggca gcacaacaac
     4381 aggccgtaac cgcttccgcc gaagccgcgc tcgcgcccgc aacgccgatt accgtaacga
     4441 ccgacacggt tcaagccgtc attgatgaaa aaagcggcga cctgcgccgg ctgaccctgc
     4501 tcaaatacaa agcaaccggc gacgaaaata aaccgttcat cctgtttggc gacggcaaag
     4561 aatacaccta cgtcgcccaa tccgaacttt tggacgcgca gggcaacaac attctaaaag
     4621 gcatcggctt tagcgcaccg aaaaaacagt acagcttgga aggcgacaaa gttgaagtcc
     4681 gcctgagcgc gcctgaaaca cgcggtctga aaatcgacaa agtttatact ttcaccaaag
     4741 gcagctatct ggtcaacgtc cgcttcgaca tcgccaacgg cagcggtcaa accgccaacc
     4801 tgagcgcgga ctaccgcatc gtccgcgacc acagcgaacc cgagggtcaa ggttacttta
     4861 cccactctta cgtcggccct gttgtttata cccctgaagg caacttccaa aaagtcagct
     4921 tctccgactt ggacgacgat gccaaatccg gcaaatccga ggccgaatac atccgcaaaa
     4981 ccccgaccgg ctggctcggc atgattgaac accacttcat gtccacctgg atcctccaac
     5041 ccaaaggcgg acaaagcgtt tgcgccgctg gcgaatgcag catcgacatc aaacgccgca
     5101 acgacaagct gtacagcgca agcgtcagcg ttccccttgc cgccatcccg gccggcgcga
     5161 aagccgaaac cgccgtcaac ctgtatgccg gcccgcaaac cacttccgtc atcgcaaaca
     5221 tcgccgacaa cctgcaactg gccaaagact acggcaaagt acactggttc gcctcccccc
     5281 tcttttggct tttgaaccaa ctgcacaaca tcatcggaaa ctggggctgg gcaatcgtcg
     5341 ttttgaccat catcgtcaaa gccgtcctct acccgctgac caacgcgtcc taccgctcga
     5401 tggcaaaaat gcgcgccgcc gcacccaaac tgcaagccat caaagagaaa tacggcgacg
     5461 accgtatggc gcaacaacaa gcgatgatgc agctttacac agacgagaaa atcaacccgc
     5521 tgggcggctg cctgcctatg ctgttgcaaa tccccgtctt catcggattg tattgggcat
     5581 tgttcgcctc cgtagaattg cgccaggcac cttggctggg ttggattacc gacctcagcc
     5641 gcgccgaccc ctactacatc ctgcccatca ttatggcggc aacgatgttc gcccaaacct
     5701 atctgaaccc gccgccgacc gacccgatgc aggcgaaaat gatgaaaatc atgccgttgg
     5761 ttttctccgt catgttcttc ttcttccctg ccggtctggt attgtactgg gtagtcaaca
     5821 acctcctgac catcgcccag caatggcaca tcaaccgcag catcgaaaaa caacgcaccc
     5881 aaggcgaagt cgtttcctaa atgccgcagc atgaaaaatg ccgtctgaaa cctgttcaga
     5941 cggcattttt attgttcacc ccctatcggg gcggaaatct tcaacctgca tacatcacaa
     6001 aaatcgtcgg gcgttttttc agattgggca tttctttcct tttccgccac tgcgcgattg
     6061 tccggctgat gatttcctgt gtcggcaagg tcaaatccgc agctacacac aagcgcgttt
     6121 cagaatgcag gttttccacc gcatccgcca acaacacatc gttgcgatac ggcgtttcga
     6181 taaacagctg cgtttcattc tgctggcgcg aacgttgctc caaagccttc aaactctgaa
     6241 tccgctcgtt tttttcagac ggcagataac ccttaaacgc aaaactctgc ccgttcgcac
     6301 ccgaagccat caaagccagc agcaggctgg aaggcccgac cagcggacgc acttcaaaac
     6361 cgtgtttatg cgccaaagcc accagattcg cacccggatc ggcaacagcc gggcaacccg
     6421 cctcgctgac aatgcccata ctgcgccctt cttgcaaagg tttcagcaat tccagcaaag
     6481 tcttcaaatc cgtgtgttcg ttcagcgttt gcaaattcag ttcgcggata ggcattgtca
     6541 cgcccaaatg tttcaaatgc gcgcgcgccg ttttttccgc ttccacgaca aaatctgtca
     6601 gcccgacaat cgcctgttgt tcatgcggca acaggcacgg cgtgtcaggc gtacccaaag
     6661 gcgtaggaat caaatacaaa acaggagaca ccattccctc actcatcagt taaaaatgcc
     6721 gtctgagcct ttcagacggc ataaacgggc agttacagaa cctccacgcc ctcatttttc
     6781 aagaaatcga ccagacggaa aaccggcaaa ccgattaaag cattcggatc gctgctttca
     6841 atccgttcaa tcagcagcgc gcccaagccc tcgctcttca acgcacacga gcaataaacc
     6901 gcatcaggct cgcgctccaa atagcggagg atgtgcagct cgtccaactt cctcatcacg
     6961 accaccgtct tatcgatatg ccgttgcatt ctgcccgtaa ccgtatttag cagaacgacc
     7021 gcgctgtaaa actcaatctc cctgccgctc aaatgcatca acatcttttg cgcattagca
     7081 aggttcatcg gcttgcccca ctgcctgccg tcgcaccacg ccacctggtc cgcaccgaca
     7141 atcaacgcct ctgggaaacg cccggtcaac gaccgcgcct taccctcggc aaggcgcaat
     7201 gccgtctgag gggcggattc ccccaacatc ggcgtttcgt caaaatcggg ggacgccgcc
     7261 tgaaaggcaa tgccgagcct ttccatctgt tcgcggcgga aaaccgaact cgtacccaaa
     7321 atcaaaggca gttccaaacc catcccatcc tccttaccgt tgaaaacacg cccgaagggg
     7381 cagtaaaatc cagccatgcg ccgaaacacg gatacccgcc ttcggcgtac cgcaacattt
     7441 ttcttaaaaa tattgacgtt agaacatcta aattatatca tatcccgttt atgtcagacc
     7501 ctaatttgat tgacccggaa atttttgccg ccgaagggca gaacctgcaa ggcagttttc
     7561 tgctggaaga attggatgaa cgcgtcagtt cgcacgatta tcccgccgac aggcagacca
     7621 aaatatcgtt tacactgacc ggcggtcgcg accggctgca acgcctgttc ctcgacctga
     7681 acgtcaaagc cgatatgccc ctgatttgcc agagatgtat caaacccatg ccgttcatgc
     7741 ttgatgaaag cagccgtatc gtcctgtttt ccaacgaaga gtccttggac gaatccatgc
     7801 ttgccgacga agaactcgaa ggcatactga ttgaaaaaga actcgacgtg cgcacattgg
     7861 tagaagacca aatcctgatg tccctgccct tttcgccgcg acacgaagac tgcggcgaca
     7921 atgggacact ggaagaagtc aatcgggaca aacccaaccc ctttgctgtt ttggcaggtt
     7981 tgaaaagcaa ttgattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac
     8041 aaaacaaaaa atccccttcc aaacgcggta tgcaccgttc gcacgacgcg ctgaccgcgc
     8101 ctgcactgtc tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca
     8161 acggtatgta ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact
     8221 gactgaaaaa gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca
     8281 tccgtccaaa ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataataaccc
     8341 gtg
//
LOCUS       DAWFKN010000082         8283 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFKN010000082 DAWFKN010000000
VERSION     DAWFKN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.082"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR46_001778"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR46_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0856664.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WGR46_001779"
     CDS             complement(1659..2042)
                     /locus_tag="WGR46_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0856665.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WGR46_001780"
     CDS             complement(2046..3587)
                     /locus_tag="WGR46_001780"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0856666.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WGR46_001781"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WGR46_001781"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0856667.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4774..5997
                     /locus_tag="WGR46_001782"
     CDS             4774..5997
                     /locus_tag="WGR46_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0856668.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     STSLLWFSAVIGPILGVTAVFFQRTAQKFPFIKRDNIKIILLAVCMFALVGAVAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6158..7738
                     /gene="purH"
                     /locus_tag="WGR46_001783"
     CDS             6158..7738
                     /gene="purH"
                     /locus_tag="WGR46_001783"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0856669.1"
                     /translation="MSVIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIATAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1837 a         2255 c         2356 g         1835 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacatcac gctgttttcg ttgaagaaca gtgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 cacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcggggtt
      421 gacggtgtcg ttcgcaccga tgaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaaaacga tgtcgtaggg gactttggct tcggcgagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcgg tgccgttttt
      601 acgtaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacat tgtttgcgcc tttcagcatt tcggcaatat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat aatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 catgaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctacc gttaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gtttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc tcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 tttttaatgc cgtctgaact gttcagacgg catctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcccgcccac atcagcgagc gcaatgctgc tttgtctgcg
     1741 atgtagcacc agataaaggc gaggcggaac aggatgaaca ggccggcaag cgtgttgacg
     1801 gttgcttgtc cggcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgcgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataagcag gcaatcagga tgcaccaata ggcaaaagtc
     2041 atgctttatc ctttcctaaa catattcaac atgcggcgcg ttacgaagaa gccgccgaag
     2101 atattgatgc tggcaatcag gatggcgatg aaggcaagca gggtaacgaa accgttgcct
     2161 tggctgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggttaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggctgcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg cgccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtgatt
     3121 tggccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccatg
     3241 tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ctcgccttcg
     3361 gacggcgcat tgaccttata aatcaaaggg caggcccaaa ccgccgcttt gtcggcaacg
     3421 gtcgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg gggcagggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tttccgattg cagtcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagtt accgccatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga acctgattac
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cgaggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcgac
     4081 gaactcggtt taattgtcag cgatatggat tcgacgctga ttaccattga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaagtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 ttcttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcgggttc
     4381 acatttttta ccgaaaggct gcaacaacga ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggt cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc gcatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agcgcgggcc gccgccgatg cctgtatcaa cttcggcggt
     4681 ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgcc gtctgaaagg
     4741 ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacaac atacggcata cggttatggc acggacggcg tgtccatctc gttccgcgaa
     4921 ggtgtggcac aggcttccgg tatgcggcgc gttgccgtgc tgacgctgtg cggcgcggtc
     4981 gcaggcagcg gctggtggtt gctgaaacgt ttcggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaaac agccgttgca ggggctgccg tttctgacga cggttttcca tgttctgctg
     5101 caaatcataa cggtcggact cggttcaccg ctcggacggg aagtcgcccc gcgcgaaatg
     5161 accgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggacgaagg cgaaatgcgg
     5221 ctgctgattg cttgcgcttc gggtgcgggt ttggcggccg tgtataacgt gccgctcgcc
     5281 tccacgctgt tcatcctcga agccatgctg ggcgtgtgga cgcagcaagc cgtcgccgcc
     5341 gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca tcggcttagg cgacgtgcag
     5401 caatatcatc ccgccaacct tgccgtcagc acttcattac tttggttttc cgccgtcatc
     5461 ggtccgatac tgggcgtaac cgccgtcttt ttccaacgta ccgcccaaaa attccccttt
     5521 atcaagcgcg acaatatcaa aatcattctc ttggccgtct gtatgtttgc actcgtcggc
     5581 gcggttgccg tttggtttcc cgaagttttg ggcaacggca aagcaggcaa ccaactgacc
     5641 tttggcggct tgaccgactg gcaacacagc cttgagctga ccgccgtcaa atggctggtc
     5701 gtattgatgg cacttgccgc cggtgcatac ggcggcctga ttacgccgtc gatgatgctc
     5761 ggcagcacca tttcttttgc tgccgccgcc gcgtggaaca gcgtcttccc cgaaatgccg
     5821 tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtatcgct caaaatgccg
     5881 ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct gctgctgcct
     5941 ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     6001 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg tcaacaatat
     6061 ctgcaaaaac ttactgccaa aaaactgaaa atagcggata ataccgcccc gaaaaagttc
     6121 ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     6181 gatcagcctg tccgacaaga cgggcgcagt cgaatttgcc caaaccctgc acaagctcgg
     6241 tgtcgaaatc ctctctaccg gcggcacggc gaagctgctt gccgatgcgg gcgttcccgt
     6301 gattgaagtt gccgactaca ccggtttccc cgaaatgctc gacggccgcg tgaaaacgct
     6361 gcatccgaaa atccacggcg gtattttggg ccggcgcgat ttgcctgaac acgtcgccaa
     6421 gatggaagaa cacggcatcg gcaacatcga cctcgtgtgc gtcaacctct accccttcgc
     6481 tgccaccatc gccaaaccaa actgcacgct ggaagacgcg attgaaaaca tcgacatcgg
     6541 cggcccgacc atggtgcgct ctgccgcgaa aaactggaaa cacgtcgcca tcgttactga
     6601 cacagccgat ttcccggcca tagctgccga actcgaagcc aacaacggcg cattgagcga
     6661 caaaacccgt ttcaacctgt cgcgcaaagc gttcagccac accgcccaat acgacggcat
     6721 gatttccaac tacctgacct cgctttcaga cgacgttttg agcggagagc ctgaaatcgg
     6781 cgaatttcca agtcggttca accaaagctg gattaaagtg caagacatgc gctacggcga
     6841 aaacccgcat cagcgcgccg cgttctaccg cgatattgac cccgccgcag gcagcctttc
     6901 cgcctacaaa caactgcaag gcaaagaatt gtcgtacaac aacatcgccg atgccgatgc
     6961 cgcatgggaa gccgtcaaat cctttgacgc gccagcctgt gtgattgtga aacacgccaa
     7021 tccgtgcggc gtagctgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     7081 cgacaccacc agcgcgttcg gcggcatcat cgctttcaac cgcgaagtgg acggcgaaac
     7141 cgtcaaacaa attaccgaca accagtttat ggaagtcctg atggcgccga agtttaccgc
     7201 cgaagccctc gaaattgcca ccgccaagaa aaacgtgcgc gtgttggaag tgcctttgga
     7261 agcaggcgcg aaccgcttcg aactcaaacg cgtcggcggc ggactgttgg tacaaacccc
     7321 cgacattcac cgcctcaacc gcgccgattt gaaagtcgtt tccaaacgcc aaccgaccga
     7381 gcaagaatgg aacgacctga tgtttgtttg gaacgtcgca aaatacgtca aatccaacgc
     7441 catcgtcttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     7501 ggacagtacc cgcatcgccg cccgcaaagc gcaggacgca ggcttggact taaacggcgc
     7561 atgtgccgcc tctgatgctt tcttcccctt ccgcgacggc gtggacgtga ttgccgagca
     7621 gggcatcaaa gccatcatcc atccggcagg ttcgatgcgc gatcaggaag tcttcgacgc
     7681 agcggacgaa cacggcatcg ccatggtcgt aaccgacgtg cgccacttcc gccattaacg
     7741 cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg ctattttgtg
     7801 aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt gttgtatagg
     7861 gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct gcatcaaaaa
     7921 tttttggaga atcggcggga gtcggcggtt ttggtttcgg cggggacggt ggaaatgggt
     7981 aacattgacg gaatcgacgg aagcggtggg cttcagccca ccgcttgtat attggaatca
     8041 ccgtatcata gcgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag
     8101 cggcagggca acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg
     8161 caggtttatg atgagtaccc aataccatta cgcaggtata gtgaattaaa tctaaaccag
     8221 tacagcgttg gttcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
     8281 tga
//
LOCUS       DAWFKN010000083         8279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFKN010000083 DAWFKN010000000
VERSION     DAWFKN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.083"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..456)
                     /locus_tag="WGR46_001784"
     CDS             complement(<1..456)
                     /locus_tag="WGR46_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0856670.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEVDESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTRTATLFPIIREQVKPDSIVYTDCYRSYDVLDVREFSHFSFAETSFS"
     gene            518..628
                     /locus_tag="WGR46_001785"
                     /pseudo
     CDS             518..628
                     /locus_tag="WGR46_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            747..1151
                     /locus_tag="WGR46_001786"
     CDS             747..1151
                     /locus_tag="WGR46_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0856671.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(1186..2061)
                     /gene="rsmI"
                     /locus_tag="WGR46_001787"
     CDS             complement(1186..2061)
                     /gene="rsmI"
                     /locus_tag="WGR46_001787"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0856672.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            2108..2455
                     /locus_tag="WGR46_001788"
     CDS             2108..2455
                     /locus_tag="WGR46_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0856673.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            2460..3053
                     /locus_tag="WGR46_001789"
     CDS             2460..3053
                     /locus_tag="WGR46_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0856674.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            3117..3725
                     /locus_tag="WGR46_001790"
     CDS             3117..3725
                     /locus_tag="WGR46_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0856675.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3733..4377
                     /locus_tag="WGR46_001791"
     CDS             3733..4377
                     /locus_tag="WGR46_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856676.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            4417..5196
                     /gene="map"
                     /locus_tag="WGR46_001792"
     CDS             4417..5196
                     /gene="map"
                     /locus_tag="WGR46_001792"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0856677.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            5351..5650
                     /locus_tag="WGR46_001793"
     CDS             5351..5650
                     /locus_tag="WGR46_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0856678.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5888..6262
                     /locus_tag="WGR46_001794"
     CDS             5888..6262
                     /locus_tag="WGR46_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0856679.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(6450..7916)
                     /locus_tag="WGR46_001795"
     CDS             complement(6450..7916)
                     /locus_tag="WGR46_001795"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0856680.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         2156 a         2441 c         1987 g         1695 t
ORIGIN      
        1 cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg cgcacatcta atacatcata
       61 gctacgataa caatccgtat aaacaatgct gtcaggtttc acttgttcac ggataatagg
      121 aaataaagta gcggttcgag tattcggtac tgtaaccgta taaaccttac catttcgctt
      181 caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc ctttgcgttg
      241 tccgccaaaa taactttcat ctacttctac ttcgccatca aacatttcca aatgcggact
      301 gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtat ttttattaac
      361 gcctactaac tctgctgccg ttcttgcagt tacacctgcg acaaacagtt caatgagttt
      421 attttgttta taccggctta gacgactttt tctcataggg ataattctaa cttaatttga
      481 atttccctag ttatctagga cagcccctaa gttttttatg aacacaaacc aacctgccgt
      541 ttacgacccg ttgacacgta cgctgcactg gctgaccgtt gccggtttca tcggcattcc
      601 gaccaccatt gtcctgtgga cgatttatag tagattaaca aaaatcagga caaggcgacg
      661 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gtttttgtta
      721 atccactata cgaagaggcg gaatgggtgg gcagcctgtt cggcctgcac aaatctttcg
      781 gtttccttac gctgacggtg attacattgc gcatcgtgtg ggcggttgcc aaccgcgcca
      841 agcgtccgca aagcgacagc aaggctgcgg cggcaggaca cggcattctg tatctgctca
      901 tgcttgccgt tcccgttatc ggcatgatcc gccaatacgg cagcggccgc ggcccgttga
      961 aagtgttcgg cgttgaagtg atgcagggtt cgccggaaaa aatcgagtgg atggcaaact
     1021 tgggcaacac gttccacggc aatttgggct ggctgctgtt tgccgccgtc gtcggacacg
     1081 tcgccatggt cgtcgtccac cgtgttcaag gcaaagacgt gctgtaccgc atgacggggc
     1141 gtgtccgttg attccgttcg cactatggtg ccggtccgtc cggcatcatt tgtttttcca
     1201 agacagtgcc agattgtaca aagctttttt tccctcgccc gtgattttgg cggcaagctc
     1261 cgccgcctgt ttggtcggca gctcggctgt gaggattttc atgatgtttt gcgcggactc
     1321 ggacaagcct tcgtgttttt catcctgcgc cggataaagc accaacacca tctcgccgcg
     1381 cgattggttg ccgtcggcag acaatgccgt ctgaatttcc ccaaccgtgc cgcttaagaa
     1441 cgtttcaaac gttttcgtaa tttcgcgcgc cagcattaat cggcgttcgg ggaacagttc
     1501 cgccatatcg gcaagcgtcg caccgatgcg gtgcggcgtt tcaaacatga cgataggaaa
     1561 cgccgcccgc acccatttgg caaacagttt cctgcgttct cccgatttcg gcggtacaaa
     1621 accgttgaaa taaaaatcgg atccttccac accggccacg ctcaaagccg ccatcaccgc
     1681 gcttgcgccc acgacgggaa cgactttaaa cccggcctca cgcacgcggc cggcgagttt
     1741 ggcacccggg tcgcacacgg ccggcgtacc cgcatcggaa acctgtgcca caaccatgcc
     1801 gtctgaaaga tagccgacaa tcttgtccgc catctgccgt tcgttgtgtt cgcgcacact
     1861 gacaagtttg ccctgaatgc cgtacgcgct caaaagctgc gcggtaacgc gcgtgtcttc
     1921 ggcacagatg atgtccgcct tttgcaatac cgccaaagcg cgcagggtaa tgtccgccaa
     1981 attgccgatg ggcgtggcaa ccacgtataa tgtccctccg acgacgctgt cggaggcttt
     2041 ctgcaagtgt ttctgaaaca taaaaaatgc cgtctgaaaa acagacatta taaaggttaa
     2101 accgattatg cgcctaaacc acaaacaggg cgaggcaggg gaagatgccg cgcttgcctt
     2161 cctccaatcc caaggctgca cactgcttgc ccgcaactgg cactgcgcct acggcgaaat
     2221 cgacctgatt gtcaaaaacg gcggcatgat tctgtttgtt gaagtaaaat accgcaaaaa
     2281 tcggcaattc ggcggtgtcg catacagcat ttccccatcc aaattattga aactgcaacg
     2341 aagtgtagag tattatctgc aacagaacag gttgacaaac gtaccgtgcc gcctcgatgc
     2401 ggtactgatt gaaggaaacc gcccgcccga gtggatacag aatattacag gttgacgata
     2461 tgacgacatt acaagaacgc gttgccgccc attttgccga aagcatccgt gccaagcagg
     2521 aagccggaaa agtattggtc gagccgaccg tacaggctgc cgagctgatg ctgcaatgcc
     2581 tgatgaatga cggcaaaatc ctggcctgcg gcaacggcgg ttcggctgcc gacgcgcaac
     2641 acttcgccgc cgaaatgacc ggccgttttg aaaaagaacg catggaactc gccgctgtcg
     2701 cgctgacaac agacacttcc gcgctgacag ccatcggcaa cgactacggt ttcgaccacg
     2761 tattcagcaa acaggtacgc gccctcggac gtgcaggcga cgtcttggtc ggcatttcca
     2821 cttccggcaa ttccgccaac gtcatcgaag ccgtcaaagc cgcacacgaa cgcgatatgc
     2881 acgtcatcgc cctgaccggc cgcgacggcg gcaaaatcgc cgccatgctc aaagacaccg
     2941 acgttttact caacgttccc tatcctcgaa ctgcccgtat tcaggaaaac cacatcctgc
     3001 tgatacacgc catgtgcgac tgtatcgact ccgtactgct ggaaggaatg taaccctttt
     3061 cagacggcat ggcgcaaagc aatgccgtct gaaacgccca agaaaggaag cccccgatga
     3121 aacccaaacc gcacaccgtc cgcaccctga ttgccgccat tttcagcctt gcccttagcg
     3181 gctgcgtcag cgcagtaatc ggaagcgccg ccgtcggcgc gaaatccgcc gtcgaccgcc
     3241 gaaccaccgg cgcgcaaacc gacgacaacg ttatggcgtt gcgtatcgaa accaccgccc
     3301 gttcctacct gcaccaaaac aaccaaacca aaggctacac gccccaaatc tccgtcgtcg
     3361 gctacaaccg ccacctgctg ctgctcggac aagtcgccac cgaaggcgaa aaacagttcg
     3421 tcggtcagat tgcacgttcc gaacaggccg ccgaaggcgt gtacaactac attaccgtcg
     3481 cctccctgcc gcgcactgcc ggcgacatcg ccggcgacac ttggaacaca tccaaagtcc
     3541 gcgccacgct gttgggcatc agccccgcca cacaggcgcg cgtcaaaatc gttacctacg
     3601 gcaacgtaac ctacgttatg ggcatcctca cccccgaaga acaggcgcag attacccaaa
     3661 aagtcagcac caccgtcggc gtacaaaaag tcatcaccct ctaccaaaac tacgtccaac
     3721 gctgactcgg caatgccgtc tgaaccgcct tcagacggca ttgcccgaca ccccaaaagc
     3781 acaatcaaaa tggcaaaaaa accgaacaaa cccttcaggc tgacccccaa actcctgata
     3841 cgcgccgtat tgctcatctg tatcaccgcc atcggcgcat tggcaatagg catcgtcagc
     3901 acattcaacc cgaacggcga caaaaccctt caagccgaac cgcaacacac cgacagcccc
     3961 cgcgaaaccg aattctggct gccaaacggc gtagtcggac aagatgccgc ccaacccgaa
     4021 caccaccacg ccgcctcatc cgaacccgca cagccggacg gcacagacga aagcggcagc
     4081 ggactgccgt cccctgccgc acccaagaaa aaccgggtca aaccgcaacc tgccgacaca
     4141 gctcaaaccg acaggcagcc ggacgacgcc ggaacacaag ctgaaaacac actcaaagaa
     4201 acccccgtac tgcccacaaa cgtcccccgt cccgaacccc gaaaagaaac acccgaaaaa
     4261 caggcgcagc ccaaagaaac gcccaaagaa acgcccaaag aaaaccatac caaaccggac
     4321 accccgaaaa acacgccgcc caaaccccat aaagaaattc tcgacaacct cttctgaccc
     4381 ggcacggcag gcacacccgc aatccaagga agcattatga acggcatcat catcaaaacc
     4441 cccgaagaaa tcgaaaaaat gcgcgagctg ggcaaactcg tcgccgaagc cctcgactac
     4501 atcggacaat tcgtcaaacc cggcgtaacc accgacgaaa tcgacaaact cgtttacgac
     4561 taccacgtca acgtccaagg cggctatccc gcccccctgc actacggcaa cccgccctac
     4621 cccaaatcct gctgcacctc cgtcaaccac gtcatctgcc acggcattcc cgacgacaag
     4681 ccgctcaaag aaggcgacat tatcaacatc gacctcacca tcaaaaaaga cggcttccac
     4741 ggcgactcca gccgtatgtt taccgtcggc aaagtctccc ccatcgccca acgcctgatc
     4801 gacgtaaccc acgcctccat gatggcgggc atagaagccg tcaaacccgg cgcgacactg
     4861 ggcgacgtag gttacgcctg ccaacaggtt gccgaaaacg ccggctattc cgtcgtacag
     4921 gaattctgcg gacacggcat cgggcgcggt ttccacgaag ccccgcaagt gttgcactac
     4981 ggaaaaaaag gacagggctc cgttctaaaa ccgggtatga tttttaccgt cgaaccgatg
     5041 atcaaccaag gcaaacgcca cctgcgtatc ctcaacgacg gctggacggt ggttaccaaa
     5101 gaccgctccc tctccgccca atgggaacac gaagtcttgg tgaccgaaac cggctacgaa
     5161 atcctcaccg tcagccccgc ctccggcaaa ccctgaagcc ggacgtatcc gccccataaa
     5221 aacaaacaat gccgtctgaa agaaacggca gatatgatat ataatataaa aacaggcttg
     5281 acccggcaca ttacgaaaac aaagcaaatc ggaatttgcc ccgcaaccag acaaacttaa
     5341 aggaagtttt atgaaaatat ttgaaaatat agaagatgtt aaagccatcc gtaaaaagac
     5401 cgggctgaac cagatagact tctggggcaa ggtcggcgtt acccagtctg gaggctcgcg
     5461 ctacgaaacc ggccgcaaaa tgcccaaacc cgtacgcgaa ctgctccgcc tcgtccatat
     5521 cgaatgcatc gatttggcga aagtcaacaa aaaagatatg gaaatcgccg ccctgttgaa
     5581 aaaacaccat cccgacctgt atgccgagtt gtccaaacag accaagtccg aaagaaaaaa
     5641 acaaagttaa accgcaacct ccggatgccc gacagttttt catttccgaa aaacgcaaac
     5701 aatgccgtct gaaacaccgg acaggtcgcc gtatcccgcc tgccgcccct gcctcaaacc
     5761 gccgaaccgc ccgaacccgc ctttttacaa actttatcca atttcctgtt tatttcagga
     5821 tacgccgaca ttagaatgtc aaacagctcg aaacgggcaa actccacatc catcccaagg
     5881 aataaaaatg aaacttctga ccaccgcaat cctgtcttcc gcaatcgcgc tcagcagtat
     5941 ggctgctgct gccggcacga acaaccccac cgttgccaaa aaaaccgtca gctacgtctg
     6001 ccagcaaggt aaaaaagtca aagtaaccta cggcttcaac aaacagggtc tgaccacata
     6061 cgcttccgcc gtcatcaacg gcaaacgtgt gcaaatgcct gtcaatttgg ataaatccga
     6121 caatgtggaa acattctacg gcaaagaagg cggttatgtt ttgggtaccg gcgtgatgga
     6181 tggcaaatct taccgcaaac agcccattat gattaccgca cctgacaacc aaatcgtctt
     6241 caaagactgt tccccacgtt aatcaggcaa caaaaaacag cgttttcaga aatgaaaacg
     6301 ctgttttttt gaccgttcca ttattcgcaa aagggaaaaa acgattacct gccccgtgta
     6361 tcaaaacctg ccctgccgga tgaagggcat aaccggcagg gacggcatca acaccatatg
     6421 ggggtacggc ttttcttgaa agattcggct taaatatcca acactttcgc ggtataggcg
     6481 ataatttcat ccgccctttc ggggttttcg ttcaacttga tgccgtaacc cggtaccagc
     6541 tctttcagac ggccttccca agacggggcg cgctcgggga agcattggtg catcagccgg
     6601 atcatcagcg gcacagcagt cgatgcgccc ggcgacgcgc ccagcaatgc ggcgagtgag
     6661 ccgtcggcgt gggcgacaat ctccgtacca aactggagca cgccgccttt ttcggagtct
     6721 tttttaatga tttggacgcg ttgccctgcg gtgatgagtt cccagtcgtc ggggtttgcc
     6781 tcggggtagt attccagcag ggaggcgaag cgttcttctt tggttttacg caattcgccc
     6841 agcaggtatt tggtcagcgg catattcgcc cagccggcgc acagcatagg atagaggttg
     6901 tccatatgga tggacagcgg caaatccata agcgagcctt gcttgaggaa gttggaacgg
     6961 aagcctgcgt aagggccgaa cataaggtgg cgtttgccgt ccacgttgcg tgtgtcgagg
     7021 tgcgggacgg acatcggcgg cgcgccgacg gaagcctgcc cgtacacttt ggcgttgtgt
     7081 tgttcggcgg tttcaggggt gctgttgcgg aagaacaggc cggacacggg gaagccgccg
     7141 tagcctttgc cttcggggat gccggatttt tgcagcaggg tcagcgcgcc gccgcccgcg
     7201 ccgaggaaga gaaagcgggt acggagggtg agctgcccgt cggggttgcg ggtatcggcg
     7261 gttttgagca cccacgcgcc gtcggattcg cgtttgatgt cttcgacgtg gcggttgaac
     7321 tcggttttta cgcccttgcc ctgcaaatat ttcaccattt ggcgcgtcag ccgtccgaaa
     7381 tcgacatccg tgccttcggc ggagtagttg gcggcgacgg gttggttttc gtcccggccg
     7441 cgcatcatca gcggagccca atcggaaatt ttgttccgat cggtggaaaa ttccatattt
     7501 tcaaaaagtt tttgggtttt aaacgcgtca taacgttttt gaagataaga acaatggtct
     7561 tcattcatca ccaaagacat atgcggcacg gcattgatga aggaattgtc ttccaacttg
     7621 ccttccgcga ccaacgtcgc ccaaaactgg cggctgacat gaaactgttc ggcaatattg
     7681 agggcgcgcg ccggatcgat aatcccattt gcacccaacg gcgcatagtt caattcgcac
     7741 agcgcggaat gccccgtgcc ggcgttgttc cacgcgtttg acgattccaa cgccacatct
     7801 tccaagcgtt caatcagggt gatttcccaa gacggttcga gttctttgag caaaacgccc
     7861 aaagtcgcgc tcataatgcc gccgcccacc aagacaacgt ctgtcgcttc agccatggtt
     7921 tactcctaaa aaacaggcat cttctgccct tatggttatt tgccgtacta caaacgcctg
     7981 aatcgcaaaa gcagggaaaa ccggcaatgg tgtgtgtccg agtatgctgt ttcagggttg
     8041 gaatgcgttg caagtatggc ttccgacacc gcttcagggg cttgtaatat gttatcgtga
     8101 atgtagtgga ttttactggg aaatgcaaag tttttctgtc gcccgccaag cccgggaact
     8161 gcgaaatgaa aaataaaaat agttatttat ctatatatat caaattttta atagataaaa
     8221 aaccaaaatt gtttatatat taatttttaa aagattgtca gcatattgcg ttaagtttt
//
LOCUS       DAWFKN010000084         8151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFKN010000084 DAWFKN010000000
VERSION     DAWFKN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.084"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            1..2045
                     /locus_tag="WGR46_001796"
                     /pseudo
     CDS             1..2045
                     /locus_tag="WGR46_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2287..3753
                     /locus_tag="WGR46_001797"
     CDS             2287..3753
                     /locus_tag="WGR46_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0856681.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNSGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3954..4922)
                     /locus_tag="WGR46_001798"
     CDS             complement(3954..4922)
                     /locus_tag="WGR46_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856682.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5000..6292
                     /locus_tag="WGR46_001799"
     CDS             5000..6292
                     /locus_tag="WGR46_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0856683.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(6795..7481)
                     /locus_tag="WGR46_001800"
     CDS             complement(6795..7481)
                     /locus_tag="WGR46_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0856684.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7556..8029)
                     /gene="queF"
                     /locus_tag="WGR46_001801"
     CDS             complement(7556..8029)
                     /gene="queF"
                     /locus_tag="WGR46_001801"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0856685.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(8033..8077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         1934 a         2017 c         2446 g         1754 t
ORIGIN      
        1 gtcgggttaa tctattaaca ttatctgttt tatcgctgtt ttgcacgcca tatgtttgag
       61 gttcggatgc gtacgatccc gtcaaagaag ccgagattaa aaacaaattt attttagaag
      121 cggcggaaga cagaaattcc cacgtttggc gcggcccgtg cagcatatct tttgattgct
      181 tcggtatgtt cagagctcag cttggttcaa atactcgttc taccaaaatc ggcgacgatg
      241 ccgatttttc attttcagac aagccgaaac ccggcacttc ccattatttt tccagcggta
      301 aaaccgatca aaattcatcc gaatatgggt atgacgaaat caatatccaa ggtaaaaact
      361 acaatagcgg catactcgcc gtcgataata tgcccgttgt taagaaatat attacagata
      421 cttacgggga taatttaaag gatgcggtta agaagcaatt acaggattta tacaaaacaa
      481 gacccgaagc ttgggaagaa aataaaaaac ggactgagga ggcgtatata gaacagcttg
      541 gaccaaaatt tagtatactc aaacagaaaa accccgattt aattaataaa ttggtagaag
      601 attccgtact cactcctcat agtaatacat cacagactag tctcaacaac atcttcaata
      661 aaaaattaca cgtcaaaatc gaaaacaaat cccacgtcgc cggacaggtg ttggaactga
      721 ccaagatgac gctgaaagat tccctttggg aaccgcgccg ccattccgac atccatatgc
      781 tggaaacttc cgataatgcc cggatccgcc tgaacacgaa agatgaaaaa ctgaccgtcc
      841 ataaagcgta tcagggcggc gcggatttcc tgttcggcta cgacgtgcgg gagtcggacg
      901 aacccgccct gacctttgaa gacaaagtca gcggacaatc cggcgtggtt ttggaacgcc
      961 ggtcggaaaa tctgaaaacg ctcgacgggc gcaaactgat tgcggcggaa aaggcagacc
     1021 ctaattcgtt tgcgtttaaa caaaattacc ggcagggact gtacgaatta ttgctcaagc
     1081 aatgcgaagg cggattttgc ttgggcgtgc agcgtttggc tatccccgag gcggaagcgg
     1141 ttttatatgc ccaacaggct tatgcggcaa atactttgtt tgggctgcgt gccgccgaca
     1201 ggggagacga cgtgtatgcc gccgatccgt cccgtcaaaa attgtggctg cgcttcatcg
     1261 gcggccggtc gcatcaaaat atacggggcg gcgcggctgc ggacgggcgg cgcaaaggcg
     1321 tgcaaatcgg cggtgaggtg tttgtacggc aaaatgaagg cagccggctg gcaatcggcg
     1381 tgatgggcgg cagggccggc cagcacgcat cagtcaacgg caaaggcggt gcggcaggca
     1441 gttatttgca tggttatggc gggggtgttt atgctgcgtg gcatcagttg cgcgataaac
     1501 aaacgggtgc gtatttggac ggctggttgc aataccaacg tttcaaacac cgcatcaatg
     1561 atgaaaaccg tgcggaacgc tacaaaacca aaggttggac ggcttctgtc gaaggcggct
     1621 acaacacgct tgtggcggaa ggcgttgtcg gaaaaggcaa taatgtgcgg ttttacctgc
     1681 aaccgcaggc gcagtttacc tacttgggcg taaacggcgg ctttaccgac agcgagggga
     1741 cggcggtcgg actgctcggc agcggtcagt ggcaaagccg cgccggcatt cgggcaaaaa
     1801 cccgttttgc tttgcgtaac ggtgtcaatc ttcagccttt tgccgctttt aatgttttgc
     1861 acaggtcaaa atctttcggc gtggaaatgg acggcgaaaa acagacgctg gcaggcagga
     1921 cggcactcga agggcggttc ggcattgaag ccggttggaa ggattatttg tctgcgcgtc
     1981 tcggatacgg caaaagatcg gacggcgata aagaagccgc attgtcggtc aaatggctgt
     2041 tttgatgcgt cgggaaatgt tttgacgcac aggcggcaca ccggcacggc cccgcgcgcc
     2101 gccccgcaaa ccaatccgaa ccctgccgcc ccgaagggcg gggcatcatg atgaaaccgg
     2161 cggaaaaccg ccggtttttt gccgccgttt gaaacccgat tctggcttca gacggcattg
     2221 tcgcggcatc gggcggcagg gtttggaaca gcggcataaa aaactgatac aatccgccga
     2281 ttgataatgg ttatttttta tttttgtggg aagacattta tgcctgcacg aaacagatgg
     2341 atgctgctgc tgcctttatt ggcaagcgcg gcatatgccg aagaaacacc gcgcgaaccg
     2401 gatttgagaa gccgtcccga gttcaggctt catgaagcgg aggtcaaacc aatcgacagg
     2461 gagaaggtac cggggcaggt gcgggaaaaa ggaaaagttt tgcagattga cggagaaacc
     2521 ctgctgaaaa atcccgaatt gttgtcccgc gcgatgtatt ccgcagtggt ctcaaacaat
     2581 attgccggta tccgcgttat tttgccgatt tacctacaac aggcgcagca ggataagatg
     2641 ttggcacttt atgcacaagg gattttggcg caggcagacg gtagggtgaa ggaggcgatt
     2701 tcccattacc gggaattgat tgccgcccaa cccgacgcgc ccgccgtccg tatgcgtttg
     2761 gcggcagcat tgtttgaaga caggcagaac gaggcggcgg cagaccagtt cgaccgcctg
     2821 aaggcggaaa acctgccgcc gcagctgatg gagcaggtcg agctgtaccg caaggcattg
     2881 cgcgaacgcg atgcgtggaa ggtaaacagc ggcttcagcg ttacccgcga acacaatatc
     2941 aaccaagccc cgaaacggca gcagtacggc aaatggactt tcccgaaaca ggtggacggc
     3001 acggcggtca attaccggct cggcgcggag aaaaaatggt cgctgaaaaa cggctggtac
     3061 acgacggcgg gcggcgacgt gtccggcagg gtttatccgg ggaataagaa attcaacgat
     3121 atgacggcag gcgtttccgg cggcatcggt tttgccgacc ggcgcaaaga tgccgggctg
     3181 gcagtgttcc acgaacgccg cacctacggc aacgacgctt attcttacac caacggcgca
     3241 cgcctttatt tcaaccgttg gcaaaccccg aaatggcaaa cgttgtcttc ggcggagtgg
     3301 gggcgtttga agaatacgcg ccgggcgcgt tccgacaata cccatttgca aatttccaat
     3361 tcgctggtgt tttaccggaa tgcgcgccaa tattggatgg gcggtttgga tttttaccgc
     3421 gagcgcaacc ccgccgaccg gggcgacaat ttcaaccgtt acggcctgcg ctttgcctgg
     3481 gggcaggaat ggggcggcag cggcctgtct tcgctgttgc gcctcggcgc ggcgaaacgg
     3541 cattatgaaa aacccggctt tttcagcggt tttaaagggg aaaggcgcag ggataaagaa
     3601 ttgaacacat ccttgagcct ttggcaccgg gcattgcatt tcaaaggcat cacgccgcgc
     3661 ctgacgttgt cgcaccgcga aacgcggagt aacgatgtgt tcaacgaata cgagaaaaat
     3721 cgggcgtttg tcgagtttaa taaaacgttc tgattgctgt tccttttcgg aggaaaccct
     3781 gccggcggcg gtatcacggc gggcatcggc ggctttcggg cggtgctttg cgtgccgccg
     3841 cgtgtgcgga aacgcattcc ggtttttccg gcataacggc gatgcgaggt aaaatgccgt
     3901 ctgaaacccg attcgggctt cagacggcat tgtcgcggtt gcggcaggcg ggttcaccag
     3961 attccgtcaa aggttttcgc gccgcgccaa aatttccacc tgtcggcggg tttgaaggtc
     4021 agcgtaccgc cgtgttgtcc gtccgtggtg atgtccagcc gtttgatttt gccgatgcgg
     4081 acggcttcgt aaagcggtgc gaaccagcgt tcttcccact gctgcaatat tgccgcatac
     4141 cgctccctgt cccctgtcag ggcggtcagg cgcaaatcgt ccataaacag gatatggtgc
     4201 gtgtcgggta ggtatgccgc cgtttcttca taggcgcgga agttgtcggg caatgcgcgg
     4261 cggtcggggt ggaaacggct ccaaaccgta tcggcgaaaa gcgtgccgcc ttgactgctt
     4321 ccgtgcagac tgtcccaaag ccacagcccg ttcaactcgg gcagcccgcg tttcttgcgg
     4381 ttatggttga cggggtgcgc cgccagccac atttggattt cggtttggac gcgcagccat
     4441 tccaacgcgt cttccccgtc cggctgatcg tccgcgccca acaatccgcc caagtccaaa
     4501 acgggcttcg cgccccagcg gtacgcgcaa ggaagggaaa ccagccataa ttcgggcagg
     4561 acgggaacga aacgccatgg aatgtcgccg taaaacgccg acaggtcgcg gcagagccgt
     4621 tccgcttcat ccgtaccgac gttcagatat tccgccgtta gcacatttgc ctgatgcatc
     4681 cccatctttt gccagacggg cgtggcgagc gcgacggctt cagacggcat attcaggctt
     4741 tgtgccgcgc gttccgccag tctgccgcac cacaaataac gcgcgtaaaa tgccgaagcc
     4801 gtgcagcttt ggcggtgcag cgagccgtat tgcaggattt tgttgaaggc gggcaggcag
     4861 agaggtattc gggtttcgtc ttcatccaaa ttgagcgagg gaatggcgag ggtgagtttc
     4921 atcgtttgac gtttcagaaa tgcaggtcag gcgcaacatt atagaggatt cggcgcaaac
     4981 gccgtcaaaa aggaacaata tggctgtctt cccactttcg gcaaaacatc ggaaatacgc
     5041 gctgcgcgcg cttgccgttt cgattatttt ggtgtcggcg gcatacattg cttcgacaga
     5101 gaggacggag cgcgtcagac cgcagcgcgt ggaacaaaaa ctgccgccgc tgtcttgggg
     5161 cggcagcggt gttcagacgg catattgggt gcaggaggcg gtgcagccag gcgactcgct
     5221 ggcggacgtg ctggcgcgtt cgggtatggc gcgggacgaa attgcccgaa taacggaaaa
     5281 atatggcggc gaagccgatt tgcggcattt gcgtgccgac cagtcggttc atgttttggt
     5341 cggcggcgac ggcggcgcgc gcgaagtgca gttttttacc gacgaagacg gcgagcgcaa
     5401 tctggtcgct ttggaaaaaa aaggcggcat atggcggcgg tcggcttctg aggcggatat
     5461 gaaggttttg ccgacgctgc gttcggtcgt ggtcaaaacg tcggcgcgcg gttcgctggc
     5521 gcgggcggaa gtgcccgtcg aaatccgcga atccttaagc gggattttcg ccggccgctt
     5581 cagccttgac ggtttgaagg aaggcgatgc cgtgcgcctg atttacgaca gcctgtattt
     5641 ccacgggcag caggtggcgg cgggcgatat tttggcggct gaagtcgtta agggcggcac
     5701 aaggcatcag gcgttctatt accgttcgga caaggaaggc ggagggggcg gcaattatta
     5761 cgatgaagac ggcagggtgt tgcaggaaaa aggcggcttc aacatcgagc cgctggtcta
     5821 tacgcgcatt tcttcgccgt tcggctaccg catgcacccc atcctgcaca cttggcggct
     5881 gcacacgggc atcgattatg ccgcaccgca gggaacgccg gtcagggctt ccgccgacgg
     5941 cgtgattacc tttaaaggcc ggaagggcgg atacggcaac gcggtgatga tacgccacgc
     6001 caacggtgtg gaaacgctgt acgcgcactt gagcgcgttt tcgcaggcgc aaggcaatgt
     6061 gcgcggcggc gaggtcatcg gttttgtcgg ttcgacaggg cgttcgaccg ggccgcacct
     6121 gcattacgag gcacgcatca acgggcagcc cgtcaatcct gtttcagtcg cattgccgac
     6181 acccgaattg acgcaggcgg acaaggcggc gtttgccgcg cagaaacaga aggcggacgc
     6241 gctgcttgcg cgcttgcgcg gcataccggt taccgtgtcg caatcggatt gaagtttgaa
     6301 ccggcgacga aaacaatgcc gtctgaaaat ctgcaaacag gttttcagac ggcatttata
     6361 gtggattaac aaaaaccagt acgtcgttgc ctcgccttag ctcaaagaga acgattctct
     6421 aagatgctga agcaccaagt gaatcggttc cgtactattt gtactgtctg cggcttcgtt
     6481 gccttgtcct gatttttgtt aatccaccat acagtcgatt aaaataaaac taagccaagg
     6541 aagcactgcc gtcattcccg tacgggcggg aatccggaca ccacggtacg gaaacccatc
     6601 cgccgtcatt cccgcgaaag cgggaatcta gaaatgcaac gcggcaggag tttatcggaa
     6661 atgatggaaa cccaacgcac cggattccca ctttcgcggg aatgacgaag taggtaggaa
     6721 tccggattta tccgttccga cagtgtttgc aaataaaaga aaacccaacc gtcccgattc
     6781 ccggcagggc tgttttacgg attttgcagc gagggcgcgg ggcggtcttg cgcctgtttg
     6841 gtttgcaggg tcgtcagttt tttcgtcagc agattcagaa tcacgccgta ggcgggcagg
     6901 aaaaacagac cgcagacggt gagtttgaac aggtaatcga caaaagcgat gccctgccag
     6961 tttgccgcca taaattcatc gctgcttgca tagaaggcaa cggcgaaaaa taccaacgta
     7021 tccaaggcgt tgccgatgac ggttgatgcg gtcggtgcaa tccaccacgc tttcagacga
     7081 cgtaatttgt tgaacacaaa aatatcaagg atttgtccga gcgcgtaggc ggcaaagctg
     7141 gccaaggcga tgcgtccgac aaaggtgttg aattcggata gtgcgcccaa gcctgtccaa
     7201 ctgccgttgt ggaacaaaac ggaaaagacg taggaaagca aaagggcggg gaacatcacc
     7261 caaaagataa tccgccgtgc caagtgcgaa ccgaaaatgc ggacggtcag gtcggtggcg
     7321 aggaagatga aggggaagga aaacgcaccc caagtggtgt ggatgccgaa aatttggaag
     7381 gggaactgca ccagatagtt gctggcggcg atgatgagga tatggaaaag caccagccag
     7441 aagagtgcct tctgttgctg tgcggcagta aatgcgtaca taaaaatctt tcggaaaggc
     7501 gttcagacgg cataccgtat cgaaggaatg ccgtctgaaa tatgggaagg atggtttatt
     7561 gtgcgtcgtg ctcaaacagg cgtttacgcg ccaatgcttc aaactcggtg cctgctttgc
     7621 cgtaattggc gaaaggatga atggcgatgc cgccgcgcgg tgtgaactcg ccgaatactt
     7681 cgatgtattt cggatccatc agggcaatga ggtctttcat gatgatgttg acgcagtctt
     7741 catgaaaatc gccgtggttg cggaagctga agaggtagag tttcagggat ttgctttcca
     7801 ccattttgat gtgcggaatg tagcggatgt agatggtggc gaagtcgggc tgcccggtca
     7861 tggggcagag gctggtgaac tctgggcaga cgaatttgac gaaatagtcg ttgtcgggat
     7921 gtttgttgtc gaatgcttcg agaatttcag gcgcgtagcc ggtcggatat cgggttttct
     7981 gattacccaa aagcgagatg ccttgcagct cttcggtatt gcgggacata gggtttcctt
     8041 agttttttag tgtgggaggt tttcgaacca cggggcggcg attgtaatat aagcggcggt
     8101 atctgtgtag ttttcttcag acggcatggt ttggacggcg gcgttttccg t
//
LOCUS       DAWFKN010000085         8132 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFKN010000085 DAWFKN010000000
VERSION     DAWFKN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.085"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(67..291)
                     /locus_tag="WGR46_001802"
     CDS             complement(67..291)
                     /locus_tag="WGR46_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0856686.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WGR46_001803"
     CDS             complement(292..1680)
                     /locus_tag="WGR46_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0856687.1"
                     /translation="MNAVVVAVIVMLSLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGR46_001804"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGR46_001804"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0856688.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2577..3038)
                     /locus_tag="WGR46_001805"
     CDS             complement(2577..3038)
                     /locus_tag="WGR46_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856689.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR46_001806"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR46_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0856690.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4765..4868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4934..6157
                     /gene="cytX"
                     /locus_tag="WGR46_001807"
     CDS             4934..6157
                     /gene="cytX"
                     /locus_tag="WGR46_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0856691.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6154..7254
                     /locus_tag="WGR46_001808"
     CDS             6154..7254
                     /locus_tag="WGR46_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0856692.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEDLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7275..7892
                     /gene="thiE"
                     /locus_tag="WGR46_001809"
     CDS             7275..7892
                     /gene="thiE"
                     /locus_tag="WGR46_001809"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0856693.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHSDE
                     LKREIARCAAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1749 a         2505 c         2189 g         1689 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact cataatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagtttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 cgcaaacgca ccgagcatcg cataagaaag cgcaatcttc gcgccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcgacagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 gaaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaag tccggcaggg tttccactcc cgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatct gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgtgctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagtgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaagtg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcaattttag gcagttttga caagcccaac cattcgtcaa
     2581 acgtcatttt ttatcccgtc tgccgccgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaag tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagt ccggcatcaa tccgcaaagt cggttgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gaccgttgcc gataatggtc gcgcctttga cgggatattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccgttttc cataaaggta ttggtcatgc gcggcatagg
     3481 ggcggaggcg tagctttcgc ggcggccgtt gccggtcgat tgcgtacccg tcaggcgggc
     3541 gttggtttcg tcctgcatat agccggtcaa aataccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcgccctg
     3661 atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgccg gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac cgatgaacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg ataacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttgtc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcgtaatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatatagtg gattaacaaa aaacagtacg
     4621 gtgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4681 ttttgttaat ccactataga aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg
     4741 ccccctgctt ttgtgtacac ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag
     4801 attgcgaaag cagaatccgt agaacctgtc ggggtaatgc ctgcgtagga aacaaaacct
     4861 ccccgccctt tcgggcttcc gttccctttt ccgcacttcc ccgccccatt ttcatgtttt
     4921 ttaaggactt gatatgtcgg gcaatgcctc ctctccttca tcttcctccg ccatcgggct
     4981 gatttggttc ggcgcggcgg tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc
     5041 tttgggctgg cagcgcggtc tggcggctct acttttgggt catgccgtcg gcggcgcgct
     5101 gttttttgcg gcggcgtata tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg
     5161 cctgtcgttc ggcaaacgcg gttcagtgct gttttccgtg gcgaatatgc tgcaactggc
     5221 cggctggacg gcggtgatga tttacgccgg cgcaacggtc agctccgctt tgggcaaagt
     5281 gttgtgggac ggcgaatctt ttgtctggtg ggcattggca aacggcgcgc tgattgtgct
     5341 gtggctggtt ttcggcgcac gcaaaacagg cgggctgaaa accgtttcga tgctgctgat
     5401 gctgttggcg gttctgtggc tgagtgccga agtcttttcc acggcaggca gcaccgccgc
     5461 acaggtttca gacggcatga gcttcggaac ggcagtcgaa ttgtccgccg tcatgccgct
     5521 ttcctggctg ccgctggccg ccgactacac acgccaagca cgccgcccgt ttgcggcaac
     5581 cctgacggca acgctcgcct atacgctgac gggctgctgg atgtatgcct tgggtttggc
     5641 agcggcgttg ttcaccggag aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg
     5701 tgcggcaggc attttggcgg tcgtcctctc caccgttacc accacttttc tcgatgccta
     5761 ctccgccggc gtaagtgcga acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt
     5821 cggcgttacc ctgatcggca cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa
     5881 cttcctactg cttatcggct cggtatttgc gccgatggcg gcggttttga ttgccgactt
     5941 tttcgtcttg aaacggcgtg aggagattga aggctttgac tttgccggac tggttctgtg
     6001 gcttgcgggc ttcatcctct accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg
     6061 tctgaccgcc cccgtaatgt ctgccgttgc cattgccacc gtatcggtac gccttttctt
     6121 taaaaaaacc caatctttac aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc
     6181 ggcctctcgg gaaggctgac cgcactgcag cttgcagaac aaggttatca gattgcactt
     6241 ttcgataaag gctgccgccg gggcgaacac gccgccgcct atgttgccgc cgccatgctc
     6301 gcgcctgcgg cggaagcggt cgaagccacg cccgaagtgg tcaggctggg caggcagagc
     6361 atcccgcttt ggcgcggcat ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac
     6421 ggcagcctga ttgtgtggca cgggcaggac aagccattat ccagcgagtt cgtccgccat
     6481 ctcaaacgcg gcggcgtagc ggatgacgaa atcgtccgtt ggcacgccga cgacatcgcc
     6541 gaacgcgaac cgcaactcgg cggacgtttt tcagacggca tctacctgcc gaccgaaggc
     6601 cagctcgacg ggcggcaaat attgtctgca cttgccgacg ctttggacga actgaacgtc
     6661 ccttgccatt gggaacacga atgcgtcccc gaagacttgc aagcccaata cgactggctg
     6721 atcgactgcc gcggctacgg cgcaaaaacc gcgtggaacc aatcccccga gcacaccagc
     6781 accctgcgcg gcatacgcgg cgaagtggcg cgggtttaca cacccgaaat cacgctcaac
     6841 cgccccgtgc gtctgctcca tccgcgttat ccgctctaca tcgccccgaa agaaaaccac
     6901 atcttcgtca tcggtgcgac ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt
     6961 tccgggctgg aactcttatc cgcactctat gccgtccacc ccgctttcgg cgaagccgac
     7021 atcctcgaaa tcgccaccgg cctgcgcccc acgctcaacc accacaaccc cgaaatccgt
     7081 tacaaccgcg cccgacgcct gattgaaatc aacggccttt tccgccacgg tttcatgatc
     7141 tcccccgccg taaccgccgc cgccgtcaga ttggcagtgg cactgtttga cggaaaagac
     7201 gcgcccgaac gcgatgaaga aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt
     7261 ctgaaaggaa ccccatgacc ttcctgccac tcaaatcccc gctcaaattc tacgccgtcg
     7321 tccccaccgc cgattgggtc gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc
     7381 gctgcaaggc cctgcacagc gatgaattga aacgcgaaat cgcccgctgc gccgcagcct
     7441 gtcagggcag ccatacgcag cttttcatca atgaccactg gcgcgaggca atcgaagcag
     7501 gcgcgtacgg cgtgcatctc ggacaagaag acatggacac cgccgacctt gccgccatcg
     7561 ccgccgccgg tttgcgcttg ggtttgagta cgcactccgt tgccgaactc gaccgcgccc
     7621 tgtccgtaca ccctagctac atcgccagcg gcgcgatttt cccgaccacg accaagcaaa
     7681 tgcccaccgc cccgcaaggc ttggacaaac tgcgcgaata cgtcaaacag gcaggcggca
     7741 cgcccgtcgt cgccattggc ggcatcgatt tgaacaacgc ccgagccgta ctcgccaccg
     7801 gcgtttcctc actcgccgcc gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg
     7861 ttaaagcgtt tcaggctttg tgggatggat aaaaccgaaa gaagaaaatt caattgccgt
     7921 gtcggcaaaa cttagcccgt tatcgcaaat atgcttaact ttaaatgtgg catatcatca
     7981 aattccgtca ttcccgcgca agcgggaatc cgccttaaaa cttgagaaac catcatttga
     8041 aaaacagttt ccgaatttca aaaatggatt cccgcctgcg cgggaatgac ggcaaccggc
     8101 cggttgcgta tcaaaaaata aagtaattcg gc
//
LOCUS       DAWFKN010000086         8107 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFKN010000086 DAWFKN010000000
VERSION     DAWFKN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.086"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(208..936)
                     /gene="rmpM"
                     /locus_tag="WGR46_001810"
     CDS             complement(208..936)
                     /gene="rmpM"
                     /locus_tag="WGR46_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0856694.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1300..2250
                     /locus_tag="WGR46_001811"
     CDS             1300..2250
                     /locus_tag="WGR46_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0856695.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2363..3097)
                     /gene="fnr"
                     /locus_tag="WGR46_001812"
     CDS             complement(2363..3097)
                     /gene="fnr"
                     /locus_tag="WGR46_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0856696.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3302..4723
                     /gene="hemN"
                     /locus_tag="WGR46_001813"
     CDS             3302..4723
                     /gene="hemN"
                     /locus_tag="WGR46_001813"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0856697.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5076..6650)
                     /locus_tag="WGR46_001814"
     CDS             complement(5076..6650)
                     /locus_tag="WGR46_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0856698.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6975..8075
                     /locus_tag="WGR46_001815"
     CDS             6975..8075
                     /locus_tag="WGR46_001815"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0856699.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
BASE COUNT         2035 a         2261 c         2005 g         1806 t
ORIGIN      
        1 ggcaatggga cacaaacctg aatctgaatc tgaacctgca ccccgattct gacgatagcc
       61 gtttcagaaa accacccaaa aaaacaaacc ccgcaaaagc gggggtttgt ttcacatcat
      121 cgatgccgac cgacgtttca cgaatattgc aacagtacct ggtacaaaat ccactaacct
      181 catgcatcgg caagatattg cctagcctta gtgttggtga tgattgtgtg ccggcacaac
      241 ctgacgggtt acgatgctgc ggattttcac atccacacgg cggtcaggtt cgatacatgc
      301 aatcagagcc tcacgttttt tggctttaga gactttcgca cccagtttgg caacttcggc
      361 ttcacaaact tgagtcattt gcgcttgaga ttcgcccaag ccgacagcag aaattctaga
      421 aacaggtacg ccgttgctga ccaggttgtt tgccactacg tatgcgcggc gttcggacag
      481 ggcctgattg tatttgtcag aacccataaa gtcggtatgg ccttcgacgc ggacagattg
      541 gatattggtt tgacccaggc gttgcgccaa tactttcagg ttgtcttgag cttcggcgcg
      601 caatgaatcc ttatcgaaac cgaacagggt tttggcagac agggaaatgg tttcatcaac
      661 atattgcgga gcctgctcca caacgacgac aggcgcgggt gcgggttcgg gttctggctc
      721 gggttcgggg gcagcaaccg catcgccgca ttctacgcga ccttggcttg ctttatcaaa
      781 gtaggcgttt ttccagcatt cgccatagtt gttgcgtact atttcgttcg actggccgct
      841 tacggtgtaa ccctgaacgg acgcctcgcc cgcaacagca gtgccggaag cgagcaatgc
      901 aacgaataat gcgcttaatt tcagctgttt ggtcatttta ttccctcatt aaatttgtac
      961 agcagataca gaagcaactg ctgaattgcc aacatacagg ccgctgtaga aacatggcgg
     1021 caaatcggat atttcagtct ccactataaa gaagcagggg gaggactgtc aatactcaac
     1081 gcctgaaaac aaccgtaaaa cagcctgttt cctgatagtc tatcatgccg aaaaatcaat
     1141 tccgtgtcat tactttaggg ggattttttc catatcgcac gattgccgtt gcacaaaaac
     1201 aacaaacaca acggcaaagc cccataccgt accggcaaga aaatatgata aattataaac
     1261 aatgttcagc cacccgacac agacccacac cgacccgcca tgaaattaca acaattgaaa
     1321 tacgccttag aagtttacca gcacaacctg aacgtttccg aagcggccga agccttgttt
     1381 acttcgcaac ccggcatctc caaacaaatc aaattgctgg aagaagaaat cggcattcag
     1441 atttttatcc gcagcggcaa gcgcgtggtt tcggtctcgc agccgggcaa ggtggttttg
     1501 gatattgcgg aacgtatttt acgcgatgta cagaacatta aaaatatcgg cagcgagttt
     1561 accggacagg acagcggttc gctgacggtt gccacgacgc atactcaggc acgctatgcc
     1621 ctacccttga ttgttgccga ttttgtgaaa cgctatccga aagtcaacct gaccatcaaa
     1681 caggggagcc ctgccgccat cgcacgtatg gttacctcag gcgaggcgga cttggcgatt
     1741 gttacggaac ggatagacga ccatcccgaa ctgggaaaac ttccctgcta tgactggact
     1801 catgcggtta tcgtaccgaa cgaccacccc ttgctcgaat gcagaaaccc cctccgcatt
     1861 gaagatttgg cgaggtttcc gctgattact tatgaatttg cattcaatgc gggcagcagc
     1921 atcgcgcggg cattttccaa agcccgtttg gaacagccgg atgtcgcatt ggcggcggcg
     1981 gatacggacg tattgaagac ttatgtgcgc ttgggtttgg gcgtgggact gatggcgaaa
     2041 atggcgtaca acccggatac ggacggcgat ttgcagcttg tggatgcggc acacctgttc
     2101 gagccgtcgc cgacgtggat tgctttgcgc agcgatactt atttgcgcgg atatgcctac
     2161 gactttatcc aagcgtttgc gccgcacctg acacgcgaga aggtggatag gattctgtac
     2221 acgcccatca gcgaggattt ttcgatttag gcggctgccg gttttcagac ggcactttgc
     2281 ggcagataca acaaacagga cagatgtttt cgtctgccct gtgtttattg agaatgctgt
     2341 ctgaaatgtt cgtacgggtt aatcaaatgg cgtgcgagca gccggacacc atttttttca
     2401 acacctgcag attgaggatt ttgatgtgct tatgctcgac ggaaatcaat ccttcctgat
     2461 ggaacttgga taatgtgcgg ctgacggttt caagtttcag cccgagataa ctgccgattt
     2521 cttcgcggga cattcttaag atgaagtcgt tggcagcaaa acctcgggaa taaaggcgtt
     2581 gggaaaggtt cagcaggaag gcggcaatcc gctcttcggc gcgcatattg cccaacagca
     2641 gcataacacc ttggtcgcgc acgatttcac ggctcatcat gcggaagaag tgcgtacgca
     2701 ggctggggat gttttgcccc agttcttcga tgtgggtaaa cggcagttcg cacacttcgc
     2761 tgtcttccaa ggcgaccgcg tcgcaactgt gcacatggga acagatgccg tccatgccga
     2821 tgagttcgcc cgacataaag aaacccgtta cctgatcgcg gccgtcctga ctggcgacgg
     2881 ttgttttgaa gaagcccgaa cggatggcaa agagcgaggt aaaggcttcg ccgacacaga
     2941 acaggtattc gccctttttc aggcggcggc tttgacggat gacggcatcg agttggctga
     3001 gctcgttggg cagcagcccg acaggcaggc agagttcccg caaagaacag gaagaacaca
     3061 gcgttttcat ctgatgtgta gtattatgcg aagccatacc gtaccttttt gtgcgctttg
     3121 ccccatcatg attatagtgg attaaattta aaccagtacg gcgttgcctc gccttgtcct
     3181 tatttaaatt taatccacta tatgtgctta ttgacacata tcaagacagg tttatcatac
     3241 tgtggcattc taccaaacta tccaaaattt gactaacatc ataaaaccgc acacacacac
     3301 catgaaaatc attcagatac agaacaatca caatgtaaac gatgaccgcc ccgagtttga
     3361 ccgcgcgctg attgccagcc tgcccgccag cggcccgcgc tacacttcct accctaccgc
     3421 cgaccgtttc catgacggtt tccgcgaagg cgaatatatc aaagctttac atttgcgcgg
     3481 tatgggcgcg ttaaacaaac cgctttccct ttacattcac attccgttct gcaacaccat
     3541 ctgctactac tgcggctgca acaaaatcat caccaaagac aaaagccgcg ccgatgccta
     3601 catcgaatat cttgaaaaag aaatggaact gctcgctcca catctgaacg gacggcacca
     3661 gcttgcccaa ctgcacttcg gcggcggcac gccgaccttt ttgagcgacg aacagatcga
     3721 acgtgtcttc cgcatgatac gcaaacattt cgagttaatc cccaccggcg aatactccat
     3781 cgaaatcgac ccgcgcaaag tcagccgcga caccgtcctc atgctcggca ggctcggctt
     3841 caaccgcatg agcatcggca ttcaggattt cgaccccaaa gtgcaggcgg cggtcaaccg
     3901 catccaaagt tacgaagaaa ccaaagaagt catcgatgcg gcgcgcgaag cggggttcaa
     3961 atccgtcagc gtcgatttga tttacggcct gccgcaccag acttcggaaa gcatcaaaac
     4021 caccatcgat accgttttgt cgctcgatcc cgaccgcctc gccctttatc actacgccca
     4081 cctgccgcac gtgttcaaac cgcaacgccg catcgatacc gtcgccgttc ccgacagcga
     4141 agagaagctc gatatgctgc aatactgcgt ccaaacccta accgaacgcg gctacgtctt
     4201 catcggcatg gaccatttcg ccaaacctga cgacgaactc tccatcgccc tcaaagaagg
     4261 cttcctccag cgcaacttcc aaggctattc gacctacgcg gattgcgatt tggtcgccat
     4321 cggcgtgtcg tccatcggca aaatcggcag cacctattcc caaaacgaac gcgacatcga
     4381 tgcctactat gccgccatcg acgaaggcag actgcccatc atgcgcggtt atcagctcaa
     4441 tcaggacgac atcctgcgcc gcaacatcat tcaggatttg atgtgccgtt tcgcgctcga
     4501 ctatcggatt tacgaaagta tgttcggcat cccgttcgac cgctacttca aagacgaact
     4561 ggcggatttg gaaaaactcg ccggtttggg attggtgcgc ctgaacagcc acgggctgac
     4621 cgttaccccg aaaggacgct tcctcatccg caacatcgcc atggtcttcg attaccacct
     4681 gcgccataaa gaaaccaagg cgaaatattc gcaaacggtg tgattgcgtc taacgtacaa
     4741 atgccgtctg aaaggctttt tcagacggca ttttgctgcc ggcaggataa gtgttttcaa
     4801 gaacaggcgg cggcatatca taacgttccg cacctttgtg tccgaccgtt ccgaaaccaa
     4861 gatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta ctatttgtac
     4921 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc actataccaa acggcatatc
     4981 ccgacagaac agattgtgca taaggcacaa gcccgcacat tccatcaaca aaatgccgtc
     5041 tgaacacggg ttcagacggc atcagtattt tacaatcaga atactgcctg taaaaccaag
     5101 tagcagacaa ccgacaatac ggcggcggca ggcagggtaa tgacccacgc caaaccgatg
     5161 ggcttcatca gtttccagtt ggcattgcgg ttgaccagac cgatgccgag taccgcgccg
     5221 accaagatat gcgtactgga cacgggcagc cccatcagcg acgcgcccat cacgacggag
     5281 gcggcggaca gttcggcggt aaaacccgaa gcaggatgca tttccgccaa actcgtaccg
     5341 acggttttaa tcacctcttt accgacaaac cacaaaccga caatcagcgc gataccgaaa
     5401 gtcagcatcg caatcggggg gacgacattt tgcgcggcaa cgctgttggt acgcaaaaca
     5461 tccataatcg cggcaaacgg accgatggcg ttggcgatat cgttcgcacc gtggctgaat
     5521 gcgaagccgc aggcggtaaa gacctgcatc catgaaaaca tctgaaaggt cgatttgccc
     5581 aagtctttac gcttgaggct tttggcaaac acaaacgtcc ccatccacac cgccgcgcct
     5641 atcataaaga tggtcaggaa gctgttgacg ttgctcatcc ccaaatgcag gtttttcaag
     5701 cccttgaaaa tcagcatagc ggaaatcatc atcgcgccga acgaagcgat aaagggaatc
     5761 caagaatgca gcgctttgta ggaatcgaca ttgtttttac ggttgtcgaa cgcataaaga
     5821 ccgcggtaat actccgattg cagctcttgc ggatcgaatt cgggttcgtc gtaaatttgc
     5881 gcgtcgtgcg ccattttggt ggcgtactcg actttttcgg cttcggacaa accctcgaaa
     5941 aacaggcggt gccgttcttt ataggccttt ttttcctgct tgatgccctt gagcgtgcct
     6001 tccgcccaag cgttgtaatc taagacgttt ttcttgacgc gcgaaaacag aaaataggac
     6061 accgcgccgc ccaacacggg cgacaatacc caagaaacac caataccgcc cagcttgccc
     6121 caacgtatca aatcgcccga tgcggcatcg ttcattaccg ccatacatac cgcgctgccg
     6181 acaatgccgc cgataatgga atgggtggta gataccggaa gccctttttt cgaggcaaac
     6241 aacagccaca acgccgccgc caaaagcgcg gacatcataa taaacacaaa ctgtatgggt
     6301 tcgaaatcaa cacccttcaa atcgacgatg cctttgcgta tggtattggt tacctcgccg
     6361 cccgcgatga ccgcgccgct gacctcaaat accgccgcaa tcagcaaagc ctgcgggatg
     6421 gtcagcgtac ccgcaccgac actggtgccg aaagaattgg caacatcgtt gccgccgatg
     6481 ttgaacgcca taaacacgcc gaacatcgtg gcaataatga ataaggcggc gtaattgtaa
     6541 tgggtataac ccaagcccca ataaataaaa tagccgactg tacctaccag catggcggca
     6601 aagacggcgt tgatggtttt aacatttgcg ctcatttgga tttgagccat ggcaatcttc
     6661 ctttaatgtg gtggaaatca tgagtgttgc cgacactgta ctgctgcctc cggtcgaact
     6721 gccgtttcgg gatatggcag acgcatcctt ccttctgaac cgcatccgaa tacccccaaa
     6781 cctgaccgaa tgcaaaactg ctgccggaaa cggtttgtat gacacttcta ctactggatg
     6841 ttgcgggcgc attataatat ttttccatcc gtcttgaaac atttatttac actttattta
     6901 cactgcggcg gcaaatcggt atacgagcgt caatacacgt taaaatggcg ttttgcacca
     6961 gtttgggagt gatgatggaa acacagcttt acatcggcat catgtcggga accagtatgg
     7021 atggggcgga tgccgtgctg gtacggatgg acggcggcaa atggctgggc gcggaagggc
     7081 acgcctttac cccctatccc gacaggctgc gccgccaatt gctggatttg caggacacgg
     7141 gggcggacga actgcaccgc agcaggattt tgtcgcaaga actcagccgc ctgtacgcgc
     7201 aaaccgccgc cgaactgctg tgcagtcaaa acctcgcgcc gtccgacatt accgccctcg
     7261 gctgccacgg gcaaaccgtc cgacacgcgc cggaacacgg ttacagcata cagcttgccg
     7321 atttgccgct gctggcggaa ctgactcaga tttttaccgt cggcgacttc cgcagccgcg
     7381 accttgcggc cggcggacaa ggtgcgccgc tcgtccccgc ctttcacaaa gccctgttcc
     7441 gcgatgacaa agaaacacgc gtggtactga acatcggcgg gattgccaac atcagcgtac
     7501 tcccctccga cgcacccgcc ttcggcttcg acacagggcc gggcaatatg ctgatggacg
     7561 cgtggacgca ggcacactgg cagcttcctt acgacaaaaa cggtgcaaag gcggcacaag
     7621 gcaacatatt gccgcaactg ctcgacaggc tgctcgccca cccgtatttc gcacaacccc
     7681 accctaaaag cacggggcgc gaactgtttg ccctaaattg gctcgaaacc taccttgacg
     7741 gcggtgaaaa ccgatacgac gtattgcgga cgctttcccg ttttaccgcg caaaccgttt
     7801 gcgacgccgt ctcacacgca gcggcagatg cccgtcaaat gtacatttgc ggcggcggca
     7861 tccgcaatcc tgttttaatg gcggatttgg cggaatattt cggcacacgc gtttccttac
     7921 gcagcaccgc cgaactgaag ctcgatccgc aatgggtgga agcagccgca tttgcgtggt
     7981 tggcggcgtg ttggattaat cgcattcccg gtagttcgca caaagcaacc ggcgcatcca
     8041 aaccgtgtat tctgggcgcg ggatattatt attgagcgca aatgccgtct gaaagcccgt
     8101 tccgccg
//
LOCUS       DAWFKN010000087         8090 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFKN010000087 DAWFKN010000000
VERSION     DAWFKN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.087"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(113..1831)
                     /gene="argS"
                     /locus_tag="WGR46_001816"
     CDS             complement(113..1831)
                     /gene="argS"
                     /locus_tag="WGR46_001816"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0856700.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            complement(1923..3131)
                     /gene="pncB"
                     /locus_tag="WGR46_001817"
     CDS             complement(1923..3131)
                     /gene="pncB"
                     /locus_tag="WGR46_001817"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0856701.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            complement(3172..4026)
                     /locus_tag="WGR46_001818"
     CDS             complement(3172..4026)
                     /locus_tag="WGR46_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0856702.1"
                     /translation="MLPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            complement(4271..5047)
                     /locus_tag="WGR46_001819"
     CDS             complement(4271..5047)
                     /locus_tag="WGR46_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0856703.1"
                     /translation="MSNNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIRHLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVECKQPFVLSGGAGGQKDP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            5264..5728
                     /locus_tag="WGR46_001820"
     CDS             5264..5728
                     /locus_tag="WGR46_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0856704.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            5810..6538
                     /gene="rph"
                     /locus_tag="WGR46_001821"
     CDS             5810..6538
                     /gene="rph"
                     /locus_tag="WGR46_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0856705.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNSKVICTA
                     SVDENVPSFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALHIAVGKLISDGILTENPIRE
                     AVAAVSAGIVGGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLEELG
                     KLIGLAEKGIRELVRYQQNALSAA"
     gene            6739..7956
                     /locus_tag="WGR46_001822"
     CDS             6739..7956
                     /locus_tag="WGR46_001822"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0856706.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAIADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
BASE COUNT         1802 a         2080 c         2334 g         1874 t
ORIGIN      
        1 cgtttgtttg tttcgcattt tgggaggggc ggcggatacg gtgtatcgcg ccgctttttt
       61 cagattcgga taaggatggc gaggctgttg tccgcaatcg ggcggtgcgg ttttacatta
      121 cgtccaacac atcgatgccc agcaaatcca agccttgttt cagcgtgtcg ccggtcagct
      181 tcgccagttg caggcggctg ttgcggcttg cgctttcggc tttgagtatc ggacaggctt
      241 cgtagaagcg gctgaacagg gtcgcaattt gatagaggta ggcggcgagg tagtgcggat
      301 acgccgtgtc cgccacgctt tgcagcacgt cttcgaattt cagcagctcg gcggcaagct
      361 gtttttccag cggttcgctc aaaacagtcg gtgcggttgc gtcccattca ccggctttgc
      421 ggaacacgct ttgcacgcgg gtgtaggcat attgcaggta gggggcggtg ttgccttcaa
      481 acgaaagcat agcgtcccag tcgaacacat aattgctggt gcggtttttg ctcaagtcgg
      541 cgtatttgac cgcgccgatg ccgacggttt tgccgatttt ggcggcttcg tccgcgccca
      601 attcgggatt tttctctttc accaaagcgg cggcgcgttc gacggcttcg gtcagcagat
      661 cgaccagttt tacagtgtcg ccgctgcgtg ttttgaacgg tttgccgtct ttgcccatca
      721 tcgtgccgaa gccgacaaat gccgcgccga cgttttccgg cagatagcct gctttgcggg
      781 aagtggtaaa gagttgctca aagtgcaggg cttggcggtg gtcgacgacg tacagcaggc
      841 ggtcggcatg aagcgtgccg acacggtagc gcaaacacgc caagtcggtg gaagcataaa
      901 ggaagccgcc gccttgtttt tgcacgataa acgcggcggg ttcgccttct ttgtttttga
      961 actcgtccaa gaatacgact ttcgcgccgt cgtcttcgac cgccagacct ttttgaacca
     1021 aatcatcgac cactggctgc aaatcgtcgt tgtacttcga ttcgcccgcc acgtcttcgg
     1081 ggcgcagctt caagcccagc gtgtcgtaaa cggcttgggc gtgcgagagc gaaatatcga
     1141 caaactgttt ccacaatgcc aaaacggttt catcgccgcc ttgcagcttc acaacgtatt
     1201 cgcgcgcggt gtcggcgaag gcaggatctt cgtcaaagcg cactttggcg gcgcggtaaa
     1261 actgctccaa atccgccagc tcaaacgcgg cattgtcttt ttgctgctcg accaaataag
     1321 cgaccaacat gccgaactgc gtaccccagt cgccgacgtg gttttgacgg acgacggtgt
     1381 tgcccataaa tgccaacacg cgcgaaatgc tgtcgccgat gatgctggaa cgcaggtgac
     1441 cgacatgcat ttccttcgcc agattgggcg aggaatagtc gatgacgacg gtttgcggtt
     1501 tgtcggtttt cgctatgccg aaacgcgcgt cgttcaaggc cgtctgaatg ttttgcgcga
     1561 gaaattcggg gcgcaggcgc aggttgataa aaccgggacc ggcgacttcc gcgctttcga
     1621 tgacggcgtt gcctgccaat gcgtcggcga ccttttgcgc cagttcgcgc gggttttgtt
     1681 tggctttttt cgccgcaccc atcacgccgt tgatttggaa atcgccgtgt tcggcatttt
     1741 tggtcggctg caaaacaacg gggctgccgg cgatgcctgc ggcggcaaag gcggcggcgg
     1801 cttcgtgttc gacggtttga tgtaggttca tgaagttcgg tctttcggtt aaattcaaat
     1861 caggcggtat tttaaaagat tttatgcggc agaaacagga attgtgcgct ttttctgccg
     1921 gtttacggcg tttcgggttc gggtacgtcg aacacttggc gcaggtaggc gaggaaggtg
     1981 ctgttgttgg tcatggtttt gcccggagag tcggacagct tggcgacgga ctgcccgttg
     2041 cattcgacca gtttcaagac gatattcaaa ggcgtatgcc ccatatcgtt ggtcaggttg
     2101 gtgccgatgc cgaagccggt tttgaagcgg tctttgaaat attggtgtaa tgcccaagag
     2161 cgttcgatgt ccagcccgtc ggagaaggtc agcattttgg tgcggctgtc gattttgagc
     2221 ttttgatagt gggcgtaggc tttgtcgccc caaacgtaag ggtcgccgct gtcgtggcgc
     2281 agcccgtcga aaagtttggc gaaatagagg tcgaaatcgc gcaggaaggc atccataccg
     2341 accacgtcgg tcagggcaac gcccaaatcg ccccggtatt cgtgcaccca gctttcgagc
     2401 gcggcctttt ggaaattccg caggcgtacg tcgagggcct ggaaggcttg gaggaactcg
     2461 tgcgccatgg tgccgatggg ggtgatgccg agttttttgg cgagaaagac attgctggtg
     2521 ccgcgcacga tgcttggggc ggcttcgagc agggtgcgga tgacgtgttc ctgccacgcg
     2581 agcttgtagc ggcggcgcgt gccgaagtcg gaaatcagga agggcggttc gtcggggttt
     2641 tgtgcggcgg cgatttcttt gaggcgcgcg gctttggctt gaagccggcg ttcgccttct
     2701 tctatgactg caggggtttc caggcggcgg aagtaaagtt cgttgacaat ggcgaggatg
     2761 aagatttcaa aaaacatcgc ctgtatcatc ggaccttcga tgcggatgtt cagacggcct
     2821 ttatcgtctg tgccgacttc gacaaagcgg cgttggagct ggaagagttc gagataatcg
     2881 acaaagtcgc ttttaatgaa acgcaggcta cggaggtagt cgagttcgtc gtgggtgaag
     2941 cgtagttggc agagcgcgtc gagttcgact tccaagtctt ccctgatgtc ggcaagcgga
     3001 tagacggtcg aggcgttgcg gcagcggaat tcgtaaaggc tgtgcgtctg cggaaactgg
     3061 tgcagaacca cttgcagcat agtgaatttg tagaggtcgg tgtcaagcag cgaatgtatg
     3121 atgccggtca tatggcgtgt ctcccgattt ttggtggcgg cttggggcgt attagaacac
     3181 atcgcgcccg ccccgtgtgc cggaaatgcc gtctgaatgc gccccctcat gattgaggct
     3241 gattcggatt tctccgaaac cgacttcctg tacgattccg accaaacctt ctttggcaag
     3301 ctccaacagt gcgatgaagt tgacgaccac gtaagccgcg ccctgttcgg gattgaacag
     3361 gtcgtgaaac ctgcatatcc cgtgtccgtt caaacggcgc aggattgccg tcatttgcgc
     3421 gcgcacggag atggtttctt tgattacttc gtggctgcgc gtgtgttttg cccgagacaa
     3481 aatacccagc cacgcttgcg tcaagtcggt aatatagact tcgggcagct tggcttcgac
     3541 ggcaatttcc agcggcaggt aagcccacgc gaaatcccgt ccggctcggg gcagcgcgtc
     3601 caaaccctgc gccgccagct tcatctgttc gtaagccagc aggcggcgca ccaactcggc
     3661 acgcgggtcg gcttcttcgt cttcgacggt ttcggtacgc ggcagcagca ggcgcgattt
     3721 gatttcaatc agcattgccg ccatcaaaag atattccgcc gccaaatcaa actgataggt
     3781 ttctatttgg gcgatgtagt gcagatactg ctcggtaatc tttaccatcg gaatatccag
     3841 tacgtcgata ttctgtttgc ggatcagata cagcagtaga tccaaagggc cttgaaaact
     3901 gcccaatacg actttcagcg cgtcgggcgg gataaacaaa tcctgcggca aatcggtaac
     3961 gggttgaccg aatacccatg cgacagtgtg ctcggatggt gcagatgggg cagggatggg
     4021 cagcatggcg tgaatcggta agtggatttt aatataagtt tacaaaagaa aacaatctgc
     4081 cggcattcac gcataatgtg gtcgggcaga tgttcagacg gcattataac ggcttttaaa
     4141 tgcaaatgtt ttgaaaagga gtagattgtg aatacggttt taggcgtgcc gatgttggtt
     4201 tttctgaacc gacagtttga ttgccgcgtt tgttcaaatg ccgtctgaat cctgtttcag
     4261 acggcattgc ttattttttg cccgcgatgt gttccaccgc cgcctgtgcg caatatagcc
     4321 cgaacgaagc ggtaacgagc atgcttgcac cgtagccggc gcacgacaag ccttgcggag
     4381 cggcatcggt agaacacgcc gcgcccgatt gcggcggcgt gatgttttcg gttgagaaca
     4441 cgcacggcac gcgcattttg gctttcgtat cgcggctgaa gccgtagcgt ttccgcaagg
     4501 tgtagcgcag gttggcaagc agcgggtcgt gggttacgcg gctcaaatcg gcggtttgga
     4561 tcaacgccgg atctttttgt ccgcccgcgc cgccgctgag gacaaacggt tgtttgcatt
     4621 ccacaaaata agccgccatt gccgctttga cgcgcacttg gtcgatcgcg tcgatgacga
     4681 aatcaaaacc ttttccgaag tattccggca aattgtcttc ggtaacgaaa tcttcaattt
     4741 caaatacttc gcattgcgga ttgatttgtg taatgcgttc gcgcaaggcg gtaacttttg
     4801 ctttgccgaa gtcgccggtc agggcgtgca gctggcggtt gacattcgat tcggcaacgt
     4861 tgtccaaatc aatcaaagtc aaatgcctga tgcccgtccg cgccaaagcc tcgaccgccc
     4921 acgagccgac accgcccacg ccgactacgc agacgtgtgc ctgtgaaaag tgcgccaagg
     4981 cggagtctcc gtagagtctg gcgatgccgc cgaatcgtcg cgaagaggtc aaagcgttgt
     5041 tggacatggt cgaatcctgc aagattgcgt caaatcaaaa agtgaacgaa agccggcaaa
     5101 aaatgcctga ctgcacggaa gcggcatatt atactggaaa gcggtcgggc tgttcggctg
     5161 ttgtataaag cccgacggcg ggtctataat gaaacagcgc aaactgtccc tgcgcccatc
     5221 cgacaagagg gcaccgcgag gcggtgcaaa aggagaatac gatatgtaca aacatttggt
     5281 tgttgccgtt gacggcagtg aaacgtccat caatgccctg aaacatgccg ccgagcttgc
     5341 cggggtcaac ggtgcgcgtc tgactttggt acacgttgcc aatcctgccg aatatatggc
     5401 gctcgccccc gaatttttgc aacacgaaag ctacgaagcc gccgccgtcg cgcaaggcaa
     5461 cgaagttttg gatgccgccg agcgcaccgc ccgggaactg ggtgtggaaa ataccgtcaa
     5521 acacctgctg gttgccaata agggtgcgcg tgaaatggcg caggatttgg ttgattatgc
     5581 cgatgaaaac ggtgccgacc tgctggtgtt gggtacgcac ggacgtaccg ggctgatgca
     5641 ccttttaatg ggcagttttg ctgaaacggt aatgcgccaa agccacctgc cgcttttgat
     5701 tatccgcagc aaagccgaag aggcgtagaa attttcagtt tgaaggcctt aaatgtgatt
     5761 tttcatattt aaggcttttt aatttatctg tttttaacgg gagggcagta tgcctgatta
     5821 catccgcacc tcacgcgccg ccgacagcct gcgcgatatc aaaatcaccc ctcatttcct
     5881 gccgcatacg gacggttcgt gccttattga atgcggcaat agcaaagtga tttgtaccgc
     5941 ttccgtggat gaaaatgtcc catcgtttct gcgcggtaaa aaccaaggct gggtaacggc
     6001 ggaatacggg atgctgcctg cgtccaccgc ttcccggatg cgccgcgaag cttcggcggg
     6061 caaacagtcc gggcgcacac aagaaatcca acgccttatc gggcgttcgc tgcgcgcggt
     6121 cgtggatatg gaaaaactcg gcgaacgaca aatcttgatt gactgcgatg tgattcaggc
     6181 ggatggcggt acgcgcacgg cttcgattac cggcgcattt gtggcactgc acattgccgt
     6241 cggaaagctg atttcagacg gcatattgac cgaaaatccc atccgcgagg ccgttgccgc
     6301 cgtatccgca ggcatagtcg gcggagtgcc gcttttagat ttggattatc ctgaagattc
     6361 cggttgcgac agtgatgtaa atatcgttat gaccgcatcg gggaaaatca tcgaaatcca
     6421 aggtacggcg gaaggcgcgc cattcagttt ggaagaattg ggcaagctga tcggactggc
     6481 ggaaaaaggc ataagagagc tggtgcgtta tcagcaaaat gcgttatccg ccgcctaatt
     6541 actgtccgta tgccgtctga aaaccgttca gacggcattt tgtcaaaaat ctgaaagaac
     6601 aggtttttcg ctgaccgctg tcaaacggca ggcgggaaag cgcagtccgt tcttgaactt
     6661 catgtaaaaa atagtaataa tagccgcttt tccagcaaat atgctgcccg accaacccta
     6721 cacaagaaag cacgaaacat ggcgttaatc gtacataaat acggcggcac atccgtaggc
     6781 tcgcccgaac gcattaaaaa cgtggccaaa cgtgtcgcca aagcccgcgc cgaaggacac
     6841 gacatcgtcg tcgtcgtatc cgccatgagc ggtgaaacca accgcctggt cgcgttggcg
     6901 cacgaaatgc aggagcaccc cgatccgcgc gaattggacg tggttttgtc caccggcgaa
     6961 caagtaacca tcggcctttt ggcgatggca ttgaaagaca tcggcgtgga tgccaaaagc
     7021 tacacaggct ggcaggtcgc cctcaaaacc gataccgccc acaccaaagc ccgcatcgaa
     7081 agcattgatg acgaaaaaat gcgtgccgac cttgccgccg gcaaagtcgt catcgtcgcc
     7141 ggcttccaag gcatcagcag tgaaggcgat atttccacgc tcggacgcgg cggttccgac
     7201 acttccgccg ttgcgcttgc cgcagccctc aaagcggacg aatgccaaat ctataccgac
     7261 gtagacggcg tttacacaac cgacccccgc gtcgtacccg aagcgcgccg catggatacg
     7321 gttacatttg aagaaatgat cgaattggca agcctcggct cgaaagtctt gcaaatccgt
     7381 tcagtagaat tcgccggaaa gtacaaagtg cgcctgcgcg tactgagcag cctgcaagac
     7441 ggcggcaacg gcaccttaat tacctttgaa gaggacgaca acatggaaag agctgccgta
     7501 accggtatcg cattcgataa aaaccaagcc cgcatcaacg tgcgcggcgt acccgacaaa
     7561 cccggcgttg cctatcagat tttaggcgcg atcgccgatg ccaacatcga agtcgatatg
     7621 attatccaaa atgtcggcag cgaaggcaca accgattttt cctttaccgt accgcgcggc
     7681 gattacaagc agactttgga aatcctgtcg gaacgccaag acagtatcgg cgcggcttct
     7741 attgatggcg acgacaccgt atgcaaagtc tccgcagtcg gtttgggtat gcgttcgcac
     7801 gtcggtgtag ccgccaaaat cttccgcacg ctcgccgaag agggtatcaa catccaaatg
     7861 atttccacct ccgaaatcaa agtttccgta ttgattgatg aaaaatacat ggaactggca
     7921 accagggtat tgcataaagc ctttaatttg ggctgatgtt tcacggtttg aaacggacaa
     7981 cggcactgcc tttatcgggc agtgccgttt tgtctgccgc ccgaatgttc ggacggcata
     8041 ttttgacttt ggaacaaaaa atatagtgga ttaaatttat agtggattaa
//
LOCUS       DAWFKN010000088         8018 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFKN010000088 DAWFKN010000000
VERSION     DAWFKN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.088"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            289..1416
                     /locus_tag="WGR46_001823"
     CDS             289..1416
                     /locus_tag="WGR46_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0856707.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1446..2323
                     /locus_tag="WGR46_001824"
                     /pseudo
     CDS             1446..2323
                     /locus_tag="WGR46_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            2327..2515
                     /locus_tag="WGR46_001825"
     CDS             2327..2515
                     /locus_tag="WGR46_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0856708.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(2592..4103)
                     /gene="lysS"
                     /locus_tag="WGR46_001826"
     CDS             complement(2592..4103)
                     /gene="lysS"
                     /locus_tag="WGR46_001826"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0856709.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4100..4252)
                     /locus_tag="WGR46_001827"
     CDS             complement(4100..4252)
                     /locus_tag="WGR46_001827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856710.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(4258..5511)
                     /locus_tag="WGR46_001828"
     CDS             complement(4258..5511)
                     /locus_tag="WGR46_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0856711.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            5969..7765
                     /locus_tag="WGR46_001829"
     CDS             5969..7765
                     /locus_tag="WGR46_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0856712.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1999 a         2242 c         2029 g         1748 t
ORIGIN      
        1 tccactaata gtgtatatta agtacgtctg atatacacga taccctacga gggtgtaagc
       61 tttagttcac atttaaaatg acctctttaa acctgtcttt cggcaggttt ctttttaggt
      121 tgtttggaaa tcgtgtgcag acaaggtgta aaataggtaa cagcataaaa taatgcggtt
      181 ttaccgccca tatatttaca aaagccaaat ttttaaacat atatccttga tatatacacg
      241 gcgtaaacat atactggaaa catctttaaa ttttttgaaa ttttaaatat gagcaactgg
      301 aaacccgata ttccctataa cgatttacca cccctgccgc caaaacagga tattgaaagc
      361 aaaaccatcc tgaaacgttg tatagccgcc cgtgcatccc ttgcccgttt aaagcaggcg
      421 gcagaactga taccgaatca agccatgctg attaacaccc ttcctgttat ggaagcccgt
      481 gcaagttcag aaattgaaaa catcgtaacc acaacggata agctgtttca atccctgcaa
      541 atggatacgg aacggcaaga ccctgccacg aaagaagccc tgcaataccg caccgccctg
      601 tttgcaggct atgaatcact gacgagccgc cctttatgca cacaaaccgc catcatggtc
      661 tgcaacgcca tcaagcaccc ctacgaaatg gccatccgca aaacaggcgg cacagcccta
      721 aaaggaggca acagcggaaa tgttgtctat accccgcccg aaggagaaga aaccatacgc
      781 ggcaagctgg caaattggga gcggtttatt cacgaaagcg gcgatttaga cccgcttatc
      841 atcatggcgg cggcacatta ccaatttgaa gccatccatc cgtttacgga cggcaacggg
      901 cggacggggc gcatattgaa cagcctgcta ttgattgaaa aagggctttt ggatttgcct
      961 attttgtatt tgagccgcta catcatcgaa aacagggcgg actattaccg cctgctttta
     1021 ggcgtaaccg aacggcagga ttgggaaagc tggataatct acatcttaga cggcgtagcc
     1081 gacaccgccg attggacggt atcgaaaata gatgcgatac gccgcctgtt cgagcagaca
     1141 cgtcaacaca tacggacaca cgcacaagga atctacacgc acgaactggt aaatcttctg
     1201 tttgagcagc catatacacg cattgccaac ctagaagcgg cagggatagc caaacggcag
     1261 acggcctcta agtacctgaa agagctttca gacataggtg tgctgcaaga aatcgccatt
     1321 ggcagggaca aactattcat tcatccgcgc ctaatggaac tattgcgggg agagggcaac
     1381 agctttacct cattccaatc cctcgttaaa gcatagccaa aataatcaat aatccggagg
     1441 tcaatatggc aagaaggtca aaaacatttg aagaagctgc tgctgaggtt gaggaacgtt
     1501 tcggtcatcg tggcattaag ttggtcgagt ttgagggtac agccaagccg tgtgtaatca
     1561 actgccctaa acatggaaac caaacctgtt cgaggtactc caatatgttc ataggaagta
     1621 gctggggttg cccctcttgt ggtaatgagc aagctgcaaa agccggtata gcgaccctta
     1681 ggaagaatca catagcgtta gaaatgctga aacaggctgt aacaggtatg accaagcaag
     1741 agcgcatcac gacgcaagcc tacaatgaga tgaccaaatc cgtggcaggt tcaaacagca
     1801 tagtccttaa cgatgtccaa ggcgatacga ccatcaacaa ccatcatacg catacgcaca
     1861 accacagcga tgccgatggc aaagcactgt cgatgaggct cacatcccgt cctttgttgt
     1921 cagaccgtca ggcggcggct ttcgcccgta caggcaaact cacgggcagt ttcgacctgt
     1981 ttgcttcggt ggtcgccccc tcgcagtaca cgtttgccgt tgccatgccc gacacgtcca
     2041 tgtcgccggt tatcgaaaag ggagacttgc tggtggtcga gccgcgtatg tgccctgcgg
     2101 acgaagacat cgcgctgatt gaactgtccg acaagcggct ggtcgtcgcg caccttgtta
     2161 tcgatattgc gggcaggatg ctgatttatc agacgggcag gccgtctgaa gcctttgacc
     2221 tgcccgaagg cagcacgatt ttaggtgtgg tgctggagtc aaaaaacggt ttatgtccgc
     2281 cgcacaggca agaaggcgtg ttgattcgga ttaccacccc tgatgtgtgg acggttggta
     2341 cgatttccgc ttccaaaacg tcgtgtacgc gcccgaccgc agcccggaaa tcagccgtat
     2401 gctttcttcg attttggcag gctacgcgtg ggataccgaa aacccgttcg tggcgaaatc
     2461 cgaacaacgc ctgactgcct tgtccgaatg ggtcggtcag ttagaaagcg aataaatccg
     2521 taccgccata caaaatgccg tctgaatcca atcgggttca gacggcattg ccatttcaac
     2581 tgtttttatg actactcggg gcgcatctgc ggaaacagaa tcacgtcgcg gatggtttgc
     2641 gaatcggtca gcaacatgac caagcggtcg atgccgatgc cgcagccgcc ggtcggcggc
     2701 aggccgaatt ccatcgcgcg gatgtagtcg gcatcgtagt gcatcgcttc gtcgtcgccc
     2761 gcgtcttttt gcgccacttg tgccttgaag cgttcggctt ggtcttccgg atcgttcaac
     2821 tcggagtagc cgtttgccag ttcgcggccg acaacgaaaa gctcgaagcg ttcggtcaag
     2881 cccggtttgc tgtcggatgc gcgtgccaac ggggacactt cgacagggta gtcgataatg
     2941 aaagtcgggt tccacaattt gccctcggcg caaccttcaa acagggcaag ttgcaggctg
     3001 ccgatgcccg gagacggagg caggctttcg ccgtgtttga cgatttcttt tttcagccac
     3061 tccgcatcgt tcaactgctc gtcggtgtag tgcggattgt attttttgat ggcatcgaga
     3121 atggtcagac gctcgaacgg gctttccaaa tcgacttctt tgccgttgta agtgatgttt
     3181 gccgtgccgt ttaccgcgcg ggaagcgttg cggatgatgt cttccgccat ttgcatcatg
     3241 cgttcgtagt cggagaaggc ttcgtagaat tcgatcatgg tgaattcagg gttatggcgc
     3301 acagacatac cttcgttgcg gaagctgcgg ttgatttcaa acacgcgttc caaaccgccg
     3361 acaaccaagc gtttcagata cagctcgggg gcgatgcgca ggtagagcgg aatgtccaag
     3421 gcattgtggt gggtaacgaa gggttttgcc gtcgcgccgc cgggaatcgg gtgcatcatc
     3481 ggggtttcga cttcgagata atgctcgccc accataaaat tacgcacgga ttggatgatt
     3541 tggctgcgtt tgataaaggt attgcgcgat tcttcgttgg caatcaaatc gacatagcgt
     3601 tggcggtatt tggtttcctg atcgctcaag cctttatgtt tgtccggcag agggcgcagg
     3661 gatttggaca gcaggcggat gccggacacg cgtacggtca gttcgccctg gttggttttg
     3721 aacaaagtgc cttccgcgcc gacgatgtcg cccaaatccc aatggttgaa gtcgtccaaa
     3781 acttcttggc tcacgccttt gttgttcaga taaagctgga tttgcccgga cacgtcttga
     3841 atagtggcaa agctcgcctt gcccatttga cgcttcagca tcatgcggcc ggccactttg
     3901 acgggaatgc cttgcggatc gagttcttct ttgccgattt cgccgtattg ggcgtgcaaa
     3961 tcggcggcga agctgtcgcg tttgaagtcg ttgggatagg cgttgcgctg ttggcggatg
     4021 ttgtgcagtt tttcgcggcg cagggcgatg atttggtttt cgtccaactg cggctcggtt
     4081 tgcggatggt tttgttcgct catagggttt tccgaaaaaa taaatcaggc gcaatctgtt
     4141 tcagacgacc tgaccgaatc acaaaatttg cgcgtatttt acgcgatgtc ggcatttttt
     4201 tccataaacg cggcaatgcc gtctgaaagc ggtttgcggt ttcagacggc atcgttatca
     4261 tttgaacatt cccgccaaat tcaataagaa caaaacggta aaaccggtca gataaatcaa
     4321 gcctgccaat gcaagggcat tcatacctga tgtgagtttg tgtttttcat cacctttgac
     4381 caaacggtaa ttcagccagg caaacacagg agcggacaca aaagcggcaa tcatcgcaaa
     4441 tttgagcaga ttcgccatta cgccgtcaaa ccaaaaaatc accgccaaac cgctgcccgc
     4501 cacccaaata ttccaggcaa agaattcggc gttgcccgtt ttgtcttttc cgcgcagcag
     4561 gcgcacgggt tcggcaatgg cacgggcata gccgtccacg acggtaatcg tcgtgccgta
     4621 catacaggca aacgcgataa acgccaccag cgggcgcgac cagccgccga tggtaacggc
     4681 gtacatattg atcaattgcc cgatatattt gccgcccgcc atctgtactg cttcgccgtt
     4741 gccgtattgc acaaacgcac ccagtgcaag gaaaaccaaa gccaaaaccg cactggcgat
     4801 ataaccgacg ttgaaatcaa aaatcccgtc gcggtattcg gaaggattga tgcgttgttt
     4861 ttcggttacc cacaaagaat tgatggcgga aatttcaatc ggcgcgggca tccagcccat
     4921 cagcgcgatc aggaagccca aaccggcaag cgtccacggt gtcggctcga taaaatcgga
     4981 ctgcatctgc ataccgcgcg acatagcgat gccggcggcg gcaagcgtgg cgatactcaa
     5041 agtaacgatg atgattttgg aaacgcgatc caaagcgcgg taacgtccgc tcaccaaaat
     5101 aatcaggcag gatgccataa tcaaggcggc aaccgtgccg gcatcaaaca tcagcgaggg
     5161 aatcgccatt ttgacgatgg cggcggttac aatggcgacc gcgcccgcgt taatcgtggc
     5221 ggagaggatg cacaaaatca ggaataccca caaataaacg cggcttttct cggcataacc
     5281 ttcaatcagg ctcttgcccg tgtccagcgt gtaatgcgcg ctgaagcgga aaaacgggta
     5341 tttgaagagg ttggtcagga tgatgatgag cgcgatctgc cagccgtaaa gcgcgcccgc
     5401 ctgcgtcgag gcaatcaggt gcgaaccgcc gaccgccgcc gaagccatca taatccccgg
     5461 tcccaatgcg ttgattttac ttttccaagt cgaaatatgt tgttcggaca taaagtcttc
     5521 cgtattttta accgtgtttc aacacacaga gccgcatatt cggacacagc cctatctatt
     5581 gctccaattt gggcgggatt gcccccaaac aaacccaaat cctaccgtct tcaaaaacag
     5641 gataccgccc ggtagggaaa ttttgatgaa agcgcgtatt gtaacgtaat ccaaatacct
     5701 gccaacacac actattagaa cttcatgctc aaacttgact atattttccg tattacttcc
     5761 aaaaaaatgc ataaaacgat atcttatgcc taaaatttta caacaaacaa ccttacatcg
     5821 cttttttcgc gcaaacacgc accatccgat cagcccgtcc gttttgcagc aggctggcga
     5881 tttgataaga tggttatgtt tttcagacgg catttcagat ttccgtccat gccgtctgaa
     5941 gccgcaaaac ccgattggag gaactgttat gaataccgta tcgaattacc tgtccgcatt
     6001 gcgcgaagcc atgaaggcgc aaggcttgga tgcactcgtc atcccttccg ccgaccccca
     6061 cctgtccgaa tacctgcccg agcattggca ggcgcgccgc gaattatcgg gctttaccgg
     6121 ctcggtcggc acgtttgtcc tgaccaccga tgaagcgggc gtatgggtgg acagccgcta
     6181 ttgggaacaa gccgccaagc agcttgcggg cagcggcatc gaactgcaaa aaagcgggca
     6241 agtgccgccg tataacgaat ggctcgcggc aaacctgccc gaaaacgccg ccgtcggcat
     6301 cccttccgat atggtctcgc tcaccggcaa acgcactttg gcgcaatccc tgaccgccaa
     6361 aaacatccgc atcgaacacc cggacgatct gcttgaccaa gtgtggacaa gccgtcccgc
     6421 tatccccgcc gaaaccgtgt tcgtccacga ccccgcctac gtctccgaaa ccgccgccga
     6481 aaaactcgcc cgcgtgcgcg ccgtgatggc ggaaaaaggc gcggattacc acttggtttc
     6541 ctcgctcgac gacatcgcct ggctgaccaa cctgcgcggc agcgacgtgc ctttcaatcc
     6601 cgttttcgtg tcctatctat tgattggcaa agacaacgcc gtcctgttta ccgaccgatg
     6661 ccgtctgaat gccgaagccg ccgccgcgct gcaaaccgca ggcatcacgg tcgagcctta
     6721 cgcccaagtt gccgacaaac tcgcgcaaat cggcggcgcg ttgcttatcg aaccgaacaa
     6781 aaccgccgtc agcacgcttg tgcgcctgcc cgaaagcgtg cgccttatcg agggaatcaa
     6841 cccgtccacg ctgttcaaat cctgcaaatc cgaagccgac atcgcccgca tccgcgaagc
     6901 gatggaacac gacggcgcgg cgttgtgcgg tttcttcgcc gagtttgaag acatcatcgg
     6961 caacggcggc agcctgaccg aaatcgacgt ggacaccatg ctttatcgcc accgcagcgt
     7021 gcgcccaggc ttcatttcat tgagtttcga caccatcgca ggcttcaacg ccaacggcgc
     7081 actgccgcat tacagcgcaa cgcccgaaag ccacagcacc atcagcggca acgggctttt
     7141 gctcatcgac tccggcgcgc aatacaaagg cggcacgacc gacatcaccc gcgtcgtccc
     7201 tgtcggcaca cccaccgccg aacaaaaacg cgacaacacc ctcgttctca aagcccatat
     7261 cgcgcttgcc gaagccgtgt tccccgaaaa catcccctcg ccgctgattg atgcgatttg
     7321 ccgcaaaccc ctgtggcagg cgcaatgcga ctacggccac ggcaccggac acggcgtagg
     7381 ctatttcctc aacgtccacg aaggcccgca gcgcatcgcc ttcgccgccc ccgccacgcc
     7441 cgaaaccgcc atgaaaaaag gcatggttac ctccatcgaa cccggactct accgcccggg
     7501 aaaatggggc atccgcattg aaaaccttgc cgccaaccaa gccgtcgccg cccctcaaga
     7561 aaccgaattc ggcagcttcc tctgttttga aaccctgacc ctctgcccca tcgacacccg
     7621 cctgatggac accgccctca tgaccgacgg cgaaatcgac tgggtcaacc gctaccacgc
     7681 cgaagtccgc cgccgcctcg agccgctgac cgaaggcgcg gcaaaagcgt ggctgatcaa
     7741 acgcaccgaa ccgctggcgc gttaaacagc acggcgcaaa aaatgccgtc tgaaagccct
     7801 tcagacggca ttggtttccc aaaacatccc gcaccgtttt catcttgccg caagcaaata
     7861 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtacg aatagtacgg
     7921 aaccgattca ctcggtgctt cagcacctga gagaatcgtt ctctttgagc taaggcgcga
     7981 caacgccgta ctggtttttg ttaatccgct atattccg
//
LOCUS       DAWFKN010000089         7892 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFKN010000089 DAWFKN010000000
VERSION     DAWFKN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.089"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            298..1611
                     /locus_tag="WGR46_001830"
     CDS             298..1611
                     /locus_tag="WGR46_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0856713.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1830..2005
                     /locus_tag="WGR46_001831"
                     /pseudo
     CDS             1830..2005
                     /locus_tag="WGR46_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2001..3251
                     /locus_tag="WGR46_001832"
     CDS             2001..3251
                     /locus_tag="WGR46_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0856714.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3286..4779)
                     /gene="rng"
                     /locus_tag="WGR46_001833"
     CDS             complement(3286..4779)
                     /gene="rng"
                     /locus_tag="WGR46_001833"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0856715.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4993..5250
                     /gene="grxC"
                     /locus_tag="WGR46_001834"
     CDS             4993..5250
                     /gene="grxC"
                     /locus_tag="WGR46_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0856716.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5271..5714
                     /gene="secB"
                     /locus_tag="WGR46_001835"
     CDS             5271..5714
                     /gene="secB"
                     /locus_tag="WGR46_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0856717.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5800..7842
                     /gene="recG"
                     /locus_tag="WGR46_001836"
     CDS             5800..7842
                     /gene="recG"
                     /locus_tag="WGR46_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0856718.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGTQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1651 a         2064 c         2337 g         1840 t
ORIGIN      
        1 aatcccgcgt gtacggcacg ccccgcgctc cgcaatgata ggggcggttt cgtttgaatc
       61 ggcgcaccgg gatgaatgcc cccgcgccgt cccgataccg atttgccggt tgttccgtat
      121 cggttgccgc cgtgaaatga cggacggatg caggaatggc ggcaggcgtg aatgggagga
      181 aatgaccgat tgacttgttt caaaataaaa aagtagaatg cagggcttat cccgcgtctg
      241 tgggcggttt tgcctgcacg gtcggttttc tgtttaaaat aaaataaagg agaaatgatg
      301 ctgacgttta tcggactgct gattatcggg gtcatcgtat ggttgctgct gacggaaaaa
      361 gtgtcgccca tcatcgcatt aatcttggtg ccgctgtttg gggcgttgct ggcggggttt
      421 gatgtatccc aattaaaaga attttattcg ggcggcacca aatcggtgat gcagattgtg
      481 attatgttta tgttttccat tttgtttttt ggaatcatga acgatgtggg gctgtttcgt
      541 ccgatgatag gcggtttgat taagctgact cggggtaata tcgtggcagt gagtgtgggg
      601 acggtcttgg tgtcggtggt ggcgcagttg gacggggcgg gtgcgacgac gtttttattg
      661 gtcgtccccg cccttttgcc gctttacaag cgtctgcata tgaatcctta cctgctgttt
      721 ttgctgctaa cttccagtgc gggattgatt aaccttctgc cgtggggcgg gccgactggg
      781 cgggttgcaa gcgtgttggg cgcagatgtg ggcgaattgt ataaaccttt gttgacggtg
      841 caaattatcg gtgtagtgtt tatccttgcg ctgtccctgc ttttgggtgt gcgtgaaaaa
      901 aggcggattg tccgggagtt gggcgcgttg cccgccgtgg cggatttgat aaagccggtg
      961 cctttgtcgg aagaagaaca aaaattggcg cgtccgaaac tgttttggtg gaatgtcctg
     1021 ctgtttttgg cggcgatgag cctgcttttt tcgggcatct tcccgccggg ttatgtattt
     1081 atgctggctg caacggcggc gttgcttttg aattaccgca gcccgcagga acagatggag
     1141 cggatttatg cccacgccgg cggcgcggtg atgatggcgt ccattatttt ggcggcaggt
     1201 acgtttttgg ggattttgaa gggtgcgggg atgttggacg cgatttccaa agacattgtg
     1261 catatcctgc cggacgcgct gctgccttat ctgcatattg ccatcggtgt gttgggcatt
     1321 ccgcttgagt tggttttgag tacggacgct tattatttcg gactgtttcc gattgtggag
     1381 cagattacct cgcaggcggg cgtggcgccc gaagcagcag gttatgcgat gttgatcggc
     1441 agtatcgtcg gcacttttgt tacgccgctt tcgccggcct tgtggatggg cttgggtttg
     1501 gcgaaattgt cgatgggcaa acacatccgt tattcgtttt tctgggcgtg gggtttgtcg
     1561 ctggcgatat tggccagttc gatagcggca ggaatcgtgc ctctgccgta aacggcgaag
     1621 ccgtctgaag cctgaatcgt tcctgtggtc tgtcgcaggc tctgcctgcc cgtgtgttgc
     1681 aatcgcgccc caatgccgtc tgaaggcagt tgccggcgcg gtttttttgt gccgcagttt
     1741 tgaaaaatac cggcaagcgt aaaggccgct aaattgcggc taattttggg gtgttagaat
     1801 aggcacggtt tattttgaag ggaaagttga tgcgtgtttt gctggtggaa gacgatgcga
     1861 tgattgcgga ggcggtgtcg gcaagtttga aagacggcgg ctatgcggtg gattgggtca
     1921 aaaacggcgc gcggctgccg ctgcccagcc ttatgacttg atgctgctgg atttgggttt
     1981 gccggggcgg gacggtttgg atgtttgttc cgctggcaat gcttgcaggt atgttttcct
     2041 actacgaaac cttccacgaa acggaagcgt tgcaggacga cctgctccgt caggcggcat
     2101 tgtacgtcgc acccgattct aaacccgaaa ctttgcccga gggcgacggc gatacgcgca
     2161 ttttcgtgca gatgccgcag caggaagacc ctgttgtcag cctgcctgcg catctggcgg
     2221 acggtctgca cacgcttcag gcggacgggg acgacgatta ttaccgcgtc tatatccgca
     2281 cgaccgaaca gggacggatt gccgttatgc aggaaaacga ataccgtgaa gatttggcgg
     2341 cggatgcggc acggcaaagc gtgttgcccc tgctggcggc actgccgctg atgatactgc
     2401 tgaccgtgtg gattacgcac aaagccatgc gccccgtccg caaattgtcg caaagtctcg
     2461 aacaacgccg aatcaatgac ctgtctgctt tgagtgtgga caatattccc agtgaaatca
     2521 gagggttcgt aaccgccatc aacctgcttt tgaaacgtgc ggatgaagac atccgccacc
     2581 gtcagcggtt tgtcgccgac gcggcacacg aattgcgtac gccgatgaca gccctttccc
     2641 ttcaggcgga acggctcaac aatatgtcgc tcccacccga tgcagcgcgg cagtctgccg
     2701 ttttgcagca gagcatcagg cgcaacaaac acctgctcga acagcttttg gcactggcgc
     2761 gttcgcagtc ggatgaaacc cctctgacga aaacgacatt cggtctgcaa agccgtttcc
     2821 gccaagtgtt gcaggaactg atgccgctgg ctttggaaaa acgtcaggac atcggcgtgg
     2881 cggtcggagg cgatgtcgaa gtgtctgccg acgaaacgga aatctatacg ctgattaaaa
     2941 cctttgccga caacgcggta cgttacacgc cgcccgaagg caggatagat ttgggtttca
     3001 cggacgaagg gaaatatctc gccgtgtggg tggaagataa cgggaacggc attcccgaat
     3061 ccgaacgcgc ccgcgtcctc gatccgtttt accgtatttt gggaacggag cagcagggga
     3121 cggggctggg gctgtcgatt gccgacacgc ttgccaaaaa atacggcgga catttggaat
     3181 tgaccgacag ccgacgtttc ggacacggtc tgttgatacg ggcgttgttg gacaaggaaa
     3241 ccctgaaata gacagatgcc gtctgaaggc ttcagacggc attttttaca tcagcacaat
     3301 atcgtattgt tcctgcgtgt aagcggtttc gaccgccaga gaaatcggtt tgccgatgaa
     3361 atctatcagc attgccaagg attgcgattc ttcgtccaaa aacaaatcga tgacgttggg
     3421 ggcggcgagg atgcggaaac tttcggcatc gtaacggcgc gcttcgcgga cgatttcgcg
     3481 ctggatttcg tagcacacgg tttgcggcgt tttcagacgg cctctgcctt ggcaggaagg
     3541 gcagggttcg cagaggactt ggtttaagtt ttcgcgcgag cgtttgcgcg tcagctcgac
     3601 cagccccagg ctggtaaaac cgtgcagggt aacgcgggta cggtcgaagg cgagggcttt
     3661 ggcaagctcc tgcaacacgg cttcgcggtg gctttcctgc gccatatcga tgaagtcgat
     3721 gatgatgatg ccgccgaggt tgcgtagcct caattcgcgg gcgatggtgt ggcaggcttc
     3781 gaggttggtg cggaagatgg tttcgtcgaa gttgcgtgcg ccgacgaagc cgccggtgtt
     3841 cacgtctatc gtggtcatgg cttcggtgga ttcgataatc aggtagctgc cgaagttgag
     3901 gttgacgcgc ggttgcaggg cgcggctgat ttcctgttcg acgttgtggg tttcaaacag
     3961 cgggcgttcg cctttgaaca attctatcct gcccaatgcg ccgtggacgt attgttcggc
     4021 aaaacgcgtc atgcgcccgt ggtttacggt ggaatcgacg aggatttttt gcgtgtcgca
     4081 gccgaccata tcgcgcaata cgcgcaaact caagggcaaa tcctgataaa gcagggtttc
     4141 cggcggccgg attttcgcct gttcttggat gtgttcccac actttggtca ggtagtcgat
     4201 gtcggactgg agctgttcgt cggtggcgtt ttcggcgttg gtgcggatga tgtagccccg
     4261 gcaggcattt tccggcagga gcttgtcgag gcgttcgcgc aggctgctgc gttcggcatc
     4321 gtcttcgatg cgttgggaca cgccgatgtg gtcttcttgc ggaagatgga cgaggaaacg
     4381 ccccgccagc gagatttggg tggaaagccg cgcgcctttg gtgttgatcg ggtctttgat
     4441 gacctgcacc aaaacagact gcccttcaaa cagcatatgt tcgatgcgct gggtttcttc
     4501 ggggttgcgg cgttgttcga ggacatcgac gatgtgtaaa aacgccgcgc gttccaagcc
     4561 gatgtcgata aacgcgctct gcatcccggg cagcacgcgg cgcaccacgc ccagatagat
     4621 attgccgacc aggctgtgtt cgctgttgcg ctcgatgtgc agctcgcaga tattgttttc
     4681 ctccaacacc gctacgcgcg tttcctgcgg cgtgatgttg accaatatcg tttcgggcgg
     4741 gcgcgcgatg tctttgggga tggggagtcc tgacaacatg gtttttcctg aaaatgtaat
     4801 aaaaatattt ttgcacccgt tccgccgcgc agcgcgggtg caaaaatatt tttcacaatg
     4861 cctatcatac tttaaaaaag aaactttgac acattccgca catgccgttt gaacgtgcat
     4921 ctgttccgca cttgccaaac aggggaaatg cccctatatt gattatcatt gcaaaacttt
     4981 cggaaaccca atatgcagac cgttaccatg tacacaggtc cgttttgtcc ctactgcgcg
     5041 atggcgaaaa ggctgctgca cgcggcaggt gtcggacata tcgacgaaat ccgtgtcgat
     5101 gcaagccccg aagcctttgc cgaaatgcag cagctttcgg gacagcgcag cgtgccgcag
     5161 attttcatcg gcgaaacgca cgtcggcgga tttaccgacc tctaccgcct ccagcaggaa
     5221 ggcgggctgg acggactgct gaacccttaa cccccaacta ggaaaacaaa atgagcgaag
     5281 aactgcaacc cgtattcagc atcgagcgac tgtatgtcaa agacttgtct ttggaagtgc
     5341 cgcacgcgcc gcaaatcttt ttggaacagg gcgagcctga agtggatatg cgcgtttcca
     5401 ccggcagcca aaagctggaa gacggctact acaacgtgga cgttaccgta accgttaccg
     5461 ccaagctgga taacgagcgc acgatgtttt tgaacgaagt aacccaaagc ggcatcttcc
     5521 gtttggaaaa catccccgaa gaagatgtgc agctgctgct gggcgtggcg tgtccgaaca
     5581 tcctcttccc ttacgcgcgc gaagccgttt ccggtacggt aacacgtgcc ggcttcccgc
     5641 ccgtcctgct cgcgccgatt aattttgagg cgatttacca acaacagcag gaagccgaag
     5701 ccgccggggc ttgattcctg cctgccgatg ccgtctgaat ccgtttcaga cggtattttt
     5761 gttttcggat aaaataggtg cgttcaaagg aatctgccta tgatgtcgcc cgaaacccag
     5821 aaacagctca aaatcaccga tgtttccgcc aagaagctcg acaaactcaa cctccatacc
     5881 gcgtgggatt tggtgttgca cctgccgctg cgttacgagg acgagacgca cattatgccg
     5941 attaaggacg cgccgattgg cgtgccgtgc caggtcgagg gcgaggttat ccatcaggaa
     6001 gtaacgttca aaccgcgcaa gcagttgatt gtccaaatcg ccgacggttc cggcagcgtc
     6061 ctttttctgc gcttcatcca cttttacgcc agccatcaga aacagacggc ggtcggcaaa
     6121 cgcatccgcg ccgtgggcga aatcaaacac ggcttttacg gcgacgagat gattcatccc
     6181 aaaatccgcg atgccgaggg cggcggtttg gcggaaagcc tcacgccggt ttacccgacc
     6241 gtaaacggtt tgaaccagcc cactttgcgc cgtattattc agacggcgtt ggacgttacg
     6301 ccgctgcacg acacgctgcc cgatgccctg ctgtgccgtc tgaagctgcc acacctcgcc
     6361 gaaagcctgc gtcttttgca ttcgccgccg ccgagtttca cgattcatca gctttcagac
     6421 ggcacgctgc ctgcatggca acggctcaaa ttcgatgaac ttttggcgca acagctttcg
     6481 atgcgcttgg cgcgacagaa gcgtatcggc ggcacggcgg cggcattggg cggcgacggc
     6541 acattgaccc aagccctgcg ccaagccctg ccgtttgccc tgaccgatgc gcaagaaaaa
     6601 gttgtttccg aaatctgccg cgatatggcg caaacctacc cgatgcaccg cctgctgcaa
     6661 ggcgatgtcg gcagcggcaa aaccattgtc gccgccttgt ccgcgctgac cgccatcgaa
     6721 tccggcacgc aggtggctgt aatggcgccc actgaaatcc ttgccgaaca acattttatt
     6781 aaatttaaac aatggctcga acctttgggt attgaagttg tccgcctttt tggcagtttg
     6841 cgtaaaaaag ccaaagacga agccaaagcc aaactcgccg acggcagcgt caaaatcgcc
     6901 atcggcacgc acgccctgtt ttcagacggc gtggcgtttc acaatttggg cttgaccatt
     6961 gtggacgaac agcaccgttt cggcgttgcc caacgcctcg ccctcaaaaa caaagggcgt
     7021 gaagtccatc agctgatgat gtccgccacg cccatcccgc gcacgcttgc gatgagtttt
     7081 ttcgccgatt tggacgtgtc cgtcatcgac gaattgccgc ccgggcgcac cccgattaaa
     7141 acgcgcctcg tcaacaacgt ccgccgcgcc gaagtcgaag gcttcgtcct cggcacttgc
     7201 cgaaaagggc ggcaggcgta ttgggtctgc ccattgattg aagaaagcga aaccctgcaa
     7261 ctgcaaaccg ccgccgaaac cctcgcccgg cttcagacgg cattgcccga actcaatatc
     7321 ggactggtac acgggcgcat gaaggccgcc gaaaaggccg aagtcatggc ggaatttgcc
     7381 gcaggccgtc tgaacgtttt ggttgccacc accgtcatcg aagtcggcgt agatgtgccc
     7441 aatgccgccc tgatggtcat cgaacacgcc gagcgcatgg gcttggcgca gctgcaccaa
     7501 ttacgcggtc gggtcgggcg cggcgcggca gaaagcgtgt gcgtcctcct gtttgccgaa
     7561 cccttgggcg aactcgccaa agcgcggctg aaagtcatct acgaacacac cgacggcttt
     7621 gaaatcgccc gccaagacct caatatccgc ggcccgggcg agtttctcgg cgcgcgccaa
     7681 agcggcgtgc ctatgctgcg tttcgccaaa ctcgaagaag acctgcacct tttggaacag
     7741 gcgcgcgaaa ccgccccgat gctgattgag caaaaccccg aaatcgtcga agcgcatttg
     7801 gcaaggtggc tttccggcag ggaagggtat ttgggcgtgt gagcaaaatg ccgtctgaaa
     7861 tattagaaaa aaaagtgtaa cggaaatctg at
//
LOCUS       DAWFKN010000090         7889 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFKN010000090 DAWFKN010000000
VERSION     DAWFKN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.090"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(214..285)
                     /locus_tag="WGR46_001837"
     tRNA            complement(214..285)
                     /locus_tag="WGR46_001837"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="WGR46_001838"
     CDS             complement(305..751)
                     /locus_tag="WGR46_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0856719.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGR46_001839"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGR46_001839"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0856720.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1746..1934)
                     /locus_tag="WGR46_001840"
     CDS             complement(1746..1934)
                     /locus_tag="WGR46_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856721.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="WGR46_001841"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="WGR46_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0856722.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="WGR46_001842"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="WGR46_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0856723.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2898..4082
                     /gene="ubiM"
                     /locus_tag="WGR46_001843"
     CDS             2898..4082
                     /gene="ubiM"
                     /locus_tag="WGR46_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0856724.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIECSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(4353..4508)
                     /gene="rpmG"
                     /locus_tag="WGR46_001844"
     CDS             complement(4353..4508)
                     /gene="rpmG"
                     /locus_tag="WGR46_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0856725.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(4540..4773)
                     /gene="rpmB"
                     /locus_tag="WGR46_001845"
     CDS             complement(4540..4773)
                     /gene="rpmB"
                     /locus_tag="WGR46_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0856726.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(5023..6549)
                     /gene="farB"
                     /locus_tag="WGR46_001846"
     CDS             complement(5023..6549)
                     /gene="farB"
                     /locus_tag="WGR46_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0856727.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(6573..7727)
                     /gene="farA"
                     /locus_tag="WGR46_001847"
     CDS             complement(6573..7727)
                     /gene="farA"
                     /locus_tag="WGR46_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0856728.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         2121 a         2176 c         1849 g         1743 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 catgcttctc gaccaacggg acaaactgta ccaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggta
      841 acggcacaat cgactgcttt gcgcgcctcg cctatcgaat atgccaaagc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttcggggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgcccgag gcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac agccgccgca ttgccgaaca gattgtttgc cgttgcccgc
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca ggagggtgga agtgtggata
     1501 aactcgacca tcgccgccag cgaatacagt ttctcgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tctcgagcat ggcagactct
     1741 caggtttacg tttccgtcaa acggcagata ccgcttgaaa gctgattcaa atagcgggca
     1801 attatatcag caaagccatc agggtacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgta
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gtatgggcaa agaccacact
     2461 ttgttcgcca aagttgacgg ttatgtcgaa ttcaaaacca aaggcgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcggct ttttatagtg gattaacaaa aatcaggaca aggcgacgaa
     2641 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa
     2701 tccactatac ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca
     2761 tacaccctcc cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac
     2821 agatagttca aacggcattc aaacccttac aaataccgtc tgaaatcatc gacatccgtc
     2881 tatgcaaagg atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg
     2941 cgggtttaag ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaat
     3001 gcagcccact gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc
     3061 atttttcccg cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa
     3121 tctacccttt gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc
     3181 cccaaccgac cgaggcgcgc ggcgagcctg ccgactgttt gggctatctg atttccaacc
     3241 acaatatccg ccgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga
     3301 ccgatacggc cgttaaagaa gtcaaaacat ctgacaacga agcacaagtc attttagaaa
     3361 acggcaaaat cctgaccgcc cgcctgcttt tggcggcaga cagccgtttt tcgcaaaccc
     3421 gccgacaact cggcatttct tcagatatgc acgactacag ccgaaccatg ttcgtctgcc
     3481 gcatgaaaca tactctttcc aaccaacata ccgcttacga atgcttccat tacgggcgca
     3541 ccatcgcact gctgccgctg gaagaacacc tgaccaatac cgtcattacc gtcgataccg
     3601 ataaaatcaa cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac
     3661 agctcaaggg caggctcggc gatatggaat tggtcagcag catccaccat tacccccttg
     3721 tcggtatgat tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg
     3781 tcggtatgca ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt
     3841 tggcaaaact gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt
     3901 tggaaaaata cagcagcaaa catatgctgc atgcccagcc gctttatcac ggaaccaata
     3961 tgatgctgaa actgtttacc aatgagaccg cgccggcaaa actgctgcgc ggtttggtat
     4021 tgcgtgccgg caacaacttc ccgccgctga aaaaactgat taccaaacaa ctgaccggtt
     4081 gaaaactgcc gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt
     4141 aacaaaaatc aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc
     4201 acttggtgct tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag
     4261 attcgcatac ggcagatgtt tcaagcggaa aggaaacacg ataaaaataa caaagcctcc
     4321 gagcagtcgg aggctttgct ttcaaactga aattatttca gtttagtttc tttatacact
     4381 acgtgtttgc gggcaactgg gtcaaatttt ttgatttcca atttgccggg catagtgcgt
     4441 ttgtttttgg tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta
     4501 tcgcgcattg cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg
     4561 caaatcagcc aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga
     4621 aacgcgcagg cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt
     4681 gggcaaaaaa cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg
     4741 gcgtttgccg gtcactttgc aaactcgtgc cataatcagt ctccaaactt ctaaaatagt
     4801 aaaacgcgat ttataccacg aaaaacagtg ttagttcaag tgttttaaag aaaaacggct
     4861 gttttgcgcg gataatcctt ccgattgtcg catctgtgga acgtttgcag gtgcgaaaca
     4921 gatggggcgc gattatagcg gatttcggga actgctgcat tcaaaatgcc gtccgaacat
     4981 atttccagta ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc
     5041 gccgttgtgg aacggcggtt ttgccagcca tatgacgggt atcatgataa tgaataacaa
     5101 gctgcccgcc ataaagattt cgttcgagcc gataataaaa ccctgctggg taatggtatt
     5161 gttgatgatg cctagggttt gaatgtcgga aacgccgtgc tgggacaaat gagcggccgt
     5221 ttcgtgcaat gttgcggaat agggcgtgat gtgttcggca aagcgcgtgt ggtgcaacgc
     5281 ttcgcgccgt tcccacaggg tgctgacgac ggatacgccg acaccgccca tcagcacgcg
     5341 caggaaattc gacaggctgc cggcggcggc aatctgcccg cccttcatgt gcgacagtgt
     5401 gatggtagtc agcggcagga agaacatggc aacgcctact ccctgccaaa actgcggcca
     5461 gatgacgttg ccgatatcca tgtcggcata aaaatcggtg cgccaataga aagtgaaggc
     5521 aaaggtcagg aagctggcgg ttacgagcag gcgcatatcg attttattgc cgaacctgcc
     5581 gattaacgga gacaggaaga cgggcaggat gccgacgggc gcggcggcaa gccccgccca
     5641 cgtggaggta tagcccaggt tggtctgcaa cactaacggc agcagggtca gcgtccccat
     5701 atacaccata aaacccaatg atgtggcaat gatgccgacg gtaaaattcc gatctttaaa
     5761 cagcgataaa tcgacaatcg gatatttttc tcccaattcc caaacaataa aatacgacaa
     5821 gcacaccagt gcgactacgc ccaaggtaat gatttctcca gaggcgaacc agtcgagttc
     5881 cttacccctg tccaacatca tctgtaacgc gccgataccg actaccatca atgtaagccc
     5941 gacatagtcg gtcggcattt taacggtttc cgtttcccga catttcaaat gtttccatgt
     6001 aatccatgcc gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca
     6061 gtttccggaa atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat
     6121 tgcccacaat gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct
     6181 ttgcgacaag ggaatcagcg gcccggcgat aaagccctgc aagatgcgga aaacaaccag
     6241 cgactgaagg ttgggggcaa taccgcacag ccacgatgtg atgacgaaac cgacagcggc
     6301 ggcggtaaac aatttgacct cgccgatacg ttttgccaaa aagcccgtta acggcacgga
     6361 aacggcgttt gccacagcaa aagaagtaat gacccacgtc ccctgcgtgg ttgccgcacc
     6421 gaggttgccg gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat
     6481 aaatacggca agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg
     6541 ataatccatt gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca
     6601 tcgaccgtcc gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc
     6661 ggcgtttttg aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa
     6721 ccgatacgca acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg
     6781 cgctgcacca ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg
     6841 ctgcccgtac ctgccgaaaa acctgccacc ctgccgcgat aaacaatttg tttgccgtac
     6901 aaatcggaca ccagctcggc aggctgtccg attttcatat gccgcaactg cgtctcttta
     6961 aaattagcat ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc
     7021 gccacctgct gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg
     7081 atttgcgtcc gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc
     7141 gccggctgtt cgcgcaaaga aacctgaccg cccaaagccg cacgtgccga agattcttcc
     7201 gccaaagccg ctttgaccgc cgcctgcgcc tgagacactg ccgcacgggc gtgtgccagc
     7261 tcttcggcgg acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgt
     7321 gcgcgtgcca aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca
     7381 ttttgccggc ggttttgccg caccgcctga accagctcgt tttttgcccg ctcgtaagcc
     7441 agcacatcat tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta
     7501 tcgtcgtgca aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt
     7561 ccggcaacat aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaacgccgac
     7621 ccggcggctg cggcggcaag cgcgaacagc agcgtcaatg ccgtcaggcg gcgtttgcgt
     7681 ttggcttgcg tgttttgaag ttttgtttcg tccgtgtgcg tatccataag attgggttcc
     7741 gaatttcctg atttcatata aacaatgttc tttcaatagg aagcagattc tatatccgcg
     7801 caataacgca atcaagtcag tattttagat gcaatattca aaaaacaacg cttcaggcgg
     7861 caaaactgaa aatatatttc gtttatata
//
LOCUS       DAWFKN010000091         7864 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFKN010000091 DAWFKN010000000
VERSION     DAWFKN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.091"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGR46_001848"
     CDS             complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGR46_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0856729.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(590..1381)
                     /locus_tag="WGR46_001849"
     CDS             complement(590..1381)
                     /locus_tag="WGR46_001849"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0856730.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1430..1837)
                     /locus_tag="WGR46_001850"
     CDS             complement(1430..1837)
                     /locus_tag="WGR46_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0856731.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1837..2523)
                     /locus_tag="WGR46_001851"
     CDS             complement(1837..2523)
                     /locus_tag="WGR46_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0856732.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2643..3686
                     /locus_tag="WGR46_001852"
     CDS             2643..3686
                     /locus_tag="WGR46_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0856733.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3849..5075
                     /locus_tag="WGR46_001853"
     CDS             3849..5075
                     /locus_tag="WGR46_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0856734.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5361..7511
                     /gene="yccS"
                     /locus_tag="WGR46_001854"
     CDS             5361..7511
                     /gene="yccS"
                     /locus_tag="WGR46_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0856735.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1926 a         2609 c         1717 g         1612 t
ORIGIN      
        1 ttcggacggc attttgtttg ccgccatcag tcggcaaact gttttttcat cctctctcgg
       61 cgttcttggg actcaacaga taaagtggct gtcgggcgtg ccagcagccg cttcagaccg
      121 ataggctctc ccgtatcggc acagaatcca taatcccctt catcaatatt gcggatggtc
      181 gcctgtattt tactgagaag ttttcgttcc cgatcgcggg tacggagttc caatgcgtac
      241 tcttcttcct gtgtggcacg gtcggcagga tcgggggctg attcgtgttc ttggagatgc
      301 cctgtcgtag cggaagcatt ttcgatgagt tcgtcttgca tttttaccag caattcgcgg
      361 aaaaaagcca aatgctcgtc attcatataa tcgtcttccg gcccgttcca attcaaaata
      421 tcttgttctg tcagctttgc cataatgtat atcctctcgg ttcaccgaaa aaaacaaagc
      481 accacaaagt ggctttattc ctttgatttt tataaggctt attgcctagg gcgtttttga
      541 aaacgcgcat cataacacgt tttggattgg tttttttatt ttctttacat tattgatatt
      601 gccgttccat ttcctgcgaa cggcgcacac aggcacaaac gccctcactt atggcttcgg
      661 cgacacgatg ccgcctgaag gcttccacgg cttcgtgggt tgtcccgcct tttgacgtta
      721 catttttttg aagcttctcg aaatcttcac ccgtctgctc ggcaagggca accgctcctt
      781 taaacgttgc cagactgagc gcgcgtgctt ctgccatatc aaacccttgt cggatggcgg
      841 cattttgcaa tgcgtccagc agataaaaca catatgccgg cccgctgccg ctgataccgg
      901 taatgctgtg catttgcgct tcatcttcca accaaacagt caaaccgact gatttcatga
      961 ttcgatcggc aatcctgcgg tctgtttccg atacttccgc ttcggcatac ataccggata
     1021 cgcccagccc gatttttccg ggtgtattcg gcataacccg gacaatgcgg cgtgttcccc
     1081 cgaggtaacg gctgagcgta tcgaccggca atccggctgc gacagaaagc accaatgcgc
     1141 cgttggtgcg gatatttttg cacgcggctt ccatatcctg cggcttgacg gcaaggatta
     1201 aaacatcgtc ggaatgaagc tccggcaagg tttcagaagt ttcgaccccc aactcttttt
     1261 gcaaacgttc gcgcttttcc gcaccccggt tggcaatatg gatgcggtaa ccgccttgtt
     1321 tgaccaatcc gcccgcaacg gcagccgcca tattgccgcc gccgagaaaa taaacattca
     1381 ttatttctac cctattcaaa attacctgac taaattcatt cccaattttt taccatcgga
     1441 cgcgctcgga cagaccgttg cgccgcaacc cctgcttttt caaacaatca aaaccttcct
     1501 gtttgaacgt ctcaatcaaa ggtttgccgt cacgcgtacg ccaatccaac ccgcaggctt
     1561 caaacttccc tattttcctg taaaagtaag attgttgtgc ggattcatat tcctttgccc
     1621 tgacggaacg attgccgtct gaaagcggta tctcgtagtc ctctaaagac ggcggactct
     1681 cgccgccaat atcccaaaaa tcgatggcgg ccggttttct cggcttacac cagccggaca
     1741 gggacgaaca ctcataccaa cccgccaacc ggcagcctgc aaccgtaaag gcaagcaacg
     1801 cagccgttgc cgtatatttt attgcccgaa tttccatcat ccccttttcc cgaaaatcgc
     1861 gctgccgata cggacgtgtg tcgcaccgca ctcaatggcg gcaggcatat cgtccgacat
     1921 ccccatagac agcacgtccg ccttaacgcc agccgtattg aggtcggcaa gcaacttttg
     1981 catggtctga aactgggttt tcaactccgt ttcactgctg ttggctttgg caacgcacat
     2041 cagtccgcgc acgacaaggt tgggcaactt cgccacttcg accgcaagcg cgaccgcttc
     2101 ttcgggagca acaccgtgct ttgccgcctc gcctgcaata ttcacctcga tacacacctg
     2161 caaaggcggc attgaagaag gacgctgccc actcagccgg acggcggtct ttagacggca
     2221 tacggtatgc acccaatgcg cgcgttcggc gacaaacttg gttttgttgg actgcacatc
     2281 gccgatgacg tgccacacga tgtcggtcaa atccgccaac tcttccgttt tgccgtacca
     2341 ctcctgaata tagttctcgc cgaaatcacg ctgtccggcg gcgtaaactt cgcggatgcc
     2401 gtctgaaggg aaagtcttac cgacggcaat caggctgacg gaatgcggct tccttcccgc
     2461 ctgcagaacc aattttccaa tacggtcgga cacctcccga taacgttctt gcaacaccgt
     2521 catagattat cccctaatta aaaatgatta aacagttgaa acccctccag tcagggcggt
     2581 acaatcaagg ttgttagaac cattccaacc aatcgaaaca ttatactaaa cagagccgca
     2641 ttatgcagat taccgactta ctcgccttcg gcgctaaaaa caaagcatcc gaccttcacc
     2701 tgagttcggg catatcccct atgattcggg ttcacggcga tatgcggcgc atcaaccttc
     2761 ccgaaatgag cgcggaagag gtcggtaata tggtaacttc ggtgatgaac gaccaccagc
     2821 ggaaaatcta ccagcaaaac ttggaagtcg acttctcgtt cgaactgccc aacgtcgccc
     2881 gattccgcgt caacgccttc aacaccggcc gcggtcccgc cgccgtattc cgcaccattc
     2941 ccagcaccgt cttatcgctg gaagaattga aagccccgag cattttccaa aaaatcgcag
     3001 aatcgccgcg cggcatggta ttggttaccg gccctaccgg ttcgggcaaa tcgaccacgc
     3061 ttgccgcgat gatcaactac atcaacgaaa cccagccggc acacatcctg accatcgaag
     3121 acccgatcga attcgtccac caaagcaaaa aatccctgat taaccagcgc gagctgcacc
     3181 agcacaccct cagcttcgcc aacgcgctgc gttccgcatt gcgcgaagac cccgacgtta
     3241 tccttgtcgg cgagatgcgc gacccagaaa ccatcggctt ggcgctgacc gccgccgaaa
     3301 ccggacactt ggttttcggc acgctgcaca cgaccggcgc agcaaaaacc gtcgaccgta
     3361 tcgtggacgt attcccggcg ggggaaaaag aaatggtgcg ctctatgctg tccgaatcgc
     3421 tgaccgccgt catctcccaa aacctgctga aaacgcacga cggcaacggc cgtgtcgcct
     3481 cgcacgaaat cctgattgcc aaccccgccg tccgcaacct catccgcgaa aacaaaatca
     3541 cgcagatcaa ctccgtcctg caaaccgggc aggcgagcgg tatgcagacg atggaccaat
     3601 cgctgcaatc gctggtgcgc caagggctga tcgcaccgga agtcgcacgc agacgcgcgc
     3661 aaaacagcga aagtatgagt ttctgacaca caaccgcctt ccggccatac cggcgggaaa
     3721 acaaggcgca aacacgcggg gcgggacgca gcatcccgcc cggctaccct tccgaacagg
     3781 aagcgtccgc cttcctgttg aaacctgccg ccgcaaactg caaggcttaa accgaaaaga
     3841 agctaacgat gaataccgat aacctgcacg acatcttgga cgaaatggtt caagtgtatt
     3901 ctcaaaaaaa acaaagccga tccgaaaccc cggccgaaat cggcgcacac ttccacccgc
     3961 tgctcgaccg cctgtgcgaa accgcagaag cacaaaacgc gtccgacatc cttatcagca
     4021 aaggattccc gccctcgttg aaaatcaaca gcgcattaac cccgcagccg caaaaggcgc
     4081 tgacgggcga ggaaaccgcc gccatcgccg catcgacgat gaacgccgaa caatcggaaa
     4141 tattccggcg cgacggcgaa atcaactact ccgtccagtc gcgcagcggc acgcgctacc
     4201 gcgccaacgc ctaccacagc caaggcagcg caggtttggt tttgcggcgc atcaaccacg
     4261 tcatcccgca aatgcaggaa ttgggcctgc ccgaaaaact caaagacctc gccgtcgcac
     4321 cgcgcgggct gctgattatc gtcgggccta ccggttcggg caaatccacc acgatggcga
     4381 ctatgctcga acaccgcaac aaaaccctgc ccagccatat cgttaccatc gaagacccga
     4441 ttgagtttat ctacaaaccg cgccgctgca tctttaccca gcgcgaaatc ggcgtcgaca
     4501 ccataaactg gcagacggcg gtacaaaacg ctatgcgcca atcccccgac gtggtctgca
     4561 tcggcgaagt ccgcagcagg gaaagtatgg aatacgcgat gcagctcgcc caaaccggcc
     4621 acctgtgcat ttttacgctc cacgccaaca ccgcgccgca gtcgctcgaa cgcatactca
     4681 acttctaccc caaagaacag cacaaccaaa tactgatcga catcgccctc aacctgaccg
     4741 gcatcatctg ccaacgcctc gccctcaaac aagacaaaac gggcaggacg gcggttgtcg
     4801 acttgctcat caacacgccc gccatccaag acttcatcct gaagggcgac ctgatgaaca
     4861 tcagtaaaat catggaaacc gccaaaaccg acggaatgca gacgatggat caaaaccttt
     4921 tcgaactgta ccgtcacggc atcatcagtt acgaagaagc cctgcgccag tccgtttccg
     4981 ccaacaacct gcgattgcac atccaactgc acaaagaagg caaaacgccc gaactccttt
     5041 acgacagggt caacggtctc aacctcattt cctgatccgc aaaacccaat gccgtctgaa
     5101 aaccgcatcc ccgttttcag acggcatgat tttatccgcc ccattcatgt gctacacttt
     5161 atagtggatt aaatttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     5221 cagcacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
     5281 tttcagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata ttcacttcat
     5341 aacaataaac cggtaaaacc atgaaaaccc cactcctcaa gcctctgctc attacctcgc
     5401 ttcccgtttt cgccagtgtt tttaccgccg cctccatcgt ctggcagcta ggcgaaccca
     5461 agctcgccat gcccttcgta ctcggcatca tcgccggcgg ccttgtcgat ttggacaacc
     5521 gcctgaccgg acggctgaaa aacatcatcg ccaccgtcgc cctgtttacc ctctcctcgc
     5581 tcacggcaca aagtaccctc ggcacagggc tgcccttcat cctcgccatg accctgatga
     5641 ccttcggctt taccatttta ggcgcggtcg ggctgaaata ccgcaccttc gccttcggcg
     5701 cactcgccgt cgccacttac accacactta cctacacccc cgaaacctac tggctgacca
     5761 accccttcat gattttatgc ggcaccgtac tgtacagcac cgccatcatc cttttccaaa
     5821 tcatcctgcc ccaccgcccc gttcaagaaa acgtcgccaa cgcctacgaa gcgctcggca
     5881 gctacctcga agctaaagcc gactttttcg atcccgacga agccgaatgg ataggcaacc
     5941 gccacatcga cctcgccatg agcaacaccg gcgtcatcac cgccttcaac caatgccgtt
     6001 ccgccctgtt ttaccgcctt cgcggcaaac accgccaccc gcgcaccgcc aaaatgctgc
     6061 gttactactt tgccgcccaa gacatacacg aacgcatcag ctccgcccac gtcgattatc
     6121 aggaaatgtc cgaaaaattc aaaaacaccg acatcatctt ccgcatccac cgcctgctcg
     6181 aaatgcaggg acaagcctgc cgcaacaccg cccaagccct gcgcgcaagc aaagactacg
     6241 tttacagcaa acgcctcggc cgcgccatcg aaggctgccg ccagtcgctg cgcctccttt
     6301 cagacagcaa cgacaatccc gacatccgcc acctgcgccg ccttctcgac aacctcggca
     6361 gcgtcgacca gcagttccgc caactccgac acagcgactc ccccgccgaa aacgaccgca
     6421 tgggcgacac ccgcatcgcc gccctcgaaa ccggcagcct caaaaacacc tggcaggcaa
     6481 tccgtccgca gctaaacctc gaatcaggcg tattccgcca tgccgtccgc ctgtccctcg
     6541 tcgttgccgc cgcctgcacc atcgtcgaag ccctcaacct caacctcggc tactggatac
     6601 tactgaccgc ccttttcgtc tgccaaccca actacaccgc caccaaaagc cgcgtccgcc
     6661 agcgcatcgc cggcaccgta ctcggcgtaa tcgtcggctc gctcgtcccc tacttcaccc
     6721 cgtctgtcga aaccaagctt tggatcgtca tcgtcagtac caccctcttt ttcatgaccc
     6781 gcacctacaa atacagtttc tccaccttct tcattaccat tcaagccctg accagcctct
     6841 ccctcgcagg tttggacgta tacgccgcca tgcccgtacg catcatcgac accattatcg
     6901 gcgcatccct tgcctgggcg gcggtcagct acctatggcc agactggaaa tacctcacgc
     6961 tcgaacgcac cgccgccctt gccgtatgca gcaacggtgc ctatctcgaa aaaatcaccg
     7021 aacgcctcaa aagcggcgaa accggcgacg acgtcgaata ccgcgccacc cgccgccgcg
     7081 cccacgaaca caccgccgcc ctcagcagca ccctttccga catgagcagc gaacccgcaa
     7141 aattcgccga cagcctgcaa cccggcttta ccctgctcaa aaccggctac gccctgaccg
     7201 gctacatctc cgccctcggc gcataccgca gcgaaatgca cgaagaatgc agccccgact
     7261 ttaccgcaca gttccacctc gccgccgaac acaccgccca catcttccaa cacctgcccg
     7321 aaaccgaacc cgacgacttt cagacagcac tggatacact gcgcggcgaa ctcgacaccc
     7381 tccgcaccca cagcagcgga acacaaagcc acatcctcct ccaacagctc caactcatcg
     7441 cccggcagct cgaaccctac taccgcgcct accgacaaat tccgcacagg cagccccaaa
     7501 acgcagcctg aaaaagtttc ggcattttgt aagagagggc agattgtcag acaggttgca
     7561 agatagtgga taaagctttg cgcagggtaa atgcgtagca actgaaccgt cattcccacg
     7621 aacctacatc ccgtcattcc cacgaaaaca gaaaaccaaa aacagaaacc taaaatcccg
     7681 tcattcccac gaaagtggga atccagttcg ttcggtttcg cttgttttaa gtttcgggta
     7741 acttccactt cgtcattccc gcgcaggcgg gaatccagtg cgttgagctt cagctattta
     7801 gaataaattt tgaaactcta atcccgtcat tcccacgaac ctgcatcccg tcattcccac
     7861 gaaa
//
LOCUS       DAWFKN010000092         7506 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFKN010000092 DAWFKN010000000
VERSION     DAWFKN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.092"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGR46_001855"
     CDS             complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGR46_001855"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0856736.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1527..1784
                     /locus_tag="WGR46_001856"
     CDS             1527..1784
                     /locus_tag="WGR46_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0856737.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1766..2221
                     /locus_tag="WGR46_001857"
     CDS             1766..2221
                     /locus_tag="WGR46_001857"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0856738.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2333..3694
                     /gene="accC"
                     /locus_tag="WGR46_001858"
     CDS             2333..3694
                     /gene="accC"
                     /locus_tag="WGR46_001858"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0856739.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            3955..4842
                     /gene="prmA"
                     /locus_tag="WGR46_001859"
     CDS             3955..4842
                     /gene="prmA"
                     /locus_tag="WGR46_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0856740.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTATQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            4860..5423
                     /gene="orn"
                     /locus_tag="WGR46_001860"
     CDS             4860..5423
                     /gene="orn"
                     /locus_tag="WGR46_001860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0856741.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDDVLNKMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(5827..7110)
                     /gene="hemL"
                     /locus_tag="WGR46_001861"
     CDS             complement(5827..7110)
                     /gene="hemL"
                     /locus_tag="WGR46_001861"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0856742.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKYGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(7174..7347)
                     /locus_tag="WGR46_001862"
     CDS             complement(7174..7347)
                     /locus_tag="WGR46_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0856743.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT         1816 a         2246 c         1894 g         1550 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa tggcgtatgt
      181 aatatgttta tacgaccaaa acggaatgaa ttttaacgta ttgcgcgtca tcaacaatga
      241 ctgagtttct cgcctctcgc gcctgaatct atgtcatata cagttccttt cattcaaatc
      301 aacaaaagaa tgccgtccga acgtccgttc agacggcact tgtcttcccg caatagactt
      361 ggggctgttc taacgtacca ccccttcgtt ccaccccaaa accatcgcat cgccgtagct
      421 gaagaaacgg tattcgcgtt cgaccgcata acgatacgcg gcgcggatat gacccatacc
      481 cgaaaacgcg ctgacaagca tcagcagcgt cgatttcggc aaatgaaaat tggtaaccag
      541 cctgtcgaca acattaaaac ggtagcccgg cgtgatgaaa atatcggtgt cgccctgccc
      601 cgctttcaga cgacccgtcg cacgcgcggc agattcgagg gcgcgcatgg aagtcgtgcc
      661 gactgcccag actttgttcc cccgggcttt tgccgcctca acggcggcgg cggtttcaga
      721 cggcacttca aaccattcgc tgtgcatttt atgctcttcg attttgtcca cacgcacggg
      781 ttggaatgtc cccgcaccga cgtgtagggt tacttctgcg gttactgcgc ctttgtcttt
      841 caggcggcgc aaaagttctt ccgtaaaatg cagacccgcc gtcggcgcgg cgaccgcgcc
      901 ctgatatttg gcataaacgg tttgataacg gctgtcgtca tccgcatcgg cggcgcgttc
      961 gatataaggc ggcaggggca ggtgtccgtt ctgttccaaa agttcgtaaa cggtttgacc
     1021 gccttcaaaa cgcaggcaga acagttcgcc ctcacgcccg accgtcacgg cgcggatgcc
     1081 gccttcaaac accagcccca taccgggctt gggcgatttg gacgaacgga tgtgcgccag
     1141 tgcggtatgg ttgtccaaca cgcgctcaat cagggcttcg atcctgccgc cgctgtcttt
     1201 ctgcccaaac agccgcgcct tcatgacttt ggtgttgttg aacaccaaaa cgtcgcccgc
     1261 ctcaacataa tccggcaaat cgccgaacac ccggtcttgc agcggcatat cgggcaacgc
     1321 aaccaaaagg cggctgctgc cgcgcacttc gggcggatgc tgggcaatca gcttttcggg
     1381 cagggtaaaa tcaaaatctg aaatatccat ttttacactc tcgttcgggc aagccgccat
     1441 tatacgcact ttagcccttt ttcagacggc atctttgtcc gaaaaaccaa cagattagaa
     1501 taaacactct taacccgggg catcttgtgc gcaaaatcaa acttcctgca catttccccc
     1561 aaaaaccgcc gttttttgat attttactgg acatttaccg acaacttcgg gaaaataaac
     1621 acattctcac ggtcgttttc caccacagga aaaccgtatc cgaacaccat tccgcccggt
     1681 ttgcgccgtt gccgcaagcc ggctgttttc tgaaaaacca acgcaacaac ccgccgaaac
     1741 accggcagcc tttaaaggaa cagaaatgga tttgcgcaaa ttaaaaaaac tgattgattt
     1801 ggttgaagaa tcgggtatcg ccgaaatcga agtaaccgaa ggcgaggaaa aagtccgcat
     1861 cacccgaacc atcgccgccg cacccgttta cgccgcgccc gtacctgccg ccgcgccggc
     1921 cgtaacgcct gccgccgcac ccgttgcggc atccgcgcct gccgccgcac ctgccgcccg
     1981 cgatttgtcc gacgcgcaaa aatcacctat ggtcggcacg ttctaccgcg cacccggccc
     2041 gaatgccgcg ccttttgtcg aagtcggcca acaggttaaa gtcggcgaca cgctgtgcat
     2101 catcgaagcg atgaagctga tgaacgaaat cgaagccgaa aaatccggca cggtcaaaga
     2161 aattttggtc gaaaacggta cgcccgtcga attcggcgaa ccgctcttca tcatcggata
     2221 atcctgtttt cagacggcat aaacttccga tgccgtctga aatgctttcc cccttcagcg
     2281 ttcccgcacc cttttttacg gacgggttgc cggaaccgca ggaaaggtca tcatgctgaa
     2341 aaaagtttta atcgccaacc gaggcgaaat cgcattacgc gtactccgtg cctgccgcga
     2401 aatgggcatt gccaccgtcg ccgtgcattc cgaggccgac aaagacagcc tgcacgtcaa
     2461 actcgccgac gaatccgtgt gcatcggccc tgccgcttcc gcgcaaagct acctcaacgt
     2521 ccccgccatt atcgccgccg ccgaagtaag ctgcgcggac gctgtccacc cgggctacgg
     2581 tttccttgcc gaaaacgccg atttcgccga acaggtcgag cagtccggct ttacctttat
     2641 cggcccgaaa cccgacacca tccgcctgat gggcgacaaa gtatccgcca aacacgccat
     2701 gatcgaagcg ggcgtgcctt gtgttcccgg ctctgacggc gcattgcccg acgacggcga
     2761 agaaatcctc aaaatcgccg ataaagtcgg ttatcctgtc attatcaaag cctctggcgg
     2821 cggcggcggt cgcggtatgc gcgtggtcga gaaaaaagaa gacctcctcc aatctgtcga
     2881 aatgaccaag gcagaagcag gcgcggcatt cggcaacccg atggtttaca tggaacgcta
     2941 tttgcaacgt ccgcgccacg tcgaaatcca agtgattgcc gacgaacacg gcaacgccgt
     3001 ctaccttgcc gaacgcgact gttcgctaca acgccgccac caaaaagtca tcgaggaagc
     3061 accggctccg ttcatcactg aaaaagaacg cgccaaaatc ggcaacgcct gtgccgacgc
     3121 ctgcaaacgc atcggctatc gtggcgcagg tacgtttgaa ttcctgtacg aagacggcga
     3181 atttttcttt atcgagatga atacgcgcgt tcaggttgag catccggtta ccgagctcat
     3241 caccggcgtg gacattgtgc aggagcaact ccgcatcgca tccggcctgc cgctgcaata
     3301 caagcagaaa gacatcaaaa tcgaaggcca cgcgtttgag tgccgtatca acgccgaaga
     3361 cccatacaac ttcatcccca gcccgggccc gattgaaagc tgccacctgc ccggcggctt
     3421 cggtatccgc gtcgacagcc acatttacca aggctaccgc atcccgcctt actacgacag
     3481 cctgatcggc aaaatctgcg tacacggcaa aacgcgtgaa caggcaatgg cgaaaatgcg
     3541 cgtcgcactc gccgagctgg cggtaaccgg catcaaaacc aatacgccgc ttcaccgcga
     3601 cctgttcgcc gatgcgggtt tccaaaaagg cggcgtcagc atccactatt tggaacactg
     3661 gctggaagat cgcaaagcca aacaggacaa gtaaaccgcc gccgatatgc cgtctgaagc
     3721 cgcccgtccg cgttcagacg gcatttccct tgccccgcgc cgtctgaaac cgatttcgat
     3781 atagtggatt aactttaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaaagatt
     3841 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
     3901 cgtcgccttg tcctgattta aattcaatcc actatatttc caagaaagcc cgttatgccc
     3961 taccaacaaa tcaccgtcaa cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg
     4021 atggaacacg gcgcactctc cgccgccatc gaagatgcct acgccggcac gcaaaacgaa
     4081 caggcgattt tcggcgaacc cggtatgccc gccgaacaaa tctggcagca gagcaaagtc
     4141 atcgccctgt tcggcgaaca cgacgaagcc gccgccatca tccaaaccgc cacacaagaa
     4201 tgcgggttaa aagacttggc atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt
     4261 ctcacgcaat cgcaattcga ccccatccgg atttccgacc gcctgtggat taccccctct
     4321 tggcacgaag tccccgaagg cagtgccgtc aacctccgcc tcgaccccgg actcgccttc
     4381 ggcaccggca gccacccgac cacgcgcctc tgcctcaaat ggttggatac gcaactcaaa
     4441 aacggcgaaa gcgtcctcga ctacggctgc ggttcgggca tcctgaccat cgccgccctc
     4501 aaactcggtg caggtttcgc cgtcggcgtg gatattgacg aacaggccgt ccgcgccggc
     4561 aaggacaacg ccgcgcaaaa caacgtcgat gcacaattct tcctgcccga cggtctgcct
     4621 caagggcaat tcgacgtagt tgtcgccaac atcctcgcca accctttgcg tatgcttggc
     4681 gaaatgctcg ccgcccgcac caaacagggc ggacgcatcg tgttgtccgg tttgttggac
     4741 gaacaggccg aagaactcgg cggcatttac agccaatggt tcgacctcga cccggcggaa
     4801 accgaggaag gatgggcgcg attgagcggc gtaaaacgct gaaacggaaa ggaaacaccg
     4861 tgcaggataa aaacaacctc tgctggctcg atatggaaat gacggggctg aatcccgaaa
     4921 ccgaccgcat tatcgaagtc gcgatgatta ttaccgactc ggatttgaat gtgttggcgc
     4981 aatccgaagt ttacgccgtc caccaaagcg acgacgtgct gaacaaaatg gacgaatgga
     5041 acaccgccac acacggcagg acggggctga cacagcgcgt acgcgaatcg tcgcataccg
     5101 aagccgaagt cgaacagaaa ctgctggact ttatgtcgga atgggtaccc ggacgcgcca
     5161 cgccgatgtg cggcaactcc atccaccaag accggcgttt tatggtcaaa tatatgccga
     5221 aactggaaaa ctacttccac taccgcaacc tcgacgtttc cacgctgaaa gaactcgcca
     5281 aacgctggaa tccgcccgtt gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg
     5341 acgacatttt ggagagcatc gaagaaatgc gccactaccg cgaacacttt ctgatttccg
     5401 ccccgagagc cgaagcgcaa taagaaacaa acaatgccgt ctgaaacgca gtttgcattt
     5461 cagacggcat ttttacagca gattgaaatc aaaaatatac acgcccgtca ttcccgcaca
     5521 ggcgggaatc cggaaggtcg ggcctgccgt tattttcaat cattacagaa actgaaaggt
     5581 ctggattccc gcctgcgcgg gaatgacggg cgtgtgcatt cttatagtgg attaacaaaa
     5641 atcaggacaa ggcgacgaag ccgcaaacag tacaaatagt acgaaaccga ttcacttggt
     5701 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
     5761 tttgttaatc cactatactt caatctgcca aacagatcga acagagaaac cctgtccgtc
     5821 aaaacatcat tcagccatcg ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat
     5881 cagctcgggc gtatgcgcgg cggaaacgaa acctgcttca taagcggacg ggccgaaggc
     5941 gacattgcgg tcgagcatcc cgtggaaaaa ctgtttgaag ccttcaatat tggaacgcgc
     6001 catatcggca tagtttcgcg gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac
     6061 gctgtcggcg gtgaactcga tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa
     6121 ctgttcggta cgcgccgtca ggttttcata gaagccttcg cgctggatga tttccagcgt
     6181 tttcaagcct gcggcgacag caatcgggtt gcccgacaag gtgcctgcct gatacacgcc
     6241 gcccagcggg gaaatacatt ccataatgtc tttgcgcccg ccaaacgcgg caagcggcat
     6301 accgccgccg atgactttgc ccatcgtggt caggtcgggc gtgatgccgt gcaaagattg
     6361 cgcgccgccg agcgcgacgc ggaagccggt catcacttcg tcgtaaatca acaccgcgcc
     6421 gtatttttcg gtcaatccgc gcaaggcttt gacaaaggct tcggtcgggc ggacgaggtt
     6481 catattgccg acgaagggtt cgacaatcac gcaggcgatt tcattgccgc tttgagcaaa
     6541 ggcttcttcg agttgggcga tattgttgta ctcgagtacc aaagtgtgtt tggtaaagtc
     6601 ggcaggcaca ccggcggaag acgggttgcc aaacgtcagc agaccgctgc cggctttcac
     6661 cagcaggctg tcggaatgcc cgtggtagca gccttcaaac ttgatgattt tgtcacgccc
     6721 ggtaaaaccg cgtgccagac ggatggcggt catggtcgct tcggtaccgg agctgacgag
     6781 gcgcagccgt tcgacggacg gcatgatttt ggcgatttct tcggcgatga cgatttcgcc
     6841 ctcggtaggc gcgccgaacg acaaaccgcc caacgcggct tcgcgcacgg cttcgatgac
     6901 ttcggggtgc gcgtggccga caatcgcagg cccccacgag ccgacatagt cggtgtagcg
     6961 cgtgccgttt tcatcccaaa catacgcgcc ttcggctttt ttgatgaagc gcggtacgcc
     7021 gccgacgctg ccgaatgcgc ggacggggga attcacgccg ccggggatga tggctttggc
     7081 gcggtcgaat aaaatttcgt tacggttcat atttatcctc aaatgccgtc tgaacggcag
     7141 gtttcgggct tagaagcaga aagtcccatt ttatcatttt tcaggttgcg acaaggattt
     7201 gcctgcttct ttgcggatga cgccaaccgc atcccggatg acggaacgct cgtctttttc
     7261 cactttatgt gtaaagcggt agtcccggac aactccttcc cagtcgtaat ccacacacca
     7321 ctcccaatgc cggcgttctg atttcatata aatgaaattg gtcggcaaaa aattataaat
     7381 cggcaggctg acttcatgat aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc
     7441 ctctacctcc gcccgggtcg ttttgccttt aacaaccgtt tgtgcgattc cctcttccgt
     7501 ctgata
//
LOCUS       DAWFKN010000093         7421 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFKN010000093 DAWFKN010000000
VERSION     DAWFKN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.093"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGR46_001863"
     CDS             complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGR46_001863"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0856744.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAMAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLGNAFKALLLGVPSEAELWEGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2993..3787)
                     /locus_tag="WGR46_001864"
     CDS             complement(2993..3787)
                     /locus_tag="WGR46_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0856745.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLYGINAVRYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4536..5405)
                     /locus_tag="WGR46_001865"
     CDS             complement(4536..5405)
                     /locus_tag="WGR46_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0856746.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYRLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGR46_001866"
     CDS             complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGR46_001866"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0856747.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5995..6234)
                     /locus_tag="WGR46_001867"
     CDS             complement(5995..6234)
                     /locus_tag="WGR46_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0856748.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGR46_001868"
     CDS             complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGR46_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0856749.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSTAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1684 a         1957 c         1904 g         1876 t
ORIGIN      
        1 gtattttaat taaccttaat cggtgcactt ctagcaatat agtggattca caaaaaccag
       61 tacagcgttg cctcgcctta ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
      121 tgatttttgt taatccacta taattaatat gactttgcgg ccgttttgcc attgcgtaat
      181 aaaacgatgg ggaagtgatg ataaaacgtg tgtgtaacta tatcagacgg cattgttttt
      241 ctgtttgacg gcctcaatcc aaaattttgc cgacgatttc gcccacgtct ttcgacaatc
      301 cttcctgcgc ccgaatgcgc tgcaatgctt gtttcaccaa gtttttgcgg tgcggctcga
      361 gcttgttgca gaggttgaac gcctgcacta agcgggcggc gacctgcggg ttaaagcggt
      421 cgatttcgat gactttgtcg gcgatgaagc ggtagccgct gccgtcttct gcgtggaaat
      481 gcgggacgtt gcggctgaag ctgccgataa gcgagcgggc tttgttgggg ttttcgaggc
      541 tgaatttcgg atgctgcaag gcagtttgaa cctgttgcag ggtgtcgctg cggcggcttg
      601 agccgacgag ggcgaaatat ttgtccatca ccagcgcatc atccgaaaac ttgtcggcaa
      661 actgagccag caggcggttg cgcgtatcgc tttcgttgcc gttgacggcg gacaggatgc
      721 cccattcgtg ggtcatgttt tgcgccattt cgccgtattt ttcggcaacg gtttcgatgt
      781 gcgcggggtc ggcgcgcagg acgaaggcgc ggcagacgtt gcgcagcgtg cgccagccgg
      841 cagcttcggg gctgtactcg tagctttggt tttcctgctt cgccgcctga cggttcaatt
      901 cgtgccattt cggcaggaag cggacggcga ttgtatccaa caaggcttcg tgcgcctgat
      961 ggtagcgcaa cgggtcgatg ttttccgtgc cctcccacag ctcggcttca gacggcacgc
     1021 ccaaaagcag ggctttgaag gcgttgccta agaggtcgtc tgaaatgact ttttcgacgg
     1081 cggcaagcag tttttcgtgt ttcggcagtt caataccgtc tgaaagtgcg gcaaggttgg
     1141 cggcaacggc acggcggtag agcgtttgtg cggcttccca gcgcgtgaag gcgtcgctgt
     1201 catgggcgag cagaagcagc aggtcgtcgt cgctgtacgg atagttcaaa tgcaccggcg
     1261 cgctgaaccc gcgcagcagc gagggaacga cggcttcggt tacgctttcg aactggaagg
     1321 tctgttcggc ttcggtcagc agtaacacgg cttcggttgc gcgtttgccc tgataatcga
     1381 atgccatcgc ttcgccgttg cggttcaaca gtccgacttt gacgggaatc atcatcggct
     1441 gcttgtccgc catatcgggc gtgggcggca cggtttgttt aatggtcaac tcgaaaatat
     1501 tgtttttcag acggctttcc gcttccaaaa cgggcgtgcc cgcctggctg taccacaagg
     1561 cgaactggtc gagattgatg ccgttcgcgt ccgccatcgc cgcgcggaaa tcgtcgcagg
     1621 tcacggcctg cccgtcgtgt cgttggaaat agagcttcat gcctttttgg aagccctctt
     1681 cgccgagcaa ggtgtgatac atccgcacca cttccgcgcc tttttcataa acggtcatgg
     1741 tgtagaaatt gttcatctcc tcatagctgg cggggcgcac cggatgggcg gtcgggcctg
     1801 cgtcttcggg gaactggtgc tggcgcagca ggcggatgtt ctcgatgcgg cgcacggcgc
     1861 ggctggcgcg gtcgccggaa aattcttggt cgcggaacac ggtcaaccct tctttcagcg
     1921 aaagctggaa ccaatcgcgg caggtaacgc ggttgcctgt ccagttgtgg aaatactcgt
     1981 gtccgaccac ggattcgatg ccttcaaaat cggtatcggt ggcggtacgg ctgtcggcaa
     2041 ggacgaactt ggtattaaaa atgttcagcc ccttgttttc catcgcaccc atattgaaat
     2101 cgcccacggc gacgaccatg aaaatatcca agtcgtattc caaaccgaag cgcgtttcgt
     2161 cccacttcat cgcgtttttc aaggattcca cggcaaagcc gaccttaggc ttgtccgcct
     2221 cggcagtgta aaactcgatt ttgacgtttc tgccgcccat cgtggtgaaa cagtcttccg
     2281 tgaccgccaa atcgcccgcg accaaagcaa acagatagct cggtttggca aacgggtctt
     2341 cccatttcac ccaatggcgg ccgtctgaac actcgccgcc gtcgattttg ttgccgttgg
     2401 agagcaaaac gggatagcgt tttttgtccg caacgatggt ggtcgtgaat ttggacatca
     2461 catccggacg gtcgatgtag aacgtgattt tgcggaagcc ctccggctcg cattgggtaa
     2521 acagattgcc accggaagca tacagcccca tcagcgattt gttttccgcc ggcaggatct
     2581 cggtttccac ttcgacggtg aagcgttcgg acggcacgtc tgcaatcgtc agagtctcgc
     2641 cttccaacac ataatccgcc gccacgccgt tgattttgac ggacaagagt ttcgccgaac
     2701 cgtccaacac cagcggctcc cctaccctct gcggctcaac cgtcaaacgg gatttcacga
     2761 cggtttgcgg ttcggcaata tcgaaatgca ggtcggtttc gagaatacgg taggcaggcg
     2821 tttggtaatc tttcagataa cggacggttt tgctcatttt tttctttcaa tattgtttgg
     2881 tttgactgga aaaggcttca gacggcatag gcaaaccctg caccgatatg ccgtatgaaa
     2941 ccgaaacatc agtgcggacg caccgtcaaa caaccggttt taatttcaat ctttatttcc
     3001 acgcttggac aaattctgcc caatgcggtt tgtcttctgt ttgggcggaa agatagtttc
     3061 tgatccgctt gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaaa
     3121 aacgcagata catcaaatac gtattcacgg catcggtttc acaataattg cggatttcct
     3181 tcaatttacc ggcatggaat gcctcccaga ccttgctgcc gtccataccc aatttacccg
     3241 gaaaaccgca cagtttcgcc atatcgtcca gcggcacatt cgccctcggc tggtaaagcg
     3301 cgagcaaatc catcaaatcg caatgacgtt ggtggtagcg gctgatgtag ttgttccact
     3361 tgaaatcgcg gctgtcgccg aaatcgccgt cgcccatatc ccaatagcgc acggcgttga
     3421 tgccgtacag caatgaacgg taatgcagga caggcaggtc gaaaccgccg ccgttccaac
     3481 tgaccagttg cggcgtatgt ttttcaatca actcgaaaaa tttggcaatc acgacttctt
     3541 cgccatcgtc catctcgccg attgtaccga catggacttt atcctgcccc caacgcatgc
     3601 agcacgaaat cgccacaatc tgatgaagat gatgctgcat aaaatcccca cccgtctgag
     3661 cacggcgttt ttgctgggca aacagcacca cttcatcgtc gggcagcgag gacggcagct
     3721 cgtacaatgt tcggataccc tgcacatcgg gtacggtttc aatatcgaaa gccaaaatcg
     3781 tggtcatgac agcaccttgt atttaaaaca gacttgcgcc tattgtgtca ttaaaaggcc
     3841 gataaaaaaa gaggcaaccc cccacaggat tgccccaata cctcaaatca gagatttacg
     3901 cttcacaaac aatacaggct ttcgcctgcg gctttacccg cgtagctcaa ctctacgccg
     3961 gcaaactttt gtttcaccgt ttccgatgaa accccgacca atcgcaagac tgaccggaaa
     4021 atcctttcaa acagcatttc ctgcctgtcg tgtaattcca tgtagcgaaa tgtacgccat
     4081 tttctacgct ttgccaagca ttttttacaa tataaatgtc aaaacattaa ttttataaaa
     4141 ttgctgaaaa tattaaatat atggattttt attttttata tttcaataaa tataaattta
     4201 attttgattt atatttaaat ttaagcataa aatgtcaaat attaaaggaa acatgaaagg
     4261 catatattaa atatttattt ataacgctat gtttttaaag aaaattaatt ttaatatatt
     4321 aactagattg tctgcatata ttcatgggtt tgcggtattt cttccaaaac ctgcttcgaa
     4381 tttcccgacc aagtcttaaa aatattgttt ttgagatact taaatagcag cgattatcaa
     4441 atgaaatctg ttcatataat ctgccatttt gcatttaaaa aaacaatcag gagtttcgac
     4501 tcgaaatgcc tgatatgttt tgtaatttta cgtagtcagt aaaaatcggg gctgccttcc
     4561 ggacgggttt taaaacgctt gtgcagccaa aaatactgct cgggatgttc gcgcacgcgt
     4621 tcctcgataa aacggttcat gcgctgcgcg tcggcttgcg catcttcact cggaaaggat
     4681 ttccaagccg ggtagaaatg cagtgtaact gtattgtctg cctcgcggac gggaatggcg
     4741 ggtatcactt ttgcatttgc aagcgcggca atgcggctca atccggtaat cgttgccgtc
     4801 tgaataccga agaaatccac aaaaaccgaa tcgttgcgtc cgaaatcctg atcgggcaga
     4861 tacagaaacg gcgcgctgct tttgcggaac tgtttgacga gggcgcgcag cccttcggtg
     4921 cgcccgataa ggaagacgtt gtgatagcgg ttgcgacctt tcaaaatctg ttcgtccaat
     4981 atcttgtttt tttgatggga atacatactg atcagcggga catcctgatt aagcgcgtac
     5041 accgccatct cgaacgcggt gaagtgcgga tacaggatga tgactttttc ccccaccgcc
     5101 agcgcgtcgt ccaaataatg cttattgcgg taacgcacca gcgatttcaa acgcccggca
     5161 ggcgcgtacc aatataaacc gtattccaac atcagtttcg ccatgtgttt gaaatgctgt
     5221 ttcaacacgg ttttacgctt ttcctcactc cattcgggaa aacattttgc caaattgatt
     5281 tcgccgatac ggcggcgcgg tttgaccaga cggtaggcaa gcaaacccgt caggtcggca
     5341 atcttatgca gcagcgcaaa cggcagaaac tgcaaaacat acagtacaaa aaatataaat
     5401 ttcatctcga tacacatttt cttttcagac ggcaaaatac aaatgccgtc tgaaactatt
     5461 gaaacctgcc gcgcttgacc tgcatccctg aaggattgag tttggcggca agcccgtggt
     5521 tgcgtaaggc gtgggtcagc gcgacggcaa gaccgtccgc cgcatccgcc tgcggcgttc
     5581 ccgaaagccc cagcatctgc accaccatat gctgcacctg ttctttcgcc gccttgcctt
     5641 tgccgactac cgcctgtttg acctgcaagg cggtgtattc cgaaacgggc agcttatggc
     5701 tgaccaatgc cgccaatgcc gcgcccctag cctgacccag catcagcgtc gatgcaggat
     5761 tgacgttgac gaacacctgt tccactgccg cctgttgagg cttgtaaacg gcaacgactt
     5821 cgccgatatg ccgcacaatc acggcaatcc tgtctgccag agacgcatcg gcaggcgttt
     5881 tgatgcagcc ggaggcaaca taaaaatgat cccgccccct gacatcgatg atgccgaaac
     5941 ccgttacgcg gctgcccggg tcaatgccca agatacggac ggttgccgac atattcacaa
     6001 caaaccgtgt tgaatcagtt ttttacgcaa ggtattgcgg ttcagcccca gcatcacgga
     6061 tgctttggac tggttgccgc cgcattgttc catcacgcac accagcagcg gtttttccac
     6121 ctgatgcaat accatatcgt acacgccgca aggttcggta ccgttcaggt ctttgaaata
     6181 ttgttccaaa ttttgtctga tacattggga aatatcggga agggtatggg gcataatcgc
     6241 gctttcaaaa ggataatcgg gtgttcagac ggcattttgg cggtaggcgc acgcccaacg
     6301 gtcggttttt ccggcaagtc tttcaagata acctgcaagc atgtcgtatt gcgccgccgt
     6361 actgtccaag cggttgattt cacgacgtgt ctgttcgccg tcgggcattt cgtcgatgta
     6421 ccagcctatg tgtttgcgtg caatgcgcac accggcggtc tcaccataaa acgcgtgcat
     6481 ggcgcggacg tggttcaaaa tggcggcact gcattctgcc aaactcaagg caggcggtaa
     6541 aacaccgtgt tcggcgtaat gtttcaaatc gcggaagaac cacggtctgc cttgcgcgcc
     6601 gcgccctatc atgatgccgt ctgcggcggt ttgtttaagg acggcttggg ctttttgcgg
     6661 cgaagtaatg tcgccgttga cccaaaccgg gatgttcaga cggcatttgg tttcggcaat
     6721 caggtcgtaa gccgcttcgc ctttgtacat ttgcgtgcgc gtgcgtccgt ggacggcaag
     6781 ggcggcaatg ccgcaatctt cggcgatttt ggcgatgacg ggcaggtttt tgtggtcgtc
     6841 gtgccaaccc aaacgggttt tgagggtaac gggtacgccc gccgccttga ccaccgcttc
     6901 caaaatggcg gcaaccagcg gctcgttctg catcagcgcg ctaccggctt ggacattgca
     6961 gactttttta gcgggacagc ccatgttgat gtcgataagc tgcgccccaa ggctgacgtt
     7021 gtaacgcgcg gcatccgcca tctgctgcgg atcgcttccg gcaatctgca cggcaacaat
     7081 gccgccttca tcggcaaaat cgctgcggtg caaggttttt ctagtatttc tgagcgtcgg
     7141 gtcgctggtc agcatttcgc acaccgccca acctgcgcca aaatctcggc aaagtcggcg
     7201 gaacggtttg tcggtaatgc ccgccatcgg cgcaagtgcg atggggttgt cgataaaata
     7261 gccgccgatg tgcataatgg gtccgcgttt caaaaaagtg cgacattgta cattttttaa
     7321 gcaggatttc caatctccgg acgcgcccgc gattggggcg ggcaccgttt tatggcataa
     7381 tccgcacaca gatttcctgc cccgccactc acaggcgggc a
//
LOCUS       DAWFKN010000094         7360 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFKN010000094 DAWFKN010000000
VERSION     DAWFKN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.094"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WGR46_001869"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGR46_001869"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGR46_001870"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGR46_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0856750.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WGR46_001871"
     CDS             complement(1737..2630)
                     /locus_tag="WGR46_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0856751.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WGR46_001872"
     CDS             2972..3421
                     /locus_tag="WGR46_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0856752.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WGR46_001873"
     CDS             3495..3803
                     /locus_tag="WGR46_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856753.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="WGR46_001874"
     CDS             3919..4761
                     /locus_tag="WGR46_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0856754.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="WGR46_001875"
     CDS             4751..5497
                     /locus_tag="WGR46_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0856755.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="WGR46_001876"
     CDS             5519..6106
                     /locus_tag="WGR46_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0856756.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="WGR46_001877"
     CDS             6112..6291
                     /locus_tag="WGR46_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0856757.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="WGR46_001878"
     CDS             6532..6972
                     /locus_tag="WGR46_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856758.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1886 a         1950 c         1669 g         1855 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcctgtcgga tgcacaccgg acaggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttgccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acagcctaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aagtcgccgt ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtgcg caagtcttta atgcgtgagg agtccaagac gtacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggcggctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgataac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tagaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaactgga tggcacgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgacaatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcggcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggtggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccagcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcacgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc ctttaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgcgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgcggttgc acgtgaggcc gattgcggca ttcatctgga cgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctattggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga ggtcgccaaa attgccgact atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaatctccgc ccaataccaa caccgtttcg cccaagccat
     4801 acgcgagggt gaagccgccg acggactgcc gcaagagcgc ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttt
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctggagataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc gcactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatcgg
     6361 cagttcatcc ataatacacg cttcaaaagc agcatattcc tccatacgga atatataaat
     6421 acgcaaaaca cgaaggctgc atcaatttgc tatatttgct ttatttacct tatttcacag
     6481 acggcgctac ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttat tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaataa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgttaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 caattgtttg atacaaaaaa cacggaaatt tccacacaaa actacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cgggacatta accgaaaaac aatatgaaac cgtatgcgga
     6961 aaaaaactct gattgcaact tatacacaaa cttacccaca aaccttatca taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttctc cgaaaagtta
     7081 tcctcgaagt tatccacatt atttttttaa accggcttcc atccgaaata tagtggatta
     7141 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7201 caacgccgta ctggtttaaa tttaatccac tatataaact cgctatacaa tttcactatc
     7261 caaacgtaaa ttgttccatt gatacacaaa actgcttacc cccataattt tgataaagca
     7321 tttcttacat tcccggctcc gtcccgtaac caacacagcg
//
LOCUS       DAWFKN010000095         7327 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFKN010000095 DAWFKN010000000
VERSION     DAWFKN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.095"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(85..954)
                     /locus_tag="WGR46_001879"
     CDS             complement(85..954)
                     /locus_tag="WGR46_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0856759.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGR46_001880"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGR46_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0856760.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WGR46_001881"
     CDS             complement(1963..3060)
                     /locus_tag="WGR46_001881"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0856761.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGR46_001882"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGR46_001882"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0856762.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAIDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WGR46_001883"
     CDS             complement(4392..5291)
                     /locus_tag="WGR46_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0856763.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WGR46_001884"
     CDS             complement(5395..6048)
                     /locus_tag="WGR46_001884"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0856764.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WGR46_001885"
     CDS             complement(6127..6780)
                     /locus_tag="WGR46_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0856765.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7057..7141
                     /locus_tag="WGR46_001886"
     tRNA            7057..7141
                     /locus_tag="WGR46_001886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Leu,seq:caa)
BASE COUNT         1508 a         1742 c         2153 g         1924 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagtt cgtccgaacc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atgtatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgttccc aaaggcgagg aaaagcgggc ggtgcggctg gtgggcaaca catggtttgg
     3301 gccggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc
     3361 gtggcggatt tttttcgccc attcctgcgg gttttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atctatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgcct ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgctttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctggatcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatga atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttt gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aacaggcgcc gacggtgcag atggcatacg
     6121 gttttatcag agcagggtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggt tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agccgacgcg gatgcacttg aagaagcgac ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggtaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtctgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct
     6961 atttaaaatc aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga
     7021 atttcaaaag ccattataat gacgacttct tcaccagccc aggtggcgaa attggtagac
     7081 gcaggggact caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc
     7141 acaaccgctt ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataagac
     7201 taaatatagt ggattaaatt taaaccagta cggcgttgcc tcgccttagc tcaaagagaa
     7261 cgattctcta aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc
     7321 ggcttcg
//
LOCUS       DAWFKN010000096         7197 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFKN010000096 DAWFKN010000000
VERSION     DAWFKN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.096"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            326..1771
                     /gene="zwf"
                     /locus_tag="WGR46_001887"
     CDS             326..1771
                     /gene="zwf"
                     /locus_tag="WGR46_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0856766.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVKRFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2051..2746
                     /gene="pgl"
                     /locus_tag="WGR46_001888"
     CDS             2051..2746
                     /gene="pgl"
                     /locus_tag="WGR46_001888"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0856767.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            2727..3713
                     /locus_tag="WGR46_001889"
     CDS             2727..3713
                     /locus_tag="WGR46_001889"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0856768.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            3763..4611
                     /gene="hexR"
                     /locus_tag="WGR46_001890"
     CDS             3763..4611
                     /gene="hexR"
                     /locus_tag="WGR46_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0856769.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            4732..6378
                     /gene="pgi"
                     /locus_tag="WGR46_001891"
     CDS             4732..6378
                     /gene="pgi"
                     /locus_tag="WGR46_001891"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0856770.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            6392..6943
                     /locus_tag="WGR46_001892"
     CDS             6392..6943
                     /locus_tag="WGR46_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0856771.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1884 a         1896 c         1900 g         1517 t
ORIGIN      
        1 aaaaggtata ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
       61 tgttacaaaa aaaatcagac atcgaaaaga aatttgattt aaatcgcaga atctttcgat
      121 tgttgtaacg gaatagggga aacctgcccg atttttggac taggtcaaat aaattgtaaa
      181 aatgggaata gacaaaaagt gtagcggtgt gtagtattac tactcattat gccgcctgcc
      241 tttgtttccg atattgcgat gcagcgtgat ggggaaaaca ggtttcagac ggcatcgaaa
      301 aattctttcc tgacgagata atgcgatgag tacacaaaca aattttgatt tggtgttgtt
      361 cggtgcgacc ggcgatttgg caatgcgcaa acttttgccc tgcctgtatc aggcgcacgt
      421 ggccggtttg cttcatcccg aaggtcgtat tttgggtgtg agccgcagcg aattggatac
      481 cgaaggtttt ctggcgaagg tggaaaccag ttccaaaatc catgtgaaag aaaatttttc
      541 cgatgaggct tgggcatcgt ttgtcaaacg tttcgcatat ctcaaagtcg atgttacgca
      601 gccggacgat tttgccgctt tgggcgattt ggtcaaggcg cgcaaagaaa ccgacaatgt
      661 cgtcatctat ctttccaccg cgccgaaatt cttcgcgcaa acatgtgaaa accttgccaa
      721 aatcggtttg aacgccgaca acgtgcgtgt ggttttggaa aaaccgttgg gtacggattt
      781 ggcttcttcc caacaaatca ataccgatgt tgcgcgttat ttcaaagaag ggcaaatcta
      841 ccgtatcgac cattatttag gtaaagaaag cctgcaaaac ctgctggcct tgcgttttgc
      901 caatgtgatg ttcgagccgc tttggaacaa taaatacatc gaaagcgtgc agcttaccat
      961 cgccgagcag ttgggcgtgg aagaacgcgg cgagttttac gacattaccg gcgcgttgcg
     1021 cgatatggtg caaaaccact tgatgcaaat gctgtgcatg actgcgatgg aagcccccgc
     1081 cagcttggat gccgacgcgg tgcgcgatga aaaagtcaaa gtcatcaagt cattgaagcc
     1141 gctgaccgtc gaatctgtca atgaaaatgt cgtgcgcgga caatataccg ccgccaaagg
     1201 catgaacggc tatcttgaag aaatcaacgt tccgcaagac agctttaccg aaacctacgt
     1261 cgccattaaa gccgaaatcg aaaacgaacg ctggaagggc gttcccttct acctgcgtac
     1321 cggcaagcgc atggcgggca aagtggcgga aatcgttttg aacttcaaag atttgaacag
     1381 ccatattttt gaaggcagtc gcaccgcgcc caaccggctc gttatcgagt tgcaaccata
     1441 tgaatccgtg cgcctctata cgcagatgaa aaccccgggg gcaggaaata aggtcgaaac
     1501 cgtgccgctg gcaaccgatt tgggcaaagc attggaaagc cgccgcgcgg aagcttacga
     1561 gcgcctgctg ctggatgtga ttaacggcaa actcgctttg tttaaccgcc gcgacgaact
     1621 tgaagccgcg tgggaatatg tgatgccgat tttggaaaac tggacaaata acaccacgcc
     1681 gccgcacggc tacggcgcac actcgtgggg gcctgaagcc gcgcgcgaac tattggcgcg
     1741 cgacggacac aagtggcacg aagagcagta atacaataat gcgttcagac ggcatggggt
     1801 ttgaaatgcc gtctgaacgt aagtaaagca gtatagtgga ttaacaaaaa tcaggacaag
     1861 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     1921 aaatttaatc cactataaaa agcagtcccg atatttggta tcaaaagcat aaacaactgt
     1981 tcggccgaca tattgctcaa cccgtttcca taacggaata tgccgtctga aataaaaaaa
     2041 ggacacaaat atgtttgttt ggcacgaata cgaaaatgcg gcagaagcgg cgcagtcttt
     2101 ggctgacgca gtggcggatg ctttgcaggg cgcactggac gagaagggcg gtgcggtgtt
     2161 ggcagtttcc ggcggacgtt cgccgattgc atttttcaac gccctgtcgc aaaaagattt
     2221 ggattggaaa aacgtcggca tcaccttggc agatgaacgc atcgtgccga ccgtccacgc
     2281 cgacagcaat accggtttgg tgcgcgaata cctgttgaag aacaaagcgg aagcggcaat
     2341 gtggattcct atggtggaag acggaaaaac tgaaaccgaa ttacatcccg atgctgttgt
     2401 cgattatgca ctgaaacatt acaaacagcc cgatgttttg gttttgggta tgggaaacga
     2461 cgggcatacg gcttcgattt tcccgaaagc tccgcagttt cagacggcaa tcgacggttc
     2521 ggcaggtgtc gcgctggtgc ataccacgcc cgttaccgcg ccgcacgagc gcgtcagtat
     2581 gaccttggat gcgattgccc atacggggca tgtgtttttg gcgatacggg gcgaagagaa
     2641 aaaagccgtg ttcgaccaag ccgcacaagg cgaaaaccgc gaatatccga tcaacctcgt
     2701 tttgaaccat caaggagtga actgccatgt cttctacgcc gaataaacaa gccggatatc
     2761 cccgactggt cgccgatatc ggcgggacga atgcacgctt tgcgctggaa accgcgccgc
     2821 gcgtcattga aaaagccgcc gtgcttccgt gtaaagacta cgatacggtt accgatgcgg
     2881 tgcgtgccta tctgaatcaa agcggtgcaa cagccgtacg gcacgcggca tttgccatcg
     2941 ccaacccgat tttgggcgac tgggtgcaga tgaccaacca ccattgggcg ttttccatcg
     3001 aaaccacccg tcagactttg gggctggaca ccctcatcct tttgaacgac tttaccgcgc
     3061 aggcattggc ggtaacgcag acttcaagca aagacctgat gcaggtaggc gggcaaaagc
     3121 ctgtcgaatt tgcccccaaa gccgttatcg gccccggtac cggcctgggc gtgagcggat
     3181 tggtgcacag ccacgcaggc tgggtggctt tggcgggcga gggcgggcat accagtttcc
     3241 cgccgtttga cgatatggaa gtgctgattt ggcagtacgc caaaaacaaa tacggccatg
     3301 tttccgccga acgctttttg agcggcgcgg gcttgagctt ggtttacgag gctttggctg
     3361 caaaacagaa agccaaaccc gccaaactga tgccgtctga aatcacggaa aaggctttga
     3421 gcggcgcgtc gcctttgtgc cgtcagactt tggacatctt ctgcgccatg ctcggcacgg
     3481 ttgcttccaa cctcgccctg acgctgggcg cgcgcggcgg cgtgtacctg tgcggcggca
     3541 ttattccccg cgtgttggaa tatttcaaaa cttccccgtt ccgcagccgt ttcgagaaca
     3601 agggcaggtt tgaagcatat cttgccgcga ttcccgtgta tgtcgtcttg agcgagtttc
     3661 ccggaatttc cggtgcggct gcggctctcg acaaccattt gagaaacgtt taaccacagc
     3721 ggctccttgc agcggggctg cattatcgaa gggcatatca ttatgttaag caaaatcagc
     3781 gaatcactgg caaacctttc cggtgcggaa cgcaaagtcg ccgaatgtgc attggcggaa
     3841 cccaaatggt tcgtccatgc cgccgttgcc gaaattgccg aacgtgcgtc cgtcagccag
     3901 ccgaccgtca tccgattctg ccgcagcttg ggttataaag ggctgcccga gttcaagctc
     3961 gccttgtccg ccagcatcgg tcatgagggt atgccctatg tccacgaaga actcaacgcc
     4021 gacgacgata tggcaagcgt ggtcgagaaa gtgttgggca atgccgccgc ctcgctgttg
     4081 ggcgaacgcc gcttcctgaa agagtcggag ctggaaaacg ccattgccac gctgatgcac
     4141 gcccgtcgcg tcgagtttta cggtgtcggc aattccggca ttgtggcaca ggacgcgcag
     4201 cataaatttt tccgtttcgg catgtccacc gtcgcctatg tcgatacgca cacgcagctg
     4261 atggcggcat ctgttttgag cgatcaggat gttttggttg ccatttccaa cacgggttcg
     4321 tctatcgaac ttttggatgc ggtcagcatc gccaaagaaa acggcgcgtc tgtcatcgca
     4381 ctgacccgca acgattcgcc tctggcgcaa cttgccgact gcgtgttgag cgttgccaca
     4441 caggaaaatg ccgaactcta cacgcccatg gtttcccgcc tcttgcagct tgccgtcatc
     4501 gacattctcg ccatcggact tgccctgcgc ttgggcgatg ctgccagcct gcaattgcag
     4561 aaaagcaaaa aaagcataca caacaagcac atcgattacg acaaagattg accttcagac
     4621 ggcatcccac aaatgccgtc tgaaatgccg aacaacggtc gtcggcggct tgcggcagtt
     4681 tccggcagcc ttttcatccc acaacaaaaa acctcattca ggagcatata gatgaaacac
     4741 tttcacgact tacccgcatg gtcgaaattg tggaaccact ttgacgacag caaaacattg
     4801 catatgcgcg aaatgttcga gcaagatccg cagcgtgcgg aacgctactg gctgcaagtc
     4861 ggcggactga cgctggacta ctccaaaaac cgcatcaacg acgaaaccat gtcgcttttg
     4921 ttcgagttag cgcgtgaagc aggcgtgccg gagcggatgc ggcagatgtt ccacggcgaa
     4981 aaaatcaata ccaccgaaaa ccgcgccgtc ctgcatgtcg cccttcgcaa ccgcaccaat
     5041 tcgccgattg tggttgacgg tgaagatgtg atgcccaaag tcaaccgcgt tttgcaacgt
     5101 atgggcgaat ttgcacacga agtccgcagc ggaagctggc tgggctatac caaccaagtc
     5161 attaccgacg ttgtcaacat cggcatcggc ggatcggatt tgggtccgct gatgatgtgt
     5221 accgcgctca aacctttcgg tcatccgcgc ctcaatatgc acttcgtctc caacgtggac
     5281 ggctcgcaac tgcgcgacgt attgtccaaa gtccaccccg aaaccacgct gttcatcatc
     5341 gcctccaaaa catttaccac gcaggaaacg ctgaccaacg cgctgaccgc gcgcgaatgg
     5401 tttttgaatc atgcgggcga cgaagaagcc gttgccaaac acttcgccgc cgtttccacc
     5461 aatcaaaaag ccgtcgccga attcggcatc gacaccgcca atatgtttga attttgggat
     5521 tgggtcggcg gtcggtacag cctgtggtcc gccatcggat tgccgattat gctgtatctc
     5581 ggcgaagaaa acttcatcga aatgctcaac ggcgcgcacc tgatggacca acacttcatc
     5641 aacacaccgc tcgagcgcaa cctgcccgtc attctcgccc tcatcggcat ctggtatatc
     5701 aactactacg gcggcggcag ccacgtcatc gcgccttacg accaacattt gcaccgcctg
     5761 cccaaattca tccagcagct cgatatggaa agtaacggca aacaggttac gttggacggc
     5821 aaagcagtcg gacacgaaac ctcgccgatt atctggggcg aaacgggcat taacggccag
     5881 cacgccttct tccaactgct gcaccaaggc acgcacatta cccccatcga cctgattgcc
     5941 tcgcttgaaa aacgcagcaa cctgcccgga caccacgaaa tcctgcttgc caatgtcttc
     6001 gcccaagcag aagcctttat gcgcggcaaa acccccgacg aagtccgcgc cgaactcaaa
     6061 gcgcagggta tggatgaggc gcgcatcgaa gagctggtcc cgcacaaaac cttctccggc
     6121 aaccgcccga ccaacctcat tctcatggac aaggtcaacc cgcgcaatat gggcagcctg
     6181 attgccatgt acgaacacaa aaccttcgta caaggcatca tttggggcat caacagcttc
     6241 gaccagtggg gcgtggaact cggcaaacaa ctggctaaaa ccattttggg cgaactgacc
     6301 ggcgaaaccg ggccgcaaaa gcacgacagt tcgaccgaac gcctgatcaa cctctacctg
     6361 cagaccaacc gcaaataaaa cctgcggaaa aatgccgtct gaacgccgac cgtttcagac
     6421 ggcattttta tcgaacagga aaaccgtcgg taaacttgca gagcgtgtgc aatcccgata
     6481 tgatggtttg cataaattta aacatatatg ttccgcagct atggcactga ttaaagagcc
     6541 gttggacaaa gcgaaacaaa ggaacgaaga acttgaagcg gcagaagaag cggcggcgca
     6601 ggaggcattg ggtcgggagc aggaagtcga ccgcgtatcc gaatgggaag aacgctacaa
     6661 gctgtcgcgc agcgagttcg agcagttctg gaaaggattg cctcaaaccg tacagaataa
     6721 gctgcaagcc tcacagaaaa catggaaaag cgggatggat aaaatctgtg ccaacaatgc
     6781 gaaagctgaa ggtaaaacgc caaacggcat aaaattcagc gaactggcat gcaaaacggc
     6841 gaaaaccgaa gcacgcttgg aagagctgca caaccgtaaa aaagccctta tcgacgaaat
     6901 ggccagggaa gcggacaaga aagaactgtc aaagcggctc tgaacagcgc ggttcaggca
     6961 ctgcccgccg atattgccga aaccgtcatg cccgagtacc gcaactgaca aaacggtttg
     7021 aatgccaaat gcgccgacgg cgacgaatac ggcgtggcac aatctgactg ccgtaccaga
     7081 gaaatcaatg cgaaaaccaa agaaatccaa ggttatctga ttgactgaaa cttggatgcg
     7141 ggaatgtcgg cggcttttgc gtttttgtcg tttttatagt gaattaaatt taaaccg
//
LOCUS       DAWFKN010000097         7158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFKN010000097 DAWFKN010000000
VERSION     DAWFKN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.097"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGR46_001893"
     CDS             complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGR46_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0856772.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1588..1743
                     /locus_tag="WGR46_001894"
     CDS             1588..1743
                     /locus_tag="WGR46_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856773.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1740..2171
                     /locus_tag="WGR46_001895"
     CDS             1740..2171
                     /locus_tag="WGR46_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0856774.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2305..6654
                     /gene="app"
                     /locus_tag="WGR46_001896"
     CDS             2305..6654
                     /gene="app"
                     /locus_tag="WGR46_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0856775.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
     gene            complement(6970..>7158)
                     /locus_tag="WGR46_001897"
     CDS             complement(6970..>7158)
                     /locus_tag="WGR46_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856776.1"
                     /translation="PYWFKFNPLYVVEQSFGTLHRKFRYARAAYFGLSKVSAQSHLKA
                     MCLNLLKAANRLSAPAAA"
BASE COUNT         1880 a         2088 c         1752 g         1438 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaaccgaac ggatcggatt cccgttttta
       61 tgggataacg gaatgttcag ccggacaccg ccacggtgct ttcgatccgt ttattttccg
      121 atacaaaacc tcgaaaaaat cacgccgagc aggtcgtccg ccgtaaactc gcccgtgatt
      181 tcgccgcatg cgacctgcgc caagcgcaag tgttcggcaa acagctcgat ttgatggttg
      241 ccgcacaatg ccgccagcga caattcttcc tgcgctgctt tgagtgcgtt gacgtgccgc
      301 gtccgcgcca aaaacagccc ttcgccctcg ccctgccatc cggcctcgcg caacaacgtc
      361 cgtttcagcg cgtccaagcc gtcgccggtt ttcgccgaca gcgcgatgac ggtttccgcg
      421 cccgtaccga acccgcctgc cgcgtgtgcg tgcaaatcgg atttgctgtg gatttcgatg
      481 cgtttcaact ccggcggcaa cgcgtccaaa atcgcccgtg tcttgtcgtt cacgccttcg
      541 cgcggatcga ccaacaccag cgcgacatcg gcttcggata cggctttgcg gctgcgttcg
      601 atgccgatgc gttcgaccac gtcgtccgtc tcgcgcaaac ctgccgtatc gacaatatgc
      661 accggaacgc cgtcaatcag gatacgttcc ctgaccgcgt cgcgcgtcgt tccggcaata
      721 tcggtaacaa tcgccacttc gtcgcccgcc agcgcgttca acaggctgga tttgccgaca
      781 ttcggcgcgc cgaccaatac gacattcaga ccttcgcgca aaatcgcgcc ctgctgcgcg
      841 ttggcaagca catcatccac ggcgcggcgc aagccgtcca gtttgccgcg tgcgtctgcc
      901 gcttcgagaa aatcaatgtc ttcctcggga aaatctaacg tcgcttcgac cagcatccgc
      961 aaggtaatca agtcttcgac cagaccgtgt atccgccgcg aaaaatcgcc tttgagcgag
     1021 cgcaacgcca gacgcgccgc cgaacggctg gatgcgtcaa tcaaatccgc cacgccttcc
     1081 gcctgtgcca agtccagttt gtcgtttaaa aacgcacgct tcgtaaactc gcccggttcg
     1141 gcaaggcgcg cgcccaattc caaacagcgg ttcagcagca tatccatcac taccggcccg
     1201 ccgtgtccct gaagctcgat aacatcttcg cccgtaaaac ttgccggtgc ggcaaaaaaa
     1261 agcaaaagcc cgctgtcgat tgcctgaccg tccgcgtccg taaaatcagc ataggttgcc
     1321 acgcgcggct tgggcgtttt cccacacaaa gcctctgcca tcggcagcag gtttttcccc
     1381 gatatgcgta tcacgcccac gccgccgcgc ccgggtgcgg tagcgactgc cgcaatcgtt
     1441 ggaacgttat ccgacataaa acccccgaaa attcaaaaca gccgcgatta tagcaaatgc
     1501 cgtctgaagt ccgacggttt ggctttcaga cggtataaaa ccgcaaaaat gcttgataaa
     1561 tccgtccgcc tgacctaata taaccatatg gaaaaacgaa acacatacgc cctcctgctc
     1621 ggtataggct cgctgttggg tctgttccat cccgcaaaaa ccgccatccg ccccaatccc
     1681 gccgacgatc tcaaaaacat cggcggcgat tttcaacgcg ccatagagaa agcgcgaaaa
     1741 tgaccgaaaa cgcacaggac aaggcgcggc aggctgtcga aaccgtcgtc aaatccccgg
     1801 agcttgtcga gcaaatcctg tccgacgagt acgtgcaaat aatgatagcc cggcgtttcc
     1861 attcgggacc gttgccgccg ccgtccgact tggcgcaata caacgacatt atcagcaacg
     1921 gggcagaccg cattatggca atggcggaaa aagaacaagc cgtccggcac gaaaccatac
     1981 ggcaagacca aaccttcaac aggcgcgggc aactgtacgg cttcatcagc gtcatcctga
     2041 tactgctttt tgccgtcttc ctcgtatgga gcggctaccc cgcaaccgcc gcctcccttg
     2101 ccggcggcac agtggttgcc ttggcgggtg ctttcgtgat tggaagaagc cgagaccaag
     2161 gcaaaaatta atgacaaatc ctagggcgtg cttcatatcc gcccgaacgc cgaaccgcac
     2221 atataggcac atcccgcgcg ccgcccggaa gcggaagccg cgccctccca aacaaacccg
     2281 aatcccgtca gataaggaaa aataatgaaa acaaccgaca aacggacaac cgaaacacac
     2341 cgcaaagccc cgaaaaccgg ccgcatccgc ttctcgcctg cttacttagc catatgcctg
     2401 tcgttcggca ttcttcccca agcctgggcg ggacacactt atttcggcat caactaccaa
     2461 tactatcgcg actttgccga aaataaaggc aagtttgcag tcggggcgaa agatattgag
     2521 gtttacaaca aaaaagggga gttggtcggc aaatcaatga caaaagcccc gatgattgat
     2581 ttttctgtgg tgtcgcgtaa cggcgtggcg gcattggtgg gcgatcaata tattgtgagc
     2641 gtggcacata acggcggcta taacaacgtt gattttggtg cggaaggaag aaatcccgat
     2701 cagcaccgtt tttcttacca aattgtgaaa agaaataatt ataagcctga caattcacac
     2761 ccttacaacg gcgattacca tatgccgcgt ttgcataaat ttgtcacaga tgcagaacct
     2821 gtcgaaatga cgagtgacat gagggggaat acctattccg ataaagaaaa atatcccgag
     2881 cgtgtccgca tcggctcagg acaccactat tggcgttatg atgatgacaa acacggcgat
     2941 ttatcctact ccggcgcatg gttaattggc ggcaatacac atatgcaggg ttggggaaat
     3001 aatggcgtag ttagtttgag cggcgatgtg cgccatgcca acgactatgg ccctatgccg
     3061 attgcaggtg cggcaggcga cagcggttcg ccaatgttta tttatgacaa aacaaacaat
     3121 aaatggctgc tcaacggagt tttacaaacc ggctaccctt attccggcag ggaaaacggt
     3181 ttccagctga tacgcaaaga ttggttctac gatgacattt acagaggcga tacacatacc
     3241 gtcttttttg aaccgcgcag taacggacat ttttccttta catccaacaa caacggtacg
     3301 ggtacggtaa cagaaaccaa cgaaaaggtt tccaatccaa agcttaaagt acagacagtc
     3361 cgactgtttg acgaatcttt gaatgaaact gataaagaac cagtttacgc ggcagggggt
     3421 gttaatcagt accgtccaag gttaaacaac ggtgaaaacc tttcttttat cgattacggc
     3481 aacggcaaac tcatcttatc aaacaacatc aaccaaggcg cgggcggttt gtattttgaa
     3541 ggtgatttta cggtctcgcc tgaaaacaac gaaacgtggc aaggcgcggg cgttcatatc
     3601 agtgaagaca gtaccgttac ttggaaagta aacggcgtgg caaacgaccg cctgtccaaa
     3661 atcggcaaag gcacgctgca cgttcaagcc aaaggggaaa accaaggctc gatcagcgtg
     3721 ggcgacggta cagtcatttt ggatcagcag gcagacgata aaggcaaaaa acaagccttt
     3781 agtgaaatcg gcttggtcag cggcaggggt acggtgcaac tgaatgccga taatcagttc
     3841 aaccccgaca aactctattt cggctttcgc ggcggacgtt tggatttaaa cgggcattcg
     3901 ctttcgttcc accgtattca aaataccgat gaaggggcga tgattgtcaa tcataatgcc
     3961 acaacaacat ccaccgttac cattacaggg aatgaaagta ttacacaacc gagtggtaag
     4021 aatatcaata gacttaatta cagcaaagaa attgcctaca acggttggtt tggcgagaaa
     4081 gatacgacca aaacgaacgg gcggctcaac cttgtttacc agcccgccgc agaagaccgc
     4141 accctgctgc tttccggcgg aacaaattta aacggcaaca tcacgcaaac aaacggcaaa
     4201 ctgtttttca gcggcagacc gacaccgcac gcctacaatc atttaggaag cgggtggtca
     4261 aaaatggaag gtatcccaca aggagaaatc gtgtgggaca acgactggat caaccgcacg
     4321 tttaaagcgg aaaatttcca tattcagggc gggcaggcgg tgatttcccg caatgttgcc
     4381 aaagtggaag gcgattggca tttgagcaat cacgcccaag cagtttttgg tgtcgcaccg
     4441 catcaaagcc atacaatctg tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa
     4501 aaaaccatta ccgacgataa agtgattgct tcattgacta agaccgacat cagcggcaat
     4561 gtcagccttg ccgatcacgc tcatttaaat ctcacagggc ttgccacact caacggcaat
     4621 cttagtgcaa atggcgatac acgttataca gtcagccaca acgccaccca aaacggcaac
     4681 cttagcctcg tgggcaatgc ccaagcaaca tttaatcaag ccacattaaa cggcaacaca
     4741 tcggcttcgg gcaatgcttc atttaatcta agcaacaacg ccgcacaaaa cggcagtctg
     4801 acgctttccg acaacgctaa ggcaaacgta agccattccg cactcaacgg caatgtctcc
     4861 ctagccgata aggcagtatt ccattttgaa aacagccgct ttaccggaca actcagcggc
     4921 agcaaggata cagcattaca cttaaaagac agcgaatgga cgctgccgtc aggcacggaa
     4981 ttaggcaatt taaaccttga caacgccacc attacactca attccgccta tcgccacgat
     5041 gctgcaggcg cgcaaaccgg cagtgtgtca gacacgccgc gccgccgttc gcgccgttcc
     5101 ctattatccg ttacaccgcc aacttcggta gaatcccgtt tcaacacgct gacggtaaac
     5161 ggcaaattga acggtcaagg aacattccgc tttatgtcgg aactcttcgg ctaccgaagc
     5221 gacaaattga agctggcgga aagttccgaa ggcacttaca ccttggcggt caacaatacc
     5281 ggcaacgaac ccgtaagcct cgatcaattg acggtagtgg aagggaaaga caacaaaccg
     5341 ctgtccgaaa accttaattt caccctgcaa aacgaacacg tcgatgccgg cgcgtggcgt
     5401 taccaactca tccgcaaaga cggcgagttc cgcctgcata atccggtcaa agaacaagag
     5461 ctttccgaca aactcggcaa ggcagaagcc aaaaaacagg cggaaaaaga caacgcgcaa
     5521 agccttgacg cgctgattgc ggccgggcgc gatgccgccg aaaagacaga aagcgttgcc
     5581 gaaccggccc ggcaggcagg cggggaaaat gtcggcatta tgcaggcgga ggaagagaaa
     5641 aaacgggtgc aggcggataa agacagcgcc ttggcgaaac agcgcgaagc ggaaacccgg
     5701 ccggctacca ccgccttccc ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccgcag
     5761 ccccaaccgc aacctcaacc ccaaccgcag cgcgacctga tcagccgtta tgccaatagc
     5821 ggtttgagtg aattttccgc cacgctcaac agcgttttcg ccgtacagga cgaattggac
     5881 cgcgtgtttg ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa
     5941 cactaccgtt cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt
     6001 atgcagaaaa acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa
     6061 aacaccttcg acgacggcat cggcaactcg gcacggcttg cccacggcgc cgttttcggg
     6121 caatacggca tcggcaggtt cgacatcggc atcagcacgg gcgcgggttt tagcagcggc
     6181 agtctttcag acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta cggcattcag
     6241 gcacgatacc gcgccggttt cggcggattc ggcatcgaac cgtacatcgg cgcaacgcgc
     6301 tatttcgtcc aaaaagcgga ttaccgctac gaaaacgtca atatcgccac ccccggtctt
     6361 gcgttcaacc gctaccgcgc gggcattaag gcagattatt cattcaaacc ggcgcaacac
     6421 atttccatca cgccttattt gagcctgtcc tataccgatg ccgcttcggg caaagtccga
     6481 acacgcgtca ataccgccgt attggctcag gatttcggca aaacccgcag tgcggaatgg
     6541 ggcgtaaacg ccgaaatcaa aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc
     6601 ccgcaactgg aagcgcaaca cagcgcgggc atcaaattag gctaccgctg gtaaccgccg
     6661 gatatgccga aaggggtctg acgatgccac cgtgcgctgt caaacccttt ttctgccgcc
     6721 gccttgtgcc tgctccattg tctgataacc gtcaaattga tttttagccc atgtttggtg
     6781 ggtcggggaa atctatatct tcgtccgtgc cgaaatagtc tgagaccttt gcaaaattcc
     6841 tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc cccaaatacc
     6901 tcctaattct acccaaatac ccccttaatc ctccacggac acccgataat caggcatccg
     6961 ggctgctttt taggcggcag cgggcgcact tagcctgttg gccgctttca aaaggttcaa
     7021 acacatcgcc ttcagatggc tttgtgcact cactttgctc agcccgaaat aggctgcccg
     7081 cgcatagcgg aatttacggt gcagcgtacc gaagctctgt tcgaccacat atagtggatt
     7141 aaatttaaac cagtacgg
//
LOCUS       DAWFKN010000098         7113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFKN010000098 DAWFKN010000000
VERSION     DAWFKN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.098"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            1183..1629
                     /gene="yciA"
                     /locus_tag="WGR46_001898"
     CDS             1183..1629
                     /gene="yciA"
                     /locus_tag="WGR46_001898"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0856777.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(1680..2501)
                     /locus_tag="WGR46_001899"
     CDS             complement(1680..2501)
                     /locus_tag="WGR46_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0856778.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADES
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2620..3078
                     /locus_tag="WGR46_001900"
     CDS             2620..3078
                     /locus_tag="WGR46_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0856779.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(3242..4357)
                     /gene="lst"
                     /locus_tag="WGR46_001901"
     CDS             complement(3242..4357)
                     /gene="lst"
                     /locus_tag="WGR46_001901"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0856780.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            4458..4562
                     /locus_tag="WGR46_001902"
                     /pseudo
     CDS             4458..4562
                     /locus_tag="WGR46_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4790..7015
                     /locus_tag="WGR46_001903"
     CDS             4790..7015
                     /locus_tag="WGR46_001903"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0856781.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALST
                     L"
BASE COUNT         1880 a         2102 c         1596 g         1535 t
ORIGIN      
        1 tgcgctaccg cttctgattc cccgataccg atgccgtctg aaccttcaga cggcattttt
       61 gatttacctg ccgtttacag acgcggggcg ggcgtggaaa tacccgaacc gtcattcccg
      121 acaacaccgt aatcttgaaa cccgtcattc ccgacaatac cgcaatcttg aaacccgtca
      181 ttcccgcgca ggcgggaatc cagacctccg acgcggcggg aatctatcgg aaatgactga
      241 aaccccgaga ttctagattc ccacttccgt gggaatgacg tggtgcaggt ttccgtatgg
      301 atggattcgt cattcccgcg caggcgggaa tccagacccc cgacgcggcg ggaatctatc
      361 ggaaatgact gaaaccccgc gttctagatt cccacttccg tgggaatgac ggttcagttg
      421 cgctccgaca acaccgtaat ctcaaaaccc gtccgacaac accgcaatct tgaaattcgt
      481 cattcccgca caggcgggaa tccagacctg tccgcacaga aacttatcgg ataaaaacag
      541 ttgcccaaac cccgcgttct agattcccac tttcgtggga atgacgtggt gcaggttcca
      601 tatggatgga ttcgtcattc ccgcgcaggc gggaatccaa acccccgacg cggcgggaat
      661 ctatcggaaa tgactgaaac cccgagattc tagattccca ctttcgtggg aatgacggtt
      721 cagttgcgtt ccgacaacac cgtaatcttg aaatccgtcc gacaacaccg taatcttaaa
      781 acccgtcatt cccgcgcagg cgggaatcca gacctccgac gcggcgggaa tctatcggaa
      841 atgactgaac cccccgagat tctagattcc cacttccgtg ggaatgacgt ggtgcaggtt
      901 tccgtatgga tggattcgtc attcccgcgc aggcgggaat ctagacctgt ccgcacagaa
      961 acttatcgga taaaaacagt tgctcaaacc cctcgttcta gattcccact tccgtgggaa
     1021 tgacggttca gttgctacga ccccgcccta caatacgcct tgcgaatctg tcccacgccc
     1081 gcccttgtat gtctcaaatg ggttaaacta cgcacttccc gacttcccgt ttcgggcagg
     1141 ggtgcaggtt cgatttatct ttcaataaaa caaggaaaca ttatgcagca cgaagaaggc
     1201 aaccgccaac gccctcaagg cgaactgctc ctgcgtaccg tcgctatgcc gcgcgatacc
     1261 aatcccaacc aagacatttt cggcggctgg attatgtcgc agatggattt gggcggcggc
     1321 atattggcgg cggaaatcgc gcggggacgc atcgttaccg tcgccgttca ggaaatgaac
     1381 tttatccgcc cggtcaaggt cggcaacgtc gtctgctgct acgggcattg cgtccgcgtg
     1441 ggcaatactt ccctccagct taaagtcgaa gtctgggtga aaactttgat gaacgattgc
     1501 gttaccgaag accgccacct cgtaaccgaa gccgtgttca cttatgtcgc catcgatgcg
     1561 gaaggcaatc cgcgcccgat tccgaaagaa ggcaacccca tattggcggg cttattgcct
     1621 actccgtaaa aatacccgta aaaatgccgt ctgaaactgt cttcagacgg cattccgcat
     1681 catttgaacc aagatctgat gcgggaaaac agcgacatac tctgtttttc aaagtcttcc
     1741 cgcacttccg gcacggcttt ttttatcact ttcattcctg caaaagtctg cgccacgggc
     1801 ataatttcaa tcgtgttcac attcatatgc gccggccggc ggtacagcca caatgcggtt
     1861 tccgcaatat cttcggggcg gataaattcc acaccctcat acacgcctgc tgccttctcg
     1921 tcatcgcctt tgaagcgcac gttggaaaac tccgtattgc cgcacaaacc cggctcgata
     1981 ttggtaacgc ggatgttctt atccgccaac tccgcgcgca aattcaggct gaactggcgc
     2041 acaaacgcct tggtcgcccc gtaaacgttg ctgccggcat aagcataatt gcctgcaatc
     2101 gaacccaaat tcatcacata accgccgccg cgttccacca tttgcggcaa aattttgcgc
     2161 gtcaggaacg tcaaacccaa aacattggtt tgaatcatcg tttcccaatc ttcaaaatcc
     2221 gccttgtccg ccgtgtccag acccaaagcc agcccggcat tgttgatgag gcagtcgatg
     2281 tcggaaaatt catcggggat gccgtttaag gcgttttcca ccgactcgcg acgcgacacg
     2341 tccatttcca aagggtaaaa caaagcaccc gattcatccg ccaaagcctg aagcctgtcc
     2401 gcgcggcgcg ccgcaccgat aacgcggtat cccgccccga caaacgcacg gcacatcgct
     2461 tcgccgaatc ctgccgaagc accggtaatt aaaacagcca ttgtttttcc tttctttctc
     2521 gtttcccaaa cctgtttcag gtatcataac acccttcaga cggcatcgcg ccgactgaaa
     2581 atgacaacaa ccataccacc acgacaggac acattcaata tgaaaaccca aatcagcctt
     2641 gctgctgccg ccatcacact gctcctttcc gcctgcggag gcagcggaat accttcccaa
     2701 cccaaaggcg aaatttccga aaaccgcacc gccgcgttca aatccatgat gcccgaattc
     2761 acacgcatgg gcaaaatggt caaagacgaa gaaccttacg atgtcgaaaa attcaaacag
     2821 gctgccgcgg cgtttgccga aagcagcaag aaaccgttca cactttttga gtccgatccg
     2881 caaggcaacg gccgcgccct gcccgccgtt tggtcggatg gtgcaaaatt tgaagccgaa
     2941 aaaacaaaat tcgccgccgc cgtcgaaaaa ctcaacgccg cagcacaaac cggcaaactg
     3001 gatgaaatca aagccgccta cggcgaaacc ggcgcaagct gcaaatcctg ccacgacagt
     3061 ttccgcgcgc ccgaataaca tccaaaacaa aacaatgccg tctgaagcct tcagacggca
     3121 ttttctttgc catttcagca cacgcaaagc aatcagaaat tatcttcaat tcaaaaaata
     3181 cgccatatcc tgatttttgc cttacttact ccgccatttt ctattttgat ttgctatgcg
     3241 attaatcttt atcgtcaaat gtcaaaatcg ggatgccgga ttgggtaaac agggcataca
     3301 ccggcttgag ccaataatct tccggaaggg aagccggttt caatgcgtaa acgtgcacat
     3361 tgggaaaatc cttcatcgtc aacgccgcgc cgctgaaaaa ggtataaatt tcatacctcg
     3421 tatgcgggtt tttcttaatc tcgcgcaaaa tatagtcttc gatgacatag ggcgaattta
     3481 atgtggttac gccggaaagc ccgtaggttt ggcgggggtg cggtgcgaca tattgtatgt
     3541 tgaagttttt tgccgccttt tccgaaattt ccttcatctc cttgtcgggc gaacccaaaa
     3601 gtatccgcac cgtgccgccc gtttcgtccc ccgccttcag ttcggacgca tcgaacagcg
     3661 gcaggtaagt catcttgcgg cggccgtcgt ccataatgtt tttcaaaccc ttgaatatcg
     3721 tgtaatgctc gtcggaagca ttacgggttt tggcgatgct ccaatctccg atcatcatcc
     3781 gggcaaaatt ccgcttgatc gtcccgttta cagaaaactc atcgcccaaa tagctgctgc
     3841 tttgaattaa attgcctgtc ccgtcgtcaa aggttttgat ttccgcatcc gggtaagtgc
     3901 tcaaaaaggc ggcaatgctg actttttcca aacttgccaa ataaatccgc ttgactttcg
     3961 gcagcagcat cgactttacc ttcagctccg ccatcgtcgg aatgaaatta aacgatttgt
     4021 tcaaaccgta gggcaggtgg aaaaaatacg cccgctccgc cttatccttt atctgcttga
     4081 aataataatc gtatttttca ttcctgtttt cagacatcag caccacataa aaccgctccc
     4141 ccggatgctg cgccataatc ctttccgcca ccttcatctg caatatggta tagcagaaaa
     4201 tcagattgac cggttccccc tcttcattga agagtttgtc cttcagcagg gaaaccgcat
     4261 tcctttcccc atgatttacc cggtcaaatg tataaaatat cccgaaacaa aaaacaatca
     4321 aacacaacac ggtcaaacaa gcctttttca agcccatatc cctaaaactc cattccgaca
     4381 aattgaacat acgccccgag tgtcccggga cggaaaaatg cggaatttta gcagatattc
     4441 cgacagttac cgaatattat agtggtttaa atttaaatca ggacaaggcg acgaagccgc
     4501 agacagtaca agcagtacga caaggcaagg caacgctgta ctggtttaaa tttaaaccac
     4561 tataaaatca atttcaccga aaatatgccg ccgcacatca ggcacagcac ctccgaccgc
     4621 caccaaacgc gggaaaacca tttccccctt cctttcacgc tgccgcgttc aaacacgcca
     4681 agatcgaaca aagcgcgaac aaagccgcca aaatatgcca taatagccgc gcacattccc
     4741 cgcgcaatgt gttccaaaag aaaaaaccaa cctacaagag aataatccta tgactcaaaa
     4801 atccaccatt gtttataccc ataccgacga agcccccgcg ctggcgaccc aatcgctgct
     4861 gccgattgtg caggcttttg cccgccacgc cgatattgat gtcaaaactg ccgacatttc
     4921 cctgtccggc cgtattttgg cggcattccc cgaatatctg accgaagcgc aacgcgtacc
     4981 cgacgcgctt gccgaattgg gcgaactggt aaaacaaccc gatgcaaacg taatcaaact
     5041 gccgaacatc agcgcatccg taccgcaact gaccgccgcg attaaagaat tgcagtctaa
     5101 aggctttgcc gttcccgatt atcctgccga cccgcaaacc gatgaagaaa aagccgtgcg
     5161 cgaacgctac gaccgcatca aaggcagcgc ggtaaaccct gtcctgcgtg aaggcaactc
     5221 cgaccgtcgc gcccccaaag cggtgaaaaa ctttgccaaa aaaaatccgc acagcatggg
     5281 cgcatggaca aaagactcca aaacccacgt tgccaccatg caaagcggcg actttttcca
     5341 taacgaacaa tccgttaccg tacctgaagc gacttccgta tccatcgtgt tcaccgacaa
     5401 acaaggcaat aaaaaagagc tgcgcgcgcc tgttgccctg aaagccggcg aaatcatcga
     5461 cgcaaccgtg atgagcaaaa aagccctgct cgccttcctt gccgaacaag tgaaagacgc
     5521 gaaggcaaaa ggcgtattgt tctcactgca tatgaaagcc acgatgatga aagtgtccga
     5581 cccgattatc ttcggacacg ccgtcaaagt gttcttcgcg cctgtatttg aaaaattcgg
     5641 cggcaaactg gctgccgcag gtgtcaacgt caacaacggt ttcggcaacc tgcttgccaa
     5701 tctggacaaa ctggatgcgg acacccgcac cgccgtcgaa gccgaaatcg ccgccgttta
     5761 cgccgccaac cccgatttgg cgatggtcga ttccgataaa ggcattacca acctgcacgt
     5821 tcccagcgat gtcatcgttg atgcttctat gcctgcgatg attcgtaact ccggccgtat
     5881 gtgggataaa gacggcaaag cgcaagacac caaagccgtg attcccgaca gcagctacgc
     5941 cggcgtttac caagcaacca tcgacttctg ccgcgaacac ggcgcatttg acccgacaac
     6001 catgggtact gtgcccaacg tcggactgat ggcgcaagcg gcggaagaat acggctcgca
     6061 caacaaaacc tttgaaatcg aagccgacgg ccaagtccaa gtcattgatg cggcaggaaa
     6121 agtcctaatg cagcacgacg ttgaagccgg cgacatctgg cgtatgtgcc aaaccaaaga
     6181 cgctccggtt aaagactggg tacaacttgc cgtcaaccgc gcccgtctga gcaacacgcc
     6241 tgccgtgttc tggctcgacg aaaaccgtcc gcacgacaag agcctgctcg ccaaggttaa
     6301 agcctacctt gccgaactgg ataccaatgg cctcgacatc cgcgtcctcg ctcctgaaga
     6361 agccgccaag ttcagcttgg gtcgtctgaa aaacggcgaa gacaccatct ccgtaaccgg
     6421 taatgtcttg cgcgactacc tgaccgactt gttcccaatt ttggaactcg gcacaagcgc
     6481 gaaaatgctg tctatcgttc cattgatgaa cggcggcggt atgtttgaaa ccggcgcggg
     6541 cggttctgca ccgaaacatg ttcaacaatt cctcgaagaa aaccatttgc gctgggactc
     6601 gctgggcgaa ttcctcgcac tcgccgtatc gtttgaacat ttggcgcaaa aaaccggcaa
     6661 tgccaaagcc caagtcctcg ccgacacttt ggatgcagcc accgaaaaac tgctgttgaa
     6721 cgacaaatcg cctaaacgta aagccggcga actcgacaac cgcggcagcc atttctacct
     6781 caccctctac tgggcgcaag aattggcggc gcaagacaaa gatgccgaac tgaaagccgc
     6841 atttacgcca ttggcagccg ctttgaccgc cgacgaagcg aaaatcgttg ccgagctttc
     6901 cgccgtacaa ggcaaagcgg ccgacatcgg cggctactac gccgccaatc ctgaaaaagc
     6961 cgcacaagtg atgcgtccga gcgtaacctt taatcaggca ctaagcacct tataagcaca
     7021 aaagtaaaaa tgccgtctga acatttatcg ttcagacggc attagttatc cctatctgcc
     7081 tgattatgat taaacgccgc acccattgga tat
//
LOCUS       DAWFKN010000099         6913 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFKN010000099 DAWFKN010000000
VERSION     DAWFKN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.099"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(1..617)
                     /locus_tag="WGR46_001904"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="WGR46_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
     gene            complement(1968..2983)
                     /locus_tag="WGR46_001905"
                     /pseudo
     CDS             complement(1968..2983)
                     /locus_tag="WGR46_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            4257..6389
                     /locus_tag="WGR46_001906"
     CDS             4257..6389
                     /locus_tag="WGR46_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0856782.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            6613..>6913
                     /locus_tag="WGR46_001907"
     CDS             6613..>6913
                     /locus_tag="WGR46_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856783.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPD"
BASE COUNT         1868 a         1523 c         1446 g         2076 t
ORIGIN      
        1 ccaatgcccg caagattggc ggcttttgcc tgttcggcag tgagttccac tttgtctttg
       61 aagcggctgt gcagattgtc ggctgctaaa agtaaatccc ccatttttgc ggttgtaccg
      121 tctgttttgg tgtagctgcc ttgctgagcc aaagtgttac cgttgccgag atttttattt
      181 acatctttat aggcgagatc caaagattgg atacccaatt cttcaagggt acgcaattca
      241 ttagcttggg aaatgccgtc ctggttgaga tcctgccata cacgcaggga ttggaatgcg
      301 gcgtctgccg cgttgatgat gttgtcgccg tttgaatcca attcggccaa agccgcgtag
      361 ccgtgtttgg caaaagagcc gtctgccagt ttggtgttgt cgccgaagag ttccgcaccg
      421 ttgtcgatga tgccgttgcc gttcaaatcg cgcacaagca gaccgtcatc ggcagcaacc
      481 caacctgtgg cggtgcggat gccttgattg cggtggtcga acaatgcacc tgcaaagcct
      541 ttggcagcga cagtttctat gccgtcgccg tccaaatcta gggcaagggg gtcgtaaacg
      601 tggtattggc cgtttcgatt aagaaatagg aaatctcttg ctcctttata cccttcccat
      661 gctaaaccac ccaacccatc ttcaatccac ccccctaatt tgtctcgtaa ccactcagga
      721 gttaaaacac cagttccacc acccaataat ccgcctaaga ccgcgccacc aagagcaaac
      781 gcacctacag tcagtgcagc aggcaatccc gctaaaccaa gaccagtaac aataaaacca
      841 gcaacccatg caccaaatgc tcctcctatc tcacccaatg ctagtcctgt acctattttt
      901 gatgaagttt ttccaagatt atcatagtca ttttcattga ttgcttttgt ccatgcagaa
      961 ccgagttgaa ttcctgagat tgcacctgtt gcaaatgaag caaatttcgc agcagaggcg
     1021 gcttttgcag aatatatatt tgctgcatta ttagcggctc tgacggactg actaatttta
     1081 gataaatcat gtttcgtaac attgacttct cgagaaaatt ggctttgcat ttgccttaac
     1141 aaagatattt ctgctactcg tgctttatta tttatattat atttactctg catttttctt
     1201 acaacttgtc tcaactgact ttgtgttttt tgtaattcac gttgtaccga ttttcctaaa
     1261 ctttcatgtt tggctgcata attggtagaa tcagttgaca tttgtgtata aaaattttta
     1321 cgaaaatttt cgtaactcag attaattaca tccgtacttg cagttataaa atcttgtata
     1381 aattcaatag aaactgaatt atcatcagat ttggctttgt tattttccat attgagcctc
     1441 tcttaatttg aaaaaattaa cataaacatt taaaaataaa acaaaattat ataataaaaa
     1501 aaataatgag aatataaagg ataaaaagag agttaagtta tttaatcgga cttgttctct
     1561 ataaatcata aggcaccaaa taagccataa taaatttagt aaataaaaac tgcttatacc
     1621 caactttctt ttcttggaac ctattattat ttcaactctg gatgaaaaaa tagaatttaa
     1681 aaattttttt ctttgatata tcgttaatat ctccctagtt tttcttaaaa aaaatataat
     1741 catatatata ctacacaaca ttggtaataa ataaattaaa agcgaaaggt aattaaattg
     1801 ataattgtaa attatataat aaacaacata cgaagaaaaa attattatag atgatttgct
     1861 atacgaataa aaaaatatcc tacctagccc gaaccttctt ataatttgta ctatgtgatc
     1921 attcatttca atatccattt aagctttcaa ttaattttct atatttttca atggcatgta
     1981 tcatttggtt gatgatcaag ccatgtatgc aaagattgga atatggtatc tgtcgtgcta
     2041 ataatgcccc cgttactgtt taatttgcgc acaggaaaac cgtcatatgc agcaatccag
     2101 cccgtggcgg tgcggatacc ttggctgcgg tggtcgaaca atgcacctgc aaggcctttg
     2161 gtggcaacgg tttctatact gtcgccatct agatctaggg caagggggtc ggttacgtgg
     2221 aatttgtcgc tgcggttaag atttaagaaa tcttcaaacc atctccaagc tttttcagct
     2281 cctaatgatc cccaccaagc tcctgcgcca cctccaatta tagttccaac tactagccaa
     2341 cctgcgccac cagtaagtaa ataagtaaac caaaagcccc aacacccaaa gccgcacccc
     2401 attggaagcc cattagccca cccaaaaaag aagctgatga gagaccaaca tcactccaat
     2461 ttccttcttc atccaaggct ttccaccaag cacttcccat actcaaagtt cccatggcaa
     2521 cagttgcccc ataagtaaac ttacccaaat tcgctgccat aacttttgat tttgaagcat
     2581 tctctaacgc tgttgccgca cgtcttgcat attgctgctt gttatagttg ctagcattca
     2641 atgctttttg caaatcactt tgtacttttc ttgcaacaaa tgcctccttt gaattaccac
     2701 gaaatttact agcagctctt ccaattcctt cttttaagct tttttgtgcc tcttctgctg
     2761 tgcgttggaa ttctcttgcc ttagcagaca gacgctcacc ctcccttaaa aatgctcgtt
     2821 gatctatctt gtgcgaaagg gtcaaatatt cttcgtacat tgagttaatc gtaccaatcg
     2881 ccctaaccac attttcatca gaaaatatgg aaataatagc atccctatac gcacctagcg
     2941 taatattgtt tctattatta gttatagcat tattcgaata cataatagca cctccaaact
     3001 tagaactgaa tacgtttata ttctctgtgt aataatatca aataattaat taatggataa
     3061 gtaagtccga aaaaataaca actaaaataa gcaatatact gattctctcc tttattaatt
     3121 agatatattg aaaaacaaat aaaaaataaa cagaaaacat tcatgtgaaa aagccacctc
     3181 atattcttat ttataagttt aaaataacta ggcattgtat atttaaaaat cggtacatcc
     3241 aacggaaaca aaccacataa aaatttttct ttattcttta ttaaagacat agttttattt
     3301 ttcaaaatta gtccagataa tatactaaca attactatta aaacaataag cacagcgtaa
     3361 ttttttataa aattcattaa aataataacg aaaatactaa atagattaca tactgtaaat
     3421 tgaaatataa aatattcagt atttaatcca ttttcaaccg cgagtttttt aaaatatcct
     3481 tgctcgaaaa aattaaaaaa atccattaat actttcctct caagaaaact aattttagtt
     3541 tagttaagat aaaatgcaat attacctttt ctccataaaa gtatggagct tatatacata
     3601 aattatgtgc gaggtagcct gaaaaatatc caatacaaga tagtaggaga tgtttccctt
     3661 atcactattg gccaataccc aattggcagg cactctattt ccaaaatttt ctcaagaata
     3721 gttaaatatt attaattagt ttaaaaacaa taacatacgt ttgtatttat ccgttttggt
     3781 ctgcctgaaa cagactctat gtccatacac ggacatgtag acagtttcct tgttattttg
     3841 gttgctcgtc attagtgatg atactttact cctctcttaa aaaatcgtca agaactgtta
     3901 tcaatcttta aagtcttttt taaacaaata tatatacgtt caggctgtct gaaacacaaa
     3961 aaaatgatgt tacccgaccg tgacagagtc ggggcagccg gtaccttaac cctgcacctt
     4021 aaccctgtgc cttaacggta tataggaggc acgataggtg cgaggcatcc gcatctatct
     4081 tgattcctaa gccgttttac cttcctgaaa cgagaacttt tccatacttt ctctttacaa
     4141 ttcccatata tatccttaca attcgttctg ctgttttatt tatatatttc tttctactta
     4201 atatattcct tttccagcca gccgttatcc acatccaatc taactatatt ttatttatgt
     4261 ctatcgtatc cgcaccgctc cccgcccttt ccgccctcat catcctcgcc cattaccacg
     4321 gcattgccgc caatcctgct gatatacaac atgaattttg tgcttccgca caaagcgatt
     4381 taaatgaaac acaatggctt ttggccgcca aatctttggg attgaaggca aaggtagtcc
     4441 gccagcctgt caaacgtttg gctatggcga ccttacctgc attggtatgg tgtgatgacg
     4501 gcaaccattt tattttggct aaaacagacg gtgggggtga gcatgcccaa tatctaatac
     4561 aggatttaac tacgaataag tctgcggtat tgtcttttgc cgaattttct aacagatatt
     4621 cgggcaaact gatattggtt gcttcccgcg cttcggtatt gggcagtttg gcaaagtttg
     4681 actttacctg gtttattccg gcggtaatca aataccgccg gttgtttttt gaagtattgg
     4741 tggtgtcggt ggtgttgcag ctgtttgcgc tgattacgcc tctgtttttc caagtggtga
     4801 tggacaaggt gctggtacat cggggattct ctactttgga tgtggtgtcg gtggctttgt
     4861 tggtggtgtc gctgtttgag attgtgttgg gcggtttgcg gacgtatctg tttgcacata
     4921 cgacttcacg tattgatgtg gaattgggcg cgcgtttgtt ccggcatctg ctttccctgc
     4981 ctttatccta tttcgagcac agacgagtgg gtgatacggt ggctcgggtg cgggaattgg
     5041 agcagattcg caatttcttg accggtcagg cgctgacttc ggtgttggat ttggcgtttt
     5101 cgtttatctt tctggcggtg atgtggtatt acagctccac tctgacttgg gtggtattgg
     5161 cttcgttgcc tgcctatgcg ttttggtcgg catttatcag tccgatactg cggacgcgtc
     5221 tgaacgataa gttcgcgcgc aatgcagaca accagtcgtt tttagtagaa agcatcactg
     5281 cggtgggtac ggtaaaggcg atggcggtgg agccgcagat gacgcagcgt tgggacaatc
     5341 agttggcggc ttatgtggct tcgggatttc gggtaacgaa gttggcggtg gtcggccagc
     5401 agggggtgca gctgattcag aagctggtga cggtggcgac gttgtggatt ggcgcacggc
     5461 tggtaattga gggcaagctg acggtggggc agctgattgc gtttaatatg ctctcgggac
     5521 aggtggcggc gcctgttatc cgtttggcgc agttgtggca ggatttccag caggtgggga
     5581 tttcggtggc gcgtttgggg gatattctga atgcgccgac cgagaatgcg tcttcgcatt
     5641 tggctttgcc cgatatccgg ggggagatta cgttcgaaca tgtcgatttc cgctataagg
     5701 cggacggcag gctgattttg caggatttga acctgcggat tcgggcgggg gaagtgctgg
     5761 ggattgtggg acgttcgggg tcgggcaaat ccacactcac caaattggtg cagcgtctgt
     5821 atgtaccgga gcagggacgg gtgttggtgg acggcaacga tttggctttg gccgctcctg
     5881 cctggctgcg gcggcaggtc ggcgtggtct tgcaggagaa tgtgctgctc aaccgcagca
     5941 tacgcgacaa tatcgcgctg acggatacgg gtatgccgct ggaacgcatt atcgaagcag
     6001 ccaaactggc gggcgcacac gagtttatta tggagctgcc ggaaggctac ggcaccgtgg
     6061 tgggcgaaca aggggccggc ttgtcgggcg gacagcggca gcgtattgcg attgcccgcg
     6121 cgttaatcac caatccgcgc attctgattt ttgatgaagc caccagcgcg ctggattatg
     6181 aaagtgaacg agcgattatg cagaacatgc aggccatttg cgccaaccgg acggtgctga
     6241 ttatcgccca ccgtctgtcc actgttaaaa cggcacaccg gatcattgcc atggataaag
     6301 gcaggattgt ggaagcggga acacagcagg aattgctggc gaagccgaac ggatattacc
     6361 gctatctgta tgatttacag aacgggtaga aggcggggga tgggcgtgta ggctgctttt
     6421 ttcgtaacgt tctgcgtttt cagaccgtcg tttactgggt ttactgactg aaatgaaacc
     6481 aaagtgcagg ctgccggaaa gactttttct gtctttttag gcagcctgca cttctttttc
     6541 actcatctag agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt
     6601 aggggatttt ccatgagcac cttctttcaa caaaccgcgc aagccatgat tgctaaacac
     6661 atcgaccgct tcccgctatt gaagttggac caggtgattg attggcagcc gatcgaacaa
     6721 tacctgaatc gtcaaagaac ccgttacctt agagaccacc gcggccgtcc tgcctatccc
     6781 ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctctc cgatcccgaa
     6841 ctcgaacaca gcctcatcac ccgtatcgac ttcaacctgt tttgccgctt tgacgaactg
     6901 agcatccctg att
//
LOCUS       DAWFKN010000100         6732 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFKN010000100 DAWFKN010000000
VERSION     DAWFKN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.100"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..131)
                     /locus_tag="WGR46_001908"
     CDS             complement(<1..131)
                     /locus_tag="WGR46_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856784.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     "
     gene            198..500
                     /locus_tag="WGR46_001909"
     CDS             198..500
                     /locus_tag="WGR46_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856785.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(582..2153)
                     /locus_tag="WGR46_001910"
     CDS             complement(582..2153)
                     /locus_tag="WGR46_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0856786.1"
                     /translation="MLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGL
                     LTILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSI
                     DHIQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLR
                     NATEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLS
                     ALFEFSNKLLFSMKNIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYI
                     QIKMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFE
                     NINLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGL
                     SGIVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDM
                     GNILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAH
                     RQETIQSADKILNLG"
     gene            complement(2163..2667)
                     /locus_tag="WGR46_001911"
                     /pseudo
     CDS             complement(2163..2667)
                     /locus_tag="WGR46_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815283.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(2670..3935)
                     /locus_tag="WGR46_001912"
     CDS             complement(2670..3935)
                     /locus_tag="WGR46_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0856787.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQICLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3949..4119)
                     /locus_tag="WGR46_001913"
     CDS             complement(3949..4119)
                     /locus_tag="WGR46_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856788.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     ILIIGIFLSIFF"
     gene            complement(5158..5394)
                     /locus_tag="WGR46_001914"
     CDS             complement(5158..5394)
                     /locus_tag="WGR46_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0856789.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(6448..6732)
                     /locus_tag="WGR46_001915"
                     /pseudo
     CDS             complement(6448..6732)
                     /locus_tag="WGR46_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2147 a         1188 c         1162 g         2235 t
ORIGIN      
        1 cgatgaaaat aataggctgc ggtattttta ttaacgccta ctaactctgc tgccgttctt
       61 gcagttacac ctgcgacaaa tagctcaatg agtttatttt gtttataccg gcttagacga
      121 ctttttctca tagggataat tctaacttaa tttgaatttc cctagttatc taggacagcc
      181 ccttaaaaaa tatccctttg aaagattttg atgaagtttg tgaaaagtgg gagaaatcct
      241 ttaaagaaaa tggaaaagaa attgaatata atcaaaaata ttcaagaatt tatggtgaag
      301 ttcgtggagc tggctttaat ggcgatatcg gagaaaagct acatcgttgt gccgtgatgg
      361 aatgggctga aaatgagacc gtgaaattag cccaaaaatg ggaacaagag cagaaaaaac
      421 aacaaattca acagaaaaag gaaactgaaa aatcgccaaa acacaaagcc agtcgtgatg
      481 attgggaaat ggaacgttaa accgctattt tcatgtattc tattaaatta tttgaaaaaa
      541 gacttcactt tttgttaaag aaaaatgaag tcttgttctg ttcaacctaa attcagaatt
      601 ttatctgccg attgaattgt ttcttggcgg tgtgcaacca ttattttcat aataccaaga
      661 ctttttaggt tatggttaat tttttgttca ttttctacat ctaaatggct acttgcttcg
      721 tctaaaaata gaattttggg tcgtttatac aatgcacgag ccaagataac tctctgcttt
      781 tgtccacctg ataagatatt tcccatatcg ccaatcaagg tctcatagcc cattggcatt
      841 ttaagtatat cgtcatgtat ttgtgccatt tttgcacatt gttcaatgag ctccatattt
      901 gggctttcat caaaaaatga aatattttcc ccaatagaac ctgcaaaaag gacatcatct
      961 tggcgaacaa tcccgctcaa tcccctaata aaggatggag aaacttgata tatatcatgc
     1021 ccattaatac taactgtacc agtttcaggt tttaggctac ctgttaaaat gtttaacaaa
     1081 gtggacttcc cccgaccaga ttgtcctgtt aaaacaactg cttcattatc tttaaattcc
     1141 aaattaatgt tttcaaaaag atatggctca ttatcagcat atctgaatga gacgttttca
     1201 actttaagaa ccagttgttc attatctaat ttaggtatat gattatactt aataatttca
     1261 gtttctgttt catttaaagt aatgtcagcc aaacgttcag catgaagccc taacattttg
     1321 atttggatgt attggtcaac aagagaagct gttctgcttt caaattgccc tttataagcc
     1381 aaaaaagcca tcagaacacc gactgtaaat gaaccatcta aaattgcgct tgcaccaaga
     1441 taaattatga taatattttt catgctaaac aacagtttat ttgaaaattc aaataaagca
     1501 gagagtttat ctgttgtcag cttggtattg actgtattca caaataggct catccaagtg
     1561 ccatgtcttt gataatgttt atcaaataat ttaactgatt ggataccacg aatggtttcc
     1621 atgaaatatg agttttgttt ggcttcatga acaatatttt cttctgttgc atttcttaat
     1681 gggtaatatg caagccaacg aattagtatg tacaaaacaa gtgttaaaag aacaatcagc
     1741 gataattgag tgctgtaaat tgtcattaac acgaaagtaa aaacagccat taagctattt
     1801 aaaactaaaa caaaaaaagt agaagttagt gtttcttgga tatgatctat tgaaccaaat
     1861 cttgaaatca catctcctaa atgtcgttta ctgaaatagt cattaggtaa gtcaagtaac
     1921 cttttaaata tattggcttt ccattgtaaa ttaagagttg tagataggtg catacctacc
     1981 catgcttgta acaggctaat taactgttgc aggatagtca gtaaaccaaa tcccaaagta
     2041 agggtcaata ataaattttt atcagcagtt acaatgacat ggtctattac ccattgcata
     2101 aagaatggac taaccaatgc aaagacttcc aaagaaatag ctaatataag catttgaatt
     2161 aaagagcgtt ttaagcctgc ccccccttaa tagagataat attttgattt tctttgtttc
     2221 ttttttctct tcaaaatggg tattggggaa taattctagg gcaatccctg tgaatttttg
     2281 tgaaacttcg tccattttga tttttcgcat accgacagca gggtccataa tgacgatact
     2341 gtctttggaa atggaacaaa gtacaacaaa atggtttaag ttccaatgga gaatgcaggg
     2401 tagttgtaaa tttgacagct catctaactc taaacgcaaa gctcgtggcg ttaaattcat
     2461 ttcattgcca aatctcatga tgtctgcaag atttgcgccc tttaatgaca gggtgtattt
     2521 ttggcgtaac gttcttaaat cagtgtgaaa gccataatag gacaagatgg atgtcaggca
     2581 tgctaaacca cattcagcaa cttctgtttg cagaatgaca ggtagctttt tgttaaatcc
     2641 aaaggacagt cttgataaat aatccatttt taattgattt ttcctgaaat gctgtaaagt
     2701 gggtcaagta cccattcgta caattttttt cgttcatgga gaatatctgc ttctaaaatc
     2761 atgccaattt gaagcggctt gttttctccg tatgctttaa tcgtttgttt ttccaattta
     2821 actttcacaa gataggcagg ttcatttaat aaggttgggt tagtgaaaat gatacctaaa
     2881 cctgatagct cttgtttacc gagagcagtt ctggcaactg aaataatttc tcctgtggca
     2941 tgtccaaatt tttggtaagg gtacgcttgg taacgtaaaa caactttatc tttcggttta
     3001 ataaaaccaa cagctttact gggtatgtaa agattggcga ccaattcagt ttgttcaggg
     3061 acaatgctta acagcaattt agacggttca acttgttgcc ctatatcaac attaattgtt
     3121 gaaatataac ctgatttact agctcgtatg gtttgttcgg atttcaaatc aaaatccaaa
     3181 atttcttggt tcatttccgt aatcgcacgg ttgagttggc tcaattcggt tttatggcgt
     3241 tcaggcaggc tgcttaatgt gattttctgt tcatcaagtt ccctgattgc attattttgt
     3301 tcacgtttta ggctctccaa acgtgagcgt tgttccaata aatggctttc ggcggtcatc
     3361 ttatcttgtt gggatactgc gccttgactg gctaaaaact tgttcttgtt aagggttttt
     3421 tccgctaaac aaatttgacg attttgccct gtaatttgct gtttaatatt ctctaattga
     3481 ttgtttaaac gatgaatgtt gttatgaaca tttttttgct cattttgatg aatgcgcttt
     3541 aaacgttcca attcttgtaa tgccaaagtt tttttaaggt tggcttctgc tgccaatttg
     3601 gcttgtacgt ttcctttttc gccaaaacgc gatgtggaaa gtttgaacaa tggttcgcca
     3661 gctttgacaa agttaccatc ttcaacaaat ttatgcgtaa tcgtgccgat atcggaagag
     3721 taaacacgaa ccacccccat agttggaagt aattgacctt caacggttgt tttattggta
     3781 tagctaccaa aaatcaaaaa gataatgata cacagagcaa tgagaaaagc gcaaaaagtc
     3841 agaaataaaa aagagaatgg acgggtcaag ataacctgac ctgtccactt attttgttgg
     3901 gctacaaaga cttcttttcg gaataagtgg gacatttatt attatttctt aaaaaaaaat
     3961 ggatagaaaa ataccaataa ttaaaatact tatgcctaat aacataaatt tgctattgat
     4021 tgatgtttct attttaatta cattaaattt aaccaataat ccagaaatgg ttgcagctaa
     4081 aatgcaaaag acagtggcgt aaatggtttc agtattcata atttttctct ttcaaaattg
     4141 ttcaatctaa atagaaaaag gtaacttcaa tacatattgc tgtaactgaa attacctttt
     4201 ccttaatgat gtaattattt tatggcttca tcatgccacg accgatattg tatccgccag
     4261 cagcagagaa aataccagct cccattgcgt caacccagcc tactggtcct gtccacttgt
     4321 tccgttgagc tacaaagact tctggtctga aaaatgagtg attgttagac atgtgtactt
     4381 atttatgttt ctttgattgg gataaatttg taaaaacaaa aaaagcaagt tgttattata
     4441 gaagaactta aaatgcttaa catactcatt gaggtttgta ccacataatg agatacgaga
     4501 taatttgttg ggagtaaaat caaaaaaaag attaaatatt taaaataatc actatatttt
     4561 ttcattattt gttccttttt gtatttaagt tcttatgaaa aactagggta ttttgaatag
     4621 aaaatatcaa aataccctag ttttattaag gaatattacc accaacaagt gcttccatat
     4681 tgtacagcac cagtgattgc accaccaata gctccgaatt tcgcacctgg aatagcacca
     4741 ataccacctg taaatgaacc aacaattgcc ccaccagctg ctccacctaa tgcactacca
     4801 atggtatttt ttgagaaatc acgccagtta caagcagcac cagaaacttg ttaaatttca
     4861 ttcaaagtca aaacttgcat aatatttacc tttgttaagt gtggttgaga aaaaagtaaa
     4921 ttttcatcgt tactcattat ttgcaatcat gcatatactg taaccaagca ctatattcag
     4981 ttggatcata gtttttccta ctgggttgtc tgatatgact attggcacta ttggcacaat
     5041 tgggattagc aactttattg tgagtaccta atggattgat atttcctgta aagtggctat
     5101 tgcatgcgct taatgagcat agatttatta atgtcagaaa ggataaaaca aaaaatctta
     5161 ccatttttta ctaataccaa aagtaggttt cgaaaactgt acaccaagac cttgtacata
     5221 tccaaattgc ttgctaacag aaaactctac tccactattg ttaggggaat aggaagcacc
     5281 aattttacca acagcaacca tatctttttt acctaggttg ccgccaccct gtgcaaagat
     5341 atggaatttg ccacctgaca cttcaactaa ttcagaggtg tgtaactctt tcatgatttt
     5401 ctcctgtaaa gaagtatggt tagaaatgta aatcagaatt aacctttctt aagtttaatt
     5461 taatactttt tttgcaagta ttttatttta aattaaatca atattttaaa aatatttact
     5521 tgtatactga atatttactt gtatactgaa aaagaaactc ctaatggtgg agtggggctt
     5581 ttgtttaagt aagcattgaa aaaatttaaa tttcccatta tgattaactt aatgcaagag
     5641 ctttttcagg acgtacaaac cagcgaacgg gcgttctgat gtgggcaggt tggttgtcgc
     5701 taaccaattt cagtagctca atcgtcagat tggtatccga ccctatcaag ctaaggagag
     5761 gctgcggcta ctacacaaag cacctgaaat tattgggtgc ttttgtcttt ttatggcatt
     5821 gtaccaattt gtgtaccacg agggcttgtt cgcccaaacg cggtaacaac tgaatacaca
     5881 ctttccccct caacttcttt gcgttctaaa acgactgtcc caatccgaga atagacaatc
     5941 ataggacgat gattgcccct tacatcacta aattcactaa aaatttttgc gcctgattga
     6001 aaaatcattt ccacataaaa gggttacacc tgtttcatcg tggcttaatc caaaccgcgt
     6061 taaatctgct ttgtgttctg ccaaaatgtg aatcacacca aagccgtgat taaccttttt
     6121 gtgttcgccg tgttttaaaa caatttgacc tgccgagaag tctgctctat tgttcggaca
     6181 atagggggaa tataccaatt actttaacta catcattttt tgacattttt cttgcctttc
     6241 aatttaaaat ccaaaatcat actgccataa tttagcaatc caaaaaaatt taggcagcag
     6301 tcatggtttc gtattttaga aaacgaaact tggtttggtg ttttgaagca tttcagtaca
     6361 caaacatgga attttaaatt gtcttgtgtt gccttgattt aggatgttcc agttttttaa
     6421 taaatagttt ggggctgtcc tagataacta ggacaaactt gattttacta attgttttaa
     6481 aatggaacaa gaacttttat ctcactgttg ttaaaacgcc attcgcactc ctttaaatac
     6541 agctcaaaat gcgctttggg aatgccgtta aacttgcgta aatgacgttt tgcctggttc
     6601 caaaagttct caattccatt aatatggttt tgtcgttcag caaaatgtgt gctgtgattg
     6661 atacgaaaac gaagtttcag cgaagctaaa atggctaaat tcgcgcacat ctaatacatc
     6721 atagctacga ta
//
LOCUS       DAWFKN010000101         6711 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFKN010000101 DAWFKN010000000
VERSION     DAWFKN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.101"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(372..932)
                     /locus_tag="WGR46_001916"
     CDS             complement(372..932)
                     /locus_tag="WGR46_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0856790.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1349..2389)
                     /gene="mutY"
                     /locus_tag="WGR46_001917"
     CDS             complement(1349..2389)
                     /gene="mutY"
                     /locus_tag="WGR46_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0856791.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2552..3656
                     /locus_tag="WGR46_001918"
                     /pseudo
     CDS             2552..3656
                     /locus_tag="WGR46_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3679..3897)
                     /locus_tag="WGR46_001919"
     CDS             complement(3679..3897)
                     /locus_tag="WGR46_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856792.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3912..4550)
                     /locus_tag="WGR46_001920"
     CDS             complement(3912..4550)
                     /locus_tag="WGR46_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0856793.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4731..6566)
                     /gene="edd"
                     /locus_tag="WGR46_001921"
     CDS             complement(4731..6566)
                     /gene="edd"
                     /locus_tag="WGR46_001921"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0856794.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIHSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1508 a         1599 c         1889 g         1715 t
ORIGIN      
        1 gccaacaggc taagtgcgcc cgctgccgcc taaaaggcag cccggatgcc tgattatcgg
       61 gtatccgggc aggattaagg gggtatttgg gtaaaattag gaggtatttg gggcgaaaac
      121 agccgaaaac ctgtgttggg atttcggctg tcgggaggga aaggaatttt gcaaaggtct
      181 caaatagtac ggaatcgatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
      241 gctaaggcga gccaacgttg taccggttta aatttaattc actatactgc tgaagtcgtg
      301 aattaaatgc taaatatcta gcaaaaaagt gcggtcaaaa tttaccgcac ttttcgtttc
      361 gaacaatcga attatttaat cacgccacat gccatacgtg ggccgccacc gccaagtgga
      421 gctggatgat cggagtgatt atcaccaccc gtgtggatca taatagagtg accgcgaaca
      481 tcatctaaat gtttaagacg tggtgctaaa acaggatttg ttgctgtgcc atcatgcaat
      541 acagttaatg caggtaaatc acctaagtgt gcatcatctt gccatgggta accatgttgt
      601 tttgcacctt taggatccca gtgaccgcct gcgcctaaac cagctgtcaa tttaccttct
      661 ttttcttttg gctcacagct tgggttttca tggatgtgga aaccatgtaa gccttcgctt
      721 aatccttgta aatcaggggt aaacacaaga ccatagttag attcagtaat agtcactgta
      781 cccacatctt tgttaccgtt tactggatca agttgttgca ctttcacttc aatagaagca
      841 cctttaggta tcgtattatg ctcgtgtgct tgcgcaacac taactgaaca tactgcacta
      901 accgctagtg ctaataaggt tttcatattc attgcaatct cctttattgc agtaaaaact
      961 cgcgactatt ttagtcaatt ttgttttaaa agccgataaa tttttacgaa aattttacat
     1021 taattgttat ttgcagaaaa ttagaacccg ttatcacgtc ttggataaat gccaaaacca
     1081 ttgtgattca gagaattaaa aagaaaaatt tcggaatcct catccttttc tgaaacctga
     1141 ttgcggtatc catggctttg tctttgtcat ttaagtttgt gttcatgttt ttgaccttct
     1201 ttgcttttta aatccttttt aaattgccga cttcttccgt tccaaatgtc ccatttgaac
     1261 attatcccgc cgcctaaaat acgacccaaa ttcatcggtt gttcttgctg cgatacctgc
     1321 aaacaacagt tcaatgggtt tattttgttt attctaacct attaccgttt aaataaattt
     1381 ccaaaggctt gggcaggccg taatctttca aatgcgtcgg ctttatccaa atgccgtctg
     1441 aagggctttc agacggcatt tgtccttcaa acggcgtaat catcagcaac cggtgcgtca
     1501 gccggtgggt cagggcggtt tgttcgtcca tatctgccat ggtcagggag aatttggcgg
     1561 caaagtcgga aagcccgttc aaactttcaa aacacggcac gcaatacagc ccgccccaaa
     1621 tgcctttggc ggggcgtttt tccagcaaaa tcgcgccgtc ccggttgcgg acaatcagcc
     1681 agtaaagcgg cagggtcggt acttcggcgg cggttttttt gcgcggcagc tcggcggtgc
     1741 ggttttgctt tttcgcttcg cagatgtccg ccatcgggca ttggtggcac aagggtttcg
     1801 tccgtttgca cacggtcgcg cccaaatcca tcaaaccttg tgtataggca ggcatatcgg
     1861 cgttttcaga cggcagcagg ctttcggcaa gtgtccagag cgagttttca aattttttgt
     1921 cctgcggatt gccgtcgcgg gcgaacacgc ggcagagtac gcgtttgacg ttgccgtcca
     1981 aaatggtttc gcggcggttg aaggagaagg cgcaaatggc ggcggcggtg cttctgccta
     2041 cgccgcagag ggtttccaag tctttgcgct ccgacggaaa cgtgccgccg aattgttcga
     2101 cgacttgttg cgcggctttg tgcaggttgc gcgcgcggct gtaatagccc aagcccgccc
     2161 acaacgacaa cacttcgtct tgcggcgcgg cggcaagcgt ctgaacggtc gggaattttt
     2221 ctaagaaacg cggatagtag tccaacacgg tggcgacctg cgtttgctgg agcatgattt
     2281 cggaaagcca gacgcaataa gggtttttga cctgccaagg gaggtggtgg cgaccgtgtt
     2341 gtttttgcca gcggatgagc cgttcggaga aggggatggg tgtgttcatt aatatcaatc
     2401 ggtggtttta tttatattta aaacagtatg ttattgtata acattgtgaa aataattctt
     2461 attgacttat tttttgtagg ggcatataac tcatataagg aaactttatt gcggtattga
     2521 aattatttat caacaagcaa ggagtatcag catgaaagca atggtttatc acggcgcaaa
     2581 cgacatccgt tttgaagaaa aaccccgtcc gcagattatc gatccgaccg atgcggtggt
     2641 gaaaatcgtc aaaaccacga tttgcggtac cgatttgggt atttggaaag gcaaaaaaac
     2701 ccgaagtcgc cgacggccgt attctcggtc atgaaggcat cggtattgta gaagaagtcg
     2761 gcgaggctgt aaaaaacatc aaagtcggcg ataaagtcat tatttcatgc gtcagcaaat
     2821 gctgtacttg cgacaactgc aaaatccaac tttattcgca ttgccgcaac ggcggctgga
     2881 ttttgggcta catgattgac ggcacgcagg cagaatacgt ccgcacgcag gcagaatacg
     2941 tccgcacgca ggcagaatac gtccgcacgc aggcagaata cgtccgcacg ccttatgccg
     3001 acaacagcct cgtgccgctg cccgacaacg tcaacgaaga agtcgccctg ttgttgagcg
     3061 acgctttgcc gaccgcacac gaaatcggcg tgcaatacgg cgatgtcaaa cccggcgaca
     3121 ccgtattcat cgcaggtgcc gctcctgtcg gtatgtccgc cttgccgacc gcccaactgt
     3181 acagccccgc cgccatcatt gtttgcgata tggacgaaaa ccgtttgaaa ctggcgaaag
     3241 agttgggcgc aacccatacc attaaccctg cttccggcga tgtaagcaag caagttgccg
     3301 ctatcgtcgg cgaagacggc gtggactgcg cgatcgaagc cgtcggtatc cctgcaactt
     3361 ggaatatgtg ccaagacatt gtgaaacccg gcggtcatat cgccgttgtc ggcgtacacg
     3421 gtcaatccgt tgatttcaaa ctggaaaaac tgtggattaa aaagcttgcc atcactaccg
     3481 gcctggtaaa cgccaatacc accgaaatgc tgatgaaggc aatttccagc agctccgtcg
     3541 attacaccaa aatgttgacc caccgtttca aattcagcga attggaaaaa gcctacgacg
     3601 tgttcaaaca cgccgccgaa aatcaagcga tgaaagtggt tttggaagcg gattaatcgg
     3661 cgttgaatca gttgtttttt aagcggatgc cgtctgaatg cgtttcagac ggcatttttt
     3721 cagccgcgta gtatgggctg tccggctgct gtcgaatgaa ctgaacgtta tttttattat
     3781 gcggaaggcg gatgggcatt ttaggggcag tatgtcggag tggtgctgcc gtgtatgccg
     3841 actgcgccgc caagcggcaa aatgccgtct gagactggaa agggcttcag acggcatttt
     3901 tacgatgcga ttcaggcttt gggtttcaat gccgccgctt ctttggcgag acgggtgatg
     3961 gtgtcccagt ctttgttttt cacggcttct ttcggcgtca gccatgagcc gccgacgcac
     4021 aggacgttgg gcagtgccaa gtaatcgggc gcggtggcga ggctgatgcc gcctgtcggg
     4081 cagaagcgaa catcggcgta agggccgtac agggctttga gcatggcttt gccgccaacg
     4141 acttcggcag ggaagagttt gagggtgtcg atgccgtgtt ccaaagccag ttggatttcg
     4201 cccggggtgg caacaccggg aatcaggggg atgccgctgt tgtggccggc tttggcgagg
     4261 gattcgtgca aacccgggct gatggcgaaa accgcgcctg cgtcttcgac ggctttgagc
     4321 tgttcagggt tggtaaccgt acccgcgccg acgatggcgt tgggcacttc tttggcaatc
     4381 aggcggatgg catcgaggcc gacaggggtg cgcagggtga tttcgagggt ggggatgccg
     4441 ccttcgacaa gggcgtggga caaatcgacg gcggtgctta agtcgtcaat cgccattacc
     4501 ggcacaactg cgccggcggt caaaatttcg cggggggtca gtttggacat ttcggttctc
     4561 cgggtggaat ggggtatttt attaagatgg gacaggtcgt aggtcggatt caagaaaccg
     4621 acatcttgga cggctgtgtc ggattcgaga atccaaccta cgtctatctt aaaacacttg
     4681 aataattttt cagacggcct tcggtctgaa aaggccgtct gaaacgcgca tcaggcaaat
     4741 tcgccgccga aactcatggc accggtttcc gcgctgctgg tcatgctgcg gaagttggcg
     4801 aagagttcgc ggccgcagcc ttgttggttc gcgcccaagt cgatgctttc gacttcgcgg
     4861 gcattccatt cggtttcgtt aatcaggacg ttgagttcgc cgctaacgga gtcgaagcgg
     4921 atcaggtcgc cggtacggat tttggcgatg ttgccgccca tcagggcttc gggtgtcatg
     4981 tggatggagg ccggaacttt gccagatgcg ccggacatac gaccgtcggt cagcagtgcc
     5041 actttgaagc cgcggtcttg caggatgccc aaaggcgggg tcagtttgtg caattcgggc
     5101 ataccgttgg cacgcgggcc ttggtagcgg acgacgcaga cgaaatcgcg ttccaactcg
     5161 ccgcgttcaa atgccgccaa cacttcgcgt tggtcgttga acacgatggc aggcgcttca
     5221 ataatgcggc agccttcgcg cacggcggac actttaatca cgccgcgtcc gatgttgcct
     5281 ttcatcaggc gcagaccgcc gtcgggggag aacgggttgc cggctttgcg caggatgtcg
     5341 tcgttgccgc tggtttcggg ggcttcgcgc cattcgagtt tgccgtcgat aaggaaaggc
     5401 tctttggtgt agtggcgcat accgtgtccg acaacggtat cgacatcgtc gtgcaacagg
     5461 cctgcattca gcaattcgcg gataacgaaa ggtaggccgc cggcagcggt aaagtggttt
     5521 acgtcggctt tgccgttggg gtacacacgg atcagcaatg ggatgatgga agaaatttcg
     5581 tcaaagtcgt cccagttcaa aatcacgccg gcggcgcgtg ccatggcaac gaggtgcatg
     5641 gtgtggttgg ttgagccgcc ggttgccatc aggccgatta gggcgttgat gaaagatttt
     5701 tcggtcaaca tttcacccaa tggtttaatc gtgccgtttt tgatgccgcg cgcgaggtgt
     5761 ccggcggcat agcgggtcag ggcttcacgc aggtcggtgt aagggtggac gaaggcggcg
     5821 gcaggcaggt gtacgcccat catttccatc atcatttggt tggagtttgc cgtgccgtag
     5881 aaagtgcagg tgcccgggct gtggtaagaa cccatttcgc ttttcaaaag ttcgtctcgt
     5941 ccgactttgc cttcggcgaa aagctggcgg gtgcgggctt tttctttgtt gccgatgccg
     6001 ctggacatcg ggcctgcggg gacgaagata cccggaatat gaccgaacga aagcgcgcct
     6061 atcatcaaac ccggcacgat tttgtcgcat acgcccataa acaggctgcc gtcaaacatt
     6121 tgatgcgaca gaccgatagc ggtactcatg gcgatcacgt cgcgggaaaa cagcgacaat
     6181 tccataccgg cgtagccttg cgtgatgccg tcgcacatgg cgggtgtgcc gccggcgact
     6241 tgggcggtcg cgccgttttt ctgcgcttcg tctttaattt ggtcggggaa gtctttaaac
     6301 ggctggtgtg cggaaaccat gtcgttgtag gcggtgatga tgcctaagtt ggggacggtt
     6361 tcctggagca tttcgatttt gatgcttttg ggcatagagg catagccgtg cgccaagttg
     6421 gagcagccga gctggttgcg ctctaagcgt cccatttgtt tggcactgtg gattttcgcc
     6481 agatattttt cacgcgtcgg acggctgcgt tcaataatgc gcccggtgat ttcggcgagt
     6541 ttggagtgga taggagtagt gttcacggtc ggtctcctgt cggaacgggg ggtattgggg
     6601 atttgtttgt gcgtttctga ttttttgtat tgggtgtggg gcatttggcg tttcagacga
     6661 cctttcaaac cttacaaggg gtcgtctgaa aacctcacta tagtggatta a
//
LOCUS       DAWFKN010000102         6553 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFKN010000102 DAWFKN010000000
VERSION     DAWFKN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.102"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(146..1501)
                     /locus_tag="WGR46_001922"
     CDS             complement(146..1501)
                     /locus_tag="WGR46_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0856795.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1698..2519
                     /gene="nadE"
                     /locus_tag="WGR46_001923"
     CDS             1698..2519
                     /gene="nadE"
                     /locus_tag="WGR46_001923"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0856796.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2589..3062)
                     /locus_tag="WGR46_001924"
     CDS             complement(2589..3062)
                     /locus_tag="WGR46_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0856797.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3365..3697
                     /gene="trxA"
                     /locus_tag="WGR46_001925"
     CDS             3365..3697
                     /gene="trxA"
                     /locus_tag="WGR46_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0856798.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWASWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3750..4697)
                     /locus_tag="WGR46_001926"
     CDS             complement(3750..4697)
                     /locus_tag="WGR46_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0856799.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            5025..6413
                     /locus_tag="WGR46_001927"
     CDS             5025..6413
                     /locus_tag="WGR46_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0856800.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1566 a         1765 c         1725 g         1497 t
ORIGIN      
        1 aacgtatcaa tcgtcattcc cgcgcaggcg ggaatacggt tttttgaatt tcagtcattt
       61 ctgataaacc ccggcggctt tccgcttccg gactcccgct tttgcgggaa tgacggcgga
      121 gggggagcgg tttttccgat tgggtttaaa tgcaatcgaa caaatcctgc tgcccttgtt
      181 ctttgcttac gcgcacgtcg gtttcgccgt cggcgaaggt aatgtgcagc ttctgcccct
      241 gcttcaaaac atcggcgttg cggatgactt gtccgcgtgt gtttttgacg acggaaaagc
      301 cgcgctccag aatgtgctgc ggcgaaacgg cttcgagcaa tgcggcttgg gcggtcaggc
      361 tttggcggcg gtgggtaagc agttggctga aggcggcggg caatgccgtc tgaaggcgga
      421 cgatgttgtt tttgcaaacg gaaacatcag gacggtaatg ttgcagggct tgggtttggc
      481 gttcaaaacg ggcggtgtgg gcacggaggt tttgcttcat cgagtaagac agcgtttgcg
      541 ccagcttgcc gattgaagcg cgctgctcat caagtttttg gcgcggatga cggatttgcc
      601 gcgcaagcca gtcgagtttt tggctggcat cgaaatagcg ttgttccaaa acggttttca
      661 gacggccttg cgcctgagcg agacgatgta gcgattcttg gcggttgggg ctgaccagct
      721 ccgccgcacc ggtcggcgtg ggcgcgcgca cgtcggcgac aaaatcggca agcgtaaaat
      781 cggtttcgtg tcccacgcca ctgacgacgg gaaccgtgca ggcttcgatt gcgcgcacga
      841 ccggttcttc gttaaacgcc cacaagtctt caatgctgcc gccgccgcga cagacaatca
      901 acacgtcgca ttcggcgcgt tgcgaggcgg ttttaatcgc ttgggcaatt tgtaactcgc
      961 tgcctgcgcc ttgaacaggt gtcggataaa cgataacggg aatttcgggc gcgcggcgtt
     1021 tcaaggtggt aacgacatcg cgcaatgccg ctgctgccag gctggtgacg atgccgatac
     1081 attgcggacg ggtaggcaac ggctttttgc gttccgccgc aaacgcgcct tccgcctgca
     1141 actgtgcctt caaccgctcg taggcttcgt aaagctgccc caaacctttg agccgcacct
     1201 cattcacggt aatttgaaat tcgccccgcg cctcgtaaat actgattttt cctgatactt
     1261 cgatatggtc gccttctttc aaaggcttcg ccaaacgcgc cgccgcacct ttgaacatcg
     1321 cgcaacgcac ctgcgcgcgg ctgtctttga gcgagaaata ataatgtccg ctggcggcac
     1381 gggtcaggtt ggacacttcg cccgcaatcc acaggccggc aaggtggttt tccaaaagac
     1441 ttttggcaaa tgcgttcaac tcagaaacgg acaacacgtc agaatgaaaa aaatcagaca
     1501 tagaatcaat caaataataa aaaatatgaa tatgttttga agcctaaggc ggtattagtc
     1561 cgcctaaatt gtcaacaata ttataacacg cgccatcttg ccgcccgcct tttcccgtat
     1621 gactttttta agcggggaat gggaaaaata ttcatcaacc cgccctcaat ctattcaaat
     1681 tacaccgccg acaagctatg atgcggatat tttcgacagg aggaaaaatg gatacgcagg
     1741 cagttatcac acatattgtc cgatggttgg acgaatacgc cgcccgggca aatgcaaaag
     1801 ggtttgtcgt gggcgtttcc ggcggcatcg attccgccgt cgtctccgca ctcgccgccc
     1861 gcaccggccg ccccacgctg cttctggata tgccgatacg ccaacacccc gaccagcttg
     1921 agcgggcaag gctgcacatc cgcaatctgc aacggcaata tgccaatgta agcgcacaaa
     1981 cggtcgatct gaccgacacc ttccagacct ttgaacaaac cgtcggcgtt catcagacgg
     2041 catttgacaa tcagccgctt tccctcgcca acgccagaag ccgcctgcgt atgctgaccc
     2101 tgtactacta cgggcagata cacggactac tggttacggg gacaggtaac aaaattgaag
     2161 atttcggtgt aggatttttt accaaatacg gcgacggcgg cgtggacatc agcccgattg
     2221 ccgacctgac caaaacccag gtttaccggc ttgccgaagc attgggcgtg gacgaggcga
     2281 ttcaaaaagc cccgccgacc gacggcctgt gggatacgga acgcaccgac gaagaacaga
     2341 tgggcgcaag ctatcccgaa ctggagtggg caatgggcgt gtacggcacg cgcaaacccg
     2401 aagattttga agggcggcag cgcgaagttc tggaaatcta tacgcggctg caccgcgcga
     2461 tgcagcacaa aatcaacccg attcccgtgt gccgcattcc gcccgaattg ttgggctgaa
     2521 acgtggaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcattgac atatccggct
     2581 cctatccctt aagaatcgag tacgcgggca aacaaaatat cgttttccaa atgaatgtgg
     2641 tcgttcaaat cctccaccat ttctttggcc aaagcgtaaa gccgcgtcca gcttccgcag
     2701 gctccttcgg gcgcttggaa attatcggtc agctctttga gccttgcgat ggcgcggtcg
     2761 tgttcttcat gttcgtgcat catcacgctg atgggcatcg ccgcaccccg accgacaccc
     2821 tgattaatca tcggaaacag catcctttcc tctttcatca tatgcatcag cagttcgttc
     2881 tgcatatagg caagcagctc ggcaatttcc gccggaaagg tatcggcatg aacttgggcc
     2941 actttttgcg ccagcggcac caattcttca aattgtgcac ggtgtacatt gtggtagcgt
     3001 tgcaggatat gatcgacggt tgcaccaaag ggagcagttt cccaaacgga aaaatcagtc
     3061 atggcagggt tccttttatt tatagggttt cgggtttggt tttgaacatt catactttaa
     3121 gaatctattt aaacggagca tacaccgccc gtgcgtttct gtacagcttc aaaaacattc
     3181 cttacatttt gataataaaa gtaattttca aaaataaaat actgtccgaa ccgttttttt
     3241 agaatttgca aaggcgattg gggcggtaca gaaaaactat tatcccgccc gcccacttga
     3301 aatttttatg cccaagccct atcctgcacg ctatcgtgcc aatcccaacc gaaaaggaaa
     3361 aataatgagc agcgaattga tcgtacacac cggcgatgcc gccttcgagc aagatgtttt
     3421 gaaatccgac ctccccgtcc tgctggactt ttgggcttcg tggtgcggcc cgtgcaaaat
     3481 gattgccccg attttggacg acattgccgc cgaatttgaa ggccgtctga aagtagtcaa
     3541 aatcaacatc gacgacaacg aagccacccc gtcccgtttc ggcgtgcgcg gcattccgac
     3601 cctgatggtg ttcaaaaacg gcgacgtcgt cgccaccaaa gtcggcgcat tggcaaaagg
     3661 tcagctgacc gcctttgtcg aagcctctat cgcctgataa agcgcaatcg aaaaagccgc
     3721 cggaagattc cggcggcttt ttcgcaccct taagatttgt ggcggatttc ccagcaccga
     3781 tggatttttt tgttgcggaa atcttcggga acggattgtt tggaaatgtc tttgacggcg
     3841 tattgttccg ataccaagtc gtctaagacg aagctgcgca ggttgttgga aaagtacaaa
     3901 atgccgtctg aagcgagcag cttcaccgcg ccgtcaatca gctttttgtg atcgcgctga
     3961 atgtcgagga tgtcggacat tttcttgctg ttggaaaaac tgggcggatc catcacaatg
     4021 aggtcgaact gcctgccttc cccatatgcc gtctgaagat attggaacac gtcggcgcgg
     4081 acgattttgt gccgttccgt atcgatgccg ttcaattcaa aattgcgtct cgcccaatcg
     4141 agataagtgt tggacaaatc gacagtttcg ctggatgccg cgccgccggt ggcggcatag
     4201 acagtgaagc tgccggtgta ggaaaacagg tttaaaaaac gtttgcccgc cgccgtttcg
     4261 ccgacttttt tgcgcgtgtt tcgatgatcc aaaaaaagcc ccgtatccaa atacttatca
     4321 aggttgaccc aaaacttgcg gccgttttcg gtgatgacga aatcgtcgcc cgccttgccg
     4381 gttttttcgt attgctgcaa acctttttgg cgttcgcggc gtttgaggcg gatttgttcg
     4441 ggcgcaaaac cggtaacgaa ggcgacggct tccaagactt cggcaagcca cgcttcgtat
     4501 tcttcggacc gcatcagcca gccggtatcg tattcctgaa ggtggatttg gtcgccgtaa
     4561 acatcgacag caaaggggaa ttgggggatg tcgcggtcgt aaatgcgcca ggcttcgatg
     4621 ccgttgcgtt tcgcccattt cataaggtgt ttgatgtttt tgcccaagcg gttggcaaac
     4681 ggtgtgatgt cggtcattgg tttcaggcgg aataaagtgg aaaacggcaa ttttactgta
     4741 attaacgccc gattgcttga ccgtttcggg caaaccctat accatccgtc gcttatcttg
     4801 tcatacgaag ccatcgcctt ccaacctaaa ccgcccttac gggcgcgttt cttctgttgc
     4861 tttgattttg caaagcgtat ctgtgcaggt tgccgtcgat gtaaaccaca agcaagccgc
     4921 ttgcgacaac cctgtaactt cacattcccc gtatcgttac ccttccctgc ttcaggccgt
     4981 ctgaaccttt cggacgcgag cgttgttgtc ttccaaggat agccatgtct attaaatttg
     5041 ccgatttgaa ccttgataaa aacattttgt ccgccgtcag cagcgagggt tacgaaagcc
     5101 cgacgccgat tcaggcgcaa gccattccgt ttgctttgga aggccgcgac atcatggctt
     5161 cggcgcaaac cggctccggc aaaaccgccg cctttctgtt accgactttg cagcgactga
     5221 ccaaacgcag cgaaaaaccg ggcaaaggcc cgcgcgcgtt ggtgttgacg ccgacccgcg
     5281 aattggcggc tcaagtcgag aaaaacgcgc tggcgtatgc caaaaatatg cgttggttcc
     5341 gtaccgtcag catcgtcggt ggtgcatcgt tcggctacca aacccgcgcc ctgagcaaac
     5401 cggtcgatct gattgtcgcc acgccgggcc gtctgatgga cttgatgcaa agcggcaaag
     5461 tcgatttcgc ccgcttggaa gtgctgattt tggacgaagc cgaccgtatg ttggatatgg
     5521 gctttatcga cgacatcgaa accatcgtcg aagccacgcc ggaagaccgt cagactttgt
     5581 tgttctccgc cacttgggac ggcgcggtcg gcaaactggc gcgcaaactg accaaagacc
     5641 ctgaaatcat cgaagtcgaa cgcgtggacg accaaggcaa aatcgaagaa caactgctgt
     5701 actgcgacga tatgcgccac aaaaaccgcc tgctcgatca tatcttgcgc gatgccgaca
     5761 tcgatcaatg cgtgattttc acgtccacca aagccatgac cgaagtcatc gcggatgaat
     5821 tgtacgaaaa aggtttcgcc gccaactgcc tgcacggcga tatgccgcaa ggctggcgca
     5881 accgcacgct gatggatttg cgcaaaggcc gctgcaaaat tttggttgcc accgacgttg
     5941 ccgcacgcgg tatcgacgta ccgaccatta cccacgttat caactacgac ctgccgaaac
     6001 aggcggaaga ctacgtccac cgcatcgggc gcaccggccg cgcaggccgc acgggtattg
     6061 cgattacgtt tgccgaagtg aacgaatacg tcaaagtcca caaaatcgaa aaatacatta
     6121 accgaaaact gcccgaactg accatcgaag gcatggaacc gacccgcaaa cgcaaatccg
     6181 caggcggcaa gccgaaaggc aaaggcggct ggggcgatcg taaatccggc ggttggcgcg
     6241 gcgatcataa accgagcaaa gaaggcttcg gcggcaaaac gcgcggcgaa ggtttcaaga
     6301 aagaaggctt taagagagac ggtttcaaaa aaaccggcga aggcttcaaa ggcaaacgca
     6361 aagccggcga ttcttttgca ggcaaaggcg aacgccgtta caaagaccgc taagccccaa
     6421 cctgccgcat aaaccaatgc cgtctgaaac cgatttcgag tttcagacgg catttttaca
     6481 atgtttcagc accgcccggc tttgataccc aaaggattaa tctgcaatat agtggattaa
     6541 caaaaaccag tac
//
LOCUS       DAWFKN010000103         6424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFKN010000103 DAWFKN010000000
VERSION     DAWFKN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.103"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..687
                     /gene="mafB"
                     /locus_tag="WGR46_001928"
     CDS             <1..687
                     /gene="mafB"
                     /locus_tag="WGR46_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0856801.1"
                     /translation="PAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALV
                     NVLPFAKVKNLTKAAKLGKAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIH
                     EKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEIS
                     DIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDR
                     TSNPKYNPVK"
     gene            702..932
                     /locus_tag="WGR46_001929"
     CDS             702..932
                     /locus_tag="WGR46_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0856802.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1081..1755
                     /locus_tag="WGR46_001930"
     CDS             1081..1755
                     /locus_tag="WGR46_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0856803.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNECPTKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDADTGNHVYVKGEKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            1733..2014
                     /locus_tag="WGR46_001931"
     CDS             1733..2014
                     /locus_tag="WGR46_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856804.1"
                     /translation="MENGHRNNRSPLEKRIFYLEHSGQYLMICALSDYSKNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            2070..2243
                     /locus_tag="WGR46_001932"
                     /pseudo
     CDS             2070..2243
                     /locus_tag="WGR46_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            2224..2463
                     /locus_tag="WGR46_001933"
     CDS             2224..2463
                     /locus_tag="WGR46_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856805.1"
                     /translation="MVDAVVKTPEFVPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLIAVKKKQNYFDILLSGAIFYGR"
     gene            2453..2680
                     /locus_tag="WGR46_001934"
     CDS             2453..2680
                     /locus_tag="WGR46_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856806.1"
                     /translation="MGVNLIGLNFEDFKQLFHHPKSYGVDEYYLSNESYPTYVYEFDE
                     IGVQAWEAKGKVVTIIAGGKDNYSTEPYYDE"
     gene            2687..2743
                     /locus_tag="WGR46_001935"
                     /pseudo
     CDS             2687..2743
                     /locus_tag="WGR46_001935"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2749..3126
                     /locus_tag="WGR46_001936"
     CDS             2749..3126
                     /locus_tag="WGR46_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0856807.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            3431..3832
                     /locus_tag="WGR46_001937"
     CDS             3431..3832
                     /locus_tag="WGR46_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856808.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            3998..4447
                     /locus_tag="WGR46_001938"
     CDS             3998..4447
                     /locus_tag="WGR46_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856809.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPKNNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
     gene            4621..5754
                     /locus_tag="WGR46_001939"
     CDS             4621..5754
                     /locus_tag="WGR46_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856810.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(5841..6117)
                     /locus_tag="WGR46_001940"
                     /pseudo
     CDS             complement(5841..6117)
                     /locus_tag="WGR46_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6134..>6424)
                     /locus_tag="WGR46_001941"
     CDS             complement(6134..>6424)
                     /locus_tag="WGR46_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0856811.1"
                     /translation="EAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEEDN
                     YSTSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
BASE COUNT         2135 a         1174 c         1375 g         1740 t
ORIGIN      
        1 cccgccgagg gcaaattcgc cgccatcggc ggcttgggca gcgtggcggg ctttgaaaag
       61 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc
      121 gaagccctgg tcaacgtcct gccgtttgcc aaagtcaaaa acctgacaaa ggcggcaaaa
      181 ctggggaagg ctgcggttag cggggatttt tctgctgcat acaatacaag aacaactaga
      241 aaagttacta cagaaacaga ggggttaaat agaatcagac agaaccagaa aaatagtaat
      301 atacatgaga aaaattatgg aagagataat cctaatcata ttaatgtttt atctggaaat
      361 tctatacaac atatactgta tggagatgaa gcaggaggtg ggcatctttt tcctggcaaa
      421 cctggtaaga caacattccc ccaacattgg tcagccagta aaataactca tgaaattagt
      481 gatatcgtta catccccaaa aacgcaatgg tatgcacaga ctggaacagg cggcaaatat
      541 attgctaaag gaagaccagc taggtgggta tcatatgaaa cgagagatgg aattcgtatc
      601 agaacagttt atgaacctgc aacaggaaaa gtggtaactg cattccccga tagaacctct
      661 aatcccaaat ataaccctgt aaaataatga gtatttgaaa gatgaaaaca ttagacgaac
      721 gaataaaaaa tttaggaaaa tctcttgaag atagaattga cgctaatcta attgatgccg
      781 cactagagta cattactttt tcagaacgtt tgctagcttt tgaaacgcta tgtgattata
      841 tagaagattt caatatccag cttacggaaa aagaatccca agaaatctct tttattaata
      901 aggaatttgg aattgaaagt acatcagatt aataaatatt aggtcggttt aaaaacccaa
      961 caatcaaaaa gtcgtctgaa actgtgttta atttacagac gacctttcct tcatttgaag
     1021 caggatattg agaactgagt tcttcaaaaa tcctacacct gctccttcca cggcagcacc
     1081 ttggtcaaaa cggcagacgg ctacaaagcc attgcccata ttcaagccgg cgaccgcgtc
     1141 ttcgccaaag atgaaaccag tggaaaaact ggatacaaac ctgttaccgc ccaatacggc
     1201 aatccgtatc gggaaaccgt ttacattgaa atttcagacg gcatcggcaa caaccaaacc
     1261 ctgatttcca ataaaatcca cccgttttac agtcaaggaa aatggataca ggcaggtcgt
     1321 ctgaaaaaag gcgacaccct gctttccgaa agcggtgcaa aacagacggt tcaaaacatt
     1381 accctcaaac agcagccgct caaagcctac aacctgaccg ttgccgactg gcatacctat
     1441 ttcgtcaagg gtaatcaggc ggaaacggaa ggggtttggg ttcataatga gtgtccgacc
     1501 aaaccaaaac caactaatca tgcccaacaa agaaaagaag aagctaaaaa cgattctcat
     1561 cgaagtgtgg gagattccaa tcgtgtcgtt cgcgaaggaa agcaatattt agatgccgac
     1621 acaggaaacc atgtttatgt aaaaggagaa aaagtggtta ttctaactcc tgatggaaga
     1681 caggtaactc aatttaagaa ctcgaaagcc aatacgtcaa aaagggtaaa aaatggaaaa
     1741 tggacaccga aataatcgat ctcccttaga aaaaagaatt ttttatctgg agcattcggg
     1801 acagtatctg atgatttgcg ccctatcaga ttactccaaa aataaacata ctgtcgttat
     1861 ggcaaatttt ctctatccaa atgaaaaaat ggattggaga aatttagatg atttgttcaa
     1921 tgagttggtt ttagaggaat tacagtcttc atttatggat tggtatccaa ctattgaaga
     1981 ggcaattagc catcatttag aagatttttc gtaacaagca taaatcaaaa agtcgtctga
     2041 aactgctttt aattttcaga cgaccttgag atggcggtcg aagtcaaata tcagcggaat
     2101 caaaagttgc tgaattagaa aataaaatta ttcccggaat gcccaaaaat gaacgcttaa
     2161 agattaagca aaaaatcaga aatattacaa aaaatgccaa tcgaaaagca aaaggagaag
     2221 agcatggtcg acgcggtcgt taaaactccc gaatttgtac cttttacttc tgtaggaata
     2281 ttccgttttg gtgcggatat aacacaatac aaaaacatac tggaaacttt tatgtatgaa
     2341 cctcctgatg agtttagaac agaatattat gaatctcccg actcaaattt actcattgcc
     2401 gtcaaaaaaa aacaaaatta tttcgatatt ttgttatcag gagctatatt ttatgggcgt
     2461 taatttaata ggtctaaatt ttgaagattt taaacaactt ttccatcacc ctaaatcata
     2521 cggtgttgat gaatattatt tatcaaatga atcttatccg acatatgtct atgagtttga
     2581 tgaaataggg gtacaagcat gggaagcaaa agggaaagtt gttactatca ttgccggggg
     2641 taaagacaat tattcaacag aaccttacta cgatgaataa cattacagaa aagaaacaag
     2701 aaacggtaca tttgataaaa acctaaatcg tattggagat taattattat gtgtgagttc
     2761 aaggatttta gaagaaacat cccttgtttt gaagagtatg acgaaaattc atttattggc
     2821 aaatggtatg atgacggggt gtgggatgat gaagaatatt ggaaattgga gaatgattta
     2881 atcgaggtta gaaaaaaata tccttatccg atggatatac caagggatat tgtgattgga
     2941 atcggtacca ttattgattt tttaatggtt ccaaattggg agctttttga aattaaagct
     3001 tccccttggt tgcctgatag tgtgggaatt catgaacgtt atgaaagatt cacaacgatg
     3061 ctccgttata tttttaccga gaaagacata gtcaacgtgc gatttgatta ttacaacaaa
     3121 aaatagcaaa acccggcaat caaaatgccg tctgaagcga tatttgcttt tcagacggca
     3181 ttttgatgaa tgaaacagga tattaagaac taagttcttc aaaaatcctg cctccgtccc
     3241 ttatacgtcg gtgctttagt caaaacggca gacggctgaa aagcaaacac tgtcatcaac
     3301 catttgaaga ccggatggaa taggaaagaa aatcggtaaa atctccacga ttagacggta
     3361 aaatacctcg gtgtcgctga aaccctgatc gtcgtgttgc caaaacaaaa atcagatatg
     3421 gaaaacgata gtgaagatta aaccattaca attttctaac aatagtcgca gattttacgt
     3481 agacaaaatt tgtgttaccg aacaagatgt gaacattttg gcatccgacc gaaattatga
     3541 attaaatatt gaaatattta ttgacaatat aattcatttt caaataacag atgaatctta
     3601 taaagtaaaa ttttcagaat atttatttga aaataaagaa aaaaatgatt gggatagaaa
     3661 tcctgctata aattattttt tcgagataat agatgacagt tatatggact ggttgaaaga
     3721 agaaagtttt ggtttttttg aaaagaaata ttataaggct tacattttct tttttagcga
     3781 ttctgtaata gaagttatca gctcgacaga acctgtattt tattcaaaat aacaaattat
     3841 caaacaaagc tctgattaaa aacccaacaa tcaaaatgcc gtctgaagcg gtattcggct
     3901 ttcagacggc attttctccg attagcacag gatgccgggg cttgccggct tgattttgcc
     3961 gaaattgttg gcacgcaatt gggaggggcg gcaacctatg gaaaatagat ttgaaaaaat
     4021 cgttaaggat atttttagtt ttacccaatt tgtttccgaa gcagaaaatg agattcctaa
     4081 ccaatcctcc cgttattacg actgttggtg ggaattggaa atcttgaatg cttccgcatt
     4141 ggaagattgg gaaatatcgg gaaagcctga aaaatgggag aaatggacag aaaaatatca
     4201 gaccgaagcg ttacagtcag tcaagcatct atttgccgaa gaagttccta aaaataatat
     4261 agtttgcaat atttctcatt ttttgcattt tgccgatcgg gcagaagccg acattcccaa
     4321 tcaatcacgt tcatatgccg aatattggtg ggaaatggaa atgttcaaaa tgtcggtttt
     4381 agaagattgg gaacgctcgg gaagaccgtt aaattgggaa aaatggcaaa ggcaatatcg
     4441 atcttaagca tttgaattag ttgaaaattt gaatcaaagc aaacaagagt aacaagccgc
     4501 agcctgcata ggttttttta aagtacggca gcaagcgtag attagattaa aaattaaaca
     4561 atcaaagctc gtctgatata atcgatatat cctgttaaac gaattaaacg aaaaataaat
     4621 atgtttaaaa aattcaaact ggtattgttg tcattttttg cccttgtatt tgccttttgg
     4681 ctggggacgg gtattgccta tgagattaat ccgcgttggt ttttgagcga tacggcaact
     4741 gaaatacctg aaaatccgaa tgcttttgtg gcgaaacttg cccgcctgtt ccgaaatgcc
     4801 gacagggcgg ttgtcatcgt gaaggaatcg ataaggacgg aggaaaatct tgccgaaact
     4861 gtggatgacg gtccgttgca gtcggagaag gattatctcg cgctcgctat ccggctcagt
     4921 cgtttgaaag aaaaggcgaa atggtttcac gtaacggagc aggaacatgg gaaagaggtt
     4981 tggctggatt accatatcgg cgagggcggt ttggttgcgg tttcgctttc gcaacgctcg
     5041 ccggaagcgt ttgttaatgc cgaatatctg tatcggaacg atcgtccgtt ttctgtaaat
     5101 gtgtacggcg gaacggctca cggagaaaat tatgaaacga caggagaata tcgggttgtt
     5161 tggcaaccgg acggttcggt atttgatgcg tcggggcgcg ggaaaatcgg ggaagatgtt
     5221 tatgagcatt gcctcgggtg ttatcagatg gcccaggtat atttggcgaa ataccgggat
     5281 gtcgcgaatg atgagcagaa ggtttgggac ttccgcgaag agagcaaccg gattgcatcg
     5341 gactcgcgcg attctgtgtt ttatcagaat atgcgggaat tgatgccccg ggggatgaag
     5401 gcgaacagtc ttgtggtcgg ctatgatgcg gacggtctgc cgcaaaaagt ctattggagt
     5461 ttcgacaatg gaaaaaaacg ccagagtttc gaatattatt tgaaaaacgg aaatcttttt
     5521 attgcacaat cttcgacggt agcattgaaa gcggatggcg taacggcgga tatacagacc
     5581 tatcatgcgc aacagacgtg gtatttggat ggcgggcgga ttatccgcga agagaaacag
     5641 ggagacaaac tgcctgattt tcctttgaac ttggaaaatt tggaaaaaga ggtgagccgt
     5701 tatgcagagg ctgcggcgag acgttcgggc ggcaggcgcg acctttctca ctgaacgcaa
     5761 atgccgtctg aaaccggttt tcagacggca ttggagagtt tgacgctttt tgcaatgccg
     5821 tatattccaa atccaattcg ctacaaattg aaaaccacaa tccaaaaaac ggaaatacac
     5881 attaaacggg tctttttacc gaccgtatca tcccataggt tttccacccc tttttttcat
     5941 ccataaattc aaaccattta agtttgcatc cttaccgaca attgcagcag catcatccgc
     6001 ataacggtca cccaaattag ccaaataacg ccaaccgcca taggtatcgc cacggaaagc
     6061 ataaccacgc aaggtttgga tttgttctgg tgttaaaaca acttcaccct cattcataat
     6121 aagatccttt aaattatttt ttacttggat tgtttacaaa actctgtacc aattgctcta
     6181 cttgttggtg ataacctacc aactcctgct gtgtgagctg ctgcttttcc cgaataaacg
     6241 ggtaataccg taccgtgaaa ccgttaatac aggaacctac gctagtgcta taattatctt
     6301 cttcagtttg gtttattcga ttattccgat gagtaataat cgccggattt tctccttttt
     6361 taatatagta aagtttatga aaatatggat tttcaggaat atgtctttta tcttcataag
     6421 cttc
//
LOCUS       DAWFKN010000104         6234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFKN010000104 DAWFKN010000000
VERSION     DAWFKN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.104"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..69)
                     /locus_tag="WGR46_001942"
     CDS             complement(<1..69)
                     /locus_tag="WGR46_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0856812.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(211..2217)
                     /locus_tag="WGR46_001943"
     CDS             complement(211..2217)
                     /locus_tag="WGR46_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0856813.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2506..4533
                     /locus_tag="WGR46_001944"
     CDS             2506..4533
                     /locus_tag="WGR46_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0856814.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4625..6178)
                     /locus_tag="WGR46_001945"
     CDS             complement(4625..6178)
                     /locus_tag="WGR46_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0856815.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1282 a         1456 c         1887 g         1609 t
ORIGIN      
        1 tggagcagtt ctttctgtac ttttttcttt aatttgcagt gcgttatctt catatttcga
       61 gggtaacata tctgctaatc tagtacagcc ccttttaaaa tgccgtctga accacgtttc
      121 ggttcggacg gcatttttgc atccttttat ggggatttcc cgcatcaggg cggtattcgg
      181 ggcgaatccc gcttcagacg gcattgccgg tcaaggcttg tccgaaggag gatgtccctg
      241 ttctttggcg ggcgggagga aaagtatgca gggattgctt ttaatctgat tccaaaaact
      301 cttaatcaga ttgcggatgc tgccgatttt cgtgccgcgc gagcgcaggg cgacgaagag
      361 ggcgaggctg aagctgacgc agaggttgac caggccgatg gcgaggacgc tgaaaatgcc
      421 gagtacgaac gtgcccaaac cgacgttgcc gctgacggcg gcatagccga ggttggcgga
      481 ggaaaaggcg acgtggcgga tgtccagcgg cagcccgagg aggtgtccga aatagccggt
      541 catacccaag agcatcccga agatgaagtt gcctgccaac gagccgtagt gtttgtggat
      601 gtaggcggcg aggacgcggc ggggcccggg gcgcatgatt ttacgcagca aggggttgaa
      661 gggcaggcgt tggcgcaggt tgaggtagtc ggcgcggttg tcgaaaaaac ctgcgatgat
      721 gccggagcag aacagccaca gacctgcaat ggcggcatac cacagcgtcg ggtaagcgat
      781 gatgtctatg gatttgaact ggtaggcggc ggtgtgggca tcgagtatgg gctgccggta
      841 cagatgggca tagccgaacg atatggcgca cgccaaaagg atggcgatgg aaacgttgcc
      901 gaaaacggcg acactttggg aacggcatac gtcgatgagg agcttggcga gtttgttgtc
      961 caccgctttg ccgccttcgt tgagatcgac ctgttcggca aagctggcgg cagtcatcgc
     1021 gggctgtttg gtggcgacgg tgcagtgcag catatggatg atcataaagc cgatgccgta
     1081 gttgaaccca gccgacaagg aagtcaggaa ggggctgagg ccgagtgtgc cgatgcggat
     1141 tttgagcagc gccatcaggg cgatgatgat gccgccgcct gccgccgagt agaacatttt
     1201 gaaatattct ttgcggttgc gggtaatgta gtgttcgccg tggtggcttt tgttttcggt
     1261 aatgctgcgg gcgagcatac ggatgctgct gcggcgcagg tggcgggtgc tgtattgttc
     1321 gaccgccgcg tagatgaggg agttcatcag cgcgatggtc aggcggttgc tgttgccggc
     1381 gccggtttgg atgtcggtga gcagcttcag acggtctacg gtctgccgga gccgttcgag
     1441 caggtgggcg actttgaccg acgaaccgga gccggcgccc gtccctttgc ggcgcaaata
     1501 gtcaatctgg ctgaaacatt ggtcgaacat cacttcgagg tgggcggtgt cgtaaggcgt
     1561 ggtgccgttg cggtagtgtt cgaccagttt ggcggtttcg cgttggaggg cgacgaagga
     1621 agaatcggct tccagcaggc gcggggcgat gcggatgagg tcgggttcga tggcttcgga
     1681 tgccgtccag acagacagca tttcgatggc gcgcaaacgc gcatcggcaa ggcggatgcc
     1741 cgccgtctgc aatttggacg gctcggcgtg gctccggatg agttcgtata aaaccagcca
     1801 ttggcggata ttgagcgttt gcagccattt gtcgtcgttt tcggaatgga aaagataaag
     1861 gaagacttcg cccaagttgg caaaatcttt atatgacggg ctgaagcgtt cgtagatgcg
     1921 tatgcccatt tcccgggcaa agctgtggcg cgagaagatg ccgagtttga tgagtgccgg
     1981 ataaatgtgt atttgcgcga gccagatgta aaaacaccgg ctgaagcggg acagcaattc
     2041 cctgtcttgt ttgaatgtgt cgataatcag gtcgaaacgt tcccccgcac attttttgcc
     2101 gccacggcgc aaaaatttaa tcagtgcgtt gaggacgttg acaaagtcgg tatgtcccaa
     2161 gctttccgaa agcagggggc gcaggttttg aggggtaatt ttcttcatat cagccattgt
     2221 accgcatcgg caatccggcg ttaaacgccc cgatggcggg gagggcgcgc ctgcggaagg
     2281 cggcgcattt tccgccggtt tgaaatcaaa ctatattaat gatataataa atatcgataa
     2341 atcaatctga tagaataatc cgtattgatt tttaattttt tcgattataa caatttcggc
     2401 cggtttgtgc atttcgtcat ccggaagccg gtattttgcc ggcaggtttt gaccgctttg
     2461 cccgatgacc gggtttgtct gtttttaaca aaggagtgag aaggtttgtc cctgtctgaa
     2521 tttatagaac gccgaacgtc atttaatctg atggttattt tgacgacttt gttttttgtg
     2581 tgtgttttgg tggtattggt tttaaccgtg ccggatcagg tgcagatgtg gctcgatcgg
     2641 gcaaaagaaa tcatttttac cgagttcagc tggttttatg ttttaacgtt ttccattttt
     2701 ctgggtttcc tgctgatact ctcggtcagc agtttgggaa acatcaggct cggacgggat
     2761 gaagatgtgc cggaattcgg cttcctgtcg tggctggcga tgctgtttgc ggccgggatg
     2821 ggcgtgggtc tgatgttttt cggcgtggca gagccgttga tgcattattt ttcggacatt
     2881 acggccggca cgccggaaca caggcagcag caggcattgc tgcacacggt gttccattgg
     2941 ggcgttcacg cttggtcggt gtacggtacg attgcattgg ctttggctta tttcggtttc
     3001 cgctacaagc tgccgcttgc cctgcgttct tgtttttacc ccctgttgaa agaaaaaatt
     3061 tccggaaggt tcggcgatgc cattgatatt atggcgttgc ttgctacttt tttcggcatc
     3121 atcaccacat tggggttcgg ggcttcgcaa ctgggcgccg gattgcagga aatgggctgg
     3181 attgccgaaa acagcttcag cgtgcaggtt ttgattatcg ccgccgtcat gtccctcgcc
     3241 gtcgtttcgg caatatccgg cgtggggaag ggcgtgaagg tgttgagcga gttgaacctg
     3301 agccttgcgt ttttgctgct gttttttgtt ttggcggcgg gtcccactgt ttacctgttg
     3361 tcggcattcg gcgacaacat agggaactac ctcggaaatc tggtgcgcct cagttttaaa
     3421 acttatgcgt acgaacggga acacaagccg tggtttgaat cttggacggt gctttattgg
     3481 gcgtggtggt gttcttgggc gccgtttgtg ggtttgttta tcgcgcgcat ttcaaagggg
     3541 cgcaccatcc gcgagtttgt cttcggggtt ttgctcatcc ccggcctgtt cggcgttttg
     3601 tggtttaccg tcttcggcaa tacggcgatt tggctgaatg acggggttgc ggggggaatg
     3661 ctcgaaaaga tgacctcctc tccggaaacg ctgcttttta aattctttaa ttacctcccc
     3721 ctgcccgaat tgacgagcat cgtcagcctg ctggtcattt ctctgttttt tgtaacttct
     3781 gccgattccg ggatttatgt cctgaacaat attacctctc gggacaaagg cttgagcgcg
     3841 ccacggtggc aggcggttat gtggggcgtg ctgatgtctg ccgttgccgt tttgctgatg
     3901 cgctcgggcg gactcggcaa cctgcagtct atgaccctga ttgtttccct gccgtttgcc
     3961 ctgctgatgc tgataatgtg tttcagcctg tggaaaggct tgagtgcgga taagaaatat
     4021 tttgagaccc gggttaaccc taccagtgta ttttggacgg gcggcaagtg gaaagaacgg
     4081 ctggtgcaga taatgagcca gacgcaggag caggatattt taaaattcct caaacatacc
     4141 gcatcgcccg ctatgcacga gttgcaacgg gagctttcgg aagaatacgg cttgagcgtc
     4201 cgggtcgata agatgtttca tcaggacgag cccgcaatcg agttcgtcat tcggaaagag
     4261 acgatgcgcg attttatgta cgggattaag tctgtcgggc aggatgtatc cgaccagttg
     4321 attaacgacg gcaagctgcc gcatatccgg catcagacaa cttacaaacc ctacgcttat
     4381 tttttcgacg ggcgcgtcgg gtacgatgtg cagtatatga acaaggacga gctgattgcc
     4441 gacattttga aaaactacga acgttatttg atgttgttgg atgatgtcgg tcaggaactg
     4501 atggcgcacg agcaggtgga attggcagag taagtgcctt atctctggcg tttgccaaaa
     4561 aaaatgcggt gaatcagatt caccgcattt tttttgccgg acggggcttc ggacggcacg
     4621 gcgttcattt gtttccgtca aactgctcct tcaacacccg tttcaatacc ttgcccgtag
     4681 cgttgcgcgg cagcccgtct ttaaagtgga tttgtttggg gattttgaaa tttgccagca
     4741 cggtacgcag gtggcggcgg atttcgttct cgcccaaatc cataccttcc ttcaattgga
     4801 cgaaggcgac gatttcctcg tcggcataac ggtctttcac gccgatgacg gcggcggctt
     4861 cgacggcatc gagtttgtag atttcttctt cgatttcgcg cggatagacg ttttgacctt
     4921 tggaaataat caaatctttt ttgcggtcga cgataaagat aaagccgtct tcgtctatgg
     4981 taacgaaatc gcccgttttc aaccagccgt tgacgatggt ttcatcggtg gcggcaggca
     5041 tattgaggta gccccgcatc accgaaccgc ccctgacgat cagttcgccc acttcgccgc
     5101 gcggcacttc gaccaattct tcatcgacgg ctttggcttc caaaccgggc agggggatgc
     5161 cgacgctgcg ggctttttgc ctctcgggcg tattgacggc gacgacgggc gaggcttcgc
     5221 tcagtccgta gccttccagc aatttggcgc gggggaactt ggctttgaaa tcgaggatgg
     5281 tttgttccgc caaaggtgct ccgccgctga taaacaggcg gatgcggttg aaccatctga
     5341 aataccaagg gattttcgtc ttgctcatcg cggtgtaaat cgcgggcacg cccaaaaaca
     5401 cggtcgcgcg tttgagcagt gcctgtttca aaacgttgga aaaggggaaa acggatttga
     5461 ccaaaataat cgaacacgcc atataaatcg gcagcagcac catagccgtc agcgtgaagc
     5521 tgtggaacat cggcaggaaa acgataaagc ggtcgcgctt ggagatttta aagatgcgtt
     5581 cgatgccgtt caggttggcg aacaggttgg cgtagctgat tagcgcacct ttgggatgcc
     5641 ccgtcgtgcc ggaggtgtag atgatgtgtg ccaaatcatt tatccggggt tggcggccca
     5701 agtcgggttt ttcggggaag cggcgcacgt cttcaaaaaa ggcatcgcct tccgccattt
     5761 cgccgtccgg acggctttgg cccgtccaaa tgattttttc gacgggcgtt tgcgccttca
     5821 agcccgccaa ttcttttgac aggccggccg aggcgaacag gaagcgcgcc ttgcagtcgt
     5881 tcaggatata cgcgtattcg ctgtttttca aaaatgtgtt catcggtacg gcaaccgcgc
     5941 cgatggcgga tacggcgaaa taggcggtaa taaattccgt ggaattggaa accgccagcg
     6001 cgaccgtgtc gccgaacttc acgccgatat tttgcagata cgccgcaacg gcttcggctt
     6061 cctgcttgag cgcgcggtag gcggtttttt ccttgccgtc gaacactgcc gtgccgttgc
     6121 cgtttttgcg gcaggcggcg gtcagcattt cgtagaaatt ggcataagtc cggttcatga
     6181 aacttccttt atggttgcgg acggcgaacg ccctcatttg aaaacaatat gccg
//
LOCUS       DAWFKN010000105         6183 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFKN010000105 DAWFKN010000000
VERSION     DAWFKN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.105"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            24..407
                     /locus_tag="WGR46_001946"
     CDS             24..407
                     /locus_tag="WGR46_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0856816.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            522..995
                     /locus_tag="WGR46_001947"
     CDS             522..995
                     /locus_tag="WGR46_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0856817.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMRIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR46_001948"
     CDS             1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR46_001948"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0856818.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2295..3965
                     /locus_tag="WGR46_001949"
     CDS             2295..3965
                     /locus_tag="WGR46_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0856819.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWKRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4077..5711
                     /locus_tag="WGR46_001950"
     CDS             4077..5711
                     /locus_tag="WGR46_001950"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0856820.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5726..5928
                     /locus_tag="WGR46_001951"
                     /pseudo
     CDS             5726..5928
                     /locus_tag="WGR46_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5939..>6183
                     /locus_tag="WGR46_001952"
     CDS             5939..>6183
                     /locus_tag="WGR46_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856821.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDPVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDSELEHSL"
BASE COUNT         1617 a         1683 c         1548 g         1335 t
ORIGIN      
        1 gagaacattt tgaaaggata ccgatggaac cttcttctta cgcggcagaa aaaaaaggca
       61 aaggcggcat caggcgcgtc attaacgcat tcggctattc gatagacggc atcgccgccg
      121 cctaccgtta cgaagcggca ttccgtcagg ttttgtggct gaacgtactg ctggtgtgcg
      181 cggcattttt ttgggtttcc gaaaccgccg tccgcctgcc gttgattatc gcgtcttttg
      241 tgtcggtcat tgtcgaactg ttcaacaccg ccgtcgaagc tgccgtcgat catacttcga
      301 ccgaaaaaca cgagctggcc aaactcgcca aagacgcagg ttctgccgca caattgttcg
      361 cgatgctgat gttggcggcg gtttggctgt ctgccctgtt cgggtaaaac gcttgcagca
      421 ggatttgtaa tcctttagga ttagtatgtg gtatttatta ttcacttaat ccgctataag
      481 attaagcaca ttccccgtct gatattataa ggagcagaaa gatgaaaaaa ttattggcgg
      541 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgccgc cggagtccac gttgaggacg
      601 gctgggcgcg caccaccgtc gaaggtatga gaataggcgg cgcgttcatg aaaatccaca
      661 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgtgtcg
      721 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc gaaggcggtg
      781 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      841 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      901 acgccaaagc gcaaaccgtc caactggaag tcaaaatcgc gccgatgccg gcaatgaacc
      961 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatgtt tgaaatgccg
     1021 tctgaaaaag cccgggcttt cagacggcat ttttatgccc gcctttaaaa tgtgttaaaa
     1081 tccgccttta aaaaccgccg tttccaacca tattctacgc atgaatacga cagcaaacac
     1141 caccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
     1201 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
     1261 cgacgaatat tgcagcatca aacaggattc gttcgatgcc atcgccgttg ccgatattat
     1321 cggcatcgat atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
     1381 ttttcttcag gaatacagtg cggggcgcac gccgaatccg gatgtgttgt gcaacgccga
     1441 aatcaaattc aaatgctttt tggactacgc cgtagggcag ggcgcggata ccatcgccac
     1501 cggacactat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1561 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgagcgcgc
     1621 gatttttccg ttgggcggtt tggaaaaacc cgaagtgcgc cgccttgccg ccgaatttaa
     1681 tttgccgact gccgctaaaa aagacagcac gggcatctgt ttcatcggcg aacgcccgtt
     1741 tcgcgagttt ctgcagaaat acctgccgac cgacaatggt aaaatggtta cgcccgaagg
     1801 gaaaaccgtc ggcgaacacg tcgggctgat gttctacacg ctcggacagc gcaaaggttt
     1861 gggcattggc ggcgcgggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
     1921 actgattgtc gtgcaaggac acgaccatcc gctgctttat acccgcagcc ttgtgatgaa
     1981 cgatttgagt ttcacgctgc ccgaacgccc gaaggcagga cgctatacct gcaaaacgcg
     2041 ttaccgtatg gcggacgcgc cttgtgagtt gcgctatttg gatgatgaaa ccgccgagct
     2101 ggtgtttgac gaaccgcaat gggcggttac gccgggtcag tccgccgtgc tgtacgacgg
     2161 cgatatctgt ttgggcggcg gtatcatcca aacgaccgac aaacccgtca tcatcacacg
     2221 ataaaggtaa tgccgtctga aacggttttc agacggcatt gttccgctca attccacttt
     2281 aaagaccgat acctatgaaa aaaatttggt tagacagcta cgagaagggc gtcagtgccg
     2341 aaatcgacat cacgcaatac aattccgtca gcgacgtatt ccgccaaagc gtggaaaaat
     2401 ttgccgacca acctgctttt caaaacatgg gtaagacgct gacttatgcc gaaaccggca
     2461 aactggcaac tgatttcgcc tcttatctgc aaaacgtcct caagctgccg cgcggcgaac
     2521 gtgttgccat tatgctgccc aacgtattgc aatacccgat tgcccttttc ggtattttgc
     2581 aggcaggttt ggtggcggtg aacaccaatc cgctctatac accgcgcgag ttggagcatc
     2641 agttgaaaga cagcggtgcg accgccatca tcgttttgga aaatttcgcc aacacgctgg
     2701 agctggtgct gccgcgcacg cagatcaaac acgtcatcgt cgcctccgtc ggcgaaatgt
     2761 tcgggctgct taaaggttcg ctgatcaatt tcatcatccg gaaaatcaag aaaatggtgc
     2821 ccgaataccg tattcgggaa accgtttcct ttcagacggc attgaaagaa ggggcgaagc
     2881 acgttttcca acctgtcgca ttaaaccgcg aagataccgc gctgttgcaa tacacgggcg
     2941 gcacgacagg tgttgccaaa ggggcggtgc tgagtcacgg caacatctgc gccaatatgc
     3001 ttcaggcaaa agaatggatt aaaaaccagt tgcgcgaggg caaagaaacc gttatcgccg
     3061 ccttgccgct gtaccatatt ttcgccttaa ccgtgaatct gatgattttt gccaatgccg
     3121 gctcgaaaat catcctgatt accaacccgc gcgatatgaa aggctttatc ggacaactga
     3181 aaaaacagcg ggttaatgta tgtatcggcg tgaacacact gtttaacgca atggttaacc
     3241 gtcctgattt tgccgaagtc gatttttcag gattgcggtt gactttgggc ggcggtatgg
     3301 cgacccaaaa agccgttgcc gaaaaatgga aaaaaatcac cggcacgccc atcgtcgaag
     3361 cctacggtct gaccgaagcc agccccggcg tgtgctgcaa ccccttaaac atcgaatcat
     3421 acagcggcag catcggtttg cccgtctcgt ccaccgaagt cgaactgcgc gacgcaaacg
     3481 gcaaagaagt ccccgttggg cagccgggcg aattgtgggt aaaaggccct caagtgatgc
     3541 aaggctactg gaaacgcccc gaagaaaccg ccaaagccat agacgcgcgc ggctttttgg
     3601 aaaccggcga tattgccgtg atggacgaaa aaggctggtt gaagctggtc gatcgtaaga
     3661 aagacctcgt cgttgtttcc ggattcaatg tttatccgaa cgaaatcgag gaagtcatcg
     3721 cgcatcacgg caaagtcatg gaagtcgcct gtatcggcgt tcccgacgaa aaaaccggag
     3781 aagccctaaa agtattcgtc gtcaaaaaag acccgtcatt gaccaaagaa gaacttatcg
     3841 ccttttgccg caccgaatta accgcatata aagtaccgaa aaatatcgaa ttccgcgacg
     3901 agttgcccaa gtccaacgtc ggcaaaattc tgcgtcgcga gttgcgccaa agtactggga
     3961 aataaagaaa agatgccgtc tgaaaacagc cgtccaccgt tcagacggca tccgcctgtt
     4021 tgccagaacc gcgcgcttca cgttaaaatc acgcattcca atacgggtat ttcattatga
     4081 ccaaattcat tttcgtcacc ggcggcgttg tctcctcact gggtaaaggt atcgccgccg
     4141 cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt taccatgctc aagctcgatc
     4201 cttatatcaa cgtcgatccc ggcacgatga gcccgttcca acacggcgaa gtgttcgtaa
     4261 ccgacgacgg cgcggaaacc gacctcgact tgggacacta cgaacggttt atcgattcca
     4321 cgatgacccg ccgcaacagc ttcagcacgg ggcaggtgta cgaaaacgtc atcgccaaag
     4381 aacgacgggg cgactacctc ggcggcacgg ttcaagtcat cccgcacatt accgacgaaa
     4441 tcaaacgccg catccacgaa ggcgcggcag gttacgatgt ggcgattgtc gaaatcggcg
     4501 gcacggtcgg cgacatcgaa tcgctgccgt ttttggaagc catccgccag atgcgaagcc
     4561 agttgggacg caacaacacc ctgttcgccc acttgagcta cgtcccctac atcgccgctg
     4621 caggcgaaat caaaaccaag ccgacccagc acaccgttaa agaaatgttg agcatcggtt
     4681 tgcaacccga catcctgatt tgccgtatgg acaggacaat gcctgcggac gaacgccgca
     4741 aaatcgcctt gttctgcaac gtggaagaac gcgcgattgt cggcagctac gatgtggaca
     4801 gcatctacga atgccccgaa atgctgcacg accaaggcat cgacaacatc attaccgagc
     4861 agttgcagct taatgtgcaa caggcggatt tgaccgcgtg gaaaaaaatc gtccacgcca
     4921 tccaaaaccc gaaacacacc gtcaaaatcg ccatggtcgg caaatacgtc gatttgaccg
     4981 agtcctacaa atcattgatc gaagccttga aacacgcagg cattcacacc gaaaccgacg
     5041 tacaaattac ctttgtcgat agcgaaagca tcgagaaaaa caacggcgac gtttctatgc
     5101 tcaaagatat ggatgccatc ctcgttcccg gcggtttcgg ttcgcgcggc gtggaaggca
     5161 aaatcgccgc tgtgcgctat gcccgtgaaa acaacgtgcc atacttgggt atctgcctcg
     5221 gtatgcagat tgcgctgatt gaatacgccc gcgacgtggc aggtttgaaa ggtgcgaatt
     5281 ccactgagtt cgacctcaaa tgcgccgccc ccgtcgttgc cctgattgac gaatggcaaa
     5341 ctgccgacgg cagcgtcgaa acccgcgatg aatccgccga tttgggcggc acgatgcgtt
     5401 tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc cgccaaaatc tacggcagcg
     5461 gacatatccg cgaacgccac cgccaccgct acgaagtcaa caacaactat gttcctcagt
     5521 tggaaaaagc aggcttggtt atcggcggcg tatccgccgg acgcgaacgc ttggtcgaaa
     5581 ccatcgagct gccgaaccac ccttggttct tcgcctgcca gttccatccc gaattcactt
     5641 ccaacccgcg caaagggcat cccttgttca ccgcgtttgt caaagctgcg ttgaacaata
     5701 aaaaagcctg ataaagcgtt acttgatgat caaaatgccg tctgaaagcc tgaatgaaga
     5761 ctttcagacg gcattttcgc aaatcgggga ttataaaagc catcaattta atttttatgg
     5821 ttttatctaa tatttaccaa ctgaatattg ttaagcgggg cgtgaaactc aagttgagac
     5881 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttagga gatgttccat
     5941 gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg accgtttccc
     6001 gctattgaag ttggatccag tgattgattg gcagccgatc gaacagtacc tgaatcgtca
     6061 aagaacccgt taccttagag accaccgcgg ccgtcccgcc tatcccctgt tgtccatgtt
     6121 caaagccgtc ctgctcggac aatggcacag cctctccgat tccgaactcg aacacagcct
     6181 cat
//
LOCUS       DAWFKN010000106         6017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFKN010000106 DAWFKN010000000
VERSION     DAWFKN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.106"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            262..1209
                     /locus_tag="WGR46_001953"
     CDS             262..1209
                     /locus_tag="WGR46_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0856822.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAIAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFEHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1362..2486)
                     /gene="rluD"
                     /locus_tag="WGR46_001954"
     CDS             complement(1362..2486)
                     /gene="rluD"
                     /locus_tag="WGR46_001954"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0856823.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2485..3288
                     /locus_tag="WGR46_001955"
     CDS             2485..3288
                     /locus_tag="WGR46_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0856824.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3346..5565)
                     /locus_tag="WGR46_001956"
     CDS             complement(3346..5565)
                     /locus_tag="WGR46_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0856825.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLATFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1487 a         1891 c         1445 g         1194 t
ORIGIN      
        1 aacaggggat ggcacatcaa gcaccgcact gcgtgttgcc cgaacgagac taccctccga
       61 ttagattcta tcgctataaa cagacggatt tcaaccggaa aggaatgggg ataaagtccg
      121 tttccgacac ctctcgggcg ataccgtctg aaaaccaatc tccactttca gacggcattg
      181 tttaggcacg gtcattttgc aaattaaggt taaggcgtgt atgcttgcct tcccttaaaa
      241 acaaacacgc aacgcattat tatgaatatc ctcagtaaaa tcagcagctt tatcggaaaa
      301 acattttccc tctgggccgc gctctttgcc gccatcgctt ttttcgcgcc cgacaccttc
      361 aaatgggcgg ggccttatat tccttggctg ttgggcatta ttatgttcgg tatgggtttg
      421 acgctcaaac cttccgactt cgatattttg ttcgaacatc ccaaagtcgt catcatcggc
      481 gtaatcgcac aattcgccat tatgccggca accgcctggc tgctgtccaa actgttgaac
      541 ctgcctgccg aaatcgcggt cggcgtgatt ttggtcggct gctgcccggg cggtacggct
      601 tccaatgtga tgacctatct ggcgcgcggc aatgtggctt tgtcggttgc cgttacgtct
      661 gtttccaccc tgatttcccc attgctgact cccgccatct tcctgatgct tgccggtgaa
      721 atgctggaaa tccaagcggg cagtatgttg atgtccatcg tcaaaatggt tttgctcccc
      781 attgttttgg gtttgattgt ccataaggtt ttgggcagta aaaccgaaaa gctgaccgat
      841 gcgctgccgc tggtttccgt tgccgccatc gtgctgatta tcggcgcggt tgttggggca
      901 agcaaaggca agattatgga aagcggcctg ctgatttttg cggttgtcgt actccacaac
      961 ggcatcggct acctgctcgg cttctttgcc gccaaatgga ccggcctgcc ttatgatgca
     1021 caaaaaacgc tgaccatcga agtcggtatg caaaactcgg gcctggccgc cgcgcttgcc
     1081 gccgcacact ttgccgccgc gccggtcgtt gccgttccgg gcgcattgtt cagcgtgtgg
     1141 cacaatatct ccggctcgct gctggcaact tattgggcgg ccaaagccgg taaacataaa
     1201 aaaccctaaa cgcctttcat cataatgccg tctgaacggg gcaaacaaaa ctggcggttt
     1261 ctgcccggcc ccttcagacg gcctttgttt gaaatcggcg gggacgattg cccggctgct
     1321 ccgattcggc tgcctgcgtt cgccccgcct ttcaagccgc ctcactccct tacataaacc
     1381 acttcgacat cgtagtcgtc ttcgttccaa tcgtcatcgt cgtccgcgcc gaatttgtcc
     1441 tgccattcct cttcattgct caaagacgaa tccaaaccgg cttcaagacg gaggacagac
     1501 aacaaatgat atatgtcgtc cggaatcggt gcttcaaacg aaacggtttc gccggttttc
     1561 ggatgagtga aacttaagcg gtaggcgtgc aatgcctgac gcgcacccaa acttttaacg
     1621 gcttctttca ccgtgtcgcc gcacggatgg cgcgggttgc cgtaaaccgg gtcggcggca
     1681 agcggatggt tggcctcacg catatggacg cggatttggt gcgtcctgcc tgtttcgagc
     1741 gagcattcga tatagctgtg cgccagatag cgttccaaca ctttgacgtg ggtaacggct
     1801 ggtttgccgc cgaatttgac gactgccatt ttcaggcggt tgtgcggatc gcgtccgatt
     1861 tgggtttcga ttttaccgtc gaaggggacg atgccattgg cgacggcgcg gtagatgcgt
     1921 ttgaccgtgc gctcttgaag ctgcctcacg agggaatttt gcgccgacag ggttttggca
     1981 accaccatca gcccgctggt ttctttgtcc aaacggtgta cgatgcccgc gcgcggaatt
     2041 tgggacagct cggggcaatg cgccaacagg ccgttgagca gcgtccccgt ccagttgccc
     2101 gccgccggat gcaccaccag tccggccggt ttgttgacga cgatgacggt atcgtcttcg
     2161 taaacgatat ccaaagccat aggctctgga acaaacgcca gattttcctc actcggacgg
     2221 acggttacac aaatttgctc gccgcctatc attttgtctt tgggttgcga aggtttatcg
     2281 tttacaataa ccgcgccttc tttaatccat gatgtcaggc ggctgcgcga gtagtcgggc
     2341 agaagtttcg ccaacaccgc atccaaccgc ccgccggcaa gctcgagcgg aacggtcaga
     2401 ccgatacggt tttcagtttc ggaagctgac gcaaagtctg aatcatcgct ataatcggat
     2461 tcattatcaa aggaagtatt ctgcatgaaa aaaattcttt taacggtttc attaggtttg
     2521 gcactgagtg cctgtgccac tcaaggtacg gtcgataaag atgctcagat tacccaagat
     2581 tggagtgtgg agaagctcta tgccgaagcc caggacgagc tgaacagcag caattatacg
     2641 cgagctgtca aattatacga aatcttggaa tcgcgcttcc ctaccagccg ccatgcccgg
     2701 caatcccaac tggataccgc atacgcctat tataaagacg atgaaaaaga caaagctctg
     2761 gcggcaatcg aacgcttccg ccgccttcac ccgcaacacc cgaatatgga ttacgcgctg
     2821 tatctgcgcg gcttggtgct gttcaacgaa gaccagtcct tcttgaacaa actggcctcg
     2881 caagactggt ccgaccgcga cccgaaagcc aaccgcgaag cgtaccaggc gtttgcggaa
     2941 ctcgtccaac gcttccccaa cagcaaatac gccgccgatg caaccgcacg catggtcaaa
     3001 ctggtcgatg cactgggcgg caatgaaatg tcggtggcgc gctactacat gaaacgcggt
     3061 gcatacatcg ccgccgccaa ccgcgcccaa aaaatcatcg gcagctacca gaatacgcgt
     3121 tatgtcgaag aatcgctcgc catattggaa cttgcctacc aaaaactcgg caaaccacag
     3181 cttgccgccg atacgcgccg cgtgttggaa accaacttcc cgaaaagccc gtttttgacg
     3241 cacgcttggc agcccgacga tatgccctgg tggcgttact ggcattaagc ccatcttcgt
     3301 aatgccgtct gaagccgttt cagacggcat tttatgtttc aaacctcatt caaacggttt
     3361 tttctgccaa tagaatttat accctctcaa acgttgagcc ttcacgccgc cgcgtcccaa
     3421 gccgaattgc agtgcgcccg acaaatcggt acgcagcagt ttgatgccgt gtgcgcggac
     3481 acggttctgc accgcttcgg tcggatgttt gtaggcgttg gcataaccgc ttgaagcaac
     3541 ggcatattcg ggcgagacgg cattgaggaa cacgcccgac gaggacgtat tgctgccgtg
     3601 atgtcccaac accaaaacct ggctgtacag gttgcctcca tacttgccga ccaggctttc
     3661 ctcgcccttc gtatccaaat cgcccgttac cagcagtgcc gcaccgcccg ccacaacacg
     3721 caaaacacaa cttttcccat tatcatcgat gtttttgcgt tcagacggca tcaaaaactc
     3781 aaaatccacg ccgtcccatt gccaacgctg ttccgcacaa tgccgcgccc cctcataaaa
     3841 ttccggctgt ccggcataaa tcccgccgtt cggtatcttg ccgactgcct gaaaaccgcc
     3901 gtcgtggtcg ctgtcgtgat gcgacagaac cagcttgtcc aaacggcgga cacccgccgc
     3961 attcaaactc ggcacaatcc ccatctgcgc cgccgctgcc gttccggtgt caaacaaaag
     4021 atgatgattt gccgtctgaa ccgacaccga caaaccctgc cccgcatccc aaaccgtaac
     4081 cgcaacctca ttttccggca cgccgggtga acggtaaaac acaaaccctg ccaacagcaa
     4141 caccgcccac ggacgcaaac ccaagccgcg cggcagcagc aacagcaaag cggcacacac
     4201 cgccaacacc aacagcggca aaggtgcgga ggcaacggca aactcgggcg acacatcggc
     4261 aagccacacc aaaaaccgca aagtatattc cgccaaaaat gcccccaact gttgcaaagg
     4321 cgcaaacggc acgaccgaac ccagcaacgc cagcggcgtc aatacccaag aaaaccacgg
     4381 aattgccacc gcattgacca aagggctgac caaaggcagc gaagcaaaca gataaccgag
     4441 caaaaccagc gacaacaccg aagccgccca ctgcccgcgc aaagcagttt gccgttttcc
     4501 ctcgtgcaaa cgccccgagc acgcccatat cagggccgcc accaaaccga aagacagcca
     4561 agtccccaca cccaagaccg ccgacgggtc gaacagcagc accgccgcca acgcctgcca
     4621 ccacgtcgcc cacgccgaca atcttcccct gcgccaagcc cacgcaaacg tcgccaacat
     4681 caaaacgctg cgctgcgtcg gcacggaaaa accggcaagc agcgcgtaaa acagcgcgcc
     4741 tgcacacccc gccgccaaaa cccacgcgcg cggccgggca ggaaggcgcg gggaacaggc
     4801 aagcagccgc ttcgccagcc acgcaaacat taccgccacc atcgtaacgt gcaaacccga
     4861 aatactgacc aaatgcgtca gccccaacgg ccggaacgcc tgccacaatt cggggcgcaa
     4921 tgccgactgt tcgcccacgc tcaacgcgcg catcagcccg atgccgtctg aaagcccgcc
     4981 gtccgcatcc gcctgctgcc aattacggct gatgcggctg cgccaaaccg caatccccca
     5041 accgcttccg ccatgcagca aaaccctgtc cgcaccgacc gtccccgcgc cgcctattcc
     5101 gttggataat gcccacgcct cacggttcaa accgcgcagg ttcaactccc cgacgacagg
     5161 gtgcacacgt gccgttatcc gccatctgct cccgaccgcc cattcgcgcc gtttgtagtc
     5221 cgacagcagc aaatcaaacg tccgaccacc gctgtccaca gcctttgccg caaactgcac
     5281 acgccgcccg tccgacctcg gcatatccgc cacttccacc gtcaaaggca cgcctgaaac
     5341 ggcttctgcc caccattggg aagacaatgc cgcctccgtc ctgaatacgc cgtaagccgc
     5401 gcccgccaac acgcacagca tcagaccggc aaacgcaaaa cgccttgcaa acacagcaaa
     5461 aaccgcaaaa gccgccagcc aaaacagcca atgcggcacg accggcagcg cgaacgaagc
     5521 cgccgcaccg acaacccaac acggcaaaaa ccaacgacgg aacacgtctc cccaccccag
     5581 atatttcaaa aataaacgga taataaagca aatccgaaaa accgtcataa caagatttga
     5641 aaatgatata gtggtttaaa tttaaatcag gacaaggcga cgaagccgca gacagtacaa
     5701 gcagtacgac aaggcgaggc aacgctgtac tggtttttgt taaaccacta taagaatgca
     5761 aacgaaaagc ctcagtatta tccgagacaa cggcaaactt tccatcgtca ttcccgcaca
     5821 agcgggaatc caaaaacaaa aagccgcaga aatttaacgg aagaaccaaa ccccgaaaac
     5881 cggctccccg ccttcgcttg aataacagaa caaaggaatc agtattatcc gagacaaccg
     5941 caaactttcc atcgtcattc ccgcacaagc gggaatccaa acttgtccgc acggaaactt
     6001 atcggataaa acggttt
//
LOCUS       DAWFKN010000107         5896 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFKN010000107 DAWFKN010000000
VERSION     DAWFKN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.107"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..317)
                     /locus_tag="WGR46_001957"
     CDS             complement(<1..317)
                     /locus_tag="WGR46_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0856826.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRA"
     gene            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGR46_001958"
     rRNA            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGR46_001958"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(563..3454)
                     /locus_tag="WGR46_001959"
     rRNA            complement(563..3454)
                     /locus_tag="WGR46_001959"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3861..3936)
                     /locus_tag="WGR46_001960"
     tRNA            complement(3861..3936)
                     /locus_tag="WGR46_001960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3901..3903),aa:Ala,seq:tgc)
     gene            complement(3942..4018)
                     /locus_tag="WGR46_001961"
     tRNA            complement(3942..4018)
                     /locus_tag="WGR46_001961"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3982..3984),aa:Ile,seq:gat)
     gene            complement(4120..5660)
                     /locus_tag="WGR46_001962"
     rRNA            complement(4120..5660)
                     /locus_tag="WGR46_001962"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1280 a         1725 c         1262 g         1629 t
ORIGIN      
        1 tgcgcacggt attccgcaat ggccttcgcc ttcgcagggc ctatgcccgg cagcgcctcc
       61 agctcctgct gcgaagccgc attgatgttt accgccgcaa gggagaaggc gcaggagaac
      121 agcatacaga acagcacgaa cattttcttc atggtttttc ctttaagggt tgcaaacaat
      181 aaaccgcatc ttgcgacgat aaaacgggtc attctaaaat gaatatccca aagtttcaag
      241 ccgttcctcc gcaaacccga ccggacgccg tacggatgcc gtcccgccat ccccgacatt
      301 ttttccgggc aaagcaaaaa cccccgaata atcgggggtt ttctgaatgg gtgtttggca
      361 gtgacctact ttcgcatgga agaaccacac tatcatcggc gctgagtcgt ttcacggtcc
      421 tgttcgggat gggaaggcgt gggaccaact cgctatggcc gccaaactta aactgttaca
      481 aatcggtaaa gccttaatca atatattcgg taatgactga atcagtcagt aagcttttat
      541 ctcttgaagt tcttcaaatg atagagtcaa gcctcacgag caattagtat gggttagctt
      601 cacgcgttac cgcgcttcca caccccacct atcaacgtcc tggtctcgaa cgactcttta
      661 gtgcggttaa accgcaaggg aagtctcatc ttcaggcgag tttcgcgctt agatgctttc
      721 agcgcttatc tcttccgaac ttagctaccc ggctatgcaa ctggcgttac aaccggtaca
      781 ccagaggttc gtccactccg gtcctctcgt actaggagca gcccccgtca aacttccaac
      841 gcccactgca gatagggacc aaactgtctc acgacgtttt aaacccagct cacgtaccac
      901 tttaaatggc gaacagccat acccttggga ccgactacag ccccaggatg tgatgagccg
      961 acatcgaggt gccaaactcc gccgtcgata tgaactcttg ggcggaatca gcctgttatc
     1021 cccggagtac cttttatccg ttgagcgatg gcccttccat acagaaccac cggatcacta
     1081 tgtcctgctt tcgcacctgc tcgacttgtc ggtctcgcag ttaagctacc ttttgccatt
     1141 gcactatcag tccgatttcc gaccggacct aggtaacctt cgaactcctc cgttacgctt
     1201 tgggaggaga ccgccccagt caaactgcct accatgcacg gtccccgacc cggatgacgg
     1261 gtctgggtta gaacctcaaa gacaccaggg tggtatttca aggacggctc cacagagact
     1321 ggcgtctctg cttctaagcc tcccacctat cctacacaag tgacttcaaa gtccaatgca
     1381 aagctacagt aaaggttcac ggggtctttc cgtctagcag cgggtagatt gcatcttcac
     1441 aaccacttca acttcgctga gtctcaggag gagacagtgt ggccatcgtt acgccattcg
     1501 tgcgggtcgg aacttacccg acaaggaatt tcgctacctt aggaccgtta tagttacggc
     1561 cgccgtttac tggggcttcg atccgatgct ctcacatctt caattaacct tccagcaccg
     1621 ggcaggcgtc acaccctata cgtccacttt cgtgttagca gagtgctgtg tttttaataa
     1681 acagtcgcag ccacctattc tctgcgaccc tccggggctt acggagcaag tccttaacct
     1741 tagagggcat accttctccc gaagttacgg tatcaatttg ccgagttcct tctcctgagt
     1801 tctctcaagc gccttagaat tctcatcctg cccacctgtg tcggtttgcg gtacggttcg
     1861 attcaaactg aagcttagtg gcttttcctg gaagcgtggt atcggttgct tcgtgtccgt
     1921 agacactcgt cgtcacttct cggtgttaag aagacccgga tttgcctaag tcttccacct
     1981 accggcttaa acaagctatt ccaacagctt gccaacctaa ccttctccgt ccccacatcg
     2041 catttgaatc aagtacagga atattaacct gtttcccatc gactacgcat ttctgcctcg
     2101 ccttaggggc cgactcaccc tacgccgatg aacgttgcgc aggaaacctt gggctttcgg
     2161 cgagcgggct tttcacccgc tttatcgcta ctcatgtcaa cattcgcact tctgatacct
     2221 ccagcacact ttacaatgca ccttcatcgg cctacagaac gctcccctac catgccggta
     2281 aaccggcatc cgcagcttcg gttatagatt tgagccccgt tacatcttcc gcgcaggacg
     2341 actcgaccag tgagctatta cgctttcttt aaatgatggc tgcttctaag ccaacatcct
     2401 ggctgtctgg gccttcccac ttcgtttacc acttaatcta tcattgggga ccttagctgg
     2461 cggtctgggt tgtttccctc ttgacaacgg acgttagcac ccgctgtctg tctcccgagg
     2521 aaccacttga tggtattctg agtttgccat gggttggtaa gttgcaataa ccccctagcc
     2581 ataacagtgc tttaccccca tcagtgtctt gctcgaggca ctacctaaat agttttcggg
     2641 gagaaccagc tatctccgag tttgtttagc ctttcacccc tatccacagc tcatccccgc
     2701 attttgcaac atgcgtgggt tcggtcctcc agtacctgtt acggcacctt caacctggcc
     2761 atggatagat cactcggttt cgggtctaca cccagcaact catcgcccta ttaagactcg
     2821 gtttccctac gcctccccta ttcggttaag ctcgctactg aatgtaagtc gttgacccat
     2881 tatacaaaag gtacgcagtc acacctctag ggcgctccca ctgtttgtat gcatcaggtt
     2941 tcaggttctg tttcactccc ctcccggggt tcttttcgcc tttccctcac ggtactggtt
     3001 cactatcggt cgatgatgag tatttagcct tggaggatgg tccccccata ttcagacagg
     3061 atttcacgtg tcccgcccta cttttcgtac gcttagtacc actattgaga tttcgaatac
     3121 gggactatca cccactatgg tcaagcttcc cagcttgttc ttctatctcg acagttatca
     3181 cgtacaggct cctccgcgtt cgctcgccac tacttgcgga atctcggttg atttcttttc
     3241 ctccgggtac ttagatggtt cagttctccg ggttcgcttc tctaagtcta tgtattcaac
     3301 ttaggatact gcacagaatg cagtgggttt ccccattcgg acatcgcgga atcattgctt
     3361 tattgccagc tcccccgcgc ttttcgcagg cttacacgtc cttcgtcgcc tatcatcgcc
     3421 aaggcatcca cctgatgcac ttattcactt gactctatca tttcaagaac ttctttgact
     3481 ttgcctaaca ttccgttgac tagaacatca gacttgaatt tcctactttg ataaagctta
     3541 ctgctttgtt gtgtcttaat cctgcctttt gtgtttcagg attaagtcga tacaatcatc
     3601 acccaaatac tgtgtttgtt ttcttttctc ttgtgagcga ttttttatcc tttgcaaaga
     3661 ataaaaaatc aaaacaaacg ctttgtcttt gtttgttgat ttcggctttc caatttgtta
     3721 aagatcgatg cgttcgatat tgctatctac tgtgcaaatc aaaacgagct gattattata
     3781 tcagcatttt gttcttggtc aagtgtgacg tcgccctgaa tggattctgt tccattcttc
     3841 cgttttgatt tgtacagtat tggtggaggc aaacgggatc gaaccgatga ccccctgctt
     3901 gcaaagcagg tgctctacca actgagctat gcccccgttc ttggtgggtc tgggaggact
     3961 tgaacctccg accccacgct tatcaagcgt gtgctctaac cagctgagct acaaacccgg
     4021 attctcttct taagcgaatc ttgccttcac tcaagcttct tccgcatctt tttcagttta
     4081 ccgataagtg tgaatgccta aagcctcttc tttctctaga aaggaggtga tccagccgca
     4141 ggttccccta cggctacctt gttacgactt caccccagtc atgaagcata ccgtggtaag
     4201 cgggctcctt gcggttaccc tacctacttc tggtatcccc cactcccatg gtgtgacggg
     4261 cggtgtgtac aagacccggg aacgtattca ccgcagtatg ctgacctgcg attactagcg
     4321 attccgactt catgcactcg agttgcagag tgcaatccgg actacgatcg gttttgtgag
     4381 attggctccg cctcgcggct tggctaccct ctgtaccgac cattgtatga cgtgtgaagc
     4441 cctggtcata agggccatga ggacttgacg tcatccccac cttcctccgg cttgtcaccg
     4501 gcagtctcat tagagtgccc aactgaatga tggcaactaa tgacaagggt tgcgctcgtt
     4561 gcgggactta acccaacatc tcacgacacg agctgacgac agccatgcag cacctgtgtt
     4621 acggctcccg aaggcactcc tccgtctccg gaggattccg tacatgtcaa gaccaggtaa
     4681 ggttcttcgc gttgcatcga attaatccac atcatccacc gcttgtgcgg gtccccgtca
     4741 attcctttga gttttaatct tgcgaccgta ctccccaggc ggtcaatttc acgcgttagc
     4801 tacgctacca agcaatcaag ttgcccaaca gctaattgac atcgtttagg gcgtggacta
     4861 ccagggtatc taatcctgtt tgctacccac gctttcgggc atgaacgtca gtgttatccc
     4921 aggaggctgc cttcgccatc ggtattcctc cacatctcta cgcatttcac tgctacacgt
     4981 ggaattctac ctccctctga cacactcgag tcacccagtt cagaacgcag ttcccaggtt
     5041 gagcccgggg atttcacatc ctgcttaagt aaccgtctgc gcccgcttta cgcccagtaa
     5101 ttccgattaa cgctcgcacc ctacgtatta ccgcggctgc tggcacgtag ttagccggtg
     5161 cttattcttc aggtaccgtc atcagccgct gatattagca acagcctttt cttccctgac
     5221 aaaagtcctt tacaacccga aggccttctt cagacacgcg gcatggctgg atcaggcttg
     5281 cgcccattgt ccaaaattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt
     5341 cccagtgtgg cggatcatcc tctcagaccc gctactgatc gtcgccttgg taggccttta
     5401 ccccaccaac tagctaatca gatatcggcc gctcgaatag cgcaaggccc gaaggtcccc
     5461 tgctttctct ctcaagacgt atgcggtatt agctgatctt tcgatcagtt atcccccact
     5521 actcggtacg ttccgatatg ttactcaccc gttcgccact cgccacccaa gaagcaagct
     5581 tctctgtgct gccgtccgac ttgcatgtgt aaagcatgcc gccagcgttc aatctgagcc
     5641 aggatcaaac tcttatgttc aatctctaac tttttaactt ctggtctgct tcaaagaaac
     5701 caacaggaca atgttcaaaa cattatcttg tctgtctttc aaacagtgtg aggctcaagg
     5761 cactcacact tatcggtaat ctgttttgtt aaagagcgtt tgaattataa agtatttctt
     5821 ccgcctgtca agatatctct cgatatcccc aacattctgt gctatacttt tcagttcgtc
     5881 cgccacttct gcagca
//
LOCUS       DAWFKN010000108         5803 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFKN010000108 DAWFKN010000000
VERSION     DAWFKN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.108"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..574)
                     /locus_tag="WGR46_001963"
     CDS             complement(<1..574)
                     /locus_tag="WGR46_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0856827.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WGR46_001964"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WGR46_001964"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0856828.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVSTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2452..3120
                     /locus_tag="WGR46_001965"
     CDS             2452..3120
                     /locus_tag="WGR46_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0856829.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYTK
                     DQHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            3150..3767
                     /gene="pilV"
                     /locus_tag="WGR46_001966"
     CDS             3150..3767
                     /gene="pilV"
                     /locus_tag="WGR46_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0856830.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFQVGV
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGAAPSLSDSGTFSSNCDNNK
                     ANGDTLIKVLWVNDSAGDSDIAHTNLETNGNNIVYTYQARVGGRE"
     gene            3764..4702
                     /locus_tag="WGR46_001967"
     CDS             3764..4702
                     /locus_tag="WGR46_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0856831.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNRS
                     FSLKRNGIDKLIPIAESSNIGYLGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNEKGE
                     WGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFKYTDKFDSAQNAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            4681..5274
                     /locus_tag="WGR46_001968"
     CDS             4681..5274
                     /locus_tag="WGR46_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0856832.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMKYKKGTRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5279..5752
                     /locus_tag="WGR46_001969"
     CDS             5279..5752
                     /locus_tag="WGR46_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0856833.1"
                     /translation="MEQKGFTLIEVMIVVTILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSDYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSADAGCEAFSNRKK"
BASE COUNT         1572 a         1285 c         1494 g         1452 t
ORIGIN      
        1 caaaacgttt ggcgacttcg tcccagttga cgatttccca gaaacctttc aggtagttgg
       61 gacggctgtt gcggtagtcg atgtaatagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggcgtagcgg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc cacgcccaac cggagccgaa agtaccggca gcgcaagcgg
      241 agaatgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccggcagg tttgccttga cctttgggcg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgggc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagttt ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag ctcggcagcg cacatatttt acctgttttg
      661 agggatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacac agctatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg ctgcctccac attcgatgga ggcggaacaa tccgttttgg gcggtttgat
      841 gctggaaaat ccggcttggg acaggattgc cgatgtggtt tcgggagagg atttctaccg
      901 ccacgaacac cgcctgattt tccgatccat tgccaaatta atcaatgaga gccgtcccgc
      961 cgatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 atttgaatat ctgattacgc tggcgcaaaa caccccgtct gccgccaaca tccgccgcta
     1081 tgccgaaatc gtgcgcgagc gttccatcat gcgccagctc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcctacaatc cgcaaggcag ggacgcgggg cagcttttgg acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac ggccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggtgtgt cgacagggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcaacccggc gacctgatta tcgttgccgg tcgtccgtct atgggtaaga ccgccttttc
     1441 catcaatatc gccgaacacg ttgccgtaga aggcaagctg cccgttgctg ttttctcgat
     1501 ggaaatgggc ggggcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgcctga acgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtctga ccgcgctcga
     1681 actgcgcgcc cgtgcccgcc gtctcgcccg tcaatttaac aataagcttg gattgattgt
     1741 catcgactac ctgcaactga tggcaggatc cggccgttcc gacaaccgag cttcggagct
     1801 gggagagatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ttgagccgca cggtcgaatc gcgtaccgac aaacgcccga tgatgtccga
     1921 ccttcgcgaa tccggtgcta tcgagcagga tgccgacctg attatgttca tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaggat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaaat atagtggatt
     2281 aacaaaaatc aggacaaggc aacgaagccg cagaccgtac aaatagtacg gaaccgattc
     2341 acttggtgct tgagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgccgt
     2401 actggtttga atttaatcca cgatacatta ccagttaacg ttctattgct tatgtgtaca
     2461 cgaaaacaac aaggtttcac gctaacagag ctgctcatcg tgatggtcat tgcagccatt
     2521 atggcgatga tagccctccc caatatgagc caatggattg catcccgccg cattgccagt
     2581 cacgcggagc ggattgccaa ccttttgcgt ttctccaggg gcgaagccgt ccggctcaat
     2641 ctccctgtct atatctgtcc tgttcaagtt aaaaaagacg gtacgcccaa caataaatgt
     2701 gactccggca agaaggggca gggaatgttg gctttcggcg acaaaaacgg caataagaca
     2761 tatgacggcg atacggcgga tgttctcctt cgcagtgtgg tattgaatga tgatatcaat
     2821 gataagcgga ttaattatgc cttcaaccat atcgctttcg gtcagactca gccgaccgct
     2881 gaccgtgtag tttggacatt caatcaaaac gggacgttcg gttatacgaa agaccagcat
     2941 cttacaagca aatccagctt tttttattcc gacggttata tccaaatcgt gctgacagat
     3001 gcgagagcag tttcagatgc cgataagaaa ttccgttcgg cggtggtttt gattaacagc
     3061 agcggcaggg ttgaggtttg tcctagaaac gataggcgta ccgtatgcca atataaatga
     3121 ttgttttagt cggtttggga aataaaaaaa tgaagaataa tgattgcttc cgcctgaaaa
     3181 acccccagtc tggtatggcg ctgatagaag tcttggtcgc tatgctcgtt ctgaccatcg
     3241 gtattttggc actattgtct gttcagttgc ggacagtcgc ttccgtcagg gaggcggaga
     3301 cacaaaccat cgtcagccaa atcacgcaaa acctgatgga aggaatgttg atgaatccga
     3361 ccattgattc ggacagcaac aagaaaaact ataatcttta catgggaaac catcatacac
     3421 tatcagttgt ggatggcgat tttcaggttg gtgtcataaa aactaagacg cagttggcag
     3481 aggcacaatt gaagagattt agttatgagc tgaaaaatgc cttgccggat gcggcagcca
     3541 tccattacgc cgtctgcaag gattcgtcgg gtgctgcgcc gtcattgtcc gacagcggta
     3601 ctttttcttc aaattgcgac aataataagg caaatgggga tactttgatt aaagtattgt
     3661 gggtaaatga ttcggcaggg gattcggata ttgcacatac gaatcttgag acgaacggca
     3721 acaatatcgt atatacctat caggcaaggg tcggaggtcg ggaatgagac gtaaaatgct
     3781 aaacgtacca aaaggcaatt atgatggtat gaagggtttt accattattg aatttttggt
     3841 tgcgggcatg ctcagtatga ttgtcctgat ggcggtcgga tcgagttact tcacgtcccg
     3901 gaaattaaat gatgcggcaa acgagcgtct tgctattcaa caggatttgc ggaatgcggc
     3961 aacattaatt gtccgcgatg caagaatggc agggggcttc ggttgtttca atatgtccga
     4021 gcatcctgca actgatgttg tttccgatgt ggcgcaaaaa aaccgttctt tttccttaaa
     4081 aaggaacggt atagataaac ttattcccat agcggaatct tcaaatatcg gatatctggg
     4141 ttttacccag cgccttaacg cattgatttt ccaatacgga atcgatgatg ttaatgcaag
     4201 cgccgatact accgtcgtca gcagctgtgc caaaatagca aaaccgggta agaaaatatc
     4261 taccttgcaa gaagcaaaga gtgcattaca gattactaat gatgataaac aaaatggaaa
     4321 tatcacccgt caaaggcatg tggtcaatgc ctatgcggtc ggcaggattg ccggtgagga
     4381 aggtttgttc cgcttccaat tgaatgaaaa gggcgagtgg ggtaatcctc agttgttggt
     4441 taaaaagatt aataaaatgg atatacggta tatttatgtt tccggctgtc ctgaagatga
     4501 cgatgccggc aaagaggaaa cattcaaata tacggataaa ttcgacagcg cccaaaatgc
     4561 tgttacgccc gccggggtgg aggttttatt gagtagcggt actgatacca agattgccgc
     4621 ttcttcagac aatcatattt atgcttaccg tatcgatgcg acaatacgcg ggggaaatgt
     4681 atgcgcaaac agaacacttt gacgggaatc ccgacttctg acggacagag ggggttcgcg
     4741 ctgtttatcg tactgatggt gatgatcgtc gtggcttttt tggttgtaac tgccgcgcag
     4801 tcttacaata ccgagcagcg gatcagtgcc aacgaatcag acaggaaatt ggctttgtct
     4861 ttagccgagg cggctttgcg ggaaggcgaa cttcaggttt tggatttgga atatgatacg
     4921 gacagtaagg ttacatttag cgaaaactgt ggaaaaggtc tgtgtaccgc agtgaatgtg
     4981 cggacaaata atgataatga agaggctttt gacaatatcg tggtgcaagg caagcccacc
     5041 gttgaggcgg tgaagcgttc ttgccctgca aattctaccg acctgtgcat tgacaagaaa
     5101 gggatgaaat ataagaaagg cacgagaagc gtcagcaaaa tgccacgtta tattatcgaa
     5161 tatttgggcg tgaagaacgg agaaaatgtt tatcgggtta ctgccaaggc ttggggtaag
     5221 aatgccaata ccgtggtcgt ccttcaatct tatgtaagca ataatgatga gtaataaaat
     5281 ggaacaaaaa gggtttacat tgattgaggt aatgatagtt gtcacgatac tcggcattat
     5341 cagcgtcatt gccatacctt cttatcaaag ttatattgaa aaaggctatc agtcccagct
     5401 ttatacggag atggtcggta tcaacaatat ttccaaacag tttattttga aaaatcccct
     5461 ggacgataat cagaccatca agagcaaact ggaaatattt gtctcagact ataagatgaa
     5521 tccgaaaatt gccgaaaaat ataatgtttc ggtgcatttt gtcaataagg aaaaaccaag
     5581 ggcatacagc ttggtcggcg ttccaaagac ggggacgggt tatactttgt cggtatggat
     5641 gaacagcgtg ggcgacggat acaaatgccg tgatgccgct tctgcccgag cccatttgga
     5701 gaccttgtcc gcagatgccg gctgtgaagc cttctctaat cgtaaaaaat agggctgttt
     5761 tgccgatgcc gtctgaaaat caatgttcag acggcatttt tat
//
LOCUS       DAWFKN010000109         5738 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFKN010000109 DAWFKN010000000
VERSION     DAWFKN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.109"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            129..350
                     /locus_tag="WGR46_001970"
                     /pseudo
     CDS             129..350
                     /locus_tag="WGR46_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(332..640)
                     /locus_tag="WGR46_001971"
     CDS             complement(332..640)
                     /locus_tag="WGR46_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856834.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
     gene            complement(699..931)
                     /locus_tag="WGR46_001972"
                     /pseudo
     CDS             complement(699..931)
                     /locus_tag="WGR46_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            867..1094
                     /locus_tag="WGR46_001973"
     CDS             867..1094
                     /locus_tag="WGR46_001973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856835.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            1256..2311
                     /gene="aroG"
                     /locus_tag="WGR46_001974"
     CDS             1256..2311
                     /gene="aroG"
                     /locus_tag="WGR46_001974"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0856836.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            2379..2867
                     /locus_tag="WGR46_001975"
     CDS             2379..2867
                     /locus_tag="WGR46_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0856837.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(3217..3693)
                     /locus_tag="WGR46_001976"
     CDS             complement(3217..3693)
                     /locus_tag="WGR46_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0856838.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKIMETMCPFKMEKRRYPVRWNGSLFEVD
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(3705..4133)
                     /locus_tag="WGR46_001977"
     CDS             complement(3705..4133)
                     /locus_tag="WGR46_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0856839.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQNYSNTFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            4245..4490
                     /locus_tag="WGR46_001978"
     CDS             4245..4490
                     /locus_tag="WGR46_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856840.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVRKLYSEQKMQKFVWEKRDLIADYISRRMQG"
BASE COUNT         1511 a         1367 c         1420 g         1440 t
ORIGIN      
        1 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
       61 agcaccaagt gaatcggttc cgtactattt gtactatctg cggcttcgtc gccttgtcct
      121 gatttttgtt aatccgctat attttagata atgcgcgatt tcaccgtatg ggtgtcttac
      181 gggaaatggc ggaaaaattg ggacataagg tattgcctct tgcaccttat tcacctgagc
      241 tcaacccgat tgagaaagtg tgggcgaata ttaagcggta tctgcgaacc gttttgtctg
      301 attacgcccg atttgacgat gcactactgt cctactttga ttttaattga ctatagaacg
      361 ttgcggttac gcggaagccg tactcgttgg atttggagcg gcccattttg gttttgtcgc
      421 cgtccaaaac aatctcacgg ggtttgcaga ttgttttgtg acggtagtat ggatcaaact
      481 cgagaccgac gctgtcggtc aactgtttgc ctacattcag accgataccg acactccaac
      541 ctttggcgct tttgctgaca tcgcgggaag cacacatctg ggtcgtcatc actttggttt
      601 tgccgcgcaa atctgcatat gcatccgccc aaggggtcag ggatcatccg tcccccaaat
      661 cttggcggat ttcgccatag actttcaaaa caaggttttc atgcttggta acggtgtttt
      721 tccttatcgc cgatgatggc tttgcccttg ccgttagact caggaatatc ggctaccgta
      781 acggcggaca cggctgcaag tgagagtgca agcagggttt tttcatgttt ttcttcctat
      841 aatgaggatg aataaatgga gaaagtgtgg gaaatacccg cattccaatt aaatcttttt
      901 tcaagcaatt agttcttttt gttttcaaca ttttccttgc gtatgaaatt ttgccggatg
      961 tgcaggcgga atcggcagcg gggtgttctg taccggattg tcgtggaaat gggaaaacgg
     1021 atgttccgtg cggttttgtc cgaatgaatg gcgggtattg tttttatcaa tctgtttctt
     1081 tttctttgga ataaaattcc taaaatatta aaaatatgaa atttaaaatc tatgaaaaaa
     1141 gatatatcag ttattttgaa ataaaatagc tttgtagtaa tatgttgcac ttgtttgtgc
     1201 aaggtaaacg atgtaaccta agccgcgtat aaaaaaccat caggaaagat gcaagatgac
     1261 acaccattac cccacagacg atattaagat taaagaagtt aaagagttgt tgccgccgat
     1321 tgcccatctt tacgagctgc cgatttccaa agaggcttcg ggcttggttc accgcacccg
     1381 tcaggaaatt tccgatttgg ttcacggcag ggacaagcgg ctgttggtta ttatcgggcc
     1441 gtgttcgatt cacgatccga aagcggcgtt ggaatatgcg gagcgtttgt tgaaactccg
     1501 caagcagtat gaaaacgagc ttttgattgt gatgcgcgtt tatttcgaga agccgaggac
     1561 gacggtgggt tggaaaggtt tgattaacga cccgcatttg gacggtacgt ttgacatcaa
     1621 tttcggtttg cgtcaggcgc gcagcctgtt gttgtcgctg aacaatatgg gtatgcctgc
     1681 ctctaccgag tttttggata tgattacgcc gcaatattat gcggacttga tttcttgggg
     1741 ggcaatcggt gcgcggacga ccgaaagcca agttcaccgc gaattggcaa gcgggctgtc
     1801 ctgccccgtc ggctttaaaa acggtacgga cggcaatttg aagattgcca tcgacgcaat
     1861 cggtgcggcg agccattcgc atcatttttt gtctgtaacc aaggccgggc attccgccat
     1921 tgtccatacc ggcggcaatc ccgactgtca tgtcattttg cgcggcggca aagagccgaa
     1981 ttatgatgcg ggacacgtca gcgaggcggc ggaacaactg cgtgcggcag gcgtaaccga
     2041 caagctgatg atagattgca gccacgccaa cagccgcaag gattacactc ggcagatgga
     2101 agtggcacaa gatattgccg cccaattgga acaggacggc ggcaatatca tgggcgtgat
     2161 ggtggaaagc catttggtcg aaggcaggca ggacaagccg gaagtgtacg gaaaaagcat
     2221 taccgatgcg tgtatcggtt gggacgcgac tgaagaactg ttggcattgt tggcaggtgc
     2281 aaacaaaaaa cgtatggcgc gcgccggttg agatttttga cacggaatgt cataaaatgc
     2341 cgtctgaagc gttcagacgg catttttgtg gaggaaatat gctcaaaata accctaattg
     2401 cggcgtgtgc ggaaaacctg tgcatcgggg cgggcaatgc tatgccttgg cacatccccg
     2461 aagatttcgc atttttcaaa gcctatacct tgggcaaacc cgtcattatg gggcggaaaa
     2521 cgtgggaatc cctgcccgtc aaacccctgc ccggacggag gaacatcgtc atcagccggc
     2581 aggcggatta ttgcgcggca ggcgcggaaa cggcggcaag tttggaggcg gcattggcat
     2641 tgtgcgcagg cgcggaagaa gccgtcatta tgggcggcgc gcagatatac ggacaagcga
     2701 tgccattggc gaccgatttg cggataaccg aagtggattt gtctgtggaa ggagatgcat
     2761 ttttccccga aatagaccgg acgcattgga aagaggcagg gcggacggaa cgccgcgtca
     2821 gcagcaaagg cacgagctat gcttttgtgc attatttgag atattgaaat ataaactctc
     2881 tataaaatcc cccgcaaatg atgggctgaa atagaaaata ttgttattcc cccgaagatg
     2941 ggaatccggg attttaaagt tagggtaatt tatccgaaat aacaacaatc ttccatcgtc
     3001 attcccgcaa aagcgggaat ccggaaacga aaagctaaag caatttatcg gaaaaaaccg
     3061 aagtttaaag aaccggattc ccgcctgcgc gggaatgacg agattttagg ttatggggat
     3121 ttattgggaa taatggaaca aagaaagcag aaataaggat atagaggctg tctttggatt
     3181 tgcgatggtt gtcggagaat gccgtctgaa gccgcttcag acggcatttt tccagcttga
     3241 gaacggatgt ttgctcaaat aagcattggt aaacataccg tcggcagtga tttcgcgccc
     3301 cagccagtcc ggacggtcaa aatcggcatt ttcgtcgggc agctcgattt ctgcgacgac
     3361 caaaggtgcg ttttctccaa ggaaaacatc gacttcaaac aggctgccgt tccatctgac
     3421 cggataacgc cgtttttcca tcttaaacgg gcacatcgtt tccattatct tttccgcatc
     3481 ggcaagcggg atttcgtatt caaactcact gcggctgatt tccgaaatat agcctttcag
     3541 cgtcagccac gcccgttttc cggcaatgcg gacacggacg gtgcgttctt tttcaacaga
     3601 cagataaccc tgcctcaaca gcagcggctc atcggcgtat tgccgccagt tgtcgtttcc
     3661 aatcaaaaaa cggcgttcga tttctatcgg cataagatgc tccgtcaaaa cggtttgaac
     3721 acgaccagat acagcgcggc aaccatcagc agcacgggga tttcgttaaa tacgcggtac
     3781 cagcggtgtg agaaagtatt gctgtaattc tgaaaacggc gcagcagcac gccgcaatac
     3841 aactggtaag ccaaaagcat caagcccaaa cacagtttga cgtgtaccca gccgctgccc
     3901 caccagccgg cggcaaacgg tatcgccgcg ccgaacacga ccgcaccgaa acctaaaggg
     3961 gacataaaac ggtacaaccg caccgccata cccgacaggc gcacatactc gggattgccg
     4021 cgcggcacat caatcatcgc catattgacg aaaatcctcg gcaggtaaaa cagccctgca
     4081 aaccacgaaa tgacaaaaaa caagtgaaac agcttgaacc aagaaaacat catcgcccac
     4141 accctgccga aaagcggtat tgtacaggca aaccgcttgg gaaacgtgat aaaatcaggc
     4201 ggataaacaa atcgaataaa tccttaccgc aaaacggagg caaaatgctc aaatccatcg
     4261 aactcaattc ccacatccgc aaccgccttg cagcatatct gaaaggcagg ggtatggatt
     4321 ttcagacggc gatgcgggag gaaaaaggca acaaagaaat cgcctccatc gtccacagcg
     4381 gtctgcccac tctggtccgc aaactgtatt ccgaacaaaa aatgcagaag tttgtttggg
     4441 aaaagcggga tttgattgcc gactacatca gccgccggat gcagggatag gcggctgaaa
     4501 tctgttttca ggcaagtgaa aagacaatat ggcagattga aattatgctt atcgtcattc
     4561 ccgcccgcgc gggaatccga cttgtttggt ttcggttatt tttcgtttcg taacttttga
     4621 gccgtcattc ccgcgcaggc ggtaatccgg cttgttcggt ttcggttctt tttctcgttt
     4681 cgggtgattt ctaaaccgtc attcccgcgc aggcgggaat ctaggtcttt aaacttcggt
     4741 tttttccgat aaatttttgc cgcattaaaa ttctagattc ccgctttcgc gggaatgacg
     4801 gcggagggtt tttagttttc ccgaaaatgc acatcatcca aaatcccgtt attctcacaa
     4861 aacagaaaat caaaaacaac aatctgaaat tccgtccttc ccgcccgcgc gggaatccga
     4921 cttgtccggt ttcggttctt tttctcgttt cgggtgattt ctaaaccgtc attcccgtgc
     4981 aggcgggaat ccagaccttt aaaccccgga catccccgat aaattcttgc ggcattgaaa
     5041 ttctagattc ccgctttcac gggaatgacg gcggagggtt tttagttttc ccgataaatg
     5101 cacatcatcc aaaatcccgt tattcccaca aaacagaaaa tcaaaaacaa caatctgaaa
     5161 ttccgtcctt cccgcccgtg cgggaatccg acttgtccgg tttcggttct ttttctcgtt
     5221 tcaggtgatt tctaaaccgt cattcccgcg caggcgggaa tccagacctt caaacctcgg
     5281 tcatccccga taaattcttg cagcattaaa attctagatt cccgctttcg cgggaatgac
     5341 ggcggagggt tttttgtttt cccgataaat gcacatcatc caaagtcccg ttattcccac
     5401 aaaaacagaa aatcaaaaac agcaacctga aatcccgtcc ttcccgcgca ggcggtaatc
     5461 tgaacacgtc cgtagtgaaa cctatatccc gtcattcgca cgaaagtggg aatccaggat
     5521 gcagggaaaa ccgttttatc cgataagttt ccgcaccgaa aggtctagat tcccgctttc
     5581 gcgggaatga cggcggaggg tttttagttt tctcgataaa tgcacatcat ccaaaatccc
     5641 gttatttcca caaaacagaa aatcaaaaac agtaacctga aatcccgtca ttcccgcgca
     5701 ggcgggaatc cggcttgttc ggtttcggtt ctttttct
//
LOCUS       DAWFKN010000110         5662 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFKN010000110 DAWFKN010000000
VERSION     DAWFKN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.110"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            100..816
                     /locus_tag="WGR46_001979"
     CDS             100..816
                     /locus_tag="WGR46_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0856841.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            965..1867
                     /locus_tag="WGR46_001980"
     CDS             965..1867
                     /locus_tag="WGR46_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0856842.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1910..2683
                     /locus_tag="WGR46_001981"
     CDS             1910..2683
                     /locus_tag="WGR46_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0856843.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2795..4483
                     /locus_tag="WGR46_001982"
     CDS             2795..4483
                     /locus_tag="WGR46_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0856844.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4576..5532
                     /gene="gshB"
                     /locus_tag="WGR46_001983"
     CDS             4576..5532
                     /gene="gshB"
                     /locus_tag="WGR46_001983"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0856845.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1309 a         1541 c         1568 g         1244 t
ORIGIN      
        1 taaatttgtg caatgcttta attcctctga cacgatttga agcacagccg ccttaaaaca
       61 tcttaaaaaa tttgaaagcg taaaaatacg gaaaacatca tgaactttga aaacgacgac
      121 attatccatg cgccgaccac gtcttccctg attctcgaag agcggcacga ttcggagctg
      181 ttccgtgttt acgccctgat tttggacggc attaccgatc aggtgctgct gcctggcaaa
      241 aagctgaccg aatccgaact ttgccgccag atggtgtgtt cgcgcaacac cgtccgcggc
      301 gcgctgtcgc ttttggcgca cgacaagatt gtcgatttgc aacccaacag gggcgcgttc
      361 gtccacgttc ccgatttgaa agaaatgcag gatgtgttca atgcgcgcat cgaaatggag
      421 acgatgattt tgaatatcct cgcagatttg ccggatttgg aaacgcgcct caagccgctt
      481 tatgcgatga tacggcgcga agaagaggcc tccggcaggg gagaccgcgt tggctggaac
      541 cgcctgtcca atgcctttca cgtcgaactg gcgcgcctgg tgggcaacga tgtgctgttc
      601 gacattatga acacgctgtg cgcgcggtct tccctgattg tcgccgtggc gggcgtgcat
      661 cgcgaggaaa aacacgccat caatacgcat acgcattccg aacaccgcga aatcctcgac
      721 ctgctgctgg cgggcaggcg caaccgcgtg gtcaaaatcc tgcgccgcca tttgggcaac
      781 tgtatggagc gtttggaaaa gactttggaa gattgaatgc cggagcggat aagccgcctg
      841 aaccttcagg cggtttttta atggcgaacc tgatgccgtc tgaaatatgg atgcgggtat
      901 ctgcaatttt cagacggcaa tttttaaacc gcatatatca tgcggcaata aaggaggtca
      961 ggagatggac agcctgatga cgttgctttc ggtattgata ccgatgtttg ccggattttt
     1021 tatccgtgtg cccaagcctt acctgcccgc tttggataag gtgctatcgg tcttggtgta
     1081 tgctgtgctg ctgctgatcg gcgtctcgtt gtcgcgcgtg gaggatttgg gttcgcggtt
     1141 ggacgatatg gcgttgacgg ttctgtggct gtttgtttgt acggtcgggg cgaacctgct
     1201 tgctttggca gtgttgggaa agttattccc gtggcggata aaggggaaag ggaagggcgt
     1261 ttcggtcggt gtgtcgggca gtgtggggca gctcggatgc gtgctgctcg gatttgcatc
     1321 cggcaaactg atgcgcgata tttggatgcc gtctgaaaac gcgggtatgt attgtctgat
     1381 gctgctggtg ctcctcatcg gcgtacagct caaaagcagc ggcgtatcgt tgcggcaggt
     1441 tttggtcaac cgcaggggta ttcggttgtc ggtctggttt atgctttcat ctctttcagg
     1501 cgggctgctg tttgccgcat cggcagacgg tgtgtcgtgg gtgaaaggtt tggcgatggc
     1561 ttccggcttc ggttggtatt ccctctcggg tttggtgatg accgaggctt acggcgcggt
     1621 atggggcagt atcgcgcttt tgaacgattt ggcacgagag ctgttcgcgc tggcatttat
     1681 tccgctgctg atgaagcgtt ttcccgatgc ggcagtgggg gtcggcggcg cgaccagtat
     1741 ggatttcaca ttgcccgtga ttcggggtgc gggcggcttg gaagccgtac cggtagcggt
     1801 cagcttcggc gtggtggtca atatcgccgc tccgtttctg atggtggtgt tttccgcttt
     1861 gggctgaacg cggtaaaatc ggcatcccga tgcaaggagg cagaaaacga tgaaaccgaa
     1921 aatccaaagg cacggagaga ttttaagcct tgtccgccgg catcagttta tgtcggtgga
     1981 cgagcttgcc gccgcattgg acgttacccc gcagacgata cgccgcgaca tccgcgaatt
     2041 ggaggatgct ggcagcctga aacgccacca cggcggcgca tcctcgggcg gaaacttgcc
     2101 ggaggggctg cctgccagcc gccaaattca atgtcaaaac gaaaaaaacg ccattgcccg
     2161 gctgattgcg gaacatattc ccgacgagtc atcgctgttt gtcagtatcg gcacgacgat
     2221 ggaagccgtg gcatcggagc tggtgagacg gcgcggcagc ctgcggatta ttaccaacaa
     2281 tatccacgtc gcctccatcg tttcggcgcg gacggattac acggtcatca tcacttccgg
     2341 agtcgtccgc cctttggacg gcggcattac cggcgtggca accgtcgatt ttatcaacca
     2401 gttcaaagtc gattatgccg tgatgagtac gcacggcgtg gaaagcgacg gttcgctttt
     2461 ggacgacgac tacaaagaag tcagcgtgat gcaggcgatg attgccaacg cgcgcgtccg
     2521 cttcctcggc gtggaccaca gtaaattccg cagcaacgcg ctggtcaggc tcggcgacat
     2581 tacggcgttt gacaaagtat ttaccgaccg gctgcccgat accgcgatgc agaagatgct
     2641 gaaagaggcg ggggtggaat gcctgattgc cgatgccgtc tgaacgctat gtcaaagcgc
     2701 gcaagtcggg tacaataaac acatcatcaa accgcttcag acggcataag gaaccgcccc
     2761 aatgccgtct gaagccatct gttcaaagaa aactatgctc aataaagacc aatttgcgga
     2821 caaccatttt atccgcacca tcatcgaaga cgacctcaaa agcggcaaac acgaagccgt
     2881 ccaaacccgt tttccgccgg agcccaacgg ctacctgcac atcggacacg ccaaatccat
     2941 ctgcctgaac ttcggtttgg cgtatattta cgacggcttg tgcaacctgc gttttgacga
     3001 taccaacccc gaaaaagaaa acgacgaata cgtcaacgcc atcaaagaag atgtcgagtg
     3061 gctcggtttc cattgggcgg gcgagccgcg tttcgcttcc aactatttcg accagcttta
     3121 tgactacgcc gtcggtttga ttaaagacgg caaagcgtat gtcgatgatt tgacgcccga
     3181 agaaatgcgc gaataccgcg gtacgctgac cgaagcgggt aaaaacagcc cttaccgcga
     3241 ccgcagcgtc gaagaaaacc tcgacctgtt cacacgcatg aaaaacggcg agttccccga
     3301 cggcagcaaa accctgcgcc tgaaaatcga catggcatcg ggcaacatca atatgcgcga
     3361 ccccgtcatc taccgcatcc gccgcgccca tcaccacaac accggcgaca aatggtgtat
     3421 ctacccgatg tacgactaca cgcattgcat ctccgatgcc atcgaaggca tcacgcattc
     3481 cttgtgtacg ctcgaatttg aagcacaccg tccgctgtac gactgggtgt tggataacat
     3541 ccacgcgccg catgccaccc gtccgcgcca atacgagttt tcccgtttgg agcttttgta
     3601 caccattacc tccaaacgga aattgaatca gttggttgtg gaaaaacacg tttccggctg
     3661 ggacgatccg cgtatgccga ctatttccgg tatgcgccga cgcggctaca cgcccgaagg
     3721 cctgcgtctg tttgccaaac gcgccggtat ttccaaatct gaaaacatcg tcgatatgag
     3781 cgtgttggaa ggcgcgattc gcgaagagtt ggaaaattcc gccccacgcc tgatggcggt
     3841 gttgaacccg ctcaaagtga ccctgaccaa ctttgaagcc ggcagaaccc aaagccgccg
     3901 tgccgcgttc catccgaacc acgaggaaat gggcgagcgc gaagtaccta tttcacaaac
     3961 catctatatt gaagccgacg actttgccga aaatccgccc aaaggcttca aacgcctgat
     4021 tcccggcggc gaagtacgct tgcgccacgg ctatgtcatc aagtgcgatg aagtcgtcaa
     4081 agacgaagca ggcaatgtgg ttgaactcaa atgcagcatc gaccacgaca ccttgggcaa
     4141 aaatccagaa ggccgtaaag ttaaaggcgt gattcattgg gtttccgccg aacacgccgc
     4201 cgaaatcaaa gtccgtctgt acgaccgcct ctttaccatc gagcgtcccg atgccgtgcg
     4261 cggcgaagac ggcgaatacc tgccgttcac cgatttcctc aatccggaat ccgtcaagga
     4321 aatcaccgcc tacgccgaac ctgccgccaa agatttgccg gcagaaagcc gttggcagtt
     4381 cgagcgtatc ggctacttcg taaccgaccg taaagaccac ggcaaagaca cgccggtgtt
     4441 taaccgcacg gtcacgctga aagattcttg gcagcctaag taaaacccca tccttgccgt
     4501 ctgaatattg ttcggacggc atttcccctt tgcccgcgaa atgcggcaca ttcggcacac
     4561 cggaaaggaa atatgatgaa agtcctcttt atcgccgacc cgatggcaag tttcaaaacc
     4621 tacaaagaca ccacctacgc catgatgcgc gagatggcaa aacgcggttg gcggctgttt
     4681 cataccttga gcggggaatt gtcggttaaa aacggtctgg tggttgctga tgcgtctgct
     4741 tttgagtttc tcggcgcaca agacgaccac gacaaaaact ggtttgccgc cgccgacaaa
     4801 gttcagacgg cattgaaagc atttgatgcc gtgattatgc gtaccgatcc gccgttcgat
     4861 atgcaatacc tctacgccac ccaattactg acgctggcgg aacagcaggg cgcgaaagtc
     4921 tttaacagcg gacagtcgat gcgtgacttc aatgaaaaac tggcgatttt gaacttcagc
     4981 cgctttaccg cgcccacgct ggtaacgacc cgttccgccg atgtccgcgc atttttgaaa
     5041 gaacacggcg acattatcat caaaccgctg gacggtatgg gcggtatggg tattttccgc
     5101 ctgaccgaaa aagaccccaa catcggcagc atcctcgaaa ccctgatgcg gcttgattcc
     5161 cgcaccatta tggcgcaacg ctacattccc gaaatcgtac acggtgacaa acgcatcctg
     5221 attatcggcg gcgaagtcgt cccctatgct ttggcgcgta tcccgcaaaa cggcgaaaca
     5281 cgcggcaacc ttgcggcagg cggacgcggc gtggcgcagg aattggacgg acgcgaccgc
     5341 gaaattgccg aaacgctcgc ccccgaactc aaacggcgcg gcatcctgct ggccggtttg
     5401 gacgttatcg gcagcaacct gaccgaagtc aacgtaacca gcccgaccgg attccaagaa
     5461 attatgaaac aaaaaggttt cgacgtggcg gcaatgtttg ccgatgccgt tgccgcgtgg
     5521 tcggtacgtt aaaccgatgc cgtttgaaag gcttttgctt cacaaccgtt tggtttgttc
     5581 gggcaggcag ggttttccct cagccggcag ggatgcggtt aatgccgtac ggacaccccg
     5641 ccgccccgtt ttcagacggt at
//
LOCUS       DAWFKN010000111         5647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFKN010000111 DAWFKN010000000
VERSION     DAWFKN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.111"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(713..1138)
                     /locus_tag="WGR46_001984"
                     /pseudo
     CDS             complement(713..1138)
                     /locus_tag="WGR46_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            1286..1738
                     /gene="dut"
                     /locus_tag="WGR46_001985"
     CDS             1286..1738
                     /gene="dut"
                     /locus_tag="WGR46_001985"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0856846.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1814..3001
                     /gene="dapC"
                     /locus_tag="WGR46_001986"
     CDS             1814..3001
                     /gene="dapC"
                     /locus_tag="WGR46_001986"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0856847.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            3159..3938
                     /gene="yaaA"
                     /locus_tag="WGR46_001987"
     CDS             3159..3938
                     /gene="yaaA"
                     /locus_tag="WGR46_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0856848.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRKLAPQ
                     QIAELMHVSDKIALLNAERNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLMQPYRLEMGTPFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            4021..4111
                     /locus_tag="WGR46_001988"
     tRNA            4021..4111
                     /locus_tag="WGR46_001988"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4055..4057,aa:Ser,seq:tga)
     gene            4189..4278
                     /locus_tag="WGR46_001989"
     tRNA            4189..4278
                     /locus_tag="WGR46_001989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4223..4225,aa:Ser,seq:cga)
     gene            complement(4474..5586)
                     /locus_tag="WGR46_001990"
                     /pseudo
     CDS             complement(4474..5586)
                     /locus_tag="WGR46_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1386 a         1341 c         1420 g         1500 t
ORIGIN      
        1 gcgggaatga cgaatccatt cgtacggaaa cctgcaccac gtcattccca cgacagtggg
       61 aatccagttt tttgagtttc agtcattccc gataaagtgc cttagcattg aatgtctaga
      121 ttcccgcctg cgcgggaatg acggatttta gtttgggggg catttattgg aaaaagcaga
      181 aaccgctccg ccgtcattcc cacgaaagtg ggaatccagg atgcagggtt ggggaaaccg
      241 tgttatccga taagtttccg caccgacagg tctagattcc cgcctgcgcg ggaatgacga
      301 acctatccgt acggaaacct gcaccgcgtc attcccacga aagtgggaat ctagtttttt
      361 gagtttcagt cattcccgat aaattgcctt agcattgaat gtctagattc ccgcctgtgc
      421 gggaatgacg aatccattcg tacggaaacc tgcaccacgt cattcccacg aaagtgggaa
      481 tctagttttt tgagtttcag tcattttcaa taaattgcct tagcattgaa tgtctagatt
      541 cccgcctgcg cgggaatgac gattcatata gtggattaac aaaaatcagg acaaggcggc
      601 gaagctgcag acagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga
      661 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
      721 aaaaaggcat attgaatgcg ggcaaaccgg ctgctttccg tttttggatt tcggagaatg
      781 ccatcgccca gctttcatca cacataaaaa acagtgcggg cacggctttt ttcagcggta
      841 tttctttcag gtgcagggca agtgccgccc ccatcaggat atgccgcgaa ttaatcataa
      901 aggttacggt ggcgataagc agtatcggca gagattccgc ccacaggttg accgtggcaa
      961 actcggagcc gccggcgaag ttcatactgg tcatcaacaa catttccagc cagctcatgc
     1021 ctttttgtcc gccctgcata ccgagtatta atgcccaagg cagaagtccg ataagtatgg
     1081 gcgaactttc tttgatgccg cgtataaatt cgttacgggg cgagcttatg tgtgtcatat
     1141 tataatattg atatgtaaga aggtcgggca ttatacacag acggagtatt tcaaaacaaa
     1201 acctatttgc tgtgtttcag tgtaaacacg gcttgtgtat aatccaccat ctttagacag
     1261 accgaccgta tgtgggagca aggcaatgaa tattgaagta gaaatgaaag tgttggacga
     1321 gaggatggcg gattttatcc ctacctatgc gacagcgggt tctgccggtt tggacttgcg
     1381 cgcctgtttg gatgaggaag tcgtcttgca gccgggggaa acgtttcttg tgccgacagg
     1441 tttggcaatt tatttggcca atccttcata tgccgccgtt ttgctgcccc gttccggctt
     1501 ggggcataaa cacggcattg ttttgggcaa tttggtcggc ttgattgact ccgattatca
     1561 aggggaattg aaggtttcgt tatggaacag aagcagcgag ccgtttactg tcaaaccgtt
     1621 tgagcgtatc gcgcagatgg ttatcgtgcc aatcgtgcag gcgcgtttca aacgtgtcga
     1681 ggaatttgtc ggaagcagcc gtggagaagg cggtttcggc agtacgggtt tgcattgaat
     1741 ataaaatgcc gtctgaaggg cacgtcaggt tcagacggca tatcttccga tataccgata
     1801 accggagaaa atcatgaata ccttactcaa gcagctcaaa ccctatccct ttgcccgact
     1861 gcacgaggcg atgcagggca tttccgcgcc tgaaggcatg gaagccgtcc ccctgcacat
     1921 cggcgaaccg aaacatccga caccgaaagt cattacggat gcgctgaccg cctcattgca
     1981 cgagttggaa aaatatccgc tgactgcggg tctgcctgaa ctgcgtcagg cgtgtgcaaa
     2041 ctggttaaaa cgccgttacg atggcttgac agtggatgcg gataatgaaa ttctgccggt
     2101 tttaggcagt agggaggcgt tgttttcttt tgttcaaacc gtgttgaacc ctgtttcaga
     2161 cggcatcaaa cccgcaattg tcagcccgaa tcccttttat cagatttacg aaggtgcgac
     2221 acttttgggc ggcggtgaaa tccattttgc caattgcccc gcgccgtctt tcaaccccga
     2281 ttggcgtagt atttccgaag aggtttggaa acgcaccaaa ctggtgttcg tctgctcgcc
     2341 caacaacccc agcggcagcg tgctggattt ggacggatgg aaagaagttt ttgatttgca
     2401 ggacaaatat ggtttcatta ttgcctcgga cgaatgctat tccgaaatct atttcgacgg
     2461 caacaaacct ttaggctgcc tgcaggccgc tgcacagttg gggcgaagca ggcaaaaact
     2521 gcttatgttc accagtttgt ccaagcgttc caacgttccg ggcctgcgtt ccggttttgt
     2581 cgccggcgat gccgaactgc ttaaaaactt tctgctttac agaacctatc acggcagtgc
     2641 aatgagcatt cccgtgcagc gcgcaagcat tgccgcttgg gatgacgaac agcacgttat
     2701 cgacaaccgc cgcctgtatc aggaaaaatt tgagcgcgtt attcccattt tgcaacaggt
     2761 atttgacgtt aaattaccgg atgcctcgtt ttacatctgg ttgaaagtcc ccgatggcga
     2821 cgatttggca tttgcacgca atttatggca aaaagccgct atccaagtat tgcccggacg
     2881 ttttttggcg cgggataccg aacagggcaa tcccggggaa ggttatgtcc gtatcgcttt
     2941 ggttgccgat gtcgcaactt gtgtcaaagc tgcggaagac atagtttcac tgtatcggta
     3001 aggaatcggc aatgcagtct gaacttttgt tcagacggca tttttcaata ttttacggtt
     3061 gaatttgctg taacggtatt tacttcactt ttaatcggct tgcccgcaaa cacgtttaaa
     3121 cttaaaatcc ccatgtttga cacaataccg agcggattat gttttttgtc ctttcccctg
     3181 cgaaaaacct taacgaaaaa gaccctgccc ctgtcagtga gtttacccaa cccgacctgc
     3241 tggcagagtc cgaaattcta atgcagcagt tgcgcaagct tgcgccgcaa cagatcgccg
     3301 aactgatgca cgtttccgac aaaatcgcac tcttaaacgc cgaacgaaat gcagaatggc
     3361 acacgccgtt tacgccggaa aacgccaaac aggcggtctt tatgttcaac ggcgatgttt
     3421 acgaaggtat ggatgcaaac acattggata ttggacagat acgctatctg caaaaccatg
     3481 tccgcctgct gtccggtctg tacggtcttc tgcgtccact ggatctgatg cagccctacc
     3541 gcctggaaat gggaacgccc ttcgccaatt tgcgcggcaa gaatttgtat gagttttggg
     3601 gcgacatcat taccaacctt ttaaatgaca cgcttgccca agcaggcagc aatacgcttg
     3661 tcaatcttgc ctcacaggaa tattttaagt ccgtcaacac gaaaacactt cgggcgcggc
     3721 tgattacccc gatatttaaa gacgaaaaaa acggcaaata taaaattatc agtttctatg
     3781 ccaagcgtgc acgcggatta atggtgcgct atgcggcaga acatcatatt accgaccctg
     3841 aaatgctgaa aaattttaat tacgaaggct acgcattcaa tgacgcggct tcaaatgaaa
     3901 gcgaatgggt ttttatgcgt tcggaacaaa taaagtgaaa acaataaatt aagtattttc
     3961 caaaaaaagt gcttggcaaa atgtataaac ttcattatta ttcctaatct tcaagaagac
     4021 ggaagcgtgg cagagcggtt taatgcaacg gtcttgaaaa ccgtcgaggg ttgatagccc
     4081 tccgtgagtt cgaatctcac cgcttccgcc aatccttgag gtcaaaaacc aaataaaaat
     4141 gaagtagcat aaagcatcgg catttttatt tggttttact tcattatcgg aaacgtggca
     4201 gagaggctga atgcagcgga ctcgaaatcc gctgagggtg caaatcctcc gtgggttcga
     4261 atcccaccgt ttccgccaca aaacaaaacc gccctgattc ggggcggttc ttttttgttc
     4321 aagttgagac ctttgcaaaa ttcccttccc tcccgacagc cgaaacccaa acacaggttt
     4381 tcggctgttt tcgccccaaa taccgcctaa ttttacccaa atatcccctt aatcctgccc
     4441 ggatacccga taatcaggca tccgggctgc cttttaggcg gcagcgggcg cacttaacct
     4501 gttggccgct ttcaacaggt tcaaacacat cgccttcagg tggctttgcg cactcacttt
     4561 aatcagtccg aaataggctg cccgagcgta gcggaattta cggtgcagcg taccgaagct
     4621 ctgttcgacc acatatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg
     4681 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata
     4741 acgggtcttc gacaaatatc ggttgcgttt ggtttgcgcc tccgtcagcg gacggttgcg
     4801 gcaggctttg cgcataatgc cgtccggtaa ctgatgttct ttcagatgtt gccggttttc
     4861 cgcactgtca tagcctttgt cggcatagac ggtcgtacct tcagctatcc cttccaacaa
     4921 cggcgacagg tgtgtgcact catgggcatt ggcgggagta atgtgcagtt tctcgatata
     4981 gccttcctca tcggtacggg tatgttgttt gtaaccgagt ttgtagaggc cgtttttctt
     5041 tatccaacgg gcatcgctgt ccttactcgg tgtggtttgg ccgctgattt gtccttcttc
     5101 gtcaacttct atggcctgac gctgtttgct gccggcggtc tgaataatgg tggcgtcaac
     5161 aacggcggcg gatgctttct ctatttttag gcctttttcg gtcagttgtc ggttaatcag
     5221 tttgagcaat tcggacaggg tattgtcttg agccagccaa ttgcggtagc ggcataaggt
     5281 gctgtaatcg gggatgctta gttcgtcaaa acggcaaaac aggttgaaat cgatgcgggt
     5341 gatgaggctg tgttcgagtt cgggatcgga gagactgtgc cattgtccga gcaggacggc
     5401 tttgaacatg gacaacaggg gataggcggg acggccgcgg tggtctcaga ggtaacgggt
     5461 cctttgacgg ttcaggtatt gctcgatcag ttgccaatca atcatctgat ccaacttcaa
     5521 tagcgggaaa cggtcgatgt gtttggcaat catggcttgc gcggtttgtt gaaagaaggt
     5581 gctcatggaa catctcctaa atgtcttggt gggaatttag gggattttgg ggaattttgc
     5641 aaaggtc
//
LOCUS       DAWFKN010000112         5565 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFKN010000112 DAWFKN010000000
VERSION     DAWFKN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.112"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGR46_001991"
     CDS             complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGR46_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0856849.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTEQICRLGNNT
                     YTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLGKI
                     DWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3207..5372)
                     /locus_tag="WGR46_001992"
     CDS             complement(3207..5372)
                     /locus_tag="WGR46_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0856850.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPTAMPKENEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISNVQTDNGDNNIYISPYLTQSSHNSHNGSINGGANQPKNEATGYNNFKYV
                     YSGWFYKHAASERKFNEGKFKSGDDGYIFYHGKDPSRQLPASGKVTYKGVWHFVTDTK
                     LGQKFNDIIQPSKKQGDSYSGFSGDEGEEYSNKNESTLKDGHEGYGFTSNLEVDFGNK
                     KLTGKLIRNNASQSNTNNDKPTTQYYSLEAQVTGNRFNGKAIATDKPQENETKQHPFV
                     SDSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKPRNGAVASGGTGAAASDG
                     AAGTSSKNGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QANQGTNGGTAFTYKTTYTLESDKKDTKAQTGAGGAQAASGAAGVNGGQAGTKTYEVE
                     VCCSNLNYLKYGMLTRKNSKSAMQAGENGSQADAKTEQVEQSMFLQGERTDEKEIPKE
                     QQDIVYRGSWYGHIANNTSTSWSGNASNATSGNRADFTVNFGDKKITGTLTANDRTQP
                     TFTITANIKDNGFEGTAKTAELGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGG
                     WFAYSGDKQTENTTVASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         1158 a         1415 c         1383 g         1609 t
ORIGIN      
        1 cccatcattc ccgcgcagga gggaatccga atacatccga acagaaacct gcatcccgtc
       61 attcccgcga aagcgggaat ctagaacgta aaatctaaag aaaccgtttt atccgataag
      121 tttccgcacc gacaggtcta gattcccact ttcgcgggaa tgacgggatt tgaggtttct
      181 gtttttgatt ttctgttttc gtaggaatga cggtttagag ttacccgaaa cctcaaaaaa
      241 aaccgaaacc gaacggactg gattcccgcc tgcgtgggaa tgacgggttt caagattacg
      301 gtattgtcgg ggatgatggg aaacggtggg aattgtgtaa aaaatgccgt ctgaagcctt
      361 tcagacggca tttggacgtt taaaacttca tttccaagct aaatgtgtag tttcggccgg
      421 gggcggcata tcggttgtaa acgccgacat ttttgtgttg gttgactgcg ccgccggcag
      481 tttgccgcac attttcccaa gtaacatagc ggtagttgag gaggttgtac acgcccgcac
      541 ggagggtgaa gtgtttttta atcgtgtaat aaccggacac atccacaata taccaagggc
      601 gggtacggcg cgcggtggct tttgtattgc ggctgttgcc gttgagcaaa gcacggctgc
      661 ccaacaactc tgtgatttcc ttggctttgg aataagtcag cataccgttc acaccccatt
      721 tgccttccgg ttggtcatag cccaagccga cgacatagcg cgagggttgg atggcatcaa
      781 acaggtgtga ttgaatatcg gtgcggtctg cgcgtttttt gatgtcgcgg acatggacac
      841 gattataggc aaatgtagaa taccaacctt cgggcaattt atcccatacg ccgttccaat
      901 cgattttgcc caaaacattg atgccggtaa tccgcgcgct ttgggcattg aggtaagccg
      961 ggtcgccttt ggctgtttct ttgccgtttt gaatttgcgc ttcataaccc cggacaatca
     1021 aatcgcggta ggcattgttg aaccaacttg cctccaagtt gccgaaatcg cctttaaaca
     1081 cgatgccggc ttctttgttg aacgattttt ccggatcgat tttgaccgcc ttgctttgaa
     1141 cacccgcccg ccagccgtac atttccgcaa acgagggcag gcggaagccg gttgaagtgc
     1201 ggtaagtcaa atccagccag tcggcaggtt tgaggacgat gcctgcgttc caggacaggg
     1261 tgcggtgcgt gccggtggaa acgctgctgt cgtccgaatg cgtgctgcgg tagtcgtagc
     1321 gcaaccccgc gccgacatcc gcccacctgc ccaaacggac attgtcccga actgccgcgt
     1381 aatagctttt gccgttgatg ctgcgcggcg tgcagtccgt ataagtattg ttgcccaagc
     1441 ggcagatttg ctccgtaacc acatttcccc cgcctatgct gacccaatag ggtttgctct
     1501 tgtcgccgtt ggggctgttt tgattgccgt ttttaggggg cgttttcaac gaataggcgc
     1561 gtttggcgtt ttgaaaataa taatcctgat ggcggagatt agagccaaag cggtcaaacc
     1621 cgagattcac gctcaggttg tggcggattt tggcggtatc gaaggatttt ttgaatgccg
     1681 cctgcaagag cctgtggctt tccccgtaaa tcacgcggtc ggatttgtaa taggaaaacg
     1741 gcttgtcggc actcggacgg caatttttgt ccgaaccgtc ggcagaacag tgcgtctgct
     1801 gaaaatgatt gtccaaaccg ataccctgcc ggtcataaga gaggcgggca taatccgccc
     1861 aagtgtcttt atcggcattg gtatagacat attccaaacc gtagcggctt ttggtgtgcg
     1921 tctcgtcgta aaacacgcct gtaccgtatt ccgcgcccac cagcgcaccg ttttcgccgt
     1981 tggtaaacag tccgccgtat ttgtggttgc ccgcatattt gccgttgcct cttatcggtc
     2041 cgtattttct attttcatca aaaaccgcct tggtcaggaa tgccggaacc gtcatatcgc
     2101 gcgtgtcgaa agtttgttgc gtgtgttcga gtatgccgcc gatgtagtgc cgtttgtttt
     2161 caaaacgaaa acccgggcgg aacaaccacg atcggctttc gtatgaaagc ggatcggcga
     2221 ggaagcggtt gggacccgtg tagtctcggg tggaaaccgt ttgccgttcg tctttgccga
     2281 caacctcttt tttcggattc gctttacacg tttcgtaatt tctgttttcg cattcttcct
     2341 caacgatgaa atttgcgtac cgattggtat cctcaaccgg caccagcctg ttaaagctct
     2401 gaacgccgcg tccggcatct tcgtgtgcgc ggatttcccc cgcgcgccgc ccggtgtgga
     2461 tcagcaaagc ctccgcaccg ccgatgcgcc ccgccagcgc gatggattgg gtaagccccc
     2521 ggtttttgcc ggaataggcg gttttactct gaatgcccca ctgcctgcct tccccgataa
     2581 catcgtcggc ggttttggtt tgaaatgcga ccgagcccgc caatgcgccg ctgccttgtt
     2641 cgaccgagtt tgagcctttg ctgatttcga cagctttgac gttttcatac tcgatttcat
     2701 tgattgcgcc gctgctgccc gccgtcctcg tcccgcccaa tgccgcctgc gcggtgtagg
     2761 actgtatttg cgccaagccg tccaccgtca aggaaacgcg gtttttgtcc ataccgcgta
     2821 tcgagtagcc agagcttgcg ccgcgcccct gttcgaccac ggcgatgccg gggtcgtaac
     2881 gcgtcaggtc gcggatatcg agtacctgtt ccttgctgag ggtgtcggcg gttttgacca
     2941 atttgcccag accggttact tcgttatcgc ggcgggtttt ctgttttttg gcttttacct
     3001 gtatggtatc caactgtttt tcctgtgctt gtccggcttg cacattttct gcataaacgg
     3061 gcagcgcagt catcagcgac aggcataaaa tatttaatcg gaacaaatgt tgctgttgca
     3121 taatgtttcc ctaatcttcg ctttcagacg gcatcggaag gagcgatgcc gtcgaagcct
     3181 tattcttgat tgttcggcaa ccgtgcttat tgcacaggct gttggcgttt cgcaccgaat
     3241 acgacagttg cactgcttgc tgaatttcca ttgccggatg caactgttgt attttccgtt
     3301 tgtttatcgc ccgaataggc aaaccatccg cccaactctt cggctttagg tccgtaaaaa
     3361 ccgccctgca ccttggcgtt tgtgatatat gccttaggcg tgccggtggt attgctttga
     3421 tcgagatcaa aacctaattc agcagttttt gccgtacctt caaagccgtt gtccttaata
     3481 ttagccgtga tagtaaaggt tggctgagtc ctatcattag cggttaacgt gccggtaatt
     3541 tttttatcgc caaaattcac ggtaaagtcc gccctgttgc cactcgttgc attggaagca
     3601 ttgccgctcc agcttgtgct tgtgttgttg gcaatatgcc cgtaccaaga cccccgataa
     3661 acgatgtctt gttgctcttt tggaatctct ttttcatcgg tgcgctcgcc ttggaggaac
     3721 atactttgtt caacttgttc cgttttagcg tcagcttgac taccgttttc gcctgcctgc
     3781 atcgcggact tgctgttttt acgcgtcaac attccgtatt tcagataatt gaggttggaa
     3841 cagcagactt caacttcata ggtttttgtt cctgcctgcc cgccgttaac gcccgccgca
     3901 cccgaagcag cttgcgcgcc gcccgcgcct gtttgggctt tggtgtcttt tttatcactt
     3961 tccagcgtgt aggttgtttt gtaggtaaag gctgttccgc cgtttgtacc ttgattggct
     4021 tgatttttcc cactttcgga agcctcgggc aggagcggaa tcataatgcc gtcgacaacc
     4081 agttgggcgg cattgctgaa gttgtcgaga tttttgattg ctgtgccgcc gtgcgtcagc
     4141 tcgaccgcat ccaaaaccgt ggtcagctta ccgtttttag acgacgtgcc tgccgcaccg
     4201 tccgatgctg ccgcacctgt gccgcctgaa gccactgcgc catttctcgg tttgtcttgg
     4261 gttttcgcgc tgccgacaac ggcaactttt tgatcgttgc tcaaaaagcg gaaacccaat
     4321 tcctcaccct gcgggccgaa aaagccgccg ctcaaagaag acgagtcgga aacaaaggga
     4381 tgttgtttgg tttcattctc ttgtggtttg tcggtcgcta ttgccttgcc attgaagcgg
     4441 ttgcctgtta cttgagcctc aaggctgtag tattgcgtgg taggtttgtc gttattagta
     4501 ttactttggc tcgcattatt gcgtatcaat ttacccgtca atttcttatt gccgaaatcc
     4561 acttctaaat tcgaggtaaa tccataaccc tcgtgaccat ctttcagcgt ggattcgttt
     4621 ttgttggaat attcttcgcc ctcatcacca gaaaatccgc tgtagctgtc gccttgcttt
     4681 tttgaaggct ggataatatc gttaaatttt tgtcccagtt tcgtatcggt tacaaaatgc
     4741 catacgccct tgtaggtaac ttttccagaa gcaggaagtt gtcgggaagg gtctttaccg
     4801 tgataaaaga tatagccgtc gtcgcctgac ttaaattttc cttcattgaa ttttctttca
     4861 ctcgccgcat gtttataaaa ccaaccggaa taaacatatt tgaaattgtt gtaacctgtt
     4921 gcttcgtttt ttggttggtt tgcaccgcca ttgatactgc cgttgtgaga attatggctt
     4981 gattgcgtga gataagggga aatgtaaata ttgttgtcgc cattatctgt ttgtacgttt
     5041 gaaatgacgg attcttgttt taatggcagt ttcttgggtt ctgtcggcaa tcctgtcgcc
     5101 tcccaatcat cattctttaa tttaacctca ttttctttag gcattgccgt aggatgccga
     5161 ttcctccgtt tcaacctcat cgcaaaaccg tatccgcctt ggtctttttg ggcttgcggt
     5221 ttttcggaag aaacatcttg atactttggc gcgggacgcg gggcttcggt atcgacagaa
     5281 tcaagatcga agctgccgcc gccgcccaaa caagcactca acaaaaacac aggcagcacc
     5341 atagcagcct gatttaccaa tggattgttc atataaaccc aattcaatta aagaatgata
     5401 aggattatta ttttatttat ttttaacaaa tttgcaaata cttttttatt tttttaggga
     5461 atacaccaaa atcccgtcat tcccgcgcag gcgggaatcc gaacgcgtcc gcacggaaac
     5521 ctatatcccg tcattcccac gtatatcccg tcattcccgc gcagg
//
LOCUS       DAWFKN010000113         5409 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFKN010000113 DAWFKN010000000
VERSION     DAWFKN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.113"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..1761
                     /locus_tag="WGR46_001993"
     CDS             <1..1761
                     /locus_tag="WGR46_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0856851.1"
                     /translation="LQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKS
                     SIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIPQGNLKT
                     QIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTEAGAAIIALAVTVVT
                     SGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLV
                     VAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEAN
                     ILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEI
                     VGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVEN
                     NAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLDWND
                     AKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQL
                     AQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNM
                     DGSLNQMKTGAAKGRKLNLK"
     gene            1770..2147
                     /locus_tag="WGR46_001994"
     CDS             1770..2147
                     /locus_tag="WGR46_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856852.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            2425..2799
                     /locus_tag="WGR46_001995"
     CDS             2425..2799
                     /locus_tag="WGR46_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856853.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2834..4429
                     /locus_tag="WGR46_001996"
     CDS             2834..4429
                     /locus_tag="WGR46_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0856854.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGDVGKTLKELGRSRTVKKLVV
                     AAAIAGVSNKIGASSLATWSETPWVNNLNVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIV
                     GEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNN
                     ALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISR
                     GGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIK
                     HVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTK
                     GETKVRIVVQKGTNKIISAYPQK"
     gene            4448..4711
                     /locus_tag="WGR46_001997"
     CDS             4448..4711
                     /locus_tag="WGR46_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856855.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            4818..5409
                     /locus_tag="WGR46_001998"
                     /pseudo
     CDS             4818..5409
                     /locus_tag="WGR46_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
BASE COUNT         1719 a         1084 c         1305 g         1301 t
ORIGIN      
        1 ctgcaatcag gttgggatac caaactgcaa ggcacacagt ttgaaaccac actgggtggc
       61 gcaaccatac gcgcaggcgt aggcgagcag gcacgggcag atgccaagat tatcctcgaa
      121 gggatcaaaa gcagcatcca cacagaaacc gtgagcagca gcaaatctac tctatggcaa
      181 aaacaggcag gacggggcag taacatcgaa accttgcaat tgccgagttt caccggtccc
      241 gttgcgcccg tactgtccgc acccggcggt tacattgtcg atattcccca aggcaatctg
      301 aaaacccaaa tcgaaaccct caccaaacag cccgagtatg cttatttgaa acaacttcaa
      361 gttgcgaaaa acatcaactg gaatcaggtg cagcttgctt acgataaatg ggactacaaa
      421 caggagggct taaccgaagc aggtgcggcg attatcgcac tggccgttac cgtggtcacc
      481 tcaggcgcag gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat
      541 gcagcattcg cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcaat
      601 atcggtaaca ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc
      661 gccgctaccg caggcgtagc cgacaaaatc ggcgcttcgg cattgaacaa tgtcagcgat
      721 aagcagtgga tcaacaacct gaccgtcaac ctggccaatg cgggcagtgc cgcactgatt
      781 aataccgctg tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct
      841 ttggtgaata ctgcgcatgg agaggcagca agtaaaatca aacaattgga tcagcactac
      901 atagtccaca agattgccca tgccatagcg ggctgtgcgg cagcggcggc gaataagggc
      961 aagtgtcagg atggtgcgat aggcgctgca gtcggtgaga ttgttggtga ggctttggtt
     1021 aaaaataccg attttagcga tatgaccccg gaacaattag atctggaagt taagaaaatt
     1081 accgcctatg ccaaacttgc ggcaggtaca gttgcaggcg taacgggagg agatgtcaat
     1141 actgctgcac aaaccgcaca aaacgcggta gaaaataatg cggttaaagc tgttgtaact
     1201 gctgcaaaag tggtttataa ggtagccaga aaaggattaa aaaacgggaa aatcaacgtt
     1261 agagatttaa aacagacgtt gaaagacgaa ggttataatt tagccgacaa cctgaccacc
     1321 ttattcgacg aaacattgga ttggaacgat gccaaagccg ttattgatat tgtcgtcgga
     1381 acagagctga atcgcgctaa taaaggggaa gcggcacaaa aggtcaagga agttttagaa
     1441 aaaaatcgtc cttatatccc taataaaggt gctgtaccga atatgagtac atacatgaaa
     1501 aataatcctt ttggaaaaca gctggctcaa atttcagaaa agacaacgct tccgacgcag
     1561 caagggcagt ctgtcttctt ggtaaaaaga aaccaagggt tattaaaaac cggtgatagg
     1621 ttttatttag atggccaaca taaaaatcat ttagaggttt ttgataaaaa tgggaacttt
     1681 aagtttgttc taaatatgga tggttcgctt aaccaaatga aaactggggc agcaaaaggt
     1741 cgtaaattaa acttaaaata ggaaatttta tggaaacatt gaatgatata aaaaaaatct
     1801 tgattaatgt ggggctttat caagggtttg atttgacaga tccaaaagta tcagaagaag
     1861 ttaatcatga aacagctaat atgaaatgga ttaaagatta tacttcagac gggaattggg
     1921 ataatgaatt taaggaggat ttaaaaaact ttttagatta tatggaagta tgccaattag
     1981 ccctaaacga taaaaatttc aaaattgcca gtaattcttt atttatggct atgatttacg
     2041 caggtaatct atctcttata tttgattcaa taaaaactga tatatcaaca ttattgagtg
     2101 ctgagtataa aaagaatagt ttttcatggc catctcttga tgaatagaaa gcaagttgta
     2161 gcctgcatga aatctaaaac ccatgcataa ggtgtgggct tcagtatacg cgttccatga
     2221 tttacggcca tatgccgtct gaaaagctca attttttcag acggcatttg ttatgaaagt
     2281 aaatatttag attccctgta tactgtttag actcgtgtgt gctgagtaag ctgtagtctg
     2341 catgaaacct aaaactcgct caaaattaag ctaagacatt agcagggcaa gggcgaaaat
     2401 tgaatcttaa ataaggtgat tcagatgaaa aattttaatg tagtaaaaga aagtttaaga
     2461 gagttaggaa ttaaacaagg atttgatctt tatgagaaag ccacaactga aaaattgaat
     2521 agtgaagatc ctcttgactt acaatggctt tctaactatt catctgattg gaatgatgaa
     2581 ttagaagaag actttgattc tttttttcag catatgaagg aatatcaata tgctattgac
     2641 aatgaagaca ttaaatctgc atgtagttca ctatgtgaag ctatgctcta tgttggtaat
     2701 attaaaaatt tttttgagtt tctcaaaagc gatatgatta gactgttgag aggtgaaagt
     2761 aaaacaacag actttcaatg gccgcaattt gatgaatagc agcaagctgt agcctgcatg
     2821 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcacgc acgcgttcca tgatttacgg
     2881 ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat
     2941 tcagattccc tatatactgc ccagatgcgt gcgtgctgaa gacaccccct acgcttgcta
     3001 tttgaaacag ctccaagtca ccaaagacgt caactggaac caggtacaac tggcgtacga
     3061 caaatgggac tataaacagg aaggcttaac cggagccgga gcagcgatta ttgcgctggc
     3121 tgttaccgtg gttactgcgg gcgcgggagc cggagccgca ctgggcttaa acggcgcggc
     3181 cgcagcggca accgatgccg cattcgcctc gctggccagc caggcttccg tatcgctcat
     3241 caacaataaa ggcgatgtcg gtaaaaccct gaaggagctg ggcagaagcc gcacagtgaa
     3301 aaaactggtt gtagcagcgg ccatcgcagg cgtatccaac aaaatcggtg cttcctccct
     3361 tgccacttgg agcgaaaccc cttgggtaaa caacctcaac gtcaacttgg ctaatgcggg
     3421 cagtgccgcg ctgattaaca ccgccgttaa cggcggcagt ctgaaagaca atctggaagc
     3481 gaatatcctt gcggctttgg tgaatactgc gcatggagag gcagcaagta aaatcaaaca
     3541 attggatcag cactacattg cccataagat tgcccatgcc atagcgggtt gtgcggcagc
     3601 ggcggcgaat aagggtaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     3661 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     3721 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     3781 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     3841 agatattatt tgggatatcg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     3901 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     3961 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     4021 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     4081 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     4141 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     4201 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     4261 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     4321 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     4381 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     4441 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     4501 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     4561 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     4621 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     4681 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     4741 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     4801 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcacaag cacgagttgg
     4861 atgtccaaaa aagccgccgc tttatcggca tcaaggtagg taagagcaat tacagtaaaa
     4921 acgaactgaa cgaaaccaaa ttgcctgtcc gcgtcgtcgc ccaaactgca gccacccgtt
     4981 caggctggga taccgtgctc gaaggtaccg aattcaaaac cacgctggcc ggtgccgaca
     5041 ttcaggcagg tgtaggcgaa aaagcccgtg tcgatgcgaa aattatcctc aaaggcattg
     5101 tgaaccgtat ccagtcggaa gaaaaattag aaaccaactc aaccgtatgg cagaaacagg
     5161 ccggacgcgg cagcactatc gaaacgctaa aactgcccag cttcgaaagc cctactccgc
     5221 ccaaattgtc cgcacccggc ggctatatcg tcgacattcc gaaaggcaat ctgaaaaccg
     5281 aaatcgaaaa gctgtccaaa cagcccgagt atgcctatct gaaacagctc caagtagcga
     5341 aaaacatcaa ctggaatcag gtgcagcttg cttacgacag atgggactac aaacaggagg
     5401 gcttaaccg
//
LOCUS       DAWFKN010000114         5364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFKN010000114 DAWFKN010000000
VERSION     DAWFKN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.114"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(158..2992)
                     /gene="lbpA"
                     /locus_tag="WGR46_001999"
                     /gene_synonym="tbpA"
     CDS             complement(158..2992)
                     /gene="lbpA"
                     /locus_tag="WGR46_001999"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0856856.1"
                     /translation="MNRRHTFPLTLTALAIAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDILSPNKKAEYFLAEGESEL
                     KPVAKLAGNGNYLKNQLNRWVEERRSKGQALNSEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SDKSGVYDGNDFRDGLYFRPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEST
                     GFDDDNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSAKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2989..5148)
                     /locus_tag="WGR46_002000"
     CDS             complement(2989..5148)
                     /locus_tag="WGR46_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0856857.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNG
                     PYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKQKTDAANASDTKPALTSGKHTKILDSLKISVDEAADE
                     KARPFEVSTMPDFGHPDKLLVEGREISLLKDTQTIVLADGRTMTVSACCDFLTYVKLG
                     RIKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDEDE
                     EEEPEETEETEETEETEETEETEETEETEETEEKSPTEEGNGGSGSILPTPEASKGRD
                     IDLFLKGIRTAEADIPQIGKARYTGTWEARIGEPIQWDNKADKAAKAEFNVDFGEKSI
                     SGTLTEENGVEPAFHIENGKIEGNGFYATARTRDNGINLSGTGSTDPKTFKANDLRVE
                     GGFYGPKAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1127 a         1458 c         1263 g         1516 t
ORIGIN      
        1 gataaattgc cttagcattg aatgtctgga ttcccgcctg cgcgggaatg acggcggaaa
       61 tcttgtttat attgaatcaa aaaaacctgc accttaatca gttggcggtt tagtccgact
      121 tttggggtgc agatcaagct ttcagacggt atttccttta aaacttcatt tcgagcgcga
      181 ggctgaagtt tctgcccggt gcggcatacc ttccatagtt actgtcgccg ccgtgccggt
      241 ttgccgtgct ttccgcagtc tggcgcaagg attcccaagt aacgtagcgg tagttgccga
      301 tattgtagat agccgccctc aaggtcagcc gttttttcag attcagatag gcggaaacgt
      361 ctgccgtcga ccaagaagac gacgctcttt ttgccgaata tcgtttttga tcgcctgcca
      421 gataagcaag ctcgtcaggg tttttccctt tggaataggt cagcataatg tttgcgcccc
      481 atttcccctc gggctggtcg tatccgaacc ccaaaacata acgcgacggc tgtaccgcat
      541 ccaaagcata gctgcggagg gacagtcccg gccggttgga taccgatttc ggtttgatgc
      601 ggttgtacgc caaagtcgtg tacaaacctt cgggcagttt gccgtacaca ccgttccagt
      661 cgatttttcc caatatatta acgccttgaa gcgacatatt ttgggcattg taataatcgc
      721 gtatatcaat ctctgtcaat tgtcctgcct gattcggcaa tttggttttg tgatcggcaa
      781 cggcaatcat atcggtatag cggttgcgga agctgctgat ttccaaaaag ccgaagtcgc
      841 ctttccactg caaaccgatt tcccggttgg ctgccttttc cgatttcagg gcgggacgct
      901 gccagccttt cggataatcg tgataaatgt ctatcccgaa aagttcttgg aatgagggcg
      961 ttctgaagcc gctggaggcg cggtaagaca cggaaaaatg ccggttcggt ttgaacacga
     1021 tgccgctgtt ccacgaacgg tcaacatacc gtccgctgcg gacgagttct tccgacgtgg
     1081 tgaagttttt ccggtcatac ctgccgccca agctgaaatc gaaatatttg ccgattgaaa
     1141 aacggtcgtt caaagaaatg tggatattgc tgccgttgat ttttcttggc acgcatttgc
     1201 gggaacgcag ggtttcgatg tagccgcaga ccgacccttc gacgacttcg ggcttaccca
     1261 aacggtactt atcttgattg tcatcatcga atcccgtgga ttccgaaatc cttgccgcat
     1321 tgtgggaaag ctgttcgggg cgggagatcg ctttggaagc atcgtaaccg aagcccaaag
     1381 tcagatggtg tttcgtccat ttgtttttca gcgatttctc aaacgaggca ttcaaaacat
     1441 tgtgctgttc gcggtagtgg aaacggtcgc tgctgtcgta ggaatacggt ttgtccgccg
     1501 acgcgcggca ggatttgtcc acagcaggat acacggcgca attcagcttc agcgtgttgt
     1561 tgtcggttgc cacgccctgt ttgtcaaacg acaacaccgc cttatccgcc cagttgtcag
     1621 aatacgcttc gttttcataa cggtacagca aacccatacg gcggcggcgg tgatgttcgt
     1681 ctataaattt ggtgcgggaa tatttcaaac ctatgccctt gaccaaattt gtatcgccct
     1741 tccactcttc tatattcggc ctaaaataca agccgtcgcg gaaatcgttg ccgtcgtaca
     1801 ccccgctctt gtcgctaaac tttttcgcct catccgtacc gtaatactgt ttttccgtca
     1861 tatcgcggat gtcgtaacgc tgtttggtat cctcaaacac gccgccgaca taatgcctgc
     1921 cgccgaagcg gtagcccagc ttggcaagcc acgaaccgct gcggtaatcc atcggatcgg
     1981 gcaatatcct gccgccgccc gtgtaagctt gggcggacag gttttcgtgg cgcgcctgcg
     2041 cctcccgcac catcgcttct tcttcactgt ttaaagcctg accttttgaa cggcgttctt
     2101 ccacccagcg gttgagctgg tttttcaaat agtttccgtt gcccgccaat tttgccacag
     2161 gcttgagctc actctcgccc tccgccagga aatattccgc ctttttattc ggactcagaa
     2221 tatcgtatgt ctggcggaac gcgtccaaac ggtttatgcc gtatgccacc ccgtccgcaa
     2281 tatcgccgtg cgggcgcgtt tcccgccctt ggcgttcggt tcggattagc agcccttccc
     2341 aaccgtcttt gctgaacccc gcgccgagcg acttcataaa ttggcggttt ttactgccgt
     2401 aggcagtttt tgcctgtatc ccccaacttt tgccgtctga aatcaggtct gccgcttctt
     2461 tggtgcggaa ggcgaccgcg ccgccgagtg cgccgctgcc gtgatcggac gaaccggcgc
     2521 ctttgtcgat ttccaccgtg ctgatgtttt catattcgat ttcgttgatt gcaccgctgc
     2581 cgccgcgtcc gccgtatccg ctcaacgatc cctgcacggt aaacgcctgt atttgggcaa
     2641 cgccgtcaac cgaaaccgcc acacggtttt tatccacgcc gcgtatcgag tagccgccgc
     2701 tcgcgccgtt gccctgttcg acaaccgcca cgcccggatc gtagcgcgtc aggtcgcgga
     2761 taccgagtac ctgttctttg ttcaacgttt ccgacgtttt gacgattttg cccaaaccgg
     2821 tcgcctcttt cgatcgccgt cccactttgg cggcacggac ggtaacctct ttcaaggatt
     2881 gggtctgcgc cgcatcaggt gccgccgttt cagggtttgc cgcatatgac gggaaggcgg
     2941 cggcaatggc caaggcggtc agggtaagcg ggaatgtatg ccttctgttc atcgtgttgc
     3001 ctccttgtca tctttcttcg caccgaatac cgcaccgaat ttccggtcgc tgtcgataat
     3061 attaccgccc aattccgccg ccttcggacc gtaaaatcct ccttctacac gaagatcatt
     3121 agctttgaag gttttggggt cggtcgaacc agttcccgaa agattgatgc cgttatcccg
     3181 agtgcgcgct gtcgcgtaga aaccgttgcc ctcaatcttg ccgttttcaa tatggaaagc
     3241 aggttctaca ccgttttcct ccgtcagcgt tccggaaatc gatttctcgc cgaaatcaac
     3301 attaaactct gcttttgccg ctttatccgc cttattgtcc cattgaatgg gttcgccgat
     3361 acgcgcttcc caagtgccgg tatagcgtgc ttttccaatt tgcggaatgt cggcttccgc
     3421 cgtgcggata cctttcagga aaaggtcgat gtccctgcct ttagaggctt ccggagtggg
     3481 caggatgctg cctgaaccgc cgttgccttc ttctgtcggc gatttttctt cagtttcttc
     3541 agtttcttca gtttcttcag tttcttcagt ttcttcagtt tcttcagttt cttcagtttc
     3601 ttcagtttct tcgggttctt cctcttcgtc ttcatcagtt tcttcttcgg tttcttcagc
     3661 ttcctcttct tcggtgactt catcttcgcc gttatcatct tcggaaattt cgtcttcgct
     3721 ttcttcgcca ttatcaatgt ccgaatcctc ttcatcctgc gccttcggtt tgacggcggg
     3781 gcgttcggtt tttatccgtc cgagtttcac ataggtcaga aaatcgcaac aagcactgac
     3841 ggtcattgtc ctaccgtcgg caagaacgat ggtttgagta tctttgagca aggagatttc
     3901 acgcccttcg acaagaagtt tgtcgggatg accaaaatcg ggcatagtgg aaacctcaaa
     3961 cggacgggct ttttcatcag ccgcctcgtc aacggaaatt tttaaagaat ccaagatttt
     4021 ggtgtgtttt ccagacgtca gggcaggttt tgtatctgat gcgtttgctg cgtctgtttt
     4081 ttgtttgcct gcgaatacgc cgaatacgct gttgtcgttg ctgataaacc gtccggcaag
     4141 ctcttctccg ttatcgccga aaaaaccgcc ctcaagccgc tgatcggcat cgtcatggaa
     4201 aaacaaatgc tctttatcag cgtggctcgt cttcaacttg gtgttaactt tggcactgcc
     4261 ggtaaagcgg ttgccgtcca atgttgcagt aatgtcgtaa atggtcagcg gattataggg
     4321 accatttcta tctcgcacat actgattttt aatcagctcg ccctttagga tttttttgtc
     4381 gaaatcaacc gtatatttgg caggatgctt ttccctttcg tcgacatcct tagccgcata
     4441 agaagttgca ccaacatcgt taccataaaa tgtgatataa cccaaattgt caatgccaac
     4501 cgccttacct gctcgatgac gtttgacatc ggtcatatat tgccagttac cagaatattc
     4561 caccgttccc gcactcggta aagattggga aggacgttct ccggaataat atacaaaacc
     4621 gtcataacct aagcggttgg aaaactgctt accacctgag ttctgttcga tttcatctgt
     4681 tccaaatcta gtataaacat atccggcccg gacatgttca tatttataca cagcatcttt
     4741 taaatcggac gtgtagattg ttacattagg ataccgtccg ttgatttcct ttttaagttt
     4801 gtcagcctgt tctttcggcg taccgtctaa aaacaggata tcctcctctt tgagcggcag
     4861 atactcctct gcctgcttac tatcgggaat ttcattacca tttttatcaa aagctgcgat
     4921 attccgcctt ggcagccgca ttgccgcacc gacggcgggc cggttgaccg gcgtggtttc
     4981 taccgaaggc ccggcagggg gcgaagtggg aacgtcctta gacttgaaag tgacggggta
     5041 cgcggtcggc gttgattcga caacaggctg cacgccgaaa ttgccgccga tgcaagatgc
     5101 caaaagtaag ggcaacaaga caatgccgcc ataattcggt ttacacatcc ctacttttcc
     5161 tcctatttga ttaataataa ttatcattat attaatatgt acagataata tcaacccgtt
     5221 tttatagtga attaacaaaa atccggacaa ggcgacgagc cgcagacagt acagatacat
     5281 cccgtcattc ccacgaacct acaccacgtc attcccacga acctacattc cgtcattccc
     5341 acgaacctgc accacgtcat tccc
//
LOCUS       DAWFKN010000115         5295 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFKN010000115 DAWFKN010000000
VERSION     DAWFKN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.115"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(99..1016)
                     /gene="thrB"
                     /locus_tag="WGR46_002001"
     CDS             complement(99..1016)
                     /gene="thrB"
                     /locus_tag="WGR46_002001"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0856858.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="WGR46_002002"
     CDS             complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="WGR46_002002"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0856859.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1933..3174
                     /locus_tag="WGR46_002003"
     CDS             1933..3174
                     /locus_tag="WGR46_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0856860.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            complement(3244..4896)
                     /locus_tag="WGR46_002004"
     CDS             complement(3244..4896)
                     /locus_tag="WGR46_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0856861.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            5066..>5295
                     /locus_tag="WGR46_002005"
     CDS             5066..>5295
                     /locus_tag="WGR46_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0856862.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSD"
BASE COUNT         1330 a         1584 c         1228 g         1153 t
ORIGIN      
        1 gaaaataccc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg ccgtctgaaa
       61 ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aatccaaact caacagcagg
      121 ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc ccgcctgcgg aaaatgaaag
      181 tccaacaggc gcgacaccca aaaacggatg cagccggcac gttgggcggt cgggaaatac
      241 gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa cacctttttc
      301 aacgcctcat ccaacttatt gtccgccgtc cttgcccaat cgttgaccgc aatcgccaag
      361 tcatacataa aattgccccg gcaggcataa tagaaatcga tgaaacccga tacctgaccg
      421 ccgtcaagca acacattgtc tttaaacaaa tcggcatgga tgatgcccga aggcagatga
      481 ttgccgagat tgtcctttaa cgcatcgatt tcggaacaca gcagcgcggc atcgtcttgc
      541 gacaggacgg gcagcagccg ggcgcacgcc tccgtccacc acgcattgta acgcgggttt
      601 tccatttcca aagggaaatc ggcggcggca aggtgcattt tcgccaacat cgcgccggta
      661 tggaaacact gctcagccgt cggcagcgcg gtatccgaac ctttcaggca ggcaaccaaa
      721 caagccggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc aaccggcgcg
      781 gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa cggcagctct
      841 tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt cgtcagaaaa
      901 taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc acccaaatcg
      961 taaccgctca ggaagccgcg catttcatca tcggaaacac tggtatagac agacatatca
     1021 actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg ccggacggca ggcaaacata
     1081 taattcacat cggtcgaatc gcacagggca taagtttgcg acaacacatg gtaagtcata
     1141 cccttcgtat ccgccacatc caagcctgcc tgacggcaca tccgcgccag ctcggcaggc
     1201 gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa acttcaacag atattccgcc
     1261 gccacaatca gatgcaggta cgatttcggg tttttattga tggtggaaaa aaacaccatg
     1321 ccgtccggtt tgaccagatt ggcacaagca cgcacgatgg cggcgggatc ggggacgtgt
     1381 tccatcattt ccatgcacgt taccacatcg aacgagtgcg gttccgcctc ggcaaggtct
     1441 tccacgcgga tacattcgta ttcgatatcg gcgacattgt tcaaagccgc gtgcaggcgg
     1501 gcggtttcca gcgactgctc cgccatgtcg atacccttta caaacgccgc gccgcgccgt
     1561 gccatacttt ccgccaagat gccgccgccg cagcccacgt ccaaaacccg tttgccgcac
     1621 aaatccgcgt gcccgtcgat ataatccagc cgcagcggat tgatgtcgtg caaggttttg
     1681 aactcgcccg acttgtccca ccatttgtcg gcaatccggc tgaatttggc gatttccccc
     1741 tcatcgacat tatatttttt gtcggacatt ttccctccca tctgacgaac cgcccactcc
     1801 aaaacccaag attatactat ggaacgcaat gccgtctgaa agccttttcg ggcggcgcgg
     1861 cgcaaatacc ttacaaatcc ttacacttta cggcataatg gcggctcgct ttttctggca
     1921 gaaagacaaa atatgcccac caaaacccct tcactgttcg gcggcgcgat gattatcgcc
     1981 ggcacggtca tcggcgcagg tatgctcgcc aacccgaccg ccacatccgg cgtatggttt
     2041 accggctcgc tggccgtgtt gctgtacacc tggttttcca tgctctccag cggcctgatg
     2101 attttggaag tcaacaccca ctacccccac ggcgcgagct tcgacaccat ggttaaagac
     2161 ctgctcggac ggagctggaa catcatcaac ggcatcgccg tcgccttcgt tttatacctg
     2221 cttacttacg cttatatctt cgtcggcggc gacctgaccg ccaaaggctt aggcagcgcg
     2281 gcaggcggca atgtttcact caccgtcgga caactcgtct tcttcggcat tctcgccttt
     2341 tgcgtatggg catccgcacg cttggtcgac cgattcacca gcgtcctcat cggcggcatg
     2401 gtattaacct ttatttgggc aaccggcggc ctgattgccg atgccaaact gcccgtcctc
     2461 ttcgacaccc aagcccctac cggcaccaac tactggattt atgtcgccac cgccctgccc
     2521 gtctgccttg cgtcattcgg tttccacggc aacgtctcca gcctgctcaa atactttaaa
     2581 ggcgacgcgc ccaaagtggc taaatccatc tggacgggca cactgattgc gctggtaatt
     2641 tacgtcctct ggcaaaccgc catccaaggc aacctgccgc gcaacgagtt cgcccccgtg
     2701 attgccgccg aagggcaagt ctccgtcctg attgaaaccc tgtccaaatt cgcccaaacc
     2761 ggcaatatgg acaaaatatt gtccctgttt tcctatatgg cgatcgccac ctcgttttta
     2821 ggcgtaacgc tcggactctt cgactacatc gccgacatct tcaaatggaa cgacagcgtg
     2881 tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc ctctaatttc ctgcctgctc
     2941 ttccccaccg gctttgttac cgccatcggc tacgtcggcc tggcggcaac cgtctggaca
     3001 ggcatcatcc ccgccatgct gctctaccgt tcgcgcaaaa aattcggcgc aggcaaaacc
     3061 tataaagttt acggcggctt gtggctgatg gtttgggtct tccttttcgg catcgtcaac
     3121 atcgccgcac aggtattgag ccaaatggaa ctcgtccccg tatttaaagg ataaaggcaa
     3181 aatgccgtct gaacgagttt tcagacggca ttttcgcaac cgttgcagcc atgcggcaaa
     3241 aagctaacgg gcaattttca aaacgtcgta cagatagttg cggtaatttc cgaccaactc
     3301 ccagttcggg cagtcttcca accctccgcc gcacaaaccg cgcatcgcca tattcgcacg
     3361 catgaagccg tctttggctt ccagaccagc cgcctccatc agcacgccgc ctgcaaaggc
     3421 aaggtcgagg aaattctgcc gctgtacgaa tccgccggca atattgctcc tgacggcaaa
     3481 ctgcgttaca taatccggct gcgcgtaatc ccatttcttc ctgacggaca cgccctcaaa
     3541 cggtacctga tgatcgccga aataaccgaa aacaaagggt ttgccgcgtt cgtgcagata
     3601 acggtcgaaa ctttccaccg ctttgtccaa atcggcaatg cgcccgatgt agtcgttaag
     3661 cgcggatacg gttttcgcat tcaaatcggg cgcatccaaa tcaaacacat tgtcggtatc
     3721 cgtccgatac ggcccgtgct ccttcatggt cagcacatat acgaacatcg gctgccgcac
     3781 gttttccaaa tcgggatggc gtttttccaa aatcatccgc gcatactgca tcatttcctc
     3841 actggaaata tgccacaggt ttttgcccat cggcgcggga tagcccaaat cttgcggctg
     3901 gaacatcaga ttaaagccga aatggtcata tgctgccttg gcattgtagt tgcccttggt
     3961 aaatggcgag agtgccacgc aaaaataacc gtgttcgcgc aggttgcgga caaaaccggt
     4021 ctgcaaatgc ggtacgaccg agtaaaacac gccgcttgcc aacgcgccga aatccgtgga
     4081 cggaacaccc gccaaaaacg caaattcgga cttccacgtt gcgccgccga aagtatgcac
     4141 gcgcaaaggt gaggaaaata cggtatcttc ctgccgtccg aacattttca aatcgggaat
     4201 cttggcggcg gcaaagtcga aacagtgcgg atcgagcgtc gattccatca gggtaacgac
     4261 aatatcgggt ttttcatccg acatgccgca cggccgggtt tcagacggca tctgcctggc
     4321 aaacgcttcc ccgtcgccct cgaacacggg tacttggaaa aacaccgccc gacaggacat
     4381 cggcaggttc aaaaacacgt cgcgcccgtc gtccggcagc gaatccagcc acgtctttac
     4441 cgcgcccgga tgtttggaaa aatgccgcat cgacacgaac gccgccgcaa acaaaaccgc
     4501 gcctgcccac cgccacggca catccaaaga atccgcaccg ctccagccga aaaccgcata
     4561 tgccgccaaa gccagcagcc ccgccatacc gataaccgct tccttataat ggaacagcgt
     4621 ttcccaattc cgccagtcgg caatcagcaa aaagtcggaa atcaacagcg gctgtttgta
     4681 ataatggatt ttcagcctgt ggaacagtat cagcaccaca aaccagaccg aagcgaaatt
     4741 caaggcacgt tgccactgag ccgtcaacat cagcataccg cccgccaaaa agacaaacag
     4801 cgcggatgcg aaaaaatacg tccagcggta gtgcgacctg ataatcagca ccaacgccgc
     4861 cgttacaaac aaaaacagga aaacataggc aaccatcccg ccccactcct caaaacggca
     4921 tacctgagcc gttgcgaatt attatcgaaa gcgtgcatta taatgcagca caggcgcatc
     4981 actgccgata tttcagtata attattcgag atggaatcac tgaaaatggt gaagttacaa
     5041 tgagcccttt tagaggaact aaaaagtgaa ttataaaata tacgatggtg caaagattat
     5101 taaaaaagaa aatttatcaa ttggggatta ttctcaaatt gatgattttg tattttttaa
     5161 ttgtggagag aaaagtattt taggaagttt tgttcatata tcatcattta ccagtattat
     5221 tggtggtgga gaattttata tggatcattt ttcaggatta agcgcagggt gtagaattat
     5281 taccggtagc gatga
//
LOCUS       DAWFKN010000116         5155 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFKN010000116 DAWFKN010000000
VERSION     DAWFKN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.116"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(1..252)
                     /locus_tag="WGR46_002006"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="WGR46_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(324..569)
                     /locus_tag="WGR46_002007"
     CDS             complement(324..569)
                     /locus_tag="WGR46_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856863.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(570..866)
                     /locus_tag="WGR46_002008"
     CDS             complement(570..866)
                     /locus_tag="WGR46_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856864.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(857..1210)
                     /locus_tag="WGR46_002009"
     CDS             complement(857..1210)
                     /locus_tag="WGR46_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856865.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1446..2873
                     /locus_tag="WGR46_002010"
     CDS             1446..2873
                     /locus_tag="WGR46_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0856866.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2942..4345
                     /locus_tag="WGR46_002011"
     CDS             2942..4345
                     /locus_tag="WGR46_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0856867.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYEAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4509..5102)
                     /locus_tag="WGR46_002012"
     CDS             complement(4509..5102)
                     /locus_tag="WGR46_002012"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0856868.1"
                     /translation="MYGSHHLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQPAEFSD
                     YQRVYLDETGFDRYLFRPYALSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSP
                     ELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1306 a         1261 c         1331 g         1257 t
ORIGIN      
        1 atttgatcaa tgaaagccga atctgataca ccgcgccgaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtaccccc ccctgttaga taagggggga aggtttgaag cggttgccgg
      181 cttcctgccg tccgctggcg cgtccgtcat cacgccggca accgcctttg tcatcccttg
      241 cttatcttcc atggtgcgaa tcctcaaaaa cgggcaaaaa aaaagccctg ttacttgtag
      301 aaagtaaagg acgttaattt ttgttaatcg tcccttctta gggacgcaat atataaggga
      361 cgcaatatat aaggttttaa aacgctaaga acaaaatccg ataacggctc tgaagaagta
      421 agtctttctt tattgcacaa gatagcaaac ttccacggca ttctcgcccc acccgtgccg
      481 tccaagcctt tttcaaagac atagatggtt ttcttggcaa tcacttcgtc agtttggcgg
      541 tcgataatcc gtatggtcgc acctgccact cagtgcctgc gcagcttgga atcgacattg
      601 ttttcgaacg ttaccgcata acgggcgagg tgtatatgat taagtatttg atttatcgga
      661 aaatctttac ctgaataccg aataatatcg gaatggttgg gttgcaggac atccacatag
      721 cggtattccc catcgctaaa aaatcctagg aaacgagcaa taaaatttac tccttcgctg
      781 gtctgtaaag ccgcattgtc ccacatcggg tctctggttt ttgcatctgc cgaaacggta
      841 cgctcaggta ctaccttcaa cagcataatc ccttccacat tgtccgccgt ctggtaaatc
      901 tttcccccgc cgttttgcat tgttcgttaa acacggcttc ggcttctttg tattttctgt
      961 cccactcttc ttgtgcctgt atttcttctt tgatcgggcc gaattgtttg ggaataatcc
     1021 aaacaaacag catcaggata gcggcggcgg tcaggctgcc tgaaaggatt ttgccggggt
     1081 tccgtttggg ctttttatag gcaaagcgga cgagaaacca aagcaacagc agcatggtgc
     1141 cccaatagcc gattgagaat aggatggcca aaccttctag gaaatggcgt aaatcgtttg
     1201 tggtaaacat gggttgttcc tgtggttaaa tgtgcaggct gctttttgcc gaatcttgcc
     1261 gcatctcaaa agcagcctgc gcttcagcgt tgcgttacgc agtaaaataa tgaatatttg
     1321 taacggcttg ggtatttttt gtcaatattc ccgcctttcc cttaacagct gccgcgcttt
     1381 ccgttaaaat tcctttacat atttatattg tttcctgttt ctatattgcc aaggttatac
     1441 tcgttatgtt tttctccgcc ctgaaatcct ttctttcccg ctacattacc gtatggcgca
     1501 atgtttgggc ggtgcgcgac cagttggaac cgcccaaacg cacggcggaa gaacaggcgt
     1561 ttttgcccgc gcatttggaa ctgaccgata cgccggtctc tgccgctccg aaatgggcgg
     1621 cgcgttttat tatggcgttt gcgcttttgg ctttgttgtg gtcctggttc ggcaaaatcg
     1681 atattgtggc ggcggcttcg ggcaaaacgg tgtcgggcgg gcgcagcaaa accatccagc
     1741 cgctggaaac ggcggtggtt aaggcggtac atgtgcgcga cgggcagcat gtgaaacagg
     1801 gagaaacgct ggcggaactg gaggctgtgg gaacagacag cgatgtggtg cagtcggagc
     1861 aggctttgca ggctgcccaa ttgtccaaac tgcgttatga agcggtattg gcggcattgg
     1921 aaagccgtac cgtgccgcat atcgatatgg cgcaagcacg gtctttaggt ctctccgatg
     1981 ccgatgtgca atcggcgcag gtgttggcgc agcaccagta tcaggcatgg gcggcgcagg
     2041 atgcgcaatt gcagtcggct ttgcgcggcc atcaggcgga attgcagtcg gccaaggcgc
     2101 aggagcagaa gctggtttcg gtgggggcga tcgagcagca gaaaacagca gactaccgcc
     2161 gtttgcgggc cgacaatttt atttcggaac atgcgttttt ggagcagcag agcaaatcgg
     2221 tcagcaattg gaacgatttg gaaagtacgc gcggtcagat gaggcagatt caggcggcca
     2281 ttgcacaggc ggagcagaat cgggtgctga atacgcagaa cctgaaacgc gatacgctgg
     2341 atgcgctgcg ccaggcaaac gaacagattg accaataccg cggccaaacg gataaggcaa
     2401 agcagcggca gcagctgatg acaatacagt cgcctgcgga cggcacggtg caggaattgg
     2461 ccacctatac ggtgggcggt gtggtgcagg ctgcccaaaa aatgatggtg attgcgcccg
     2521 atgacgacaa aatggacgtg gaagttttgg tattgaacaa agacatcggt tttgtggaac
     2581 agggacagga tgcggtggtg aagattgaga gttttcccta tacgcgctac ggttatctga
     2641 cgggcaaggt gaaaagtgtc agccatgatg cggtaagcca cgaacagttg ggcttggttt
     2701 atacggcggt ggtgtcgctg gacaaacata ccttgaatat tgacggcaaa gcagtgaatc
     2761 tgacggcggg catgaatgtc acggcggaga ttaaaacggg taaacggcgg gtgctggatt
     2821 atctgttaag cccgctgcaa accaaattgg acgaaagctt tagggagcga taggcggatc
     2881 cgtactgggc atttgttatc cgccggttcg gacatgcaga ctgcctgaaa ccattgcccg
     2941 gatgacattg ctcaatctaa tgataatgca agattacggt atttccgttt gcctgacact
     3001 gacgccctat ttgcaacatg aactattttc ggctatgaaa tcctattttt ccaaatatat
     3061 cctacccgtt tcacttttta ccttgccact atccctttcc ccatccgttt cggcttttac
     3121 gctgcctgaa gcatggcggg cggcgcagca acattcggct gattttcaag cgtcccatta
     3181 ccagcgtgat gcagtgcgcg cacggcaaca acaagccaag gccgcattcc ttccccatgt
     3241 atccgccaat gccagctacc agcgccagcc gccatcgatt tcttccaccc gcgaaacaca
     3301 gggatggagc gtgcaggtgg gacaaacctt atttgacgct gccaaatttg cacaataccg
     3361 ccaaagcagg ttcgatacgc aggctgcaga acagcgtttc gatgcggcac gcgaagaatt
     3421 gctgttgaaa gttgccgaaa gttatttcaa cgttttactc agccgagaca ccgttgccgc
     3481 ccatgcggcg gaaaaagagg cttatgccca gcaggtaagg caggcgcagg ctttattcaa
     3541 taaaggtgct gccaccgcgc tggatattca cgaagccaaa gccggttacg acaatgccct
     3601 ggcccaagaa atcgccgtat tggctgagaa acaaacctat gaaaaccagt tgaacgacta
     3661 caccgacctg gatagcaaac aaatcgaggc catagatacc gccaacctgt tggcacgcta
     3721 tctgcccaag ctggaacgtt acagtctgga tgaatggcag cgcattgcct tatccaacaa
     3781 tcatgaatac cggatgcagc agcttgccct gcaaagcagc ggacaggcgc ttcgggcagc
     3841 acagaacagc cgctatccca ccgtttctgc ccatgtcggc tatcagaata acctctacac
     3901 ttcatctgcg cagaataatg actaccacta tcggggcaaa gggatgagcg tcggcgtaca
     3961 gttgaatttg ccgctttata ccggcggaga attgtcgggc aaaatccatg aagccgaagc
     4021 gcaatacgag gccgccgaag cacagctgac cgcaaccgag cggcacatca aactcgccgt
     4081 acgccaggct tataccgaaa gcggtgcggc gcgttaccaa atcatggcgc aagaacgggt
     4141 tttggaaagc agccgtttga aactgaaatc gaccgaaacc ggccaacaat acggcatccg
     4201 caaccggctg gaagtaatac gggcgcggca ggaagtcgcc caagcagaac agaaactggc
     4261 tcaagcacgg tataaattca tgctggctta tttgcgcttg gtgaaagaga gcgggttagg
     4321 gttggaaacg gtatttgcgg aataaagcag gctgaaacgg ttatgaaatt cccaaagcag
     4381 cctgcacccc gtttcgaaag tgcaggctgc tttgggattg atccgatact ttcacattct
     4441 cattgccttt aaataaagca cagttaaaaa cacatttaaa ttaagtgcga attaattcaa
     4501 atgtatattc aattaaaatc aaaataggac agtagtgcat cgtcaaatcg ggcgtaatca
     4561 gacaaaacgg ttcgcagata ccgcttaata ttcgcccaca ctttctcaat cgggttaagc
     4621 tcaggtgaat aaggtgcaag aggcaatacc ttatgtccca atttttccgc catttcccgt
     4681 aagacaccca tacggtgaaa tcgcgcatta tctaaaataa tcaccgattt ttgagtcaat
     4741 gcgggcagta ggcattgctg aaaccacgct tcaaaaaaga ctccggtcat cgtattttga
     4801 taaaccatcg gagcaatcag ccggttgccg acttgtgcgg acaccaaaga taagcgttgg
     4861 tatctttttc cacttatctg cgctttcact atttgccctt tcaggctgag ggcataggga
     4921 cggaacaggt agcggtcaaa tcctgtttca tccaaataaa cacgttggta gtcggaaaat
     4981 tcggccggct gtgtcaaata atgcgttact ttggccgggt cttgttcttt gtaagtggtg
     5041 gtcttttttt gcgcgttatc cccatctgtt tgagtgcata gcaaatggtg gctgccgtac
     5101 aatcaaaatg tttggcgatt tcatgcagat aggcatccgg gtgttgccca acata
//
LOCUS       DAWFKN010000117         4936 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFKN010000117 DAWFKN010000000
VERSION     DAWFKN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.117"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(26..349)
                     /locus_tag="WGR46_002013"
     CDS             complement(26..349)
                     /locus_tag="WGR46_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0856869.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WGR46_002014"
     CDS             complement(337..576)
                     /locus_tag="WGR46_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0856870.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGR46_002015"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGR46_002015"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0856871.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRQAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2302..2487)
                     /locus_tag="WGR46_002016"
     CDS             complement(2302..2487)
                     /locus_tag="WGR46_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856872.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(2598..3290)
                     /locus_tag="WGR46_002017"
     CDS             complement(2598..3290)
                     /locus_tag="WGR46_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0856873.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(3287..4030)
                     /locus_tag="WGR46_002018"
     CDS             complement(3287..4030)
                     /locus_tag="WGR46_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0856874.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGR46_002019"
     CDS             complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGR46_002019"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0856875.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            complement(4619..>4936)
                     /locus_tag="WGR46_002020"
     CDS             complement(4619..>4936)
                     /locus_tag="WGR46_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0856876.1"
                     /translation="NPTVPSKYTNVKKSSVRIGKHAILGTNVTVLPGVTIGDGVAVGA
                     GAIVRKNLEPWTIYVGVDCKPLKRRSQDKILELEKHLVAEFNLDLCPIIPSPTRPNPK
                     ETR"
BASE COUNT         1024 a         1176 c         1492 g         1244 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagatact acgacacaag gacgtgtctt tttgatttcg cttcctacag
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cctctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtccactg cccctatttg acccaataat gatttgggca gaatcactcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatgc gtaccatatt taccagttta
      601 tgtaataaca atgttattac cttgatgagg tagtgtcaat attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 atgaaggtcg gtggggcttt gaggcggctg cgttcttgaa tgagggcggc gaagccttcg
      841 acgctgagcc tgccgctgcc gacataaacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaaa tggttttgac gggggatttt gcttggcact cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccaatcaa ataggcggca gcctgccgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac ggtggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgttggg ggggggggat gcagttgcta
     1621 cgaatcgcta tcctgtgaat tcaccctgtc aggagtgccc gaatcgtcat tcccgcgcag
     1681 gcgggaatct aggacgtaaa atctaaagaa accattttat agtggattaa caaaaaccag
     1741 tacagcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcatcaag
     1801 tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     1861 taatccacta tatccgataa gtttccgcac cgacaaaact agattcccac tttcgtggga
     1921 atgacgggat gcaggttcgt gggaatgacg cgaacagaaa cctcaaatcc cgtcattccc
     1981 gcgcaggcgg gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtct
     2041 gggattctgg attcccactt tcgtgggaat gacgggatgt aggttcgtgg gaatgacgcg
     2101 gtgcaggttt tcttaacccc gcgttctaga ttcccacttt cgtaggaatg acggcggtag
     2161 atttggcaga tgcggcggat tcggcaggtc tcaacccatc ctacaatcca ccctgaccgc
     2221 cttttacgaa tccgccgcca tcctgggaaa cgcaaaaaaa tgccgtccga aaacctttca
     2281 gacggcattt tcgcgggcaa atcagtaaaa gacttcgcgc cagcttaagc gtttcatacc
     2341 gcacgtcgga ccgggcgggt ttcgggtttc ggaagagcgg tgtgaaatga aacgcggcag
     2401 acggcgcgtg tgccgccatc ctgccccgaa ccggcggcaa agcgtcatcg cggtttgaat
     2461 cgggcgggcg cgtgcctgtt ccggcacgga tgccgtctga aagcggcggc agcggtatgt
     2521 gtcgccggct gtgatgctgc tttccggatg tccgtttggg aggaggtctg aattggcgga
     2581 tgcctgcccg ggccgcgtca gcctaaggcg aaaagttccc tgccgtggat tttgagccac
     2641 tgtttcgctt cgggcgtgta gggtgcgaag cggcgggtca gtgcccagaa cgcgcggctg
     2701 tggtcggcgt gggcgaggtg gcagagttcg tgtatgcaga catagtcggc aacgtattcc
     2761 ggtgcgccga ccagccgcca gttgaagcgt atgcctgtgg ttttgcggca cacgccccag
     2821 aaggttttgg cagaggtcag cgaggagggg gcggggaaca gttgtgtggt gcgggcgtgg
     2881 cgttcgaggc ggggaatcag gtaactgtgc gcctgccgtt ccaaaaagtc ccgcagcagc
     2941 gcaagctgtt tttcgggtgc gccttcggga acacggattt cagacggcat cagcaggatt
     3001 tgcgtgtctt gatgggcggt gagggcaagc tgtctgccgt ggaagaggat ggattcgggc
     3061 agccggtttt cggcagtttg cggcggcggt gttttcgcca gtgtttgccg caggacggct
     3121 tcgttttcat acagccagcg gtttagagcg gagacggaga agcagggtgg gacgctgatg
     3181 cggacggtat gtgtgccggc ggggcggata atcaggtttt tcttggcacg gcgcttgatt
     3241 tcgacggtca gttccatgcc gtctgaaagg gtgtggacaa aggcggtcat gcggtttcag
     3301 acggcatttt ggcggcaaac gggcctgcgc cggaaatgag cggctgctgc gtttcgatga
     3361 ggtgttcgca ttttcccatc aattcggctt cgctgccgct tgcgtgcggg atggtcggac
     3421 agatgacgac ggtgatttcc cccggatatt tcagaaagga gtttttcggc caaaattcgc
     3481 cgctgttgag ggcgacgggg acgatgtcca tctcaaacat tttcgccatg cgcgcgccgc
     3541 cgagtttgta tttgccgcgt tttccgggcg caaggcgtgt gccttcgggg aaaatggtaa
     3601 tccaatagcc ttcgtttttg cgcgccaacc cctgttttat gagctgctcg ttggcttcgc
     3661 ggcggttgtt gcggtctatg cctatggttt tgaccagttt caagccccag ccgaaaaagg
     3721 ggattttgaa caactcgcgt ttggcaacgt aaacctgcgg cggaaaaatg tcctgaaggg
     3781 cgagcgtttc ccagccgctt tggtgtttgg cgcagatgac ggcggggcgg tcggggatgt
     3841 tttccgcgcc gatgatgcgg tatttgagcc cgacgatgtg tttgagcgag aggttgagga
     3901 ttttgaccca gacccgcgcc atcttgtgcg ccccgtctcg gaaaggcgag gcgagcagca
     3961 taaagggaaa gaggaaaatc agggtggaac agagtatcag ccagtaaatc aggttgcgga
     4021 tgatgagcat ggttttgcct tgtcggaatg cggtatgttc agtcggcttg cggtgcggcg
     4081 ttttcctgca tgatgtattg tgagaaatcg agcaaggtat cgaaaacctg tgtgtgttcg
     4141 ggtaattcgt gtccgtgttg ggagagcgtt tttttgcctt ttccggtcag aaccagcgcg
     4201 ggttttccgc cgacggcatc gattgcctgc aaatcgcgca ggctgtcgcc gaccagccag
     4261 gtttccgagg cttgggcgtt gaagcgtccg atgatgtctt caatcatacc cggtttgggc
     4321 ttgcggcagt tgcagttgtc ggcatcggtg tgcgggcaga accagatgcc gttgatttcg
     4381 ccgcctgcct gacggacgag gcggtgcatt ttggcgtgca tttcggtaag gttttgaacg
     4441 gtaaaatatt tgcgcccgat gccggattgg ttggtggcaa cggcgacggt gtagcctgcc
     4501 tgcgtcagaa atgccaccgc atccatgctg ccttcgacag gtatccactc gtcaacggat
     4561 ttgacgaagt catcgcggtc ctgattgatg acgccgtcgc ggtcgagaat gatgagtttc
     4621 atcgcgtttc ctttgggttg gggcgtgtcg gggatgggat tataggacat aaatctagat
     4681 taaattctgc tacaagatgt ttctctaact ccaatatttt atcttgtgat ctgcgcttca
     4741 gtggtttaca atctaccccg acataaatag tccacggctc aagatttttt ctcactatag
     4801 cccctgctcc aacagcaacc ccatcaccaa tagtaacgcc tggtaatact gttacatttg
     4861 ttcctaaaat tgcatgtttt ccaattctaa cactagattt tttaacatta gtatatttag
     4921 atgggactgt tggatt
//
LOCUS       DAWFKN010000118         4899 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFKN010000118 DAWFKN010000000
VERSION     DAWFKN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.118"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(458..838)
                     /locus_tag="WGR46_002021"
     CDS             complement(458..838)
                     /locus_tag="WGR46_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0856877.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1310..3391
                     /locus_tag="WGR46_002022"
     CDS             1310..3391
                     /locus_tag="WGR46_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0856878.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3381..3575
                     /locus_tag="WGR46_002023"
     CDS             3381..3575
                     /locus_tag="WGR46_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0856879.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(3771..3962)
                     /locus_tag="WGR46_002024"
     CDS             complement(3771..3962)
                     /locus_tag="WGR46_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856880.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            4091..4651
                     /locus_tag="WGR46_002025"
     CDS             4091..4651
                     /locus_tag="WGR46_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0856881.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1168 a         1386 c         1206 g         1139 t
ORIGIN      
        1 gcgatgtgtt tgaacctgtt gaaagcggcc aacaggttaa gtgcgcccgc tgccgcctaa
       61 aaagcagccc ggatgcctga ttatcgggta tccgggcagg attaaggggg tgtttgggta
      121 aaattaggcg gtatttgggg cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg
      181 ggagggaaag gaattttgca aaggtctcag gccgtctgaa aaacagcttc gacaaagacg
      241 cggttgccaa agagatcgac ctacaaaggg aagtaacaca ggcgttcggc aaaacgtcgc
      301 ccaagtcgca gcggccgttg ccaacaaact tggcaatacc cgaagttacg gacggtatca
      361 ggtagccaaa accctgccgg aggccgaact gcaaaacaaa tccgacataa aaaaccgccc
      421 gaaaaatccg gacggcggtt caaacaggct gccccgttta gcgggcgcgg caggaggttt
      481 cgaccgaatt gccgtaggca tcggtaaagc cgaaaaaggc ttcgccgcct ttctgatgcc
      541 actcggttcc gtttccgaac aaaccgtgtt cggcggtata gcgttcgccg gatgcggcaa
      601 cgtcggaaga gaggacggca cgcctgccgt ccaaccgcaa cgcgattctg ccgccgtcca
      661 aacggcggac gtgcacagac aaaccgttct cgcaggaaaa tgcccgaaaa tctgccgtgc
      721 cggcttggtt ttgaacgggc ggcatatgcc ctcgtccgcc gtcatcatac gcctccggca
      781 cggcacaggc cgccaaagac aaaaccggta cggtcagcgc gaaaaacctg atattcataa
      841 aagctcccca ataaaaataa gatatgaaac aaccgccctg attccaagct gcggcaacgc
      901 catactataa acggacgcgc aaacacacaa gcccgataac cggaatttac ccgcgatgaa
      961 tcaataatcc ggattgcgcg cccctttccc gtattccggc accgcctttt gcctgacgat
     1021 gccgtctgaa cctcccttcc cgtcaggagg aatgtaaatt ttttccaaat tccaagtaaa
     1081 aaccgctatc agtgtgctaa tttgcgttaa aatcctattc ggcgtttaac gttttgtgcg
     1141 cccgcatccc tgcactgttt gatgcgggca taaggcacaa atcccgacaa gcgcactgtt
     1201 tcatacttcg tcaatcattc agactccggt ttgtgcccgt gccggcaggc ggtcatccgt
     1261 ttcccgccgt tcaggcatat tccgacagtg tgagataagg atttattcga tgaaatcact
     1321 caaaaccttc ctcatttggg gcatagtggt actggtcggc ttagcatcct ttaccactct
     1381 ggctctcagc cgaggcgaac aggtcagcgc ggtatggatg gttaccgccg ccatatccgt
     1441 ttactgcatc gcctaccgtt tttacagcct ctacatcgcc aaccgcgtaa tgcggctcga
     1501 tcctaaccgc ctgactccgg cagaacgcca caacgacggc ttggactacg ttccgacgca
     1561 caagggcgta ttgttcggac accactttgc cgcgattgcc ggcgcgggcc ctttggtcgg
     1621 ccctgtactg gctgcgcaaa tgggttacct gcccggtact ttgtggatta tcttcggcgt
     1681 ggtttttgcc ggcgcggtac aggatatgat ggtactgttt gtttctatgc gccgtgacgg
     1741 taagtctttg ggcgatattg tgaaacagga actcggcact gtccccggcg tgattgcctc
     1801 catcggtatt ttgatgatta tggtcatcat tatggcggtg ttggcgttga ttgtcgtaaa
     1861 agcacttgtt cacagccctt ggggtacgtt taccatcgcc gccactatgc cgattgccct
     1921 gtttatgggt atttacacgc gctatatccg tccgggcaaa atcggcgaga tttccatcgt
     1981 cggctttatt ttgctgatgc tggcggtaat ttacggcgaa gatgtggcga aaagttccat
     2041 cggacattgg ttcgaccttg acggcatcca gctcacttgg gcgattatga tttacggctt
     2101 tgtcgcctcc gtattgcccg tatggttgct gctcactccg cgcgactatc tctccacctt
     2161 cctgaaaatc ggtacgattg cggccttggc tttgggcatc gtcatcgtca atcccacttt
     2221 gcaaatgcct gccgtaaccc actttatcga cggttcgggt ccggtattct caggcgcatt
     2281 gttcccgttc ctcttcatca ccatcgcctg cggtgcggtt tcgggcttcc acgcgctgat
     2341 ttcctccggc accacgccga aaatgctgga aaacgaaacc cacgtccgta tgattggtta
     2401 cggcggtatg ttgatggaaa gtttcgtagc cattatggcg cttgccgccg ccgcatcgct
     2461 tgatcccggc gtgtacttcg ccatgaacag ccctgccgcc ctgatcggta cggatgccaa
     2521 caccgccgcc gaagtgatta ccaccaagct gcaattccct gtcgatgcgg caaccctgtt
     2581 gcataccgct aaggaagtgg gcgaaaacac catcctttcc cgtgcaggcg gtgcgccaac
     2641 cctcgcagtc ggtatggcgc acattatgag ccgcctgatt ccgggcgagg cgatgatggc
     2701 gttctggtat cacttcgccc tattgtttga agccttgttc atcctgaccg ccgtcgatgc
     2761 cggtacgcgc gtcgcacgtt ttatgattca agacttgggc agcatcttct acaaaccttt
     2821 cggcaacacc gactccatcc ccgccaacct gattgcgacc ttcttcgccg tggcattgtg
     2881 gggctacttc ctctacaccg gcgtaaccga cccgctgggc ggcatcaact cgctctggcc
     2941 tttgttcggc atcgccaacc aaatgctggc aggcgtggcc ttgattatgt gcgccgtggt
     3001 gctgattaag atgaaacgcg accgttatgt ttgggtggta ctcgttcccg ccgtcggcgt
     3061 actgttcgta acctgctacg ccggcctgca aaaactgttc cacagcgacc cgcgcatcag
     3121 cttccttgcc cacgccggca aatacagcga cgcattggct aaaaacgaag tccttgcgcc
     3181 tgccaaagac atcggcgaaa tggcgcaaat catcttcaac gacaagatta atgccggtct
     3241 gaccatcctc ttcttgtcgg ttgtcgtgat tgtcgccgcg tacggtttgc gtaccgccct
     3301 caaagcacgc aaagtcggct ggccgaccgc caaagaaatc ccggcggtgt accgcgacgg
     3361 caagcagccg gaggcacaaa gtgaagcata agctcgcgtc ttggtggaaa accatcaagc
     3421 tgacggcaaa cttgatggca ggcgtgcctg attatgaaaa ctacgttgca cagcagcgca
     3481 aacataatcc caacgccccc gtgatgacca agctgcagtt tcaagactat tgccgcaaac
     3541 gccgctgcgg cgcaaacggc ggacgctgct gttagccccg cctgaaacaa atgccgtctg
     3601 aacgccgctt cagacggaat ttttataata tagtggatta acaaaaatca ggacaaggcg
     3661 acgaagccac agacagtaca aatagtacga aaccgattca cttggtgctt cagcacctta
     3721 gagaatcgtt ctctttgagc taaggcgagg caacgacgta ccggtttttg ttaatccgct
     3781 ataccacgat gaatccttcg caatatctgt ttatcgacct caattttgac aaaataccgg
     3841 atacgcgcct ttgttgcttt tccatcttcc aaccaactgt aaatctcaaa cagccggtac
     3901 acgccatgct tcagtttctt ttcctgtcgg cggattgttt cgacaaagaa ttgaaaatcc
     3961 atttcatgca ccttaaaatt taatctgcat tcaaaccttt tcactttgga agcaccattt
     4021 atcggatgtc ccttcgcaat aaacaaattt tcccgatacc gccgcccatt tcaacccaaa
     4081 cccaaaacct atgaaaaacc tcatcgcctt caacaaaccc tatggcgtta tctgccaatt
     4141 ttcaccgcac gaaaaacaca aaagcctcaa agactttatc aatcttcccg gcttctaccc
     4201 cgccggacgg ctcgacaccg acagcgaggg gctgctgctg ctgaccgacg acggcaggct
     4261 tcaggcacaa attaccgacc ccaaattcaa acaccctaaa acctactggg cgcaactgga
     4321 gggcgtaccc gacgaaagcc gattggaaag cctaagaaaa gggatagact taggcggttt
     4381 cgttacccgt ccggcaagca tccgcatctt gaaacacgga gaagcagatt cgttatggga
     4441 gcgcatcccg ccgatacgcg tccgcaaaac cgttcccgat ttttggattg aaattaccat
     4501 ttctgagggc aaaaaccgcc aagtcaggcg aatgaccgcc aaggcgggct atccctgcct
     4561 gcgtctgatc agagtggcaa gcggcaggct gaaactgttt gatttggatt taaaacccgg
     4621 ggaatgggca tacgccccgt ttaaaccata atcacgttta tctcatcatt tccacaaaag
     4681 tgggaatccg gaattttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     4741 acagtacaaa tagtacgaaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     4801 ctttgagcca aggcgagaca acgccgtact ggtttttgtt aatccgctat attccgccat
     4861 ctctaagatt tacagcgata cacgggtgat ttaaggaat
//
LOCUS       DAWFKN010000119         4769 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFKN010000119 DAWFKN010000000
VERSION     DAWFKN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.119"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..184
                     /locus_tag="WGR46_002026"
     CDS             <1..184
                     /locus_tag="WGR46_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856882.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYS
                     GLTKIRTRRRSRRQYR"
     gene            282..398
                     /locus_tag="WGR46_002027"
                     /pseudo
     CDS             282..398
                     /locus_tag="WGR46_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGR46_002028"
     CDS             complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGR46_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0856883.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1537..1719)
                     /locus_tag="WGR46_002029"
     CDS             complement(1537..1719)
                     /locus_tag="WGR46_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856884.1"
                     /translation="MPSERGSAFRRHFRRRQADFGRRKRQRAAFCKAADCGGYRISRR
                     ALAPLSHLPYLFGTYL"
     gene            complement(1741..3168)
                     /locus_tag="WGR46_002030"
     CDS             complement(1741..3168)
                     /locus_tag="WGR46_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0856885.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3353..>4769
                     /locus_tag="WGR46_002031"
     CDS             3353..>4769
                     /locus_tag="WGR46_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0856886.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAA"
BASE COUNT         1023 a         1332 c         1299 g         1115 t
ORIGIN      
        1 cgacaagttg gacgtgagcg gttttatcca ttaccgcatc aaccattcca aggagtttgc
       61 agaccgtcag aaccacatta acggcattga gaacttttgg aatcaggcaa aacgcgtctt
      121 gcgaaaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag
      181 atagtacgga accgatttac ttggtgcttc agcaccttag agaatcgttc tctttgagct
      241 aaggcgaggc aacgctgtac tggtttaaat ttaatccact atacaacgga atcgattgca
      301 aatctttccc actgttcttg aaagaatgcg aatttcgatt taacttcggc acaccgtctc
      361 aacagcttaa aatcctgcgg gattggtgtg ggatttaggg ctaatctagt acagcccctt
      421 ttaaaaaaca ttacaatccc gggccgccaa gcgaattggc aatgtccctg accgccgtta
      481 catacatccg gctgtggttg tactgccata ccgtataaaa attgttcaag cccaaataat
      541 attcaaacac gccgggtgcg gtttccagtt tgaacaaaac cgccttttca tcatcggcaa
      601 gctcttcgcc ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc gtcagggcgg
      661 ttttttcgcc aatgattgcc tgaacatccg cacccggcgc caatgttgca gacaccagta
      721 ttttcccgcc cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat gccgcgacat
      781 cgccgatgtt gccccatatg tcccgatgtc cgtccccgtc ataatccacc gcccatttcc
      841 ggtagctcga aggcataaat tgcggcatcc ccattgcgcc cgcatagctg cctttaaagg
      901 cgaaaacatc gccgccttct tcttttgcca gctttaaaag ctcgaccaat tctttttgga
      961 aaaacccggc gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc gccacacgga
     1021 aactgcccgt atttttgccg taattcgttt caatcccgat aaccgccacg ataagttcgg
     1081 caggcacgcc gtatttttgc gccacatcat cgataagcgc gcggttttcc gcataaaacc
     1141 ggcgcgcgcc gcgaaatttc gccttgcccg aatttcccgt gcggaacaca taccacggac
     1201 gcgatgtgga ggggcggtgc ataatcttga cgatgtccgc cttgtaagcc gctttgtcaa
     1261 aaaaatcctg ccattccgcc cgggaaaaat cccctttccc gacttcatcg tccacaaaac
     1321 ggcggacatt tgcattggcg gcaaacccgc tgtcggatac cggtacggct gccgcgtcaa
     1381 atacggctgc cgcgtcaaac gcggggcggc tttctttttt catttcatcc gcgcgggggg
     1441 cttgggcttc atttgcccgg ggcgggcgtg cctccatcgc cgtacaggca gacaaagccg
     1501 ccaaacaaat tgccagcggc agtatttttc tctttttcat aaatatgttc cgaacaaata
     1561 gggtaagtgg gaaagcggcg caagggcgcg gcgcgaaatg cggtatccgc cgcaatcggc
     1621 ggctttgcag aatgccgcgc gttgcctttt gcgccgcccg aaatctgcct gccgccgccg
     1681 aaaatgccgt ctaaaggcac ttcccctttc agacggcatt gcctgcgccg tatttgcccg
     1741 ctatccgcaa tatcggcagt ccaatatgtc tttgcggata tcgttcagca ggcaggctgc
     1801 cgcgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgataaa
     1861 gactttttgc agggcgcagg cgtcggtcag cgtgtgttcc tgcaggaagc tgcggatgtc
     1921 ttcgggtaag gtgtaatcac gggcaacggc ggcaaagcgg gcatcggttt gcaggcggtc
     1981 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
     2041 gacaaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
     2101 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
     2161 caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
     2221 gatgttcgcc tccatcagct gatgggcgac gggggcaaca ggcgaaacgg aatggcagac
     2281 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
     2341 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaaggatgt attccataat
     2401 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
     2461 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
     2521 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
     2581 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
     2641 ttctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt attttgcggc
     2701 gttgacgtaa atggtttgtc cgtcggtata ttcgtagtaa gactgcattt ctcgggcaat
     2761 cgccgcgccg gaggtttcgg gttcggtaac gcccaaacca ccgccctcgc ctttgaaaac
     2821 catgtccaaa ccctgtgcga tttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
     2881 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
     2941 caagacttcc tgaatttcaa actggctgcc cttgcgcccg ccgtgttttt tgtcgaggaa
     3001 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
     3061 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
     3121 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
     3181 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
     3241 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagacg gcatcgcgcc
     3301 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
     3361 ccccatcccc aaaccccgtg aaaaatcccg ttggtccaat ctttcgcaag gctcgctgcc
     3421 cttggctttg gcgcgttatc tgccgcataa gcggctcaag gtcgtgctga cccaagatgc
     3481 ggaacaggcg ctacgccttc agacggcatg gcggtttttc cgtccgcacg acacggcggt
     3541 gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tcgccgcatc aggatttggt
     3601 gtcggaacgg ctgtcggcgt tgtggcagat taagagcggc gcggcggacg tgttgttcgt
     3661 gccggttgcc acggcgatgc agaagctgcc gcccgtgccg tttctggcag ggcgcacgtt
     3721 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaaccgatt tggtagatgc
     3781 gggctacaac catgtttccc atgttgttgc ggcgggcgag tttgccgtgc gcggcggcat
     3841 tgtcgatttg ttccccatgg gcagcgaaat gccctaccgc atcgatttgt ttgacgatga
     3901 aatcgacagc atcaaaacct ttgatacgga cacgcagcgc accatctccc ccgtttccga
     3961 aatccgcctg ctgccggcgc acgaattccc caccgacagc gaggcgcaaa aaatcttccg
     4021 cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt acaaagccgt
     4081 cagcaacggc cacttcggtg cgggcgtgga atattacctg ccgctgtttt ttgaaaacga
     4141 gctggaaacg ctgtttgact atatcggcga ggatgcgctg tttgtctctt tgggcgatgt
     4201 tcacgccgag gcaaaccgct tttggagcga cgtcaaatcg cgttacgcca tggcgcaggg
     4261 cgatgaaact tatccgcctt tgcttccaca gcatttgtat ctctctgccg atgtattcgc
     4321 aggccgtctg aaaaactacg gacaagtgct gcccgatgtt tccggcaagg aacacatcct
     4381 gcccgacctt gccgtcaacc gccaatccga cgagccgttg caggcattga aggattttca
     4441 gacgaccttt gacgggcgga ttttgctgtg cgccgaaagc ttgggacggc gcgaaaccat
     4501 gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact ggcagggctt
     4561 cttgtcggcg catgagccgc tgatgattac agtggcgccg ttggcttacg ggtttaaact
     4621 gggcggactg caatcgccga accaacagca aactacttcc gcctccgaag gagaaggcga
     4681 tgcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc ctagccctct
     4741 cccacaggag agggaacaaa gtgcagccg
//
LOCUS       DAWFKN010000120         4730 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFKN010000120 DAWFKN010000000
VERSION     DAWFKN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.120"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(432..968)
                     /locus_tag="WGR46_002032"
     CDS             complement(432..968)
                     /locus_tag="WGR46_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0856887.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1002..1358)
                     /gene="folB"
                     /locus_tag="WGR46_002033"
     CDS             complement(1002..1358)
                     /gene="folB"
                     /locus_tag="WGR46_002033"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0856888.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1414..2016
                     /gene="plsY"
                     /locus_tag="WGR46_002034"
     CDS             1414..2016
                     /gene="plsY"
                     /locus_tag="WGR46_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0856889.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPTTALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            2104..2949
                     /locus_tag="WGR46_002035"
     CDS             2104..2949
                     /locus_tag="WGR46_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0856890.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3195..4169
                     /locus_tag="WGR46_002036"
     CDS             3195..4169
                     /locus_tag="WGR46_002036"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0856891.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4328..4546)
                     /locus_tag="WGR46_002037"
     CDS             complement(4328..4546)
                     /locus_tag="WGR46_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0856892.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1218 a         1396 c         1006 g         1110 t
ORIGIN      
        1 gttctcaaat tcaaattggg cttttaggtc tataacaaag aaaaagcctt ggtaggataa
       61 acctaccaag gctttttctt aaaaaaacgc tctattaccg tgcctaaaaa tgcaaaaaag
      121 taaccgtcat aacccttttg ccaaaatcaa tatcatcatc cgatgaatga attgacggta
      181 tataaggacg gtatataaga aagaattaat aagctcataa accggtaaga aaaatcagta
      241 acaaagatat tcattattaa ccattttaaa aattatcatg ttcgtttggg taattattat
      301 acccttttcc ctcttattac taaagcacga atgactttaa aggacgcgcc cagagacaag
      361 ccgaaaaggc atatctcatc cgccaaacaa aatgcccacc gacttactcc ggcaggcagt
      421 tcaacaccct gtcaatcctt catcagccag tattgcaaac cgattaatgt cttgccgtct
      481 ttaatttcat cgtttgccaa tgcctgacgg acttcttctt tcgacatcaa tacggtttcc
      541 gtaatctcgt cttcgtcatt ggcaagcgta ctgcccaaac gcacgccttc cgcctcgaac
      601 agatacattt tttcgttgca aaaaccgacc gccgtataaa aactgtacag caggcgcacg
      661 ctgtcggcaa cataaggtgt ttcctccgcc aattctcgca gcgcacacgc tgccatatcc
      721 tcgcctgcca catccagctt gcccgcagga agttccaagg tcgcctgatt tgccgcataa
      781 cgccactgcc gcaccaaaac gaccttttca tcatccgtta ccgccaacac gcacgccgcg
      841 cccggatgac ggatgacgat acgctgccct tcattgccgt tgggcaacct gaccttatcc
      901 ctgctgatgc tgacaaaacc gccctcgtaa atggtttcgc cgcctaattt tacttccctc
      961 aaatccactt catatctcct ttccgtttcc tattctgccg ttcaatcttc acgcttgccg
     1021 cgctcgattt ccacgccaac ctcgcgcacg ccttccaaaa tacccggctt gacgattttc
     1081 acgcgtaccc acaccgcgcc gaaatatccc aaaaccaaat cggcaatata ttccgccaac
     1141 gcttccaaaa gcaggaaatc ctgttctttc agatgtcggc gcagcgtttc gcatacctcg
     1201 gcataatgca ccgtattggc aatatcgtcg tccgaacccg ctttctcggg aacgccgatg
     1261 tccaaatcga caatcagggt ctgcaaccgt tcgcgctccc agccgtacac gccgataagc
     1321 gtatccgcct tgatgccgtg caaaaagatt ttatccataa tccgccccgt tttttgctaa
     1381 aataaccgca ccattctaag taaagtaccg caaatgttca acataccggc tgttgccgtt
     1441 tcctatctga tcggctcact ttctttcgcc gtcatcgtgt ccaaatatta cggcatggac
     1501 gacccgcgca cctacggatc gggcaatccg ggggcaacca atgttttacg cagcggcaaa
     1561 aaaaaggcgg ccgcgctgac gctcttgggc gatgccgcca aaggtttggt tgccgttttg
     1621 cttgcacgcg tgcttcaaga accgctcggt ttatccgaca gcgcaatcgc ggccgtcgca
     1681 ctcgccgcgc tggtcgggca tatgtggccg gtgtttttcg gatttaaagg cggcaaaggc
     1741 gtggcaacgg cattgggcgt gcttctggca ctctctccca caactgcctt ggtctgcgcg
     1801 ttgatttggc ttgtgatggc attcggcttc aaggtgtcct cccttgccgc attaaccgcc
     1861 acaatcgccg ccccccttgc cgcactgttt tttatgccgc atacttcttg gattttcgca
     1921 accctcgcaa tcgccatatt ggtgttgctc cgccataaga gcaacatcct caacctgatt
     1981 aaaggcaaag aaagcaaaat cggcgaaaaa cgctgattcg gcatttgttt tccattacgt
     2041 catgccgtct gaaaccatgt tcccgcgttc agacagcatt tccgcacaaa cggctttcat
     2101 accatgctca gttacagaca cgcattccac gccggcaacc acgccgatat gctcaaacac
     2161 tttaccttgt ttttagtgct gcaatatttc aaccgcaaag acaagcccta ctggtacatc
     2221 gacacgcacg gcggcgcggg tgtgtacaat ctcgaaggca gcgaggcgca gaaagtcggc
     2281 gaataccggc aaggcatcgc cctgctccga caggcgcaaa acctgcctgc cgaactctct
     2341 gactttgccg cgcacataca aaaaatcctg ccctcgcccg aactttactg cggttctccg
     2401 tggctggcac aatcgctgac ccgcgtcggc gacaaattac gcctgtttga gctgcacccc
     2461 accgactttg tccatctgca aaacaatatg ggcgaagcag gattgggcaa acgcggacaa
     2521 gtgttgcgcg aagacggtta caaagggctg atttccctgc tgccgccccc gccgcgccgt
     2581 gccgccgtcc tcatcgaccc gccttacgag gaaaaacagg actactggcg tgtaacggaa
     2641 acgctgaaat ccgctttaaa acggtttgag tccggctgtt atctcatctg gtatccctgc
     2701 ctcagccgcg aagaaagccg caagctgccc gaagaactga aaaaactcgt tcccgacaac
     2761 taccttcacg ccgaactgca cgtccacgcg ccgaaagccg acggtttcgg tatgcacggc
     2821 agcggaatgt tcgtcatcaa tccgccctat ctgttggcgg aacaattggc ggcaaacctc
     2881 cccgccctga cgcggctgct ggcgcaagac gaaggcgcgc gctacctttt ggacagcaaa
     2941 atccgctgac cccgatgccg tctgaaaccc ttcagacaac atctcccgtc aaataaacaa
     3001 aaccggacga tataccggca gtcggcactt ttaagaataa atatcccctc atccgcctgc
     3061 cgcgttccga cttgccccat gccgtttttt tacactacaa tcccctagac tatcctgcaa
     3121 aacgcaccaa aggaatacgc cacaataacc tgccgccttt cagacggcac aaacctttcc
     3181 gaaacgagca aactatggac acactgaccc gattcctccc cgaacacctg caacaaaacc
     3241 agctgcccga agcactcggc ggcgttttac tgtccgttgt ttccgcctgc accgaaatca
     3301 acgccaaagt ccgcctcggc gcactggccg gcgtattggg tatggcgggc acgggcaata
     3361 ttcagggcga agaccaaaaa aaactggatg ttatcgccaa caacatcatg attgacacac
     3421 tcaaagccaa ccctgccgtt gccggtttgg caagcgagga agaagactct ttcgtaagtg
     3481 ccggtgaaaa cggacgctat ctcgtcctat tcgatccttt agacggatcg tccaatattg
     3541 atgtcaacat ttccgtcggt acgattttct ccatccttga aaaacccgaa ggcgcattgg
     3601 caaccgaatc attccttcaa acgggcagac agcagcttgc cgctggttac gttctttacg
     3661 ggcctcaaac ccagctcgta ttcacattcg gacacggcgt atatatgttc acgctcaatg
     3721 ccgaaaacga atttgtgctg acaaaagaaa aaccgaaagt acccgaaagt accaaagaat
     3781 ttgccatcaa tatgtccaac cgccgccact ggctgccccc cgtccaacaa tacatcgacg
     3841 agctcttggc gggcgaaacc ggcacgcgcg gcaaaaacta caatatgcgc tgggtagcca
     3901 gcatggttgc cgaaatccac cgcatactga tgcgcggggg cgtgttcatg tatccgcaag
     3961 acaaacgcga cccgtccaaa cccggcaaac tgcgcctgat gtatgaagcc aatcctatgg
     4021 ccctgatact cgaacaagcc ggcgcatcgg caagcaacgc ccgtcaggac atactcggca
     4081 tccgtcccga aagcctgcac cagcgcgtcg ccgtcattat gggcagcagc gaagaagtgg
     4141 attacctcaa ccgcctgcat tcaaaataaa aggcagcctg accgataaag aaaggcggca
     4201 gcgcgcatcc gacgctgccg ccgatatttc gtttatagcc ttaaaaagat agaaaaccac
     4261 ctttccgaac ggcaaaaatg ccgtctgaaa agttttcaga cggcatttta aaaattaaag
     4321 cccgaattta tttgcccgct gctttttgca ggatggcgta caactcgtct ttcagtttca
     4381 atttggcttt tttcagctca tcgatggttt ccgcaccgct ggtaaccgga ttgttgacca
     4441 gaccggtaat tttatcgtcc agctcgttgt gttcgtcgaa cagacgggcg aagcgagaat
     4501 tttcctgttt caatttggaa atcaaatcac ggtattctgg gaacatatcg ctttcctctc
     4561 ttggtttacg gttaaaaagt caaattgaat ctttacatta attataacaa agataatatt
     4621 tttatccagt gttttatcgg atacggcaaa attttctttt aacaccgccg tccggttgat
     4681 aaaaacgttg acaatatcac gctttacgca taaattttca gcctttccta
//
LOCUS       DAWFKN010000121         4563 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFKN010000121 DAWFKN010000000
VERSION     DAWFKN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.121"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            22..256
                     /locus_tag="WGR46_002038"
                     /pseudo
     CDS             22..256
                     /locus_tag="WGR46_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            424..2199
                     /gene="ggt"
                     /locus_tag="WGR46_002039"
     CDS             424..2199
                     /gene="ggt"
                     /locus_tag="WGR46_002039"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0856893.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2418..2613
                     /locus_tag="WGR46_002040"
                     /pseudo
     CDS             2418..2613
                     /locus_tag="WGR46_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2680..3930
                     /locus_tag="WGR46_002041"
     CDS             2680..3930
                     /locus_tag="WGR46_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0856894.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4058..4563
                     /locus_tag="WGR46_002042"
                     /pseudo
     CDS             4058..4563
                     /locus_tag="WGR46_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1256 a         1130 c         1058 g         1119 t
ORIGIN      
        1 aggcacgccc cagtttcccc aatgtggatt ttcagcgcgt gcagtagagg tgctgaccca
       61 attggcagac cctttgcttt tgttaatatc ctagaaaacc cagaaattcg cagtactcta
      121 ccactcatcg ccaatcggcc aacctttcca cagctttggg ttaatggtga gttaatcggt
      181 ggtagtgata ttatcacaaa tgtaccaatc aggtgagcta aaaccactca ttgaagagca
      241 tagcccaaag gtctgattac ttaatatctt aaaataaata tcaaaaaaga ctgctgatga
      301 cattagcggt ctttttttga tggtcaagat tgatagtttg gattgaatta tttagtcata
      361 ttaaatacaa taatcagcat gccttgtatg aatcatcaat caaactcagg cgaaggagtg
      421 cttgtggcta aaacatattt attgactgca ttgataatgt ctatgacaat ctctggatgt
      481 caagtcatcc atgccaatca aggtaaggtt aatactcatt ctgctgtcat cacaggtgca
      541 gacgctcaca cgcctgaaca tgcaacggga ctgaccgaac aaaagcaggt gattgcaagt
      601 gattttatgg tagcgtcagc caatccatta gcaacacaag ctggctatga tatcttaaag
      661 caaggcggta gcgctgcaga tgcgatggtg gcggtgcaga cgacactaag cttggtagag
      721 ccacagtcgt caggcttggg cggtggtgca tttgtgttgt attgggataa taccgccaaa
      781 acattgacca catttgatgg gcgtgagacg gcaccgatgc gtgcgacgcc ggaattattt
      841 ttggataaag atggtcaacc attgaaattt atggaagcgg tggtcggtgg tcgctcggtg
      901 ggtacgcctg ctatccctaa actgatggag acaatacatc agcgatacgg tgtattgcct
      961 tggggaaaat tatttgatac accgatccgc ttggcaaaac aaggctttga ggtgtcgcca
     1021 aggcttgcca tctcggttga gcaaaatcag cagcatttgg cacgctatcc aaaaacagcc
     1081 gcttattttt tgccgaatgg tgtgccgcta caagcaggca gcttgctgaa aaatttagaa
     1141 tttgctgaca gtgttcaggc gttagcagct caaggtgcaa aagctctgca tactggtaaa
     1201 tatgcccaaa atatcgtttc agttgtccaa aatgctaagg ataaccccgg tcaattatcc
     1261 ttacaagatt tatctgatta tcaagtggtg gaacgcccgc ctgtttgtgt gacttatcgt
     1321 atttatgaag tatgcggtat gggtgcacca agctcaggag ggattgctgt gggtcagatt
     1381 ttggggattt taaatgaatt ttcacccaat caggtggggt atgatgctga aggtttacgt
     1441 ctcttgggcg acgcttctag gcttgcgttt gcggatcgtg atgtatattt gggcgaccct
     1501 gattttgtac cagtacccat tcgccagttg atttctaaag actacctaaa acatcgcagc
     1561 cagctgcttg agcagtcgga taaggcattg cctagcgtat ctgcaggcga ttttattcat
     1621 gagtgggtgt cctcacaagc gattgagtta ccttctacca gtcatatatc aattgtggat
     1681 aaggcgggca atgtgctatc aatgacgact tccattgaaa atgcgtttgg ctcaactttg
     1741 atggcaaatg gctacctgct gaataatgag ctgacagact ttagttttga gccaataaag
     1801 cagggcaaac aggtcgccaa tcgtgtcgag cccggcaaga gaccacgctc ctcaatggca
     1861 ccaaccatcg tatttaaagc aggcaagcct tatatggcaa tcggctcccc aggtggtagc
     1921 cgtatcattg gctatgtcgc taagacgatt gtggcacata gtgactggaa tatggatatc
     1981 caagatgcca tcagtgcacc caatttatta aatcgctttg gtagctatga actagaaacg
     2041 ggtacaaccg ccctccagtg gcaacaagca ttaaatgatt tgggctataa gactgacgtg
     2101 cgtgagctaa attcaggtgt tcaagccatt attatcgagc caagcaggct ggttggtggt
     2161 gccgatccac gccgagaagg tcgagtgatg ggtgattaga ggtgaagctt gtattttgtg
     2221 aatttgaccg cattaagtta atattaaaat agggggaaat tgtgagtaat caaacaaaaa
     2281 tacaagccat caagcaagct tctgagcaga tcttcgcaat ttgtgagacg ccaaacacag
     2341 cattacaagc cattcatctg attttaagac atggtggggc aggagagttg tcttggcaag
     2401 tggtgtatca gcgggttatg gctgataaag atgtggttgg tgctggttat ttgattgatt
     2461 ttgcacaaac tgccgaaaat ttaccatttg atgcattgcc tttgggtagc cttgtactaa
     2521 cataaaggta atgagacgct aaaaaccagc atgttaaata aattacctga taatgccaaa
     2581 gaaaatttac gcattatgga ctatctgcct taggttttga agtatttttt tatacaaaaa
     2641 acactcatga taccctgatt acacaaagga gccgagaaca tgttttcaaa aagcgttacc
     2701 ctcgcacaat atgaccccga tttggcagca gccattgccc aagaagacca acgccagcaa
     2761 gaccacgtcg agctgattgc ctctgaaaac tacgtcagct gcgccgtaat ggaagcgcaa
     2821 ggttcccagc tgaccaacaa atacgccgaa ggctaccccg gcaaacgcta ctacggcggc
     2881 tgcgaatacg tcgatattgt cgaacaattg gcgattgacc gcgtcaaaaa actgttcggc
     2941 gcgcaatacg ccaacgtcca accgcactcc ggctcgcaag ccaaccaagc cgtgtacgct
     3001 tccgttttaa aaccaggcga caccattttg ggtatgtctc tggcgcacgg cggccacctg
     3061 acccacggcg cgagcgttaa tatctcaggc aaactctaca acgctgttac ttatggtttg
     3121 gatgaaaacg aagtgctcga ttatgccgaa gtcgaacgcc tcgcgctcga acacaaaccc
     3181 aaaatgattg tggcgggtgc gtctgcctac gcgttgcaaa tcgactgggc aaaattccgc
     3241 gaaatcgccg ataaagtcgg cgcatacctc tttgtcgata tggcgcacta tgccggtctg
     3301 gttgccggcg gcgaatatcc caatcccgtg ccgttctgcg acttcgttac caccaccacc
     3361 cacaaaaccc tgcgcggtcc tcgcggcggc gtaattttgt gccgcgacaa tacccacgaa
     3421 aaagcgttga actcttccat cttcccaagc ctgcaaggtg gcccgctgat gcacgttatc
     3481 gccgccaaag ccgtggcgtt taaagaagca ttgcaacccg agttcaaaca atacgcaaaa
     3541 caagtgaaaa tcaatgcagc cgccatggcg gaagagttgg ttaaacgcgg tttgcgcatc
     3601 gtttccggcc gcaccgaaag ccacgttttc ctcgttgacc tgcaaccgat gaaaattacc
     3661 ggcaaagccg ccgaagctgc tttgggcaaa gcgcacatca ctgtcaacaa aaacgccatc
     3721 ccgaatgatc cggaaaaacc cttcgttacc tccggcatcc gcatcggttc cgccgccatg
     3781 accacacgcg gttttaacga agccgacgcg cgcgtattgg cgaacttggt tgccgacgta
     3841 ttgtctaacc ccgaagacga agccaacctc gccaaagtcc gcgaacaagt aactgctttg
     3901 tgcaataaat accccgttta cggcgcgtaa gcctttttaa aaatattccg ctaagcaatc
     3961 caatgccgcc tgaaatctca taatgtttca ggcggaaacc tttgcaaaat tccccaaaat
     4021 cccctaaatt cccaccaaga catttagggg attttccatg agcaccttct tccggcaaac
     4081 cgcgcaagcc atgattgcca aacatatcaa ccgtttcccg ctattgaagt tggaccaagt
     4141 gattgattgg cagccgatcg aaccatacct gaaccgtcaa agaacccgtt acctccgaga
     4201 ccaccgcggt cgtcccgcct atcccctgct gtccatgttc aaagccgttc tgctcggaca
     4261 atggcacagc ctctccgatc ccgaactcga acacagcctc atcacccgca tcgacttcaa
     4321 cctgttttga cgttttgacg aactgagcat ccccgattac agcaccttat gccgctaccg
     4381 caaccggcga cgcaagacga caccctgtcc gaattgccgg aactgatcaa ccgccaactg
     4441 accgaaaaaa acctaaaaat agagaaagca tccgccgccg tcgttgacgc caccattatt
     4501 cagaccgaga cctttgcaaa attcctttcc ctcccgacag ccgaaaccca aacacaggtt
     4561 ttc
//
LOCUS       DAWFKN010000122         4402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFKN010000122 DAWFKN010000000
VERSION     DAWFKN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.122"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(49..3093)
                     /locus_tag="WGR46_002043"
     CDS             complement(49..3093)
                     /locus_tag="WGR46_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0856895.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAGFEQAQHSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSNGQYLSETEAFGHDSRAVCPFEDVPSERPSCRASDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3258..3938
                     /locus_tag="WGR46_002044"
     CDS             3258..3938
                     /locus_tag="WGR46_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0856896.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3967..4326
                     /locus_tag="WGR46_002045"
     CDS             3967..4326
                     /locus_tag="WGR46_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0856897.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          889 a         1292 c         1129 g         1092 t
ORIGIN      
        1 aaatgccgtc tgaaacagtc ttcagacggc atttccattt gcaaaaaatc aagcattgtc
       61 caaggggacg agaatcgtac ggttgccgtt gtgttccggt gcggacacaa tgccttccgc
      121 ctccatctgg tcaatcagac gcgcggcgcg gttgtagccg atacgcaagg cgcgctgtac
      181 gcccgaaatg ctggctttgc gcgttttcag gacaacggat acggcctcgt cgtacatcgg
      241 atcggtttcg tcgtcgccgc tgcgcccgat gccgggcagc tcttcgctgc cgccgccgct
      301 caaaatatcg tcaacatagt ccggttcgcc aaactgcttc agatattcga ccacgcggtg
      361 cacctcttca tccgaggcaa acgcgccgtg aacgcgctgc ggataggccg tacccggcgg
      421 caggaacagc atatcgccct gaccgagcag gttttccgcg cccatttggt cgagaatcgt
      481 gcggctgtcg attttgctgg atacttgaaa cgcgatacgc gtcgggatgt ttgccttaat
      541 cagacccgtg atgacatcga cgctggggcg ttgcgtggca aggataagat ggatgcctgc
      601 cgcgcgggct ttttgggcga ggcgggcaat cagttcctcg attttcttgc ctgccgtcat
      661 catcaaatcg gcaaactcat cgaccacgac cacgataaac ggcagttttt ccaaaggttc
      721 gggatcgtcg ggcgtgaggc tgaacggatt gccgattttt tctccccttg ctgcggcttc
      781 ggcgattttt tgattgaagc ccgcaagatt acgcacgccc ataaagctca tcaggcggta
      841 gcgtttttcc atttcgttaa cacaccagtt cagcgcgttt gccgccagct tcatatcggt
      901 aacgacaggg gcgagcaggt gcgggatgcc ttcgtaaatg ctcaattcca gcattttcgg
      961 atcgatcata atcatacgca cgtcttccgg cgcggctttg aaaagcatag acagaatcat
     1021 cgcgttgaca cccaccgatt tgcccgaacc ggtcgtgccg gcaaccagca aatgcggtgc
     1081 tttgcccaag tcggttacaa cgggctgtcc ggtgatgtcc tgaccgagcg cgagcgtcag
     1141 cttggatttg gattcggcaa actcgggcga attgaagatt tcgctcaggc gtatcatttg
     1201 gcgtttcggg ttcggaagtt ccaaacccat acaggttttg ccggggatgg tttcgacaac
     1261 gcggatggaa gccacgccga gcgaacgcgc caaatctttt tccagattca gaacggaatt
     1321 gccgcgcacg ccgacatcgg gttcgatttc ataacgcgta attacggggc cggaataaga
     1381 atcgacaacc ttgaccttga ctttgaactc cgccaatttt tcttcgatgg tgatgctgtt
     1441 ttccagcagt tcttcttcgg tttgcgtcgc ctcgggattg aacagcggag gcagaagcag
     1501 gtcggttgtc ggcaggtgtt cggataccgc accggtttct tccgatggga acgccccttc
     1561 atccgcttcc gtatccgatg cccggcagga cgggcgttca gacggcacat cttcaaacgg
     1621 acaaaccgcc cgactgtcat gcccgaacgc ttcggtttcc gacagatatt gcccgtttga
     1681 ccgctctgcc gcaccttcac tgccgtcatc tgcaatagcg gcggtttcct cctgccaacc
     1741 tccattcaaa acatcatcgg caagatggtc ggtttcggca atgcggctgt gttgcgcctg
     1801 ctcgaatcct gccggcggtt cataggtacg gttgtagatt tccgatacgg gaggcggcgg
     1861 cggaatatcg attgcgggca tgggaacttt cggcacttcg ggtggttcga ccacccaagc
     1921 atcgggcgcg gcaggttctt caatatggat atcggcagtt tcatcataaa ccggaccgcc
     1981 gtatcccaca gacgaaacag tttccgtgaa aacattttcg gtttcagacg gcatttcgac
     2041 atcccgtttc ccgacaaccg tccgactttc cggaatcagg cgcgaaatcc gcgcctccgc
     2101 agaaaccgtt gccttgttcg gttctttcaa attgacggaa acgcggcggc gggcatcgcg
     2161 gacggctgtg ccgtctgaaa cggactgccc ttgggaaaca tccggggatt ttgcctccgc
     2221 ctgccctttc ccctgccctg catgacggcg gaacggcgca ttccgtgcat tttcggcttt
     2281 gactgcagaa aaggcagacg gatgctgctt ctcaaaatac ggctcaaatc cgtaatccgc
     2341 agactcggaa aacgcttctt tgtccgcatc gaacatacgg gaatacgtgc gttgaagcac
     2401 agggtcgtcc aaaccgataa tctgcagccc ttccatcggg gtatcggaca cgcggacttt
     2461 ggggaccgct gtttcgtttt tctcaaatgc atcgatatag cgttttttcg tttcccttaa
     2521 agccgcgctg tttaaagcac gcgttgcttc ttccaaagtg atttccttaa aaaccggacg
     2581 gacgggcgaa atttcgcttt cagacggcat caacccttca gcaatactcc ggtcgaatgg
     2641 gatgcggcgg ttgtcgatta cggcagttgc aatgtcttcc gtatcggcag cctcttcctc
     2701 cgcagcttct gcttcttccg tttccgcttc ttccgtcccg tttccactgt ctgaatatcc
     2761 gtcggatgcg gtcggaacat ccgcacgatt tgcagaagca ggcggaacgg gcctgtccgg
     2821 ttcggattcg tgcgccagat agtttcggca gaaacggacg acaccgacaa acagccaata
     2881 cactgccgtt ttgacggcat ggaaaagcat cagggcaagt tcgggaaaat cgggcatacc
     2941 gtctttgatt tcaggcagct ccgcctgttt ttcccctttc ttttcctgcc acgcagaaac
     3001 ctcgcgcatc cactcgcgtt cggattgtgc gcggacaaaa aacaagccgg caagcgcaag
     3061 caacaaaata acgatcaaaa ctatccaaaa catatgccgt ttcccgcaag acaaacgatt
     3121 aaacgggttt attttaacgt gtttccatcc caaaaaaaac aaccggtcgg caattctgcc
     3181 gaaaccgatt cggctttcgg tttgcccccg ccgacagtat aatagccgtc ccttccctta
     3241 atctttgcgg cattgccatg attttccaaa cagtctggtt ttcagatatg gtactgtcgg
     3301 tttcgtggat tgtccttatc ctgatactgg cagcttccgc gccgtccgca ttccgctcgc
     3361 ttgcccgcta ccgttccgcc cttccactgt gtaccgtaat tttttccgca gcatggtgtc
     3421 tgaacgcctc tgccggcggc ggacagcttg cacagatgaa ctaccacctg ctcgccgtca
     3481 atctggttgc actgatggtg ggcacttccg ccgccctttg gcttgccgcg ctgctgatgc
     3541 tgccctactg cctgctgttt gccgattctg ccggcgcata tccgccgaac gctttggtgc
     3601 tgattctgcc cgcgctggtc gtcaaccgcc tgtcgcgtat gctggtcaac cgcctgccgc
     3661 ccaatatttt catctttatt ttcgtcaacg gctttctcgc ttccgccgcc ggcattctgt
     3721 tgaccgggct ggtgctgacc ggtattttgg atgctgcaaa cgcttttccg tccgaaatat
     3781 tgtggacgac cgccctgccc gtctttattc tgctggcgtg ggcggaagcc ttcctcagcg
     3841 gcatttcaac tgccatattt gttgcactga agccccattg gatcaacact ttcgacgaca
     3901 accgctatct gaagtccgac cgcggcatat ggcggtagcc ttcagacggc ataggcagac
     3961 aaaaccatgc tttccaacat catccccctt tccatcggtg cggcactcgg tgcaacggcg
     4021 cgttggctgc tcaacctcgc cgttcctgcc gccatgtctc ccgccacggg caacctgttt
     4081 gccaactgga cgggcgcgct gctcatcggc atctttgccg aaaccatcaa tcacccgcaa
     4141 tggaagctgc tgctgattac cggttttctc ggcagcctga ccacgctttc cggattttca
     4201 ctggaaaccg taaccctgct ccaatcgaat cgtcctgctt cagcacttgc caatattttc
     4261 ctacacacgg caggttcgct gctgctgact tggctcgggc tgaagatagg ggcggcagtc
     4321 aaataaccgc aacaaattgc ctccctttgt tttgacataa tctataaatt agcttcgctt
     4381 gtttaaatac tggctgtgcc aa
//
LOCUS       DAWFKN010000123         4214 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFKN010000123 DAWFKN010000000
VERSION     DAWFKN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.123"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR46_002046"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR46_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0856898.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WGR46_002047"
     CDS             1384..2148
                     /locus_tag="WGR46_002047"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0856899.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WGR46_002048"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WGR46_002048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0856900.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         1002 a         1153 c         1156 g          903 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acctcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtt tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctggctcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcgaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattct gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagtgct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt gggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga gcaaatcaaa
     1501 aatatgccct tcgatattgc ggagttccgc gccgactttt tggaatgcgc gggcagtatc
     1561 ggcgaaatat tgcaccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc tcgttcccgt gttcggacga ttattatttt
     1681 gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 tccggcgaaa ccgccgtccg gtgcgccgtg gcaaatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcaaagcga aagagcttgc cgccaaaccg
     1981 attgttacga tgtcgatggg gcagacgggg gcggtcagcc ggcttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcgacact cgactgcctc gaaaacggag cagactgatt tcagacagca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgctacc tcggcggccc
     2221 actgctcgtc cttgccggtg caggcagcgg caaaaccggc gtgattactc aaaaaattaa
     2281 gcatttgatt gtcaatgtcg gctacctgcc gcataccgtt gccgcaatta cctttaccaa
     2341 caaagccgct gcggaaatgc aggagcgcgt tgccaaaatg ctgcccaaac cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccactcttt gggcatgaag attctgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttc gacgacttga tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgcaacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg aacatggaaa acctgcgcaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgagaac aaccccaagc tgttcaccaa
     3061 aaaactttgg tcgaaattgg gcgaaggcga gcccgtcaaa gtcgttgcct gccaaaacga
     3121 gcagcacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt ataccgcgga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gaaccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc gacagcatga aaaaaggcgg tccggaggat taaaccaaga tagggaaacg
     4201 aagccggaga caat
//
LOCUS       DAWFKN010000124         4143 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFKN010000124 DAWFKN010000000
VERSION     DAWFKN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.124"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..243
                     /locus_tag="WGR46_002049"
     CDS             <1..243
                     /locus_tag="WGR46_002049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856901.1"
                     /translation="RLKTEGGSLSSLPSDGISNAAFAPYAGGSPPSFQCLRRMPVASG
                     KPCAGWLCRLKQYEGMRFRRHPPVAEIPSASCPIIA"
     gene            complement(233..1426)
                     /locus_tag="WGR46_002050"
     CDS             complement(233..1426)
                     /locus_tag="WGR46_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0856902.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1423..2391)
                     /locus_tag="WGR46_002051"
     CDS             complement(1423..2391)
                     /locus_tag="WGR46_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0856903.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAVPQTGETEWKQD
                     TEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWP
                     KTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVERILEAAH
                     THRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGEDRYTDSV
                     NVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2402..3838)
                     /locus_tag="WGR46_002052"
     CDS             complement(2402..3838)
                     /locus_tag="WGR46_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0856904.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(3968..>4143)
                     /locus_tag="WGR46_002053"
     CDS             complement(3968..>4143)
                     /locus_tag="WGR46_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856905.1"
                     /translation="LVLIFVNPLYNVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAG
                     LWLCLNLNRHIQT"
BASE COUNT          864 a         1046 c         1340 g          893 t
ORIGIN      
        1 cgtctgaaaa cggaaggcgg cagtttatca tctttgcctt cagacggcat atcaaacgcc
       61 gcctttgcgc cgtatgccgg aggttcgccc ccgtccttcc aatgcttgag gcgaatgccg
      121 gttgcgtccg gcaaaccgtg cgcggggtgg ctatgccgtc tgaagcaata tgaaggtatg
      181 cgtttcagac ggcatccccc tgttgcggaa ataccgtccg cctcctgtcc gattattgcc
      241 tgattgcagc ggcgcggacg agttcttcga ggctgtcggc gagcatttcc gccgcgcgcc
      301 ggtagccttt ggcggagaag tgtacgccgt ctttggcggc ccatccttgg ttgagccagt
      361 ttttcatgct gcatatgccg cccatggcgt tttgccaaga ccagaacatg gtttgtccct
      421 gccgggcgac gcgccgctgc atatgttgga cttcggtcag gcggacgggg cgtgtgccgc
      481 atacgccgag cgtgtttttc agggattcgg gtgcgccgat gatgaggatg ccggcggcag
      541 gcaggctgtc gtggatttgg cggacggtat ccagccattt ttgttcagtg tcggcaatgt
      601 cgatgttgtc gccgaaggct tcgttggtgc cgtaggaaag gataaccaaa tcggcgccgg
      661 tttgggcaag gtcgttcata cggtcggcac gccatttcga ccaatgggtc aattgtgcgc
      721 cgttgatgcc catggcggaa acggtaatgc cgccggcggg attttcgatg ttgatgaagc
      781 cgatgtccca cggcatttcg gtctgtatgg tcaagggcag tgccgcgcgg gtatccagta
      841 cctgccagcc gccgccgttg gcggagacgg tgttgccgtt gacggtcagg gtttgttcgg
      901 cgagcagggg tttggcaaac agggaaatgc gctgtttgcc ggtcttgccg tccgatgcgg
      961 tcagggtcat gctgccgccg ctgccggtgt gggcgaggat gccgccgagc gggaagtctc
     1021 cgctattgtt cctgctggtg aggctttgcc agttaccgtt gtgccggacg gccgccatgc
     1081 gctgcccttt gacgttggcg gggtaaaccc agcctatgcc gccgtcgccc caagtttttt
     1141 gcaggcgttt gcgcaggctg tcggtaaaga agtcgccggc ggtatgcgag tcgccgattt
     1201 gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga ttggagtttt ttcatccaag
     1261 gcgaggcggc ggcgttgccg tagtcggtca gcagcccgtt tgtatcggtg tagggggcgg
     1321 acggggaaac ggtaacggtg tcgagactga cggaggcaac aggtagggcg tttgcctggg
     1381 ttgcggcgaa cagggcggaa aatgcgatga ggtgtttggg gttcatggct gtgtacttga
     1441 tggttgcgta cttggttcaa aaacgatttt ttccattatt tttgccgcca gcagtttttg
     1501 tccttcggcg gtaaagtgta tgccgtcctt gctgcggtag cggacgggtt tgccgttgac
     1561 gttgacggag tccgtgtagc ggtcttcccc gccgctcagg gtgtgcgcgg tgggaatcag
     1621 gatgattttg cctttcaaat gttccgaaag cagtttgtcg aggtagcgca tctgcccgtc
     1681 aagcttggct tttttcatgt aggggatgcc gagccagacg acttggacgc ggtgcgtgtg
     1741 tgcggcttca aggatgcgct cgacgcgttt caggtattct tgcgcccatt cgtcggaagc
     1801 aaatttgagg tagcgtttgc cgacgggaaa atcccacggg tcgttcgggc cgaggaagac
     1861 ggcgagtacg ctgatttcgg gatgtttttg cagggtttct tcaatcgttt tcggccagtc
     1921 gaagaatgag gggtaggaca gccccgtgct ttgtttgctg aggttgacgg attcgatgcc
     1981 gtattgctgt ttcaggcttt tttgcacgaa gggtgcaacg ccctgcatca gcgagtcgcc
     2041 ggcgaaaaag actttgtcgc cgctgcggac ggcggcggct tcggtgtctt gtttccattc
     2101 tgtttcaccg gtttgaggga cggcttcaga cggcatatct gccgagccgc cgtcttgagc
     2161 cgtcggcggc gtttcgccgg acaggaatgt tttgatgccg tctgaaaagg cgtaggcgtt
     2221 ttcttgcaac gccgcaccgc tccgccacca tccgtaggcg gcaagcggtt cgagcggact
     2281 gttgcggtgg taggtctgct gccagtaggc gttgatgggg ttttggctga accacacggc
     2341 aatcagggca gacatcaata tggaagtgaa aagggaaaga aagtgtttca tgtccaaacc
     2401 cttaaaaatt ggcataaata aagccgggaa tccccgaggg ggcgaatacg ataatcagca
     2461 gcaggacggc ggaaacgggg ataaaccaca gccacatcgg gattttttcc aaacctttga
     2521 ccgcgccgtc gaaagcgcgt tgcaggtaag ggtagagcag catcacggat gcaaacgagg
     2581 caagcagcag catatccgcc cgttgcggcg cattccagcc gttggcattg gcaaagaggg
     2641 cattgaaaac tgcgcctgca tcgtcggggt ttgcggtatt gaagacgacg aaggtcaggc
     2701 agacgaaatg gaaggtaaca aaccacgaga gcggcgcaag gtatttcaga cggcatagtg
     2761 cgttgcgtcc gaaatagcgg tcgcccgtgt tgagcagcac cagcgccgtg ccgtgcagcg
     2821 cgccccaaat gaggaagttc cagccgtagc cgtgccagat gcctgagagt accattgccg
     2881 ccatcaggtt gagctgtgtc cgtaaaaagc cttttttgct gccgcccaag gggatgtaga
     2941 tgtagtcgcg tatccaggtg gaaaggctga tgtgccattt gtcccaaaat gcacggatat
     3001 tcgcggcacg aagcggtgct gagaagtttt tgggcagtcg gaagcccagc agcattgcca
     3061 tgccgataac caaatcggaa tatccggaaa agtccaggaa gagttggaag gtatagccgt
     3121 acacgccgcc caatacgccc cagccgtcga attgggcggg gttttcaaat acgggcgata
     3181 cccagttttc cgccagcatc cccgccagcc accatttttt ggcaataccc agcagaatca
     3241 gggaaacggc gagtgcggga cggacgggcg aacgcggttg gcgggtacgg atttgcgcca
     3301 atgcgcccgc ctgttcgccg tccgcgcttt tgaatgcggc ggcgcggata atcgggccgg
     3361 aggtaacggt ggggaaaaaa ctcaggtgca gcagcagttc gtgccactcg aaacgcgcgg
     3421 cgtgcggcgc acggaagcag taaaccaaat aggcgattga ctggaaggtg taatacgaaa
     3481 gccccagcgg catcaggatg tcgattgcgc cgccttgtcc ggcatattgt gcaatcagcg
     3541 ggcggaaaaa gtcgaaatat ttgaaaaagc ccaagacggt cagcgagacg gcaatgccgc
     3601 accccagcca aaagcggcgc gtattttcgc gatcggaacg gagcagttcg cccaaaaggt
     3661 acacgcagga ggaataaagg acgacgattg ccgcaaatac ggggccgata tggtagagcc
     3721 agcccatacc ggcagccaaa agcagcaggt tttggacgga cgggtatttc gccaagcccc
     3781 agtaaatcgg caggaaggcg agaaagaaca gtgcgaactc gacagacagc agcggcataa
     3841 accatccttg gaataagaaa ataggcgtta aaacacggca aagcccgtta ttttaagcag
     3901 gggcggatat ttcttcaatc aaaatgccgt ctgaagggcg ggaggggctt cagacggcat
     3961 cgcgcggtca ggtttggata tggcggttca agttcaggca cagccacagc ccggccgccg
     4021 ccgcaagcat accggcgatg ccgatacggg aaatgccgac atattgcgtt acccaatgtc
     4081 ccaacagtgc gccgctgccg ataccgacgt tgtatagtgg attaacaaaa atcaggacaa
     4141 ggc
//
LOCUS       DAWFKN010000125         4026 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFKN010000125 DAWFKN010000000
VERSION     DAWFKN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.125"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            156..1673
                     /locus_tag="WGR46_002054"
     CDS             156..1673
                     /locus_tag="WGR46_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0856906.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1734..3614)
                     /locus_tag="WGR46_002055"
     CDS             complement(1734..3614)
                     /locus_tag="WGR46_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0856907.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQKGWQ
                     KGLHAVLFADYEFGLPLTGVDSERGGNLAMHWFADCADIDAASWLAQHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENRRCALLDRHLCRLKTAAQALN
                     LPLPDGCENQIKQYIAHLPDGAFRIKALLASDGISLSRAVLNHLADKQRVIISPTILP
                     AQNYLRRFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          833 a          910 c         1317 g          966 t
ORIGIN      
        1 ctataaatgt gaaatccgcc ctttgaaaat cgggcggcgg ttttttgcct gcccgctttc
       61 agacggcaaa gccggttttc acgggtttct gcctgttttt cggatggttt gacgtgcttc
      121 ggcggcgtgt ttgccagaaa ggtaaatgac agggtatgtt gtatttcaga tacggttttt
      181 tggttgtttg gtgtgcggca ggtgtttctg ccgcctatgg ggcggatgcg cccgcgattt
      241 tggatgacaa ggcattgttg caggtgcagc ggtcggtgtc ggataagtgg gcggaatcgg
      301 attggaaagt tgaaaatgat gccccgcgcg tggttgacgg ggattttttg ttggcgcatc
      361 cgaaaatgtt ggaacatagt ttgcgcgacg cgctcaacgg caatcaggcg gatttaatcg
      421 cttcgttggc ggatttgtat gccaagctgc cggattatga cgcggttttg tacggcaggg
      481 cgcgggcttt gctggcgaaa ttggcgggaa ggccggcgga ggcggtggcg cggtatcggg
      541 aactgcacgg ggaaaatgcg gcagacgagc ggattttgct ggatttggcg gcggcggagt
      601 ttgacgattt ccggctgaag tcggcagaaa ggcattttgc ggaggcggca aaattggatt
      661 tgccggcgcc ggttttggaa aatgtggggc gttttcggaa aaaaacggag gggctgacgg
      721 gctggcgttt ttcgggcggc atcagtccgg cggtcaatag aaatgccaat aatgccgcgc
      781 cgcagtattg ccggcaaaac ggaggccggc agatatgcag tgtcagccgg gcggagcggg
      841 cggcaggctt gaattatgaa atcgaggcgg aaaaactgac ggcgttggca gataatcatt
      901 atttgttgtt ccgttccaat atcggcggca cgagctatta tttcagtaaa aaatcagctt
      961 atgacgacgg gttcggcagg gcgtatttgg gttggcagta taaaaatgca cggcagacgg
     1021 cggggatttt gccgttttat caggtgcagt tgtcgggcag cgacggcttt gatgcgaaaa
     1081 caaaacgggt aaacaaccgc cgcctgccgc cgtatatgct ggcgcacgga gtcggcgtgc
     1141 agttgtccca tacttaccgc ccaaacccgg gatggcaatt ttcggtcgcg ctggaacatt
     1201 accgccaacg ctaccgcgaa caggataggg cggaatacaa taacggcagg caggacgggt
     1261 tttatgtttc gtcggcaaaa cgtttgggcg aatcggcaac tgtgttcggc ggctggcagt
     1321 ttgtgcggtt tgtgccgaaa cgcgaaacgg tgggcggcgc ggtcaataat gccgcctacc
     1381 ggcgcaacgg tgtttatgcc ggctgggcgc aggagtggcg gcagttgggc ggtttgaaca
     1441 gtcgggtttc cgcgtcttat gcccgccgca actataaggg cgttgcggct ttctcgacag
     1501 aggcgcaacg caaccgcgaa tggaatgtct cgctggcttt gagccacgac aagttgtcgt
     1561 ataaaggtat cgtgcccgcg ttgaattatc gtttcggcag gacggaaagt aatgtgccgt
     1621 atgcgaaacg ccgcaacagc gaggtgtttg tgtcggcgga ttggcggttt tgaatggtgg
     1681 aataatgccg tccgaacttt gtggaaacag gttcggacgg catttttgcg cgttcaggca
     1741 agggcggcgg caaatacgcc gcgcaaggcg ttggagaggc ggatttcttc ggcttcttgc
     1801 agtgtttttt gtgtgatgtg tgtttcgatt acttgatttg tttgcaaata tttttgcggt
     1861 tcgtccaata cggcttggcg cattatgccg tttaaaatgt ctaaatctaa agacggtgtg
     1921 agccattgcc cttggtattt gacgaacacg ttgcttctgc cgccttcgag caggatgccg
     1981 tctgaattga aaaacaggct gtcgaacgcg ccttgtgttt cggcggtttg ccacgcttgg
     2041 tcgaagaggg cgcggtgggt ggttttgaag cggcgcaggt agttttgtgc gggcaggatg
     2101 gtcggcgaaa tgatgacgcg ctgtttgtca gccaaatggt ttaaaacggc gcgggacagg
     2161 ctgatgccgt ctgaagcgag cagggctttg atgcggaacg cgccgtcggg caagtgggcg
     2221 atgtattgtt tgatttgatt ttcgcagccg tcgggcaggg gcaggttgag ggcttgggcg
     2281 gcggttttca gacggcatag gtggcggtcg agcagggcgc atcgcctgtt ttccacgcgc
     2341 agggtttcaa aaatgccgaa atcggggcgc aattcgttga ggaaacgggc tttccagccg
     2401 cattcgcgat attcggcggc ggggtcgctg tcgatgacga tgccggaacc gacaccgtac
     2461 acgccccgat acggcggatt ggagtcaaac ggatgcggcg ttgccccgcc ttgccctgcc
     2521 gttcgggctt gcgcgtttga atccgggccg ccgatgcctg aaacaatgcc gtctgaagcc
     2581 gaaacgggtt tgagcgacaa ggtgcggatg acgacgttga atatgccttc aaaccccaac
     2641 ccgcccgcgc aaggtttcag gtagccgatg ctgcccgtat aaagcccacg cgcttcggtt
     2701 tcgagcgatt cgataatctg catactcatt tttttgggcg caccggtgat gctgccgcag
     2761 gggaaggcgg cgcggaggat gtcggcgaac gaggtgtttg gcagggcttg ggcttggatg
     2821 gtgctggtca tctgccagac gctgccgaaa cgcgatactt taaacggctc gggcacgcat
     2881 actttgccgg tttgggcgat tttgccgagg tcgttgcgca gcaaatcgac aatcatcacg
     2941 ttttcggcgc ggtttttcgg gtcggtttgc aactcggcgg cgcggcgttc gtcttgtccg
     3001 tcgcccaaaa tcggcgcagt gcctttcatc ggttcggtgc tgatggtgcc gtctgaagcg
     3061 atgttgagga agagttcggg cgagaaacac agcgtccacg cggattgccc ctccacatcg
     3121 ggcaggtggg acaagacggc ataggggacg ggctggcgca ggcggcggta gaggctgacg
     3181 ggattgccgt aggcttgcag gtgcaggcgg gtggtgtagt tgatttgata ggtgtcgccg
     3241 cgccggatgg cttcgtggat ttggcggatg tggtcgaggt aatcggtttc ggatacggag
     3301 gattgcggcg tggaaatgcc ggcggggatg ccgtctgaat gttgggcaag ccagcttgcg
     3361 gcatcgatgt cggcgcagtc ggcaaaccag tgcatggcaa gattgccgcc gcgttcggaa
     3421 tcaacccccg tcagcggcaa accgaattcg tagtctgcaa acaacacggc atgcagccct
     3481 ttttgccagc ccttttgcag cgcgccgtcc aaagcatcga gttcttcggg acggaaaaaa
     3541 cggctttcta catgattttg atagcgtttt gcgcggccgc ttacggcatc gtcaaacagg
     3601 gcgaaatagg gcatggcaat tcggggcaaa tgttttgatt atacgctctt tttacacata
     3661 ttttcagacg attcggcaaa tatcggcaaa atgtaatttt atgtagagaa agtggggggc
     3721 gaaggtgtaa aattgtgaaa acaaattccg atttccaacc tgaaatacaa taaggagacc
     3781 tttatgacag accaccagct gcaaccgttt gaaaacgtag aattaggcga aaagcaagac
     3841 cagctccaag tatagtggat taacaaaaac cagtacagcg ttgcctcgct ttagctcaaa
     3901 gagaacgatt ctctcaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
     3961 tcttcggctt cgttgtcttg tcctgatttt tgttaatccg ctataaagac cgtcgggcat
     4021 ctgcag
//
LOCUS       DAWFKN010000126         3909 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFKN010000126 DAWFKN010000000
VERSION     DAWFKN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.126"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(80..898)
                     /locus_tag="WGR46_002056"
     CDS             complement(80..898)
                     /locus_tag="WGR46_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0856908.1"
                     /translation="MKGMNKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(978..2228)
                     /locus_tag="WGR46_002057"
     CDS             complement(978..2228)
                     /locus_tag="WGR46_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0856909.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGR46_002058"
     CDS             complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGR46_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0856910.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2888..3700
                     /locus_tag="WGR46_002059"
     CDS             2888..3700
                     /locus_tag="WGR46_002059"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0856911.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          867 a          942 c         1163 g          937 t
ORIGIN      
        1 ccgaaggtaa gggaggggtg tgtccactgt gccgccgcat ttgccgcgtg cggaagatac
       61 ggataacaac cccttggaat taaagggaga agttgcggaa aatatccata tcaggctgtg
      121 ggccgtactt atttacggta ttttcgtatg ccgaaataaa atatttcttg aataaaccgt
      181 aatcgccggt ttcgtaatag gctacgacag cctcggcata ggcgggataa ctgtcggggg
      241 cgaatacgca aggaaaaaaa ccggagcgca tcagcgacaa ggtcatacag tttctggcag
      301 tacgtttatt gcaatcttta aaatattgaa gatacgccaa attattatgg agataaacgg
      361 cgcggtcgaa gggatttcct atttttgggg cttcttgaag caaccatttt aattccgtat
      421 ccaaactttg cgggttggat aaaggcgtgt agtctgtccc gcttatagta acgctgtcgc
      481 ggcggactac gccgccggag cctttttcca gtaagttttc cgaaatcagg ctgtgggtgg
      541 tttttaagaa atccaaccag tcaaacggat cggggcggtc caaaccggac agcaggtgcc
      601 ggtaactttc cctcaggttg attaacatta cggcatccga atataacttc ccgcctgcgg
      661 tttgcccgag tttaagcaat gcctgcgtat cgtattgatt ataagtgttt ccttccaatt
      721 tggcagaact gtacacaaaa tcgataccga tttcttcaat attacgcacg gagtccgtta
      781 aaacagataa aggaagccga tcggacaatt cggtcaggta ttcttgttcg ccggctgtaa
      841 aaatggggcg gatatttaaa aaatcctgtt ggtatctcaa tttattcata cctttcatca
      901 taaaaacgcg gtttggggcg gaagtaggtt tatgcctgcc ttcatccgac aactatattg
      961 aaacaccgcc ttttcaatca accccgcttc aatacggatg tattgaggta gcgttggaca
     1021 cccgaggcaa tggattgggc gcactgccga cggaaggatt cgctgcccag cagcttctct
     1081 tcggcaggat tggacaggaa ggcggtttcg accaggatag acggcatatc gggcgcgcgc
     1141 aaaacggcga aattggcttc gtccaccctg cctttgtgca agtggttgag cctgcccaat
     1201 tcttcaagca ccagtttgcc gagtttgcgg ctgtcgcgca gcgtggcggt ttgggtcatg
     1261 tcgagcaggg cggtatcgac attgcggttg ccgctggtcg gtacgccgcc gaccgcgtcg
     1321 gcattatttt gggtttgttc caagaatttg gcggcagagc tggtcgcgcc tttggtgttc
     1381 agcatataaa cccccgttcc gcgcgcggag gggctggtga aagcgtcggc gtggatggag
     1441 acgaatacgt ccgcccgccg tgcgcgccct ttggcgacac gcacgcccaa tgggatgaac
     1501 acgtcttcgt tgcgcgtcat aaagacgttg taacccaagg cttccaattg ttttttggtt
     1561 tcgcgggcga tggagaggac gacgtgtttt tcctgcaaac cgcccgggct gacggcgccg
     1621 gggtcttcgc cgccgtgtcc ggggtcgatc ataatgacgg gtctgcgccc gtttttgccg
     1681 cgttcggttt gtgcgggagt gttttgagcg aggtcggctt caggggagcc gcgacgggtt
     1741 ttgttcaggc tgccgttgag cagcgccatc atcggatcgt cggcatccat cccttgcgga
     1801 tagagatcga cgacgaggcg gtttttaaag ccgccgacgg gtgggaggga aaaaacttgc
     1861 gcgtaggaag gctgtttcaa atcgatgacg aggcggacgg tgtcgggcgt gttctgaccg
     1921 gcgcgtatgc tgcggataaa ggggtcgtcc gccataactt tttgggaaag tccgtgcaat
     1981 acggtattga tgttcgcgtt ttgtatatcg acgaccagcc tgcccggatt gtcgagcgtg
     2041 aagtgttggt atttgagcgc ggcggtgctt tccagcgtca ggcgggtgta ggtgtgcgac
     2101 ggccatatcc gtacggcggt aaactgcggg gcgcgtaccg ttttggcaac ggcggatgcg
     2161 atggggctta gggcgaacag tgtgccggcg gtgcggcgga tgatttgtct tcgtgtcagt
     2221 ttggtcatag cggcaggctt tcgcgtcctc gttcggtatg ggcggtcagc aggcattttc
     2281 tgccgtcgcc gtcgtgtgtc aatgttgcgg tgatgtcggc aggcggggta aattccccac
     2341 cttgttgcgg ccattcgatc aggcagacgc tgtttgcggc aaacagttcg tccaatccgg
     2401 catcttccca ttcttcgggg aacgagaagc ggtagaggtc gaaatggtgc aaggtgaagc
     2461 gttccagcag ataagattcg acgatggcgt aagtcgggct tttgactgcg ccctgatgac
     2521 ccaatccgcg caggatgccg cgtgtcagcg tggttttgcc cgcacccaaa tccccctcga
     2581 gataaatgac cagcggcgcg tttaaacggg aagaccacgc cgcacccaaa tcgagtgtgg
     2641 cggcttcgtc ggcaaggaat cgggagatag agggtaaatc agacatggaa acggtttgtt
     2701 gtaaggtcta gggtattatg ggcagttttg caggttttgc aaactttgca cccgaggggc
     2761 ggatgcttct tgtccgagca ttataacagc caaatccgcg ttctgctttc agacggcaac
     2821 ggctgtcaag aaaaagcggc gcgtgtacaa tacgcggatt gtatgtttag gacagattgg
     2881 aaaaaggatg gaaaatatcg gcaggcagcg acccatcggc gtttttgact cgggaatcgg
     2941 cggtttgacc aatgtgcgcg cgctgatgga gcggctgccg atggagaaca tcatttattt
     3001 cggcgacacg gcgcgcgtgc cttacgggac gaaatctaag gcgaccatcg aaaatttctc
     3061 gatgcagatt gtcgattttc tgctggaaca cgatgtcaag gcgatggtta tcgcgtgcaa
     3121 cacgattgcg gcggtggcgg ggcagaaaat ccgtcaaaag acgggcaata tgcctgtgtt
     3181 ggacgtgatt tccgccggcg cgaaagccgc gctggcaacg acgcgcaaca ataaaatcgg
     3241 catcatcgcc accaatacca cagttaacag caatgcttat gcgcgcgcca tccacaggga
     3301 caaccccgac acgctcgtcc gcacgcaggc tgcgccgctg ctcgtccctt tggtggaaga
     3361 gggctggttg gaacacgaag ttacccgcct gaccgtatgc gaatacctca aaccattgct
     3421 tgcagacggc atcgatacgc tggtgttggg ctgcacgcac tttcccttgc tcaagccctt
     3481 aatcggcagg gaggcgggca atgtcgcgtt ggttgattcc gcgattacga ctgccgaaga
     3541 aaccgcacgc gtccttgcgc aggagggatt actcaatacc gacaacaaca atcccgacta
     3601 ccgtttttac gtcagcgata ttcctttgaa attcagaacc atcggcgagc gttttctggg
     3661 caggacgatg gagcagattg aaatggtgtc tttgggttaa aacgatgacg gaaagctgcc
     3721 cgagattaca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatct agacctgtcg
     3781 gtgcggaaac ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt
     3841 cgcgggaatg acgggatgca ggtttctgtg cggatgtatt cggattccct cctgcgcggg
     3901 aatgatggg
//
LOCUS       DAWFKN010000127         3859 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFKN010000127 DAWFKN010000000
VERSION     DAWFKN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.127"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGR46_002060"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGR46_002060"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0856912.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGR46_002061"
     CDS             1439..1924
                     /locus_tag="WGR46_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0856913.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR46_002062"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR46_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0856914.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          781 a          940 c         1080 g         1058 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ttgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgttgg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWFKN010000128         3844 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFKN010000128 DAWFKN010000000
VERSION     DAWFKN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.128"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGR46_002063"
     CDS             complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGR46_002063"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0856915.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            645..1514
                     /gene="tehB"
                     /locus_tag="WGR46_002064"
     CDS             645..1514
                     /gene="tehB"
                     /locus_tag="WGR46_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0856916.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGR46_002065"
     CDS             complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGR46_002065"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0856917.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            2431..2628
                     /locus_tag="WGR46_002066"
     CDS             2431..2628
                     /locus_tag="WGR46_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856918.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(3672..3747)
                     /locus_tag="WGR46_002067"
     tRNA            complement(3672..3747)
                     /locus_tag="WGR46_002067"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3711..3713),aa:Glu,seq:ttc)
BASE COUNT          855 a          928 c         1113 g          948 t
ORIGIN      
        1 tctggattcc cgcctgcgcg ggaatgacgg tggaggacga tgccgtctga agcccattag
       61 cggtttcaga cggtattttt gtcggtgcgg cagtcaggaa aactgcgccg cgccacggtt
      121 tgccaaatcg tcggcgcgtt cgttttccgc gtgtcccgcg tgtcctttca cccaagtcca
      181 gctgacttgg tgccgtccga ccagcgcgtc gagttctttc cacaagtcgt cgtttttgac
      241 gggctgtttg gcggcggttt tccagccgtt gcgcttccaa ccgtgtatcc agttttccat
      301 gccgtttttg acgtattgcg agtcggtgca gatgatgacg gtgcagcggc gtttgagcga
      361 tttcagtcct tcgatgacgg cggtcagttc catacggttg ttggtggttt gcgcttcgcc
      421 gccgaaaagt tctttttcgt ggctgccgta gcgcattaat acgccccagc cgcccgcgcc
      481 gggattgcct ttgcacgcgc cgtcggtgta aaggtaaacg gtttggttca ttattgtgcc
      541 tttatgtggt gtccggcgtg attataagcg gtttgtgcgc cgtgccgtct gaaagattgc
      601 ggcggattaa aataggcata taatgtgcaa ctttattttt gaatatgaag gagcggatag
      661 tgggtcaaag cggcgagttg ttttgtttcg gacaaatgcc cgtatggaag gcggaaaacc
      721 tgccggaaat tttgttgtcg ggttattcgt ccgaaggagg ggaatgggtc tgcctgaatg
      781 tgttgcaggg cgatgtcgaa gttcgtgcac cggacgggtc ggcagaagtt tggtcggcag
      841 acggcggcga ttgtgtgttt gcgccgcagc aggtgttttc tgtcaaaccg aaaacggacg
      901 atgccgaaat ccgtttgtcg ctgtattgcg cggcggcaga ctattttcac aaaaaatacg
      961 ggatgagtgc cacgcattct gcggtcgcgg cggcacagga taccgtaccg gcgggcaggg
     1021 cgttggatat gggctgcgga caggggcgca acgcgctgtt tcttggtttg aaggggtttg
     1081 aagttactgc agtcgatcac aatcccgccg ccttggcaaa cgtggcggag ctggcagagg
     1141 cggagggttt gaacgtccgc acgctggaat atgatttgaa cgccgccgcc ttgcagggtg
     1201 agtttgatta tattgtggca acagtggtgc tgatgttcct gatgccgcag cgcgtgcctg
     1261 acgtgattgc cgatatgcag gcgcatacgg cggcgggcgg gtacaactta atcgtatcgg
     1321 caatggatac ggcggatttc ccttgtccga tgccgttccc ttttaaattt aaagagggag
     1381 agctgaagga ttattatcgg gattgggagc tggtcgaata taaagaagaa ttgggcgcga
     1441 tgcacgccaa agacgaaaac ggcaatccga tacggtttaa atttgtaacg atgctggcga
     1501 aaaagcctga ataatcctca aaaatgccgt ctgaacattt tgtttcagac ggcattttta
     1561 cggttagtcc cgataggccc aatgattgac cgggccgtgt cccgcgccga tttccaaagg
     1621 gcttgagatt gccgccgtga tgtaggactt ggcagtccgt acggctttgc aaacgtctaa
     1681 gccttttgcc aactcggcgg taatgcaggc agaaaacgtg cagcccgtgc cgtgcgtgtg
     1741 ggcggtcgga aagcgcgggc tgtccaattc cagcgtttcg ttttgcgtaa acagccaatc
     1801 cgtgcagcgt ccgctgctgc tgccattcaa atgtccgcct ttgatgacga catttttgac
     1861 accgtaatca agcaggattt ttgccgcacg ttccgcatct ttacggtttt cgatatgcac
     1921 gccggtcagg gcttccgcct cgggcaggtt gggggtcaat acatccgtat cgggaagcag
     1981 caggcgcgtc agtgccgcaa cggcggaatc ctgcaacagc ggcgcaccgc ctttggcaat
     2041 catcacaggg tcgagtacgc gcctgccgaa gtcgcagtgt ttcagcttgt cggcaacgca
     2101 ttcgatgatt tccgccgtgc cgagcattcc tattttgtag gcgcggatgt caaagtcttc
     2161 cctgattgct tggatttgtg cggtgatggt ttcggtcggg acgagatgaa ccgccgacac
     2221 gcccaaggta ttttgcgcgg taacggcggt gatgacgcac gttccgaaca cgccgcgcat
     2281 ctgaaacgtt ttcaaatccg cctgaatgcc cgcaccgccg cccgaatccg aaccggcgat
     2341 ggtaagcgtt tgcacaaaag agcttttcat ttgttcctcc ttaatattgt gtccgataaa
     2401 aagggctaat gcctgtaaaa aagcacggcg atgccgtctg aaaaccgcag cgcgggggtt
     2461 tgtggtgcat tatatgccgt attgcaggac aaggcacagg gcagggcggc ggggtgcatc
     2521 gtagcaactg tatttttgcc cgacggggtg aaaaatacag ttgctacacc cactgataat
     2581 gaaaaaagag agcaatgccg tctgaaccgc gtgttgcggc gagcataaac cggcacgctt
     2641 tattcgtcat ttccacaaaa acagaaacct aaaattccgt cattcctacg aaagcgggaa
     2701 tccagtgtgt tcggtttcag ttgtttttgg gtttcgggtg atttccaaat catcattccc
     2761 gcgcaggcgg gaatctagcc cttggggcag cggcaatatt tataaagccg tctgaaaatt
     2821 cagaagttct agattcccgt tttcacggga atgacgaagg gttgtgggaa tgacgaagtt
     2881 tcagacggca tcgccccgcc ccgtcattcc cgcataagcg ggaatccaaa ccattgggca
     2941 gagcagcctt aaaggttata aaagactcgt cattcccacg aaagtgggaa tccagacctg
     3001 tcggcacgga aacttatcgg ataaaacggt ttcttcaacc ctacgctcta gattcccgct
     3061 tttgcgggaa tgacgaagtt tcagacggca tcaaatgctt tgtcaggctt cagacagcat
     3121 cgtccaccct ctgccgtcct tcccgcgcag gcgggaatcc aatgtgttcg gtttcggttg
     3181 tttttgggtt tcgggtaatt tccaaatcgt cattcccacg aaagtgggaa tccagaccat
     3241 tgggcggcgg aaatattcaa aagtcgtctg aaaattcaga ggttctagat tcccgttttc
     3301 acgggaatga cgaagggttg tgggaatgac gaagttttag acggcattga atactttgat
     3361 gctttggtgg gctgaagccc accctacaat atagagaaaa tgccgccaaa cggtatggaa
     3421 gcggaaacaa tggtggggct ggcttcagaa acaatggtag ggtgggcttc agcccaccgc
     3481 tttagacggc atccgcgcac cgtcattccc gcgcaggcgg gaatccagcc ttgtcggcac
     3541 ggaaacttat cggataaaac ggtttcttca accctacgct ctagattccc gttttcacgg
     3601 gaatgacgaa gagtgtgaga atgacggttc ataaataaaa aaacctgctt aaaacaagca
     3661 ggtttttgaa ttggcacgcc cacggggatt cgaaccccgg ttgccgccgt gaaagggcag
     3721 tgtcctaggc ctctagacga tgggcgcgtg ggaatgggca ttatacatac ttctagaaat
     3781 ttagcaatac gatgccgtct gaaacttcgt cattcccgcg aaagcgggaa tctagaatct
     3841 ccaa
//
LOCUS       DAWFKN010000129         3820 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFKN010000129 DAWFKN010000000
VERSION     DAWFKN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.129"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            374..718
                     /locus_tag="WGR46_002068"
     CDS             374..718
                     /locus_tag="WGR46_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0856919.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICIP
                     ETLQTQMTHTRI"
     gene            715..3576
                     /locus_tag="WGR46_002069"
     CDS             715..3576
                     /locus_tag="WGR46_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ0856920.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1034 a         1035 c         1017 g          734 t
ORIGIN      
        1 cgaagccgca gacagtacag atagtacgga accgattcac ttgttaaaga atcgttctct
       61 ttgagctaag gcgaggcaac gccgtactgg tttttgttca tccactataa ctagggaaat
      121 tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta taaacaaaat
      181 aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa tataaaaacg
      301 ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt gtcagattca
      361 aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca aaccggctgc
      421 catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg gcggcacttc
      481 tgcgcccgac ttcaatgcag gcggcaccgg tatcggcagc aacagcaggg caacaacagc
      541 gaaatcagca gcagtatctt acgccggtat caagaacgaa atgtgcaaag acagaagcat
      601 gctctgtgcc ggtcgggatg acgttgcggt tacagacagg gatgccaaaa tcaatgcccc
      661 cccccgaatc tgcataccgg agactttaca aacccaaatg acgcatacaa gaatttgatc
      721 aacctcaaac ctgcaattga agcaggctat acaggacgcg gggtagaggt aggtatcgtc
      781 gatacaggcg aatccgtcgg cagcatatcc tttcccgaac tgtatggcag aaaagaacac
      841 ggctataacg aaaattacaa aaactatacg gcgtatatgc ggaaggaagc gcctgaagac
      901 ggaggcggta aagacattga agcttctttc gacgatgagg ccgttataga gactgaagca
      961 aagccgacgg atatccgcca cgtaaaagaa atcggacaca tcgatgtggt ctcccatatt
     1021 attggcgggc gttccgtgga cggcagacct gcaggcggta ttgcgcccga tgcgacgcta
     1081 cacataatga atacgcatga tggaaccaag aacgaaataa tgtctgcagc catccgcaat
     1141 gcatgggtca agctgggcga acgtggcgtg cgcatcgtca ataacagttt tggaacaaca
     1201 tcgagggcag gcactgccga ccatttccaa atagccaatt cggaggagca gtaccgccaa
     1261 gcgttgctcg cctattccgg cggtgataaa acagacgagg gtatccgcct gatgcaacag
     1321 agcgattacg gcaacttgtc ctaccacatc cgtaataaaa acatgctttt cattttttcg
     1381 gcaagcaatg acgcacaagc tcagcccaac acactgaccc tattgccatt ttatgaaaaa
     1441 gatgctcaaa aaggcattat cacagtcgca ggcgtagacc gcagtggaga aaagttcaat
     1501 ggctccaacc attgcggaat tactgccatg tggtgcctat cggcacccta tgaagcaagc
     1561 gtccgtttca cccgtacaaa cccgattcaa attgccggaa catccttttc cgcacccatc
     1621 gtaaccggca cggcggctct gctgctgcag aaatacccgt ggatgagcaa cgacaacctg
     1681 cgtaccacgc tgctgacaac ggctcaggac atcggtgcag tcggcgtgga cagcaagttc
     1741 ggctggggac tgctggatgc gggtaaggcc atgaacggac ccgcgtcctt tccgttcggc
     1801 gactttaccg ccgatacgaa aggtacatcc gatattgcct actccttccg taacgacatt
     1861 tcaggcacgg gcggcctgat caaaaaaggc ggcagccaac tgcaactgca cggcaacaac
     1921 acctatacgg gcaaaaccat tatcgaaggc ggttcgctgg tgttgtacgg caacaacaaa
     1981 tcggatatgc gcgtcgaaac caaaggtgcg ctgatttata acggggcggc atccggcggt
     2041 agcctgaaca gcgacggcat tgtctatctg gcagataccg accgatccgg cgcaaacgaa
     2101 accgtgcaca tcaaaggcga tctgcagctg ggcggcgaag gtacgctgta cacacgtttg
     2161 ggcaaactgc tgaaagtgga cggtacggcg atgaccggcg gcaagctgta catgtcggca
     2221 cgcggcaaag gggcaggcta tctcaaccgt accggacaac gtgttccctt cctgagtgcc
     2281 gccaaaatcg ggcgggatta ttctttcttc acaaacatcg aaaccgacgg tggtctgctg
     2341 gcttccctcg acagcgtcga aaaaacagcg ggcagtgaag gcgacacgct gtcctattat
     2401 gtccgtcgcg gcaatgcggc acggactgct tcggcagcgg cacattccgc gcccgccggt
     2461 ctgaaacacg ccgtagaaca gggcggcagc aatctggaaa acctgatggt cgaactggat
     2521 gcctccgaat catccgcaac acccgagacg gttgaaactg cggccgccga ccgcacagat
     2581 atgccgggca tccgccccta cggcgcaact ttccgcgcag cggcagccgt acagcatgcg
     2641 aatgccgccg acggtgtacg catcttcaac agtctcgccg ctaccgtcta tgccgacagt
     2701 accgccgccc atgccgatat gcagggacgc cggctgaaag ccgtatcgga cgggttggac
     2761 cacaacgcta cgggtctgcg cgtcatcgcg caaacccaac aggacggtgg aacgtgggaa
     2821 cagggcggtg ttgaaggcaa aatgcgcggc agtacccaaa ccgtcggcat tgccgcgaaa
     2881 accggcgaaa atacgacagc agccgccaca ctgggcatgg gacacagcac atggagcgaa
     2941 aacagtgcaa atgcaaaaac cgacagcatt agtctgtttg caggcatacg gcacgatgcg
     3001 ggcgatatcg gctatctcaa aggcctgttc tcctacggac gctacaaaaa cagcatcagc
     3061 cgcagcaccg gtgcggacga acatgcggaa ggcagcgtca acggcacgct gatgcagctg
     3121 ggcgcactgg gcggtgtcaa cgttccgttt gccgcaacgg gagatttgac ggtcgaaggc
     3181 ggtctgcgct acgacctgct caaacaggat gcattcgccg aaaaaggcag tgctttgggc
     3241 tggagcggca acagcctcac tgaaggcaca ctggtcggac tcgcgggtct gaagctgtcg
     3301 caacccttga gcgataaagc cgtcctgttt gcaacggcgg gcgtggaacg cgacctgaac
     3361 ggacgcgact acacggtaac gggcggcttt accggcgcga ctgcagcaac cggcaagacg
     3421 ggggcacgca atatgccgca cacccgcctg gttgccggtc tgggcgcgga tgtcgaattc
     3481 ggcaacggct ggaacggctt ggcacgttac agctacgccg gttccaaaca gtacggcaac
     3541 cacagcggac gagtcggcgt aggctaccgg ttctgacgga caggaagcag acagccgcaa
     3601 agatcacggt ctttgcggct gtttcttatg aaaagaaaac cctattccaa ttgcctgctt
     3661 ctattgtttc aagacttctt ccaaagattc ggcattaatc agatgtatag cggattaaca
     3721 aaaatcagga caaggcggcg aagccgcgga cagtacaaat agtacggaac cgattcactc
     3781 ggtgcttgag caccttagag aatcgtttct ttgagctaag
//
LOCUS       DAWFKN010000130         3743 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFKN010000130 DAWFKN010000000
VERSION     DAWFKN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.130"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            1071..1394
                     /locus_tag="WGR46_002070"
     CDS             1071..1394
                     /locus_tag="WGR46_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0856921.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            1508..3685
                     /locus_tag="WGR46_002071"
     CDS             1508..3685
                     /locus_tag="WGR46_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0856922.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          931 a         1246 c          945 g          621 t
ORIGIN      
        1 gctttcacaa agcctgtgtg cactgaaaac tgaaaggtcg tctgaaaacg gagctttcga
       61 aggaagcaga gtttctgcga agctaaaacc gaagctgcaa aaaatcgaaa agcagcctgc
      121 acgttgaaaa taccgctcaa gcaaacctac ggtttgccgc cctctcccta gccctctccc
      181 acagggagag gggattgggg tgcaggctgt ctttaagctt caggcgattt ttaactcttg
      241 ttgaagctgc gatttcagaa gctccgtttt agcttcgcag aaactccgct tccttcgaaa
      301 gctccgtttt cagacgacct tttggaacac cacaggcaca cgcatcgaac ggctgaatca
      361 aagttcagac cgatggcagc ccgcacccga gtttatgcgg caaacagcga ggctacggca
      421 acccgtcccc tctccctgtg ggagagggtt agggagaggg cagtaagccg taggcttaca
      481 tcaaagtcga taacgctttc acaaagcctg tgtgcactga aaactgaaag gtcatctgaa
      541 aacggagctt tcgaaggaag cagagtttct gcgaagctaa aaccgaagct gcaaaaaatc
      601 gaaaagcagc ctgcacgttg aaaatgccgc ccaagcaaac ctgcggtttg ccgccctctc
      661 cctagccctc tcccacaggg agaggggatt ggggtgcagg ctgtctttaa ggttcaggca
      721 aatttttaac ttcgttgaag ctgcgatttc agaagctccg ttttagcttc gcagaaactc
      781 cgcttccttc gaaagctccg ttttcagacg accttttgga acaccacagg cacacgcatc
      841 gaacggctga atcaaagatt cagaccgatg gcagtccgca cccgagttta tgcggcaaac
      901 agcgaggcta cggcaacccg ccccctctcc ctgtgggaga gggttaggga gagggcggta
      961 agccgcaggc ttgcatcaaa gtcgataacg ctttcgttac aactccgccc actgaacgaa
     1021 gccaaaacga caaaccgcat cgtaaaccac ccaacccata ggagaacccc atgcaaaacg
     1081 aaaccatcaa cctgaaacag caccttgccg ccatcaaaga atactggcag cccgaaatca
     1141 tcaaccgcca cgggttccaa ttccgtttgg tcaaactttt gggcgattac ggctggcata
     1201 cgcacggata cagcgacaaa gtgctgtttg ccgtggaggg cgacatggcg gtggacttcg
     1261 ccgacggcgg cagcatgacg atacgcgagg gcgagatggc ggtcgtgccg aagtcggtgt
     1321 cgcaccgccc gcgttcggaa aacggctgct cgttggtgct gattgagttg tccgacccgt
     1381 ccgaggccgt ctgaaaacga agtttccgaa ggaagctgag tttctgcgaa gctaaaagca
     1441 gcctgcacct tcaatcaata tgccgaaaat acaaccccac cgcacaccaa cacacaaagg
     1501 aaatcccatg acacgcttca aatattccct gctgtttgcc gccctgttgc ccgtgtacgc
     1561 gcaggccgat gtttctgttt cagacgaccc caaaccgcag gaaagcactg aattgccgac
     1621 catcaccgtt accgccgacc gcaccgcgag ttccaacgac ggctacactg tttccggcac
     1681 gcacaccccg ctcgggctgc ccatgaccct gcgcgaaatc ccgcagagcg tcagcgtcat
     1741 cacatcgcaa caaatgcgcg accaaaacat caaaacgctc gaccgcgccc tgttgcaggc
     1801 gaccggcacc agccgccaga tttacggctc cgaccgcgcg ggctacaact acctgttcgc
     1861 gcgcggcagc cgcatcgcca actaccaaat caacggcatc cccgttgccg acgcgctggc
     1921 cgatacgggc aatgccaaca ccgccgccta tgagcgcgta gaagtcgtgc gcggcgtggc
     1981 ggggctgctg gacggcacgg gcgagccttc cgccaccgtc aatctggtgc gcaaacgccc
     2041 gacccgcaag ccattgtttg aagtccgcgc cgaagcgggc aaccgcaaac atttcgggct
     2101 ggacgcggac gtatcgggca gcctgaacac cgaaggcacg ctgcgcggcc gcctggtttc
     2161 caccttcgga cgcggcgact cgtggcggca gcgcgaacgc agccgcgatg ccgaactcta
     2221 cggcattttg gaatacgaca tcgcaccgca aacccgcgtc cacgcaggca tggactacca
     2281 gcaggcgaaa gaaaccgccg acgcgccgct cagctacgcc gtgtacgaca gccaaggtta
     2341 tgccaccgcc ttcggcccga aagacaaccc cgccacaaat tgggcgaaca gccgccaccg
     2401 tgcgctcaac ctgttcgccg gcatcgaaca ccgcttcaac caagactgga aactcaaagc
     2461 cgaatacgac tacacccgca gccgcttccg ccagccctac ggcgtagcag gcgtgctttc
     2521 catcgaccac aacaccgccg ccaccgacct gattcctggt tattggcacg ccgacccgcg
     2581 cacccacagc gccagcgtgt cattgaccgg caaataccgc ctgttcggcc gcgaacacga
     2641 tttaatcgcg ggtatcaacg gttacaaata cgccagcaac aaatacggcg aacgcagcat
     2701 catccccaac gccattccca acgcctacga attttcccgc acgggcgcct atccgcagcc
     2761 atcatcgttt gcccaaacca tcccgcaata cggcaccagg cggcaaatcg gcggctatct
     2821 cgccacccgt ttccgcgccg ccgacaacct ttcgttgata ctcggcggca gatacagccg
     2881 ttaccgcacc ggcagctacg acagccgcac acaaggcatg acctatgtgt ccgccaaccg
     2941 tttcaccccc tacacaggca tcgtgttcga cctgaccggc aacctgtcgc tttacggctc
     3001 gtacagcagc ctgttcgtcc cgcaatcgca aaaagacgaa cacggcagct acctgaaacc
     3061 cgtaaccggc aacaatctgg aagccggcat caaaggcgaa tggcttgaag gccgtctgaa
     3121 cgcatccgcc gccgtgtacc gcgcccgtaa aaacaacctc gccaccgcag caggacgcga
     3181 cccgagcggc aacacctact accgcgccgc caaccaagcc aaaacccacg gctgggaaat
     3241 cgaagtcggc ggccgcatca cgcccgaatg gcagatacag gcaggttaca gccaaagcaa
     3301 aacccgcgac caagacggca gccgcctgaa ccccgacagc gtacccgaac gcagcttcaa
     3361 actcttcact gcctaccact ttgcccccga agcccccagc ggctggacca tcggcgcagg
     3421 cgtgcgctgg cagagcgaaa cccacaccga ccctgccacg ctccgcatcc ccaaccccgc
     3481 cgccaaagcc cgcgccgccg acaacagccg ccaaaaagcc tacgccgtcg ccgacatcat
     3541 ggcgcgttac cgcttcaatc cgcgcgccga actgtcgctg aacgtggaca atctgttcaa
     3601 caaacactac cgcacccagc ccgaccgcca cagctacggc gcactgcgga cagtgaacgc
     3661 ggcgtttacc tatcggttta aataaggtcg tctgaaaacg gagtttctgc gaagctatag
     3721 tggattaaca aaaaccagta cgg
//
LOCUS       DAWFKN010000131         3709 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFKN010000131 DAWFKN010000000
VERSION     DAWFKN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.131"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="WGR46_002072"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="WGR46_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0856923.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="WGR46_002073"
     CDS             1586..3604
                     /locus_tag="WGR46_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0856924.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          777 a          830 c         1114 g          988 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccggaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgacaagg aaaagcgcgc tatgtacgac cagtatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggcggtg
      541 cgcagggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgcg
      601 cccagcctga ttatcagggt gaggacgttc aagtcggtat cgaaatcacg cttgaagaag
      661 ccgcaaaagg tgtgaagaaa cgcatcaata ttccgactta tgaagcgtgt gatgtctgta
      721 acggcagtgg cgcgaaaccg gggacatccc cggaaacctg cccgacttgc aaaggttcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcagca gacttgtccg acctgccacg
      841 gtgcgggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg cggaataagg
      901 cggtcaagac ggtggaagtc aatattcccg ccggtatcga tgacgggcag cgtatccgtt
      961 tgagcggcga aggcggaccg ggtatgcacg gtgcgcctgc tggcgacctg tatgtaaccg
     1021 tccgcattcg ggcgcataag attttccaac gcgacggtct ggacttgcat tgcgaactgc
     1081 cgatcagttt tgccacggct gctttgggcg gggagttgga agtgccgacc ttggacggaa
     1141 aggtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgc gtgaagggta
     1201 agggtgtcaa atctttacgc agcagcgcga ccggcgattt gtactgccat attgttgtcg
     1261 aaacgcctgt caatttgacc gaccgtcaaa aagagctttt ggaagaattt gagcggattt
     1321 ctaccggctt ggaaaaccaa acaccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagccgtat cggggaatct ccctgatacg
     1441 gctgttttgt ttgtttaaaa atagttttta ttttcaatgg ggtatggggc agggtgggat
     1501 aactgttttt aactgttctt tttaaaactt gacatcatgg cgcgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccgggtcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggtgcattg atcactgtaa tttttactgc
     1681 gtccaacgtt tacctcggtt tgaaagtcgg gctgaccttt gcctcgtcga ttccggcggc
     1741 ggtaatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcttcgg cggcgggtac gctttcgacc atcatcttcg tcctgcccgg
     1861 tttgctgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg tgattttcac cattccgctg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gataaccgtc agggcggcag cggcatcaaa gagctggcgg ccggcggtgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgc gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcgatttt ccagctgccg atgggctttt cactggcatt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggtatc gccatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggagaaa gtgcgtttta tcggtgcggg
     2401 gactattggc attgcggcgg tttggacgct gttgatgctg ctcaagccga tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctgcggaac gcgccgaaca
     2521 agatttgtcg cctaaagcca tgattttttg ggtgctggcg atgatgtttg ttttaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcaggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtgtccgt
     2761 cgtcgttatt tcactggttt tgctgctggt aggcgaatcc ggaggtttgt tggcggatga
     2821 ggctaaccgc aaatttttgc tggcactgac tttgttttgc ggctcggcag taatctgcgt
     2881 ggcttcgatt tccaatgaca acctgcaaga cttgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gttggtgcgc tggttatttc
     3001 gcccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 catggatgcg gcgcaggctt tggcagctcc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc tcgaatgggt ctacatcttt accggcatcg cgatcggcgc
     3181 ggtattaatc gtcgtcgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgtatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg atcggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgct ccgcttgcgt tgaatctgca aaactgggat gccgccgctt cttggttggg
     3541 tttggcgttc ttcgttaccg ggatgttttt ctttgcacag cgcgtactga aggcgggcaa
     3601 gtaggctgtc ggaaaaaatg ccgtctgaag cgttcagacg gcatttttat ctggaaagcg
     3661 gaaggcggag cttttcggct tgcgcccacg ttttgccggc aaggtcttt
//
LOCUS       DAWFKN010000132         3368 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFKN010000132 DAWFKN010000000
VERSION     DAWFKN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.132"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(63..2234)
                     /locus_tag="WGR46_002074"
     CDS             complement(63..2234)
                     /locus_tag="WGR46_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0856925.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIGR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSRTGYAGNVKGPNH
                     TQITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGKFKISDKKPDPNDPTKE
                     IDKDAAEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          749 a          775 c          956 g          888 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcgcggc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac gtgttgaaca cgttgttaac cgaaagatta
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 ccttggcgta caacgttttc caatttgccg ctgcggtctt tttgacctgc caccaatatc
      301 gaacccacgg ctttttgaac atagcgtccg cgccagccga tttccagatt cggattttgg
      361 aagcggtagg caagggaggc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gcttacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cgttgacggc ttcacggacg gcgacagagt cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ttgccagaag tagctgccgt tggcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg ctttcgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttacc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcgt gggttttcac tttgaagcgg
      901 tcgtaacgca gcccgccggt cagggtaaag ccgtcaatct cgtgaatggc ttcgatatac
      961 gcgccggtat cggttttggt cggattggac agtttgtagg aatggacaag atccatatct
     1021 ttcttgtctt tcgctttttc tgcagcgtcc ttatctattt cttttgtggg atcgtttggg
     1081 tctggtttct tatcggagat cttaaatttg ccgttcaaaa acgcttgcgg tttgatttcc
     1141 tgatggcggt agttgatacc gtatttcagc agggtttgtt cggcaaggcg gctgtcgaag
     1201 ttgaagttca taccccgagt ggtgatttgg gtatggttgg ggccttttac attgcctgcg
     1261 tagccggtgc ggctgtcatc ggcggaatag cgttcttttt ccaacacata ggcgttggca
     1321 tccagttttt cgacaaagcc caggttttta cccgtgtacg ccaagttggt gttggattgg
     1381 gtagtttcgc ggtaagcagg ggcttggcgg ccaatattta tccgtgaact tttgtcgccg
     1441 acggtaaatt cttcacgcac agtgcggatg cctcggtgtt ggtctttcat atggctcaac
     1501 acgatgcggt ggtcgccgtc gccgaagctt gttccgattt tggcgaggta gctgcgtttg
     1561 tccagcgcgc tgtacggtac ggttttgccg ccgttaacat tgcggaaacc tttgccggct
     1621 tcgtaatctt tttcatcgtt gcggttgtaa gagaacaaac cgtcgaagtt gccctctttt
     1681 ccgaatacgc ttgcgccgta gcttacgcct tcgttgctgg caaagccgct gttgaggcgc
     1741 acgccccagt ttttatccaa gcctttgagc aggtcttggg catcgacggt tttggcgata
     1801 atcgcgccgt tggtcgcgcc gataccggca gaggcggaac ccgcgccttt ttgtacggaa
     1861 acgactttaa ccaaagcggg atcgacaata aatctgcctt ggtggtaaag gatttggctg
     1921 tcggaatagg cgttgtccac cttgatgtcg acagagttct gacccatacc gcgcagcgtc
     1981 aggaattggg acgtgccgtt gccgccgccg aaatcgatgg acggctcttc ttttaagagt
     2041 tcgcgcatat cggttgcggt gctttcgtct ttttgttgaa gcgtaacgat gttggtacgg
     2101 attttgctgc cttggcggtc gccttttacg gtaacggtat ccaatgcgac attggcatta
     2161 ttttctgccg catgggcaaa acctgccgcc agggtaagcg agagcaggct gaggcggaaa
     2221 aacggggcgt tcatttgttc gtccttttga gtgtatgaag ggaagtaaat ccaaaccgtt
     2281 aaggtttggc aggataagaa aaataataat tatttttgtt tatattaacg agagggggat
     2341 ttgtgtaaag ctgattatcg tttttatttg cgaagtgttg ttttttgttg acaggttttg
     2401 tcggaaatgt atagtggact aacaaaaatc aggataaggc gacgaagccg cagacagtac
     2461 aaatagtacg gcaagacgag gcaacgctgt actggttttt gttagtccac tataaaaaac
     2521 ggcggaaata tagtggatta acaaatgcgg gaatgacgaa aagcaacctt tccgcgtcct
     2581 tcccgcgaaa gcgggaatct agaaccccta acgcggcagg aatctatcgg aaatgactga
     2641 aaccgaacag actggattcc cgcctgcgcg ggaatgacga agcctgtgcg ggaatgacga
     2701 ggcctgcgcg ggaatgacga aaagcaacct ttccgcgtcc ttcccgcgaa agcgggaatc
     2761 tagaacccct aacgcggcag gaatctatcg gaaaaaaccg aaaccgaaca gactggattc
     2821 ccgcctgcgc gggaatgacg aggcctgcac gggaatgacg aagcctgcgc gggaatgacg
     2881 aaaagcaacc tttccgcgtc cttcccgcaa aagtgggaat ctaggaactc aaagctgcaa
     2941 gaatttatca gaaacaactg gaactcaaaa aaactagatt cccgcctgcg cgggaatgac
     3001 gaagggttaa agaaatgatg agaaacggcg gtaatttatc gggaataacc gaaaacgaac
     3061 ggaaatcagg acaaggcggc gaagccgcag acagtacgga taggacggaa ccgattcact
     3121 tggtgcttga gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgacgtact
     3181 ggtttttgtt catccactat aacagcaacc ctgtcgccgt cattcccgcg aaagcgggaa
     3241 tccagtccgt tcagtttcgg tcatttccga taaattcctg ctgcttttca tttctagatt
     3301 cccacttttg cgggaatgac ggcggaaggg ttttggtttt ttccgataaa ttcttgaggc
     3361 attgaaat
//
LOCUS       DAWFKN010000133         3221 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFKN010000133 DAWFKN010000000
VERSION     DAWFKN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.133"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..639
                     /locus_tag="WGR46_002075"
     CDS             <1..639
                     /locus_tag="WGR46_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0856926.1"
                     /translation="INRQLTEKGLKIEKASAAVVDATIIQTAGSTQRQVIEVDEEGQI
                     NGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLE
                     GLPKGTTVYADKGYDSKENRQHLKEHQLLDGIMRKACRNRPLSETQTKRNRYLSKTRY
                     VVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            743..1300
                     /locus_tag="WGR46_002076"
     CDS             743..1300
                     /locus_tag="WGR46_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0856927.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGLCGELGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(1376..2344)
                     /gene="ispH"
                     /locus_tag="WGR46_002077"
                     /gene_synonym="lytB"
     CDS             complement(1376..2344)
                     /gene="ispH"
                     /locus_tag="WGR46_002077"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0856928.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(2373..2870)
                     /locus_tag="WGR46_002078"
     CDS             complement(2373..2870)
                     /locus_tag="WGR46_002078"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0856929.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          793 a          941 c          798 g          689 t
ORIGIN      
        1 attaaccgac aactgaccga aaaaggctta aaaatagaga aagcatccgc cgccgtcgtt
       61 gatgccacca ttattcagac cgccggcagc acacagcgtc aggtcataga agtcgatgaa
      121 gaaggacaaa tcaacggcca aaccacaccg agtaaggaca gcgatgcccg ttggatcaag
      181 aaaaacggcc tctacaaact cggttacaaa caacataccc gtaccgatgc ggaaggctat
      241 atcgagaaac tgcacattac ccccgccaat gcccatgagt gcaaacacct gtcgccgttg
      301 ttggaaggac tgcccaaagg tacgaccgtc tatgccgaca aaggctacga cagtaaggaa
      361 aaccggcagc atctgaaaga acatcagtta ctggacggca ttatgcgcaa agcctgccgc
      421 aaccgtccgc tgtcggaaac gcaaaccaaa cgcaaccggt atttgtcgaa gactcgttat
      481 gtggttgaac agagcttcgg tacgctgcac cgtaaattcc gctatgcgcg ggcagcctat
      541 ttcgggctgc tcaaagtgag tgcgcaaagc catctgaaag cgatgtgttt gaacctgttg
      601 aaagccgcca acaggctaag tgcgcccgct gccgcctaaa aggcagcccg gatgcctgat
      661 tatcgggtat ccggggagga ttaagggggg atttgggtag aattaggagg tatttggggc
      721 gaaaatagac gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa
      781 aggtctcagc ctgcccgaaa tcatccgcga cctgctcgaa atgccgtctg aaaccgccat
      841 tgccgacatc acacgcactt attccgcaca ttacctcaat cccaacaacc gcaatatgac
      901 gctctttccc gatgccctgc cctgtctgga caagctcaaa gcacaaggat actggcttgc
      961 cgtcgccacg ggcaaagggc gggcgggctt ggacaacgcc atcagccaaa ccgccaccgg
     1021 cggctattgg ctcgccaccg cctgcgcggg ggaatatccc tccaaaccct cgcccgaaat
     1081 ggtattcgga ctctgcggcg aactgggact cgatccgaaa gaagcattgg tcgtcggcga
     1141 tacggcgcac gacctgcata tggcggcaaa cgcaggcgcg gcggcagtcg gcgtggccac
     1201 cggcgcacat tcgcgcgaac agctccttag cgcaccgcat ctcgccgtat tggacgactt
     1261 gtccgaactg cccggttttc ttgcacaaca ttacgcctga ttggtttccg catcagacac
     1321 tcgaaacaaa tgccgtctga agcctgttca gacggcattt gtgttgccca aacattcaac
     1381 gtttgcacaa atcgggtttg gtttcgccct cgcggcgcag ctctttgggc aggacgaaca
     1441 caatgctttc ttccgcaccc tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg
     1501 ttgccagtac ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt
     1561 tgtttttgcc ctcaaaccat gcgcgttgca ggtagcctgc attatccacc atatacgcat
     1621 cgattccgcg cgatgccgcc acttcgcgca agcggttgct gttggacgaa ttgggcgaac
     1681 cgaccacaat cacgatgtcg cactgttctg ccaactcttt gacggcggtt tgccggttgg
     1741 tcgtcgcata gcagatatct tccttgtgcg gattgcggat attggggaaa cgcgcgttca
     1801 gcgcggcgat gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga
     1861 gtttgtcggg gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca
     1921 ttttgcccgg cgcaagctgc cccatcgtgc cttcgacctc gacgtgcccc ttatgcccga
     1981 tcatgatgat ttcacagtct tgggcatcca gtcgggcgac ttccttatgc actttcgtca
     2041 ccagcgggca agtcgcatca aacacgcgga aaccgcgctc cgccgcttct tgctgcaccg
     2101 ccttcgatac gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt
     2161 cttcaataaa caccgcacct ttttcacgca ggttgtccac gacgaatttg ttgtgaacga
     2221 cttcgtggcg cacataaatc ggcgcgccga actcttccaa agcacgttcg acaatactga
     2281 ttgcccgatc cacaccagcg cagaagccgc gcggattggc aaggatgatg gttttctcgt
     2341 tcataagccc ggtatttcgt tttcagacgg catcaatatt tttcttcttg ggttttacgg
     2401 tggacgatgt tgtccaacac cgccaacacc gcaccgacgc agataaagct gtcggcaata
     2461 ttaaaggcgg gataaaacca attttgccaa taaaacaata agaaatcgac gacatgaccg
     2521 tgtatcaggc gatcgatgac attgcctaac gcaccgccga taatcatcgc cgcacccgtt
     2581 ttgccgaggg ttgcaaactc atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc
     2641 accgccagca ccaaaaaaaa gtatttttgc cagccgccct gatcggcaag gaagctgaac
     2701 gccgcacccg ggttgtacac caaggtcaaa tcgaaaaagg aaggaatgac attgacgcgt
     2761 tcccgatact gaaacgacga cagcaccgcc cacttcgacc actggtccag cacgatggcg
     2821 gcaagtgcca atacccaata gcgcgtttta cttgaaacag atgaagacat atttttcaac
     2881 agccggtaaa agagtaccat tttacccgaa aacccccttt cctgtacccg aaacggcaaa
     2941 tgccgtaatc ttaaaacccg tcattcccga caacaccgta atctcgaaac ccgtcattcc
     3001 cgcgcaggcg ggaatccaga cctgtccgca cagaaactta tcggataaaa acagttgccc
     3061 aaaccccgcg ttctatagtg gattaaattc aaaccagtac ggcattgcct cgccttagct
     3121 caaagagaac gattctctaa ggtgctgaag caccgagtga atcggttccg tactatttgt
     3181 actgtctgcg gcttcgttgc cttgtcctga tttaaattta a
//
LOCUS       DAWFKN010000134         3084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFKN010000134 DAWFKN010000000
VERSION     DAWFKN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.134"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            113..2878
                     /locus_tag="WGR46_002079"
     CDS             113..2878
                     /locus_tag="WGR46_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0856930.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYNPYKNYNNQELQKYIEDHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDNTLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
     gene            2908..>3084
                     /locus_tag="WGR46_002080"
     CDS             2908..>3084
                     /locus_tag="WGR46_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856931.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYE"
BASE COUNT          874 a          765 c          745 g          700 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcgcgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 ccccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 gacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agtggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atttggaacg gcgcaagcag cgatattttg tacaagaggg
      901 tgctttgaaa ttcaattccg acagcggaaa atgggagcgg gatttacaaa ggcaacagtg
      961 gaaatacaat ccgtataaaa attacaacaa ccaagaacta caaaaataca tcgaagacca
     1021 tgataaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggcaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 acttttaaag gattttgaaa cctacaacaa cgcgaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgcg aaaccgagtt gcaaaccacg ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtcctga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca agaaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggattgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggaa tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagta cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggcaga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagttc gttcgacccg aaagacaagg acgaagaagt
     2761 aacgtgtaat gatgataaca cgttatgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgataacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggttatttt
     3061 tggttgtttc gggtaattta tgag
//
LOCUS       DAWFKN010000135         3041 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFKN010000135 DAWFKN010000000
VERSION     DAWFKN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.135"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            45..2303
                     /locus_tag="WGR46_002081"
     CDS             45..2303
                     /locus_tag="WGR46_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0856932.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFSLLKGGPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2316..2981
                     /gene="pgmB"
                     /locus_tag="WGR46_002082"
     CDS             2316..2981
                     /gene="pgmB"
                     /locus_tag="WGR46_002082"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0856933.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
BASE COUNT          766 a          934 c          790 g          551 t
ORIGIN      
        1 gatttcacca ttccctcaaa tcaatccaaa caggagcttc ataaatgtac acaagaatca
       61 tggaaatcag cccttggacg ctgcgttcgg caaaactgga aaaagaacac aaacggctgc
      121 aagagagcct gaccagcttg ggcaacggct atatgggtat gcgcggcagc tttgaggaaa
      181 cctattccgc cgacagccac ttaggcacct acatcgccgg cgtgtggttc cccgacaaaa
      241 cccgcgtcgg ctggtggaaa aacggctatc ccaaatattt cggcaaagcc atcaacgcgt
      301 tcaatttcag caaagtcaaa atctttgtcg acgggcagga agtggacttg gcgaaaaacg
      361 acgttgccgg cttctccgtc gaactcgata tgcggcacgg cgtgttgcgc cgctcgttta
      421 ccgtattcgg tgtgcgtttc aatgtgtgca aattcctgtc cgtcgcgcaa aaagagctgg
      481 cggtcatccg ctgggaagcc gtatccgttg acggtaaaac ccaccaagtc cgcatcgatt
      541 ccatcatcga tgccgacgtg aaaaacgaag actccaacta cgaagaaaaa ttctggcagg
      601 tattggacaa aggcgtttca gacagtctct cctacattgc cgcccaaacc gttgccaacc
      661 ctttcggcgt ggaacaattt atcgtcaacg ccgagcaaac tttcgccggc agcttcaaag
      721 ccctcggcgg cagccaaacc gactggcaag tctccaattc tttcgaggtc gaagtcggcg
      781 gcacgcccaa aacctttgaa aaacgggtta ttgttaccac cagccgcgat tatcagggct
      841 tggaagcagt gaaagccgca ggccgcgcct tgtcggaaaa agtcgcaggc gttgcgtttg
      901 aaaccttgct ggacgcgcac aaagcaggct ggctgcaccg ttgggaaatc gccgacgtgg
      961 tcatcgaagg cagcgacgaa gcgcagcaag gcatccgttt caatctgttc caactgttct
     1021 ccacctacta cggcgaagat gcgcgcctga acatcggccc caaaggcttt accggcgaaa
     1081 aatacggcgg cgcgacctat tgggataccg aagcctatgc cgtaccgctc tacctcgcac
     1141 tggcggaacc cgaagttacc cgcaacctgc tgcaataccg ccgcaaccaa ctgccgcagg
     1201 cgcagcacaa cgcgcgcgaa cagggcttgg cgggcgcact ctatccgatg gtaacgttta
     1261 cgggtatcga gtgccacaac gaatgggaaa tcaccttcga ggaaatccac cgcaacggcg
     1321 cgattcctta cgccatctac aactacacca actacaccgg cgacgagggc tatcttgcca
     1381 aagaaggctt ggaagtcttg gtcgaagtgt cccgcttctg ggcggaccgc gtccacttct
     1441 ccaaacgcaa cggcaaatac atgattcacg gcgtaaccgg cccgaacgaa tacgaaaaca
     1501 acatcaacaa caactggtac accaacaccc tcgccgcctg ggtattggac tacacccgcg
     1561 aagccctggc gaaatacccg cgcccggatt tgaacgtgcg tgccgccgag ttggaaaaat
     1621 gggcggacat cagcgcgaat atgtaccgtc cgcatgacga agaactcggc gtattcgtcc
     1681 aacacgacgg cttcctcgac aaagacatcc gtcccgtgtc cgcgctttcg cccgacgatt
     1741 tgccgctcaa ccagaaatgg tcgtgggaca aaatcctgcg ttcgcccttc atcaagcagg
     1801 cggacgtgtt gcaaggcatc tacttcttcg gcgaccgttt caatatcgac gaaaaacgcc
     1861 gcaacttcga tttctacgaa ccgatgaccg tgcatgaaag ctcgctgtcg ccctgtatcc
     1921 actctattct cgccgccgaa ctgggcaaag aagaaaaagc cgtggaaatg taccggcgca
     1981 ccgcccgcct ggacttggac aactacaaca acgacacaga agacggcctg catatcacct
     2041 ccatgaccgg ttcgtggctc gccatcgtcc aaggtttcgc ccaaatgaaa acttggggcg
     2101 gcaaactcag cttcgcaccg ttcctgccgg gcgcgtggac aggctatgcc ttccacatca
     2161 actaccgagg ccgtctgatt aaagtcgccg tcggcaaaga aaacgttgtc ttcagcctgc
     2221 tcaaaggcgg gccgctcgat ttgcaggtgt acggcaaaga catcacgctc aacggcagcc
     2281 acaccgccgc gttggaaaaa taaggagggc gcaaaatgac gtttaccgca gtcctcttcg
     2341 atctcgacgg cgtgattacc gataccgccg aataccacta ccgcgcgtgg aaaaagctcg
     2401 ccgaagaact gggcatcggc atcgaccgca agtttaacga acagctcaaa ggcgtgtcgc
     2461 gcgacgattc gctcaaacgc atcctcgcgc acggcggcaa aaccgtcggc gaagccgagt
     2521 tcgccgaact gacccgccga aaaaacgaca actacgtcga gatgattcag gcagtcaaac
     2581 ccgaagacgt gtaccccggc attttgccgc tgctggagac attaaaagca aacggcaaaa
     2641 aaatcgccct cgcgtccgcc agtaaaaacg gcccgttcct gctcgaacgc atggggctga
     2701 cccacttttt cgacactgtc gccgaccctg ccgccgtcgc gcattccaaa cccgcccccg
     2761 acatcttcct cgccgccgcc gagggcgtgg gcgcggacat ccgccactgc atcggcattg
     2821 aagacgccgc cgccggcgtt gccgccatca aagccgccgg cgcactgccc atcggcgtag
     2881 gcaaagccga agacttgggc agcgacatcg cgctggtgtc cgagaccgcc gggctgacct
     2941 acgcctacct gcaaaacgtg tgggcgcagt cgggcaggta aagcgcgtca gataaagtgt
     3001 taaggaagta aaaggccgtc tgaacagtgt ttcagacggc c
//
LOCUS       DAWFKN010000136         2989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFKN010000136 DAWFKN010000000
VERSION     DAWFKN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.136"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            358..690
                     /locus_tag="WGR46_002083"
                     /pseudo
     CDS             358..690
                     /locus_tag="WGR46_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(907..1701)
                     /locus_tag="WGR46_002084"
     CDS             complement(907..1701)
                     /locus_tag="WGR46_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0856934.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1745..1849
                     /locus_tag="WGR46_002085"
                     /pseudo
     CDS             1745..1849
                     /locus_tag="WGR46_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1910..2635
                     /locus_tag="WGR46_002086"
     CDS             1910..2635
                     /locus_tag="WGR46_002086"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0856935.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          794 a          900 c          686 g          609 t
ORIGIN      
        1 taagggggta tttgggtaga attaggaggt atttggggcg aaaacagccg aaaacctgtg
       61 tttgggtttc ggctgtcggg agggaaagga attttgcaaa ggtctcgggc tatgccggct
      121 gctgccgccc attgcacctt cgccgaatcc tgccgcccac cgccgaagcc ctcatgcgtt
      181 cgcggtacag cgcgtatgtg ctactatagt ggattaacaa aaaccagtac agcgttgcct
      241 cgccttagct caaagagaac gattctctaa ggtgctgaag caccgagtga atcggttccg
      301 tactatttgt actgtcttcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac
      361 atcatcgcta ctaccgttcc ggcacaacaa acattcctcg atgccgccga actgatgcag
      421 tggagcagag aaaccgaatg gctgggcttg aatgtcatcg cacaccgcaa cttcggcaaa
      481 cagcacgccc aagtcgaatt tgaagcctac ttccgagacg gacaacaccg atccgcgcat
      541 cacgaactgt ccggcttcat caacatcggc ggacaatggt attttatcga tcctaccgtt
      601 ccgcatcctg cgatgaaaca accctgcatt tgcggatcgg gcaaaaaatt caaagcctgc
      661 tgcggcaaat atctgaaacc tgtcgcataa aaatgccgtc tgaacgttca gacggcattt
      721 tcaacacgca aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      781 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      841 ggattgtacc cgaattaatg ttaagaaacg ttaatcgtga aaatatatta acaaacctgt
      901 tgaaacctat tggttttccc gtatccactc gacccagcgt tcaaacagct tcggttcgag
      961 cgcggcgacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
     1021 agtcgtcagc ctgccgcccg cctcctcgaa aatcaacgcg ccggcggcat aatcccacag
     1081 cttctgcccg ccgtggacat aaacatcata acgcccgcac gccagataac accagtccaa
     1141 cgtactgctg cccatactcc gtatcgttcc aaaaggcgcg agcgtactca tgcggctgga
     1201 cagtttgccc gaacgcaaat atttgatttc cacgcccgca atcgcctcat tgagtttttt
     1261 atccacgaga cgcaaaggca ggcgggttcc attaagaaac gcaccctgcc cccgttcggc
     1321 ataaaaacat tcgccgctga ccgggttgta gattacgccc aattccgcac gcccgttgcg
     1381 gacaaacgcc accgacaccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1441 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1501 ttcctgccgc gacatttcct cgcccaacat cgggcaatcg atcagaagcg gcaacgcggc
     1561 ggcaaatgcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccat cttccttgcg
     1621 gcaagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1681 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1741 cgtatatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga cagtacaaat
     1801 agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat aatgtccgtt
     1861 tccatcacgc cgctgcgaca gattataacc gtccgaaccg ccaaaaacta tgccccgatt
     1921 ccacctgccc gaaaaccttt ccgtcggaca aaccgtcgcc ttgcccgaca acatcgtccg
     1981 ccacctcaac gtcctgcgcg tccgccccaa cgaaaacatc accctcttcg acggcaaagg
     2041 caaggcacac gccgcacggc tgtctttttt ggaaaaacgc cgcgccgaag ccgaaatcct
     2101 gcacgaagac acaaccgaca acgaatcccc gctcaacatt acactgatac aatccatctc
     2161 ctccggcgat cgcatggatt tcaccctgca aaaaagcgtc gaactcggcg taaccgccat
     2221 acagcccgtc atcagcgaac gctgcatcgt ccgcctcgac ggggaacgcg ccgccaaacg
     2281 cctcgcacgc tggcaggaaa tcgtcatctc cgcgtgcgaa caaagcggca gaaacaccgt
     2341 tccccccgta ctgcccatca tcggctatcg tgaagcactc gacaaaatgc cgtctgaaag
     2401 caccaagctg attatgagca tcaaccgcgc ccgcaaactc ggcgacatcc gccacccatc
     2461 cggcgcaatc gtctttatgg tcgggcccga aggcggctgg acagaacagg aagaacaaca
     2521 ggcatttgaa gccggctttc aggcggttac actcggcaaa cggattttac gcacagaaac
     2581 cgccccactc gccgccctcg ccgcgatgca gacgctttgg ggcgatttcg cataaacaga
     2641 aatgccgtct gaaacccgtt cagacggcat tttgcagccg attaagatag taggttcaaa
     2701 taagatttcc cgtgtcgtca ttcccgcgaa agcgggaatc tagaaacgaa aaactacagg
     2761 gatttatccg aaacaacaac cctctccgcc gtcattcccg cgcaggcggg aatctagaaa
     2821 cgaaaaacta cagggattta tccgaaacaa caaaccctct ccgccgtcat tcccgcgcag
     2881 gcgggaatct agaaatttaa cgttgcggtg atttatcgga aatgactgaa actcaacgga
     2941 ctggattccc gcctgcgcgg gaatgacaaa gtggaagttg cccgaaact
//
LOCUS       DAWFKN010000137         2953 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFKN010000137 DAWFKN010000000
VERSION     DAWFKN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.137"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(139..1449)
                     /locus_tag="WGR46_002087"
     CDS             complement(139..1449)
                     /locus_tag="WGR46_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0856936.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1761..2183
                     /locus_tag="WGR46_002088"
     CDS             1761..2183
                     /locus_tag="WGR46_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856937.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2269..2766
                     /locus_tag="WGR46_002089"
     CDS             2269..2766
                     /locus_tag="WGR46_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0856938.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2871..2953
                     /locus_tag="WGR46_002090"
                     /pseudo
     CDS             2871..2953
                     /locus_tag="WGR46_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          808 a          714 c          716 g          715 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataccac cttgtcttta ttggctttat cgataaacag
       61 gataagacct gaaaaaagcc gatacgcctt tttggtgtac cggctttgcc atactgttct
      121 gcttcagacg gcatcgcttc attttgcctt taatacttct tcgtccagcg atttcaacca
      181 ttccagcttt tcgccgattt tgatttccaa cccgcgcggg acgggttggt agaagtccgg
      241 ttcgtccaaa ccgtcgggca tatagctttc gccggcggcg taggcgttcg gttcgtcgtg
      301 ggcgtagcgg tattcgcgtc cgtagcccaa ttccttcatc agcttggtcg gggcgttgcg
      361 caggtggacg ggcacttcgt cgctggcgtt ttctttgacg aagtggcgca tttggttgta
      421 tgccttgtag cccgcgttgg atttggcggc ggcagcaagg tacagcacgg cttgagccaa
      481 agccagttcg ccttcgggcg agcctaagcg ttcgaaggtg gcggcggcat cgttggcgat
      541 ttggaaggcg cgcgggtcgg caagcccgat gtcttcccaa gcgatacgca cgatgcggcg
      601 ggcgaggtag cgcgggtcgg tgccgccgtc gagcatacgg cagaaccaat acagcgcggc
      661 gttcggatgc gaaccgcgca cggatttgtg cagggcggag atttggttgt agaagctctc
      721 gccgcctttg tcgaaacggc ggatttgcgc cccgagactg tcggcgagaa attcggcggt
      781 taagtttttc agacgacgtg tatcggcggc gcgtaaaagt tgttccaaca aattcaacaa
      841 tctgcgcgca tcaccgtcgg cggtattgac gaacagctcc tgcgcgtcgg cttcaatcgt
      901 gaaatcccgg tattcgggca aagccaaaac tttggcaacc agctttttga ggccgtctga
      961 agacaagggc tgcaaaacat acacctgcgc gcggctcaac agcgcgggat tgacttcaaa
     1021 cgacggattt tccgtcgtcg caccaataaa ggttagcaaa ccgctttcga catgcggcaa
     1081 aaacgcgtcc tgctgcgcct tgttgaagcg gtggacttca tcgacaaaca aaatcgtcgc
     1141 gcgtccctgc tgcaaagcga tttcggcttt atcgattgcc tcgcggatgt ccttcacgcc
     1201 ggaaaacacg gcggaaacag gcaaaaactg ggcattgaaa ctctgcgcca aaatccgcgc
     1261 caacgtcgtc ttgcccacgc ccggcggccc ccacagcaac atagaatgcg gcttgccgcc
     1321 ttctaccgcc acgcgcaaag gtttaccttc gccgatgagg tgttcctgcc ccaccacgtc
     1381 gtcaagcgta tgcggacgca atcgttcggc aagcggcgcg tcgggttctc gggcaaacaa
     1441 atcggtcata acggctccgt caacaggttt tcaaacaata tgattatacg gcagggaacg
     1501 gcggcgtgcc gcatacggat tccgcccctc cgtttgcctt aagccgatat taggcgcata
     1561 ctggaaaaga cgagagactt cacacaatat atccggcacg gagaccgatt ccgcatcggc
     1621 atgacaatac ccaaatcagc gtttcaatta aacattaagg agactaaaat agaaaatttg
     1681 ctttatctac cattgctttg ttgattcaat cggcattatg ttttgaggcg gaagcccatg
     1741 aatatactaa tattcaagag atggaatggg tgtctttatt ttctgatccg caaagagacg
     1801 atgatagtct tataaccctt aaagatgaaa aaatcactgt aaaaaactat attgtgcctt
     1861 ggtggaaaaa aggtgaaaac tttagaaaat tagaacttat taccactggt aaaaatgata
     1921 atggaaattt aacggtaaat ggaatttacg gcatccaatc ttttgcagac tctcaattgg
     1981 gatatacgcc tgaattattg gtaaaaagca ataatgatat tctatttaaa aataatttcc
     2041 aatctgttga cgtgaagccc cattccggaa atatccattt ggatgccaaa ggaaaaattc
     2101 aatttaattt gaatgttccc gatgttgatg gggtacaaga tgtagccagt ggaaaaaaat
     2161 accgacaaac aatagtgcct tagtgcggta tgtcaacact atcagccccc cgatgaaaat
     2221 gacaaaggca gcatatcctt tcattcaaaa acaggcaatt tgttcaatat gaacgttaaa
     2281 gttcataaag acacacacca tttcggtatt cacaacctca acaataccga tgttcatctg
     2341 actgccgaag acagcgataa tgtgattgag attaaagggg aactcgccga ccttatgccg
     2401 acaggaaatg ttgagagtac aagtttttta aggggcattg aaacggcttt taaatccaat
     2461 accgtattaa cggggaataa caataaaata tccatcgcca ctgagggcaa gggcttggga
     2521 ttgggcgtaa atggtctcca gtccaactcc ccctattccg gacacacgac gggcattttc
     2581 atcaataatc aatcaagcga ccttacccaa ccttcccttg atggcatcag caccatcaaa
     2641 ctcaaagccg tcaagggaaa taatgaagtt gatttaaatg taaaaaacca cgcctccgta
     2701 aaaggcatta tcgcgtcgca ttctgcaaaa gcaacgcttg aagccgaagg ggacaatatt
     2761 gtctaggtta aaaatccgga caccaatacg gctttaatcg atgctttgaa aaaacaatac
     2821 cccaatgctt cgaaagaact atacagggtg ggggttcaat ccacgactgg aacagtacgc
     2881 atttcgccaa acggcaaaat cgcattaacg gaattgaaaa cttttggaat tgtacaaaac
     2941 ggcatttgcg tca
//
LOCUS       DAWFKN010000138         2468 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFKN010000138 DAWFKN010000000
VERSION     DAWFKN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.138"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(13..819)
                     /locus_tag="WGR46_002091"
     CDS             complement(13..819)
                     /locus_tag="WGR46_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0856939.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="WGR46_002092"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="WGR46_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0856940.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          684 a          622 c          639 g          523 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taagtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattct
//
LOCUS       DAWFKN010000139         2458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFKN010000139 DAWFKN010000000
VERSION     DAWFKN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.139"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            35..1390
                     /locus_tag="WGR46_002093"
     CDS             35..1390
                     /locus_tag="WGR46_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0856941.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WGR46_002094"
     CDS             1394..2404
                     /locus_tag="WGR46_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0856942.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          660 c          740 g          605 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWFKN010000140         2435 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFKN010000140 DAWFKN010000000
VERSION     DAWFKN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.140"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..937
                     /gene="rfbB"
                     /locus_tag="WGR46_002095"
     CDS             <1..937
                     /gene="rfbB"
                     /locus_tag="WGR46_002095"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0856943.1"
                     /translation="NLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHR
                     PDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYWQQMPSEQHEAFRFHHI
                     STDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNN
                     YGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGET
                     YNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTA
                     KIRRDLGWQPLETFESGLRKTVQWYLDNKTRRQNA"
     gene            1043..1909
                     /gene="rfbA"
                     /locus_tag="WGR46_002096"
     CDS             1043..1909
                     /gene="rfbA"
                     /locus_tag="WGR46_002096"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0856944.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1963..>2435
                     /gene="rfbC"
                     /locus_tag="WGR46_002097"
     CDS             1963..>2435
                     /gene="rfbC"
                     /locus_tag="WGR46_002097"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0856945.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIDWPL"
BASE COUNT          619 a          694 c          606 g          516 t
ORIGIN      
        1 caacctcgac aaactgacct acgcgggcaa tctcgaatcg ctgaccgaca ttgccgataa
       61 tccccgctat gctttcgagc aagtggacat ttgcgaccgc gccgaactcg accgcgtatt
      121 cgcacaacac cggcccgatg ccgtgatgca cttggcggcg gaaagccatg tcgaccgctc
      181 tatcggttcg gcaggcgagt ttatccaaac caatatcgtc ggcacattca acctacttga
      241 agcagcccgc gcctactggc aacaaatgcc gtctgaacag cacgaagcct tccgtttcca
      301 ccatatttcc accgatgaag tctatggcga tttaggcggc acggacgatt tgtttaccga
      361 aaccgcgccc tacgcgccgt ccagccctta ctctgcctct aaagcgtcca gcgaccacct
      421 cgtccgcgcg tggttgcgta cttacggctt gccgaccatt gtaaccaact gctccaacaa
      481 ctacggtcct taccattttc cggaaaaact cattcctttg atgattctga acgcgcttga
      541 cggcaaaccg ctgcctgtgt acggcgacgg tatgcaaatc cgcgactggc tgtttgtcga
      601 agaccacgcg cgcgcactgt atcaggttgt taccgaaggt gttgtcggcg aaacctacaa
      661 tatcggcggc cacaatgaaa aagccaatat tgaagtcgtc aaaaccatct gcgccctgct
      721 ggaagaactc gctcccgaaa aaccggccgg cgtggcgcgt tatgaagatt tgattacttt
      781 cgtacaagac cgccctggcc acgatgcccg ctacgccgtc gatacggcga aaatcagacg
      841 ggatttgggc tggcagcctt tggaaacctt tgaatccggc ctccgcaaaa cagtgcaatg
      901 gtacttggac aacaaaactc ggcggcaaaa cgcataaaac ggcagctatc gtttggaacg
      961 tttaggcacc ggaaaataag ttttcagacg gcatcccgac gcaatgccat ctgaaaaccc
     1021 atcgcaaagg aagaaagaaa agatgaaagg catcatactg gcaggcggca gcggcacgcg
     1081 cctctacccc atcacgcgcg gcgtatccaa acagctcctg cccgtgtacg acaaaccgat
     1141 gatttattac cccttgtcgg ttttgatgct ggcaggaatc cgcgatattt tggtgattac
     1201 cgcgcctgaa gacaacgcct ctttcaaacg cctgcttggc gacggcagcg atttcggcat
     1261 ttccatcagt tatgccgtgc aacccagtcc ggacggcttg gcacaggcat ttatcatcgg
     1321 cgaagaattt atcggcaacg acaatgtttg cttggttttg ggcgacaata ttttttacgg
     1381 tcagtcgttt acgcaaacat tgaaacaggc ggcagcgcaa acgcacggcg caaccgtgtt
     1441 tgcttatcag gtcaaaaacc ccgaacgttt cggcgtggtt gaatttaacg aaaacttccg
     1501 cgccgtttcc atcgaagaaa aaccgcaacg gcccaaatcc gattgggcgg taaccggctt
     1561 gtatttctac gacaaccgcg ccgtcgagtt cgccaaacag ctcaaaccgt ccgcacgcgg
     1621 cgaattggaa atttccgacc tcaaccaaat gtatttggaa gacggttcat tgtccgtcca
     1681 aatattagga cgcggtttcg cgtggctgga caccggtacg cacgaaagcc tgcacgaggc
     1741 ggcttcgttc gtccaaaccg tgcaaaacat ccaaaacctg cacatcgcct gcctggaaga
     1801 aatcgcttgg cgcaacggtt ggctttccga tgaaaaactg gaagaattgg cgcgcccgat
     1861 ggcgaaaaac caatacggcc aatatttgct gcgcctgttg aaaaaataat gtttgaggcc
     1921 gtctgaaact tttcagacgg cctttagatg aaagataaaa agatgaacat cattgatacc
     1981 gccattcctg acgtaaaact gcttgagccc caagtcttcg gcgacgcgcg cggctttttt
     2041 atggaaacct tccgcgacga gtggtttaaa acccaagtct gcgaacgcac cttcgtgcag
     2101 gaaaaccact ccaaatccgg caaaggcgta ttgcgcggcc tgcactacca aaccgaaaac
     2161 acacaaggca aactcgtacg cgtagttgtc ggcgaagtgt tcgacgtggc cgtcgatatg
     2221 cgcgaaggtt cgccgacttt cggcaaatgg gcgggcgcaa ccctgtccgc gcaaaaccaa
     2281 tgccagcttt ggatacccga aggcttcgcg cacgggtttt gcgttttggg cgacgcggcg
     2341 gaagtcgtgt acaaatgcac cgactattac aagcctgaag ccgaacaggt tttaatatgg
     2401 aacgacccga cagtcggcat cgactggccg cttca
//
LOCUS       DAWFKN010000141         2298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFKN010000141 DAWFKN010000000
VERSION     DAWFKN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.141"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(27..1436)
                     /gene="dacB"
                     /locus_tag="WGR46_002098"
     CDS             complement(27..1436)
                     /gene="dacB"
                     /locus_tag="WGR46_002098"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0856946.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1601..2167
                     /locus_tag="WGR46_002099"
     CDS             1601..2167
                     /locus_tag="WGR46_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0856947.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          558 c          662 g          550 t
ORIGIN      
        1 aatttaatcc actatatttt cctgtttcag gctcggcgtt ctttgcacat cagtttcgca
       61 tccagccagc catcgccgcc ggagatgatg ttgttggcaa cgaagttgtc caagtcgggc
      121 agcagggaaa cggcgcggcc gctgttgatg atgacgacca ccgccatcgg tttgtcgccc
      181 agccaataac ctgcaagggc gcggacattg ttgagcgtgc cggtttttaa gcgcaacagc
      241 ccgccgcttt gtttgaagcg gttgcgtaaa gtcccgtctg tgccggcgat gggcagcgta
      301 tcgatgaaat cttgtgcaaa cgggctgaaa taagccgttt ccaacatttg cgccatcatt
      361 ctcgccgtta ccctttcttt tctggacaga cctgaaccgt tttccaaaac caaatccgca
      421 acatcgatgc ccgacacggc aagttcacgc cggacggcag acgctgcctg ttcggaaacg
      481 gcgggcagtt tgccgtcgcc gccgagtttg aggaagacgg aacgcgcaat tagattgtcc
      541 gaacgcttgt tcatgtccgt caaaatttcc ttcatcggct ttgagtgtgc aacggcaagc
      601 gtctgcgcgc cttccggcgt gtcggatatg ccgatgccgt ctgaaatccg tccgccgccg
      661 agcagccagt ggttggtaaa actttgccgg atcagttcgt caagcgcgaa catccggaca
      721 ccgacaggct tgcccaaaca gctctcggga atattgccgc gcaatttcag cgtattgtcc
      781 gaaaaagatg cacgcatcag ttttttgatc gaagggcagg cagcttggga ggcggtaatt
      841 ttcaagttgt tttgggcgaa aatatgcggc aaaggcggat cggtgaggat gtcggtactg
      901 tcggcggcat tgcgttcggc gcgcaccata accataccgg cagacagcat agttggattg
      961 gggggcgtca taaacggcga accgctgtcg gcttcgaaat cgtcggggct gccgacttcg
     1021 ccccacaggc tgtggtcgag catcaggtgt cccgtgatat tgcgtatgcc ttgttcgcgc
     1081 aactggcgtt ggacggcaag caggttttcc tgattgaaaa cggggtcgcc gctgcccgcc
     1141 caatacaggt ttccgtcaag cgtgccgtcg tttaccgtac cgttgctttt aaactcggtc
     1201 gcccagcggt aattgctgcc gaaggttttg aaggcggcaa acgcggtaac gagtttcatt
     1261 gtggaggcgg ggttgacggg gacatccgag cggtggtcaa tgatgacttt tccgctgtca
     1321 agctcttgga catatacggc gatttcgttt tgcggaatgc gacctgtatc gagcgcgtgt
     1381 gcggcgaggg aggcgagaag cagcagcagg gaggccgctg ttttggggaa attcataggt
     1441 aaatctgtta cataaaaaaa gcaattataa ggcaaagccg gataagtggg aacgctgtgg
     1501 cggcgggccg gcttgtttgc aggggaaatc acatatataa taatcgctat cattatgaaa
     1561 tgattgagac gcacaaactt aatattcagg aggaatgatt atggctaaga aaatcagtat
     1621 tttggtgggc agcctgcgcc gtgcttcttt tgcgcgcaaa gtggcattga atgcggcgga
     1681 gatgttcccc gaaggctggc aggcggaaat cgtcgaaatc ggacatctgc cgctttacaa
     1741 tttcgattat gacgaccctg cggtggaaga tgtgccgctg cccgaaagct acacggtttt
     1801 ccgcgaaacg attaaggctt cggacggcat tttgttcgtt acgtccgaaa ataaccgcac
     1861 cattcccgcc tgtttgaaaa atgcggtgga catcggctcg aaaccgaatg ccgacgtggc
     1921 gtggaaaaac aaaccggccg gcatcatcag ccattccgtc ggcaagatgg gcggttacag
     1981 ttcgcaaaaa aacctgcgcc ttgcgctgtc ttatttcgat atgcccgtaa ccggacagcc
     2041 ggaagtgttt ttgggcaatt cgccgacact gtttgatgaa aacggcaaat tgatcgactc
     2101 ggcaagggat tttgttcagt catatattaa tcagtttgtc ggtttgattg aaagaaatgc
     2161 caaataaaca aacaagtcga aaggccgcaa aaccggttcg ggttttgcgg cttttttata
     2221 tgggctgggc agcgtcaagg tttgccgttt gccggataat gcgcggcaaa tgaccggcgt
     2281 gtttatagcg gattaaca
//
LOCUS       DAWFKN010000142         2208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFKN010000142 DAWFKN010000000
VERSION     DAWFKN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.142"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            345..2123
                     /locus_tag="WGR46_002100"
     CDS             345..2123
                     /locus_tag="WGR46_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0856948.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     AGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          670 a          538 c          558 g          442 t
ORIGIN      
        1 cacaggaatc tatcagaaaa aacagaaacc cccaccgccg tcattcccgc gcaggcggga
       61 atccagcaac acaacgcggc aggactttat cggaaaaaac agaaacccca ccgaccgtca
      121 ttcccgcaaa agcgggaatc cagacccgtc ggcacggaat cttaccggat aaaacagttt
      181 ccttagattc cacgtcccag attcccgcct tcgcgggaat gacgagattt taagttgggg
      241 gaatttatca gaaaaccccc aacccccaaa aaccgggcgg atgccgcacc atccgccccc
      301 aaaccccgat ttaaccattc aaacaaacca aaagaaaaaa caaaatgaac aaaatatacc
      361 gcatcatttg gaatagtgcc ctcaatgcct gggtcgccgt atccgagctc acacgcaacc
      421 acaccaaacg cgcctccgca accgtgaaga ccgccgtatt ggcgacactg ttgtttgcaa
      481 cggttcaggc gaatgctacc gatgaagatg aagaagaaga gttagaatcc gtacaacgct
      541 ctgtcgtagg gagcattcaa gccagtatgg aaggcagcgg cgaattggaa acgatatcat
      601 tatcaatgac taacgacagc aaggaatttg tagacccata catagtagtt accctcaaag
      661 ccggcgacaa cctgaaaatc aaacaaaaca ccaatgaaaa caccaatgcc agtagcttca
      721 cctactcgct gaaaaaagac ctcacaggcc tgatcaatgt tgaaactgaa aaattatcgt
      781 ttggcgcaaa cggcaagaaa gtcaacatca taagcgacac caaaggcttg aatttcgcga
      841 aagaaacggc tgggacgaac ggcgacacca cggttcatct gaacggtatc ggttcgactt
      901 tgaccgatac gcttgcgggt tcttctgctt ctcacgttga tgcgggtaac caaagtacac
      961 attacactcg tgcagcaagt attaaggatg tgttgaatgc gggttggaat attaagggtg
     1021 ttaaaactgg ctcaacaact ggtcaatcag aaaatgtcga tttcgtccgc acttacgaca
     1081 cagtcgagtt cttgagcgca gatacgaaaa caacgactgt taatgtggaa agcaaagaca
     1141 acggcaagag aaccgaagtt aaaatcggtg cgaagacttc tgttattaaa gaaaaagacg
     1201 gtaagttggt tactggtaaa ggcaaaggcg agaatggttc ttctacagac gaaggcgaag
     1261 gcttagtgac tgcaaaagaa gtgattgatg cagtaaacaa ggctggttgg agaatgaaaa
     1321 caacaaccgc taatggtcaa acaggtcaag ctgacaagtt tgaaaccgtt acatcaggca
     1381 caaatgtaac ctttgctagt ggtaaaggta caactgcgac tgtaagtaaa gatgatcaag
     1441 gcaacatcac tgttatgtat gatgtaaatg tcggcgatgc cctaaacgtc aatcagctgc
     1501 aaaacagcgg ttggaatttg gattccaaag cggttgcagg ttcttcgggc aaagtcatca
     1561 gcggcaatgt ttcgccgagc aagggaaaga tggatgaaac cgtcaacatt aatgccggca
     1621 acaacatcga gattagccgc aacggtaaaa atatcgacat cgccacttcg atggcgccgc
     1681 agttttccag cgtttcgctc ggcgcggggg cagatgcgcc cactttaagc gtggatgacg
     1741 agggcgcgtt gaatgtcggc agcaaggatg ccaacaaacc cgtccgcatt accaatgtcg
     1801 ccccgggcgt taaagagggg gatgttacaa acgtcgcaca acttaaaggc gtggcgcaaa
     1861 acttgaacaa ccgcatcgac aatgtggacg gcaacgcgcg tgcgggcatc gcccaagcga
     1921 ttgcaaccgc aggtctggtt caggcgtatc tgcccggcaa gagtatgatg gcgatcggcg
     1981 gcggcactta tcgcggcgaa gccggttacg ccatcggcta ctccagtatt tccgacggcg
     2041 gaaattggat tatcaaaggc acggcttccg gcaattcgcg cggccatttc ggtgcttccg
     2101 catctgtcgg ttatcagtgg taagggcttt ttcgcctgtc tgctgttggg acaggcggaa
     2161 ggtttgaagg gaagggtggc gatttgccgc ctgagacctt tgcaaaat
//
LOCUS       DAWFKN010000143         2150 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFKN010000143 DAWFKN010000000
VERSION     DAWFKN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.143"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            30..463
                     /locus_tag="WGR46_002101"
                     /pseudo
     CDS             30..463
                     /locus_tag="WGR46_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            559..1182
                     /gene="rsmG"
                     /locus_tag="WGR46_002102"
     CDS             559..1182
                     /gene="rsmG"
                     /locus_tag="WGR46_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0856949.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1281..2054
                     /locus_tag="WGR46_002103"
     CDS             1281..2054
                     /locus_tag="WGR46_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0856950.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          423 a          490 c          739 g          498 t
ORIGIN      
        1 gcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 ttgcgcttgc gttcggacgg gcggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgtggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      241 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      301 gtggattttc gagctgctgt ttggcagtgc cgccttgggc gggctgcgtg gggaggcggt
      361 gttggaatat gcgtttgccg tgtggctggt gtcgatgctg acactgccca aacgcttgac
      421 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggacggaa cgggggatgc
      481 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgttaacat tacgcctgaa
      541 catggacagg atagggatat ggaacgcaaa gaacgcctgc gtgcaggcat cgccgcattg
      601 ggtttggata tttccgcagc ggcgcaggac aggcttttgg cctatgtgga tttgttgaaa
      661 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat ggttgtccat
      721 catcttttgg acagcctgac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      781 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      841 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      901 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
      961 gccgacgtgg ttaccagccg tgcgtttgca gaactggcgg actttgtatc gtggacggcg
     1021 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc gcagggggaa
     1081 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccaggc
     1141 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt cagacggcat
     1201 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1261 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1321 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1381 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1441 aggcgagttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgaaatcgg
     1501 cggcggtacg cagcaaagag ggcggatacg gcgtgttggg tgcgaaccgc gcgctggccg
     1561 gcgcggaaat cgagctggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1621 aggcagtggc ggaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1681 tgacgcttaa cggcttggtg gcggcaggcg gcgtgattgt gccgatgttg tgcgaatatt
     1741 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1801 atcccgattt ggatatcacg ggcatcgtgc gtacgatgta cgacagccgc agcaggctag
     1861 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgctgttt gaaaccgtca
     1921 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttatg
     1981 atgcgcaggc aaagggtgcc aaggcgtatc ttgccttggc ggacgagctg atggcgaggg
     2041 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2101 tgcgttaata tggcggatta aaataaaaat acttatatcg tcatttatag
//
LOCUS       DAWFKN010000144         2139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFKN010000144 DAWFKN010000000
VERSION     DAWFKN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.144"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..291
                     /locus_tag="WGR46_002104"
     CDS             <1..291
                     /locus_tag="WGR46_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0856951.1"
                     /translation="EAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDN
                     YSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            308..>2139
                     /gene="frpC"
                     /locus_tag="WGR46_002105"
     CDS             308..>2139
                     /gene="frpC"
                     /locus_tag="WGR46_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ0856952.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQ
                     VVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDG
                     HQCLAPWVKETKKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATG
                     WVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINA
                     ADAAFQSLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGS
                     YTKTNGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIG"
BASE COUNT          703 a          399 c          495 g          542 t
ORIGIN      
        1 gaggcttatg aagataaaag acatattcct gaaaatccat attttcatga attttactat
       61 attaaaaaag gagaaaatcc ggcgattatt actcattgga ataatcgagt aaaccaggct
      121 gaagaagata attatagcac tagcgtaggt tcctgtatta acggtttcac ggtacagtat
      181 tacccgttta ttcgggaaaa gcagcagctc acacagcagg agttggtagg ttatcaccaa
      241 caagtagagc aattggtaca gagttttgta aacaattcaa gtaaaaaata atttaaagga
      301 tcttattatg aatgagggtg aagttgtttt aacaccagaa caaatccaaa ccttgcgtgg
      361 ttatgcttcc cgtggcgata cctatggcgg ttggcgttat ttggctaatt tgggtgaccg
      421 ttatgcggat gatgctgctg caattgtcgg taaggatgca aacttaaatg gtttgaattt
      481 atggatgaaa aaaggtgtgg aaaacctatg ggatgatacg gtcggtaaaa agacccgttt
      541 agagaaattt gatcgggttg cactgcaaca tttcaggcaa tatgcgcgtc taattaatca
      601 aaataatggt agattaccca atactagtga aattgagaga agttactata aagccgttac
      661 cgataatggc gtttcttcca gtgcagctat tgatttagtt attaatcgtt cacttccgga
      721 tatggcggat ggttattggg cattaggttt ggggatagaa gccgaacgta tccacaatga
      781 gcaagcagta aataatccga acggtagcga aagggataat agaaagcagt taatatctgc
      841 tttagataaa ggatttgatg gatcttttaa agagaagcat tttacttttt tacaatctgt
      901 gatgatggat gtaacaaagt taggtgttga atatacaata gatggttggc aaaaaattgg
      961 aggttggggt aatgggataa tcaatgattt atataaaagt gttgtaaaaa gagagtggac
     1021 tggaatattt gagatcgtta ataataacat caagcaagga aatgaagctt ttaaaaatga
     1081 aatcaatagc ttggttcatg atatgaaagc tgctggcaag gaatttggag atgacttaaa
     1141 tacacagtgg aataatctca ctcaggctgc cgaaataatc tataatgaca tagtagacaa
     1201 tactagtcaa ggaatagaaa aaggtgtcaa agccattaaa gaattgtctg aaaaaatgaa
     1261 aaatgctgct tccgatttgg ctgacggttc agcagagaaa gctaaacaag tagtggaaga
     1321 tttggctcaa gccgccaaag aagcatacga aaatgccaaa tccacagccg agaaggctgc
     1381 tcaagcagct cgagaatttt ttaagggctt gcccagtttt aaagatctgg ccgaaaaatt
     1441 tagagatctg ttcccaaatc cggaaggctg gatcgatgat ggtcaccaat gtttagctcc
     1501 ttgggttaaa gaaactaaaa aacgcaatgg caaatatcat gtctacgacc cccttgccct
     1561 agacctagat ggcgacggta tagaaaccgt tgccaccaaa ggctttgcag gcagcttatt
     1621 tgatcacacc aacaacggta tccgcaccgc caccggttgg gtttctgccg atgacggttt
     1681 actcgtccgc gatttgaacg gcaacggcat catcgacaac ggtgcggaac tcttcggcga
     1741 caacaccaaa ctggcagacg gctcttttgc caaacacggc tacgcggctt tggccgaatt
     1801 ggattcaaac ggcgacaaca tcatcaacgc ggcagacgcc gcattccaat ccctgcgtgt
     1861 atggcaggat ctcaaccagg acggcatttc ccaaaccaac gaactccgca cccttgaaga
     1921 attgggtatt caatctttgg atctcgccta taaagatgta aataaaaatc tcggtaacgg
     1981 taacactttg gctcagcaag gcagctacac caaaacaaac ggtacaaccg caaaaatggg
     2041 ggatttactt ttagcagccg acaacctgca cagccgcttc aaagacaaag tggaactcac
     2101 tgccgaacag gcaaaagccg ccaatcttgc gggcattgg
//
LOCUS       DAWFKN010000145         2114 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFKN010000145 DAWFKN010000000
VERSION     DAWFKN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.145"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            413..1870
                     /locus_tag="WGR46_002106"
     CDS             413..1870
                     /locus_tag="WGR46_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0856953.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          485 a          619 c          487 g          523 t
ORIGIN      
        1 gacggtcttt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
       61 aaacagtacg gaaccaattc acttggtgct tcagcacctt agagaatcgt tctctttgag
      121 ctaaggcgag gcaacgccgt actggttttt gttaatccac tataaaaaac cgccctatcg
      181 gataagtcgc ttgactgaaa aggctttacc cgcgccgtat cggaacacgt cctctaaaat
      241 acaatccgtt gaattgaaac aaatataaaa acattcgacc gcaaaaacgg cagcgcatcg
      301 tttgacaaag attgaaaata tcggttaaaa accgattttc atacaaaaaa caccgctgcc
      361 gtccgcatcc gtttcagacg gtattgagag aaaatctttt aggagaacct ttatgtcccg
      421 gcatcccgcc cgcaccggag aaaaaacatt cttcggccac cccttccagc tttccaccct
      481 cttccatatc gaattgtggg aacgtttttc attttacgga atgcagggca tcctgctgat
      541 ttacctctac tacaccgccg acaaaggcgg cttgggcata gacaaaaccc tcgccggcgg
      601 cattgtcggc gcatacagcg gcagcgtgta cctgtccacc attttgggcg cgtggtttgc
      661 cgaccgcgta tggggtgcgg aaaagaccct cttcctctcg ggcatcgtcg tgatgctcgg
      721 acacatcgtc cttgccgccg ccccgggcct gtacggcctt ttaatcgggc tgatattcat
      781 cgcattgggc agcggcggtg tgaaatctac ggccagttct atggtgggcg cattatacga
      841 acaggacgaa atgcgcccgc tgcgcgatgc gggattttcc attttctaca tcgccatcaa
      901 tatcggcggc ttcctaggcc cgctgctgac cggcctgctg caggaaaaca tcggtttcca
      961 ttatggtttc ggcgcggcgg cggtcggtat ggcattcggc ttgtggcgtt attccttggg
     1021 acgtaaaaac ctgccccacc ccaccgtccc ccatccgctt tcaaaaggac agggcaaaac
     1081 tgcggccgcc gtcggcatcg ccctcatcgc cgcacttgca accgccatca aaaccgggct
     1141 tgtcaacctc gacaatttct ccggcatcct cctatctacc gtcatccttg ccgtcatcgt
     1201 ctattttacc cggctgctga ccaacccccg cgtcagttcc gacaacaagc ggcacatcat
     1261 cgcctacatc ccgcttttcc tgaccatctg tatgttttgg gcggtctggt ttcagattta
     1321 caccgtggca accgtctatt tcgacgaaac cgtcaaccgc accatcggtt cgtttaccgt
     1381 acccgtcgct tggaaagatt ctatgcaaag cctgtgggtc atcctgtttt ccggacttat
     1441 ggcggcaatg tggacaaaaa tggggcgcaa acagcccaaa accccgctga aattcgctat
     1501 ggcggtattt gttaccggcg cgtcgttttt gggattcgtc ccctttattt ccgccggcac
     1561 gccgatgcct attgcggttt tcgcactgat tgtccttgcc atcacgatag gcgaactgat
     1621 gatttccccg attgcgctgt ccatctccac caaaatcgca ccgcctttat tcaaaaccca
     1681 aatggtcgcc cttaatttcc ttgccttttc attaggcttc actttgggcg gcgtattgtt
     1741 tgaaaaaggc tatcaggcgg gcgacgaaat cggcttctat cggctgctgt tctacatcgg
     1801 cgcagccaca ggcttcctgc tgctcctgct cgtccccaaa ttgaacaaaa tgctcgaagg
     1861 cacagactaa gtcccgcccc gatgccgtct aaacccttca gacggcattt ttccgcataa
     1921 tgaaaccaaa ccgtttccac ccgacaggac aggctccccc ccaaccggaa ggcagcctgc
     1981 cgattgtcat ttaaatatct gactgattcc gtctgtttgc aagggaaata gccgtttatt
     2041 tccgcttggg cggaaaccgg ttctattgag taaaaggcat tttatccgac taaattagtg
     2101 ctgcatcaac ggaa
//
LOCUS       DAWFKN010000146         2099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFKN010000146 DAWFKN010000000
VERSION     DAWFKN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.146"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..1443
                     /locus_tag="WGR46_002107"
     CDS             <1..1443
                     /locus_tag="WGR46_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0856954.1"
                     /translation="INGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYITHKIA
                     HAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            1448..1831
                     /locus_tag="WGR46_002108"
     CDS             1448..1831
                     /locus_tag="WGR46_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856955.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
BASE COUNT          719 a          391 c          429 g          560 t
ORIGIN      
        1 atcaacggcg gcagcctcaa agacaacttg ggcgatgccg cactgggtgc gatagtcagt
       61 accgtacacg gagaagtagc gagcaaaatc aaatttaatc tcagcgaaga ctacattacc
      121 cacaagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggtaagtgt
      181 caggatggtg cgatcggtgc ggctgtgggc gagatagtcg gggaggcttt gacaaacggc
      241 aaaaatcctg ccactttgac agctaaagaa cgcgaacaga ttttggcata cagcaaactg
      301 gttgccggta cggtaagcgg tgtggtcggc ggcgatgtga atacagcggc gaatgcggct
      361 aaagtcgcga ttgaaaataa cctattatct caagaagagt atgctcttag agaaaaattg
      421 atcaaaaaag ccaaagggaa aggcctatta tctttagatt ggggcagcct gaccgaacaa
      481 gaggcaaggc agtttatcta tttgattgag aaagatcgat attctaatca attgcttgac
      541 cgatatcaaa aaaatccaag tagtttaaat aatcaagaaa aaaatattct tgcatatttt
      601 attaaccaaa cctctggagg taacacagct tgggcagctt cgatactgaa aacgccccag
      661 tcaatgggta atctcactat tccttccaaa gatattaata acaccttatc gaaagcctat
      721 caaacattga gtcgttatga ttcttttgat tacaaatcag ctgttgccgc acaacctgca
      781 ctttacttat taaacggacc gcttggcttc agtgtcaaag cagctactgt ggcagcagga
      841 ggatataaca ttggacaggg agcgaaagca atctctaatg gagaatatct gcatggtaca
      901 gttcaggttg ttaatggcac attgatggtt gcaggatctg tatctgcaca ggctgcaata
      961 tcggccaagc ctgcacctgt tacccgttat ctgagcaatg acagtgctcc tgctttaaga
     1021 caagctttaa ctgctgaaag ccagagaatc cgcatgaaac tgccggaaga gtatcgacaa
     1081 atagggaatc ttgcgatagc aaaaattgat gttaaaggat taccgcaaag gatggaagca
     1141 tttagttctt tccaaaaagg ggaacatgga tttatttcgt tacctgaaac aaaaattttt
     1201 aaacctatat ctgttgataa atatcataat attgcctctc ctcctagagg aacattaaga
     1261 aatatagatg gagaatataa attacttgaa actatagcac agcaactcgg aaataatcgt
     1321 aatgtatcag gtagaattga tctatttaca gaattaaagg cctgtcaatc ttgcagcaat
     1381 gttattttag agtttagaaa tcgctatcca aatattcaat taaatatttt tacaggaaaa
     1441 tagaatcatg gaaaaaataa aaaattatat agaattcaag aaattattta aaaatataac
     1501 ggaaaaattt gtcagagatt tagatataac attaattgaa gcagctgaat attctataat
     1561 tgaattcgaa atttatttat ctgaatatcc ggaagatagt acatttattt ttacagcatg
     1621 ggcaatctat ctgttagaaa ataactgtct caatgaaaga ggaaaagata attttttcat
     1681 cgcggaaatg aaaaaatgtt atcaacaaac taatttggat agaataaaat ctatctattc
     1741 ttcgagtgac tatcatattt ttgctaagga tgtaaacaag attcaacaaa catttaaatt
     1801 aaatcttgcc aatatcatgc agcaagagta gcaactgtat ttttaacccc gtcgggcaaa
     1861 attaccaaaa acggtaaatc actaaaaata cttttatacc gccgccgcag caagctgtag
     1921 cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctc cagcacgcac gcgttccatg
     1981 atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt tgttatgcaa
     2041 gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga cacccccta
//
LOCUS       DAWFKN010000147         2051 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFKN010000147 DAWFKN010000000
VERSION     DAWFKN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.147"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(264..695)
                     /locus_tag="WGR46_002109"
     CDS             complement(264..695)
                     /locus_tag="WGR46_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856956.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(711..>2051)
                     /locus_tag="WGR46_002110"
     CDS             complement(711..>2051)
                     /locus_tag="WGR46_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0856957.1"
                     /translation="VNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAH
                     AIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAG
                     TVSGVVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKY
                     QNVADKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFS
                     KSYTQADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFV
                     KQNTPITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVK
                     SETQTDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAA
                     SQGYSKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          544 a          409 c          377 g          721 t
ORIGIN      
        1 gaactgatat ccgtggtgct gagcaggatg cgactgttct cattggcata cggtgcgcta
       61 atgctcacac cggttttacc cgaaagttct gcagcaagcg tagggggtgt cttcagcacg
      121 cacgcgtctg ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg
      181 aaaaattgtg agcttttcag acggcattga gccgtaaatc atggaacgcg tgcgtgctga
      241 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagtttgt ttgagaatat
      301 gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa gaaatcagat
      361 attgcaatat taaagtaatg atatgctaaa ttaataggta taattaattc ttcaggattg
      421 aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc aaatccattt
      481 acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg agcacctatt
      541 actccaaaat gtccggtagt tgtaatattt ttaaaacact ctaaaagcgt atctttagtt
      601 tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt atttatagat
      661 tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa ttattctggg
      721 tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt gattccgtca
      781 aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt tctttcattt
      841 tgagcaattt tagaggcttt tgaatatcct tgtgaagcag cattttgagc catttgaagt
      901 attttatcat cagaaaattt tttaggatta taaacagttt ttatacttga aatttcctta
      961 aatccaccat caggtttacc tgtcctgtct agtgtaggaa tctcatattt aattcgggta
     1021 atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga atttagttct
     1081 gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact aaaaccatca
     1141 gcatttgcca gatgtctttt taggtttgta tcgaaactga tgccttccgg gtatttgaca
     1201 ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa tctaggatta
     1261 actcctgaaa taagtgtata accttggtcg gacatccatt cggataaagg ctttgtattg
     1321 cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa agccaaatct
     1381 gcttgggtgt aagatttgct gaataattta taccattcat catctttacc aattagacct
     1441 gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct gattgttcta
     1501 cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag tcttttatca
     1561 gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt attctgtttg
     1621 gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag ctgattattt
     1681 ttcaccgcta cctcagccgc attcgccgcc gcatttacat cgccgccgac cacaccgctt
     1741 accgtaccgg caaccagttt gctgtatgcc aaaatctgtt cgcgttcttt agctgtcaaa
     1801 gtgtcaggat ttttgccgtt tgtcaaagcc tccccgacta tctcgcccac agccgcacct
     1861 atcgcaccat cctgacactt gcccttattc gccgccgctg ccgcacagcc cgctatggca
     1921 tgggcaatct tgtggactat gtagtgctga tccaactgtt tgattttact ggctgcttct
     1981 ccatgcgcgg tattgaccaa agccgcaagg atattcgctt ccagattgtc tttcaggctg
     2041 ccgccgttga c
//
LOCUS       DAWFKN010000148         1991 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFKN010000148 DAWFKN010000000
VERSION     DAWFKN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.148"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            117..389
                     /locus_tag="WGR46_002111"
     CDS             117..389
                     /locus_tag="WGR46_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856958.1"
                     /translation="MDKAKLFELIEPSHHKLPKSLTPEAYDCAFLKAQAIHEQPAFKP
                     YKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            434..724
                     /locus_tag="WGR46_002112"
     CDS             434..724
                     /locus_tag="WGR46_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856959.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            729..926
                     /locus_tag="WGR46_002113"
     CDS             729..926
                     /locus_tag="WGR46_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856960.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            934..1218
                     /locus_tag="WGR46_002114"
     CDS             934..1218
                     /locus_tag="WGR46_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856961.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEVGWIVVGIFAALFVFSIVKRVMK"
     gene            1225..1503
                     /locus_tag="WGR46_002115"
     CDS             1225..1503
                     /locus_tag="WGR46_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856962.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGVMASVYFVSLLKKVLK"
     gene            1631..1948
                     /locus_tag="WGR46_002116"
     CDS             1631..1948
                     /locus_tag="WGR46_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0856963.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          616 a          418 c          431 g          526 t
ORIGIN      
        1 gcaacgcgca tacacgcggt taaggaagat aaggtcattt cagccgaccg ttaccttgaa
       61 tgggtaaaaa acagttcgga cgtgcggcaa acggtctgaa attcattttt cccgaattgg
      121 acaaagccaa actgtttgaa ctgattgagc cgagtcatca caagctgccc aagtctttga
      181 ctcccgaagc ctacgactgc gcctttttga aagctcaagc cattcatgaa cagcccgcat
      241 tcaaaccgta caaagaccct tactatatgt acgaatatta cgagaatctt gaaaaacagc
      301 ttgaacagca aaaacacgtc aacaatgaag aaagctataa caacttcatt tacgacaaat
      361 tcgcaagact accgatttca tgggcttaaa gtgtctgccc gaaagacgtt taatcacaca
      421 aggaaaccaa aaaatgaaca tccaacttca aggccacatc gtcggcgtta aaaaattcaa
      481 cggacaaatc gaaggcaaga gcttcgacta ttgccgcctg attgtcgcca cacccttaga
      541 cagctcccaa ggcaacgcat tgggcagctc tactactgaa tacgatttcg gcggctctgc
      601 caatttcgag cagttccgaa acgcccaatt tcccatcgaa gcaaacctga acgtggaaat
      661 cgttactacg ggcaaaaccc aaaaactgaa agtcatcggt tttcaactcg tgaagaaagg
      721 ctgattgaat gcagaaagtc tatgttgtcc agtccgtatc aacaggggac tttctgtatc
      781 tctctcctga aacgggcgac atcggacata ccaaattaat caccaatgcc gattatttct
      841 acgacttcga agaagcgatt aacgcaggtt tggaagaaat cggcaaccaa tacgaatttg
      901 tcgtattcgg atttttgaaa gactgatttt cggatgttcg gcggtcgtct gaaaaacgct
      961 ccatccatta ccgccaaaca ctttttgaag gaaaatatca tgaaatttat taacacctgc
     1021 cgtaaatacg gcgcaaaact ggctgttgta acagctgctc ccctggcttt ggccgcacat
     1081 gcaaatgcaa cgttgcccga tacagcaaaa aacgctttgg aagccgcaaa agccgacggt
     1141 atggaagtcg gttggattgt agtgggcatt ttcgccgcgc tttttgtctt ttccatcgtt
     1201 aagagagtga tgaagtaaga cggcatgtac taccaagtcg gaaataaatg tcttgagaag
     1261 caccaggctg aaaaccttta tttcagcttg gtagtaccaa gaatcaaaga aaacggacag
     1321 attgtcaggc cggaatataa cggcagcctg tggaagatgt cggacggtca gccgctaagg
     1381 cttttattgg cggaatgcag tctgaaagac aacctgcaaa gcggccttga aacaggctgg
     1441 atagtattcg gcgtgatggc gtccgtttac tttgtttccc tgctgaaaaa ggttttgaaa
     1501 tgatggattt ttatttttat ctcggcgttt ccgtacccgt attaatcggg gcggttctgt
     1561 ttaagaattg agcgcatgaa gttatggtgt caaaatcagg ctttcaaaac aatcattgaa
     1621 aggcagaacc atgaacaagc cgtttatcac tcaggcgcag ttggcacttt ataaatatca
     1681 gccgtctagc aagtattttg ggcaatcgat ggcggttata gcgcaatctg aatttgttga
     1741 atttgcgaag attaataagt ctgaaaatgt tattgattgt ttctcttttt tctggaatag
     1801 aagaattaaa catgatattt ggctaatctc attttctgat aattcagaaa tggtaattaa
     1861 agaatccctg aaagatggtc ataaaatata caaatttgaa ttttgcgaaa ttgtcgataa
     1921 ttgcaatttt gatgatgtat tcgtttgaag cgaatgcaaa tgcagtaaaa atatctgaaa
     1981 ctgtttcagt t
//
LOCUS       DAWFKN010000149         1944 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFKN010000149 DAWFKN010000000
VERSION     DAWFKN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.149"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            294..1481
                     /locus_tag="WGR46_002117"
     CDS             294..1481
                     /locus_tag="WGR46_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0856964.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGAGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVS
                     VAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFK
                     RHDDMSVSVRYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQRPTLVPAVVGK
                     PGSDVYYAGLNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDQAKGTDPLKNHQ
                     VHRLTGGYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGF
                     DLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLR
                     HKF"
BASE COUNT          498 a          444 c          504 g          498 t
ORIGIN      
        1 gccgcgtgtg ttttttatgg cgttttaaaa agccgggact gcatccgggc agcagcgcat
       61 cggcccgcac gaggtctgcg cttgaattgt gttgtagaaa cacaacggtt tttgaaaaaa
      121 taagctattg ttttatatca aaatataatc atttttaaaa taaaggttgc ggcatttatc
      181 agatatttgt tctgaaaaat ggttttttgc gggggggggg gtataattga agacgtatcg
      241 ggtgtttgcc cgatgttttt aggtttttat caaatttaca aaaggaagcc gatatgcgaa
      301 aaaaacttac cgccctcgta ttgtccgcac tgccgcttgc ggccgttgcc gatgtcagcc
      361 tgtacggcga aatcaaagcc ggcgtggaag gcaggaacta ccagctgcaa ttgactgaag
      421 cacaagccgc taacggtgga gcgggagcga gcggtcaggt aaaagttact aaagttacta
      481 aggccaaaag ccgcatcagg acgaaaatca gcgatttcgg ctcgtttatc ggctttaagg
      541 ggagcgagga tttgggcgaa gggctgaagg ctgtttggca gcttgagcaa gacgtatccg
      601 ttgccggcgg cggcgcgacc cagtggggca acagggaatc ctttatcggc ttggcaggcg
      661 aattcggtac gctgcgcgcc ggtcgcgttg cgaatcagtt tgacgatgcc agccaagcca
      721 ttgatccttg ggacagcaat aatgatgtgg cttcgcaatt gggtattttc aaacgccacg
      781 acgatatgtc ggtttccgta cgctacgatt cccccgaatt ttccggtttt agcggcagcg
      841 tccaattcgt tccggcccaa aacagcaagt ccgcctatac gccggctcat tttgttcaga
      901 ataagcaaaa tcagcggcct actctcgttc cggctgttgt cggcaagccg gggtcggatg
      961 tgtattatgc cggtctgaat tacaaaaatg gcggttttgc cgggaactat gcctttaaat
     1021 acgcgaaaca cgccaatgtg ggccgtgatg cttttgagtt gttcttgctc ggcagcggga
     1081 gtgatcaagc caaaggtacc gatcccttga aaaaccatca ggtacaccgc ctgacgggcg
     1141 gctatgagga aggcggcttg aatctcgcct tggcggccca gttggatttg tctgaaaatg
     1201 ccgacaaaac caaaaacagt acgaccgaaa ttgccgccac tgcttcctac cgcttcggta
     1261 atgcagttcc acgcatcagc tatgcccatg gtttcgactt gatcgaacgc ggtaaaaaag
     1321 gcgaaaatac cagctacgat caaatcatcg ccggcgttga ttatgatttt tccaaacgca
     1381 cttccgccat cgtgtctggc gcttggctga aacgcaatac cggcatcggc aactacactc
     1441 aaattaatgc cgcctccgtc ggtttgcgcc acaaattcta aatagcgggg cggtgaagcg
     1501 gatagctttg tttttgacgg cttgccttca ttctttgatt gcaatctgac tgccaatctg
     1561 cttcagcccc aaacaaaaat ccggatacgg aagaaaaacg gcaataaaga cagcaaatac
     1621 cgtctgaaag attttcagac ggtatttcgc atttttggct tggtttgcac atatagtggg
     1681 ggactgttag tttttgcaca aggaacaaat agagtaaaaa aacgctgaaa tctcggaaag
     1741 acgtggattt cggcgttttt ttgcatccgg aaaagttacg ccagcttttt cacgaaatcg
     1801 cgccggaatg cgcggttttc tgtttaaagc tgacgagatt agggaatttt taaaactgtt
     1861 ttaagaggtt tttaaaatgg atttaatcaa tactccggcc ataccactca acctgtgttg
     1921 ccgctttgac gaactgagca tccc
//
LOCUS       DAWFKN010000150         1935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFKN010000150 DAWFKN010000000
VERSION     DAWFKN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.150"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            389..1807
                     /locus_tag="WGR46_002118"
     CDS             389..1807
                     /locus_tag="WGR46_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0856965.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT          419 a          622 c          450 g          444 t
ORIGIN      
        1 aaattataac ccccttcctg ccgacgcacg cactttccgc gcggcgcatt ccccttttcc
       61 cgcccctcaa atccgccttt tcttcaggca gggtttcagc ccgcctcttt tccctgtttt
      121 cctttccccg acacgcgtgc gcgccccctg ccgcactgtg ctgcactttc gcgcccggac
      181 ggcatcgttc cgccatccgg ttctctgttt taaatacccc tgtttcagaa agaaatgcag
      241 atgtttcaac acacaggacg acacataaag caccgcccta tgtgttgccc tgatttggaa
      301 ggggttacgc ctcccaaata aagtctgatc ctgccgcccc gaaggacaga tgtccgagtg
      361 gcgaagtttc aaccgaaaag gaaatacgat gaatattcac accctgctct ccaaacaatg
      421 gacgctgccg ccattcctgc cgaaacggct gctgctgtcc ctgctgatac tgctggcccc
      481 caatgcaatg ttttgggttt tggcactgct gaccgccacc gcccgcccga ttgtcaattt
      541 ggactatctt cccgccgcgc tgctgatcgc cctgccttgg cgtttcgtca aaattgccgg
      601 cgtattggcg ttttggctgg cggttttgtt tgacgggctg atgatggtga tccagctctt
      661 cccttttatg gacctcatcg gcgccatcaa cctcgtcccc ttcatcctga ccgcccccgc
      721 cccttatcag ataatgaccg ggctgttgct gctgtatatg ctggcgatgc cgtttgtgtt
      781 gcagaaagcc gccgccaaaa ccgacttccg acacattgcc gcctgtgccg ccgttgtggc
      841 ggcagccggc tatttcactg gccatttgag ttactacgac cgggggcgga tggccaatat
      901 cttcggcgca aacaacttct actacgccaa aagtcaggcg atgctctaca ccgtcagcca
      961 gaatgccgac tttattaccg ccggcctggt cgatcccgtc ttcctcccct tgggcaatca
     1021 acagcgtgcc gccacgcatc tgaacgagcc gaaatctcaa aaaatcctct ttatcgtcgc
     1081 cgaatcttgg gggctgccgg ccaatcccga acttcaaaac gccacttttg ccaaactgct
     1141 ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt tttcccttta tcggcgcgac
     1201 ggtcgaaggc gaaatgcgcg aactgtgtgc ctacggcggt ttgcgcgggt tcgcactgcg
     1261 ccgcgcgccc gacgaaaaat ttgcccgctg cctccccaac cgtttgaaac aagaaggtta
     1321 cgccaccttt gcgatgcacg gcgcgggcag ttcgctttac gaccgcttca gctggtatcc
     1381 gagggcgggc tttcaagaaa tcaaaaccgc cgaaaacctg atcggtaaaa aaacctgcgc
     1441 cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa gtgtcggcat ttttcaaaaa
     1501 acacgacaag ggactgtttt actggatgac gctgaccagc cacgccgact atcccgaatc
     1561 cgacattttc aaccacaggc tcaaatgcac cgaatacggc ctgcccgccg aaaccgacct
     1621 ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa ctggcggatt tgatccaacg
     1681 ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac catccgccgc ccgtcggcaa
     1741 cctcaatgaa accttccgct acctcaaaca ggggcacgtc gcctggctgc acttcaaaat
     1801 caaataacaa caatgccgtc tgaacgcacc aacagccttc agacggcatt ttgcagacag
     1861 accgaccctt caagcccact tttttcatca tctccgataa attgctttgt atagtggatt
     1921 aacaaaaacc agtac
//
LOCUS       DAWFKN010000151         1932 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFKN010000151 DAWFKN010000000
VERSION     DAWFKN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.151"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..354
                     /locus_tag="WGR46_002119"
     CDS             <1..354
                     /locus_tag="WGR46_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856966.1"
                     /translation="HLLPLLEGIAEGTTVYADKGYDSAENRQHLEERQLLDGIMRKAH
                     RNRPLTEVQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCL
                     NLLKAANRLSAPAAA"
     gene            502..681
                     /locus_tag="WGR46_002120"
                     /pseudo
     CDS             502..681
                     /locus_tag="WGR46_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(678..1184)
                     /locus_tag="WGR46_002121"
     CDS             complement(678..1184)
                     /locus_tag="WGR46_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0856967.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(1187..1384)
                     /locus_tag="WGR46_002122"
     CDS             complement(1187..1384)
                     /locus_tag="WGR46_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856968.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(1381..1647)
                     /locus_tag="WGR46_002123"
     CDS             complement(1381..1647)
                     /locus_tag="WGR46_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856969.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1659..>1932)
                     /locus_tag="WGR46_002124"
     CDS             complement(1659..>1932)
                     /locus_tag="WGR46_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856970.1"
                     /translation="SRAREQEPFVIHVSDLLDKAGIDYAVRMDKDFQTTFHLEYPITN
                     YDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVPV"
BASE COUNT          473 a          523 c          461 g          475 t
ORIGIN      
        1 cacctgttgc ctttgctgga aggtatagcc gaaggcacga ccgtctatgc cgacaaaggc
       61 tatgacagtg cggaaaaccg gcaacatctg gaagagcgtc agttgttaga cggcattatg
      121 cgcaaagccc accgcaaccg tccgctgacg gaagtgcaaa ccaaacgcaa ccggtattta
      181 tcgaagaccc gttatgtggt cgaacaaagc ttcggtacgc tgcaccgtaa attccgctat
      241 gcgcgggcag cctatttcgg gctgctcaaa gtgagtgcgc aaagccacct gaaggcgatg
      301 tgtttgaacc tgttgaaagc cgccaacagg ttaagtgcgc ccgctgccgc ctaaaaggca
      361 gcccggatgc ctgattatcg ggtatccggg gaggattaag gggatatttg ggtaaaatta
      421 ggaggtattt ggggcgaaaa cagccgaaaa cctgtgtttg ggtttcggct gtcgggaggg
      481 aaaggaattt tgcaaaggtc tcgggcttta attgcggcaa aaatagacca agcggagatt
      541 tctgattcag aaaaaagctg gctgaaaaaa gaattaggga aaatcaagga cactgcatta
      601 agtacgctta cggaaaatgc aattaatgct atccttgccg ccaccctcat tactctttta
      661 aaaaaatttg tcggcctcta accaaacttc ttctccactt ccaccccaaa cggctccaat
      721 acctccacaa aggcaggcaa atgtttgggg tgtttttctt gcacaaacgc catcaagaac
      781 tcaatcaatt ccaaagccgt cgccagtttt gacgtttccg gcatcacacg cgcattggcg
      841 gatacggttt tcgtaaacgc atcggccagg ctggccaaca gcttggcgcg gtcggacggg
      901 ggcaaatctt cggtactcga atcctgcagc atcgtcatcg tgctgttgta ctgcaccata
      961 aaaccggcca acatcgcacg gctcaagtcc tcaataccgc cgccggccaa agtgtaggcg
     1021 gcgcgcatct tatcccagtc gtcgcctttt tccttatccg cacgtttcca cgcacgcgca
     1081 gtggtctgcg ggatttcgca catcaaggcc gccgtttcca aagtttgctc gccgctcaca
     1141 tagagccgac gtaacttttc acggatttct tgcgggtgag ccataattac agtcccattt
     1201 tcgctttaag caattcccag ccgaccgtaa tcacgccgcc gcccagtgcg ccgaatgtaa
     1261 tggccgtgcg tttcgtgtct tggcggattt gcgcaatttc cgcctgcatt tctttctgat
     1321 ttttcagcgt ttgatcagtc ttgttttcaa tacgcgccaa ggcttccaaa atcgggtcgc
     1381 tcatgatttg tctgctttcc tgtccagttt ttcgtttact ttttccaact tgttttcgat
     1441 gcgtcccaac gaagccgcaa tattgtcgcg gtctgccttg gcatcttgct tggtgtgata
     1501 agagagcttg accgcgtgca gctcctcttt caggtcgtcg atacgcttgt ccgcctcttt
     1561 cagacggcct gaaatgccgt tgacccaaaa ccaaaatgcc gccgtcgcaa tcggccacag
     1621 ggttctgaaa ccaaattcaa agtccattta aaaccccttt aaaccggcac atcgccgaat
     1681 acgatacgga cggcgtaacc gtcagggcga ttgctggcaa tctccagtcc atcaccatca
     1741 ttacaaacac agtaatacgc ccccaaagtt tgccaaaccg cacgtttaaa ggtgtcatag
     1801 ttcgtaattg gatattcaag gtgaaacgtc gtctgaaaat ccttatccat gcgtaccgcg
     1861 taatcgatac cggccttatc caacaaatcg gaaacatgaa tgacaaacgg ctcttgctcg
     1921 cgtgcgcggc tt
//
LOCUS       DAWFKN010000152         1754 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFKN010000152 DAWFKN010000000
VERSION     DAWFKN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.152"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(395..1384)
                     /gene="porB"
                     /locus_tag="WGR46_002125"
     CDS             complement(395..1384)
                     /gene="porB"
                     /locus_tag="WGR46_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0856971.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          447 a          388 c          451 g          468 t
ORIGIN      
        1 atgtaggttc gtaggaatga cgtgatgcag gtttccgtat ggatggattc gtcattcccg
       61 cgcaggcggg aatctaggtc tgtcggtgcg gaaacttatc ggataaaacg gtttcttgag
      121 attttacgtt ctggattccc actttcgtgg gaatgacggg attaaagttt caaaatttat
      181 tctaaatagc tgaaactcaa cgcactagat tcccgcctgc gcgggaatga cggcatattt
      241 tgacattgaa taaaaaagac taaaaacaga aaacagccaa acagaaaaca gcgaaacagg
      301 aaaaagccaa acagaaaaaa gccaaacaga aaaaagccaa acagaaaaaa gcctgtctgg
      361 cgacaggctt tttgttgata ccaatctttg cagattagaa tttgtggcgc agaccgacac
      421 cgccggcagt cgctacgaat ttgttttcgc ctttgccttc ttgcaaccaa ccggcagaaa
      481 ccaaggcaga agtgcgtttg gagaagtcgt attccgcacc gacaaccact tggtcgtaag
      541 tattgtcgcg ttttgcatca tcaaccgagc ctttgaagcc gtgggcgtaa gaaacgcggg
      601 gcgttacgtt gccgaagcgg tatgccaagg tagcggcaac ttcggtttga gagttgtgcg
      661 aattgtcttc aaccagtttc gcgtcttgtt gctgtacggc tacggaagcg tatagggcat
      721 cattgtcgta accgctgacc aaacggtgaa tctggtattt ctcaatcttc acgtcatcca
      781 catcctgatg tcttttatag gcaccgccat attgcacgaa gaagccgccg tttttgtagt
      841 tgaagccggc gtggtaagat tcgctgttat gtctgcctgc attgtcgtta agcgcgtatt
      901 gtacgctgcc gctgaggccg gcaaattcgg gagaatcgta gcgtacggaa atgaggcgtg
      961 cctcgggttc ggcaattttg tttacaccca aatagtcgct tttgctatcc caaggattga
     1021 tgtcgccggt gtctttcagg acgctgttca aacggccgac gcgcaattta ccgaagccgc
     1081 ctttcaagcc gatgaaggat tggcggttgc cccaaccgga gtcagtaccg gcgatagatg
     1141 ctttttgctc aacctgccaa atggctttca ggccgttacc gaggtcttct tggcctttga
     1201 agccgatttt cgaacccaaa tcaacgatgc cggtaccggt ttcaacgcta taaacttgac
     1261 cgtcctggta atctagggag cgggaagttt ctacgccggc tttgatggtg ccgtacaggg
     1321 taacgtcagc cattgctgca acaggaaggg ctgccaaagt cagggcaatc agggattttt
     1381 tcattgctgt attccttttt tggttaagaa atttaagcag gccgggcttt ccaagccgct
     1441 tagctttgca tttaccgccg aggggctcgg cgattacatg tctgaaacta tagatgttcc
     1501 tgtctgaaaa aacaaacttc tgcctgtttt tgtgtggaat tcgatgtgca ttttgaagga
     1561 cggatagaga tattatgggt attttttgtt ttataacata tggttattta aattttttaa
     1621 gatttgcatt tttacaacac ttactcggga gggtattgga gggcattgca aaccgggggt
     1681 tataaagacg gcaaaaaacg cacacgcggt ttctttcgga acgggcggat gcaaaacccg
     1741 cccctgcggc agga
//
LOCUS       DAWFKN010000153         1731 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFKN010000153 DAWFKN010000000
VERSION     DAWFKN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.153"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            357..1661
                     /locus_tag="WGR46_002126"
     CDS             357..1661
                     /locus_tag="WGR46_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0856972.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          352 a          434 c          513 g          432 t
ORIGIN      
        1 caattaaaaa tttttaatat attaatcaat aaattaattt tataaaataa aaatattgtc
       61 aacaatattt tgccttatcg cccaaacctc tgtatatttt cctacagtaa attgttgaca
      121 atccatacgc ccacatatgc gccgcctaag gataaatcct cccgccggac aacgggtgca
      181 agggattgga tgcgatattt ccatattcaa acaagggatt tgtttcacgc acagggcggc
      241 acatcggcaa aatccccgcg ccgcgccggt ccggcagggc ttgcgtccct cccggacaag
      301 ccccgacccc gcctttccga aagacgggct caaccattaa ggaaacttta atcaaaatga
      361 aaaaacacat atgggcggca tctttgctgc cggcatccct atcggcagaa cctttaaact
      421 ggtggaagcc ttattccgcc gtcaattcgg gcgataccgc ctgggtgatg actgcggctg
      481 ccttggtact gttgatgacg cttcccgggc tggctttatt ctacggcggt atggtgcgga
      541 aaaaaaacct gctctcgacg atgatgcaca gcttttccat cgcgacattg gtgggcatcc
      601 tttgggtcgc cgtcggctat tctttagcgt tcacgccggg aaatgccttt atcggcggtt
      661 tggggcgcgt atttttaagc gggatgcaga tagacgctgc cgcacagatg ctgaccgtgt
      721 cgcccaatgc gccgactgtt cccgaaccgg tatttatgtt ttttcagatg acgtttgcca
      781 ttatttcgac cgccattatt accggcgcgt ttgccgaacg gatgaaatat tcggcaatga
      841 tgctgttttc gggcatatgg tttttattgg tttatgtgcc gggcgcgcat tgggtgtggg
      901 gcggcggctt tatgagcaag ggcggcgtat tggattatgc cggcggtacg gtggtgcaca
      961 tcaatgccgg tatcgcggga ctcgtcgccg ccttggtttt gggcaggcgc ataggctacg
     1021 ggcgcgaggc gatgcctccg cacaatatgg cgatgacact gatcggcgcg gcaatgttgt
     1081 ggttcggctg gttcggcttt aacgccggat cggcgcttgc ggcagacgcg gcggcgggta
     1141 tggcgatggc ggtaacgcag gtgtcggccg tattcggcgc ggcaggctgg cttgcctgcg
     1201 aaaaaatagc gggacacaaa ccttccgctt tggggctggc ttccggcgcg gtttccggtc
     1261 tggtcggcat cacccctgcc gccggcttta ccggcccgtc gggcgcggcc gccatcggta
     1321 tattgactgc cgccgcgtgc tttgtgtccg tcaccgtcgt caaacacaaa ttgcgttacg
     1381 atgattcttt ggacgctttc ggcatacacg gattcggcgg gctggtgggc ggaatattga
     1441 ccggcatctt tttcgacaac cgcattttcg gcggggatgc ggcagtttgg cagcagttgt
     1501 ggatacaggt aaaagacggg ttcgttatgg cggcatacag cgggctgatg agttgggcga
     1561 ttttgaaggt cgtggggaaa atctgcggcg gcctgcgcgt cggcaaggat gtcgaacgcg
     1621 aaggtttgga tctgaatatc cacggcgaac gcgtggaata agggcggcta tgccgtctga
     1681 agcctgaaaa tcgggtttca gacggcattt ttcacgtttg ccgccgatgg a
//
LOCUS       DAWFKN010000154         1708 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFKN010000154 DAWFKN010000000
VERSION     DAWFKN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.154"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            59..1432
                     /locus_tag="WGR46_002127"
     CDS             59..1432
                     /locus_tag="WGR46_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0856973.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1612..>1708
                     /locus_tag="WGR46_002128"
     CDS             1612..>1708
                     /locus_tag="WGR46_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ0856974.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          440 a          556 c          412 g          300 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcaggg
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtg aaacaaaaaa acatcagttt gaacaaagtc gaaatcgtcg ttctggacga
      541 agcagaccgc atgttggata tggggtttat cgacgacatc cgcaaaatca tgcagatgct
      601 gcccaaacaa cgccaaaccc tgctcttttc cgctaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      781 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccaag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1321 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaactg gactgacgca
     1501 ttgggcgcaa ccgcccgcac cggataaatt tcttccgcaa acagtttcag acggcatttg
     1561 ctgcctgtac aatatcattt ccttttccac tcaaaccatc aggatttccc aatgtccacc
     1621 caattcgatg tcgccgtaat cggcgcaggt ccggcaggct cggtcgcatc cgccctgctc
     1681 cgcaaaaaag gttatagtgg attaaatt
//
LOCUS       DAWFKN010000155         1647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFKN010000155 DAWFKN010000000
VERSION     DAWFKN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.155"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..876
                     /locus_tag="WGR46_002129"
     CDS             <1..876
                     /locus_tag="WGR46_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0856975.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRD
                     TQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIF
                     ALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQN
                     PPSFIKGTKLELKFQ"
     gene            895..1269
                     /locus_tag="WGR46_002130"
     CDS             895..1269
                     /locus_tag="WGR46_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0856976.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          516 a          340 c          369 g          422 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 attatccaag gtattatgcc tgcacatgca gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatgggg tttatgctgc aggttggaca gctccgctga tcggtacagc gggtaaatta
      241 gctatcagca cctgcatggc taatccttct ggttgtactg tcatggtcac tcaggctgcc
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagcgcct
      361 gtgggggcgt tgtcgaaagc gaaggcggct aagcaagctg ctcctaaaga aacaataaac
      421 aatttggcaa atttagccaa agcagaacag cagattttat tccgtattgc ccaacgcgat
      481 acgcaactgg atgcatggaa gacgggattt aacaatagag taaggaaagg agcaggcttg
      541 cttgatgcaa gtaatattcc gataaccatt aacggaaaaa ccatcaaacc tgtacaagcc
      601 ataagcttaa agggagcacc cgtttacagc ggcgtaagcg aacaggagat ttttgcgctt
      661 tatcggcaga tgactggcca gaatccgaat tttagagttt tgcctgacgg aagattagca
      721 aatggcatta tcagtactgg agaatgggca ggaacaaaaa ttgcattaag aaatttttca
      781 aaaacagaga attcaactca agcacgatgg acattagatt tgcagaatcc tccatcattt
      841 attaaaggta ctaaattgga gcttaaattc caataattta caaaggattt taccgtggat
      901 gagaaacaaa aaattaagat tcttgatttt caaatcgatt tatcctcaat ttttaactct
      961 tataaaaatc aaatgggtat taatattcaa gataaaaact taaaaaacaa tttctgttct
     1021 tttatggaag aactgttaaa tgacggttca atccgtttac atgattatac cgacggtatc
     1081 ggaattcctc taactggaac ttcaaaagaa caagtgcaga aattgaaaga catatggcct
     1141 actttggaag atgcccaagc aatatggcct gaagaccctt ggtattactt agaatggctt
     1201 tggtgggata ttgcgtgtcc aatagatttg gccgatttgc cgaatattga tatttatgag
     1261 caagcgtagg tatggttagc cgcctttagc ggcgtaaccg tacgcatatc agcaaacttt
     1321 ataaaataac aaggccgtct gaaatctgtt tttcaacttt ttcagacggc cttgcaactt
     1381 ggcatttcat tcgtacggtt acgccgctaa aggcggctaa ccgtacctac gagctctgat
     1441 aaaaatgatt tatggaagca agctgtagcc tgcatgaaac ctaaaatcca tgcgtaaggt
     1501 gtgtgctcca gcacgcacgc gttccatgat ttacggctca atgccgtctg aaaagctcac
     1561 aatttttcag acggcatttg ttatgcaagt aaatattcag attccctata tactgcccag
     1621 acgcgtgcgt gctgaagaca cccccta
//
LOCUS       DAWFKN010000156         1612 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFKN010000156 DAWFKN010000000
VERSION     DAWFKN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.156"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..825
                     /locus_tag="WGR46_002131"
     CDS             <1..825
                     /locus_tag="WGR46_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0856977.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRIN
                     IANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVV
                     SSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            832..1341
                     /locus_tag="WGR46_002132"
     CDS             832..1341
                     /locus_tag="WGR46_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856978.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          517 a          321 c          325 g          449 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 atcatccaaa gtattatgcc tgcacatgcg gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatggag tttatgcagc aggttggaca gctccgctga tcggtacagc tggtaaagca
      241 gctatcagca cctgtatggc taatccttct ggttgtactg tcatggttac gcaggctgct
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagctcca
      361 gtaggagcgt tgtcgaaagc gaaggcggct aagcaggcta taccaaccca gacagttaaa
      421 gaacttgatg gcttactaca agaatcaaaa aatataggtg ctgtaaatac acgaattaat
      481 atagcgaata gtactactcg atatacacca atgagacaaa cgggacaacc ggtatctgct
      541 ggctttgagc atgttcttga ggggcacttc cataggccta ttgcgaataa ccgttcagtt
      601 tttaccatct ccccaaatga attgaaggtt atacttcaaa gtaataaagt agtttcttct
      661 cccgtatcga tgactcctga tggccaatat atgcggactg tcgatgtagg aaaagttatt
      721 ggtactactt ctattaaaga aggtggacaa cccacaacta caattaaagt atttacagat
      781 aagtcaggaa atttgattac tacataccca gtaaaaggaa actaactaaa tatgagtaac
      841 tttgaaaaaa aatatatttt agaattaaat gatgctttaa gccatttaaa tcataactct
      901 acctcatttg atttattgaa agttttgatt tcatggttat caaacgatat tgtcattgat
      961 aaatttaaaa ttttaggtta tgactttagt aaatatatcg aaatgaatcc cgatgactat
     1021 ccggttgaaa aatctatatt gaatagagag gaaattattt atctcaaaaa caatatttat
     1081 cgtaaaatat catcaggaaa ttttaaattt caatactttg tacaatatat tagagatatt
     1141 ttagaatatt tatttattga acatattgaa agagtctgtc cttactgcga atggggtgaa
     1201 atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt atctctgtac tcaatgtgga
     1261 tgtgcttttt ataacgataa ttcacaattt ttattaaaaa cccctttaac cattccaatg
     1321 aaacgtgatg aatttaaata aacaagccgt agcctgcatg aaccctaaaa tccacgtgta
     1381 gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg ctcaatgccg tctgaaaagc
     1441 tcacaatttt tcagacggca tttgttatgc aagtaaatat tcagattccc tgtatgctgt
     1501 acagacgcgg gagtgttaag ccccccttgt ttgaagctcc gcggctcctg ccgagcttca
     1561 ccgaccccgt tgtgcccaag ctctctgctc ccggcggcta cattgtcgac at
//
LOCUS       DAWFKN010000157         1282 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFKN010000157 DAWFKN010000000
VERSION     DAWFKN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.157"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            54..416
                     /locus_tag="WGR46_002133"
     CDS             54..416
                     /locus_tag="WGR46_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0856979.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            447..1282
                     /locus_tag="WGR46_002134"
                     /pseudo
     CDS             447..1282
                     /locus_tag="WGR46_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          386 a          371 c          278 g          247 t
ORIGIN      
        1 tttttgttaa tccactataa tccgcacaaa tttagtcaat atcaagacca attatgaacc
       61 aactcgacca acttggcacc cgtatcaacc tgatttgcaa tgtcttcgac aaatggatcg
      121 ggcagcagga tctgaattac aacctctttg ccgtacttta taccctggca accgaaggca
      181 gccgcacgca aaagcatatc ggcgaaaagt ggagtctgcc caaacagacc gtttcaggcg
      241 tatgcaaaac ccttgccgga caagggttga ttgaatggca ggaaagcgaa caggaccggc
      301 gcgaacggtt gctgtcgctg accgaaaaag gcaaagccca tgccgcacct ttaacagaaa
      361 gcgcgcagga attcagcgac aaagagacct ttgcaaaatt ccccaaaatc ccctaaattc
      421 ccaccaagac atttaggaga ttttccatga gcaccttctt tcaacaaacc gcgcaagcca
      481 tgattgctaa acacatcgac cgtttccctc tattgaagtt ggatcaagtg attgattggc
      541 agccgatcga acaatacctg aatcgtcaaa aaacccgtta ccttcgagac caccgcggcc
      601 gtcctgccta tcccctgctg tccatgttca aagccatcct gcccggacaa tggcacagcc
      661 tctccgatcc cgaactcgaa cacagcctca ttacccgcat cgatttcaac ctgttttgcc
      721 gttttgacga actaagcatc cccgattaca gcaccttatg ccgttaccgc aactggctgg
      781 cgcaagacga caccctgtcc gaattgctgg aactgattaa ccgccaactg accgaaaaag
      841 gcctaaaaat agagaaagca tccgccgtcg ttgacgccac cattattcaa accgccggca
      901 gcaaacagcg ccaggccaga agtcgatgaa gaaggacaag tcagcggcca aaccacaccg
      961 agtaaggaca gcgatgcccg ttggataaag aaaaacggcc tctacaaact cggttacaaa
     1021 caacatacac gtaccgatgc ggaaggctat atcgagaaac tgcacattac tcccgccaat
     1081 gcccatgagt gcaaacacct gtcgccgttg ttggaagggt tacccgaagg tacgaccgtc
     1141 tatgccgaca aaggctacga cagtgcggaa aaccggcaac atctggaaga acatcagttg
     1201 caggacggca ttatgcgcaa agcctgccgc aaccgtccgc tgacggaagc gcaaaccaaa
     1261 cgcaaccggt atttatcgaa ga
//
LOCUS       DAWFKN010000158         1182 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFKN010000158 DAWFKN010000000
VERSION     DAWFKN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.158"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          269 a          382 c          288 g          243 t
ORIGIN      
        1 caagaggcat cctgaattcc ccgtcgtaac tcgagaatcc cgctgcaact cgagaaaatc
       61 cacgtggttc ccccgtcatc gcaagatgaa gccctttccc gctacagtgt ctcaggagaa
      121 gtcccacgtt aggtattgga ggtcgaaacg gtacttggca cccttgatgc gacccacaaa
      181 gtgccccgac atcccggtct ccctcaagag gaacaccgag gttttcccgc accacttcct
      241 ctgagcccct tctaccctcc tgatctggac aggagggtcg actcccctgc tttgtctgga
      301 aggggttccc gaccttccgg tcgcacctca ggatgaggcc ggtctcacga cgacattcca
      361 gacgtggcct cgtgggtggt tccacattcc gaaggacccc gatttcccgg tcccctcttg
      421 ataagaaccc gatgcccgga cacctcttcg aactccaccc tgtgaatgaa gtcaacacga
      481 aggggcagtg actcgcccgt gcatcgtcgg gaaaagaccc ccaggttcca aataccgctc
      541 gacaagtggc ctgtctcccc gggaacacct cgagaggcaa gcggagttcc atgcctcaac
      601 ccaagacgag gcctgactct cctgtcccaa gtctgcaggg accttgcgat cggagtctga
      661 agtcagagga accctgaggt tcctgcctca actggagatg aggccctctt ccaatgcacc
      721 aaacccagtg gagtgccgag aggcccctcc cacctccagt ttccctgact tctcagagcc
      781 accatgagaa gccccctgag gtcacctgca caagtcgagg gaccccaggg tgtcctgcct
      841 caacccgaga aagacctcga gagaccttct tcaacacgtc tcgaggccag attcccctac
      901 tgccgtgagc cggcatattg catattgagt gcatattgca tattgcatat tgagtgcagc
      961 actttccaca gcatcatctt tcaggatctg gaatagctcc actggaattc tatcactgct
     1021 gggagccagt gaggaactcc gcccatgaca aaggtcatga ggaaggaggc tcggcatacg
     1081 caaaggcggg atcgagcttc aggagtcccc ctggaaattc tcgagcatat acccccaaaa
     1141 ccagagtctg cctactttct gcttgtgctt tcacctaaac ct
//
LOCUS       DAWFKN010000159         1150 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFKN010000159 DAWFKN010000000
VERSION     DAWFKN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.159"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          238 a          401 c          292 g          219 t
ORIGIN      
        1 aggcaggcgg agttccatgc ctcaacccaa gacgaggcct gactctcctg tccccagtct
       61 gcagggaccc tgcgatcgga gtctgaaatc agaggtaccc tgcggttcct gcctcaactg
      121 gagatgaggc cctcttccaa tgcaccaagc ccagtggagt cccgagaggc ccctcccacc
      181 tccagtttcc ctggcttctc agagccacca tgagaagccc cctgaggtca cctgcacaag
      241 tcgagggaac ccagggtttc ctgcctcaac ccgggaaagc cctcgagaga ccttcttcaa
      301 cacgtctcga ggccagattc ccctaccatg gctcgggagc aatgacgcgc tccccctcgc
      361 cactcgcatg gagacccgac ttccctggcg ccccacgaga ggctcactga cctcgccgtc
      421 gtacctcgtg agaaaccgca cactggggcc gccgctcgag aacaaccccg agattccccc
      481 gtcatcgaga gatgagggcc ttcgtctcct gcatggccta gcgaccaatc tcgcgacctc
      541 tctccaaacg cctcaggagg cttgactccc ttgagtccac ccagtgagct ccaagagata
      601 cccgtcgcga ttcgagagca gagcggggtt ctttgcttcc actcgagatg aatgcctgtc
      661 tccccgggtg cgtctggaat gcaaccccga gatccctgtc gcccctggag aggaacattg
      721 gcttctggac acaagcctag atgaggtcta ttggccctgc agtcactcga gagcaatccc
      781 cagctttcct tcgcaactcg aatggaagat tggacttgcc tgggccaaca caagaggcag
      841 cctgaattcc ccgtcgtaac tcgagaatcc cgccgtaact cgagaaaaac cacgtggctc
      901 ccccgtcatc gcaagatgaa gccctttccc gctacagcgc ctcaggagaa gtcccacgtt
      961 aggaattgga ggtcgaaagg gcacttggca cccttgatgc gacccacaaa gttccccgaa
     1021 atcccggtct ccctcgagag gaacaccgag gttttccggc accccctcct ctgagccctt
     1081 tctcccctcc tgatctggac aggagggtcg actcccctgc tttgtctgga aggggttccc
     1141 gaccttccgg
//
LOCUS       DAWFKN010000160         1144 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFKN010000160 DAWFKN010000000
VERSION     DAWFKN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.160"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..422)
                     /locus_tag="WGR46_002135"
     CDS             complement(<1..422)
                     /locus_tag="WGR46_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0856980.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKL"
     gene            complement(542..748)
                     /locus_tag="WGR46_002136"
     CDS             complement(542..748)
                     /locus_tag="WGR46_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856981.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
     gene            1038..1144
                     /locus_tag="WGR46_002137"
                     /pseudo
     CDS             1038..1144
                     /locus_tag="WGR46_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          285 a          310 c          224 g          325 t
ORIGIN      
        1 tccaacttgt cgcagctgct cagactatcg gtataaacaa tgctgtccgg catgattttc
       61 tttttgatga cagggagtaa cgtttcagac ttggcattgt ttacgacaac ggtatagacc
      121 cgtccgttgc gtttcagaat gccgaagaca accacttttc ctgccgcacc gcgaccacgt
      181 ctgcctttac gccgtccgcc gaaatcgctt tcgtccggct cgacagagcc ctcaaaaacc
      241 tcatcggcag ccaaggctaa atgatggttg ataaccgtgc ggattttacg gtagaacagt
      301 gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt aacttcgagt
      361 acaaaaaaac ggagcagttc tttctgtact tttttcttta atttgcagtg cgttatcttc
      421 atatttcgag ggtaacatat ctgctaatct agtacagccc ctagtttaaa gctttgtttc
      481 ttaagtccgc agagtatgcc atggttagac cttcaaagtt gagtattgta ctattttgtt
      541 tttaattaac tataaaacgc ttgtgtgaat aggaagactg cagaatattg ctgccttttc
      601 ctgtttgctt taaataaaca gaaaatgccg tctgaaaccg attttcagac ggcatttgtc
      661 ccttaaaaca cgttttttca agcgcgacca taccaaacac ccacaggttg ccttccaccc
      721 ttcgtacaac ctttgctcca tcaaacatcc acttcctaaa tactgatgtc tgcttaggtt
      781 gaatcggcat tcgtacgatt acggcacacc gcctaatctg tacctgcgtc tgttgcgccc
      841 aataccaaag actacctgaa aatctcccaa acttttcagg tagccttatc acgttttcga
      901 ctgttaccac acgcccaaag accgcctgaa accctttttc agacgttccg ctccgatacc
      961 ccccaacaaa acgccgcccg aacgcatgtt cagacggcat tttctttgcg tcgattcatt
     1021 ccccgatata gcgcaggtct ttttccagcc atttcccttc caaacaaaaa agcaccgacg
     1081 gcggccgatg ccccttcctt tacaggttcc cctatttttt agctgcgggc agagccggtt
     1141 ttgc
//
LOCUS       DAWFKN010000161         1074 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFKN010000161 DAWFKN010000000
VERSION     DAWFKN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.161"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            32..>1074
                     /gene="tuf"
                     /locus_tag="WGR46_002138"
     CDS             32..>1074
                     /gene="tuf"
                     /locus_tag="WGR46_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0856982.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIA"
BASE COUNT          292 a          298 c          267 g          217 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gcgagaacgt aaccatcacc gtagaactga ttgc
//
LOCUS       DAWFKN010000162         1053 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFKN010000162 DAWFKN010000000
VERSION     DAWFKN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.162"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(124..552)
                     /locus_tag="WGR46_002139"
     CDS             complement(124..552)
                     /locus_tag="WGR46_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0856983.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WGR46_002140"
     CDS             complement(533..967)
                     /locus_tag="WGR46_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0856984.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          268 a          315 c          188 g          282 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcatcc
       61 cctgcaaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 cttcttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttgccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacccc atcatcccca cccgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc cagcattgcc gctgatgatg tccgacgcag atgcggtttg ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cagaaacatc catatccgca cacgaccgct tatccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc atccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcattat ctctccaata caacatatag tataaattag cgcattttat acaataaaat
     1021 acaatatata gtattaagcc gatgtttttt tgc
//
LOCUS       DAWFKN010000163         1046 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFKN010000163 DAWFKN010000000
VERSION     DAWFKN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.163"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            158..>1046
                     /locus_tag="WGR46_002141"
     CDS             158..>1046
                     /locus_tag="WGR46_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0856985.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEHYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVVDATIIQTAGSEQRQAIE
                     VDEEGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDGEGYIEKLHITPANAHECK
                     HLSPLLEGLPEGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRPLSEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKF"
BASE COUNT          296 a          318 c          230 g          202 t
ORIGIN      
        1 tttatccgaa atcacggcaa cttttccacc gtcattccca cgaaagtggg aatccaggtc
       61 tgtcggcacg gaaacttatc gagaaaaacg gtttgagacc tttgcaaaac tccccaaaat
      121 cccctaaatt cccaccaaga catttaggag atttctcatg agcaccttct tccaacaaac
      181 cgcgcaagcc atgattgcca aacacatcga ccgtttcccg ctattgaagt tggatcaggt
      241 aattgattgg cagccgatcg aacactacct gaaccgtcaa agaacccgtt accttcgaga
      301 ccaccgcggc cgtcccgcct acccgctgct gtccatgttc aaagccgtcc tgctcggaca
      361 atggcacagc ctctccgatc ccgaactcga acacagcctc attacccgca tcgacttcaa
      421 cctgttttgc cgtttcgacg aactgagcat ccccgattac agcaccttat gccgctaccg
      481 caactggctg gcgcaagacg ataccctgtc cgaattgctc aaactgatca accgccaact
      541 gaccgaaaaa ggtttaaaaa tagagaaagc atccgccgcc gttgttgacg ccaccattat
      601 tcagaccgcc ggcagcgaac agcgtcaggc catagaagtt gacgaagaag gacaagtcag
      661 cggccaaacc acaccgagta aggacagcga tgcccgttgg atcaagaaaa acggcctcta
      721 caaactcggt tacaaacaac atacccgtac cgatggggaa ggctatatcg agaaactgca
      781 cattaccccc gccaatgccc atgagtgcaa acacctgtcg ccgttgttgg aagggttacc
      841 cgaaggtacg accgtctatg ccgacaaagg ctacgacagt gcggaaaacc ggcaacatct
      901 ggaagaacat cagttgctgg acggcattat gcgcaaagcc tgccgcaacc gtccgctgtc
      961 ggaagcgcaa accaaacgca accgatattt gtcgaagacc cgttatgtgg tcgaacaaag
     1021 cttcggtacg ctgcaccgta agttcc
//
LOCUS       DAWFKN010000164         1041 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFKN010000164 DAWFKN010000000
VERSION     DAWFKN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.164"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..353
                     /locus_tag="WGR46_002142"
     CDS             <1..353
                     /locus_tag="WGR46_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856986.1"
                     /translation="LAVSHHQGYKSAFAKQQAVIDKMEKDKAQALLLSAQNYARELEQ
                     ARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCIDGFGH
                     HGLQLYKRALGYGN"
     gene            250..555
                     /locus_tag="WGR46_002143"
     CDS             250..555
                     /locus_tag="WGR46_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856987.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(600..770)
                     /locus_tag="WGR46_002144"
     CDS             complement(600..770)
                     /locus_tag="WGR46_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0856988.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            783..1001
                     /locus_tag="WGR46_002145"
     CDS             783..1001
                     /locus_tag="WGR46_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0856989.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
BASE COUNT          274 a          241 c          294 g          232 t
ORIGIN      
        1 tcttggcggt gtcgcaccat caaggctaca agtcggcttt tgcgaagcag caggcggtca
       61 ttgacaagat ggaaaaagac aaagcgcaag ccctgctgtt gtcggctcaa aactacgccc
      121 gcgagctgga acaggcgcgt gcggaagcta aaaaatatga agtcaaggcg cacgccgtcg
      181 gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa aataaaaagg
      241 aaatcgaaaa tgtccttact caagaccgta aaaatgcagg cggcggttgt attgacggct
      301 ttggccatca cggcttgcag ctctacaagc gcgccctcgg ctacggaaat taaggttgtc
      361 gaaaaggcgg tcatgccgac accgcctgcc gcgttgatgg tcgcgccggt gcgcccgaat
      421 ccgccgaaag acggcaagac ggccacgctg ttggaacacg ccgccgagtt tggcggctat
      481 gttgccgaac ttgaaaacca aaatcaggct tggcgcgact gggcgggcaa tcactcccgc
      541 aaagtcggaa actgacaaaa aagcccgcgt agggcgcggg ctgagggtga aagcggattt
      601 tatacctctt ttacaggggt agcggcggta gtgcttttca gcaaatcgac tgcgtgctgg
      661 cagttttgct tgctggtgta gccttcgccc tgagcgatga tttcatggtt ggctgctttc
      721 aaacgccaac ggtattcgcc ttttgcgtct ttatagattt caaaatacat aaggtttctc
      781 ctatgaatga gtacacgttt tcttaccgct ttaacggcaa gtcctggtca ttgagcattt
      841 gggcggacaa ccctgaagaa gccagggcga aatttcgggc tgcacgagaa aatgcgcact
      901 atgacggcga agttgtagca aaggtttata catttgtaaa tatttcgtgg gttaagaaat
      961 tgtacaagcg gacaaaatat ttaatgggta tcaaagaatg acctaccgtg aattagttga
     1021 acgtcagttg gctgtgcgcc a
//
LOCUS       DAWFKN010000165         1032 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFKN010000165 DAWFKN010000000
VERSION     DAWFKN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.165"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            238..909
                     /locus_tag="WGR46_002146"
     CDS             238..909
                     /locus_tag="WGR46_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0856990.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          295 a          243 c          224 g          270 t
ORIGIN      
        1 attttgcaaa ggtctcaaac ggatgaaatc aaccgatatt caaacacagt catttttagc
       61 gcattttcag cgtatcgtta atgcggaaaa tttcgtgaac aggttttttg cacaggtctc
      121 cgcctcgttt cgcgggatgg gaaaccgtat tagaaaacgg acgcacgcaa cataaacccc
      181 gaaagtgatg ataagatgat gatttaacgt actgctttaa ttatttaagg aattatcgtg
      241 tttcccgaca aatacaagtt gagtttggaa gaaaatattt ttctggcaaa gaaagtattg
      301 gttgcccaaa ttcacaacct cagccgtttt gagaattgtc agacgacctt gttgcagacc
      361 gaacaaatta tccatggcaa aaatgtagcc tccgcgtcac tggaagacat ccaaaccatc
      421 ttgaacctga aacgtgccta tcaatatgtg atttcgcata tttcaaacgg cgaaccggtc
      481 gatatttcac tccttaaaaa aatcaacaac attgttgcca aggacgattc tttggcaccc
      541 ggtgatttcc gtaccggttc ggtcggcgta acgctattgg acggttcccg tcatgcccca
      601 aatccggtga aggaaattga agtggctcgc gtgttacaaa atatcggact gcaaagcggt
      661 tcgacgacgg aggcagccgt ccgttttatg ctttattgta tgcggcagca ggttttttgg
      721 gacggcaaca aacgaacggc aaccttattt gccaacggtc tgatgatggc ggggggctgc
      781 ggcatcttgg aaatctccga aatgcagatg ccgcaattca atgaaaaact gtccgcattc
      841 tatcgctccg gcgacgatac cgatatttcc aagtttgtgt atcaaaattg tatatcgggc
      901 atagactgag acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag
      961 gggatttctc atgagcacct tcttccggca aaccgcccaa gccatgattg ccaaacacat
     1021 cgaccgcttc cc
//
LOCUS       DAWFKN010000166          933 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFKN010000166 DAWFKN010000000
VERSION     DAWFKN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.166"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>933
                     /locus_tag="WGR46_002147"
     CDS             <1..>933
                     /locus_tag="WGR46_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0856991.1"
                     /translation="KQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIV
                     VIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQA
                     AVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSS
                     TGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASK
                     IKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSD
                     AEKHKVISYSKIIAGSVAALNGGDVNTAANAAEV"
BASE COUNT          270 a          226 c          255 g          182 t
ORIGIN      
        1 ccaaacagcc cgaatacgcc tacctgaaac agcttcagac ggccaagaac gtcgattgga
       61 aacaggtgca gctggcctac gacaaatggg actataaaca ggaaggcttg accggagccg
      121 gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga gcggctgcag
      181 cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg acagcggcag
      241 caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc tccttgtata
      301 gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca ttaaaagatc
      361 tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg ggtgcattaa
      421 accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa ctgttcagca
      481 gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac ctcagcaatg
      541 caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa gacaacttag
      601 gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc agcaaaatca
      661 aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg gcgggttgtg
      721 ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca gttggggaaa
      781 tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat gcagaaaagc
      841 ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc aacggcggcg
      901 atgtgaatac tgcggcgaat gcggctgagg tag
//
LOCUS       DAWFKN010000167          858 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFKN010000167 DAWFKN010000000
VERSION     DAWFKN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.167"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            1..687
                     /locus_tag="WGR46_002148"
                     /pseudo
     CDS             1..687
                     /locus_tag="WGR46_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          244 a          226 c          229 g          159 t
ORIGIN      
        1 taccgcaact ggctggcgca agacgacacc ctgtccgaat tgctggaact gattaaccgc
       61 caactgaccg aaaaaggctt aaaaatagag aaagcatccg ccgccgtcgt tgacgccacc
      121 attattcaga ccgccggcag caaacagcgt caggccatag aagttgacga agaaggacaa
      181 gtcagcggcc aaaccacacc gagtaaggac agcgatgccc gttggataaa gaaaaacggc
      241 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
      301 ctgcacatta cccccgccaa tgcccatgag tgcaaacacc tgtcgccttt gctggaaggg
      361 atagccgaag gtacgaccgt ctatgccgac aaaggctatg acagtgcgga aaaccggcaa
      421 catctgaaag aacatcagtt accggacggc attatgcgca aagcctgccg caaccgtccg
      481 ctgacggaaa cgcaaaccaa acgcaaccga tatttgtcga agacccgtta tgtggtcgaa
      541 cagagcttcg gtacgctgca ccgtaaattc cgctatgcgc gggcagccta tttcgggctg
      601 ctcaaagtga gtgcgcaaag ccacctgaaa gcgatgtgtt tgaacctgtt gaaagccgcc
      661 aacaggctaa gtgcgcccgc tgccgcccaa aaggcgaccg gatgcctgat tatcgggtat
      721 ccggggagga ttaagggggt atttgggtaa aattaggagg tatttggggc gaaaacagcc
      781 gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa aggtctcttt
      841 tcgtcattcc cgccactt
//
LOCUS       DAWFKN010000168          852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFKN010000168 DAWFKN010000000
VERSION     DAWFKN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.168"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGR46_002149"
     CDS             <1..318
                     /locus_tag="WGR46_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0856992.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
     gene            355..621
                     /locus_tag="WGR46_002150"
                     /pseudo
     CDS             355..621
                     /locus_tag="WGR46_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          220 a          181 c          233 g          218 t
ORIGIN      
        1 catcttcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaat atactatccg cgatgaggcc gattttgcct ggcattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttccg attgggggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatttatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattggat acgattgaag taatgtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggc tgcaggacgg cattatgcgc aaagcctgcc gcaaccgtcc gctggcggaa
      421 gtgcaaacca aacgtaaccg atatttatcg aagacccgtt atgtggtcga gcaaagcttc
      481 ggtacgctgc accgtaagtt ccgctatgct cgggcagcct atttcgggct gctcaaagtg
      541 agtgcgcaaa gccatctgaa agcgatgtgt ttgaaccttt tgaaagccgc caacaggcta
      601 agtgcgcccg ctgccgccta aaaggcagcc cggatgcctg attatcgggt atccggggag
      661 gattaagggg gtgtttgggt agaattagga ggtatttggg gcgaaaatag acgaaaacct
      721 gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggcctca agatatagtg
      781 gattaaattt aaatcagtac ggcaaggcga ggcaacgctg tactggttta aatttaatct
      841 actatatttc cg
//
LOCUS       DAWFKN010000169          778 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFKN010000169 DAWFKN010000000
VERSION     DAWFKN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.169"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..733
                     /locus_tag="WGR46_002151"
     CDS             <1..733
                     /locus_tag="WGR46_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0856993.1"
                     /translation="QFAQSQPLTKTDAKDAKMPAFFAQMTAQKEDSQTMPYQPPTEAE
                     EVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHTH
                     THFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESGETKFK
                     SRCFGGRSAVRKALYMATVAATRFKPLIRDFYQRLPSEGKPYKVAVTACMRKLLTISN
                     ARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          212 a          209 c          209 g          148 t
ORIGIN      
        1 tcagtttgcc caatcgcagc cgctgaccaa aaccgacgcc aaagatgcca aaatgcccgc
       61 cttcttcgca cagatgacgg cacagaaaga agattcgcaa accatgccct accaaccgcc
      121 caccgaagcg gaagaagtgt tggaagcctt ggttaaccgc cgcaaccaat cggcggatat
      181 gcggactgcc gagaaaaacc gtctgcatca ggttcacgaa acgcaagtcg gaagcgtcaa
      241 acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa tcgacaacca
      301 cacccacacg cattttgacg gcaaagccca agtggcagag caaatcaaag gcatcggttc
      361 gataacgacg gctacgctga tggcgatgct gcccgaattg gggcggctgt cgcacaaacg
      421 gatagcgagt ttggccggca ttgccccgca cccgagggag agcggggaaa ccaaattcaa
      481 aagccgctgc ttcggcggaa ggtctgcggt acgtaaggcg ctgtatatgg ctaccgtggc
      541 agcgacacgt tttaaaccgc ttattcggga tttctaccaa cgcctgccgt ccgagggtaa
      601 gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctgacgatat cgaatgcccg
      661 gatgcgtgat tattttgccg aaaacgatac cgccgaaaac gataccgccg aaaacggtat
      721 ccggacggct tgatttgagt tttggtattt ttgcccgacg gggtgaaaaa tacagttg
//
LOCUS       DAWFKN010000170          748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFKN010000170 DAWFKN010000000
VERSION     DAWFKN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.170"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..224
                     /locus_tag="WGR46_002152"
     CDS             <1..224
                     /locus_tag="WGR46_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0856994.1"
                     /translation="TAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDT
                     TAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            228..>748
                     /gene="mafB"
                     /locus_tag="WGR46_002153"
     CDS             228..>748
                     /gene="mafB"
                     /locus_tag="WGR46_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0856995.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTAR"
BASE COUNT          208 a          273 c          169 g           98 t
ORIGIN      
        1 aaaccgccgc ctacgaatcc caataccaag aacaatacgc cctctggatg ggaccttaca
       61 gcgtcggcaa aaccgtcaaa gcctcagacc gcctgatggt cgatttctcc gacatcaccc
      121 cctacggcga cacaaccgcc caaaaccgtc ccgacttcaa acaaaacaac ggtaaaaacc
      181 ccgatgtcgg caacgaagtc atccgccgcc gcaaaggagg ataaaccgtg aaaccgctgc
      241 gcagactgac aaacctcctt gccgcctgcg ccgtagcggc ggccgcactc atacagcccg
      301 ccctcgcagc ggacttggcg caagacccgt tcattaccga taacacccaa cggcagcact
      361 acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt tccgaccgca
      421 ccggcaaaat caacgtcatc caagactata cccaccagat gggcaacctg ctcatccaac
      481 aggcggcaat ccaaggcaat cttggttaca ccgtccgctt ttccggacac ggacacgaag
      541 aacacgcccc cttcgacaac cacgccgccg acagcgcaag caaagaacaa ggcaacgttg
      601 acgacggctt taccgtgtac cggctcaact gggaaggaca cgaacatcat cccgccgatg
      661 cctacgacgg cccgaagggc ggcaattacc ccaaacctac gggcgcacga gacgaataca
      721 cctatcacgt caacggcaca gcccgcag
//
LOCUS       DAWFKN010000171          748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFKN010000171 DAWFKN010000000
VERSION     DAWFKN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.171"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..224
                     /locus_tag="WGR46_002154"
     CDS             <1..224
                     /locus_tag="WGR46_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0856996.1"
                     /translation="TAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDT
                     TAQNRPDFKQNNGKTPDVGNEVIRRRKGG"
     gene            228..>748
                     /gene="mafB"
                     /locus_tag="WGR46_002155"
     CDS             228..>748
                     /gene="mafB"
                     /locus_tag="WGR46_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0856997.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGSVNEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTAR"
BASE COUNT          210 a          278 c          167 g           93 t
ORIGIN      
        1 aaaccgccgc ctacgaatcc caataccaag aacaatacgc cctctggatg ggaccttaca
       61 gcgtcggcaa aaccgtcaaa gcctcagacc gcctgatggt cgatttctcc gacatcaccc
      121 cctacggcga cacaaccgcc caaaaccgtc ccgacttcaa acaaaacaac ggtaaaaccc
      181 ccgatgtcgg caacgaagtc atccgccgcc gcaaaggagg ataaaccgtg aaaccgctgc
      241 gcagactgac aaacctcctt gccgcctgcg ccgtagcggc cgccgcactc atacagcccg
      301 ccctcgcggc ggacttggcg caagatccgt tcattaccga taacgcccaa cggcagcact
      361 acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt tccgaccgca
      421 ccggcaaaat caacgtcatc caagactata cccaccagat gggcaacctg ctcatccaac
      481 aggcaaacat caacggcaca atcggctacc acacccgctt ttccggacac ggacacgaag
      541 aacacgcccc cttcgacaac cacgccgccg acagcgcaag cgaagaaaaa ggcagcgtca
      601 acgaaggctt taccgtatac cgcctcaact gggaaggaca cgagcaccat cccgccgatg
      661 cctacgacgg cccgaagggc ggcaattacc ccaaacctac gggcgcacgc gacgaataca
      721 cctatcacgt caacggcaca gcacgcag
//
LOCUS       DAWFKN010000172          740 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFKN010000172 DAWFKN010000000
VERSION     DAWFKN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.172"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            71..>740
                     /locus_tag="WGR46_002156"
     CDS             71..>740
                     /locus_tag="WGR46_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0856998.1"
                     /translation="MQADLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGDWRIAADYASYRKWNNNKYSVNTKELENKNNNKRDLKTENQENGSFHAASS
                     LGLSAIYDFKLNDKFKPYIGARVAYGHVRHSIDSTKKTTEVLTSSHAPGTAPTIYNQG
                     STQNAYHESHSIRRLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLG
                     MRYRF"
BASE COUNT          213 a          217 c          159 g          151 t
ORIGIN      
        1 cttctcttct cttctcttct cttccgcagc gcaggcggca agtgaagacg gcagccgcag
       61 cccgtattat gtgcaggcgg atttagccta cgccgccgaa cgtattaccc acgattatcc
      121 gaaagcaacc ggtgcaaaca acacaagcac agtaagcgat tatttcagaa acatccgtgc
      181 gcattccatc cacccccggg tgtcggtcgg ctacgatttc ggcgactgga ggatagcggc
      241 agattatgcc agttacagaa aatggaacaa caataaatat tccgtaaaca caaaagagtt
      301 ggaaaacaag aataacaata agagagacct gaagacggaa aatcaggaaa acggcagctt
      361 ccacgccgct tcttctctcg gcttatccgc catttacgat ttcaaactca acgataaatt
      421 caaaccctat atcggtgcgc gcgtcgccta cggacacgtc agacacagca tcgattcgac
      481 caaaaaaaca acagaggttc ttacctcctc ccatgctcct ggcacagcac ctacgattta
      541 taatcaggga agtacgcaaa acgcctatca cgaaagccac agcatccgcc gcttaggtct
      601 tggtgtcatc gccggtgtcg gtttcgacat cacacccaag ctgaccttgg acaccggata
      661 ccgctaccac aactggggac gcttggaaaa cacccgcttc aaaacccacg aagtctcatt
      721 gggcatgcgc taccgcttct
//
LOCUS       DAWFKN010000173          727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFKN010000173 DAWFKN010000000
VERSION     DAWFKN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.173"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          192 a          161 c          181 g          193 t
ORIGIN      
        1 tgattatagc ctgctgactg attcctgcct actcagctct atgttcattg cctccagagg
       61 cacatgatca gtgtgtgaca ccaaacatga tcagcacatg atcagtcaca tgatcaatgc
      121 atgataagcc aggtgaacag tgcatgatca attatgtgat cagtgcatga tcagtcatgt
      181 gaccagtaca tggtagctgc gtgatcattg catgatcagc cacgtgagca gtgcatgata
      241 gccacgtgat cagtgcatga tcagtcacgt gatctgtgca tgatcagtca cgtgatctgt
      301 gcatgatcag tcacgtgatc agtgcatgat caattacctg atcagtgcat gatcagtcac
      361 gtgaccagtg catggtagct acgtgatcat tgcatgatca gccacgtgat cagtgcatga
      421 tagccacgtg atcagtgcat gatagccacg tgatcagtgc atgatcagtc acgtgatacg
      481 tgtgtgatca gccacatgat cagtgcatga tatccacgtg atcagtgcag gatagccacg
      541 tgatcacttc atgattattc acgtgatctg tacatgatca gtcacgtgat acgtgtgtga
      601 tcagccaccg gatcagtgcg tgatgagcca tgtgatcagt gcaggatcaa ttatgtgatc
      661 agtgcatgat cagtcacgtg atcagtgcat gatcagtcac gtgatcagtg tatgatcagc
      721 cacgtga
//
LOCUS       DAWFKN010000174          717 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFKN010000174 DAWFKN010000000
VERSION     DAWFKN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.174"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          220 a          200 c          143 g          154 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttctg ttgtccaaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accccccccc cgaatttgcg taaaataaag tgtaatccct ttgattataa
      181 gggaatgttg acaattagat cgattctcct aggctgactt tcgcaataaa tggggttgtc
      241 aataattgtg tatggtcgtt tgccgtccgg cggccgtcat tcccgcgcag gcgggaatcc
      301 agcaacacaa cgcagcagga ctttatcgga aaaaaccgaa accccaccga ccgtcattcc
      361 cgcaaaagcg ggaatccagc aaccgaaaaa ccacaggaat ctatcagcaa aaacagaaac
      421 cccaccgccg tcattcccgc gaaagcggga atccagaccc gtcggcacgg aaacttaccg
      481 gataaaacag tttccttaga ttccacgtcc tagattcccg ccttcgcggg aatgacgaga
      541 ttttagatta tgggaattta tcaggaatga ttcaatccat agaaaaacca caggaatcta
      601 tcagcaaaaa cagaaacccc caccgcgtca ttcccgcaaa agcgggaatc cagaaacaca
      661 acgcggcagg actttatcgg aaaaaacaga aacccccacc accgtcattc ccgcgca
//
LOCUS       DAWFKN010000175          715 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFKN010000175 DAWFKN010000000
VERSION     DAWFKN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.175"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          203 a          174 c          170 g          168 t
ORIGIN      
        1 gcactgatca cgtggatgat catacactaa tcatgtgaca gatcatgcac tgatcacgtg
       61 gctgatcatg cactgatacc gtgactgagc atggactgat cacatgacta tcatgtagtg
      121 atcacgtgac ttaatggcgc tgatcatgtg actgatcatg cgctgatcat gtttgctatc
      181 atacactgat catgtgcctc tggaggcaat aaacaaagag ctgagtaggc acgaatcaaa
      241 cagtcagcag gcaataatca tggaactcag ctgtgaggaa tcatgctgct cagctggcaa
      301 taatcaagca gctgagcagg caggaattac acagcacagc tggcaattgt caagcagatg
      361 acaggcagga atcgtgcagc tcagctggca attgtcaagc agatgagcag acactaatca
      421 cgcagctcag caggcccgga tcacgtgact gagcatgcac tgttcacgtg gctgatcata
      481 cactgatcac gtgattgatc atgcactgat cacatggata tcatgcactg atcacgtggc
      541 tgatcataca ctgttcacgt gtctgaccat gcactgatca cgtggctgat catacactgc
      601 tcacgtaact gatcatgcac tgatcacgtg gctatcatgc actgaatacg tggctgaaca
      661 tgcactgttc acgtggctga tcacacacct gtcacgtgac ataacatgca ctgat
//
LOCUS       DAWFKN010000176          686 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFKN010000176 DAWFKN010000000
VERSION     DAWFKN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.176"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          117 a          239 c          220 g          110 t
ORIGIN      
        1 aaacctggga ccaaagtaac tgcccggagc tcactgatca tgggctggtt cccctgcgct
       61 gcggctgtgc tagggagccc aaacgcccag gggaagcccg cacagcacat ccacctgcct
      121 ctaggcggga ggacagctcc agggaaggcc cggtctggca ggcgctttct gggcacaaac
      181 ggagcaacac tctggctgtg gagctgcctc cccaagccag gggcaccggg accctgattc
      241 cctccgtgcg gcgcttagtc tgggcgggga agcaccagct tgggaatgca gcctggggcg
      301 cttcctttcc ctcggaaaga gctctccccg cgggaggctt tggcagtgtg cacaccccca
      361 gcccctaagc cctctgcaag gacgcctggc tcctaggtcc gaagggaagg cgagagctga
      421 gctgaggcag cagtcaggca ccactcacgg ctcccacact cccgcagcct gctccttccc
      481 gggcgcgaac gctagaagct gggcagggct tggggaggct cgcccagcac agagcagcct
      541 ctcggccggg gctctgtgct atcgcgcctg ggcgcagagc tggagctccg ccggtgtgga
      601 agctcactcg gtccttccca gtacaggctt gcaagcaccc gccggcagcc agcctcttct
      661 ctcccgcgtt ccgggcattg ggctcc
//
LOCUS       DAWFKN010000177          682 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFKN010000177 DAWFKN010000000
VERSION     DAWFKN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.177"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>682
                     /locus_tag="WGR46_002157"
     CDS             <1..>682
                     /locus_tag="WGR46_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0856999.1"
                     /translation="IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKL
                     DSHKKRKFLGITYDKVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGG
                     AVIRAGVGDQARADAKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFT
                     GPVAPVLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDK
                     WDYKQEGLT"
BASE COUNT          210 a          190 c          159 g          123 t
ORIGIN      
        1 atcgacatca aatctggtgg cagcatcgac gcctacgcca ccgtattcga tgcccccaaa
       61 ggcagcatta acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac
      121 tacgacaaac tagacagtca taagaaacgc aaatttctcg gcattactta tgataaggta
      181 catgatacct ctacccaaca gatgcaaact gccttaccaa gccgtgtagt ggctgaatca
      241 gctaatctgc aatcaggttg ggataccaag ttacaaggta cgcagtttga aaccacgctg
      301 ggcggcgcgg tcatccgtgc aggcgtaggc gaccaggcac gggccgatgc caagattatc
      361 ctcgaaggga tcaaaagcag catccacaca gaaaccgtaa gcagcagcaa atccgccctc
      421 tggcaaaaac aggcaggacg cggcagcaac atcgaaaccc tgcaactgcc aagtttcacc
      481 ggccccgttg cgcccgtact ctctgctccc ggcggctaca tcgtcgacat tccgaaaggc
      541 aatctgaaaa ccgaaatcga aaaactcgcc aagcagcccg agtatgctta tttgaaacaa
      601 cttcaagtag cgaaaaacat caactggaat caggtgcagc ttgcttacga taaatgggac
      661 tacaaacagg agggcttaac cg
//
LOCUS       DAWFKN010000178          679 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFKN010000178 DAWFKN010000000
VERSION     DAWFKN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.178"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(1..429)
                     /locus_tag="WGR46_002158"
                     /pseudo
     CDS             complement(1..429)
                     /locus_tag="WGR46_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            492..>679
                     /locus_tag="WGR46_002159"
     CDS             492..>679
                     /locus_tag="WGR46_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0857000.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKA"
BASE COUNT          136 a          186 c          164 g          193 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttaaattta atccactata acgggtcttc gacaaatacc
       61 ggttgcgttt ggtttgcgtt tccgacagcg gacggttgcg gcaggctttg cgcataatgc
      121 cgtccaacaa ctgatgttct tccagatgtt gccggttttc cgcactgtcg tagcctttgt
      181 cggcatagac ggtcgtacct ttgggcagtc cttccaacaa cggcggcagg tgtttgcact
      241 catgggcatt ggcgggggta atgtgcagtt tctcgatata gccttccgca tcggtacggg
      301 tatgttgttt gtaaccgagt ttgtagagat ccaacgggca tctttgtctt tactcggtgt
      361 ggtttggccg ctgatttgtc cttcttcgtc aacttctatg gcctggcgct gtttgctgcc
      421 ggcggtctga gacctttgca aaattcccca aaatccccta aattcccacc aagccattta
      481 ggggattttc catgagcacc ttcttccggc aaaccgcaca agccatgatt gccaaacaca
      541 tcgaccgttt cccgctattg aagttggacc aggtgattga ttggcagccg atcgaacaat
      601 acctgaaccg tcaaaaaaac cgttacctta gagaccaccg cggccgtccc gcctaccctc
      661 tgctgtccat gttcaaagc
//
LOCUS       DAWFKN010000179          638 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFKN010000179 DAWFKN010000000
VERSION     DAWFKN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.179"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>638)
                     /locus_tag="WGR46_002160"
     CDS             complement(<1..>638)
                     /locus_tag="WGR46_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0857001.1"
                     /translation="PFFDKYGVRVSLLEDEDFIRAASMLAEEVFGFGIYKESKGSGGR
                     FYERCWLMGSEDALYGRVHFGGQNNTILFELTGTGCGVAKEGWESRLFAFLTNAIRPK
                     ITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKT
                     YGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGE"
BASE COUNT          151 a          157 c          145 g          185 t
ORIGIN      
        1 tattcgcccg gattctgcaa aacttcaaaa gggataacga tgtcttttgc tttgaattca
       61 atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt ttcatagacg
      121 cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt ggtcattttg
      181 gcttcatcgt cttcttccca atctgacccc aaacattcgc cttttggttt gacgtgatga
      241 caggtaaaca tacctttatt tcggtcttca cgggcttggt tcgggctgta ttcgccgttg
      301 aaaaagtctt ttgcgatgtc aacgcgtgtg atttttgggc ggattgcatt agtcaggaat
      361 gcgaaaagtc gtgattccca gccttctttt gcgacgccgc aaccggtgcc ggtcagttcg
      421 aaaagaatgg tattgttttg tccgccaaaa tggacgcgac cgtatagggc gtcttccgaa
      481 cccatcaacc aacagcgctc ataaaaacgc ccgcccgagc ctttggattc tttgtagata
      541 ccgaaaccga aaacttcttc ggcgagcatt gacgcggcgc gaataaaatc ttcgtcttcc
      601 aaaagactta cacgaacgcc gtatttatcg aaaaaggg
//
LOCUS       DAWFKN010000180          591 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFKN010000180 DAWFKN010000000
VERSION     DAWFKN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.180"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>591
                     /locus_tag="WGR46_002161"
     CDS             <1..>591
                     /locus_tag="WGR46_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0857002.1"
                     /translation="ITHNYPEPTGADKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGW
                     RIAADYASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAASSLGLSAIYDF
                     KLNDKFDKFKPYIGARVAYGHVKHQVHSVEKETEIVTTVPTGGAQTGGTIPQGPTPKP
                     PYHESNSISSLGLGVIAGVGFDITPKLTLDTGYRYHN"
BASE COUNT          187 a          166 c          126 g          112 t
ORIGIN      
        1 attacccaca attatccgga accaaccggt gcagacaaag acaaaataag cacagtaagc
       61 gattatttca gaaacatccg tgcgcattcc atccaccccc gggtgtcggt cggctacgat
      121 ttcggcggct ggagaatagc ggcagattat gccagttaca gaaaatggaa caacaataaa
      181 tattccgtca acacaaaaaa ggtgcaagaa aaccaaggca ccagaaaaga cctgaagacg
      241 gaaaatcagg aaaacggcag cttccacgcc gcttcttctc tcggcttatc cgccatttac
      301 gatttcaaac tcaacgataa attcgataaa ttcaaaccct atatcggcgc gcgtgtcgcc
      361 tacggacacg ttaaacatca ggttcattca gtggaaaaag aaaccgagat tgttaccact
      421 gtaccaacgg gaggcgctca aacgggaggc actatcccac aaggtccgac ccccaaacct
      481 ccctatcacg aaagcaacag catcagcagc ttgggtcttg gtgtcatcgc cggtgtcggt
      541 ttcgacatca cgcccaagct gaccttggac accggatacc gctaccacaa c
//
LOCUS       DAWFKN010000181          573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFKN010000181 DAWFKN010000000
VERSION     DAWFKN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.181"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>573
                     /locus_tag="WGR46_002162"
     CDS             <1..>573
                     /locus_tag="WGR46_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0857003.1"
                     /translation="YRNWLAQDNTLSELLELINCQLTEKGLKVEKASAAVIDATIIQT
                     AGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLH
                     ITPANAHECKHLPPLLEGLPKDTTVYADKGYDSAENRQHLKEHQLQDGIMRKACRNRP
                     LTEAQTKRNRYLSKTRYVVEQSFGTLHRKFR"
BASE COUNT          180 a          166 c          135 g           92 t
ORIGIN      
        1 taccgcaact ggctggcgca agacaatacc ctgtccgaat tgctggaact gattaactgc
       61 caactgaccg aaaaaggctt aaaagtagag aaagcatccg ccgccgtcat tgacgccacc
      121 attattcaga ccgctggcag caaacagcgt caggccatag aagttgacga agaaggacaa
      181 atcagcggcc aaaccacacc gagtaaggac agcgatgccc gttggatcaa gaaaaacggc
      241 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
      301 ctgcacatca cccccgccaa tgcccatgag tgcaaacacc tgccgccttt gttggaagga
      361 ctgcccaaag atacgaccgt ctatgccgac aaaggctacg acagtgcgga aaaccggcaa
      421 catctgaaag aacatcagtt gcaggacggc attatgcgca aagcctgccg caaccgtccg
      481 ctgacggaag cgcaaaccaa acgcaaccga tatttgtcga agacccgtta tgtggtcgaa
      541 caaagcttcg gtacgctgca ccgcaaattc cgc
//
LOCUS       DAWFKN010000182          538 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFKN010000182 DAWFKN010000000
VERSION     DAWFKN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.182"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            36..>538
                     /locus_tag="WGR46_002163"
     CDS             36..>538
                     /locus_tag="WGR46_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0857004.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKK"
BASE COUNT          177 a          105 c          105 g          151 t
ORIGIN      
        1 aatatattct ttaactaatt tctaagcttg aaattatgag accatatgct actaccattt
       61 atcaactttt tattttgttt attgggagtg tttttactat gacctcatgt gaacctgtga
      121 atgaaaagac agatcaaaaa gcagtaagtg cgcaacaggc taaagaacaa accagtttca
      181 acaatcccga gccaatgaca ggatttgaac atacggttac atttgatttt cagggcacca
      241 aaatggttat cccctatggc tatcttgcac ggtatacgca agacaatgcc acaaaatggc
      301 tttctgacac gcccgggcag gatgcttact ccattaattt gatagagatt agcgtctatt
      361 acaaaaaaac cgaccaaggc tgggttcttg agccatacaa ccagcaaaac aaagcacact
      421 ttatccaatt tctacgcgac ggtttggata gcgtggacga tattgttatc cgaaaagatg
      481 cgtgtagttt aagcacgact atgggagaaa gattgcttac ttacggggtt aaaaaaat
//
LOCUS       DAWFKN010000183          508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFKN010000183 DAWFKN010000000
VERSION     DAWFKN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.183"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            323..>508
                     /locus_tag="WGR46_002164"
     CDS             323..>508
                     /locus_tag="WGR46_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0857005.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRH"
BASE COUNT          151 a          126 c          124 g          107 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgagctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacac
//
LOCUS       DAWFKN010000184          475 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFKN010000184 DAWFKN010000000
VERSION     DAWFKN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.184"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(141..>475)
                     /locus_tag="WGR46_002165"
     CDS             complement(141..>475)
                     /locus_tag="WGR46_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0857006.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQQ
                     PLLVTPSA"
BASE COUNT          150 a           99 c           84 g          142 t
ORIGIN      
        1 gcgcattttt atgcgtcaaa tttcgttaac agactatttt tgcaaaggtc tcgaccctta
       61 tcccaatatt aagaaaaaca gcaatcaaaa taaacttgat tgctgttttt cagtttaagc
      121 catgaatgtg attaggctcc ttatgcagat ggcgttacca ataacggctg ctgtacggat
      181 tgtatattgg catccacatt tcctcctgtc gcagcagtat tagaaccgaa cacagacata
      241 gactgtacca aattattgcc agcattaata tacttggcaa aatctggatt actgatagct
      301 gcatcatcaa agacaaatgt atctacacga tggttttcac catagaagaa tccggaaata
      361 cgtacgttgt cttgttcaga agcgctaagc accaaatcac ttccggaacg gataaaatga
      421 acatctgctg ctttaaatcc tttaaagtgc atagtgtcag agtttttatc cacat
//
LOCUS       DAWFKN010000185          441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFKN010000185 DAWFKN010000000
VERSION     DAWFKN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.185"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            18..338
                     /locus_tag="WGR46_002166"
     CDS             18..338
                     /locus_tag="WGR46_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0857007.1"
                     /translation="MGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGYDEIK
                     AKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLAML
                     KFNS"
BASE COUNT          150 a           76 c          105 g          110 t
ORIGIN      
        1 taatcgacgc atcgggcttg ggcgaaaaaa ttaaagacgg caaaaaaacc gtactttcct
       61 gcgaagaaga acaaaaaatc tgcaatactt tcacgaacaa acaggcagtg gaagatttca
      121 gtgtggtaat cggctacgat gaaatcaaag cgaagaatca cagcttgtcg gcggggcagt
      181 atttcgaggt aaagattgat tatgtggata tttccgccga cgaatttgcg caaaaaatgg
      241 cgggattttc agcggatttg gataaacttt tcgccgaatc tgccgaattg gaaaaagaga
      301 ttaaggatag attggcgatg ttgaaattca attcataaat accgatgaat gaaaccggtc
      361 aaacttaaaa tgccgtctga aagcctttca gacggcattt tgtttggttt ggaaaatact
      421 tatgccgaga cctttgcaaa a
//
LOCUS       DAWFKN010000186          440 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFKN010000186 DAWFKN010000000
VERSION     DAWFKN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.186"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>440
                     /locus_tag="WGR46_002167"
     CDS             <1..>440
                     /locus_tag="WGR46_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0857008.1"
                     /translation="DTLSELLELINCQLTEKGLKVEKASAAVIDATIIQTAGSEQRQA
                     IEVDEEGQVSGQTTLSKDKNARWTKKNSLYKLGYKQHTRTDAEGYIEKLYITPTNAHE
                     CTHLSPLLEGLPKGTTVYADKGYDSKENRQHLKEHRLQNGIMRKA"
BASE COUNT          147 a          120 c          103 g           70 t
ORIGIN      
        1 gacaccctgt ccgaactgtt ggaactgatt aactgccaac tgaccgaaaa aggcttaaaa
       61 gtagagaaag catccgccgc cgtcattgac gccaccatta ttcagaccgc cggcagcgaa
      121 cagcgtcagg ccatagaagt cgatgaagag ggacaagtca gcggtcaaac cacactgagt
      181 aaggacaaaa atgcccgttg gacaaagaaa aacagcctct acaaactcgg ttacaaacaa
      241 catacccgta ccgatgcgga aggctatatc gagaaactgt acatcactcc caccaatgcc
      301 catgagtgca cacacctgtc gccgttgttg gaaggactgc ccaaaggtac gaccgtctat
      361 gccgacaaag gctacgacag caaggaaaac cggcaacatc tgaaagaaca tcggttgcag
      421 aacggcatta tgcgcaaagc
//
LOCUS       DAWFKN010000187          432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFKN010000187 DAWFKN010000000
VERSION     DAWFKN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.187"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGR46_002168"
     CDS             <1..318
                     /locus_tag="WGR46_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0857009.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEH
                     TIRDEADFSCHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGMYPHNWGGGNADFSI
                     EYD"
BASE COUNT          118 a           98 c          103 g          113 t
ORIGIN      
        1 catctgcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaac atactatccg cgatgaggcc gatttttcct gccattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttctg attggcggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatgtatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattgaat atgattaaag taaagtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggt tgcagaacgg catgccgcaa ccgtccgctg acggaaacgc aaaccaaacg
      421 taaccgatat tt
//
LOCUS       DAWFKN010000188          428 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFKN010000188 DAWFKN010000000
VERSION     DAWFKN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.188"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..173
                     /locus_tag="WGR46_002169"
     CDS             <1..173
                     /locus_tag="WGR46_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0857010.1"
                     /translation="LIMGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQE
                     MLKRHLQTKSVE"
     gene            176..412
                     /locus_tag="WGR46_002170"
     CDS             176..412
                     /locus_tag="WGR46_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0857011.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
BASE COUNT          103 a           88 c          140 g           97 t
ORIGIN      
        1 cgttaatcat gggacaggtt gcgttttacg aaaagatgat tgggctgtgg tcggccaaaa
       61 gccgtgaggc aagcgaacag gcagacttgg cggcgtttga atttgcggag ggcgaactgg
      121 ccaattatca ggaaatgctg aaacggcacc tgcaaaccaa aagtgtggaa tagcaatgcg
      181 tattttggat atttttaaaa acccagcgac aggcaatgtg tcgcactcga aactgtgggc
      241 aaacgttgcc tgcgcggcgg ggacggttaa gtttgtgatg ctgcccgacc cgtcggcgga
      301 gatttgggcg gtgtatttgg gcattgtcgg cggctatgcg gtggcgcgtt cgttggtcag
      361 cgtcaaacgt caggaggtcg agaatgaatc tcgtgaaact gctggcgaat aactggcaac
      421 cgattgcc
//
LOCUS       DAWFKN010000189          424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFKN010000189 DAWFKN010000000
VERSION     DAWFKN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.189"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            65..>424
                     /gene="mafA"
                     /locus_tag="WGR46_002171"
     CDS             65..>424
                     /gene="mafA"
                     /locus_tag="WGR46_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0857012.1"
                     /translation="MKILLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTS"
BASE COUNT          108 a          161 c           82 g           73 t
ORIGIN      
        1 ttcgtttaca aacccttaaa atccatttac actcaaaccc gttcaaaaca aaacaaaccc
       61 tgccatgaaa atcctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac
      121 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
      181 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
      241 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
      301 tatcgacgca ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag
      361 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
      421 ttcc
//
LOCUS       DAWFKN010000190          406 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFKN010000190 DAWFKN010000000
VERSION     DAWFKN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.190"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          115 a           83 c           88 g          120 t
ORIGIN      
        1 tctgcttttt ccaataaatg cccccaacct aaaatccgtc attcccgcgt aggcgtgaat
       61 ctagacattc aatgctaagg caatttatcg gaaatgactg aaactcaaaa aactggattc
      121 ccactttcgt gggaatgacg ggatgcaggt ttccgtatgg atggattcgt cattcccgcg
      181 caggcgggaa tctaggtctg tcagtgcgga aacttatcag gtaaaacggt ttcttgagat
      241 tttgcgtcct ggattcccac tttcgtggga atgacgcgat tagagtttca aaatttattc
      301 taaatagctg aaactcaatg aaccggattc ccgcttgcgc gggaatgacg ggattaagtt
      361 ttcaaaattc atcagaaatt actgatttaa tagcataaat ttttta
//
LOCUS       DAWFKN010000191          355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFKN010000191 DAWFKN010000000
VERSION     DAWFKN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.191"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          103 a           97 c           71 g           84 t
ORIGIN      
        1 cattgaaatt ctagattccc gcctgcgcgg aaatgacgaa tccatccgca cggaaacctg
       61 caccgcgtca ttcccacgaa cccacatccc gtcattacca cgaaagcggg aatctaggac
      121 gcagggttaa gaaaacctgc atcccgtcat tcctcaaaaa cagaaaacca aaatcagaaa
      181 cctaaaatcc cgtcattccc gcgcaggcgg gaatctaggt ctgtcagtgc aggaacttat
      241 cggataaaac ggtttcttga gattttacat cctggattcc cactttcgtg ggaatctaga
      301 atctcggact ttcagataat ctttgaatat tgccgctgcc ttaaggtcta gattc
//
LOCUS       DAWFKN010000192          354 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFKN010000192 DAWFKN010000000
VERSION     DAWFKN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.192"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT           86 a           92 c           77 g           99 t
ORIGIN      
        1 cgtcattccc acgaaagtgg gaatccagtt tcttgagttt cagtcattcc cgataaattg
       61 ccttagcatt gaatgtctag attcccgcct gcgcgggaat gacgaatcca tccatacgga
      121 aacctgcatc ccgtcattcc cacgaacctg tatcccgtca ttcccacgaa agtgggaatc
      181 cagctttttg agtttcagtc atttccgata aattgcctta acattgaatg tctagattcc
      241 cgcctgcgcg ggaatgacgg gatttgaaat tgcggcattt atcgggagca acagaagccg
      301 ctctgccgtc attcccacga aagtgggaat ccagcttttt gagtttcagt catt
//
LOCUS       DAWFKN010000193          349 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFKN010000193 DAWFKN010000000
VERSION     DAWFKN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.193"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..152)
                     /locus_tag="WGR46_002172"
     CDS             complement(<1..152)
                     /locus_tag="WGR46_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0857013.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEI"
BASE COUNT           93 a           80 c           81 g           95 t
ORIGIN      
        1 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgcaa agccaaacgc
       61 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      121 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      181 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      241 taaaggtttt gtcggataca agtatccgac ctacgcttgc tgaaccgtca ttcccacgga
      301 agtgggaatc tagaacgcgg ggtttcagtc atttccgata gattcccgc
//
LOCUS       DAWFKN010000194          347 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFKN010000194 DAWFKN010000000
VERSION     DAWFKN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.194"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            109..294
                     /locus_tag="WGR46_002173"
     CDS             109..294
                     /locus_tag="WGR46_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0857014.1"
                     /translation="MDGFVVPAKAGIRNQKPQKFIGKTETQKQRKPNGQDSRLRGNDG
                     SYITQKIETKQMGFPLA"
BASE COUNT           93 a           78 c           94 g           82 t
ORIGIN      
        1 cccgcctgcg cgggaatgac gggattttag gtttctgatt ttggttttct gtccttgtgg
       61 gaatgacggg atgtaggttc gtaggaatga cgtggtgcag gtttccgtat ggatggattc
      121 gtcgttcccg cgaaagcggg aatccggaac cagaaaccac aaaaatttat cgggaaaacc
      181 gaaactcaaa aacaacggaa accgaacgga caggattccc gcctgcgcgg gaatgacggc
      241 tcatacatca cccaaaaaat tgaaaccaaa cagatgggat tccctcttgc gtgaggctga
      301 cagatgccgt ctgaaagact ttcagacggc atagcttttt ctctttg
//
LOCUS       DAWFKN010000195          337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFKN010000195 DAWFKN010000000
VERSION     DAWFKN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.195"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            complement(145..337)
                     /locus_tag="WGR46_002174"
                     /pseudo
     CDS             complement(145..337)
                     /locus_tag="WGR46_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a           68 c           53 g           76 t
ORIGIN      
        1 cctctaaaaa ataatcgctt gttttgacac cgatacactc atatagtgga ttaacaaaaa
       61 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
      121 gtactggttt aaatttaatc cactatacaa atacagaaac tcaagaaaat aaccttgtgt
      181 attgaccatc tcaagcaatt cagaaaaatc aagaaatttt ctgaccgtaa acaaacgttt
      241 ccctaaaaaa acgatgtctt caaaaatatc gaacaaatag agacctttgc aaaaatagtc
      301 tgttaacgaa atttgacgca taaaaatgcg ccaaaaa
//
LOCUS       DAWFKN010000196          322 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFKN010000196 DAWFKN010000000
VERSION     DAWFKN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.196"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           96 c           74 g           50 t
ORIGIN      
        1 agtttgcagg attcggcgga aacggtaaaa acggcagaat cgatgggaat cggcaggctg
       61 aagcccaccc taccattatt tacacatccg taccgcttaa atgccgtctg aaacttcgtc
      121 attcccgtga aaacgggaat ccagccccgt gggagcagaa acttacaccc cgtcattccc
      181 gcgaacgcgg gaatccagta accgaaaaac cacaggaatc tatcggaaaa aacagaaccc
      241 cccgccgccg tcattcccgc gaacgcggga atctagtaac cgaaaaacca cgggaatcta
      301 tcggaaaaaa cggaaacccc cg
//
LOCUS       DAWFKN010000197          310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFKN010000197 DAWFKN010000000
VERSION     DAWFKN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.197"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            27..>310
                     /locus_tag="WGR46_002175"
     CDS             27..>310
                     /locus_tag="WGR46_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0857015.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAAGKAATGIEALSTV"
BASE COUNT           79 a           75 c           93 g           63 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgcgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacaggcaa aggcaatatg ccgctgcgtg aagccgtaaa actgattcgc ggctcggatg
      241 tggcgattga gttccatacc aacgcggcgg ccggtaaagc agctacaggc attgaggcgt
      301 tgagtaccgt
//
LOCUS       DAWFKN010000198          304 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFKN010000198 DAWFKN010000000
VERSION     DAWFKN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.198"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>304
                     /locus_tag="WGR46_002176"
     CDS             <1..>304
                     /locus_tag="WGR46_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0857016.1"
                     /translation="IAVLDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQN
                     LLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYG"
BASE COUNT           96 a           68 c           58 g           82 t
ORIGIN      
        1 gcattgccgt gcttgatgcc tacacggggc aggattccaa cacactctat tacatgagcg
       61 aggaagatgc gcttaatatc gtcaaagtaa ccaacgatac atacgaccat ctcgccaaaa
      121 acatctacca aaacctgtta ttccaaaccc gtttgcagcc atatttgaat caaatcagtt
      181 tcaaaatgga aaatgatacg ttcactttgg attttagtgg tcttgttcaa gcatttaacc
      241 atgtcaaaga aactaatccg caaaaagctt ttgtggattt ggccgagatg cttgcatatg
      301 gcga
//
LOCUS       DAWFKN010000199          293 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFKN010000199 DAWFKN010000000
VERSION     DAWFKN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.199"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
     gene            <1..>293
                     /gene="mafA"
                     /locus_tag="WGR46_002177"
     CDS             <1..>293
                     /gene="mafA"
                     /locus_tag="WGR46_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0857017.1"
                     /translation="GRKGERSAGLSVNGMGDYRNETLLANPRDVSFLTNLIQTVFYLR
                     GIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDS"
BASE COUNT           69 a          114 c           64 g           46 t
ORIGIN      
        1 gcggacgcaa aggcgaacgc tccgccggac tgtccgtcaa cggcatgggc gactaccgca
       61 acgaaaccct gctcgccaac ccccgcgacg tttccttcct gaccaacctc atccaaaccg
      121 tcttctacct gcgcggcatc gaagtcgtac cgcccgaata cgccgacacc gacgtattcg
      181 taaccgtcga cgtattcggc accgtccgca gccgtaccga actgcacctc tacaacgccg
      241 aaacccttaa agcccaaacc aagctcgaat atttcgccgt cgaccgcgac agc
//
LOCUS       DAWFKN010000200          292 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S95
            SAMN37720324-rid20220123.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFKN010000200 DAWFKN010000000
VERSION     DAWFKN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720324
            Sequence Read Archive: SRR26318829
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985303.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985303.1
            Genome Coverage       :: 238x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:06:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 62 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 15 of 139
            Pseudo Genes (multiple problems)  :: 16 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720324-rid20220123.denovo.200"
                     /strain="S95"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1975"
                     /collected_by="Suzhou CDC"
BASE COUNT           77 a           74 c           62 g           79 t
ORIGIN      
        1 tttggattcc cgcttgcgca ggaatgacgg tagagcggtt tcagttgctc ccgataaatg
       61 ccgcaatctc aaatcccgtc attccctcaa aaacagaaaa acaaaatcag aaacctaaaa
      121 tcccgtcatt cccgcagctc ttcgtcattc ccgtgaaagc gggaatctag accttttaaa
      181 ttttcacacg gcttttgaat attgccgtta cccgaaggtc tggattcccg cctgcacggg
      241 aatgacggca gagcggtttc agttgtttct aaataaattt ttgcagcgtt gg
//
